AU2006200807A1 - Coryneracterium glutamicum genes encoding metabolic pathway proteins - Google Patents

Description

P001 Section 29 Regulation 3.2(2)

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Coryneracterium glutamicum genes encoding metabolic pathway proteins The following statement is a full description of this invention, including the best method of performing it known to me us: 1 C1 CORYNEBACTERIUM GLUTAMICUM GENES ENCODING METABOLIC SPATHWAY PROTEINS Related Applications r The present application claims priority to prior filed U.S. Provisional Patent Application Serial No. 60/141031, filed June 25 1999, U.S. Provisional Patent Application Serial No. 60/142101, filed July 2, 1999, U.S. Provisional Patent oO 0Application Serial No. 60/148613, filed August 12, 1999, and also to U.S. Provisional Patent Application Serial No. 60/187970, filed March 9, 2000. The present application Nalso claims priority to prior filed German Patent Application No. 19930476.9, filed July 10 1, 1999, German Patent Application No. 19931415.2, filed July 8, 1999, German Patent Application No. 19931418.7, filed July 8, 1999, German Patent Application No.

19931419.5, filed July 8, 1999, German Patent Application No. 19931420.9, filed July 8, 1999, German Patent Application No. 19931424.1, filed July 8, 1999, German Patent Application No. 19931428.4, filed July 8, 1999, German Patent Application No.

19931434.9, filed July 8, 1999, German Patent Application No. 19931435.7, filed July 8, 1999, German Patent Application No. 19931443.8, filed July 8, 1999, German Patent Application No. 19931453.5, filed July 8, 1999, German Patent Application No.

19931457.8, filed July 8, 1999, German Patent Application No. 19931465.9, filed July 8, 1999, German Patent Application No. 19931478.0, filed July 8, 1999, German Patent Application No. 19931510.8, filed July 8, 1999, German Patent Application No.

19931541.8, filed July 8, 1999, German Patent Application No. 19931573.6, filed July 8, 1999, German Patent Application No. 19931592.2, filed July 8, 1999, German Patent Application No. 19931632.5, filed July 8, 1999, German Patent Application No.

19931634.1, filed July 8, 1999, German Patent Application No. 19931636.8, filed July 8, 1999, German Patent Application No. 19932125.6, filed July 9, 1999, German Patent Application No. 19932126.4, filed July 9, 1999, German Patent Application No.

19932130.2, filed July 9, 1999, German Patent Application No. 19932186.8, filed July 9, 1999, German Patent Application No. 19932206.6, filed July 9, 1999, German Patent Application No. 19932227.9, filed July 9, 1999, German Patent Application No.

19932228.7, filed July 9, 1999, German Patent Application No. 19932229.5, filed July 9, 1999, German Patent Application No. 19932230.9, filed July 9, 1999, German Patent Application No. 19932922.2, filed July 14, 1999, German Patent Application No.

19932926.5, filed July 14, 1999, German Patent Application No. 19932928.1, filed July 14, 1999, German Patent Application No. 19933004.2, filed July 14, 1999, German SPatent Application No. 19933005.0, filed July 14, 1999, German Patent Application No.

C19933006.9, filed July 14, 1999, German Patent Application No. 19940764.9, filed August 27, 1999, German Patent Application No. 19940765.7, filed August 27, 1999, German Patent Application No. 19940766.5, filed August 27, 1999, German Patent 00 Application No. 19940832.7, filed August 27, 1999, German Patent Application No.

S19941378.9, filed August 31, 1999, German Patent Application No. 19941379.7, filed IDAugust 31, 1999, German Patent Application No. 19941380.0, filed August 31, 1999, German Patent Application No. 19941394.0, filed August 31, 1999, German Patent Application No. 19941396.7, filed August 31, 1999, German Patent Application No.

19942076.9, filed September 3, 1999, German Patent Application No. 19942077.7, filed September 3, 1999, German Patent Application No. 19942079.3, filed September 3, 1999, German Patent Application No. 19942086.6, filed September 3, 1999, German Patent Application No. 19942087.4, filed September 3, 1999, German Patent Application No. 19942088.2, filed September 3, 1999, German Patent Application No.

19942095.5, filed September 3, 1999, German Patent Application No. 19942124.2, filed September 3, 1999, and German Patent Application No. 19942129.3, filed September 3, 1999. The entire contents of all of the aforementioned applications are hereby expressly incorporated herein by this reference.

Background of the Invention Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through large-scale culture of bacteria developed to produce and secrete large quantities of a particular desired molecule. One particularly useful organism for this purpose is Corynebacterium glutamicum, a gram positive, nonpathogenic bacterium. Through strain selection, a number of mutant strains have -3- CN been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.

Summary of the Invention The invention provides novel bacterial nucleic acid molecules which have a 0 variety of uses. These uses include the identification of microorganisms which can be Sused to produce fine chemicals, the modulation of fine chemical production in C.

glutamicum or related bacteria, the typing or identification of C. glutamicum or related bacteria, as reference points for mapping the C. glutamicum genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, referred to herein as metabolic pathway (MP) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The MP nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by fermentation processes. Modulation of the expression of the MP nucleic acids of the invention, or modification of the sequence of the MP nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).

The MP nucleic acids of the invention may also be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C.

glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium -4- Cl diphtheriae (the causative agent of diphtheria); the detection of such organisms is of Ssignificant clinical relevance.

The MP nucleic acid molecules of the invention may also serve as reference C points for mapping of the C. glutamicum genome, or of genomes of related organisms.

Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

SThe MP proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing an enzymatic step involved in the metabolism of certain fine chemicals, including amino acids, vitamins, cofactors, nutraceuticals, 10 nucleotides, nucleosides, and trehalose. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C. glutamicum and the related Brevibacterium species lactofermentum) (Yoshihama et al, J Bacteriol.

162: 591-597 (1985); Katsumata et J. Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals.

This improved production or efficiency of production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation. Specifically, alterations in C. glutamicum metabolic pathways for amino acids, vitamins, cofactors, nucleotides, and trehalose may have a direct impact on the overall production of one or more of these desired compounds from this organism. For example, optimizing the activity of a lysine biosynthetic pathway protein or decreasing the activity of a lysine degradative pathway protein may result in an increase in the yield or efficiency of production of lysine from such an engineered organism. Alterations in the proteins involved in these metabolic pathways may also have an indirect impact on the production or efficiency of production of a desired fine chemical. For example, a reaction which is in competition for an intermediate necessary for the production of a desired molecule may be eliminated, or a pathway necessary for the production of a particular intermediate for a desired compound may be optimized. Further, modulations in the biosynthesis or degradation of, for example, an amino acid, a vitamin, or a nucleotide may increase the overall ability of the microorganism to rapidly grow and divide, thus increasing the number and/or production capacities of the microorganism in culture and thereby increasing the Spossible yield of the desired fine chemical.

i The nucleic acid and protein molecules of the invention may be utilized to directly improve the production or efficiency of production of one or more desired fine chemicals from Corynebacterium glutamicum. Using recombinant genetic techniques 00 well known in the art, one or more of the biosynthetic or degradative enzymes of the invention for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, Ior trehalose may be manipulated such that its function is modulated. For example, a biosynthetic enzyme may be improved in efficiency, or its allosteric control region destroyed such that feedback inhibition of production of the compound is prevented.

Similarly, a degradative enzyme may be deleted or modified by substitution, deletion, or addition such that its degradative activity is lessened for the desired compound without impairing the viability of the cell. In each case, the overall yield or rate of production of the desired fine chemical may be increased.

It is also possible that such alterations in the protein and nucleotide molecules of the invention may improve the production of other fine chemicals besides the amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose through indirect mechanisms. Metabolism of any one compound is necessarily intertwined with other biosynthetic and degradative pathways within the cell, and necessary cofactors, intermediates, or substrates in one pathway are likely supplied or limited by another such pathway. Therefore, by modulating the activity of one or more of the proteins of the invention, the production or efficiency of activity of another fine chemical biosynthetic or degradative pathway may be impacted. For example, amino acids serve as the structural units of all proteins, yet may be present intracellularly in levels which are limiting for protein synthesis; therefore, by increasing the efficiency of production or the yields of one or more amino acids within the cell, proteins, such as biosynthetic or degradative proteins, may be more readily synthesized. Likewise, an alteration in a metabolic pathway enzyme such that a particular side reaction becomes more or less favored may result in the over- or under-production of one or more compounds which are utilized as intermediates or substrates for the production of a desired fine chemical.

SThis invention provides novel nucleic acid molecules which encode Sproteins, referred to herein as metabolic pathway proteins which are Scapable of, for example, performing an enzymatic step involved in the metabolism of molecules important for the normal functioning of cells, such as amino acids, vitamins, cofactors, nucleotides and nucleosides, or trehalose. Nucleic acid t molecules encoding an MP protein are referred to herein as MP nucleic acid 00 molecules. In a preferred embodiment, the MP protein performs an enzymatic step related to the metabolism of one or more of the following: amino acids, IND vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose.

0 10 Examples of such proteins include those encoded by the genes set forth in Table 1.

The following embodiments of the invention, the subject of this application, are specifically disclosed herein: SAn isolated Corynebacterium glutamicum nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

SAn isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleotide sequence of V any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid molecule does not consist of any of the F-designated Sgenes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic oo acid molecule does not consist of any of the F-designated genes set 0 0forth in Table 1, or a complement thereof.

0 An isolated polypeptide comprising an amino acid sequence 0 10 selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

An isolated polypeptide comprising an amino acid sequence which is at least 50% identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

e An isolated polypeptide comprising a fragment of a polypeptide Scomprising the amino acid sequence of any of the even numbered SSEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence.

O A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to IC1155, wherein the nucleic acid molecule is disrupted.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 1155.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

Accordingly, one aspect of the invention pertains to isolated nucleic acid molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an MP protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of MP encoding nucleic acid DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:I, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the isolated nucleic acidmolecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:I, SEQ ID N 6c

O

O NO:3, SEQ ID NO:5, SEQ ID or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid Ssequences set forth as an even-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID The preferred MP proteins of the present invention also preferably possess at least one of the MP activities described herein.

oo In another embodiment, the isolated nucleic acid molecule encodes a 0protein or portion thereof wherein the protein or portion thereof includes an amino IN acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence having an even-numbered SEQ ID NO: in the Sequence Listing), e.g., Ssufficiently homologous to an amino acid sequence of the invention such that the protein or portion thereof maintains an MP activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to perform an enzymatic F 5 reaction in a amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or 0 trehalose metabolic pathway. In one embodiment, the protein encoded by the nucleic Sacid molecule is at least about 50%, preferably at least about 60%, and more preferably IN at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of the invention an entire amino acid sequence selected from those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown in the corresponding odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID In another preferred embodiment, the isolated nucleic acid molecule is derived from C. glutamicum and encodes a protein an MP fusion protein) which inicludes a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered SEQ ID NOs in the Sequence Listing) and is able to catalyze a reaction in a metabolic pathway for an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose, or one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

In another embodiment, the isolated nucleic acid molecule is at least nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an oddnumbered SEQ ID NO in the Sequence Listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C glutamicum MP protein, or a biologically active portion thereof.

Another aspect of the invention pertains to vectors, recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which C such vectors have been introduced. In one embodiment, such a host cell is used to produce an MP protein by culturing the host cell in a suitable medium. The MP protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered 0 microorganism in which an MP gene has been introduced or altered. In one N embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated MP sequence as a transgene. In another embodiment, an endogenous MP gene within the genome of the microorganism has been altered, functionally disrupted, by homologous recombination with an altered MP gene. In another embodiment, an endogenous or introduced MP gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MP protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MP gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 1156) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated MP protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated MP protein or portion thereof can catalyze an enzymatic reaction involved in one or more pathways for the metabolism of an amino acid, a vitamin, a cofactor, a -9nutraceutical, a nucleotide, a nucleoside, or trehalose. In another preferred embodiment, l the isolated MP protein or portion thereof is sufficiently homologous to an amino acid N sequence of the invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction involved in one or more pathways for the metabolism of 00 San amino acid, a vitamin, a cofactor, a nutraceutical, a nucleotide, a nucleoside, or trehalose.

I The invention also provides an isolated preparation of an MP protein. In preferred embodiments, the MP protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded by an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing). In yet another embodiment, the protein is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated MP protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and is able to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more of the activities set forth in Table 1.

Alternatively, the isolated MP protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about 96%, 97%, or 99% or more homologous to a nucleotide sequence of one of the even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred that the preferred forms of MP proteins also have one or more of the MP bioactivities described herein.

The MP polypeptide, or a biologically active portion thereof, can be operatively Slinked to a non-MP polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the MP protein alone. In other preferred embodiments, this fusion protein, when introduced into a C glutamicum pathway for the metabolism of an amino acid, vitamin, cofactor, nutraceutical, results in 00 increased yields and/or efficiency of production of a desired fine chemical from C.

Sglutamicum. In particularly preferred embodiments, integration of this fusion protein u into an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose Smetabolic pathway of a host cell modulates production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an MP protein, either by interacting with the protein itself or a substrate or binding partner of the MP protein, or by modulating the transcription or translation of an MP nucleic acid molecule of the invention.

Another aspect of the invention pertains to a method for producing a fine chemical. This method involves the culturing of a cell containing a vector directing the expression of an MP nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an MP nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates MP protein activity or MP nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated for one or more C. glutamicum amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates MP protein activity can be an agent which stimulates MP protein activity or MP nucleic acid expression.

-11- Examples of agents which stimulate MP protein activity or MP nucleic acid expression Sinclude small molecules, active MP proteins, and nucleic acids encoding MP proteins that have been introduced into the cell. Examples of agents which inhibit MP activity or expression include small molecules, and antisense MP nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a 0desired compound from a cell, involving the introduction of a wild-type or mutant MP Sgene into a cell, either maintained on a separate plasmid or integrated into the genome of IDthe host cell. If integrated into the genome, such integration can be random, or it can Stake place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine.

Detailed Description of the Invention The present invention provides MP nucleic acid and protein molecules which are involved in the metabolism of certain fine chemicals in Corynebacterium glutamicum, including amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, such as C. glufamicum, either directly where modulation of the activity of a lysine biosynthesis protein has a direct impact on the production or efficiency of production of lysine from that organism), or may have an indirect impact which nonetheless results in an increase of yield or efficiency of production of the desired compound where modulation of the activity of a nucleotide biosynthesis protein has an impact on the production of an organic acid or a fatty acid from the bacterium, perhaps due to improved growth or an increased supply of necessary co-factors, energy compounds, or precursor molecules).

Aspects of the invention are further explicated below.

-12i. Fine Chemicals SThe term 'fine chemical' is art-recognized and includes molecules produced by cI an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and N related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references therein. The metabolism and uses of certain of these fine chemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term "amino acid" is artrecognized. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in proteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though Lamino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids 13have been well characterized in both prokaryotic and eukaryotic cells (see, for example, F Stryer, L. Biochemistry, 3 rd edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the r_ 5 complexity of their biosyntheses, are readily converted by simple biosynthetic pathways 00 to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, Ocysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals I do retain the ability to synthesize some of these amino acids, but the essential amino O acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also of monogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ Lmethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids technical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985.

The biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination of aketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a three- 14step process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and Sresulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain P-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

0 Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate Spathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step Sobiosynthetic pathway that differ only at the final two steps after synthesis ofprephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products ofpyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion ofaspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine from 5-phosphoribosyl-l-pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of the cell (for review see Stryer, L. Biochemistry 3 r d ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in terms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.

B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although they are readily synthesized by other organisms, such as bacteria. These molecules are Seither bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have 5 significant industrial value as coloring agents, antioxidants, and catalysts or other 00 0O processing aids. (For an overview of the structure, activity, and industrial applications Sof these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, S "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is art- Srecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamin (vitamin Bi) is produced by the chemical coupling of pyrimidine and thiazole moieties. Riboflavin (vitamin B 2 is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic -16acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl-1 -oxobutyl)-p-alanine) can be produced Seither by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to P- 5 alanine and for the condensation to panthotenic acid are known. The metabolically 00 active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in Senzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the \D precursors of Coenzyme A. These enzymes not only catalyze the formation of Spanthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, panthenol (provitamin Bs), pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and pamino-benzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as the cobalamines and particularly vitamin B12) and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

The biosynthesis of vitamin B 1 2 is sufficiently complex that it has not yet been completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed 'niacin'. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.

The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by largescale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and -17biotin. Only Vitamin B 12 is produced solely by fermentation, due to the complexity of Sits synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses 00 Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of 0 nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are nucleotides which do not form nucleic acid molecules, but rather serve as energy stores AMP) or as coenzymes FAD and NAD).

Several publications have described the use of these chemicals for these medical indications, by influencing purine and/or pyrimidine metabolism Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med. Res. Reviews 10: 505-548). Studies of enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or anti-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and -18- Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- S612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for 0 reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, S Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", SChapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from in a series of steps through the intermediate compound phosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or adenosine-5'-monophosphate (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate (CTP).

The deoxy- forms of all of these nucleotides are produced in a one step reduction reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

D. Trehalose Metabolism and Uses Trehalose consists of two glucose molecules, bound in a, a-l,l linkage. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- 467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from -19many microorganisms and is naturally released into the surrounding medium, from Swhich it can be collected using methods known in the art.

II. Elements and Methods of the Invention The present invention is based, at least in part, on the discovery of novel 00 molecules, referred to herein as MP nucleic acid and protein molecules, which play a Srole in or function in one or more cellular metabolic pathways. In one embodiment, the IN MP molecules catalyze an enzymatic reaction involving one or more amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways. In a preferred embodiment, the activity of the MP molecules of the present invention in one or more C. glutamicum metabolic pathways for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides or trehalose has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the MP molecules of the invention are modulated in activity, such that the C. glutamicum metabolic pathways in which the MP proteins of the invention are involved are modulated in efficiency or output, which either directly or indirectly modulates the production or efficiency of production of a desired fine chemical by C.

glutamicum.

The language, "MP protein" or "MP polypeptide" includes proteins which play a role in, catalyze an enzymatic reaction, in one or more amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside or trehalose metabolic pathways.

Examples of MP proteins include those encoded by the MP genes set forth in Table 1 and by the odd-numbered SEQ ID NOs. The terms "MP gene" or "MP nucleic acid sequence" include nucleic acid sequences encoding an MP protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence regions.

Examples of MP genes include those set forth in Table 1. The terms "production" or "productivity" are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given time and a given fermentation volume kg product per hour per liter). The term "efficiency of production" includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term "yield" or "product/carbon yield" is art-recognized and includes Sthe efficiency of the conversion of the carbon source into the product fine Schemical). This is generally written as, for example, kg product per kg carbon source.

By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a 00 "biosynthetic pathway" are art-recognized and include the synthesis of a compound, Spreferably an organic compound, by a cell from intermediate compounds in what may IND be a multistep and highly regulated process. The terms "degradation" or a "degradation Spathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language "metabolism" is art-recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound.

In another embodiment, the MP molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C. glutamicum. Using recombinant genetic techniques, one or more of the biosynthetic or degradative enzymes of the invention for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, or trehalose may be manipulated such that its function is modulated. For example, a biosynthetic enzyme may be improved in efficiency, or its allosteric control region destroyed such that feedback inhibition of production of the compound is prevented. Similarly, a degradative enzyme may be deleted or modified by substitution, deletion, or addition such that its degradative activity is lessened for the desired compound without impairing the viability of the cell. In each case, the overall yield or rate of production of one of these desired fine chemicals may be increased.

It is also possible that such alterations in the protein and nucleotide molecules of the invention may improve the production of other fine chemicals besides the amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose.

Metabolism of any one compound is necessarily intertwined with other biosynthetic and -21 Sdegradative pathways within the cell, and necessary cofactors, intermediates, or Ssubstrates in one pathway are likely supplied or limited by another such pathway.

STherefore, by modulating the activity of one or more of the proteins of the invention, the production or efficiency of activity of another fine chemical biosynthetic or degradative r 5 pathway may be impacted. For example, amino acids serve as the structural units of all 00 proteins, yet may be present intracellularly in levels which are limiting for protein Ssynthesis; therefore, by increasing the efficiency of production or the yields of one or IDmore amino acids within the cell, proteins, such as biosynthetic or degradative proteins, may be more readily synthesized. Likewise, an alteration in a metabolic pathway enzyme such that a particular side reaction becomes more or less favored may result in the over- or under-production of one or more compounds which are utilized as intermediates or substrates for the production of a desired fine chemical.

The isolated nucleic acid sequences of the invention are contained within the genome of a Corynebacterium glutamicum strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated C. glutamicum MP DNAs and the predicted amino acid sequences of the C.

glutamicum MP proteins are shown in the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode metabolic pathway proteins.

The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention the sequence of an even-numbered SEQ ID NO of the Sequence Listing). As used herein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

The MP protein or a biologically active portion or fragment thereof of the invention can catalyze an enzymatic reaction in one or more amino acid, vitamin, -22cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, or have Sone or more of the activities set forth in Table I.

SVarious aspects of the invention are described in further detail in the following subsections: 00 A. Isolated Nucleic Acid Molecules SOne aspect of the invention pertains to isolated nucleic acid molecules that IDencode MP polypeptides or biologically active portions thereof, as well as nucleic acid Sfragments sufficient for use as hybridization probes or primers for the identification or amplification of MP-encoding nucleic acid MP DNA). As used herein, the term "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5' end of the coding region and at least about nucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated MP nucleic acid molecule can contain less than about kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb ofnucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived a C. glutamicum cell). Moreover, an "isolated" nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the -23sequence information provided herein. For example, a C glutamicum MP DNA can be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, r- 5 T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor 00 Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

SMoreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic \0 acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by O the polymerase chain reaction using oligonucleotide primers designed based upon this sequence a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Sequence Listing) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979) Biochemistry 18: 5294-5299) and DNA can be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an MP nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic acid sequences of the invention, as set forth in the Sequence Listing, correspond to the Corynebacterium glutamicum MP DNAs of the invention. This DNA comprises sequences encoding MP proteins the "coding region", indicated in each oddnumbered SEQ ID NO: sequence in the Sequence Listing), as well as 5' untranslated -24sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID SNO: in the Sequence Listing. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the nucleic acid sequences of the Sequence Listing.

For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, 00 RXN, RXS, or RXC number having the designation "RXA", "RXN", "RXS", or "RXC" 0 followed by 5 digits RXA00007, RXN00023, RXS00116, or RXC00128). Each of IN the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding O region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, RXS, or RXC designation to eliminate confusion. The recitation "one of the odd-numbered sequences of the Sequence Listing", then, refers to any of the nucleic acid sequences in the Sequence Listing, which may also be distinguished by their differing RXA, RXN, RXS, or RXC designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also set forth in the Sequence Listing, as an even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence. For example, the coding region for RXA02229 is set forth in SEQ ID NO: 1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, RXS, or RXC designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequences designated RXA02229, RX00351, RXS02970, and RXC02390 are translations of the coding regions of the nucleotide sequences of nucleic acid molecules RXA02229, RX00351, RXS02970, and RXC02390, respectively. The correspondence between the RXA, RXN, RXS, and RXC nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in Table 1.

Several of the genes of the invention are "F-designated genes". An F-designated gene includes those genes set forth in Table 1 which have an in front of the RXA, RXN, RXS, or RXC designation. For example, SEQ ID NO:5, designated, as indicated on Table 1, as "F RXA01009", is an F-designated gene, as are SEQ ID NOs: 73, 75, and 77 (designated on Table 1 as "F RXA00007", "F RXA00364", and "F RXA00367", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not Sintended to include C. glutamicum those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, et al. (1998) J.

Bacteriol. 180(12): 3159-3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that 00 the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

IND In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences of the invention is one which is sufficiently complementary to one of the nucleotide sequences shown in the Sequence Listing the sequence of an odd-numbered SEQ ID NO:) such that it can hybridize to one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78% 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, e.g, hybridizes under stringent conditions, to one of the nucleotide sequences of the invention, or a portion thereof.

Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs -26of the Sequence Listing, for example a fragment which can be used as a probe or primer Sor a fragment encoding a biologically active portion of an MP protein. The nucleotide sequences determined from the cloning of the MP genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning MP r 5 homologues in other cell types and organisms, as well as MP homologues from other

O

00 Corynebacteria or related species. The probe/primer typically comprises substantially Spurified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide \0 sequence that hybridizes under stringent conditions to at least about 12, preferably about more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the oddnumbered SEQ ID NOs of the Sequence Listing), an anti-sense sequence of one of these sequences, or naturally occurring mutants thereof. Primers based on a nucleotide sequence of the invention can be used in PCR reactions to clone MP homologues.

Probes based on the MP nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g. the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme cofactor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an MP protein, such as by measuring a level of an MP-encoding nucleic acid in a sample of cells from a subject detecting MP mRNA levels or determining whether a genomic MP gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an evennumbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway. As used herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side chain as an amino acid residue in a sequence of one of the even-numbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the -27protein or portion thereof is able to catalyze an enzymatic reaction in a C. glutamicum Samino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside or trehalose Smetabolic pathway. Protein members of such metabolic pathways, as described herein, function to catalyze the biosynthesis or degradation of one or more of: amino acids, r 5 vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, or trehalose. Examples of 00 such activities are also described herein. Thus, "the function of an MP protein" 0 contributes to the overall functioning of one or more such metabolic pathway and N contributes, either directly or indirectly, to the yield, production, and/or efficiency of Sproduction of one or more fine chemicals. Examples of MP protein activities are set forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

Portions of proteins encoded by the MP nucleic acid molecules of the invention are preferably biologically active portions of one of the MP proteins. As used herein, the term "biologically active portion of an MP protein" is intended to include a portion, a domain/motif, of an MP protein that catalyzes an enzymatic reaction in one or more C. glutamicum amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, or has an activity as set forth in Table 1. To determine whether an MP protein or a biologically active portion thereof can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an MP protein can be prepared by isolating a portion of one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the MP protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the MP protein or peptide.

-28- The invention further encompasses nucleic acid molecules that differ from one of Sthe nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic code and thus encode the same MP protein as that encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the 00 invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further IND embodiment, the nucleic acid molecule of the invention encodes a full length C.

glutamicum protein which is substantially homologous to an amino acid sequence of the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or For example, the invention includes a nucleotide sequence which is greater than and/or at least 40% identical to the nucleotide sequence designated RXA00 15 (SEQ ID NO:185), a nucleotide sequence which is greater than and/or at least identical to the nucleotide sequence designated RXA00131 (SEQ ID NO:991), and a nucleotide sequence which is greater than and/or at least 39% identical to the nucleotide sequence designated RXA00219 (SEQ ID NO:345). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, -29- S74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, J 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about N 95%, 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C. glutamicum MP nucleotide sequences set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by one of 0 0 ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the Samino acid sequences of MP proteins may exist within a population the C.

IN glutamicum population). Such genetic polymorphism in the MP gene may exist among Sindividuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an MP protein, preferably a C. glutamicum MP protein. Such natural variations can typically result in 1-5% variance in the nucleotide sequence of the MP gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in MP that are the result of natural variation and that do not alter the functional activity of MP proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C. glutamicum homologues of the C. glutamicum MP DNA of the invention can be isolated based on their homology to the C. glutamicum MP nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of an odd-numbered SEQ ID NO: of the Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to one of ordinary skill in the art and can be found in Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6.

A preferred, non-limiting example of stringent hybridization conditions are C4 hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45C, followed by c1 one or more washes in 0.2 X SSC, 0.1% SDS at 50-65 0 C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid 00 0molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an SRNA or DNA molecule having a nucleotide sequence that occurs in nature Iencodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

0 glutamicum MP protein.

In addition to naturally-occurring variants of the MP sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into a nucleotide sequence of the invention, thereby leading to changes in the amino acid sequence of the encoded MP protein, without altering the functional ability of the MP protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the MP proteins an evennumbered SEQ ID NO: of the Sequence Listing) without altering the activity of said MP protein, whereas an "essential" amino acid residue is required for MP protein activity.

Other amino acid residues, however, those that are not conserved or only semiconserved in the domain having MP activity) may not be essential for activity and thus are likely to be amenable to alteration without altering MP activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding MP proteins that contain changes in amino acid residues that are not essential for MP activity. Such MP proteins differ in amino acid sequence from a sequence of an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the MP activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the invention and is capable of catalyzing an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more activities set forth in Table 1. Preferably, the protein encoded by the nucleic -31acid molecule is at least about 50-60% homologous to the amino acid sequence of one of Sthe odd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about 60-70% homologous to one of these sequences, even more preferably at least about 80-90%, 90-95% homologous to one of these sequences, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of 00 the invention.

To determine the percent homology of two amino acid sequences one of IND the amino acid sequences of the invention and a mutant form thereof) or of two nucleic Sacids, the sequences are aligned for optimal comparison purposes gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one sequence one of the amino acid sequences of the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of the amino acid sequence), then the molecules are homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences homology of identical positions/total of positions x 100).

An isolated nucleic acid molecule encoding an MP protein homologous to a protein sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic -32acid), uncharged polar side chains glycine, asparagine, glutamine, serine,

S

threonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, Sisoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains threonine, valine, isoleucine) and aromatic side chains tyrosine, t 5 phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue 0 in an MP protein is preferably replaced with another amino acid residue from the same Sside chain family. Alternatively, in another embodiment, mutations can be introduced N randomly along all or part of an MP coding sequence, such as by saturation Smutagenesis, and the resultant mutants can be screened for an MP activity described herein to identify mutants that retain MP activity. Following mutagenesis of the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).

In addition to the nucleic acid molecules encoding MP proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense! nucleic acid comprises a nucleotide sequence which is complementary to a "sense" nucleic acid encoding a protein, complementary to the coding strand of a double-stranded DNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire MP coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an MP protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues the entire coding region of SEQ ID NO. 1 (RXA02229) comprises nucleotides 1 to 825). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding MP. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding MP disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense -33nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of MP mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of MP mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding 0 00 the translation start site of MP mRNA. An antisense oligonucleotide can be, for 0 example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, S-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-Dgalactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, I-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomthyluracil, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2 -methylthio-N6-isopentenyladenine, acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

-34- The antisense nucleic acid molecules of the invention are typically administered Sto a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an MCT protein to thereby inhibit expression of the protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the 0case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can Sbe modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention is an ao-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual p-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

Res. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-omethylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

In still another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave MP mRNA transcripts to thereby inhibit translation of MP mRNA.

A ribozyme having specificity for an MP-encoding nucleic acid can be designed based upon the nucleotide sequence of an MP DNA disclosed herein SEQ ID NO: 1 (RXA02229). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an MP-encoding mRNA. See, Cech et al.

U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No. 5,116,742. Alternatively, MP SmRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

5 Alternatively, MP gene expression can be inhibited by targeting nucleotide

O

00 sequences complementary to the regulatory region of an MP nucleotide sequence 0 0 an MP promoter and/or enhancers) to form triple helical structures that prevent \0 transcription of an MP gene in target cells. See generally, Helene, C. (1991) Anticancer 0 Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N. Y Acad Sci. 660:27-36; and Maher, L.J. (1992) Bioassays 14(12):807-15.

B. Recombinant Expression Vectors and Host Cells Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an MP protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form ofplasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector.

However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

-36- The recombinant expression vectors of the invention comprise a nucleic acid of Sthe invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant 00 expression vector, "operably linked" is intended to mean that the nucleotide sequence of Ointerest is linked to the regulatory sequence(s) in a manner which allows for expression O of the nucleotide sequence in an in vitro transcription/translation system or in a O host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, repressor binding sites, activator binding sites, enhancers and other expression control elements terminators, polyadenylation signals, or other elements of mRNA secondary structure). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990).

Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells. Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacl q T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SPO2, X-PR- or X PL, which are used preferably in bacteria.

Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC1, MFa, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, usp, STLS1, B33, nos or ubiquitin- or phaseolinpromoters. It is also possible to use artificial promoters. It will be appreciated by one of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein MP proteins, mutant forms of MP proteins, fusion proteins, etc.).

The recombinant expression vectors of the invention can be designed for expression of MP proteins in prokaryotic or eukaryotic cells. For example, MP genes can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus -37expression vectors), yeast and other fungal cells (see Romanos, M.A. et al. (1992) S"Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer 0 systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), Salgae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High Sefficiency Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.: 583-586), or mammalian cells.

Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences, and T7 polymerase.

Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the Cterminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the MP protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from -38the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion Sprotein can be purified by affinity chromatography using glutathione-agarose resin.

Recombinant MP protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.

r 5 Examples of suitable inducible non-fusion E. coli expression vectors include

O

00 pTrc (Amann et al., (1988) Gene 69:301-315) pLG338, pACYC 184, pBR322, pUC18, O pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- 0 III 113-B1, Xgtl 1, pBdCl, and pET 1 id (Studier et al., Gene Expression Technology: SMethods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89; and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET Id vector relies on transcription from a T7 gnlO-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS174(DE3) from a resident K prophage harboring a T7 gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJl01, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUB 110, pC 194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM 1519, pBL 1, pSA77, or pAJ667 (Pouwels et al., eds.

(1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

One strategy to maximize recombinant protein expression is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as C. glutamicum (Wada et al. (1992) Nucleic Acids Res. 20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.

-39- In another embodiment, the MP protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, el al., (1987) Embo J. 6:229-234),, 2 t, pAG-1, Yep6, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and

O

00 methods for the construction of vectors appropriate for use in other fungi, such as the Sfilamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

0 (1991) "Gene transfer systems and vector development for filamentous fungi, in: SApplied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, the MP proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf 9 cells) include the pAc series (Smith et al.

(1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

In another embodiment, the MP proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC (Kaufman et al. (1987) EMBO J. 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.

For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., Sand Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In another embodiment, the recombinant mammalian expression vector is o capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissue- NO specific regulatory elements are known in the art. Non-limiting examples of suitable Stissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) Adv. Immunol. 43:235-275), in particular promoters of T cell receptors (Winoto and.

Baltimore (1989) EMBO J. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byrne and Ruddle (1989) PNAS 86:5473-5477), pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) Science 249:374-379) and the ac-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to MP mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell -41type into which the vector is introduced. For a discussion of the regulation of gene Sexpression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) 1986.

Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and 00 "recombinant host cell" are used interchangeably herein. It is understood that such 0 terms refer not only to the particular subject cell but to the progeny or potential progeny IN of such a cell. Because certain modifications may occur in succeeding generations due O to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an MP protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those of ordinary skill in the art. Microorganisms related to Corynebacterium glutamicum which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection", "conjugation" and "transduction" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector a plasmid, phage, phasmid, phagemid, transposon or other DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these -42integrants, a gene that encodes a selectable marker resistance to antibiotics) is Sgenerally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an MP protein or can be 00 introduced on a separate vector. Cells stably transfected with the introduced nucleic

O

O acid can be identified by drug selection cells that have incorporated the selectable Smarker gene will survive, while the other cells die).

STo create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an MP gene into which a deletion, addition or substitution has been introduced to thereby alter, functionally disrupt, the MP gene. Preferably, this MP gene is a Corynebacterium glutamicum MP gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source. In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous MP gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous MP gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the endogenous MP protein). In the homologous recombination vector, the altered portion of the MP gene is flanked at its 5' and 3' ends by additional nucleic acid of the MP gene to allow for homologous recombination to occur between the exogenous MP gene carried by the vector and an endogenous MP gene in a microorganism. The additional flanking MP nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see Thomas, and Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and cells in which the introduced MP gene has homologously recombined with the endogenous MP gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene.

-43- For example, inclusion of an MP gene on a vector placing it under control of the lac operon permits expression of the MP gene only in the presence of IPTG. Such N, regulatory systems are well known in the art.

In another embodiment, an endogenous MP gene in a host cell is disrupted F 5 by homologous recombination or other genetic means known in the art) such that 00 expression of its protein product does not occur. In another embodiment, an endogenous or introduced MP gene in a host cell has been altered by one or more point mutations, IDdeletions, or inversions, but still encodes a functional MP protein. In still another Sembodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MP gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described MP gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.

A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an MP protein. Accordingly, the invention further provides methods for producing MP proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an MP protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered MP protein) in a suitable medium until MP protein is produced. In another embodiment, the method further comprises isolating MP proteins from the medium or the host cell.

C. Isolated MP Proteins Another aspect of the invention pertains to isolated MP proteins, and biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized.

The language "substantially free of cellular material" includes preparations of MP protein in which the protein is separated from cellular components of the cells in which -44- Sit is naturally or recombinantly produced. In one embodiment, the language 1"substantially free of cellular material" includes preparations of MP protein having less than about 30% (by dry weight) of non-MP protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-MP protein, still more preferably less than about 10% of non-MP protein, and most preferably less than 00 about 5% non-MP protein. When the MP protein or biologically active portion thereof Sis recombinantly produced, it is also preferably substantially free of culture medium, i.e., INDculture medium represents less than about 20%, more preferably less than about Sand most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of MP protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of MP protein having less than about 30% (by dry weight) of chemical precursors or non-MP chemicals, more preferably less than about 20% chemical precursors or non-MP chemicals, still more preferably less than about 10% chemical precursors or non-MP chemicals, and most preferably less than about 5% chemical precursors or non-MP chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the MP protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum MP protein in a microorganism such as C. gluramicum.

An isolated MP protein or a portion thereof of the invention can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an MP protein of the invention has an amino acid sequence set forth as an even-numbered SEQ ID NO: of Sthe Sequence Listing. In yet another preferred embodiment, the MP protein has an CI amino acid sequence which is encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another 00 preferred embodiment, the MP protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, IN 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred MP proteins of the present invention also preferably possess at least one of the MP activities described herein. For example, a preferred MP protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention, and which can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or which has one or more of the activities set forth in Table 1.

In other embodiments, the MP protein is substantially homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and retains the functional activity of the protein of one of the amino acid sequences of the invention yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the MP protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, -46- 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or S91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, S99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the MP activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) 00 are also intended to be encompassed by the present invention. For example, ranges of Sidentity values using a combination of any of the above values recited as upper and/or IClower limits are intended to be included. In another embodiment, the invention pertains Sto a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

Biologically active portions of an MP protein include peptides comprising amino acid sequences derived from the amino acid sequence of an MP protein, an amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing or the amino acid sequence of a protein homologous to an MP protein, which include fewer amino acids than a full length MP protein or the full length protein which is homologous to an MP protein, and exhibit at least one activity of an MP protein.. Typically, biologically active portions (peptides, peptides which are, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an MP protein. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an MP protein include one or more selected domains/motifs or portions thereof having biological activity.

MP proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the MP protein is expressed in the host cell. The MP protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an MP protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native MP protein can be isolated from cells endothelial -47cells), for example using an anti-MP antibody, which can be produced by standard Stechniques utilizing an MP protein or fragment thereof of this invention.

SThe invention also provides MP chimeric or fusion proteins. As used herein, an MP "chimeric protein" or "fusion protein" comprises an MP polypeptide operatively 5 linked to a non-MP polypeptide. An "MP polypeptide" refers to a polypeptide having an 00 amino acid sequence corresponding to MP, whereas a "non-MP polypeptide" refers to a 0 polypeptide having an amino acid sequence corresponding to a protein which is not NO substantially homologous to the MP protein, a protein which is different from the O MP protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the MP polypeptide and the non-MP polypeptide are fused in-frame to each other. The non-MP polypeptide can be fused to the N-terminus or C-terminus of the MP polypeptide. For example, in one embodiment the fusion protein is a GST-MP fusion protein in which the MP sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant MP proteins. In another embodiment, the fusion protein is an MP protein containing a heterologous signal sequence at its Nterminus. In certain host cells mammalian host cells), expression and/or secretion of an MP protein can be increased through use of a heterologous signal sequence.

Preferably, an MP chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers.

Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide). An MP- -48encoding nucleic acid can be cloned into such an expression vector such that the fusion Smoiety is linked in-frame to the MP protein.

Homologues of the MP protein can be generated by mutagenesis, discrete point mutation or truncation of the MP protein. As used herein, the term "homologue" 5 refers to a variant form of the MP protein which acts as an agonist or antagonist of the activity of the MP protein. An agonist of the MP protein can retain substantially the same, or a subset, of the biological activities of the MP protein. An antagonist of the u MP protein can inhibit one or more of the activities of the naturally occurring form of Sthe MP protein, by, for example, competitively binding to a downstream or upstream member of the MP cascade which includes the MP protein. Thus, the C. glutamicum MP protein and homologues thereof of the present invention may modulate the activity of one or more metabolic pathways in which MP proteins play a role in this microorganism.

In an alternative embodiment, homologues of the MP protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the MP protein for MP protein agonist or antagonist activity. In one embodiment, a variegated library of MP variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of MP variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential MP sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of MP sequences therein.

There are a variety of methods which can be used to produce libraries of potential MP homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential MP sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477.

-49- .C In addition, libraries of fragments of the MP protein coding can be used to Sgenerate a variegated population of MP fragments for screening and subsequent selection of homologues of an MP protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an MP coding sequence with a nuclease under conditions wherein nicking occurs only 00 about once per molecule, denaturing the double stranded DNA, renaturing the DNA to O form double stranded DNA which can include sense/antisense pairs from different <0 nicked products, removing single stranded portions from reformed duplexes by O treatment with S1 nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of tie.MP protein.

Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of MP homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify MP homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

In another embodiment, cell based assays can be exploited to analyze a variegated MP library, using methods well known in the art.

D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of C glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

glutamicum sequences of interest; evolutionary studies; determination of MP protein regions required for function; modulation of an MP protein activity; modulation of the C, activity of an MP pathway; and modulation of cellular production of a desired compound, such as a fine chemical.

The MP nucleic acid molecules of the invention have a variety of uses. First, Sthey may be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof. Also, they may be used to identify the presence of C. gluramicum Sor a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C. glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is not pathogenic to humans, it is related to species which are human pathogens, such as Corynebacterium diphtheriae.

Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990.

In one embodiment, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth as odd-numbered or even-numbered SEQ ID NOs, respectively, in the Sequence Listing) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject. C. glutamicum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glutamicum -51 Iare homologous to C. diphiheriae nucleic acid and protein molecules, and can therefore Fbe used to detect C. diphiheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of t. 5 the genome, but also for functional studies of C. glutamicum proteins. For example, to 00 identify the region of the genome to which a particular C glutamicum DNA-binding 00 O protein binds, the C. glutamicum genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed O with the nucleic acid molecules of the invention, preferably with readily detectable 10 labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction ofa genomic map in related bacteria, such as Brevibacterium lactofermentum.

The MP nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The metabolic processes in which the molecules of the invention participate are utilized by a wide variety ofprokaryotic and eukaryotic cells; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms of mutagenesis without losing function.

Manipulation of the MP nucleic acid molecules of the invention may result in the production of MP proteins having functional differences from the wild-type MP proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

-52- SThe invention also provides methods for screening molecules which modulate Sthe activity of an MP protein, either by interacting with the protein itself or a substrate or binding partner of the MP protein, or by modulating the transcription or translation of an MP nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more MP proteins of the invention is contacted with one or more test 00 compounds, and the effect of each test compound on the activity or level of expression of the MP protein is assessed.

IND When the desired fine chemical to be isolated from large-scale fermentative culture of C. glutamicum is an amino acid, a vitamin, a cofactor, a nutraceutical, a nucleotide, a nucleoside, or trehalose, modulation of the activity or efficiency of activity of one or more of the proteins of the invention by recombinant genetic mechanisms may directly impact the production of one of these fine chemicals. For example, in the case of an enzyme in a biosynthetic pathway for a desired amino acid, improvement in efficiency or activity of the enzyme (including the presence of multiple copies of the gene) should lead to an increased production or efficiency of production of that desired amino acid. In the case of an enzyme in a biosynthetic pathway for an amino acid whose synthesis is in competition with the synthesis of a desired amino acid, any decrease in the efficiency or activity of this enzyme (including deletion of the gene) should result in an increase in production or efficiency of production of the desired amino acid, due to decreased competition for intermediate compounds and/or energy. In the case of an enzyme in a degradation pathway for a desired amino acid, any decrease in efficiency or activity of the enzyme should result in a greater yield or efficiency of production of the desired product due to a decrease in its degradation. Lastly, mutagenesis of an enzyme involved in the biosynthesis of a desired amino acid such that this enzyme is no longer is capable of feedback inhibition should result in increased yields or efficiency of production of the desired amino acid. The same should apply to the biosynthetic and degradative enzymes of the invention involved in the metabolism of vitamins, cofactors, nutraceuticals, nucleotides, nucleosides and trehalose.

Similarly, when the desired fine chemical is not one of the aforementioned compounds, the modulation of activity of one of the proteins of the invention may still impact the yield and/or efficiency of production of the compound from large-scale culture of C. glulamicum. The metabolic pathways of any organism are closely -53interconnected; the intermediate used by one pathway is often supplied by a different Spathway. Enzyme expression and function may be regulated based on the cellular levels of a compound from a different metabolic process, and the cellular levels of molecules necessary for basic growth, such as amino acids and nucleotides, may critically affect the viability of the microorganism in large-scale culture. Thus, modulation of an amino 00 acid biosynthesis enzyme, for example, such that it is no longer responsive to feedback inhibition or such that it is improved in efficiency or turnover may result in increased IDcellular levels of one or more amino acids. In turn, this increased pool of amino acids provides not only an increased supply of molecules necessary for protein synthesis, but also of molecules which are utilized as intermediates and precursors in a number of other biosynthetic pathways. If a particular amino acid had been limiting in the cell, its increased production might increase the ability of the cell to perform numerous other metabolic reactions, as well as enabling the cell to more efficiently produce proteins of all kinds, possibly increasing the overall growth rate or survival ability of the cell in large scale culture. Increased viability improves the number of cells capable of producing the desired fine chemical in fermentative culture, thereby increasing the yield.

of this compound. Similar processes are possible by the modulation of activity of a degradative enzyme of the invention such that the enzyme no longer catalyzes, or catalyzes less efficiently, the degradation of a cellular compound which is important for the biosynthesis of a desired compound, or which will enable the cell to grow and reproduce more efficiently in large-scale culture. It should be emphasized that optimizing the degradative activity or decreasing the biosynthetic activity of certain molecules of the invention may also have a beneficial effect on the production of certain fine chemicals from C. glutamicum. For example, by decreasing the efficiency of activity of a biosynthetic enzyme in a pathway which competes with the biosynthetic pathway of a desired compound for one or more intermediates, more of those intermediates should be available for conversion to the desired product. A similar situation may call for the improvement of degradative ability or efficiency of one or more proteins of the invention.

This aforementioned list of mutagenesis strategies for MP proteins to result in increased yields of a desired compound is not meant to be limiting; variations on these mutagenesis strategies will be readily apparent to one of ordinary skill in the art. By -54these mechanisms, the nucleic acid and protein molecules of the invention may be H utilized to generate C. glutamicum or related strains of bacteria expressing mutated MP 1 nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any 5 natural product of C. glutamicum, which includes the final products of biosynthesis 00 pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of C. glutamicum, but which Sare produced by a C. glutamicum strain of the invention.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence'listing cited throughout this application are hereby incorporated by reference.

2006200807 24 Feb 2006 TABLE 1: Included Genes Lysine biosynthesis Nucleic Acid SEQ ID NO 3 7 Amino Acid SEQ ID NO 4 6 8 12 Identification Code RXA02229 RXS02970 F RXA01 009 RXC02390 RXCO1 796 RXCO1 207 RXC00657 RXCO0552 Identification Code RXN00351 F RXA00351 RXA00873 RXA00891 GR00653 GR00287 NT Start 2793 4714 NT Stop 3617 5943 Function DIAMINOPIMELATE EPIMERASE (EC 5.1.1.7) ACETYLORNITHINE AMINOTRANSFE RASE (EC 2.6. 1.11) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) MEMBRANE SPANNING PROTEIN INVOLVED IN LYSINE METABOLISM MEMBRANE ASSOCIATED PROTEIN INVOLVED IN LYSINE METABOLISM CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF LYSINE AND

THREONINE

TRANSCRIPTIONAL REGULATOR INVOLVED IN LYSINE METABOLISM CYTOSOLIC PROTEIN INVOLVED IN LYSINE METABOLISM Treh al ose Nucleic Acid Amino Acid SEQ ID NO SEQ IDNO 17 18 19 20 21 22 23 24 9o22ag W0135 GROO066 GR00241 GR00243 NIT Start 37078 1486 3 1005 NT Stop Function 38532 ALPHA,ALPHATREHALOSEPHOPHATE SYNTHASE (UDP-FORMING) 56 KID SUBUNIT (EC 2.4.1.15) 2931 ALPHA.ALPHA.TREHALOSE-PHOSPHATE SYNTH-ASE (UDP-FORMING) 56 KD SUBUNIT (EC 2.4. 1. 15) 758 trehalose synthase (EC 4 trehalose synthase (EC Lysine biosynthesis Nucleic Acid SEQ ID NO 27 29 Amino Acid SEQ ID NO 26- 28 30.

Identification Code RXA00534 RXA00533 RXA02843 RXA02022 RXAC0044 RXA00863 RXA00864 RXA02843 RXN00355 F RXA00352 Contig NT Start GROO137 4758 GR00137 3469 GR00842 543 GR00613 2063 GROOO07 3458 GR00236 896 GR00236 1694 GR00842 543 WV0135 31980 GR00068 861 NT Stop Function 3496 ASPARTOKINASE ALPHA AND BETA SUBUNITS (EC 2.7.2.4) 2438 ASPARTATE-SEMIALDEHYDE DEHYDROGENASE (EC; 1.2. 1.11) 4 2,3,4.5-TETRAHYDROPYRIDINE-2CARBOXYLATE

N-SUCCINYLTRANSFERASE

(EC 2.3.1.117) 3169 SUCCINYL-DIAMINOIPIMELATE DESUCCINYLASE (EC 3.5.1.18) 4393 DIHYDRODIPICOLINATE SYNTHASE (EC 4.2.1.52) 1639 DIHYDRODIPICOLINATE REDUCTASE (EC 1.3.1.26) 2443 probable 2,3-dihydrodipicolinate N-C6-Iyase (cyclizing) (EC Corynebacterium glutamicum 4 2 3 4 ,5-TETRAHYDROPYRIDINE-2CARBOXYLATE

N.SUCCINYLTRANSFERASE

(EC 2.3.1.117) 30961 MESO-DIAMINOPIMELATE

D-DEHYDROGENASE

4 MESO-DIAMINOPIMELATE D-DEHYDROGENAS E (EC 1.4.1.16) 2006200807 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 46 RXA00972 GR00274 3 1379 DIAMINOPIMELATE DECARBOXYLASE (EC 4.1.1.20) 47 48 RXA02653 GR00752 5237 7234 DIAMINOPIMELATE DECARBOXYLASE (EC 4.1.1.20) 49 50 RXAO1 393 GR00408 4249 3380 LYSINE EXPORT REGULATOR PROTEIN 51 52 RXA00241 GR00036* 5443 6945 L-LYSINE TRANSPORT PROTEIN 53 54 RXA01 394 GR00408 4320 5018 LYSINE EXPORTER PROTEIN 56 RXA00865 GR00236 2647 3549 DIHYDRODIPICOLI NATE SYNTHASE (EC 4.2.1.52) 57 58 RXS02021 2,3.4,5-TETRAHYDROPYRIDINE-2CARBOXYLATE

N-SUCCINYLTRANSFERASE

(EC 2.3.1.117) 59 60 RXS02157 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) 61 62 RXCO0733 ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN LYSINE

METABOLISM

63 64 RXCO086i PROTEIN INVOLVED IN LYSINE METABOLISM 66 RXC00866 ZN-DEPENDENT HYDROLASE INVOLVED IN LYSINE METABOLISM 67 68 RXC02095 ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN LYSINE

METABOLISM

69 70 RXC03185 PROTEIN INVOLVED IN LYSINE METABOLISM Glutamate and glutamine metabolism Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 71 72 RXN00367 W0196 9744 14273 GLUTAMATE SYNTHASE [NADH] PRECURSOR (EC 1.4.1.14) 73 74 F RXAO007 GROO001 7107 8912 GLUTAMATE SYNTHASE (NADPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) C 76 F RXA00364 GR00074 1296 4 GLUTAMATE SYNTHASE (NADPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) 77 78 F RXA00367 GR00075 1806 964 GLUTAMATE SYNTHASE (NADPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) 79 80 RXN00076 W0154 2752 4122 GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) 81 82 F RXA00075 GROO012 2757 3419 GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) 63 84 RXN00198 W0181 7916 7368 GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) 86 F RXA00198 GROO031 2 283 GLUTAMATE SYNTHASE (NADPH)kSMALL CHAIN (EC 1.4.1.13) 87 88 RXN00365 W0196 14607 15233 GLUTAMATE SYNTHASE [NADPHJ SMALL CHAIN (EC 1.4.1.13) 69 90 F RXA00365 GR00075 630 4 GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) 91 92 RXA00366 GR00075 961 605 GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) 93 94 RXA02072 GR00628 1259 2599 NADP-SPIECIFIC GLUTAMATE DIEHYDROGENASE (EC 1.4.1.4) 96 RXA00323 GR00057 3855 5192 GLUTAMINE SYNTHETASE (EC 6.3.1.2) 97 98 RXA00335 GR00057 19180 17750 GLUTAMINESYNTHETASE (EC 6.3.1.2) 99 100 RXA00324 GR00057 5262 8396 GLUTAMATE-AMMONIA-LIGASE ADENYLYLTRANSFEASE (EC 2.7.7.42) 101 102 RXN03176 W0332 2 862 GLUTAMINASE (EC 3.5.1.2) 103 104 F RXA02879 GR10017 2 862 GLUTAMINASE (EC 3.5.1.2) 105 106 RXA00278 GR00043 2612 1581 GLUTAMINE-BINDING PROTEIN PRECURSOR 107 108 RXA00727 GR00193 614 1525 GLUTAMINE-BINDING PERIPLASMIC PROTEIN PRECURSOR 2006200807 24 Feb 2006 Table I (continued) Alanine and Aspartate and Asparagine metabolism Nucleic Acid SEQ ID NO 109 ill 113 115 117 119 121 123 125 127 129 131 Amino Acid Identiication Code SEQ ID NO 110 RXA02139 112 RXN00116 114 F RXA001 16 116 RXN0061 8 118 F RXA00618 120 F RXA00627 122 RXA02550 124 RXA02193 126 RXA02432 128 RXN03003 130 RXN00508 132 RXN00636 GROO539 W0100 GROCO018 WO0135 GROO1 63 GROO 164 GR00729 GR00645 GR00708 O01 38 WO0086 W0135 NT Start NT Stop 6739 26974 510 10288 213 854 1585 1942 2669 680 4701 20972 4901 25814 4 9182 746 1138 275 365 1695 6 5783 19944 Function ASPARAGINE SYNTHETASE (GLUTAMINE-I-YDROLYZING) (EC 6.3.5.4) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFE RSE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMMONLA-LYASE (EC 4.3.1.1) L-ASPARAGINASE (EC 3.5.1.1) ASPARTATEAMINOTRANSFEPASE (EC 2.6.1,1) ALANINE RACEMASE (EC 5.1.1.1) ALANINE RACEMASE, BIOSYNTHETIC (EC 5.1.1.1) beta-Alanine metabolism Nucleic Acid SEQ ID NO 133 135 137 Amino Acid Identification Code SEQ I D NO 134 RXA02536 136 RXS00870 138 RXS02299 ContI. NT Start NT Stop Function GR00726 8581 7828 BETA-UREIDOPROPIONASE (EC 3.5.1.6) METHYLMALONATE-SEMIALOEHYDE DEHYDROGENASE (ACYLATING) (EC 1.2.1.27) ASPARTATE 1 -DECARBOXYLASE PRECURSOR (EC 4.1.1.11) Glycine and serine metabolism Nucleic Acid SEQ ID NO 139 141 143 145 147 149 151 153 155 157 159 161 163 165 167 Amino Acid Identification Code S EQ IDNO 140 RXA01561 142 RXA01850 144 RXA00580 146 RXA01821 148 RXN02263 150 F RXA02263 152 RXA02176 154 RXN02758 156 F RXA02479 158 IFRXA02758 160 F RXA02759 162 RXA02501 164 RXN03105 166 RXS01 130 168 RXS03112 C2n!i2.

GR00435 GROO525 GROO1 56 GROO5155 W0202 GROO654 GR00641 GROD766 GR00717 GR00766 GR00766 GR00720 W0074 NT Start NT Stop Function 1113 481 7343 10253 11783 33454 11454 5082 393 5082 5330 15041 15857 2042 1827 6042 9876 12160 33813 12581 4648 4 4648 5220 13977 15423 L-SERINE OEHYDRATASE (EC 4.2.1.13) L-SERINE OEHYDRATASE (EC 4.2.1.13)' SERINE HYDROXYMETHYLTR.AN~SFERASE (EC 2.1.2.11) SARCOSINE OXIDASE (EC 1.5.3.1) SARCOSINE OXIDASE (EC 1.5.3.1) SARCOSINE OXIDASE (EC 1.5.3. 1) PHOSPHOSERINE AMINOiTRANSFERASE (EC 2.6.1.52) PHOSPHOSERINE PH-OSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATAS E (EC 3.1.3.3) PH-OSPH-OSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) SARCOSINE OXIDASE (EC 1.5.3.1) D-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) D-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) 2006200807 24 Feb 2006 Table I (continued) Threonine metabolism Nucleic Acid SEQ ID NO 169 171 173 175 177 179 181 183 Amino Acid SEQ I1D NO 170 172 174 176 178 180 182 184 Identification Code RXN00969 F RXA00974 RXA00970 RXA00330 RXN00403 F RXA00403 RXCO1 207 RXCO01 52 Contig.

WV0149 GR00274 GR00273 GROO057 WV0086 GROO088 NT Start 12053 2623 161 '12968 70041 723 NT Stop 13387 3015 1087 14410 68911 1832 Function HOMOSERINE DEHYDROGENASE (EC 1.1.1.3) HOMOSERINE DEHYDROGENASE (EC 1,1.1.3) HOMOSERINE KINASE (EC 2.7.1.39) THREONINE SYNTHASE (EC 4.2.99.2) HOMOSERINE O-ACETYLTRANSFERASE HOMOSERINE O-ACETYLTRANSFERASE (EC 2.3.1.111) CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF LYSINE AND

THREONINE

MEMBRANE ASSOCIATED PROTEIN INVOLVED IN THREONINE METABOLISM Metabolism of methionine and S-adenosyl methionine Nucleic Acid SEQ ID NO 185 187 189 191 193 195 197 199 201 203 205 207 Amino Acid SEQ I1D NO 186 188 190 192 194 196 198 200 202 204 206 208 Identification Code RXA001 15 RXN00403 F RXA00403 RXS031 58 F RXA00254 RXA02532 RXS031 59 F RXA02768 RXA00216 RXN00402 F RXA00402 RXA00405 RXA02 197 RXN02 198 .F RXA02 198 RXN03074 F RXA02906 RXN00132 F RXA00132 Contig GROO017 W0086 GRO0088 GR00038 GR00726 GR00770 GROO032 W0086 GR00088 GR00089 GRO0645 W0302 GR00646 W0042 GR 10044 W0124 GROO020 NT Start 5359 70041 723 2404 3085 1919 16286 70787 3289 NT Stop 4313 68911 1832 1811 2039 2521 15297 70188 576 3801 4025 11726 6 Function H-OMOSERINE O-ACETYLTRANSFEPASE (EC 2.3.1.31) HOMOSERINE O-ACIETYLTRANSFERASE HOMOSERINE O-ACETYLTRANSFERASE (EC 2.3.1.11) CYSTATHIONINE GAMMA-SYNTH-ASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) 5-methyttetrahydrofolate-homocysteine methyitransferase (methionine synthetase) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFH-YDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) 0-ACETYIHOMOS ERIN E SULFHYDRYLASE (EC 4.2.99.10) 1 0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) 5-METHYLTETRAHYDROFOLATE-.HOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATE..HOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATEHOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13) S-ADENOSYLMETHIONINE2DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC S-ADENOSYLMETHIONINE:2DEMETHYLMENAOUINONE METHYLTRANSFERASE (EC ADENOSYLHOMOCYSTEINASE (EC 3.3. 1.1) ADENOSYLHOMOCYSTEINASE (EC 3.3.1.1) 209 210 211 212 213 214 215 216 217 218 219 220 221 222 Z238 1741 1142 645 3612 5045 7728 7624 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 223 225 227 229 231 233 235 237 Amino Acid S§EQ ID NO 224 226 228 230 232 234 236 Identification Code F RXA01371 RXND2085 F RXA02085 F R.XA02086 RXN02648 F RXA02648 F RXA02658 RXC02238 RXC0128 Conig.

GR00398 GR00629 GR00629 GR00751 GROD752 Table 1 (continued) NT Start NT StoD Function 2339 3634 ADENOSYLHOMOCYSTEINASE (EC 3-3.1.1) 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATEHOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.14) 3496 5295 5-METHYLTETRAH-YDROPTEROYLTRIGLUTAATE.HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.14) 5252 5731 5-METHYLTETRAHYDROPTEROYLTRIGLUTMATE-HOMOCYSTEINE METHYLTRANSFE RASE (EC 2.1.1.14) 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATEHOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.14) 5254 4730 5-METHYLTETRAHYDROPTEROYLTRIGLUTAATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.14) 14764 15447 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE-HOMOCYSTEINE METHYLTRANSFERASE (C 2.1.1.14) PROTEIN INVOLVED IN METABOLISM OF S-ADENOSYLMETHIONINE,

PURINES

AND PANTOTHENATE EXPORTED PROTEIN INVOLVED IN METABOLISM OF PYRIDIMES AND

ADENOSYLHOMOCYSTEINE

238 239 240 S-adenosyl methionine (SAM) Biosynthesis Nucleic Acid Amino Acid SEQIO1DNO SEQIO1DNO 241 242 Identification Code Contig.

RXA02240 GROO654 NT Start 7160 NT Stop 8380 Function S-ADENOSYLMIETHIONINE SYNTHETASE (EC 2.5.1.6) Cysteine metabolism Nucleic Acid SEQ ID NO 243 245 247 Amino Acid SEQ -ID NO 244 246 248 Identification Code RXA00780 RXA00779 RXN00402 F RXA00402 RXS00405 RXCO01 64 Contig GR00206 GR00206 W00O86 GR00088 NT Start 1689 550 70787 NT Stop 2234 1482 70188 576 Function SERINE ACETYLTRANSFERASE (EC 2.3.1.30) CYSTEINE SYNTHASE (EC 4.2.99.8) O-ACETYLHOMOS ERIN E SULFHYDR3YLASE (EC 4.2.99.10) 0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10) 10-ACETYLS ERINE SULFHYORYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10) /0-ACETYLSERINE SULFHYORYLASE (EC 4.2.99.8) ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN CYSTEINE

METABOLISM

ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN CYSTEINE

METABOLISM

RXCO 1191 2006200807 24 Feb 2006 Table 1 (continued) Valine, leucine and isoleucine Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEGID NO 257 258 RXA02646 GR00751 3856 2568 THREONINE DEHYDRATASE BIOSYNTHETIC (EC 4.2.1.16) 259 260 RXA00766 GR00204 5091 4249 BRANCHEDCHAIN AMNOACID AMNOTANSFEASE(EC2 6 1 42 261 262 RXN01690 W0246 1296 196 BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE (EC 26.1.42) 263 264 F RXA01690 GR00473 1248 196 BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE (EC 2.6.1.42) 265 266 RXN01 026 W0143 9171 7513 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (EC 4.2.1.33) 267 268 F RXA01026 GR00294 1 1602 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (EC 4.2.1.33) 269 270 RXNO 1127 W0157 4491 3472 3-ISOPROPYLMAt.ATE DEHYDROGENASE (EC 1.1. 1.85) 271 272 F RXA01 132 GR00315 1349 1651 3-ISOPROPYLMALATE DEHYDROGENASE (EC 1.1.1.85) 273 274 RXN00536 W0219 6128 7498 2-ISOPROPYLMALATE SYNTHASE (EC 4.1.3.12) 275 276 F RXA00536 GR00137 6128 7360 2-ISOPROPYLMALATE SYNTHASE (EC 4.1.3.1) 277 278 RXN02965 W0143 7711 7121 3-SOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT (EC4.2.1.33) 279 280 RXN01929 W0127 47590 48402 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.11) DECARBOXYLASE (EC 4.1.1.44) 281 282 F RXA01929 GROD555 2766 1960 "-ETHYL-2-OXOBUTANOATE HYDROXYMETHYLTR.ANSFERASE (EC 2.1-2.11) 283 284 RXN01420 W0122 15584 14643 4-MYCAROSYL ISOVALERYL-COA TRANSFERASE (EC 285 286 RXS01 145 KETOL-ACID REDUCTOISOM ERASE (EC 1.1.1.86) 287 288 F RXAOI1145 GR00321 1075 1530 KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) Arginine and proline metabolism Enzymes of proline biosynthesis: Nucleic Acid Amino Acid Identification Code Contig NT Start NT Stop Function SEQ ID NO S1EQ I1D NO 289 290 RXA02375 GR00589 1449 223 GLUTAMATE 5-KINASE (EC 2.7.2.11) 291 292 RXN02382 W0213 5162 3867 GAMMA-GLUTAMYL. PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 293 294 F. RXA02378 GR00690 624 16 GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 295 296 F RXA02382 GR00691 2493 1894 GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 297 298 RXA02499 GR00720 11883 12692 PYRROLINE-5-CARBOXYLATE REDUCTASE (EC 1.5.1.2) 299 300 RXS02 157 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) 303 304 RXS02262 ORNITHINE CYCLODEAMINASE (EC4.3.1.12) 303 34 RXS2970ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) 305 306 F RXA01009 GR00287 4714 5943 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) 2006200807 24 Feb 2006 Table I (continued) Enzymes of proline degradation: Nucleic Acid SEQ ID NO 307 Amino Acid SEQ _IDNO, 308 Identification Code 22ntig. NT Start NT Stop Function 309 310 311 312 313 314 RXN00023 F RXA00023 F RXA02284 RXC02498 W0127 88158 64703 PROLINE DEHYDROGENASE (EC 1.5.99.8) DELTA-1 CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) GROO003 2 454 PROLINE DEHYDROGENASE (EC 1.5.99.8)/1DELTA-i.- CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) GR00660 3028 5 PROLINE DEHYDROGENASE (EC 1.5.99.8) /DELTIA-i- CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) PROTEIN INVOLVED IN PROLINE METABOLISM Synthesis of 3-Hydoxy-proline: Nucleic Acid SEQ ID NO 315 Amino Acid Identification Code Conig.

SEQ IDN NO 316 RXA01 491 GRQO42 NT Start NT Stop Function 5337 4687 DNA FOR L-PROLINE 3-HYDROXYLASE, COMPLETE CDS 3 Enzymes of ornithine, arginine and spermidine metabolism: Nucleic Acdd SEQ ID NO 317 Amino Acid S EQ 1D NO 318 Identification Code Conqjg NT Start NT Stop Function RXA02155 RXA02 156 RXN02153 F RXA02153 RXA02 154 RXA021 57 RXS02970 F RXA01009 RXA02 158 RXA02 160 RXN02 162 F RXA02161 F RXA02162 RXA02262 RXA0021 9 RXAO 1508 RXA01 757 RXA02 159 RXN02 154 RXS0147 RXS00905 RXS00906 GR00640 1913 3076 GLUTAMIATE N-ACETYLTRANSFEPRASE (EC 2.3.1.35) AMINO-ACID ACETYLTRANSFERASE (EC 2.3.1.1) GRO0640 3125 4075 ACETYLGLUTAMATE KINASE (EC 2.7.2.8) WV0122 14106 13327 N-ACETYL.GAMMA-GLUTAMYL.PHOSPHATE REDUCTASE (EC 1.2.1.38) GRO0640 757 1536 N-ACETYLGLUTAMATE5SEMIALDEHYDE

DEHYDROGENASE

GROO640 1536 1826 N-ACETYLGLUTAMATE5SEMIALDE HYD)E DEHYDROGENASE GRO0640 4079 5251 ACETYLORNITHINE AMINOTRANSFEASE (EC 2.6.1.11) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) GR00287 4714 5943 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) GR00640 5268 6224 ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) GROO640 6914 8116 ARGININOSUCCINATE SYNTHASE (EC 6.3.4.5) W0122 6583 5253 ARGINNOSUCCINATE LYASE (EC 4.3.2.11) GR00640 8180 8962 ARGININOSUCCINATE LYASE (EC 4.3.2. 1) GR00640 8949 9611 ARGININOSUCCINATE LYASE (EC 4.3.2.1) GR00654 32291 33436 ORNITHINE CYCLODEAMINASE (EC4.3.1.12) GR00032 19289 20230 SPERMIDINE SYNTHASE (EC 2.5.1.16) GR00424 12652 14190 SPERMIDINE SYNTHASE (EC 2.5.1.16) GR00498 2942 2142 PUTRESCINE OXIDASE (EC 1.4.3.10) GR00640 6231 6743 ARGININE HYOROXIMATE RESISTANCE PROTEIN W0122 13327 13037 N-ACETYL.GAMMAOGLUTAMYL-PHOSPHATE REDUCTASE (EC 1.2.1.38) CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN (EC 6.3.5.5) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 361 363 365 367 369 371 373 Amino Acid Identification Cade SEQ ID NO 362 RXS00907 364 RXS02001 366 RXS021 01 368 RXS02234 370 F RXA02234 372 RXS02565 374 RXS02937 Conlig Table 1 (continued) NT Start NT Stop Function N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) GR00654 1 3198 CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L.AMINO ACID AMIDOHYDROLASE (EC 3.5A1.14) Histidine, metabolism Nucleic Acid SEQ ID NO 375 377 379 381 383 385 387 389 391 393 Amino Acid Identification Code SEQ I1D NO 3 -76 RXAD2194 378 RXA02195 380 R.XAO1O97 382 RXA01 100 RXA0l11 RXN01 657 F R.XA0 1657 RXA0 1098 RXN01 104 F RXA0i1O4 RXN00446 F RXA00446 RXA01 105 RXA01 106 RXCO0930 RXCO1 096 R.XCO1 656 RXC0 1158 GR00645 GR00645 GR00306 GR00306 GR00306 w00lo GR00460 GR00306 W0059 GROO306 W0112 GROD1 08 GR00306 GR00306 2897 3186 4726 7072 7726 39950 2444 5499 7037 10927 24181 4 12044 13378 2055 2917 4373 6335 7094 39351 2944 4726 6432 10322 23318 525 10947 12053 NT Start NT Stop Function ATP PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.17) PHOSPHORIBOSYL-ATP PYROPHOSPHOHYDROLASE (EC 3.5.1 .31) PHOSPHORIBOSYL-AMP CYCLOHYDROLASE (EC 3.5.4.19) PHOSPHORIBOSYLFORMIMINO5-AMINOIMIDAZOLE

CARBOXAMIDE

RIBOTIDE ISOMERASE (EC 5.3.1.16) AMIDOTRANSFERASE HISH (EC AMIDOTRANSFERASE ISH (EC AMIDOTRANSFERASE HISH (EC HISF PROTEIN IMIDAZOLEGLYCEROL.PHOSPHATE DEHYDRATASE (EC 4.2.1.19) IMIDAZOLEGLYCEROL.PHOSPHATE DEHYDRATASE (EC 4.2.1.19)1 HISTIDINOL.PHOSPI-ATASE (EC 3.1.3.15) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL.PHOSPI-ATE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL.PHOSPHAjTE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL DEHYDROGENASE (EC11.1.1.23) PROTEIN INVOLVED IN HISTIDINE METrABOLISM PROTEIN INVOLVED IN HISTIDINE METABOLISM PROTEIN INVOLVED IN HISTIDINE METABOLISM MEMBRANE SPANNING PROTEIN INVOLVED IN HISTIDINE METABOLISM Metabolism of aromatic amino acids Nucleic Acid SEQ ID NO 411 413 415 417 419 421 Amino Acid Identification Code Contig.

S-EQ 1D NO 412 RXA02458 GR00712 414 RXA02790 GR00777 416 RXN00954 W0247 418 F RXA00954 GR00263 420 RXN00957 WV0208 422 F RXA00957 GR00264 NT Start NT Stop Function 4345 6948 2577 590 2764 1130 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE (EC 2.5.1.19) 4-AMIN04DEOXYCHORISMATE LYASE (EC ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.18) ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.18) ANTHRANILATE SYNTHASE COMPONENT I (EC 4.1.3.27) ANTHRANILATE SYNTHASE COMPONENT I (EC 4.1.3.27) 2006200807 24 Feb 2006 *Nucleic Acid SEQ ID NO 423 425 427 429 431 433 435 437 439 441 443 445 447 449 451 453 455 457 459 461 463 465 467 469 471 473 475 477 479 481 483 485 487 489 491 493 495 497 499 501 503 Amino Acid SEQ -IDNO 424 426 428 430 432 434 436 438 440 442 444 446 448 450 452 454 456 458 480 462 Identification Code Contig.

Table 1 (continued) NT Start NT Stop Function RXA02687 RXN01 698 F RXA01698 RXA01095 RXA00955 RXA02814 RXA0 0229 RXA02093 RXA02791 R.XA0 1699 RXA0 0952 RX N00956 F RXA00956 RXA00064 RXN00448 F RXA00448 F RXA00452 RXA00584 RXA00579 RXA00958 RXN03007 RXN02918 RXN01 116 RXNO01115 RXS001 16 F RXAOO 116 RXS00391 RXS00393 F RXA00393 RXS00446 F RXA00446 R.XS0061 8 F RXA00618 F RXA00627 RXS01 105 RXS02315 RXS02550 RXS0231 9 RXS02908 RXS 03003 RXS03026 GR00754 11306 12250 CHORISMATE MUTASE (EC 5.4.99.5) 1 PREPHENATE DEHYDRATASE (EC 4.2.1.51) W0134 11507 12736 CHORISMATE SYNTHASE (EC 4.6.1.4) GR00477 2 991 CHORISMATE SYNTHASE (EC 4.6.1.4) GR00306 3603 2821 INDOLE-3-GLYCEROL PHOSPHATE SYNTHASE (EC 4.1.1.48) GR00263 586 2007 INDOLE-3-GLYCEROL PHOSPHATE SYNTHASE (EC 4.1.1.48)1 RIBOSYL)ANTHRANI LATE ISOMERASE (EC 5.3.1.24) GR00795 598 128 ISOCHORISMATE MUTASE GR00033 1715 936 SHIKIMATE 5-DEHYDROGENASE. (EC 1.1.1.25) GR00629 12444 13247 SHIKIMATE 5-DEHYDROGENASE (EC 1.1.1.25) GR00777 6968 7795 SHIKIMATE 5-DEHYDROGENASE (EC 1. 1. 1.25) GR00477 984 1553 SHIKIMATE KINASE (EC 2.7.1.71) GR00262 97 936 TRYPTOPHAN SYNTHASE ALPHA CH-AIN (EC 4.2.1.20) W0247 1140 4 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) GR00263 2027 3157 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) GROO010 2499 3776 TYROSINEAMINOTRANSFERASE (EC 2.6.1.5) W01 12 33959 32940 PREPHENATE DEHYDROGENASE (EC 1.3.1.12) GR00109 3 668 PREPHENATE DEHYOROGENASE (EC 1.3.1.12) GROO110 854 1099 PREPHENATE DEHYDROGENASE (EC 1.3.1.12) GROO1 56 11384 10260 PHOSPHO.2-DEHYDRO.3-DEOXYHEPTONATE ALDOLASE (EC 4.1.2.15) GRO0156 5946 4087 PARA-AMINOBENZOATE SYNTHASE COMPONENT I (EC GR00264 1130 1753 PARA-AMINOBENZOATE SYNTHASE GLUTAMINE AMIDOTR.ANSFERASE COMPONENT 11 (EC 1 ANTHRANILATE SYNTHASE COMPONENT 11 (EC 4. 1.3.27) WV0208 3410 3778 ANTHRANILATE SYNTHASE COMPONENT 11 (EC 4.1.3.27) W0066 25447 25887 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) WV0182 7497 6886 3-OXOADIPATE COA-TRANSFERASE SUBUNIT B (EC 2.8.3.6) W0182 10347 11099 3-OXOADIPATE ENOL-LACTONE HYDROLASE (EC 3.1.1.24) /4-

CARBOXYMUCONOLACTONE

ASPARTATE AMINOTRANSFERASE EC 1.1) GROO18 510 4 ASPARTATE AMINOTRANSFERASE (EC. 2.6. 1.1) O-SUCCINYLBENZOIC ACID..COA LIGASE (EC 6.2.1.26) 1.4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC GR00086 4030 4911 1,4-DIHYDROXY-2-NAPHTHOATE 0OCTAPRENYLTRANSFE RASE (EC HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) GROO108 4 525 HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) GR00163 213 746 ASPARTATE AMINOTRANSFERASE (EC 2.6. 1. 1) GR00164 854 1138 ASPARTATE AMINOTRANSFERASE (EC 2.6.1. 1) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) 2 -SUCCINYL-6.HYDROXY24-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE I 2-OXOGLUTARATE DECARBOXYL.ASE (EC 4.1.1.71) ASPARTATE AMINOTRANSFERASE (EC 2'.6.1.1) NAPHTHOATE SYNTHASE (EC 4.1.3.36) O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) ASPARTATE AMINOTRANSFERASE (SC 2.6.1.1) 3-DEHYDROQUINATE DEHYDRATASE (EC 4.2. 1. 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 505 Amino Acid SEQ0 I D NO 506 507 508 509 510 511 512 513 514 Identification Code Contig.

RXS03074 RXCO1434 RXC02080 RXC02789 RXC02295 Table I (continued) NT Start INT Stop Function S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS AND RIBOFLAVIN MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO

ACIDS

MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS Aminobutyrate metabolism Nucleic Acid SEQ ID NO 515 517 519 Amino Acid SJEQ I1D NO 516 518 520 Identification Code RXN03063 RXN02970 F RXAO 1009 W0035 W00O21 GROD287 NT Start 666 4714 4714 INT Sto- 1697 6081 5943 Function 4-amninobutyrate aminotransferase (EC 2.6.1.19) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) Vitamins, vitamin-like substances (cofactors), nutraceuticals Thiamine metabolism Nucleic Acid SEQ ID NO 521 523 525 527 529 531 533 535 537 539 541 543 545 547 549 551 Amino Acid S EQ 1D NO 522 524 526 528 530 532 534 536 538 540 542 544 546 548 550 552 Identification Code RX.AO1551 RXA01019 RXA01 352 RXA0 1381 RXA01 360 RXAO1 361 RXA01 208 RXA00838 RXA02400 RXN01 209 F RXA01209 RXN01413 RXN01617 F RXA01617 RXS01 807 RXCO1021 Co2t!!1 GR00431 GROD291 GR00393 GROD403 GROD394 GR00394 GR00348 GR00227 GR00699 W0270 GR00348 wo0050 wo0050 GR00451 NT Start NT Stop Function 2945 6 609 3206 162 963 229 1532 1988 1019 1019 27306 22187 2 4819 995 4 2286 4 378 1032 633 2557 2446 2446 27905 22858 616 THIAMIN BIOSYNTHESIS PROTEIN THI .C THIAMIN-MONOPHOSPHATE KINASE (EC 2.7.4.16) THIAMIN-PHOSPHATE PYROPHOSPHORYLASE (EC 2.5.1.3) THIF PROTEIN THIG PROTEIN THIG PROTEIN HYDROXYETHYLTHIAZOLE KINASE (EC 2.7.1.50) APBA PROTEIN THIAMIN BIOSYNTHESIS PROTEIN X PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PYRIDOXINE KINASE (EC 2.7.1.35) CYTOSOLIC KINASE INVOLVED IN METABOLISM OF SUGARS AND THIAMIN 2006200807 24 Feb 2006 Table I (continued) Riboflavin metabolism Nucleic Acid SEQ ID NO 553 555 557 559 561 563 565 567 569 571 573 575 577 579 581 583 585 587 589 591 593 Amino Acid S9EQ ID NO 54 556 558 560 562 564 566 568 570 572 574 Identification Code RXN02246 F RXA02246 RXA02247 RXN02248 F RXA02248 RXN02249 F RXA02249 RXA02250 RXAO 1489 RXA021 35 RXA01489 RXN01 712 F R.XA0 1712 RXN02384 RXN01560 RXN00667 RXCO1711 RXC02380 F RXA02380 RXC02921 RXCO1 434 Conig NT Start NT Stop Function W0130 4388 5371 diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26)1/5-amino- 6 -(5-phosphodbosylamino)uracil reductase (EC 1.1.1.193) GROO654 14299 15282 RIBG PROTEIN riboflavin-specific deaminase GROO654 15286 15918 RIBOFLAVIN SYNTHASE ALPHA CHAIN (EC 2.5.1.9) W0130 6021 7286 GTP CYCLOHYDROLASE 11 (EC 3.5.4.25) 3.4-DIHYDROXY-2-BUTANONE 4- PHOSPHATE SYNTHASE GR00654 15932 17197 RIBA PROTEIN GTP cyclohydrolase 11 [EC:3.5.4.25) W0130 7301 7777 6,7-DIMETHYL.8-RIBITYLLUMAZINE SYNTHASE (EC 2.5.1.9) GR00654 17212 17688 RIBH PROTEIN 6,7-dimethyl-8-ribityllumazine synthase (dmrl synthase, lumazine synthase, riboflavin synthase beta chain) IEC:2.5.1.9] GR00654 17778 18356 RIBX PROTEIN GR00423 3410 2388 RIBOFLAVIN KINASE (EC 2.7.1.26) FMN ADENYLYLTR.ANSFERASE

(EC

2.7.7.2) GR00639 2809 1736 NICOTINATENUCLEOTIDE-.DIMETHYLBENZIMIDAZOLE PHOSPHORIBOSYLTRANSFEPASE (EC 2.4.2.21) GR00423 3410 2388 RIBOFLAVIN KINASE (EC 2.7.1.26) t FMN ADENYLYLTRANSFERASE

(EC

2.7.7.2) W0l 91 8993 8298 RIBOFLAVIN-SPECIFIC DEAMINASE (EC GR00484 2652 2152 RIBOFLAVIN-SPECIFIC DEAMINASE (EC W0213 1386 679 ALPHA-RIBAZ-OLE-5'-PHOSPHATE PHOSPHATASE (EC WV0319 767 438 RIBOFLAVIN-SPECIFIC DEAMINASE (EC W0109 1363 350 DRAP DEAMINASE MEMBRANE SPANNING PROTEIN INVOLVED IN RIBOFLAVIN METABOLISM PROTEIN INVOLVED IN RIBOFLAVIN METABOLISM GR00691 709 56 Predicted nucleotidyltransferases CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF RIBOFLAVIN AND

LIPIDS

MEMBRANE SPANNING PROTEIN IN VOLVED IN METABOLISM OF AROMATIC AMINO ACIDS AND RIBOFLAVIN I 576 578 580 582 584 586 588 590 592 594 Vitamin B36 metabolism Nucleic Acid Amino Acid SEQ ID NO S EQIDNO 595 596 Identification Code Cog.

RXA01807 GR00509 NT Star NT Stop 7868 7077 Function PYRIDOXINE KINASE (EC 2.7.1.35), pyridoxal/pyridoxine,'pyridoxa mine kinase 2006200807 24 Feb 2006 Table 1 (continued) Nicotinate (nicotinic acid), nicotinamide, NAD and NADP Nucleic Acdd SEQ ID NO 597 599 601 603 Amino Acid Identification Code S-EQ ID NO 598 RXN02754 600 F RXAO2405 602 F RXA02754 604 RXA02112 225aig NT Start NT Stop Function W00154 22564 GROO701 774 GR00766 3 GRO0632 5600 GR00632 4310 23901 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) 4 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) 488 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) 6436 NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE (CARBOXYLATING) (EC 2.4.2.19) 5593 QUINOLINATE SYNTHETASE A 605 606 RXA021 11 NAD Biosynthesis Nucleic Acid Amino Acid Identification Code Contig SEQ ID NO SIEQ ID NO 607 608 RXA01073 GR0O30 609 610 RXN02754 WV0084 NT Start NT Stop Function 10 1274 22564 2104 NH(3)-DEPENOENT SYNTHETASE (EC 6.3.5.1) 23901 NICOTINATE PHOSPHORIBOSYLTRANSFEPASE (EC 2.4.2.11) Pantothenate and Coenzyme A (CoA) biosynthesis Nucleic Acid SEQ ID NO 611 613 615 Amino Acid Identification Code 222tia: S EQ I1D NO 612 RXA02299 GROO66 614 RXA01 928 GR0055 616 RXN01929 W0127 NT Start NT Stop Function i2 10452 5S 1957 47590 F RXA01929 RXA01521 RXS01 145 F RXAO1 145 RXA02239 RXA00581 RXS00838 RXC02238 GR00555 2766 GR00424 25167 GR00321 1075 GR00654 57814 GROO156 7572 10859 ASPARTATE 1 -DECARBOXYLASE PRECURSOR (EC 4.1.1.11) 1121 PANTOATE-BETA-ALANINE LIGASE (EC 6.3.2-1) 48402 3-METHYL-2.OXOBUTANOATE HYDRQXYMETHYLTRANSFERASE (EC 2.1.2.11) DECARBOXYLASE (EC 4.1.1.44) 1960 3-METHYL-2.OXOBUTANOATE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.11) 25964 PANTOATE-BETAALANINE LIGASE (Et 6.3.2.1) KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) 1530 KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) 7049 DNAIPANTOTHENATE METABOLISM FIZAVOPROTEIN 8540 PANTOTHENATE KINASE (EC 2.7.1.33) 2-DEHYDROPANTOATE 2-REDUCTASE (EC 1.1.1.169) PROTEIN INVOLVED IN METABOLISM OF S-ADENOSYLMETHIONINE,

PURINES

AND PANTOTHENATE Biotin metabolism Nucleic Acid Amino Acid Identific-ation Code Co~nti SEQ ID NO SEQ -10No 633- 634 RXN03058 WV0028 NT Start NT Stop Function 8272 8754 BIOTIN SYNTHESIS PROTEIN BlOC 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 635 637 639 Amino Acid SEQ ID NO 636 638 640 Identification Code Conig Table 1 (continued) NT Start NT S-top Function F RXA02903 RXA001 66 RXA00633 RXA00632 RXA00295 RXA00223 RXN00262 F RXA00262 RXN00435 F RXA00435 F RXA02801 RXA02516 RXA02517 GR10040 GROO025 GROO1 66 GRO 1 66 GROO047 GRO0032 W01 23 GRO0040 W0112 GROW 00 GR00782 GR00723 GR00723 11532 3650 3556 2281 3407 23967 16681 79 10037 3563 438 1724 2989 12014 4309 2288 1610 4408 22879 15608 897 11209 2949 4 2986 3435 BIOTIN SYNTHESIS PROTEIN BlOC BIOTIN SYNTHESIS PROTEIN BlOC ADNSLEHOIE8AIO7OOOAOT

AMINOTRANSFERASE

(EC 2.6.1.62) DETHIOBIOTIN SYNTHETASE (EC 6.3.3.3) BIOTIN SYNTHASE (EC 2.8.1.6) NIFS PROTEIN NIFS PROTEIN NIPS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFU PROTEIN Lipoic Acid Nucleic Acid SEQ 0 NO 661 663 665 667 Amino Acid SEQ 10-NO 662 664 666 668 Identification Code RXA01747 RXA01 746 RXA02106 RXS0 1183 Contig.

GR00495 GR00495 GR00632 NT Start NT Stop Function 2506 3549 LIPOIC ACID SYNTHETASE 1614 2366 LIPOATE-PROTEIN LIGASE B (EC 472 1527 LIPOATE-PROTEIN LIGASE A (EC DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT (E2) OF 2.

OXOGLUTARATE DEHYDROGENASE COMPLEX (EC 2.3.1.61) LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) LIPOAMIDE DEHYOROGENASE COMPONENT (E3) OF BRANCHED-CHAIN AIPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) 669 670 RXS01260 672 RXS01261 Folate biosynthesis Nucleic Acid S EQ I D NO 6'7 3 675 677 679 681 683 685 Amino Acid S§EQ 1D NO 674 676 678 680 682 684 686 Identification Code Conhig NT Start NT Stop Function RXA02717 RXN02027 *F RXA02027 RXAOO 106 RXN01 321 F RXA01 321 RXA00461 GR00758 WV0296 GR00616 GROO014 W0082 GR00384 GRO01 16 GR00424 GR00424 18281 503 500 17469 8868 23 428 20922 22360 17400 1003 6 17924 9788 559 1279 21509 22749 6, l0-N4ETHYLENETETRAH.YDROFOLATE REDUCTASE (EC 1.7.99.5) 5.FORMYLTETRAHYDROFOLATE CYCLO-LIGASE (EC 6.3.3.2) 5-FORIMYLTETRAHYDROFOLATE CYCLO-LIGASE (EC 6.3.3.2) DIHYDROFOLATE REOUCTASE (EC 1.5.1.3) FORMYLTETRAHYOROFOLATE DEFORMYLASE (EC 3.5.1.10) FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) METI-YLENETETRAHYDROFOLATE DEHYOROGENASE (EC 1.5.1.5)1 METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE (EC 3.5.4.9) GTP CYCLOHYDROLASE I (EC 3.5.4.16) DIHYDRONEOPTERIN ALDOLASE (EC 4.1.2.25) 687 688 RXAOI1514 6.89 690 RXAO 1516 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 691 693 695 697 699 701 703 Amino Acid SEQ -ID NO 692 694 696 698 700 702 704 Identification Code RXA01515 RXA02024 RXA00106 RXA00989 RXAO1517 RXA00579 RXA00958 RXA02790 RXA00106 RXN02198 F RXA02198 RXN02085 F RXA02085 F RXA02086 RXN02648 F RXA02648 F RXA02658 RXS02197 RXC00988 RXCO1518 RXCO1942 Metabolism Contig.

GR00424 GR00613 GR00014 GR00280 GR00424 GR00156 GR00264 GR00777 GROO014 W0302 GR00646 W0126 GR00629 GR00629 GR00751 GR00752 21513 4026 17469 2903 22752 5946 1130 5806 17469 9228 2483 8483 3496 5252 22364 4784 17924 1371 23228 4087 1753 6948 17924 11726 6 10717 5295 5731 4730 15447 DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) DIHYDROFOLATE REDUCTASE (EC 1.5.1.3) FOLYLPOLYGLUTAMATE SYNTHASE (EC 6.3.2.17) 2-AMINO-4-HYDROXY-6-HYDROXYMETHYLDIHYDROPTERIDINE PYROPHOSPHOKINASE (EC 2.7.6.3) PARA-AMINOBENZOATE SYNTHASE COMPONENT I (EC PARA-AMINOBENZOATE SYNTHASE GLUTAMINE AMIDOTRANSFERASE COMPONENT II (EC /ANT-RANILATE SYNTHASE COMPONENT II (EC 4.1.3.27) 4-AMINO-4-DEOXYCHORISMATE LYASE (EC DIHYDROFOLATE REDUCTASE (EC 1.5.1.3) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATE-.HOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13)

METHYLTRANSFERASE

5-METHYLTETRAHYDROTEROYLTRIGLUTAMA

TEHOMOCYSTEINE

METHYLTRANSFERASE (SC 2.1.1.14) METHYLTRANSFERASE (SC 2.1.1.14) METHYLTRANSFERASE (SC 2.1.1.14) METHYLTRANSFERASE (SC 2.1.1.14) METHYLTRANSFERASE (SC 2.1.1.14) 5-METHYLTETRAHYDROFOLATS.HOMOCYSTEINE

METHYLTRANSFERASE

(EC 2.1.1.13) PROTEIN INVOLVED IN FOLATS METABOLISM MEMBRANE SPANNING PROTEIN INVOLVED IN FOLATE METABOLISM ATP-BINDING PROTEIN INVOLVED IN FOLATS METABOLISM Table I (continued) NT Start NT StoD Function 5254 14764 Molybdopterin Nucleic Acid SEQ ID NO 733 735 737 739 741 743 745 747 Amino Acid SEQ ID NO 734 736 738 740 742 744 746 748 Identification Code *RXN02802 F RXA02B02 F RXA00438 RXN00437 F RXA00437 RXN00439 F RXA00439 F RXA00442 Contig.

W0112 GR00783 GROO103 W01 12 GRO0103 W0112 GROO104 GROO105 NT Start 17369 7 362 17824 3 18742 2 830 NT Stop 16299 474 796 17369 362 18275 196 1087 Function MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN (MPT) CONVERTING FACTOR, SUBUNIT 2 MOLYBDOPTERIN (MPT) CONVERTING FACTOR, SUBUNIT 2 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 749 751 753 755 Amino Acid STEQ I1D NO 750 752 754 756 Identification Code RXA00440 RXN00.441 F RXA00441 RXN02085 Contig Table I (continued) NT Start NT Stop Function 758 759 760 761 762 763 764 765 766 F RXA02085 F RXA02086 RXN02648 F RXA02648 F RXA02658 RXA01516 RXA01515 RXA02024 RX.A0 1719 R.XA01 720 RXS 03223 F RXA01970 RXA02629 RXA02318 RXAO 1517 RXN01 304 RXS 02556 RXS02560 GROO104 196 654 MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN CB W01 12 19942 18779 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN GROO105 2 793 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE..HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.14) GR00629 3496 5295 METHYLTRANSFERASE (EC 2.1.1.14) GR00629 5252 5731 METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) GR00751 5254 4730 METHYLTRANSFERASE (EC 2.1.1.14) GR00752 14764 15447 METHYLTRANSFERASE (EC 2.1.1.14) GR00424 22360 22749 DIHYDRONEOPTERIN ALDOLASE (EC 4.1.2.25) GR00424 21513 22364 DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) GR00613 4026 4784 DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) GR00488 1264 704 MOLYBDOPTERIN-GLIANINE DINUCLEOTIDE BIOSYNTHESIS PROTEIN A GR00488 2476 1268 MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN GR00568 2 1207 MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN GR00748 1274 690 MOLYBDOPTERIN BIOSYNTHESIS CNX1 PROTEIN GR00665 9684 9962 (D90909) pterin-4a -ca rbinola mine dehydratase [Synechocystis sp.] GRO0424 22752 23228 2 -AMINO-4-HYDROXY-6.HYDROXYMETHYLDIHYDROPTERIDINE PYROPHOSPHOKINASE (EC 2.7.6.3) W0148 4449 4934 MOLYBDOPTERIN BIOSYNTHESIS MOG PROTEIN FLAVOHEMOPROTEIN DIHYDROPTERIDINE REDUCTASE (EC 1.6.99.7) OXYGEN-INSENSITIVE NAD(P)H NITROREDUCTASE (EC1.-.I DIHYDROPTERIDINE REDUCTASE (Eti .6.99.7) Vitamin 1312, porphyrins and heme metabolism Nucleic Acid SEQ ID NO 793 795 797 799 801 803 805 807 809 811 Amino Acid S EQ 1DNO 794 796 798 800 802 804 806 808 810 812 Identification Code ontig NT Start NT Stop Function RXA00382 RXAOO 156 RXA00624 RXA00306 RXA00884 RXN02503 F RXA02503 RXA00377 RXN02504 F RXA02504 GROO082 GR00023 GR00163 GROO051 GR00242 W0007 GR00720 GROO081 W0007 GR00720 2752 10509 7910 2206 10137 22456 16906 1427 22805 17379 1451 9400 8596 1274 11276 22854 17340 306 23362 17816 GLUTAMATE-I-S EMIALDEHYDE 2,1 -AMINOMUTASE (EC 5.4.3.8) FERROCHELATASE (EC 4.99.1.1) FERROCHELATASE (EC 4.99.1.1) HEMK PROTEIN OXYGEN-INDEPENDENT COPROPORPHYRINOGEN III OXIDASE (EC PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) PORPHOBILINOGEN DEAtMINASE (EC 4.3.1.8) UROPORPHYRINOGEN DECARBOXYLASE (EC 4.1.1.37) PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) 2006200807 24 Feb 2006 Nucleic Acid SEQ ID NO 813 815 817 819 Amino Acid SEQ ID NO 814 816 818 820 Identification Code RXNO1 162 F RXAO1 162 RXA01 692 RXN00371 823 824 F RXAC0371 F RXAC0374 RXN00383 F RXA00376 F RXA00383 RXA01 253 RXA021 34 RXA021 35 RXA021 36 RXN031 14 RXN01 810 RXS03205 F RXA00306 RXCO 1715 Conti.

w0088 GR00330 GR00474 W0226 GRO0078 GRO0079 W0223 GROO81 GR00082 GR00365 GR00639 GROO639 GR00639 wV0088 WV0082 524 4 749 5973 6 371 2863 6 2863 1787 801 1736 2841 552 663 Table 1 (continued) NT Start NT Stp Function PRECORRIN-6Y METHYLASE (EC 2. 1. PRECORRIN-6Y METHYLASE (EC 2. UROPORPHYRIN-111 C-METHYLTRANSFERASE (EC 2.1.1.107) UROPORPHYRIN-111 C-METHYLTRANSFE RASE (EC 2.1.1.107)1 UROPORPHYRINOGEN-111 SYNTHASE (EC 4.2.1.75) UROPORPHYRIN-11 0-METHYLTRANSFERASE (EC 2.1.1.107)1 UROPORPHYRINOGEN-111 SYNTHASE (EC 4.2.1.75) UROPORPHYRIN-111 C-METHYLTRANSFERASE (EC 2.1.1.107)1 UROPORPHYRINOGEN-111 SYNTHASE (EC 4.2.1.75) PROTOPORPHYRINOGEN OXIDASE (EC 1.3.3.4) PROTOPORPHYRINOGEN OXIDASE (EC 1.3.3.4) PROTOPORPHYRINOGEN OXIDASE (EC 1.3.3.4) COBYRIC ACID SYNTHASE COBALAMIN (5'-PHOSPHATE) SYNTHASE NICOTINATENUCLEOTIE-DIMEHYLBENZIID70LE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.21) COBINAMIDE KINASE I COBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE COBG PROTEIN (EC HEMIN-BINDING PERIPLASMIC PROTEIN HMUT PRECURSOR HEMK PROTEIN HEMK PROTEIN CYTOSOLIC PROTEIN INVOLVED IN PORPHYRIN METABOLISM Vitamin C precursors Nucleic Acid SEQ ID NO 849 851 853 855 857 859 861 863 865 867 Amino Acid SEQ ID NO 850- 852 854 856 858 860 862 864 866 868 Identification Code RXN00420 F RXA00420 F RXA00426 RXN00708 F R.XA00708 RXA02373 RXS00389 RXS0041 9 RXCO0416 RXC02206 WV01 12 GR00096 GR00097 GROO1 85 GR00688 NT Start NT Stop Function 2511 1048 1-GULONOLACTONE OXIDASE (EC 1.1.3.8) 2 541 L-GULONOLACTONE OXIDASE (EC4~.1.3.8) 1737 2258 L-GULONOLACTONE OXIDASE (EC 1.0..8) 4678 3872 2,5-DIKETO-D.GLUCONIC ACID REDUCTASE (EC 1. 2030 1359 2,5-DIKETO.D-GLUCONIC ACID REDUCTASE (EC 111- 1540 626 2 ,5-OIKETO.D-GLUCONIC ACID REDUCTASE (EC 1. 1. oxoglutarate semialdehyde dehydrogenase (EC 1.2. ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) MEMBRANE SPANNING PROTEININVOLVED IN METABOLISM OF VITAMIN C, PRECURSORS OXIDOREDUCTASE INVOLVED IN METABOLISM OF VITAMIN C PRECURSORS Vitamin K2 Nucleic Acid SEQ ID NO 869 Amino Acid S-EQ -ID NO 870 Identification Code Contig RXS03074 NT Start NT Stop Function S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC 2006200807 24 Feb 2006 Nucleic Acid Amino Acid SEQ ID NO SEQ0 IDNO 871 872 873 874 Identification Code F RXA02906 RXA02315 RYA0231 9 RXS00393 F RXA00393 RXA00391 RXS02908 Cont GR10044 GR00565 GR00665 GROO086 GROO086 Table I (continued) NT Start NT Stop Function 1142 645 S-ADENOSYLMETHIONINE:2-DEMETHY1MENAQUINONE METHYLTRANSFERASE (EC 8011 6383 2-SUCCINYL-6.-HYDROXY-2,4-CYCLOHEXADIENE1

-CARBOXYLATE

SYNTHASE 12-OXOGLUTARATE DECARBOXYLASE (EC 4.1.1.71) 9977 10933 NAPHTHOATE SYNTHASE (EC 4.1.3.36) 1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC 4030 4911 1 ,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFEPRASE (EC 2031 2750 O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1,26) Ubiquinone biosynthesis Nucleic Acid SEQ I0 NO 885 887 889 891 893 Amino Acid SE Q IDN NO i86 888 890 892 894 Identification Code RXA00997 RXA021 89 RXA0231 1 RXN0291 2 RXS00998 C222ag GR00283 GROO642 GROO665 W0135 NT Start 2389 986 3073 13299 NT Stop 18D8 249 2384 12547 Function 3-DEMETHYLUBIQUINONE-9 3-METHYLTRANSFERASE (EC 2.1.1.64) 3-DEMETHYLUBIQUINONE-9 3-METHYLTRANSFERASE (EC 2.1.1.64) 3-DEMETHYLUBIQUINONE-9 3-METHYLTRANSFERASE (EC 2.1.1.64) UBIQUI NONEIMENAQUI NONE BIOSYNTHESIS METHLYTRANSFERASE

UBIE

(EC COMA OPERON PROTEIN 2 Purines and Pyrimidines and other Nucleotides Regulation of purine and pyrimidine biosynthesis pathways Purine metabolism Purine Biosynthesis Nucleic Acid SEQ ID NO 89g 5 897 899 901 903 905 907 Amino Acid SJEQ 1D NO 896 898 900 902 904 906 908 Identification Code R.XA 1 215 RXN00558 F RXA00558 RXN00626 F RXA00629 F RXA00626 RXA02623 Coig. NT Start NT StoF Function GR00352 1187 213 RIBOSE-PHOSPH-ATE PYROPHOSPHOKINASE, PRPP syntlietase (EC 2.7.6. 1) W0l 03 8235 9581 AMIDOPH0SPHORIBOSYLTRANSFERASE (EC, 2.4.2.14) GROOl 48 61 501 AMIDOPHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.14) W0135 11624 10362 PHOSPHORIBOSYLAMINE..GLYCINE LIGASE (EC 6.3.4.13) GROO165 1450 1713 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (EC 6.3.4.13) GR00164 1 780 PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, GARS (EC 6.3.4.13) GR00746 4875 4285 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (EC 6.3.4.13)1/ PHOSPHORIBOSYLFORMYLGLYCINAIDINE CYCLO-LIGASE (EC 6.3.3.1)1 PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE (EC 2.1.2.2) GR00418 10277 9054 PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE 2 (EC 909 910 RXA01442 2006200807 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stoo Function SEQ ID NO SEQ ID NO 911 912 RXN00537 VV0103 3351 5636 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) 913 914 F RXA02805 GR00786 54 638 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) 915 916 F RXA00537 GR00138 23 697 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) 917 918 F RXA00561 GROO150 2 280 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) 919 920 RXA00541 GROO139 2269 2937 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) 921 922 RXA00620 GROO1 63 3049 3939 PHOSPHORIBOSYLAMIDOIMIDAZOLE-SUCCINOCARBOXAMIDE

SYNTHASE

(EC 6.3.2.6) 923 924 RXN00770 VV0103 9514 10783 PHOSPH-ORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1) 925 926 IF RX.A00557 GR00147 15 818 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1) 927 928 F RXA00770 CR00204 7809 7495 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1) 929 930 RXN02345 VV0078 4788 5984 PHOSPH-ORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT (EC 4.1.1.21) 931 932 F RXA02345 GR00676 1534 725 PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT (EC 4.1.1.21) 933 934 RXN02350 WV0078 8369 8863 PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (C 4.1.1.2 1) 935 936 F RXA02346 CR00677 127 5 PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (C 4.1.1.21) 937 938 F RXA02350 GR00678 1120 911 PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (EC 4.1.1.21) 939 940 RXA01087 CR00304 498 1373 PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE (EC 4.1.1.21) 941 942 RXA00619 GR00163 793 2220 ADENYLOSUCCINATE LYASE (EC 4.3.22) 943 944 RXA02622 CR00746 4274 2715 PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFE RASE (EC IMP CYCLOHYDROLASE (EC 3.5.4.10) GMP, GDP, AMP and ADP synthesis, from inosine-5'-monophosphate

(IMP)

Nucleic Acid Amino Acid Identification Code Contig. NT Start NT StoD Function SEQ I0 NO S EQ ID NO 945 946 RXN00488 VV0086 19066 20583 INOSINE.5'.MONOPHOSPHATE DEI-YiRp'OGENASE (EC 1.1.1.205) 947 948 IF RXA00492 CR00122 1171 1644 INOSINE-5'.MONOPHOSPI{ATE DEHYbROGENASE (EC 1.1.1.205) 949 950 F RXA00488 CR00121 1 534 INOSINE.5.-MONDPHOSPHATE.DEHYDROGENASE (EC 1.1.1.205) 951 952 RXA02469 CR00715 1927 497 INOSINE-5'-MONOPHOSPHATE DEHYQROGENASE (C 1.1.1.205) 953 954 RXN00487 WV0086 23734 25302 CMP SYNTHASE (GLUTAMINE-HYDROLYZINC] (EC 6.3.5.2) 955 956 F RXA00487 CR00120 712 2097 CMP SYNTHASE (EC 6.3.4. 1) 957 958 RXA02237 CR00654 4577 5146 GUANYLATE KINASE (EC 2.7.4.8) 959 960 RXA0 1446 CR00418 17765 16476 ADENYLOSUCCINATE SYNTHETASE (EC 6.3.4.4) 961 962 RXA00619 CR00163 793 2220 ADENYLOSUCCINATE LYASE (EC 4.3.2.2) 963 964 RXA0068B CR00179 10443 10985 ADENYLATE KINASE (EC 2.7.4.3) 965 966 RXA00265 CR00040 3769 3362 NUCLEOSIDE DIPHOSPHATE KINASE (EC 2.7.4.6) 2006200807 24 Feb 2006 Table I (continued) GMPIAMP degrading activities Nucleic Acid Amino Acid Identification Code Cni. NT Start NT Stop Function SEQ ID NO IEQID NO 967 968 RXA00489 GROO121 654 1775 GMP REDUCTASE (EC 1.6.6.8) 969 970 RXN02281 W0152 1693 3323 AMP NUCLEOSIDASE (EC 3.2.2.4) 971 972 F RXA02281 GR00659 1101 34 AMP NUCLEOSIDASE (EC 3.2.2.4) Pyrimidine metabolism Pyrimidine biosynthesis de novo: Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQID NO 973 974 RXA00147 975 976 RXA00145 977 978 RXAOO 146 979 980 RXA0220B 981 982 RXA01660 983 984 RXA02235 985 986 RXN01892 987 988 IF RXA01892 989 990 RXA00105 991 992 RXAOO 131 993 994 RXA00266 995 995 RXA00718 997 998 RXA01599 999 1000 RXN02234 1001 1002 F RXA02234 1003 1004 RXN00450 1005 1006 F RXA00450 1007 1008 RXN02272 1009 1010 F RXA02272 1011 1012 RXN03004 1013 1014 RXN03137 1015 1016 RXN03171 1017 1018 F RXA02857 oni. NT Start NT Stop Function GR00022 9722 10900 CARBAMOYL-PHOSpHATE SYNTHASE SMALL CHAIN (EC 6.3.5.5) GR00022 7258 8193 ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN (EC 2.1.3.2) GR00022 8249 9589 DIHYIDROOROTASE (EC 3.5.2.3) GR00647 2 1003 DIHYIDROOROTATE DEHYDROGENASE (EC 1.3.3.1) GR00462 591 1142 OROTATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2. GR00654 3207 4040 OROTIDINE 5'-PHOSPHATE DECARBOXYL.ASE (EC 4.1.1.23) W01 50 3020 3748 URIDYLATE KINASE (EC GR00542 47 775 URIDYLATE KINASE (EC GROO014 16672 17346 THYMIDYLATE SYNTHASE (EC 2.1.1.45) GROO020 7621 7013 THYMIDYLATE KINASE (EC 2.7.4.9) GROO040 3769 3362 NUJCLEOSIDE DIPHOSPHATE KINASE (EC 2.7.4.6) GR00188 4576 5283 CYTIDYLATE KINASE (EC 2.7.4.14).

GR00447 8780 10441 CTP SYNTHASE (EC 6.3.4.2) W0134 24708 28046 CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) GR00654 1 3198 CARBAMOYL-PHOSPHATE SYNTHXtOSE* LARGE CHAIN (EC 6.3.5.5) W0112 34491 34814 CYTOSINEDEAMINASE(EC3541) GROO110 322 5 CYTOSINE DEAMINASE (EC 3.5.4.1).

W0020 15566 16810 CYTOSINE DEAMINASE (EC35.4.1).

GR00655 6691 7935 CREATININE DEAMINASE (EC 3.5.4.21).

W0237 1862 2341 DEOXYCYTIDINE TRIPHOSPHATE DEAMINASE (EC 3.5.4.13) WV0129 9680 9579 THYMIDYLATE SYNTHASE (EC 2.1.1.45) W0328 568 1080 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) GRIO003 570 1082 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) 2006200807 24 Feb 2006 Table I (continued) Purine and pyrimidine base, nucleoside and nucleotide salvage, interconversion, reduction and degradation: Purines: Nucleic Acid SEQ 10 NO 1019 1021 1023 1025 1027 1029 1031 1033 1035 1037 1039 1041 1043 1045 1047 1049 1051 1053 1055 Amino Acid SE Q I1D NO 1020 1022 1024 1026 1028 1030 1032 1034 1036 1038 1040 1042 1044 1046 1048 1050 1052 1054 1056 Identification Code RXA02771 RXA01512 RXA0203 1 RXA00981 RXN02772 F RXA02772 F RXA02773 RXAO 1835 RXA01 483 RXNO01027 F RXA01024 F RXA01027 RXA01528 RXA00072 RXA01 878 RXN02281 F RXA02281 RXN01 240 RXN02008 Co2a&g GR00772 GR00424 GR00618 GR00276 W01 71 GR00772 GR00772 GROO51 7 GR00422 W01 43 GR00293 GR00294 GRO0425 GROO012 GR00537 W0152 GR00659 Woogo0 VV01 71 Contig.

WV0120 GR00557 GR00731 GR00720 W0084 GROO301 GR00302 W0084 GROO550 GROO301 GR00237 GR00413 GROO423 NT Start 1329 17633 3820 3388 2045 1962 2741 3147 19511 5761 661 2580 5653 446 1239 1893 1101 30442 1138 NT Start 10268 3 5418 10059 38084 693 3402 32843 1321 1240 2 660 NT Stop 1883 18232 3347 4017 1011 2741 2902 3677 18240 6768 5 2347 5126 6 2117 3323 34 29420 5 Function ADENINE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.7) HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.8) XANTHINE-GUANINE PI-OsPHORIBOSYLTRANSFEASIE (EC 2.4.2.22) GTP PYROPHOSPHOKINASE (EC 2.7.6.5) GUANOSINE.3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE

(EC

3.1.7.2) GUANOSINE-3,5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE

(EC

3.1.7.2) GUANOSINE-3.5'-BIS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE

(EC

3.1.7.2) GUANOSINE-3,5'-BIS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE

(EC

3.1.7.2) DEOXYGUANOSINETRIPHOSPHATE TRIPHOSPHOHYDROLASE (EC 3.1.5. 1) DIADENOSINE 5',5-P,P4-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.17) IDIADENOSINE 1,P4-TETRAPHOSP HATE HYDROLASE (EC 3.6.1.17) DIADENOSINE 5,5"'-P1P4-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.17) DIADENOSINE 5,5"'-P1,P4-TETRAPHOSP{ATE HYDROLASE (EC 3.6.1.17) PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE (EC 1.8.99.4) DIMETHYLADENOSINE TRANSFERASE (EC 2.1. AMP NUCLEOSIDASE (EC 3.2.2.4) AMP NUCLEOSIDASE (EC 3.2.2.4) GTP PYROPHOSPHOKINASE (EC 2.7.6.5) GUANOSINE.3,5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE

(EC

3.1.7.2) Pyrimdine and purine metabolism: Nucleic Acid SEQ ID NO 1057 1059 1061 1063 1065 1067 1069 1071 1073 1075 1077 1079 1081 Amino Acid SEQ D0 NO 1058 1060 1062 1064 1066 1068 1070 1072 1074 1076 1078 1080 1082 Identification Code RXN0 1940 F RXA01940 RXA02559 RXA02497 RXN0 1079 F RXA01079 F RXA01084 RXN01 920 F RXAO 1920 RXA0 1080 RXA00867 RXA01416 RXA01 486 NT Stop Function 9333 INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) 581 INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) 6320 INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) 10985 EXOPOLYPHOSPHATASE (EC 3.6.1.11) 35982 RIBONUCLEOSIDE.DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) 4 RIBONUCLEOSIDE.DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1. 17.4. 1) 2062 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1. 17.4. 1) 31842 RIBONUCLEOSIDE-OIPHOSPHATE REDUCTASE 2 BETA CHAIN (EC 1.17.4.11) 908 RIBONUCLEOTIDE REDUCTASE SUBUNIT R2F7 797 NRDI PROTEIN 627 POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) 631 POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) 4 POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) 2006200807 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Contig NT Start NT Stop Function SEC)ID NO SEQID0NO 1083 1084 RXA01678 GR00467 7162 7689 2',3-CYCLIC-NUCLEOTIDE 2-PHOSPHODIESTERASE (EC 3.1.4.16) 1085 1086 RXA01679 GR00467 7729 8964 2',3-CYCLIC-NUCLEOTIOE 2'-PHOSPHODIESTERASE (EC 3.1.4.16) 1087 1088 RXN01488 WV0139 39842 40789 INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) 1089 1090 RXCO0540 CYTOSOLIC PROTEIN INVOLVED IN PURINE METABOLISM 1091 1092 RXCO0560 PROTEIN INVOLVED IN PURINE METABOLISM 1093 1094 RXCO1088 CYTOSOLIC PROTEIN INVOLVED IN PURINE METABOLISM 1095 1096 RXC02624 MEMBRANE SPANNING PROTEIN INVOLVED IN PURINE METABOLISM 1097 1098 RXC02665 PROTEIN INVOLVED IN PURINE METABOLISM 1099 1100 RXC02770 LIPOPROTEIN INVOLVED IN PURINE METABOLISM 1101 1102 RXC02238 PROTEIN INVOLVED IN METABOLISM OF S-ADENOSYLMET-IION INE, PURINES AND PANTOTHENATE 1103 1104 RXC01 946 ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN PURINE

METABOLISM

Pyrimidines: Nucleic Acid Amino Acid Identification Code Conig. NT Sta NT Stop Function SEQ ID NO S EQ IDNO 1105 1106 RXN03171 WV0328 568 1080 URACIL PHOSPHORIBOSYLTRANSFERASE (EC24.2.

9 1107 1108 F RXA02857 GR10003 570 1082 URACIL PHOSPHORIBOSYLTRANSFERASE (E24.2.9) 1109 1110 RXN00450 W0O112 34491 34814 CYTOSINE DEAMVINASE (EC 3.5.4. 1) 1111 1112 F RXA00450 GROW 10 322 5 CYTOSINE DEAMINASE (EC 3.5.4.1) 1113 1114 RXA00465 GRO01 17 337 828 CYTOSINE DEAMINASE (EC354.1) 1115 1116 RXA00717 GROO1B8 3617 4576 RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B (EC 4.2.1.70) 1117 1118 RXA01 894 GRO0542 1622 2476 PHOSPHATIDATE CYTIDYLYLTRANSFERASE (EC 2.7.7.41) 1119 1120 RXA02536 C31300726 8581 7826 BETA-UREIDOPROPIONASE (EC 3.5.1.6) 1121 1122 RXN01209 WV0270 1019 2446 PHOSPHOMETHYLPRIMIDINE KISE (EC27.

4 .7) 1123 1124 F RXA01209 GR00348 1019 2446 PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) 1125 1126 RXN01617 WO0050 22187 22858 PHOSPHOMETHYLPYRIMIDINE KINASE.(EC 2.7.4.7) 1127 1128 F RXA01617 GR00451 2 616 PHOSPHOMETHYLPYRIMIDINE KINASE:(EC 2.7.4.7) 1129 1130 RXCO1600 CYTOSOLIC PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 1131 1132 RXCO1622 CYTOSOLIC PROTEIN INVOLVED IN.PYRIMIDINE METABOLISM 1133 1134 RXC001 28 EXPORTED PROTEIN INVOLVED IN MOTABOLISM OF PYRIDIMES AND ADENOSYIHOMOCYSTEINE 1135 1136 RXCO1709 CYTOSOLIC PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 1137 1138 RXC02207 EXPORTED PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 2006200807 24 Feb 2006 Table 1 (continued) Sugars Trehalose Nucleic Acid SEQ ID NO 1139 1 141 1143 1145 1147 1149 1151 1153 1155 Amino Acid Identification Code Contig.

S-EQ 1-D NO 1140 RXA00347 GROOQI 1142 RXN01239 WV0090 1144 F RX(AO1 239 GR003 1146 RXA02645 GRO07 1146 RXN02355 WV0051 1150 RXN02909 W01 35 1152 RXS00349 1154 RXS03183 1156 RXCO0874 NT Start NT Stop Function 65 246 32921 58 5147 51 714 I 735 38532 1013 30489 .7579 2543 4 39017 TREHALOSE-PHOSPHATASE (EC 3.1.3.12) maltooligosyltrehalose synthase maltooligosyltrehalose synthase maltooligosyltrehalose trehalohydrolase TREHALOSEIMALTOSE BINDING PROTEIN Hypothetical Trehalose-Binding Protein Hypothetical Trehalose, Transport Protein TREHALOSE/MALTOSE BINDING PROTEIN TRANSMEBRANE PROTEIN INVOLVED IN TREHALOSE METABOLISM 2006200807 24 Feb 2006 2 Excluded Genes GenBankM Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A4558 1, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 9519442-AS5 07/20/95 A45585 A45587 AB0O3 132 murC; ftsQ; ftsZ Kobayashi, M. et al. "Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria," Biochem. Biophys. Res. Commun., 236(2):383-388 (1997) ABO 15023 mujrC4 ftsfl A E~fl 1 OClfl 1 4.

tDVIO.JU UtSI ADA I OII I Aj..n. n I flu~nj zojju LS I! Ut MZ AB___20624 D-glutamate rcms AB023377 tkt I transketolase wacna, Mv. et ai. ~A murC gene from Coryneform, bacteria,", App. Mi-jcrobial.

BiotechnoL, 51 (2):223-228 (1999) Kimura, E. et al. "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacierium laclofermentum," Biosci. Biolechnol. Biochem., 60(10): 1565-1570 (1996) Pt5I4~L /u6 gitti; gztD (ilutamine 2-oxoglutarate aminotransferase large and small subunits i 1aconitase AB02T7714 I r~n 1 D 1;I en cat on DrOLem I

I

I

flIY 1 rep; ad Replication protein; aminoglycoside adenyltransferase A t 4 AFrUU)L'f argC dehvdro2enase A Pnn,,rj,; 1 1 A i

I

P; t.

uiuw.rnlne svnrnera~ AF030405 hisF cyclase AF030520 arGArgininosuccinate synthetase A0 1518 Urnithane carhamnlvtr~n'~fpr~p Umithme carbamolvtransferasp i i ArUio,~jZ arou 3-dehvdroaiiin~t~ d4ivd4r~t,,cp 3-dehvdrociuinate deh dra- A CAI Q IZAQ i j I flu ,JJo.rtv rvruvare c~rhnYuI~~ rvruvate -ox e 2006200807 24 Feb 2006 Tabie 2 (conti ued AF038651 dciAE; apt; rel Dipeptide-binding protein; adenine Wehmeier, L. et al. "The role of the Corynebacterium glutamicum rel gene in phosphor ibosy Itransferase; GTP (p)ppGpp metabolism," Microbiology, 144:1853-1862 (1998) pyrophosphokinase AF04 1436 argR Arginine repressor AF045998 impA Inositol monophosphate phosphatase AF048764 argH Argininosuccinate lyase AF049897 argC; argJ; argB; -N-acetylIglutamyl phosphate reductase; argD); argF; argR; ornithine acetyltransferase; NargG; argH acetylglutamnate kinase; acetylorn ithine transminase; ornithine carbam oy ltransfe rase; arginine repressor; argininosuccinate synthase; lyase AF050 109 inhA Enoyl-acyl carrier protein reductase A F050166 Ih isG ATP nhosnhoribosvtransfpracp AF051846 hkA 0 I.

phosphoribosyl-4- im idazolecarboxam ide isomerase A~ i I Ill 'JJ~.U4L Iii Lt H-omoserine U-acetyltransterase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacterium glutamicum," MoL Cells., 8(3):286-294 (1998) AF053071 IaroB Deydoqint synthetase ?~dVUVJ.IO L1-I ulutamnine amidotransterase A VnA'7fA I ft VOV~~J*-t 111Sr, rnospnoribosyi-ATP- *i I Ar 1 i'433 aroA 5-englpyruvyishikimate 3-phosphate synthase

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AflhI~ OA Ar I J uISO' panu L-aspartate-alpha-decarboxylase precursor Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alph a-decarboxylase leads to pantothenate overproduction in Escherichia coli," Appl. Environ. MicrobioL, 65(4)1530- 1539 (1999) AF 124518 1aroD); aroE 3-dehydroquinase; shikimate ehydrogenase ArtiZ'4OUU aroC; aroK; aroti; pepQ horismate synthase; shikimate kinase; 3dehydroquinate synthase; putative cytoplasmic peptidase AF145897 jinhA AF145898 I inhA L 2006200807 24 Feb 2006 2conti AJO0 1436 ectP Transpart of ectoine, glycine -betaine, praline AJO04934 dapD AJU07732 ppc; secG; am!; -ocd; soxA AJO10319 NtY, gn13, gInD; srp; amtP Tetrahydrodipicolinate succinylase (incomplete') Phosphoenolpyruvate-carboxylase; high affinity ammonium uptake protein; putative omithine-cyclodecarboxylase; sarcosine oxidase Involved in cell division; PH1 protein; uridylyltransferase (uridylyl-removing enzmye); signal recognition particle; low affinity ammonium uptake protein C-hioramphenicol aceteyl transferase L-malaite: quinione oxidoredcta-se inou) Peter, H. et al. "Corynebacteriurn glutamicumn is equipped withfuseodr carr iers for compatible solutes: Identification, sequencing, and characterization of the prol ine/ectoine uptake system, Prop, and the ectoine/prol ine/glycine betaine carrier, EctP," J Bacterial., 180(22):6005-6012 (1998) Wehrmann, A. et al. "Different modes of diaminopimelate synthesis and their role in cell wall integrity: A study with Corynebacteriumn glutamicum,"

J

Bacteriol., 180(12):3l159-3165 (1998) Jakoby, M. et al. "-Nitrogen regulation in Corynebacteriumn glutamicuFm; Isolation of genes involved in biochemical characterization of corresponding proteins," FEMS Mlicrabiol., 173(2):303-3 10 (1999) AJ132968 cat AJ224946 1mqo AJ23 8250 ndh ND eyrgns A J_23 8703 poAPorin D5l17429 T rain-sp os abIee ele men tl S3 18 3-1 D84 102 odA 2 -oxoglutarate dehydrogenase E01358 hdh;hk E0130 9 E0 13 75 E01376 trpL; trpE Hornoserine dehydrogenase; homoserine kinase Upstream othe start codon ofhomoserine kinase gene Tryptolphan operon Leader peptide; anthranilate synth-ase Ver et al. slato hia and geti characterization of th 8 ,a rnpsbeelen frm oynbctrma tmicum," Mal. Micrbial, 254(2):395-403 (1994) Uswa Y.ri eta.ofec lnigo h Corynebacteriurn glutariu:Tecanlsfomdcumalo (Brevibacterium lactofermentumn AJ 12036) odhA gene encoding a novel type of 2 -oxoglutarate dehydrogenase," Microbiology, 142:3347-3354 (1996) Katsumata, K. et al. "Production of L-hereoni ne and L-isoleucine,"' Patent: JP 1 9 87232392-A 1 10/12/87 Katsumnata, R. et al. &CP3roduction ofL-thereonine and L-isoleucine," Patent: JP 1 98 7232392-A 2 10/12/87 Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, utilization of tryptophan operon gene expression and production of tryptophan," Patent: JP 1 987 244382-A I 10/24/87 2006200807 24 Feb 2006 2 (continued) E01377 Promoter and operator regions of Matsui, K. et "Tryptophan operon, peptide and protein coded thereby, tryptophan operon utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Hatakeyama, K. et al. "DNA fragment containing gene capable of coding synthetase and its utilization," Patent: JP 1992278088-A 1 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding d iam inopel argon ic- acid aminotransf-erase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A I E04041I Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 E04307 Flavum aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: P 199303097 7-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 52-A 2 03/30/93 108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: RP 1993 184366-A 1 07/27/93 E051 12 Dihydro-dipichorinate synthetase Hatakeyama, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase its use," Patent: JP 1993184371 -A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diamino-pimelic acid dehydroge-nase and its use," Patent: JP,1993284970-A 1 11/02/93 779 1Threnne svxnthas- I 1/ .1 k E061 10 1 Prephen ate dehydratase E061 i1 E06 146 Mutated Prephenate dehydratase Acetohydroxy acid synthetase ohua,,a, et al. UG[lC:VfNA cooing tnreonine synthase and its use," Patent: JP 1993284972-A 1 11/02/93 K ikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993 34488 12/27/93 Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993 34488 1-A 1 12/27/93 lnui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its use," Patent: JP 1993344893-A 1 12/27/93 Sugimoto, M. et al.' "Mtn sakinase gene," patent: JP 1994062866-A 1 03/08/94 Sugimoto, M. et al. "Mutant aspartokinase gene,". patent: JP 1994062866-A 1 03/08/94 E06825 1,.

ap va unIas Mvutated asparto~inase alpha subunit Ii I.

2006200807 24 Feb 2006 2 (continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1 9§4-062866.A 1 E07701 secY Honno, N. et al. "Gene DNA participating in integration of membraneous to membrane," Patent: JP 1994169780-A 1 06/21/94 E08 177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 178, Feedback inh ib ition-relIeased Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 180, E08 18 1, EQ08182 E08232 Acetohydroxy-acid isomeroreductase lnui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," Patent: JP 1994277067-A 1 10/04/94 E08234 secE Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," JP 1994277073-A 1 10/04/94 E08643 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in synthetase promoter region coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08649 Aspartase Kohama, K. et al "DNqA fragment having promoter function in coryneformn bacterium," Patent: JP 199503 1478-A 1 02/03/95 E08900 Dihydrodipicolinate reductase Madori, M. et al. "DNA fragment containing gene coding Dihydrodipicolinate; reductase and utilization thereof," Patent: JP 1995075578-A 1 03/20/95 E08901 fliaminnrimeic .A A-l 1 A I Serine hydroxymetrlyltransferase P I114At I179 E12759, transposase E 12764 Arginyl-tRNA synthetase; diamino-pimelic acid decarboxylase IviadUU[, et. iat. UNA iragment containing gene coding Diaminop-imelic acid decarboxylase and utilization thereof," Patent: JP 1995075579-A 1 03/20/95 Hatakeyama, K. et al. "Prioduction of L-trypophan," Patent: JP 1997028391-A 1 02/04/97 Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18?97 Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18/97 Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18/97 Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18/97 Moriya, M. et al. "Am-plification of gene using artificial transposon," Patent:, JP 1997070291 -A 03/18/97 11~7L'7 I ~1 u inyuroaipicolinic acid synttietase Ci I /lIIV aspartokinase r i i I iLl Uihydrodipicolinic acid reductase 2006200807 24 Feb 2006 Table 2 (continued) E13655 Glucose-6-phosphate dehydrogenase Hatakeyama, K. et al. "Glucose-6-phosphate dlehydrogenase and DNA capable coding the same," Patent: JP 199722466 1I-A 1 09/02/97 LO 1508 1IlvA Threonine dehydratase Moeckel, B. et al. "Functional and structural analysis of the threonine dehydralase of Corynebacterium glutamicum," J Bacteriol, 174:8065-8072 (1992) L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3 -deoxy- D-arab inoheptulIoson ate-7-ph osph ate synthase gene," FEMS Microb jot. Lemt, 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit; molecular analysis of the ilvB-ilvN-ilvC operon," J Bacteriol., 175(17):5595- Acetohydroxy acid isomeroreductase 5603 (1993) L18874 PtsM Phosphoenolpyruvate sugar Fouet, A et al. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransferase system: expression in Escherichia coli and homology to enzymes 11 from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, I.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme 11 and analyses of the deduced protein FEMS Microb jot. Let., 119(1-2): 13 7-145 (1994) L27 123 aceB Malate synthase Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J Microbial Biatechnol., (1994) L27 126 Pyruvate kinase Jetten, M. S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacteriuni glutamicum," App!. Environ. Mlicrobial., 60(7):2501-2507 (1994) L28760 aceA Isocitrate lyase L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et al. "Mqleqtllar cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium J Bacterial., 1 77(2):465-467 (1995) M13774 Prephenate dehydratase Follettie, M.T. et al. "Mo ecular cloning and nucleotidle sequence of the Corynebacterium glutamnicumn pheA gene," J BacteriaL, 167:695-702 (1986) *M16175 5S rRNA Park, Y-H. et al. "Phylogenetic analysis of the coryneformn bacteria by 56 sequences," J Bacteria!., 169:1801-1806 (1987) M 16663 trpE Anthranilate synthase, 5' end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermnentum, a glutam ic-acid-producing bacterium," Gene, 52:191-200 (1987) M 16664 trpA Tryptophan synthase, 3 end Sano, K. et al. "Structure and function of the trp, operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium," Gene, 1-200 (1987) 2006200807 24 Feb 2006 Table 2 (continued) M25819 Phosphoenolpyruvate carboxylase O'Regan, M. et al. "Cloning and nucleotide sequence of the Phosphoenolpyruvate carboxy lase- coding gene of Corynebacterium ATCC13O32," Gene, 77(2):237-251 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a hi gh DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J Gen.

Microbial, 138:1167-1175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J. Gen.

138:1167-1175 (1992) M89931 aecfl7 hrn& vhhw I ti r.'s i n. i- fl4 1, 1n uptake carrier; hypothetical protein yhbw Kossot, 1. et al. In he orynebacterium glutamnicumn aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine," J Racteriol., 174(9):2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the bmQ gene product," Arch. Microbial, 1 69(4):303 -312 (1998) S59299 1 tM i T A i e. gwe kproMOLei) Herry, D.M. et al. "Clor hyperproducing strain oi mutation in the trp leade (1993) O'Gara, J.P. and Dunica Corynebacterium glutan T I I I ICA 4Z i i

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'.41 I 1rpLJ iintniraniiate pflospfloribosyltransterase De awrtmn,,t Uiv in,,t.

r' V, U 13922 cgllM; cgllR; clIIR Putative type 11 5-cytosoine Schafer, A.et al. "Cloni methyltransferase; putative type II stress-sensitive restrictic restriction endonuclease; putative type I or .13032 and analysis of it type III restriction endonuclease coli," J Bacterial, 176( Corynebacterium glutan Escherichia co U 14965 recA U31224 ppx Ankri, S. et al. "Mutatio biosynthetic pathway: A 178(15):4412-4419 (195 U3 1225 proC L-proline: NADP+ 5-oxidore-ductase Ankri, S. et al. "Mutatio biosynthetic, pathway: A 178(15):4412-4419 (199 ting oth rgene cluster from a tryptophanf Corynebacterium glutamicum: identification of a ~r sequence," AppI. Environ. Microbial, 59(3):791-799 in, L.K. (1994) Complete nucleotide sequence of the licum ATCC 21850 tpD gene." Thesis, Microbiology College Galway, Ireland.

ng nd harcterization of a DNA region encoding a n system from Corynebacterium glutam icum ATCC srole in intergeneric conjugation with Escherichia 23),7309-7319 (1994); Schafer, A. et al. "The icum cglIM gene encoding a 5-cytosine in an McrBCli.strain," Gene, 203(2):95- 101 (1997) ng in the Corynebacterium glutamicumnproline natural bypass of the proA step," J Bacterial, ~6) ns in the Corynebacterium glutamnicumnproline natural bypass of the proA step," J Bacterial., P6) ns in the Corynebacterium glutarnicumpro-line natural bypass of the proA step," J Bacterial., 6) I LJV ug proo un~un !;gamma glutamyl kinase;similar to Disomer specific 2-hydroxyacid dehydrogenases Ankri, S. et al. "Mutatio biosynthetic pathway: A 178(15):4412-4419 (199 I I 2006200807 24 Feb 2006 Table 2 (contin _ed) U3 1281 bioB Biotin synthase Serebriiskii, "Two new members of the bio B superfamily: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatumn and Corynebacteriumn glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC Thiosulfate sulfurtransferase; acyl CoA Jager, W. et al. "A Corynebacterium glutamicumn gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins," Arch. Microbil. 166(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamnicumn gene conferring multidrug resistance in the heterologous host Escherichia coli," J Bacterial., 1 79(7):2449-245 1 (1997) U43536 clpB Heat shock ATP-binding protein U53587 aphA-3 3'5' -aminoglycoside phosphotransferase U 89648 Corynebacterium glutamicum unidentified sequence involved in histidine biosynthesis, sequence X04960 trpA; trpB; trpC; trpD; Tryptophan operon Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium lactofermentumn tryptophan operon," Nucleic Acids Res., 13-10114 (1986) X07563 lys A DAP decarboxylase (meso-diaminopimelate Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium decarboxylase, EC 4.1.1.20) glutamicumn and possible mechanisms for modulation of its expression," Mal.

Genet., 212(l):112-119 (1988) X 14234 EC 4. 1.1.31 Phosnhoenolnvnivate carhnvulas~e Pit D 1 11 ~m.

rJ 111 1 CA_ 0 .1 rructose-oispnosphate aidolase 4.

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A.JJ7 7J uiip/ L-2, 3-dihydrodipicolinate synthetase (13C 4.2. 1.52) manna~*U, .J et al. I he rziuspnenoipyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression," Mal. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolutio," Plant. Mal. Biol., 21 (3):487-502 (1993) Von der Osten, C.H. et aPl."Molecular cloning, nucleotide sequence and finestructural analysis of the Corynebacterium glutamicumn fda gene: structural comparison of C. glutamnicumn fructose-I, 6-biphosphate aldolase to class Iand class 11 aldolases," Mat. M~icrobial., Bonnassie, S. et al. "Nucleic sequence of the dapA gene from Corynebacteriumn glutamicum," Nucleic Acids Res., 18(21 ):642 1 (1990) Cianciotto, N. et al. "DNA sequence homology between at" B-related sites of Corynebacteriumn diphtheriae, Corynebacterium ulcerans, Corynebacteriumn glutamicumn and the attP site of lambdacorynephage," FEMS. Microbial, Lett., 66:299-302 (1990) Marcel, T. et al. "Nucleotide sequence and organization of the upstream region of the Corynebacteriumn glutamnicumn lysA gene," Mol. Microbial., 4(1 1):1819- X54223 i i AttQ_-,.olt-, i a i i

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arg &ystA Arginyl-tRNA synthetase; Diaminopimnelate decarboxylase

I

1830 (1990) 2006200807 24 Feb 2006 ab le 2 (co n tin uedl X55994 trpL; trpE Putative leader peptide; anthranilate Heery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamnicumn component I trpE gene," Nucleic Acids Res. 18(23):7138 (1990) X56037 thrC Threonine synthase Han, K.S. et al. "The molecular structure of the Corynebacterium glutamicumthreonine synthase gene," Ma t. MicrobioL, 4(10):1693-1702 (1990) X56075 attB-related site Attachment site Cianciotto, N. et al. "DNA sequence homology be-tween att B-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacteriumn glutamicum and the attP site of lambdacorynephage," FEMS. Microbiol, Left., 66:299-302 (1990) Lvs%_-an1ia* lysu-betao Asna lanase-al ha Q11Lnn:t asd Aspartokinase-beta subunit; aspartate beta semialdehyde dehydrogenase A -141411 a a 1, t i 0 r r1b f, Glyceraidehyde-3-phosphate; phosphoglycerate kinase; triosephosphate isornerase V A 1 -AL i -17 U4 Glutamate dehydrogenase X603 12 1 1-1 i I ys ne permease I. I. Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase from Corynebacterium glutamicum," MoL Microbiol., 5(5):1197-1204 (1991); Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta-semialdehyde dehydrogenase gene asd in Corynebacterium glutamicum," MoL Gen. Genet., 224(3):317-324 (1990) Eikmanns, B.J. "Identi Fication, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3 -phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J Bacteriol., 174(19):6076-6086 (1992) Bormann, E.R. et al. "Molecular analysis of the Coryn ebacteriu i _glutami cum gdh gene encoding glutamate dehydrogenase," A&L A4icrobiol., 6(3):317-326 (1992) Seep-Feldhaus, A.M. et al. "Molecular analysis of the Corynebacterium glutarnicurn lysl gene involved in lysine uptake," MoL MicrobioLl 5(12):2995- 3005(1991) Joliff, G. et al. "Cloning d nucleotide sequence of the csFI gene -encoding PSI, one of the two maj&r. secreted proteins of Corynebacterium glutamicum: The deduced N-terminal region of PSI is similar to the Mycobacterium antigen 85 complex," Mol. MicfobioL, 6(16):2349-2362 (1992) Eikmanns, B.J. F al. _"Clo ing sequence, expression and transcriptional analysis of the Corynebacterium glutarnicum gItA gene encoding citrate synthase," UicrobioL, 140:1817-1828 (1994) Peyret, J.L. et al. "Characterization of the cspB gene en surface-layer protein in Corynebacterium glutamicum," Mol. Microbiol., 9(1):97-10) (1993) Bonamy, C. et al. "Identification of IS 120b, a Corynebacterium glutamicum IS3-related insertion sequence and phylogenetic analysis," A161. Microbiol., 14(3):571-581 (1994) ACOU/8 Psi pr VAA 1 1 1 i Citrate synthase X67737 I dapB Dihydrodipicolinate reductase V n I U 1UJ Surface layer pr i i Ao o 1 U4 !S3 related insertion element 2006200807 24 Feb 2006 Table 2_(continued) X70959 leuA Isopropylmalate synthase Patek, M. et al. "Leucine synthesis in Corynebacterium glutamniculm: enzymne activities, structure of leuA, and effect of leuA inactivation on lysine Appi. Environ. Microbiol., 60(1): 133-140 (1994) X71489 icd Isocitrate dehydrogenase (NADP+) Eikmanns, B.J. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacterium glutamicum lcd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J. BaclerioL, X72855 GDHA Glutamate dehydrogenase (NADP+) 177(3):774-782 V7Ycnlj.~ I I rntrA 1 5-meffivi to I-an mistan- X70584 r X75085 1 A Vr, OA 1 A j t rama1IsjdIitrate lyase; t X76875 t A -TD- t, aU U11 L v77nVAi.. 4 Eliongaion factor I u -l lJOt rc.

I-eery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of Corynebacterium glutamicum encoding resistance to Biochem. Biophys. Res. Conmun., 201(3): 1255-1262 (1994) Fi'tzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutam icum and Brevibacterium lactofermnentum," Appi.

Mlicrobiol. Biolechnol, 42(4):575-580 (1994) Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme," J BacterioL, 176(12): 3474-3483 (1994) Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes," Aneonie Van Leeurwenhoek 64:285-305 (1993) Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes," Anlonie Van Leeuwenhoek, 64:285-305 (1993) Billman-Jacobe, H."N-ucleotide sequence of a recA gene from Corynebacterium glutanicjm," DNA Seq., 4(6):403-404 (1994) Reinscheid, D.J. et al. "MIalate synthase from Corynebacterium glutamicumpta-ack operon encoding phosphotransacetylase: sequence analysis," Microbiology, 140:3099-1108 (1994) Rany, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia from within the radiation of Rhodococcus species," Microbiol., 141:523-528 (1995) Kronemeyer, W. et at. "Structure of the gIuABCD cluster encodingth glutamate uptake system of Corynebacterium glutamicum," J Bacterial, 177(5):1 152-1158 (1995) Wehrann A.et at. "Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of Escherichia coti Microbiology, 40:3349-56 (1994) 1 I A iaq91 acetB Malate synthase A OVU/Y~ 165 rDNIA 16Sb ribosomal RNA X81191 1 1 A 1 0 1 gluD 'JJuti1IIadL uptake system VQl~f 1 iJ.. IX U I .J 17 uap E Succinyidiam inopimelt eucnls 2006200807 24 Feb 2006 2 (continued) rnb trund d cdfo X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et al. "Phylogeny of the genus Corneacteimddcdfo analyses of small-subunit ribosomal DNA sequences," Int. J. Syst Bacteriol., (1995) X82928 asd; IysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J 177(24):7255-7260 (1995) X82929 proA Gamma-glutamyl phosphate reductase Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J 1 77(24):7255-7260 (1995) X84257 16S rDNA 16S ribosomal RNA Pascual, C. et "Phylogenetic analysis of the genus Corynebacterium based 16S rRNA gene sequences," Int. J Syst. Bacteril., 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et "Functional analysis of sequences adjacent to dapE of C.

glutamicum praline reveals the presence of aroP, which encodes the aromatic acid transporter," J Bacterial., 1 77(20):599 1-5993 (1995) X86 157 argB; argC; argD; Acetylglutamate kinase; N-acetyl-gamma- Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine argF; argJ glutamyl[-phosphate reductase; biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early acetylornithine aminotransferase; omnithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbam oylItrans ferase; glutamate Nosp a e nety trans rase; acetate runase YSQR21 #Q AttachmIffent site VOA7~ t I~ .J U Promoter fragment F' I X90357 T I LIIIULer gmem 1-2IIL Reinscheid, D.J. et "Coig euneaayi, xrsinadiatvto of the Corynebacterium glutamnicumn pta-ack operon encoding phosphotransacetylase and acetate kinase," Microbiology, 145:503-513 (1999) Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J Bacterial., 178(7):1996-2004 (1996) Patek, M. et al, "Promoters from Corynebacteriumn glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et "Promoters from Corynebacteriumn glutam icum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacteriumn glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) ~,nnrn A)Pk3JO P'romoter fragment FI10 YQA~Q T I I X9035Q romOLer Itagment r H A I 2006200807 24 Feb 2006 X90360 X90361 X90362 X90363 X90364 X90365 X90366 X90367 X90368 X93513 X93514 X95649 X96471 I .1 Promoter fragment F22 amt betP orf4 lysE; IysG Promoter fragment F34 Promoter fragment F37 Promoter fragment -F45 Promoter fragmentF Promoter fragment -F75 Promoter fragment PFI1 Promoter fragment PF104 Promoter fragment PFiO9 Ammonium transport systen Glycine betaine transport sy! Lysine exporter protein; Lys regulator protein Table 2 continued Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309(1996) Patek, M. etal. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. IPromoters from C. glutamicum: cloning, molecuar a and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoers from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutaicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and pearph for a consensus motif," Microbiology, 142:1297-1309 (1996) I' Siewe, R.M. et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier.of Corynebacterium glutamicum," J. Biol. Chem., 271(10):5398-5403 (1996) stem Peter, H. et al. "Isolation, characterization, and expression of the Corynebacteriurn glutarnicurn betP gene, encoding the transport system for the compatible solute glycine betaine," J. Bacteriol., 178(17):5229-5234 (1 996) Patek, M 11' 91 11 1111! (11, 11, 1 11 X 11 1! (1111 1 11! dap 13- YP -,F2 dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis," Biolechnol. Left, 19:1113-1117 (1997) mne export Vrljic, M. et al. "A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum," Mol.

Microbiol., 22(5):815-826 (1996) 2006200807 24 Feb 2006 Table 2 otiud) X96580 jpan B; pa 3xyIB oxo)taot Sahm, H. et al. )D-pantothenate sythsi in. veace i i m X96962 X99289 alanine ligase; xyiulokinase insertion sequence IS 1207 andtrnpss Elongation factor P use of panBC and genes encoding L-valine synthesis for D-pantothenate overproduction," App!. Environ. M'icrobil., 65(5): 1973-1979 (1999) YOO 140 lhrb I-lomosrine kinase Y00 151 1 ddan Meso -di aminop1Imat Ddehydrogenase (EC 1.4.1.16) Y00476 I thrA I H-omoserine dlehydrogenase Y00546 Ihorn; t h r _B Y08964 murC, ftsQ/divD; ftsZ omoserine dehydro kinase U PD -N -acetylii,,ii :11 :lil a I I: I nine Ii ie, division initiation protein or cell division protein; cell division protein _if- riHligha ini pro ine tvan'sp-ortsyste m Pyru atecar Dxylase Hob, A. t al. Cntificin characer-iationoftagnd o g eogiation fco i the ami gne-ci fro ue Brevibacteriun lactofermentum .Gn Geoneba iu 259 l):97-104 r (1998))" ee 1821-2 (97 Pete, H. e ll oain sfteugence of heCory 7nebatei e thBgn comftBib acoltes"rc icfrobiol.," Nucle:3i I AisR .1(99 :92( 87 Peterendsch g ta.Pyvaearoysefom Corynebacterium ltmc"NcecAisRes.

M1crob:olog (1597 (98 Pate, M. et al. "Nlothde Seuene ofrt-omoernebatr g5utam:cum98 (p1M9rbo87i)eho.,5():24 (98 Meoeau, SU. et al. "ite-seii iesuntera n firnepsh l-ag Ph i- The PtrHta."Corynebacterium glutamicum is-tr oequippe ith fcour. secondary2 cari I for cmaibl soluts:Identifica io, seunigad characterizatinadcho s o n of te cprolinedciatrztin'falo-afnt uptake system, frorn heetiepoln/lcn bemtie rrie," ctP,. Bacriol., 18(2):143051 (1997) Y09 16 3 pu-tp 7Y09548 'pyc Y09578 (1]1uB 3 -sppIIale dehydrogenase Y 1247T Attachment site bactriophage- Pi.1 Y 12537 -proP? Proline/ectoine uptake system pr-otein 2006200807 24 Feb 2006 2 (cant Y13221 g InIA Glutamine synthetase I Yl16642 l _pd Dydlpomddedrgase Y 18059 Attachment site Corynephage 304L Z21501 argS; lysA Z21502 dapA; dap-B_ -7 ArginlRN syntCetas J dIam inopi-melate decarboxylase (partial) FiyroiIc !:Ijnate synt1hase; dihydradipicolinate reductase inued) Jakoby, M. et al. "isolto of.11 CTtorynebacterium glutamiu ~Agn rencoding glutammne synthetase FEMS Microbial. Left., 154(1):81-88 (1997) Moreau, S. et al. "Analys is of the! inte Ugi 11rai funion (1s of phi;304L: An integrase module among corynephages," Virology, 255(1): 150-159 (1999) Oguiza, J.A eta."_eeecdn rIltAsnhesei located in the upstream region of the lysA gene in Brevibacterium lactofe rmentum: Regulation of argS-lysA cluster expression by arginine,"

J.

Bacterial., 175(22):7356-.7362 (1993) Pisabarro, A. et al. cluster of three gene ::pA orf2 and 1.pB ofq 11 Brevibacterium lactofermentum encodes dlihydrodipicolinate reductase, and a third polypeptidle of unknown function," J. Bacterial., 1 75(9):2743-2749 (1993) Malumbres, M.!et a. "AaIs and.5 expressio of1) theS thr geef the encoded threonine synthase," Appi. Environ. Microbiol., 60(7)2209-2219 (1994) Z29563 Ihr Teoiesnhase Z46753- 16S -rDNA Gene fo-6ibsmlRA -48T SigA sigma factor Oguiza, J.A. et al "Multiple sigmia facto genes in Brevibacteriumn lactofermentum: Characterization of sigA and sigB," J. Bacterial., 178(2):550.

553 (1996) Z49823- gialE; dtxR Cataly'tc activity UDP-galactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4 -epimerase of epimerase; diphtheria toxin regulatory Brevibacterium lactofermentrm is coupled transcriptionally to the dmdR Z49824 rfi; sgprotinBsgafco gene," Gene, 177:103-107 (1996) Z 4 9 ff 4 r f I i g S i g BO g U i z a .A e t a l M u l t i p l e s i g m a f a c t o r g -e n e s i -n B r e v i b a c -t e r -i u m the ulsedersi It F i s blidtatebihe deso relie on~ an, incrre star con adned Dth repreentos ofl ah frgment ofpthcatua i coing rinlon e ta -91- TABLE 3: Corynebacteriumn and Brevibacterium Strains Which May be Used in the Practice of the Invention ,ATQ. I-ERNVM,-NRRT~fw~ t. ,twrvo I Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 19351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes 19353 Brevibacterium ammoniagenes 19354- Brevibacterium ammoniagenes 19355 Brevibacterium ammoniagenes 19356 Brevibacterium ammoniagenes 21055 Brevibacterium jammoniagenes 21077 Brevibacterium lammoniagenes 21553 Breibaterum ammoniagenes 21580 Brevibacterium lammoniagenes 39101 Brevibacterium lbutanicum 21196 Brevibacterium Idivaricatum 21792 P928I Brevibacterium Iflavum 21474 Brevibacterium Iflavum 21129 Brev bacterium Iflavum 21518 Brevibacterium flavum BI 1474 Brevibacterium flavum B1 1472 Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium fiavum 21517 Brevibacterium flavum 21528 Brevibacterium flavum 21529 Brevibacterium flavum B1 1477 Brevibacterium fiavum BI 1478 Brevibacterium flavum 21127 Brevibacterium favum B] 1474 Brevibacterium healii 15527 Brevibacterium ketoglutamicum 21004 iBrevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacteriuni lactofermentum Brevbactrium lactfermntu Brevibacterium lactofermnentum 77 flrpvihactpri,, I rf '-no Brevibacterium FBrevibacterium Brevibacterium Brevibacterium ii ILlS IUI II LI /70 1t 4P -t i- j I actoI1~ jumej ull ZI I/YY 1-f- C. 11O. I I I -t fi m ILI OUU I I C_ r I lactoferment tumI ZI1su r t BreviTb-acterium ['lactofermentum BI 11471

I-

I-

I.

Brevibacterium lactofermentum 21086 Brevibacterium lactofermentu 21420 Brevibacterium lactofermientum 21086 Brevibacterium lactofermentum 31269 Brevibacterium linens 97 Brevibacterium Ilinens19 1 00Brevibacterium Ilinens 8377 Brevibacterium lparaffinolyiu 11160 CN2I Brevibacterium Ispec. 777 Breiatru spcB___ 717.73 revibacterium -spec.140 Brevibacteriu-m Ispec. 16047.3 If~revjbacterjum Ispec. 21864 Brevibacterium Ispec. 21865 Brevibacterium Ispec. 21865 Brevibacterium Ispec.194 Crebacterium apetocidpu 1246 lCorynebacterium lacetoacidophilum 213870 Corynebacterium lacetagaichium 138147 Corynebacterium jacetoglutamicum B 11473 Corynebacterium lacetoglutamicum 158067 Corynebacterium lacetoglu tamicum 214906 Corynebacterium acetoglutarnicum 31270 Corynebacterium lacetophilum -B3671 Corynebacterium jammoniagenes 6872 2399 Corynebacteriu amoniagenes 15511 Corynebacteriu fujokense 21496 Corynebacterium jglutamicum 14067 Corynebacterium i gutamicum 39137 lCoryn ebacterium Iglutamicum 21254 ICorynebacterium :glutamicum 21255lCorynebacterium Iglutarnicum 31830 Corynebacterium glutamicum 13032 ICorynebacterium Iglutarnicum 1430 Corynebacterium Iglutamicum 15455 Corynebacteriu n glutamicum 13058 Corynebacterium glutaniicum 13059 Corynebacterium glutamicum 13060 Corynebacterium glutamicum 21492- Corynebacterium glutamicum 21513 Corynebacterium glutamicum 21526 Corynebacterium glutainicum 21543 Corynebacteriu-m glt m 13287 Corynebacterium glutamicum 21851 'Corynebacterium glutaniicum 21253 Corynebacterium glutamicum 21514 Corynebacterium glutamicum 215 16 Corynebacterium glutamicumiil~l~l 2129 93 EMUS--,: d-)IACC FR N)t EIN&IB CS~NT~ SMZ- Corynebacterium glutamicum 21300 Corynebacterium glutamicum 39684 Corynebacterium glutamicum 21488 Corynebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutamicum 21 158 Corynebacterium glutamicum 21159 Corynebacterium glutamicum 21355 Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutamicum 21565 Corynebacterium glutamicum 21566 Corynebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacterium glutamicum 21572 Corynebacterium glutamicum 21573 Corynebacterium glutamicum 21579 Corynebacterium glutamicum 19049 Corynebacterium glutamicum 19050 Corynebacteriuni glutamicum 19051 Corynebacterium glutamicum 19052 Corynebacterium glutamicum 19053 Corynebacterium glutamicum 19054 Corynebacterium glutamicum 19055 Coryriebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium glutamicum 19058 Corynebacterium glutamicum 19059 Corynebacterim glutamicum 19060 Coryne bacterium glutamicum 19185 Corynebacterium glutamicum 13286 Corynebacterium glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium glutamicum 21544 Corynebacterium glutamicum 21492 Corynebacterium glutamicum B8183 Corynebacterium glutamicum B81 82 Corynebacterium glutamicurn B12416, Corynebacterium glutamicum IB 124171 94 spies Ryi RL TJCM CB 7 N C DSMV Corynebacterium glutamicum B___1124181_ Corynebacterium glutamicum B! 11476 Corynebacterium glutamicum 21608 Corynebacterium Ililium P973 Corynebacterium nitrilophilus 21419 ____11594 Corynebacterium spec. ______P4445 Corynebacterium spec. ______P4446 Corynebacterium spec. 31088 Corynebacteriumn spec. 31089 Coryn ebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 20145 Corynebacterium spec. 21857 Coryne bacterium spec. 21862 Corynebacterium Ispec. 21863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA CECT: Coleccion Espanola de Cultivos Tipo, Valencia. Spaini NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Centraalbureau voor Schimmelcultures, Baamn, NL NCTC: National Collection of Type Cultures, London, UK DSMZ: Deutsche Saxnmlung von Mikroorganismen und Zelikulturen, Braunschweig, Germany For reference see Sugawara, H. et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fungi and yeasts edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

2006200807 24 Feb 2006 ID length Genbank Hit

(NT)

Table 4: Alig3nment Results Length Accession Name of Genbank Hit rxaOOO23 3579 rxaOOO44 1059 rxaOOO64 1401 GB-EST33:A1776129 483 GB-EST33:A1776129 483 EMPAT:E11760 6911 A1776129 EST257217 tomato resistant, Cornell Lycopersicon esculentumn cDNA Clone CLER17D3, mRNA sequence.

A1776129 EST257217 tomato resistant, Cornell Lycopersicon esculentumn cDNA clone cLER1 7D3, mRNA sequence.

E11760 Base sequence of sucrase gene.

GB-PAT: 126124 GB_BA2:ECOUW89 GBI_PAT:E16763 GB-HTG2:AC007892 Source of Genbank Hit Lycopersicon esculentum Lycopersicon esculentum Corynebacterium glutamicum Unknown.

Escherichia call Corynebacterium glutamicumn Drosophila melanogaster Drosophila melanogaster 40.956 29-Jun-99 6911 176195 2517 134257 homolocly Date of (GAP) Deposit 126124 U00006 E16763 AC007892 Sequence 4 from patent US 5556776.

E. coli chromosomal region from 89.2 to 92.8 minutes.

gDNA encoding aspartate transferase (AAT).

Drosophila melanogaster chromosome 3 clone BACR02003 (0797) RPCI-98 02.0.3 map 998-99B strain y; cn bow sp, SEQUENCING IN PROGRESS **,113 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO2003 (0797) RPCI-98 02.0.3 map 998-998 strain y: cn bw sp, SEQUENCING

IN

PROGRESS**, 113 unordered pieces.

42,979 08-OCT.

1997 (Rel.

52, Created) 42,979 07-OCT- 1996 39,097 17-DEC- 1993 95.429 28-Jul-99 31,111 2-Aug.99 GBIITG2:AC007892 .134257 AC007892 31,111 2-Aug-99 rxa00072 rxaO01l05 798 rxaOOlO06 579 1170 GBBA1:MTVOO2 56414 GB -BA1:ECU29581 71128 GB-BA2:AE000366 10405 GB-EST15:AA494237 367 GB_BA2:AF161327 2021 GB-PAT:ARO4 1189 654 GBPR4:AC0071 10 148336 AL008967 U29581 AE000366 AA494237 AF161327 AR04 1189 AC0071 10 Mycobacterium tuberculosis H37Rv complete genome; segmet 12/62 Escherichia coli K-12 genome; approximately 63 to 64 minutes.~ Escherichia col K-12 MG1655 section 256 of 400 of the complete genome.

ng83f04.sl NCICGAP Pr6 Homo sapiens cDNA clone IMAGE:941407 similar to SW:DYR_-LACCA P00381 DIHYDROFOLATE

REDUCTASE;,

mRNA sequence.

Corynebacterium diphtheriae histidline kinase ChrS (chrS) and response regulator ChrA (chrA) genes, complete eds.

Sequence 4 from patent US 5811286.

Homo sapiens chromosome 17, clone hRPK.472 -J -18, complete sequence.

Homo sapiens chromosome 19 clone CIT-HIPC_490E211--

SEQUENCING

IN PROGRESS 93 unordered pieces.

Homo sapiens chromosome 19 clone CIT-HSPC 490E21, ~SEQUENCING IN PROGRESS 93 unordered pieces.

Mycobacteriurn tuberculosis Escherichia col Escherichia coli Homo sapiens Corynebacterium diphtheriae Unknown.

Homo sapiens Homo sapiens Homo sapiens 37,753 35,669 35,669 42,896 40,210 41,176 36,783 40.296 40,296 17-Jun-98 14-Jan-97 12-Nov-98 20-Aug-97 9-Sep-99 29-Sep-99 1999 2-Sep-99 2-Sep-99 GB-HTG3,AC008537 170030 AC008537 GB-HTG3:AC008537 170030 AC008537 2006200807 24 Feb 2006 rxaO0l 16 1284 GB-BA2:AF062345 16458 AF062345 G BPAT:11 B647 GBGSS13:AQ44619 7 rxaOOl3l 732 GBBA1:MTY2OBII1 GB BA1 :SAR7932 GB-BA1 :MTY2OBl 1 rxaOO132 1557 GBBA1:MTY2OB1 GB_1N2:TVU40872 GBHTG6:AC010706 1059 rxa00146 1464 rxaO0l47 1302 GBBA1:MTCY2B12 GB SAl :PSEPYRBX GB-BA1 :LLPYRBDNA GBBA1:MTCY2B12 GBBA1:.MTCY154 GB-BA1 :MSGY1 54 GBBA1:MTCY2B12 GBBA1:MSGB937C GBBA1:PAU81259 3300 751 36330 15176 36330 36330 1882 169265 20431 2273 1468 20431 13935 40221 20431 38914 7285 118647 AQ446197 Z951 21 AJ007932 Z95121 Z95121 U40872 AC01 0706 Z81011 L19649 X84262 Z81 011 Z98209 AD000002 Z8101 1 L78820 U81259 Table 4 (continued) Caulobacter crescentus Sstl (sstl). S-layer protein subunit (rsaA), ABC transporter membrane forming unit (rsaE), putative GDP-mannose-4,6dehydratase (IpsA), putative acetyltransferase (lpsB), putative perosamine synthetase (IpsO), putative mannosyltransferase (IpsO), putative ma nnosyltra nsfe rase (IpsE). outer membrane protein (rsaF), and putative perosamine transferase (IpsE) genes, complete cds.

Sequence 6 from patent US 5500353.

nbxbOO62Dl6r CUGI Rice BAC Library Oryza sativa genomic clone nbxbOO62D16r, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 139/162.

Streptomyces argillaceus mithramycin biosynthetic genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 139/162.

Mycobacterium tuberculosis H37Rv complete genome: segment 139/162.

Trichomonas vaginalis S-adenosyl-L-homocysteine hydrolase gene, complete cds.

Drosophila melanogaster chromosome X clone BACR36D15 (D887) RPCI-98 36.D.1 5 map 13C-1 3E strain y; cn bw sp. SEQUENCING IN PROGRESS unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 61/1 62.

Pseudomonas aeruginosa aspartate transcarbamnoylase (pyrB) and dihydroorotase-ike (pyrX) genes, complete cds's.

L.leichmannii pyrB gene.

Mycobacterium tuberculosis H37Rv complete genome; segment 61/1 62.

Mycobacterium tuberculosis H37Rv complete genome; segment 121/1 62.

Mycobacterium tuberculosis sequence from dlone yl 54.

Mycobacterium tuberculosis H37Rv complete genome; segment 61/1162.

Mycobacterium leprae cosmid B937 DNA sequence.

Pseudomonas aeruginosa dihydrodipicolinate reductase (dapB) gene, partial cds, carbamnoylphosphate syrrlhetase small subunit (carA) and carbamoylphosphate synthetase large subunit (carB) genes, complete cds, and FtsJ homolog (Wts) gene, partial cds.

rxaO~~~~~~~~~~lSGetmye 1233clo GBB1:S9BI 33291510 Srpo cscelclr 5266 10Fb9 Caulobacter crescentus Unknown.

Oryza sativa Mycobacterium tuberculosis Streptomyces argillaceus Mycobacterium tuberculosis Mycobacterium tuberculosis Trichomonas vaginalis Drosophila melanogaster Mycobacterium tuberculosis Pseudomonas aeruginosa Lactobacillus Ieichmannii Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Pseudomonas aeruginosa 36,235 36,821 38.124 43,571 41,116 39.726 36,788 61,.914 51,325 63,365 56,080 47,514 60.714 39,229 36,618 61,527 59,538 55,396 19-007- 1999 07-OCT- 1996 8-Apr-99 17-Jun-98 15-Jun-99 17-Jun-98 17-Jun-98 31 -OCT- 1996 22-Nov-99 18-Jun-98 26-Jul-93 29-Apr-97 18-Jun-98 17-Jun-98 03-DEC- 1996 18-Jun-98 1 5-Jun-96 23-DEC- 1996 rxaOO156 1233 GB-BA1:SC9BIO 33320 AL009204 Streptomyces coelicolor 52,666 10-Feb-99 2006200807 24 Feb 2006 GBBA2:AF002133 15437 AF002133 rxaOOl66 783 rxa00198 672 rxaOO216 1113 rxaOO219 1065 rxa00223 1212 rxa00229 803 GBBAI:D85417 GBHTG3:AC008167 GB-HTG3:AC0081 67 GB-HTG4:ACO1O1 18 GBBAl :AB024708 GB-BA1 :AB024708 GBEST24:A1232702 GBIITG2:I-SDJ85OE GBHTG2:HSDJ85OE 9 GBPR2:CNS01DSA GB_-ITG2:AC005079 -0 GB-HTG2ACO00S79 GBHTG2-AC005079 -1 GBBA1:PPEA3NIF GB.BA2:AFI 28444 GB-HTG4:AC0101 11 GBBA2:AF124518 GBPR3:AC004593 GBIITG2:AC006907 7984 174223 174223 80605 8734 8734 528 117353 117353 159400 110000 110000 110000 19771 2477 138938 1758 150221 188972 D85417 AC0081 67 AC008167 AC0 10118 AB024708 AB024708 A1232702 AL121 758 AL121758 AL121 766 AC005079 AC005079 AC005079 X99694 AFi 28444 AC0101 11 AF124518 AC004593 AC006907 Table 4 (continued) Mycobacteriumn avium strain GIR10 transcriptional regulator (may81) gene, Mycobactedriu aviumn partial cds, aconitase (acn), invasin 1 (invi), invasin 2 (inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG), enoyl-reductase (inhA) and ferrochelatase (mav272) genes, complete cds.

Propionib acteriu m freudenreichii hemY, hemH. hemB, hemnX. hemR and Propionibacterium hemi genes, complete cds- freudenreichii Homo sapiens clone NHO1 7201 3, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Homo sapiens clone NH1 172013, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Drosophila melanogaster chromosome 3UJ6261 clone RPCI98-1001 5, Drosophila melanogaste SEQUENCING IN PROGRESS 51 unordered pieces.

Corynebacterium glutamicumn gltB and gilD genes for glutamine 2- Corynebacterium oxoglutarate aminotransferase large and small subunits, complete cds. glutamicum Corynebacterium glutamicumn gltB and gIlD genes for glutamine 2- Corynebacterium oxoglutarate aminotransferase large and small subunits, complete cds. glulamicum EST229390 Normalized rat kidney. Benlo Soares Rattus sp. cDNA clone Rattus sp.

3Tend, mRNA sequence.

Homo sapiens chromosome 20 clone RP5-850E9, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Homo sapiens chromosome 20 clone RP5-850E9, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Human chromosome 14 DNA sequence **IN PROGRESS BAC R-412H8 Homo sapiens of RPCI-1 1 library from chromosome 14 of Homo sapiens (Human), complete sequence.

Homo sapiens clone RG252P22, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Homo sapiens clone RG252P322, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Homo sapiens clone RG252P22. SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Plasmid pEA3 nitrogen fixation genes. Enterobacter agglomera Rhodobacter capsulatus molybdenum cofactor biosynthetic gene cluster, Rhodobacter capsulatus partial sequence.

Drosophila melanogasler chromosome 3L170C1 clone RPCI98-9818. Drosophila melanogaste SEQUENCING IN PROGRESS 64 unordered pieces.

Corynebacterium glutamicum 3-dehydroquinase (aroD) and shikimate Corynebacterium dehydrogenase (aroE) genes, complete cds. glutamicum Homo sapiens PAC clone DJ0964C1 1 from 7p14-p15, complete sequence. Homo sapiens Caenorhabditis elegans clone Y76B1 2. SEQUENCING IN PROGRESS Caenorhabditis elegans unordered pieces.

r 46,667 37,451 37,451 38,627 92,113 93,702 34,221 37,965 37.965 38,796 38,227 38,227 38,227 48,826 40,135 39,527 98,237 36,616 37,095 6-Feb-99 21 -Aug-99 21 -Aug-99 16-OCT- 1999 13-MAR- 1999 1 3-MAR- 1999 31-Jan-99 03-DEC.

1999 03-DEC- 1999 11 -Nov-99 22-Nov-98 22-Nov-98 22-Nov-98 2-Aug-96 22-MAR- 1999 16-OCT.

'1999 18-MAY.

1999 18-Apr-98 26-Feb-99 54,191 26-MAR- 1996 ns rxaOO241 1826 GBBA1:.CGLYSI 4232 X60312 C.glutamicumn lysI gene for L-lysine perrnease.

Corynebacterium glutamicum 100,000 30-Jan-92 2006200807 24 Feb 2006 GBHrG1:PFMALI3P 192581 AL049180 GB13-ITG1:PFMAL13P 192581 AL049180 GB_1N2:EHU89655 3219 U89655 GB_1N2:EHU89655 3219 U89655 Table 4 (continued) Plasmodium falciparumn chromosome 13 strain 3D7, SEQUENCING IN PROGRESS in unordered pieces.

Plasmodium falciparumn chromosome 13 strain 3037. SEQUENCING IN PROGRESS *.in unordered pieces.

Entamoeba histolytica unconventional myosin lB mRNA. complete cdls.

Entamoeba histolytica unconventional myosin 11B mRNA, complete cds.

rxa00262 1197 rxa00266 531 =x00278 1155 rxa00295 1125 rxa00323 1461 rxa00324 3258 rxaOO330 1566 rxa00335 1554 GB RO:AF016190 2939 EMPAT:E09719 3505 AF016190 M us muscul us con nexin-36 (Cx36) gene, complete cds.

E0971 9 DNA encoding precursor protein of alkaline cellulase.

GBPATE021133 GBIN1:CELKO5F6 GB-BA1 :CGU43535 GBRO:RNU30789 GBBA2:CGU31281 GBBA1:BRLBIOBA GBPAT:E03937 GBBA1:MTCY427 GBBA1:MSGB32CS GB-BA1 :MTCY427 GBBA1:MSGB32CS GBBA1:MTCY427

GBOM:BOVELA

GBBA1:CGTHRC GBPAT:109078 GB-BA1 :BLTHRESY

N

GBBA1:CGGLNA 3494 36912 2531 3510 1614 1647 1005 38110 36404 38110 36404 38110 3242 3120 3146 1892 3686 E02133 AF040653 U43535 U30789 U31281 014084 E03937 Z70692 L78818 Z70692 L78818 Z70692 J02717 X56037 109078 Z29563 Y13221 gONA encoding alkaline cellulase.

Caenorhabditis elegans cosmid K05F5.

Corynebacterium glutamicumn multidrug resistance protein (cmr) gene, complete cds.

Rattus norvegicus clone N27 mnRNA.

Corynebacteriuni glutamicumn biotin synthase (bloB) gene, complete cds.

Brevibacteriurn flavumn gene for biotin synthetase, complete cdls.

DNA sequence encoding Brevibacterium flavum biotin-synthase.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 99/162.

Mycobacteriumn leprae cosmid 632 DNA sequence.

Mycobacterium tuberculosis ll37Rv complete genome; segment 99/162.

Mycobacteriumn Ieprae cosmid 832 DNA sequence.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 99/1 62.

Bovine elastin a mRNA, complete cds.

Corynebacterium glutamnicumn thrC gene for tlireonine synthase (EC 4.2.99.2) Sequence 4 from Patent WO 8809819.

Brevibacteriumn lactofermentum; ATCC 13869;; DNA (genomic);.

Corynebacterium glutamnicumn gInA gene.

Plasmodiumn falciparumn Plasmodium falclparurn Entamoeba histolylica Entamoeba histolytica Mus musculus Bacillus sp.

Bacillus sp.

Caenorhabditis elegans Corynebacterium glutamicum Rattus norvegicus Corynebacterium glutamicum Corynebacterium glutamicumn Corynebacterium glutamnicumn Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium tuberculosis Bos taurus Corynebacterium glutamicum Unknown.

Corynebacterium glutamicum Corynebacterium glutamicum 34,947 11-Aug-99 34,947 11 -Aug-99 36,496 23-MAY- 1997 37,544 23-MAY- 1997 41,856 9-Feb-99 34,741 08-CT- 1997 (Rel.

52, Created) 34,741 29-Sep-97 36,943 6-Jan-98 36,658 9-Apr-97 38,190 20-Aug-96 99,111 21-Nov-96 98,489 3-Feb-99 98,207 29-Sep-97 35,615 24-Jun-99 60,917 15-Jun-96 44,606 24-Jun-99 52,516 15-Jun-96 38,079 24-Jun-99 39,351 27-Apr.93 99,808 17-Jun-97 99,617 02-DEC- 1994 99,170 20-Sep-95 100,000 28-Aug-97 2006200807 24 Feb 2006 rxa00347 891 GBBA2:AF005635 1690 GBBAl :MSGB27CS 38793 GBEST27:A1455217 624 GB_BA2:SSU30252 2891 GB-EST21:AA9I 1262 581 rxa00351 1578 rxa00365 727 rxa00366 480 rxaCO367 4653 rxa00371 1917 GB-BA1:MLU1 5187 GBJIN2:AC004373 GB_1N2:AF145653 GBBA1 :AB024 708 GB-BA1 :MTCY1A6 GBBA1:SC3A3 GB-BA1 :AB024708 GBBA1:MTCY1A6 GB-BA1 :SC3A3 GBBAl :AB024708 GBBA1:MTCY1A6 GB-BA1 :SC3A3

GBVI:SBVORFS

GBEST37AI967505 36138 72722 3197 8734 37751 15901 8734 37751 15901 8734 37751 15901 7568 380 Table 4 (continued) AF005635 Corynebacterium glutamicum glutamine synthetase (gInA) gene, complete cds.

L78817 Mycobacterium leprae cosmid 827 DNA sequence.

A1455217 LD21828.3prime LD Drosophila melanogaster embryo pOT2 Drosophila melanogaster cDNA clone LD21828 3prime, mRNA sequence.

U30252 Synechococcus PCC7942 nucleoside diphosphate kinase and ORF2 protein genes, complete cds, ORFi protein gene, partial cds. and neutral site I for vector use.

AA911262 oe75a02.sl NCI_-CGAP_-Lu5 Homo sapiens cDNA clone IMAGE:1417418 3' similar to gb:A18757 UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR, GPI-ANCHORED (HUMAN);, mRNA sequence.

U15187 Mycobacterium leprae cosmid L296.

AC0D4373 Drosophila melanogaster DNA sequence (P1 DS05273 complete sequence.

AF145653 Drosophila melanogaster clone GH08860 BcDNA.GH08860 (BcDNA.GH08860) mnRNA, complete cds.

A8024708 Corynebacterium glutamicum gltB and gltD genes for glutamnine 2oxoglutarate aminotransferase large and small subunits, complete cds.

Z83864 Mycobacteriumn tuberculosis H37Rv complete genomne: segment 159/162.

AL1 09849 Streptomyces coelicolor cosmid 3A3.

AB024708 Corynebacterium glutamnicum gltB and gltD genes for glutamine 2oxoglutarate amninotransferase large and small subunits, complete cds.

Z83864 Mycobacterium tuberculosis H37Rv complete genome; segment 159/162.

ALl109849 Streptomnyces coelicolor cosmid 3A3.

AB024708 Corynebacterium glutamicun, gIB and gilD genes for glutamine 2oxoglutarate aminotransferase large and small subunits, complete cds.

Z83864 Mycobacterium tuberculosis H37Rv complete genome; segment 159/162.

AL1 09849 Streptomyces coelicolor cosmid 3A3.

M89923 Sugarcane bacilliform virus ORF 1 ,2,and 3 DNA, complete cds.

A1967505 Ljirnpesto3-215-cIO Ljirnp Lambda Hybrilap two-hybrid library Lotus japonicus cDNA clone LP215-03-clO 5' similar to 60S ribosomal protein L39, mRNA sequence.

AF043700 Caenorhabditis elegans cosmid K09H9.

Corynebacterium glutamicum Mycobacterium leprae Drosophila melanogaster Synechococcus PCC7942 Homo sapiens Mycobacterium leprae Drosophila melanogaster Drosophila melanogaster Corynebacterium glutamicum Mycobacterium tuberculosis Streptomyces coelicolor A3(2) Corynebacterium glutamicum Mycobacterium tuberculosis Streptomyces coelicolor A3(2) Corynebacteriumn glutamicum Mycobacterium tuberculosis Streptomyces coelicolor A3(2) 98.906 14-Jun-99 66.345 15-Jun-96 34,510 09-MAR- 1999 37,084 29-OCT- 1999 37,500 21-Apr-98 52,972 46,341 49,471 96,556 39,496 37,946 99,374 41,.333 37,554 99,312 36,971 37,905 09-MAR- 1995 17-Jul-98 14-Jun-99 13-MAR- 1999 17-Jun-98 16-Aug-99 13-MAR- 1999 17-Jun-98 16-Aug-99 13-MAR- 1999 17-Jun-98 16-Aug-99 12-Jun-93 24-Aug-99 Sugarcane bacilliform virus 35,843 Lotus japonicus 42,593 GB-IN1:CELK09H9 37881 Caenorhabditis elegans 34,295 22-Jan-98 2006200807 24 Feb 2006 rxaOO377 1245 rxa00382 1425 rxa00383 1467 rxaOO391 843 rxa00393 1017 rxaOO402 623 rxa00403 1254 GBBA1:CCU13664 GBPL1:ANSOGENE GBGSS4:AQ730303

GBBAI:PAHEML

GBBA1:MTY25D10 GBBA1:M5GY224 GBBA1:MLCBI222 GBHTG2:AC006269 GBHTG2:AC007638 GB EST38:AW1 705 3 GBPATAR065852 GB-V[:AF148805 GBBA1 :MTY25D1 0 GBBA1:MSGY224 GBBA1:MLB13O6 GB_8A2:AF052652 GBBA2:AF109162 GB_8A2:AF092918 GB BA2:AF052652 GBBA1:M1VO16 1678 1299 483 4444 40838 40051 34714 167171 178053 613 32207 28559 40838 40051 7762 2096 4514 20758 2096 53662 U13664 Y08866 AQ730303 X82072 Z95558 AD000004 AL049491 AC006269 AC007638 AW01 7053 AR065852 AF1 48805 Z95558 AD000004 Y13803 AF052652 AF109 162 AF092918 AF052652 AL02 1841 Table 4 (continued) Caulobacter crescentus uroporphyrinogen dlecarboxylase homolog (hemE) gene, partial cdls.

Anidlulans sD gene.

HS_-5505_-BI_-C04_T7A RPCI-1 1 Human Male BAC Library Homo sapiens genomic clone Plate=1081 Col=7 Row=F, genornic survey sequence.

P.aeruginosa hemL gene.

Mycobacterium tuberculosis H37Rv complete genome; segment 28/162.

Mycobacterium tuberculosis sequence from clone y224.

Mycobacterium leprae cosmid B31222.

Homo sapiens chromosome 17 clone hRPK.515 E23 map 17, SEQUENCING IN PROGRESS 2 ordered pieces.

Homo sapiens chromosome 17 clone hRPK.515 0 17 map 17, SEQUENCING IN PROGRESS 8 unordered pieces.

EST272398 Schistosoma mansoni male, Phil LoVerdle/Joe Merrick Schistosoma mansoni cDNA clone SMMAS 14 5 end, mRNA sequence.

Sequence 20 from patent US 5849564.

Kaposi's sarcoma-associated herpesvirus ORF 68 gene, partial cds: and ORF 69. kaposin, v-FLIP, v-cyclin, latent nuclear antigen, ORF K14, v-GPCR, putative phosphoribosylformylglycinamidine synthase, and LAMP (LAMP) genes, complete cds.

Mycobacterium tuberculosis ll37Rv complete genome; segment 28/162.

Mycobacterium tuberculosis sequence from clone y 22 4.

Mycobacterium leprae cosmid 81306 DNA.

Corynebacterium glutamicum homoserine 0-acetyltransferase (metA) gene, complete cds.

Corynebactenium dliphtheriae heme uptake locus, complete sequence.

Pseudomonas alcaligenes; outer membrane Xcp-secretion system gene cluster.

Corynebacterium glutamicum homoserine 0-acetytransferase (metA) gene.

complete cdls.

Mycobacterium tuberculosis ll37Rv complete genome; segment 143/162.

Caulobacter crescentus Emericella nidlulans Homo sapiens Pseudomonas aeruginosa Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Homo sapiens Homo sapiens Schistosoma mansoni Unknown.

Kaposi's sarcomaassociated herpesvirus Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Corynebacterium glutamicum Corynebacterium diphtheriae Pseudomonas alcaligenes Corynebacterium glutamicum Mycobacterium tuberculosis 36,832 39,603 36,728 54.175 61,143 61,143 43,981 35.444 34,821 40,472 38,586 38,509 36,308 39,282 39,228 99,672 40,830 50,161 99,920 52,898 24-MAR- 1995 1 7-OCT- 1996 15-Jul-99 1 8-DEC- 1995 17-Jun-98 03-DEC- 1996 27-Aug-99 10D-Jun-99 22-MAY- 1999 10-Sep-99 29-Sep-99 2-Aug-99 17-Jun-98 03-DEC- 1996 24-Jun-97 19-MAR- 1998 8-Jun-99 06-DEC- 1998 19-MAR- 1998 23-Jun-99 GB-EST23:AI1 11288 750 A1111288 SWOvAMCAQO2AO5SK Onchocerca volvulus adult male cONA (SAW98MLW-Onchocerca volvulus OvAM) Onchocerca volvulus cONA clone SWOvAMCAQO2AO5 5, mRNA sequence.

37,565 31-Aug-98 2006200807 24 Feb 2006 rxaOO4OS 613 rxa00420 1587 rxa00435 1296 rxa00437 579 rxa00439 591 rxa00440 582 ncaOO44l 1287 rxaOO446 987 GBBA1:MTVO16 GBPR4:AC005145 GB-BA1 :MTVO1 6 GBBA1:MTY13D12 GBBA1:MSGY126 GB BA1:MSGB971C

S

GB-BA1 :AFAC8BTZ GBHTG4:AC009541 GB-HTG4:AC009541 GBPR4:AC005951 GBBA1:SC2A11 GBPR4:AC005951 GBBA1:MTVO16 GBPL2:AF167358 GB-HTG3:AC0091 20 GBBA2:SKZ861 11 GBBA1:SC2E1 GB_8A1:SC2E1 GB-PR2:HS173D1 53662 143678 53662 37085 37164 37566 2760 169583 169583 155450 22789 155450 53662 1022 269445 7860 38962 38962 117338 AL021 841 AC005145 AL02 1841 Z80343 AD00001 2 L78821 M68904 AC009541 AC009541 AC005951 AL031 184 AC005951 AL021 841 AF1 67358 AC009120 Z861 11 AL023797 AL023797 AL031 984 AL1 09931 AL1 09931 AL034355 AC009367 AC009367 Table 4 (continued) Mycobacteriumn tuberculosis H37Rv complete genome: segment 143/162 Mycobacterium tuberculosis Homo sapiens Xp22-1 68-1 69 GSHB-523A23 (Genome Systems Human BAC Homo sapiens library) complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 143/162.

Mycobacterium tuberculosis H-37Rv complete genome; segment 156/1 62.

Mycobacterium tuberculosis sequence from clone y126.

Mycobacteriumn leprae cosmid B971 DNA sequence.

Alcaligenes eutrophus chromsomal transketolase (cbbTc) and phosphoglycolate phosphatase (cbbZc) genes, complete cds.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS ~,25 unordered pieces.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS ~,25 unordered pieces.

Homo sapiens chromosome 17. dlone hRPK.372_K_20. complete sequence.

Streptomyces coelicolor cosmid 2A1 1.

Homo sapiens chromosome 17, clone hRPK.372_K_20, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 1431162.

Rumex acetosa expansin (EXP3) gene, partial cds.

Homo sapiens chromosome 16 done RPCI-1 1_484E3.,* SEQUENCING IN PROGRESS 34 unordered pieces.

Streptomyces lividans rpsP. trmD, rplS, sipW, sipX, sipY, sipZ, mutT genes and 4 open reading frames.

Streptomyces coelicolor cosmid 2E1.

Streptomyces coelicolor cosmid 2E1.

Human DNA sequence from clone 17301 on chromosome 1p36.21- 36.33.Contains ESTs, STSs and GSSs, complete sequence.

Homo sapiens chromosome X clone RP4-719K3 map q21.1-21.31, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome X clone RP4-719K3 map q21.1-21.31, SEQUENCING IN PROGRESS in unordered pieces.

Streptomyces coelicolor cosmid D78.

Drosophila melanogaster chromosome 3L/76A2 clone RPCI98-48BI 5, SEQUENCING IN PROGRESS 44 unordered pieces.

Drosophila melanogaster chromosome 31J76A2 clone RPCI98-48B 5.

SEQUENCING IN PROGRESS 44 unordered pieces.

Mycobacteriumn tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium Ieprae Ralstonia eutropha Homo sapiens Homo sapiens Homo sapiens Streptomyces coelicolor Homo sapiens Mycobacterium tuberculosis Rumex acetosa Homo sapiens Streptomyces lividans Streptomyces coelicolor Streptomyces coelicolor Homo sapiens Homo sapiens Homo sapiens Streptomyces coelicolor Drosophila melanogaster Drosophila melanogaster 57,259 34,179 40,169 62,031 61,902 39,651 38,677 36,335 36,335 31,738 43,262 37,647 37,088 46,538 43.276 43,080 42,931 36,702 38,027 34,521 34,521 56,410 34,959 34,959 23-Jun-99 08-DEC- 1998 23-Jun-99 17-Jun-98 lO-DEC- 1996 15-Jun-96 27-Jul-94 1 2-OCT- 1999 1 2-OCT- 1999 18-Nov-98 5-Aug-98 18-Nov-98 23-Jun-99 17-Aug-99 3-Aug-99 27-OCT- 1999 4-Jun-98 4-Jun-98 23-Nov-99 03-DEC- 1999 03-DEC- 1999 26-Nov-98 16-OCT- 1999 16-OCT- 1999 GBHTG2:HSDJ719K 267114 3 GB-HTG2:HSDJ719K 267114 3 GBBA1:5CD78 36224 GB-HTG4:ACC09367 226055 GBHTG4:AC009367 226055 2006200807 24 Feb 2006 rxa00448 1143 rxa00450 424 GBPR3:AC003670 GB-HTG2:AF029367 GBHTG2:AF029367 GB-HTG2;AC007824 88945 148676 148676 133361 AC003670 AF029367 AF029367 AC007824 Table 4 (continued) Homo sapiens 12q13.1 PAC RPCI1-130F5 (Roswell Park Cancer Institute Homo sapiens Human PAC library) complete sequence.

Homo sapiens chromosome 12 clone RPCI-l 130F5 map 12q1 3. 1, Homo sapiens SEQUENCING IN PROGRESS 156 unordered pieces.

Homo sapiens chromosome 12 clone RPCI-I 130F5 map 12q1 3. 1, Homo sapiens SEQUENCING IN PROGRESS'-, 156 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACR0216 (0715) RPCI-98 Drosophila mel 02.1.16 map 89E-90A strain y; cn bw sp, **SEQUENCING IN PROGRESS 91 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACR02LI16 (0715) RPCI-98 Drosophila mel 02.1.16 map 89E-90A strain y; cn bw sp, -SEQUENCING IN PROGRESS -,91 unordered pieces.

wkl4aO8.xl NCI_CGAPLym12 Homo sapiens cDNA clone IMAGE:24 12278 Homo sapiens 3'similar to gb:Y00764 UBIQUINOL-CYTOCHROME C REDUCTASE I11 KID PROTEIN (HUMAN);, mRNA sequence.

Mycobacterium Ieprae cosmid B1779. Mycobacteriurm Drosophila melanogaster cosmid clone 86E4. Drosophila mel 927P1-2H3.TP 927PI Trypanosoma brucei genomic clone 927P1-2H3, Trypanosomat genomic survey sequence.

anogaster anogaster 35,682 31.373 31.373 40,000 40,000 9-Jun-98 18-OCT- 1997 I 8-OCT- 1997 2-Aug-99 2-Aug-99 GBHTG2:AC007824 133361 AC007824 GBE5T35:A1818057 412 A1818057 35,714 24-Aug-99 rxaOO46l 975 GBBA1:MLCB1779 43254 GB-IN1 :UMC86E4 29352 GBG5515:A064032 467 rxa00465 rxa00487 1692 GB_BA1:BAGUAA 3866 GB_BA2:U00015 42325 GBBAl :MTCY78 33818 rxa00488 1641 GBBA1:MTCY78 33818 GB_BA2:U00015 42325 GB -BA1 :SCAJ1O6O1 4692 rxa00489 1245 GB_BA2:U00015 42325 GB-HTG2:HS225E1 2 126464 GBHTG2:H5225E12 126464 rxa00533 1155 GBBAl :CGLYS 2803 Z98271 AL021 086 AQ640325 leprae anogaster rucei 39,308 37,487 38,116 8-Aug-97 27-Apr-99 8-Jul-99 Y10499 UOOO15 Z77165 Z77165 U00015 AJO1 0601 UOOO15 AL031 772 AL031 772 X57226 B.ammoniagenes guaA gene.

Mycobacterium leprae cosmid B1620.

Mycobacterium tuberculosis H37Rv complete genome; segment 145/162.

Mycobacterium tuberculosis H37Rv complete genome; segment 145/1 62.

Mycobacterium leprae cosmid 81620.

Streptomyces coelicolor A3(2) DNA for whiD and whiK loci.

Mycobacterium leprae cosmid 81620.

Homo sapiens chromosome 6 clone RP1 -225E1 2 map q24, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 6 clone RP 1-225E 12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

C. glutamnicumn lysC-alpha. IysC-beta and asd genes for aspartokinase-alpha and -beta subunits, and aspartate beta semnialdehyde dehydrogenase, respectively (EC 2.7.2.4; EC 1.2.1.11).

Corynebacterium ammoniagenes Mycobacteriumn leprae Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium Ieprae Streptomyces coelicolor Mycobacterium leprae Homo sapiens Homo sapiens Corynebacterium glutamicum 74,259 37,248 39.725 39,451 39,178 60,835 38,041 36,756 36,756 99,913 8-Jan-98 01-MAR- 1994un9 17-Jun-98 01-MAR- 1994 1 7-Sep-98 01-MAR- 1994 03-DEC- 1999 03-DEC- 1999 1 7-Feb-97 2006200807 24 Feb 2006 Table 4 (continued) X82928 C.glutamicum aspartate-semialdehyde dehydrogenase gene. GBBA1:CGCYSCAS 1591

D

rxa00534 1386 rxa00536 1494 rxa00537 2409 rxaOO54l 792 rxaOO558 1470 rxa00579 1983 rxaOO58O 1425 GBPAT:A07546 GB-BA1 :CGLYS GB-BA1:CORASKD GBPAT:E14514 GB-BA1 :CGLEUA GBBA1:MTVO25 GBBA1:MTU88526 GBBA2:S0D25 GBBA1:MTCY7H7A GBBA1:MTU34956 GB-PAT:192052 GB-BA1 :MTCY369

GB-BAI:BAPURF

GBBA1:MLU15182 GB-BA1 :MTCY7H7A GB-PAT:AR016483 EMPAT:E1 1273 GBPAT:E1 2594 GB_PAT:E12594 2112 A07546 Recombinant DNA fragment (Pst[-Xhol).

2803 X57226 C. glutamicum tysC-alpha, lysO-beta and asd genes for aspartokinase-aipha and -beta subunits, and aspartate beta semnialdehyde dehydrogenase, respectively (EC 2.7.2.4; EC 1.2. 1.11).

2957 L16848 Corynebacterium flavumn aspartokinase (ask), and aspartate-semialdehyde dehydrogenase (asd) genes, complete cds.

1643 E14514 DNA encoding Brevibacterium aspartokinase.

3492 X70959 C.glutamicum gene leuA for isopropylmalate synthase.

121125 AL022121 Mycobacterium tuberculosis H37Rv complete genome: segment 155/162.

2412 U88526 Mycobacterium tuberculosis putative alpha-isopropyt malate synthase (leuA) gene, complete cds.

41622 AL1 18514 Streptomyces coelicolor cosmid 025.

10451 Z9561 6 Mycobacterium tuberculosis H-37Rv complete genome; segment 39/162.

2462 U34956 Mycobacterium tuberculosis phosphoribosylformylglycinamidine synthase (putL) gene, complete cds.

2115 192052 Sequence 19 from patent US 5726299.

38109 Z95151 Mycobacterium Ieprae cosmid B5.

36850 Z80226 Mycobacterium tuberculosis H37Rv complete genome; segment 36/1162.

1885 X91252 Bammoniagenes purF gene.

40123 U15182 Mycobacterium leprae cosmid B2266.

10451 Z95618 Mycobacterium tuberculosis H37Rv complete genome, segment 39/162.

2104 AR016483 Sequence 1 from patent US 5776740.

2104 E11273 DNA encoding serine hydroxymethyl transferase.

Corynebacterium glutamicum synthetic construct Corynebactenium glutamicum Corynebacterium flavescens Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium tuberculosis Streptomyces coelicolor A3(2) Mycobacterium tuberculosis Mycobacterium tuberculosis Unknown.

Mycobacterium leprae Mycobacterium tuberculosis Corynebacterium ammoniagenes Mycobacterium leprae Mycobacterium tuberculosis Unknown.

Corynebacterium glutamicum Corynebacerium glutamicum Corynebacterium glutamicum 99,221 99,391 99.856 98,701 98.773 100,000 68,003 68,185 63,187 62,401 62,205 98,359 62,468 60,814 66.095 64,315 64,863 98,810 98,810 98.810 99,368 17-Feb-97 30-Jul-93 1 7-Feb-97 11I-Jun-93 28-Jul-99 10-Feb-99 24-Jun-99 26-Feb-97 21-Sep-99 17-Jun-98 28-Jan-97 0l-DEC- 1998 24-Jun-97 17-Jun-98 5-Jun-97 09-MAR- 1995 17-Jun-98

OS-DEC-

1998 08-OCr- 1997 (Rel.

52, Created) 24-Jun-98 24-Jun-98 2104 E12594 2104 E12594 DNA encoding serine hydroxymetlryltransferase from Brevibacterium flavumn.

DNA encoding serine hydroxymethyltransferase from Brevibacterium flavumn.

2006200807 24 Feb 2006 ncaOO58l 1092 rxa00584 1248 rxa00618 1230 rxa0061 9 1551 rxa00620 1014 rxa00624 810 rxa00626 1386 GBPAT:AR016483 2104 EMPAT:E1 1273 2104 GBPAT:E12594 2 104 EMPAT:E11273 2104 GBPAT:AR016483 2104 GBBA1:CORAHPS 2570 GBBAI:AOPCZA361 37941 GB-BA1:D90714 14358 GBEST19:AA8O2737 280 GB EST28:A1534381 581 AR01 6483 E11273 E12594 E11273 AR01 6463 L07603 AJ223998 D90714 AA802737 A1534381 X89858 Z80226 Z95 151 A60305 AF053247 M91546 AC008763 Z95559 AA362167 Z95559 Z80226 Z95151 U 15187 Table 4 (continued) Sequence 1 from patent US 5776740. Unknown.

DNA encoding serine hydroxymethyl transferase. Corynebacterium glutamicum DNA encoding serine hydroxymethyltransferase from Brevibacterium flavum. Corynebacterium glutamicum DNA encoding serine hydroxymethyl transferase. Corynebacterium glutamicum Sequence 1 from patent US 5776740. Unknown.

Corynebacterium glutamicum 3-deoxy-D-arfabinoheptufosonate-7-phosp hate Corynebacteriumn synthase gene, complete cds. glutamicum Amycolatopsis orientalis cosmid PCZA361. Amycolatopsis. orientalis Escherichia coli genomic DNA. (16.8 17.1 min). Escherichia coli GMO6236.5prime GM Drosophila melanogaster ovary BlueScript Drosophila Drosophila melanogaster melanogaster cDNA clone GM06236 5prime. mRNA sequence.

SD07186.5prime SD Drosophila melanogaster Schneider L2 cell culture pOT2 Drosophila melanogaster Drosophila melanogaster cDNA clone SDO71 86 5prime similar to X89858: Ani FBgnO0l 1558 PID:g927407 SPTREMBL:Q24240, mRNA sequence.

O~melanogaster mRNA for anillin protein. Drosophila melanogaster Mycobacterium tuberculosis H37Rv complete genome; segment 36/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B5. Mycobacterium leprae Sequence 5 from Patent W09708323- unidentified Pneumocystis carinii f. sp. ratti enolase mRNA, complete cds. Pneumocystis cariniii f. sp.

ratti Streptomyces lividans amninopeptidase P (PepP) gene, complete cds. Streptomyces lividans Homo sapiens chromosome 19 clone CITB-E1_3214H19, SEQUENCING Homo sapiens IN PROGRESS unordered pieces.

Caenorhabditis elegans cosmid Y41E3, complete sequence. Caenorhabditis elegans EST71561 Macrophage I Homo sapiens cDNA V'end, mRNA sequence. Homo sapiens Caenorhabditis elegans cosmid Y41E3, complete sequence. Caenorhabditis elegans Mycobacterium tuberculosis H37Rv complete genome; segment 36/162. Mycobacterium tuberculosis Mycobacterium Ieprae cosmid B5. Mycobacterium leprae Mycobacteriumn leprae cosmid L296. Mycobacterium leprae 99,368 99.368 37,071 37,071 37,071 98,236 54,553 53,312 39,928 41,136 34,398 62,776 61.831 61,785 41,060 37,126 40,020 36,986 38.378 37,694 57,971 58,806 38.007 1998 08-OCT- 1997 (Rel.

52, Created) 24-Jun-98 08-OCT- 1997 (Rel.

52, Created)

OS-DEC-

1998 26-Apr-93 29-MAR- 1999 7-Feb-99 25-Nov-98 1 8-MAR- 1999

P

8-Nov-95 17-Jun-98 24-Jun-97 06-MAR- 1998 5-Jan-99 12-Jun-93 3-Aug-99 2-Sep-99 21-Apr-97 2-Sep-99 17-Jun-98 24-Jun-97 09-MAR- 1995 GBIN1:DMANILLIN GBBAI:MTCY369 GBPAT:A60305 GBPL2:AF063247 GBBA1:STMAPP GBHTG3:AC008763 GBIN1:CEY41E3 GBEST13:AA362167 GBIN1:CEY41E3 GBBA1:MTCY369 GBBA1:MLCBS GB-BA1 :MLU1 5187 4029 36850 38109 1845 1450 2069 214575 150641 372 150641 36850 38109 36138 2006200807 24 Feb 2006 rxa00632 795 rxa00633 1392 rxa00688 666 rxaOO708 930 rxaOO717 1083 rxa00718 831 rxa00727 1035 GB-BA1 :BRLB lOAD GBPAT:E04041 GB_PAT:E04040 GBBA1:BRLBIOAD GBPAT:E04040 GB3BA2:EHU3851 9 GBBA1:MTVO41

GB_BAI:BRLSECY

GB-BA2:MBU77912 GB-BA2:AF1 57493 GBPAT:100836 GB-PAT:E0031 1 GB-PAT:178753 GB-PAT:192042 GBBA1:MTCI125 GB-BAI MTCI1125 GBBA1:MTCI125 GB-GSS12:AQ42075 GBHTG3:AC008332 2272 675 1272 2272 1272 1290 28826 1516 7163 25454 1853 1853 1187 1187 37432 37432 37432 671 118545 D14083 E04041 E04040 014083 E04040 U38519 AL021958 D14162 U77912 AF1 57493 100836 E00311 178753 192042 Z98268 Z98268 Z98268 A0420755 AC008332 Table 4 (continued) Brevibacterium flavum genes for 7,8-diaminopelargonic acid aminotransferase Corynebacterium and dlethiobiotin synthetase. complete cdls. glutamicum DNA sequence coding for desthiobiotinsynthetase. Corynebacterium glutamicum DNA sequence coding for diamino pelargonic acid aminotransfe rase. Corynebacterium glutamicum Brevibacterium flavumn genes for 7.8-diaminopelargonic acid aminotransferase Corynebacterium and dethiobiotin synthetase, complete cdls. glutamicum DNA sequence coding for diamino pelargonic acid aminotransferase. Corynebacterium glutamicum Erwinia herbicola adenosylmethionine-8-amino-7-oxononanoate transaminase Erwinia herbicola (bioA) gene, complete cdls.

Mycobacterium tuberculosis H37Rv complete genome; segment 35/1 62. Mycobacterium tuberculosis Brevibacterium flavum gene for SecY protein (complete cds) and gene or Corynebacterium adenylate kinase (partial cds). glutamicumn Mycobacterium bovis MBE5Oa gene, partial cdls: and MBE5Ob, MBE5Oc. Mycobacterium bovis preprotein translocase SecY subunit (secY), adenylate kinase (adk), methionine aminopeptidase (map), RNA polymerase ECF sigma factor MBE50d. and MBE5Oe genes, complete cdls.

Zynromonas mobilis ZM4 fosmid clone 42137, complete sequence. Zymomonas mobilis Sequence 1 from Patent US 4758514. Unknown.

DNA coding of 2,5-diketogluconic acid reductase. unidentified Sequence g from patent US 5693781. Unknown.

Sequence 9 from patent US 5726299. Unknown.

Mycobacterium tuberculosis H37Rv complete genome: segment 76/162. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 76/1 62. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 76/1 62. Mycobacterium tuberculosis RPCI-1 1-168G1 8.TJ RPCI-1 1 Homno sapiens genomic clone RPCI-1 1 Homo sapiens 168G 18, genomic survey sequence.

Drosophila melanogaster chromosome 2 clone BACR4801 0 (D867) RPCI-98 Drosophila melanogas 48.03.10 map 34A-34A strain y; or bw sp, **SEQUENCING IN PROGRESS 78 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR48D1 0 (0867) RPCI-98 Drosophila melanogas 48.D.10 map 34A-34A strain y; cn bw sp, SEQUENCING IN PROGRESS-. 78 unordered pieces.

97,358 98,074 93,814 95,690 95.755 55,564 60,030 99.563 60,030 39,116 47.419 47,419 37,814 37,814 50,647 55,228 40,300 35.750 40,634 40.634 3-Feb-99 29-Sep-97 29-Sep-97 3-Feb-99 29-Sep-97 4-Nov-96 17-Jun-98 3-Feb-99 27-Jan-99 5-Jul-99 21-MAY- 1993 29-Sep-97 3-Apr-98 01 -DEC- 1998 17-Jun-98 17-Jun-98 17-Jun-98 23-MAR- 1999 6-Aug-99 6-Aug-99 ~ter ~ter GB-HTG3:AC008332 118545 AC008332 2006200807 24 Feb 2006 GB-HTG3:AC008332 118545 AC008332 rxa00766 966 rxa00770 1293 rxa00779 1056 rxaOO780 669 rxa00838 1023 rxa00863 867 rxa00864 873 GBHTG2:AC006769 GBHTG2:AC006789 GBBA1:D90810 GB-BA1 :MTV043 GBBA1:MLU15182 GBBA2:SCD25 GB-HTG1 :CERO8A5 GB-HTG1:CERO8A5 GBPL2:AF078693 GBBA1:MTCY98

GB-BAI:AVINIFREG

GBBA2:AF001780 GBESTI :Z30506 GBPL2:A0006258 GBEST37:A1998439 GBBA1:BUJAPAB GBPAT:E16749 GBPAT:E14520 GBBA1:BLDAPAB GBBA1:CGDAPB 83823 83823 20476 688348 40123 41822 51920 51920 1492 31225 7099 6701 329 110469 455 3572 2001 2001 3572 1902 AC006789 AC006789 09081 0 AL022004 U15182 ALl118514 Z82281 Z82281 AF078693 Z83860 M60090 AF001 780 Z30506 AC006258 A1998439 Z21502 E16749 E14520 Z21502 X67737 Table 4 (continued) Drosophila melanogaster chromosome 2 clone BACR48D1O (D867) RPCI-98 Drosophila melanogaster 33,888 48.0.10 map 34A-34A strain y; cn bowsp, -SEQUENCING IN PROGRESS-, 78 unordered pieces.

Caenorhabditis elegans clone Y49F6, SEQUENCING IN PROGRESS ~,Caenorhaboditis elegans 36,737 2 unordered pieces.

Caenorhabditis elegans clone Y49F6, SEQUENCING IN PROGRESS ~,Caenorhabditis elegans 36.737 2 unordered pieces.

Ecoli genomic DNA, Kohara clone #319(37.4-37.8 min.). Escherichia col 36.526 Mycobacterium tuberculosis H37Rv complete genome; segment 40/1 62. Mycobacteriumn 66,193 tuberculosis Mycobacterium Ieprae cosmid B2266. Mycobacteriumn leprae 61,443 Streptomyces coelicolor cosmid D25. Streptomyces coelicolor 59.938 A3(2) Caenorhabditis elegans chromosome V clone R08A5, SEQUENCING IN Caenorhabditis elegans 64.896 PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome V clone R08A5, SEQUENCING IN Caenorhabditis elegans 64,896 PROGRESS in unordered pieces.

Chlamydomonas reinhardtii putative 0-acetylserine(thiol)lyase precursor Chlamydomonas reinhardtii 57,970 (Crcys-1A) mRNA, nuclear gene encoding organellar protein, complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 103/162. Mycobacteriumn 5.4,410 tuberculosis Azotobacter chroococcumn nifU, nifS, nifV, nifP, nifW, nifZ and nifM genes, Azotobacter chroococcumn 51,729 complete ods.

Cyanothece PCC 8801 NifP (nifP), nitrogenase (nifB), FdxN (fdxN), NifS (nifS) Cyanothece PCC8801 36,309 and NifU1 (nifU) genes, complete cds, and NitH (nifH) gene, partial cds.

ATTS2430 AC16H Arabidopsis. thaliana cIJNA clone TA1306 3, mRNA Arabidopsis thaliana 4,308 sequence.

Arabidopsis thaliana BAG F18G18 from chromosome V near 60.5 cM, Arabidopsis thaliana 35,571 complete sequence.

701545695 A. thaliana, Columbia Col-0, rosette-2 Arabidopsis thaliana cDNA Arabidopsis thaliana 36,044 clone 701545695, mRNA sequence.

Blactofermentum dapA and dapB genes for dihydrodipicolinate synthase and Corynebacterium 99,539 dihydrodipicolinate reductase. glutarniour gONA encoding dihydrodipicolinate synthase (DDPS)- Corynebacteriumn 99,539 g lutamnicumn DNA encoding Brevibacterium dihydrodipicolinic acid synthase. Corynebacterium 99.539 glutamicumn Blactofermentum dapA and dapB genes for dihydrodipicolinate synthase and Corynebactedriu 99,885 dihydrodipicolinate reductase. .glutarnicum Oglutamicum dapB gene for dihydrodipicolinate reductase. Corynebacteriumn 100.000 glutarnicurn 6-Aug-99 25-Feb-99 25-Feb-99 29-MAY- 1997 24-Jun-99 09-MAR- 1995 21-Sep-99 14-OCT- 1998 14-OCT- 1998 3-Nov-99 17-Jun-98 26-Apr-93 08-MAR- 1999 11-MAR- 1994 28-DEC- 1998 8-Sep-99 16-Aug-93 28-Jul-99 28-Jul-99 16-Aug-93 1-Apr-93 2006200807 24 Feb 2006 GBPAT:E14520 rxa00865 1026 GB-BA1:BLDAPAB GBPAr:E16752 GBPAT:AR038113 rxa00867 650 GBBAl :MTVOO2 GBBA1:MLCB22 GBBA1:SAU19858 rxa00873 779 GB-BA1:S00001206 GBBA1:SC0001205 GB-BA1 :D781 98 rxa00884 1263 GBBA1:MTCY253 GBBAl :MSGY222 GBGSSIS:AQ65460 0 rxaOO8Sl 1102 GBBA1:MTCI418B GB-BA1:SCODO 1206 GBBA1:SC0001205 rxa00952 963 EMPAT:E10963 2001 3572 1411 1411 56414 40281 2838 9184 9589 2304 4 1230 41156 468 11700 9184 9589 3118 7725 7725 7726 7725 7725 7726 E14520 Z21 502 E16752 AR038113 AL008967 Z98741 U19858 AJOG01206 AJO01 205 D78198 Z81 368 ADOOOOI 0 AQ654600 Z96071 AJO01 206 AJO01205 E10963 X04960 E01688 E01375 E01688 X04960 E01 375 Table 4 (continued) DNA encoding Brevibacterium dihydrodipicolinic acid synthase. Corynebacterium glutamicum Blactotermentum dapA and dapSl genes for dihydrodipicolinate synthase and Corynebacterium dihydrodipicolinate reductase. glutamicum gDNA encoding dihydrodipicolinate reductase (DDPR). Corynebacterium glutamicum Sequence 18 from patent US 5804414. Unknown.

Mycobacterium tuberculosis H37Rv complete genome: segment 1221162. Mycobacterium tuberculosis Mycobacterium Ieprae cosmid B22. Mycobacterium Ieprae Streptomyces antibioticus guanosine pentaphosphate synthetase (gpsl) gene. Streptomyces antibioticus complete ods.

Streptomyces coelicolor A3(2), glycogen metabolism cluster 11. Streptomyces coelicolor Streptomyces coelicolor A3(2) glycogen metabolism cluster. Streptomyces coelicolor Pimelobacter sp. DNA for trehalose synthase, complete cols. Pimelobacter sp.

Mycobacterium tuberculosis H37Rv complete genome; segment 106f162. Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone y222. Mycobacterium tuberculosis Sheared DNA-1014.TF Sheared DNA Trypanosoma brucei genomic clone Trypanosoma brucei Sheared DNA-1014, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 7/162. Mycobacterium tuberculosis Streptomyces coelicolor A3(2), glycogen metabolism cluster 11. Streptomyces coelicolor Streptomyces coelicolor A3(2) glycogen metabolism clusterl. Streptomyces coelicolor gDNA encoding tryptophan synthase. Corynebacterium glutamicum Brevibacterium lactofermentum tryptophan operon. Corynebacterium glutamicum Genomic DNA of trp operon of prepibacterium latophelmentamn. unidentified DNA sequence of tryptophan operon. Corynebacterium glutamicum Genomic DNA of trp operon of prepibacterium latophelmentamn. unidentified Brevibacterium lactofermentum tryptophan operon. Corynebacterium glutamicum DNA sequence of tryptophan operon. Corynebacterium glutamicum 100,000 100,000 99,805 99,805 39,179 39,482 69.706 63,415 61,617 60,594 37,785 38,006 33,974 63,297 61.965 61,727 99,688 98,847 98,428 98,758 98,758 98,758 98,372 28-Jul-99 16-Aug-93 28-Jul-99 29-Sep-99 17-Jun-98 22-Aug-97 1996 29-MAR- 1999 29-MAR- 1999 5-Feb-99 17-Jun-98 03-DEC- 1996 22-Jun-99 18-Jun-98 29-MAR- 1999 29-MAR- 1999 08-OCT- 1997 (Rel.

52, Created) 10O-Feb-99 29-Sep-97 29-Sep-97 29-Sep-97 10-Feb-99 29-Sep-97 rxa00954 644 rxaOO955 1545 GB,,BA1 :BLTRP GB-PAT:E0168 GB-PAT:EO1 375 GB,.PAT:EO1 688 GB-BA1 :BLTRP GBPAT:E01375 2006200807 24 Feb 2006 Table 4 (continued) 7725 X04960 Brevibactenium lactofermentum tryptophan operon. GB.BAI :BLTRP Corynebacteriumn rxa00956 1237 rxa00957 1677 rxa00958 747 rxaOO970 1050 rxa00972 1458 rxa00981 753 rxaOO9B9 1644 GB PATE01688 EM-PATE1 0963 GB-BA1 :BLTRP GBPAT:E01375 GB-BA1 :BLTRP GBPAT:E01375 GBPAT:E01688 GBBA1:BLTRP GBPAT:E01375 GB-PAT:E01688 GBBA1:CGH-OMTHR GBPAT:109077 GBPAT:E01358 GBPAT:E16755 GB PAT:AR0381 10 GBPAT:E14508 GBOV:GGA245664 GB-PL2:AC007887 GBGSS1 :CNSOORN w GBBAI:MTVOO8 GB-BAI :SCVALSFP GBBA1:MTVOO8 7725 E01688 3118 E 10963 7725 7726 7725 7726 7725 7725 7726 7725 3685 3685 2615 3579 3579 3579 512 159434 542 63033 3619 63033 X0496Q.

E01 375 X04960 E01 375 E01688 X04960 E01 375 E01688 Y00546 109077 E01358 E16755 AR0381 10 E14508 AJ245664 AC007887 AL087338 AL021 246 Y13070 AL021 246 glutamnicumn Genomic DNA of trp operon of prepibacterium latophelmentamn. unidentified gDNA encoding tryptophan synthase. Corynebacteriumn glutamicumn Brevibacterium lactofermentum tryptophan operon. Corynebacteriumn glutamicumn DNA sequence of tryptophan operon. Corynebacteriumn giutamicum Brevibacteriumn lactofermentumn tryptophan operon. Corynebacteriumn glutamicumn DNA sequence of tryptophan operon. Corynebacteriumn glutamicumn Genomic DNA of trp operon of prepibacteriumn Iatophelmentamn. unidentified Brevibacteriumn lactofermentumn tryptophan operon. Corynebacteriumn glutamicumn DNA sequence of tryptophan operon. Corynebacteriumn glutamicumn Genomic DNA of trp operon of prepibacteriumn Iatophelmentamn. unidentified Corynebacterium glutamicum hom-thrB genes for homoserine dehydrogenase Corynebacterium and homoserine kinase- glutamnicumn Sequence 1 from Patent WO 8809819. Unknown.

DNA encoding for homoserine dehydrogenase(HDH)and homoserine Corynebacteriumn kinase(HK). glutamicumn gDNA encoding diaminopimelate decarboxylase (DOC) and arginyl-tRNA Corynebacteriumn synthase. glutamicumn Sequence 15 from patent US 5804414. Unknown.

DNA encoding Brevibacterium diaminopimelic acid decarboxylase and arginyl- Corynebacterium tRNA synthase. glutamicumn Gallus gallus partial mRNA for ATP-citrate lyase (ACI gene). Gallus gallus Genomic sequence for Arabidopsis thaliana BAC F1 504 from chromosome 1, Arabidopsis thaliana complete sequence.

Arabidopsis thaliana genome survey sequence T7 end of BAC F14D7 of IGIF Arabidopsis thaliana library from strain Columbia of Arabidopsis thaliana, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 1081162. Mycobacteriumn tuberculosis S.coelicolor valS. fpgs. ndk genes. Streptomyces coelica Mycobacteriumn tuberculosis H37Rv complete genome; segment 108/162. Mycobacteriumn tuberculosis 98,372 98,242 98,949 99,107 98,945 99,165 98,927 98.867 98.792 98,792 98,658 99,905 99,810 97,524 99,931 99,931 99.931 37,538 37,600 41,264 40,773 58,119 38,167 10-Feb-99 29-Sep-97 08-OCT- 1997 (Rel.

52, Created) 10-Feb-99 29-Sep-97 10-Feb-99 29-Sep-97 29-Sep-97 10-Feb-99 29-Sep-97 29-Sep-97 12-Sep-93 02-DEC- 1994 29-Sep-97 28-Jul-99 29-Sep-99 28-Jul-99 28-Sep-99 04-OCT- 1999 28-Jun-99 ~lor 17-Jun-98 03-MAR- 1998 1 7-Jun-98 2006200807 24 Feb 2006 rxa00997 705 GB-BA2:CGU31225 1817 U31225 Table 4 (continued) Corynebacterium glutamicum L-proline:NADP+ 5-oxidoreductase (proC) gene. Corynebactenium complete cds. glutamicumn Caenortiabditis elegans chromosome IV clone Y39C12, SEQUENCING IN Caenortiabditis elegans GB-ITG1:CEY39C12 GB_INi:CEBOO1 rxa0l0l9 1110 GBHTG2:AC005052 GB-ITG2:AC005052 GBGSS9:AQ171808 rxa01026 1782 GBBAI:SC1C2 GB_BA1:ATLEUCD GBBAI:M1VOI2 rxa0lO27 1131 GB-BA1:MLCB537 GBBA1:MTCY349 GBBA1:SPUNGMUT x =010l73 954 GBBA1 :BACOUTB3 GB PR4:AC007938 GBPL2:ATA0006282 rXa0lO79 2226 GBBA2:AF1 12535 GBBA1:CANRDFGE

N

GBBA1:MTV012 rxa0lO80 567 GB-BA2:AF1 12535 GSBBA1 :CANRDFGE

N

GBBA1 :STNRD rxa0l 087 999 GB-IN2:AF063412 GB-PR3:HS24M1 5 GB1IN2:ARU85702 282838 39416 144734 '144734 512 42210 2982 70287 44882 43523 1172 1004 167237 92577 4363 6054 70287 4363 6054 4894 1093 134539 1240 AL009026 Z69634 AC005052 AC005052 AQ 1718C8 AL031 124 X84647 AL021 287 Z99263 Z83018 Z21 702 M1 5811 AC007938 AC006282 AF1 12535 Y09572 AL021 287 AF112535 Y09572 X73226 AF063412 Z94055 U85702 PROGRESS in unordered pieces.

Caenorhabditis elegans cosmid 80001, complete sequence.

H-omo sapiens clone RG038K21, SEQUENCING IN PROGRESS ~*3 unordered pieces.

Homo sapiens clone RG038K21, SEQUENCING IN PROGRESS ~.3 unordered pieces.

H5_31 79_Al_G03_T7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3179 Col=5 Row=M, genomic survey sequence.

Streptomyces coelicolor cosmid 1C2.

Ateichomyceticus leuC and leuD genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 1321162.

Mycobacterium Ieprae cosmid B637.

Mycobacterium tuberculosis H37Rv complete genome; segment 131/1 62.

S.pneumoniae ung gene and mutX genes encoding uracil-DNA glycosylase and 8-oxodGTP nucleoside triphosphatase.

Bacillus subtilis outB gene encoding a sporulation protein, complete cds.

Homo sapiens clone UWGC:djs2Ol from 7q31, complete sequence.

Arabidlopsis thaliana chromosome 11 SAC F13K3 genomic sequence, complete sequence.

Corynebacterium glutamicum putative glutaredoxin NrdH (nrdl-), NrdI (nrdl), and ribonucleotidle reductase alpha-chain (nrdE) genes, complete cds.

Corynebacteriumn ammoniagenes nrdH, nrdl, nrdE. nrdF genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 1321162.

Corynebacterium glutamicum putative glutaredoxin NrdH (nrdH), Nrdll (nrdl), and ribonucleotide reductase alpha-chain (nrdE) genes, complete eds.

Corynebactenium ammoniagenes nrdH, nrdl, nrdE, nrdF genes.

Sltyphmurium nrdEF operon.

Limnadia lenticularis elongation factor 1 -alpha mRNA, partial cdls.

Human DNA sequence from PAC 24M15 on chromosome 1. Contains tenascin-R (restrictin). EST.

Anathix ralla elongation factor-i alpha (EF-l a) gene, partial cds.

Haeomo apise a Homo sapiens Homo sapiens Streptomyces coelicolor Actinoplanes teichomyceticus Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium tuberculosis Streptococcus pneumoniae Bacillus subtilis Homo sapiens Arabidopsis thaliana Corynebacteriurn glulamicumn Corynebacterium ammoniagenes Mycobacterium tuberculosis Corynebacterium glutamicum Corynebacterium ammoniagenes Salmonella typhimuriumn Limnadia lenticularis Homo sapiens Anathix ralla 40,841 36,416 36,416 39,172 39.172 34,661 68,275 65,935 40,454 38,636 51,989 38,088 53.723 34,322 36,181 99,820 75,966 38,296 100,000 65,511 52,477 43,750 37,475 37,319 2-Aug-96 26-OCT- 1999 2-Sep-99 12-Jun-98 12-Jun-98 17-OCT- 1998 1 5-Jan-99 04-OCT- 1995 23-Jun-99 1 7-Sep-97 17-Jun-98 1 5-Jun-94 26-Apr-93 1-Jul-99 1 3-MAR- 1999 5-Aug-99 18-Apr-98 23-Jun-99 5-Aug-99 18-Apr-98 03-MAR- 1997 29-MAR- 1999.

23-Nov9 16-Jul-97 2006200807 24 Feb 2006 rxa0lO95 857 rxa0l 097 477 rxa0lO98 897 rxa011OO 861 GBBA1:MTCYO1B2 35938 Z95554 GBHTG5:AC011632 GBHTG5:ACO1 1632 GB-BA2:AF030405 GB-BA2:AF030405 GB-BA2:AF030405 GB-BA1 :MSGY223 GBBA1:MLCBI61O GBBA2;AF051846 175917 175917 774 774 774 42061 40055 738 GBBA2:AF060558 636 GB HTG1 :HSDJ14OA 221755 9 =01101~ 756 GB_8A2:AF060558 636 GBBA1:SC4G6 36917 GBBAl :STMHISOPA 3981 rxa01lO4 729 GBBAI:STMHISOPA 3981 GBBA1:SC4G6 36917 GB-BA1 :MTCY336 32437 1221 GBBA1:MTCY336 32437 GSBBA1 :MSGY223 42061 GBBA1:MLCB161O 40055 rxa0llO6 1449 GB-BA1:MSGY223 42061 AC01 1632 AC01 1632 AF030405 AF030405 AF030405 AD00001 9 AL04991 3 AF051 846 AF060558 AL109917 AF060558 AL096884 M31628 M31 628 AL096884 Z95586 Z95586 AD00001 9 AL04991 3 AD00001 9 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 72/162.

Homo sapiens clone RP1I1-3N1 3, WORKING DRAFT SEQUENCE, 9 unordered pieces.

Homo sapiens clone RP1 1-3N13, WORKING DRAFT SEQUENCE, 9 unordered pieces.

Corynebacterium glutamicum cyclase (his F) gene, complete cds.

Corynebacteriumn glLutamnicum cyclase (hisF) gene, complete cds.

Corynebacteriumn glutamicum cyclase (hisF) gene, complete cds.

Mycobacteriunm tuberculosis sequence from clone y223.

Mycobacterium Ieprae cosmid B1610.

Corynebacterium glutamicum phosphoribosylformimino.5-amino-1phosphoribosyl-4- imidazolecarboxamide isomerase (hisA) gene.

complete cdls.

Corynebacterium glutamnicumn glutamnine amidlotransfe rase (hisH) gene, complete cds.

Homo sapiens chromosome 1 clone RP1-140A9, **SEQUENCING IN PROGRESS in unordered pieces.

Corynebacterium glutamicum glutamnine amid otransferase (hisH) gene.

complete cds.

Streptomyces coelicolor cosmid 4G6.

S.coelicolor histidine biosynthesis operon encoding hisO, partial cds., and hisC, hisI3, hisH, and hisA genes, complete cdls.

S. coelicolor histidine biosynthesis operon encoding hisD, partial cdls., and hisC, hisB, hisH-, and hisA genes, complete cds.

Streptomyces coelicolor cosmid 4G6.

Mycobacterium tuberculosis H37Rv complete genome; segment 70/162.

Mycobacterium tuberculosis H-37Rv complete genome; segment 70/1 62.

Mycobacterium tuberculosis sequence from dlone y223.

Mycobacterium Ieprae cosmid 81610.

Mycobacterlum tuberculosis sequence from dlone y 22 3.

Mycobacterium tuberculosis Homo sapiens Homo sapiens Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium Ieprae Corynebacterium glutamicum Corynebacterium glutamicum Homo sapiens Corynebacterium glutamicum Streptomyces coelicolor A3(2) Streptomyces coelicolor Streptomyces coelicolor Streptomyces coelicolor A3(2) Mycobacteriumn tuberculosis Mycobacterium tuberculosis Mycobacteriumn tuberculosis Mycobacterium leprae Mycobacterium tuberculosis 43,243 36,471 36,836 100,000 41,206 97,933 40,972 61,.366 97,154 95,455 30,523 94,462 38,378 60,053 58,333 39,045 60,364 60,931 36,851 60,902 37,233 13-Nov-97 1996 27-Aug-99 12-MAR- 1998 29-Apr-98 23-Nov-99 29-Apr-98 23-Jul-99 26-Apr-93 26-Apr-93 23-Jul-99 24-Jun-99 24-Jun-99 lO-DEC- 1996 -:27-Aug-99 1O-DtC- 1996 17-Jun-98 19-Nov-99 19-Nov-99 13-Nov-97 13-Nov-97 2006200807 24 Feb 2006 GBBA1:MSHISCO GBBA1:MTCY336 rxa0ll45 1137 GBBAI:CORAIA GB-BA1 :BRLILVCA GBPAT:E08232 rxa0ll62 1449 GBPAT:A60299 GB-PR3:HS24ES GB PR3:AC005265 rxa01208 846 GB-HTG2:AC004965 GBHTG2:ACOO49S5 GB PL2:TAU55859 rxa0l2O9 1528 GBHTG3:ACO1 1469 GBHTG3:AC01 1469 GB-PL1 ABOl 0077 nxa01215 1098 GBBA1:MTCYIOG2 GBIN1:LEIPRPP GBHTG2:HSJ799D1 6 rxa01239 2556 GBBAI:MTCY48 GB PR2:AB029032 GB-GSS9:AQ107201 2298 X65542 Table 4 (continued) M.smegmatis genes hisD) and hisC for histidinol dehydrogenase and histidinol- Mycobacterium smegmatis 60,111 phosphate aminotransferase, respectively.

30-Jun-93 32437 4705 1364 1017 2869 35506 43900 323792 323792 2397 113436 113436 77380 38970 1887 130149 35377 6377 355 99923 99923 Z95586 L09232 D14551 E08232 A60299 Z82185 AC005265 AC004965 AC004965 U55859 AC01 1469 AC011469 AB010077 Z92539 M76553 AL05D344 Z74020 AB029032 AQ107201 AC005990 AC005990 Mycobacterium tuberculosis H37Rv complete genome; segment 70/1162.

Corynebacterium glutamicum acetohydroxy acid synthase (ilvB) and (ilvN) genes, and acetohydroxy acid isomeroreductase (ilvO) gene, complete cds.

Brevibacterium flavumn ilvC gene for acetohydroxy acid isomeroreductase, complete ods.

DNA encoding acetohydroxy-acid isomeroreductase.

Sequence 18 from Patent W09706261.

Human DNA sequence from Fosmid 24E5 on chromosome 22q1 1 .2-qler contains parvalbumin, ESTs, STS.

Homo sapiens chromosome 19, cosmid F1 9750, complete sequence.

Homo sapiens clone DJ1 1 061-14, SEQUENCING IN PROGRESS ~,42 unordered pieces.

Homo sapiens clone DJ1 1061-14, SEQUENCING IN PROGRESS ~,42 unordered pieces.

Triticum aestivumn heat shock protein 80 mRNA, complete cds..

Homo sapiens chromosome 19 clone CIT-HSPC 475D23, -SEQUENCING IN PROGRESS 31 unordered pieces.

Homo sapiens chromosome 19 clone CIT-HSPC_475D23, SEQUENCING IN PROGRESS 31 unordered pieces.

Arabidopsis thaliana genomic DNA, chromosome 5. P1 clone: MYHI 9, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 471162.' Leishmania donovani phosphoribosylpyrophosphate, synthetase gene.

complete ods.

Homo sapiens chromosome 1 clone RP4-799D16 map p34.3-36.1, SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 69/162.

Homo sapiens mRNA for KIAA1 109 protein, partial cds.

HS_3098_AlC3jT7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3098 Col=5 Row=-E, genomic survey sequence.

Arabidopsls thaliana chromosome 1 BAC F508 sequence, complete sequence.

Arabidopsis thaliana chromosome 1 BAC F508 sequence, complete sequence.

Mycobacterium tuberculosis Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Aspergillus niger Homo sapiens Homo sapiens Homo sapiens Homo sapiens Triticumn aestivumn H-omo sapiens H-omo sapiens Arabidopsis; thaliana Mycobacterium tuberculosis Leishmania donovani Homo sapiens Mycobacterium tuberculosis Homo sapiens Homo sapiens Arabidopsis thaliana Arabidopsis thaliana 58,420 24-Jun-99 100.000 99,560 99,803 38,675 36,204 38,363 36,058 36,058 37,269 40,000 40,000 36.803 37,047 50,738 38,135 38.139 39.394 41.408 36,118 35,574 23-Feb-95 3-Feb-99 29-Sep-97 06-MAR- 1998 23-Nov-99 6-Jul-98 1 2-Jun-98 12-Jun-98 1-Feb-99 07-OCT- 1999 07-OCT- 1999 20-Nov-99 17-Jun-98 7-Jun-93 29-Nov-99 17-Jun-98 4-Aug-99 28-Aug-98 23-OEC- 1998 23-DEC- 1998"*'- 30-Nov-95 rxa01253 873 GByPL2:F508 GB-PI-2:508 GBINI:CELC06G1 31205 U41014 Caenorhabditis elegans, cosmid CO6G1.

Caenorhabditis elegans 38,560 2006200807 24 Feb 2006 rxa01321 1044 GBGSSI4:AQ51884 441 3 GB-HTG2:AC007473 194859 AQ51 8843 AC007473 Table 4 (continued) HS -5106_-Al_-D10OSP6E RPCI-11 Human Male BAC Library Homo sapiens Homo sapiens, genomic clone Plate=682 Col=19 Row--G, genomic survey sequence.

Drosophila melanogasler chromosome 2 clone BACR38D12 (13590) RPCI-98 D rosophila mela nog aster 38.D.12 map 48A-48B strain y; cn bw sp, -SEQUENCING IN PROGRESS 60 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR35FOl (Di 156) RPCI-98 Drosophila melanogaster 35.17.1 map 48A-48C strain y; on bw sp, SEQUENCING IN PROGRESS 108 unordered pieces.

GB-HTG4:AC011696 115847 AC011696 rxa01352 705 GBPL2:ATACOO5l67 83260 GB-PL2:ATAC005825 97380 GB-HTG3:AC01 1150 127222 rxaOI360 259 rxa0l36l 629 GBEST32:A1725583 GB-PR2:HS227P1 7 GB-EST34:AV171099 GB-RO:AB008g1 5S1 GBE5T22:A1050532 GB. RO:AB008895 rxa01381 944 GBPLl:AB005237 GBGSS5:AQ766840 728 82951 173 530 293 3062 87835 491 68848 2374 40337 112184 2374 AC005167 Arabidopsis thaliana chromosome 11 BAC F1 2A24-genomic sequence, complete sequence.

AC005825 Arabidopsis thaliana chromosome 11 BAC T24121 genomic sequence, complete sequence.

AC01 1150 Homo sapiens clone 4-K-17, LOW-PASS SEQUENCE SAMPLING.

A1725583 BNLGHi12371 Six-day Cotton fiber Gossypium hirsutum cDNA 5 similar to (U86081) root hair defective 3 [Arabidopsis thaliana], mRNA sequence.

Z81007 Human DNA sequence from PAC 227P17, between markers DXS6791 andDXS8O38 on chromosome X contains CpG island, EST.

AV171099 AV1 71099 Mus musculus head C578L16J 14, 17 day embryo Mus musculus cOMA clone 3200002M1 1, mRNA sequence.

AB008915 Mus musculus mGpil gene. exon 1.

A1050532 uc83dl O.yl Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE:1432243 5 similar to TR:035120 035120 MGPI1P. mRNA sequence.

AB008895 Mus musculus mRNA for mGpilp, complete cds.

AB005237 Arabidopsis thaliana genomic DNA, chromosome 5, PI clone: MJJ3,complete sequence.

AQ766840 HS_2026_A2_C09_T7C CIT Approved Human Genomic Sperm Library 0 Homo sapiens genomic clone Plate=2026 Col=18 Row--E, genomic survey sequence.

AL022004 Mycobacterium tuberculosis H37Rv complete genome: segment 40/1 62.

X96471 C.glutamicum lysE and lysG genes.

AL031 107 Streptomyces coelicolor cosmid 5A7.

AC004054 Homo sapiens chromosome 4 clone 8220G8 map 4q21, complete sequence.

X96471 C.glutamicum lysE and IysG genes.

AQ769223 HS-3155-.82-.GI10.j7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3155 Col=20 Row--N, genomic survey sequence.

Arabidopsis thaliana Arabidlopsis thaliana Homo sapiens Gossypiumn hirsutumn Homo sapiens Mus musculus Mus musculus Mus musculus Mus musculus Arabidopsis thaliana Homo sapiens Mycobacteriumn tuberculosis Corynebacterium glutamicum Streptomyces coelicolor Homo sapiens Corynebacterium glutamicumn Homo sapiens 41,121 40,634 38,290 34,311 34,311 37,722 38,492 39,738 46,237 45.574 44,097 41,316 36,606 37.916 37,419 34,831 35.1 38 37.277 100,000 38,400 1999 2-Aug-99 26-OCT- 1999 1 1998 12-Apr-99 0l-OCT- 1999 1 1-Jun-99 23-Nov-99 6-Jul-99 28-Sep-99 9-Jul-98 23-Nov-97 20-Nov-99 28-Jul-99 24-Jun-99 24-Feb-97 27-Jul-98 9-Jul-98 24-Feb-97 28-Jul-99 rxa01393 993 rxa01394 822 GBBA1:M1V043 GB GAl :CGLYSEG GB BA1 :C5A7 GBPR3:AC004054 GB-BA1 :CGLYSEG GBG555:AQ769223 500 2006200807 24 Feb 2006 GB-BA1:CGLYSEG 2374 X96471 rxa01416 630 rxa01442 1347 rxa01446 1413 rxa01483 1395 rxa01486 757 rxa01489 1146 rxaOl49l 774 1662 rxa01512 723 GBBA1:SC3C3 31382 GBBA1 :MLCB22 40281 GBBA1:MTVO02 56414 GBBA1:D90827 18886 GB-BA1 :D90828 14590 GBBA2:AE000279 10855 GBBA1:SC~I-I 39524 GBB3AI:MTVI3E1O 35019 GBBA1:MLCB4 36310 GB-BA1 :MTCY98 31225 GBBA1:MSGB1229C 30670

S

GB_8A2:AF027507 5168 GBBA1:MTVOO2 56414 GB BA1 :MLCB22 40281 GBBA1:SC3C3 31382 GBBAl CO RFADS 1.547 GB BA1 :MLCB22 40281 GBBA1:SC1OA7 39739 GBBA1:MTV0O2 56414 GBEST13:AA356956 255 GB-OV:OMDNAPROI 7327 GB IN1:CEF28C12 14653 GBI[N1:CEF28C12 14653 GB-BA1:SCE9 37730 GB-BA1:MAU88875 840 AL031 231 Z98741 AL008967 D90827 D90828 AE000279 AL049754 Z95324 AL023514 Z83860 1-788 12 AF027507 AL008967 Z98741 AL031231 D37967 Z98741 AL078618 AL008967 AA356956 X92380 Z93380 Z93380 AL049841 U88875 Table 4 (continued) C.glutamicum iysE and lysG genes.

Streptomyces coelicolor cosmid 3C3.

Mycobacterium leprae cosmid B22.

Mycobacterium tuberculosis H37Rv complete genome; segment 122/1 62.

E.coli genomic DNA, Kohara clone #336(41.2-41.6 min.).

Ecoli genomic DNA, Kohara clone #336gap(41 .6-41.9 min.).

Escherichia coil K-12 MG1655 section 169 of 400 of the complete genome.

Streptomyces coelicolor cosmid H10.

Mycobacterium tuberculosis H37Rv complete genome; segment 18/162.

Mycobacterium leprae cosmid 134.

Mycobacterium tuberculosis H37Rv complete genome: segment 103/1 62.

Mycobacterium leprae cosmid B1229 DNA sequence.

Mycobacterium smegmatis dGTPase (dgt), and primase (dnaG) genes, complete cds; tRNA-Asn gene, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 1221162.

Mycobacterium Ieprae cosmid 822.

Streptomyces coelicolor cosmid 3C3.

Corynebacterium ammoniagenes gene for FAD synthetase. complete cdls.

Mycobacterium Ieprae cosmid 822.

Streptomyces coelicolor cosmid 1OAT.

Mycobacterium tuberculosis H37Rv complete genome: segment 1221162.

EST65614 Jurtkat T-cells III Homo sapiens cDNA 5Vend, mRNA sequence.

O.mossambicus prolactin I gene.

Caenorhabditis elegans cosmid F28C1 2, complete sequence.

Caenorhabditis elegans cosmid F28C12, complete sequence.

Streptomyces coelicolor cosmid E9.

Mycobacterium avium hypoxa nthine-g ua nine phosphoribosyl transterase gene, complete cds.

Corynebacterium 33.665 glutamicum Streptomyces coelicolor 62.726 Mycobacterium leprae 39,159 Mycobacterium 37,340 tuberculosis Escherichla coi 58,517 Escherichia coi 56.151 Escherichia col 56,021 Streptomyces coelicolor 39,037 Mycobacterium 40,130 tuberculosis Mycobacterium leprae 37,752 Mycobacteriumn 39,057 tuberculosis Mycobacterium leprae 54,382 Mycobacterium smegmatis 52,941 Mycobacterium 40,941 tuberculosis Mycobacterium leprae 38,451 Streptomyces coelicolor 61,194 Corynebacterium 58,021 ammoniagenes Mycobacterium leprae 38.414 Streptomyces coelicolor 36,930 Mycobacterium 37,062 tuberculosis Homo sapiens 37,647 Tilapia mossambica 38,289 Caenorhabditis elegans 37,984 Caenoriabditis elegans 38,469 Streptomyces coelicolor 39,021 Mycobacterium avium 57,521 24-Feb-97 10O-Aug-98 22-Aug-97 17-Jun-98 21-MAR- 1997 21-MAR- 1997 12-Nov-98 04-MAY- 1999 17-Jun-98 27-Aug-99 17-Jun-98 15-Jun-96 1 6-Jan-98 17-Jun-98 w..

22-Aug-97 10O-Aug-98 8-Feb-99 22-Aug-97 9-Jun-99 17-Jun-98 21 -Apr-97 19-OCT- 1995 23-Nov-98 23-Nov-98 1 9-MAY- 1999 os-MAR- 1997 2006200807 24 Feb 2006 GB_BA1:M1Y15C1O 33050 rxa01514 711 GBBA1:MTCY7H7B 24244 GB,,BA1 :MLCB2548 38916 GB-PL1:EGGTPCHI 242 rxa0l 515 975 GBBA1:ECOUW93 338534 GBBA1:ECOUW93 338534 GB-BA1 :MTCY49 39430 rxa0l5l6 513 GBIN1:DME238847 5419 GBHTG3:AC009210 103814 GB_1N2:AF132179 4842 rxa0l5l7 600 GBPL2:F6H8 82596 GBPL2:AF038831 647 GBPL2:ATACOOS957 108355 rxa0l 521 921 GB-BA1 :ANANIFBH 5936 GBPR2:AC002461 197273 GBPR2:AC002461 197273 rxa0lS28 651 GBRO:MM437P9 165901 GBPR3:AC005740 186780 GBPR3:AC005740 186760 =01~5511 1998 GBBA1:MTCY22G1O 35420 GBBA2:ECOUW89 176195 GBBA1:SCQ1 1 15441 rxaOl56l 1053 GB-IN1 :CEY62H9A 47396 GB-PR4:HSU51003 3202 GS OM:PIGDAO1 395 rxa01599 1785 GB-BA1:MTCI125 37432 GB _BA1I:U000211 39193 Z95436 Z95557 AL023093 Z49757 U14003 U14003 Z73966 AJ238847 AC00921 0 AF132179 AF1 78045 AF038831 AGO05957 J051i11 AC002461 AC002461 AL049866 AC005740 AC005740 Z84724 U00006 AL096823 AL032630 U51 003 M18444 Z98268 U00021 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 154/162. Mycobacterium tuberculosis Mycobacteriurm tuberculosis H37Rv complete genome; segment 153/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B2548. Mycobacterium leprae E.gracilis mRNA for GTP cyclohydrolase I (core region). Euglena gracilis Eschenichia coli K-12 chromosomal region from 92.8 to 00.1 minutes. Escherichia coli Escherichia coli K-1 2 chromosomal region from 92.8 to 00. 1 minutes. Escherichia coli Mycobacterium tuberculosis H37Rv complete genome; -segment 93/162. Mycobacterium tuberculosis Drosophila melanogaster mRNA for drosophila dodeca-satellite protein 1 Drosophila melanogaster (DDP-1).

Drosophila melanogaster chromosome 2 clone BACR01 106 (01054) RPCI-98 Drosophila melanogaster 01.1.6 map 55D-55D strain y: cn bw sp, SEQUENCING IN PROGRESS unordered pieces.

Drosophila melanogaster clone LD21677 unknown mRNA. Drosophila melanogaster Arabidlopsis tlhaliana BAC F6H8. Arabidlopsis thaliana Sorosporium saponariae internal transcribed spacer 1, 5.8S ribosonmal RNA Sorosporium saponariae gene; and internal transcribed spacer 2, complete sequence.

Arabidlopsis thaliana chromosome 11 BAC T15J14 genomic sequence. Arabidlopsis thaliana complete sequence.

Anabaena sp. (clone AnH2O.1) nitrogen fixation operon nifB, fdxN, nifS, nifU, Anabaena sp.

and nifH genes, complete cdls.

Human BAC clone RG204116 from 7q31, complete sequence. Homo sapiens Human BAC clone RG20411 6 from 7q31, complete sequence. Homo sapiens Mus musculus chromosome X, clone 437P9. Mus rnusculus Homo sapiens chromosome 5p, BAC clone 50g2l (LBNL H1 54), complete Homo sapiens sequence.

Homo sapiens chromosome 5p, SAC clone 50g2l (LBNL H 154), complete Homo sapiens sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 21M162. Mycobacterium tuberculosis E. cohl chromosomal region from 89.2 to 92.8 minutes. Escherichia col Streptomyces coelicolor cosmid 011. Streptomyces coelicolor Caenorhabditis elegans cosmid Y62H9A, complete sequence. Caenorhabditis elegans Homo sapiens DLX-2 (DLX-2) gene, complete cds. Homo sapiens Pig 0-amino acid oxidlase (DAO) gene, exon 1. Sus scrofa Mycobacterium tuberculosis H37Rv complete genome; segment 76/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid L247. Mycobacterium leprae 40,086 43.343 38,177 64,876 38,943 37,500 38,010 36,346 37,897 36,149 35,846 40,566 38,095 38,206 36,623 34,719 37,500 37,031 38,035 38,371 38,064 60,775 38,514 37,730 39,340 63,300 36,756 17-Jun-98 18-Jun-98 27-Aug-99 1995 17-Apr-96 17-Apr-98 24-Jun-99 13-Aug-99 20-Aug-99 3-Jun-99 1 9-Aug-99 13-Apr-99 7-Jan-99 26-Apr-93 20-Aug-97 20-Aug-97 29-Jun-99 Ol-OCT- 1998 Ol-OCT- 1998 17-Jun-98 17-DEC- 1993 8-Jul-99 2-Sep-99 07-DEC- 1999 27-Apr-93 1 7-Jun-98 29-Sep-94 2006200807 24 Feb 2006 Table 4 (continued) Mycobacterium leprae cosmid B31351.

rxaCl617 795 rxa01657 723 rxa1S660 675 rxa01678 651 jxa0l 679 1359 rxa0l 690 1224 rxa01692 873 rxa01698 1353 GB-BA1 :MLCB1 351 GBPR2:HSMTMO GBPR2:HSi3DIO GB BA1:MTCY1A10 GBEST6:D79278 GB-BA2:AF1 29925 GBBA1:MTV013

GBRO:MMFVI

GB-PAT:A67508 GBV11TVU95309 GBVI:1VU95303 GBVI:TVU95302 GBEST5:H91843 GB STS:G26925 GBYPL2:AFI 39451 G13BA1 :SC1 C2 GB-EST22:A1064232 GB IN2:AF117896 GBBA2:AF067123

GB-RO:RATNFHPEP

GB-R0:RSNFH GBBA2:AF124600 38936 Z95117 Mycobacterium leprae 217657 AL034384 Human chromosome Xq28, cosmid clones 71-3, 14D.7, C1 230, I1I E7. F1 096, H-omo sapiens 153147 217657 25949 392 10243 11364 6480 6480 600 600 600 362 362 1202 42210 493 1020 1034 3085 3085 4115 AL021407 AL034384 Z95387 079278 AF1 29925 AL021 309 X97719 A67508 U95309 U95303 U95302 H91843 G26925 AF139451 ALO031124 A1064232 AF1 17896 AF067123 M37227 X1 3804 AF1 24600 A12197. 12G8, A091O0; complete sequence bases 1. .217657.

Homo sapiens DNA sequence from PAC 13D10 on chromosome 6 p22.3-23.

Contains CpG island.

Human chromosome Xq28, cosmid clones 71-3, 14D37, C1230. 1 1E7, F1096, Al12197, 12G8, A091 00; complete sequence bases 1. .217657.

Mycobacterium tuberculosis H37Rv complete genome; segment 117/162.

HUM213006B Human aorta polyA+ (TFujiwara) Homo sapiens cONA clone GEN-213D06 mRNA sequence.

Thiobacillus ferrooxidans carboxysome operon. complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 134/162.

Homo sapiens Homo sapiens Mycobacterium tuberculosis H-omo sapiens Thiobacillus ferrooxidans Mycobacterium tuberculosis 36,756 40,811 38,768 39,018 40.656 44.262 40.709 40,986 35.364 35,364 41,1194 41,712 39,576 39,157 24-Jun-97 5-Jul.99 23-Nov-99 5-Jul-99 17-Jun-98 9-Feb-96 1 7-MAY- 1999 17-Jun-98 29-Aug-96 1999 28-OCT- 1997 28-OCr- 1997 28-OCT- 1997 29-Nov-95 M.musculus retrovirus restriction gene Fyi. Mus musculus Sequence 1 from Patent W0974341 0. Mus musculus Tula virus 064 nucleocapsid protein gene, partial cds. Tula virus Tula virus 052 nucleocapsid protein gene, partial cds. Tula virus Tula virus 024 nucleocapsid protein gene, partial cds. Tula virus ys8le~l.sl Scares retina N2b4HR Homo sapiens cONA clone Homo sapiens IMAGE:221208 3' similar to gb:X63749_rnal GUANINE NUCLEOTIDE- BINDING PROTEIN ALPHA-i (HUMAN);, mRNA sequence.

human STS SHGC-30023, sequence tagged site. Homo sapiens Gossypium robinsonii CeIA2 pseudogene, partial sequence. Gossypium robinsonii Streptomyces coelicolor cosmid 1C2. Streptomyces coelicolor GH04563.5prime GH Drosophila melanogaster head pOT2 Drosophila Drosophila melanogaster melanogaster cONA clone GH04563 Sprime, mRNA sequence.

Drosophila melanogaster neuropeptide F (npl) gene, complete ods. Drosophila melanogaster Lactobacillus reurteri cobalamin biosynthesis protein J (cbiJ) gene, partial cds; Lactobacillus reuteri and uroporphyrin-Ill C-methyltransferase (sumT) gene, complete cds.

Rat heavy neurofilament (NF-H) polypeptide, partial cds. Rattus norvegicus Rat mRNA for heavy neurofilament polypeptide N F-I- C-terminus. Rattus sp.

Corynebacterium g lutamicum chorismate synthase (a roC), shikimate kinase Corynebacteriumn (aroK), and 3-dehydroquinate synthase (aroB) genes, complete ods: and glutamnicumn putative cytoplasmic peptidase (pepO) gene, partial cds.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 1 11/162. Mycobacterium tuberculosis 39.157 14-Jun-96 38,910 1-Jun-99 60,644 15-Jan-99 38,037 24-Nov-98 36,122 2-Jul-99 48,079 3-Jun-98 37.093 27-Apr-93 37,093 14-Jul-95 100,000 04-MAY- 1999..

36,323 17-Jun-98 GBBA1:MTCY1 59 33818 Z83863 2006200807 24 Feb 2006 GB-BA1:MSGB937C 38914

S

rxa01699 693 GBBA2:AF124600 4115 GBBA2:AF016585 41097 GBEST9:C19712 399 rxa01712 805 GB-EST21:AA952466 278 GBEST21:AA952466 278 rxa0l 719 684 nca0l72O 1332 rxa0ll46 876 rxa01747 1167 rxa0l 757 924 GB-HTG1:HSDJ534K 154416 7 GBHTGI:HSDJ534K 154416 7 GBEST27:A1447108 431 GB-PR4:AC006322 179640 GBPL2:TMO18A1O 106184 GBPR4:AC006322 179640 GBEST3:R46227 443 GBEST3:R46227 443 GBBA1:MTCY19O 34150 GBBA1:MLC622 40281 GB-BAI:SC5F7 40024 GBEST21:AA918454 416 GBEST4:H34042 345 GBEST2O:AA899038 450 Table 4 (continued) L78820 Mycobacterium leprae cosmid B937 DNA sequence. Mycobacterium lepr~ AF124600 Corynebacterium glutamicum chorismate synthase (aroC), shikimate kinase Corynebactenumr (aroK), and 3-dlehydroquinate synthase (araB) genes, complete cds; and glutamicum putative cytoplasmic peptidlase (pepO) gene, partial cds.

AF016585 Streptomyces caelestis cytochrome P-450 hydroxylase homolog (nidi) gene, Streptomyces caele partial cds; polyketidle synthase modules 1 through 7 (nidA) genes, complete cds; and N-methyllransferase homolog gene, partial cdls.

C19712 C1 9712 Rice particle at ripening stage Oryza sativa cONA clone E10821lA, Oryza sativa mRNA sequence.

AA952466 TENS14O4 T. cruzi epimastigote normalized cONA Library Trypanosoma cruzi Trypanosoma cruzi cDNA clone 1404 mRNA sequence.

AA952466 TENS1404 T. cruzi epimastigote normalized cONA Library Trypanosoma cruzi Trypanosoma cruzi cDNA clone 1404 mRNA sequence.

AL109925 Homo sapiens chromosome 1 clone RP4-534K7, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

AL109925 Hlomo sapiens chromosome 1 clone RP4-534K7, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

A1447108 mq9leOS.xl Stratagene mouse heart (#937316) Mus musculuscONA dlone Mus musculus IMAGE:586118 mRNA sequence.

AC006322 Homo sapiens PAC clone DJ106OBll from 7q11.23-q21.1. complete Homo sapiens sequence.

AF013294 Arabidlopsis Ihaliana BAC TMO1 8A1O0. Arabidlopsis thaliana AC006322 Homo sapiens PAC clone DJ106OB1 1 from 7q1 1.23-q21.1, complete Homo sapiens sequence.

R46227 yg52a03.sl Soares infant brain iNIB Homo sapiens cDNA clone Homo sapiens IMAGE:36000 mRNA sequence.

R46227 yg52a03.sl Soares infant brain INIB Homo sapiens cONA clone Homo sapiens IMAGE:36000 mRNA sequence.

Z70283 Mycobacterium tuberculosis H37Rv complete genome; segment 981162. Mycobacterium stis 62,780 100,000 40,260 45.425 40,876 41,367 35,651 35,651 39,671 35,817 35,698 37,243 42,812 42,655 59,294 57,584 61.8 10 39,655 35,942 40.000 1 5-Jun-96 04-MAY- 1999 07-DEC- 1997 24-OCT- 1996 29-OCT- 1998 29-OCT- 1998 23-Nov-99 23-Nov-99 09-MAR- 1999 18-MAR- 1999 12-Jul-97 18-MAR- 1999 22-MAY- 1995 22-MAY- 1995 17-Jun-98 22-Aug-97 22-Jul-99 23-Jun-98 2-Aprr98 12-Apr-98i Z98741 AL096872 AA91 8454 H34042 AA899038 tuberculosis Mycobacteriumn leprae cosmid B22. Mycobacterium lepr Streptomyces coelicolor cosmid 5F7. Streptomyces coelic A3(2) om38c02.sl SoaresNFLTGBCSi Homo sapiens cIJNA dlone Homo sapiens IMAGE:1 543298 3'similar to WP:F28F8.3 CE09757 SMALL NUCLEAR RIBONUCLEOPROTEIN E mRNA sequence.

EST1 10563 Rat PC-12 cells, NGF-trealed (9 days) Rattus sp. cDNA clone Rattus sp.

RPNBI81 5' end, mRNA sequence.

NCP6G8T7 Perithecial Neurospora crassa cDNA dlone NP6G8 3'end, mRNA Neurospora crassa sequence.

:olor 2006200807 24 Feb 2006 rxa0l8O7 915 GBBA1:AP000063 GBHTG4:AC010694 GBHTG4:ACO 10694 =xa1821 401 GB_BA1:CGL007732

GB_RO:RATALGL

GB OV:APIGY2 rxa01835 654 GBEST3O:AI 629479 GBSTS:G48245 GBGSS3:B49052 rxa0l85O 1470 GB -BA2:ECOUW67_ 0 GBBA2:AE000392 GBBA2:U32715 rxa01878 1002 GBHTG1:CEY64F11 GBHTG1:CEY64Fl1 GB-HTG1 :CEY64F1 1 rxa01892 852 GB8BA1:MTCY274 GIB BA1 :MLCB250 GB-BA1 :MSGB1 5290

S

rxa01894 978 GB-BA1:MTCY274 GBIN1:CELF46H5 GByHTG3:AC009204 185300 115857 115857 4460 7601 1381 353 515 515 110000 10345 13136 177748 177748 177748 39991 40603 36985 39991 38886 115633 AP000063 AC01 0694 AC01 0694 AJ007732 M24108 X78272 A1629479 G48245 B49052 U18997 AE000392 U32715 Z99776 Z99776 Z99776 Z74024 Z97369 L78824 Z74024 U41543 AC009204 AF112536 Y09572 Table 4 (continued) Aeropyrum pemix genomic DNA. section 6M7 Drosophila melanogaster clone RPCI9B-6H2, SEQUENCING IN PROGRESS 75 unordered pieces.

Drosophila melanogaster clone RPCI98-6H-2, SEQUENCING IN PROGRESS 75 unordered pieces.

Corynebacterium glutamicum 3'ppc gene. secG gene. amt gene, ocd gene and 5' soxA gene.

Rattus norvegicus (clone A2U42) alpha2u globulin gene, exons 1-7.

Anas platyrhynchos (Super M) IgY upsilon heavy chain gene. exon 2.

486101D10.xl 486 leaf primordia cDNA library from I-ake lab Zea mays cDNA, mRNA sequence.

SHGC-62915 Human Homo sapiens STS genomic, sequence tagged site.

RPCI1 114112.TV RPCI-1 1 Homo sapiens genomic clone RPCI-1 14112, genomic survey sequence.

Escherichia col K-1 2 chromosomal region from 67.4 to 76.0 minutes.

Escherichia coli K-12 MG 1655 section 282 of 400 of the complete genome.

Haemophilus influenzae Rd section 30 of 163 of the complete genome.

Caenorhabditis elegans chromosome IV clone Y64F1 1, SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome IV clone Y64171 1, SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome IV clone Y64F1 1, SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 1261162.

Mycobacterium Ieprae cosmid B250.

Mycobacterium leprae cosmid 51529 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 126/1 62.

Aeropyrum pernix Drosophila melanogaster Drosophila melanogaster Corynebacterium glutamicum Rattus norvegicus Anas platyrhynchos Zea mays Homo sapiens Homo sapiens Escherichia coli Escherichia coli Haemophilus influenzae Rd Caenorhabditis elegans Caenorhabditis elegans Caenortiabditis elegans Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae Mycobacterium tuberculosis Caenorhabditis elegans 40,067 35,450 35,450 100,000 38,692 36,962 38,109 37,021 37.021 37.196 38,021 39,860 37,564 37,564 37,576 35.910 64,260 64,260 37,229 38,525 31,579 99,733 70,321 22-Jun-99 1 6-OCT- 1999 16-OCT- 1999 7-Jan-99 1994 15-Feb-99 26-Apr-99 26-MAR- 1999 8-Apr-99 U18997 12-Nov-98 29-MAY- 1998 1 4-OCT- 1998 14-OC- 1998 14-OCT- 1998 19-Jun-98 27-Aug-99 15-Jun-96 19-Jun-98 29-Nov-96 18-Aug-99 5-Aug-99 18-Apr-P8 Caenorhabditis elegans cosmid F46H5.

rxa~i 920 1125 GBBA2:AF1 12536 1798 GBBA1:CANRDFGE 6054

N

Drosophila melanogaster chromosome 2 clone BACRO3E19 (D1033) RPCI-98 Drosophila melanogaster 03.E.19 map 36E-37C strain y; cn bw sp, SEQUENCING IN PROGRESS 94 unordered pieces.

Corynebacterium glutamicum ribonucteotide reductase beta-chain (nrdF) Corynebacterium gene, complete cds. glutamicum Corynebacterium ammoniagenes nrdH, nroll, nrdE, nrdF genes. Corynebacterium ammoniagenes 2006200807 24 Feb 2006 Table 4 (continued) 1228 AF050168 Corynebacterium ammoniagenes ribonucleoside diptiosphate reductase small Corynebacterium GBBA2:AF050168 rxa01 928 960 rxa01929 936 rxa0I 94D 1059 rxa02022 1230 rxa02O24 859 GBBAl :CGPAN GB-PL1 :AP000423 GBPL1:AP000423 GBBA1:CGPAN GB-BAI:XCU33548 GBBA1:XANHRPB6

A

GB_1N2:CFU43371 GB-BA2:AE001467 GB-RO:AF1 75967 GBBA1:CGDAPE GBBA1:CGDNAARO

P

GBBAl :APU47055 GBBA1:MTC1364 2164 154478 154478 2164 8429 1329 1060 11601 3492 1966 2612 6469 29540 X96580 AP000423 AP000423 X96580 U33548 M99174 U43371 AE001 467 AF175967 X81379 X85965 U47055 Z93777 L01536 U1 51180 subunit (nrdF) gene, complete cds.

Oglutamicum panB. panO xylB genes.

Arabidopsis thaliana chioroplast genomic DNA, complete sequence, strain:Columbia.

Arabldopsis thaliana chioroplast genomic DNA, complete sequence, strain:Columbia.

C.glutamicum panB, panC xylB genes.

Xanthomonas campestris hrpB pathogenicity locus proteins HrpBl, HrpB2, HrpB3, HrpB4, HrpB5, HrpB6, HrpB7, HrpB8, HrpAl, and QRF62 genes, complete cds.

Xanthomonas campestris hrpB6 gene, complete ods.

Crithidia fasciculala inosine-uridine preferring nucleoside hydrolase (IUNH) gene, complete cds.

Helicobacter pylori, strain J99 section 28 of 132 of the complete genome.

Homo sapiens Leman coiled-coil protein (LCCP) mRNA, complete cds.

COglutamicum dapE gene and orf2.

Oglutamicum ORF3 and aroP gene.

Anabaena PCC7120 nitrogen fixation proteins (nifE, nufN, nifX, MiW genes, complete cds, and nitrogenase (nifK) and hesA genes, partial cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 52/162.

M. leprae genomic dna sequence, cosmid b1912.

Mycobacterium leprae cosmid B1756.

ammoniagenes Corynebacterium glutamicum Ohlo roplast Arabidopsis thaliana Chloroplast Arabidopsis thaliana Corynebacterium glutamicum Xanthomonas campestris pv. vesicatoria Xanthomonas campestris Crithidia fasciculata Helicobactr pylorn J99 Mus musculus Corynebacterium glutamicum Corynebacterium glutamicum Anabaena PCC7120 Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae 72,082 100,000 35,917 33,925 100,000 38,749 39,305 61,417 38,560 40,275 100,000 38,889 36,647 59,415 57,093 57,210 23-Apr-98 11 -MAY- 1999 15-Sep-99 11 -MAY- 1999 19-Sep-96 1 4-Sep-93 1 8-Jun-96 20-Jan-99 26-Sep-99 8-Aug-95 30-Nov-97 1 7-Feb-96 17-Jun-98 1 4-Jun-96 09-MAR- 1995 GB BA1 :MSGB1 91 20 38503

S

GB-BA1:MLU1518O 38675 rxa02027 rxa02031 rxa02072 1464 GBBAl :CGGDHA 2037 GB-BAI:CGGDH 2037 GB-BA1:PAE18494 1628 X72855 X59404 Y18494 COglutamicumn GDH-A gene.

Corynebacterium glutamicum, gdh gen for glutamate dehydrogenase.

Pseudomonas aeruginosa gdhA gene. strain PACI.

Corynebacterium glutamicum Corynebacterium glutamicum Pseudomonas aeruginosa 99,317 94,387 62.247 24-MAY- 1993 30-Jul-99 6-Feb-99 2006200807 24 Feb 2006 rxa02O85 2358 rxa02O93 927 rxaO2lO6 1179 rxaO2ll11 1407 rxaO2ll2 960 rxa02134 1044 rxa02135 1197 GBBA1 :MTCY22G8 22550 GB_ BA1:MLCB33 42224 GB-BA1:ECOUW85 91414 GB-EST14:AA448146 452 GBEST17:AA641937 444 GB PR3:AC003074 Gb BAII:SCIA6 GBPR4:AC005553 GB-EST3:R49746 GB-BA1 :SC6G1 0 GB-BA1 :U0001 0 GBBA1:MTCY336 GBHTG3:AC010579 GBGSS3:B09839 GB-HTG3:AC01 0579 143029 37620 179651 397 36734 41171 32437 157658 1191 157658 Z95585 Z94723 M87049 AA4481 46 AA641937 AC003074 AL023496 AC005553 R49746 AL049497 U00010 Z95586 AC01 0579 B09839 AC01 0579 X83011 A1731 596 X8301 1 AIL0231507 AL022347 U89959 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 49/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B33. Mycobacterium Ieprae E. coi genomic sequence of the region from 84.5 to 86.5 minutes. Escherichia coi zw82h01.rl Soares-testis NHT Homo sapiens cDNA dlone IMAGE:782737 Homo sapiens mRNA sequence.

nsl8blO.rl NCI CGAPGCB1 Homo sapiens cONA clone IMVAGE:1 183963 Homo sapiens mRNA sequence.

Human PAC clone DJ0596009 from 7p15, complete sequence. Homo sapiens Streptomyces coelicolor cosmid 1A6. Streptomyces coelicolor Homo sapiens chromosome 17, clone hRPK.1 12J9, complete sequence. Homo sapiens yg7lglO .0l Soares infant brain 1iNIB Homo sapiens cDNA cfone Homo sapiens IMAGE:38768 5' similar to gb:V00567 BETA-2-MICROGILOBULIN PRECURSOR (HUMAN);, mRNA sequence.

Streptomyces coelicolor cosmid 6G1 0. Streptomyces coelicolor Mycobacterium leprae cosmid B1 170. Mycobacterium leprae Mycobacterium tuberculosis H37Rv complete genome; segment 70/162. Mycobacterium tuberculosis Drosophila melanogaster chromosome 3 clone BACRO9008 (D1 101) RPCI-98 Drosophila melanogaster 09.D.8 map 96F-96F strain y; on bw sp, -SEQUENCING IN PROGRESS 121 unordered pieces.

T12A1 2-Sp6 TAMU Arabidopsis Ihaliana genomic clone T1 2A1 2, genomic Arabidopsis thaliana survey sequence.

Drosophila melanogaster chromosome 3 clone BACRO910S (01101) RPCI-98 Drosophila melanogaster 09.D.8 map 96F-96F strain y; on bw sp, SEQUENCING IN PROGRESS **,121 unordered pieces.

S.coelicolor secY locus DNA. Streptomyces coelicolor BNLGHiI 01 85 Six-day Cotton fiber Gossypium hirsutum cDNA 5' similar to Gossypium hirsutum (AC004005) putative ribosomal protein L7 [Arabidopsis thaliana], mRNA sequence.

Scoelicolor secY locus DNA. Streptomyces coelicolor H-uman DNA sequence from clone RP3-525L-6 on chromosome 6p22.3-23 Homo sapiens Contains CA repeat, STSs, GSSs and a CpG Island, complete sequence.

Arabidopsis thaliana DNA chromosome 4, BAC clone F21P8 (ESSA project). Arabidopsis thaliana Arabidopsis Ihaliana 8AC T7123, complete sequence. Arabidopsis thaliana 38.442 56,486 52,127 34,163 35.586 31,917 35,818 34,274 41,162 50,791 37,563 39,504 37,909 37,843 37,909 36,533 33,451 36,756 34.365 34,325 33.874 17-Jun-98 24-Jun-97 29-MAY- 1995 4-Jun-97 27-OCT- 1997 6-Nov-97 13-Jan-99 31-DEC- 1998 18-MAY- 1995 24-MAR- 1999 01-MAR- 1994 24-Jun-99 24-Sep-99 14-MAY- 1997 24-Sep-99 02-MAR- 1998 11-Jun-99 02-MAR- 1998 23-Nov-99 9-Jun-99 26-Jui-98 GB-BAl :SCSECYDN 6154

A

GB-EST32:AI731 596 568 GB-BA1 :SCSECYDN

A

GB-PR3:HS525L6 GBYPL2:ATF21 P8 GB-PL2:U89959 6154 168111 85785 106973 2006200807 24 Feb 2006 rxa02136 645 GBPL2:ATAC005819 57752 AC005819 GB,,PL2:Fl5K9 GB PL2:U89959 rxa02139 1962 GB-BA1:MTCY190 71097 AC005278 106973 U89959 34150 Z70283 Table 4 (continued) Arabidopsis thaliana chromosome 11 BAC T3A4 genomic sequence, complete sequence.

Arabidopsis thaliana chromosome 1 BAC F15K9 sequence, complete sequence.

Arabidopsis thaliana BAC T7123, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

GBBAI:MSGB1554C 36548

S

GB-BAI:MSGB1 551 C 36548

S

GBBA2:AF049897 9196 rxa02153 903 GB-BA1:AF005242 1044 GBBA1:CGARGCJB 4355

D

GBBA2:AF049897 9196 rxa02I54 414 L78814 Mycobacteriumn Ieprae cosmid 131554 DNA sequence.

L78813 Mycobacterium leprae cosmid 81551 DNA sequence.

AF049897 Corynebactenumr glutamicumn N-acetylglutamylphosphate reductase (argC), omnithine acetyltra nsfe rase (argJ), N-aretylglutamate kinase (argB), acetylomithine transaminase (argD). ornithine carbamoyltra nsfe rase (argF). arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

AF005242 Corynebactenium glutamicumn N-acetylglutamate-5-semialdehyde dehydrogenase (argC) gene, complete cds.

X86157 C.glutamicurn argO, argJ, argB, argD. and argP genes.

AF049897 Corynebacterium glutamicum N-acetylglutamnylphosphate reductase (argC), ornithine acetyltransferase (argJ), N-acetylglutamate kinase (argB), acetylomithine transaminase (argD), ornithine carbamoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

AF005242 Corynebactenumr glutamicum N-acetylglutamate-5-semiatdehyde dehydrogenase (argC) gene, complete cds.

X86157 O.glutamicum argC. argJ, argB, argD, and argF genes.

X86157 C.glutamicum argC, argJ. argB, argD, and argF genes.

AF049897 Corynebacterium glutamicum N-acetylg lutamylphos ph ate reductase (argo), ornithine acetyltransferase (argJl), N-acetylglutamate kinase (argB), acetylornithine transaminase (argl)), ornithine carbamoyltransferase (argF), arginine repressor (argR), argininosuccinate syntliase (argG), and argininosuccinate lyase (argH) genes, complete cds.

L7881 1 Mycobactedriu leprae cosmid 81 133 DNA sequence.

AF049897 Corynebacterium glutamicum N-acetylglutamylphosphate red uctase (argC), ornithine acetyltransferase (argJ), N-acetylglutamate kinase (argB), acetylornithine transaminase (argD). omnithine ca rbamoyltra nsfe rase (argF).

arginine repressor (argR), argininosuccinate synthase (argG). and argininosuccinate lyase (argH-) genes, complete cds.

Arabidopsis thaliana Arabidopsis thaliana Arabidopsis thaliana Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebactenium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium Ieprae Corynebacterium glutamicum 34,123 3-Nov-98 31,260 7-Nov-98 34,281 26-Jun-98 62,904 17-Jun-98 36,648 15-Jun-96 36,648 15-Jun-96 99,104 1-Jul-98 99,224 2-Jul-97 100 .000 25-Jul-96 98,551 1-Jul-98 98,477 2-Jul-97 100,000 25-Jul-96 99,767 25-Jul-96 99.378 1-Jul-98 55,504 15-Jun-96 100,000) 1-Jul-98 rxa02155 1287 rxa02156 1074 GBBA1:AF005242 1044 GB-BA1 :CGARGCJB 4355

D

GB-BA1 :CGARGCJB 4355 0 GB-BA2:AF049897 9196 GB-BA1:MSGB1 1330 42106

S

G8-BA2:AF049897 9196 2006200807 24 Feb 2006 GBBA1:CGARGCJB 4355

D

GBBA2:AE001816 10007 GB-BA2:AF049897 9196 rxa02157 1296 fxa02158 1080 GBBAl :CGARGCJB 4355

D

GBBA1:MTCYO6H11 38000 GB8BA2:AF049897 9196 GB_8A2:AF031518 2045 GB_8A1:CGARGCJB 4355

D

Table 4 (continued) X86157 COglutamicum argO. argJ, argB. argD, and argF genes.

AE001816 Thermotoga maritima section 128 of 136 of the complete genome.

AF049897 Corynebacterium glutamicum N-acetylglutamylphosphate reductase (argC), omnithine acetyltransferase (argJ), N-acetylglutamate kinase (argB).

acetylornithine transaminase (argD), ornithine carbamoyltransfe rase (argF).

arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

X86157 COglutamicum argC. argJ, argB, argD, and argF genes.

Z85982 Mycobacteriumn tuberculosis H37Rv complete genome: segment 73/1 62.

AF049897 Corynebacterium glutamicum N-acetylglutamylpliosphate reductase (argC).

ornithine acetyltransferase (argJ), N-acetylglutamate kinase (argB), acetylomithine transaminase (argO), omithine carbamoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

AF031518 Corynebacteriumn glutamicum ornithine carbamolytransferase (argF) gene, complete cds.

X86157 COglutamicum argC. argi. argB, argD. and argF genes.

Corynebactenium glutamicumn Thermotoga maritima Corynebactenumr glutamicumn Corynebacterium glutamicumn Mycobacteriumn tuberculosis Corynebacterium glutamicum Corynebacterium glutamicumn Corynebacteriumn glutamicumn Corynebacteriumn glutamicurn Corynebacteriumn glutamicumn Corynebacteriumn glutamnicumn Corynebacteriumn glutamicumn Corynebacteriumn glutamicum Streptomyces clavuligerus Corynebactenumr glutamicumn 100.000 25-Jul-96 50.238 2-Jun-99 99.612 1-Jul-98 99,612 25-Jul-96 57,278 17-Jun-98 100,000 1-Jul-98 99.898 5-Jan-99 100,000 99,843 25-Jul-96 1-Jul-98 rxa02159 636 GB_8A2:AF049897 rxa0216O 1326 GB_8A2:AF031518 GB-BA2:AF041436 GBBA2:AF049897 GB-BA2:AF030520 GB-BA1 :SCARGGH- GB-BA2:AF049897 9196 AF049897 Corynebacteriumn glutamicumn N-acetylglutamylphosphate reductase (argC).

ornithine acetyltransferase (argJ), N-acetylglutamate kinase (argB), acetylornithine transaminase (argD), omnithine carbamoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

2045 AF031518 Corynebacterium glutamicum omnithine carbamolytransferase (argF) gene, complete cds.

516 AF041436 Corynebacterium glutamicumn arginine repressor (argR) gene, complete cds.

9195 AF049897 Corynebacterium glutamicumn N-acetylglutamylpliosphate red uctase (argO), ornithine acetyltransfe rase (argJl), N-acetylglutamnate kinase (argB), acetylomithine transaminase (argl)), omithine carbamoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

1206 AF030520 Corynebacterium glutamicumn argininosuccinate synthetase (argG) gene.

complete cds.

1909 Z491 11 S.clavuligerus argG gene and argH gene (partial).

9196 AF049897 Corynebactenium glutamicumn N-acetylglutamylphosphate reductase (argC), omithine a cetyltransfe rase (argJ), N-acetylglutamate kinase (argB), acetylornithine transaminase (argD), ornithine carbamnoyltransfe rase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

88,679 5-Jan-99 100,000 5-Jan-99 99,774 99,834 1-Jul-98 1 9-Nov-97 65,913 22-Apr-96 88,524 1-Jul-98 rxa02I62 1554 2006200807 24 Feb 2006 GB-BA2:AF048764 GBBA1:MTCYO6H11 GBBA1:MTCY31 1437 38000 37630 rxa02176 1251 GBBA1:CGGLTG 3013 GB-PL2:PGU65399 2700 rxa02189 861 GBPR3:AC002468 115888 GB BA1 :MSGB1 970C 39399

S

GBPR3AC002468 1 15888 rxa02193 1701 GB-BAI:BRLASPA 1987 GBPAT:E04307 1581 GBBA1:ECOUW93 338534 rxa02194 966 GBBA2:AF050166 840 GB-BA1 :BRLASPA 1987 GB-PATE08649 188 rxa02195 393 GB-BA2:AF086704 264 GBBA1:EAY17145 6019 GB-STS:G01 195 332 rxa02197 551 GBBA1:MTCY261 27322 GB-BA1:MLCB2533 40245 GB-BAI:U00017 42157 rxa02198 2599 GB-BA1:U00017 42157 GBBA1:MLCB2533 40245 GB-BA1:MTCY261 27322 rxa02208 1025 GBBA1:U00017 42157 AF048764 Z85982 Z731 01 X66112 U65399 AC002468 L78815 AC002468 025316 E04307 U14003 AF0501 66 D25316 E08649 AF086704 Y17145 G01195 Z97559 AL03531 0 U00017 U00017 AL03531 0 Z97559 U00017 Table 4 (continued) Corynebacterium glutamicum argininosuccinate lyase (argH) genecomplete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 73/162.

Mycobacterium tuberculosis H37Rv complete genome; segment 411162.

C.glutamicumn git gene for citrate synthase and ORF.

Basidiomnycete CECT 20197 phenoloxidase (pox I) gene, complete cds.

Human Chromosome 1 5q26.1 PAC clone pDJ41 7d7, complete sequence.

Mycobacterium leprae cosmid B 1970 DNA sequence.

Human Chromosome 15q26.1 PAC clone pDJ417d7. complete sequence.

Brevibacterium flavumn aspA gene for aspartase, complete cds.

DNA encoding Brevibacterium flavum aspartase.

Escherichia coli K-12 chromosomal region from 92.8 to 00.1 minutes.

Corynebacterium glutamicumATP phosphonibosyitra nsfe rase (hisG) gene.

complete cds.

Brevibactenium flavumn aspA gene for aspartase. complete cds.

DNA encoding part of aspartase from coryneform bacteria.

Corynebactenium glutamicumn phosphoribosyl-ATP-pyrophospliohydrolase (hisE) gene, complete cds.

Eubacterium acidaminophilum grdR, grdl. grdH genes and partial ldc. grdT genes.

fruit fly STS Dm1 930 clone DS06959 17.

Mycobacterium tuberculosis H37Rv complete genome; segment 95/162.

Mycobacteriumn Ieprae cosmid B2533.

Mycobacterium Ieprae cos mid B21 26.

Mycobacteriumn leprae cosmid B21 26.

Mycobacteriumn leprae cosmid 82533.

Mycobacterium tuberculosis H37Rv complete genome; segment 951162.

Mycobacterium leprae cosniid B2126.

Corynebacterium glutamicum Mycobacteriumn tuberculosis Mycobacterium tuberculosis Corynebacteriumn glutamicumn basidiomnycete CECT 20197 Homo sapiens Mycobacterium leprae Homo sapiens Corynebacterium glutamicumn Corynebacterium glutamicumr Escherichia coli Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebactenium glutamicum Eubacterium acidaminophilum Drosophila melanogaster Mycobacteriumn tuberculosis Mycobacteriumn leprae Mycobacteriumn leprae Mycobacteriumn leprae Mycobacterium leprae Mycobactenumr tuberculosis Mycobacterium leprae 87,561 6.4,732 36,998 39,910 38,474 35,941 40,286 33,689 99,353 99,367 37,651 98.214 93,805 100,000 100.000 39,075 35,542 33,938 65,517 36.770 38,674 65.465 37,577 59,823 1-Jul-98 17-Jun.98 17-Jun-98 17-Feb-95 19-Jul-97 16-Sep-98 1 5-Jun-96 16-Sep-98 6-Feb-99 29-Sep-97 17-Apr-96 5-Jan-99 6-Feb-99 29-Sep-97 8-Feb-99 5-Aug-98 28-Feb-95 17-Jun-98 27-Aug-99 01-MAR- 1994 01 -MAR- 1994 27-Aug-99 17-Jun-98 01-MA:R- 1994' GB.BA1 :AP000063 185300 AP000053 Aeropyrum pernix genomic DNA, section 617.Aeoyupenx942 2-J-9 Aeropyrurn pernix 39,442 22-Jun-99 2006200807 24 Feb 2006 rxa02229 948 rxa02234 3462 nxa02235 727 rxa02237 893 rxa02239 1389 rxa02240 1344 rxa02246 1107 GBPR4:AC006236 127593 GBBA1:MSGY154 40221 GBBA1:MTCY154 13935 GB-BA1:U00019 36033 GBBA1:MSGB937C 38914

S

GBBA1:MTCY2B12 20431 GBBA2:U01072 4393 GB_8A1:MSU91572 960 GBHTG3:AC009364 192791 GBHTG3:AC009364 192791 GBBAI:MTCY21B4 39150 GBBA2:AF077324 5228 GBEST22:AU017763 586 GBBA1:MTCY2iB4 39150 GB-HTG3:AC010745 193862 GBHTG3:AC010745 193862 EM-PAT:E09855 1239 GB-PATA37831 5392 GBBA2:AF1 17274 2303 EMBAl :AB003693 5589 AC006236 AD000002 Z98209 U00019 L78820 Z81011 U01072 U91572 AC009364 AC009364 Z80108 AF077324 AU01 7763 Z80108 AC01 0745 AC01 0745 E09855 A37831 AF117274 AB003693 Table 4 (continued) Homo sapiens chromosome 17, clone hCIT.162_12, complete sequence.

Mycobacterium tuberculosis sequence from clone y154.

Mycobacterium tuberculosis H37Rv complete genome; segment 121/162.

Mycobacterium leprae cosmid B2235.

Mycobacterium leprae cosmid 8937 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 61/162.

Mycobacterium bovis BCG orotidine-5.-monophosphate decarboxylase (uraA) gene.

Mycobacteriumi smegmatis carbamoyl phosphate synthetase (pyrAB) gene, partial cds and orotidine 5'-monophosphate decarboxylase (pyrF) gene, complete cds.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS ',57 unordered pieces.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS 57 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 62/162.

Rhodococcus equi strain 103 plasmid RE-VP1 fragment f.

AU017763 Mouse two-cell stage embryo cONA Mus musculus cDNA clone J0744A04 mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 62/162.

Homo sapiens clone NHO549D1 8. SEQUENCING IN PROGRESS ~,30 unordered pieces.

Homo sapiens clone NHO549D018. -SEQUENCING IN PROGRESS ~.30 unordered pieces.

gDNA encoding S-adenosylniethionine synthetase.

Homo sapiens Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacteriumn leprae Mycobacterium leprae Mycobacterium tuberculosis Mycobacteriumn bovis Mycobacterium smegmatis Homo sapiens Homo sapiens Mycobacterium tuberculosis Rhodococcus equi Mus musculus Mycobacterium tuberculosis Homo sapiens Homo sapiens Corynebacterium glutamicum 37,191 53,541 40,407 40,5411 66,027 71,723 67,101 60,870 37,994 37.994 55,844 41,185 38,616 56,282 36.772 36,772 99,515 63,568 65.000 52,909 29-DEC- 1998 03-DEC- 1996 17-Jun-98 01-MAR- 1994 15-Jun-96 18-Jun-98 22-DEC- 1993 22-MAR- 1997 1-Sep-99 1 -Sep-99 23-Jun-98 5-Nov-98 19-OCT.

1998 23-Jun-98 21-Sep-99 21-Sep-99 07-OCT- 1997 (Rel.

52, Created) 1997 31-MAR- 1999 03-OCT- 1997 (1Rel*.

52, Creited) Sequence 1 from Patent W09408014. Streptomyces pristinaespiralis Streptomyces spectabilis flavoprotein homolog Dfp (dfp) gene, partial cds; and Streptomyces spectabilis S-adenosylmethionlne synthetase (metK) gene, complete cds.

Corynebacterium ammoniagenes DNA for rib operon, complete cds. Corynebacteriumn ammoniagenes 2006200807 24 Feb 2006 GB-PAT:E07957 rxa02247 756 fxa02248 1389 rxa02249 *600 rxa02250 643 rxa02262 1269 rxa02263 488 *GBPAT:132742 GBPAT:132743 EMBA1:AB003693 GBPAT:132742 GB-PAT:132742 EM-BA1:AB003693 GBPAT:E07957 G13_PAT:E07957 GBPAT:132742 GBPAT:132743 GBPAT:E07957 GB PAT:132742 EM-BA1 :AB003693 GB-BA1 :CGL007732 GBEBAI :CGAMTGEN

E

GB I:-EHCMVCG GB-BA1 :CGL007732 5589 5589 2689 5589 5589 5589 5589 Table 4 (continued) E07957 gDNA encoding at feast guanosine triphosphate cyclohydrolase and riboflavin Corynebacterium synthase. ammoniagenes 132742 Sequence 1 from patent US 5589355. Unknown.

132743 Sequence 2 from patent US 55893.55. Unknown.

AB003693 Corynebacterium ammoniagenes DNA for rib operon, complete ccds. Corynebacteriumn ammoniagenes 132742 132742 AB003693 Sequence 1 from patent US 5589355.

Sequence 1 from patent US 5589355.

Corynebacterium ammoniagenes DNA for rib operon, complete cds.

Unknown.

Unknown.

Carynebacteriumn ammoniagenes 52,909 29-Sep-97 52,909 6-Feb-97 57,937 6-Feb-97 57,937 03-OCT- 1997 (Rel.

52, Created) 57,937 6-Feb-97 61,843 6-Feb-97 61,843 03-OCT- 1997 (ReL 52, Created) 61,843 29-Sep-97 64,346 29-Sep-97 5589 E07957 gDNA encoding at feast guanosine triphosphate cyclohydrolase and riboflavin Corynebacteriumn synthase. ammoniagenes 5589 E07957 gDNA encoding at least guanosine triphosphate cyclohydrolase and riboflavin Corynebacteriumn synthase. ammoniagenes 5589 132742 Sequence 1 from patent US 5589355. Unknown.

2689 132743 Sequence 2 from patent US 5589355. Unknown.

5589 E07957 gDNA encoding at least guanosine triphosphate cyclohydrolase and riboflavin Corynebacteriumn synthase. ammoniagenes 5589 132742 Sequence 1 from patent US 5589355. Unknown.

5589 AB003693 Corynebacterium ammoniagenes DNA for rib operon, complete cds. Corynebacterium 64,346 64,346 56,318 6-Feb-97 6-Feb-97 29-Sep-97 4460 2028 2293 4460 ammoniagenes AJ007732 Corynebacterium glutamicum 3 ppc gene, secG gene, amt gene. ocd gene and 5' soxA gene.

X9351 3 C.glutamicum amt gene.

5.4 X17403 AJO07732 Human cytomnegalovirus strain AD169 complete genome.

Corynebacterium glutamicum 3' ppc gene, secG gene, amt gene, ocd gene and 5'soxA gene.

Corynebacterium glutamicum 3 ppc gene, secG gene, amt gene, ocd gene and 5 soxA gene.

Corynebacteriurn glutamicurn Corynebacterium glutamicum human herpesvirus 5 Corynebacterium glutamicurn Corynebacterium glutamicum GBBAl :CGL007732 4460 AJ007732 56,318 6-Feb-97 56,318 03-OCT- 1997 (Rel.

52. Created) 100,000 7-Jan-99 100,000 29-MAY- 1996 38,651 10-Feb-99 100,000 7-Jan-99 37,526 7-Jan-99 96,928 08-OCT- 1997 (Rel.

52, Created) 96,781 7-Aug.98 36,264 20-Feb-99 36,197 17-MAR- 1999.-?.

IX202272 1368 EMPAT:E09373 GBBA11:D38505 GB..HTG2:AC006595 rxa02281 1545 GBGSS12:AQ411O1 0 1591 E09373 Creatinine dleiminase gene. Bacillus sp.

1591 146070 551 D38505 AC006595 AQ41 1010 Bacillus sp. gene for creatinine deaminase, complete cds.

Homo sapiens, -SEQUENCING IN PROGRESS 4 unordered pieces.

HS_2257 Bi HO2-MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2257 CoI=3 Row--P, genomic survey sequence.

Bacillus sp.

Homo sapiens Homo sapiens 2006200807 24 Feb 2006 GBEST23:A1128623 363 GBPL2:ATACO07O19 102335 GB-BA2:AF1 16184 540 GBGSS9:AQ164310 507 A1128623 AC007019 AF116184 A01 64310 rxa02299 531 rxaO23ll1 813 rxa02315 1752 rxa02318 402 rxa02319 1080 GBV1/:MH68TKH 4557 X93468 GBHTG4:AC006091 176878 AC006091 GB-HTG4:AC006091 176878 AC006091 GBBA2:RRU65510 16259 U65510 Table 4 (continued) qa62c~l.sl Soaresjfetal-heartNbHH19W Homo sapiens cONA clone Homo sapiens IMAGE: 1691328 3, mRNA sequence.

Arabidopsis thaliana chromosome 11 BAG F7D8 genomic sequence, complete Arabidopsis thaliana sequence.

Corynebacterium glutamicum L-aspartate-alpha--decartboxylase precursor Corynebacterium (panD) gene, complete cds. glutamicum HS-27_A2_EO1_MR CIT Approved Human Genomic Sperm Library D Homo sapiens H-omo sapiens genomic clone Plate=2171 001=2 Row--l, genomic survey sequence.

Murine herpesvirus type 68 thymidine kinase and glycoprotein H genes. munine herpesvirus 68 Drosophila melanogaster chromosome 3 clone BACR48GO5 (D475) RPCI-98 Drosophila melanogaster 48.G.5 map 91 F1-91 Fl3 strain y; cn bw sp, **SEQUENCING IN PROGRESS 4 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACR48GO5 (0475) RPCI-98 Drosophila melanogaster 48.G.5 map 91F1-91F13 strain y; cn bw sp, -SEQUENCING IN PROGRESS 4 unordered pieces.

Rhodospirillum rubrum CO-induced hydrogenase operon (cooM, cooK, cool., Rhodospirillum rubrum cooX, cooU, coo-) genes, iron sulfur protein (cooF) gene, carbon monoxide dehydrogenase (cooS) gene, carbon monoxide dehydrogenase accessory proteins (cooC, cooT, cooJ) genes, putative transcriptional activator (cooA) gene, nicotinate-nucleotide pyrophosphorylase (nadC) gene, complete cds, L-aspartate oxidase (nadB) gene, and alkyl hydroperoxide reductase (ahpC) gene, partial cds.

Mycobacterium tuberculosis sequence from clone y224. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 28/162- Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone y 22 4. Mycobacteriumn tuberculosis Homo sapiens chromosome 5 clone CIT-HSPC_303EI3, SEQUENCING Homo sapiens IN PROGRESS 3 ordered pieces.

Homo sapiens chromosome 5 clone CIT-HSPC 303E13, SEQUENCING Homo sapiens IN PROGRESS 3 ordered pieces.

Homo sapiens chromosomeS5 clone CIT978SKB_81 K21, SEQUENCING Homo sapiens IN PROGRESS 3 ordered pieces.

Mycobacterium tuberculosis sequence from clone y224. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 281162. Mycobacterium tuberculosis ub83h102.rl Soares 2NbMT Mus musculus cONA clone IMAGE:1395123 Mus musculus sequence.

37,017 1998 33,988 16-MAR- 1999 100,000 02-MAY- 1999 37,278 16-OCT- 1998 40.288 3-Sep-96 36,454 27-OCT- 1999 36,454 27-OCT- 1999 37,828 9-Apr-97 GB...BA1 :MSGY224 GB-BA1:MTY25D1O GBBA1;M5GY224 GBHTG3:AC01 1348 GB-TG3:ACO1 1348 GBHTG3:AC01 1412 GB.BA1 :MSGY224 GBBA1:MTY25DIO GB-EST23:Al1 17213 40051 40838 40051 111083 111083 89234 40051 40838 476 AD000004 Z95558 A0OOO004 AC01 1348 AC01 1348 AC01 1412 AD000004 Z95558 All 17213 49,418 49,360 38,150 35,821 35,821 36.181 37.792 37,792 35,084 03-DEC- 1996 1 7-Jun-98 03-DEC- 1996 06-OCT- 1999 06-OCT- 1999 06-OCT- 1999 03-DEC- 1996 17-Jun-98 2-Sep-98" 2006200807 24 Feb 2006 rxa02345 1320 GBBA1:BAPURKE GBBA1:MTCY71 GBBAI:MTCY71 rxa02350 618 GB-BAI:BAPURKE GBPL1:SC13OKBXV Gb'PL1 :SCXVORFS rxa02373 1038 GBPAT:E00311 GBPAT:106030 GB-PAT:100836 rxa02375 1350 GBBA2:CGU31230 rxa02380 777 rxa02382 1419 rxa02400 693 rxa02432 1098 GBHTG3:AC009946 GBHTG3:AC009946 GB-BAI:MTCY253 GBHTG4:AC010658 GB-HTG4:AC010658 G8_BA1:CGPROAGE

N

GB.BAI :MTCY428 GBBA2:CGU31230 GBBA1:CGACEA GB PAT:1861 91 GBPAT:113693 GBGSS15:AQ60684 2 2582 42729 42729 2582 129528 50984 1853 1853 1853 3005 169072 169072 41230 1 20754 120754 1783 26914 3005 2427 2135 2135 574 X91189 Z92771 Z92771 X91 189 X94335 X90518 E00311 106030 100836 U31230 AC009946 AC009946 Z81368 AC01 0658 AC01 0658 X82929 Z81451 U31230 X75504 186191 113693 AQ606842 Table 4 (continued) Bammoniagenes purK and purE genes. Corynebacteriumn ammoniagenes Mycobacteriumn tuberculosis H37Rv complete genome; segment 141/162. Mycobactenium tuberculosis Mycobacteriumn tuberculosis H37Rv complete genome; segment 141/1 62. Mycobacterium tuberculosis Bammoniagenes purK and purE genes. Corynebacterium ammoniagenes S.cerevisiae 130kb DNA fragment from chromosome XV. Saccharomyces cerevisiae S.cerevisiae DNA of 51 Kb from chromosome XV right arm. Saccharomyces cerevisiae DNA coding of 2,5-diketogluconic acid reductase. unidentified Sequence 4 from Patent EP 0305608. Unknown.

Sequence 1 from Patent US 4758514. Unknown.

Corynebactenium glutamicum Obg protein homolog gene, partial cdls, gamma Corynebacterium glutamyl kinase (proB) gene, complete cds, and (unkdh) gene, complete cds. glutamicumn Homo sapiens clone NHOOI2C17. SEQUENCING IN PROGRESS 1. Homo sapiens unordered pieces.

Homo sapiens clone NHOO12C17, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 106/1 62. Mycobacterium tuberculosis Drosophila melanogaster chromosome 3LJ75C1 clone RPCI98-3B20, Drosophila melanogaster SEQUENCING IN PROGRESS 78 unordered pieces.

Drosophila melanogaster chromosome 3L75C1 clone RPCI98-3B20, Drosophila melanogaster SEQUENCING IN PROGRESS 78 unordered pieces.

C.glutamicum proA gene. Corynebacteriumn glutamicum Mycobacterium tuberculosis H-37Rv complete genome; segment 107/1 62. Mycobacteriumn tuberculosis Corynebacterium glutamnicumn Obg protein homolog gene, partial cds, gamma Corynebacterium glutamyl kinase (proB) gene, complete cds, and (unkdh) gene, complete cds. glutamicum C.glutamicum aceA gene and thiX genes (partial). Corynebacterium glutamicumn Sequence 3 from patent US 5700661. Unknown.

Sequence 3 from patent US 5439822. Unknown.

HS -5404_-B2_-E07_-T7A RPCI-1 1 Human Male BAC Library Homo sapiens Homo sapiens genomic; clone Plate=980 Col=14 Row=J, genomic survey sequence.

61,.731 39,624 39,847 64,286 36,617 36,617 56,123 56,220 56,220 99,332 36.115 :36,115 38,088 35,817 35,817 98,802 38,054 98,529 100,000 100.000 100,000 39,716 14-Jan-97 10-Feb-99 1 0-Feb-99 14-Jan-97 15-Jul-97 1 29-Sep-97 02-DEC- 1994 21-MAY- 1993 2-Aug-96 8-Sep-99 8-Sep-99 17-Jun-98 16-OCT- 1999 16-OCT- 1999 23-Jan-97 17-Jun-98 2-Aug-96 9-Sep-94 10-Jun-98 26-Se 110-Jurff99 2006200807 24 Feb 2006 rxa02458 1413 GBEST1:T05804 406 GB-P1:AB006699 77363 GBBA2:AF1 14233 1852 GB-EST37:AWO1 306 578 GB-GSS15:AQ65002 728 7 GB-BA1 :MTCY359 36021 Table 4 (continued) T05804 EST03693 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA clone Homo sapiens HFBDG63 similar to EST containing Alu repeat, mRNA sequence.

AB006699 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone: MDJ122, Arabidopsis thaliana complete sequence.

AFi 14233 Corynebacterium glutamicum 5-enolpyruvylshikimate 3-phosphate synthase Corynebacterium (aroA) gene, complete cds. glutamicum AW013061 ODT-0033 Winter flounder ovary Pleuronectes americanus cDNA clone OT- Pleuronectes americanus 0033 5' similar to FRUCTOSE-BISPHOSP-ATE ALDOLASE B (LIVER), mnRNA sequence.

AQ650027 Sheared DNA-5L2.TF Sheared DNA Trypanosoma brucel genomic clone Trypanosoma brucei Sheared DNA-5L-2, genomic survey sequence.

Z83859 Mycobacterium tuberculosis H37Rv complete genome; segment 84/162. Mycobacterium 37,915 30-Jun-93 35,526 20-Nov-99 100,000 7-Feb-99 39.175 10-Sep-99 rxa02469 1554 rxa02497 1050 GB BAI:MLCBI788 GBBAI:SCAJ106O1 GB_8A2:CGU31224 39228 4692 422 AL008609 AJO1 0601 U31 224 GB-BA1:MTCY2OG9 37218 Z77162 GBBAl :SCE7 16911 AL049819 tuberculosis Mycobacterium leprae cosmid B1788. Mycobacterium leprae Streptomyces coelicalor A3(2) DNA for whiD) and whiK loci. Streptomyces coelicolor Corynebacterium glutamicum (ppx) gene, partial cds. Corynebacterium glutamicum Mycobacterium tuberculosis H37Rv complete genome; segment 251162. Mycobacterium tuberculosis Streptomyces coelicolor cosmid ET. Streptomyces coelicolor Corynebacterium glutamicum L-proline:NADP+ 5-oxidoreductase (proC) gene, Corynebacterium complete cds. glutemicum Neisseria gonorrhoeae pilA gene. Neisseria gonorrhoeae Drosophila melanogaster chromnosome 3 clone BACRO5C1O (D781) RPCI-98 Drosophila melanogaster 05.C.10 map 97D-97E strain y; cn bw sp. **SEQUENCING IN PROGRESS -,87 unordered pieces.

Mycobacterlum tuberculosis H37Rv complete genome; segment 25/162. Mycobacterium 39,281 22-Jun-99 39,634 17-Jun-98 59.343 27-Aug-99 48,899 17-Sep-98 96,445 2-Aug-98 59,429 17-Jun.98 39,510 1999 97,749 2-Aug-96 rxa02499 933 GBBA2:CGU31225 1817 U31225 GBBA1:NG17PILA 1920 X13965 GBHTG2:AC007984 129715 AC007984 GB-BA1:MTCY20G9 37218 Z77162 33,406 39,357 2-Aug-99 17-Jun-98 rxa02501 1188 rxa02503 522 rxaO2504 681 GBBA1:U0001 8 GBVI:HE1CG GBPR3:AC005328 GS PR3:AC005545 GB PR3:AC005328 GBBA1:MTCY2OG9 152261 35414 43514 35414 37218 35414 43514 36224 X14112 AC005328 AC005545 AC005328 Z77162 AC005328 AC005545 Z99125 Herpes simplex virus (HSV) type 1 complete genome.

Homo sapiens chromosome 19, cosniid R26660, complete sequence.

Homo sapiens chromosome 19, cosmid R26634, completi sequence.

Homo sapiens chromosome 19, cosmid R26660, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 25/1 62.

Homo sapiens chromosome 19, cosmid R26660, complete sequence.

Homo sapiens chromosome 19, cosmid R26634, complete sequence.

Mycobacterium leprae cosmid 1536.

42991 U00018 Mycobacterium leprae cosmid B2168.

tuberculosis Mycobacterium leprae human herpesvirus 1 Homo sapiens Homo sapiens Homo sapiens Mycobacterium tuberculosis Homo sapiens Homo sapiens Mycobacterium leprae Mycobacterium leprae 51,768 01-MAR- 1994 39,378 17-Apr-97 39,922 28-Jul-98 39,922 3-Sep-98 34,911 28-Jul-98 54,940 17-Jun-98 41,265 28-Jul-98 41,265 3-Sep-98 37,723 04-DEC- 1998 37,723 01-MAR- 1994 GB-PR3:AC005328 GBPR3:AC005545 rxaO2516 1386 GB-BAI:MLCL536 GBBA1:U00013 35881 U0001 3 Mycobacterium leprae cosmid 81496.

2006200807 24 Feb 2006 GB-BAl :MTVOO7 rxa02517 570 GBBA1:MLCL536 GB_BA1:U00013 GB..BA1 :SCC22 rxa02532 1170 GB OV:AF137219 GBEST3O:A1645057 GBEST2O:AA822595 rxa02536 879 GB-HTG2:AF130866 GBHTG2:AF1 30866 GBPLI:ATT12J5 rxaO2550 1434 GBBA1:MTCY279 GBBA1:MSGBI97OC

S

GBBA2:SC2H4 rxa02559 1026 GBBA1:MTVOO4 GBPAT:128684 GBBA1:MTU27357 rxa02622 1683 GB-BA2:AE001 780 GBOV:AF064564 32806 36224 35881 AL021 184 Z991 25 U00013 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome: segment 64/162.

Mycobacterium leprae cosmid L536.

Mycobacterium leprae cosmid B1496.

Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae 22115 AL096839 Streptomyces coelicolor cosmid C22. Streptomyces coelicolor 831 AF137219 Amia calva mixed lineage leukemia-like protein (MII) gene, partial cds. Amia calva 301 A1645057 vsS2alO.yl Stratagene mouse Tcell 937311 Mus musculus cDNA clone Mus musculus IMAGE: 1149882 mRNA sequence.

429 AA822595 vs52a10.r1 Stratagene mouse Tcell 937311 Mus musculus cDNA clone Mus musculus IMAGE: 149882 mRNA sequence.

118874 AF130866 Homo sapiens chromosome 8 clone PAC 172N1 3 map 8q24, Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

118874 AF130866 Homo sapiens chromosome 8 clone PAC 172N1 3 map 8q24, Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

84499 AL035522 Arabidopsis thaliana DNA chromosome 4, BAC clone T12J15 (ESSAII project). Arabidopsis thaliana 9150 Z97991 Mycobacterium tuberculosis H37Rv complete genome; segment 17/162. Mycobacterium tuberculosis 39399 L78815 Mycobacterium leprae cosniid B1970 DNA sequence. Mycobacteriumn leprae 25970 AL031514 Streptomyces coelicolor cosmid 21-14. Streptomyces coelicolor A3(2) 69350 AL009198 Mycobacterium tuberculosis H37Rv complete genome; segment 144/162. Mycobacterium tuberculosis 5100 128684 Sequence 1 from patent US 5573915. Unknown.

5100 U27357 Mycobacterium tuberculosis cycinpropane mycolic acid synthase (anal) Mycobacterium gene, complete cds. tuberculosis 11997 AE001780 Therrnotoga maritima section 92 of 136 of the complete genome. Thermotoga maritima 49254 AF064564 Fugu rubripes neurofibromatosis type 1 (NFl). A-kinase anchor protein Fugu rubripes (AI<AP84), BAW protein (BAW), and WSB1 protein (WSB1) genes, complete cds.

49254 AF064564 Fugu rubripes neurofibromatosis type 1 (NFlI), A-kinase anchor protein Fugu rubripes (AKAP84), RAW protein (RAW), and WSB1 protein (WSB31) genes, complete cds.

444 AQ818728 HS_526B_Al_G09_SP6E RPCI-11I Human Male BAC Library Homo sapiens H-omo sapiens genomic clone Plate=844 Col=17 Row--M, genomic survey sequence.

198586 AC01 1083 Homo sapiens chromosome 9 clone RP1 1-111 M7 map 9, WORKING DRAFT Homo sapiens SEQUENCE, 51 unordered pieces.

544 AQ826948 HS_5014_A2_C12_T7A RPCI-11 Human Male BAC Library Homo sapiens Homo sapiens genomic done Plate=590 Col=24 Row=E. genomic survey sequence.

61,.335 37,018 37,018 37,071 36,853 41,860 42,353 40,754 40,754 35,063 37,773 39,024 37,906 47.358 39,138 39.138 44,914 39,732 17-Jun-98 04-DEC- 1998 0 1-MAR- 1994 12-Jul-99 7-Sep-99 29-Apr-99 17-Feb-98 21-MAR- 1999 21-MAR- 1999 24-Feb-99 17-Jun-98 15-Jun-96 1 9-OCT- 1999 18-Jun-98 6-Feb-97 26-Sep-95 2-Jun-99 17-Aug-99 rxa02623 714 GBOV:AF064564 GBG555:AQ818728 1083 GB..GSS6AQ826948 36,703 17-Aug-99 38,801 26-Aug-99 35,714 39,146 19-Nov-99 27-Augt9g 2006200807 24 Feb 2006 rxa02629 708 GBVI:BRSMGP

GBVI:BRSMGP

462 M86652 462 M86652 rxa02645 1953 rxa02646 1392 rxa02648 1326 GB-PAT:A45577 1925 GBPAT:A45581 1925 GB.BA1 :CORILVA 1925 GB-BAI CORILVA 1925 GBPAT:A45585 1925 GBPAT:A45583 1925 GB-OV:ICTCNC 2049 GBEST1 1:AA265464 345 GBGS58:AQ005950 480 A45577 A45581 1-01508 L01508 A45585 A45583 M831 11 AA265464 A0006950 Table 4 (continued) Bovine respiratory syncytial virus membrane glycoprotein mRNA, complete cds.

Bovine respiratory syncytial virus membrane glycoprotein mRNA, complete cds.

Sequence 1 from Patent W09519442.

Sequence 5 from Patent W095 19442.

Corynebacterium glutamicum threonine dehydratase (ilvA) gene, complete cds.

Corynebacterium glutamicum threonine dehydratase (ilvA) gene, complete cds.

Sequence 9 from Patent W09519442.

Sequence 7 from Patent W09519442.

Ictalurus punctatus cyclic nucleotide-gated channel RNA sequence.

mx9lcO6.rl Soares mouse NML Mus musculus cDNA clone IMAGE:693706 mRNA sequence.

CIT-H-SP-2294E14.TR CIT-HSP Homo sapiens genomic clone 2294E1 4.

genomic survey sequence.

C.glutamicum pheA gene encoding prephenate dehydratase. complete cds.

DNA encoding prephenate dehydratase.

DNA encoding prephenate dehydratase.

Hordeum vulgare DNA for chromosome 4H.

Human DNA sequence from cosniid 3101-5 from a contig from the tip of the short arm of chromosome 16, spanning 2Mb of 16p1 3.3. Contains EST and CpG island.

Homo sapiens chromosome 16, cosmid clone RT286 (LAN complete sequence.

Drosophila melanogaster chromosome 3 clone BACR16118 (D815) RPCI-98 16.1.18 map 95A-95A strain y: cn bw sp, SEQUENCING IN PROGRESS-. 101 unordered pieces.

Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Ictalurus punctatus Mus muscutus Homo sapiens 39,130 39,130 39,130 99,138 99,066 99,066 38,402 38,655 36.074 Bovine respiratory syncytiat 37,013 virus Bovine respiratory syncytial 37,013 virus 28-Apr-93 28-Apr-93 07-MAR- 1997 07-MAR- 1997 26-Apr-93 26-Apr-93 07-MAR- 1997 07-MAR- 1997 24-MAY- 1993 1997 27-Jun-98 rxa02653 rxa02687 1068 rxaD2717 1005 rxa02754 1461 GBBA1:CORPHEA GBPAT:E04483 GB,.PAT:E061 10 GBPL1:HVCH4- GB-PR2:HS310H5 GByPR3AC04754 1088 948 948 59748 29718 M13774 E04483 E061 10 Y14573 Z69705 Corynebacterrum glutamicum Corynebacterium glutamicumn Corynebacterium glutamicum Hordeum vulgare Homo sapiens Homo sapiens Drosophila melanogaster 99,715 98,523 98,523 36,593 36.089 26-Apr-93 29-Sep-97 29-Sep-97 1999 22-Nov-99 39188 AC004754 36.089 28-MAY- 1998 GB-HTG2:AC008223 130212 AC008223 32,757 2-Augt99 2006200807 24 Feb 2006 GB-HTG2:AC008223 130212 AC008223 rxa02758 1422 rxa02771 678 rxa02772 1158 rxa02790 1266 rxa02791 951 rxa028O2 1194 GB_BA1:MTCY71 GBHTG5:AC011678 GBHTG5:AC011678 GBBA2:AF064070 GBBA2:AF038651 GBIN1:CELT19B4 GB-EST36:AV193572 GBBA2:AF038651 GB_BAl :MTCY227 GB_BA1:U00011I GB_BA1:MTCY159 GBPR4:AC006581 GBPR4:AC006581 GBBA1 :MTCYl 59 GB_OV:CHKCEK2 GBBAI :MSASDASK GBEST24:A1223401 42729 171967 171967 23183 Z92771 AC01 1678 AC01 1678 AF064070 4077 AF038651 37121 360 4077 35946 40429 33818 172931 172931 33818 3694 5037 169 U80438 AV1 93572 AF038651 Z77724 U000ll Z83863 AC006581 AC006581 Z83863 M35195 Z17372 A1223401 Table 4 (continued) Drosophila melanogaster chromosome 3 clone BACRI1I18 (D815) RPCI-98 16.1.18 map 95A-95A strain y; cn bw sp, SEQUENCING IN PROGRESS -,101 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 141/162.

H-omo sapiens dlone 14_B_7, SEQUENCING IN PROGRESS ~,20 unordered pieces.

Homo sapiens clone 14_B_7, SEQUENCING IN PROGRESS ~,20 unordered pieces.

Burkholderia pseudomallei putative dihydroorotase (pyrC) gene, partial cds*, putative 1 -acyl-sn-glycerol-3-phosphate acyltransferase (plsC), putative diadenosine tetraphosphatase (apaH), complete cds; type 11 0-antigen biosynthesis gene cluster, complete sequence; putative undecaprenyl phosphate N-acetylg lucosaminyltra nsfe rase, and putative UDP-glucose 4epimerase genes, complete cdls; and putative galactosyl transferase gene, partial cds.

Corynebacterium glutamicum dipeptide-binding protein (dciAE) gene, partial cds; adenine phosphoribosyltra nsfe rase (apt) and GTP pyrophosphokinase (ret) genes, complete cds; and unknown gene.

Caenorhabditis elegans cosmid T19134.

AV1 93572 Yuji Kohara unpublished cDNA:Strain N2 hermaphrodite embryo Caenorhabditis elegans cDNA clone yk618h8 mRNA sequence.

Corynebacterium glutamicum dipeptide-binding protein (dciAE) gene, partial cds; adenine phosphoribosyltransferase (apt) and GTP pyrophosphokinase (rel) genes, complete cds; and unknown gene.

Mycobacterium tuberculosis H-37Rv complete genome; segment 114/162.

Mycobacterium leprae cosmid B1 177.

Mycobacterium tuberculosis H37Rv complete genome; segment 111/162.

Homo sapiens 12p2l BAC RPC11-259018 (Roswell Park Cancer Institute Human BAC Library) complete sequence.

Homo sapiens 12p21 BAC RPClI11-259018 (Roswell Park Cancer Institute Human BAC Library) complete sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 111/162.

Chicken tyrosine kinase (cek2) mRNA. complete cds.

M.smegmatis asd, ask-alpha. and ask-beta genes.

qg48g01.xl SoaresjestisNHT Homo sapiens cDNA clone IMAGE:1838448 3' similar to WP:C25D7.8 CE08394 mRNA sequence.

Drosophila melanogaster Mycobacterium tuberculosis Homo sapiens Homo sapiens Burkholderia pseudomallei Corynebacteriumn glutamicum Caenorhabditis elegans, Caenorhabditis elegans Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium tuberculosis Homo sapiens Homo sapiens Mycobacterium tuberculosis Gallus gallus Mycobacteriumn smegmatis Homo sapiens 32,757 2-Aug-99 37,838 10-Feb-99 35,331 5-Nov-99 33,807 5-Nov-99 36,929 20-Jan-99 99,852 14-Sep-98 43,836 48.588 99,914 38,339 38,996 37,840 37,906 35.280 39,765 38,937 38,495 40,828 04-DEC- 1996 22-Jul-99 14-S ep-98 17-Jun-98 01-MAR- 1994 17-Jun-98 3-Jun-99 3-Jun-99 17-Jun-98 28-Apr-93 9-Aug-94 27-Oct- 1998 2006200807 24 Feb 2006 GBEST24:A1223401 169 A1223401 rxa02814 494 rxa02843 608 rxs03205 963 rxs03223 1237 GB_BAI:MTCY7D11 22070 GB-BA1 :MTCY7Dl 1 22070 GBPR1:HSAJ2962 778 GBBA1:CGAJ4934 1160 GBBA1:MTCI3B4 29540 GBBAI:MLU1518O 38675 GB BA1:BLSIGBGN 2906 GB-EST21 :AA980237 377 Z95120 Z95120 AJ002962 AJO04934 Z93777 U15180 Z49824 AA980237 A1158316 AL031910 AL09671 0 Table 4 (continued) qg48gOl.xl Soares testis_-NHT Homo sapiens cONA clone IMAGE: 1838448 Homo sapiens 3' similar to WVP:C25D7.8 CE08394 mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 138/162. Mycobacteriunm tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 138i162. Mycobacteriuntuberculosis Homo sapiens mRNA for hB-FABP. Homo sapiens Corynebacterium glutamnicum dapl) gene, complete CDS. Corynebacteriu glutamnicum Mycobacterium tuberculosis H-37Rv complete genome; segment 52/162. Mycobacteriur tuberculosis Mycobacterium Ieprae cosmid B1 756. Mycobacteriumr B.Iaclofermentumn orfi gene and sigB gene. Corynebacteri.

ua32a1 2.rl Soares-mammary~gand NbMMG Mus musculus cONA clone Mus musculus IMAGE:1 348414 5' similar to TR:Q61025 061025 HYPOTHETICAL 15.2 KO PROTEIN. mRNA sequence.

ud27c05.rl Soaresthymus_2NbMT Mus musculus cDNA clone -Mus musculus IMAGE:1447112 mRNA sequence.

Leishmania major Friedlin chromosome 4 cosmid L2743. Leishmania nn Human DNA sequence from done RP1-61B2 on chromosome 6p1 1.2.12.3 Homo sapiens Contains isoforms 1 and 3 of BPAG1 (bullous pemphigoid antigen 1 (230/2400D), an exon of a gene similar to murine MACIF cytoskeletal protein, STSs and GSSs, complete sequence.

Human DNA sequence from clone RPI -61 B2 on chromosome 6p1 1.2-12-3 Homo sapiens Contains isoforms 1 and 3 of BPAG1 (bullous pemphigoid antigen 1 (230I240kD), an exon of a gene similar to murine MACF cytoskeletal protein, STSs and GSSs, complete sequence.

leprae nm glutamicu 40,828 27-OCT- 1998 58,418 17-Jun-98 40,496 17-Jun-98 39,826 8-Jan-98 100,000 17-Jun-98 37,710 17-Jun-98 39,626 09-MAR- 1995 r08,854 25-Apr.96 41,489 27-MAY- 1998 38,005 30-Sep--98 39,869 1999 34,930 17-DEC- 1999 34,634 17-DEC.

1999 GBEST23:A[158316 GBI[N1 :LMFL2743 GBPR3:HSDJ61B2 371 38368 119666 tjor GBPR3:HSDJ6IB2 119666 AL096710 132- CN Exemplification SExample 1: Preparation of total genomic DNA of Corynebacterium glutamicum (c ATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by 00 O centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml Sbuffer-I of the original volume of the culture all indicated volumes have been D calculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/l sucrose, .2.46 g/l MgSO, x 7H 2 0, 10 ml/l KHPO, solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/l M12 concentrate (10 g/l (NH 4 1 g/1 NaCI, 2 g/l MgSO x 7H 2

O,

0.2 g/1 CaCIl, 0.5 g/l yeast extract (Difco), 10 ml/1 trace-elements-mix (200 mg/1 FeSO, x H 2 0, 10 mg/l ZnSO, x 7 H20, 3 mg/I MnCl, x 4 HzO, 30 mg/l H 3 BO, 20 mg/1 CoCl, x 6 HO 2 1 mg/l NiCI 2 x 6 HzO, 3 mg/1 NaMoO, x 2 HO 2 500 mg/l complexing agent (EDTA or critic acid), 100 ml/I vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/l p-amino benzoic acid, 20 mg/1 riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/I pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37'C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCI, 1 mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCI solution (5 M) are added. After adding ofproteinase K to a final concentration of 200 4g/ml, the suspension is incubated for ca. 18 h at 37 0 C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing tg/ml RNaseA and dialysed at 4 0 C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCI and 0.8 ml of ethanol are added. After a 0 min incubation at -20 0 C, the DNA was collected by centrifugation (13,000 rpm, S Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in STE-buffer. DNA prepared by this procedure could be used for all purposes, including southern blotting or construction of genomic libraries.

Example 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC13032 0 Using DNA prepared as described in Example 1, cosmid and plasmid libraries were constructed according to known and well established methods (see I Sambrook, J. et al. (1989) "Molecular Cloning A Laboratory Manual", Cold 10 Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons).

Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741); pACYC177 (Change Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene, LaJolla, USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL109 (Lee, and A.J. Sinskey (1994) J. Microbiol. Biotechnol. 4:256-263).

Example 3: DNA Sequencing and Computational Functional Analysis Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using AB1377 sequencing machines (see Fleischman, R.D. et al.

(1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496-512). Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3' (SEQ ID NO.

1157) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID NO. 1158).

Example 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain -134- CN the integrity of their genetic information. Typical mutator strains have mutations in the genes for the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM: CN Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

00 SExample 5: DNA Transfer Between Escherichia coli and Corynebacterium Sglutamicum SSeveral Corynebacterium and Brevibacterium species contain endogenous S 10 plasmids (as pHM1519 or pBL1) which replicate autonomously (for review see, e.g., Martin, J.F. el al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vectors into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schifer, A et al.

135- C1 (1990) J. Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for SC. glutamicum to E. coli by preparing plasmid DNA from C. glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step Cl can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983)J. Mol. Biol. 166:1-19).

Genes may be overexpressed in C. glutamicum strains using plasmids which Scomprise pCGI Patent No. 4,617,267) or fragments thereof, and optionally the gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980) SProc. Nail. Acad. Sci. USA 77(12): 7176-7180). In addition, genes may be 10 overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

136- (1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer Sdesigned to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity O of mRNA for this gene. This information is evidence of the degree of transcription of the O mutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by

O

N1 several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

S(1992) Mol. Microbiol. 6:317-326.

O

N1 10 To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989)Appl. Microbiol. Biolechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be -137- CN advantageous to supply mixtures of different carbon sources. Other possible carbon Ssources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials C, which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NH,C1 or (NH,) 2 SO,, NHOH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast 00 extract, meat extract and others.

C Inorganic salt compounds which may be included in the media include the Schloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, 10 molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121 or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in a range between 15"C and 45C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It 138- (N is also possible to maintain a constant culture pH through the addition of NaOH or SNHOH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex r compounds have high buffer capacities. Ifa fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

The incubation time is usually in a range from several hours to several days. This 00 time is selected in order to permit the maximal amount of product to accumulate in the Sbroth. The disclosed growth experiments can be carried out in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 6 00 of 0.5 1.5 using cells grown on agar plates, such as CM plates (10 g/l glucose, 2,5 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30°C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities may be 139- CN found, for example, in the following references: Dixon, and Webb, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San CI Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 r d ed. Academic Press: New r York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 00 OO 3527300325); Bergmeyer, Bergmeyer, GraB1, eds. (1983-1986) Methods of Enzymatic Analysis, 3 rd ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's SEncyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical chromatography such as high performance liquid chromatography (see, for example, -140- (N Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: SWeinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al.

CO1 (1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

00 (1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of SIndustrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum 141 r, cells, then the cells are removed from the culture by low-speed centrifugation, and the Ssupernate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the 0 impurities are retained by the resin while the sample is not. Such chromatography steps 00 0may be repeated as necessary, using the same or different chromatography resins. One C of ordinary skill in the art would be well-versed in the selection of appropriate 0chromatography resins and in their most efficacious application for a particular molecule 1 10 to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA -142- CN 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100, C wordlength 12 to obtain nucleotide sequences homologous to MP nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score 50, wordlength 3 to obtain amino acid sequences Shomologous to MP protein molecules of the invention. To obtain gapped alignments for Scomparison purposes, Gapped BLAST can be utilized as described in Altschul et al., N (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program XBLAST and NBLAST) for the specific sequence being analyzed.

Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4: 11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention -143- CN were compared to genes present in Genbank in a three-step process. In a first step, a SBLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the CI top 500 hits were retained for further analysis. A subsequent FASTA search a combined local and global alignment analysis, in which limited regions of the sequences are aligned) was performed on these 500 hits. Each gene sequence of the invention was Ssubsequently globally aligned to each of the top three FASTA hits, using the GAP program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the 10 length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 12: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label -144- C1 may be used to monitor and measure the individual signal intensities of the hybridized Smolecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic C acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., Schena, M. (1996) BioEssays 18(5): 427-431).

SThe sequences of the invention may be used to design oligonucleotide primers Swhich are able to amplify defined regions of one or more C. glutamicum genes by a Snucleic acid amplification reaction such as the polymerase chain reaction. The choice I 10 and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as -145- CN described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for Sexample, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray CI technology, as described above, permits comparative analyses of different strains of C.

glutamicum or other Corynebacteria. For example, studies of inter-strain variations based on individual transcript profiles and the identification of genes that are important 00 Sfor specific and/or desired strain properties such as pathogenicity, productivity and i stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons Sof the profile of expression of genes of the invention during the course of a fermentation 10 reaction are possible using nucleic acid array technology.

Example 13: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et al. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: -146- (N 1451-1463). Other separation techniques may also be utilized for protein separation, Ssuch as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard C techniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein 0 precursors 35 S-methionine, 35 S-cysteine, 4 C-labelled amino acids, 1 N-amino N acids, 5

NO

3 or I 5

NH

4 or 13C-labelled amino acids) in the medium of C. glutamicum

ID

permits the labeling of proteins from these cells prior to their separation. Similarly, fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

EQUIVALENTS

(Ni Those of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

00oo Comprises/comprising and grammatical variations thereof when used in 0 this specification are to be taken to specify the presence of stated features, IND integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.

Claims (21)

1. 3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.
2. 4. An isolated nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof. An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid molecule does not consist of any of the F- designated genes set forth in Table 1, or a complement thereof.
3. 6. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims 1-5 and a nucleotide sequence encoding a heterologous polypeptide.
4. 7. A vector comprising the nucleic acid molecule of any one of claims 1-6.
5. 8. The vector of claim 7, which is an expression vector.
6. 9. A host cell transfected with the expression vector of claim 8. The host cell of claim 9, wherein said cell is a microorganism. S11. The host cell of claim 10, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.
7. 12. The host cell of claim 9, wherein the expression of said nucleic acid molecule results 00 Sin the modulation in production of a fine chemical from said cell. O IN
8. 013. The host cell of claim 12, wherein said fine chemical is selected from the group C consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.
9. 14. A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.
10. 16. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.
11. 17. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 1155, provided that the nucleic acid Smolecule does not consist of any of the F-designated nucleic acid molecules set forth Sin Table 1. N 18. An isolated polypeptide comprising an amino acid sequence which is at least identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: r"- 0 4 to 1156, provided that the amino acid sequence is not encoded by any of the F- 00 0designated genes set forth in Table 1. O S19. An isolated polypeptide comprising a fragment of a polypeptide comprising the amino c acid sequence of any of the even numbered SEQ ID NOs: 4 to 1156, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence. The isolated polypeptide of any of claims 15-19, further comprising heterologous amino acid sequences.
12. 21. A method for producing a fine chemical, the method comprising culturing the cell of claim 9 such that the fine chemical is produced.
13. 22. The method of claim 21, wherein said method further comprises the step of recovering the fine chemical from said culture.
14. 23. The method of claim 21, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.
15. 24. The method of claim 21, wherein said cell is selected from the group consisting of: Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Ni Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, SBrevibacterium butanicum, Brevibacterium divaricatum, Brevibacterium flavum, C"1 Brevibacterium healii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium linens, a Brevibacterium paraffinolyticum, and those strains set forth in Table 3. 00 C1 25. The method of claim 21, wherein expression of the nucleic acid molecule from said 0 vector results in modulation of production of said fine chemical.
16. 26. The method of claim 21, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.
17. 27. The method of claim 21, wherein said fine chemical is an amino acid.
18. 28. The method of claim 27, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, methionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, phenylalanine, and tryptophan.
19. 29. A method for producing a fine chemical, the method comprising culturing a cell whose genomic DNA has been altered by the introduction of a nucleic acid molecule of any one of claims 1-6. A method for diagnosing the presence or activity of Corynebacterium diphtheriae in a subject, the method comprising detecting the presence of at least one of the nucleic acid molecules of any one of claims 1-5 or the polypeptide molecules of any one of F 152 \NO claims 15-19, thereby diagnosing the presence or activity of Corynebacterium 0 diphtheriae in the subject. S31. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, wherein the nucleic acid molecule is disrupted. 0 32. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, wherein the nucleic acid molecule (NO comprises one or more nucleic acid modifications as compared to the sequence set C forth in any of the odd-numbered SEQ ID NOs: 3 to 1155.
20. 33. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 1155, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.
21. 34. A polypeptide produced by the method of claim 14. A fine chemical produced by the method of any one of claims 21 -29. DATED this 24 th day of February 2006 BASF AKTIENGESELLSCHAFT WATERMARK PATENT TRADE MARK ATTORNEYS 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA seql i st cor r ect ed. t xt SEQUENCE LI STI NG <110> BASF Akt i engesel I schaf t <120> CORYNEBACTERI UM GLLTAM CUM GENES ENCODI NG VETABOLI C PATHV\Y PROTEI NS <130> BGI-121CPPC <140> PCT/001 B/00923 <141> 2000-06-23 <150> US 60/141,031 <151> 1999-06-25 <150> US 60/142, 101 <151> 1999-07-02 <150> US 60/148,613 <151> 1999-08-12 <150> US 60/187,970 <151> 2000-03-09 <150> DE 19930476.9 <151> 1999-07-01 <150> DE 19931415.2 <151> 1999-07-08 <150> DE 19931418.7 <151> 1999-07-08 <150> DE 19931419.5 <151> 1999-07-08 <150> DE 19931420.9 <151> 1999-07-08 <150> DE 19931424.1 <151> 1999-07-08 <150> DE 19931428.4 <151> 1999-07-08 <150> DE 19931434.9 <151> 1999-07-08 <150> DE 19931435.7 <151> 1999-07-08 <150> DE 19931443.8 <151> 1999-07-08 <150> DE 19931453.5 <151> 1999-07-08 <150> DE 19931457.8 <151> 1999-07-08 <150> DE 19931465.9 <151> 1999-07-08 <150> DE 19931478.0 <151> 1999-07-08 <150> DE 19931510.8 <151> 1999-07-08 Page 1 seql i st cor r ect ed. t xt <150> DE 19931541.8 <151> 1999-07-08 <150> DE 19931573.6 <151> 1999-07-08 <150> DE 19931592.2 <151> 1999-07-08 <150> DE 19931632.5 <151> 1999-07-08 <150> DE 19931634. 1 <151> 1999-07-08 <150> DE 19931636.8 <151> 1999-07-08 <150> DE 19932125.6 <151> 1999-07-09 <150> DE 19932126.4 <151> 1999-07-09 <150> DE 19932130.2 <151> 1999-07-09 <150> DE 19932186.8 <151> 1999-07-09 <150> DE 19932206.6 <151> 1999-07-09 <150> DE 19932227.9 <151> 1999-07-09 <150> DE 19932228.7 <151> 1999-07-09 <150> DE 19932229.5 <151> 1999-07-09 <150> DE 19932230.9 <151> 1999-07-09 <150> DE 19932922.2 <151> 1999-07-14 <150> DE 19932926.5 <151> 1999-07-14 <150> DE 19932928. 1 <151> 1999-07-14 <150> DE 19933004.2 <151> 1999-07-14 <150> DE 19933005.0 <151> 1999-07-14 <150> DE 19933006.9 <151> 1999-07-14 <150> DE 19940764.9 <151> 1999-08-27 <150> DE 19940765.7 Page 2 seql i st cor r ect ed. t xt <151> 1999-08-27 <150> DE 19940766.5 <151> 1999-08-27 <150> DE 19940832.7 <151> 1999-08-27 <150> DE 19941378.9 <151> 1999-08-31 <150> DE 19941379.7 <151> 1999-08-31 <150> DE 19941380.0 <151> 1999-08-31 <150> DE 19941394.0 <151> 1999-08-31 <150> DE 19941396.7 <151> 1999-08-31 <150> DE 19942076.9 <151> 1999-09-03 <150> DE 19942077.7 <151> 1999-09-03 <150> DE 19942079.3 <151> 1999-09-03 <150> DE 19942086.6 <151> 1999-09-03 <150> DE 19942087.4 <151> 1999-09-03 <150> DE 19942088.2 <151> 1999-09-03 <150> DE 19942095.5 <151> 1999-09-03 <150> DE 19942124.2 <151> 1999-09-03 <150> DE 19942129.3 <151> 1999-09-03 <160> 1158 <210> 1 <211> 948 <212> DNA <213> Cor ynebact er i um gl ut arri cum <220> <221> CDS <222> (101)..(925) <223> RXA02229 <400> 1 gctggttcaa cagagaccac cgcgtgtcct gggtcgacgc ctctggcgat cccaccgcac aagccttgga gattttgggt ctacaatagc gagggtgaat ttg acc atc ccc ttt 115 Leu Thr lie Pro Phe 1 Page 3 seql i st cor r ect ed. t xt gcc Al a gat Asp cgc Ar g gca Al a t ac Tyr cgc Ar g agc Ser cag G n gac Asp ggc Gy 150 ggc Gy ttt Phe gaa Gu ggc G y gct Al a 230 cca Pr o acc Thr ggc Gy cgc Ar g gcc Al a gt a Val aac Asn ttc Phe gat Asp gat Asp 120 acg Thr ggc Gy agt Ser cag G n gaa G u 200 acc Thr gct Al a ggg G y cca Pr o gcc acc gaa aac gac Ala Thr G u Asn Asp gat tta act cca gaa Asp Leu Thr Pro G u at c ggt gct gat ggt lie G y Al a Asp G y 45 ggc tcc acg gtc gac G y Ser Thr Val Asp gat gga tct ttg gct Asp G y Ser Leu Ala 75 cac tgg ctg tac tcc His Trp Leu Tyr Ser ggt acc cgc gcc ggt G y Thr Arg Ala G y 110 cat tct gcg cag gt c His Ser Ala G n Val 125 tta tcc acc tgc gac Leu Ser Thr Cys Asp 140 gat atg ggt aac cca Asp IVet y Asn Pro 155 tcg gct ctt gcc gat Ser Al a Leu Ala Asp 170 ttc ttc ccc cac ggt Phe Phe Pro His y 190 gac gca gta tcg atg Asp Al a Val Ser IVet 205 tcc tgt ggc acg gga Ser Cys G y Thr G y 220 gct gga ttg gga gaa Al a G y Leu G y G u 235 gt a gaa gtc cag at c Val u Val Gn lle 250 gcc atc atc gca ct c Ala lie i e Ala Leu 270 ttc atc atc atc ccc Phe 15 at g Met at c SIle cca Pr o gaa G u cgc Ar g 95 gt c Val cgc Ar g at c SIle cac Hi s at g Met 175 gt g Val cgc Ar g acc Thr ggc G y ttt Phe 255 ggt Gy Ile lie Ile gt g Val ct c Leu tcg Ser at g Met ggt G y cgc Ar g gtt Val aac Asn ct a Leu 160 gaa G u aac Asn gt g Val gtt Val aca Thr 240 gac Asp gag G u gt c Val cgc Ar g ct g Leu tgc Cys ctt Leu cac Hi s gat Asp ggc Gy 145 gcg Al a ct g Leu gt a Val tgg Tr p gct Al a 225 gct Al a gac Asp gt g Val acg Thr gt g Val tgg Tr p ggc G y gtt Val gtt Val at g Met 130 caa G n tgc Cys cgc Ar g gaa G u gaa G u 210 gca Al a aaa Lys ggc G y cag G n Pr o ct g Leu gtt Val ttc Phe aat Asn gat Asp gat Asp 115 ggc Gy gt a Val gtt Val gca Al a at c SIle 195 cgc Ar g gcg Al a gt g Val t cc Ser at c SIe 275 gat Asp tgt Cys aaa Lys at g Met ggt G y aat Asn 100 att SIe at c SIe ttc Phe gtg Val cct Pr o 180 gtc Val gga G y tgt Cys tgc Cys aca Thr 260 Page 4 seqI i st car r ect ed. t xt t aagat tcgc gat tgt agt t cgg <210O> 2 <21 1> 275 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 2 Leu Thr li1e I Ie Val Thr Ar g Val Leu Trp CysG y Leu Val Hi s Val As p Ib t 130 0 y 0n 145 A a Cys Leu Arg Val 0 u Tr p 0u 210 A a A a 225 A a Lys As p 0y Val 0On Ilie Pro Phe Ala Pr o Le u ValI Ph e As n As p As p 115 G31y ValI ValI A a I Ie 1 95 Ar g A a ValI Ser I Ie 275 O31u As p A a As p ValI Th r Le u Pr o A a Pr o 1 65 Th r Th r ValI A a ValI 245 Le u As p Ar g A a Ty r 70 Ar g Ser O31n As p G31y 150 G31y Ph e O31u G31y A a 230 Pr o Th r Ly s A a Ar g As p 55 Ar g Le u Ph e A a ValI 135 Le u Le u As p Le u O31u 215 Le u Ar g G31y G31y Ar g A a 40 ValI As n Ph e As p As p 120 Th r G31y Ser O31n O31u 200 Th r A a G31y Pr o Hi s Le u G31y O31u A a A a I Ie 1 O31n G31y ValI A a O31u 1 As p Ar g As p O31u Ser 265 A a 10 As p I Ie G31y As p Hi s 90 G31y Hi s Le u As p Ser 1 70 Ph e As p Ser A a ValI 250 A a Th r Le u G31y Ser G31y Tr p Th r Ser Ser vb t 1 A a Ph e A a Cys G31y 235 O31u I Ie O31u Th r A a Th r Ser Le u Ar g A a Th r 140 G31y Le u Pr a ValI G31y 220 Le u ValI I Ie As n Pr a As p ValI Le u Tyr A a O31n 125 Cys As n A a Hi s Ser 205 Thr G31y O31n A a Ph e vb t I Ie Pr a O31u Ar g ValI Ar g I Ie Hi s vb t 1 ValI Ar g Thr O31y Ph e 255 O31y <210O> 3 <21 1> 1491 <212> DNA Page seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1468) <223> RXS02970 <400> 3 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag Leu Ala Leu Lys t ac Tyr aaa Lys cac Hi s gcc Al a at g Met tta Leu ccg Pr o tcg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr t ca Ser cac Hi s aac Asn gag G u tgg Tr p ggt Gy t cc Ser gag G u ttc Phe gcc Al a 120 gcc Al a aaa Lys at g Met cca Pr o ttt Phe 200 gaa G u gac Asp aaa Lys gcg Al a acg Thr ctt Leu at c SIle aat Asn ggc Gy gag G u ct g Leu ct c Leu 170 at c SIle acc Thr gt c Val gaa G u gca Al a gac Asp t ac Tyr tcg Ser cgc Ar g gt g Val ttc Phe t cc Ser 140 gca Al a ggc Gy cac Hi s caa G n gcg Al a ttc Phe ttc Phe aaa Lys 45 gac Asp gca Al a caa G n cgc Ar g t cc Ser 125 at c SIle t ac Tyr gaa G u ttc Phe gaa G u 205 ttt Phe at c SIle gac Asp at c SIle ttc Phe aac Asn gca Al a tct Ser 110 cac Hi s cgc Ar g cgc Ar g cac Hi s tgg Tr p 190 gaa G u gaa G u gaa G u 15 aac Asn agc Ser gac Asp tta Leu gcc Al a 95 gat Asp gt g Val at g Met agc Ser cgc Ar g 175 gca Al a gaa G u ggt Gy ttc Phe gat Asp ccc Pr o ggc Gy ggc Gy 80 cgg Ar g gtt Val ttt Phe gct Al a t ac Tyr 160 cgc Ar g cca Pr o tgc Cys gct Al a 1 gga Gy cgc Ar g aaa Lys aac Asn cac Hi s ttg Leu gct Al a ttc Phe cgc Ar g 145 cac Hi s ct g Leu ttc Phe gaa G u ggc Gy tct Ser gcg Al a gt a Val gcc Al a aac Asn acc Thr gca Al a acc Thr 130 ct g Leu ggc Gy ggc Gy ct g Leu cgc Ar g 210 at g Met gt g Val cac Hi s tgg Tr p ttc Phe aac Asn aac Asn aag Lys 115 aac Asn cac Hi s gca Al a aac Asn cac Hi s 195 gca Al a at c SIle caa G n gtt Val gca Al a at c SIle cct Pr o at c SIle 100 at c SIle ggc Gy acc Thr acc Thr ccc Pr o 180 cac Hi s ct c Leu gca Al a ggt Gy gca Al a ttc Phe gct Al a gac Asp cga Ar g aac Asn gtg Val ggc Gy gga Gy gga Gy 165 acc Thr t cc Ser aag Lys gcg Al a Page 6 seql i st cor r ect ed. t xt 215 220 225 atc gtc ctg gag cca gtg gtg gga tca tca gga atc atc ctg cca cca 835 lie Val Leu G u Pro Val Val y Ser Ser Gy lie lie Leu Pro Pro 230 235 240 245 gca ggt tac tta aat ggc gtg cgc gaa ctt tgc aac aag cac ggc atc 883 Ala G y Tyr Leu Asn G y Val Arg G u Leu Cys Asn Lys His G y lie 250 255 260 ctc ttc atc gcc gac gaa gtc atg gtc gga ttc gga cgc acc gga aaa 931 Leu Phe Ile Ala Asp G u Val IVet Val y Phe y Arg Thr y Lys 265 270 275 ctg ttt gct tac gag cat gct ggc gac gat ttc cag cca gac atg atc 979 Leu Phe Ala Tyr G u His Ala G y Asp Asp Phe G n Pro Asp IVet lie 280 285 290 acc ttc gcc aag ggt gtt aac gca ggt tac gcc cca ctc ggt ggc atc 1027 Thr Phe Ala Lys y Val Asn Ala y Tyr Ala Pro Leu Gy Gy lie 295 300 305 gtg atg acc caa tca atc cgc gat acc ttc gga tca gag gca tac tcc 1075 Val IVet Thr G n Ser I e Arg Asp Thr Phe y Ser G u Ala Tyr Ser 310 315 320 325 ggc gga ctc acc tac tcc gga cac cca ctt gca gta gca ccc gcc aag 1123 G y G y Leu Thr Tyr Ser G y His Pro Leu Ala Val Ala Pro Ala Lys 330 335 340 gca gcg ctg gag att tac gcg gaa gga gag atc att cca cgc gta gct 1171 Al a Ala Leu Gu Ile Tyr Al a G u G y u Ile Ile Pro Arg Val Ala 345 350 355 cga ctt ggc gct gaa ctg atc gaa cct cgc ctt cgt gaa cta gcg gaa 1219 Arg Leu y Ala G u Leu lie G u Pro Arg Leu Arg G u Leu Ala G u 360 365 370 gaa aac gta gcg atc gct gac gtg cgg ggc atc gga ttc ttc tgg gca 1267 Su Asn Val Ala Ile Ala Asp Val Arg y Ile y Phe Phe Trp Al a 375 380 385 gtg gag ttc aat gca gac gcc act gcc atg gct gcc ggt gct gca gaa 1315 Val G u Phe Asn Ala Asp Ala Thr Al a Iet Al a Al a Gy Ala Ala G u 390 395 400 405 ttc aag gaa cgc ggc gtg tgg ccg atg atc tcc ggc aac cga ttc cac 1363 Phe Lys G u Arg G y Val Trp Pro IVet lie Ser G y Asn Arg Phe His 410 415 420 atc gcg ccg ccg ctg acc acc act gat gac gaa ttg gta gca ctg ctg 1411 lie Ala Pro Pro Leu Thr Thr Thr Asp Asp G u Leu Val Ala Leu Leu 425 430 435 gac gcg gtg gaa gct gca gcc caa gct gtc gag ctg acc ttc gct ggg 1459 Asp Ala Val G u Ala Al a Ala G n Ala Val G u Leu Thr Phe Ala y 440 445 450 gcg ttg ttc taagttttct agataacaag gcc 1491 Ala Leu Phe 455 <210> 4 <211> 456 <212> PRT <213> Cor ynebact eri um gl ut am cum Page 7 seqI i st car r ect ed. t xt <400> 4 Leu Al a Leu Lys G31y Ar g Ly s As n Hi s Le u Al a Ph e Ar g 145 Hi s Le u Ph e O31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI ValI Hi s Tr p Ph e As n As n Ly s 115 As n Hi s Al a As n Hi s 1 95 Al a I Ie Le u Hi s Thr 275 As p G31y Al a Pr o G31y Al a Ph e Al a As p Ar g As n ValI G y G31y G31y 1 65 Thr Ser Ly s Al a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s Tyr Thr Asn Phe 03 y 03 u Phe I I e 03 u Phe Ly s Hi s Al a vb t 70 Le u Pr o Ser Al a Ar g 150 Ser Thr Ser Hi s I Ie 230 Al a Le u Le u Thr ValI 310 G31y Al a O31u Ser O31u G31y ValI Al a vb t As p 135 As n Al a As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y Al a O31u Tr p 40 G31y Ser O31u Ph e Al a 120 Al a Ly s vb t Pr a Ph e 200 O31u Le u Tyr I Ie Al a 280 Al a Thr Le u Le u O31u Ser Ser O31n Al a O31y 1 Ar g le le vb t As p 1 Al a As p O31u Le u Al a 265 Tyr Ly s O31n Thr O31u 345 Ar g O31n Le u ValI 75 O31n As p O31u Hi s Ser 1 55 Thr Tyr Thr I Ie ValI 235 O31y O31u Hi s ValI I Ie 315 Ser Tyr Al a As p Tyr Ser Ar g ValI Ph e Ser 140 Al a O31y Hi s O31n Al a 220 ValI ValI ValI Al a As n 300 Ar g O31y Al a Ph e Ly s As p Al a O31n Ar g Ser 125 li1e Tyr O31u Ph e O31u 205 Ph e O31y Ar g vb t O31y 285 Al a As p Hi s (31u As n Ser As p Le u Al a As p ValI vb t Ser Ar g 1 Al a O31u O31y Ser Le u 255 O31y As p Tyr Ph e Le u 335 (31u As p Pr a O31y O31y Ar g ValI Ph e Al a Tyr 1 Ar g Pr a cys Al a O31y 240 cys Ph e Ph e Al a O31y 320 Al a I Ie Ilie Pro Arg Val Ala Arg Leu Oy Ala Ou Leu Ilie Ou Pro Arg Leu Page 8 seqI i st car r ec 355 360 Ar g (1u Leu Al a (1u 1u Asn Val Ala IleAla 370 375 G31y Phe Phe Tr p Al a Val (31u Phe Asn Al a As p 385 390 395 Ala G31y Al a Al a (31u Phe Lys O31u Ar g G31y Val 405 410 G31y Asn Ar g Phe Hi s Ilie Al a Pr o Pr o Leu Thr 420 425 Leu Val Al a Leu Leu Asp Al a Val (31u Al a Al a 435 440 Leu Thr Phe Ala G31y Al a Leu Phe 450 455 <210> <211> 1330 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (1330) <223> FRXA01009 <400> aaccgacaaa acagccgtt c acgt gct aaa gcagct cggc ted. t xt 365 Asp Val 380 Al a Thr Tr p Pr o Thr Thr Al a 03 n 445 Ar g G31y I Ie Al a N/t Al a 400 I'b t I Ie Ser 415 As p As p (31u 430 Al a Val (31u t tgat ct agg gt gaggt gag t tat t taaag act tcat aat att t tgggga gt gaact ggt t tg t ac Tyr aaa c ac Hi s gcc Al a at g vb t tt a Le u ccg Pr o aac As n gag (31u t gg Tr p ggt G31y t cc Ser gag (31u tt c Ph e g ac As p aaa Lys5 gcg Al a ac g Thr ct t Le u at c I Ie aat As n g aa (31u gca Al a g ac As p t ac Tyr t cg Ser cgc Ar g gt g ValI tt c Ph e tt c Ph e aaa Lys5 45 g ac As p gca Al a c aa O31n cgc Ar g at c I Ie g ac As p at c I Ie tt c Ph e aac As n gca Al a t ct Ser 110 g aa G31u 15 aac As n ag c Ser g ac As p tt a Le u gcc Al a 95 g at As p Le u 1 ttc gga Phe 03 y gat cgc Asp Arg ccc aaa Pr o Lys ggc aac 31y As n ggc cac (3 y His 80 cgg tt g Arg Leu gt t gct Val Al a t tt t tc gca Al a t ct Ser gcg Al a gt a ValI gcc Al a aac As n ac c Thr gca Al a ac c ttg aag ggt Leu Lys 03 y gt g caa gca Val O31n Al a cac gt t tt c Hi s Val Phe t gg gca gct Tr p Al a Al a ttc atc gac Phe Il e Asp aac cct cga Asn Pr o Ar g aac at c aac Asn Il e Asn 1 00 aag at c gt g Lys IlIe Val 115 aac ggc ggc Asn (1y (1y t cg at g gcc cgt ggc gaa t tc t cc cac gt g Ser I'bt Ala Arg Gy Gu Phe Ser His Val Phe Phe Thr Page 9 seql i st cor r ect ed. t xt 120 125 130 gcc gac gcc atc gag cac tcc atc cgc atg gct cgc ctg cac acc gga 547 Ala Asp Ala lie G u His Ser lie Arg IVet Ala Arg Leu His Thr G y 135 140 145 cgc aac aaa att ctg tcc gca tac cgc agc tac cac ggc gca acc gga 595 Arg Asn Lys IIe Leu Ser Ala Tyr Arg Ser Tyr His G y Ala Thr G y 150 155 160 165 tcc gcg atg atg ctc acc ggc gaa cac cgc cgc ctg ggc aac ccc acc 643 Ser Ala Met Met Leu Thr G y G u His Arg Arg Leu G y Asn Pro Thr 170 175 180 acc gac cca gat atc tac cac ttc tgg gca cca ttc ctg cac cac tcc 691 Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro Phe Leu His His Ser 185 190 195 tca ttc ttt gcc acc acc caa gaa gaa gaa tgc gaa cgc gca ctc aag 739 Ser Phe Phe Ala Thr Thr n G u G u G u Cys G u Arg Ala Leu Lys 200 205 210 cac ttg gaa gat gtc atc gcg ttt gaa ggt gct ggc atg atc gca gcg 787 His Leu Gu Asp Val Ile Ala Phe G u Gy Al a G y Mt Ie Ala Al a 215 220 225 atc gtc ctg gag cca gtg gtg gga tca tca gga atc atc ctg cca cca 835 lie Val Leu G u Pro Val Val y Ser Ser Gy lie lie Leu Pro Pro 230 235 240 245 gca ggt tac tta aat ggc gtg cgc gaa ctt tgc aac aag cac ggc atc 883 Ala G y Tyr Leu Asn G y Val Arg G u Leu Cys Asn Lys His G y lie 250 255 260 ctc ttc atc gcc gac gaa gtc atg gtc gga ttc gga cgc acc gga aaa 931 Leu Phe lie Ala Asp G u Val IVet Val y Phe y Arg Thr y Lys 265 270 275 ctg ttt gct tac gag cat gct ggc gac gat ttc cag cca gac atg atc 979 Leu Phe Ala Tyr G u His Ala G y Asp Asp Phe G n Pro Asp IVet IIe 280 285 290 acc ttc gcc aag ggt gtt aac gca ggt tac gcc cca ctc ggt ggc atc 1027 Thr Phe Ala Lys y Val Asn Ala y Tyr Ala Pro Leu Gy Gy lie 295 300 305 gtg atg acc caa tca atc cgc gat acc ttc gga tca gag gca tac tcc 1075 Val IVet Thr G n Ser I e Arg Asp Thr Phe y Ser G u Ala Tyr Ser 310 315 320 325 ggc gga ctc acc tac tcc gga cac cca ctt gca gta gca ccc gcc aag 1123 G y G y Leu Thr Tyr Ser G y His Pro Leu Ala Val Ala Pro Ala Lys 330 335 340 gca gcg ctg gag att tac gcg gaa gga gag atc att cca cgc gta gct 1171 Ala Ala Leu u Ile Tyr Ala u G y u Ile Ile Pro Arg Val Ala 345 350 355 cga ctt ggc gct gaa ctg atc gaa cct cgc ctt cgt gaa cta gcg gaa 1219 Arg Leu Gy Ala G u Leu lie G u Pro Arg Leu Arg G u Leu Ala G u 360 365 370 gaa aac gta gcg atc gct gac gtg cgg ggc atc gga ttc ttc tgg gca 1267 Su Asn Val Ala Ile Ala Asp Val Arg y Ile y Phe Phe Trp Al a 375 380 385 gtg gag ttc aat gca gac gcc act gcc atg gct gcc ggt gct gca gaa 1315 Val G u Phe Asn Ala Asp Ala Thr Ala IVet Ala Ala G y Ala Ala G u Page seqI i st car r ect ed. t xt 400 390 ttc aag gaa cgc ggc Phe Lys Ou Arg Gy 410 1330 <210O> 6 <211> 410 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 6 Le u G31y Ar g Ly s As n Hi s Le u Al a Ph e Ar g 145 Hi s Le u Ph e O31u G31y 225 li1e As n G31y Al a Leu Lys Ser Val O31n Al a Hi s Val Val Tr p Al a Al a Phe Il e Asn Asn Pro Thr Asn Il e 1 00 Al a Lys IlIe 115 Thr Asn G31y 130 Leu Hi s Thr O3 y Al a Thr O31y Asn Pr o 1 80 Leu Hi s Hi s 1 95 Ar g Al a Leu 210 I'b t Ilie Al a Ilie Leu Pr o Lys Hi s 03 y 260 Ar g Thr O31y 275 O31y Al a Ph e Al a As p Ar g As n ValI O31y O31y O31y 1 65 Thr Ser Ly s Al a Pr o 245 li1e Ly s Tyr Thr Asn Phe Asp 10 Ly s 03 u 03 u 03 u Lys Hi s Se r Tr p Ser Al a 40 Al a 03 u 03 y Se r Thr 55 [Vb t G y Ser G n Leu 70 Leu Val O31u Al a I Ie 90 Pr o Al a Phe O31y As n 1 Ser Nib t Al a Ar g O31y 120 Al a As p Al a I I e 03 u 135 Ar g As n Lys I Ie Leu 150 Ser Al a Nib t Nib t Leu 1 70 Thr As p Pr o As p I Ie 1 Ser Phe Phe Al a Thr 200 Hi s Leu 03 u As p Val 215 li1e Val Leu O31u Pr o 230 Ala O31y Ty r Leu As n 250 Leu Phe I I e Al a As p 265 Leu Phe Al a Ty r 03 u 280 G31y Ar g O31n Le u ValI 75 O31n As p O31u Hi s Ser 1 55 Thr Tyr Thr I Ie ValI 235 G31y O31u Hi s O31u Al a As p Tyr Ser Ar g ValI Ph e Ser 140 Al a G31y Hi s O31n Al a 220 ValI ValI ValI Al a Ph e Ph e Lys5 As p Al a O31n Ar g Ser 125 li1e Tyr O31u Ph e O31u 205 Ph e O31y Ar g vb t O31y 285 O31u As n Ser As p Le u Al a As p ValI vb t Ser Ar g 1 Al a O31u O31y Ser Le u 255 O31y As p Ph e As p Pr o O31y G y Ar g ValI Ph e Al a Tyr 1 Ar g Pr o cys Al a O31y 240 Cys Ph e Ph e O31n Pro Asp I'bt I Ie Thr Phe Al a Lys O31y Val Page 11 Asn Al a 0 y Tyr Al a Pr o 305 Ser ValI I Ie Ar g G31y 385 Al a G31y Al a Pr o Ar g 355 Le u Ph e Al a I Ie Ser 325 Ly s Al a G31u Al a G31u 405 ValI 310 G31y Al a Ar g G31u ValI 390 Ph e 295 G31y Al a Le u As n 375 G31u Ly s seql i st car r ect ed. t xt 300 Thr O31n Ser I Ie Ar g Asp 315 Leu Thr Ty r Ser 03 y Hi s 330 Leu G3 u I I e Ty r Al a G3 u 345 a3 y Al a a3 u Leu I I e G3 360 365 Val Al a I Ie Al a Asp Val 380 Phe As n Al a As p Al a Thr 395 (31u Ar g a31y 410 Ph e Le u 335 O31u Ar g a31y vb t <210O> 7 <21 1> 792 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (769) <223> RXC02390 <400> 7 gct ggt ggt g ct gacccat a cgct ggaact ccaact gct g t tgat accgc caagat gt tt ggccgcgagg at ct cgt agc t cgct tcgag t cat aggccg gt g gag t gg acc gct Val Ou Trp Thr Ala tt t Ph e cct Pr o gcg Al a ac g Thr at t I Ie ggg a31y c ag (31n ac c Thr ac c Thr gct Al a ac g Thr gga a31y aag Ly s cgt Ar g at t lIe tt c Ph e gt c ValI gt g ValI at c I Ie ct g Le u aac As n ct g Le u ct c Le u gcc Al a gga a31y cag O31n 75 cgc Ar g gcc Al a ct c Le u ct c Le u ggc a31y gca Al a ct c Le u t cg Ser g at As p aat As n cgc Ar g at c I Ie 45 gcg Al a gt c ValI gcg Al a gcc Al a tt g Le u tt a Le u gt c ValI gcg Al a ggc a31y t cg Ser cga Ar g 110 gt g ValI 15 gcc Al a ac c Thr ct g Le u ggc a31y ag a Ar g 95 cct Pr o ggc a31y ac c Thr gga a31y ct c Le u ac g Thr gag O31u at c I Ie tt a Le u t cc Ser ac g Thr act Thr ct a Le u at c I Ie ccg Pr a gcg Al a t gg Tr p ccg Pr a tt c Ph e gcc Al a 1 00 gat gcg gt a gaa Asp Ala Val COu 115 Page 12 gca ct g gga acc cgc act cag gt a t Al a Leu O31y Thr Ar g Thr O31n Val T 120 125 ct g t ca aac cct aaa gt t gt c at g t Leu Ser Asn Pr o Lys Val Val I'b t T 135 140 ttg atg cca gcg cac cca tca ccg g Leu I'b t Pr o Al a Hi s Pr o Ser Pr o\ 150 155 gcg at t t ta gt g cag acc t tt gt t a Ala Ilie Leu Val O31n Thr Phe Val T 1 70 gt c t ct acg gag cgt gt g cgc aaa g Val Ser Thr O31u Ar g Val Ar g Lys P 185 1 t tt gac ct g ct t gct ggc gt t gt c t Phe Asp Leu Leu Al a G31y Val Val F 200 205 ct g t at gaa ggc ct g acc ggt t ta c Leu Tyr 03 u 03 y Leu Thr 03 y Leu L 215 220 t cc <210> 8 <211> 223 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn Iist cor r ect ed. t xt at cga caa ggt t tg gcc acc aac yr Arg O31n G31y Leu Al a Th r Asn 130 ac t tc gcg gca at t ct g gct ccg yr Phe Al a Al a I Ie Leu Al a Pr o 145 itg ct g gcg t tc t ct at c at c gt g (al Leu Al a Phe Ser Ilie Ilie Val 160 165 cc t tc t ct gct gt g t gc ct c at t hr Phe Ser Al a Val Cys Leu Ilie 175 180 ica at g ct g cgt gca ggt ccc t gg I a I'b t Leu Ar g Al a G31y Pr o Tr p 90 195 t c ct c gt t gt g ggt gt g act ct g The Leu Val Val 03 y Val Thr Leu 210 t c ggg t aaaggcat a aaaaat ggct .eu 03 y <400> 8 Val O31u Ser Leu Ar g Ser Leu Thr Thr Thr Tyr Leu Leu I I e Pr o As p O3 y Leu 130 Al a I I e 145 Tr p Thr Al a Phe G31y Thr Leu I Ie Leu Leu Asn Leu Val G31y Ser Ar g ValI Tyr Ser As p Al a 115 Al a Le u G31y Hi s ValI Ser lIe Ar g (31u As n Pr o Pr o Al a Thr I Ie 70 G31y O31n Al a Le u Le u 150 As p I Ie Le u 55 Le u Tyr Ph e Le u Ser 135 vb t Thr Al a 40 Thr O31y Lys5 Ar g O31y 120 As n Pr o Ph e O31y ValI I Ie Le u Ph e 1 05 Thr Pr o Al a 10 Phe Leu Leu Val Al a O31y Val O31y Al a Il e 03 n Leu Leu Arg Ser 90 Asn Al a Asp Ar g Thr O31n Lys Val Val 140 Hi s Pr o Ser 1 Page 13 Ar g I Ie Al a ValI Al a Al a ValI 125 vb t Pr o Le u ValI Al a O31y Ser Ar g 110 Tyr Tyr ValI Al a Thr Le u O31y Ar g Pr o Ar g Ph e Le u seqI i st car r ect ed. t xt Phe Ser Ilie lieVal Ala Ilie Leu Val O31n Thr Phe Val Thr Phe Ser 165 170 175 Al a Val Cys Leu I Ie Val Ser Thr O31u Ar g Val Ar g Lys Al a I'b t Leu 180 185 190 Ar g Al a O31y Pr o Tr p Phe As p Leu Leu Al a O31y Val Val Phe Leu Val 195 200 205 Va 1O31y Val Thr Leu Leu Ty r O31u O31y Leu Thr O31y Leu Leu O31y 210 215 220 <210O> 9 <21 1> 897 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> 101) 874) <223> RXCO1796 <400> 9 at gtaact cg at caggt gga aat gcccgca aaagt ggcgg cggt ggccga gggat ggccg t t ggt gcggc at cggt ggcc t gct act agt cgggct ct t c t tg ct c ct t ggc Leu Leu Leu 03 y aac As n gag O31u O31y tt c Ph e at c I Ie ac c Thr t ca Ser c ag O31n c ac Hi s gcc Al a gga O31y g at As p gt c ValI t ac Tyr t gc Cys c aa O31n ggt G31y 120 t ac Tyr at c lIe t cc Ser aac As n g aa (31u gag (31u tt c Ph e gct Al a g aa (31u c ac Hi s c ag O31n gga O31y agt Ser t gg Tr p ac a Thr t ct Ser tt t Ph e gcc Al a gt c ValI 140 gt t ValI gcc Al a g at As p 45 c ag O31n tt g Le u gcg Al a g ac As p ccg Pr a 125 c aa O31n tt a Le u ggc O31y g at As p act Thr act Thr t cc Ser tt g Le u 110 ct t Le u aac As n ggc O31y tt t Ph e cgc Ar g ct t Le u tt c Ph e ggg O31y tt c Ph e c ag O31n gag O31u 1 g at As p g at As p ct t Le u cca Pr a aaa Ly s ccg Pr a tt c Ph e at g O31y 145 c aa O31n c ac Hi s t ac Tyr gct Al a aac As n tt t Ph e g at As p t ac Tyr 130 ac a Thr c ag O31n c aa O31n ac c Thr gct Al a ac c Thr t gt Cys 1 00 at g vb t gt g ValI gga O31y t cc aat t ac aac gat ccg ggc gct gat t cc Se r As n Ty r As n As p Pr o O31y Al a As p Se r aac gcc gt c aag at c gag Asn Ala Val Lys Ilie Ou Page 14 seql i st cor r ect ed. t xt 150 155 160 ttg cag gcc gat tgc tac gca ggc att tgg gct aat cac Leu G n Al a Asp Cys Tyr Al a G y lie Trp Al a Asn Hi s 170 175 ggc ccg gat ccg cta ctc caa ccc atc acc gaa tct gag Sy Pro Asp Pro Leu Leu n Pro lie Thr u Ser u 185 190 gct ctc ctt gct gca agc gcc gtg ggc gac gac aat atc Ala Leu Leu Ala Ala Ser Ala Val y Asp Asp Asn Ile 200 205 210 t cc ggt ggc gat gtc aat cct gaa agc tgg act cac ggc Ser G y G y Asp Val Asn Pro G u Ser Trp Thr His G y 215 220 225 cag cgc aaa gac gcg ttc ctc gcc ggc tac aac acc ggc G n Arg Lys Asp Ala Phe Leu Ala y Tyr Asn Thr y 230 235 240 gcc tgc gac ttc ctc ggc cgg ggc gtc tac aac gac gct Ala Cys Asp Phe Leu G y Arg y Val Tyr Asn Asp Ala 250 255 cttttcgacg tct <210> <211> 258 <212> PRT <213> Cor ynebact eri um gl ut arri cum 165 t cc agc gaa Ser Ser G u 180 ct a gat t cc Leu Asp Ser 195 cag caa cga Sn G n Arg t ca t cg cag Ser Ser G n cag atg agc G n bet Ser 245 t aaagcat t g <400> Leu 1 Asp Asp Leu Pr o Lys Pr o Phe Mbet Sy 145 Leu Leu G y G n Thr G n His Cys G n Tyr Tyr Thr Ala G n Ala Asn Ser Thr Phe Tyr Cys 100 Asp n IVet 115 Tyr Ile Val 130 Thr Leu G y Gy 5 I e Thr Ser Sy G n Pr o Ar g Al a Leu Asn Pro Su Ser G y Al a Phe Ser Ile G u 70 Thr y Ser Asp G n Phe Hi s G u 135 Ser Asn 150 Al a Sy Asp 40 Val Tyr Cys G n Sy 120 Tyr Tyr G u G u Al a Asn Thr Sy Asp 105 Al a Sy Asn Asp Thr Al a bet Pr o 75 Al a Tyr Asn Hi s Pr o 155 Val Al a Asp G n Leu Al a Asp Pr o 125 G n Al a G y G y Asp Phe Cys Arg Leu Leu Leu Phe Thr G y Thr Phe Al a n Leu G u Ser Asn 160 Trp Al a 175 Ala Val Lys Ile u Leu n Ala Asp 165 Tyr Ala G y Ile Page seqi i st car r ect ed. t xt As n Hi s Se r Ser Ou Gy Pro Asp Pro Leu Leu O1n Pr o I Ie Th r O31u 180 185 190 Ser O31u Leu As p Ser Al a Leu Leu Al a Al a Se r Al a Val G31y Asp Asp 195 200 205 As n IIe O31n O31n Ar g Ser G31y G31y As p Val As n Pr o O31u Ser Tr p Thr 210 215 220 His O31y Ser Ser O31n O31n Ar g Lys As p Al a Phe Leu Al a O31y Ty r As n 225 230 235 240 Thr O31y O31n N/bt Ser Al a Cys Asp Phe Leu O31y Ar g O31y Val Tyr Asn 245 250 255 Asp Al a <210O> 11 <21 1> 771 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (748) <223> RXCO1207 <400> 11 ct tcat gat c t caccggcag agcgcgt t tt gt tacagcgc at tt t tgaac aat ccgt aca ccaact tcag gagaaaaaca gt aaact gt g act t tgaaaa gt g agc aga at Val Ser Arg II c t at e Tyr g ac As p g at As p t at Tyr ct g Le u ggC O31y gca Al a gt t ValI ac c Th r gcc Al a gt c ValI ct c Le u ac c Th r t gg Tr p gcg Al a C ag O31n at g vb t 120 c aa O31n gcc Al a t gc Cys c ac Hi s ac c Th r gt g ValI cca Pr o cgc Ar g t cc Ser c ag O31n gct Al a ggc O31y c aa O31n gcg Al a ct t Le u cca Pr o cgt Ar g ct c Le u tt c Ph e 45 CCC Pr o gga O31y tt c Ph e ccg Pr a ct g Le u 125 gca Al a gt t ValI aac As n g at As p ct t Le u t gg Tr p t gg Tr p 110 c ac Hi s gca Al a 15 gt c ValI aac As n at g vb t gt g ValI cct Pr a 95 aac As n cca Pr a ggC O31y ct t Le u gag O31u CCC Pr a c ac Hi s ggt O31y ct t Le u gt t ValI 1 ct a Le u CCC Pr a gca Al a gt t ValI t cc Ser gga O31y ggC O31y gcc Al a aag Lys5 ac g Th r gt a ValI cca Pr a t at Ty r ct g Le u g at As p at t I Ie 130 gcg Al a g at As p gcc Al a gt g ValI t ct Ser t cc Ser ac c Th r 115 g aa O31u gct Al a ac c Th r aac As n ct c Le u gcg Al a at c I Ie 1 00 cgt Ar g tt g Le u Page 16 seqI i st car r ect ed. t xt cgc caa acc gga cca atg gct gtc tcc tcc gcc aac atc Ar g O31n Thr G31y Pro I'b t Al a Val Ser Ser Al a Asn I Ie 135 140 145 act cct cca acc acc gt g ct g gag gct cgt cag cag ct C Thr Pr o Pr o Thr Thr Val Leu (31u Al a Ar g 31n O31n Leu 150 155 160 gt c gct gt c t ac ct c gat ggt ggc gaa t gc gcg ct g gcc Val Al a Val Tyr Leu Asp G31y 31y O31u Cys Al a Leu Al a 170 175 acc at c gt g gat at t t ca ggc ccc gca cca aag at t tt g Thr Ilie Val Asp Ilie Ser G31y Pr o Ala Pr o Lys Ilie Leu 185 190 gcc atc agc gca gaa cgc gtt ggc gaa gta ctt gga gtg Ala Ilie Ser Al a O31u Ar g Val G31y O31u Val Leu G31y Val 200 205 210 agc ct g cgc t aaat gggag t cggt t tcgc ggg Ser Leu Ar g 215 <210> 12 <211> 216 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn gga G31y c aa O31n cct Pr o 1 gag O31u gca Al a cat Hi s aat As n 1 t ca Ser ggt G31y g aa (31u <400> 12 Val Ser Leu Lys Pr o Thr Al a Val Val Pr o Ser Ty r 03 y Leu 31y As p Al a I I e 130 As n I I e 145 03 n Leu Leu Al a Ar g I Ie Al a Al a Asp Thr Al a As n Val Leu Ser Al a Ser I Ie 1 00 Thr Ar g 115 (3 u Leu Ser 03 y As n (1n Thr Pr o 1 Rf Ty r ValI Le u Le u ValI O31n li1e G31y Le u Hi s As n 1 65 Ser Cys Al a G31y Al a 55 Ser Ly s Pr o ValI O31n 135 Pr o Al a I Ie As p Ly s 25 G31y Ly s As p Le u Al a 1 05 Le u G31y Th r Ty r As p 1 85 O31n 10 Al a Cys Hi s Th r ValI 90 Pr a Ar g Pr a Th r Le u 1 70 li1e As p O31y As p Ar g I Ie O31u Ser vb t vb t ValI 1 As p Ser Ser O31n Al a O31y O31n Al a Le u Pr a Al a 140 Le u O31y (31y Ar g Le u Ph e Pr a O31y Ph e Pr a Le u 125 ValI O31u O31y Pr a Al a ValI As n As p Le u Tr p Tr p 110 Hi s Ser Al a O31u Al a 1 Al a ValI As n vb t ValI Pr a As n Pr a Se r Ar g Cys 1 Pr a Ile LeuArg Ou OyAlalleSer Ala(3uArgVal Oy OuVal Leu Page 17 seqi i st car r ect ed. t xt 195 200 205 G31y ValI Se r Ala (31u Se r L eu Ar g 210 215 <210O> 13 <21 1> 1026 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1003) <223> RXCO0657 <400> 13 gt gcggat cg ggt at ccgcg ct acact t ag aggt gt t aga gat cat gagt t t ccacgaac tgtaacgcag gattcaccaa tcaatgaaag gtcgaccgac at t Ar g gct A a cca Pr o at t I Ie at c I Ie at g vb t gga G31y c ac Hi s g aa (31u 1 50 gt c ValI gt c ValI gct A a ag c Ser c aa O31n g aa (31u c ac Hi s ccc Pr o t cc Ser 120 aag Ly s ag c Ser c aa O31n gca A a aat As n ac c Th r ct t Le u cgt Ar g at c I Ie gcc A a at g vb t cca Pr o t ac Ty r gca A a 1 70 gt a ValI acc Th r at g vb t t t c Ph e gac As p 75 gct A a aca Th r gt c ValI gt c ValI ggc G31y 155 acc Th r g at As p gcc A a cct Pr o g at As p at c I Ie g aa O31u at g vb t at g vb t gt t ValI 140 cca Pr a g aa O31u t cg Ser aaa Ly s tt c Ph e ct c Le u cat Hi s agt Ser gt c ValI 110 t cc Ser gt c ValI gt c ValI gca A a gcc A a ggc G31y t ac Ty r gcc A a gt a ValI at c I Ie ggt G31y t cc Ser g aa O31u ggt G31y 1 gcg A a at g vb t 1 t ca Ser at t I Ie gca A a g aa O31u gcg A a g ac As p t cc Ser ggt G31y g ac As p 145 gt g ValI g aa O31u aaa Ly s cca Pr a gag O31u gcc A a cca Pr a at g vb t cgc Ar g gca A a 130 ag c Ser g at As p gct A a agc act gaa Ser Thr 03 u c aa O31n ct t Le u gga G31y ct g Le u g aa (31u ct g Le u gga G31y 115 gt g ValI gca A a ggc G31y g aa (31u gct A a cgc Ar g at g vb t g aa (31u at c I Ie tt g Le u 1 00 ct c Le u gt c ValI gt c ValI t cc Se r gct A a 1 ggc gcc gaa ct cgt t gca gt t cac acc tgg at ggac at gcag gt acag Page 18 Gy Ala G u Leu Val Al a Val 185 gca tca ctt gca ggt ctt gca Ala Ser Leu Ala Gy Leu Ala 200 gaa cgt cag caa acc gac atg Su Arg On On Thr Asp IVet 215 220 gaa aag tac cca agt gta acc G u Lys Tyr Pro Ser Val Thr 230 235 cca gtt cgc gca ctt gca gaa Pro Val Arg Ala Leu Al a G u 250 gtt ggt tcc cat ggt cgt ggc Val G y Ser His G y Arg G y 265 acc tcc cgc gca ctg ctg caa Thr Ser Arg Ala Leu Leu G n 280 cgc cca cct gag aag att aag Arg Pro Pro G u Lys Ile Lys 295 300 <210> 14 <211> 301 <212> PRT <213> Cor ynebact er i um gl ut ar <400> 14 et Ser Thr G u Asp I e Val 1 5 Ser Lys G n Ala Val Arg Trp lie Pro Leu Arg Leu Ala Ser Al a G u Gy bet Val Pro Pro 55 G u Ala Leu G u Lys I e Asn 70 Al a Pro G u lle Lys Ile G y Asp IVet Leu Leu G u IVet Ser 100 Ser Arg y Leu y G y Leu 115 G y Ala Val Val Ser His Ala 130 135 Asp Ser Ala Val Asn G u Asp 145 150 seql i st cor r ect ed. t xt His Thr Trp Met Asp Met G n Val 190 195 gct gct caa cag cag tgg gat gaa Ala Ala Gn Gn n Trp Asp u 205 210 ctg atc gaa cgc ctc gca cca ctg Leu lie G u Arg Leu Ala Pro Leu 225 gtc aag aag atc atc acc cgt gac Val Lys Lys lie lie Thr Arg Asp 240 gca tct gaa aac gcg cag ctc cta Ala Ser G u Asn Ala G n Leu Leu 255 260 gga ttt aag ggc atg ctc ctt ggc G y Phe Lys G y IVet Leu Leu G y 270 275 t cc gca ccg tgc cca atg atg gtg Ser Ala Pro Cys Pro Vet Vet Val 285 290 aag tagtttcttt taagtttcga tgc Lys 1026 Val Val Ala Val Asp y Ser Al a Tyr G u Al a Thr Asp 105 Sy Cys Lys 10 Asn Thr Al a Thr Met Pro Leu Phe Asp Arg Asp Ile 75 Ile Ala G u 90 Al a Thr IVet vet Val Vbet Pro Val Val 140 Tyr G y Pro 155 Page 19 Asn G n Asp Al a Sy SIle Sy 125 Val Val Asp Al a Arg G y Leu Tyr G n Al a G u Val Pro Ile Vt G y Val Ser Arg G u Val G y 160 seql i st cor r ec Val Asp Gy Ser u Val Ser n n Ala Thr 165 170 Su Ala u Ala Arg Gy Ala u Leu Val Ala 180 185 Asp Iet G n Val G n Al a Ser Leu Ala y Leu 195 200 On Trp Asp Gu Val Gu Arg Gn n Thr Asp 210 215 Leu Ala Pro Leu Val G u Lys Tyr Pro Ser Val 225 230 235 Ile Thr Arg Asp Arg Pro Val Arg Ala Leu Ala 245 250 Ala G n Leu Leu Val Val G y Ser His G y Arg 260 265 IVet Leu Leu G y Ser Thr Ser Arg Ala Leu Leu 275 280 Pro IVet IVet Val Val Arg Pro Pro G u Lys Ile 290 295 <210> <211> 1059 <212> DNA <213> Cor ynebact eri um gl ut arr cum <220> <221> CDS <222> (101)..(1036) <223> RXC00552 <400> ccgccaacaa ggcagcaaag ctcgatccaa ttgacgcctt gtttttaagg tagccacaca tcgcactaga ctgaagaact t ed. t xt G u Tyr Val Hi s Al a Al a 205 IVet Leu 220 Thr Val G u Al a G y G y G n Ser 285 Lys Lys 300 Al a Thr 190 Al a SIe Lys Ser Phe 270 Al a Phe 175 Tr p G n Gu Lys G u 255 Lys Pr o at t SIle cag G n at c SIle gat Asp aac Asn aag Lys ctt Leu tgg Tr p at c SIle tgc Cys at g Met agt Ser t ac Tyr cgt Ar g act Thr gcg Al a ttt Phe aaa Lys gca Al a gag G u cac Hi s tac Tyr aag Lys 75 gt c Val ttc Phe ct c Leu ggc G y at t I e tgg Tr p cgc Ar g acc Thr tgc Cys at c I e 45 aaa Lys t cc Ser gat Asp ccg Pr o gag G u aat Asn aag Lys gag G u gag G u ct c t ct Leu Ser 15 t ca ct g Ser Leu gga acc G y Thr acc cgc Thr Arg ggt ggc G y G y 80 atc gtt Ile Val 95 Page gcgttatgag taaaagcctc gtg gct acc tca aaa 115 Val Ala Thr Ser Lys 1 gac cct aaa gcg gtt 163 Asp Pro Lys Ala Val aat ctt cgt ggc cgc 211 Asn Leu Arg y Arg gtg ggc gga gat att 259 Val y y Asp Ile gag tac cca ggt ttc 307 G u Tyr Pro y Phe gct gag gat ttc cca 355 Ala G u Asp Phe Pro gcc ttc ggc gct cca 403 Ala Phe G y Ala Pro 100 seqi i st car r ect ed. t xt g at As p ct g Le u gt t ValI tt c Ph e 1 50 gca A a gt c ValI at g vb t gt t ValI ct c Le u 230 aaa Ly s gag O31u cct Pr o t gt cys gt t ValI 310 ct c Le u cca Pr o 120 tt c Ph e g ac As p at t I Ie t ac Tyr aac As n 200 t ac Tyr gt t ValI gt c ValI t gc cys t gc cys 280 gca A a t ct Ser gt g gat gaa ValI As p (1u c ag O31n g ac As p gt t ValI t ct Ser 1 70 ac c Thr ggt G31y gag O31u g at As p c ac Hi s 250 aac As n gcc A a gct A a gt c ValI ggc O31y gt c ValI 155 gga O31y ggc G31y t t c Ph e cag O31n aag Ly s 235 t gc Cy s gaa O31u aat As n gcg A a aat As n cgc Ar g 140 cct Pr o aaa Ly s gga G31y aaa Ly s tt t Ph e 220 cgc Ar g at c I Ie g at As p gt t ValI g at As p 300 aac ggc As n (1y 110 gag ctt O3 u Leu 125 aac gca As n A a gac gt a As p Val t ac gac Ty r As p at t cgt I Ie Ar g 1 gag gt c O3 u Val 205 ggc aac O31yAs n at g cat l'b t Hi s cat t gc Hi s Cys gat t gc As p Cys 270 gag acc O3 u Thr 285 t tc gct Phe A a gt c gt c ggt ggc Val Val Gy Gy gt g ValI at g vb t g aa O31u g at As p 1 75 t gt Cys t ac Tyr aag Ly s at g vb t g at As p 255 cgc Ar g cgt Ar g gag O31u g aa O31u g aa O31u ac c Thr 1 ct c Le u gag O31u c aa O31n G31y g aa O31u 240 act Thr gag O31u cat Hi s gcc A a gcc A a 145 act Thr aaa Ly s at c I Ie at c I Ie ct g Le u 225 tt c Ph e ccc Pr o ct c Le u t gc Cys cgt Ar g 130 c ag O31n cat Hi s g ac As p ct g Le u g at As p 210 t gg Tr p ggc O31y ac c Thr gt g ValI aag Ly s 290 gt t ValI g at As p ggc O31y tt c Ph e cct Pr a 1 t ca Ser ggc O31y ggc O31y g at As p aaa Ly s 260 at g vb t g aa O31u 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 059 caa gga at t gat ccg ct c On Oy lieAsp Pro Leu t aaaaagggt at ggt ggct g ggt <210O> 16 <21 1> 312 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 16 ValI Ala Thr Ser Lys I Ie Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser 1 5 10 Asp Pr o Lys Ala Val O31n Leu Tr p O31n Ar g O31u Leu Cys O31u Ser Leu Page 21 seqI i st car r ect ed. t xt Gy Arg Ilie Leu As n ValI G31u A a A a G31y A a A a 145 Thr Ly s I Ie I Ie Le u 225 Ph e Pr o Le u cys G31y 305 Ar g G31y Pr o As p G31y G31y 115 G31y I Ie As p Ly s Ser 1 95 G31y O31u O31u As n Le u 275 Ar g As p I Ie Ph e Pr o Pr o Hi s O31u Ly s I Ie 1 65 ValI Le u I Ie Ser Tyr 245 Ph e cys Ar g Le u As p Ar g Le u O31u Ly s ValI 135 Ly s O31u Thr I Ie Ar g 215 Tyr O31u Hi s As p A a 295 Thr 25 1 1 e Ser 40 Cys Lys [Vb t (1n Ser Val Leu Lys 1 05 Pro O31n 120 Phe Phe As p A a I Ie G31u Ty r Cys 1 85 Asn Arg 200 Ty r 03 y Val Phe Val G31y Cys Leu 265 Cys Phe 280 A a I I e Ser Thr His G1y Ilie Ala Ty r I Ie Lys Phe Lys Tr p Ser 75 Lys Val Ar g Asp 90 Val As p 1u As n 31n Val As n (1u 125 Asp G31y Ar g As n 140 Val Val Pr o As p 1 55 Ser 03 y Lys Ty r 1 70 Thr 03 y 03 y I I e 03 y Phe Lys (3 u 205 03 u 03 n Phe 03 y 220 As p Lys Ar g I'b t 235 Hi s Cys I I e Hi s 250 As n 1u As pAs p Ala As n Val (31u 285 A a A a As p Phe 300 Asn G31y Th r Lys Thr Ar g (31u (1y (1y 03 u I I e Val G31y Val Val 110 Leu Val 031u A a [Vb t 03 u Val 031u Thr 1 Asp Asp Leu 1 Ar g Cys O31u 1 Val Tyr O31n Asn Lys 03 y Hi s [Vbt O31u 240 Cys Asp Thr 255 Cys Ar g O31u 270 Thr Ar g Hi s A a 03 u 03 n <210O> 17 <21 1> 1578 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1555) <223> RXN00351 <400> 17 Page 22 seql i st cor r ect ed. t xt gaaggctgct gctaagaaaa cgaccaagaa gaccactaag aaaactacta aaaagaccac cgcaaagaag accacaaaga agtcttaagc cggatcttat atg gat gat tcc aat IVet Asp Asp Ser Asn agc Ser cca Pr o ggg Gy cct Pr o gtt Val t ac Tyr at t SIle gt a Val gcc Al a ttg Leu 150 ccc Pr o at t SIle caa G n gcc Al a gca Al a 230 gt a Val ggt Gy t cc Ser act Thr ct g Leu ggc Gy act Thr ct c Leu 120 gt g Val cag G n cct Pr o cga Ar g gca Al a 200 tct Ser gt g Val gtt Val t at Tyr gtt Val gat Asp cct Pr o t ca Ser gt g Val ttc Phe gt g Val cgc Ar g cct Pr o 170 at g Met aac Asn gt g Val gag G u gct Al a agc Ser ct g Leu gtt Val gtt Val 75 aac Asn tac Ty r gct Al a cag G n cct Pr o 155 gat Asp ct g Leu tt c Phe ggt Sy at t SIle 235 aac Asn at c SIle gaa G u gca Al a gt c Val gca Al a aac Asn gaa G u gac Asp 140 gat Asp ct g Leu ggc Sy ctt Leu cag G n 220 ggc Sy cgt Ar g t cc Ser caa G n 45 ccc Pr o ct c Leu acg Thr acc Thr gcc Al a 125 t at Tyr ttg Leu ttc Phe gca Al a gcg Al a 205 ccg Pr o gct Al a ct g Leu ccc Pr o cat Hi s gaa G u act Thr ct g Leu gat Asp 110 gt g Val cag G n aag Lys cgt Ar g gat Asp 190 tta Leu gac Asp cat Hi s cca Pr o 15 agc Ser cgt Ar g cca Pr o gca Al a tgg Tr p 95 tgg Tr p agc Ser ct g Leu at c SIle cag G n 175 ttg Leu acc Thr acc Thr gtt Val gt g Val ccc Pr o gga Sy ttt Phe agt Ser 80 cct Pr o tgg Tr p caa G n ttg Leu ggt G y 160 ct g Leu gt g Val cag G n ttg Leu gaa G u 240 at g Met ggc Sy tgg Tr p aca Thr t at Tyr ttc Phe gcg Al a gcg Al a 130 gtt Val ttc Phe tgg Tr p ttc Phe gtt Val 210 gt c Val gct Al a act Thr ctt Leu gt c Val gat Asp gaa G u cac Hi s ttt Phe 115 gca Al a cct Pr o ct c Leu cgt Ar g cat Hi s 195 gcc Al a agt Ser gac Asp gt c Val gt c Val gga Sy acg Thr ggc Sy gat Asp 100 cgg Ar g cac Hi s ggc G y cac Hi s gaa G u 180 ttg Leu ggc Sy ggt Sy gga Sy cac Hi s acg Thr tgg Tr p ggt Sy ttc Phe ctg Leu gag G u ggt G y att SIe att SIe 165 gag G u gtt Val act Thr gaa G u agg Ar g 245 cga gtt agc gtc ggg gcg Arg Val Ser Val Oy Al a ttc ccg atc tcg att Phe Pro lie Ser Ile gat gtt gaa atg ttt Asp Val G u Met Phe Page 23 seql i st cor r ect ed. t xt 250 255 260 ggg gag gcg tcg aaa agc gcc gtt ctt gat ctt tta aaa acg ctc gac 931 Gy G u Ala Ser Lys Ser Ala Val Leu Asp Leu Leu Lys Thr Leu Asp 265 270 275 gag ccg gaa acc gta ttc ctg ggc gtt gac cga ctg gac tac acc aag 979 G u Pro G u Thr Val Phe Leu y Val Asp Arg Leu Asp Tyr Thr Lys 280 285 290 ggc att ttg cag cgc ctg ctt gcg ttt gag gaa ctg ctg gaa tcc ggc 1027 Gy Ile Leu G n Arg Leu Leu Ala Phe G u G u Leu Leu u Ser y 295 300 305 gcg ttg gag gcc gac aaa gct gtg ttg ctg cag gtc gcg acg cct tcg 1075 Ala Leu G u Ala Asp Lys Ala Val Leu Leu G n Val Ala Thr Pro Ser 310 315 320 325 cgt gag cgc att gat cac tat cgt gtg tcg cgt tcg cag gtc gag gaa 1123 Arg G u Arg lie Asp His Tyr Arg Val Ser Arg Ser G n Val G u G u 330 335 340 gcc gtc ggc cgt atc aat ggt cgt ttc ggt cgc atg ggg cgt ccc gtg 1171 Ala Val Gy Arg Ile Asn y Arg Phe y Arg IVet y Arg Pro Val 345 350 355 gtg cat tat cta cac agg tca ttg agc aaa aat gat ctc cag gtg ctg 1219 Val His Tyr Leu His Arg Ser Leu Ser Lys Asn Asp Leu G n Val Leu 360 365 370 tat acc gca gcc gat gtc atg ctg gtt acg cct ttt aaa gac ggt atg 1267 Tyr Thr Ala Ala Asp Val IVet Leu Val Thr Pro Phe Lys Asp Gy IVet 375 380 385 aac ttg gtg gct aaa gaa ttc gtg gcc aac cac cgc gac ggc act ggt 1315 Asn Leu Val Ala Lys G u Phe Val Ala Asn His Arg Asp G y Thr G y 390 395 400 405 gct ttg gtg ctg tcc gaa ttt gcc ggc gcg gcc act gag ctg acc ggt 1363 Ala Leu Val Leu Ser G u Phe Ala Gy Ala Ala Thr G u Leu Thr y 410 415 420 gcg tat tta tgc aac cca ttt gat gtg gaa tcc atc aaa cgg caa atg 1411 Ala Tyr Leu Cys Asn Pro Phe Asp Val u Ser Ile Lys Arg G n IVet 425 430 435 gtg gca gct gtc cat gat ttg aag cac aat ccg gaa tct gcg gca acg 1459 Val Ala Ala Val His Asp Leu Lys His Asn Pro u Ser Ala Ala Thr 440 445 450 cga atg aaa acg aac agc gag cag gtc tat acc cac gac gtc aac gtg 1507 Arg IVet Lys Thr Asn Ser G u G n Val Tyr Thr His Asp Val Asn Val 455 460 465 tgg gct aat agt ttc ctg gat tgt ttg gca cag tcg gga gaa aac tca 1555 Trp Ala Asn Ser Phe Leu Asp Cys Leu Ala G n Ser y G u Asn Ser 470 475 480 485 tgaaccgcgc acgaatcgcg acc 1578 <210> 18 <211> 485 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 18 IVet Asp Asp Ser Asn Ser Phe Val Val Val Ala Asn Arg Leu Pro Val Page 24 seql i st cor r ect ed. t xt Asp Gy Cys Ar g Asp Leu Hi s Val Leu 145 Phe Pr o Gy G n G n 225 Thr Asp Leu Leu Leu 305 Val Ser Thr Leu Val Asp G u Hi s Phe 115 Al a Pr o Leu Ar g Hi s 195 Al a Ser Asp G u Thr 275 Tyr G u Thr Val His Pro Asp y Thr Tr p Gy Phe Leu G u Gy SIle SIle 165 G u Val Thr G u Ar g 245 Phe Asp Lys Gy Ser 325 G u Gy Pr o Val 70 Tyr SIle Val Al a Leu 150 Pr o SIle G n Al a Al a 230 Ar g Gy G u Gy Al a 310 Ar g Al a Leu Gy Leu G u Val Asn Thr 135 Ar g Phe Val Asn Gy 215 Leu Val G u Pr o SIle 295 Leu G u Val Ser 40 Thr Leu Gy Thr Leu 120 Val G n Pr o Ar g Al a 200 Ser Val Ser Al a G u 280 Leu G u Ar g Sy Tyr 360 Ser Pr o Val Hi s Phe Pr o 105 Lys Tr p Met Ser Sy 185 G u Hi s Ar g Val Ser 265 Thr G n Al a SIle Ar g 345 Tyr Ser lie Ser Pro Ser Pro Val Leu G u G n His Arg G y Asp Val Ala Pro u Pro Phe Pro Val Val Leu Thr Ala Ser 75 Ser Asn Ala Thr Leu Trp Pro 90 Val Tyr Asn Thr Asp Trp Trp 110 Phe Ala G u Ala Val Ser G n 125 Val G n Asp Tyr G n Leu Leu 140 Arg Pro Asp Leu Lys lie y 155 160 Pro Asp Leu Phe Arg G n Leu 170 175 IVet Leu G y Al a Asp Leu Val 190 Asn Phe Leu Ala Leu Thr G n 205 Val y G n Pro Asp Thr Leu 220 G u IIe G y Ala His Val G u 235 240 Sy Ala Phe Pro Ile Ser lle 250 255 Lys Ser Ala Val Leu Asp Leu 270 Val Phe Leu y Val Asp Arg 285 Arg Leu Leu Ala Phe G u G u 300 Asp Lys Ala Val Leu Leu G n 315 320 Asp His Tyr Arg Val Ser Arg 330 335 lie Asn Gy Arg Phe y Arg 350 IVet y Arg 355 Pro Val Val His Leu His Arg Ser Leu Ser Lys Asn 365 Page As p Ph e 385 Ar g Thr I Ie G31u Hi s 465 Ser (31n As p G31y Le u Ar g 435 Al a ValI (31u Le u vb t G31y 405 G31y vb t Thr ValI Ser 485 Tyr As n 390 Al a Al a ValI Ar g Tr p 470 Thr 375 Le u Le u Tyr Al a vb t 455 Al a seql i st car r ect ed. t xt Al a Al a Asp Val l'bt Leu 380 Val Al a Lys G31u Phe Val 395 Val Leu Ser G31u Phe Al a 410 Leu Cys As n Pr o Phe As p 425 Al a Val Hi s As p Leu Lys 440 445 Lys Thr As n Ser 03 u 03 n 460 As n Ser Phe Leu As p Cys 475 ValI Al a G31y ValI 430 Hi s ValI Le u Th r As n Al a 415 G31u As n Ty r Al a <210O> 19 <21 1> 1546 <212> DNA <213> Cor ynebact er i urn gI ut anm cumn <220> <221 ODS <222> (1546) <223> FRXA00351 <400> 19 gaaggct gct gct aagaaaa cgaccaagaa gaccact aag cgcaaagaag accacaaaga agtcttaagc cggatcttat aaaactacta aaaagaccac at g gat gat t cc aat 11! l'bt Asp Asp Ser Asn ag c Ser cca Pr o ggg G31y cct Pr o gt t ValI t ac Tyr gt a ValI ggt G31y t cc Ser act Thr ct g Le u ggc (31y gt t ValI t at Tyr gt t ValI g at As p cct Pr o t ca Ser gct Al a ag c Ser ct g Le u gt t ValI gt t ValI 75 aac As n aac As n at c I Ie g aa G31u gca Al a gt c ValI gca Al a ct g Le u ccc Pr o cat Hi s g aa G31u act Thr ct g Le u cca Pr a 15 ag c Ser cgt Ar g cca Pr a gca Al a t gg Tr p 95 gt g ValI ccc Pr a gga G31y Ph e agt Ser cct Pr a at g G31y t gg Tr p ac a Thr t at Tyr tt c Ph e act Thr ct t Le u gt c ValI g at As p g aa G31u c ac Hi s gt c ValI gt c ValI gga G31y ac g Thr ggc G31y g at As p 1 00 att gtt act ccg gtg tac aac acc gat tgg tgg I Ie Val Thr Pro Val Tyr Asn Thr Asp Tr p Tr p cat gcg t tt cgg gag His Ala Phe Arg Gu Page 26 seql i st cor r ect ed. t xt gt a Val gcc Al a ttg Leu 150 ccc Pr o at t SIle caa G n gcc Al a gca Al a 230 cga Ar g ggg Sy gag G u ggc Sy gcg Al a 310 cgt Ar g gcc Al a gt g Val ct c Leu 120 gt g Val cag G n cct Pr o cga Ar g gca Al a 200 tct Ser gt g Val agc Ser gcg Al a gaa G u 280 ttg Leu gag G u cgc Ar g ggc Sy t at Tyr 360 ttc Phe gt g Val cgc Ar g cct Pr o 170 at g let aac Asn gt g Val gag G u ggg Sy 250 aaa Lys gt a Val cgc Ar g gac Asp gat Asp 330 at c SIle cac Hi s gct Al a cag G n cct Pr o 155 gat Asp ct g Leu tt c Phe ggt Gy at t SIle 235 gcg Al a agc Ser tt c Phe ct g Leu aaa Lys 315 cac Hi s aat Asn agg Ar g gaa G u gac Asp 140 gat Asp ct g Leu ggc Gy ctt Leu cag G n 220 ggc G y ttc Phe gcc Al a ct g Leu ctt Leu 300 gct Al a t at Tyr ggt Sy t ca Ser gcc Al a 125 t at Tyr ttg Leu ttc Phe gca Al a gcg Al a 205 ccg Pr o gct Al a ccg Pr o gtt Val ggc G y 285 gcg Al a gt g Val cgt Ar g cgt Ar g ttg Leu 365 110 gt g Val cag G n aag Lys cgt Ar g gat Asp 190 tta Leu gac Asp cat Hi s at c SIle ctt Leu 270 gtt Val ttt Phe ttg Leu gt g Val ttc Phe 350 agc Ser agc Ser ct g Leu at c SIle cag G n 175 ttg Leu acc Thr acc Thr gtt Val tcg Ser 255 gat Asp gac Asp gag G u ct g Leu tcg Ser 335 ggt Sy aaa Lys caa G n ttg Leu ggt Sy 160 ct g Leu gt g Val cag G n ttg Leu gaa G u 240 at t SIle ctt Leu cga Ar g gaa G u cag G n 320 cgt Ar g cgc Ar g aat Asn gcg Al a 130 gtt Val ttc Phe tgg Tr p ttc Phe gtt Val 210 gt c Val gct Al a gtt Val aaa Lys gac Asp 290 ct g Leu gcg Al a cag G n ggg Sy ct c Leu 370 gca Al a cct Pr o ct c Leu cgt Ar g cat Hi s 195 gcc Al a agt Ser gac Asp gaa G u acg Thr 275 t ac Tyr gaa G u acg Thr gt c Val cgt Ar g 355 cag G n cac Hi s ggc Sy cac Hi s gaa G u 180 ttg Leu ggc Sy ggt G y gga G y at g Met 260 ct c Leu acc Thr t cc Ser cct Pr o gag G u 340 ccc Pr o gt g Val 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 tat acc gca gcc gat gtc atg ctg gtt acg Tyr Thr Ala Ala Asp Val IVet Leu Val Thr cct ttt aaa Pro Phe Lys gac ggt at g Asp G y Vet Page 27 375 aac ttg gtg Asn Leu Val 390 gct ttg gtg Ala Leu Val gcg tat tta Al a Tyr Leu gt g gca gct Val Al a Al a 440 cga atg aaa Arg IVet Lys 455 tgg gct aat Trp Al a Asn 470 <210> <211> 482 <212> PRT aaa Lys t cc Ser 410 aac Asn cat Hi s aac Asn ttc Phe gaa Gu 395 gaa G u cca Pr o gat Asp agc Ser ct g Leu 475 380 ttc Phe ttt Phe ttt Phe ttg Leu gag G u 460 gat Asp seql i st cor r ect ed. t xt 385 gtg gcc aac cac cgc gac Val Ala Asn His Arg Asp 400 gcc ggc gcg gcc act gag Al a y Al a Al a Thr G u 415 gat gtg gaa tcc atc aaa Asp Val u Ser Ile Lys 430 aag cac aat ccg gaa tct Lys His Asn Pro u Ser 445 450 cag gtc tat acc cac gac G n Val Tyr Thr His Asp 465 tgt ttg gca cag tcg gga Cys Leu Al a G n Ser G y 480 ggc G y ct g Leu cgg Ar g 435 gcg Al a gt c Val act Thr acc Thr 420 caa G n gca Al a aac Asn 1315 1363 1411 1459 1507 1546 <213> Corynebacter i um gl ut ai cum <400> IVet Asp Asp Ser Asn Ser 1 Asp Sy Cys Ar g Asp Leu Hi s Val Leu 145 Phe Thr Leu Val Asp G u Hi s Phe 115 Al a Pr o Leu 5 Hi s Thr Tr p Sy Phe Leu G u Sy SIle SIle 165 Pr o Sy Pr o Val 70 Tyr SIle Val Al a Leu 150 Pr o Phe Asp Leu Sy Leu G u Val Asn Thr 135 Ar g Phe Val Val 10 Ser Tyr Pro Val Val Asp His Pr o Phe Ser 90 Pro Val 105 Lys Phe Trp Val Ivet Arg Ser Pro 170 G y Met 185 Al a Ser Leu Val Val 75 Asn Tyr Al a G n Pr o 155 Asp Asn SIle G u Al a Val Al a Asn G u Asp 140 Asp Leu Ar g Ser G n Pr o Leu Thr Thr Al a 125 Tyr Leu Phe Leu Pro Val Pro Ser Pro His Arg G y G u Pro Phe Thr Al a Ser Leu Trp Pro Asp Trp Trp 110 Val Ser G n G n Leu Leu Lys I e G y 160 Arg G n Leu 175 Asp Leu Val 190 Pro Trp Arg G u I e Val Arg Leu G y Al a Page 28 seqi i st car r ect ed. t xt G31y Phe Hi s Leu Val O31n Asn Ala O31u Asn Phe Leu Ala Leu Thr O31n 195 200 205 O31n O31n 225 Thr As p Le u Le u Le u 305 ValI Ser vb t As p Ph e 385 Ar g Thr I Ie O31u Hi s 465 Ser A a Ser As p O31u Thr 275 Tyr O31u Thr ValI Ar g 355 O31n As p O31y Le u Ar g 435 A a ValI Thr O31u Ar g 245 Ph e As p Lys5 O31y Ser 325 O31u ValI Le u vb t O31y 405 O31y vb t Thr ValI A a A a 230 Ar g O31y O31u O31y A a 310 Ar g A a ValI Tyr As n 390 A a A a ValI Ar g Tr p 470 O31y 215 Le u ValI O31u Pr o I Ie 295 Le u (31u ValI Hi s Thr 375 Le u Le u Tyr A a vb t 455 A a Ser ValI Ser A a O31u 280 Le u O31u Ar g O31y Tyr 360 A a ValI ValI Le u A a 440 Lys5 As n Hi s Ar g ValI Ser 265 Thr O31n A a I Ie Ar g 345 Le u A a A a Le u cys 425 ValI Thr Ser Oy le 235 A a Ser Ph e Le u Lys5 315 Hi s As n Ar g ValI O31u 395 O31u Pr o As p Ser Le u 475 O31n 220 O31y Ph e A a Le u Le u 300 A a Tyr O31y Ser vb t 380 Ph e Ph e Ph e Le u O31u 460 As p Pr o A a Pr o ValI O31y 285 A a ValI Ar g Ar g Le u 365 Le u ValI A a As p Lys5 445 O31n Cys Thr ValI Ser 255 As p As p O31u Le u Ser 335 O31y Thr As n A a 415 O31u As n Ty r A a <210O> 21 <21 1> 779 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (756) <223> RXA00873 Page 29 seqi i st car r ect ed. t xt <400> 21 act Thr g aa O31u t cc Ser cca Pr o tt g Le u ggc O31y ccc Pr o cct Pr o gcc A a cgc Ar g 145 ggt O31y ac a Thr at g vb t gga O31y gct A a 225 tt t Ph e c aa O31n gt c ValI cct Pr o ct t Le u t ct Ser ggc O31y c ag O31n cgc Ar g 115 gt a ValI ct c Le u gga O31y tt a Le u aaa Ly s 1 95 ac c Thr cgg Ar g ct c Le u ggt O31y g at As p at g vb t ggc O31y cct Pr a aat As n t cc Ser t ac Tyr gt g ValI aac As n t ac Tyr 1 65 g aa O31u cct Pr a cga Ar g t gg Tr p gcc A a 245 at t I Ie gag O31u cgc Ar g gac As p ggc O31y 70 at c I Ie aac As n ct t Le u gaa O31u caa O31n 150 cgt Ar g cac Hi s cag O31n gag O31u at t I Ie 230 g at As p tt c Ph e g aa O31u gcc A a cgc Ar g t ca Ser t gg Tr p g ac As p cca Pr a ag c Ser 135 at c I Ie g aa O31u aag Ly s gca A a at g vb t 215 gt c ValI g aa O31u ct g Le u cgc Ar g aac As n 40 aac As n ccc Pr a ct c Le u cgc Ar g gcg A a 120 c aa O31n ct t Le u gt g ValI O31y gt c ValI 200 t cg Ser act Thr aag Ly s cgt Ar g ag c Ser 25 gt a ValI c ag O31n gt g ValI c ac Hi s aac As n 1 05 at c I Ie ct c Le u at c I Ie t cc Ser c aa O31n 1 85 t cg Ser ggc O31y tt a Le u g ac As p aat As n 10 t ac Tyr gga O31y ct g Le u tt g Le u g ac As p 90 ggt O31y c aa O31n aac As n cgc Ar g t cc Ser 1 70 ac c Thr ct t Le u ggg O31y gcc A a g at As p 250 cat Hi s at g vb t at c I Ie g aa O31u t at Tyr cgc Ar g ggt O31y aat As n cgc Ar g aag Ly s 1 ac c Thr at t I Ie g at As p c ag O31n cct Pr a 235 at g vb t gag O31u t cc Ser ag g Ar g ct t Le u ggt O31y gga O31y t cc Ser c aa O31n 125 aac As n t ac Tyr gag O31u t gt cys cgt Ar g 205 tt c Ph e gga O31y ct c Le u c aa O31n cgc Ar g c ac Hi s g at As p gt g ValI aaa Ly s 110 t ac Tyr t cc Ser cgc Ar g t ca Ser gt c ValI 1 g aa O31u cct Pr a tt c Ph e ac c Thr tt c Ph e ct t Le u ggt O31y g aa O31u cgc Ar g gct A a ggc O31y ct g Le u gca A a gt g ValI 1 aac As n tt t Ph e ac c Th r tt c Ph e t gagcat t gg ccaacacat c at c Page seqI i st car r ect ed. t xt <210O> 22 <21 1> 252 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 22 Th r Ala O31n Tr p O31u Ser Pr o Le u G31y Pr o Pr o A a Ar g 145 G31y Th r vb t G31y A a 225 Ph e ValI Pr o Le u Ser G31y G n Ar g 115 ValI Le u G31y Le u Ly s 1 95 Th r Ar g Le u G31y As p vb t G31y Pr a As n Ser Ty r ValI As n Ty r 1 65 O31u Pr a Ar g Tr p A a 245 O31u A a Ar g Ser Tr p As p Pr a Ser 135 li1e O31u Ly s A a vb t 215 ValI O31u Ilie Phe Leu Arg Ser ValI O31n ValI Hi s As n 1 li1e Le u I Ie Ser O31n 1 Ser G31y Le u As p As n 10 Ty r G31y Le u Le u As p 90 G y O31n As n Ar g Ser 1 70 Th r Le u G31y A a As p 250 Hi s vb t I Ie O31u Ty r Ar g G y As n Ar g Ly s 1 Th r I Ie As p O31n Pr a 235 vb t O31u Ser Ar g Le u G31y G31y Ser O31n 125 As n Ty r O31u cys Ar g 205 Ph e O31y Th r Ph e Le u O31y O31u Ar g A a O31y Le u A a ValI 1 As n Ph e Th r Ph e <210O> 23 <21 1> 1102 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (1102) <223> RXA00891 <400> 23 t caat at t cc gaagaaaacc gcgcagct ct ct cact agt c t caggt gagg cgaaagt ggt Page 31 seqi i st car r ect ed. t xt gaaagacccg ctacgcatgg tgcgcctggc tttttagaat t gg Tr p t ct Ser ct t Leu gt c Val gag (31u t ac Tyr aaa Lys ccg Pr o ttc Phe ct C Leu 150 gt c Val cgc Ar g ccc Pr o t cc Ser ttc Phe 230 gag (31u ttc Phe ccg Pr o gcc Al a gca Al a t ac Tyr cca Pr o gat Asp t at Tyr 120 gaa O31u gac Asp aat Asn cca Pr a ct g Leu 200 t gg Tr p t cc Ser at g IN/bt at c I e gca Al a ggc G31y gct Al a gac Asp ggc G31y at c l Ie t cc Ser ct g Leu gcc Al a acc Thr 170 ggc G31y aac Asn t at Tyr caa O31n gat Asp 250 gca Al a cac Hi s at c l Ie ggt G31y gt t Val gga G31y t gg Tr p ct g Leu gaa (31u 140 cgc Ar g gac Asp t ac Tyr gcc Al a ccg Pr a 220 gac Asp ttg Leu at g lvb t cct Pr a gag O31u cag O31n t ac Tyr gga G31y ggc G31y 110 gac Asp ggc G31y ggc G31y cac Hi s gat Asp 190 at c l Ie cgt Ar g aac Asn ttc Phe gat Asp cac Hi s g aa O31u t gg Tr p gt g Val 80 ttg Leu gat Asp ggt G31y gt c Val cgt Ar g 160 t gg Tr p t at Tyr ttt Phe cag O31n gac Asp 240 ct g Leu gt g Val 1 acc Thr gcg Al a t cg Ser gag O31u ct g Leu aaa Lys t gc Cys t ac Tyr gat Asp 145 gt t Val ttc Phe gt g Val gt a Val t ac Tyr 225 aac Asn gac Asp Leu 3 n tct ccg Ser Pro gct cgc Ala Ar g gaa gag aag aaa Lys Lys gtt cgt Val Arg ggc ctg O y Leu att tgg I Ie Trp 115 gat atc Asp II e 130 gac ttc Asp Phe atc acc II e Thr caa gaa tgg agc Trp Ser 195 gat aca Asp Thr 210 ttc tgg Phe Trp ccc gca Pr o Al a ctg gga Leu 03 y Thr ttg Leu cca Pr a ttt Phe aac Asn gcc Al a acc Thr 100 at c l Ie cgc Ar g gt g Val gac Asp t cc Ser 180 gat Asp g aa O31u cac Hi s gt c Val ct t Leu 260 ctg caa acc tcc Ser aat Asn gt t Val cca Pr a cgc Ar g ttc Phe gaa O31u cca Pr a aac Asn gaa O31u ttg Leu 165 cgg Ar g gat Asp g aa O31u cgc Ar g caa O31n 245 gat Asp Page 32 ggt G31y aac As n t ct Ser aac As n 310 ggC G31y cga Ar g g aa O31u 280 at t I Ie t gg Tr p g aa a U g at As p ct c Le u aag Ly s c aa O31n C ac Hi s 330 gcc A a aaa Ly s gaa O31u g at As p 315 at g b t gt t ValI g aa O31u t ac Tyr 300 gt g ValI gcc A a seql i st car r ect ed. t xt cct t at ct t t tt gaa cgc gaa ggc Pr o Ty r Leu Phe O31u Ar g (31u (31y 270 275 acc cac gat t tc ct c aaa ct g t gt Thr Hi s As p Phe Leu Lys Leu Cys 285 290 ccc ggc cga atc c tg ct C gca gaa Pro G31y Ar g Ilie Leu Leu Ala O31u 305 gt c gaa tac t tc ggt gaa aaa gac Val O31u Tyr Phe G31y O31u Lys Asp 320 ttc cac Phe Hi s ac c Thr cgc Ar g gcc A a aaa Ly s 325 931 979 1 027 1 075 1102 <210O> 24 <21 1> 334 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ari cumn <400> 24 Val Leu O31n Thr Ser Tr p Hi s Phe Ser I Ie Leu Ala G31y I'bt Thr Asp Thr A a Ser O31u Le u Ly s Cys Tyr As p 145 ValI Ph e ValI Pr o Ar g O31u Ly s Ar g Le u Tr p 115 li1e Ph e Thr O31u Ser 1 95 As n ValI Pr o Ar g Ph e O31u Pr o As n O31u Le u 1 65 Ar g As p O31n As p Pr o Tr p As p Le u Ph e Ar g 135 ValI vb t As p Thr Pr a A a 40 A a Tyr Pr a As p Tyr 120 (31u As p As n Pr a Le u 200 10 Se r A a Hi s 03 y Pr o Ala Lys As p O31u (1y 90 Ty r I Ie 1 Asp Ser I I e Leu Hi s A a Hi s Thr 1 70 Thr 03 y 1 Ty r As n As p Le u Pr a A a As n O31n Pr a Pr a Hi s 1 Ser Pr a (31u Hi s I Ie O31y ValI O31y Tr p Le u O31u 140 Ar g As p Tyr A a Hi s ValI O31u Ph e ValI Le u Ar g 125 Ph e Ar g O31n O31y Ar g 205 As p Hi s Pr a O31u Ser ValI O31y Thr Le u A a 1 Ph e I Ie Val Asp Thr Ou Ou Ser Asn Trp Thr Tyr Asp Pro Val Arg Oy On Page 33 Tyr 225 As n As p (31u Le u Le u 305 G31y Tr p A a G31y O31u 275 Le u A a Ly s Ar g (31n 245 As p Thr Ar g A a Ly s 325 215 Ph e A a Ph e G31y ValI 295 O31n As p seql i st car r ect ed. t xt 220 Ser Hi s O31n Pro Asp Leu 235 N/t Leu As p Val Leu Arg 250 Ar g Leu As p Ala Val Pr o 265 O3 u As n Leu Lys 03 u Thr 280 285 li1e O31u Lys O31u Ty r Pr o 300 Tr p Pr o O31n As p Val Val 315 O3 u Cys Hi s l'b t A a Phe 330 Ty r Tr p 255 Le u As p Ar g Ty r <210O> <21 1> 1386 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (1363) <223> RXA00534 <400> ct gt gcagaa agaaaacact cctct ggct a ggt agacaca gcgggt aact gt cagcacgt agat cgaaag gt gcacaaag gt t tataaag gt agagt tga c ag O31n gt c ValI gt t ValI gca A a ct g Le u gag O31u gt g ValI t at Ty r g aa O31u t gc Cys gca A a gct A a ct t Le u ac c Th r ggt G31y at c I Ie gca A a aat As n gag O31u gca A a gag (31u t cc Ser gt t ValI at g vb t CCC Pr o cgt Ar g 75 gaa O31u cgc Ar g t cg Ser gcc A a gga G31y gt t ValI at t I Ie gcc A a c ac Hi s ct t Le u ac c Th r g ac As p 45 ccg Pr o t ct Ser c aa O31n gga (31y gag O31u aag Lys5 ac c Th r cca Pr o aac As n t ct Ser aac As n 110 agt Ser 15 aag Lys5 ac g Th r gct A a gct A a tt c Ph e 95 gca A a gcg A a gct A a g at As p cgt Ar g ct c Le u 80 ac g Th r cgc Ar g gt g ValI 1 g aa O31u gga O31y g aa O31u g aa O31u gt c ValI ggc O31y at t I Ie gcc A a cgc Ar g aat As n ct t Le u at g vb t gcc A a t ct Ser gt t ValI gt c ValI ag a Ar g gt c ValI g aa O31u at g vb t gct A a gct A a 1 00 gt c ValI gt a ValI aac As n gt g ValI ct t Le u ct c Le u at t I Ie ggt O31y act Th r Page 34 seql i st cor r ect ed. t xt cca ggt cgt Pro G y Ar g 120 gtg cgt gaa gca Val Arg u Ala gct Al a ggt Sy 150 aac Asn gct Al a ttc Phe ct g Leu cgc Ar g 230 gag G u aag Lys gag G u gac Asp at c SIle 310 ttg Leu gac Asp cca Pr o aac Asn ttc Phe ggt Sy gat Asp ccg Pr o gaa G u 200 agt Ser tct Ser at t SIle gaa G u gcg Al a 280 gtt Val ttc Phe aag Lys gt c Val gtt Val 360 gaa Gu ggt Sy tct Ser tgt Cys 170 at c SIle ct g Leu gaa G u agt Ser gt g Val 250 aaa Lys gtt Val cag G n tgc Cys cag G n 330 aaa Lys gca Al a at t SIle gtt Val gac Asp 155 gag G u gtt Val gaa G u t ac Tyr aat Asn 235 gaa G u gt a Val tt c Phe aac Asn cct Pr o 315 gtt Val gt c Val gag G u t cc Ser aat Asn 140 acc Thr at t SIle cct Pr o ctt Leu gct Al a 220 gat Asp gaa G u acc Thr cgt Ar g gt c Val 300 cgt Ar g cag G n t cc Ser ttc Phe acc Thr 380 ct c Leu 125 aaa Lys act Thr t ac Tyr aat Asn gct Al a 205 cgt Ar g ccc Pr o gca Al a gtt Val gcg Al a 285 tct Ser t cc Ser ggc G y ct c Leu at g Met 365 tct Ser gaa G u gca Al a tcg Ser gca Al a 190 gct Al a gca Al a ggc Gy gt c Val ct g Leu 270 ttg Leu tct Ser gac Asp aac Asn gt g Val 350 gaa G u gag G u acc Thr gtt Val gac Asp 175 cag G n gtt Val ttc Phe act Thr ctt Leu 255 ggt G y gct Al a gt a Val ggc G y tgg Tr p 335 ggt G y gct Al a cgc Ar g gcg Al a 160 gtt Val aag Lys ggc G y aat Asn ttg Leu 240 acc Thr at t SIle gat Asp gaa G u cgc Ar g 320 acc Thr gct Al a ct g Leu gat gag ggc Asp G u y aag at c t gc Lys II e Cys 130 gat gt c acc Asp Val Thr 145 ttg gca gct Leu Al a Al a gac ggt gt g Asp G y Val ct g aa aag Leu G u Lys 195 tcc aag att Ser Lys Ile 210 gt g cca ct t Val Pro Leu 225 att gcc ggc i e Al a G y ggt gtc gca Sy Val Ala t cc gat aag Ser Asp Lys 275 gca gaa at c Ala G u Ile 290 gac ggc acc Asp G y Thr 305 cgc gcg atg Arg Ala IVet aat gtg ct t Asn Val Leu ggc atg aag G y IVet Lys 355 cgc gat gtc Arg Asp Val 370 at t SIle acg Thr gct Al a t at Tyr 180 ct c Leu ttg Leu cgc Ar g tct Ser acc Thr 260 cca Pr o aac Asn acc Thr gag G u t ac Tyr 340 tct Ser aac Asn gtt Val ttg Leu ttg Leu 165 acc Thr agc Ser gt g Val gt a Val at g Met 245 gac Asp ggc G y at t SIe gac Asp at c SIe 325 gac Asp cac Hi s gt g Val 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 att cgt att tcc gtg ctg atc Ile Arg Ile Ser Val Leu Ile 385 Page seqI i st car r ect ed. t xt cgt gaa gat gat ct g gat gct gct gca cgt gca t tg cat gag cag t tc Ar g O31u As p As p Leu As p Ala Ala Ala Ar g Ala Leu His Ou O31n Phe 390 395 400 405 cag ctg ggc ggc gaa gac gaa gcc gtc gtt tat gca ggc acc gga cgc O31n Leu G31y 31y O31u Asp Ou Ala Val Val Tyr Ala G31y Th r G31y Ar g 410 415 420 t aaagtt t ta aaggagt agt tt t <210> 26 <211> 421 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 1315 1363 1386 <400> 26 Val A a Leu Val Val O31n Lys Tyr G31y O31u G31y O31u O31u ValI G31y I Ie As p 145 Le u As p Le u Ser ValI 225 li1e I Ie As p Le u As p vb t O31n As p 115 cys Thr A a ValI Lys5 1 95 li1e Le u G31y As n ValI Le u Le u I Ie G31y Thr ValI Le u Le u 1 65 Thr Ser ValI ValI vb t 245 A a ValI A a Thr Ser Le u G31y G31y 135 Ar g A a As p O31u Ar g 215 Ser As p O31u cys 40 A a A a Le u Thr Ar g 120 Ph e G31y As p Pr a O31u 200 Ser Ser I Ie Ar g Ser ValI G31y G31y Thr 1 05 ValI O31n G31y ValI Ar g 1 85 vb t ValI Tyr Pr a G31y 10 I Ie A a As n O31u A a 90 O31u Ar g G31y Ser cys 1 70 li1e Le u O31u Ser ValI 250 Ser ValI vb t Pr a Ar g O31u Ar g O31u ValI As p 1 O31u ValI O31u Tyr As n 235 (31u Ser A a G31y ValI li1e A a Hi s A a As n 140 Thr I Ie Pr a Le u A a 220 As p (31u Le u Thr As p Pr a Ser O31n O31y Le u 125 Lys5 Thr Tyr As n A a 205 Ar g Pr a A a O31u Lys5 Thr Pr a As n Ser As n 110 As p O31u A a Ser A a 1 A a A a O31y ValI Ser Thr A a A a Ph e A a O31u Thr ValI As p 1 O31n ValI Ph e Thr Le u 255 (3y Val Ala Thr Asp Lys Ser (3u Ala Lys Val 260 265 Thr Val Leuc3y Ilie 270 Page 36 Ser A a As p 305 Ar g As n G31y Ar g I Ie 385 Le u A a Ly s 275 li1e Thr vb t Le u Ly s 355 ValI ValI G u Thr Gy le As p I Ie 325 As p Hi s ValI I Ie Ph e 405 Ar g A a vb t 295 Thr Ly s O31n G31y I Ie 375 O31u Le u seqI i st car r ect ed. t xt Ala Lys Val Phe Ar g Ala 280 285 Val Leu O31n As n Val Ser 300 Phe Thr Cys Pr o Ar g Ser 315 Lys Leu O31n Val O31n G31y 330 Val 03 y Lys Val Ser Leu 345 Val Thr Ala O31u Phe I'b t 360 365 03 u Leu I I e Ser Thr Ser 380 As p As p Leu As p A a A a 395 G y G y G u As p G u A a 410 Le u Ser As p As n ValI 350 O31u O31u A a ValI A a ValI O31y Tr p 335 O31y A a I Ie Ar g ValI 415 As p O31u Ar g 320 Thr A a Le u Ar g A a 400 Tyr <210O> 27 <21 1> 1155 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (1132) <223> RXA00533 <400> 27 ct gcacgt gc at tgcat gag cagt tccagc t gggcggcga caggcaccgg acgct aaagt t t taaaggag t agtt t taca gt t ValI g aa O31u cgt Ar g g aa O31u gcg A a ggt O31y cgc Ar g gca A a at t I Ie tt c Ph e ac c Thr tt c Ph e cgt Ar g c ag O31n gct A a ggc O31y cca Pr o aag Ly s gca A a gga G31y 75 c ag O31n gct A a at t I Ie ac c Thr ggc (31y gt c ValI g ac As p g aa O31u 45 gag O31u ac c Thr ggc O31y act Thr tt c Ph e gag O31u gct A a cag gtt 03 n Val 15 gt t cgt Val Ar g cgt ggc Ar g O31y t cc ct c Ser Leu t cc aag Ser Lys 80 Page 37 agacgaagcc gt cgt t t at g at g acc acc at c gca I'b t Thr Thr Ilie Ala 1 at g cgc acc ct t tt g I'b t Ar g Thr Leu Leu ttc ttt gct tcc cca Phe Phe A a Ser Pr o acg gaa atc gag gta Thr 03 u Il e 03 u Val aag gac atc gac gtt Lys Asp Il e Asp Val cag t ac gct cca ct g (31n Tyr Ala Pr o Leu seql i st cor r ect ed. t xt ttc gct gct gca Phe Al a Al a Al a aag Lys aag Lys at g Met gt a Val 150 gca Al a aac Asn gga Gy aac Asn cgc Ar g 230 ggc Gy cac Hi s ttg Leu gca Al a t cc Ser 310 aac Asn gac Asp t cc Ser 120 gcg Al a ctt Leu gt g Val gag G u t at Tyr 200 gt c Val gaa G u tgc Cys gaa gcc Al a 280 gcc Al a gt c Val cgc Ar g ggc Gy gtt Val gt c Val cca Pr o gtt Val acc Thr 170 gt c Val t ca Ser gac Asp cgc Ar g cgc Ar g 250 gac Asp t ca Ser at t l Ie gat Asp ggt Gv gcg act gtt gtg Al a Thr Val Val cca Pr o aag Lys gt g Val t cc Ser 155 tt g Leu cat Hi s cca Pr o ggc Gy aag Lys 235 gt g Val aag Lys ggc G y gac Asp aac Asn 315 gct Al a ct a Leu ggc G y ct g Leu 140 tct Ser gca Al a gat Asp at c SIle acc Thr 220 at t SIle ccg Pr o gca Al a gt c Val gaa G u 300 cgc Ar g gcg Al a at c SIle at t SIle 125 aag Lys t ac Tyr aag Lys gga Gy gct Al a 205 ttc Phe ct c Leu gtt Val at c l Ie aag Lys 285 t cc Ser ggt G y ct a gt c Val 110 at t SIle cca Pr o cag G n cag G n cag G n 190 t ac Tyr gaa G u ggt Gy ttc Phe acc Thr 270 ctt Leu ct c Leu ct g Leu aac aac tct tct gct Asn Ser Ser Ala gag gtg aac cct Su Val Asn Pro 115 aac cct aac tgc Asn Pro Asn Cys 130 cac gat gcc gct His Asp Ala Ala 145 gtt tcc ggt tct Val Ser G y Ser 160 gct gca gtt gga Ala Ala Val y gct gac gca ggc Al a Asp Al a G y 195 gtg ctg cca ttc Val Leu Pro Phe 210 gat gaa gag cag Asp G u u n 225 cca gac ctc aag Pro Asp Leu Lys 240 ggc cac acg ctg G y His Thr Leu gac cag gcg cag Asp G n Al a n 275 gac gtc cca acc Asp Val Pro Thr 290 gga cgc atc cgt Gy Arg lie Arg 305 ct c gt c gt a t ct Leu Val Val Ser 320 tgg Tr p 100 t cc Ser acc Thr ggt G y ggt G y gac Asp 180 gat Asp gcc Al a aag Lys gt c Val acc Thr 260 gag G u cca Pr o cag Sn ggc G y 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1155 LVS 330 Leu Asn Thr 335 atc cag atc gct gag ct g Ile n Ile Ala u Leu 340 ctg gtt aag Leu Val Lys taaaaacccg ccattaaaaa ctc Page 38 seql i st cor r ect ed. t xt <210> 28 <211> 344 <212> PRT <213> Corynebact eri um gl ut arri cum <400> 28 Met Thr Thr 1 Vet Arg Thr Phe Phe Al a Thr G u li e Lys Asp II e G n Tyr Al a Ser Ser Al a Val Asn Pro 115 Pro Asn Cys 130 Asp Al a Al a 145 Ser G y Ser Ala Val y Asp Al a G y 195 Leu Pro Phe 210 Su u n 225 Asp Leu Lys Hi s Thr Leu G n Al a G n 275 Val Pro Thr 290 I le Ala Val 5 Leu Leu G u Ser Pro Ar g Su Val u Asp Val Al a 70 Pro Leu Phe Trp Arg Lys 100 Ser Asp Lys Thr Thr bet G y Leu Val 150 G y Leu Al a 165 Asp His Asn 180 Asp Val G y Al a y Asn Lys Leu Arg 230 Val Ser G y 245 Thr Ile His 260 G u Ile Leu Pro Leu Ala Val y Ala Thr 10 G u Arg Asn Phe Ser Ala y Arg 40 Asp Ile Thr G n 55 Leu Phe Ser Al a Al a Al a Al a G y 90 Asp Asp G u Val 105 Asp Ser Leu Val 120 Al a Al a Vet Pr o 135 Lys Leu His Val Sy Val G u Thr 170 Val G u Phe Val 185 Pro Tyr Val Ser 200 Leu Val Asp Asp 215 Asn u Ser Arg Thr Cys Val Arg 250 Ala G u Phe Asp 265 G y Al a Al a Ser 280 Al a Al a G y lile 295 Sy Pr o Lys Al a Sy 75 Al a Pr o Lys Val Ser 155 Leu Hi s Pr o Sy Lys 235 Val Lys Sy Asp G n Al a SIle Thr Sy Thr Leu Sy Leu 140 Ser Al a Asp SIle Thr 220 I le Pr o Al a Val G u 300 Val Asp G u G u Thr Val SIle SIle 125 Lys Tyr Lys Sy Al a 205 Phe Leu Val l Ie Lys 285 Ser G n Val Ar g Ser Ser Asp Ser Al a Leu Al a Val 175 Al a Asn Thr Leu Thr 255 Val Val Val Val Ar g Sy Leu Lys Asn G u Asn Hi s Val 160 Al a Al a Val Asp Pr o 240 Sy Asp Asp Sy Leu 320 Ar g 305 lie Arg G n Asp Ser Thr Val Asp Asp Asn Arg y Leu Val 310 315 Page 39 seql i st cor r ect ed. t xt Val Val Ser y Asp Asn Leu Arg Lys y Ala Ala Leu Asn Thr Ie 325 330 335 Sn Ile Ala G u Leu Leu Val Lys 340 <210> <211> <212> <213> <220> <221> <222> <223> 29 608 DNA Cor ynebact eri um gl ut am cum CDS (608) RXA02843 <400> 29 cccattgcgc ggaggtcgca ccccttccga cttgaact ga taggccgata gaaat t at t c tggacgtc at g Met 1 act act gct tcc gca acc gga att gca aca ctg acc tcc Thr Thr Ala Ser Ala Thr Gy I e Ala Thr Leu Thr Ser acc Thr ggc gac gtc ctg G y Asp Val Leu gtg tgg tat cca Val Trp Tyr Pro gaa Gu atc ggg tcc acc I e G y Ser Thr cag tcc gcg ctc G n Ser Ala Leu aca Thr cct cta gaa ggc Pro Leu G u G y gat gaa gat cga Asp u Asp Arg aac gtc Asn Val acc cgc aaa Thr Arg Lys gac acc tac Asp Thr Tyr gtg acg aca act Val Thr Thr Thr at c I e gac acc gac gca Asp Thr Asp Ala gcc ccc acc Ala Pro Thr cac cgc gtt His Arg Val gat gca tgg ct g Asp Ala Trp Leu ctt cac ctc ctc Leu His Leu Leu t cc Ser ttc cgc Phe Arg cct cac acc atc Pro His Thr Ile aac Asn cta gac ggc att Leu Asp G y II e ggc ctc ctc aac G y Leu Leu Asn aat Asn gtc gtg tgg acc Val Val Trp Thr ttc gga ccg tgc Phe y Pro Cys gca Al a 105 gtt gac ggt ttc Val Asp Gy Phe gca Al a 110 ctc acc cgc gcg Leu Thr Arg Ala cgc Ar g 115 ctg tca cgc cga Leu Ser Arg Arg caa gtt acg gtt G n Val Thr Val tat agc Tyr Ser 125 gtc gac aag Val Asp Lys cgc atc ggt Arg lie G y 145 cca cgc atg gtc Pro Arg IVet Val gac tat gtg gtt ccc tcg ggc gtg Asp Tyr Val Val Pro Ser Gy Val 135 140 gac gcc gac cgc Asp Ala Asp Arg cga ctt ggc gcg Arg Leu y Al a t ac Tyr 155 ct g gca gat Leu Al a Asp ggc acc G y Thr 160 acc gtg atg cat Thr Val Met Hi s gag 15u 165 ggc ttc gtg aac Sy Phe Val Asn ttc Phe 170 aac gct ggc acg Asn Al a y Thr ct c Leu 175 ggc gct tcc atg G y Al a Ser bet Page seql i st cor r ect ed. t xt <210> <211> 180 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> Mat Thr Thr 1 Asp Al a Lys Tyr Pr o Val Ar g Lys Gy 145 Thr Al a Leu Thr Val Al a Thr Thr Ar g 115 Pr o Al a bet Vet Ala Ser Ala Thr Gy I e Al a 5 10 Asp Val Trp Tyr Pro G u lie 25 Pro Leu G u y Val Asp G u 40 Thr Thr Thr Ile Asp Thr Asp Trp Leu Arg Leu His Leu Leu 70 I le Asn Leu Asp G y I e Phe 90 Asn Phe G y Pro Cys Al a Val 100 105 Leu Ser Arg Arg G y G n Val 120 Arg bet Val Asp Tyr Val Val 135 Asp Arg Val Arg Leu y Al a 150 Thr Gy Asp Al a Ser Gy Asp Thr Pr o Tyr 155 Thr Thr Asn Pr o Ar g Leu Phe Tyr 125 Gy Al a Ser Asp Val Thr Val Asn Al a 110 Ser Val Asp Thr G n Thr Asp Phe Asn Leu Val Ar g Gy Hi s G u 165 Val 180 G y Phe Val Asn Phe 170 Asn Al a y Thr Leu G y 175 <210> 31 <211> 1230 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1207) <223> RXA02022 <400> 31 tatttgcgat tccaactgct tgggctccgc gaatgttttc actcattttt taatcgaccg cttccatcat gttttaacta aggtttgtag gcttaaacct gtg aac tct gaa ctc 115 Val Asn Ser G u Leu 1 aaa cca gga tta gat ctc ctc ggc gac cca att gtc ctt act caa cgt 163 Lys Pro G y Leu Asp Leu Leu Gy Asp Pro lie Val Leu Thr G n Arg 15 ttg gta gat ata ccg agt ccg tcg ggt cag gaa aag cag att gct gat 211 Leu Val Asp lie Pro Ser Pro Ser G y n G u Lys G n le Ala Asp 30 Page 41 seql i st cor r ect ed. t xt gaa Gu ttc Phe tcg Ser aat Asn gt c Val ttg Leu gag Gu gat Asp 150 cct Pr o aag Lys ggt Gy gcg Al a ggc G y 230 cca Pr o gat Asp ggt Sy ctt Leu gaa Gu ttc Phe gt c Val cca Pr o at g Met acg Thr 120 gag Gu cat Hi s ggc Gy acg Thr aat Asn 200 aag Lys aac Asn ct c Leu aac Asn gac Asp 280 ggc G y gcc Al a aac Asn ctt Leu cgt Ar g tct Ser act Thr gtt Val gag Gu tgg Tr p 170 cat Hi s at g Met gca Al a gtt Val tgg Tr p 250 gcg Al a at c SIle ggg G y ctt cgg aac ctt aat ct a cct J Asn Leu Pro Leu aac Asn gct Al a 75 gt g Val ggg G y gag G u gct Al a tgg Tr p 155 at t SIle ggt Gy cat Hi s gaa Gu tt c Phe 235 at g Met at c l Ie gaa Gu cag G n Ar g gtt Val ggt G y gaa Gu ttg Leu ctt Leu gat Asp 140 ttg Leu gag Gu gt g Val aag Lys gt c Val 220 tgc Cys aac Asn gag G u tgg Tr p cag G n 300 Asn 45 ctt Leu cat Hi s gac Asp gcg Al a aaa Lys 125 cac Hi s gcg Al a gcg Al a cgt Ar g ttg Leu 205 aac Asn gaa Gu ct c Leu cat Hi s gcc Al a 285 gt g Val gct Al a at c SIle ggc G y gt g Val 110 cat Hi s ct c Leu gct Al a ggc Gy gcc Al a 190 tcg Ser at t SIle tcg Ser aac Asn gt c Val 270 gt a Val aca Thr cgc Ar g gat Asp at c SIle 95 t at Tyr gat Asp aat Asn gat Asp tgc Cys 175 cat Hi s ccg Pr o gat Asp G y ttc Phe 255 gt c Val gaa Gu acg Thr aca Thr at g bet ttg Leu ct g Leu ggt Gy ttg Leu 160 cag G n tcg Ser at c SIle ggc G y gt g Val 240 cgt Ar g gaa Gu gac Asp aac Asn gt g Val t at Tyr cat Hi s acg Thr ttg Leu 145 gcg Al a ggc Gy gcg Al a at t lile ttg Leu 225 gca Al a ttc Phe acg Thr ggg G y ggt Gy agg Ar g ccg Pr o ggc G y act Thr ct g Leu 130 ggc Gy ttg Leu aat Asn aga Ar g tcg Ser 210 acc Thr aac Asn gcg Al a ctt Leu gca Al a 290 gt a Val gga G y at c SIle tgt Cys ttt Phe 115 at t SIle cac Hi s ttg Leu ct g Leu agc Ser 195 aag Lys t ac Tyr aac Asn ccg Pr o gag G u 275 ggc G y gag Gu ttg Leu gcg Al a ggc G y 100 gcc Al a gcg Al a at t SIe ggt Gy cgc Ar g 180 tgg Tr p gtt Val cgt Ar g gt c Val aat Asn 260 ctt Leu ggt G y 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 agc ggg ctt atc gac gcc gtc Ser y Leu Ile Asp Ala Val 305 Page 42 ggc cgc gaa aaa atc cgc gca Sy Arg u Lys lie Arg Al a 310 315 ttt tca gcc atg gga att cca Phe Ser Ala Met y I e Pro 330 agt ttc gcg cat aaa cgc gac Ser Phe Ala His Lys Arg Asp 345 gat gtg gca gca att ttg aag Asp Val Ala Ala lle Leu Lys 360 cggggttatc gtg <210> 32 <211> 369 <212> PRT seql i st cor r ect ed. t xt aaa ttc ggc tgg acc gat gtc tca cgt Lys Phe y Trp Thr Asp Val Ser Arg 320 325 gcc cta aac ttt ggc gct ggt gat cca Ala Leu Asn Phe Gy Ala Gy Asp Pro 335 340 gag cag tgc cca gtg gag caa atc acg Su n Cys Pro Val u Gn Ile Thr 350 355 cag tac ctg agc gag taaccgcatt G n Tyr Leu Ser G u 365 1075 1123 1171 1217 1230 <213> Corynebacter i um gl ut ai cum <400> 32 Val Asn Ser 1 Val Leu Thr Lys G n Ile Pro G y Val Asn Arg G y Val Pro lle Tyr G y Cys Hi s Thr Phe 115 Thr Leu Ile 130 Leu G y Hi s 145 Ala Leu Leu G y Asn Leu Al a Arg Ser 195 G u Leu 5 G n Ar g Al a Asp G u Val Leu Al a Al a Asp G y Thr 100 Al a Thr Al a Tyr li e Ar g G y G u 165 Arg Ile 180 Trp Leu Pr o Val SIle Ar g Ar g Leu Asp Al a Cys 135 G u Thr Val Asp Tyr 215 Leu SIle 25 Asp Asn Met Ser Lys 105 Ser G u Pr o Gy Al a 185 Al a Asp 10 Pr o Al a Asn Leu Ar g 90 Ser Thr Val G u Tr p 170 Hi s bet Leu Ser Leu Asn Al a Val Gy G u Al a Tr p 155 I le Gy Hi s Gy Ser Asn Leu Hi s Asp Al a Lys 125 Hi s Al a Al a Ar g Leu 205 Asp Gy Leu Al a SIle Gy Val 110 Hi s Leu Al a Gy Al a 190 Ser Pr o G n Asn Ar g Asp SIe Tyr Asp Asn Asp Cys 175 Hi s Pr o lie Ser Lys Val Ala Ala 210 Lys Ala Ala u Val Asn I e Asp y 220 Page 43 Le u 225 A a Ph e Thr G31y Le u 305 Thr G31y ValI O31u Tyr As n Pr o O31u 275 G31y As p ValI G31y O31n 355 Ou le 245 Ar g As p A a ValI Ar g 325 Pr o Thr Le u As p Le u O31n Pr o 295 Ar g Ser Ph e ValI seqI i st car r ect ed. t xt Asn I Ie Val Phe Cys (31u 235 Leu Ala Tr p I'b t Asn Leu 250 As n 03 u A a I I e 03 u Hi s 265 As p G31y IIe O31u Tr p Ala 280 285 O3 y Leu 03 y 03 n 03 n Val 300 O31u Lys I Ie Ar g Ala Lys 315 Ala 'b t G31y I Ie Pr o Ala 330 Ala Hi s Lys Ar g As p O31u 345 Al a A a I I e Leu Lys 03 n 360 365 G31y Ph e 255 ValI O31u Ser G31y As n 335 Cys Le u <210O> 33 <21 1> 1059 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1036) <223> RXA00044 <400> 33 at tacct cag cct tccaagc t gat gat gca t tact taaaa ttctcacccg cactcgttcc ctcaacccac aaggagcacc tt c Ph e agt Ser ggt G31y tt c Ph e c ac Hi s ggc G31y g at As p gt c ValI ac c Th r gca A a at c lIe g aa O31u gga G31y gcc A a cgc Ar g cca Pr o ag c Ser ct t Le u cag O31n gt t ValI 75 ccc Pr a ct c Le u tt c Ph e cgc Ar g ccc Pr a gt a ValI cgc Ar g gca A a 45 aaa Lys5 gt a ValI at g vb t aag Lys5 ct g Le u ct c Le u act Th r acc cca Thr Pr o 15 ct c gt t Leu Val ggc t cc O3 y Ser gca ct g A a Leu gct ggt A a 03 y 80 Page 44 act gcagaca ct tgaaaaac at g gct t cc gca act I'b t A a Se r A a Thr 1 ct c cac gcc gac ggc Leu Hi s A a Asp 03 y gac cac ct c at c aat Asp Hi s Leu Il e Asn t ca ggc gaa gcg gca Ser 03 y 03 u A a A a acc acc at c at c gag Thr Thr Ilie Ilie O31u gtc att gaa acc acc Val Ilie O31u Thr Thr act gct cgc Th r Al a Ar g gt g at t gag ct c gt g Val Ilie u Leu Val eqIi s g aa aGu g aa G31u g aa G31u cca Pr o cca Pr o 1 50 aag Ly s cgt Ar g g aa G31u cca Pr o ct c Le u 230 at c I Ie t cc Ser ct t Le u ag c Ser t ac Tyr 310 ct c Le u g aa G31u 120 tt t Ph e at g vb t t cc Ser g at As p ac c Thr 200 ct g Le u ct c Le u c ac Hi s t cc Ser at t I Ie 280 g aa G31u gct Al a gcc Al a c ac Hi s t ac Tyr tt g Le u ggc G31y 1 70 gga G y g at As p aat As n gga G31y tt c Ph e 250 gct Al a g aa (31u act Thr gca Al a cgc Ar g at c I Ie 140 ct g Le u g at As p act Thr gcc Al a g at As p 220 t gg Tr p at c I Ie tt g Le u aat As n cgc Ar g 300 cct Pr o aag Ly s 125 cca Pr o gcc Al a ggc G31y gag G u t ac Tyr 205 cct Pr o gca Al a gt c ValI ggc G31y gcg Al a 285 at t I Ie tt c Ph e 110 at c I Ie gt g ValI aag Ly s gca Al a c ag G n 1 90 ct t Le u gca Al a g aa 0Gu tt c Ph e ggt 0Gy 270 at g vb t c ac Hi s t Cal g at As p 95 t ac Tyr c ac Hi s t cg Ser g at As p at c I Ie 1 75 tt c Ph e gcg Al a gca Al a gct Al a gt g ValI 255 tt c Ph e gca Al a gcc Al a r ect ed.t gcc ct g Al a Leu ac c Thr gcc Al a gt g ValI ggc G31y 1 60 cgc Ar g aag Ly s ggt G31y t ac Tyr gct Al a 240 gga G31y aag Ly s gt t ValI at t I Ie cgc Ar g gcc Al a c ac Hi s 145 gt t ValI t ca Ser at c I Ie gcc Al a gca Al a 225 gct Al a g ac As p ac a Thr cct Pr a gt t ValI 305 xt gag gct ggt gcc G3 u Al a G3 y Al a 1 00 acc cac gat gt g Thr Hi s Asp Val 115 gct cca gag ct t Al a Pr o G31u Leu 130 t cc aac ct C aac Ser Asn Leu Asn ctt gca ggc acc Leu Al a G3 y Thr 1 ct g at c gaa gct Leu Il e G3 u Al a 1 ct C acc ggc agc Leu Thr G y Ser 1 gat gga gt t gt c Asp a31y Val Val 210 gct t ta gca aaa Al a Leu Al a Lys ttg cag aag cgc Leu a31n Lys Ar g 245 acc t cc cat at g Thr Ser Hi s I'b t 260 gca ct c gca cac Al a Leu Al a Hi s 275 cac cag agc ct c His a31n Ser Leu 290 gat gaa t tc ct g Asp a3 u Phe Leu 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 059 t aaggcccac acct cat gac t ga <210O> 34 <21 1> 312 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 34 I'b t Al aSer Ala Thr Phe Thr ay Val Ilie Pro Pro Val I'bt Thr Pro Page seql i st cor r ect ed. t xt Leu Asp Ser Thr Val Leu Ar g Al a Hi s 145 Val Ser I le Al a Al a 225 Al a Asp Thr Pr o Val 305 Al a Leu G u SIle G u Al a Hi s 115 Pr o Asn Al a SIle Thr 195 Gy Leu G n Ser Leu 275 G n G u Gy Ser Val Asp Asn Al a G u Thr Al a Val Leu Asn Thr 165 Al a Ser Val Lys Ar g 245 Met Hi s Leu Gy Leu Thr Al a Gy SIle Leu 135 Val Asp Asp Thr Gy 215 Cys Asn Gy Gy Asp 295 Val Asp Ar g Gy Val Val 105 G u Al a Leu Ser Al a 185 Val Gy Asp Leu Ser 265 I le G u Su Ser Leu Arg Lys G y Leu Phe Al a Leu Ala G n Arg Lys Leu Arg Val Pro Val Thr 75 Ile u Leu Val u 90 Ala Thr Ala Pro Phe 110 His Phe Arg Lys Ile 125 Tyr Asn lie Pro Val 140 Leu Thr Leu Ala Lys 155 G y Asn Asp G y Al a 170 G y Leu Thr G u G n 190 Asp Phe Ala Tyr Leu 205 Asn Val Asp Pro Ala 220 Sy Lys Trp Ala u 235 Phe His lie Val Phe 250 Al a G y Leu G y G y 270 G u Ser Asn Ala IVet 285 Thr Ala Arg Ile His 300 Leu Gy Al a Al a Asp Tyr Hi s Ser Asp l Ie 175 Phe Al a Al a Al a Val 255 Phe Al a Al a Val Ser Leu Gy Al a Thr Al a Val Gy 160 Ar g Lys Gy Tyr Al a 240 Gy Lys Val SIe Phe Leu Tyr Thr Ala 310 <210> <211> 867 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(844) Page 46 seqi i st car r ect ed. t xt <223> RXA00863 <400> aacggtcagt taggtatgga tatcagcacc ttctgaacgg gtttgaaaaa ctcttcgccc cacgaaaatg aaggagcata gtacgtctag actggtgggc atg gga atc aag gtt 11! l'bt 3y lIe Lys Val ggc G31y gt c Val gat Asp ttc Phe aac Asn ttg Leu ct g Leu t cc Ser cac Hi s gct Al a 150 cca Pr o gat Asp gag O31u gac Asp cgc Ar g 230 ct c Leu gag O31u ttg Leu act Thr at t l Ie cag O31n gca Al a cag O31n 120 ccc Pr o ggc G31y gcg Al a at c l Ie gt t Val 200 t at Tyr at t l Ie gcc Al a gac Asp ct t Leu aac Asn gcg Al a cgc Ar g aac Asn gcc Al a aag Lys gct Al a gag O31u 170 gt t Val ttt Phe cgc Ar g cag (31n 99c G31y ct g Leu gt a Val gt g Val gt t Val t gg Tr p gct Al a ttc Phe gat Asp 140 gca Al a gca Al a gca Al a acc Thr t ca Ser 220 cca Pr o cgt Ar g gag O31u gac Asp 45 at g lvb t gga G31y ct t Leu at c l Ie ttc Phe 125 gca Al a cgc Ar g ct t Leu gt c Val cag O31n 205 ttt Phe ggc (31y gt t Val ct t Leu aac Asn ggc G31y acc Thr gaa O31u t ct Ser 110 gaa O31u cct Pr o aaa Lys gag O31u cgc Ar g 190 ggt G31y gca Al a ct a Leu ggt G31y 15 gt t Val ggc G31y aac Asn acg Thr gga G31y 95 gcg Al a t ca Ser t ca Ser g aa O31u ggt G31y 175 at g lvb t cag O31n cca Pr a gt c Val caa O31n gca Al a gct Al a ct g Leu ggc O31y aaa Lys gt g Val gct Al a ggc O31y gca Al a 160 t cc Ser t cc Ser acc Thr ggt O31y gt a Val 240 at t l Ie at c l Ie gt t Val ttc Phe gat Asp aat Asn acc Thr gt t Val 130 gcg Al a at g lvb t ggc O31y at g lvb t acc Thr 210 ttg Leu ct t Leu gca Al a gt c Val gt t Val at c l Ie gct Al a ggt O31y 100 gt c Val gag O31u cac Hi s gca Al a agc Ser 180 gct Al a aag Lys ggt O31y cat Hi s Page 47 seqI i st car r ect ed. t xt ct a ggc ct g t aaaggct ca t t tcagcagc ggg Leu G3 y Leu <210O> <211 <21 2> <21 3> 36 248 PmT Cor ynebact er i urn gi ut ani curn <400> 36 Thr I Ie G31u I Ie 31u Val G3 u Phe Phe As p As p As n Leu Thr G3 u Val 130 Thr A a 145 31y Ib t Ar g G31y 31y Ib t Leu Thr 210 Val Leu 225 G3 y Leu <210O> 37 I e Lys Val ValI ValI cys As p ValI 115 le le As p A a ValI 1 95 ValI A a As p As p As n Ar g ValI Ph e Le u Th r a31n 1 65 ValI Hi s a31n ValI Ty r 245 a31y ValI L eu a31y Ala 10 ValI As n a31u Se r As p Asp Asp Leu Ser Leu 40 Phe Thr Thr Pro Asn 55 As n a3 y I I e Se r A a 70 Leu a31u a31n Val Ar g 90 Leu Ilie Ala Pr o Asn 1 05 Ser Lys a3 n A a A a 120 Hi s Hi s Pr o Asn Lys 135 A a a3 n a3 y IlIe A a 150 Pr o Asp Ala Thr a31u 1 70 Asp a31y Ilie Pr o Val 1 85 a31u a31n Val Ilie Phe 200 Asp Ser Tyr Asp Ar g 215 Ar g Asn Ilie Ala a31n 230 Leu a3 y Leu Ly s As p Le u A a ValI As p Ph e Ar g Le u A a 1 a31n Hi s a31y As n Hi s 235 a31y Le u ValI ValI ValI Tr p A a Ph e As p 140 A a A a A a Thr Ser 220 Pr o Ar g a31u As p vb t a31y Le u I Ie Ph e 125 A a Ar g Le u ValI a31n 205 Ph e a31y ValI Le u As n a31y Thr a31u Ser 110 a31u Pr o Ly s a31u Ar g 1 a31y A a Le u a31y ValI a31y As n Thr a31y A a Ser Ser a31u a31y 1 vb t a31n Pr o ValI <21 1> 873 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CIDS Page 48 seql i st cor r ect ed. t xt <222> (101)..(850) <223> RXA00864 <400> 37 acagcaccca ggcctagtcg taggacttga gcattaccta agcagcgggt ggaatttttt aaaaggagcg tttaaaggct ggcct gt aaa ggct cat t t c gtg gcc gaa caa gtt 11! Val Ala Gu n Val aaa Lys gat Asp ttt Phe act Thr act Thr tct Ser tct Ser gt g Val cac Hi s ct g Leu 150 cag G n aga Ar g at g Met gaa G u agc Ser gag G u ggt Gy t cc Ser ttg Leu t cc Ser ct g Leu act Thr 120 at g Met gaa G u cgt Ar g gt g Val gcc Al a 200 tta Leu gag G u t ca Ser gcc Al a gct Al a gag G u acc Thr cag G n at c SIle gag G u ctt Leu gca Al a 170 tct Ser gaa G u aag Lys tt g Leu act Thr tgc Cys gcg Al a cat Hi s 75 cat Hi s cgt Ar g gat Asp tct Ser ggc Gy 155 gct Al a gga Gy cat Hi s ct g Leu at a SIle gat Asp t ac Tyr t at Tyr gcc Al a gaa G u ttc Phe gaa G u cgg Ar g 140 gat Asp cgc Ar g aac Asn gca Al a cag G n 220 gcg Al a gtt Val gaa G u 45 ct g Leu aat Asn ttg Leu gt g Val gat Asp 125 ttc Phe gaa G u gct Al a ttc Phe gac Asp 205 gt a Val tgc Cys gag G u act Thr cgc Ar g gcc Al a gt c Val cac Hi s 110 ccg Pr o gct Al a ccg Pr o gt g Val cgc Ar g 190 gt c Val gca Al a agt Ser 15 ggc Sy ttt Phe cac Hi s acg Thr cga Ar g 95 agc Ser cag G n ttc Phe aat Asn ct g Leu 175 acc Thr gaa G u gcg Al a tct Ser gcg Al a gat Asp at c SIle at g Met 80 cac Hi s gga G y ttg Leu aat Asn gca Al a 160 ccc Pr o tgg Tr p at c SIle cca Pr o 1 ttt Phe gaa G u aag Lys at g Met t at Tyr cgc Ar g gaa G u cgt Ar g gag G u 145 ctt Leu aac Asn agg Ar g cgc Ar g act Thr 225 act Thr gca Al a ccg Pr o gaa G u at c SIle cat Hi s tcg Ser gaa G u 130 ct g Leu tta Leu gct Al a cat Hi s gaa G u 210 gtt Val cca Pr o ct c Leu aac Asn gt g Val cga Ar g ttt Phe gaa G u 115 ctt Leu ctt Leu agg Ar g aca Thr ttc Phe 195 gt a Val ttc Phe ccc Pr o gt c Val cct Pr o ggg Sy ggc G y t cc Ser 100 gt a Val ttc Phe aat Asn aaa Lys gag G u 180 at t SIle gcg Al a ggt Sy gct Al a gag G u cga Ar g cac Hi s att SIe ttc Phe gtg Val at g Met gcg Al a aag Lys 165 t cc Ser ggc Sy gta Val gat Asp ttt gag att gaa act ttg gca gac gga tcg caa atg gca aca agc ccg Phe u Ile u Thr Leu Ala Asp y Ser n IVet Ala Thr Ser Pro Page 49 seql i st cor r ect ed. t xt 230 235 240 tat gtc atg gac ttt taacgcaaag ctcacaccca cga Tyr Val IVet Asp Phe 250 <210> 38 <211> 250 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 38 Val 1 Phe G u Lys Met Tyr Ar g G u Ar g G u 145 Leu Asn Ar g Ar g Thr 225 ?bt Al a G u G n Thr Pro Pro Al a Leu Val Pro Asn Pr o Gu Val G y lie Arg y Hi s Phe Ser 100 Ser G u Val 115 G u Leu Phe 130 Leu Leu Asn Leu Arg Lys Al a Thr G u 180 His Phe Ile 195 Su Val Ala 210 Val Phe G y Al a Thr Ser Val Lys 5 Al a Asp G u Phe Arg Thr Hi s Thr 70 Ile Ser Phe Ser Val Val bet His Al a Leu 150 Lys G n 165 Ser Arg G y Met Val G u Asp Phe 230 Pro Tyr 245 Leu Val Al a Al a 55 Al a Ar g G n Pr o Al a 135 G u Al a SIle Ar g Cys 215 G u Val Val Tr p 25 Ar g Asn Leu Al a Ser 105 Leu Asp Lys G n Val 185 Ser Ar g a u Asp G u 10 Ser Al a Al a G u Thr 90 G n SIle G u Leu Al a 170 Ser G u Lys Thr Phe 250 Leu Thr Cys Al a Hi s Hi s Ar g Asp Ser Sy 155 Al a Sy Hi s Leu Leu 235 SIle Asp Tyr Tyr Al a G u Phe G u Ar g 140 Asp Ar g Asn Al a G n 220 Al a Al a Val G u Leu Asn Leu Val Asp 125 Phe G u Al a Phe Asp 205 Val Asp Cys G u Thr Ar g Al a Val Hi s 110 Pr o Al a Pr o Val Ar g 190 Val Al a Sy Ser Sy Phe Hi s Thr Ar g Ser G n Phe Asn Leu 175 Thr G u Al a Ser <210> 39 <211> 608 <212> DNA <213> Corynebacter i um gl ut ai cum <220> Page seql i st cor r ect ed. t xt <221> CDS <222> (69)..(608) <223> RXA02843 <400> 39 cccat t gcgc ggaggtcgca cccct t ccga ct t gaactga taggccgata gaaat t at t c tggacgtc atg act act gct tcc gca acc gga att gca aca ctg acc tcc 110 Met Thr Thr Al a Ser Al a Thr G y I le Al a Thr Leu Thr Ser 1 5 acc ggc gac gtc ctg gac gtg tgg tat cca gaa atc ggg tcc acc gac 158 Thr y Asp Val Leu Asp Val Trp Tyr Pro G u lie Gy Ser Thr Asp 20 25 cag tcc gcg ctc aca cct cta gaa ggc gtc gat gaa gat cga aac gtc 206 G n Ser Ala Leu Thr Pro Leu G u Gy Val Asp G u Asp Arg Asn Val 40 acc cgc aaa atc gtg acg aca act atc gac acc gac gca gcc ccc acc 254 Thr Arg Lys lie Val Thr Thr Thr Ile Asp Thr Asp Ala Ala Pro Thr 55 gac acc tac gat gca tgg ctg cgc ctt cac ctc ctc tcc cac cgc gtt 302 Asp Thr Tyr Asp Ala Trp Leu Arg Leu His Leu Leu Ser His Arg Val 70 ttc cgc cct cac acc atc aac cta gac ggc att ttc ggc ctc ctc aac 350 Phe Arg Pro His Thr Ile Asn Leu Asp Gy lie Phe Gy Leu Leu Asn 85 aat gtc gtg tgg acc aac ttc gga ccg tgc gca gtt gac ggt ttc gca 398 Asn Val Val Trp Thr Asn Phe G y Pro Cys Ala Val Asp G y Phe Ala 100 105 110 ctc acc cgc gcg cgc ctg tca cgc cga ggc caa gtt acg gtt tat agc 446 Leu Thr Arg Ala Arg Leu Ser Arg Arg Gy G n Val Thr Val Tyr Ser 115 120 125 gtc gac aag ttc cca cgc atg gtc gac tat gtg gtt ccc tcg ggc gtg 494 Val Asp Lys Phe Pro Arg Iet Val Asp Tyr Val Val Pro Ser G y Val 130 135 140 cgc atc ggt gac gcc gac cgc gtc cga ctt ggc gcg tac ctg gca gat 542 Arg Ile Gy Asp Ala Asp Arg Val Arg Leu G y Ala Tyr Leu Ala Asp 145 150 155 ggc acc acc gtg atg cat gag ggc ttc gtg aac ttc aac gct ggc acg 590 G y Thr Thr Val IVet Hi s G u G y Phe Val Asn Phe Asn Al a G y Thr 160 165 170 ctc ggc gct tcc atg gtt 608 Leu G y Al a Ser Net Val 175 180 <210> <211> 180 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> bet Thr Thr Al a Ser Al a Thr G y I le Al a Thr Leu Thr Ser Thr G y 1 5 10 Asp Val Leu Asp Val Trp Tyr Pro u Ile y Ser Thr Asp G n Ser 25 Page 51 Al a Lys Tyr Pr o Val Ar g Lys Gy 145 Thr Al a Thr Val Al a Thr Thr Ar g 115 Pr o Al a Met Met Leu Thr Leu Asn Phe Ser bet Ar g G u 165 Gy I e Leu Asp Pr o Ar g Asp 135 Ar g Phe seql i st cor r ect ed. t xt Val Asp u Asp Arg Asn 40 Asp Thr Asp Ala Ala Pro His Leu Leu Ser His Arg G y I e Phe G y Leu Leu 90 Cys Ala Val Asp y Phe 105 Sy G n Val Thr Val Tyr 120 125 Tyr Val Val Pro Ser y 140 Leu y Ala Tyr Leu Ala 155 Val Asn Phe Asn Ala y 170 Thr Asp Phe Asn Leu Val Ar g Gy Leu 175 <210> 41 <211> 1143 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1120) <223> RXN00355 <400> 41 aatagatcag cgcatccgtg gtggaaccaa aaggctcaac ggtcctgatg aaagagatgt ccctgaatca tcatctaagt ct c Leu acc Thr gt c Val ttc Phe gcc Al a cag G n at c SIle aag Lys cgc Ar g gt g Val agt Ser gt a Val at t SIle gcc Al a aag Lys gcc Al a gct Al a gcc Al a acc Thr cac Hi s 75 acc Thr at c l Ie aag Lys ct c Leu gcc Al a aca Thr gt g Val cag G n 45 gac Asp gac Asp at t SIle ggc Gy ccc Pr o aca Thr gac Asp ttg gag Leu G u 15 tac gga Tyr G y gac atg Asp IVet aag acg Lys Thr gtg gac Val Asp 80 Page 52 aatacgaaac gttcgctttc atg cat ctc ggt aag 115 IVet His Leu G y Lys 1 gat tac aag aac atg 163 Asp Tyr Lys Asn IVet aac ctg gga cgc agc 211 Asn Leu Gy Arg Ser gac ctt gta gga atc 259 Asp Leu Val Gy Ile cca gtc ttt gat gtc 307 Pro Val Phe Asp Val gtg ctg ttc ctg tgc 355 Val Leu Phe Leu Cys atg ggc tcc gcc 'e t y Ser Ala acc Thr gac atc cc Asp Ile Pr seql i st cor r ect ed. t xt t gag cag gca cca aa o Gu Gn Ala Pro Lv g ttc s Phe gcg cag Al a n 100 w- w ,j ttc gcc tgc Phe Al a Cys cac cgc cag His Arg G n 120 gta gac acc tac Val Asp Thr Tyr aac cac cgc gac Asn His Arg Asp at c cca cgc I e Pro Arg 115 aac gtt gca Asn Val Ala gtc atg aac gaa Val Ibet Asn G u gcc Al a 125 gcc acc gca gcc Ala Thr Ala Ala ctg gt c Leu Val 135 tct acc ggc tgg Ser Thr Gy Trp gat Asp 140 cca gga atg ttc Pro Gy IVet Phe atc aac cgc gtc lie Asn Arg Val t ac Tyr 150 gca gcg gca gtc Al a Al a Al a Val tta Leu 155 gcc gag cac cag Ala G u His G n cag G n 160 cac acc ttc tgg His Thr Phe Trp cca ggt ttg tca Pro y Leu Ser cag G n 170 ggc cac tcc gat G y His Ser Asp ttg cga cgc atc Leu Arg Arg lle cct ggc Pro G y 180 gtt caa aag Val G n Lys aag gcc cgc Lys Ala Ar g 200 gtc cag tac acc Val G n Tyr Thr ct c Leu 190 cca tcc gaa gac Pro Ser G u Asp gcc ctg gaa Ala Leu G u 195 caa acc cac G n Thr His cgc ggc gaa gcc Arg G y G u Ala gac ctt acc gga Asp Leu Thr G y aag Lys 210 aag cgc Lys Arg 215 caa tgc ttc gtg G n Cys Phe Val gtt Val 220 gcc gac gcg gcc Al a Asp Al a Al a cac gag cgc atc His u Arg lle gaa G u 230 aac gac atc cgc Asn Asp lie Arg atg cct gat tac IVet Pro Asp Tyr ttc Phe 240 gtt ggc tac gaa Val y Tyr G u gaa gtc aac ttc G u Val Asn Phe at c Ile 250 gac gaa gca acc Asp G u Al a Thr gac tcc gag cac Asp Ser G u Hi s acc ggc Thr G y 260 atg cca cac bet Pro His aac cac acc Asn His Thr 280 ggc cac gtg att G y His Val lie acc Thr 270 acc ggc gac acc Thr G y Asp Thr ggt ggc ttc G y G y Phe 275 cca gat ttc Pro Asp Phe gtg gaa tac atc Val u Tyr lle aag ctg gac cga Lys Leu Asp Arg aac Asn 290 acc gct Thr Ala 295 t cc tca cag at c Ser Ser G n Ile gct Al a 300 ttc ggt cgc gca Phe G y Arg Ala gct Al a 305 cac cgc atg aag His Arg IVet Lys cag G n 310 cag ggc caa agc Sn G y G n Ser gga 31y 315 gct ttc acc gtc Al a Phe Thr Val ct c Leu 320 gaa gtt gct cca G u Val Ala Pro 1027 1075 1120 1143 ctg ctc tcc cca Leu Leu Ser Pro gag 33u 330 aac ttg gac gat Asn Leu Asp Asp atc gca cgc gac lie Ala Arg Asp gtc Val 340 taatttagct cgaggggcaa gga <210> 42 Page 53 seql i st cor r ect ed. t xt <211> 340 <212> PRT <213> Corynebact eri um gl ut arr cum <400> 42 Nbt His Leu G y Lys Leu Asp G n Asp Ser Al a Thr Thr II e Leu G u 1 5 10 Asp Tyr Lys Asn IVet Thr Asn Ile Arg Val Ala I e Val y Tyr y 25 Asn Leu y Arg Ser Val G u Lys Leu lie Ala Lys G n Pro Asp IVet 40 Asp Leu Val y Ile Phe Ser Arg Arg Ala Thr Leu Asp Thr Lys Thr 55 Pro Val Phe Asp Val Ala Asp Val Asp Lys His Ala Asp Asp Val Asp 70 75 Val Leu Phe Leu Cys IV t G y Ser Al a Thr Asp Ile Pro G u n Ala 90 Pro Lys Phe Ala G n Phe Ala Cys Thr Val Asp Thr Tyr Asp Asn His 100 105 110 Arg Asp lie Pro Arg His Arg G n Val IVet Asn G u Ala Ala Thr Ala 115 120 125 Al a G y Asn Val Al a Leu Val Ser Thr G y Trp Asp Pro G y IVt Phe 130 135 140 Ser lie Asn Arg Val Tyr Ala Ala Ala Val Leu Ala G u His n G n 145 150 155 160 His Thr Phe Trp G y Pro G y Leu Ser G n y His Ser Asp Ala Leu 165 170 175 Arg Arg Ile Pro y Val G n Lys Ala Val G n Tyr Thr Leu Pro Ser 180 185 190 G u Asp Al a Leu G u Lys Al a Arg Arg G y G u Al a G y Asp Leu Thr 195 200 205 G y Lys G n Thr His Lys Arg G n Cys Phe Val Val Ala Asp Ala Ala 210 215 220 Asp His u Arg lie G u Asn Asp lie Arg Thr IVet Pro Asp Tyr Phe 225 230 235 240 Val y Tyr G u Val G u Val Asn Phe lie Asp G u Ala Thr Phe Asp 245 250 255 Ser G u His Thr G y MIt Pro His G y G y His Val lie Thr Thr G y 260 265 270 Asp Thr G y G y Phe Asn Hi s Thr Val G u Tyr IIe Leu Lys Leu Asp 275 280 285 Arg Asn Pro Asp Phe Thr Ala Ser Ser n Ile Ala Phe y Arg Ala 290 295 300 Al a Hi s Arg MVt Lys G n G n G y G n Ser G y Al a Phe Thr Val Leu 305 310 315 320 G u Val Ala Pro Tyr Leu Leu Ser Pro G u Asn Leu Asp Asp Leu Ile 325 330 335 Page 54 seql i st cor r ect ed. t xt Ala Arg Asp Val 340 <210> 43 <211> 958 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(958) <223> FRXA00352 <400> 43 aatagatcag cgcatccgtg gtggaaccaa aaggctcaac ggtcctgatg aaagagatgt ccctgaatca tcatctaagt aatacgaaac gt t cgctttc ct c Leu acc Thr gt c Val ttc Phe gcc Al a at g Met ttc Phe cac Hi s ct g Leu t ac Tyr 150 cca Pr o gtt Val cag G n at c SIle aag Lys cgc Ar g gt g Val t cc Ser tgc Cys cag G n 120 tct Ser gcg Al a ttg Leu aag Lys agt Ser gt a Val at t SIle gcc Al a aag Lys acc Thr gt a Val at g Met ggc G y gt c Val cag G n 170 gt c Val gcc Al a gct Al a gcc Al a acc Thr cac Hi s 75 gac Asp gac Asp aac Asn tgg Tr p tta Leu 155 ggc G y cag G n acc Thr at c l Ie aag Lys ct c Leu gcc Al a at c SIle acc Thr gaa gat Asp 140 gcc Al a cac Hi s t ac Tyr aca Thr gt g Val cag G n 45 gac Asp gac Asp cct Pr o t ac Tyr gcc Al a 125 cca Pr o gag G u t cc Ser acc Thr at t SIle ggc Gy ccc Pr o aca Thr gac Asp gag G u gac Asp 110 gcc Al a gga G y cac Hi s gat Asp ct c Leu 190 ttg Leu 15 t ac Tyr gac Asp aag Lys gt g Val cag G n 95 aac Asn acc Thr at g Met cag G n gct Al a 175 cca Pr o gag G u gga G y at g Met acg Thr gac Asp gca Al a cac Hi s gca Al a ttc Phe cag G n 160 ttg Leu t cc Ser at g cat lMt His 1 gat tac Asp Tyr aac ct c Asn Let gac ctt Asp LeL cca gt c Pro Val gtg ct g Val LeL cca aag Pro Lys cgc gac Arg Asp gcc ggc Al a G y 13C t cc at c Ser Ile 145 cac acc Hi s Thr cga cgc Arg Arc gaa gac G u Asp aag Lys gga G y gt a Val ttt Phe ttc Phe ttc Phe at c SIle 115 aac Asn aac Asn ttc Phe at c SIle aac Asn cgc Ar g gga G y gat Asp ctg Leu gcg Al a 100 cca Pr o gtt Val cgc Ar g tgg Tr p cct Pr o 180 ct c ggt aag Leu G y Lys gcc ctg gaa Ala Leu G u 195 Page aag Ly s aag Ly s g aa O31u 230 g aa O31u at g vb t aac As n cgc Ar g 200 c aa O31n g ac As p aac As n c ac Hi s ac c Thr 280 ggC O31y tt c Ph e cgc Ar g at c I Ie 250 ggc O31y g aa O31u gaa O31u gt g ValI acc Thr 235 gac As p cac Hi s t ac Tyr gcc A a gt t ValI 220 at g vb t g aa O31u gt g ValI at c I Ie seql i st car r ect ed. t xt ggc gac ctt acc gga aag 03 y Asp Leu Thr 03 y Lys 205 210 gcc gac gcg gcc gat cac A a As p A a A a Asp Hi s 225 cct gat tac t tc gt t ggc Pr o Asp Tyr Phe Val O31y 240 gca acc ttc gac tcc gag A a Thr Phe Asp Ser 03 u 255 att acc acc ggc gac acc Il e Thr Thr 03 y Asp Thr 270 ct C aag Leu Lys 285 C aa O31n gag O31u t ac Tyr C ac Hi s ggt O31y 275 ac C Thr cgc Ar g g aa O31u ac C Thr 260 ggc O31y <210O> 44 <21 1> 286 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 44 I'bt Hi s Leu O31y Lys Leu Asp O31n Asp As p As n As p Pr o ValI Pr o Ar g A a Ser 145 Hi s Ly s O31y ValI Ph e Ph e Ph e I Ie 115 As n As n Ph e vb t Ser I Ie ValI cys O31n Ar g A a ValI O31y 1 65 As n O31u Ser As p O31y A a Ar g ValI 135 A a O31y I Ie Ly s 40 Ar g ValI Ser cys O31n 120 Ser A a Le u Ar g Le u Ar g As p A a Th r 1 ValI Th r A a Ser Ser 10 ValI I Ie A a Ly s Th r 90 ValI vb t O31y ValI O31n 1 70 A a A a A a Th r Hi s 75 As p As p As n Tr p Le u 1 55 O31y Th r I Ie Ly s Le u A a I Ie Th r O31u As p 140 A a Hi s Th r ValI O31n As p As p Pr o Ty r A a 125 Pr o O31u Ser Le u Ty r As p Ly s ValI O31n As n Th r vb t O31n A a 1 O31u O31y vb t Th r As p A a Hi s A a Ph e O31n 1 Le u Arg Arg Ilie Pro Oy Val On Lys Ala Val On Tyr Thr Leu Pro Ser Page 56 (31u G31y As p 225 ValI Ser As p A a 1 95 O31n (31u Tyr Hi s G31y 275 (31u Hi s I Ie ValI 245 G31y Ph e A a Ar g 215 As n ValI Pr o Hi s seql i st car r ect ed. t xt Ar g Ar g 31y O31u Ala G31y 200 205 O31n Cys Phe Val Val Ala 220 As p I Ie Ar g Thr l'b t Pr o 235 As n Phe I I e As p 03 u A a 250 His G31y G31y Hi s Val I Ie 265 Thr Val O31u Ty r I Ie Leu 280 285 Leu Thr A a A a Ty r Phe 240 Phe As p 255 Thr 03 y <210O> <211 <212> <213> <220> <221 <222> <223> 1400 DNA Cor ynebact er i urn gI ut anm cumn ODS (1)..(1377) RXA0 0972 <400> cct gca cct Pr o Ala Pro aca gt t gaa Thr Val O31u gcc gt g cgc Ala Val Ar g cct gac ct c Pro Asp Leu gac gat tt c Asp Asp Phe cca ggc aat Pro G31y Asn gca cgt t gg Ala Ar g Tr p aac gaa ctg Asn 03 u Leu 115 acc gcg cac Thr A a Hi s 130 t gg Tr p tt c Ph e g aa O31u g aa O31u t cc Ser c ac Hi s g at As p at t I Ie aac As n cgt Ar g aat As n gac As p gaa O31u cgc Ar g 70 t ac Tyr gaa O31u gcc A a aac As n tt c Ph e g aa O31u G31y t ac Tyr 55 t gt Cys gca A a gag O31u ct g Le u aaa Ly s 135 ac c Thr CCC Pr o gt c ValI ac c Thr g ac As p aaa Ly s ct g Le u 1 05 gct A a gt a ValI gga G31y 10 gca A a ac c Thr cca Pr o at g vb t gcg A a 90 gca A a ggt G31y gag O31u g aa O31u c ac Hi s gt c ValI ct g Le u gct A a tt c Ph e ct g Le u tt c Ph e tt c Ph e g at As p gt a ValI gct A a tt c Ph e ac c Thr ct g Le u g ac As p CCC Pr a ct g Le u 140 gt a ValI t gg Tr p ggt G31y gt a ValI gca A a ac c Thr at t I Ie gcc A a 125 cgc Ar g ac a Thr cca Pr a gt g ValI gt c ValI tt c Ph e aag Ly s gca A a 110 ag c Ser gcg A a at g vb t cgc Ar g cct Pr a g ac As p ggt G31y ac c Thr t cc Ser cgt Ar g tt g Le u caa aac ggt gtg gga cac gtg gtg On Asn Gy Val Gy His Val Val ct g gac t cc gca cag gaa ct a gaa Leu Asp Ser A a 03 n 03 u Leu 03 u Page 57 seql i st cor r ect ed. t xt 145 150 155 160 ctg ttg gat tac gtt gcc gct ggt gaa ggc aag att cag gac gtg ttg 528 Leu Leu Asp Tyr Val Al a Ala Gy G u y Lys I e G n Asp Val Leu 165 170 175 atc cgc gta aag cca ggc atc gaa gca cac acc cac gag ttc atc gcc 576 IIe Arg Val Lys Pro G y IIe G u Ala His Thr His G u Phe IIe Ala 180 185 190 act agc cac gaa gac cag aag ttc gga ttc tcc ctg gca tcc ggt tcc 624 Thr Ser His G u Asp G n Lys Phe G y Phe Ser Leu Ala Ser G y Ser 195 200 205 gca ttc gaa gca gca aaa gcc gcc aac aac gca gaa aac ctg aac ctg 672 Ala Phe G u Ala Ala Lys Ala Ala Asn Asn Ala G u Asn Leu Asn Leu 210 215 220 gtt ggc ctg cac tgc cac gtt ggt tcc cag gtg ttc gac gcc gaa ggc 720 Val G y Leu His Cys His Val G y Ser G n Val Phe Asp Ala G u G y 225 230 235 240 ttc aag ctg gca gca gaa cgc gtg ttg ggc ct g tac tca cag at ccac 768 Phe Lys Leu Ala Ala G u Arg Val Leu G y Leu Tyr Ser G n lie His 245 250 255 agc gaa ctg ggc gtt gcc ctt cct gaa ctg gat ctc ggt ggc gga tac 816 Ser G u Leu y Val Al a Leu Pro G u Leu Asp Leu y y y Tyr 260 265 270 ggc att gcc tat acc gca gct gaa gaa cca ctc aac gtc gca gaa gtt 864 Sy Ile Ala Tyr Thr Al a Ala G u G u Pro Leu Asn Val Ala G u Val 275 280 285 gcc tcc gac ctg ctc acc gca gtc gga aaa atg gca gcg gaa cta ggc 912 Al a Ser Asp Leu Leu Thr Al a Val G y Lys IV t Al a Al a G u Leu G y 290 295 300 atc gac gca cca acc gtg ctt gtt gag ccc ggc cgc gct atc gca ggc 960 Ile Asp Ala Pro Thr Val Leu Val u Pro y Arg Ala Ile Ala y 305 310 315 320 ccc tcc acc gtg acc atc tac gaa gtc ggc acc acc aaa gac gtc cac 1008 Pro Ser Thr Val Thr IIe Tyr G u Val G y Thr Thr Lys Asp Val His 325 330 335 gta gac gac gac aaa acc cgc cgt tac atc gcc gtg gac gga ggc atg 1056 Val Asp Asp Asp Lys Thr Arg Arg Tyr I e Al a Val Asp G y G y Iet 340 345 350 tcc gac aac atc cgc cca gca ctc tac ggg tcc gaa tac gac gcc cgc 1104 Ser Asp Asn lie Arg Pro Ala Leu Tyr y Ser G u Tyr Asp Ala Arg 355 360 365 gta gta tcc cgc ttc gcc gaa gga gac cca gta agc acc cgc atc gtg 1152 Val Val Ser Arg Phe Al a G u y Asp Pro Val Ser Thr Arg lie Val 370 375 380 ggc tcc cac tgc gaa tcc ggc gat atc ctg atc aac gat gaa atc tac 1200 G y Ser His Cys u Ser G y Asp IIe Leu IIe Asn Asp G u IIe Tyr 385 390 395 400 cca tct gac atc acc agc ggc gac ttc ctt gca ctc gca gcc acc ggc 1248 Pro Ser Asp lie Thr Ser y Asp Phe Leu Ala Leu Ala Ala Thr y 405 410 415 gca tac tgc tac gcc atg agc tcc cgc tac aac gcc ttc aca cgg ccc 1296 Ala Tyr Cys Tyr Ala IV t Ser Ser Arg Tyr Asn Ala Phe Thr Arg Pro Page 58 seqI i st car r ect ed. t xt 420 425 430 gcc gtc gtg tcc gtc cgc gct ggc agc tcc cgc ctc atg ctg cgc cgc 1344 Al a Val Val Ser ValI Ar g Al a 03 y Ser Ser Ar g Leu I'bt Leu Ar g Ar g 435 440 445 gaa acg ct c gac gac at c ct c t ca ct a gag gca t aacgct t tt cgacgcct ga 1397 O31u Thr Leu Asp Asp Ilie Leu Ser Leu O31u Al a 450 455 ccc 1400 <210> 46 <211> 459 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn Pr a Thr Al a Pr a As p Pr a Al a As n Thr O31n 145 Le u I Ie Thr Al a ValI 225 Ph e O> 46 Al a Pr o (31y Val 31u As n Val Ar g G n Asp Leu Al a Asp Phe Arg G31y Asn Val Ar g Tr p Val 1 00 O3 u Leu (3 y 115 Al a Hi s (3 y 130 Asn G31y Val Leu Asp Tyr Ar g Val Lys 1 80 Ser Hi s (31u 1 95 Phe 03 u Al a 210 O3 y Leu Hi s Lys Leu Al a Tr p Ph e G u 0Ou Ser Hi s As p I Ie As n 0Gy ValI 1 65 Pr a As p Al a cys Al a 245 Ar g Phe Ar g Thr O31u G y Tyr 55 cys Al a O31u Le u Lys5 135 ValI Al a I Ie Lys5 Al a 215 ValI Ar g Le u ValI 40 G31y Ar g Ser G31y Al a 120 G31y ValI G31y O31u Ph e 200 Al a G31y ValI Pr a 25 ValI Thr As p Lys5 Le u 1 05 Al a ValI Le u O31u Al a 1 85 G31y As n Ser Le u G31y 10 Al a Thr Pr a vb t Al a 90 Al a G31y O31u As p G31y 1 70 Hi s Ph e As n O31n G31y 250 O31u Hi s ValI Le u Al a 75 Ph e Le u Ph e Ph e Ser 1 55 Lys5 Thr Ser Al a ValI 235 Le u As p ValI Al a Ph e Thr Le u As p Pr a Le u 140 Al a I Ie Hi s Le u O31u 220 Ph e Tyr ValI Tr p G y ValI Al a Thr I Ie Al a 125 Ar g O n O n 0Ou Al a 205 As n As p Ser Thr Pr a ValI ValI Ph e Ly s Al a 110 Ser Al a O31u As p Ph e 1 Ser Le u Al a O31n Nibt Ar g Pr a As p O31y Thr Ser Ar g Le u Le u ValI 1 li1e O31y As n Ou le 255 Al a As n Le u O31u O31y li1e I Ie I Ie ValI O31u 1 Le u Al a Se r Le u O31y 240 Hi s Ser O31u Leu O31y Val Al a Leu Pro O31u Leu Asp Leu O31y O31y O31y Tyr Page 59 seql i st cor r ect ed. t xt 265 Gy Al a SIle 305 Pr o Val Ser Val Gy 385 Pr o Al a Al a G u Al a 275 Asp Al a Thr Asp Asn 355 Ser Hi s Asp Cys Val 435 Leu Tyr Thr Ala Ala G u Pro Leu Asn Leu Thr Thr 325 Lys Ar g Phe G u Thr 405 Al a Val Asp Al a 295 Leu Tyr Ar g Al a G u 375 Gy Gy Ser Al a Leu 455 Gy G u Val Tyr 345 Tyr Asp SIle Phe Ar g 425 Ser Leu Met Gy 315 Thr Al a Ser Val SIle 395 Al a Asn Ar g Al a Al a 300 Ar g Thr Val G u Ser 380 Asn Leu Al a Leu Val 285 Al a Al a Lys Asp Tyr 365 Thr Asp Al a Phe bet 445 Al a G u l e Asp Gy 350 Asp Ar g G u Al a Thr 430 Leu G u Leu Al a Val 335 Gy Al a SIe SIe Thr 415 Ar g Ar g <210> 47 <211> 2121 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(2098) <223> RXA02653 <400> 47 agacagagtg ttagtgcgtg gggcagctct cactttcatc gacatcactc gagtatgctc accggccgta ttcattccaa taacccgcac agggaaacta atg ata ccg aag ccc 115 IVet Ile Pro Lys Pro 1 gac gtg acc gac tta tat tta gag gac ctc tta aat gag ggt tcg gaa 163 Asp Val Thr Asp Leu Tyr Leu G u Asp Leu Leu Asn G u y Ser G u 15 aag att cgg tcc gcc aag gat ctt tcc gaa ctt agg aca gtt cta aaa 211 Lys lie Arg Ser Ala Lys Asp Leu Ser G u Leu Arg Thr Val Leu Lys 30 gag gtt tcc tcc caa att cag gaa cga gct ggg aaa aaa gat gaa gaa 259 Su Val Ser Ser Gn Ile Gn Gu Arg Ala y Lys Lys Asp Gu u 45 Page tgg gga atg ggg gcc act tgg Trp Gy lebt Gy Ala Thr Trp seql i st cor r ect ed. t xt cgg gag ctg tac ccc agc atc gtg gaa Arg G u Leu Tyr Pro Ser lie Val G u cgc Ar g gcc Al a gaa G u ct g Leu at a SIle ggc Gy 150 cct Pr o caa G n ggt Gy gct Al a ggt Gy 230 act Thr cct Pr o ttg Leu gcc Al a acc Thr 310 t cc Ser gaa Gu ct c Leu cgt Ar g 120 gt g Val ttg Leu act Thr cat Hi s gac Asp 200 cga Ar g cca Pr o gat Asp aat Asn gac Asp 280 aaa Lys cat Hi s gaa G u gaa Gu gaa Gu cgg Ar g at a SIle cac Hi s ggg Gy 170 gct Al a ttg Leu at c SIle ctt Leu gcc Al a 250 ct c Leu ggc Gy aat Asn gt a Val ggg Gy 75 aga Ar g ct c Leu gaa Gu gac Asp aat Asn 155 tta Leu gca Al a tcg Ser cgc Ar g ccg Pr o 235 aca Thr cat Hi s at t SIle aag Lys gat Asp 315 cgt Ar g tta Leu gt a Val cca Pr o gct Al a 140 ttg Leu gat Asp gt c Val cga Ar g gac Asp 220 gca Al a tgc Cys t ca Ser cag G n ggt Gy 300 gt a Val gac Asp gcc Al a aaa Lys ct g Leu 125 gtt Val gt a Val gt a Val gga Gy t ca Ser 205 gcg Al a cgg Ar g cgc Ar g ggt Gy at g Met 285 ctt Leu gcc Al a agc Ser ttc Phe t cc Ser 110 aca Thr ctt Leu aaa Lys gcg Al a cgg Ar g 190 tta Leu agc Ser ttg Leu cac Hi s tct Ser 270 ggg Gy acc Thr agt Ser ct a Leu ggc Gy 95 gga Gy gat Asp ccc Pr o gag Gu gct Al a 175 at g Met cat Hi s acg Thr gaa G u ct g Leu 255 at g Met gt g Val ttc Phe gaa G u at c I e cca Pr o gt g Val tta Leu ccg Pr o gga Gy 160 gac Asp acg Thr gac Asp t at Tyr ccc Pr o 240 at t SIle cct Pr o gat Asp gtt Val cga Ar g 320 gga Gy cca Pr o gt a Val gtt Val ccg Pr o 145 t at Tyr ggc Gy gaa G u at a SIle ccc Pr o 225 tgg Tr p gaa G u cgt Ar g act Thr gat Asp 305 gag G u ttt Phe t cc Ser gac Asp cgt Ar g 130 gga Gy gcc Al a acc Thr gat Asp at c SIle 210 gat Asp gcg Al a gaa G u aat Asn cga Ar g 290 gcc Al a tta Leu gat Asp gaa G u at c SIle 115 gaa G u gt a Val aga Ar g gtt Val gt g Val 195 ccg Pr o agg Ar g gaa G u ttc Phe at a SIle 275 at a SIle gtt Val tct Ser cac Hi s agt Ser 100 act Thr ctt Leu gt g Val at g Met caa G n 180 gtt Val aag Lys gt a Val aag Lys ggg Gy 260 aat Asn ttt Phe aaa Lys cag G n 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 61 seql i st cor r ect ed. t xt ctt aat cgt gga gtc cca gga gag Leu Asn Arg G y Val Pro G y G u 330 cgg atc att cta tcc Arg Ile Ile Leu Ser gca gct at c Al a Al a I e aaa Lys tct Ser gac Asp tta Leu 390 ctt Leu ct c Leu tgc Cys at t SIle gat Asp 470 gcc Al a ttc Phe aag Lys gag G u gtt Val 550 gct Al a gat Asp gac Asp gat Asp 360 gtt Val cca Pr o gag G u ggc Gy ct c Leu 440 ctt Leu at c SIle t cc Ser cag G n gt a Val 520 ggt Gy ttt Phe aac Asn ct g Leu ct a Leu cgt Ar g cga Ar g aga Ar g at a SIle 410 gct Al a gat Asp gga Gy t at Tyr acg Thr 490 cca Pr o cag G n agt Ser aaa Lys acg Thr 570 at c SIle tt g Leu gat Asp gtt Val ttt Phe 395 ccc Pr o gca Al a tct Ser ggg Gy caa G n 475 tgg Tr p gt g Val at g bet tta Leu acc Thr 555 cag G n act Thr gca Al a gaa G u gcg Al a 380 ggt Gy ggc Gy aaa Lys ct c Leu gt g Val 460 t cc Ser aaa Lys cgc Ar g ct c Leu ct a Leu 540 cga Ar g tgc Cys gac Asp tta Leu tta Leu 365 cct Pr o gag G u gt g Val gac Asp aga Ar g 445 cct Pr o gct Al a gac Asp ggt Gy at t SIle 525 gat Asp agt Ser cgg Ar g ggt Gy gcg Al a 350 gat Asp aga Ar g cgt Ar g gat Asp cgt Ar g 430 gaa G u at g Met aaa Lys gat Asp aat Asn 510 gac Asp ggg Gy gac Asp act Thr gat Asp 590 335 at c l Ie cgc Ar g gt a Val gct Al a at t SIle 415 gct Al a tgc Cys agc Ser tct Ser ccg Pr o 495 tgg Tr p cgg Ar g tgt Cys ggg Gy aca Thr 575 gt a Val gaa G u at t SIle gct Al a gca Al a 400 gt g Val ct g Leu ggg Gy t ac Tyr gcg Al a 480 tta Leu ttg Leu gga Gy ggc Gy ttg Leu 560 t cc Ser ggc Gy aat Asn tcg Ser cca Pr o 385 gac Asp ggt Gy gct Al a cat Hi s at t SIle 465 act Thr tct Ser aaa Lys ttg Leu gt c Val 545 cct Pr o gat Asp gag G u ggc Gy gct Al a 370 gat Asp tgg Tr p ctt Leu ct g Leu t cc Ser 450 gaa G u t ca Ser aat Asn gac Asp cgg Ar g 530 act Thr ct a Leu gat Asp gaa G u 340 at c SIle gtt Val gca Al a aat Asn gtt Val 420 gaa Gu cag G n gag G u ggg Gy t ac Tyr 500 ctt Leu cac Hi s gcc Al a gga Gy ct c Leu 580 gaa G u at c SIe ggt Gy gt c Val cgg Ar g 405 cac Hi s tgt Cys ttt Phe gaa G u t at Tyr 485 ccg Pr o tct Ser at a SIe gaa G u ct g Leu 565 at t SIe gca Al a 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 Page 62 t at Tyr cga Ar g cct Pr o 630 c aa O31n g at As p gt g ValI 600 cgc Ar g ac c Thr ccg Pr o g at As p gcc A a ccg Pr o gga G31y gcg A a 650 g at As p seqI i st car r ect ed. t xt t ac t gc at c gaa gat gag ct g at t tt a Ty r Cys I I e 03 u As p 03 u Leu I I e Leu 605 610 aga gga gtc aaa cca gga gat atc atc Ar g G31y Val Lys Pr o G31y Asp Ilie Ilie 620 625 tac t tc at g cat at c t tg gaa agt gca Tyr Phe I'b t Hi s Ilie Leu O31u Ser Ala 635 640 aaa aat gt a gt g t gg ccg gag ggg cag Lys Asn Val Val Tr p Pr o O31u 31y O31n 655 t aagacat aa ccatt cgct a at c cgc Ar g gga (31y t cg Ser tt a Le u 660 1 939 1 987 2035 2083 2121 <210O> 48 <21 1> 666 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 48 I'bt Ilie Pro Lys Pro Asp As n Ar g Ly s Pr o G31y Pr o ValI ValI Pr a 145 Tyr G31y O31u G31y ValI As p I Ie As p O31u I Ie 115 O31u ValI Ar g ValI ValI 1 95 O31u Ly s O31u O31u Le u Ph e Hi s O31u Pr a Ar g 1 65 G31y Le u ValI I Ie ValI G31y A a Ar g Tyr Hi s Thr 135 Thr G31y Ar g As n Ar g 215 As p Ser Ser G31y Tyr vb t O31n 1 05 G31y ValI ValI Ar g Le u 1 85 Thr Le u 10 A a O31n A a O31u O31u 90 O31u Ar g I Ie Hi s O31y 1 70 A a Le u Tyr Ly s I Ie Thr O31y Ar g Le u O31u As p As n 1 Le u A a Ser O31u Le u O31u Ar g As p A a Ly s Le u 125 ValI ValI ValI O31y Ser 205 As p Ser Ar g O31u Ser Ph e Ser 110 Thr Le u Ly s A a Ar g 1 Le u Le u O31u A a Le u Le u O31y O31y As p Pr a O31u A a 1 vb t Hi s Ilie Ilie Pro Lys Trp Ala 210 Arg Val lieArg A a Ser Thr Tyr Page 63 seqi i st car r ect ed. t xt Pro Asp Arg Val His Gy 225 230 Thr Pro Pro Leu Pro Ala Arg Leu Ou Tr p O31u Ar g Thr As p 305 O31u Le u As n Ser Pr o 385 As p G31y A a Hi s I Ie 465 Thr Ser Le u ValI 545 Pr o O31u Ph e I Ie 275 li1e ValI Ser A a ValI 355 Le u Pr o G31y Hi s O31n 435 Pr a Ser A a Thr ValI 515 Le u Le u ValI Le u 245 Ser O31u Ph e As p ValI 325 li1e I Ie G31y ValI Ar g 405 Hi s cys Ph e O31u Tyr 485 Pr a Ser I Ie O31u Le u 565 Thr Pr a Le u A a Thr 310 Le u Lys5 Ser As p Le u 390 Le u Le u Cy s I Ie As p 470 A a Ph e Lys5 O31u ValI 550 A a Ser ValI ValI Ar g 295 G31y As n Pr a ValI Ar g 375 Pr a Thr Hi s O31n As p 455 Tr p O31u Tyr O31y Pr a 535 A a vb t Pr a ValI 265 A a A a O31y O31y Ar g 345 Ser A a Thr ValI Tyr 425 ValI O31y Ar g Ph e Thr 505 A a Ar g ValI Ar g A a 250 Le u O31y As n ValI ValI 330 Le u Ar g Ar g Ar g I Ie 410 A a As p G31y Tyr Thr 490 Pr a O31n Ser Lys5 Thr 570 235 Thr Hi s I Ie Lys5 As p 315 Pr a Le u As p ValI Ph e 395 Pr a A a Ser G31y O31n 475 Tr p ValI vb t Le u Thr 555 O31n cys Ser O31n O31y 300 ValI O31y A a (31u A a 380 O31y O31y Ly s Le u ValI 460 Ser Ly s Ar g Le u Le u 540 Ar g Cys Ar g O31y vb t 285 Le u A a O31u Le u Le u 365 Pr a O31u ValI As p Ar g 445 Pr a A a As p O31y I Ie 525 As p Ser Ar g Le u 255 vb t ValI Ph e O31u I Ie 335 li1e Ar g ValI A a I Ie 415 A a cys Ser Ser Pr a 495 Tr p Ar g Cys O31y Th r 575 Asp Asp Phe Leu I Ie Asp Pro Leu Hi s I Ie Thr Asp O31y Asp Val O31y Page 64 O31u O31u I Ie 595 Leu Il e Leu 610 As p I I e I I e 625 03 u Ser Al a O31u (1y (1n seqI i st car r ect ed. t xt 580 585 590 O31u Ala Tyr Leu Val G31y Al a Tyr Cys I Ie O31u As p (31u 600 605 Ar g Ar g Ar g Ilie Ar g Phe Pr o Ar g G31y Val Lys Pr o (31y 615 620 G31y Ilie Pr o Asn Thr Al a G31y Tyr Phe N/b t Hi s I Ie Leu 630 635 640 Ser His O31n Ilie Pr o Leu Al a Lys Asn Val Val Tr p Pr o 645 650 655 Leu Asp Asp Il e Asp Al a Asp 660 665 <210O> 49 <21 1> 993 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> 101). (970) <223> RXA01393 <400> 49 caaaagcaga cct gt aat ga agat t tccat gat caccat c agt aaaat ga t t ggt t ct t a acat ggt t ta at at agct t c gt gacct at g gaagt act t a at g aac ccc at t caa 11! I'bt Asn Pro lieOn ct g Le u t cc Ser gct Al a gcc Al a at g vb t gct Al a t gg Tr p ct c Le u act Thr gcc Al a gag O31u gca Al a tt g Le u at c I Ie cct Pr o ct g Le u 120 ct c Le u t cc Ser c ac Hi s g aa O31u c aa O31n tt a Le u gt g ValI tt g Le u t ca Ser at t I Ie gt g ValI gcg Al a gca Al a 75 acc Thr t t c Ph e gaa (31u at c I Ie t cc Ser ggt G31y ggt G31y g aa (31u at c I Ie aac As n g at As p at t I Ie ccc Pr o cga Ar g 45 g aa (31u act Thr gcc Al a gag (31u g aa (31u 125 g at As p t cg Ser gt g ValI gt c ValI aaa Lys5 at c I Ie gt a ValI 110 gcg Al a g aa (31u 15 gcg Al a tt g Le u ct t Le u gcg Al a aac As n 95 gct Al a c ac Hi s ggc G31y gt g ValI gt a ValI gt g ValI c aa O31n 80 gca Al a t ct Ser ac a Thr 1 ag c Ser agt Ser t cg Ser c aa O31n 65 ct a Le u g at As p t gg Tr p tt a Le u tt c Ph e c ag O31n cgc Ar g 50 gca Al a t ct Ser t cg Ser ggt O31y t cc Ser 130 g aa O31u cgc Ar g 35 ac c Thr gcg Al a gga O31y ct a Le u gga O31y 115 tt g Le u ggc O31y 20 gt t ValI c aa O31n cgg Ar g cgc Ar g t cc Ser 1 00 gca Al a ct g Le u gcc Al a aaa ccg Pr o aaa ct t Le u ac a Th r ac g Th r cgg Ar g cgt gga gat gt t t ta gga gcg gt a acc cgt gaa gct aat ccc gt g gcg Page Arg G y Asp 135 gga t gt gaa G y Cys 3 u 150 acc ccc t ca Thr Pro Ser gct gcg at g Al a Al a Vbet cgt gac ct g Arg Asp Leu 200 tcc att gtc Ser Ile Val 215 ctt ggt tgg Leu G y Trp 230 gca gga gaa Al a G y G u tat tgg caa Tyr Trp G n gac gcc gtc Asp Al a Val 280 Val Leu G y Al a 140 seql i st cor r ect ed. t xt Val Thr Arg u Ala Asn 145 Pro Val Al a gt a Val cgg Ar g 170 gt c Val ggg Gy tcg Ser ctt Leu at c SIle 250 tgg Tr p gaa G u 155 gat Asp tta Leu cgc Ar g gcg Al a ctt Leu 235 ct c Leu cgc Ar g ctt Leu gcc Al a cgc Ar g gt c Val gaa G u 220 ccc Pr o ct c Leu ct g Leu gga G y t ac Tyr ttc Phe gat Asp 205 ggt Gy gaa G u gat Asp gaa G u acc Thr at g Met ggt Gy 190 ggt Gy ttt Phe acc Thr gag G u tct Ser 270 at g Met gtt Val 175 ccc Pr o cct Pr o ggt Gy caa G n at a SIle 255 aga Ar g cgc Ar g 160 gat Asp aaa Lys gt g Val gag G u gct Al a 240 ccc Pr o tct Ser cac Hi s ggg Gy gat Asp ggg Gy gca Al a 225 gct Al a at t SIle ct a Leu ttg Leu aaa Lys gt g Val cgc Ar g 210 at t l Ie ccc Pr o gac Asp gct Al a gcc Al a ct a Leu ctt Leu 195 agg Ar g cgc Ar g at g Met aca Thr aga Ar g 275 at t l Ie gat Asp 180 caa G n cgc Ar g cga Ar g ct a Leu ccg Pr o 260 ct c Leu gca Al a 165 tgg Tr p gac Asp gta Val ggc Gy aaa Lys 245 at g Met aca Thr gat gca gca atc gag gga ttg cgg cct Asp Ala Ala lle Gu Gy Leu Arg Pro 285 290 t agtt actt c tgaaaaggtt cag <210> <211> 290 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> IVet Asn Pro Ile G n Leu Asp Thr Leu 1 5 Ser Phe G u y Ala Ser Leu Ala Leu Ser G n Arg Val Lys Al a Leu G u His 40 Ser Arg Thr G n Pro Ala Lys Ala Thr G n Ala Ala Arg Lys IVet Val Leu Leu 70 Leu Ser y Arg Leu Al a G u lie Pro Asp Ser Leu Ser Thr Trp Phe Pro Pro 100 105 Leu Ser 10 Ser Ile His Val G u Al a G n Al a 75 Leu Thr 90 Val Phe Page 66 SIle Pr o Ar g G u Thr Al a G u G u Al a Leu Leu Al a Asn Al a Sy Val Val Val G n Al a Ser seqi i st car r ect ed. t xt Tr p 31y G31y 115 Ala Thr Leu Thr Leu Ar g Leu O31u Asp O31u Ala Hi s Thr 120 125 Le u A a 145 Hi s G31y As p G31y A a 225 A a I Ie Le u Ar g Le u Pr o A a Le u Le u 1 95 Ar g Ar g vb t Thr Ar g 275 Ar g A a A a 1 65 Tr p As p ValI G31y Ly s 245 vb t Thr Ar g G31y 150 Thr A a Ar g Ser Le u 230 A a Tyr As p G31y 135 cys Pr o A a As p I Ie 215 G31y (31y Tr p A a ValI ValI Le u Pr o 1 As p Pr o G31y ValI Ar g 265 ValI G31y O31u 1 As p Le u Ar g A a Le u 235 Le u Ar g A a A a 140 Le u A a Ar g ValI O31u 220 Pr a Le u Le u A a ValI G31y Tyr Ph e As p 205 G31y O31u As p Ou le 285 Ar g vb t ValI 1 Pr a Pr a G31y O31n I Ie 255 Ar g G31y <210O> 51 <21 1> 1626 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1603) <223> RXA00241 <400> 51 ggt ct ccagc ct t tctaaac aat tcat ct gcact tgatt a aagt t tagcg act tcgccgt acgt caact a cgt taaat ga g at As p at t I Ie at t I Ie at g gcg A a ac c Thr t cc Ser at c t ct Ser at c I Ie cct Pr a t gg caa O31n gcg A a caa O31n ct g gca A a at c I Ie at c lIe 45 gcc gcg A a at c lIe ggc G31y ggt gcc aca A a Thr 15 gga t cg O3 y Ser t ca gt c Ser Val gt g ggc Page 67 at t ggcccca agat t acgcg gt g aat act caa t ca Val Asn Thr 03 n Ser 1 agt cgt act gt a t ct Ser Ar g Thr Val Ser acc gt c ggc gcg gga Thr Val G31y Ala G31y gca ggt ccc ggc gcg Ala G31y Pr o G31y Ala at g t tg t cc gt a gcg seqI i st car r ect ed. t xt I'bt Leu Ilie Gy Trp Leu IlieAla Gy Val Gy [Vbt Leu 60 Se r ValI A a t tc Ph e gt c ValI t cc Ser gca A a c aa O31n ct g Le u 1 50 ac a Thr at c I Ie tt a Le u ggc G31y t cg Ser 230 ac c Thr t cg Ser g at As p gcc A a gt g ValI 310 tt c Ph e gca A a t gg Tr p tt a Le u 120 cat Hi s tt t Ph e gt c ValI gt t ValI gcg A a 200 at g vb t t at Tyr at t I Ie ag c Ser t cc Ser 280 tt g Le u t gg Tr p gt t ValI gcg A a t at Tyr tt c Ph e tt t Ph e gt t ValI ac c Thr 1 70 tt c Ph e g at As p t ac Tyr cgc Ar g tt t Ph e 250 ggt G31y gcg A a t cg Ser at g vb t gcc A a gt t ValI ct g Le u ac g Thr t ca Ser 140 t cc Ser gcc A a ggc G31y ggc G31y gt g ValI 220 gca A a gct A a ct g Le u gt g ValI ggt G31y 300 t gc Cys cgc Ar g gga G31y ggt G31y tt g Le u 125 gcg A a cga Ar g aaa Ly s tt t Ph e gt g ValI 205 t gg Tr p cgc Ar g gt t ValI ac c Thr ct c Le u 285 ct g Le u gca A a cgt Ar g tt g Le u t ca Ser 110 ggc G31y Le u gga G31y at t I Ie ag c Ser 1 90 ggc G31y gt g ValI t ca Ser ct c Le u c aa O31n 270 g aa O31u t gt cys g aa O31u aaa Ly s ggc O31y 95 gt c ValI c ac Hi s gca A a at t I Ie ct g Le u 1 75 t gg Tr p ag c Ser tt c Ph e cgc Ar g ct t Le u 255 c aa O31n gct A a ct t Le u cca Pr o cct Pr o 80 g at As p at c I Ie t ac Tyr gt t ValI ag c Ser 1 60 cct Pr o gag (31u at t I Ie at c I Ie agt Ser 240 tt g Le u gag (31u gt t ValI t cg Ser ct g Le u 320 c ac Hi s t at Tyr gcc A a gt a ValI ag c Ser 145 c aa O31n ct g Le u aag Ly s tt t Ph e ggt O31y 225 g at As p ct g Le u ct c Le u gt t ValI gt t ValI 305 gcg A a ct c Le u gt a ValI c aa O31n ccg Pr o 130 gct A a gct A a tt g Le u tt c Ph e g at As p 210 at c I Ie gt c ValI gt g ValI gct A a ggt O31y 290 ct t Le u tt g Le u g at As p ggt O31y gt t ValI 115 ct g Le u tt g Le u gct A a t gc cys act Thr 1 c ag O31n g aa O31u ag c Ser t cg Ser gcg A a 275 cca Pr a ggg O31y at g vb t t ct Ser tt c Ph e 1 00 ggc O31y tt t Ph e ac c Thr tt c Ph e tt c Ph e 1 gt t ValI gt g ValI ggt O31y cga Ar g at t I Ie 260 tt a Le u t gg Tr p gcc A a gca A a ggc O31y t cc Ser t ac Tyr t cc Ser t gg Tr p tt g Le u 1 at c I Ie g at As p cgc Ar g gca A a gct A a 245 t ct Ser cca Pr a ggt O31y t at Tyr at g vb t 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 gat ggc ct c at t cca agc aaa at c ggg gcc at c aac agc cgc ggt gct Page 68 seql i st cor r ect ed. t xt Asp Gy Leu lie Pro Ser Lys lie Gy Ala lie Asn Ser 330 335 Ar g G y Al a 340 gcc Al a at c SIle gct Al a gt c Val 390 aca Thr aaa Lys ttt Phe ctt Leu cag G n 470 gct Al a at g Met ttc Phe 360 aac Asn ct g Leu ttt Phe ct c Leu gct Al a 440 ggc G y ttt Phe agc Ser cag G n ct c Leu t ac Tyr aca Thr gat Asp 410 gt c Val gaa G u at c SIle cgc Ar g ggc G y 490 at c SIle gag G u gt g Val 380 gga Gy ggt Gy tta Leu cag G n t at Tyr 460 gaa G u at t SIle t cc Ser ace Thr 365 cct Pr o aaa Lys cca Pr o gt a Val ttt Phe 445 gt g Val at c SIle acc Thr 350 acc Thr t ac Tyr gga Gy gag G u gca Al a 430 gt c Val tgg Tr p ggc G y at c SIle t ac Tyr ct g Leu at c SIle at a SIle 415 acg Thr ct c Leu aca Thr gt g Val gt g Val gt c Val ttc Phe acc Thr 400 t cc Ser gt g Val ttc Phe agg Ar g gtt Val 480 cag G n at g Met 370 gcc Al a cca Pr o cga Ar g t ca Ser gcc Al a 450 t at Tyr gt c Val at t SIle 355 gt g Val ttt Phe cat Hi s gaa G u gt g Val 435 at g Met cgt Ar g ct g Leu ttc Phe caa G n t at Tyr gcc Al a aac Asn 420 tgg Tr p gcg Al a ggc G y gt c Val 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1626 ggt ttg gtc aac gga tca cta tcg ct t G y Leu Val Asn y Ser Leu Ser Leu taaacaccga aaccttcctg cta <210> 52 <211> 501 <212> PRT <213> Corynebacter i um gl utam cum <400> 52 Val Asn Thr n Ser Asp Ser Ala y 1 5 Ser Arg Thr Val Ser Ile Arg Thr Leu 25 Thr Val y Ala y Ile Phe Ser lie 40 Ala y Pro y Ala IVet Leu Ile y 55 IVet Leu Ser Val Al a Phe Val Phe Hi s 70 His Leu Asp Ser G y Val Tyr Ala Tyr Ser G n G y 10 I le Al a Leu Pro n Asn Trp Leu II e Val Leu Al a Al a Ar g Val Page 69 Al a I e I e Al a Ar g Sy Al a I e Sy Sy Ar g Leu 500 Al a Sy Ser Val Lys Sy seqi i st car r ect ed. t xt Tyr A a ValI Ser 145 O31n Le u Ly s Ph e G31y 225 As p Le u Le u ValI ValI 305 A a As n I Ie Ser Ser 385 Hi s Ar g G31y ValI 115 Le u Le u A a cys Thr 1 95 O31n O31u Ser Ser A a 275 Pr o O31y vb t Ar g I Ie 355 ValI Ph e Hi s O31u Se r Se r Ala Tr p Tyr Ser Tr p Le u 1 65 li1e As p Ar g A a A a 245 Ser Pr o O31y Tyr vb t 325 A a I Ie Le u Le u G31y 405 Ar g A a O31n Le u 150 Thr I Ie Le u G31y Ser 230 Thr Ser As p A a ValI 310 As p A a I Ie A a ValI 390 Thr Ly s Thr As p 135 ValI Thr Le u Tr p I Ie 215 ValI ValI Le u As n A a 295 Ser G31y Tr p I Ie Thr 375 vb t Ar g Hi s G31y 1 05 Ph e Pr o G31y Thr A a 1 85 Ar g ValI Ser G31y Ph e 265 vb t I Ie O31n I Ie A a 345 Ph e Le u A a As p I Ie 425 Tyr Ph e Ph e ValI Thr 1 70 Ph e As p Tyr Ar g Ph e 250 O31y A a Ser vb t Pr o 330 O31n Le u Tyr Thr As p 410 ValI Tr p Leu O31y Ser ValI ValI 1 55 ValI Le u O31y Thr O31n 235 ValI ValI Ser Le u Le u 315 Ser Le u As n Le u Ar g 395 Ser (31y Thr Ser 140 Ser A a O31y O31y ValI 220 A a A a Le u ValI O31y 300 cys Ly s I Ie O31u ValI 380 O31y O31y Le u Le u 125 A a Ar g Ly s Ph e ValI 205 Tr p Ar g ValI Thr Le u 285 Le u A a I Ie Ser Thr 365 Pr a Ly s Pr a ValI Ser 110 (31y Le u Oy le Ser 1 O31y ValI Ser Le u O31n 270 O31u cys O31u O31y Thr 350 Thr Tyr O31y O31u A a 430 Val I Ie Hi s Ty r A a Val I I e Ser 1 Leu Pro 1 Trp O31u Ser I Ie Phe I I e Ar g Ser 240 Leu Leu 255 O31n (1u A a Val Leu Ser Pro Leu 320 A a I I e 335 1I e Val Ty r Val Leu Phe I I e Thr 400 1 1 e Ser 415 Thr Val Tyr Ser Val Tr p Leu Phe Tyr Ala Ala O31u Pro O31n Phe Val Leu Phe 435 445 Page a3 y Al a 450 1 1 e Ty r 465 Val Val G3 y Ser seqI i st car r ect ed. t xt I'bt Ala I'bt Leu Pro G31y Leu I Ie Pro Tyr Val Trp Thr Ar g 455 460 Ar g (1y (1u 1n Val Phe Asn Ar g Phe (1u I Ie 1y ValI Val 470 475 480 Leu Val Val Al a Al a Se r Al a O31y Val I Ie O31y Leu Val Asn 485 490 495 Leu Ser Leu 500 <210O> 53 <21 1> 822 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (799) <223> RXA01394 <400> 53 gagcaaagt g t ccagt tgaa t ggggt tcat gaagct at at t catt t tact t aagt act tc cat aggt cac gat ggt gat c t aaaccat gt t aagaaccaa at g gaa at c t tc at t 11! I'bt Ou liePhe Ilie ac a Thr aat As n gt t ValI ac c Thr at t I Ie gca Al a g aa O31u gcg Al a g at As p ac c Thr ct g Le u ct g Le u ct c Le u ggc G31y cgc Ar g aaa Ly s ac a Thr gcc Al a 120 c ag O31n tt g Le u tt g Le u at t I Ie t gt cys g at As p ggt G31y gcc Al a cca Pr o g ac As p gt t ValI ccg Pr o ggg G31y aaa Ly s t t a Le u ct t Le u ggc G31y 75 at g vb t acc Thr ac g Thr t gg Tr p aat As n gcc Al a c aa O31n at t I Ie tt g Le u at c I Ie ac a Thr gt g ValI cgc Ar g gt a ValI 140 gcg Al a agt Ser gga G31y t ct Ser 45 t cc Ser gct Al a aac As n ccc Pr o aac As n 125 aag Ly s t at Tyr ct t tt a Leu Leu 15 at t aag I e Lys gac gt c As p Val aat gcc As n Al a t ac ct g Tyr Leu aag gt g Lys Val 95 gat gac As p As p 110 cgg gt g Ar g Val ccc at g Pr o Ib t ttg gac Leu Asp ct g Le u cgc Ar g tt t Ph e gcg Al a tt a Le u 80 g aa O31u ac g Thr cgg Ar g tt g Le u gcg Al a 1 t cc at c Ser I Ie gaa gga t tg tt c Leu Phe 50 ccg at c Pr o I Ie 65 t gg tt t Tr p Phe gcg cca Al a Pr o cct tt g Pro Leu gt g gag Val O31u 130 at g gca [ib t Al a 145 t tt gt g Phe Val gga G31y ct c Le u 35 at c I Ie gt g ValI gcc Al a c ag O31n G31y 115 gt g ValI at c I Ie tt t Ph e ccg Pr a 20 at t I Ie gcc Al a ct c Le u gt c ValI at c I Ie 1 00 ggt G31y ag c Ser gt g ValI at c I Ie c ag O31n gcg Al a ggc O31y g at As p at g vb t at t I Ie t cg Ser gt c ValI ct g Le u ggc (31y Page 71 1 50 ggc gt G3 y VE a3 y A gca gc A a A at c ae I Ie Ac, 21 at g t t I'b t L E 230 <210O> <211 <21 2> <21 3> ggC G31y Ph e gca A a 200 gt c ValI at g vb t seqI i st car r ect ed. t xt 155 160 caa tac ggc gac acc gga cgg tgg att ttc gcc O31n Tyr G31y As p Thr G31y Ar g Tr p I Ie Phe Ala 170 175 180 gca agc ct g at c t gg t tc ccg ct g gt g ggt tt c Ala Ser Leu Ilie Tr p Phe Pr o Leu Val G31y Phe 190 195 tca cgc ccg ctg tcc agc ccc aag gtg tgg cgc Ser Ar g Pr o Leu Ser Ser Pr o Lys Val Tr p Ar g 205 210 gt g gca gt t gt g at g acc gca t tg gcc at c aaa Val Ala Val Val I'b t Thr Ala Leu Ala Ilie Lys 220 225 t agtt t tcgc gggtt t tgga at c 54 233 PmT Cor ynebact er i urn gi ut anri cumn <400> 54 I'bt O31u I Ie Phe I Ie Thr G31y Leu Leu Leu G31y Ala Ser Leu Leu Leu Ser (31u Le u Pr o Tr p A a Pr o ValI vb t 145 Ph e Tr p G31y Le u li1e ValI A a O31n G31y 115 ValI I Ie Ph e Ph e O31n A a G31y As p vb t li1e Ser ValI Le u G31y 1 65 A a As n ValI Thr I Ie 70 A a (31u A a As p Thr 150 G31y G31y 10 Val I Ie Val Cys Val As p Trp G31y As p A a 90 O31u Pr o 1 Thr As p Ar g Val As n Pr o A a 03 n 1 70 A a A a 1 Ly s Le u Le u G31y vb t Thr Thr Tr p As n 1 Tyr Ser G31y Ser Ser A a As n Pr o As n 125 Ly s Tyr As p I Ie Ly s ValI A a Le u ValI As p ValI vb t As p G31y 1 Ph e Leu Val G31y 1 95 Phe Gy Ala Ala Ala Leu Ser Arg Pro Leu Ser Ser Pro 200 205 Page 72 seqi i st car r ect ed. t xt Lys Val Tr p Ar g Tr p I Ie Asn Val Val Val Ala Val Val l'bt Thr Ala 210 215 220 Leu Ala I Ie Lys Leu l'bt Leu l'b t (31y 225 230 <210O> <21 1> 1026 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1003) <223> RXA00865 <400> t tat cggaat gt ggct tggg cgat tgt tat gcaaaagt tg t taggtt t tt t gcggggt tg t t taaccccc aaat gaggga agaaggt aac ct tgaact ct at g agc aca ggt tt a l'bt Ser Thr 03 y Leu ac a Thr gt t ValI g aa G31u gcg A a g aa G31u at c I Ie g aa G31u t at Tyr at t I Ie cgg Ar g 1 50 aag Ly s cca Pr o gcg A a ac c Thr ct c Le u ggt G31y gct A a t cc Ser 120 gca A a ggt (31y gga G y ac g Thr t at Tyr ggt G3y gcc A a gga G3y t ct Ser ccg Pr o ac a Thr cca Pr o gt a ValI gaa G31u t t g Le u gaa G31u gt t ValI 75 acc Thr gct A a ag c Ser gag G31u at t I Ie 155 gag G u t cc Ser gt t ValI t cc Ser cgt Ar g aac As n ggc G3y c aa (3n gt t ValI 140 gag (3u c ac Hi s gga G31y g at As p 45 cca Pr o gag G31u aac As n gca A a gag G31u 125 cca Pr a t ct Ser tt c Ph e g ac As p aag Ly s ac g Thr g aa G31u ac g Thr g ac As p 110 gga G31y at t I Ie g at As p ggc G y 15 at c G3y ac a Thr gt t ValI cgg Ar g 95 ggc G3y tt g Le u t gt cys ac c Thr ac c Thr g at As p tt g Le u ac c Thr ggg G31y 80 ac a Thr ct t Le u ct g Le u ct c Le u at g vb t 1 60 aag Ly s 1 gt t ValI at c I Ie g at As p gcc A a 65 g at As p t ct Ser tt a Le u gcg A a t at Tyr 145 ag a Ar g ggt G31y gga G y gct A a t ct Ser 50 gct A a cgg Ar g gt g ValI gt t ValI c ac Hi s 130 g ac As p cgc Ar g g ac As p gt a ValI gct A a 35 tt g Le u g aa G31u gcg A a g aa G31u gt a ValI 115 tt c Ph e at t I Ie ct g Le u ct c Le u gca A a 20 ggc G31y gt t ValI aaa Ly s aag Ly s ct t Le u 1 00 act Thr ggt G31y cct Pr a agt Ser gt t ValI 1 at g vb t cgc Ar g ct c Le u ct a Le u ct c Le u gcg A a cct Pr a gca A a ggt G31y g aa G31u 1 gca A a t ta cct acg at t t tg gcg gt c aag gac gcc Leu Pro Thr I Ie Leu Ala Val Lys Asp Ala Page 73 seql i gcc acg tca ttg atc aaa gaa acg gga Ala Thr Ser Leu Ile Lys G u Thr y 185 190 gac cca cta aac ctt gtt tgg ctt gct Asp Pro Leu Asn Leu Val Trp Leu Ala 200 205 tcc gta att gga cat gca gcc ccc aca Ser Val IIe G y His Al a Ala Pro Thr 215 220 agc ttc gag gaa ggc gac ctc gtc cgt Ser Phe G u G u y Asp Leu Val Arg 230 235 cta tca ccg ctg gta gct gcc caa ggt Leu Ser Pro Leu Val Al a Ala G n y 250 gca aaa gct gct ctg cgt ctg cag ggc Ala Lys Ala Ala Leu Arg Leu G n y 265 270 ctt cca att atg gct cca aat gag cag Leu Pro lie IVet Ala Pro Asn Gu G n 280 285 gac atg aaa aaa gct gga gtt cta taa< Asp IVet Lys Lys Ala G y Val Leu 295 300 <210> 56 <211> 301 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 56 NIet Ser Thr G y Leu Thr Ala Lys Thr 1 5 Val y Val Ala Met Val Thr Pro Phe 25 Ile Ala Ala Gy Arg u Val Ala Ala 40 Asp Ser Leu Val Leu Al a G y Thr Thr 55 Ala Ala G u Lys Leu G u Leu Leu Lys 70 Asp Arg Ala Lys Leu Ile Ala y Val Ser Val G u Leu Ala G u Ala Ala Ala 100 105 Leu Val Val Thr Pro Tyr Tyr Ser Lys 115 120 Al a Hi s Phe G y Al a I e Al a Al a Al a 130 135 Tyr Asp lie Pro G y Arg Ser G y lie 145 150 st cor r ect ed. t xt ctt gcc tgg tat tca ggc Leu Ala Trp Tyr Ser y 195 ttg ggc gga tca ggt ttc Leu G y G y Ser G y Phe 210 gca tta cgt gag ttg tac Ala Leu Arg G u Leu Tyr 225 gcg cgg gaa atc aac gcc Ala Arg u lie Asn Ala 240 cgc ttg ggt gga gtc agc Arg Leu Gy y Val Ser 255 260 atc aac gta gga gat cct Ile Asn Val y Asp Pro 275 gaa ctt gag gct ctc cga G u Leu G u Ala Leu Arg 290 atat gaa t gat t cccga aat gat Asp att SIe aca Thr aaa Lys 245 ttg Leu cga Ar g gaa Gu 1026 G y Val 10 Thr G u Tyr Leu G y G u Al a Val 75 G y Thr 90 Ser Al a Pro Ser Thr G u Pro lie 155 Page 74 G u Hi s Phe Ser G y Asp Val Asp Lys Ser Pro Thr Arg G u G u Asn Asn Thr G y Al a Asp 110 Gn u y 125 Val Pro lle 140 G u Ser AsD G y Thr Ile Asp G y Leu Thr Thr Val G y Arg Thr G y Leu Leu Leu Cys Leu Thr Met 160 seql i st cor r ec Arg Arg Leu Ser G u Leu Pro Thr Ile Leu Ala 165 170 Sy Asp Leu Val Ala Al a Thr Ser Leu Ile Lys 180 185 Trp Tyr Ser y Asp Asp Pro Leu Asn Leu Val 195 200 G y Ser G y Phe I I e Ser Val I I e G y His Ala 210 215 Arg G u Leu Tyr Thr Ser Phe G u G u Gy Asp 225 230 235 G u Ile Asn Ala Lys Leu Ser Pro Leu Val Ala 245 250 Gy y Val Ser Leu Al a Lys Ala Ala Leu Arg 260 265 Val G y Asp Pro Arg Leu Pro Ile IVet Ala Pro 275 280 G u Al a Leu Arg G u Asp Iet Lys Lys Al a G y 290 295 <210> 57 <211> 1071 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1048) <223> RXS02021 <400> 57 ttgggtcgcc gaggagatct aatcctggtt tgagttcaga aaaccttagt taaaacatga tggaagcggt cgattaaaaa ted. txt Val Lys G u Thr Trp Leu 205 Ala Pro 220 Leu Val Al a n Leu G n Asn u 285 Val Leu 300 Asp Gy 190 Al a Thr Ar g Gy Gy 270 G n Al a 175 Leu Leu Al a Al a Ar g 255 I e G u cgc Ar g at c SIle tgg Tr p aac Asn cgc Ar g caa G n gcc Al a gac Asp gaa G u ct c Leu gt c Val cca Pr o gca Al a tgg Tr p t ac Tyr cca Pr o cag G n gac Asp gtt Val tac Tyr cca Pr o cgg Ar g gt a Val 75 gcg Al a gga Gy cca Pr o ttg Leu at g Met gca Al a cac Hi s at c SIle gaa G u gaa G u 45 ct g Leu gt c Val gat Asp gca Al a ccc Pr o gt g Val cag G n cgc Ar g gtt Val aat at c Asn Il e 15 caa att Gn lle ggc acc G y Thr ttg gta Leu Val acc gtt Thr Val 80 tac ct g Tyr Leu Page gt t cacaggt ttaagcctac atg agt gaa aac att IVet Ser Gu Asn Ile 1 gcc atg gac ggg acc Al a IVet Asp G y Thr ttc aac ccg gat cag Phe Asn Pro Asp G n aca cgc ctc gga gca Thr Arg Leu y Ala aaa ctg gac caa gat Lys Leu Asp G n Asp atc ccc gat ctg tct lie Pro Asp Leu Ser cgc ctc cac ctg ctt Arg Leu His Leu Leu seql i st cor r ect ed. t xt 95 100 tcc cac cgg ctg gtc cgc ccc cac gaa atg cac atg caa aac acc ttg 451 Ser His Arg Leu Val Arg Pro His G u Iet His IVet n Asn Thr Leu 105 110 115 gag ctg ctg tcc gac gtg gtg tgg aca aac aag ggc cct tgc ctt cct 499 G u Leu Leu Ser Asp Val Val Trp Thr Asn Lys y Pro Cys Leu Pro 120 125 130 gaa aac ttt gag tgg gtg cgt ggt gct ctg cgg tcc cgc gga ctc atc 547 G u Asn Phe G u Trp Val Arg y Ala Leu Arg Ser Arg y Leu Ile 135 140 145 cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595 His Val Tyr Cys Val Asp Arg Leu Pro Arg IVet Val Asp Tyr Val Val 150 155 160 165 ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643 Pro Pro y Val Arg Ile Ser G u Ala G u Arg Val Arg Leu y Ala 170 175 180 tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691 Tyr Leu Ala Pro y Thr Ser Val Leu Arg G u y Phe Val Ser Phe 185 190 195 aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739 Asn Ser y Thr Leu G y Ala Ala Lys Val G u y Arg Leu Ser Ser 200 205 210 ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787 Sy Val Val Ile Gy u y Ser u lie y Leu Ser Ser Thr Ile 215 220 225 cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835 G n Ser Pro Arg Asp G u G n Arg Arg Arg Leu Pro Leu Ser Ile y 230 235 240 245 caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883 G n Asn Cys Asn Phe G y Val Ser Ser Gy lie lie y Val Ser Leu 250 255 260 gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931 Sy Asp Asn Cys Asp Ile y Asn Asn lie Val Leu Asp y Asp Thr 265 270 275 ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979 Pro lie Trp Phe Ala Al a Asp G u G u Leu Arg Thr Ile Asp Ser Ile 280 285 290 gaa ggc caa gca aat tgg tca atc aag cgt gaa tcc ggc ttc cat gag 1027 G u G y G n Ala Asn Trp Ser IIe Lys Arg u Ser G y Phe His G u 295 300 305 cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgc 1071 Pro Val Ala Arg Leu Lys Ala 310 315 <210> 58 <211> 316 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 58 IVet Ser Ou Asn Ile Arg Oy Ala On Ala Val Oy Ile Ala Asn lie 1 5 10 Page 76 Ala IVbt Asp seql i st cor r ect ed. t xt Thr Ile Leu Asp Thr Trp Tyr Pro u Pro n Ile Phe Thr Lys I le Ar g bet Gy Ser 145 Val Val Gy Gy Leu 225 Pr o SIle Leu Thr Ser 305 Pr o Leu Asp Asp Hi s Asn 115 Cys Gy Tyr Leu Val 195 Leu Ser Ser Val Gy 275 Asp Phe G n Al a Asp Ser Leu Leu Pr o SIle Val 165 Al a Phe Ser SIle Gy 245 Leu Thr SIle Al a G u Leu Pr o Hi s Leu Asn 135 Val Pr o Leu Ser Val 215 Ser Asn Asp SIle Gy 295 G u 40 Leu Val Pr o Ar g Leu 120 Phe Tyr Gy Al a Gy 200 Val Pr o Cys Asn Tr p 280 G n Ar g Thr G u Val Leu 105 Ser G u Cys Val Pr o 185 Thr SIle Ar g Asn Cys 265 Phe Al a Tyr Pr o G n Asp 90 Val Asp Tr p Val Ar g 170 Gy Leu Gy Asp Phe 250 Asp Al a Asn Pr o Ar g Val Al a Ar g Val Val Asp 155 I le Thr Gy G u G u 235 Gy SIle Al a Tr p G u Leu Val Asp Hi s Tr p 125 Gy Leu G u Val Al a 205 Ser Ar g Ser Asn G u 285 I le Val G n Ar g Val G u 110 Thr Al a Pr o Al a Leu 190 Lys G u Ar g Ser Asn 270 G u Lys Gy Leu Thr Tyr bet Asn Leu Ar g G u 175 Ar g Val SIe Ar g Gy 255 I e Leu Ar g His u Pro Val Ala Arg Leu Lys Al a 315 <210> 59 <211> 1296 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1273) <223> RXS02157 Page 77 seql i st cor r ect ed. t xt <400> 59 gggtggaatt ggcacgatgg tgctgccgga tgtttttgat caccgttttt agaaaagacg acaaggatgg ggaactgtaa cgggagaatt atcctgaagg atg agc acg ctg gaa 11! lMt Ser Thr Leu G u act Thr ct g Leu t ac Tyr cac Hi s cac Hi s gag G u acc Thr ttc Phe cat Hi s cca Pr o 150 t ac Tyr cca Pr o ggc Gy tgc Cys gtt Val 230 cca Pr o t cc Ser gac Asp gcg Al a t ca Ser at c SIle gtt Val at t SIle 120 ttc Phe aag Lys t ac Tyr gat Asp gtt Val 200 gag G u cgt Ar g gt c Val aag Lys ct c Leu at c l Ie ttg Leu cgt Ar g ttc Phe cgc Ar g ggc G y gaa G u gac Asp 170 gct Al a gca Al a ggc gcy ggc Gy at t SIle ggc Gy gcg Al a gag G u tt c Phe 75 ttt Phe tgc Cys tt g Leu cgc Ar g gcg Al a 155 acc Thr gct Al a cct Pr o at c SIle gat Asp 235 at t SIle gca Al a ggc Gy gcg Al a gca Al a tcg Ser aac Asn act Thr acc Thr 140 ttc Phe gat Asp at c l Ie gaa G u ttg Leu 220 ttc Phe acg Thr gt c Val gca Al a acc Thr agg Ar g gac Asp ggc G y 110 cgt Ar g ggt G y cca Pr o ttg Leu ct c Leu 190 ttc Phe at c SIle gca Al a ggc Gy gat Asp aac Asn cag G n gt c Val gcc Al a gaa G u cgg Ar g ct c Leu cca Pr o 160 aaa Lys cca Pr o aag Lys gat Asp cag G n 240 1 acc Thr gac Asp gcg Al a at c SIle gt c Val acc Thr gca Al a at t SIle gcg Al a 145 agc Ser at g Met at c SIle gca Al a gaa G u 225 cac Hi s cca Pr o cag G n ttg Leu ggc Gy gag G u ct c Leu aac Asn ct g Leu 130 ct g Leu ggt G y gt a Val cag G n gt g Val 210 gt c Val gat Asp cca Pr o ggc G y ggc G y caa G n gt c Val gcc Al a gag G u 115 gct Al a act Thr gt g Val gaa G u ggt G y 195 cgc Ar g cag G n ggc Gy gtt Val aat Asn cac Hi s ctt Leu gcc Al a gcg Al a 100 gct Al a gca Al a ggc G y gag G u acc Thr 180 gaa G u gag G u act Thr gtt Val ccc gat gtg gtg acc atg gcc aag gga ctt ggc ggc ggt ctt ccc atc Page 78 seqi i st car r ect ed. t xt Pro Asp Val Val Thr l'bt Al a Lys G31y Leu G31y (31y (31y 250 255 Leu Pro I Ie 260 ggt G31y aag Ly s aag Ly s cgc Ar g 310 gt a ValI g ac As p t tg Le u at c I Ie t gt cys ggc G31y 280 gt g ValI G31y gt c ValI gca Al a gca Al a 360 g ac As p gcc Al a act Thr t ct Ser ct g Le u ggc G31y 330 c aa O31n gcg Al a g aa (31u act Thr t t c Ph e gt t ValI t t c Ph e 315 ag g Ar g gct Al a gac As p at c I e ggc G31y ggt G31y gt c ValI 300 aag Ly s ggc G31y gt t ValI aac As n gca Al a 380 gca Al a 270 aac As n g ac As p ct t Le u at g vb t g at As p 350 at c I Ie gca Al a gct Al a cca Pr o gct Al a ct t Le u tt g Le u 335 ggt G31y cgt Ar g g aa (31u gt t ValI tt c Ph e gcc Al a 320 ggc G31y tt t Ph e tt g Le u tt g Le u gct Al a t gc cys 305 aag Ly s gt g ValI aag Ly s ac c Thr at g vb t t gt cys 290 gca Al a gt t ValI gt g ValI c ac Hi s ccg Pr o 370 ac c Thr 275 gca Al a g aa (31u g ac As p ct g Le u ggc G31y 355 ccg Pr o cca Pr o gct Al a gt t ValI G31y gag (31u 340 gt t ValI ct g Le u ggc G31y gcc Al a gcc Al a gt t ValI 325 cgc Ar g at t I Ie gt g ValI 931 979 1 027 1 075 1123 1171 1219 1267 1296 gt c aag gct at Val Lys Ala 11 385 t gcc gag aca e Al a 03 u Th r at c gca t aaaggact c aaact tat ga ct t I e Al a 390 <210O> <21 1> 391 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> l'bt Ser Thr Leu O31u Thr Tr p Pro O31n 1 5 Thr Pr o Pr o Val O31u Leu Val Ser G31y 25 Asp O31n G31y As n Val Tyr I Ie As p Leu 40 Al a Leu G31y Hi s Al a Hi s Pr o Al a I Ie 55 lie G31y O31n Leu G31y Hi s Val Ser As n 70 Val O31u Val Al a O31u O31u Leu I Ie Lys Thr Leu Al a Ala O31n Th r Ar g Val Phe 100 105 Al a As n 03 u Al a Al a Ph e Lys I I e Al a Val I Ie I Ie 10 Lys 03 y Al a Leu Al a (3 y I e 03 u Al a Leu Phe Al a Ar g Phe Ser 90 Phe Cys Asn Arg Leu Thr Page 79 As n Thr I Ie ValI Ser Le u Ser (31y Thr ValI Al a Thr Ar g As p (31y 110 Ar g Tyr Thr ValI As n Pr a As p Al a Se r seql i st cor r ect ed. t xt 120 Phe His y Arg Thr 125 ebt y Ser Leu SIle Al a 145 Ser bet SIle Al a G u 225 Hi s Gy Leu Al a Cys 305 Lys Val Lys Thr Al a 385 Al a Thr Val G u Gy 195 Ar g G n Gy Leu Thr 275 Al a G u Asp Leu Gy 355 Pr o Al a Val G n Phe 165 Asn Thr Leu Gy Val 245 I le Gy Al a Al a Val 325 Ar g SIle Val Thr Hi s G y 135 Pro Asp 150 Tyr Pr o Pro Thr G y Val Cys Asp 215 Val G y 230 Pro Asp G y Al a Lys Hi s Lys Al a 295 Arg Lys 310 Val Asp Asp Val Leu Asn Ile Thr 375 I e Al a 390 Lys Tyr Asp Val 200 G u Ar g Val Cys Gy 280 Val Gy Val Al a Al a 360 Ar g Gy Val 185 Pr o Tyr Thr Val Leu 265 Thr Leu G u Ar g Lys 345 Pr o u Asp 170 Al a Al a Gy Gy Thr 250 Al a Thr Ser Leu Gy 330 G n Al a Al a 155 Thr Al a Pr o SIle Asp 235 bet Thr Phe Val Phe 315 Ar g Al a Asp 140 Phe Asp l Ie G u Leu 220 Phe Al a Gy Gy Val 300 Lys Gy Val Asn Al a 380 Leu Tyr Phe Gy 205 Met Phe Lys Ar g Gy 285 Asp G u Leu Leu SIle 365 Pr o Leu Leu 190 Phe SI e Al a Gy Al a 270 Asn Asp Leu Met Asp 350 I le NMbt Ar g 175 G u Leu Thr Hi s Leu 255 Al a Pr o Al a Leu Leu 335 Gy Ar g Pr o 160 Lys Pr o Lys Asp G n 240 Gy G u Val Phe Al a 320 Gy Phe Leu Asp u u Ile Asp Al a Val Lys <210> 61 <211> 1008 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(985) <223> RXC00733 <400> 61 acggcgaggt tgtcggtatt ggaacgcaca cgaatttgct gaacacgtgc ggtacctacc gtgaaattgt tgaatcccaa gagactgcgc aggcgcaatc atg agt aat act gca 115 Page seql i st cor r ect ed. t xt bet Ser Asn Thr Al a ggc G y gca Al a cat Hi s gtt Val gt g Val aag Lys gct Al a ttt Phe at c SIle gt g Val 150 cac Hi s ctt Leu caa G n ttg Leu gt a Val 230 t cc Ser cgc Ar g aat Asn cgt Ar g ctt Leu ttt Phe gat Asp at g lbt aaa Lys 120 agc Ser agt Ser ct a Leu cgt Ar g ttg Leu 200 at g lbt at t SIle aaa Lys cgt Ar g gga G y acc Thr gt c Val gga G y at c SIle gaa G u gcc Al a ttg Leu at g let ct g Leu 170 acc Thr cag G n ttt Phe gt c Val ttt Phe 250 t cc Ser cca Pr o tta Leu tt g Leu tt c Phe 75 gcg Al a gac Asp at g Mbt tcg Ser cac Hi s 155 agc Ser aac Asn gcg Al a at c SIle acc Thr 235 gcg Al a cat Hi s tct Ser at t SIle ggc G y ct a Leu cag G n at g lbt at c SIle ttg Leu 140 cgg Ar g t at Tyr gat Asp at c l Ie at c SIle 220 at c SIle gaa G u cag G n gcc Al a ttt Phe 45 cca Pr o tct Ser ttg Leu aac Asn ct c Leu 125 ttc Phe ct g Leu ttc Phe gt g Val act Thr 205 t cc Ser gt g Val cag G n gca Al a aaa Lys gtt Val tgg Tr p aag Lys cag G n ctt Leu 110 gga G y cag G n cgc Ar g gat Asp gat Asp 190 t cc Ser cca Pr o gt c Val tgg Tr p gac Asp 15 agg Ar g at c SIle ttg Leu cgc Ar g gct Al a 95 gtt Val ct g Leu gcg Al a at g let t cc Ser 175 aat Asn ct a Leu ct g Leu act Thr aag Lys 255 gcc Al a ctt Leu ttc Phe ct g Leu at g lbt 80 gca Al a cca Pr o gt g Val cgg Ar g gag G u 160 at c SIle at c SIle ct g Leu ct c Leu gt g Val 240 cag G n gcg Al a ttc Phe ct a Leu ggt G y ccg Pr o ggt G y ggc G y at c SIle at g lbt 145 gt g Val aaa Lys ggt G y acc Thr gca Al a 225 gt g Val acc Thr ccg Pr o gga G y gcc Al a aaa Lys gct Al a aaa Lys t ca Ser ggt G y 130 ct c Leu gag G u cgt Ar g caa G n gt c Val 210 ct c Leu gtt Val ggt G y aat Asn at t SIle gt c Val gcc Al a ggt G y cat Hi s ggc G y 115 gct Al a aac Asn gaa G u ggt G y t cc Ser 195 at c SIle gt g Val gcg Al a at t SIle caa G n ct a Leu ct g Leu acc Thr gcg Al a aat Asn 100 at t SIle t at Tyr cgc Ar g aaa Lys gat Asp 180 ct g Leu ggt G y gcg Al a agc Ser ttg Leu 260 aag Lys ggc G y agc Ser aac Asn t ca Ser cag G n gat Asp ct c Leu at c SIe at c SIe 165 ctg Leu caa G n gtg Val ctg Leu cgt Ar g 245 aat Asn gcg cgc ctg gag gaa acc tac tct ggc cac gcc gtg gtt aag gtt ttc Page 81 seqI i st car r ect ed. t xt Ala Arg Leu Ou Ou Thr Tyr Ser Gy His Ala Val Val Lys Val Phe 265 270 275 gga cac caa aag gat gtt caa gaa gca ttc gag gaa gaa aat caa gct O31y Hi s (1n Lys As pVa1(3 On 1u Ala Ph e (1u (1u 1u As n 1n Aa 280 285 290 t gt gt a t aaggccagc t t t ggt gccc agt Cys Val 295 <210> 62 <211> 295 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 979 1 008 <400> 62 I'bt Ser Asn Thr Ala O31y Pr o Ar g (31y A a Ph e Le u O31y Pr a O31y O31y I Ie vb t 145 ValI Ly s O31y Thr A a 225 ValI As n I Ie ValI A a O31y Hi s O31y 115 A a As n O31u O31y Ser 1 95 li1e ValI A a Ly s O31y Ser As n Ser O31n As p Le u I Ie I Ie 1 65 Le u O31n ValI Le u Ar g 245 A a Hi s ValI ValI 70 Lys5 A a Ph e I Ie ValI 150 Hi s Le u O31n Le u ValI 230 Ser O31n As p O31y ValI O31u Ser O31u O31y 135 O31n Ar g Ser Thr ValI 215 Ser O31n Ph e As n Thr O31u I Ie vb t 1 05 Le u Le u A a Pr a ValI 1 85 Ser vb t Pr a Le u Ser Pr a Le u Le u Ph e A a As p vb t Ser Hi s 1 Ser As n A a I Ie Thr 235 A a O31n A a Ph e Pr a Ser Le u As n Le u 125 Ph e Le u Ph e ValI Thr 205 Ser ValI O31n A a Lys5 ValI Tr p Lys5 O31n Le u 110 O31y O31n Ar g As p As p 1 Ser Pr a ValI Tr p As p Ar g I Ie Le u Ar g A a ValI Le u A a vb t Ser 1 As n Le u Le u Thr 255 Thr cOy Ilie Leu Asn Ala Arg Leu cOu Ou Thr Tyr Ser 260 265 Page 82 03 y Hi s A a 270 seqI i st car r ect ed. t xt Val Val Lys Val Phe (31y His O31n Lys Asp Val (31n O31u Ala Phe O31u 275 280 285 G3 u G3 u As n O n Al aCys Val 290 295 <210> 63 <211> 426 <212> DNA <213> Cor ynebact er i urn gi ut anm curn <220> <221> CIDS <222> (426) <223> RXCO0861 <400> 63 at g cct Pr o act Th r ggt G31y ggt G31y c ac Hi s aag aag gca A a cct Pr o gct A a cgt Ar g g aa O31u act Th r t ac Ty r gct A a ct g Le u 115 aac As n aag Lys5 ct g Le u gga G31y gca A a gt t ValI gga G31y cct Pr o at c I Ie gt t ValI at g vb t cgc Ar g tt g Le u tt c Ph e ggt G31y ct g Le u c ac Hi s act Th r gt t ValI 135 at t I Ie gcg A a at c I Ie gt c ValI g ac As p tt c Ph e gag O31u 1 05 gt t ValI at g vb t ac c Th r 10 cgt Ar g ct t Le u at c I Ie gcc A a tt g Le u 90 t gg Tr p aac As n gt c ValI act Th r cgt Ar g t ct Ser aac As n aag Lys5 t ac Ty r cgc Ar g cgc Ar g aac As n act Th r c ac Hi s t cc Ser ct g Le u c ac Hi s gcc A a ct g Le u aac As n 125 cgc Ar g c ag O31n cga Ar g ct g Le u gct A a ac c Th r gct A a cgc Ar g 110 gt t ValI gca A a ggt G31y c ag O31n gt t ValI c ag O31n t cg Ser cgt Ar g gcc A a gt g ValI <210O> 64 <21 1> 142 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm curn <400> 64 I'b t Ala Pro His Lys Val I'bt Leu IlieThr Thr Gy Thr On OyOu 1 5 10 Pro I'bt A a A a Leu Se r Ar g I'b t A a Ar g Arg 0 u His Arg O1n Ile 25 Page 83 seqI i st car r ec Thr Val Arg Asp Oy Asp Leu Ilie Ilie Leu Ser 40 31y As n(1u 1u Aa ValI PheOy Val lleAsn 55 O31y Ala Thr Val Val Thr Oy Arg Asp Ala Lys 70 Hi s G3 y Ty r Ser G3 y G3 u Leu Leu Phe Leu Tyr 90 Lys Asn Ala I'b t Pr o Val Hi s O31y O31u Tr pAr g 100 105 Lys O31u Leu Ala Ilie Ser Thr O31y Val As n Ar g 115 120 Ala O31n Asn O31y Val Val Val Asp Nib t ValI As n 130 135 <210> <211> 1066 <212> DNA <213> Cor ynebact er i urn gI ut ari cumn <220> <221> CIDS <222> (1066) <223> RXCO0866 <400> gcat caacgt aggagat cct cgact tccaa t tat ggct cc ct ct ccgaga agacat gaaa aaagct ggag t t ct at aaat ted. t xt Ser Ser I'b t Leu Val Hi s As n A a Hi s Leu As p As n 125 O31y Ar g 140 Le u A a Thr A a Ar g 110 ValI A a ValI O31n Ser Ar g A a ValI aaatgagcag gaacttgagg aat As n c ag O31n t ct Ser g at As p aac As n cgt Ar g aac As n ggC O31y aac As n c ag O31n gcg A a c aa O31n aac As n aat As n aag Lys5 ct g Le u gct A a ggt O31y t cc Ser aac As n ggC O31y gt t ValI g at As p gt a ValI gct A a cag O31n 75 cgt Ar g gcg A a ac c Thr ac c Thr aaa Lys5 c aa O31n aac As n cgc Ar g aac As n cgc Ar g cct Pr o gct A a 45 gga O31y cgc Ar g ggt O31y aac As n aag Lys5 gt c ValI gag O31u t cc Ser gag O31u ggt O31y aac As n 110 gcg A a 15 tt t Ph e ac c Thr c aa O31n t cc Ser cgt Ar g 95 ag c Ser ggC O31y c ag O31n gcc A a g at As p gga O31y 80 gga O31y ggt O31y at g vb t 1 cca Pr o gca A a gga O31y t ct Ser aac As n cgt Ar g aac As n cca Pr o cca Pr o aac As n c ag O31n aac As n cgt Ar g c ag O31n g aa O31u g at As p g ac As p ggt O31y aac As n gga O31y aac As n 115 gct A a gct A a aat As n t cc Ser cgc Ar g t cc Ser 1 00 cgt Ar g aat gat t cc Asn Asp Ser cga Ar g ggt O31y t cc Ser Cgg Ar g c ag O31n aac As n gga O31y c ag O31n ggc gga aac cgt ggc aac cgc ggt ggc gga cgc cga aac gtt gtt aag Page 84 s G y G y Asn Arg G y Asn Arg G y 120 125 eql i st cor r ect ed. t xt Gy Gy Arg Arg Asn 130 Val Val Lys tcg atg cag ggt gcg gat Ser IVet Gn Gy Ala Asp 135 gca ccg gca aac ggt ctg Ala Pro Ala Asn Gy Leu 150 155 atc ggt cgc aac atg acc lie y Arg Asn IVet Thr 170 gtg gac tgt ggt gtg ct c Val Asp Cys G y Val Leu 185 ctg att ctt cct gac ttc Leu lie Leu Pro Asp Phe 200 gat gca ttg gtg gtt act Asp Ala Leu Val Val Thr 215 ccc tgg ctg ctg aag ct g Pro Trp Leu Leu Lys Leu 230 235 ttc acc ttg gct ctg att Phe Thr Leu Ala Leu Ile 250 ccg aag ctg atc gag gtc Pro Lys Leu lie G u Val 265 ttc aac att cgc ttc tgg Phe Asn lie Arg Phe Trp 280 ggt ctt gct atc aag act G y Leu Al a lle Lys Thr 295 atc aag ctg gat cag act Ile Lys Leu Asp G n Thr 310 315 <210> 66 <211> 322 <212> PRT <213> Corynebacter i um gl <400> 66 IVet Asn Asp Ser Arg Asn 1 5 Pro Pro Ou Ala Gy n Ala Pro Asp Ala Ser Ser Sy Asn Asp Asn Arg Asp ct g Leu 140 cgt Ar g gt g Val ttc Phe ggc G y cac Hi s 220 cgc Ar g gca Al a aac Asn gct Al a cct Pr o 300 cct Pr o acc Thr at t SIle ttt Phe cca Pr o cca Pr o 205 gga G y aac Asn gct Al a gag G u gtt Val 285 gct Al a cag G n t ac Tyr gag G u tct Ser 190 at t SIle cac Hi s gat Asp aag Lys cag G n 270 aac Asn ggt G y cgc Ar g gca Al a t ac Tyr 175 t ca Ser gag G u gaa G u at c SIle tgt Cys 255 t cc Ser cac Hi s ttg Leu ct g Leu ctt Leu 160 aac Asn ggt G y gat Asp gac Asp cca Pr o 240 aag Lys aat Asn t cc Ser gt c Val cca Pr o 145 ggt G y aac Asn gag G u cac Hi s cac Hi s 225 at c SIle gaa G u gag G u at c SIle at c lie 305 gag G u ggc G y cgt Ar g cca Pr o ct g Leu 210 at t SIle ttg Leu cac Hi s gac Asp cca Pr o 290 cac Hi s cca Pr o at t SIle ct g Leu ggc G y 195 cac Hi s ggt G y gca Al a cgt Ar g cgc Ar g 275 gac Asp acc Thr cca Pr o t cc Ser ct c Leu 180 gtt Val cgc Ar g gct Al a t cc Ser cag G n 260 gga G y tgc Cys ggt G y 547 595 643 691 739 787 835 883 931 979 1027 1066 cct gat gga Pro Asp G y cgc cca act Arg Pro Thr 320 ut ari cum Arg Oy Arg Lys Val 10 G u Asn His Leu Asp 25 Asn G n Ser Ala Val 40 Al a Al a n G y Al a Page Thr Arg Lys Ala y Thr Pro Val Phe G n Lys Ala G u Thr Ala Sn G y Ser G n Asp seqi i st car r ect ed. t xt Ser As n Ar g As n Ar g Pr o 145 O31y As n O31u Hi s Hi s 225 li1e O31u Ou le le 305 Pr o G31y As n G31y As n 115 ValI Pr o I Ie Le u G31y 1 95 Hi s G31y A a Ar g Ar g 275 As p Thr O31n As n G31y O31n Ly s Ly s O31u 1 65 li1e As p ValI I Ie Ar g 245 Ar g Pr o Le u As p A a O31n 03 y Se r O31n As n Ar g 03 u Se r Ser A a G31y vb t 135 Pr a O31y As p I Ie A a 215 Tr p Th r Ly s As n Le u 295 Ly s As n As n As n 120 O31n A a Ar g cys Le u 200 Le u Le u Le u Le u I Ie 280 A a Le u As n O31u 1 Ar g O31y As n As n O31y 1 Pr a ValI Le u A a I Ie 265 Ar g I Ie As p As n 90 O31y O31y A a O31y 1 70 ValI As p ValI Ly s Le u 250 O31u Ph e Ly s O31n Ar g A a As n As p Le u 1 Th r Le u Ph e Th r Le u 235 li1e ValI Tr p Th r Th r 315 Ar g As n Ar g Le u 140 Ar g ValI Ph e O31y Hi s 220 Ar g A a As n A a Pr a 300 Pr a (31y As n O31y 125 Th r I Ie Ph e Pr a Pr a 205 O31y As n A a O31u ValI 285 A a Pr a Ar g Ser O31y Ar g A a Ty r 1 Ser O31u O31u I Ie cys 255 Ser Hi s Le u (31y <210O> 67 <21 1> 1527 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1504) <223> RXC02095 <400> 67 ct ct ct t ggt cct ct cccca cccat t t t t a agt act caag accct t ccaa cagaaaggat act ccccca acaggct caa aaat act gaa aggct cacgc at g aaa act gag caa 115 Page 86 seql i st cor r ect ed. t xt IVet Lys 1 Thr G u G n tcc caa aaa gca caa tta gcc cct Ser n Lys Ala G n Leu Ala Pro cgc Ar g t ca Ser ctt Leu gga G y tta Leu ctt Leu agc Ser cag G n gt c Val 150 at g Met gt g Val ttg Leu ttt Phe cat Hi s 230 cag G n cgc Ar g acc Thr ctt Leu act Thr gt g Val t at Tyr ggg G y 120 ct c Leu cgg Ar g ccg Pr o at g Met cct Pr o 200 t cc Ser gaa G u gac Asp ctt Leu at c SIle acc Thr acc Thr acc Thr agt Ser cag G n ggg G y at t SIle ttg Leu 170 gct Al a ct g Leu tgg Tr p act Thr gag G0u 250 at t SIle agc Ser ggt G y act Thr 75 agc Ser caa G n cat Hi s cca Pr o tcg Ser 155 at c SIle at t l Ie tt g Leu tcg Ser gt c Val 235 acc Thr t cc Ser gct Al a ggc G y gac Asp gt c Val ttt Phe gat Asp ggt G y 140 gat Asp gga G y t cc Ser tgg Tr p gcc Al a 220 acg Thr gac Asp gt g Val tta Leu 45 gcc Al a ct g Leu at t SIle gga G y gt c Val 125 cag G n at c SIle aat Asn ccg Pr o gcc Al a 205 cag G n ggt G y aaa Lys aag Lys gcg Al a gct Al a at c l Ie ct g Leu gcc Al a cgg Ar g 110 cgg Ar g gac Asp aac Asn gt g Val ccg Pr o 190 gt g Val caa G n at c SIle ttg Leu aaa Lys 15 tgg Tr p gca Al a gat Asp gac Asp ctt Leu 95 cga Ar g ctt Leu tct Ser at g let gt c Val 175 ct g Leu gcc Al a aag Lys cgc Ar g gat Asp 255 gca Al a cag G n acg Thr at c SIle cgg Ar g 80 at c SIle t ac Tyr aaa Lys at t SIle gt g Val 160 aag Lys acc Thr t at Tyr gct Al a gt g Val 240 ct c Leu gaa G u cct Pr o ttt Phe ct c Leu act Thr ctt Leu gca Al a at g Met 130 aca Thr agc Ser gt g Val at c SIle cga Ar g 210 gat Asp aag Lys gca Al a aag Lys tgg Tr p gaa G u gga G y ccg Pr o ct c Leu ggc G y 115 cgc Ar g ggc G y ctt Leu ct c Leu gct Al a 195 aaa Lys ct g Leu gca Al a cgt Ar g cca Pr o ct c Leu ctt Leu aat Asn agt Ser gcg Al a 100 aag Lys t ca Ser caa G n gt g Val act Thr 180 gca Al a gcg Al a acc Thr ttt Phe gag G0u 260 caa G n acc Thr aca Thr acc Thr gga G y ttg Leu ct c Leu ttg Leu gta Val gcg Al a 165 ttg Leu gtg Val ctt Leu act Thr gcg Al a 245 tta Leu ttt gcc cag cgc atg cgc act gca cgt ctg acg gca aag ttc atc ccc Page 87 seqIi Ph e Ala O31n Ar g 265 I'bt Ar g Thr A st car r ect ed. t xt Leu Thr A a Lys a at g vb t ggt G31y gcg A a 310 t cg Ser at c I Ie ccc Pr o g at As p g aa O31u 390 gt g ValI tt t Ph e g at As p t cc Ser gag O31u 280 t at Tyr t ct Ser at g vb t g aa O31u t ca Ser 360 cgt Ar g gt t ValI ct t Le u ag c Ser cgc Ar g 440 t cc Ser ct t Le u gcc A a t at Tyr at g vb t 330 at t I Ie ccc Pr o at t I Ie t tg Le u gga G31y 410 ggc G31y aat As n ccg Pr o ccg Pr o at g vb t ct c Le u 315 cgc Ar g g at As p gac As p ct c Le u gt g ValI 395 aac As n cat Hi s ct c Le u cga Ar g c ag O31n act Thr 300 act Thr gt g ValI ct t Le u act Thr aac As n 380 ggc O31y tt t Ph e cgc Ar g at c I Ie g aa O31u 460 ct t Le u 285 ggt G31y ag c Ser c ag O31n c ag O31n ccc Pr a 365 ggt O31y cct Pr a t at Tyr act Thr gcg A a 445 cat Hi s Ar g 270 gct A a c ac Hi s tt g Le u tt g Le u cct Pr a 350 ct g Le u tt t Ph e cca Pr a c aa O31n cgc Ar g 430 gt t ValI ct c Le u tt g Le u at c I Ie t cg Ser gcg A a 335 g aa O31u ggt O31y gag O31u ggt O31y cca Pr a 415 tt c Ph e tt t Ph e g at As p gt g ValI ac g Thr gcg A a 320 ct g Le u cgc Ar g ct g Le u ct g Le u t ca Ser 400 g ac As p g ac As p g at As p ggg O31y aac As n 290 ggc O31y gct A a t ct Ser g at As p tt c Ph e 370 gt t ValI aag Ly s ggc O31y ct c Le u ccg Pr a 450 gga O31y Phe I Ie Pro 275 gt t ValI tt t Ph e t cc Ser gag O31u 340 gca A a aac As n gcc A a at g vb t at c I Ie 420 c ac Hi s tt g Le u c ag O31n ggc O31y gt g ValI ct g Le u 325 cgc Ar g c ac Hi s gt a ValI ggt O31y gct A a 405 gcc A a ag c Ser t ac Tyr 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 504 1 527 t gat gagcag at cgaacacg cag <210> 68 <211> 468 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 68 I'bt Lys Thr O31u O31n Ser O31n Lys Ala O31n Leu Ala Pro Lys Lys Ala 1 5 10 Pr o Ou Lys Pro On Ar g I Ie Ar g O31n Leu Ilie Ser Val Ala Trp On 25 Ar g Pr o Tr p Leu Thr Ser Phe Thr Val I Ie Ser Ala Leu Ala Ala Thr 40 Page 88 seqI i st car r ect ed. t xt Leu Phe O31u Leu Thr Leu Pro Leu Leu Thr O31y 03 Al a iieAsp Il e Al a Ph e ValI Th r Th r Ar g 145 O31n Le u I Ie Ser Al a 225 ValI Th r Al a ValI ValI 305 ValI Ser Th r Ser O31y 385 O31y Pr o Le u O31y 115 Ar g O31y Le u Le u Al a 1 95 Ly s Le u Al a Ar g Ph e 275 li1e Th r Ar g ValI Pr o 355 As n O31n Th r O31y Le u Le u Le u ValI Al a 1 65 Le u ValI Le u Th r Al a 245 Le u Pr o O31y ValI Le u 325 Ar g Hi s ValI (31y As p Ser Ar g vb t As n 135 Ser Le u I Ie ValI Al a 215 ValI O31u Al a ValI O31y 295 Ph e O31y Ph e Le u Ph e 375 Th r Le u Le u Al a 1 05 ValI As p Ser vb t Le u 1 85 Le u Th r O31u Ar g Ar g 265 O31n Le u Ser Le u ValI 345 Le u O31y ValI Th r Th r 90 Ser O31n O31y I Ie Le u 1 70 Al a Le u Tr p Th r O31u 250 vb t Le u Al a Ty r vb t 330 li1e Pr o I Ie Le u Th r Ser O31n Hi s Pr a Ser 1 le le Le u Ser ValI 235 Th r Ar g Pr a vb t Le u 315 Ar g As p As p Le u ValI 395 Le u I Ie O31y ValI 125 O31n I Ie As n Pr a Al a 205 O31n O31y Ly s Al a Le u 285 O31y Ser O31n O31n Pr a 365 O31y Pr a Le u Al a Ar g 110 Ar g As p As n ValI Pr a 1 ValI O31n I Ie Le u Ar g 270 Al a Hi s Le u Le u Pr a 350 Le u Ph e Pr a As p Le u Ar g Le u Ser vb t ValI 1 Le u Al a Ly s Ar g As p 255 Le u Le u I Ie Ser Al a 335 O31u O31y O31u O31y O31y Lys Thr I'bt Al a Val O31n Leu Al a O31y Asn Phe Tyr O31n Pro Asp Page 89 seql i st cor r ect ed. t xt 405 410 415 Ser Gy His lie Ala Phe Asp Ser Asn Gy His Arg Thr Arg Phe Asp 420 425 430 Asp Leu Thr His Ser Asp Ile Arg Arg Asn Leu Ile Ala Val Phe Asp 435 440 445 G u Pro Phe Leu Tyr Ser Ser Ser lie Pro Arg G u His Leu Asp G y 450 455 460 Phe G y Cys G n 465 <210> 69 <211> 295 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (84)..(272) <223> RXC03185 <220> <221> m sc feature <222> 57 <223> n a, c, g, or t <400> 69 agcgcccaac cgttcagacc agcggtttct ctgaggatgc aaagtccatg atgggtnagg t cactgagct gtccgaaacc acc at g aat gat ctt gca gct gaa ggt gaa aac IVet Asn Asp Leu Ala Ala Gu Gy Gu Asn 1 5 gat cct tac cgc atg gtt cag cag ctg cgc cgc aag ctc tct cgc ttc Asp Pro Tyr Arg IVet Val n G n Leu Arg Arg Lys Leu Ser Arg Phe 20 gtc gag cag aag tgg aag cgc cag ccg gtc atc atg cca acc gtc att Val G u G n Lys Trp Lys Arg G n Pro Val Ile IVet Pro Thr Val Ile 35 ccg atg act gcg gaa acc acg cac atc ggt gac gat gag gtt cgc gct Pro Iet Thr Ala G u Thr Thr His Ile y Asp Asp G u Val Arg Ala 50 tca cgc gag tcc ctg taaaagcatt tcgcttttcg acg Ser Arg G u Ser Leu <210> <211> 63 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> IVet Asn Asp Leu Al a Al a G u G y u Asn Asp Pro Tyr Arg IVet Val 1 5 10 Sn G n Leu Arg Arg Lys Leu Ser Arg Phe Val G u G n Lys Trp Lys 25 Arg n Pro Val Ile Iet Pro Thr Val Ile Pro Iet Thr Ala u Thr 40 Page 113 161 209 257 295 seql i st cor r ect ed. t xt Thr His I I e y Asp Asp G u Val Arg Ala Ser Arg G u Ser Leu 55 <210> 71 <211> 4653 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(4630) <223> RXN00367 <400> 71 atcgaaagta acccttttgt tacttgcgtt gcaggtagtg gaacgattga tagaaacagg attaaagtga ggtatcccgc tccctgattt tcttattatc at g Met 1 aaa cca caa Lys Pro G n ctc tac aac cct Leu Tyr Asn Pro gcg Al a cat gaa cat gac His u His Asp gcc Al a 15 tgc ggt gtg gcg Cys Gy Val Ala ttt att Phe l e gcg gat at c Al a Asp I e gag gcg ctt G u Al a Leu cac ggt cga ccc agc cgc agc att gtt gat cgt gca ctt His Gy Arg Pro Ser Arg Ser lie Val Asp Arg Ala Leu 30 cgc aac att gac Arg Asn I e Asp cga ggt gcc gcc Arg y Ala Al a gca gag aag Al a G u Lys aac act Asn Thr ggc gat ggt gcg G y Asp G y Al a at c ctc atg cag lie Leu IVet n at t I e ccg gac ggc ttt Pro Asp y Phe t at Tyr cgt gaa gta tct Arg G u Val Ser att gag ctt cct Ile u Leu Pro gag Gu gca ggg gag tat Al a y u Tyr act ggt att gcg Thr G y I e Al a ttg cct cgc ggt Leu Pro Arg y atg gcg atg atg Mvt Ala MVt MVet gat gct Asp Al a 100 cag aag gaa G n Lys G u ggt tgg cgc G y Trp Ar g 120 gag cgc atc gca G u Arg lie Ala aag Lys 110 caa gaa ggt gcc Sn G u G y Al a gat gtg ctt Asp Val Leu 115 t cc at g gct Ser IVet Al a atg gtt cct ttt Met Val Pro Phe tct cgt gat ttg Ser Arg Asp Leu ggt Gy 130 gag gag G u G u 135 gcg atg cct agt Ala Met Pro Ser ttc Phe 140 gcg cag att ttc Ala n Ile Phe act gtg cct gga Thr Val Pro G y aaa Lys 150 tct ggt gaa gat Ser G y G u Asp gac cgt gtg atg Asp Arg Val IVet ttt atc cgt aag Phe lie Arg Lys tgt gag cgt gag ctg ggc acc acc aat ggt Cys G u Arg G u Leu G y Thr Thr Asn y 170 175 cgc gat acg gtg Arg Asp Thr Val tat ttc Tyr Phe 180 ccg tcg cta tct tca cgc acc atc att Pro Ser Leu Ser Ser Arg Thr lie lle tac aaa Tyr Lys Page 91 ggc atg ttg acc act G y IVet Leu Thr Thr seql i st cor r ect ed. t xt ct g Leu tcg Ser agc Ser 230 at c SIle ctt Leu tgc Cys ctt Leu at c SIle 310 gat Asp gca Al a cgt Ar g gtt Val gt c Val 390 act Thr agc Ser ct g Leu ctt Leu 200 at t SIle ccg Pr o act Thr aaa Lys ccg Pr o 280 cac Hi s cag G n t ac Tyr ct g Leu ggc Gy 360 at g Met aag Lys gag G u gcg Al a cgt Ar g 440 ggc Gy at t SIle gcg Al a cgt Ar g 250 gac Asp ggc Gy ggc Gy tgg Tr p t ac Tyr 330 ttt Phe cga Ar g tct Ser act Thr cgc Ar g 410 cca Pr o cct Pr o tt c Phe gt g Val cac Hi s 235 ggc Gy aag Lys tcg Ser gga Gy gaa G u 315 cac Hi s act Thr cct Pr o gaa G u cgc Ar g 395 at c SIle t at Tyr cag G n ttt Phe cac Hi s 220 ccg Pr o aat Asn ct g Leu gat Asp t ac Tyr 300 cac Hi s tct Ser gac Asp ggg Gy tcg Ser 380 gt a Val gtt Val ggt Gy aca Thr gag G u 205 tcg Ser t ac Tyr gaa G u ggc Gy acc Thr 285 t ca Ser aac Asn tgt Cys ggt Gy cga Ar g 365 gga Gy cag G n gaa G u gag G u cgc Ar g 445 190 gat Asp cgt Ar g cgt Ar g aac Asn aat Asn 270 gcg Al a ctt Leu aag Lys ct g Leu cgt Ar g 350 at c SIle gt g Val cct Pr o gac Asp tgg Tr p 430 t ac Tyr ggt Gy t cc Ser gtt Val 240 at g Met agc Ser ttc Phe cat Hi s ct g Leu 320 gag G u gt g Val at t SIle gac Asp cgc Ar g 400 gaa G u cgc Ar g t ac Tyr gat Asp acg Thr 225 gcc Al a cgc Ar g agc Ser gac Asp gct Al a 305 agc Ser cca Pr o ggt Gy act Thr ttg Leu 385 at g Met at c SIle gat Asp at g let gct Al a 210 aac Asn cac Hi s gcc Al a gt g Val gag G u 290 gtt Val cct Pr o tgg Tr p gcc Al a gat Asp 370 agg Ar g ttc Phe aag Lys aat Asn gcg Al a 450 cgc Ar g act Thr aac Asn cgc Ar g ct g Leu 275 gct Al a gcg Al a gag G u gat Asp gt g Val 355 tcg Ser gag G u ctt Leu cag G n ttt Phe 435 cac Hi s ct g Leu ttc Phe ggt Sy gag G u 260 cct Pr o ttg Leu at g let ct g Leu ggt G y 340 ct g Leu ggt G y gag G u gtt Val aaa Lys 420 gt g Val t ct Ser 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 gct gtg ttg cgt cag cgt gtt ttc gga atc act Ala Val Leu Arg n Arg Val Phe Oy I e Thr gaa gaa gat gtg gat G u u Asp Val Asp Page 92 seql i st cor r ect ed. t xt ttg Leu 470 at g Met ctt Leu ttg Leu ggt Sy at c SIle 550 at c SIle at t SIle gcg Al a aag Lys cct Pr o 630 cag G n gcc Al a gcg Al a aag Lys at c SIle 710 ct g Leu tcg Ser gat Asp tct Ser 520 cag G n ttg Leu gcc Al a ggt Sy gct Al a 600 ttg Leu cct Pr o cgt Ar g gag G u aac Asn 680 cag G n gca Al a ccg Pr o acg Thr 490 ttc Phe cgc Ar g gac Asp tcg Ser gcg Al a 570 t ac Tyr gt g Val gt g Val ct g Leu cgt Ar g 650 cat Hi s t ac Tyr ggt Gy acc Thr at g bet 475 cca Pr o gcg Al a gaa G u gt g Val ccg Pr o 555 cat Hi s cca Pr o cgc Ar g ct g Leu ct g Leu 635 acc Thr cac Hi s at g bet gat Asp act Thr 715 gcc Al a at t SIle cag G n aag Lys ct c Leu 540 at c SIle ggt G y gt g Val cgc Ar g tcg Ser 620 ct g Leu cag G n ct g Leu gca Al a ctt Leu 700 ggt Sy cag G n gcg Al a ttt Phe 510 gt g Val ccg Pr o gat Asp tgg Tr p cac Hi s 590 gtt Val cgt Ar g t cc Ser t cc Ser at g Met 670 gaa G u ttg Leu ct g Leu ggt G y ct a Leu 495 gct Al a acc Thr ggt Sy aac Asn gat Asp 575 cat Hi s tct Ser gaa G u gct Al a ct g Leu 655 ct c Leu acc Thr gat Asp aag Lys gct Al a 480 t cc Ser cag G n agc Ser cct Pr o cat Hi s 560 t cc Ser ggt G y gaa G u tct Ser gt g Val 640 gt g Val at t SIle at c SIle gag G u gt g Val 720 465 gag G u cag G n gt g Val at g Met gat Asp 545 gag G u ttt Phe gcc Al a gca Al a gat Asp 625 cat Hi s gt g Val ggt Sy gat Asp gca Al a 705 at g let gcg Al a cga Ar g aca Thr ttc Phe 530 gcg Al a ct g Leu ggt G y ggc G y at c l Ie 610 gag G u cag G n gaa G u ttt Phe gag G u 690 t cc Ser t cc Ser at t l Ie cca Pr o aac Asn 515 act Thr gcg Al a gcc Al a gct Al a at g let 595 cgc Ar g cgc Ar g t ac Tyr t cc Ser ggt Sy 675 ct g Leu cga Ar g aag Lys ggt G y cgc Ar g 500 cca Pr o ttg Leu cga Ar g acc Thr gct Al a 580 aag Lys aat Asn at g Met ttg Leu ggc Sy 660 gcc Al a cgc Ar g aac Asn at g Met 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 att gca acg gtg tct tcg tac cgt ggc gcg cag lie Ala Thr Val Ser Ser Tyr Arg y Ala G n ctt gcc gat gtc act Leu Al a Asp Val Thr Page 93 seql i st cor r ect ed. t xt 730 ggt Gy cca Pr o cgt Ar g gaa G u 790 cac Hi s tct Ser caa G n gac Asp gt g Val 870 gaa Gu t cc Ser ccc Pr o cgt Ar g at c SIle 950 cca Pr o ggc Gy cac Hi s tct Ser 760 cgc Ar g gat Asp ttc Phe agc Ser act Thr 840 aag Lys cgt Ar g cat Hi s t cc Ser ggt Gy 920 ggc Gy at g bet aag Lys ggt Gy cag gat ct c G n Asp Leu 745 ggc atc ggt G y lie y agc gca ttt Ser Al a Phe ttg ggt ggt Leu G y G y 795 aac cca gaa Asn Pro G u 810 t ac gag at t Tyr G u Ile 825 cgc ttg ggt Arg Leu G y cca att t cg Pro Ile Ser ttc tcc act Phe Ser Thr 875 gag gtc ttg G u Val Leu 890 ggc gaa ggt Gy Gu y 905 gac tgg aag Asp Trp Lys gtg acc agc Val Thr Ser gca cag ggc Al a G n y 955 gtg tac cca Val Tyr Pro 970 ctg att tcc Leu Ile Ser 985 ct g Leu ct g Leu ttg Leu 780 gaa G u acc Thr ttc Phe act Thr gt g Val 860 ggt Gy gcc Al a ggc Gy cgc Ar g cac Hi s 940 gca Al a tgg Tr p cct gac Asp gat Asp 765 cca Pr o t ac Tyr at c SIle aag Lys at t SIle 845 tct Ser gcg Al a at c l Ie gag G u tct Ser 925 t ac Tyr aag Lys gtt Val cca aac Asn 750 gaa G u cgc Ar g aag Lys ttc Phe gat Asp 830 cgt Ar g gag G u at g Met gcc Al a gac Asp 910 gcc Al a ttg Leu ccc Pr o gca Al a cca 735 tac ttc ggt Tyr Phe G y gtt gca gct Val Al a Al a cct gaa gag Pro G u G u 785 tgg cgc cgc Trp Arg Arg 800 aag ctg cag Lys Leu G n 815 tac acc cgc Tyr Thr Arg gga ctg ttt G y Leu Phe gtg gag ccg Val u Pro 865 tct tat ggc Ser Tyr G y 880 atg aac cga Ibet Asn Arg 895 gcc cgc cga Al a Ar g Ar g att aag cag Ile Lys G n aac aac tgc Asn Asn Cys 945 ggt gaa ggt Gy Gu y 960 gaa gtc cgc Su Val Arg 975 cac cac gat His His Asp ggt Gy gac Asp 770 cac Hi s gaa G u cat Hi s aag Lys gag G u 850 gt c Val tcg Ser ct g Leu ttt Phe gt g Val 930 acc Thr ggc Gy at c SIle at t at t SIle 755 gt a Val gct Al a ggt Gy gca Al a gtt Val 835 ttc Phe agt Ser at t SIle ggc Gy gat Asp 915 gcc Al a gat Asp cag G n acc Thr t ac gct Al a gaa G u cac Hi s gaa G u acg Thr 820 gat Asp agc Ser gag G u t ct Ser ggt Gy 900 gt g Val tcg Ser at t SIle ct g Leu acc Thr 980 t cc t ca Ser gct Al a cgt Ar g t ac Tyr 805 cgt Ar g gat Asp acg Thr at c SIe gct Al a 885 at g Met gaa G u gga Gy cag G n cca Pr o 965 cca Pr o at t 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 3091 Pro Pro Pro 990 Ile Tyr 995 Ser Ile gag gat ct g G u Asp Leu gct cag ctg atc cac gac ctg aag aac gct aac cca cgc 3139 Ala G n Leu lie His Asp Leu Lys Asn Ala Asn Pro Arg Page 94 seql i st cor r ect ed. t xt 1000 1005 1010 gca cga atc cac gtg aag cta gtg gca gaa caa ggc gtg ggc acc gtt 3187 Ala Arg Ile His Val Lys Leu Val Ala G u G n y Val G y Thr Val 1015 1020 1025 gcc gca ggt gtg tcc aaa gca cac gct gat gtg gtg ctt att tcc ggc 3235 Ala Al a y Val Ser Lys Ala His Ala Asp Val Val Leu le Ser y 1030 1035 1040 1045 cac gat ggc gga act ggc gca tct cct ttg acc tcc ctg aag cat gcc 3283 His Asp G y G y Thr G y Ala Ser Pro Leu Thr Ser Leu Lys His Ala 1050 1055 1060 ggt ggt cca tgg gag ttg ggc ttg gct gaa acc cag caa acg ttg ctg 3331 G y G y Pro Trp G u Leu G y Leu Ala G u Thr G n G n Thr Leu Leu 1065 1070 1075 ctc aac ggc ctg cgc gat cgt att cgc gtg cag tgc gat ggt cag ctg 3379 Leu Asn y Leu Arg Asp Arg Ile Arg Val G n Cys Asp Gy G n Leu 1080 1085 1090 aaa act ggc cga gac gtg gtt atc gca gct ctt ctc ggt gcc gaa gaa 3427 Lys Thr y Arg Asp Val Val Ile Ala Ala Leu Leu y Ala u u 1095 1100 1105 ttc ggt ttt gcc acc gca ccg ctg gtg gtt gaa ggc tgc atc atg atg 3475 Phe G y Phe Al a Thr Al a Pro Leu Val Val G u G y Cys Ile IVet Mt 1110 1115 1120 1125 cgc gtc tgc cac ctg gac acc tgc ccg gtg ggt atc gct acc cag aac 3523 Arg Val Cys His Leu Asp Thr Cys Pro Val Gy I e Ala Thr G n Asn 1130 1135 1140 ccg gat ttg cgt tcc aag ttc acc ggc aag gct gaa cac gtg gtc aac 3571 Pro Asp Leu Arg Ser Lys Phe Thr y Lys Ala G u His Val Val Asn 1145 1150 1155 ttc ttc acc ttc atc gcc cag gaa gtc cgt gag tac ttg gca cag ctt 3619 Phe Phe Thr Phe Ile Al a n u Val Arg G u Tyr Leu Ala G n Leu 1160 1165 1170 ggt ttc cgc tct att gat gaa gcc gtc gga caa gcc cag gtg ctg cgc 3667 Sy Phe Arg Ser Ile Asp G u Ala Val y G n Ala G n Val Leu Arg 1175 1180 1185 aag cgt tcc gga atc cca gct gat tcc cgc gca gca cac ctg gat ttg 3715 Lys Arg Ser y Ile Pro Ala Asp Ser Arg Ala Ala His Leu Asp Leu 1190 1195 1200 1205 agc cca att ttc cat cgc cca gaa act cca cac ttc cca act cag gat 3763 Ser Pro lie Phe His Arg Pro G u Thr Pro His Phe Pro Thr n Asp 1210 1215 1220 gtg cgt tgc acc aag acc cag gaa cac agc cta gaa aaa gcc ctg gac 3811 Val Arg Cys Thr Lys Thr n G u His Ser Leu G u Lys Ala Leu Asp 1225 1230 1235 aac gca ttt att gat aag gct tcg gac acg atc acc cgt gcc gca gcg 3859 Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile Thr Arg Al a Ala Al a 1240 1245 1250 ggt gtg gaa acc agc att gtt att gat agc tcc atc agc aac gtc aac 3907 Sy Val G u Thr Ser Ile Val lie Asp Ser Ser lie Ser Asn Val Asn 1255 1260 1265 cgt tca gtt ggc acg atg ctg ggt tct gca gtc agc cgc gtg gct ggt 3955 Arg Ser Val y Thr IVet Leu y Ser Ala Val Ser Arg Val Ala G y Page seqI i st car r ect ed. t xt 1280128 1270 1 275 1285 gcc caa ggt A a G3 n G3 y gcc ggt aac A a G3 y Asn ct C acc ggc Leu Thr G3 y 132C aag at t gt c Lys I Ie Val 1335 aat cca aat Asn Pr o Asr 1350 ggt gaa tt g a3 y a3 u Leu aac t ct ggc Asn Ser a31y gag t ac at g a3 u Tyr I'b t 140C aac t tt ggt Asn Phe a31 1415 ccg gac ct a Pro Asp LeL 1430 ct g agc gct Leu Ser A a cgc gaa ct C Ar g a31u Leu aaa at c at g Lys IlIe I'b t 148C gcc cac gct Ala Hi s Ale 1495 ttg cca Leu Pro 1290 t cc tt t Ser Phe 1305 gat gcc As p A a I at c aag I e Lys at c at t iI lIe I Ie t tc at t Phe I I e 1370 gcc acc Ala Thr 1385 act ggc Thr a3 y gcc ggc A a a3 y iaac cag i Asna(1n gac gat Asp Asp 1450 acc gga Thr a31y 1465 ccg cgc Pr o Ar g gag ggc ia31ua(1y gac ggc As p a3 y ggc gcg G3 y A a aat gac As n As p cct t cc Pr o Ser 1340 gcc gga Ala a31y 1 355 cgt ggc Ar g a31y gca gtg Ala Val ggc cga G31y Ar g at g t ct Mebt Ser 1420 aag at c Lys I Ie 1 435 ct g acg Leu Thr t cc gag Ser a3 u gat tt c As p Phe caa gac 31n As p 1 500 acc Thr I tt c Phe I t t Ph e 1325 gct A a aac As n cag gt t Val gt c ValI 1405 ggt a31y aat As n gg Tr p acc Thr caa 1485 cca Pr o at c Ile at c Ile 1310 gt g ValI c ag gt g ValI gt c a j aa 1390 ct g Le L ggC gct NI a aag Lys 1470 aaa Lys gca Aa ac c Thr 1295 cca Pr o a31y gct 1A a ct t Le u ggc a31y 1375 ggt a31y gt t Val at t I Ie gaa (31u g at As p 1455 ct g Le u gt a Val at c I Ie t tg aat ct t caa ggc tgc 4003 Leu Asn Leu (3 n cga Ar g aag Lys5 ccg Pr o gga a31y 1360 g aa a31u at c I Ie tt g Le u gca A a tt g Le u 1440 gag a31u cgt Ar g ct c Le u aag Lys5 ggc a31y gga a31y aag Lys5 1345 t ac Tyr cgt Ar g gga a31y a31y t ac Tyr 1425 gt g ValI ct c Leu gca A a aac As n at c I Ie 1 505 at c acc I I e Thr 1315 t ta t ct Leu Ser 1330 cag ct g a3 n Leu ggc gca a3 y A a t tc tg c Phe Cys aac cac As n Hi s 1395 ccg gtt Pr o Val 1410 ct g gct Leu A a Igat gt t As p Val at t gct I e A a caa gat a31n As p 1475 at c at c I Ie I Ie 1490 at g gag Ivbt (1u 1300 at c a31y aag Lys5 ac c Thr gt c ValI 1380 ggt a31y ggt a31y aat As n gt t ValI cgc Ar g 1460 tt g Le u g aa a31u gca A a Jy aac 4051 As n gga 4099 aac 4147 As n agt 4195 Ser 1 365 cgt 4243 Ar g tgt 4291 Cys gag 4339 cc 4387 Ser cca 4435 Pr a 1 445 cac 4483 Hi s gtg 4531 ValI acg 4579 Thr gtg 4627 ValI agc taatggccga cccacaagga ttc Ser 1510 <210> 72 <211> 1510 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 4653 Page 96 seqI i st car r ect ed. t xt <400> 72 I'bt Lys Pro O31n G31y ValI A a I Ie A a A a G31y Le u Le u 145 Ph e As p G31y As p Thr 225 A a Ar g Ser As p A a 305 Ser Pr o A a Ar g A a As p O31u vb t As p 115 Ser ValI Ar g ValI Le u 1 95 Ar g Thr As n Ar g Le u 275 A a A a O31u As p Gy le Le u Ly s Ph e A a A a Le u A a G31y Ar g 1 65 Ph e Thr O31u Pr o O31u 245 A a I Ie O31u vb t Ar g 325 Pr o Leu Tyr Asn Pro Ala His Ou His Asp AlaCys 10 A a O31u As n Tyr 70 Thr O31n G31y G u Lys5 150 Cy s Pr o Le u Ser Ser 230 li1e Le u Cy s Le u I Ie 310 As p A a As p A a Thr Ar g G31y Lys5 Tr p G u 135 Ser O31u Ser O31n A a 215 Tr p As n I Ie Thr Le u 295 Pr a Ph e A a I Ie Le u 40 G31y O31u I Ie O31u Ar g 120 A a G31y Ar g Le u Le u 200 li1e Pr a Thr Lys5 Pr a 280 Hi s O31n Tyr Le u Hi s Ar g As p ValI A a I Ie 1 05 vb t vb t O31u O31u Ser 1 85 O31u A a Le u ValI As n 265 O31u Le u A a O31u A a 345 Ar g I Ie A a O31y Le u Ar g Pr a Ser Le u 1 O31y Ar g Ph e ValI Hi s 235 O31y Lys5 Ser O31y O31u 315 Hi s Thr Ser Hi s li1e O31u Ar g A a As p 125 A a Ar g Thr I Ie O31u 205 Ser Tyr O31u O31y Thr 285 Ser As n Cys (31y Ar g Ar g Le u Le u O31y Lys5 110 Ser G n ValI As n I Ie 1 As p Ar g Ar g As n As n 270 A a Le u Ly s Le u Ar g 350 Ser O31y vb t Pr a Ar g O31n Ar g I Ie Nibt O31y 1 Tyr Le u Ph e Ph e Tr p 255 Le u Ar g Pr a Th r vb t 335 Ph e Oy Ala Val Leu Asp Arg Asn Oy Leu Arg Pro Oy Arg IlieThr Ilie Page 97 seqi i st car r ect ed. t xt Thr Le u 385 vb t I Ie As p vb t O31u 465 O31u O31n ValI vb t As p 545 O31u Ph e A a A a As p 625 Hi s ValI O31y As p A a 705 355 Ser (31u Le u O31n Ph e 435 Hi s As p I Ie Pr o As n 515 Thr A a A a A a vb t 595 Ar g Ar g Tyr Ser O31y 675 Le u 365 O31y Val Leu Asp Le u Ser As p 405 Le u Hi s Ar g As p Ser 485 vb t Pr o Le u Ar g Le u 565 ValI A a O31y A a ValI 645 As p As p vb t ValI ValI 390 Thr Ser Le u A a Le u 470 vb t Le u Le u O31y I Ie 550 li1e I Ie A a Ly s Pr o 630 O31n A a A a Ly s ValI 375 ValI A a O31u As p ValI 455 Le u G31y Tyr As p A a 535 Ar g As n Ser I Ie Thr 615 li1e O31n Ar g I Ie O31y 695 [Vbt A a Ser 03 u Ly s O31u A a Ar g 440 Le u Le u Ser As p Ser 520 O31n Le u A a O31y A a 600 Le u Pr o Ar g O31u As n 680 O31n Ar g O31y O31n 425 Le u Ar g Le u As p Ph e 505 li1e Ser O31u As n Le u 585 Ar g I Ie A a Thr ValI 665 Pr a Le u Ar g 395 li1e Tyr O31n Ar g vb t 475 Pr a A a O31u ValI Pr a 555 Hi s Pr a Ar g Le u Le u 635 Thr Hi s vb t As p Ser 380 ValI ValI O31y Thr ValI 460 A a I Ie O31n Ly s Le u 540 li1e O31y ValI Ar g Ser 620 Le u O31n Le u A a Le u 700 (31n O31u O31u Ar g 445 Ph e Ar g A a Ar g Pr a 525 As n I Ie O31u A a O31u 605 As p Thr cys A a Ph e 685 Ser (31y O31u 415 li1e As n I Ie O31y Le u 495 A a Thr O31y As n As p 575 Hi s Ser O31u A a Le u 655 Le u Th r As p Ser Ar g Asn Tyr I Ie Lys Ala Ala Thr Thr O31y Val Leu Lys Val Page 98 I'bt Ser Lys I'bt G31y 725 seqI i st car r ect ed. t xt IlieAla Thr Val Ser Ser Tyr Arg Gy 730 A a 03 n 735 Leu O31y A a G31ui 785 Ar g 31ni Ar g Phe Pr o 865 31y~ Ar g Ar gI 31n~ Cys~ 945 (31y Ar g I As p I As n (31y 1 025 Val AJ a (31y As p 770 HIi s (31u HIi s Lys (31u 850 ValI Se r e u Ph e ValI 930 Fh r J31y Ile Ile AJ a 101 C ValI e u As p I Ie 755 Val A aI (31y A a Val 835 Phe Ser I Ie (31y As p 915 A a As p 1 31ni Thr Ty r 995 As n (31y I Ie Val 740 AJ a (31u HIi s (31u Thr 820 As p Ser (31u Ser J31y 900 ValI Se r Ile e u Fh r 980 Se r Pr a Th r Se r Thr Ser A a Ar g Tyr 805 Ar g As p Thr I Ie A a 885 Pr a 965 Pr a Le u I Ie Hi s 775 Le u Le u (31y Ser Ar g 855 Ly s A a As n As n Ph e 935 Ly s As n ValI O31n 745 G31y Ser Le u As n Ty r 825 Ar g Pr a Ph e (31u G31y 905 As p ValI A a ValI Le u 985 Le u G31y Ph e G31y 795 G31u I Ie G31y Ser Th r 875 Le u G31y Ly s Ser G31y 955 Pr a Ser Le u Le u Le u 780 G31u Th r Ph e Th r ValI 860 G31y A a G31y Ar g Hi s 940 A a Tr p Pr a As p As p 765 Pr a Ty r I Ie Ly s I Ie 845 Ser A a I Ie G31u Ser 925 Ty r Ly s ValI Pr a Asn Tyr Phe 750 G31u Val Ala Ar g Pr o (31u Lys Tr p Ar g 800 Phe Lys Leu 815 Asp Tyr Thr 830 Ar g G31y Leu G31uVal (31u I'b t Ser Tyr 880 A a [Vb t Asn 895 Asp Ala Ar g 910 A a IlIe Lys Leu Asn Asn Pro G31y G31u 960 A a 03 u Val 975 Pr o Hi s Hi s 990 I I e 03 u Asp Leu 1 000 Ala O1n Leu Ilie His Asp Leu Lys 1 005 Ar g ValI A a A a 1 030 Hi s Ar g I Ie 1015 A a 03 y As p 31y( Hi s ValI Val Ser Th r Lys Leu Val A 1 020 Lys Ala Hi s AH 1 035 Gy Ala Ser Pro .31u *Asp Le u ValI 1 040 Th r 1045 1050 1055 Ser (31n Lys5 Th r 1 075 Hi s Ala (31y 1 060 Leu Leu Leu G31y Pr o Tr p O31u Leu G31y 1 065 As n (3y Leu Arg Asp Arg 1 080 Page 99 Leu Ala G31u 1 070 li1e Ar g Val 1 085 Th r Q n seql i st cor r ect ed. t xt Cys Asp Gy G n Leu Lys Thr 1090 1095 Sy Arg Asp Val Val Ile Ala Ala Leu 1100 Leu y Ala u u 1105 Sy Cys Ile Met Met 1125 lie Ala Thr n Asn 1140 G u His Val Val Asn 1155 Tyr Leu Ala G n Leu 1170 Ala G n Val Leu Arg 1185 Ala His Leu Asp Leu 1205 Phe Pro Thr G n Asp 1220 G u Lys Ala Leu Asp 1235 Thr Arg Ala Ala Al a 1250 I e Ser Asn Val Asn 1265 Ser Arg Val Ala y 1285 Asn Leu G n y Cys 1300 Sy Ile Thr Ile Asn 1315 Sy Leu Ser y y 1330 Lys G n Leu Lys Asn 1345 Tyr y Ala Thr Ser 1365 Arg Phe Cys Val Arg 1380 G y Asn His G y Cys 1395 G y Pro Val G y G u 1410 Tyr Leu Ala Asn Ser 1425 Phe y Phe Ala Thr 1110 Arg Val Cys His Leu 1130 Pro Asp Leu Arg Ser 1145 Phe Phe Thr Phe Ile 1160 Sy Phe Arg Ser lie 1175 Lys Arg Ser Oy Ile 1190 Ser Pro Ile Phe His 1210 Val Arg Cys Thr Lys 1225 Asn Ala Phe Ile Asp 1240 Sy Val G u Thr Ser 1255 Arg Ser Val G y Thr 1270 Ala G n y Leu Pro 1290 Ala y Asn Ser Phe 1305 Leu Thr y Asp Al a 1320 Lys Ile Val lie Lys 1335 Asn Pro Asn Ile Ile 1350 G y u Leu Phe Ile 1370 Asn Ser y Ala Thr 1385 G u Tyr lVbt Thr G y 1400 Asn Phe y Ala y 1415 Pro Asp Leu Asn G n 1430 Al a Pro Leu Val Val 1115 Asp Thr Cys Pro Val 1135 Lys Phe Thr G y Lys 1150 Ala G n u Val Arg 1165 Asp u Ala Val y 1180 Pro Ala Asp Ser Arg 1195 Arg Pro u Thr Pro 1215 Thr G n G u His Ser 1230 Lys Al a Ser Asp Thr 1245 I e Val Ile Asp Ser 1260 bet Leu y Ser Ala 1275 Asp y Thr Ile Thr 1295 Sy Ala Phe IIe Pr o 1310 Asn Asp Phe Val y 1325 Pro Ser Ala G n Al a 1340 Ala y Asn Val Leu 1355 Arg y n Val y 1375 Ala Val Val G u y 1390 G y Arg Val Leu Val 1405 lVbt Ser G y G y Ile 1420 Lys I e Asn y u 1435 u 1120 G y Al a G u G n Al a 1200 Hi s Leu Ile Ser Val 1280 Leu Arg Lys Pr o G y 1360 G u Ile Leu Al a Leu 1440 Val Asp Val Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp G u Page 100 seqI i st car r ect ed. t xt 1445 1450 1455 Leu Ilie Ala Arg Hi s Ar g O31u Leu Thr G31y Ser O31u Thr Lys Leu Arg 1460 1465 1470 Ala (31n As p Leu Val Lys I Ie N/bt Pr o Ar g Asp Phe O31n Lys Val Leu 1475 1480 1485 Asn I Ie Ile (1u Thr Ala Hi s Aia (1u (1y (1n As p ProAlaIle Lys 1490 1495 1500 Ilie Nib t 31u Ala Val Ser 1505 1510 <210> 73 <211> 1906 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> OIDS <222> (1906) <223> FRXA00007 <400> 73 at cgaaagt a accctt t tgt t act tgcgt t gcaggt agt g t ccct gat tt t ct tat tat c gaacgat tga t agaaacagg at taaagt ga ggt at cccgc ct c Le u gcg A a gag O31u aac As n t at Tyr act Thr c ag O31n ggt G31y gag (31u aac As n at c I Ie ct t Le u ggc G31y g aa (31u at t I Ie g aa (31u cgc Ar g 120 gcg A a gcg A a ggt G31y aac As n ggt G31y t ct Ser tt c Ph e gag (31u gt t ValI cct Pr o cat Hi s cga Ar g at t I Ie gcg A a ggc G31y 75 t t g Le u cgc Ar g cct Pr o ag t Ser g aa (31u ccc Pr o g ac As p ggc G31y at t I Ie cct Pr o at c I Ie tt t Ph e tt c Ph e 140 cat Hi s ag c Ser c ac Hi s 45 at c I Ie gag (31u cgc Ar g gca A a g at As p 125 gcg A a g ac As p cgc Ar g cga Ar g ct c Le u ct t Le u ggt G31y aag Lys5 110 t ct Ser c ag O31n gcc A a 15 ag c Ser ggt G31y at g vb t cct Pr a cgc Ar g 95 c aa O31n cgt Ar g at t I Ie t gc cys at t I Ie gcc A a c ag O31n gag O31u 80 at g vb t g aa O31u g at As p tt c Ph e at g vb t 1 ggt O31y gt t ValI gcc A a at t I Ie gca A a gcg A a ggt O31y tt g Le u ct t Le u 145 gt g ValI g at As p ggt O31y ccg Pr a ggg O31y at g vb t gcc A a ggt O31y 130 act Thr gcg A a cgt Ar g gca A a g ac As p gag O31u at g Nibt g at As p 115 t cc Ser gt g ValI tt t Ph e 20 gca A a gag O31u O31y t at Tyr g at As p 1 00 gt g ValI at g vb t cct Pr a aaa cca caa Lys Pro O31n gga (31y at t I Ie ct t Le u aag tt t Ph e gcc A a gct A a ct t Le u gct A a gga (31y Page 101 s aaa t ct ggt gaa gat ctt gac cgt Lys Ser Gy G u Asp Leu Asp Arg 150 155 eql i st cor r ect ed. t xt gtg atg ttc ttt atc Val bet Phe Phe lie tgt Cys ccg Pr o ct g Leu tcg Ser agc Ser 230 at c SIle ctt Leu tgc Cys ctt Leu at c SIle 310 gat Asp gca Al a cgt Ar g gtt Val gt c Val 390 act Thr cgt Ar g ct a Leu ctt Leu 200 at t SIle ccg Pr o act Thr aaa Lys ccg Pr o 280 cac Hi s cag G n t ac Tyr ct g Leu ggc Gy 360 at g Met aag Lys gag Gu ct g Leu 170 t ca Ser ggc Gy at t SIle gcg Al a cgt Ar g 250 gac Asp ggc Gy ggc Gy tgg Tr p t ac Tyr 330 ttt Phe cga Ar g tct Ser act Thr cgc Ar g 410 ggc Gy cgc Ar g tt c Phe gt g Val cac Hi s 235 ggc Gy aag Lys tcg Ser gga Gy gaa G u 315 cac Hi s act Thr cct Pr o gaa Gu cgc Ar g 395 at c SIle acc Thr acc Thr ttt Phe cac Hi s 220 ccg Pr o aat Asn ct g Leu gat Asp t ac Tyr 300 cac Hi s tct Ser gac Asp ggg Gy tcg Ser 380 gt a Val gtt Val acc Thr at c SIle gag Gu 205 tcg Ser t ac Tyr gaa Gu ggc Gy acc Thr 285 t ca Ser aac Asn tgt Cys ggt Gy cga Ar g 365 gga Gy cag G n gaa G u aat Asn at t SIle 190 gat Asp cgt Ar g cgt Ar g aac Asn aat Asn 270 gcg Al a ctt Leu aag Lys ct g Leu cgt Ar g 350 at c SIle gt g Val cct Pr o gac Asp ggt Gy 175 t ac Tyr ct g Leu ttc Phe ttc Phe tgg Tr p 255 ttg Leu cgt Ar g ccg Pr o acg Thr at g Met 335 ttt Phe acc Thr ttg Leu gga Gy gag 15u 415 160 cgc Ar g aaa Lys ggt Gy t cc Ser gtt Val 240 at g Met agc Ser ttc Phe cat Hi s ct g Leu 320 gag G u gt g Val at t SIle gac Asp cgc Ar g 400 gaa G u gat Asp ggc Gy gat Asp acg Thr 225 gcc Al a cgc Ar g agc Ser gac Asp gct Al a 305 agc Ser cca Pr o ggt Gy act Thr ttg Leu 385 at g Met at c SIle acg Thr at g Met gct Al a 210 aac Asn cac Hi s gcc Al a gt g Val gag G u 290 gtt Val cct Pr o tgg Tr p gcc Al a gat Asp 370 agg Ar g ttc Phe aag Lys cgt aag cgt Arg Lys Arg 165 gtg tat ttc Val Tyr Phe 180 ttg acc act Leu Thr Thr 195 cgc ctg gag Arg Leu G u act ttc cca Thr Phe Pro aac ggt gag Asn G y u 245 cgc gag gcg Arg G u Ala 260 ctg cct at c Leu Pro Ile 275 gct ttg gag Al a Leu G u gcg atg atg Al a vebt Vet gag ctg cgt G u Leu Arg 325 gat ggt cct Asp Gy Pro 340 gtg ctg gac Val Leu Asp 355 tcg ggt ttg Ser G y Leu gag gag agc G u u Ser ctt gtt gac Leu Val Asp 405 cag aaa tta G n Lys Leu 420 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 Page 102 seql i st cor r ect ed. t xt agc gaa gcg cag cca tat ggt gag Ser u Ala n Pro Tyr Gy u 425 tgg Tr p 430 att cgc gat aat ttt gtg cat lie Arg Asp Asn Phe Val His ct g Leu gct Al a ttg Leu 470 at g Met ctt Leu ttg Leu ggt Gy at t SIle 550 at c SIle at t SIle gcg Al a cgt ct g cct Arg Leu Pro 440 ttg cgt cag Leu Arg G n ctg ctg ccg Leu Leu Pro t cg gat acg Ser Asp Thr 490 gat ttc ttc Asp Phe Phe 505 tct atc cgc Ser I e Ar g 520 cag t ct gac G n Ser Asp ttg gaa tcg Leu G u Ser gcc aac gcg Al a Asn Al a 570 ggt ttg tac G y Leu Tyr 585 gct cgt gtg Al a Ar g Val 600 cag aca cgc tac aac G n Thr Arg Tyr Asn 445 cgt gtt ttc gga atc Arg Val Phe Gy Ile 460 atg gcc cgc cag ggt IVet Ala Arg n y 475 cca att gcg gcg cta Pro Ile Ala Ala Leu 495 gcg cag cgc ttt gct Al a G n Arg Phe Ala 510 gaa aag cct gtg acc G u Lys Pro Val Thr 525 gtg ctc aat ccg ggt Val Leu Asn Pro y 540 ccg atc att gat aac Pro Ile Ile Asp Asn 555 cat ggt gag tgg gat His G y G u Trp Asp 575 cca gtg gct cac cat Pro Val Ala His His 590 t ac Tyr act Thr gct Al a 480 t cc Ser cag G n agc Ser cct Pr o cat Hi s 560 t cc Ser at g NMt gaa G u 465 gag G u cag G n gt g Val at g Met gat Asp 545 gag G u ttt Phe gcg Al a 450 gaa G u gcg Al a cga Ar g aca Thr ttc Phe 530 gcg Al a ct g Leu ggt Sy 435 cac Hi s gat Asp at t l Ie cca Pr o aac Asn 515 act Thr gcg Al a gcc Al a gct Al a t ct Ser gt g Val ggt G y cgc Ar g 500 cca Pr o ttg Leu cga Ar g acc Thr gct Al a 580 cgt Ar g gat Asp t cc Ser 485 at g Met ccg Pr o ttg Leu cgt Ar g ttg Leu 565 gt a Val 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1906 ggt gcc ggc atg aag gct Sy Ala Gy bet Lys Ala 595 <210> 74 <211> 602 <212> PRT <213> Corynebacter i um gl utam cum <400> 74 IVet Lys Pro G n y Leu Tyr Asn Pro 1 5 G y Val Ala Phe I Ie Ala Asp lie His 25 Val Asp Arg Ala Leu G u Ala Leu Arg 40 Al a y Al a G u Lys Asn Thr G y Asp 55 lie Pro Asp y Phe Tyr Arg u Val Ala His u His Asp Ala Cys 10 Sy Arg Pro Ser Arg Ser lie Asn I I e Asp His Arg G y Ala Sy Ala y lie Leu IVet n Ser y Ile u Leu Pro u Page 103 seqi i st car r ect ed. t xt Th r 03 y I I e A a Leu Pro Ar g G31y A a A a G31y Le u Le u 145 Ph e As p G31y As p Thr 225 A a Ar g Ser As p A a 305 Ser Pr o G31y Thr Le u 385 vb t I Ie O31u As p 115 Ser ValI Ar g ValI Le u 1 95 Ar g Thr As n Ar g Le u 275 A a A a O31u As p ValI 355 Ser O31u Le u O31n A a A a Le u A a G31y Ar g 1 65 Ph e Thr (31u Pr o (31u 245 A a I Ie (31u vb t Ar g 325 Pr o As p Le u Ser As p 405 Le u Ly s Tr p O31u 135 Ser O31u Ser O31n A a 215 Tr p As n I Ie Thr Le u 295 Pr o Ph e A a As n ValI 375 ValI A a O31u I Ie 1 vb t vb t O31u O31u Ser 1 O31u A a Le u ValI As n 265 O31u Le u A a O31u A a 345 Le u A a Ar g G31y O31n 425 Ar g Pr a Ser Le u 1 G31y Ar g Ph e ValI Hi s 235 G31y Ly s Ser G31y O31u 315 Hi s Thr Pr a O31u Ar g 395 li1e Tyr A a As p 125 A a Ar g Thr I Ie O31u 205 Ser Tyr O31u G31y Thr 285 Ser As n cys G31y Ar g 365 G31y O31n O31u O31u Ar g (31n Ar g I Ie vb t O31y 1 Tyr Le u Ph e Ph e Tr p 255 Le u Ar g Pr a Thr vb t 335 Ph e Th r Le u O31y O31u 415 li1e Page 104 seql i st cor r ect ed. t xt Asp Asn Phe Val His Leu Asp Arg Leu Pro G n Thr Arg Tyr Asn Tyr 435 440 445 IVet Ala His Ser Arg Ala Val Leu Arg G n Arg Val Phe G y I le Thr 450 455 460 G u G u Asp Val Asp Leu Leu Leu Leu Pro IVet Ala Arg G n y Ala 465 470 475 480 Su Ala Ile y Ser IVet y Ser Asp Thr Pro Ile Ala Ala Leu Ser 485 490 495 G n Arg Pro Arg IVet Leu Tyr Asp Phe Phe Ala G n Arg Phe Ala G n 500 505 510 Val Thr Asn Pro Pro Leu Asp Ser Ile Arg G u Lys Pro Val Thr Ser 515 520 525 IVet Phe Thr Leu Leu G y Al a n Ser Asp Val Leu Asn Pro G y Pro 530 535 540 Asp Ala Ala Arg Arg lle Arg Leu u Ser Pro lie lie Asp Asn His 545 550 555 560 G u Leu Ala Thr Leu I e Asn Ala Asn Ala His G y G u Trp Asp Ser 565 570 575 Phe G y Ala Ala Val I e Ser G y Leu Tyr Pro Val Ala His His G y 580 585 590 Ala Gy IVet Lys Ala Ala Ile Ala Arg Val 595 600 <210> <211> 1362 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (70)..(1362) <223> FRXA00364 <400> accaatttct ggcatcggtc tggatgaagt tgcagctgac gtagaaagct cgtcaccgca gcgcatttt ttg cca cgc cct gaa gag cac gct cac cgt gaa ttg gat ttg 111 Leu Pro Arg Pro u u His Ala His Arg u Leu Asp Leu 1 5 ggt ggt gaa tac aag tgg cgc cgc gaa ggt gaa tac cac ctg ttc aac 159 G y G y G u Tyr Lys Trp Arg Arg G u G y G u Tyr His Leu Phe Asn 20 25 cca gaa acc atc ttc aag ctg cag cat gca acg cgt tct ggc agc tac 207 Pro G u Thr IIe Phe Lys Leu G n His Ala Thr Arg Ser G y Ser Tyr 40 gag att ttc aag gat tac acc cgc aag gtt gat gat caa tcc act cgc 255 G u Ile Phe Lys Asp Tyr Thr Arg Lys Val Asp Asp G n Ser Thr Arg 55 ttg ggt act att cgt gga ctg ttt gag ttc agc acg gac cgc aag cca 303 Leu y Thr lie Arg G y Leu Phe G u Phe Ser Thr Asp Arg Lys Pro 70 att tcg gtg tct gag gt g gag ccg gtc agt gag atc gtg aag cgt ttc 351 Page 105 seql i st cor r ect ed. t xt lie Ser Val Ser u Val u Pro Val Ser u Ile Val 85 Lys Arg Phe t cc Ser gt c Val gaa G u tgg Tr p acc Thr cag G n 175 t ac Tyr at t SIle cag G n gt g Val t cc Ser 255 act Thr gag G u cgc Ar g gac Asp acc Thr 335 ggt Sy gcc Al a ggc G y cgc Ar g 145 cac Hi s gca Al a tgg Tr p cct Pr o at c SIle 225 ct a Leu gca Al a gca Al a ggc G y cgt Ar g 305 gtt Val ccg Pr o at g Met gcc Al a 115 gac Asp gcc Al a ttg Leu ccc Pr o gca Al a 195 cca Pr o gac Asp gca Al a gct Al a cct Pr o 275 gct Al a cgc Ar g gca Al a gt g Val tct Ser 100 at g Met gcc Al a at t l Ie aac Asn ggt G y 180 gaa G u cac Hi s ct g Leu gaa G u gat Asp 260 tt g Leu gaa G u gt g Val gct Al a gtt Val 340 t at Tyr aac Asn cgc Ar g aag Lys aac Asn 165 gaa G u gt c Val cac Hi s aag Lys caa G n 245 gt g Val acc Thr acc Thr cag G n ctt Leu 325 gaa G u ggc G y cga Ar g cga Ar g cag G n 150 tgc Cys ggt Gy cgc Ar g gat Asp aac Asn 230 ggc G y gt g Val t cc Ser cag G n tgc Cys 310 ct c Leu ggc G y tcg Ser ct g Leu ttt Phe 135 gt g Val acc Thr ggc G y at c SIle at t SIle 215 gct Al a gt g Val ctt Leu ct g Leu caa G n 295 gat Asp ggt G y tgc Cys tct Ser 105 ggt G y gt g Val tcg Ser at t SIle ct g Leu 185 acc Thr t cc Ser cca Pr o acc Thr t cc Ser 265 cat Hi s ttg Leu cag G n gaa G u at g Met 345 gct Al a at g Met gaa G u gga G y cag G n 170 cca Pr o cca Pr o at t SIle cgc Ar g gtt Val 250 ggc G y gcc Al a ct g Leu ct g Leu gaa G u 330 at g Met gaa G u t cc Ser ccc Pr o cgt Ar g 155 at c SIle cca Pr o ggc G y gag G u gca Al a 235 gcc Al a cac Hi s ggt G y ct c Leu aaa Lys 315 ttc Phe cgc Ar g gcc Al a aac Asn aac Asn 140 ttc Phe aag Lys aac Asn gtt Val gat Asp 220 cga Ar g gca Al a gat Asp ggt G y aac Asn 300 act Thr ggt G y gt c Val cat Hi s t cc Ser 125 ggt G y ggc G y at g Met aag Lys ggt G y 205 ct g Leu at c SIle ggt G y ggc G y cca Pr o 285 ggc G y ggc G y ttt Phe tgc Cys gag G u 110 ggc G y gac Asp gt g Val gca Al a gt g Val 190 ct g Leu gct Al a cac Hi s gt g Val gga G y 270 tgg Tr p ct g Leu cga Ar g gcc Al a cac Hi s 350 399 447 495 543 591 639 687 735 783 831 879 927 975 1023 1071 1119 1167 ctg gac acc tgc ccg gtg ggt atc gct acc cag aac ccg gat ttg cgt Page 106 Le u t cc Ser at c I Ie at t I Ie at c I Ie 415 at c I Ie Thr tt c Ph e c ag O31n 385 g aa O31u gct A a Pr o 355 ggC G31y gt c ValI gt c ValI t cc Ser ValI aag Ly s cgt Ar g gga G31y cgc Ar g 420 G31y gct A a gag O31u c aa O31n 405 gca A a seqI i st car r ect ed. t xt I Ie Ala Thr O31n As n Pro 360 gaa cac gt g gt c aac tt c O31u Hi s Val Val As n Phe 375 t ac t tg gca cag ct t ggt Ty r Leu Ala O31n Leu G31y 390 395 gcc cag gt g ct g cgc aag Ala O31n Val Leu Ar g Lys 410 gca cac ct g gat t tg agc A a Hi s Leu Asp Leu Ser 425 As p tt c Ph e 380 tt c Ph e cgt Ar g cca Pr o Le u 365 ac c Thr cgc Ar g t cc Ser at t I Ie 1215 1263 1311 1359 1362 <210O> 76 <21 1> 431 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 76 Le u O31u Th r Ph e Th r ValI O31y A a O31y Ar g 145 Hi s A a Tr p Pro Arg Pr o Ty r Lys Tr p 1 1 e Phe Lys Lys Asp Tyr Ilie Ar g O31y Ser 03 u Val A a I'b t Ser 1 00 Ile A a I'b t 115 O3 u Asp A a 130 Ser A a Il e Tyr Leu Asn Lys Pr o (31y 1 80 Val Ala (3u 1 95 O31u Ar g Le u Thr Le u O31u Tyr As n Ar g Lys5 As n 1 65 O31u ValI 03 u Hi s A a Hi s O31u Hi s Lys5 55 O31u ValI Ser Le u Ph e 135 ValI Thr O31y I Ie O31y A a 40 ValI Ph e Ser I Ie O31y 120 As p A a As p O31n Thr 200 O31u 25 Thr As p Ser O31u Ser 1 05 O31y ValI Ser I Ie Le u 1 85 Thr Ar g O31u Leu 10 Ty r Hi s Leu Ar g Ser O31y As p 03 n Ser Thr As p Ar g li1e Val Lys 90 A a 03 u A a I'b t Ser As n O31u Pr o As n 140 O31y Ar g Phe 1 O3 n I I e Lys 1 70 Pr o Pr o As n Pr o (3y Val As p Ph e Ser Thr Lys5 Ar g Hi s Ser 125 O31y O31y vb t Lys5 (31y 205 Le u As n Tyr Ar g Pr o Ph e O31u 110 O31y As p ValI A a ValI 1 O31y Pr a O31u Le u I Ie Ser ValI O31u Tr p Th r O31n 1 Ty r Leu I I e Ser Page 107 seqI i st car r ect ed. t xt Pro Pro Pro His His Asp IlieTyr Ser 210 215 I I e G3 u Asp 220 Leu Al a G n Leu I Ie 225 Le u Al a Al a O31y Ar g 305 ValI Pr o Thr Ph e O31n 385 O31u Al a As p Al a Al a Pr o 275 Al a Ar g Al a ValI Pr o 355 O31y ValI ValI Ser Lys5 O31n 245 ValI Thr Thr O31n Le u 325 G u O31y Al a (31u O31n 405 Al a ValI Le u Le u O31n 295 As p O31y cys Al a Hi s 375 Le u O31n As n O31y I Ie Lys5 280 Thr O31y Al a I Ie Thr 360 ValI Al a ValI Pr o Thr Ser 265 Hi s Le u O31n O31u vb t 345 O31n ValI O31n Le u As p 425 Ar g ValI 250 O31y Al a Le u Le u O31u 330 vb t As n As n Le u Ar g 410 Al a 235 Al a Hi s O31y Le u Lys5 315 Ph e Ar g Pr a Ph e O31y 395 Lys5 O31y O31y Pr a 285 O31y O31y Ph e cys Le u 365 Thr Ar g Ser ValI Ser 255 Thr O31u Ar g As p Thr 335 Le u Ser le le le 415 Al aAl a Hi s Leu Leu Ser Pro Ilie Phe Ilie <210O> 77 <21 1> 866 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (843) <223> FRXA00367 <400> 77 cac agc cta gaa aaa gcc ct g gac aac gca t tt at t gat aag gct t cg Hi s Ser Leu O31u Lys Ala Leu Asp Asn Al a Phe Ilie Asp Lys Al a Ser 1 5 10 gac acg at c acc cgt gcc gca gcg ggt gt g gaa acc agc at t gt t at t Asp Thr Ilie Thr Ar g Al a Ala Ala O31y Val O31u Th r Ser Ilie Val Ilie 25 gat agc t cc at c agc aac gtc aac cgt tca gt t ggc acg at g ct g ggt Asp Ser Ser Ilie Ser Asn Val Asn Ar g Ser Va 1O31y Thr I'b t Leu O31y 40 tct gca gtc agc cgc gtg gct ggt gcc caa ggt ttg cca gac ggc acc Page 108 Ser Ala Val Ser Arg Val Ala 55 G seql i st cor r ect ed. t xt y Ala Gn Gy Leu Pro Asp G y Thr at c I e at c SIle gt g Val cag G n gt g Val gt c Val 145 gaa G u ct g Leu ggc G y ggc G y gct Al a 225 aag Lys aaa Lys gca Al a ttc ttg Leu cga Ar g aag Lys ccg Pr o 115 gga Gy gaa G u at c SIle ttg Leu gca Al a 195 ttg Leu gag G u cgt Ar g ct c Leu aag Lys 275 ctt Leu at c SIle tta Leu cag G n ggc Gy ttc Phe aac Asn 165 ccg Pr o ct g Leu gat Asp at t SIle caa G n 245 at c SIle caa G n 70 acc Thr tct Ser ct g Leu gca Al a tgc Cys 150 cac Hi s gtt Val gct Al a gtt Val gct Al a 230 gat Asp at c SIle ggc G y at c SIle ggc Gy aag Lys acc Thr 135 gt c Val ggt G y ggt G y aat Asn gtt Val 215 cgc Ar g ttg Leu gaa G u gcc Al a ct c Leu aag Lys 105 aat Asn ggt Gy aac Asn gag G u aac Asn 185 ccg Pr o ct g Leu cgc Ar g aaa Lys gcc Al a 265 ggt aac Sy Asn 75 acc ggc Thr G y 90 att gtg Ile Val cca aat Pro Asn gaa ttg G u Leu tct ggc Ser G y 155 tac at g Tyr 'bet 170 ttt ggt Phe G y gac ct a Asp Leu agc gct Ser Al a gaa ct c G u Leu 235 at c at g I e bet 250 cac gct Hi s Al a t cc Ser gat Asp at c SIle at c SIle ttc Phe 140 gcc Al a act Thr gcc Al a aac Asn gac Asp 220 acc Thr ccg Pr o gag G u ttt Phe gcc Al a aag Lys at t SIle 125 at t SIle acc Thr ggc G y ggc G y cag G n 205 gat Asp gga G y cgc Ar g ggc G y ggc G y aat Asn cct Pr o 110 gcc Al a cgt Ar g gca Al a ggc G y at g Met 190 aag Lys ct g Leu t cc Ser gat Asp caa G n 270 gcg Al a gac Asp t cc Ser gga G y ggc G y gt g Val cga Ar g 175 tct Ser at c SIle acg Thr gag G u ttc Phe 255 gac Asp ttc Phe ttt Phe gct Al a aac Asn cag G n gtt Val 160 gtc Val ggt G y aat Asn tgg Tr p acc Thr 240 caa G n cca Pr o atc atg gag gca Ile Vet Ou Ala gtg agc Val Ser 280 taatggccga cccacaagga <210> 78 <211> 281 <212> PRT <213> Corynebacteri um gl utami cum <400> 78 Page 109 seqi i st car r ect ed. t xt Hi s Ser Leu O31u Lys Ala Leu Asp Asn Ala Phe I Ie Asp 1 5 10 Lys A a Ser As p As p Ser lIe lie ValI O31n ValI ValI 145 O31u Le u G31y G31y A a 225 A a I Ie Ser ValI Le u Ar g Lys5 Pr o 115 G31y O31u I Ie Le u A a 1 95 Le u O31u Ar g Le u Lys5 275 Ar g Ser Ar g Le u I Ie Le u O31n O31y Ph e As n 1 65 Pr o Le u As p I Ie O31n 245 li1e b t A a As n ValI O31n 70 Thr Ser Le u A a Cy s 150 Hi s ValI A a ValI A a 230 As p I Ie aOu A a ValI A a O31y I Ie O31y Lys5 Thr 135 ValI O31y O31y As n ValI 215 Ar g Le u O31u A a A a As n 40 O31y cys As n O31y As n 120 Ser Ar g cys O31u Ser 200 Pr o Hi s ValI Thr ValI 280 O31y Ar g A a A a Le u Lys5 1 05 As n O31y As n O31u As n 1 85 Pr o Le u Ar g Lys5 A a 265 Ser O31u ValI O31y As n O31y ValI As n Le u O31y 1 vb t O31y Le u A a Le u 235 vb t A a Thr O31y Le u Ser As p I Ie I Ie Ph e 140 A a Thr A a As n As p 220 Thr Pr a O31u Ser Thr Pr a Ph e A a Lys5 I Ie 125 li1e Thr O31y O31y O31n 205 As p O31y Ar g O31y lIe vb t As p O31y As n Pr a 110 A a Ar g A a O31y 1 Lys5 Le u Ser As p O31n 270 ValI Le u O31y A a As p Ser O31y O31y ValI Ar g 1 Ser I Ie Thr O31u Ph e 255 As p <210O> 79 <21 1> 1494 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1471) <223> RXN00076 <400> 79 t ct aggagt g t taaacagcc t ggact tgaa acacct t taa ct act tgat t t tcacaccct Page 110 seql i st cor r ect ed. t xt tgtttccata aaagggctca cgaaaggcaa cttcaaacac cgc Ar g ct c Leu caa G n cac Hi s aag Lys acc Thr ggc Gy ggc Gy cgc Ar g ggc Gy 150 ggc Gy ct c Leu cca Pr o t cc Ser ggc Gy 230 tgc Cys gcc Al a at c SIle cct Pr o cgc Ar g cgc Ar g gaa Gu gtt Val ttc Phe 120 gag G u ggt Gy gag G u gaa G u cag G n 200 acc Thr tct Ser at c SIle at c I e aat Asn ccg Pr o aag Lys cgc Ar g ct c Leu gac Asp gcc Al a t cc Ser gt c Val aaa Lys 170 aag Lys aag Lys aac Asn gaa G u gaa G0u 250 gga Gy gaa G u tt c Phe ggc G y ct g Leu 75 cgc Ar g cgc Ar g ggc Sy tgg Tr p ggc Sy 155 gt c Val gcc Al a tt c Phe gt g Val gcc Al a 235 cag G n gct Al a gag G u gga Gy at c lle ct c Leu gac Asp gac Asp gag G u gat Asp 140 ct c Leu acc Thr act Thr acc Thr gtt Val 220 cgc Ar g t ac Tyr ggc Gy cgc Ar g ct c Leu 45 gtt Val ggt Gy t ac Tyr ct c Leu ttc Phe 125 ct g Leu gac Asp gaa G u gaa G u cca Pr o 205 gtt Val cgc Ar g gca Al a cct Pr o gaa G u at c SIle aag Lys aac Asn t ac Tyr aac Asn 110 gtt Val tct Ser gt a Val at t SIle gt g Val 190 cag G n gat Asp gca Al a at c l Ie gct Al a 15 gt g Val cgt Ar g t cc Ser at t SIle gat Asp 95 at c SIle ggc Sy gca Al a gcc Al a t cc Ser 175 cac Hi s gaa G u cca Pr o t cc Ser cgc Ar g 255 ggc Gy ttc Phe t ac Tyr ct g Leu gaa G u gca Al a ccc Pr o ttc Phe cag G n cgc Ar g 160 gac Asp gtt Val ct c Leu gaa G u aag Lys 240 gag G u at g Met 1 at t SIle gtt Val ggc G y cac Hi s at c SIle gt c Val gga Sy t ac Tyr t cc Ser 145 at c SIle aac Asn ttc Phe aaa Lys gac Asp 225 t cc Ser cca Pr o t ac Tyr gac Asp gtt Val aac Asn ggc G y gt g Val at t SIle gac Asp 130 gt c Val ct g Leu gt c Val gga G y gaa G u 210 at c SIle cag G n aag Lys gca Al a ctt Leu gct Al a gt g Val aaa Lys ttc Phe gaa G u 115 ggc Sy gct Al a gct Al a t ac Tyr cgt Ar g 195 ct c Leu gac Asp gac Asp gac Asp t cc Ser ttc Phe cca Pr o ttg Leu gac Asp t cc Ser 100 gca Al a aac Asn gtt Val aag Lys gac Asp 180 cgt Ar g gac Asp t ac Tyr ctg Leu gct Al a 260 aca act ccc Thr Thr Pro Page 111 seql i st cor r ect ed. t xt cac acc ctg cag atc cac ctc ttt gaa aac cca gtt gag gtt ctt caa 931 His Thr Leu n Ile His Leu Phe G u Asn Pro Val G u Val Leu G n 265 270 275 aag gac ggc aag gtt gtt ggc ctg cgc acc gaa cgc acc tca ctt gat 979 Lys Asp G y Lys Val Val G y Leu Arg Thr G u Arg Thr Ser Leu Asp 280 285 290 ggc aac ggc ggc gta aac gga acc ggc gaa ttc aag gac tgg cca gtc 1027 Gy Asn Gy Gy Val Asn Gy Thr Gy Gu Phe Lys Asp Trp Pro Val 295 300 305 cag gct gtc tac cgc gca gtc ggc tac aag tcc gac ccc atc gac ggc 1075 G n Ala Val Tyr Arg Al a Val y Tyr Lys Ser Asp Pro lie Asp y 310 315 320 325 gtc cca ttc gat gag aac aag cac gtc atc cct aat gac ggc gga cat 1123 Val Pro Phe Asp G u Asn Lys His Val IIe Pro Asn Asp G y G y His 330 335 340 gtc ctc acc gct cca ggc gca gaa cca gta cca ggc ctc tat gca acc 1171 Val Leu Thr Ala Pro G y Ala G u Pro Val Pro y Leu Tyr Ala Thr 345 350 355 ggc tgg atc aag cgt gga cca atc ggt cta atc ggc aac acc aag tcc 1219 G y Trp lie Lys Arg G y Pro lie G y Leu Ile G y Asn Thr Lys Ser 360 365 370 gac gcc aag gaa acc acc gac atc ctc atc aag gat gcc gtc gcc ggt 1267 Asp Ala Lys u Thr Thr Asp Ile Leu Ile Lys Asp Ala Val Ala y 375 380 385 gta ctt gaa gct cca aag cac cag ggc gaa gaa gcc atc atc gag ctt 1315 Val Leu G u Ala Pro Lys Hi s n G y G u G u Al a Ie IIe G u Leu 390 395 400 405 ctc gat tcc cgc aac atc cca ttc acc acc tgg gaa ggc tgg tac aaa 1363 Leu Asp Ser Arg Asn Ile Pro Phe Thr Thr Trp G u Gy Trp Tyr Lys 410 415 420 ctc gac gca gca gag cgc gca ctc ggt gaa gcc gaa ggc cgc gag cgc 1411 Leu Asp Al a Al a G u Arg Al a Leu G y G u Ala G u G y Arg G u Arg 425 430 435 aag aag att gtt gat tgg gaa gaa atg gtc cgc cag gcc cgc gaa gct 1459 Lys Lys Ile Val Asp Trp G u G u Iet Val Arg G n Ala Arg G u Ala 440 445 450 cca gca att gtc taaattgttt taacgcgtga agc 1494 Pro Ala lie Val 455 <210> <211> 457 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> et Thr Thr Pro Leu Arg Val Ala Val II e G y Ala G y Pro Ala G y 1 5 10 Ile Tyr Ala Ser Asp Leu Leu lie Arg Asn G u G u Arg G u Val Phe 25 Val Asp Leu Phe G u G n Iet Pro Al a Pro Phe y Leu Ile Arg Tyr 40 Page 112 seqI i st car r ect ed. t xt Gy Val Ala Pro Asp His Pro Arg Ilie Lys Gy IlieVal 55 Lys Ser Leu Hi s li1e ValI G31y Tyr Ser 145 li1e As n Ph e As p 225 Ser Pr o ValI Ar g 305 As p As n G31y G31y As p 385 Al a ValI Lys5 Ph e O31u 115 G31y Al a Al a Tyr Ar g 1 95 Le u As p As p As p ValI 275 Ser Tr p I Ie G31y Tyr 355 Thr ValI I Ie As p I Ie Thr O31u Pr o I Ie Thr 1 65 Ser G31y Hi s As p ValI 245 Pr o O31n As p ValI G31y 325 Hi s Thr Ser G31y Le u 405 Pr o ValI Al a Ser Ph e 135 ValI As p Lys5 Al a Pr a 215 Al a O31n Thr As p As n 295 Al a Pr a Le u Tr p Al a 375 Le u As p Le u O31u Al a 1 G31y Ar g As n Le u As n 1 ValI I Ie O31u Le u O31n 265 Lys5 O31y Tyr As p Al a 345 Ly s O31u Al a Ar g Le u Ar g Ar g G31y Tr p G31y 1 ValI Al a Ph e ValI Al a 235 O31n Hi s ValI As n Al a 315 As n O31y O31y Thr Ly s 395 O31y Tyr Le u Ph e 125 Le u As p O31u O31u Pr a 205 ValI Ar g Al a Ph e Le u 285 Thr O31y Hi s Ou le 365 li1e O31n I Ie As p li1e O31y Al a Al a Ser 1 Hi s O31u Pr a Ser Ar g 255 As n Thr O31u Ly s I Ie 335 ValI Le u I Ie O31u Asn Ilie Pro Phe Thr Thr Trp Page 113 seql i st cor r ect ed. t xt Gu Gy Trp Tyr Lys Leu Asp Ala Ala G u Arg Ala Leu Gy u Ala 420 425 430 Su G y Arg u Arg Lys Lys Ile Val Asp Trp Gu Gu bet Val Arg 435 440 445 G n Ala Arg G u Ala Pro Ala Ile Val 450 455 <210> 81 <211> 786 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(763) <223> FRXA00075 <220> <221> VARIANT <222> 187 <223> Xaa Val or Ala <220> <221> VARIANT <222> 188 <223> Xaa Thr or Ser <220> <221> VARIANT <222> 209 <223> Xaa Lys or G n <220> <221> VARIANT <222> 212 <223> Xaa Arg <400> 81 tctaggagtg ttaaacagcc tggacttgaa acacctttaa ctacttgatt ttcacacc tgtttccata aaagggctca cgaaaggcaa cttcaaacac atg aca act ccc ctg ct cgc Ar g ct c Leu caa G n cac Hi s aag Lys gcc Al a at c SIle cct Pr o cgc Ar g cgc Ar g at c I e aat Asn ccg Pr o aag Lys cgc Ar g gga Gy gaa G u tt c Phe ggc Gy ct g Leu 75 gct Al a gag G u gga Gy at c SIle ct c Leu cct Pr o gaa G u at c SIle aag Lys aac Asn gct ggc Al a G y 15 gtg ttc Val Phe cgt tac Arg Tyr t cc ct g Ser Leu att gaa Ile G u 80 Page 114 IVet Thr Thr Pro Leu 1 att tac gca tcc gac I e Tyr Ala Ser Asp gtt gac ctt ttc gag Val Asp Leu Phe G u ggc gtt gct cca gac Sy Val Ala Pro Asp cac aac gtg ttg gac His Asn Val Leu Asp atc ggc aaa gac atc I e G y Lys Asp Ile 4- seqI i st car r ect ed. t xt acc gt c gaa gaa Thr Val G u G u ct c Le u cgc gac t ac t ac Arg Asp Tyr Tyr gca gt c gt g tt c Al a Val Val Phe t cc acc Ser Thr 1 00 ggc gca gtt 03 y Al a Val gac cgc gac ctc Asp Arg Asp Leu aac As n 110 at c ccc gga at t Ilie Pro 0 y Ilie gaa gca gaa O3 u Al a 03 u 115 ggc aac cca G31y As n Pro ggc tcct tc ggt gcc ggc gagt t cgt t ggc tt c tac gac G31y Ser Phe G31y Al a G31y 31u Phe Val G31y Phe Tyr Asp 120 125 130 cgc tt c Ar g Phe 135 gag cgc tcc tgg G31u Ar g Se r Tr p g at As p 140 ctg tct gca cag Leu Ser Al a G3 n gt c gct gt t at c Val Ala Val Ilie ggc G31y 1 gt t ggt aac gt c Val G31y Asn Val ct C gac gt a gcc Leu Asp Val Al a at c ct g gct aag Il e Leu Al a Lys ggc gac gag ct C G3 y Asp G3 u Leu aaa Ly s 1 70 gt c acc gaa at t Val Thr Gullie gac aac gt c t ac Asp Asn Val Tyr gac t cc Asp Ser 1 ctc aaa gaa Leu Lys G u ccc agc aca Pr o Ser Thr 200 aag gyc vvct gaa Lys Xaa Xaa G u cac gt t t tc gga Hi s Val Phe G y cgt cgg t gg Ar g Arg Trp 1 act cgr cca Thr Xaa Pro ggt caa gt t cac Gy Gn Val His aca gga act rraa Thr G3 y Thr Xaa ag a Ar g 210 ct c ccc cac cat caa cgt Leu Pro His His an Arg 215 <210> 82 <211> 221 <21 2> PRT ggt t gt a31yCy s 220 t gat ccagaa gacat cgact acg <21 3> Cor ynebact er i urn gl ut anm cumn <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> <220> <221 <222> <223> VARI ANT 1 87 Xa a V VARI ANT 1 88 I. or Al a Xaa Thr or Ser VARI ANT 209 Xaa Lys or a3 n VARI ANT 212 Xaa Ar g <400> 82 I'bt Thr Thr Pro Leu Arg 1 5 Val Ala Val Ilie 10 ay Ala a1y Pro Al a a3 y I I e Ty r Al a Se r Asp Leu Leu IlieArg Asn COu Ou Arg Ou Val Phe 25 Page 115 seql i st cor r ect ed. t xt Val Asp Leu Phe G u G n Met Pro Ala Pro Phe Gy Leu lie Arg Tyr 40 Gy Hi s I le Val Gy Tyr Ser 145 I le Asn Phe Xaa Al a Val Lys Phe Gu 115 Gy Al a Al a Tyr Ar g 195 Thr Asp Asp SIle Thr Gu Pr o l Ie Thr 165 Ser Tr p Pr o Pr o Pr o Val Al a Ser Phe 135 Val Asp Lys Ser Pr o 215 SIle Leu G u Al a 105 Gy Ar g Asn Leu Asn 185 Gy Hi s Gy Leu Ar g Ar g Gy Tr p Gy 155 Val Xaa Val Ar g I e Leu Asp Asp Gu Asp 140 Leu Thr Xaa Hi s Gy 220 Val Lys Sy Asn Tyr Tyr Leu Asn 110 Phe Val 125 Leu Ser Asp Val G u Ile G u Val 190 Pro Thr 205 Cys Ser SIe Asp I e Gy Al a Al a Ser 175 Hi s Gy <210> 83 <211> 672 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(649) <223> RXN00198 <400> 83 atgcaggatt agagcagcag caacggagcc agaatcagtc tcccaatttg atatccagcc ccgcgctcct ttccagcgcg ccgattccac tccatggccg atg tac ccc aac ctc 115 IVet Tyr Pro Asn Leu 1 ttc cgc acc gca acg gct cac gaa gaa ggc gaa tac atc atc act ggc 163 Phe Arg Thr Ala Thr Ala His Gu Gu Gy Gu Tyr lie lie Thr G y 15 gat gaa tca gcc gat gaa atc gca gcc ctg ggc ctc gcc gaa cgt gcc 211 Asp Gu Ser Ala Asp Gu Ile Ala Ala Leu y Leu Ala u Arg Ala 30 gca ggc tcc acg ctt ggt gaa cgt aaa ttt gct gtc aac acc gtg gaa 259 Ala y Ser Thr Leu G y G u Arg Lys Phe Ala Val Asn Thr Val G u 45 ttc cac ggc aac aac ggc cac gtc acc gga ctc acc ggc aac caa atc 307 Page 116 Phe cga Ar g ttc Phe caa G n cgc Ar g t ac Tyr at c SIle 150 ggt Sy Gy gt c Val gca Al a gga Gy ct c Leu 120 gca Al a gaa G u act Thr Asn ggc G y ct c Leu gca Al a gat Asp gac Asp cgc Ar g ct c Leu 170 Gy aaa Lys 75 gtt Val cac Hi s t cc Ser aac Asn gca Al a 155 cct Pr o Hi s cgt Ar g ctt Leu gaa G u gaa G u ggc Gy 140 tgc Cys gt a Val seql i st cor r ect ed. t xt Val Thr y Leu Thr y gaa cca atc gaa ggc acc G u Pro lie G u G y Thr 80 gtt gca ctt ggc ttc acc Val Ala Leu y Phe Thr 95 cta ggc gta ggt ttc gac Leu y Val y Phe Asp 110 tac cgc agc ccc acc aac Tyr Arg Ser Pro Thr Asn 125 130 cgt ggc cag tcc ctg atc Arg Gy n Ser Leu Ile 145 gcc gca gct atc gac gcc Ala Ala Ala lie Asp Al a 160 gca gtt gca cca cag gac Ala Val Ala Pro G n Asp Asn gaa G u ggc Gy gac Asp 115 t cc Ser gt g Val gat Asp gt g Val G n ttc Phe gca Al a 100 cgg Ar g cgc Ar g tgg Tr p ct c Leu ccg Pr o 180 SIe ccc Pr o gaa G u ggc Gy gtt Val gca Al a at g Met 165 ctg Leu gct gtc Al a Val tagcttgggt agaaaatgct aga <210> 84 <211> 183 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 84 IVet Tyr Pro Asn Leu Phe Arg Thr Ala 1 5 Tyr lie lie Thr y Asp u Ser Al a 25 Leu Ala G u Arg Ala Al a Gy Ser Thr 40 Val Asn Thr Val G u Phe Hi s y Asn 55 Thr y Asn n lie Arg Val Val Asn 70 Sy Thr G u Phe Pro Phe G u Ala Asp Phe Thr G y Al a G u n G y G y Leu 100 105 Phe Asp Asp Arg y Arg lie Leu Arg 115 120 Thr Asn Ser Arg Val Tyr lie Ala G y 130 135 Thr Al a Hi s 10 Asp G u Il e Leu G y G u Asn G y Hi s G y Lys Arg Leu Val Leu 90 Ala His G u Asp Ser G u Asp Asn G y 140 Page 117 G u Al a Ar g Val G u Val Leu Tyr 125 Ar g G u Al a Lys Thr Pr o Al a Gy 110 Ar g Gy Gy Leu Phe Gy I e Leu Val Ser G n seql i st cor r ect ed. t xt Leu I e Val Trp Ala Ile Ala Gu Gy Arg Ala Cys Ala Ala Ala lle 145 150 155 160 Asp Ala Asp Leu IVet G y G u Thr Ala Leu Pro Val Ala Val Ala Pro 165 170 175 G n Asp Val Pro Leu Ala Val 180 <210> <211> 305 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA00198 <400> ctc gtt ctt gtt gca ctt ggc ttc acc Leu Val Leu Val Ala Leu G y Phe Thr 1 5 gca cac gaa cta ggc gta ggt ttc gac Ala His G u Leu G y Val G y Phe Asp 25 gat tcc gaa tac cgc agc ccc acc aac Asp Ser G u Tyr Arg Ser Pro Thr Asn 40 gac aac ggc cgt ggc cag tcc ctg atc Asp Asn y Arg Gy On Ser Leu Ile 55 cgc gca tgc gcc gca gct atc gac gcc Arg Ala Cys Ala Ala Ala lie Asp Ala 70 ctc cct gta gca gtt gca cca cag gac Leu Pro Val Ala Val Al a Pro G n Asp tagcttgggt agaaaatgct aga <210> 86 <211> 94 <212> PRT <213> Cor ynebact er i um gl ut ai cum <400> 86 Leu Val Leu Val Ala Leu G y Phe Thr 1 5 Ala His G u Leu G y Val G y Phe Asp 25 Asp Ser G u Tyr Arg Ser Pro Thr Asn 40 Asp Asn y Arg Gy n Ser Leu Ile 55 Arg Ala Cys Ala Ala Ala lie Asp Ala gca Al a cgg Ar g cgc Ar g tgg Tr p ct c Leu 75 ccg Pr o gaa G u ggc Gy gtt Val gca Al a at g Met ct g Leu caa G n cgc Ar g t ac Tyr at c l Ie ggt Gy gct Al a ggc Gy at c I e at c SIle gcc Al a gaa G u gtc Val gga Gy ct c Leu gca Al a gaa G u act Thr ttg Leu cgc Ar g ggc Gy ggc Gy gca Al a Sy Ala u n y y Leu 10 Asp Arg Gy Arg lie Leu Arg Ser Arg Val Tyr Ile Ala y Val Trp Ala I e Ala u y Asp Leu NIet Gy u Thr Ala Page 118 seqi i st car r ect ed. t xt 70 Leu Pro Val Ala Val Ala Pro O31n Asp Val Pro Leu Ala Val <210O> 87 <21 1> 727 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (727) <223> RXN00365 <400> 87 gaagcct acg at cgct tgca cgcgaccaac aat t tccccg cccgcaccct gcgaaggcgc ct gcgt gct c ggt at caacg agt tcaccgg ccgt t tgt gc t ca Ser t gg Tr p gt c ValI cgc Ar g ggc G31y at c I Ie gt a ValI ct c Le u c ac Hi s aac As n 1 50 ggc G31y ac g Th r c aa O31n ggt G31y ggc G31y at g vb t cgc Ar g ac c Th r gca A a 120 ct c Le u at c I Ie aaa Ly s gag O31u gt a ValI ggc G31y ag c Ser t ac Ty r at c I Ie ccg Pr o G31y G31y g aa O31u gt c ValI 1 70 g aa O31u cca Pr o gct A a ac c Th r gt g ValI c aa O31n gcc A a cca Pr o cat Hi s 140 g ac As p at c I Ie gt c ValI at g vb t ct t Le u tt t Ph e g aa O31u g aa O31u g aa O31u 110 gcc A a gca A a g aa O31u G31y g aa O31u 15 t ct Ser gcc A a g aa O31u t ac Ty r gca A a 95 ct a Le u cgc Ar g at g vb t gt c ValI G31y 1 75 aaa Ly s ac c Th r gcc A a cgc Ar g aaa Ly s 80 gag O31u gcg A a g aa O31u g at As p t ct Ser 1 60 g ac As p at g vb t 1 gca A a ggg G31y gcg A a g ac As p at g G31y ct t Le u ct c Le u t ac Ty r 145 cca Pr o ac c Th r tt c Ph e ct g Le u c ag O31n g ac As p gag O31u ac a Th r tt c Ph e t ca Ser 130 ct c Le u at c G31y g aa O31u gt c ValI ct c Le u ct c Le u cgc Ar g tt c Ph e 1 00 gcg A a cca Pr a gcc A a gcc A a g ac As p 1 at t ct g t ca I I e Leu Ser cca Pr a ggc G31y gcc A a ac c Th r ggc G31y t gg Tr p c ag O31n at c G31y c aa O31n aaa Ly s 1 t gc Cys t tc ggc acc gca ct g cgc caa gga gca gaa t ca gt c acc caa t tt gat Page 119 seqi i st car r ect ed. t xt Phe O31y Thr Ala Leu Arg On Oy Ala Olu Ser Val Thr On Phe Asp 185 190 195 atc cgc ccc cgc gct cct ttc cag cgc gcc gat tcc I Ie Ar g Pro Ar g Ala Pro Phe O1n Ar g Ala Asp Ser 200 205 <210> 88 <211> 209 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 88 l'b t I Ie Al a Phe O3 y Leu Al a 03 n As p As p [vb t (1u O3 y Thr Leu Phe Leu Ser 130 Tyr Leu 145 Pr o I Ie Thr 03 y Leu Ser Ar g O31u Ser Val (3 n Leu Arg Leu Asn Arg Thr Phe 1 00 As p Al a 115 Val Pr o Thr Al a As n Al a Thr As p 1 8n Pr o O31y Al a Th r O31y Tr p O31n I Ie O31y O31n Ly s 1 65 Cys Ly s ValI ValI Al a 55 Le u As p O31y Le u As p 135 Ar g Ly s (31y Th r O31n O31y 40 O31y vb t Ar g Th r Al a 120 Le u I Ie Ly s Th r Ph e Pr o 25 Ser Hi s Ar g Ar g Ser 1 05 Th r As n As n ValI Al a 1 85 Le u I Ie Pr o ValI O31y O31u Ar g Th r I Ie O31y 1 li1e Ar g O31u Pr o Al a Th r ValI O31n Al a Pr a Hi s 140 As p I Ie O31n I Ie Ser O31y ValI Pr a vb t Al a ValI 125 Al a O31y O31y O31y ValI vb t Le u Ph e O31u O31u O31u 110 Al a Al a O31u O31y Al a 1 O31u Ser Al a O31u Ty r Al a Le u Ar g vb t ValI O31y 1 O31u Val Thr O31n 1 95 Ser Phe Asp I Ie Ar g Arg Ala Pro Phe On Arg Ala Asp 205 <210O> <211 <212> <213> <220> <221 <222> <223> <400> 89 727 DNA Cor ynebact er i urn gi ut ani curn ODS 101). (727) FRXA0 0365 89 Page 120 seqi i st car r ect ed. t xt gaagcct acg at cgct tgca cgcgaccaac aat t tccccg agt tcaccgg ccgt t tgt gc cccgcaccct gcgaaggcgc ct gcgt gct c ggt at caacg at g at t ct g t ca cca l'bt I Ie Leu Ser Pro t ca Ser t gg Tr p gt c ValI cgc Ar g ggc G31y at c I Ie gt a ValI ct c Le u c ac Hi s aac As n 1 50 ggc G31y Ph e at c I Ie ac g Th r c aa O31n ggt G31y ggc G31y at g vb t cgc Ar g ac c Th r gca A a 120 ct c Le u at c I Ie aaa Ly s ac c Th r ccc Pr a 200 gag O31u gt a ValI ggc G31y ag c Ser t ac Tyr at c I Ie ccg Pr a G31y G31y g aa O31u gt c ValI 1 70 ct g Le u gct A a g aa O31u cca Pr a gct A a ac c Th r gt g ValI c aa O31n gcc A a cca Pr a cat Hi s 140 g ac As p at c I Ie c aa O31n tt c Ph e at c I Ie t cc Ser ggc G31y 45 gt c ValI cca Pr a at g vb t gct A a gt g ValI 125 gcg A a ggt G31y ggt G31y gga G31y c ag O31n 205 gt c ValI at g vb t ct t Le u tt t Ph e g aa O31u g aa O31u g aa O31u 110 gcc A a gca A a g aa O31u ggc O31y gca A a 1 g aa O31u 15 t ct Ser gcc A a g aa O31u t ac Tyr gca A a 95 ct a Le u cgc Ar g at g vb t gt c ValI O31y 1 75 g aa O31u aaa Ly s ac c Th r gcc A a cgc Ar g aaa Ly s gag O31u gcg A a g aa O31u g at As p t ct Ser 1 g ac As p t ca Ser 1 gca A a ggg O31y gcg A a g ac As p at g vb t ggc O31y ct t Le u ct c Le u t ac Tyr 145 cca Pr a ac c Th r gt c ValI tt c Ph e ct g Le u c ag O31n g ac As p gag O31u ac a Th r tt c Ph e t ca Ser 130 ct c Le u at c O31y g aa O31u gt c ValI ct c Le u ct c Le u cgc Ar g tt c Ph e 1 00 gcg A a cca Pr a gcc A a gcc A a g ac As p 1 acc caa ttt Thr 0 n Phe cgc gcc gat t cc Ar g Ala Asp Se r <210O> <21 1> 209 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> l'b t I Ie Leu Ser Pro Ser Lys Thr Phe O31u Leu O31u I Ie Val O31u Lys 1 5 10 Page 121 seql i st cor r ect ed. t xt Ala Phe Arg u Gy Trp Val n Pro Val Ile Pro Ser Met Ser Thr Gy Al a Asp Met Gy Leu Leu Tyr 145 Pr o Thr Ser G n Ar g Asn Thr Asp 115 Val Thr Asn Thr Al a Thr Gy Tr p G n SIle Gy G n Lys 165 Cys Val Al a Leu Asp Sy Leu Asp 135 Ar g Lys Sy Ser Hi s Ar g Ar g Ser 105 Thr Asn Asn Val Al a 185 Pr o Val Sy G u Ar g Thr SIle Sy 155 I le Ar g Sy Val Pr o Met Al a Val 125 Al a Sy Sy Sy Leu Phe G u G u G u 110 Al a Al a G u Sy Al a 190 Al a G u Tyr Al a Leu Ar g Met Val Sy 175 G u Val Thr G n 195 Ser Phe Asp Ile Arg Arg Ala Pro Phe n Arg Ala Asp 205 <210> 91 <211> 480 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(457) <223> RXA00366 <400> 91 aaatcatgcc gcgcgatttc caaaaagtac tcaacatcat gccaagaccc agcaatcaag atcatggagg cagtgagcta gga ttc at c G y Phe lie ct g cgc ct c Leu Arg Leu cag at c gag Gn lie G u aaa tac tcc cga cgc gag Lys Tyr Ser Arg Arg G u atg gac cac tcc gag gtc IVet Asp His Ser G u Val 30 gaa cag gct gcc cgc tgc G u G n Al a Ala Arg Cys 45 cct gca Pro Al a 15 tac gaa Tyr G u atg gat Iet Asp Page 122 cgaaacggcc cacgctgagg atg gcc gac cca caa IVet Ala Asp Pro G n 1 cac cgc ccg gtc ccg His Arg Pro Val Pro aag gca ccg gca ggt Lys Ala Pro Ala y tgc ggt gtc ccg ttc Cys y Val Pro Phe seql i st cor r ect ed. t xt tgc cac gaa ggc tgc cca ctg ggc aac atc atc cct gag tgg aat gat 307 Cys His G u G y Cys Pro Leu G y Asn lie lie Pro G u Trp Asn Asp 60 ctg gta cgc caa ggt cgg tgg aag gaa gcc tac gat cgc ttg cac gcg 355 Leu Val Arg G n y Arg Trp Lys G u Ala Tyr Asp Arg Leu His Ala 75 80 acc aac aat ttc ccc gag ttc acc ggc cgt ttg tgc ccc gca ccc tgc 403 Thr Asn Asn Phe Pro G u Phe Thr y Arg Leu Cys Pro Ala Pro Cys 95 100 gaa ggc gcc tgc gtg ctc ggt atc aac gat gat tct gtc acc atc aaa 451 G u y Ala Cys Val Leu y Ile Asn Asp Asp Ser Val Thr Ile Lys 105 110 115 aac gtt tgagctggaa atcgtcgaaa aag 480 Asn Val <210> 92 <211> 119 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 92 IVet Al a Asp Pro n y Phe Ile Lys Tyr Ser Arg Arg u Pro Al a 1 5 10 His Arg Pro Val Pro Leu Arg Leu IVet Asp His Ser G u Val Tyr G u 25 Lys Al a Pro Al a G y n lle u u Gn Ala Ala Arg Cys IVet Asp 40 Cys G y Val Pro Phe Cys His G u G y Cys Pro Leu G y Asn lie lie 55 Pro G u Trp Asn Asp Leu Val Arg n y Arg Trp Lys G u Ala Tyr 70 75 Asp Arg Leu His Ala Thr Asn Asn Phe Pro G u Phe Thr G y Arg Leu 90 Cys Pro Ala Pro Cys G u y Ala Cys Val Leu y Ile Asn Asp Asp 100 105 110 Ser Val Thr Ile Lys Asn Val 115 <210> 93 <211> 1464 <212> DNA <213> Corynebacter i um gl ut arri cum <220> <221> CDS <222> (101)..(1441) <223> RXA02072 <400> 93 tacatggtgc cctcaatggg aaccaccaac atcactaaat ggcccaggta cacactttaa aatcgtgcgc gcatgcagcc gagatgggaa cgaggaaatc atg aca gtt gat gag 115 IVet Thr Val Asp G u Page 123 seql i st cor r ect ed. t xt cag G n cct Pr o gt c Val ct g Leu gac Asp tct Ser aac Asn t cc Ser gac Asp ttc Phe 150 gca Al a cac Hi s aag Lys t ac Tyr agc Ser 230 acc Thr ttc Phe tct Ser ttt Phe gaa G u gag G u ggc Gy ctt Leu ggc Gy acc Thr 120 aag Lys acc Thr aac Asn cgt Ar g ct g Leu 200 tgc Cys agc Ser gcg Al a gat Asp t at Tyr cag G n gac Asp gag G u gt c Val cca Pr o gt g Val ct g Leu aag Lys ct g Leu gga Sy 170 at g vbt tgg Tr p t ac Tyr cag G n gaa G u 250 agc Ser tac Tyr gca Al a cct Pr o cgt Ar g cac Hi s 75 tac Tyr aag Lys cca Pr o t cc Ser cac Hi s 155 gtt Val gct Al a ggt Gy tt c Phe aag Lys 235 aag Lys ggt Gy gac Asp gt g Val cat Hi s cag G n gt c Val aag Lys ttc Phe at c SIle gat Asp 140 cgc Ar g ggt G y aac Asn gga Sy gt g Val 220 at c SIle gct Al a tgg Tr p atg ctt ctg IVet Leu Leu 15 gca gag gtt Al a G u Val tac gct gat Tyr Al a Asp 45 ctc atc ttc Leu Ile Phe aac cgt ggt Asn Arg G y ggc ggc ctg G y G y Leu 95 ctg ggc ttt Leu G y Phe 110 ggt ggt ggc Sy y y 125 ct g gaa at c Leu G u Ile cac at c ggt Hi s I Ie G y ggc cac gag G y Hi s G u 175 cag cac gag G n Hi s G u 190 t cc ctg gt c Ser Leu Val 205 agt gaa at g Ser G u Mbet atc gtt tcc Ile Val Ser cag gaa ct c Sn G u Leu 255 gtt cat acc Val Hi s Thr aag Lys ttg Leu t ac Tyr cgt Ar g ttc Phe 80 cgc Ar g gag G u aag Lys at g vbt gag G u 160 at c SIle t cc Ser cgc Ar g at c SI e ggt Sy 240 ggc Sy 1 cgc Ar g gaa G u ggt Sy gt g Val cgc Ar g ttc Phe cag G n ggt G y cgt Ar g 145 t ac Tyr ggt G y ggc Sy acc Thr aag Lys 225 t cc Ser gca Al a aat Asn tct Ser ct c Leu cct Pr o gt g Val cac Hi s at c SIle gga G y 130 ttc Phe cgc Ar g t ac Tyr gtt Val gag G u 210 gct Al a ggc Sy acc Thr gct Al a ttg Leu at c SIle tgg Tr p cag G n cca Pr o ttt Phe 115 t cc Ser tgc Cys gac Asp ct g Leu ttg Leu 195 gca Al a aag Lys aac Asn gtt Val ggc G y aag Lys cag G n gtt Val ttc Phe tct Ser 100 aaa Lys gac Asp cag G n gtt Val ttt Phe 180 acc Thr act Thr ggc Sy gt a Val at t SIle 260 gag G u at c SIe cgc Ar g gat Asp aac Asn gta Val aac Asn ttc Phe t cc Ser cct Pr o 165 ggc G y ggt Sy ggc Sy gag G u gca Al a 245 ggt Sy cct aac ggc gtt gac gtg Pro Asn y Val Asp Val Page 124 seql i st cor r ect ed. t xt 270 gct aag ctc cgc gaa atc aag gaa gtt cgt Ala Lys Leu Arg G u Ile Lys G u Val Arg 280 285 tac gcc gac gaa gtt gaa ggc gca acc tac Tyr Ala Asp G u Val G u Gy Ala Thr Tyr 295 300 tgg gat ctc aag tgc gat atc gct ctt cct Trp Asp Leu Lys Cys Asp lie Ala Leu Pro 310 315 ctc aac ggc gag aac gct aag act ctt gca Leu Asn Gy G u Asn Al a Lys Thr Leu Ala 330 335 gtt gct gaa ggc gcg aac atg cct tcc acc Val Ala G u Gy Ala Asn bet Pro Ser Thr 345 350 ttc cgt gag cgc gac atc cgc ttc gga cca Phe Arg G u Arg Asp Ile Arg Phe y Pro 360 365 ggt ggc gtt gca acc tcc gct ctg gag atg Gy y Val Ala Thr Ser Ala Leu u IVet 375 380 gat tcc tgg agc ttc gag tac acc gac gag Asp Ser Trp Ser Phe G u Tyr Thr Asp G u 390 395 aag aac atc ttc aag acc tgt gca gag acc Lys Asn Ile Phe Lys Thr Cys Ala G u Thr 410 415 gag aac gat tac gtt gtc ggc gct aac att G u Asn Asp Tyr Val Val Gy Ala Asn Ile 425 430 gct gac gcg atg ctg gca cag ggc gtc atc Ala Asp Ala IVet Leu Ala n y Val lie 440 445 t aa <210> 94 <211> 447 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 94 NIet Thr Val Asp G u G n Val Ser Asn Tyr 1 5 10 Arg Asn Ala G y G u Pro u Phe His n 25 Su Ser Leu Lys lie Val Leu G u Lys Asp 40 Sy Leu lie n Arg Leu Cys u Pro u 55 Val Pro Trp Val Asp Asp G n y G n Val Pag cgc Ar g cac Hi s tgt Cys 320 gac Asp cct Pr o ggc Sy cag G n cgc Ar g 400 gca Al a gct Al a gca Al a acc Thr 305 gca Al a aac Asn gag G u aag Lys cag G n 385 ct c Leu gca Al a ggc G y cgc gta Arg Val 290 gac ggt Asp y act cag Thr G n ggc tgc Sy Cys gct gtt Al a Val 355 gca gct Al a Al a 370 aac gct Asn Ala cag gtg G n Val gag tat G u Tyr ttc aag Phe Lys 435 t cc Ser t cc Ser aac Asn cgt Ar g 340 gag G u aac Asn tcg Ser at c SIe gga G y 420 aag Lys 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1461 1464 taagacccct gcgctttact Tyr Asp IVet Leu Leu Lys Ala Val Ala G u Val Leu Pro His Tyr Ala Asp Tyr Arg G n Leu Ile Phe Arg Hi s Val Asn Arg G y Phe e 125 seqI i st car r ect ed. t xt Ar g Ph e O31n G31y Ar g 145 Tyr G31y G31y Thr Ly s 225 Ser Al a As n Al a Thr 305 Al a As n O31u Ly s O31n 385 Le u Al a O31n Pr o Ph e 115 Ser CYS As p Le u Le u 1 95 Al a Ly s As n ValI ValI 275 ValI G31y O31n cys ValI 355 Al a Al a ValI Tyr As n ValI As n Ph e Ser Pr o 1 65 O31y O31y O31y O31u Al a 245 O31y ValI ValI I Ie O31u 325 Ph e ValI Al a Ar g vb t 405 Hi s Ser Al a Leu As n Leu 03 y Ser Leu Thr 120 Asp Pro Lys 135 Phe I'b t Thr 150 Al a 03 y Asn Hi s Tyr Ar g Lys 03 y Leu 200 Tyr O31y Cys 215 Ser Ilie Ser 230 Thr Tyr Al a Phe Ser Asp Al a Lys Leu 280 Tyr Al a Asp 295 Trp Asp Leu 310 Leu Asn 03 y Val Al a O31u Phe Ar g O31u 360 O3 y 03 y Val 375 Asp Ser Trp 390 Lys Asn Il e 31u Asn As i O31y Pro Tyr 90 li1e Val Lys 1 05 O31y Leu Pr o O3 y Lys Ser O3 u Leu Hi s 1 1I e 03 y Val 1 70 Ar g Nib t Al a 1 85 Thr Tr p O31y Val Ty r Phe O3 y 03 n Lys 235 1I e 03 u Lys 250 Ser Ser 03 y 265 Ar g O31u I Ie 31u Val O31u Lys Cys As p 315 O3 u As n Al a 330 O3 y Al a As n 345 Ar g As p I Ie Al a Thr Ser Ser Phe 03 u 395 Phe Lys Thr 410 Tyr Val Val 425 Lys 03 y 03 y Phe Leu 03 y 110 Ilie O31y O31y 125 Asp Leu 03 u 140 Ar g Hi s Ilie O3 y 03 y Hi s Asn 03 n Hi s 1 O31y Ser Leu 205 Val Ser O31u 220 Ile IlIe Val Al a 03 n 03 u Tr p Val Hi s 270 Lys 03 u Val 285 O3 y Al a Thr 300 Il e Al a Leu Lys Thr Leu I'b t Pr o Ser 350 Ar g Phe (31y 365 Al a Leu 03 u 380 Tyr Thr Asp Cys Al a 03 u Le u Ph e Oy le Oy Ou 1 O31u ValI vb t Ser Le u 255 Th r Ar g Ty r Pr o Al a 335 Th r Pr o vb t O31u Th r 415 O y Al aAsn Il e Al a 430 Page 126 seqi i st car r ect ed. t xt G31y Phe Lys Lys Val Ala Asp Ala l'bt Leu Ala O31n 31y Val I Ie 435 440 445 <210O> <21 1> 1461 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1438) <223> RXA00323 <400> cgcgacat cc t t gagt aact ct gagaaaaa ct acccccga at gcgcagt c gat gt cccat cgct gcgt ag at tagtt t tc t gggagt at a aaagt ggcaa g aa O31u ct a Le u cct Pr o t ca Ser cgc Ar g t ca Ser gga O31y c aa O31n gag O31u ccc Pr o 1 50 ccg Pr o cct Pr o gt a ValI tt c Ph e g aa O31u at t I Ie g at As p ct g Le u cca Pr a gct A a 120 t at Ty r g ac As p tt c Ph e gag (31u ag c Ser g ac As p gag O31u O31y t cg Ser gca A a tt t Ph e g at As p gt g ValI ggc O31y cga Ar g 1 70 t cc Ser gcc A a at t I Ie t ct Ser t ac Ty r aca Th r 75 gca A a t ct Ser gaa O31u caa O31n gga G31y 155 aac As n cac Hi s at t I Ie ct t Le u gcg A a gcg A a tt c Ph e cgc Ar g g ac As p ggc G31y ag c Ser 140 t at Ty r gcg A a cat Hi s g aa (31u G31y tt g Le u 45 cgt Ar g c ag O31n ct g Le u ccg Pr a tt g Le u 125 ct t Le u tt c Ph e at g vb t g aa (31u g aa O31u c ac Hi s g aa O31u at c I Ie gt c ValI tt t Ph e cgc Ar g 110 ac c Th r cgc Ar g g ac As p gt a ValI act Th r 1 90 cgc Ar g 15 tt g Le u g aa O31u t cg Ser ct c Le u t gc cys 95 c aa O31n t gc cys ac c Th r c aa O31n gcg A a 1 75 gca A a g ac As p aag Ly s ggc O31y g aa O31u cca Pr a 80 g at As p gt g ValI at g vb t aac As n gcc A a 1 60 ct g Le u cct Pr a at g vb t 1 at t I Ie t ca Ser at c I Ie gcg A a 65 ct a Le u gt c ValI ct g Le u at c I Ie gga O31y 145 ac a Th r gag O31u (31y aag Ly s gt g ValI gga O31y 50 g ac As p g aa O31u ac g Th r cgc Ar g t ca Ser 130 ct g Le u tt c Ph e g aa O31u c ag (31n aac agc gaa cag Asn Ser 0 u 0 n tt t Ph e gt t ValI 35 tt c Ph e ac c Thr gcg A a at g vb t ag g Ar g 115 cca Pr a cca Pr a aat As n ct c Le u c aa O31n 1 gt g ValI 20 gt g ValI g at As p at t O31y cca Pr a 1 00 c ag O31n gag O31u cct Pr a gag O31u ggc O31y 1 g aa (31u cgt Ar g gct A a ggc O31y gcc A a at c lIe g ac As p gt c ValI at t I Ie gt g ValI gcg A a 1 at c I Ie at c I Ie Page 127 seql i st cor r ect ed. t xt gat Asp ttc Phe t ca Ser 230 acg Thr gac Asp at c SIle aat Asn acc Thr 310 cgt Ar g acc Thr ttg Leu gat Asp gat Asp 390 gag G u cgc Ar g tta cgc cat Leu Arg Hi s 200 cgc t ac at c Arg Tyr II e 215 ttt atg ccc Phe Met Pro cac at g t cc His Met Ser gat tct tac Asp Ser Tyr 265 ttg cat cac Leu Hi s Hi s 280 tcc tac aaa Ser Tyr Lys 295 tgg ggt gta Trp y Val ttg aat aag Leu Asn Lys gct t gt aac Ala Cys Asn 345 aaa ggc at t Lys G y Ile 360 at c t cc aac Ile Ser Asn 375 ct g cca agc Leu Pro Ser ctt gtt gct Leu Val Al a aat aag tgg Asn Lys Trp 425 gcg Al a at g Mat aag Lys tta Leu 250 at g Met gct Al a cgc Ar g tct Ser gag G u 330 cca Pr o aaa Lys ttg Leu agc Ser gac Asp 410 cgt Ar g gat Asp aaa Lys cca Pr o 235 ttt Phe ct g Leu cca Pr o at c SIle aat Asn 315 gag G u t at Ty r gaa G u agc Ser ctt Leu 395 at c SIle gaa G u gcg Al a cag G n 220 ttc Phe gag G u t cc Ser gaa G u gt g Val 300 cgt Ar g tcg Ser ttg Leu ggt Gy ttc Phe 380 gat Asp ct c Leu tgg Tr p ctc acc atg gcc gac Leu Thr IVet Ala Asp gca Al a gaa G u gat Asp acc Thr 270 acc Thr gga Gy gcg Al a cgg Ar g ttt Phe 350 gag G u gaa G u gca Al a gag G u gac Asp 430 agg Ar g cat Hi s acc Thr 255 gca Al a gct Al a aac Asn ct g Leu gt g Val 335 t ca Ser ct c Leu cgt Ar g ct g Leu cac Hi s 415 t ac Tyr gac Asp gca Al a 240 aac Asn aaa Lys gt g Val gaa G u gtt Val 320 gag G u gt g Val gac Asp cgc Ar g cgc Ar g 400 gtt Val caa G n caa G n 225 ggc G y gcg Al a cag G n acc Thr gct Al a 305 cgt Ar g gt g Val at g Met gag G u gcc Al a 385 caa G n ttt Phe gag G u aac at c at g Asn lI e Iet 210 ggc gtc ggg G y Val G y t cc gcc at g Ser Al a IVet ttc cac gat Phe Hi s Asp 260 ttc atc gct Phe IIe Al a 275 aac cag tgg Asn n Trp 290 cca act gcg Pro Thr Al a gtt cct acc Val Pro Thr cgt ctt cct Arg Leu Pro 340 ct c ggc gct Leu G y Al a 355 cca gct gag Pr o Al a G u 370 atg ggc t ac IVet G y Tyr at gaa aag Iet G u Lys gag ttt ttc G u Phe Phe 420 cag at c act G n Ile Thr 435 acc Thr gca Al a cac Hi s 245 cca Pr o gga G y gt c Val gca Al a t ac Tyr 325 gat Asp ggt Gy gac Asp aac Asn t ca Ser 405 ttg Leu ccg Pr o 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1458 1461 tgg gag ct c Tr p G u Leu 440 gaa cga aac aat ctt Arg Asn Asn Leu gat tac Asp Tyr 445 tagacttttg cactccaatg Page 128 seqi i st car r ect ed. t xt <210O> 96 <21 1> 446 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 96 l'bt Asn Ser 31u O31n O31u Phe Val Leu li Se le A a Le u ValI Le u I Ie O31y 145 Thr O31u O31y As p O31n 225 O31y A a O31n Thr A a 305 Ar g Ph e ValI 35 Ph e Thr A a vb t Ar g 115 Pr a Pr a As n Le u O31n 1 95 li1e ValI A a Hi s I Ie 275 O31n Thr Pr a Ar g A a O31y A a I Ie As p ValI I Ie ValI A a 1 65 le le Thr A a Hi s 245 Pr a O31y ValI A a Tyr 325 Tr p A a A a Pr a Ly s O31n Le u Ph e 135 Thr As n ValI Le u Ar g 215 Ph e Hi s As p Le u Ser 295 Tr p Le u Thr 25 Le u O31u Pr a O31n Ser 1 05 A a Le u As n Ar g Ph e 1 85 Hi s I Ie Pr a Ser Tyr 265 Hi s Ly s ValI Ly s Ser 10 As p O31u O31y Ser A a 90 Ph e As p ValI O31y Ar g 1 70 Ser A a vb t Ly s Le u 250 vb t A a Ar g Ser A a I Ie Ser Tyr Thr 75 A a Ser O31u O31n O31y 1 55 As n Hi s As p Ly s Pr a 235 Ph e Le u Pr a I Ie As n 315 I Ie Le u A a A a Ph e Ar g As p O31y Ser 140 Tyr A a Hi s A a O31n 220 Ph e O31u Ser O31u ValI 300 Ar g O31u O31y Le u Ar g O31n Le u Pr a Le u 125 Le u Ph e vb t O31u Le u 205 ValI O31n O31y Ly s Ph e 285 Tyr Ser O31u Hi s O31u I Ie ValI Ph e Ar g 110 Thr Ar g As p ValI Thr 1 Thr A a O31u As p Thr 270 Thr O31y A a Ar g Asp Leu Lys O31u (1y Ser 03 u Leu Pro Cys As p O3 n Val Cys l'b t Thr As n O3 n A a 1 A a Leu 1 Ala Pr o [vb t A a Ar g As p Hi s A a 240 Thr As n 255 Al a Lys Al a Val As n (1u Leu Val 320 Val G31u 335 (3u (3u Se r Ar g Ar g 330 Page 129 ValI vb t (31u A a 385 O31n Ph e (31u Le u G31y 355 A a G31y G31u Ph e I Ie 435 As p G31y As p As n Ser 405 Le u Pr o A a Ly s I Ie 375 Le u Le u As n (31u seql i st car r ect ed. t xt Cys Asn Pro Tyr Leu Ala 345 O3 y I I e Lys 03 u 03 y Ty r 360 365 Ser Asn Leu Ser Phe Ar g 380 Pr o Ser Ser Leu As p O31n 395 Val Ala As p I Ie Leu G31y 410 Lys Tr p Arg O31u Trp Ar g 425 Leu Ar g As n As n Leu As p 440 445 Ph e 350 O31u O31u A a O31u As p 430 Ty r Ser Le u Ar g Le u Hi s 415 Ty r <210O> 97 <21 1> 1554 <21 2> DNA <21 3> Cor ynebact er i urn gI ut anri cumn <220> <221 CDS <222> (1531) <223> RXA00335 <400> 97 act acat t tg cagccaagt c t act act tga t ct tcaaagg t acaaat aaa ccgt tccgcc cat gt caat g aggagt cacc tcagcaattg tgaacaaagc gcg t tt gaa A a Phe 03 u ccg Pr o g ac As p cca Pr o g ac As p aat As n gca A a gag (31u g aa O31u cga Ar g gcc A a t cc Ser ct g Le u ac c Thr tt c Ph e gt c ValI ac c Thr tt c Ph e at c I Ie g ac As p aac As n cgc Ar g tt c Ph e ct t Le u gca A a ggC G31y gga G31y aag Ly s cca Pr o aag Ly s ggC G31y ac a Thr ac c Thr gcc A a tt c Ph e aac As n 110 g aa O31u gag O31u g aa O31u at c I Ie ct t Le u c ac Hi s gca A a gt c ValI c ac Hi s ggt G31y g aa O31u cca Pr a cct Pr a aag Ly s gag O31u tt c Ph e ct c Le u t ct Ser tt c Ph e tt c Ph e gca A a 115 tt c Ph e ag c Ser gca A a g ac As p cgc Ar g ac c Th r 1 00 gag (31u Page 130 tac ctg gca Tyr Leu Al a 120 tcc acc ggc att Ser Thr G y lie gc Al 12 seql i st cor r ect ed. t xt a gac acc tgc aac tt a Asp Thr Cys Asn Ph c e 0 ggc gcc gag G y Al a u 5 13 gct gag Al a G u 135 ttc tac ctc ttc Phe Tyr Leu Phe gac tcc gt t Asp Ser Val 140 cgc t ac t cc Arg Tyr Ser 145 acc gag atg aac Thr G u ebt Asn ggc ttc tac gaa G y Phe Tyr G u gta gat acc gaa gaa ggc tgg tgg aac cgt ggc Val Asp Thr u u G y Trp Trp Asn Arg y 155 160 165 aag gaa acc aac Lys G u Thr Asn ct c Leu 170 gac ggc acc cca Asp y Thr Pro ctg ggc gca aag Leu G y Al a Lys aac cgc Asn Arg 180 gtc aag ggt Val Lys G y gtg cgc gat Val Arg Asp 200 tac ttc cca gta Tyr Phe Pro Val cca tac gac caa Pro Tyr Asp G n acc gtt gac Thr Val Asp 195 ttc gct ctt Phe Al a Leu gac atg gtt cgc Asp Ibet Val Arg ctc gca gct tcc Leu Al a Al a Ser ggc 10y 210 gag cgt G u Ar g 215 ttc cac cac gaa Phe His His G u gtc ggt ggc gga cag cag gaa atc aac tac Val Gy Gy Gy Gn Gn Gu Ile Asn Tyr 220 225 cgc Ar g 230 ttc aac acc atg Phe Asn Thr IVet cac gcg gca gat Hi s Al a Al a Asp gat Asp 240 atc cag acc ttc Ile n Thr Phe tac atc atc aag aac acc gct cgc ctc cac Tyr lie lie Lys Asn Thr Ala Arg Leu His 250 255 ggc aag gct gca G y Lys Al a Al a acc ttc Thr Phe 260 atg cct aag IVet Pro Lys ctg gct ggc gac Leu Al a G y Asp aac Asn 270 ggt tcc ggc atg G y Ser G y et cac gct cac Hi s Al a Hi s 275 cag tcc ctc tgg aag gac ggc aag cca ctc ttc cac gat gag tcc ggc G n Ser Leu Trp Lys Asp G y Lys Pro Leu Phe His Asp G u Ser G y 280 285 290 t ac gca Tyr Al a 295 ggc ctg tcc gac G y Leu Ser Asp at c Ile 300 gcc cgc tac tac Ala Arg Tyr Tyr ggc ggc atc ctg G y G y Ile Leu cac Hi s 310 cac gca ggc gct Hi s Al a G y Al a ctg gcg ttc acc Leu Ala Phe Thr aac Asn 320 gca acc ctg aac Ala Thr Leu Asn tac cac cgt ctg Tyr His Arg Leu gtt Val 330 cca ggc ttc gag Pro G y Phe G u cca atc aac ct g Pro Ile Asn Leu gtg tac Val Tyr 340 1027 1075 1123 1171 1219 1267 t ca cag cgc Ser G n Arg aac ccg aag Asn Pro Lys 360 cgt tcc gct gct Arg Ser Ala Ala gt c Val 350 cgt atc cca atc Arg lie Pro lie acc gga t cc Thr G y Ser 355 cca tca ggc Pro Ser G y gca aag cgc atc Ala Lys Arg lie gaa 35u 365 ttc cgc gct cca Phe Arg Ala Pro gac Asp 370 aac cca Asn Pro 375 tac ctg ggc ttt Tyr Leu G y Phe gca Al a 380 gcg atg atg atg Al a IVet IVet IVet gcc Al a 385 ggc ctc gac ggc G y Leu Asp G y Page 131 atc aag aac cgc atc gag cca Ile Lys Asn Arg lie Gu Pro 390 395 gaa cta cca cca gag gaa gct Su Leu Pro Pro Gu Gu Al a 410 ctg gaa gca tcc ctg aag gca Leu G u Ala Ser Leu Lys Al a 425 gag tct gac gtc ttc acc gag Su Ser Asp Val Phe Thr G u 440 aag tac gac aac gag atc tcc Lys Tyr Asp Asn G u IIe Ser 455 460 gaa ttc gaa ttg tac ttc gac G u Phe G u Leu Tyr Phe Asp 470 475 <210> 98 <211> 477 <212> PRT <213> Cor ynebact er i um gl ut ar <400> 98 Val Ala Phe G u Thr Pro G u 1 5 Asn Val G u Phe Val Asp Val G n His Phe Ser Ile Pro Ala G u G y Leu Ala Phe Asp G y 55 Asp G u Ser Asp IVet Asn Leu 70 Asp Pro Phe Arg Lys Ala Lys Asp Pro Phe Thr Arg G u Ala 100 Arg Lys Ala G u G n Tyr Leu 115 Asn Phe G y Al a u Al a G u 130 135 Ser Thr G u IVet Asn Ser G y 145 150 Trp Trp Asn Arg y Lys G u 165 Sy Ala Lys Asn Arg Val Lys 180 seql i st cor r ect ed. t xt cac gct cca gtg gac aag gac ctc His Ala Pro Val Asp Lys Asp Leu 400 gca tcc att cca cag gca cca acc Ala Ser Ile Pro G n Ala Pro Thr 415 420 ctg cag gaa gac acc gac ttc ctc Leu G n G u Asp Thr Asp Phe Leu 430 435 gat ctc atc gag gcg tac atc cag Asp Leu Ile u Ala Tyr Ile n 445 450 cca gtt cgc ctg cgc cca acc ccg Pro Val Arg Leu Arg Pro Thr Pro 465 tgc taattcactt agctagccga tag Cys 1315 1363 1411 1459 1507 1554 SIle Phe 25 Ser Ser Pr o Leu Ser 105 Ser Tyr Tyr Asn Sy 185 Lys Asp Asp Ar g Leu Val Asp Sy Phe Val 155 Asp Phe Phe Leu Al a Sy Sy Lys Pr o SIle Asp 140 Asp G y Pr o SIle Pr o Asp Phe Thr Phe Ar g Al a 125 Ser Thr Thr Val Lys Sy Thr Thr Al a Phe Asn 110 Asp Val G u Pr o Al a 190 Asp Thr SIe Thr Thr Val Val Thr Ar g G u Asn 175 Pr o Asp G n Thr Val Asp Val Arg Asp Asp IVet Val Arg Asn Leu Ala Ala Page 132 seqi i st car r ect ed. t xt 205 (31y (1y (1y (1n Ser (31n 225 li1e Ly s G31y Hi s I Ie 305 A a I Ie Pr o Pr o A a 385 As p O31n Thr A a Ar g 465 Ph e I Ie Thr A a Hi s 275 O31u G31y Le u Le u Thr 355 Pr o Le u As p Pr o Ph e 435 li1e Thr Le u Tyr Ly s 245 Ph e Hi s G31y Le u Ser 325 Tyr Ser G31y G31y Tyr 405 Ser Thr Tyr O31n Ar g 215 Ph e I Ie Pr a Ser A a 295 Hi s Hi s O31n Pr a Pr a 375 Ly s Le u (31u Ser Tyr 455 Ph e Ph e Hi s Hi s 03 u As n I Ie Ly s Le u 280 G31y A a Ar g Ar g Ly s 360 Tyr As n Pr a A a As p 440 As p (31u Thr Ly s Pr a 265 Tr p Le u G31y Le u As n 345 A a Le u Ar g Pr a Ser 425 ValI As n Le u Le u 235 Thr A a As p As p ValI 315 Pr a Ser Ar g Ph e (31u 395 (31u Ly s Thr I Ie Ph e 475 ValI 220 Hi s A a G31y Gy le 300 Le u G31y A a I Ie A a 380 Pr a A a A a (31u Ser 460 As p A a Ar g As p Ly s 285 A a A a Ph e A a (31u 365 A a Hi s A a Le u As p 445 Pr a Cys A a Le u As n 270 Pr a Ar g Ph e (31u ValI 350 Ph e vb t A a Ser O31n 430 Le u ValI As p Hi s 255 O31y Le u Tyr Thr A a 335 Ar g Ar g vb t Pr a I Ie 415 O31u I Ie Ar g <210O> 99 <21 1> 3258 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (3235) <223> RXA00324 <400> 99 t ccgt gggag ct ccgaaaca at ct t gat t a ct agact t t t gcact ccaat ggaaaccct a cggcgaccca attgcgaccc gatgaaggag gggagaagct atg tca gga ccg tta 115 Page 133 seql i st cor r ect ed. t xt bet Ser G y Pro Leu aga Ar g gtt Val cat Hi s t ca Ser t at Tyr caa G n ttg Leu cag G n cag G n gag G u 150 cct Pr o ttg Leu tta Leu gtt Val gct Al a 230 aag Lys gaa G u tct Ser ggt Gy gca Al a gca Al a at t SIle ggt Sy aaa Lys 120 ct g Leu ttc Phe ttt Phe tgg Tr p ggt Gy 200 ttt Phe act Thr gtt Val aaa Lys tcg Ser cgc Ar g gat Asp gaa G u cag G n tcg Ser tta Leu gaa G u gat Asp acg Thr 170 t at Tyr t at Tyr aca Thr gct Al a t ca Ser 250 gt c Val ct g Leu aat Asn ccc Pr o gca Al a 75 gat Asp gct Al a aaa Lys tct Ser gca Al a 155 gct Al a cgc Ar g ccc Pr o gt g Val tta Leu 235 gct Al a gtt Val aaa Lys gtt Val gat Asp at c l Ie gaa G u gt c Val ctt Leu gt g Val 140 cac Hi s agt Ser acg Thr acc Thr acc Thr 220 gct Al a tta Leu ggc G y tct Ser gag G u 45 gt c Val ggc G y aaa Lys ggt G y gat Asp 125 aaa Lys aac Asn gtt Val ttg Leu gac Asp 205 at g lbt gt g Val tct Ser ttt Phe gag G u tct Ser gcg Al a gag G u ct a Leu gat Asp 110 gcg Al a gct Al a at t SIle acc Thr ct g Leu 190 at g Met cag G n gca Al a gt c Val aga Ar g gcc Al a gat Asp aac Asn gct Al a gt c Val ttg Leu tcg Ser cct Pr o cga Ar g 160 cct Pr o cgg Ar g aga Ar g agc Ser gcc Al a 240 gcg Al a gac Asp caa G n ttg Leu ctt Leu cga Ar g cgc Ar g gt c Val agg Ar g gct Al a 145 gac Asp gaa G u at t SIle aaa Lys gac Asp 225 aat Asn at g let cca Pr o gca Al a ttg Leu ctt Leu aac Asn ctt Leu gcc Al a gaa G u 130 gt g Val gat Asp gca Al a gct Al a ggt G y 210 ct a Leu gtt Val ggc G y ct g Leu gat Asp tgg Tr p at t SIle gag G u ttt Phe aat Asn 115 gag G u ctt Leu ttg Leu gag G u gcg Al a 195 ggc G y gct Al a t at Tyr aaa Lys cca Pr o ct a Leu ggc G y cgg Ar g ctt Leu gca Al a 100 cct Pr o at g let gag G u agc Ser cga Ar g 180 cat Hi s gat Asp gat Asp ggt G y tgt Cys 260 gcg cag gaa ttg aac tac att tca gat gtg gac gtg gtg ttt gtt gca Page 134 Ala On u Leu Asn Tyr Ile Se 265 seql i st cor r ect ed. t xt r Asp Val Asp Val Val 270 gag G u at c SIle ggt G y 310 t ac Tyr cgt Ar g ctt Leu gat Asp ttg Leu 390 gag G u acg Thr cag G n gag G u cgc Ar g 470 ttg Leu aaa Lys ttg Leu gca Al a 280 agc Ser agt Ser cgc Ar g at g Met ccg Pr o 360 caa G n gat Asp gct Al a cgg Ar g t at Tyr 440 ttg Leu cac Hi s cgc Ar g at g Met agt Ser 520 tct Ser tcg Ser gct Al a gcg Al a 330 ggt Gy at t SIle at g Met gag Su cag G n 410 cgg Ar g ggt Sy ct g Leu tta Leu tct Ser 490 cgg Ar g ct g Leu aaa Lys tt c Phe ctt Leu 315 gaa G u gat Asp tgg Tr p cgc Ar g ct g Leu 395 ct c Leu t ca Ser cgt Ar g tt g Leu ccg Pr o 475 ggg G y cat Hi s ttt Phe t ca Ser ttt Phe 300 gt g Val acc Thr at t SIle acg Thr cgt Ar g 380 aag Lys ctt Leu acg Thr gaa G u gag G u 460 aaa Lys ttt Phe ttg Leu t at Tyr aca Thr 285 gag G u cgc Ar g tgg Tr p aac Asn gct Al a 365 cga Ar g ctt Leu cag G n gt a Val gac Asp 445 cat Hi s cct Pr o act Thr cgt Ar g cgg Ar g 525 cgc Ar g gt g Val tct Ser gag G u ctt Leu 350 agc Ser gt g Val ggt G y at g Met aat Asn 430 ggg G y cgc Ar g gat Asp ggt G y aag Lys 510 cca Pr o gca Al a gca Al a gat Asp 320 cag G n cag G n cgg Ar g gac Asp ggt G y 400 cat Hi s ttg Leu aat Asn caa G n cga Ar g 480 at g Met cgt Ar g ct g Leu gca Al a gca Al a 305 t cc Ser gca Al a t cc Ser gaa G u aat Asn 385 ggt G y ggt G y cat Hi s ct c Leu ttg Leu 465 at g Met gag G u ttg Leu aac Asn gag G u 290 ctt Leu cat Hi s ct g Leu t at Tyr t ca Ser 370 gtt Val ttg Leu cgc Ar g gt g Val at t SIle 450 gag G u aat Asn caa G n cag G n tct Ser 530 Phe Val Al a 275 ctc att cgc Leu II e Ar g cgc cca gaa Arg Pro G u atg gcg tat IVet Al a Tyr 325 ct g aaa gct Leu Lys Al a 340 gtg gat gct Val Asp Al a 355 ttt gtc aca Phe Val Thr ccg gaa gac Pro G u Asp agg gat gtg Arg Asp Val 405 att gat gag Ile Asp G u 420 ttg gtt gat Leu Val Asp 435 gag t cg t at G u Ser Tyr cgg atc aag Arg Ile Lys atg cgc tgg IVet Arg Trp 485 agt tcg gcc Ser Ser Al a 500 att cag tcg Ile G n Ser 515 gtg gtc aac Val Val Asn 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 ttg agc gcg gat gcc atc agg ttg tct ccg gat gct gca aag cta caa Page 135 s Leu Ser Ala Asp Ala l e Arg Leu 535 540 eql i st cor r ect ed. t xt Ser Pro Asp Ala Al a Lys Leu G n ttg Leu 550 act Thr ttg Leu gcg Al a agc Ser ttg Leu 630 t cc Ser aaa Lys acg Thr tcg Ser aac Asn 710 gat Asp aac Asn ggc G y gat Asp gcc Al a 790 gca Al a ctt Leu ccc Pr o ttg Leu 600 ttt Phe cgt Ar g ccg Pr o ctt Leu tcg Ser 680 agg Ar g ct c Leu gt g Val cca Pr o ggc G y 760 at g Met aca Thr gga G y t ca Ser 570 ttg Leu aat Asn cgc Ar g ttg Leu ttt Phe 650 act Thr cat Hi s cag G n gtt Val gat Asp 730 aaa Lys ttg Leu gt a Val tct Ser tac Tyr 555 gga G y at g Met tac Tyr at g Met gga G y 635 at g Met gca Al a gag G u gag G u cag G n 715 Al a cca Pr o ggt G y tgc Cys at t lie 795 ct g Leu gct Al a gag G u cgc Ar g ct g Leu 620 aat Asn aaa Lys ccg Pr o t ca Ser ct g Leu 700 gaa G u gcc Al a gat Asp gga G y gag G u 780 gct Al a cat Hi s agc Ser tgg Tr p aag Lys 605 cgt Ar g tct Ser cag G n act Thr cct Pr o 685 gca Al a gt a Val ttg Leu cag G n gca Al a 765 ccg Pr o at c l Ie cca Pr o cgt Ar g ct g Leu 590 ctt Leu gat Asp ccc Pr o ct g Leu cag G n 670 gat Asp cgc Ar g tgc Cys gat Asp cct Pr o 750 gaa G u gt a Val tgt Cys t ca Ser aaa Lys 575 tct Ser tct Ser gag G u t at Tyr ggt G y 655 gtt Val cgg Ar g at t SIle caa G n gcg Al a 735 ct g Leu ctt Leu gcc Al a gat Asp cgt Ar g 560 gcc Al a caa G n gat Asp ggc G y at t SIle 640 gat Asp gt g Val gcg Al a gcc Al a agc Ser 720 gaa G u gcc Al a gga G y ggt G y t cc Ser 800 545 gct Al a aag Lys aca Thr gct Al a gt a Val 625 tct Ser gcg Al a aaa Lys at c l Ie tct Ser 705 ttg Leu at c SIle aat Asn t ac Tyr gt g Val 785 at g let t at Tyr at t SIle gct Al a t cc Ser 610 gt g Val gaa G u gcg Al a gca Al a cag G n 690 gct Al a t ca Ser cgt Ar g at t SIle ggt G y 770 gaa G u cgg Ar g gaa G u cag G n gaa G u 595 t at Tyr ggg G y ct g Leu t ct Ser at c l Ie 675 gct Al a gat Asp ct a Leu gct Al a tct Ser 755 tct Ser gag G u tcg Ser cac Hi s gcg Al a 580 cca Pr o gat Asp cag G n at t SIle ggt G y 660 aag Lys gca Al a ttg Leu gt c Val gca Al a 740 gt g Val gat Asp cat Hi s agg Ar g ct g Leu 565 at g Met gat Asp cgc Ar g cgg Ar g at c SIe 645 cct Pr o gca Al a cga Ar g ct c Leu tgg Tr p 725 ctt Leu at c SIe gcc Al a gag G u ctt Leu 805 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 gcg cag cct tcc ggt gat cca cct ttg gag gtg gat ctg ggg ctg cgt Page 136 Ala G n Pro Ser cct Pr o aag Lys agg Ar g gag G u 870 cag G n ctt Leu gcg Al a gct Al a gaa G u 950 ctt Leu ct g Leu ggg G y t ac Tyr 840 gcg Al a at t SIle cgt Ar g cgc Ar g act Thr 920 gag G u ct g Leu gaa G u agg Ar g Sy 810 tct Ser aag Lys gtt Val cgt Ar g gtt Val 890 gct Al a at c SIle ccg Pr o aag Lys tgg Tr p 970 aag Lys ggt G y tgg Tr p gct Al a tt c Phe 875 cgt Ar g gat Asp gag G u gag G u cat Hi s 955 ct g Leu aga Ar g gcg Al a ggt G y ggt G y 860 cgc Ar g cga Ar g cga Ar g tgg Tr p ct g Leu 940 cag G n acg Thr tta Leu at t l Ie gaa G u 845 gat Asp t ac Tyr at t SIle aat Asn act Thr 925 cac Hi s at t SIle gca Al a gat Asp seql i st cor r ect ed. t xt Asp Pro Pro Leu G u Val Asp Leu gt g Val 830 act Thr cgt Ar g cca Pr o aag Lys acc Thr 910 gt g Val aat Asn at t SIle acg Thr cag G n 990 815 cgc Ar g tgg Tr p gag G u gtt Val gcg Al a 895 cat Hi s cag G n acg Thr aac Asn gct Al a 975 tta Leu acc Thr gag G u ct g Leu gac Asp 880 agg Ar g acc Thr ttg Leu tcg Ser cct Pr o 960 gct Al a cct Pr o gat Asp cag G n 850 at t SIle gca Al a gat Asp ct g Leu acc Thr 930 ttg Leu cag G n aat Asn cct Pr o t cc Ser 835 gcg Al a aag Lys acg Thr aat Asn ggt G y 915 at g let gaa G u gt g Val gcg Al a ggt G y 995 G y Leu Arg 820 t at Tyr ct g Leu ttc Phe cag G n gag G u 900 cgg Ar g at g Met gtt Val cag G n ctt Leu 980 ccg Pr o 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 3091 ctt gcg cag gtg gct ggt gcg tct Leu Ala G n Val Ala y Ala Ser 1000 1005 gag tat ttg gaa aac tat ctg aaa G u Tyr Leu G u Asn Tyr Leu Lys 1015 1020 gtt gat gaa gtc ttc tgg ggt gtg Val Asp G u Val Phe Trp y Val 1030 1035 taggtaggtg gtgggagccc caa <210> 100 <211> 1045 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 100 ggt tgg gat Sy Trp Asp gtg acc agg Val Thr Arg I gac tct atg Asp Ser IVet 1040 cca aat gag Pro Asn G u 1010 aag agt cgt Lys Ser Ar g 1025 gag caa cgt G u G n Ar g cag 3139 G n gtt 3187 Val ttt 3235 Phe 1045 3258 Page 137 l'bt Ser O31y Pr o Leu 1 5 seqi i st car r ect ed. t xt Ar g Ser O31u Ar g Lys Val Val (31y 10 Phe Val Ar g As p O31n Le u Le u Ar g Ar g ValI Ar g A a 145 As p Ou le Ly s As p 225 As n vb t ValI A a A a 305 Ser A a Ser Le u As p Tr p I Ie O31u Ph e As n 115 O31u Le u Le u O31u A a 1 95 O31y A a Tyr Ly s Ph e 275 Le u Ar g vb t Le u ValI 355 Ly s O31u Le u Le u As p Le u Le u Ph e ValI Thr 1 65 ValI As p Pr o A a O31u 245 O31y A a Ar g O31u Tyr 325 A a A a ValI Hi s Ser Tyr 70 O31n Le u O31n O31n O31u 150 Pr o Le u Le u ValI A a 230 Ly s A a O31u I Ie O31y 310 Tyr Ar g Le u O31y Le u O31y O31n O31u O31y Tr p A a 135 As p O31y Ly s A a Pr o 215 Le u Pr o O31n Pr a O31y 295 Ly s Ly s Pr a Ser Ser O31y 40 A a A a I Ie O31y Ly s 120 Le u Ph e Ph e Tr p O31y 200 Ph e Thr ValI O31u A a 280 Ser Ser Ar g vb t Pr a 360 Le u Tr p O31y Le u Ar g Ser 1 05 Le u Le u Ser Tyr Thr 1 85 Thr Ser A a As p Le u 265 As n As n O31y Tr p Thr 345 Le u Ser Ar g As p O31u O31n 90 Ser Le u O31u As p Thr 1 70 Tyr Tyr Thr A a Ser 250 As n Ser Ser A a A a 330 O31y Le u As n Pr a A a As p A a Ly s Ser A a 1 A a Ar g Pr a ValI Le u 235 A a Tyr Ly s Ph e Le u 315 O31u As p Ser O31u ValI O31y Ly s O31y As p 125 Ly s As n ValI Le u As p 205 vb t ValI Ser Ser Thr 285 O31u Ar g Tr p As n O31u Ser A a O31u Le u As p 110 A a A a I Ie Thr Le u 1 vb t O31n A a ValI As p 270 Ar g ValI Ser O31u Le u 350 Hi s Le u Le u As p Ar g Hi s Pr a O31n A a O31y 1 Thr Ar g Le u I Ie I Ie 255 ValI Thr As p Le u Ph e 335 O31y I Ie Tr p Th r Ala Se r (31n Arg9 365 Page 138 seql i st cor r ect ed. t xt Su Ser Phe Val Thr Asp Val G n Ala lMet 370 375 Asn 385 Gy Gy Hi s Leu Leu 465 lbet G u Leu Asn Al a 545 Al a Lys Thr Al a Val 625 Ser Al a Lys SIle Ser 705 Pr o Ar g SIle Leu 435 G u Ar g let Ser SIle 515 Val Lys G u G n G u 595 Tyr Sy Leu Ser SIle 675 Al a Asp Asp Val 405 G u Asp Tyr Lys Tr p 485 Al a Ser Asn G n Leu 565 let Asp Ar g Ar g SIle 645 Pr o Al a Ar g Leu Ar g Phe Leu Sy Phe 455 Thr Al a Al a Hi s Ser 535 Al a Al a Leu Sy Tr p 615 let Thr Leu Val Leu 695 let Asp Al a Ar g Tyr 440 Leu Hi s Ar g Met Ser 520 Al a Al a Leu Pr o Leu 600 Phe Ar g Pr o Leu Ser 680 Ar g Leu Ar g Val Val 425 Val Ar g Leu Al a G u 505 G n Asp Leu Al a Thr 585 Leu Leu SIle Asp Al a 665 Ar g Ar g Thr Arg Arg 380 Leu Lys 395 Leu Leu Ser Thr Arg G u Leu G u 460 Pro Lys 475 G y Phe Hi s Leu Phe Tyr li e Ar g 540 Tyr Leu 555 G y Al a let G u Tyr Arg IVet Leu 620 Sy Asn 635 let Lys Ala Pro Su Ser G u Leu 700 71n u 715 Arg Val Leu Asp Leu G n Val Asp 445 Hi s Pr o Thr Ar g Ar g 525 Leu Hi s Ser Tr p Lys 605 Ar g Ser G n Thr Pr o 685 Al a Val Ar g Val 415 Al a Hi s Leu Asp Ser 495 Val Leu Pr o Ser Lys 575 Ser Ser G u Tyr Sy 655 Val Ar g l Ie G n Sy 400 Hi s Leu Asn G n Ar g 480 let Ar g Leu Asp Ar g 560 Al a G n Asp Sy SIe 640 Asp Val Al a Al a Ser 720 Leu Ser Leu Val Trp Asp Ala Val Leu Asp Ala Ala Leu Asp Ala G u Page 139 seqi i st car r ect ed. t xt 730 Lys Pro Asp On I Ie As n I Ty r Val 785 lvb t As pI Val I Ie G31yl 865 31yj Val LysI Leu ThrI 945 Val Ar g ThrI Pr o Ly s 1 025 Ar g Ile (31y 770 (31u Ar g e u As p (31n 850 1le AJ a As p e u Th r 930 e u (31n As n Pr o As n 101 C A a Ser 755 Ser 31ui Ser 31yl Ser 835 A aI LysI Thr As n 31y, 915 lvb t 31u~ Val A aI 31yl 995 O31u AJ a 740 ValI As p HIi s Ar g Leu 820 Ty r Leu Ph e (31n (31u 900 Ar g lvbt ValI (31n Leu 980 Pr o Tyr Leu Asn Asp Pro I Ie A a O31u Le u 805 Ar g ValI Le u Le u A a 885 Ar g (31y Hi s Le u Thr 965 ValI G31y As p A a 790 A a Pr o Ly s Ar g O31u 870 O31n Le u A a A a O31u 950 Le u Le u ValI 775 ValI O31n O31u Tyr A a 855 Ser Le u Pr o Le u Hi s 935 ValI Ar g ValI O31n 745 Ar g Ph e Tr p Ser Ar g 825 O31u Tr p As p O31u O31y 905 As p I Ie O31u A a O31y 985 G31y cys I Ie 795 As p O31y Tr p A a Ph e 875 Ar g As p O31u O31u Hi s 955 Le u G31y O31u 780 A a Pr o A a O31y O31y 860 Ar g Ar g Ar g Tr p Le u 940 O31n Thr A a 765 Pr a I Ie Pr a I Ie O31u 845 As p Tyr I Ie As n Thr 925 Hi s I Ie A a Pro Leu Ala 750 o3 u Leu 03 y Val Ala O31y 0 ys As p Ser 800 Leu 03 u Val 815 Val Ar g Thr 830 Thr Tr p O31u Ar g O31u Leu Pr o Val As p 880 Lys Ala Ar g 895 Thr Hi s Thr 910 Val O31n Leu As n Thr Ser I Ie As n Pr o 960 Thr A a A a 975 O31n Leu Pr o 990 Lys Arg Leu Asp His Leu Ala O1n Val Ala O1y Ala Ser O31y Tr p Asp 1000 1005 O31n G31u Ty r Leu 1015 (31u Asn Tyr Leu Lys Val Thr Ar g 1 020 Se r Ar g O31n Val Val Asp O31u VaI Ph e Trp (31y VaI As p Ser [Vb t 1030 1035 1040 O31u O31n Ar g O31u Ph e 1 045 <210> 101 <21 1> 861 <212> DNA <21 3> Cor ynebact er i urn gi ut anm cumn Page 140 seql i st cor r ect ed. t xt <220> <221> CDS <222> <223> RXN03176 <400> 101 gag ttg gcc gat tac atc ccg gaa cta aaa tct gcg gac cca aac ccg 48 G u Leu Ala Asp Tyr Ile Pro u Leu Lys Ser Ala Asp Pro Asn Pro 1 5 10 ctg gca gta gcc ctg tgc acc gtt aac gga cac atc tac agc gca ggc 96 Leu Ala Val Ala Leu Cys Thr Val Asn G y His I I e Tyr Ser Ala G y 25 gat gac gac atc gaa ttc acc atg caa agt att tcc aag cca ttt gcc 144 Asp Asp Asp Ile G u Phe Thr IVet G n Ser Ile Ser Lys Pro Phe Ala 40 t ac gca ctc gca ctc caa gaa tgc ggc ttt gat gag gt c tct gca tcc 192 Tyr Ala Leu Ala Leu n G u Cys y Phe Asp G u Val Ser Ala Ser 55 gtg gcc ttg gag ccc tcc ggt gag gcc ttc aac gaa ctt tcc ctc gac 240 Val Ala Leu G u Pro Ser Gy G u Ala Phe Asn G u Leu Ser Leu Asp 70 75 ggc gaa aac cgc ccc atg aac ccc atg atc aac gcc ggc gcg atc gcc 288 Sy G u Asn Arg Pro Iet Asn Pro Iet Ile Asn Ala y Ala ile Ala 90 atc aac cag ctg atc aac ggc tcc gat tcc acc gtg gaa gac cgc gtg 336 Ile Asn G n Leu Ile Asn y Ser Asp Ser Thr Val G u Asp Arg Val 100 105 110 gaa aaa atc cga cac tac ttc tct gaa ctt gct gga cgc gaa ctc acc 384 G u Lys lie Arg His Tyr Phe Ser G u Leu Ala G y Arg G u Leu Thr 115 120 125 atc gac cgc gtg ctt gcc gaa tcc gaa ctc gcc ggc gcc gac cgc aac 432 Ile Asp Arg Val Leu Al a u Ser G u Leu Ala y Ala Asp Arg Asn 130 135 140 ctc tcc atc gcc cac atg ctg cgc aat tac ggc gtc atc gaa gac gaa 480 Leu Ser lie Ala His Iet Leu Arg Asn Tyr G y Val Ile G u Asp G u 145 150 155 160 gcc cac gac gcc gtc ctc agc tac acg ctg caa tgc gcc atc aaa gta 528 Ala His Asp Ala Val Leu Ser Tyr Thr Leu G n Cys Ala lie Lys Val 165 170 175 acc acg cgc gac ctc gca gtc atg acc gcc acg ctc gcc gcc ggc ggc 576 Thr Thr Arg Asp Leu Al a Val IVet Thr Al a Thr Leu Al a Al a G y G y 180 185 190 aca cac cca att acc ggc aag aag ctt ctc gac gcc cgc gtc tgc cgc 624 Thr His Pro lie Thr G y Lys Lys Leu Leu Asp Ala Arg Val Cys Arg 195 200 205 ctc acc ctc tcc gtc atg gct tca gca ggc atg tac gac gag gca ggg 672 Leu Thr Leu Ser Val IVet Ala Ser Ala Gy IVet Tyr Asp G u Ala y 210 215 220 cag tgg ctc tcc acc gta ggc atc ccc gcg aaa tca gga gtc gcc ggc 720 G n Trp Leu Ser Thr Val Gy Ile Pro Ala Lys Ser y Val Ala y 225 230 235 240 gga ctc atc ggc att ctg cca ggt cag ctg ggc atc gcc aca ttt tcc 768 Sy Leu lie Gy lie Leu Pro Gy n Leu Gy Ile Ala Thr Phe Ser Page 141 cca cgc ct g aac Pr o Ar g Leu Asn 260 aaa cag ctt t cc Lys 03 n Leu Ser 275 245 ccc Pr o g ac As p seqI i st car r ect ed. t xt 250 255 aaa ggc aac agc gtg cgc ggc gta aaa ata ttc Lys G31y Asn Ser Val Ar g G31y ValI Lys I Ie Phe 265 270 gac at g ggc ct C cac ct c at g t cc acc gag As p I'bt G31y Leu Hi s Leu I'bt Ser Thr O31u 280 285 <210O> 102 <21 1> 287 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 102 Ou Leu Ala Asp Tyr Ilie Pro Ou Leu Le u As p Tyr ValI G31y I Ie O31u I Ie Le u 145 A a Thr Thr Le u O31n 225 G31y Pr o ValI As p Le u Le u As n O31n I Ie 115 Ar g I Ie As p Ar g Pr o 1 95 Le u Le u I Ie Le u Le u O31u Le u Pr o Pr a li1e Hi s Le u Hi s ValI 1 65 Le u Thr ValI Thr I Ie 245 Pr a Cy s Ph e O31n Ser 70 vb t As n Tyr A a vb t 150 Le u A a G31y vb t ValI 230 Le u Lys5 Thr Thr (31u G31y As n G31y Ph e (31u 135 Le u Ser ValI Lys5 A a 215 G31y Pr a G31y As n O31n G31y A a Nibt As p 1 05 O31u O31u As n Thr Thr 1 85 Le u A a Pr a O31n Ser 265 Lys5 10 O31y Ser Ph e Ph e I Ie 90 Ser Le u Le u Tyr Le u 1 70 A a Le u O31y A a Le u 250 Ser Ala Asp Pro Asn Pro Hi s I Ie As p As n 75 As n Thr A a A a O31y 1 55 O31n Thr As p vb t Lys5 235 (31y Tyr Lys5 ValI Le u O31y O31u Ar g 125 A a I Ie A a A a Ar g 205 As p O31y A a Se r A a 03 y Pr o Phe Ala Ser A a Ser Ser Leu Asp A a IlIe A a Asp Ar g Val 110 O3 u Leu Thr Asp Arg Asn 31u Asp (1u 1 Ile Lys Val 1 A a 03 y 03 y 1 Val Cys Ar g O3 u A a 03 y Val Ala (31y 240 Thr Phe Ser 255 Lys Il e Phe 270 Val Arg Oy Val Page 142 seqI i st car r ect ed. t xt Lys O31n Leu Ser Asp Asp I'bt G31y Leu Hi s Leu I'bt Ser Thr (31u 275 280 285 <210> 103 <211> 861 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (861) <223> FRXA02879 <400> 103 gag t tg gcc 03 u Leu Al a ct g gca gt a Leu Al a Val gat gac gac Asp Asp Asp t ac gca ct c Tyr Al a Leu gt g gcc t tg Val Al a Leu ggc gaa aac (31y 1u Asn atc aac cag Ile Asn 03 n gaa aaa atc 03 u Lys IlIe 115 at c gac cgc Ilie Asp Ar g 130 ct c t cc at c Leu Ser Ilie 145 gcc cac gac Al a Hi s Asp acc acg cgc Thr Thr Ar g aca cac cca Thr Hi s Pr o 1 95 t ac Tyr ct g Le u g aa (31u ct c Le u CCC Pr o CCC Pr o at c I Ie c ac Hi s ct t Le u c ac Hi s gt c ValI 1 65 ct c Le u ac c Thr at c ccg gaa ct a Ilie Pro Ou Leu t gc Cy s t t c Ph e caa O31n t cc Ser 70 at g vb t aac As n t ac Tyr gcc Al a at g vb t 150 ct c Le u gca Al a ggc G31y ac c Thr ac c Thr g aa O31u 55 ggt G31y aac As n ggc G31y tt c Ph e g aa O31u 135 ct g Le u ag c Ser gt c ValI aag Lys5 aac As n c aa O31n G31y gcc Al a at g vb t g at As p 1 05 g aa (31u g aa (31u aat As n ac g Thr ac c Thr 1 85 ct t Le u aaa Lys5 10 gga G31y agt Ser tt t Ph e tt c Ph e at c I Ie 90 t cc Ser ct t Le u ct c Le u t ac Tyr ct g Le u 1 70 gcc Al a ct c Le u t ct gcg gac cca aac ccg Ser Ala Asp Pro Asn Pro c ac Hi s at t I Ie g at As p aac As n 75 aac As n ac c Thr gct Al a gcc Al a ggc G31y 1 55 c aa O31n ac g Thr g ac As p at c I Ie t cc Ser gag O31u g aa O31u gcc Al a gt g ValI gga O31y ggc O31y 140 gt c ValI t gc cys ct c Le u gcc Al a t ac Tyr aag Lys5 gt c ValI ct t Le u ggc O31y g aa O31u cgc Ar g 125 gcc Al a at c I Ie gcc Al a gcc Al a cgc Ar g 205 ag c Ser cca Pr o t ct Ser t cc Ser gcg Al a g ac As p 110 g aa O31u g ac As p g aa O31u at c I Ie gcc Al a 1 gt c ValI gca Al a tt t Ph e gca Al a ct c Le u at c I Ie cgc Ar g ct c Le u cgc Ar g g ac As p aaa Lys5 1 ggc O31y t gc Cys ggc O31y gcc Al a t cc Ser g ac As p gcc Al a gt g ValI ac c Thr aac As n g aa O31u 1 gt a ValI ggc O31y cgc Ar g ctc acc ctc tcc gtc at g gct tca gca ggc atg tac gac gag gca ggg Page 143 Le u c ag O31n 225 gga G31y cca Pr o aaa Ly s Le u ct c Le u at c I Ie ct g Le u ct t Le u 275 ValI ac C Thr at t I Ie 245 CCC Pr o g ac As p vb t gt a ValI 230 ct g Le u aaa Ly s gac As p A a 215 ggC G31y cca Pr o G31y at g vb t seql i st car r ect ed. t xt Ser A a 03 y I'bt Ty r As p 220 at c ccc gcg aaa t ca gga I Ie Pro Ala Lys Ser (31y 235 ggt cag ctg ggc atc gcc 03 y 03 n Leu 03 y Il e A a 250 aac agc gt g cgc ggc gt a Asn Ser Val Ar g G31y Val 265 ggc ct c cac ct c at g t cc G31y Leu Hi s Leu I'b t Ser 280 285 G31u gt c ValI ac a Thr aaa Lys5 270 ac C Thr A a gcc A a tt t Ph e 255 at a I Ie gag (31u <210O> 104 <21 1> 287 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 104 03 u Leu A a Asp Leu Ala Val Ala Asp Asp Asp I I e Ty r A a Leu A a Val Ala Leu (31u G31y (31u As n Ar g I I e As n 03 n Leu 1 00 031u Lys I Ie Ar g 115 li1e As p Ar g Val 130 Leu Ser I Ie Ala 145 A a Hi s As p A a Thr Thr Ar g As p 1 80 Thr Hi s Pr o I Ie 1 95 Leu Thr Leu Ser 210 Ty r Le u (31u Le u Pr o Pr a li1e Hi s Le u Hi s ValI 1 65 Le u Th r ValI Ilie Pro 0u Leu Ser Ala Asp Pro As n Pr o Cy s Ph e O31n Ser 70 vb t As n Ty r A a vb t 150 Le u A a G31y vb t Th r Th r O31u 55 G31y As n G31y Ph e O31u 135 Le u Ser ValI Lys5 A a 215 As n 25 O31n G31y A a Nibt As p 1 05 (31u (31u As n Th r Th r 1 85 Le u A a O3 y Hi s Ser I Ie Phe As p Phe As n 75 1 1 e As n 90 Ser Thr Leu A a Leu A a Ty r 03 y 1 55 Leu 03 n 1 70 A a Thr Leu Asp 31y Ib t I Ie Ser 031u 031u A a ValI G31y G31y 140 ValI cys Le u A a Ty r 220 Ty r Lys5 ValI Le u G31y 031u Ar g 125 A a I Ie A a A a Ar g 205 As p Ser Pr a Ser Ser A a As p 110 031u As p 031u I Ie A a 1 ValI (31u A a Ph e A a Le u I Ie Ar g Le u Ar g As p Lys5 1 G31y cys A a G31y A a Ser As p A a ValI Th r As n 031u 1 ValI G31y Ar g (31y Page 144 seql i st cor r ect ed. t xt G n Trp Leu Ser Thr Val Gy Ile Pro Ala Lys Ser Gy Val Ala y 225 230 235 240 Sy Leu lie Gy lie Leu Pro Gy n Leu y I e Ala Thr Phe Ser 245 250 255 Pro Arg Leu Asn Pro Lys y Asn Ser Val Arg y Val Lys lie Phe 260 265 270 Lys G n Leu Ser Asp Asp IVet G y Leu Hi s Leu IVet Ser Thr G u 275 280 285 <210> 105 <211> 1155 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1132) <223> RXA00278 <400> 105 ggccacggag ttggtgtccg tcatggtgat gattctgtct gtgggtcggc atccgccccc gtttgcagga gtacttataa gtcgtttctg tgttggcttt atg cac gct ttt Met His Ala Phe cgc Ar g acg Thr ttg Leu tcg Ser aat Asn gt a Val tcg Ser ggt Gy gac Asp cgt Ar g cct Pr o ctt Leu gct Al a gaa G u cca Pr o ggc Gy aac Asn gaa G u 120 aat Asn ctt Leu ct c Leu t cc Ser gat Asp ccc Pr o tct Ser gt c Val ct c Leu gaa G u gt g Val caa G n acc Thr tgc Cys ccc Pr o ggt G y tta Leu 75 aac Asn agt Ser tta Leu gat Asp ggt G y acg Thr act Thr gat Asp gaa G u cgc Ar g cgg Ar g ttc Phe gcg Al a ttc Phe 140 gat Asp cga Ar g cca Pr o gcc Al a 45 aaa Lys ccg Pr o ggt Sy cgt Ar g agg Ar g 125 cga Ar g gt a Val gtc ggc Val G y 15 aca cct Thr Pro ggt cca G y Pro gag ccc G u Pro gat gat Asp Asp cgc at c Arg Ile 95 gat ccg Asp Pro 110 gaa att G u Ile ttc gtc Phe Val gat at t Asp Il e gct Al a gt g Val cca Pr o at t SIle ct g Leu 80 at t l Ie gt g Val t cc Ser ggc Sy gt g Val 1 gca Al a gaa G u ct g Leu gt g Val acc Thr gt g Val act Thr cgc Ar g tcg Ser 145 ttg Leu ccg Pr o cca Pr o gaa G u cct Pr o ggt Sy ggt Sy gac Asp 130 t cc Ser ct g Leu gca Al a ccg Pr o gt a Val gag G u gt g Val gag G u 115 at t SIle gac Asp gcc Al a gaa G u gat Asp at a SIle gag G u gat Asp 100 ct g Leu ttc Phe cgt Ar g cga Ar g gca Al a acc Thr tct Ser gag cgg Ar g caa G n cgc Ar g ggt Sy ctg Leu att cgt tcc gtc acg lie Arg Ser Val Thr Page 145 seql i st cor r ect ed. t xt at c SIle cgc Ar g at c SIle t ca Ser act Thr 230 cag G n gat Asp gga Sy at t SIle tgg Tr p 310 ggt Gy gat Asp gac gaa cgc Asp u Arg 170 caa acc cgc G n Thr Arg 185 gat ct a ccc Asp Leu Pro 200 cag cga gcc G n Arg Ala aat tgg t cc Asn Trp Ser att ttg ggc Ile Leu G y 250 tac acc gag Tyr Thr G u 265 gca gcg gca Al a Al a Al a 280 cag gta aac Sn Val Asn aca atg ttc Thr Met Phe cca cag ct g Pro G n Leu 330 gga taatgaa! Gy gcc Al a at g Met ggc Sy cgc Ar g gat Asp 235 gat Asp at t SIle tcg Ser tac Ty r gac Asp 315 cag G n aaa Lys ttg Leu cac Hi s acc Thr 220 tgt Cys gat Asp ctt Leu acc Thr aca Thr 300 gat Asp t ac Tyr gt g Val at g Met 190 at t SIle gcg Al a at g Met at t SIle acc Thr 270 gct Al a gaa G u ttc Phe cca Pr o gaa G u 175 gaa G u tgt Cys ccg Pr o gcg Al a ttg Leu 255 t cc Ser gaa G u cgg Ar g gga Gy gag 33u 335 160 ttt Phe tct Ser gt c Val gag G u ttg Leu 240 t cc Ser ct c Leu aca Thr at c SIle cct Pr o 320 aca Thr gga Gy gat Asp 210 t ca Ser cag G n at c SIle t cc Ser tct Ser 290 aca Thr ct c Leu t ac Tyr 180 acg Thr t cc Ser tta Leu cag G n gca Al a 260 t cc Ser ggg G y cgc Ar g t cc Ser ct g Leu t cc Ser act Thr aaa Lys gct Al a 245 cag G n t at Tyr ttg Leu at g Met t ac Tyr 325 643 691 739 787 835 883 931 979 1027 1075 1123 1155 gaa gaa ggg aca gaa aac G u G u G y Thr G u Asn 340 gat ttcgatccag at t <210> 106 <211> 344 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 106 IVet His Ala Phe Arg Arg Pro Pro Pro Leu Thr Thr Arg Val y Ala 1 5 10 Ala Leu Leu Ala Ala Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val 25 G u Pro Ala G u Thr Leu Thr Ala Leu Asp Pro Asp Ala y Pro Pro 40 Leu Pro Pro Asp Ser Ser iIe G u Ala Pro G y G u Lys u Pro lie 55 Page 146 seqi i st car r ect ed. t xt Val Ou Val Ilie OuAsn Trp Pro G1y Ser Leu Arg Pro Asp Asp Leu Thr ValI Thr Ar g Ser 145 li1e Ser Ser Thr A a 225 O31n G31y As p As p Ar g 305 Tyr (31u O31u ValI O31u 115 li1e As p Ser Pr o I Ie 1 95 Gy le Hi s A a Hi s 275 Ser As p Tr p Thr Ar g O31n Ar g O31y Le u Thr 1 65 Le u Ser Thr Ly s A a 245 O31n Tyr Le u vb t Tyr 325 As n Pr o O31n Ph e Pr o 135 Ser Thr Thr A a Le u 215 Ar g ValI Pr o ValI Ar g 295 Tr p Pr o (31u I Ie Le u 1 ValI Ly s As p O31u Thr 1 Le u Ar g Tr p Le u Thr 265 A a ValI vb t O31n As n Ser Le u As p O31y 1 A a vb t (31y Ar g As p 235 As p I Ie Ser Tyr As p 315 O31n O31y Ar g Ar g 125 Ar g ValI Le u Thr Thr 205 li1e Le u ValI As p Thr 285 li1e Tr p vb t lIe Pr a I Ie ValI I Ie O31u 1 O31u cys Pr a A a Le u 255 Ser O31u Ar g O31y O31u 335 <210O> 107 <21 1> 1035 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1012) <223> RXA00727 <400> 107 t tat tgcacc at ggcaggt a gt att t tgcc cat gat tgag agt t tcaccc gat tgcgaat Page 147 seql i st cor r ect ed. t xt ttcgcagaca ccgcctctga aacggctact gcat agcact at c l Ie ttc Phe ggc G y gtt Val cca Pr o ggt Gy gag G u caa G n gaa cag G n 150 gcc Al a gac Asp ccg Pr o ttg Leu gct Al a 230 at g M\et aag Lys ttg Leu gag Su gaa G u ttc Phe gaa G u agc Ser ggc G y 120 cgc Ar g gcg Al a ggt Sy gt c Val acc Thr 200 ct g Leu gct Al a ttg Leu gct Al a tct Ser at c SIle ct g Leu ccg Pr o gac Asp cag G n ctt Leu aac Asn gca Al a acc Thr 170 cct Pr o ttg Leu cgc Ar g gag G u gcg Al a 250 gt a Val tgt Cys gtt Val gct Al a ttt Phe 75 ct c Leu gag G u gat Asp ttt Phe act Thr 155 gtt Val cgc Ar g tct Ser gca Al a cgc Ar g 235 cca Pr o gcg Al a gt c Val cca Pr o cag G n gag G u gt g Val cag G n at c SIle gat Asp 140 gat Asp gct Al a t ca Ser t at Tyr gac Asp 220 t cc Ser ttt Phe gcg Al a acc Thr gat Asp 45 cgc Ar g ttt Phe cgt Ar g gat Asp gga G y 125 ttc Phe cgt Ar g gt a Val gct Al a gaa G u 205 gca Al a gaa G u ggt Gy acc Thr aat Asn cct Pr o ggt G y aaa Lys gca Al a ttc Phe 110 gcc Al a at c SIle gaa G u cag G n caa G n 190 acc Thr ctt Leu G y ttc Phe gt c Val 15 gag G u gt a Val gt g Val gat Asp at g Met 95 t cc Ser tct Ser gat Asp acc Thr cgc Ar g 175 tgt Cys gca Al a gct Al a cgc Ar g gca Al a 255 ggc G y gag G u ccg Pr o ct c Leu t cc Ser 80 gcg Al a ct c Leu ggc G y ttc Phe cca Pr o 160 aca Thr gaa G u gat Asp gcg Al a at c SIle 240 ttc Phe ttg cgt ttc Leu Arg Phe 1 atc gtg gca Ile Val Al a cag ggc aac n G y Asn gag att cag G u lie G n acc gcc ggt Thr Al a G y gat ggt cag Asp y n ggg gtg at g G y Val Mbet atc ctt cca Ile Leu Pro 115 ttc act gac Phe Thr Asp 130 ctc ttc gca Leu Phe Al a 145 at c gat ccg IIe Asp Pro acc gtg gca Thr Val Al a gcc gaa ggc Al a G u G y 195 act gca gct Thr Al a Al a 210 gac t cc cct Asp Ser Pro 225 gaa gt t gtg G u Val Val ccg tt g gaa Pro Leu G u ccc Pr o acc Thr cca Pr o gcg Al a gcc Al a at t SIle ggc G y 100 tcg Ser aac Asn ggt G y gaa G u gag G u 180 aaa Lys acc Thr gtt Val ggc G y tct Ser 260 aaa Lys t ca Ser gat Asp at g Met aac Asn at c SIe ttg Leu gtt Val gag G u gtg Val aac Asn 165 acc Thr gag G u gca Al a t ca Ser gat Asp 245 gac Asp Page 148 seql i st cor r ect ed. t xt ctc acc cca gca gca gct gcg gcg ttc caa cac ttg att gac acc ggc 931 Leu Thr Pro Ala Ala Al a Ala Ala Phe G n His Leu IIe Asp Thr G y 265 270 275 gat tac cag cgc atc atg gcg caa tgg ggc att gaa gaa ggc ctt ctt 979 Asp Tyr Gn Arg lie IVet Ala Gn Trp G y lie G u G u G y Leu Leu 280 285 290 gat gag gcc ctg atc aac gaa cag cca ctc aac tagagccttc cagcaactaa 1032 Asp G u Ala Leu Ile Asn G u n Pro Leu Asn 295 300 aaa 1035 <210> 108 <211> 304 <212> PRT <213> Cor ynebact eri um gl ut ari cum Leu 1 I e G n G u Thr Asp Sy SIle Phe Leu 145 I le Thr Al a Thr Asp 225 0> 108 Arg Phe Val Ala Sy Asn Ile G n Al a G y G y G n Val Vbet Leu Pro 115 Thr Asp 130 Phe Al a Asp Pro Val Ala G u G y 195 Al a Al a 210 Ser Pro Pro Lys Ile Pro Lys 5 Ser Asp Met Asn SIle Leu Val G u Val Asn 165 Thr G u Al a Ser Phe Sy Val Pr o 70 Sy G u G n G u G n 150 Al a Asp Pr o Leu Al a 230 Thr Tr p Pr o 55 Pr o Val Phe Al a Ar g 135 Tr p Cys Asp SIle SIle 215 Tr p Arg Ala 10 Al a Ser Gn lle Al a Leu Pro Pro IVet Asp 90 Pro G n 105 Thr Leu Su Asn G n Al a Leu Thr 170 Arg Pro 185 II e Leu G y Arg Al a G u Val Ala Ala Thr Val y Cys Val Al a Phe Leu G u Asp Phe Thr 155 Val Ar g Ser Al a Ar g 235 Val Pr o G n G u Val G n SIle Asp 140 Asp Al a Ser Tyr Asp 220 Ser Thr Asp Ar g Phe Ar g Asp Sy 125 Phe Ar g Val Al a G u 205 Al a G u Asn Pr o Sy Lys Al a Phe 110 Al a SIle G u G n G n 190 Thr Leu Sy G u Val Val Asp bet Ser Ser Asp Thr Ar g 175 Cys Al a Al a Ar g Su Val Val Gy Asp Mebt Tyr Leu Ala Ala Pro Phe y Phe Ala Phe Page 149 seql i st cor r ect ed. t xt 245 250 255 Pro Leu u Ser Asp Leu Thr Pro Ala Ala Ala Ala Ala Phe G n His 260 265 270 Leu Ile Asp Thr y Asp Tyr n Arg lie Met Ala n Trp Gy lie 275 280 285 G u G u G y Leu Leu Asp G u Al a Leu Ile Asn G u n Pro Leu Asn 290 295 300 <210> 109 <211> 1962 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1939) <223> RXA02139 <400> 109 tcaggaggat ttttcaatca tgtgcggcct tcttggcata tgaagcattc gttcctgcac tcgagcgggc cttgccatgc ttgactgcaa atgggaacgc cct Pr o aac Asn tgg Tr p gag G u t at Tyr cac Hi s at t SIle cag G n gt g Val gat Asp ct c Leu cct Pr o t ac Tyr ttt Phe tgg Tr p at t SIle ggc Gy 120 t cc Ser ggc Gy at c SIle gat Asp t ac Tyr act Thr gag G u gat Asp aag Lys gag G u act Thr at t SIle gaa G u gtt Val tct Ser 75 t cc Ser aca Thr cca Pr o aag Lys tgg Tr p gat Asp ccc Pr o gag G u ggc Gy gt g Val aag Lys ct g Leu aag Lys 140 gac Asp gca Al a cgc Ar g cgt Ar g ggc Gy gag G u aag Lys 110 t ac Tyr at c SIle gcc Al a 15 cac Hi s t ac Tyr aaa Lys gag G u cat Hi s 95 tcg Ser gca Al a ttg Leu gat Asp t cc Ser gca Al a gag G u cca Pr o ct c Leu ctt Leu acc Thr gag G u at g Met 1 gca Al a cac Hi s at g Met ct c Leu at t SIle cgc Ar g ttc Phe acc Thr at g Met 145 cgc cac cgt Arg His Arg gcg Al a caa G n act Thr tcg Ser gtt Val gga Gy ctt Leu gag G u 130 gcc Al a ttt Phe cca Pr o ttc Phe gat Asp gt c Val at g Met gcg Al a 115 cat Hi s gag G u gga Gy ctg Leu aac Asn ttg Leu ggt Gy ttc Phe 100 cgt Ar g ggc gagy gag G u Page 150 seql i st cor r ect ed. t xt aat cta gat ctg ggc ctt gat Asn Leu Asp Leu G y Leu Asp 150 155 aag cgc acc Lys Arg Thr ttg Leu ttg Leu cag G n ggt Gy agc Ser 230 ttc Phe aac Asn tcg Ser cac Hi s aag Lys 310 ccg Pr o ct g Leu aag Lys cgt Ar g ggc Gy 390 t ac Tyr t ac Tyr t ca Ser cgt Ar g 200 gag G u gaa G u ggc Gy gac Asp gt c Val 280 gt g Val at g Met t ac Tyr ggc Gy ccg Pr o 360 ggc Gy t cc Ser aac Asn ccc Pr o 170 tgc Cys ttc Phe gac Asp cat Hi s gac Asp 250 ct c Leu gt g Val at t SIle t ac Tyr gt g Val 330 ggc Gy tcg Ser gga Gy ctt Leu cgc Ar g 410 gag G u acc Thr aag Lys ct c Leu at g bet 235 t ca Ser acc Thr gct Al a gt c Val tt g Leu 315 gca Al a gca Al a ctt Leu aag Lys gag G u 395 t cc Ser cca Pr o gca Al a cct Pr o ttc Phe 220 cgt Ar g acc Thr ttc Phe gcg Al a tcg Ser 300 gat Asp gcg Al a gat Asp gct Al a ct c Leu 380 cgt Ar g ttc Phe gat Asp aca Thr cag G n 205 gat Asp gcc Al a gca Al a acc Thr gag G u 285 cct Pr o gat Asp gaa G u gag G u cca Pr o 365 agc Ser ggc Gy aat Asn acc Thr gtt Val 190 ttc Phe cgc Ar g gac Asp at t l Ie acc Thr 270 t cc Ser gag G u cct Pr o gca Al a ct g Leu 350 ttt Phe aag Lys t cc Ser ttc Phe ctt Leu 175 cgt Ar g cca Pr o at t SIle gt g Val gcg Al a 255 ggt Gy gcc Al a gaa G u gt a Val cgt Ar g 335 ttc Phe gag G u gtt Val at g Met gag 15u 415 att gag Ile G u 160 cac gcg Hi s Al a ccg ggc Pro G y gta cag Val G n gcc cag Al a G n 225 acc gt a Thr Val 240 gcg ctt Al a Leu ttc gag Phe G u gct gcg Al a Al a tac gcc Tyr Al a 305 gct gac Al a Asp 320 aag cac Lys Hi s ggt gga G y G y aag at c Lys II e ctg cca Leu Pro 385 acc at g Thr Mbet 400 cag atg G n Met cac tac gtg gac His Tyr Val Asp 165 cag att tcc cgc Sn Ile Ser Arg 180 ggc aag ctg gaa G y Lys Leu G u 195 aag gtc gta aag Lys Val Val Lys 210 gtg ttg gag gat Val Leu G u Asp ggc tcg ttc ctt G y Ser Phe Leu 245 gca aag cgc cac Ala Lys Arg His 260 cgt gaa ggc tac Arg G u G y Tyr 275 att ggc gct gag lle G y Al a u 290 aac gcg att cct Asn Ala lie Pro cca tca ttg gtc Pro Ser Leu Val 325 gtc aag gtt gtg Val Lys Val Val 340 tac acc att tac Tyr Thr Ile Tyr 355 cct tcc cca cta Pro Ser Pro Leu 370 gac ggc atg aag Asp Gy IVet Lys gaa gag cgc tac G u G u Arg Tyr 405 caa cgc gtt att Sn Arg Val lle 420 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 Page 151 seql i st cor r ect ed. txt cca tgg gca aag cgc gaa tgg gac cac cgc gaa gtc act Pro Trp Ala Lys Arg Gu Trp Asp His Arg u Val Thr 425 430 gcg ccg atc Ala Pro Ile t ac Ty r gat Asp at c SIle 470 gaa Gu aac Asn ccg Pr o cgc Ar g at c SIle 550 gat Asp ct g Leu aac Asn gca cag t cc Al a G n Ser 440 ctg ttc acc Leu Phe Thr 455 aac atg gcg Asn IVet Ala gtt ttc aag Val Phe Lys ggt acc acc G y Thr Thr 505 cct cac gtt Pro His Val 520 cac tgg ct t His Trp Leu 535 aag gaa tcc Lys G u Ser at g ct g aac IVet Leu Asn tgg act gtt Trp Thr Val 585 cgc att gat Arg lie Asp 600 cgc aac Arg Asn tgg atg Trp Vbet aac t cc Asn Ser 475 gtt gca Val Ala 490 aag t ac Lys Tyr ttg cac Leu Hi s gcc ggc Al a G y ggt act Sy Thr 555 gag cac G u Hi s 570 ctg tca Leu Ser cca cag Pro G n ttt Phe cgc Ar g 460 ctt Leu gag G u gcg Al a cgc Ar g gat Asp 540 gaa G u cgc Ar g ttt Phe at t SIle cca Pr o gac Asp ct g Leu at t SIle cgc Ar g 510 aag Lys ct g Leu at c SIle ggc Sy gt g Val 590 gac Asp gt a Val at c SIle cga Ar g cct Pr o 495 agg Ar g ct g Leu ttc Phe ttc Phe gt g Val 575 tgg Tr p cgc Ar g gcc Al a ct g Leu gtt Val 480 t ac Tyr gca Al a ggc Gy ggt Sy aac Asn 560 t ca Ser cac Hi s t cc Ser at g NMbt 450 aag Lys ttc Phe ct g Leu gag G u cct Pr o 530 gcg Al a cag G n cat Hi s at t SIle cca Pr o 610 435 caa G n gct Al a ttg Leu aag Lys cag G n 515 gtt Val cag G n gct Al a t cc Ser ttt Phe 595 gt c Val cac Hi s gac Asp gat Asp at t SIle 500 at t SIle ccc Pr o gac Asp gt g Val cgt Ar g 580 gt g Val gag a u ct g Leu aag Lys aag Lys 485 gcc Al a gtt Val at g Met acc Thr ct g Leu 565 cga Ar g gaa G u ctt Leu 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1962 taagtct t aa agcct aaacc ccc <210> 110 <211> 613 <212> PRT <213> Corynebacter i um gl ut am cum <400> 110 IVet Arg His Arg y Pro Asp Asp Ala y Thr Trp His Asp Ala Asp 1 5 10 Ala Ala Phe G y Phe Asn Arg Leu Ser lie lie Asp lie Ala His Ser 25 His G n Pro Leu Arg Trp G y Pro Ala Asp G u Pro Asp Arg Tyr Ala 40 Iet Thr Phe Asn G y G u lie Tyr Asn Tyr Val G u Leu Arg Lys G u 55 Page 152 seqI i st car r ect ed. t xt Leu Ser Asp Leu 03 y Al a Phe Asn Thr Ser 3y Asp Gy Ou Pro li1e Ar g Ph e Thr vb t 145 O31u Al a G y O31n O31n 225 ValI Le u O31u Al a Al a 305 As p Hi s G31y I Ie Pr o 385 vb t ValI Nibt Al a 115 Hi s O31u Tyr I Ie Ly s 1 95 ValI Le u Ser Ly s O31u 275 G31y Al a Ser Ly s Thr 355 Ser G31y O31u Ph e G31y As p Thr vb t As p 1 65 Ar g G u Ly s As p Le u 245 Hi s Tyr O31u Pr o ValI 325 ValI Tyr Le u Ly s Tyr 405 Hi s Al a Ph e Ph e 135 Le u O31n (31u Ly s Ly s 215 ValI G31y Pr o (31u I Ie 295 li1e Le u Ser (31u Ly s 375 Ly s G31y G31y Tr p 1 li1e Ser Le u ValI G31y 1 Tyr O31n Ly s I Ie Le u 265 As p Ly s Tr p Ph e O31u 345 Le u Le u Le u Al a Ser Thr Pr o Ly s Le u 1 O31u Thr Ly s Le u vb t 235 Ser Thr Al a ValI Le u 315 Al a Al a Le u Ly s O31u 395 Ser ValI Ly s Le u Ly s 140 As p Pr a Al a Pr a Ph e 220 Ar g Thr Ph e Al a Ser 300 As p Al a As p Al a Le u 380 Ar g Ph e ValI O31u Ph e 125 Thr Ly s As p Thr G n 205 As p Al a Al a Thr O31u 285 Pr a As p O31u O31u Pr a 365 Ser O31y As n Hi s Ser Al a Le u Thr Le u 1 Ar g Pr a I Ie ValI Al a 255 O31y Al a O31u ValI Ar g 335 Ph e O31u ValI vb t O31u 415 I'bt On Arg Val Ilie Pro Trp Ala Lys Arg Ou Trp Asp His Arg Ou Page 153 seqI i st car r ect ed. t xt 420 Pro lieTyr Ala ValI Ar g ValI 465 Pr o As p Le u Ph e Tr p 545 Ly s As p G31y Tyr Al a 435 (31n Al a Le u Ly s O31n 515 ValI O31n Al a Ser Ph e 595 ValI Le u Ly s Ly s 485 Al a ValI vb t Thr Le u 565 Ar g O31u Le u Le u 455 As n ValI G31y Pr o Hi s 535 Ly s vb t Tr p Ar g 425 O3 n Se r 440 Phe Thr [Vb t Al a Phe Lys Thr Thr 505 Hi s Val 520 Trp Leu O3 u Ser Leu Asn Thr Val 585 1 1 e As p 600 Arg Asn Phe 430 Pro Val Al a Ser 475 Al a Tyr Hi s G31y Thr 555 Hi s Ser O31n Ar g 460 Le u O31u Al a Ar g As p 540 O31u Ar g Ph e I Ie As p 445 G31y O31u Thr Le u Ly s 525 O31u As p As p vb t O31u 605 I Ie Ar g Pr o 495 Ar g Le u Ph e Ph e ValI 575 Tr p Ar g Le u ValI 480 Tyr Al a G31y G31y As n 560 Ser Hi s Ser <210O> 111 <21 1> 1284 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (1261) <223> RXN00116 <400> 111 cgcggcacgc acgct ggggg caagcgt cga caagcacaaa t t tgcgcacc aat caat ggg ggat caaat a t agt agct gc gt c gt t t ct agg ct t aga ccc t tt ggt Val Val Ser Ar g Leu Arg Pro Phe G1y acc cag cga gct gtt gag gcg ggt gca Thr O31n Ar g Al a Val O31u Al a G31y Al a 30 cct gat gag gat ggt cct cgt cgg at g Pr o Asp O31u Asp O31y Pro Arg Arg I'b t 45 gaa acg O3 u Thr 15 at c aat I I e As n tta gag Leu 03 u Page ct tt t tgct t aat tgaat cc at g agt aat gac tt c I'b t Ser Asn Asp Phe 1 at t t tt gca acc at g I I e Phe Al a Thr I'b t ct t ggt cag ggc tt t Leu 03 y 03 n 03 y Phe at c gcg t cg gag cag Il e Al a Ser 03 u 03 n seql i st cor r ect ed. t xt att ctc ggg gga aat aat cag tat tcg gcg ggg cgt Ile Leu Gy y Asn Asn n Tyr Ser Ala y Arg 60 ggg gat gct t cg G y Asp Al a Ser ttg Leu t ac Tyr at t SIle gtt Val ggg Gy gat Asp 150 at g let aag Lys ttg Leu aag Lys acg Thr 230 ggg Gy aag Lys gcg Al a ggg Gy ggg Gy 310 gca Al a cct Pr o gcg Al a gaa G u 120 acg Thr gat Asp at c SIle gcg Al a ttg Leu 200 gt g Val tcg Ser gcg Al a ttt Phe gcg Al a 280 gag G u aag Lys gt g Val tcg Ser gt g Val t at Tyr gt g Val gat Asp aat Asn 170 aag Lys gat Asp gt c Val gcg Al a gca Al a 250 tct Ser gaa G u aag Lys cat Hi s gct Al a 75 gag G u tt g Leu tac Tyr gcg Al a aag Lys 155 tcg Ser cag G n gag G u gcg Al a gcg Al a 235 ccg Pr o t at Tyr cat Hi s cgg Ar g gac Asp 315 cgt Ar g gt g Val ggt Gy gat Asp gtt Val 140 ttg Leu ccg Pr o ttg Leu gt g Val aag Lys 220 aaa Lys gag G u gt g Val gag G u gat Asp 300 agt Ser cat Hi s at c SIle gt g Val 110 t at Tyr ttg Leu gcg Al a aat Asn ggt Gy 190 gag G u ccc Pr o ttc Phe ttg Leu gct Al a 270 aag Lys ttg Leu ggc Gy ttg Leu acg Thr 95 gag G u gcg Al a cag G n gcg Al a ccg Pr o 175 gtt Val cat Hi s ggt Gy aat Asn ttg Leu 255 aca Thr tgg Tr p cgt Ar g acg Thr gag G u gt g Val cct Pr o gcg Al a gag G u gt g Val 160 acg Thr gct Al a ctt Leu at g Met gt g Val 240 gag G u cct Pr o gt g Val act Thr t at Tyr 320 agg Ar g ggg Gy ggg Gy gct Al a gt g Val 145 act Thr ggt Gy cgt Ar g gtt Val tgg Tr p 225 act Thr gcg Al a ttt Phe t ca Ser gcg Al a 305 ttc Phe ttt Phe gcc Al a gat Asp at t l Ie 130 gag G u aag Lys tcg Ser gcg Al a ttt Phe 210 gat Asp ggt Sy gt g Val cag G n aag Lys 290 tta Leu at c SIle gat Asp act Thr gaa G u 115 gcg Al a aac Asn aag Lys gt g Val t at Tyr 195 gat Asp cgc Ar g tgg Tr p ttg Leu ccg Pr o 275 at g Met gat Asp gtt Val ct g Leu gag G u 100 gt g Val ttg Leu tcg Ser acg Thr ttt Phe 180 gac Asp gat Asp acg Thr aag Lys aag Lys 260 gct Al a tct Ser aag Lys gcg Al a 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 155 att ggg gat cgt gat ggt gcg gag t I e G y Asp Arg Asp G y Al a G u F 330 gtt ggg gtg gcg gcg att ccg gtg c Val G y Val Al a Al a Ile Pro Val C 345 3 aag tgg tcg tcg aag gtt cgt ttt g Lys Trp Ser Ser Lys Val Arg Phe A 360 365 ctc cgc gaa gct gcg gag cgt ctc a Leu Arg G u Ala Ala G u Arg Leu L 375 380 tagtttgaac aggttgttgg ggg <210> 112 <211> 387 <212> PRT <213> Corynebacter i um gl ut am cum Si st cor r ect ed. t xt tc tgt ttt gag ttg att gag aag 'he Cys Phe G u Leu I e G u Lys 335 340 ag gcg ttt gtg gat cat ccg aag Sn Ala Phe Val Asp His Pro Lys 50 355 icg ttt tgc aaa aaa gaa gag acg la Phe Cys Lys Lys G u G u Thr 370 .ag ggg att aag aaa cta ys G y Ile Lys Lys Leu 385 1123 1171 1219 1261 1284 <400> 112 Mvt Ser A 1 Ile Phe A Leu G y G I e Al a S Arg G y A Arg Phe A G y Al a T Sy Asp G 1 Al a I e A 130 Val u A 145 Thr Lys L G y Ser V Ar g Al a T 1 Val Phe A 210 sn Asp I a Thr In y er G u sp Al a sp Leu hr G u 100 lu Val 15 I a Leu sn Ser ys Thr al Phe 180 yr Asp 95 sp Asp Phe Val 5 bet Thr Phe Pro Gn lie Ser Leu 70 G u Tyr Ala I e Ile Val Al a G y Trp Asp 150 Arg IVet 165 Ser Lys Leu Leu G n Lys Val Ser Arg Leu 10 G n Arg Ala Val 25 Asp u Asp y 40 Leu G y G y Asn 55 Arg Ala Ala Val Asn Pro Asp Ser 90 Thr Al a Thr Val 105 Leu G u Pro Tyr 120 Ala Thr Arg Val 135 Val Asp Val Asp iIe Ile Val Asn 170 Lys Ala Leu Lys 185 Val Leu Ser Asp 200 His Val Ser Val 215 Ar g G u Pr o Asn Al a 75 G u Leu Tyr Al a Lys 155 Ser a n G u Al a Pr o Al a Ar g G n Ar g Val Sy Asp Val 140 Leu Pr o Leu Val Lys 220 Phe Sy Ar g Tyr Asp Leu Leu Al a 125 Pr o Hi s Hi s Al a Tyr 205 Leu Gy Al a Met Ser Hi s SIle Val 110 Tyr Leu Al a Asn Sy 190 G u Pr o G u l e Leu Al a Leu Thr G u Al a G n Al a Pr o 175 Val Hi s Sy Thr Asn G u Sy G u Val Pr o Al a G u Val 160 Thr Al a Leu Met Trp Asp Arg Thr Val Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val Page 156 seql i st cor r ec 225 230 235 Thr Gy Trp Lys Thr G y Trp Ala Leu Ala Pro 245 250 Al a Val Leu Lys Al a Lys G n Phe Met Ser Tyr 260 265 Phe G n Pro Ala Val Al a His Ala lle G u His 275 280 Ser Lys IVet Ser Lys G y Leu G u Leu Lys Arg 290 295 Ala Leu Asp Lys Ala G y Leu Lys Thr His Asp 305 310 315 Phe Ile Val Ala Asp Ile G y Asp Arg Asp y 325 330 Su Leu Ile u Lys Val y Val Ala Ala Ile 340 345 Val Asp His Pro Lys Lys Trp Ser Ser Lys Val 355 360 Lys Lys G u G u Thr Leu Arg G u Ala Ala G u 370 375 Lys Lys Leu 385 <210> 113 <211> 607 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(607) <223> FRXA00116 <400> 113 tttgcgcacc aatcaatggg ggatcaaata tagtagctgc ttctaggctt agaccctttg gtgaaacgat ttttgcaacc ted. txt G u Pro Val G y G u G n 285 Asp II e 300 Ser Mbet Al a G u Pro Val Arg Phe 365 Arg Leu 380 Leu Al a 270 Lys Leu Gy Phe G n 350 Al a Lys Leu 255 Thr Tr p Ar g Thr Cys 335 Al a Phe Gy atgagtaatg act t cgtcgt atg acc cag cga gct gtt Val ggt Gy aat Asn gt g Val tcg Ser gcg Al a cgt Ar g cag G n cgt Ar g gt g Val gca Al a at g Met tcg Ser cat Hi s at c SIle at c l Ie tta Leu gcg Al a tt g Leu acg Thr aat Asn gag G u ggg Gy gag G u gt g Val ggt Gy gcg Al a ggg Gy ttt Phe gcc Al a bet Thr G n Arg Ala 1 cag ggc ttt cct gat gag gat Sn y Phe Pro Asp u Asp 15 tcg gag cag att ctc ggg gga Ser G u G n I e Leu G y G y gat gct tcg ttg agg gca gct Asp Ala Ser Leu Arg Ala Ala gat ctg gag tac aac cct gat Asp Leu G u Tyr Asn Pro Asp act gag gcg att acg gcg act Thr G u Ala I e Thr Ala Thr Page 157 gt g ValI t at Tyr gt g ValI g at As p aat As n 1 50 aag Ly s ggt G31y g at As p gt t ValI 120 tt g Le u ccg Pr o tt g Le u gt g ValI 90 t at Tyr tt g Le u gcg Al a aat As n 75 gag O31u gcg Al a cag O31n gcg Al a ccg Pr o 155 cct Pr o gcg Al a gag O31u gt g ValI 140 ac g Thr seql i st car r ect ed. t xt ggg gat gaa gt g at c gt t G31y As p O31u Val I Ie Val 95 gct at t gcg t tg gcg ggg Al a I I e Al a Leu Al a 03 y 110 gt g gag aac t cg t gg gat Val O31u As n Ser Tr p As p 125 130 act aag aag acg cgg at g Thr Lys Lys Thr Ar g l'b t 145 ggt t cg gt g t tt t ct aag 03 y Ser Val Phe Ser Lys 1 tt g Le u gcg Al a 115 gt g ValI at t I Ie aag Ly s g aa (31u 1 00 ac g Th r g at As p at c I Ie gcg Al a <210> 114 <211> 169 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 114 l'bt Thr 03 n Arg AlaVal Ou Ala(3y Ala HeAsn Leu OyOn Oy Ph e O31n Ser O31u Al a I Ie Al a Tr p Ar g 145 Ser As p Le u Ar g As n Thr Le u Al a 115 ValI I Ie Ly s As p G31y Al a As p Thr Pr o Ar g ValI ValI Le u 1 Pr a As n Al a O31u Le u Tyr Al a Ly s 135 Ser O31n Ar g Tyr As p Le u Le u Al a 1 05 Pr a Hi s Hi s Al a 10 vb t Ser Hi s I Ie ValI 90 Tyr Le u Al a As n Le u Al a Le u Thr (31u Al a O31n Al a Pr a 1 I Ie Ar g Ar g O31y O31y Al a ValI 125 Thr (31y Al a O31y Ph e Al a As p I Ie 110 O31u Ly s Ser Ser As p As p Thr O31u Al a As n Ly s ValI <210> 115 Page 158 seql i st cor r ect ed. t xt <211> 1230 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1207) <223> RXN00618 <400> 115 gctgcat t ag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cga Ar g gct Al a gag Gu gat Asp gac Asp ggt Gy gt g Val tct Ser ttc Phe gct Al a 150 ccg Pr o gtt Val ct g Leu cac Hi s cag G n gct Al a gag G u gac Asp ttc Phe at g Met ggg Gy 120 cca Pr o at t l Ie gag Gu at c SIle act Thr 200 cgc Ar g t ca Ser cgc Ar g cgt Ar g aac Asn gca Al a acc Thr aag Lys gct Al a acc Thr gag Gu 170 gat Asp cat Hi s cgc Ar g ggt Gy ggt Gy cgc Ar g gac Asp ttt Phe ggg Gy ct g Leu at g Met 140 cca Pr o at c SIle gac Asp ttt Phe gaa Gu gcg Al a cct Pr o 45 at c SIle aat Asn at c SIle t ac Tyr aac Asn 125 ttg Leu gga Gy gcc Al a t ac Tyr t cc Ser 205 ggc Gy cca Pr o ttg Leu gcc Al a gtt Val gcc Al a ccg Pr o 110 ct g Leu gag Gu aac Asn aag Lys cac Hi s 190 aag Lys aaa Lys 15 gaa Gu gga Gy gat Asp at t SIle acc Thr 95 gca Al a cgc Ar g gaa Gu cca Pr o tgg Tr p 175 ggc Gy aac Asn gac Asp gca Al a t ac Tyr tgg Tr p gt c Val 80 ttg Leu t at Tyr tgt Cys ct g Leu acg Thr 160 tgc Cys at g Met gcc Al a cagataggct tgtctct t at atg cag atg ttg gac 11! IVet G n IVet Leu Asp 1 acc tta atg ttc tgc 16 Thr Leu IVet Phe Cys gtc atc gaa gaa gca 21 Val Ile Gu Gu Ala acc gag gtg att ggt Thr G u Val Ile G y cac tct gct act tat His Ser Ala Thr Tyr acc acc ggt tct tca Thr Thr G y Ser Ser gat cac ggg gat tat Asp Hi s G y Asp Tyr 100 cgc aat att ctg gaa Arg Asn lie Leu u 115 act gca gag act cgt 49! Thr Ala G u Thr Arg 130 cca cac aag ccg aag 54 Pro His Lys Pro Lys 145 ggc acc atc att gat 59! Sy Thr Ile Ile Asp 165 gat gac aat gat gct 64; Asp Asp Asn Asp Ala 180 agc ttt ggt cgt ccg 69 Ser Phe Gy Arg Pro 195 atc gtg gtg ggt acc 73! lie Val Val Gy Thr 210 Page 159 ttg tcc aag tac ttc tcc atg ac Leu Ser Lys Tyr Phe Ser Met Th 215 220 seql i st cor r ect ed. t xt g ggt tgg cgc gtg gg r G y Trp Arg Val G t tgg atc atc y Trp Ile Ile gt t cca gat Val Pro Asp 230 tcc ttg tgt Ser Leu Cys act ttg gag Thr Leu G u gag gcc cgg G u Al a Ar g 280 act ttc gcc Thr Phe Al a 295 gca t ac acc Al a Tyr Thr 310 gcg ggc gtg Al a y Val cac aag tgg His Lys Tr p gaa ggt gt g G u G y Val 360 ct g Leu cct Pr o 250 ggg Sy gt g Val ccg Pr o gat Asp gt c Val 330 cgt Ar g gt c Val 235 gcc Al a gcc Al a tt c Phe gat Asp t ca Ser 315 gcg Al a tt g Leu aca Thr at c l Ie gaa G u gt c Val ggc G y 300 gag Gu ccg Pr o agc Ser ccg Pr o ggg G y ctt Leu gat Asp 285 ggc G y gaa Gu ggt Sy ct g Leu gaa G u 365 at t SIle cag G n gat Asp 270 aag Lys ct g Leu tgg Tr p gtt Val tgc Cys 350 gaa G u gct Al a 255 gcc Al a ct c Leu t at Tyr gca Al a gat Asp 335 gcg Al a aac Asn 240 gcg Al a cac Hi s cct Pr o ttg Leu ttg Leu 320 ttt Phe t ca Ser 225 ct g Leu gga G y gtt Val gaa G u tgg Tr p 305 cgt Ar g gat Asp aag Lys cag G n cgc Ar g gaa G u at c SIle 290 gt c Val ttg Leu cct Pr o gaa G u gct Al a gca Al a gcg Al a 275 ggg G y gat Asp ct c Leu gag G u gac Asp 355 tct Ser gcc Al a 260 t at Tyr ctt Leu gtt Val gat Asp gaa G u 340 acc Thr ctt Leu 245 ttc Phe cgc Ar g ggc G y t ct Ser gaa Gu 325 ggc Sy at t SIe 787 835 883 931 979 1027 1075 1123 1171 1217 1230 aaa at c gga Lys I I e y ttc atc aaa aaa Phe Ile Lys Lys tagcagcgac taggttagtt tcg <210> 116 <211> 369 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 116 IVet G n Iet Leu Asp Arg Val His Arg 1 5 Thr Leu IVet Phe Cys Al a Gy n Pro 25 Val Il e u u Ala u Ile Ala Leu 40 Thr u Val I e y Asp Arg u Phe His Ser Ala Thr Tyr Asp Val Asp Thr 70 Thr Thr G y Ser Ser G y G y Phe Val Asp His G y Asp Tyr Val Ala IVet Pro Arg Arg 10 Ser Thr Arg Ser Arg Gu Asn Pro 75 Al a Ser I 90 Thr Pro Page 160 G u Al a Pr o I le Asn SIle Tyr Sy Pr o Leu Al a Val Al a Pr o Lys G u Sy Asp SIle Thr Al a Asp Al a Tyr Tr p Val Leu Tyr seql i st cor r ect ed. t xt 100 Leu G u Ser Leu 105 Al a Lys Val Ar g Thr Pr o 145 Gy Asp Ser SIle Val 225 Leu Gy Val G u Tr p 305 Ar g Asp Lys Lys Asn Il e 115 Al a G u 130 Hi s Lys Thr Ile Asp Asn Phe G y 195 Val Val 210 Sy Trp G n Al a Arg Ala G u Al a 275 ie G y 290 Val Asp Leu Leu Pro G u Su Asp 355 Ar g Lys Asp 165 Al a Pr o Thr SIle Leu 245 Phe Ar g Sy Ser G u 325 G y lie Phe Al a 150 Pr o Val Leu Leu Val 230 Ser Thr G u Thr Al a 310 Al a Hi s G u Q n 135 Val G u Leu Al a Ser 215 Pr o Leu Leu Al a Phe 295 Tyr Sy Lys Sy Sy 120 Pr o SIle Su I e Thr 200 Lys Asp Cys G u Ar g 280 Al a Thr Val Tr p Val 360 Thr Val Leu Ser 185 Al a Tyr G u Al a Al a 265 a u Asp Asp Al a SIle 345 Ar g Al a Thr G u 170 Asp Hi s Phe Leu Pr o 250 Sy Val Pr o Asp Val 330 Ar g Lys O n Ser 155 Ar g G u G n Ser Val 235 Al a Al a Phe Asp Ser 315 Al a Leu SIle Leu Asn 125 Met Leu 140 Pro G y I e Al a Asp Tyr Phe Ser 205 lMt Thr 220 Thr Pro I e G y G u Leu Val Asp 285 G y G y 300 G u G u Pro G y Ser Leu 3y u 365 Leu Arg G u G u Asn Pro Lys Trp 175 Hi s G y 190 Lys Asn Sy Trp Ile u G n Al a 255 Asp Al a 270 Lys Leu Leu Tyr Trp Al a Val Asp 335 Cys Ala 350 Phe l e <210> 117 <211> 657 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(634) <223> FRXA00618 <400> 117 cccaacgggc accatcattg atccggaaga gctagagcgc atcgccaagt ggtgcgatga caatgatgct gttcttatct ctgatgagga ctaccacggc atg agc ttt ggt cgt 115 Page 161 seqI i st car r ect ed. t xt I'bt Ser Phe G31y Ar g ccg Pr o ac c Thr at c I Ie ct t Le u tt c Ph e cgc Ar g ggC G31y t ct Ser g aa O31u G31y 1 50 at t I Ie gca Al a t cc Ser cca Pr o tt g Le u tt g Le u gcc Al a tt c Ph e t ac Tyr 120 ggC G31y aag Ly s ggt G31y gcg Al a t ac Tyr gag O31u gct Al a gct Al a gag O31u g ac As p g at As p gcc Al a at t I Ie cgc Ar g 1 70 C ag O31n t cc Ser gt c ValI gcc Al a gcc Al a tt c Ph e g at As p t ca Ser gcg Al a 140 tt g Le u tt t Ph e at g vb t ac a Thr 45 at c I Ie g aa O31u gt c ValI G31y gag O31u 125 ccg Pr o ag C Ser t cc Ser ac g Thr ccg Pr o ggg G31y ct t Le u g at As p ggC G31y 110 g aa (31u ggt G31y ct g Le u aac As n t gg Tr p g aa (31u gct Al a gcc Al a 80 ct c Le u t at Tyr gca Al a g at As p gcg Al a 1 60 gcc Al a cgc Ar g aac As n gcg Al a C ac Hi s cct Pr a tt g Le u tt g Le u tt t Ph e 145 t ca Ser at c I Ie gt g ValI ct g Le u gga G31y gt t ValI g aa (31u t gg Tr p cgt Ar g 130 g at As p aag Ly s gt g ValI ggt G31y C ag O31n cgc Ar g g aa O31u at c I Ie gt c ValI 115 tt g Le u cct Pr a g aa O31u gt g ValI t gg Tr p gct Al a gca Al a gcg Al a ggg G31y 1 00 g at As p ct c Le u gag O31u g ac As p ggt G31y at c I Ie t ct Ser gcc Al a t at Tyr ct t Le u gt t ValI g at As p g aa (31u ac C Th r 1 aaa at c gga gaa t tc at c aaa aaa Lys Ii e 0 y 0u Phe Il e Lys Lys 1 t agcagcgac t aggt tagt t t cg <210O> 118 <21 1> 178 <21 2> PRT <21 3> Cor ynebact er i urn gi <400> 118 I'bt Ser Phe G31y Ar g Pro 1 5 Ala I Ie Val Val G31y Th r Ar g Val G31y Tr p I Ie I Ie Asn Leu 03 n Al a Ser Leu ut ani cumn Leu Al a Thr Al a Hi s O31n Phe Ser Lys Asn 10 Leu Ser Lys Tyr Phe Ser I'b t Thr G31y Tr p 25 Val Pr o As p O31u Leu Val Thr Pr o I Ie O31u 40 Ser Leu Cys Al a Pro Al a I Ie 31y O31n Al a 55 Page 162 Al a Hi s Pr o Leu Leu Phe 145 Ser Lys Ar g G u SIle Val 115 Leu Pr o G u Al a Tyr Leu Val Asp G u Thr 165 Thr G u Thr Al a Al a 135 Hi s G u seql i st cor r ect ed. t xt Leu G u Al a y Al a G u Ala Arg G u Val Phe Val 90 Phe Ala Asp Pro Asp G y 105 Tyr Thr Asp Asp Ser G u 120 125 Sy Val Ala Val Ala Pro 140 Lys Trp lie Arg Leu Ser 155 Sy Val Arg Lys Ile y 170 Asp Lys Leu Tr p Val Cys Phe 175 <210> 119 <211> 385 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(385) <223> FRXA00627 <400> 119 gctgcat t ag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cga Ar g gct Al a gag G u gat Asp gac Asp ggt Sy cac Hi s cag G n gct Al a gag G u gac Asp ttc Phe cgc Ar g t ca Ser cgc Ar g cgt Ar g aac Asn gca Al a cgc Ar g ggt Sy ggt Sy cgc Ar g gac Asp ttt Phe gaa G u gcg Al a cct Pr o 45 at c SIle aat Asn at c SIle ggc G y cca Pr o ttg Leu gcc Al a gtt Val gcc Al a aaa Lys 15 gaa G u gga Sy gat Asp at t SIle acc Thr gac Asp gca Al a t ac Tyr tgg Tr p gt c Val 80 cagataggct tgtctct t at atg cag atg ttg gac IVet G n IVet Leu Asp 1 acc tta atg ttc tgc Thr Leu IVet Phe Cys gtc atc gaa gaa gca Val Ile u u Ala acc gag gtg att ggt Thr u Val Ile y cac t ct gct act tat His Ser Ala Thr Tyr acc acc ggt tct tca Thr Thr G y Ser Ser <210> 120 Page 163 seqi i st car r ect ed. t xt <21 1> <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 120 Nbt (31n N/t Leu Asp Ar g Val Hi s Ar g 1 5 Thr Leu N'bt Phe Cys Ala O1y On Pro 25 Val I Ie (1u (1u Aia (1u I IeAia Le u 40 Thr O31u Val I IIe O31y Asp Ar g O31u Ph e Hi s Ser A a Thr Tyr Asp Val Asp Thr 70 Thr Thr 03 y Ser Ser 03 y 03 y Phe Val Ar g Thr Ser (31u Pr o 75 Ser G31u A a Pr o li1e As n I Ie G31y Pr o Le u A a ValI A a Lys5 (31u G31y As p I Ie Thr As p A a Tyr Tr p ValI <210O> 121 <21 1> 1434 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 ODS <222> (1411) <223> RXA02550 <400> 121 gat ct taggc agccgt ggga t tacaccct t t tagagct ag t agct t tcaa ct acgcacac aaagt ggcaa cat tgagcgg aacagtaaaa attcacccaa cgc Ar g g at As p c ag O31n gcc A a ct c Le u at t I Ie ac c Thr ac c Thr gca A a gag O31u gag O31u ac g Thr cgt Ar g aaa Ly s aag Ly s cgt Ar g at g vb t g aa O31u aat As n at g vb t at t I Ie acc Thr ccc Pr o aag Ly s cgc Ar g cca Pr a 75 at c I Ie at t I Ie ac c Thr act Thr g ac As p at g vb t gcc A a gcc A a ccg Pr a ac c Thr cga Ar g ct g Le u ct t Le u tt c Ph e ct t Le u cgg Ar g 110 gcc A a 15 ac a Thr t ac Tyr g at As p gga G31y cca Pr a 95 cga Ar g aac As n act Thr gag O31u ggg G31y tt c Ph e act Thr gca A a act Thr gct A a cgc Ar g cgt Ar g aac As n gcc A a c aa O31n gt c ValI ac a Thr gt g ValI at c I Ie ggc G31y at c I Ie ccc Pr a ggg G31y ac c Thr 115 g ac As p ggc G31y tt c Ph e ccg Pr a tt a Le u g ac As p t at Ty r 1 00 cgc Ar g Page 164 seql i st cor r ect ed. t xt gaa G u ggc G y aac Asn 150 act Thr gat Asp at c SIle acg Thr gca Al a 230 at t SIle gat Asp gca Al a gca Al a tgc Cys 310 cgc Ar g cag G n tgt Cys aac Asn gt g Val 120 ggt Sy ggc G y gca Al a gaa G u gag Su 200 gct Al a gag G u t ac Tyr ctt Leu t ac Tyr 280 gga G y aat Asn t cc Ser aac Asn aaa Lys 360 t ac Tyr ccc gga Pro G y gtc t ca Val Ser gat gaa Asp u acc t cc Thr Ser 170 gat gac Asp Asp 185 aaa acc Lys Thr gtc tac Val Tyr cat gac His Asp gat gat Asp Asp 250 tgc at c Cys I I e 265 cga gct Arg Ala ttt att Phe Il e gtc cca Val Pr o atc tac Ile Tyr 330 atg gca vet Ala 345 cca at g Pro 'bet gaa at c G u lie ttc ccc cac Phe Pro Hi s 125 gaa ct a at c G u Leu Il e 140 gtt ctt atc Val Leu Ile 155 ct g gct ggt Leu Ala G y tgg aac cca Trp Asn Pr o aaa gct att Lys Ala lle 205 ccg cgc cgg Pro Arg Ar g 220 ctg ctg att Leu Leu Il e 235 gcc gag cac Al a G u Hi s aca tac aac Thr Tyr Asn ggc tgg at g G y Trp IVet 285 gag ggc ct c G u G y Leu 300 gct cag cac Al a G n Hi s 315 gac ct c act Asp Leu Thr tgg acg aaa Trp Thr Lys gga gct ct a G y Al a Leu 365 cac gac gac Hi s Asp Asp 380 ttc gat gtt gat gat Phe Asp Val Asp Asp 130 acc atg acc acc caa Thr Met Thr Thr G n 145 ccc gca ccg gac tac Pro Ala Pro Asp Tyr 160 ggt aag cct gtg cac Gy Lys Pro Val Hi s 175 t cc at c gaa gac at c Ser lie G u Asp lie 190 gtg gtg atc aac ccc Val Val lie Asn Pro 210 gtg ttg gaa caa atc Val Leu u n lie 225 ttg gcc gat gaa atc Leu Ala Asp G u Ile 240 atc agc ctg gca acc Ile Ser Leu Ala Thr 255 ggt cta tcc aag gca Sy Leu Ser Lys Ala 270 gta ttg act gga cca Val Leu Thr Gy Pro 290 gaa ctc ctc gca ggc G u Leu Leu Ala G y 305 gct att cag gta gct Ala lle n Val Ala 320 ggc gaa cac ggc cga Gy u His y Arg 335 ctc aac gaa atc cca Leu Asn u lie Pro 350 tac gcg ttc ccc aag Tyr Ala Phe Pro Lys 370 acc caa ctc atg ctg Thr G n Leu Iet Leu gt g Val gca Al a cca Pr o t ac Tyr aag Lys 195 aac Asn gt c Val t ac Tyr ctt Leu t ac Tyr 275 aag Lys act Thr ct g Leu ct c Leu ggt G y 355 ct c Leu ttc Phe ct c Leu ct g Leu ct c Leu 180 t cc Ser aac Asn gag G u gac Asp gca Al a 260 cgc Ar g caa G n cga Ar g ggt G y ct g Leu 340 gt c Val gac Asp 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 gat ctt ctc Asp Leu Leu 385 Page 165 seql i st cor r ect ed. t xt cgt gcc gag aaa atc ctc atg gtt cag ggc act ggc ttc aac tgg cca Arg Ala G u Lys Ile Leu IVet Val G n y Thr y Phe Asn Trp Pro 390 395 400 405 cat cac gat cac ttc cga gtg gtc acc ctg cca tgg gca tcc cag ttg His His Asp His Phe Arg Val Val Thr Leu Pro Trp Ala Ser G n Leu 410 415 420 gaa aac gca att gag cgc ctg ggt aac ttc ctg tcc act tac aag cag G u Asn Ala lie G u Arg Leu y Asn Phe Leu Ser Thr Tyr Lys G n 425 430 435 tagtagttgt taggattcac cac <210> 122 <211> 437 <212> PRT <213> Cor ynebact eri um gl ut am cum 1315 1363 1411 1434 <400> 122 Val Thr Thr Asp Lys Arg Lys Thr 1 Lys Ar g SIle Hi s Asp Ser Val Asp Thr 145 Asp Val Asp Asn G n 225 Val I e Gy SIle Pr o Gy Thr 115 Val Al a Pr o Tyr Lys 195 Asn Val 5 Al a Asp Val Lys Val Ser Tyr Leu Leu Tr p 165 Cys Lys Pr o SIle G n Ser Al a Asn bet Ser Val Asn 135 Asp Al a G u Ser Gy 215 Ar g Al a G u 40 G u Thr Ar g Lys Val 120 Gy Gy Al a G u G u 200 Al a G u Ser Lys 10 Al a Ar g Lys IVet Al a G u Sy Asn Asp IVet 90 G y lle 105 Pro G y Val Ser Asp u Thr Ser 170 Asp Asp 185 Lys Thr Val Tyr His Asp Thr Pr o Lys Ar g Pr o I e SIle Phe G u Val 155 Leu Tr p Lys Pr o Leu 235 Thr Thr Asp Met Al a Al a Pr o Pr o Leu 140 Leu Al a Asn Al a Ar g 220 Leu Asp Ar g Val G u Val Asn Al a Hi s 125 I e I e Gy Pr o l Ie 205 Ar g SIle Al a Thr Tyr Asp Gy Pr o Ar g Asp bet Al a Lys 175 I e Val Leu Al a Ou Ile Tyr Asp Arg lle Leu Tyr Asp Asp Ala u His Ile Ser Leu Page 166 seql i st cor r ect ed. t xt 245 Al a Lys Gy Al a 305 Val Gy SIle Pr o Met 385 Gy Tr p Ser Leu Ala Pro 260 Tyr Arg Val 275 Lys G n Tyr Thr Arg Leu Leu G y G y 325 Leu Leu G u 340 G y Val Ser 355 Leu Asp Pro Asp Leu Leu Asn Trp Pro 405 Ser G n Leu 420 Tyr Lys G n 435 Asp Leu Leu Al a Al a Cys 310 Ar g G n Cys Asn Ar g 390 Hi s G u Gy Ar g 295 Pr o G n Ar g Val Val 375 Al a Hi s 250 Cys II e Thr 265 Ar g Al a G y Phe II e G u Val Pr o Al a 315 Ile Tyr Asp 330 IVet Al a Tr p 345 Pro e t G y G u Il e Hi s Lys IIe Leu 395 Hi s Phe Ar g 410 Tyr Asn G y 270 Trp NMt Val 285 G y Leu u 300 G n His Al a Leu Thr G y Thr Lys Leu 350 Ala Leu Tyr 365 Asp Asp Thr 380 Mvt Val G n Val Val Thr 255 Leu Ser Leu Thr Leu Leu lie n 320 G u Hi s 335 Asn u Al a Phe G n Leu G y Thr 400 Leu Pro 415 Asn Ala lie Gu Arg Leu y 425 Asn Phe Leu 430 <210> 123 <211> 1701 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1678) <223> RXA02193 <400> 123 cccccgaaaa tgtagtgggt agt t ccacac tgcctct t ac aagtacgtag gataatccac agcaccattg tgatttcctt caacttgtga gaggcagtac atg tct aag acg agc IVet Ser Lys Thr Ser 1 aac aag tct tca gca gac tca aag aat gac gca aaa gcc gaa gac att Asn Lys Ser Ser Ala Asp Ser Lys Asn Asp Ala Lys Ala G u Asp Ile 15 gtg aac ggc gag aac caa atc gcc acg aat gag tcg cag tct tca gac Val Asn y G u Asn G n I e Al a Thr Asn u Ser G n Ser Ser Asp 30 agc gct gca gtt tcg gaa cgt gtc gtc gaa cca aaa acc acg gtt cag Ser Ala Ala Val Ser G u Arg Val Val G u Pro Lys Thr Thr Val G n 45 Page 167 aaa aag Lys Lys t cc cac Ser His caa at c Gn Ilie atg gt c MVt Val aca ctt Thr Leu at c ct c I e Leu 135 cag ggt G n Gy 150 gcc aac Al a Asn at c ct g I e Leu t cc tac Ser Tyr ct c at c Leu II e 215 aat gag Asn u 230 gct gtt Al a Val ctc gca Leu Al a gag gtc G u Val gca ggc Al a G y 295 ctg gaa Leu G u 310 ttc cga atc gaa tcg ga Phe Arg Ile u Ser As seql i st cor r ect ed. t t ctg ctt ggt gaa p Leu Leu G y G u ctt cag atc cca Leu Gn Ile Pro gca Al a t ca Ser cag G n cca Pr o 120 at t SIle ggc Gy ctt Leu cac Hi s cca Pr o 200 gct Al a ttt Phe ccc Pr o gaa G u aat Asn 280 t ac Tyr ct a Leu t ac Ty r acc Thr aaa Lys caa G n gaa G u ggt Gy ctt Leu 170 at g Met ggt Sy at t l Ie gac Asp agc Ser 250 cag G n ggt Sy cac Hi s t cc Ser ggg Gy 75 acc Thr aag Lys aaa Lys cgc Ar g acc Thr 155 gag G u gat Asp tt c Phe gat Asp at c SIle 235 tt g Leu acc Thr gca Al a cag G n gca Al a 315 gt g Val at c SIle gcc Al a gca Al a tgt Cys 140 t ca Ser ttc Phe gat Asp cgc Ar g gag G u 220 at c SIle ggc G y gt g Val acc Thr gtt Val 300 cgt Ar g cac Hi s aac Asn gca Al a gaa G u 125 at g Met ct g Leu tta Leu gt g Val ct g Leu 205 ctt Leu aag Lys gaa G u ct g Leu gca Al a 285 gt c Val gat Asp acc Thr cac Hi s gct Al a 110 gca Al a gat Asp aac Asn ggc Sy aac Asn 190 ggc Sy cag G n at g Met gag G u cgt Ar g 270 at c l Ie gct Al a ct c Leu ctt Leu gt c Val 95 tta Leu at t l Ie cag G n at g Met cat Hi s 175 at g Met at t SIle gtt Val ggc Sy ttc Phe 255 gaa G u ggt G y gct Al a at c SIle cgt Ar g cca Pr o gca Al a gt c Val ttc Phe aac Asn 160 gaa G u t cc Ser t ac Tyr gcg Al a cgc Ar g 240 cga Ar g gct Al a act Thr ct g Leu gag 30u 320 gcg Al a gat Asp aac Asn tgg Tr p ccc Pr o 145 acc Thr aag Lys cag G n gct Al a ttc Phe 225 acc Thr gca Al a gcc Al a ggt G y tct Ser 305 gct Al a gt g Val ttc Phe cgc Ar g gct Al a 130 at c SIle aac Asn ggc Sy t cc Ser gga Sy 210 cgc Ar g cag G n ttc Phe aac Asn gt g Val 290 gag G u acc Thr aac Asn cgc Ar g 100 ct g Leu gat Asp gt g Val gtt Val t ac Tyr 180 aac Asn cag G n aag Lys cag G n cac Hi s 260 ct c Leu act Thr acc Thr gac Asp 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 168 seql i st cor r ect ed. t xt ggt gca tat gtt cat gcg cac tcc gca atc aag cgt gca gcc atg aaa 1123 Sy Ala Tyr Val His Ala His Ser Ala lie Lys Arg Ala Ala Met Lys 330 335 340 ctg tcc aag atc tgt aac gat cta cgt ctg ctg tct tct ggt cct cgt 1171 Leu Ser Lys lie Cys Asn Asp Leu Arg Leu Leu Ser Ser y Pro Arg 345 350 355 gct ggc ttg aac gaa atc aac ctg cca cca cgc cag gct ggt tcc tcc 1219 Ala y Leu Asn G u Ile Asn Leu Pro Pro Arg G n Ala y Ser Ser 360 365 370 atc atg cca gcc aag gtc aac cca gtg atc cca gaa gtg gtc aac cag 1267 Ile IVet Pro Ala Lys Val Asn Pro Val Ile Pro G u Val Val Asn G n 375 380 385 gtc tgc ttc aag gtc ttc ggt aac gat ctc acc gtc acc atg gct gcg 1315 Val Cys Phe Lys Val Phe G y Asn Asp Leu Thr Val Thr IVet Al a Al a 390 395 400 405 gaa gct ggc cag ttg cag ctc aac gtc atg gag cca gtc att ggc gaa 1363 Su Ala Gy On Leu n Leu Asn Val IVet u Pro Val Ile Gy u 410 415 420 tcc ctc ttc cag tca ctg cgc atc ctg ggc aat gca gcc aag act ttg 1411 Ser Leu Phe G n Ser Leu Arg Ile Leu G y Asn Al a Al a Lys Thr Leu 425 430 435 cgt gag aag tgc gtc gta gga atc acc gcc aac gct gat gtt tgc cgt 1459 Arg G u Lys Cys Val Val Gy Ile Thr Ala Asn Ala Asp Val Cys Arg 440 445 450 gct tac gtt gat aac tcc atc ggg att atc act tac ctg aac cca ttc 1507 Ala Tyr Val Asp Asn Ser lie Gy lie lie Thr Tyr Leu Asn Pro Phe 455 460 465 ctg ggc cac gac att gga gat cag atc ggt aag gaa gca gcc gaa act 1555 Leu G y Hi s Asp IIe G y Asp G n lie G y Lys G u Al a Al a G u Thr 470 475 480 485 ggt cga cca gtg cgt gaa ctc atc ctg gaa aag aag ctc atg gat gaa 1603 Sy Arg Pro Val Arg G u Leu Ile Leu G u Lys Lys Leu IVet Asp G u 490 495 500 aag acg ctc gag gca gtc ctg tcc aag gag aac ctc atg cac cca atg 1651 Lys Thr Leu G u Ala Val Leu Ser Lys G u Asn Leu IVet His Pro IVet 505 510 515 ttc cgc gga agg ctc tac ttg gag aac taatccaaga tctcgtctga 1698 Phe Arg y Arg Leu Tyr Leu G u Asn 520 525 tac 1701 <210> 124 <211> 526 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 124 IVet Ser Lys Thr Ser Asn Lys Ser Ser Ala Asp Ser Lys Asn Asp Ala 1 5 10 Lys Ala Gu Asp ile Val Asn G y Gu Asn Gn Ile Ala Thr Asn Gu 25 Ser G n Ser Ser Asp Ser Ala Ala Val Ser u Arg Val Val u Pro Page 169 seqI i st car r ect ed. t xt Phe Arg Ilie Ou Asp Leu Leu 03 y Ly s A a As p As n Tr p Pr o 145 Thr Ly s O31n A a Ph e 225 Thr A a A a G31y Ser 305 A a Ar g Ser O31n O31u 385 Thr (31n As p I Ie Ar g 115 cys As p O31u O31u Thr 1 95 Le u Hi s Le u A a Ar g 275 As n ValI Ser A a G31y 355 G31y ValI O3 n Lys Pr o Ser 70 Phe 03 n 31y Ib t Hi s Thr O31n I Ie Phe 03 n 150 Val Ala 1 65 Hi s I I e Asp Ser Thr Leu 31y As n 230 As p A a 245 As n Leu Leu 03 u Pr o Ala O3 y Leu 310 Thr 03 y 325 Lys Leu Ar g Ala Ser I Ie O3 n Val 390 Ly s Hi s I Ie ValI Le u Le u 135 G31y As n Le u Tyr I Ie 215 O31u ValI A a ValI G31y 295 O31u A a Ser G31y vb t 375 Cys A a Ser O31n Pr o 120 li1e O31y Le u Hi s Pr o 200 A a Ph e Pr o O31u As n 280 Tyr Le u Tyr Ly s Le u 360 Pr a Ph e Tyr Ar g ValI 1 05 A a O31u A a A a Pr a 1 85 Thr O31u ValI vb t O31u 265 Le u Ar g Ly s ValI I Ie 345 As n A a Ly s (31y 75 Thr Ly s Ly s Ar g Thr 1 55 O31u As p Ph e As p I Ie 235 Le u Thr A a O31n A a 315 A a As n I Ie ValI Ser ValI I Ie A a A a cys 140 Ser Ph e As p Ar g O31u 220 li1e O31y ValI Thr ValI 300 Ar g Hi s As p As n As n 380 Hi s As n A a O31u 125 vb t Le u Le u ValI Le u 205 Le u Ly s O31u Le u A a 285 ValI As p Ser Le u Le u 365 Pr a Thr Hi s A a 110 A a As p As n O31y As n 1 O31y O31n vb t O31u Ar g 270 li1e A a Le u A a Ar g 350 Pr a ValI Le u ValI Le u I Ie O31n vb t Hi s 1 vb t I Ie ValI O31y Ph e 255 O31u O31y A a I Ie I Ie 335 Le u Pr a I Ie Ar g Pr a A a ValI Ph e As n 1 O31u Ser Tyr A a Ar g 240 Ar g A a Thr Le u O31u 320 Ly s Le u Ar g Pr a Val Phe O31y Asn Asp Leu Thr Page 170 ValI Pr o A a A a Tyr 465 O31u Ly s Le u vb t I Ie Ly s 435 ValI As n A a vb t Hi s 515 A a 405 (31u Le u Ar g Ph e Thr 485 (31u vb t A a Le u (31u Tyr 455 G31y Ar g Th r Ar g seql i st car r ect ed. t xt G3 y G3 n Leu G3 n Leu Asn 410 Phe O31n Ser Leu Arg I Ie 425 Lys Cys Val Val O31y I Ie 440 445 Val As p As n Ser I Ie O31y 460 Hi s As p I I e G3 y As p G3 n 475 Pr o Val Ar g (31u Leu I Ie 490 Leu (31u Ala Val Leu Ser 505 a31y Ar g Leu Ty r Leu (31u 520 525 vb t 415 a31y A a I Ie a31y O31u 495 a u <210O> 125 <21 1> 1098 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1075) <223> RXA02432 <400> 125 cacgt gat tc at t tgt gacc aacaaccgaa act gagccag gt gcacat aa caact gcagc t agt t gat ac gct agagcgc aagact gt ca at cccct gct atg tcg aag cag cac 11! l'bt Ser Lys O31n Hi s t cc Ser gt t ValI aat As n at c I Ie at c I Ie at c I Ie cca Pr o gt a ValI cat Hi s cca Pr o cgc Ar g gcc A a aac As n ac c Thr ct t Le u tt c Ph e g at As p gt t ValI g at As p ggc a31y ac c Thr gga a31y t cc Ser aag Lys5 g aa O31u gga a31y gt c ValI 45 gcg A a t cc Ser gt g ValI g aa O31u ac c Thr ag c Ser c ag O31n at g vb t tt g Le u act Thr gcc A a gca A a gct A a tt t Ph e 80 g at As p gct A a act Thr ct g Le u 50 g aa O31u g at As p g at As p c aa O31n 20 g ac As p gcg A a c ac Hi s g at As p gt t ValI 1 00 aag gca A a cca Pr o g aa O31u t cc Se r ggc a31y gt agt agt t acc cac ggc acc gat t cc at ggaa gag tcc gcc at cgcc Val Val Val Thr Hi s O31y Thr Asp Ser l'bt O31u O31u Ser Ala I Ie Ala Page 171 seql i st cor r ect ed. t xt 105 110 115 gta gac acc ttc ctt gat gat ccc cgc cca gtc att ttc acc ggc gcc 499 Val Asp Thr Phe Leu Asp Asp Pro Arg Pro Val lie Phe Thr y Ala 120 125 130 caa aaa ccc ttc gat cat ccc gaa gcc gac ggc cca aac aac ctt ttc 547 G n Lys Pro Phe Asp His Pro G u Ala Asp G y Pro Asn Asn Leu Phe 135 140 145 gaa gcc tgc ctc atc gca tcc gac ccc tcc gct cgc gga att ggt gca 595 G u Al a Cys Leu lie Al a Ser Asp Pro Ser Al a Arg G y lie G y Al a 150 155 160 165 ctc att gtc ttc ggt cac gcc gtc atc cct gct cgc ggc tgc gtt aaa 643 Leu Ile Val Phe G y His Ala Val I I e Pro Ala Arg y Cys Val Lys 170 175 180 tgg cac acc tct gat gag ctg gcg ttt gca acc aac ggc cct gaa gaa 691 Trp His Thr Ser Asp G u Leu Ala Phe Ala Thr Asn G y Pro G u G u 185 190 195 cca gag cgc ccc gat gcg ctg ccc gta gct aaa ttg gcg gat gtc tct 739 Pro G u Arg Pro Asp Al a Leu Pro Val Ala Lys Leu Ala Asp Val Ser 200 205 210 gtc gaa atc atc ccc gca tac cct ggt gcc acc ggc gca atg gtg gaa 787 Val u Ile Ile Pro Ala Tyr Pro y Ala Thr y Al a Iet Val u 215 220 225 gct gcc atc gct gcc ggt gct caa gga ctt gta gtg gaa gca atg gga 835 Al a Ala Ile Ala Al a y Al a Gn y Leu Val Val u Al a Iet y 230 235 240 245 tca ggc aat gtt ggt tcc cgc atg ggt gat gcc cta ggt aaa gca ctt 883 Ser y Asn Val y Ser Arg IVet y Asp Ala Leu y Lys Ala Leu 250 255 260 gac gct gga att ccc gtg gtg atg agc act agg gtt cct cgt ggt gaa 931 Asp Ala Gy lie Pro Val Val IVet Ser Thr Arg Val Pro Arg Gy G u 265 270 275 gta tcc gga gtg tat ggc ggt gca ggt gga ggt gcg act ttg gct gcg 979 Val Ser y Val Tyr Gy y Ala Gy Gy y Ala Thr Leu Ala Ala 280 285 290 aag ggc gct gtg gga tct cgc tac ttc aga gct ggt cag gca cgt att 1027 Lys y Ala Val y Ser Arg Tyr Phe Arg Ala Gy G n Ala Arg lie 295 300 305 ttg ctc gcg att gcc att gcg acg ggc gca cat ccg gtg acg ctt tac 1075 Leu Leu Ala IIe Ala I e Ala Thr G y Ala His Pro Val Thr Leu Tyr 310 315 320 325 taatttcgcc cttggtcttg cat 1098 <210> 126 <211> 325 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 126 IVet Ser Lys G n His Ser Thr Pro Leu Asn Asn Asp G u G u His Thr 1 5 10 Ser Ala Pro G n Lys Val Ala Val lie Thr Thr Gy Gy Thr I e Ala 25 Page 172 seql i st cor r ect ed. t xt Cys Thr Ser Asp Ala Asn Gy His Leu Leu Pro Thr 40 Val Ser Gy Ala Asp Phe G u Pr o G u SIle Pr o 145 Ar g Ar g Asn Leu Gy 225 Val Leu Val Al a Gy 305 Pr o Leu SIle Leu Val Al a 115 Thr Asn SIle Cys Pr o 195 Asp let Al a Lys Ar g 275 Leu Al a Thr Ala Pro Hi s G u Asp Ser Val G y 100 I e Al a G y Al a Leu Phe G y Al a 165 Val Lys 180 G u G u Val Ser Val G u let G y 245 Al a Leu 260 G y Gu Al a Al a Arg Ile Leu Tyr 325 Al a Asn SIle Val Asp Lys 135 Al a l Ie Hi s G u G u 215 Al a Gy Al a Ser Gy 295 Pr o Ar g Al a Val Thr 120 Pr o Cys Val Thr Ar g 200 I e l e Asn Gy Gy 280 Al a Ar g Leu Thr Thr 105 Phe Phe Leu Phe Ser 185 Pr o SIle Al a Val SIle 265 Val Val Asn Ser 75 Hi s Gy Asp Hi s Al a 155 Hi s G u Al a Al a Gy 235 Ser Val Gy Ser l Ie 315 Gy Ser Lys Thr Asp Pr o 140 Ser Al a Leu Leu Tyr 220 Al a Ar g Val Gy Ar g 300 Al a Ser Val Asp Pr o 125 G u Asp Val Al a Pr o 205 Pr o G n et Met Al a 285 Tyr G n let Leu Ser 110 Ar g Al a Pr o SIle Phe 190 Val Gy Gy Gy Ser 270 Gy Phe SIle Thr G u let Pr o Asp Ser Pr o 175 Al a Al a Al a Leu Asp 255 Thr Gy Ar g Al a Phe Asp G u Val Gy Al a 160 Al a Thr Lys Thr Val 240 Al a Ar g Gy Al a Hi s 320 Leu Ala lie Ala Ala Thr y Al a <210> 127 <211> 775 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(775) <223> RXN03003 Page 173 seql i st cor r ect ed. t xt <400> 127 tcgatgatct gggctcccca ggtggtgcgg ctaagcttgg accacaagat tttgatcacc caatgatcgc tgcgctgccg cctcaggcat aatctaacgc atg acc tct cgc acc 115 ebt Thr Ser Arg Thr 1 ccg ctt gtt tct gtt ctt cct gat ttt ccg tgg gat tcg ctc gct tcc 163 Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp Asp Ser Leu Ala Ser 15 gca aaa gcc aaa gct gcg tct cac ccg gat ggg atc gtg aat ctt tct 211 Ala Lys Ala Lys Ala Al a Ser His Pro Asp G y IIe Val Asn Leu Ser 30 gtt ggc act ccg gtt gat ccg gtc gcg ccc agc att cag atc gcg ttg 259 Val y Thr Pro Val Asp Pro Val Ala Pro Ser lie n lie Ala Leu 45 gca gaa gca gcg ggg ttt tcg ggt tac cct caa acc atc ggc acc ccg 307 Ala u Ala Ala y Phe Ser y Tyr Pro n Thr Ile y Thr Pro 60 gaa ctc cgc gca gcc atc agg ggc gcg ctt gag cgg cgc tac aac atg 355 G u Leu Arg Al a Al a lIe Arg G y Al a Leu G u Arg Arg Tyr Asn IVet 75 80 aca aag ctt gtc gac gcc tcc ctc ctc ccc gtc gtg ggt acc aag gag 403 Thr Lys Leu Val Asp Al a Ser Leu Leu Pro Val Val G y Thr Lys G u 95 100 gca att gcc ctt ctt cca ttc gcg ttg ggt att tcc ggc acc gtt gtc 451 Ala Ile Ala Leu Leu Pro Phe Ala Leu y Ile Ser y Thr Val Val 105 110 115 atc cca gag att gcg tac cca acc tac gaa gtc gct gtc gtg gcc gca 499 Ile Pro G u Ile Ala Tyr Pro Thr Tyr G u Val Ala Val Val Ala Ala 120 125 130 gga tgc acc gtg ttg cgt tct gat tcg ctg ttt aag ctc ggc ccg cag 547 Sy Cys Thr Val Leu Arg Ser Asp Ser Leu Phe Lys Leu y Pro G n 135 140 145 atc ccg tcg atg atg ttt atc aac tca cca tcc aac ccc aca ggc aag 595 Ile Pro Ser IVet IVet Phe Ile Asn Ser Pro Ser Asn Pro Thr G y Lys 150 155 160 165 gtt ctg ggc atc cca cac ttg cgc aag gtt gtg aag tgg gcg cag gaa 643 Val Leu G y lie Pro His Leu Arg Lys Val Val Lys Trp Ala G n G u 170 175 180 aac aac gtg atc ctc gca gct gat gaa tgc tac ttg ggt ctt ggc tgg 691 Asn Asn Val lie Leu Al a Ala Asp G u Cys Tyr Leu y Leu y Trp 185 190 195 gac gat gaa aac cca ccg atc tca att ttg gat cca cgt gtc tgc gat 739 Asp Asp G u Asn Pro Pro lie Ser lie Leu Asp Pro Arg Val Cys Asp 200 205 210 ggc gac cac acc aac ttg atc gcc att cac tcg ctg 775 G y Asp His Thr Asn Leu lie Ala lie His Ser Leu 215 220 225 <210> 128 <211> 225 <212> PRT Page 174 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <400> 128 Met Thr Ser 1 Asp SIle SIle Thr Ar g Val Ser Al a Lys 145 Asn Lys Leu Leu Asn I le Gy Tyr Thr Thr 115 Val Gy Thr Al a Leu 195 Arg Thr Pro Leu 5 Ala Ser Al a Lys Leu Ser Val G y Ala Leu Al a G u Thr Pro G u Leu 70 Asn IVet Thr Lys Lys G u Al a l e 100 Val Val I e Pro Al a Al a G y Cys 135 Pro n lie Pro 150 G y Lys Val Leu 165 n G u Asn Asn 180 G y Trp Asp Asp Val Al a Thr 40 Al a Ar g Leu Al a G u 120 Thr Ser Sy Val G u 200 Ser Lys 25 Pr o Al a Al a Val Leu 105 I le Val Met SIle SIle 185 Asn Val 10 Al a Val Sy Al a Asp 90 Leu Al a Leu Met Pr o 170 Leu Pr o Leu Al a Asp Phe l Ie Al a Pr o Tyr Ar g Phe 155 Hi s Al a Pr o Pr o Ser Pr o Ser Ar g Ser Phe Pr o Ser 140 I le Leu Al a SIle Asp Hi s Val Sy Sy Leu Al a Thr 125 Asp Asn Ar g Asp Ser 205 Phe Pr o Al a Tyr Al a Leu Leu 110 Tyr Ser Ser Lys G u 190 I le Pr o Asp Pr o Pr o Leu Pr o Sy G u Leu Pr o Val 175 Cys Leu Pro Arg Val Cys Asp Gy Asp His Thr Asn Leu lie Ala lie His Ser 210 215 220 Leu 225 <210> 129 <211> 1206 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1183) <223> RXN00508 <400> 129 agccacgtgt ttaaggatag ttgaaagcgt ggggcaatac tggcactaac cccggcacca atcgtatttc tgtccgcggt tggtggcaca atagttcaac atg aac ttg ctg acc 115 IVet Asn Leu Leu Thr 1 acc aaa att gac ctg gat gcc atc gcc cat aac acg agg gtg ctt aaa 163 Thr Lys Ile Asp Leu Asp Ala lie Ala His Asn Thr Arg Val Leu Lys Page 175 seql i st cor r ect ed. t xt 15 caa atg gcg ggt ccg gcg aag ctg atg gcg gtg gtg aag gcg aat gca 211 G n IVet Ala y Pro Ala Lys Leu IVet Ala Val Val Lys Ala Asn Ala 30 tat aac cat ggc gta gag aag gtc gct ccg gtt at t gct gct cat ggt 259 Tyr Asn His G y Val G u Lys Val Ala Pro Val IIe Ala Ala His G y 45 gcg gat gcg ttt ggt gtg gca act ctt gcg gag gct atg cag ttg cgt 307 Ala Asp Ala Phe y Val Ala Thr Leu Ala G u Ala Iet G n Leu Arg 60 gat atc ggc atc agc caa gag gtt ttg tgt tgg att tgg aca ccg gag 355 Asp lie y lie Ser n u Val Leu Cys Trp lie Trp Thr Pro u 75 80 cag gat ttc cgc gcc gcc att gat cgc aat att gat ttg gct gtt att 403 Sn Asp Phe Arg Ala Ala lie Asp Arg Asn Ile Asp Leu Ala Val lie 95 100 tct ccc gcg cat gcc aaa gcc ttg atc gaa act gat gcg gag cat att 451 Ser Pro Ala His Ala Lys Ala Leu lie u Thr Asp Ala u His lie 105 110 115 cgg gtg tcc atc aag att gat tct ggg ttg cat cgt tcg ggt gtg gat 499 Arg Val Ser lie Lys I e Asp Ser G y Leu His Arg Ser G y Val Asp 120 125 130 gag cag gag tgg gag ggc gtg ttc agc gcg ttg gct gct gcc ccg cac 547 G u n G u Trp G u G y Val Phe Ser Ala Leu Ala Ala Ala Pro His 135 140 145 att gag gtc acg ggc atg ttc acg cac ttg gcg tgc gcg gat gag cca 595 lie G u Val Thr G y IVet Phe Thr His Leu Ala Cys Ala Asp G u Pro 150 155 160 165 gag aat ccg gaa act gat cgc caa att att gct ttt cga cgc gcc ctt 643 G u Asn Pro G u Thr Asp Arg n lie lie Ala Phe Arg Arg Ala Leu 170 175 180 gcg ctc gcc cgc aag cac ggg ctt gag tgc ccg gtc aac cac gta tgc 691 Ala Leu Ala Arg Lys His G y Leu G u Cys Pro Val Asn His Val Cys 185 190 195 aac tca cct gca ttc ttg act cga tct gat tta cac atg gag atg gtc 739 Asn Ser Pro Ala Phe Leu Thr Arg Ser Asp Leu His IVet G u Iet Val 200 205 210 cga ccg ggt ttg gcc ttt tat ggg ttg gaa ccc gtg gcg gga ctg gag 787 Arg Pro y Leu Ala Phe Tyr y Leu G u Pro Val Ala y Leu G u 215 220 225 cat ggt ttg aag ccg gcg atg acg tgg gag gcg aag gtg agc gtc gta 835 His G y Leu Lys Pro Al a bet Thr Trp G u Ala Lys Val Ser Val Val 230 235 240 245 aag caa att gaa gct gga caa ggc act tcc tat ggc ctg acc tgg cgc 883 Lys On Ile u Ala Gy Gn Gy Thr Ser Tyr y Leu Thr Trp Arg 250 255 260 gct gag gat cgc ggc ttt gtg gct gtg gtg cct gcg ggc tat gcc gat 931 Al a u Asp Arg y Phe Val Ala Val Val Pro Al a y Tyr Al a Asp 265 270 275 ggc atg ccg cgg cat gcc cag ggg aaa ttc tcc gtc acg att gat ggc 979 G y IVet Pro Arg His Al a G n y Lys Phe Ser Val Thr IIe Asp G y Page 176 ct g Leu tct Ser 310 at a SIle gac Asp cgc Ar g 280 t at Tyr ggc Gy ggt Gy at t SIle t at Tyr 360 seql i st cor r ect ed. t xt 285 290 cag gtt ggg cgc gta tgc atg gat cag G n Val y Arg Val Cys Vet Asp G n 300 305 aat cca cac ggc gtg gaa gct ggg gcg Asn Pro His G y Val G u Ala G y Ala 315 320 aat ggg cat gac gca act gat ttt gcg Asn G y His Asp Al a Thr Asp Phe Al a 330 335 tat gag gta gtg tgc cga cca acc ggc Tyr G u Val Val Cys Arg Pro Thr Gy 350 taagtgaata cgtttaagga gca ttc Phe aag Lys gag G u cga Ar g 355 gtt att Val lie gcc gtg Al a Val 325 cgt tta Arg Leu 340 act gt c Thr Val 1027 1075 1123 1171 1206 <210> 130 <211> 361 <212> PRT <213> Corynebacteri um gl utam cum <400> 130 IVet Asn Leu Leu Thr 1 Thr Val SIle Al a I le Asp Asp Ar g Al a 145 Cys Phe Val Val Al a Al a G n Thr Al a G u 115 Gy Al a Asp Ar g Hi s 195 5 Lys Al a Gy Ar g G u l le SIle Asp Hi s Pr o 165 Leu Cys Thr Lys Sn let Tyr Asn Al a Asp Asp Il e 70 Sn Asp Ser Pro Arg Val G u G n 135 ie G u 150 Su Asn Al a Leu Asn Ser SIle Al a Hi s 40 Al a Sy Phe Al a Ser 120 G u Val Pr o Al a Pr o 200 Asp Leu 10 G y Pro G y Val Phe G y Ile Ser Arg Ala 90 Hi s Al a 105 I e Lys Trp u Thr G y G u Thr 170 Arg Lys 185 Al a Phe Asp Ala lie Ala His Asn Al a G u Val G n Al a Lys SIle Sy Met 155 Asp Hi s Lys Lys Al a G u l Ie Al a Asp Val 140 Phe Ar g Sy Leu Val Thr Val Asp Leu Ser 125 Phe Thr G n Leu Ar g 205 Met Al a Leu Leu Ar g SIle 110 Sy Ser Hi s SIle G u 190 Al a Pr o Al a Cys Asn G u Leu Al a Leu SIe 175 Cys Leu Thr Ser Asp Leu Page 177 Hi s Val 225 Lys Gy Al a Val Asp 305 Gy Phe Thr G u Gy Ser Thr Tyr 275 I le Phe Lys G u Ar g 355 Val G u Val 245 Ar g Asp Gy SIle Val 325 Leu Val Pr o 215 Gy G n G u Met Asp 295 Leu Phe Thr Al a seql i st cor r ect ed. t xt G y Leu Al a Phe Tyr G y 220 Leu Lys Pro Ala IVet Thr 235 I e u Ala y n y 250 Asp Arg y Phe Val Ala 265 Pro Arg His Ala G n G y 280 285 Tyr Pro G n Val y Arg 300 G y Asp Asn Pro His G y 315 G y G u Asn G y Hi s Asp 330 Ile Asn Tyr G u Val Val 345 Tyr Val 360 Leu Tr p Thr Val 270 Lys Val Val Al a Cys 350 G u G u Ser 255 Val Phe Cys G u Thr 335 Ar g <210> 131 <211> 1152 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1129) <223> RXN00636 <400> 131 tcatgggatt cagcaaggcg gccacccgaa ccgtctttgg tcgatctggc ccgttcgaac ataaggaata ttcctactcc cct Pr o at g Met aaa Lys gcc Al a ggc Gy gtt Val gct Al a cac Hi s ggg Gy ggt Gy at t I e gcc Al a at c SIle acc Thr acg Thr cgc Ar g gcc Al a gaa G u gca Al a at c SIle cgc Ar g gag G u gcg Al a at t l Ie at t SIle tta act Leu Thr 15 att gcc I e Al a cag atg G n Met ggc gag G y G u gca t at Al a Tyr 80 Page 178 tggaggagta ggagcgatga atg atg att gat aca IVet IVet Ile Asp Thr 1 gcc aac att tcc agg Ala Asn lie Ser Arg ctg cgt ccg cat gtg Leu Arg Pro His Val cag gtc gac gcc ggt Sn Val Asp Ala y gcg gaa att ttt gcc Ala G u Ile Phe Ala ccg ctg tat cta acc Pro Leu Tyr Leu Thr seql i st cor r ect ed. t xt gat cat gca gtg caa cgc ctg aac gcg atc ccc gga gaa att tcc att 403 Asp His Ala Val G n Arg Leu Asn Ala lie Pro G y G u lie Ser I I e 95 100 ggc gtg gat tcg gta gag atg gca cag gcg acg gcg ggt ttg cgg gaa 451 G y Val Asp Ser Val G u Met Al a n Al a Thr Al a G y Leu Arg G u 105 110 115 gat atc aag gct ctg att gaa gtg gat tcg gga cat cgt aga agt gga 499 Asp IIe Lys Ala Leu lie G u Val Asp Ser G y His Arg Arg Ser G y 120 125 130 gtc acg gcg act gct tca gaa ttg agt cag atc cgc gag gcg ctg ggc 547 Val Thr Ala Thr Ala Ser G u Leu Ser n lie Arg u Ala Leu y 135 140 145 agc agg tat gca gga gtg ttt act ttt cct ggg cat tct tat ggc ccg 595 Ser Arg Tyr Ala G y Val Phe Thr Phe Pro G y His Ser Tyr G y Pro 150 155 160 165 gga aat ggt gag cag gca gca gct gat gag ctt cag gct cta aac aac 643 G y Asn G y u G n Ala Ala Ala Asp G u Leu G n Al a Leu Asn Asn 170 175 180 agc gtc cag cga ctt gct ggc ggc ctg act tct ggc ggt tcc tcg ccg 691 Ser Val G n Arg Leu Al a G y G y Leu Thr Ser G y G y Ser Ser Pro 185 190 195 tct gcg cag ttt aca gac gca atc gat gag atg cga cca ggc gtg tat 739 Ser Ala G n Phe Thr Asp Ala lie Asp G u Iet Arg Pro y Val Tyr 200 205 210 gtg ttt aac gat tcc cag cag atc acc tcg gga gca tgc act gag aag 787 Val Phe Asn Asp Ser G n n Ile Thr Ser y Ala Cys Thr G u Lys 215 220 225 cag gtg gca atg acg gtg ctg tct act gtg gtc agc cga aat gtg tca 835 G n Val Ala Met Thr Val Leu Ser Thr Val Val Ser Arg Asn Val Ser 230 235 240 245 gat cgt cgg atc att ttg gat gcg gga tcc aaa atc ctc agc act gat 883 Asp Arg Arg lie lie Leu Asp Ala y Ser Lys Ile Leu Ser Thr Asp 250 255 260 aaa cca gca tgg att gat ggc aat ggt ttt gtt ctg ggg aat cct gaa 931 Lys Pro Ala Trp lie Asp y Asn y Phe Val Leu y Asn Pro u 265 270 275 gcc cga atc tct gct ttg tcg gag cat cac gca acc att ttc tgg cca 979 Ala Arg lie Ser Ala Leu Ser G u His His Ala Thr Ile Phe Trp Pro 280 285 290 gat aaa gtg cta ctt cca gta atc ggg gag cag ctc aac atc gtg ccc 1027 Asp Lys Val Leu Leu Pro Val IIe G y G u G n Leu Asn IIe Val Pro 295 300 305 aac cat gcc tgc aac gtg att aat ttg gtg gat gag gtc tac gtt cgg 1075 Asn His Ala Cys Asn Val lie Asn Leu Val Asp G u Val Tyr Val Arg 310 315 320 325 gaa gcc gat ggc act ttc cgt acc tgg aag gta gtt gcc cgc ggc aga 1123 G u Ala Asp y Thr Phe Arg Thr Trp Lys Val Val Ala Arg y Arg 330 335 340 aac aat tagggaaacc tcttgacctt cac 1152 Asn Asn Page 179 seql i st cor r ect ed. t xt <210> 132 <211> 343 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 132 lIet lIet Ile Asp Thr Pro Al a Val Leu 1 Al a Leu G n Al a Pr o Gy Al a Hi s Ar g 145 Hi s G n Sy Ar g Al a 225 Ser SIle Leu Thr Leu 305 l Ie Pr o Asp l Ie Tyr SIle Leu 115 Ar g Al a Tyr Leu Ser 195 Sy Thr Asn Ser Asn 275 Phe SIle 5 Ar g Val Sy Al a Thr I e G u Sy Sy Pr o 165 Asn Pr o Tyr Lys Ser 245 Asp G u Pr o Pr o bet Lys Al a Sy 70 Asp Gy Asp Val Ser 150 Sy Ser Ser Val G n 230 Asp Lys Al a Asp Asn 310 Al a Thr Ar g Al a Hi s Val SIle Thr 135 Ar g Asn Val Al a Phe 215 Val Ar g Pr o Ar g Lys 295 Hi s Al a Hi s 40 Sy Sy Al a Asp Lys 120 Al a Tyr Sy G n G n 200 Asn Al a Ar g Al a SIle 280 Val Al a Hi s Lys SIle Phe Val Ser 105 Al a Thr Al a G u Ar g 185 Phe Asp let SIle Tr p 265 Ser Leu Cys I e 10 Al a SIle Thr Thr G n 90 Val Leu Al a Sy G n 170 Leu Thr Ser Thr SIle 250 I le Al a Leu Asn Asp Sy SIle Cys Asp 75 Ar g G u SIle Ser Val 155 Al a Al a Asp G n Val 235 Leu Asp Leu Pr o Val 315 Ar g Al a G u Al a I le Leu Met G u G u 140 Phe Al a Sy Al a G n 220 Leu Asp Sy Ser Val 300 I le G u Hi s SIle Thr Phe Asn Al a Val 125 Leu Thr Al a Gy l e 205 I le Ser Al a Asn G u 285 I le Asn Ar g G u Al a SIle SIle Al a G n 110 Asp Ser Phe Asp Leu 190 Asp Thr Thr Sy Sy 270 Hi s Sy Leu Leu I e G n Sy Al a l Ie Al a Ser G n Pr o G u 175 Thr G u Ser Val Ser 255 Phe Hi s G u Val Thr Al a let G u Tyr Pr o Thr Gy I e Gy 160 Leu Ser let Sy Val 240 Lys Val Al a G n Asp 320 Su Val Tyr Val Arg u Ala Asp Gy Thr Phe Arg Thr Trp Lys Val Page 180 seql i st cor r ect ed. t xt 330 325 Val Ala Arg Gy Arg Asn Asn 340 335 <210> 133 <211> 879 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(856) <223> RXA02536 <400> 133 aagaagtgat cacgcgaacc tgtgtataac ttgcctcaaa gcgtattgcc ttgcttcaga tctcgacgaa ttccgataag gcgcctaggc tgtggattat ct g Leu ttg Leu gat Asp aaa Lys cct Pr o aac Asn at t l Ie ccg Pr o gt g Val gcc Al a 150 ggt Gy cgt Ar g ttt Phe cag G n gcc Al a gac Asp gt g Val aca Thr gat Asp 120 aca Thr aac Asn gga Gy gct Al a gag G u gag G u gag G u gt g Val at t lie gac Asp ctg Leu t ac Ty r gca Al a tta Leu 170 gct Al a gcg Al a gag G u ct g Leu cag G n 75 agt Ser gcg Al a gtt Val gat Asp cag G n 155 gaa G u gaa G u act Thr ct c Leu gac Asp cgc Ar g G y ttc Phe gt a Val at t SIle 140 at a SIle caa G n gct Al a caa G n ggc G y gt c Val gaa G u gga G y t at Tyr 110 gag G u ttc Phe gt g Val gaa G u gcg Al a 15 agc Ser gaa G u at c SIle aaa Lys ttg Leu 95 agg Ar g gt c Val cca Pr o gtt Val gt c Val 175 gaa G u ttt Phe ttc Phe gtt Val acg Thr 80 cat Hi s gaa G u gac Asp gaa G u ccc Pr o 160 ct c Leu at g Met 1 cag G n ggt G y t cc Ser gcg Al a at c SIle cag G n t cc Ser gat Asp cag G n 145 acg Thr cct Pr o gac aac ttc Asp Asn Phe ggg G y acg Thr acc Thr ggc G y tcg Ser gga G y gac Asp at t SIle 130 ttc Phe tcg Ser cgc Ar g gct Al a gga G y gcg Al a at g let cgc Ar g t ac Tyr act Thr 115 aaa Lys aaa Lys tgg Tr p gcg Al a cgg Ar g agg Ar g gt a Val ttc Phe gt c Val aac Asn 100 gt g Val ttt Phe gac Asp caa G n cgt Ar g 180 gcc Al a gtg Val ctt Leu cga Ar g acc Thr aac Asn aaa Lys aaa Lys ggt G y ct c Leu gac Asp 165 gca Al a ctg gat tcc acc tgc tgg atc gta gcg tgt ggg caa gcg cga ctt cca Page 181 Leu gaa G u at g Met cca Pr o 230 egg Ar g Ser tta Leu 200 aca Thr at g Met gca Al a Cys gat Asp cca Pr o at c SIle cct Pr o 250 Tr p gaa G u cac Hi s gcg Al a 235 gtt Val SIle cga Ar g ggt Gy 220 gat Asp ctt Leu seql i st cor r ect ed. t xt Val Ala Cys Gy n Ala Arg Leu Pro 190 195 aaa ggc cct acg ggg att ggt cat tcc Lys y Pro Thr y lie y His Ser 205 210 gaa gta att gct agc gcg ggt tat gag Su Val I e Ala Ser Ala y Tyr u 225 att gat gtc agc ggt ttg gcc aaa att I e Asp Val Ser y Leu Ala Lys Ile 240 245 t aaccact gt ct aaggaat c act <210> 134 <211> 252 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 134 lIet Asp Asn 1 Sn G y Al a G y Thr G y Ser Thr Al a Al a G y lt I e Ser Ar g Sn G y Tyr Ser Asp Thr 115 Asp I e Lys 130 G n Phe Lys 145 Thr Ser Tr p Pro Ar g Al a G n Al a Ar g 195 G y lie y 210 Phe Al a 5 Arg Val Arg Leu Val Arg Phe Thr Val Asn Asn Lys 100 Val Lys Phe G y Asp Leu Sn Asp 165 Arg Ala 180 Leu Pro Hi s Ser Leu Val Thr Leu Al a Thr Hi s Sy Al a 135 Ar g Pr o Asp G u Val 215 Ar g Phe G n 40 Al a Asp Val Thr Asp 120 Thr Asn Sy Ser Leu 200 Thr Asp Pr o 25 Al a Asp Thr Leu Tyr 105 G u Cys Sy Lys Thr 185 Ar g Asn Al a 10 G u G u G u Val SIle 90 Asp Leu Tyr Al a Leu 170 Cys Asp Al a Al a G u Leu G n Ser Al a Val Asp G n 155 G u Tr p G u Lys Ser Asp Val Sy Al a Sy Phe 125 Ar g SIle Tr p Val Lys 205 Al a G n Sy Val G u Sy Tyr 110 G u Phe Val G u Al a 190 Sy Al a Ser G u SIe Lys Leu Ar g Val Pr o Val Val 175 Cys Pr o Pro His G 2 Page 182 Su Val I e Ala seqI i st car r ect ed. t xt Ser Ala G1y Tyr Ou Pro Ou I'bt Leu IlieAla Asp lieAsp Val Ser 225 230 235 240 G31y Leu Ala Lys Ilie Ar g Ou Ala Leu Pro Val Leu 245 250 <210O> 135 <21 1> 1635 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1612) <223> RXS00870 <400> 135 caagacggcg atgtcgccgc cgctgttgat accgcagcgc caacaat tca ct tcgcagag cat t taagga at t tacacac gact tgt tca cacagat at t atg tct gaa cca caa 11! I'bt Ser O31u Pro O31n ac c Thr aag Ly s gt t ValI ac c Thr gct Al a ct g Le u at g vb t tt c Ph e at t I Ie ag c Ser 1 50 gca Al a t cg Ser gct Al a ct g Le u gct Al a ct t Le u gag O31u g aa O31u c ac Hi s 120 gt g ValI tt c Ph e gct Al a t gg Tr p gt c ValI ag c Ser aag Ly s aac As n at c I Ie ct g Le u ct t Le u t cc Ser tt c Ph e ggc G31y 1 70 at t I Ie t ac Tyr cag O31n ac g Thr t t c Ph e 75 act Thr cgc Ar g aaa Ly s t t g Le u cct Pr o 155 aac As n g ac As p aat As n g aa O31u t gg Tr p cgt Ar g gca Al a ggc G31y ggt G31y aag Ly s 140 gcg Al a gca Al a gcg Al a gca Al a at c I Ie aac As n ct g Le u c ac Hi s g aa O31u 110 tt c Ph e cca Pr o gt g ValI at t I Ie t cc Ser ggc G31y gcc Al a t ct Ser aat As n 80 aag Ly s gt g ValI gag O31u ggt G31y at g vb t 1 60 aag Ly s 1 cca Pr o c ag O31n ac c Thr at c I Ie 65 gct Al a gt c ValI gag (31u aac As n gt t ValI 145 t gg Tr p cct Pr o t cc Ser gt c ValI at c I Ie 50 gct Al a cgc Ar g tt g Le u ct t Le u gt c ValI 130 gt c ValI tt t Ph e t ca Ser t cc Ser 20 gcc Al a t ct Ser cgc Ar g ggt G31y g at As p 1 00 ac c Thr ac c Thr at c I Ie cca Pr a aag Ly s 1 ggc G y aat As n gcc Al a c aa 0On gag 0Ou gcc Al a ggt 0Oy ggc 0Oy at c I Ie at c I Ie 1 g at As p Page 183 seql i st cor r ect ed. t xt cct tcg gca gcg ctg tgg atg gct Pro Ser Ala Ala Leu Trp IVet Ala 185 cag atc tgg aag On lie Trp Lys gaa gct ggt ctt G u Al a G y Leu cca Pr o ggt Sy acc Thr 230 cgc Ar g gat Asp ggc Gy gaa G u acc Thr 310 ct g Leu gt c Val aac Asn ctt Leu ttc Phe 390 at t SIle acc Thr ggc Gy ggc Gy 200 ct g Leu at c SIle cag G n gat Asp gcc Al a 280 gtt Val cgc Ar g cca Pr o at c SIle gcc Al a 360 gac Asp ccg Pr o ct g Leu gat Asp at c SIle 440 ttc Phe agc Ser aag Lys ttg Leu 250 gat Asp gag Gu gac Asp ggc Gy at c SIle 330 gag G u gac Asp at c SIle ct c Leu aac Asn 410 gga Gy at c SIle aac Asn cct Pr o tac Tyr 235 ggc Gy ct g Leu cgt Ar g gag G u aac Asn 315 acc Thr gcc Al a ggg Gy cca Pr o tct Ser 395 t cc Ser gcg Al a aac Asn gt g Val gat Asp 220 at c SIle ggc Gy gtt Val tgc Cys ct c Leu 300 ggt Gy gac Asp gac Asp cac Hi s ct c Leu 380 gt c Val ggt Gy gca Al a gt a Val ct c Leu 205 gt c Val t ac Tyr gcg Al a gcc Al a at g Met 285 at t SIle gcc Al a gt c Val ggc Gy gag G u 365 acg Thr gtt Val gaa G u cgc Ar g cca Pr o 445 190 cag G n tct Ser gag G u aag Lys gat Asp 270 gct Al a gag G u ggc Gy cac Hi s gcc Al a 350 gag G u ttc Phe cgt Ar g ttc Phe cgc Ar g 430 at c SIle ggc Gy gcg Al a act Thr aac Asn 255 cag G n gtt Val aag Lys gac Asp cgc Ar g 335 aag Lys ggc Gy cgc Ar g gt c Val ggc Gy 415 ttc Phe cca Pr o gac Asp at t l Ie t cc Ser 240 cac Hi s gca Al a tct Ser at c SIle gag G u 320 gac Asp at c SIle ttc Phe gcc Al a gca Al a 400 aac Asn cag G n gt g Val aag Lys t cc Ser 225 gcg Al a at g Met at c l Ie gt g Val aag Lys 305 cag G n aag Lys at c SIle ttc Phe t ac Tyr 385 t cc Ser gga G y cat Hi s ct g Leu 210 ttc Phe aag Lys ct g Leu aac Asn gt c Val 290 gag G u ggc Sy gt c Val gt g Val ttc Phe 370 acc Thr ttc Phe acc Thr gag G u 195 gct Al a gt g Val aac Asn gt g Val gca Al a 275 ttg Leu cgc Ar g gag G u gct Al a gac Asp 355 ggc Sy gaa G u gac Asp gca Al a at c SIle 435 gtt Val ggt G y ggc G y ct g Leu 260 ggt G y gct Al a at c SIle ccg Pr o tct Ser 340 ggg Sy cct Pr o gaa G u gag G u at c l Ie 420 gaa G u gat Asp t cc Ser aag Lys 245 cca Pr o t ac Tyr at t SIe gac Asp cac Hi s 325 t at Tyr cgt Ar g acg Thr at c SIe gca Al a 405 ttc Phe gt g Val 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 cct gtt gcg tac cac Pro Val Ala Tyr His 450 Page 184 seqI i st car r ect ed. t xt t cct tc ggt ggt t gg aag aac tccct ct tc ggt gac gcc aag gca tat Ser Phe G31y G31y Tr p Lys Asn Ser Leu Phe G31y Asp Ala Lys Ala Tyr 455 460 465 ggc act caa ggt t tt gat t tc t tc acc agg gaa aag gcg at c acc agc G31y Thr (31n G31y Phe Asp Phe Phe Thr Ar g G31u Lys Ala Ilie Thr Ser 470 475 480 485 cgt t gg ct c gac cca gca acc cac ggt ggc at t aac ct c ggt t tc cca Ar g Tr p Leu Asp Pr o Ala Thr His G31y 31y Ilie Asn Leu G31y Phe Pr o 490 495 500 cag aac gat t aat tgaagg agagcacagg act O31n Asn Asp <210> 136 <211> 504 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1 507 1 555 1 603 1 635 <400> 136 I'b t Se r O u Pr o O n Th r I Ie Se r Hi s Pr o O31n Thr I Ie A a ValI O31u As n ValI 145 Tr p Pr o Ser 225 Thr Thr A a Lys5 Lys5 Ser A a 115 Ser O31y Ph e O31u A a 1 95 A a ValI G31y As n A a O31n (31u A a G31y G31y I Ie I Ie 1 65 As p Le u As p Ser Lys5 ValI Thr A a 70 Le u vb t Ph e I Ie Ser 150 A a Pr o Pr o G31y Thr 230 Thr A a Ly s ValI A a G31y Pr a As p 135 Pr a I Ie Ser As p Le u 215 Pr a A a Le u 40 A a Le u (31u (31u Hi s 120 ValI Ph e A a A a G31y 200 Le u I Ie Pr a A a A a Ph e I Ie I Ie 1 05 Le u Tyr As n A a A a 1 85 ValI As n A a Tr p I Ie 10 Val Ty r Ser 03 n Lys Thr As n Phe 1 1 e Thr 90 Leu Arg Leu Lys Ser Leu Phe Pr o 1 31y As n 1 70 Leu Trp Phe As n Ser Pr o Lys Tyr 235 As p As n (31u Tr p Ar g A a G31y G31y Ly s 140 A a A a vb t ValI As p 220 G31y Pr a (31u G31y (31u (31u O31n A a 125 O31n vb t ValI A a Le u 205 ValI A a A a li1e As n Le u Hi s O31u 110 Ph e Pr a ValI I Ie O31n 1 O31n Ser lIe Thr As p Le u Le u O31y ValI As n Le u Pr a Le u 1 li1e O31y A a I I e Tyr 03 u Thr Ser Page 185 seql i st cor r ect ed. t xt Ala Lys Asn Gy Lys Arg Val n Ala Leu Gy Gy Al 245 250 a Lys Asn His 255 lbet SIle Val Lys 305 G n Lys SIle Phe Tyr 385 Ser Sy Hi s Pr o Asp 465 Lys Asn Val Al a 275 Leu Ar g G u Al a Asp 355 Gy G u Asp Al a SIle 435 Al a Lys SIle Gy Pr o Tyr SIle Asp Hi s 325 Tyr Ar g Thr SIle Al a 405 Phe Val Hi s Tyr Ser 485 Pr o Al a Al a Ser 295 Leu Gy Asp Cys SIle 375 Gy G u Asn let Phe 455 Thr Tr p Asn Asp Al a 280 Val Ar g Pr o SIle Al a 360 Asp Pr o Leu Asp SI e 440 Gy G n Leu Asp Leu 265 Gy Al a SIle Leu Al a 345 Val Asp Val SIle Gy 425 Gy Gy Gy Asp Leu Ar g G u Asn 315 Thr Al a Gy Pr o Ser 395 Ser Al a Asn Lys Asp 475 Al a Al a let 285 I le Al a Val Gy G u 365 Thr Val G u Ar g Pr o 445 Ser Phe Hi s Asp 270 Al a G u Gy Hi s Al a 350 G u Phe Ar g Phe Ar g 430 I le Leu Thr Sy G n Val Lys Asp Ar g 335 Lys Gy Ar g Val Gy 415 Phe Pr o Phe Ar g Sy 495 Al a Ser SIe G u 320 Asp SIe Phe Al a Al a 400 Asn G n Val Gy G u 480 I e <210> 137 <211> 531 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(508) <223> RXS02299 <400> 137 acgcgggggt tgt t gccgga tcgaaatatt cctttcct t g tcatctcacg ctatgatttc taaaacttgc aggacaaccc ccataaggac accacaggac atg ctg cgc acc atc 115 Iet Leu Arg Thr Ile 1 Page 186 seql i st cor r ect ed. t xt ctc gga agt aag att cac cga gcc act g Leu G y Ser Lys lie His Arg Ala Thr V tat gtt ggc tct gta acc atc gac gcc g Tyr Val Gy Ser Val Thr Ile Asp Ala A 30 ttg atc gaa ggc gaa aaa gtt gcc atc g Leu ie G u y G u Lys Val Ala Ile V 45 cgt ctg gaa act tat gtc att gtg ggc g Arg Leu G u Thr Tyr Val Ile Val Gy A 60 tgc atc aat ggt gcc gct gca cac ctt a Cys lie Asn G y Ala Ala Ala His Leu I 75 atc atc atg agc tac ctt cag gca act g Ile Ile IVet Ser Tyr Leu n Ala Thr A gag cca aag att gtg cac gtg gac gcc g Su Pro Lys Ile Val His Val Asp Ala A 105 110 ggc aac gat ctt gcg gaa gca cta cct g Sy Asn Asp Leu Ala G u Ala Leu Pro C 120 125 aga agc att tagcgtttta gctcgccaat att Arg Ser Ile 135 <210> 138 <211> 136 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 138 NIet Leu Arg Thr Ile Leu Gy Ser Lys I 1 5 G n Ala Asp Leu Asp Tyr Val y Ser V 25 Val His Ala Ala G y Leu I I e u G y C 40 Ile Thr Asn Gy Ala Arg Leu u Thr T 55 G y Thr G y Asn I e Cys I e Asn G y A 70 Pro y Asp Leu Val Ile Ile IVet Ser T Su Ala Lys Ala Tyr u Pro Lys Ile V 100 105 Arg Ile Val Ala Leu G y Asn Asp Leu A 115 120 F tc 'al 15 ac sp ta 'al ac sp tt le at sp 95 ac sp ga Sy act caa gct gat Thr G n Ala Asp ctg gtt cac gcc Leu Val His Ala gac atc acc aac Asp Ile Thr Asn gcc gga acg ggc Al a G y Thr G y aat cct ggc gat Asn Pro G y Asp 80 gcg gaa gcc aag Al a G u Al a Lys aac cgc atc gtt Asn Arg lie Val 115 tcc ggg ctt ttg Ser G y Leu Leu 130 ct a gat Leu Asp gcc gga Al a y ggc gct G y Al a aat att Asn I e ctt gtg Leu Val gcg tat Al a Tyr 100 gcg ctc Al a Leu acg tcg Thr Ser le His Ar g 10 'al Thr Il e Su Lys Val yr Val I e l a Al a Al a yr Leu G n 90 'al Hi s Val I a G u Al a 'age 187 Al a Asp Al a Val Hi s Al a Asp Leu 125 Thr Al a l le Gy Leu Thr Al a 110 Pr o Val Asp Val Asp SIe Asp Asp Gy seal i st cor r ect ed. t xt Sy Leu Leu Thr Ser Arg Ser Ile 130 135 <210> 139 <211> 1053 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1030) <223> RXA01561 <400> 139 gtgcccagaa attctgcttg cactcaccca agccgtttag taataatgtt cattttcatc gagttctaga aaacacaggc caaat t gaac ctcacgttca gat Asp cca Pr a gca Al a aac Asn ggc G y gcc Al a gcc Al a caa G n ttt Phe gac Asp 150 ctt Leu t at Tyr at c SIle ttc Phe ttc Phe cag G n gt c Val gca Al a caa G n at c SIle 120 tcg Ser gca Al a gac Asp gga G y gcc Al a gca Al a caa G n gca Al a gca Al a tta Leu aaa Lys tct Ser act Thr gga G y gac Asp 170 gca Al a caa G n gac Asp aag Lys gct Al a t ca Ser 75 agc Ser gtt Val gaa G u ggt G y gca Al a 155 acc Thr gcc Al a caa G n ccc Pr a tgc Cys tcg Ser ggc G y gtt Val gat Asp t at Tyr gct Al a 140 ggc G y at c SIle gt c Val cga Ar g at c SIle ggc G y 45 gaa G u gga G y ccc Pr a cgc Ar g gcg Al a 125 ct g Leu gt c Val gt g Val gt a Val acc Thr gac Asp gt g Val aac Asn aac Asn gcc Al a ct c Leu 110 gaa G u ttt Phe at t SIle gtt Val gca Al a 190 gcc Al a 15 ggc G y ttc Phe gga G y gca Al a acg Thr 95 aag Lys gca Al a tgc Cys ggg G y gct Al a 175 gcc Al a cct Pr a aca Thr aaa Lys ct a Leu gga G y 80 gt a Val caa G n ttt Phe cac Hi s ct a Leu 160 gt c Val cac Hi s at g let 1 cat Hi s caa G n acg Thr ct c Leu ct c Leu ttg Leu t ac Tyr gag G u gcc Al a 145 gaa G u ggt G y gac Asp gtt Val at c SIle cgt Ar g gac Asp gca Al a gt g Val ggt G y gca Al a 130 t ac Tyr at t SIle ggc G y at c SIle ctc acc ctc aac Leu Thr Leu Asn cga Ar g tgg Tr p gga G y cca Pr a aat Asn ccc Pr a gca Al a 115 gct Al a gac Asp gt c Val ggt G y aaa Lys 195 cga Ar g at c SIle gca Al a acg Thr gct Al a gaa G u 100 acc Thr caa G n cag G n gaa G u gga G y 180 gt g Val acg Thr aaa Lys ttc Phe gtt Val ttt Phe act Thr gtg Val acc Thr ccc Pr o gat Asp 165 ct c Leu gtg Val Page 188 seql i st cor r ect ed. t xt gcc gtt gaa ccc tcc aaa att cca acc ctg cac aac tca Ala Val G u Pro Ser Lys lie Pro Thr Leu His Asn Ser 200 205 210 ggc caa cca gtc gat gtg aac gtt tct ggt atc gcg gca G y Gn Pro Val Asp Val Asn Val Ser G y le Ala Ala 215 220 225 ggg gct cgc caa att gga cga gaa gcc ttt gac atc gca G y Ala Arg Gn lie G y Arg G u Ala Phe Asp lie Al a 230 235 240 ccc cca ata ggc gtc cta gtg gac gat gaa gca atc at c Pro Pro lie y Val Leu Val Asp Asp u Ala lie lie 250 255 cgc cac ctc tgg gac aac tac cgc atc cct gcc gag cat Arg His Leu Trp Asp Asn Tyr Arg lie Pro Ala u Hi s 265 270 gca gca ctc gcc tct ctt acc agt gga gca tac aaa cct Ala Ala Leu Ala Ser Leu Thr Ser y Ala Tyr Lys Pro 280 285 290 gaa aaa gtg gca gtc att gtg tgc gga gcg aac act gac G u Lys Val Ala Val I e Val Cys y Ala Asn Thr Asp 295 300 305 ctg tgatgtgatt tcaaacgatc aca Leu 310 <210> 140 <211> 310 <212> PRT <213> Cor ynebact eri um gl ut arri cum ct c Leu gat Asp act Thr gca Al a ggc G y 275 gca Al a ct c Leu at t SIle t ct Ser gcc Al a gct Al a 260 gct Al a gca Al a aca Thr gcc Al a ttg Leu cat Hi s 245 cga Ar g gcc Al a gat Asp aca Thr 739 787 835 883 931 979 1027 1053 <400> 140 IVet Leu Thr Leu Asn Asp Val lie Thr Ala G n G n 1 Hi s G n Thr Leu Leu Leu Tyr a u Al a Ar g Tr p Sy Pr o Asn Pr o Al a 115 Al a Asp 5 Thr Lys Phe Val Phe Thr Val Thr Pr o Pr o Al a Asn Sy 70 Al a Al a G n Phe Asp Leu G u Ar g I le Al a Pr o G n G u 135 I le G u Leu Leu Al a Ser Val 105 a y G u Al a 10 Al a Asp Pro G n Lys Cys Al a Al a Ser Al a Ser G y Leu Ser Val 90 Lys Val Asp Ser G u Tyr Thr G y Al a 140 G y Al a G y Page 189 Ar g SIle Sy G u Sy Pr o Ar g Al a 125 Leu Val Al a Sy Phe Sy Al a Thr Lys Al a Cys Sy seql i st cor r ec 145 150 155 G u Ile Val G u Asp Leu Pro Asp Val Asp Thr 165 170 G y G y G y G y Leu Tyr Al a G y I e Al a Al a 180 185 Asp Ile Lys Val Val Al a Val G u Pro Ser Lys 195 200 Asn Ser Leu lie Ala G y G n Pro Val Asp Val 210 215 Ala Ala Asp Ser Leu Gy Ala Arg Gn lle Gy 225 230 235 lie Ala Thr Ala His Pro Pro Ile G y Val Leu 245 250 lie lie Ala Ala Arg Arg His Leu Trp Asp Asn 260 265 G u His G y Ala Ala Al a Ala Leu Ala Ser Leu 275 280 Lys Pro Ala Ala Asp G u Lys Val Ala Val lie 290 295 Thr Asp Leu Thr Thr Leu 305 310 <210> 141 <211> 1470 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1447) <223> RXA01850 <400> 141 ttcgtgcaac ttcagactct tacggaggcg atggaccaaa tcaccttgta caccaccaga gaaaaggccc accctcagcc ted. txt Ile Val Val Val Ile Pro 205 Asn Val 220 Arg G u Val Asp Tyr Arg Thr Ser 285 Val Cys 300 Val Al a 190 Thr Ser Al a Asp SIle 270 Gy Gy Al a 175 Al a Leu Gy Phe G u 255 Pr o Al a Al a gtt Val ggc Gy cat Hi s cac Hi s acg Thr ct a Leu at g Met gat Asp act Thr gtt Val agc Ser gcc Al a acg Thr cgg Ar g at t SIle at c SIle gcc Al a tt g Leu gcg Al a gat Asp ggt Gy ct c Leu cac Hi s gt a Val gct Al a at c SIle acg Thr gga Gy 45 tta Leu gca Al a gga Gy t at Tyr t cc Ser ct g Leu ccc Pr o cca t ca Pro Ser 15 at c t ct Ile Ser ctt gcc Leu Al a ggt ctg G y Leu t ca ccc Ser Pro Page 19C aacaactaca atcaagcaga atg gct atc agt gtt bet Ala I e Ser Val 1 tcc tca cat acc gtc Ser Ser His Thr Val gaa ttt ccc agc tcg G u Phe Pro Ser Ser gcc acc ggt aaa ggc Al a Thr G y Lys G y gtg gga tgg gaa cca Val Gy Trp G u Pro ggc gcg ccg att cct Gy Ala Pro lle Pro seql i st cor r ect ed. t xt gcg Al a acg Thr aaa Lys at g Met t ca Ser 150 t cc Ser aat Asn gat Asp acg Thr cag G n 230 gat Asp gaa G u gct Al a aca Thr gcg Al a 310 gt g Val ggt Gy gat Asp t ca Ser 120 ttg Leu at t SIle tta Leu cgc Ar g gt g Val 200 ggg Gy cac Hi s gat Asp aac Asn ggg Gy 280 ttt Phe ggc Gy tgt Cys gt g Val cat Hi s aca Thr gat Asp cca Pr o gca Al a 170 at c SIle cag G n tta Leu ct g Leu gt g Val 250 gct Al a at t SIle gcg Al a at c SIle ggt Sy 330 ggg G y ctt Leu act Thr cgg Ar g 140 gca Al a ggt Sy ggg G y at g Met ggt G y 220 agc Ser tgg Tr p ggt Gy gcg Al a cag G n 300 aag Lys gtt Val cca Pr o cca Pr o t at Tyr 125 aag Lys gag G u cgc Ar g gat Asp cag G n 205 ggg G y aac Asn gt g Val cgt Ar g gt g Val 285 gcg Al a gaa G u ggt Sy aag Lys gaa G u 110 ttg Leu ct g Leu gt g Val gat Asp ctt Leu 190 gag G u ttg Leu ggg G y aat Asn gt g Val 270 at g Met cgg Ar g aat Asn t ca Ser gga G y 95 cac Hi s tcg Ser gac Asp cct Pr o ttt Phe 175 ggc G y tgc Cys aat Asn gat Asp ct g Leu 255 gtt Val cac Hi s acg Thr gcc Al a gcg Al a 335 acg Thr ccc Pr o gt g Val gat Asp tgt Cys 160 gcg Al a aca Thr gt g Val gt g Val acg Thr 240 t ac Tyr act Thr t at Tyr ttt Phe tcg Ser 320 t cc Ser gt g Val aat Asn ggt Sy at c SIle 145 cct Pr o gag G u gt g Val gca Al a caa G n 225 tgt Cys gcc Al a gct Al a gcg Al a ttg Leu 305 at c SIle gcg Al a tcg Ser gcc Al a ggt Sy 130 gga Sy ttt Phe gt c Val gat Asp caa G n 210 cgt Ar g gag G u ttg Leu ccg Pr o cgg Ar g 290 t at Tyr tct Ser at g let t at Tyr gtt Val 115 ggg Sy t ca Ser cag G n at g Met gcc Al a 195 ggc G y cgg Ar g ct g Leu gcg Al a act Thr 275 gat Asp acc Thr ggc Sy gcg Al a t cc Ser 100 acc Thr ttc Phe ggt Sy aag Lys aag Lys 180 cat Hi s at c SIle gcg Al a ggt G y gt g Val 260 aat Asn ttt Phe gcg Al a gcg Al a gct Al a 340 ct g Leu ttt Phe at t SIe gt g Val agt Ser 165 gat Asp ttg Leu gca Al a ccg Pr o gct Al a 245 aat Asn ggt Sy ttg Leu ggt Sy gag G u 325 gcc Al a 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 ggg ttg tgt gca gtc G y Leu Cys Al a Val tta ggt ggt tct ccg caa cag gtg gaa aac gcc Leu G y y Ser Pro n G n Val u Asn Ala Page 191 345 gcg gag at t gcg t tg gag cac A a G3 u I I e A a Leu G3 u Hi s 360 ggc ggg t ta gt g cag at t ccg G31y G31y Leu Val O31n I Ie Pr o 375 380 at g aag t cc at c aat gcg gca l'b t Lys Ser I Ie As n Ala Ala 390 395 aat cgc gt g agt t tg gat gat As n Ar g Val Ser Leu As p As p 410 cgg gac at g ct g acc aaa t at Ar g As p l'b t Leu Thr Lys Ty r 425 acc acc t tg ggc t tc ccg gt g Thr Thr Leu G31y Phe Pr o Val 440 gct t taacac ggc <210O> 142 <21 1> 449 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ar seqi i st car r ect ed. t xt 350 355 aat t tg gga t tg acg t gc gat ccg As n Leu G31y Leu Thr Cys As p Pr o 365 370 t gt at t gaa cgc aac gct at t gct Cys I Ie 03 u Ar g As n A a I Ie A a 385 agg ct t gcc cgg at t ggt gat ggc Ar g Leu Ala Ar g IIe G31y Asp G31y 400 gt g gt g gt c acg at g gct gcc acc Val Val Val Thr l'b t A a A a Thr 415 420 aag gaa acg t cc ct t ggt ggt tt g Lys G31u Thr Ser Leu G31y G31y Leu 430 435 t cg at g acg gag t gt t agcggt acg Ser l'b t Thr 03 u Cys 445 gt g ValI gcc A a aac As n 405 ggC G31y gca A a Ser Ser A a Le u Pr o Thr Pr o ValI As p Cys 1 1219 1267 1315 1363 1411 1457 1470 <400> 142 vb t Ser G31u A a ValI G31y ValI As n G31y I Ie 145 Pr o A a I I e Se r Ser Hi s Th r 2 C Phe Pr o Ser Thr 03 y LyE G31y Tr p (31L Ala Pr o I E Ser Ty r Ser 1 OC A a Val Thr 115 O3 y 03 y PhE 130 O3 y Ser 031 Phe 03 n LvE Val Val Val G31y Ser Hi s O3 y Hi s Pr o Thr 70 Pr o Ala Leu Thr Phe Lys I e l'b t Val Ser 150 Ser Ser 1 65 As p Pr a ValI CYS 55 li1e Ly s Ph e G31y Thr 135 Thr O31n Ph e Ar g li1e As p Pr a Ser Pr a 1 Thr G31u Hi s Le u Ser 10 A a Thr Ar g I Ie ValI 90 Hi s Thr As p Pr a I Ie A a Le u A a As p 75 As n Pr a Ar g Ph e G31u 1 55 G31y Le u Hi s ValI A a G31y Le u Thr Ar g 140 A a I Ie Thr G31y Le u A a Pr a Pr a Tyr 125 Ly s G31u Pr a lie Le u G31y Ser G31y Hi s Ser As p Pr a Ala Tyr Gy Arg Asp 1 70 Phe A a 1 Page 192 (31u Val I'bt Lys Asp 1 80 seqI i st car r ect ed. t xt Asn Ou Arg Leu Ilie His Gy Asp Leu 03 y Thr 1 ValI A a O31n 225 cys A a A a A a Le u 305 li1e A a O31n Thr As n 385 li1e vb t Le u Cys A a 1 95 G31y Ar g Le u A a Thr 275 As p Thr G31y A a O31u 355 As p I Ie As p A a G31y 435 Le u A a Pr o A a 245 As n G31y Le u G31y O31u 325 A a A a ValI A a As n 405 G31y A a As p Thr O31n 230 As p (31u A a Thr A a 310 ValI G31y A a G31y vb t 390 As n Ar g Thr Ar g Pr o 215 ValI Le u (31u A a G31y 295 ValI G31y Le u (31u G31y 375 Lys5 Ar g As p Thr 1 85 Tr p I Ie A a A a A a 265 li1e Gy le O31n A a 345 A a ValI I Ie Ser Le u 425 (31y O31n Le u Le u ValI 250 A a I Ie A a I Ie O31y 330 ValI Le u O31n As n Le u 410 Thr Ph e I Ie Pr o I Ie 235 O31u O31y Pr o O31u I Ie 315 O31u Le u Ou le A a 395 As p Lys5 Pr o O31n 205 O31y As n ValI Ar g ValI 285 A a O31u O31y O31y As n 365 cys Ar g ValI Lys5 Ser 445 cys As n As p Le u 255 ValI Hi s Thr A a A a 335 Pr a O31y O31u A a ValI 415 Thr Thr <210O> 143 <21 1> 1425 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1402) <223> RXA00580 <400> 143 t t t t at at at gggt at cggc ggt ct at gct t gt gggcgt a cct gt cccgc gagt gaggt c Page 193 seql i st cor r ect ed. t xt ttacgcgcgg gattcgtctt gtgaaaggtt agctgacctg caa G n gt g Val gag G u cag G n cgt Ar g cgt Ar g cct Pr o gag G u ttg Leu gtt Val 150 gtt Val tgg Tr p gct Al a ggt Gy gtt Val 230 at c l Ie gac Asp gct Al a at c I e tct Ser t ac Tyr cgt Ar g tct Ser ggc Gy 120 cac Hi s t ac Tyr gag G u gca Al a gaa G u 200 gtt Val tct Ser ct g Leu gt c Val at c l Ie tct Ser ctt Leu ggt Gy aag Lys gca Al a aag Lys at g Met gtt Val gct Al a 170 cct Pr o ggc Gy gct Al a act Thr aag Lys 250 cgt Ar g gct Al a gag G u acc Thr tgc Cys 75 gct Al a cag G n at c SIle aag Lys gat Asp 155 ct g Leu cgc Ar g gcg Al a ggt Gy gt c Val 235 cag G n t ac Tyr ggg Gy aac Asn aat Asn gaa G u ct c Leu gct Al a at g Met ttg Leu 140 cct Pr o aag Lys cac Hi s aag Lys ttg Leu 220 cac Hi s gag G u cag G n gaa G u ttc Phe 45 aag Lys caa G n ttc Phe aat Asn ggt Sy 125 aac Asn gag G u gag G u ctt Leu ct g Leu 205 cac Hi s aag Lys t ac Tyr cca Pr o ctt Leu gtt Val t at Tyr gtt Val ggt Gy gct Al a 110 ct g Leu ttc Phe acc Thr cag G n gat Asp 190 tgg Tr p cca Pr o act Thr gcg Al a ct g Leu 15 gcc Al a ccc Pr o gcc Al a gac Asp gca Al a 95 gct Al a tct Ser t cc Ser at g Met cca Pr o 175 ttc Phe gt c Val agc Ser ttg Leu aag Lys 255 aac Asn cgt Ar g cgt Ar g gag G u at c lile 80 gag Gu gt g Val ttg Leu gga Sy cgt Ar g 160 aag Lys gag G u gat Asp cca Pr o ggt G y 240 aag Lys at g let 1 gag G u caa G n tct Ser ggt G y at t SIle ttc Phe ct g Leu gct Al a aag Lys 145 gtt Val gt a Val gct Al a at g lbt gtt Val 225 gga G y ct g Leu ctt Leu cgc Ar g gtt Val t ac Tyr gag G u gcc Al a at g Met cat Hi s 130 ct g Leu gat Asp at t SIle ttc Phe gct Al a 210 cct Pr o cct Pr o aac Asn cct Pr o aca Thr cag G n ggc G y ctt Leu gtt Val 100 ttg Leu ggt Sy gag G u gat Asp gct Al a 180 tct Ser ttc Phe tct Ser t cc Ser t cc Ser 260 gag G u tta Leu gcg Al a cgc Ar g gca Al a cag G n gct Al a cac Hi s gtt Val cag G n 165 ggc Sy att SIe gct Al a gat Asp ggc G y 245 gta Val acc gat gcc cac Thr Asp Ala His Page 194 seql i st cor r ect ed. t xt ttc cca ggt cag cag ggt ggt cct Phe Pro G y Gn Gn Gy Gy Pro 265 ttg atg Leu IVet cac gca gtt gct gcg aag His Ala Val Ala Ala Lys gct Al a gct Al a tct Ser 310 gt g Val cag G n gcg Al a at t SIle act Thr 390 gca Al a t ac Tyr tct Ser 280 acg Thr gcg Al a ttg Leu gaa G u cct Pr o 360 act Thr gtt Val at t l Ie ct g Leu aag Lys gag G u gcc Al a ttg Leu 330 ct g Leu gat Asp gcg Al a gac Asp tct Ser 410 at t SIle ggt Gy gct Al a 315 gct Al a ct g Leu cct Pr o ct g Leu at c SIle 395 ct g Leu gct Al a gct Al a 300 ggc Gy gat Asp cac Hi s cgt Ar g gct Al a 380 at t SIle cgt Ar g ggc Gy 285 cgc Ar g gt g Val ct g Leu gag G u cca Pr o 365 acc Thr ggt Gy ggc Gy 270 act Thr at t SIle gat Asp cgt Ar g gtt Val 350 cca Pr o cgt Ar g act Thr cgt Ar g gac Asp 430 gag G u ctt Leu gt c Val aac Asn 335 ggt Gy at g Met ggt Gy gct Al a gt a Val 415 cag G n gct Al a ttg Leu 320 t cc Ser at c SIle gtt Val ttc Phe ttg Leu 400 gca Al a cgt Ar g 290 cgt Ar g ggt Gy at g Met gt g Val t ct Ser 370 at t SIle aat Asn ctt Leu 275 gac Asp ct g Leu ggc Gy gat Asp aac Asn 355 ggt Gy cct Pr o ggt Gy gct Al a cgt Ar g act Thr act Thr ggc Gy 340 cgt Ar g ct g Leu gca Al a aag Lys gca Al a 420 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1412 1425 gag ggc ttg gaa G u G y Leu G u tgg acc atc gt c Trp Thr Ile Val taagt t t t t c tttgagtttt cat <210> 144 <211> 434 <212> PRT <213> Corynebacter i um gl ut am cum <400> 144 lIet Thr Asp Ala His G n Ala Asp Asp 1 5 G u Leu Asp Pro G u Val Ala Ala Ala 25 Sn Arg Asp Thr Leu u IVet lie Ala 40 Ser Val Leu G n Ala G n y Ser Val 55 Sy Tyr Pro y Arg Arg Tyr Tyr y 70 Val Arg Tyr G n Pro Leu Asn 10 lie Ala Oy u Leu Ala Arg Ser G u Asn Phe Val Pro Arg Leu Thr Asn Lys Tyr Ala G u Sy Cys u n Val Asp Ile 75 Page 195 seqI i st car r ect ed. t xt Ilie Ou Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe 90 (3 y Al a G3 Ph e Le u Al a Ly s 145 ValI ValI Al a vb t ValI 225 O31y Le u Al a Ph e O31u 305 Thr O31n Thr Thr As p 385 Al a Ly s I Ie As n Thr 115 O31y Tyr vb t I Ie O31n 1 95 Hi s Tyr Ar g Ser Al a 275 As p Le u O31y As p As n 355 O31y Pr o O31y Al a O31n Al a Hi s ValI O31n 1 65 Oy le Al a As p O31y 245 ValI Ly s O31n Al a As p 325 O31n As n Ar g Ph e Ser 405 As p Pr o O31u Le u ValI 150 ValI Tr p Al a O31y ValI 230 li1e Ph e Al a Al a Ser 310 ValI O31n Al a I Ie Thr 390 Al a Tyr Hi s Pr o Thr 135 Al a Ar g Ser Al a Le u 215 ValI I Ie Pr o Thr Ar g 295 As p Hi s Al a ValI O31y 375 O31u As p Pr o O31y 1 05 As p O31y Oy le Tyr 1 85 ValI Al a Ser Al a O31n 265 Le u Le u Ly s ValI As p 345 Ph e Pr a Al a O31u Tyr 425 Al a Ly s vb t ValI Al a 1 70 Pr a O31y Al a Thr Ly s 250 O31n Ly s O31u Al a Le u 330 Le u As p Al a As p Ser 410 O31u O31n I Ie Ly s As p 1 Le u Ar g Al a O31y ValI 235 O31n O31y I Ie O31y Al a 315 Al a Le u Pr a Le u I Ie 395 Le u O31y Al a vb t Le u 140 Pr a Ly s Hi s Ly s Le u 220 Hi s O31u O31y Al a Al a 300 O31y As p Hi s Ar g Al a 380 li1e Ar g Le u As n O31y 125 As n O31u O31u Le u Le u 205 Hi s Ly s Tyr Pr a O31y 285 Ar g ValI Le u O31u Pr a 365 Thr O31y O31y O31u Al a 110 Le u Ph e Thr O31n As p 1 Tr p Pr a Thr Al a Le u 270 Thr I Ie As p Ar g ValI 350 Pr a Ar g Thr Ar g As p 430 Al a Ser Ser vb t Pr a 1 Ph e ValI Ser Le u Ly s 255 vb t O31u Le u ValI As n 335 O31y vb t O31y Al a ValI 415 Tr p Page 196 seqI i st car r ect ed. t xt <210O> 145 <21 1> 401 <21 2> DNA <21 3> COr ynebact er i urn gi ut amr cumn <220> <221 CIDS <222> (378) <223> RXA01821 <400> 145 cga Ar g ac c Th r g aa (31u ag c Ser gt g ValI aac As n gca A a t gg Tr p ag c Ser at c I Ie ac a Th r ct c Le u ac c Th r cca Pr o t gc CYS gt t ValI 115 ggC G31y g ac As p gt g ValI ccc Pr o tt c Ph e tt c Ph e g at As p tt a Le u aaa Ly s aac As n gac As p t cc Ser ct g Le u 70 at c I Ie ggg G31y ag c Ser t gg Tr p ggc G31y cgc Ar g at c I Ie CCC Pr o cgt Ar g t ac Ty r ct c Le u cca Pr o c ac Hi s at c I Ie cca Pr a aat As n cgt Ar g at c I Ie 1 05 cca Pr a agt Ser 10 gt a ValI gt c ValI ct g Le u cca Pr a g at As p 90 aaa Ly s ccg Pr a ac g Th r gt c ValI ac c Th r ctt Le u gt g ValI 75 ggC G31y at t I Ie at g vb t cga Ar g at c I Ie ac c Th r g aa (31u g ac As p tt c Ph e gcc A a t gc Cys t ca Ser c aa O31n G31y gt g ValI tt c Ph e c ac Hi s gga G31y cct Pr a 125 cca Pr a gca A a ag c Ser cga Ar g c aa O31n gt a ValI tt g Le u 110 cgg Ar g aaa Ly s ggc G31y t gg Tr p cgc Ar g ccg Pr a tt t Ph e g at As p aat As n g aa O31u ac a Th r ct a Le u g aa O31u gga G31y gag O31u t gat gt cct g at cccggt tc cgg <210O> 146 <211> 126 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 146 Arg Asn Ser On Gy Lys Trp Cys Pro 1 5 Thr Ser Il e 0 u Asp Asn 0 y Asp Hi s 25 O31u Thr Thr I Ie Val Asp Ar g Val I Ie 40 Ser O31u Leu Val Pro Ser IlieAla Pr o 55 Val Leu Thr Tr p Phe Leu Pro Asn Asn 70 Ser Thr Arg Ser Pro Lys Asn 10 Val Val Ilie On Ala G1y Ou Val Thr Thr G31y Se r Trp Thr Leu Leu 03 u Val Ar g Arg Leu Pr o Val As p Phe (31n Pro (31u 75 Page 197 seqi i st car r ect ed. t xt Asn Leu Pro Cys Phe lie Arg Asp Arg Asp Gly Phe His Val Phe G1y 90 Ala Pro Cys Val Asp G31y Ty r Se r I Ie Lys IlieAla Gy Leu Asp Ou 100 105 110 Trp G31y Val Pr o Leu Ser Leu Asp Pr o Pr o l'bt Cys Pr o Ar g 115 120 125 <210O> 147 <21 1> 488 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> 101). (478) <223> RXN02263 <400> 147 cct gggcaac ccaagt gt at gaaaacgccc t ggaaaaagg tgtgggaatc acccgcactg gcttgagaga agaaacaaca cgtcggcacc acattgaacc at c I Ie at c G31y c ac Hi s t gg Tr p ggt G31y gt g ValI gcg Al a ct t Le u ggt G31y G31y ggc G31y t ca Ser tt a Le u t ca Ser at g vb t 120 t ca Ser gag (31u tt c Ph e ac c Th r ag c Ser ac c Th r g aa (31u acc Th r gcc Al a aca Th r t ac Ty r gag (31u 75 ggc G31y cgt Ar g ggc G31y at c I Ie ggg G31y gt t ValI at t I Ie aag Lys5 t at Ty r at g vb t tt t Ph e t cc Ser tt g Le u gga G31y g ac As p ct g Le u 110 gca Al a 15 g aa (31u cga Ar g ct c Le u cgc Ar g g aa (31u 95 cca Pr o ct g Le u c aa O31n ct g Le u aaa Lys5 g aa G31u 80 gca Al a cat Hi s at g vb t 1 t gg Tr p tt c Ph e tt t Ph e cgc Ar g ct c Le u ccc Pr o g aa (31u aaa at t gcg Lys I I e Al a c ac Hi s ggc G31y cgc Ar g gca Al a tt c Ph e tt c Ph e cga Ar g tt a Le u at c I Ie at g vb t cga Ar g c ac Hi s c aa O31n ct t Le u 115 agt Ser t cc Ser gcc Al a gca Al a aac As n cgc Ar g 1 00 ac c Thr gt a ValI aac As n cat Hi s t ac Tyr ct a Le u tt c Ph e ct g Le u gcc Al a cgc agc gt t acc Ar g Ser Val Thr cag gt C 03 n Val1 125 t agact t ccg <210O> 148 <211> 126 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 148 l'bt Lys I Ie Al a Val I Ie G31y Leu G31y Ser Thr G31y Ser l'bt Al a Leu Page 198 Tr p Ph e Ph e Ar g Le u Pr o O31u Le u lIe vb t Ar g Hi s O31n Le u 115 As n Hi s Tyr Le u Ph e Le u A a Pr o Tyr O31u Ser ValI O31u O31n seql i st car r ect ed. t xt 10 G31y Va 1O31u Ala I Ie G31y O3 y A a Phe Thr 03 y 03 u 40 G31y Ser Thr Ty r Val Pr o Ser Leu Ser O31u I Ie Ser 75 Leu Ser Thr 03 y Lys 03 u 90 Ser Val O31u Arg Tyr O31u 1 [Vb t Ar g Ser Val Thr O31n 120 125 O31u Ar g Le u Ar g O31u Pr o O31n Le u Ly s O31u A a Hi s <210O> 149 <21 1> 460 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (460) <223> FRXA02263 <400> 149 cct gggcaac ccaagt gt at gaaaacgccc t ggaaaaagg tgtgggaatc acccgcactg gcttgagaga agaaacaaca cgtcggcacc acattgaacc at c I Ie at c G31y c ac Hi s t gg Tr p ggt G31y gt g ValI ct t Le u ggt G31y ggc G31y ggc G31y t ca Ser tt a Le u t ca Ser t ca Ser gag O31u tt c Ph e ac c Thr ag c Ser ac c Thr g aa (31u acc Thr gcc A a aca Thr t ac Tyr gag O31u 75 ggc G31y cgt Ar g ggc G31y at c I Ie ggg G31y gt t ValI at t I Ie aag Ly s t at Tyr t cc Ser ggc G31y gag O31u 45 ccg Pr o t cc Ser g aa O31u gag (31u at g vb t tt t Ph e t cc Ser tt g Le u gga G31y g ac As p ct g Le u 110 gca A a 15 g aa O31u cga Ar g ct c Le u cgc Ar g g aa O31u 95 cca Pr o ct g Le u c aa O31n ct g Le u aaa Ly s g aa O31u 80 gca A a cat Hi s at g vb t 1 t gg Tr p tt c Ph e tt t Ph e cgc Ar g ct c Le u ccc Pr a g aa (31u aaa Ly s c ac Hi s ggc G31y cgc Ar g gca A a tt c Ph e tt c Ph e cga Ar g at t I Ie tt a Le u at c I Ie at g vb t cga Ar g c ac Hi s c aa O31n ct t Le u 115 gcg A a agt Ser t cc Ser gcc A a gca A a aac As n cgc Ar g 1 00 ac c Thr gt a ValI aac As n cat Hi s t ac Tyr ct a Le u tt c Ph e ct g Le u gcc A a Page 199 seqI i st car r ect ed. t xt gcg cag at g A a G3 n [Vb t 120 <210> 150 <211> 120 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri curn <400> 150 I'bt Lys Ilie Ala Val Ilie Gy Leu Gy 1 5 Tr p Hi s Leu Ser Asn Ilie ProGy Val Phe G31y I Ie Ser Hi s G31y Ty r G31y Ala 40 Phe Ar g I'bt Ala Tyr Hi s 31u G31y Se r Ar g Ala Ar g Ala Leu Tr p Ser Ser Leu 70 Leu Phe Hi s Asn Phe G3 y ValI Leu Ser Pr o Phe O31n Ar g Leu Va 1O31u Ser Val 100 105 O31u Ar g Leu Thr Ala Ala O31n Nibt 115 120 Th r A a Th r Ty r O31u G31y Ar g Ser G31y O31u Pr o Ser O31u O31u A a O31u Ar g Le u Ar g O31u Pr o <210O> 151 <21 1> 1251 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (1228) <223> RXA02176 <400> 151 gggt gct agg aact gacagc t tcagggt ta t agt tgt tgg t ggccct t tt act tccaagc gcagaaagt t gcccgaagac ac c Thr cct Pr o gca A a gt g ValI ccc Pr o aag Ly s gt c ValI t ca Ser gag O31u cga Ar g ggt G31y cgc Ar g t t c Ph e cca Pr a acc Thr gag O31u at c I Ie g aa O31u t ca Ser gga G31y ggC G31y at t lIe cgt Ar g t cc Ser gac ggc As p (1y 15 cag gct (3 n A a cag ccg O31n Pr o gac ct C Asp Leu Page gt cagat cgt t aacgat ccc at g acc gac ttc ccc I'b t Thr Asp Phe Pr o 1 cgt t tc ggc t gc gga Ar g Phe G31y Cys G31y at t gt c gac gga t cc Ilie Val Asp G31y Ser gca gta aaa aac gtc A a Val Lys Asn Val ttc tcc ctt cca gaa Phe Ser Leu Pr o O31u seql i st cor r ect ed. t xt ggc Gy gca Al a ggt Gy ct c Leu cca Pr o acc Thr 150 ggc Gy gt a Val ttc Phe ct c Leu ttc Phe 230 t ac Tyr aag Lys aca Thr gca Al a acc Thr 310 ct g Leu gag Gu acc Thr ttc Phe gag G u 120 gca Al a act Thr ct g Leu at c SIle t cc Ser 200 cgc Ar g aac Asn acc Thr at g Met agc Ser 280 cca Pr o gac Asp gca Al a at c SIle gga Gy t cc Ser gag G u gaa G u gcc Al a gcc Al a 170 aac Asn ggt Gy gag G u cag G n gct Al a 250 t cc Ser t cc Ser gt g Val gat Asp ggc Gy ctt Leu 75 ct c Leu aag Lys at c SIle ggc Gy at g bet 155 at t l Ie t cc Ser ggc Gy aag Lys acc Thr 235 gtt Val aac Asn gcc Al a gca Al a gac Asp 315 at c SIle t cc Ser at t SIle ttc Phe gt c Val gcc Al a 140 gtt Val gac Asp gat Asp ct g Leu at c SIle 220 gca Al a gct Al a ggc Gy ct g Leu gat Asp 300 t cc Ser ct g Leu ct a ggt ggt Leu G y G y gaa aag aag G u Lys Lys 95 gca aag gct Al a Lys Al a 110 acc gca gaa Thr Al a G u 125 gat gtt at t Asp Val I le cca gtt ct t Pro Val Leu gca acc t cc Al a Thr Ser 175 gtt tac tac Val Tyr Tyr 190 tgg ctt gca Trp Leu Ala 205 aac gct t cc Asn Al a Ser gtg gat aac Val Asp Asn acc ttg ctg Thr Leu Leu 255 ggc ctg gat G y Leu Asp 270 t ac aac t gg Tyr Asn Trp 285 gca gct aag Al a Al a Lys atc gac gca I e Asp Al a gac acc gag Asp Thr G u gcg Al a 80 t cc Ser t ct Ser acc Thr gca Al a cgc Ar g 160 ggc Gy ttc Phe gcg Al a gat Asp t cc Ser 240 at g Met gga Gy gct Al a cgc Ar g gca Al a 320 gca Al a cac Hi s ctt Leu gac Asp 130 gca Al a gaa G u ggt Gy cca Pr o agc Ser 210 ttc Phe aag Lys gac Asp gtt Val gct Al a 290 ct c Leu at c l Ie ttc Phe ctt Leu gct Al a 115 t ct Ser cac Hi s ggc Gy gga Gy cag G n 195 cca Pr o at c SIle aac Asn aac Asn gct Al a 275 cgc Ar g gtt Val gct Al a tgg Tr p t ct Ser 100 cct Pr o ccg Pr o aac Asn tct Ser ct g Leu 180 aag Lys gca Al a cct Pr o cag G n cag G n 260 cgc Ar g gag G u gt c Val aag Lys gat Asp ttc Phe tgg Tr p gcc Al a gaa G u gaa G u 165 cca Pr o tgc Cys gct Al a gag G u acc Thr 245 gt c Val acc Thr gag G u ggc Gy at a SIe 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 cct tac cgc aag ctg gga Pro Tyr Arg Lys Leu Gy Page 201 seqI i st car r ect ed. t xt 330 335 340 cgc aac cag ct g cgc at c ggt at g t tc cca gcg at c gat t cc acc gat Ar g As n O31n Leu Ar g I Ie O31y I'bt Phe Pr o Ala I Ie Asp Ser Thr Asp 345 350 355 gt ggaa aag ct cacc gga gca at cgact t cat cct cgat ggc ggt tt t Val O31u Lys Leu Thr G31y Ala Ilie Asp Phe Ilie Leu Asp G31y G31y Phe 360 365 370 gca agg aag taataccccc actttgaaaa aca Ala Ar g Lys 375 <210> 152 <211> 376 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1171 1219 1251 <400> 152 I'bt Thr Asp Phe Pro Thr Ar g A a Ph e Thr G31y Ly s G31y Tr p 145 Pr o A a Ser vb t Ar g 225 Le u G31y As p Ly s Le u Ph e Le u A a 115 Ser Hi s G31y G31y O31n 1 95 Pr o I Ie As n G31y Ser ValI O31u As p Ph e Tr p A a O31u O31u 1 65 Pr o cys A a O31u Thr 245 Pr o A a ValI G31y 70 A a G31y Le u Pr a Thr 150 G31y ValI Ph e Le u Ph e 230 Tyr Leu Pro Ser Lys Ser Val 40 O3 y Ser Ty r 03 u A a Thr O3 u Phe As p (1u 120 O3 n A a 135 Ser Thr Ser Leu As p II e A a Ser 200 O31u Ar g 215 Leu Asn Asn Thr Ser 03 u Phe 10 Val Arg Pro 1 1 e 03 y Thr I Ie Ar g O31u I I e I I e Leu 75 Phe 03 y Leu 90 Ser Ser Lys 1 05 Pr o O31u IIe Phe 03 u 03 y 03 y A a [ib t 1 55 Val Ala I Ie 1 70 Lys Asn Ser 1 85 Asp (1y (1y Ile 03 u Lys Leu 03 n Thr 235 Pr o Ala Val 250 I Ie O31u Ser G31y Ser I Ie Ph e ValI A a 140 ValI As p As p Le u I Ie 220 A a Pr a O31n Hi s Le u Le u O31u A a Thr 125 As p Pr a A a ValI Tr p 205 As n ValI Gy le Ar g Ser G31y Ly s Ly s 110 A a ValI ValI Thr Tyr 1 Le u A a As p As p O31n O31n As p O31y Ly s A a Ou le Le u Ser 1 Tyr A a Ser As n O31y A a Pr a Le u A a Ser Ser Thr A a Ar g 1 O31y Ph e A a As p Se r 240 A a Thr Leu Leu N/t 255 Page 202 seql i st cor r ec Leu Asp Asn G n Val Lys Trp IVet Asn Ser Asn 260 265 lMt Val Ala Arg Thr Thr Ala Ser Ser Ser Ala 275 280 G u Ala Arg G u G u Al a Ser Pro Tyr Val Ala 290 295 Ser Leu Val Val y Thr Ile Asp Phe Asp Asp 305 310 315 Val I e Ala Lys Ile Leu Arg Ala Asn y lile 325 330 Tyr Arg Lys Leu y Arg Asn G n Leu Arg lie 340 345 I e Asp Ser Thr Asp Val G u Lys Leu Thr y 355 360 Leu Asp y y Phe Al a Arg Lys 370 375 <210> 153 <211> 1422 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1399) <223> RXN02758 <400> 153 atacatctca cccaattccc cataactaga caattgccca aatgtcgtgt tccgcgctca gacatgagac aattgttgcc ted. txt G y G y Leu Tyr 285 Asp Al a 300 Ser Ile Leu Asp G y let Ala I e 365 Leu 270 Asn Al a Asp Thr Phe 350 Asp Asp Tr p Lys Al a G u 335 Pr o Phe gcaacgactg ataagtctcc gtg act Val Thr gaa ct c at c G u Leu I I e cag Gn gca Al a gac Asp cag G n aac Asn acc Thr gaa Gu cgt Ar g cca Pr o cag G n gcg Al a ggc G y caa Gn ggt G y gt g Val ttg Leu ttt Phe act Thr gaa Gu t at Tyr act Thr gac Asp gt g Val 75 gac Asp at c SIle ctt Leu gcc Al a gtt Val ggt G y acc Thr gct Al a cct Pr o gcg Al a 45 gag G u at c SIle ct c Leu gag Gu gcg Al a ttc Phe cag G n gca Al a aag Lys gaa Gu at c l Ie agg Ar g at g Met gag G u 80 cat Hi s 1 gcc Al a aca Thr gt c Val ttc Phe cgt Ar g gga G y ggc Gy gt g Val ttg Leu cgt Ar g gt c Val cag G n cag Gn ggt G y gct Al a ttt Phe acc Thr gt g Val 100 gtt Val aaa Lys aat Asn ttg Leu gtc Val gtg Val gtg gag ctg cag gaa act gtg cag tcg tcc cgt cct cgt t ct t cc cat Page 203 seql i st cor r ect ed. t xt Val u Leu n u Thr Val n Ser Ser Arg Pro Arg Ser Ser Hi s 115 105 gtt Val at t SIle ggt Gy 150 ccg Pr o gct Al a ttg Leu ttg Leu gaa G u 230 gat Asp gat Asp ggt Sy gct Al a gag G u 310 ggc Gy aag Lys t ac Tyr gt g Val 120 cag G n tcg Ser gt c Val acc Thr cgt Ar g 200 act Thr gaa G u gag G u tcg Ser cgc Ar g 280 gtt Val gag G u ct g Leu gag G u act Thr 360 ttg Leu ct g Leu t ac Tyr cct Pr o 170 gag G u tct Ser gag G u gcg Al a tct Ser 250 at c SIle acg Thr ggt Gy gat Asp ggc Gy 330 ct c Leu gct Al a ggt G y gcg Al a cct Pr o 155 ggt Gy ct g Leu aag Lys gt c Val gca Al a 235 ct g Leu gat Asp at c SIle ggt Gy t at Ty r 315 aac Asn cgt Ar g gt c Val gat Asp gat Asp 140 gt g Val ggt Gy aat Asn cgt Ar g at t SIle 220 gtt Val cgt Ar g gag G u cgt Ar g ttc Phe 300 gt c Val gt c Val gag gtu ggt Gy 110 gtt Val gat Asp ggc Gy gaa G u gat Asp 190 gt g Val at g Met gag G u cgt Ar g gct Al a 270 ct g Leu cag G n gcc Al a gga Gy gct Al a 350 ggc Gy gat Asp gcc Al a ct g Leu gcg Al a 175 at t SIle tgc Cys ct g Leu cgt Ar g gt g Val 255 gcc Al a aac Asn gt g Val aac Asn aag Lys 335 gcg Al a gct Al a gcg Al a aac Asn gag G u 160 at g let gcg Al a ttc Phe gcg Al a gcg Al a 240 aag Lys gct Al a cgc Ar g ttg Leu act Thr 320 at c SIle gat Asp aat Asn ttg Leu at t SIle 145 ct g Leu cgt Ar g at t l Ie gat Asp gct Al a 225 at g let gcg Al a at t l Ie at g Met gaa G u 305 ttg Leu gtt Val tct Ser gac Asp gat Asp 130 gac Asp aag Lys aag Lys gag G u tgt Cys 210 cac Hi s cgc Ar g ttg Leu gag G u ggt G y 290 ggt Gy gaa G u gac Asp ggc Gy at c SIle 370 at t SIle acc Thr gt g Val gcg Al a cgt Ar g 195 gat Asp gcg Al a ggt G y gct Al a ct g Leu 275 t ac Tyr ttg Leu at c SIle cgc Ar g ct g Leu 355 gat Asp t cc Ser at t SIe act Thr ctt Leu 180 tct Ser t cc Ser ggc G y gag G u ggt G y 260 acc Thr cag G n gct Al a gtt Val gct Al a 340 aag Lys at g let 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 tcc gct gcg ggt ctg ggt gtt gct ttc aac gcg aag cct gcg ctg aag Page 204 seqI i st car r ect ed. t xt Ser Al a Al a (31y L eu G31y Val Ala Phe Asn Ala Lys Pr o Al a Leu Lys 375 380 385 gag at t gcg gat act t cc gt gaac cac ccat t cct cgac gag gt t tt g O31u Ilie Al a Asp Thr Ser Val Asn Hi s Pr o Phe Leu Asp O31u Val Leu 390 395 400 405 cac at cat gggc at t t cc cgc gac gag at cgat ct ggcg gat cag gaa His Ilie b t G31y Ilie Ser Ar g Asp O31u Ilie Asp Leu Al a Asp O31n O31u 410 415 420 gac ggc act t tc cac cgc gt t cca t tg acc aat gcc t aaagat tcg Asp G31y Thr Phe Hi s Ar g Val Pr o Leu Thr Asn Al a 425 430 tttctcgacg ccc <210> 154 <211> 433 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1315 1363 1409 1422 <400> 154 ValI Al a Thr ValI Ph e Ar g G31y Pr o Le u I Ie 145 Le u Ar g I Ie As p Al a 225 Thr 03 u Leu 31y O31n O31n Val Ser G31y Leu Ser Al a Ar g G31y Phe Val O31u Thr 03 n Ser Val 1 00 Ar g Ser Ser 115 As p II e Ser 130 As p Thr I Ie Lys Val Thr Lys Al a Leu 1 80 O31u Ar g Ser 1 95 Cys Asp Ser 210 Hi s Al a 03 v le ValI Lys5 As n Le u ValI ValI Hi s Ar g Ar g ValI 1 65 Al a G31y Thr Lys5 31n As n Al a Leu Asp Arg 03 n Val 55 Asn Leu 70 Thr Thr Val O31u Val Val I Ie G31y 135 G31y I Ie 150 Pr o As p Al a Leu Leu Leu Leu I I e 215 03 u Al a 230 O31u Ar g Pr o 40 O31n Al a G31y Le u ValI 120 O31n Ser ValI Thr Ar g 200 Thr (31u Ser O31u 25 G31y ValI Al a Le u O31n 1 05 ValI Thr As p Ser Ser 1 85 Ar g O31y ValI O31u Tyr Thr As p ValI 75 As p Thr O31y Al a Pr o 1 55 O31y Le u Lys5 ValI I Ie Le u Al a ValI O31y Thr ValI As p As p 140 ValI O31y As n Ar g I Ie 220 Al a Pr o Al a O31u I Ie Le u O31n Pr a 125 Tyr Thr O31y ValI Le u 205 O31u O31u Al a Ph e O31n Al a Lys5 Ser 110 ValI As p O31y O31u As p 1 ValI vb t Le u ValI Ph e Ser Pr a ValI Ser As p Al a Le u Al a 1 li1e cys Le u Ou le Ar g vb t O31u Hi s Ar g Al a As n O31u 1 vb t Al a Ph e Al a Al a 240 Al a Al a ValI Th r (31u Ar g 235 Page 205 seql i st cor r ect ed. t xt Ibet Arg y G u Leu Asp Phe G u G u Ser 245 250 Leu Arg G u Arg Val Lys 255 Al a SIle et G u 305 Leu Val Ser Asp Lys 385 Leu Leu Al a Al a Leu 275 Tyr Leu SIle Ar g Leu 355 Asp Al a G u Asp Leu Pr o Thr G u Asp 325 Al a bet Leu Lys Leu 405 G u Al a Al a Val 295 Leu Lys Al a G n Al a 375 I le SIle Gy Ser Ar g 280 Val G u Leu G u Thr 360 Al a Al a Met Thr Val 265 Thr Ser Leu Thr Phe 345 Val Gy Asp Gy Phe 425 Asp SIle Gy Tyr 315 Asn Ar g Val Gy Ser 395 Ser Ar g Val Thr 285 I le Ar g Thr Phe Asp 365 Al a Asn Asp Pr o Al a 270 Leu G n Al a Gy Al a 350 Gy Phe Hi s G u Leu 430 Al a Asn Val Asn Lys 335 Al a Al a Asn Pr o SIle 415 Thr Al a Ar g Leu Thr 320 I e Asp Asn Al a Phe 400 Asp Asn <210> 155 <211> 490 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(490) <223> FRXA02479 <400> 155 atacatctca cccaattccc cataactaga caattgccca gcaacgactg ataagtctcc aatgtcgtgt tccgcgctca gacatgagac aat t gt t gcc gtg act gaa ctc atc Val Thr G u Leu Ile 1 cag aat gaa tcc caa gaa atc gct gag ctg gaa gcc ggc cag cag gtt Sn Asn u Ser n u I e Al a u Leu u Al a y n n Val 15 gca ttg cgt gaa ggt tat ctt cct gcg gtg atc aca gtg agc ggt aaa Ala Leu Arg G u y Tyr Leu Pro Ala Val lie Thr Val Ser y Lys 30 gac cgc cca ggt gtg act gcc gcg ttc ttt agg gtc ttg tcc gct aat Asp Arg Pro y Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala Asn Page 206 cag G n aac Asn acc Thr gt g Val gtt Val cag G n gcg Al a ggc Gy ct g Leu gt g Val 120 ttg Leu ttt Phe act Thr gaa G u ttg Leu gtt Val ggt Gy acc Thr gt g Val gat Asp seql i st cor r ect ed. t xt 45 gag cag tca atg ttc cgt G u G n Ser IVet Phe Arg atc gca cct gag cgt gtc lie Ala Pro u Arg Val 80 ctc aag gtg cat gga cag Leu Lys Val His G y G n 95 cag tcg tcc cgt cct cgt G n Ser Ser Arg Pro Arg 110 ccg gtt gat gcg ctg gat Pro Val Asp Ala Leu Asp 125 130 ttt ttg Phe Leu acc gtc Thr Val gtg gtg Val Val 100 t cc cat Ser His <210> 156 <211> 130 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 156 Val Thr u Leu Ile On Asn Ou Ser 1 5 Al a Gy On On Val Ala Leu Arg u 25 Thr Val Ser y Lys Asp Arg Pro Gy 40 Val Leu Ser Ala Asn G n Val G n Val 55 Phe Arg y Phe Leu Asn Leu Ala Ala 70 Arg Val G u Thr Val Thr Thr y Leu Sy G n Ser Val Val Val G u Leu G n 100 105 Pro Arg Ser Ser His Val Val Val Val 115 120 Leu Asp 130 <210> 157 <211> 558 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(535) <223> FRXA02758 <400> 157 G u Tyr Thr Asp Val Asp Thr Sy SIle Leu Al a Val Sy Thr Val Asp Al a Pr o Al a G u SIle Leu G n Pr o 125 G u Al a Phe G n Al a Lys Ser 110 Val Leu Val Phe Ser Pr o Val Ser Asp Page 207 seql i st cor r ect ed. t xt aaggcgttgg ctggtttgga tgcgtcggtg atcgatgagg tcgctgccgc tattgagctg acccttggtg cgcgcaccac gatccgtacg gttgaaccgc atg ggt tac cag acc Met y Tyr G n Thr gct gtt gtt tcc Ala Val Val Ser gag ttg gag ttg G u Leu G u Leu ggc aag ctg acc G y Lys Leu Thr aag gct gag ttc Lys Ala G u Phe tac cag act gtc Tyr G n Thr Val tcc gct gcg ggt Ser Al a Al a G y gag att gcg gat G u Ile Ala Asp 105 cac atc atg ggc His lie Iet G y 120 gac ggc act ttc Asp y Thr Phe 135 tttctcgacg ccc <210> 158 <211> 145 <212> PRT <213> Corynebact <400> 158 'e t y Tyr n 1 G u G y Leu Al a Leu G u I e Val Val Asp Arg Ala Ser G y Leu Lys Asp I e Asp I\et ggt Gy gat Asp ggc Gy ct c Leu gct Al a ct g Leu act Thr at t SIle ttc Phe gt c Val gt c Val gag G u 60 ggt Gy gtt Val gt g Val cgc Ar g cag G n gcc Al a gga Gy gct Al a ggc Gy ttc Phe cac Hi s 110 gag G u gt g Val 15 aac Asn aag Lys gcg Al a gct Al a aac Asn 95 cca Pr o at c SIle ttg Leu act Thr at c SIle gat Asp aat Asn gcg Al a ttc Phe gat Asp 1 gaa G u ttg Leu gtt Val tct Ser gac Asp aag Lys ct c Leu ct g Leu ggt Gy gaa G u gac Asp ggc Gy at c SIle cct Pr o gac Asp gcg Al a 130 gct Al a gtt Val gct Al a aag Lys at g Met ctg Leu 100 gtt Val cag G n cac cgc gtt His Arg Val cca ttg acc aat gcc Pro Leu Thr Asn Ala t aaagatt cg er i um gl ut ari cum Thr Al a Val Val Ser 5 G u G u Leu G u Leu 25 Asp G y Lys Leu Thr 40 Ala Lys Ala G u Phe bet Tyr G n Thr Val 70 Leu Ser Al a Al a G y G y G yI Asp Tyr Sy Asn Leu Arg Ala Val Leu G y Page 208 SIle Ar g Thr Phe Asp Al a G n Al a Sy Al a Sy Phe seql i st cor r ect ed. t xt Lys Pro Ala Leu Lys G u Ile Ala Asp Thr Ser Val Asn His Pro Phe 100 105 110 Leu Asp G u Val Leu His lie Iet y I I e Ser Arg Asp u I I e Asp 115 120 125 Leu Ala Asp G n G u Asp G y Thr Phe His Arg Val Pro Leu Thr Asn 130 135 140 Al a 145 <210> 159 <211> 205 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (72)..(182) <223> FRXA02759 <400> 159 tcttacttct aagctgaatg tgatattgcg attaagcgtt ctggtttgct gcgtcgttct aagcgtctgg t gtg ctt cga ttg tat ccc acg ttg atc act ggt gag gtc 110 Val Leu Arg Leu Tyr Pro Thr Leu Ile Thr y G u Val 1 5 att gag atg ctg gcg gct cac gcg ggc aag gaa gct aaa gtt gcg gca 158 lie u Iet Leu Ala Ala His Ala Gy Lys Gu Ala Lys Val Ala Ala 20 gtt act gag cgt gcg atg cgc ggg tgagctcgat ttcgaggagt ctc 205 Val Thr G u Arg Al a IVbt Arg G y <210> 160 <211> 37 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 160 Val Leu Arg Leu Tyr Pro Thr Leu Ile Thr Gy u Val Ile Gu IVet 1 5 10 Leu Ala Ala His Ala y Lys u Ala Lys Val Ala Ala Val Thr G u 25 Arg Ala IVet Arg Gy <210> 161 <211> 1188 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1165) <223> RXA02501 <400> 161 cgccccgaaa gctttaccat ggatacgata ctcaatggag atatccatta tcggtttggc Page 209 seqI i st car r ect ed. t xt gt ctgt gcgc t cat gaacca ggaaggt t tt t gt t tgt ctc at g agc t ct gaa gga 115 I'b t Ser Ser O31u G31y 1 aga aac cac aac tgg gac tac gcc gcc atc ggc acc cca gag gat ttc 163 Arg Asn His Asn Tr pAsp Tyr Ala Ala I Ie G31y Th r Pr o O31u Asp Phe 15 ct c gcc agc t gg agc gca tcc cgc gga aat cta cga cgc t tt t tc gaa 211 Leu Al a Ser Tr p Ser Al a Se r Ar g G31y Asn Leu Arg Ar g Phe Phe O31u 30 gac cac gca gcc gcc ccc ata aac gat gcc gcc cag cgc caa gca ggt 259 Asp Hi s Al a Al a Al a Pro I Ie Asn Asp Al a Al a O31n Ar g O31n Al a O31y 45 gaa gcc gca gca acc caa gcc gt c gca gcg at c t ac ggc at g gag ct C 307 O31u Al a Al a Al a Thr O31n Al a Val Al a Al a Ilie Tyr G31y 'b t O31u Leu 60 aac gaa ttc aac gca ggt gtc gac gcc gtc gcc ggc gcc atc gaa tct 355 Asn O31u Phe Asn Al a O31y Val Asp Al a Val Al a O31y Al a Ilie O31u Ser 75 80 gcc ggc gcc at c cac gt c agc at c ccc gat ccc gat gtc CCCC caa gat 403 Ala O31y Al a Ilie Hi s Val Ser Ilie Pr o Asp Pr o Asp Val Pr o O31n Asp 95 100 gtc gga gcc gca gca ttt ttc gac gtc gac aac acc ctc atc caa ggc 451 Val O31y Al a Al a Al a Phe Phe Asp Val Asp Asn Thr Leu Ilie O31n O31y 105 110 115 t cc t cc ct c at c gt t t tc gcc caa gga ct c t tc cgg aag aaa t tc t tc 499 Ser Ser Leu Ilie Val Phe Al a O31n O31y Leu Phe Ar g Lys Lys Phe Phe 120 125 130 acc at c aaa gaa at c ct c ccc gt g gt g t gg aaa caa gt g aaa t tc aaa 547 Thr Ilie Lys O31u Ilie Leu Pr o Val Val Tr p Lys O31n Val Lys Phe Lys 135 140 145 ct C acc ggc t cc gaa aac gcc gac gac gt c t cc cgc ggc cgc gaa caa 595 Leu Thr 31y Ser 31u Asn Ala AspAsp Val Ser Ar g 1y Ar g (1u (1n 150 155 160 165 gcc ct cgaat t cat caaa ggc cgc ccc gt ccaa gaa ct agtt gac ct c 643 Al a Leu O31u Phe Ilie Lys O31y Ar g Pr o Val O31n O31u Leu Val Asp Leu 170 175 180 tgc gaa gaa atc gtc gac caa cgc atg gcc gac aaa atg tgg ccc ggc 691 Cys (1u (1u Ilie Val Asp (1n Ar g /b t AiaAsp Lys I'b t Tr pPr o (1y 185 190 195 acc aaa caa ct C gcc gac at g cac at c gcc gcc ggc cac caa gt C t gg 739 Thr Lys O31n Leu AiaAsp 'b t Hi s IleAlia Alia 1y Hi s O31nVal Tr p 200 205 210 ctc gtc tcc gca acc ccc gtc caa ctc gcc caa atc ctg gca caa cgc 787 Leu Val Ser Al a Thr Pr o Val O31n Leu Al a O31n Ilie Leu Al a O31n Ar g 215 220 225 ctc ggc ttc acc gga gcg atc ggc aca gtc gca gaa gca aaa gat gga 835 Leu O31y Phe Thr O31y Al a Ilie O31y Thr Val Al a O31u Al a Lys Asp O31y 230 235 240 245 gta ttc acc ggc cga ct C gtc ggc gac atc ctc cac gga ccc ggc aaa 883 Val Phe Thr (3y Ar g Leu Val (3y Asp Ilie Leu Hi s (3y Pr o (3y Lys 250 255 260 Page 210 seql i aga cac gca gtc gca gca ctc gca tcc Arg His Ala Val Ala Ala Leu Ala Ser 265 270 cga tgc acc gcc tac tcc gac tcc atc Arg Cys Thr Ala Tyr Ser Asp Ser Ile 280 285 atg gtc ggc acc gcc gtc gca gta aac Met Val G y Thr Ala Val Ala Val Asn 295 300 gaa gcc gaa acc cga ggc tgg gac gtc G u Ala G u Thr Arg G y Trp Asp Val 310 315 aaa gcc acc cgc gaa tac gga atc ccc Lys Ala Thr Arg G u Tyr Gy lie Pro 330 agt gtc gcc ggc tgg agt cta cgc cgc Ser Val Ala y Trp Ser Leu Arg Arg 345 350 taacgcacag gagccgtttt aag <210> 162 <211> 355 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 162 IVet Ser Ser G u G y Arg Asn His Asn 1 5 Thr Pro G u Asp Phe Leu Ala Ser Trp 25 Arg Arg Phe Phe u Asp His Ala Ala 40 G n Arg G n Ala G y G u Ala Ala Ala 55 Tyr G y IVet G u Leu Asn G u Phe Asn G y Al a I e u Ser Al a y Ala I le Asp Val Pro G n Asp Val y Ala Al a 100 105 Thr Leu lie n y Ser Ser Leu Ile 115 120 Arg Lys Lys Phe Phe Thr Ile Lys G u 130 135 G n Val Lys Phe Lys Leu Thr G y Ser 145 150 Arg y Arg G u n Al a Leu G u Phe 165 st cor r ect ed. t xt at c gaa caa ct c lie G u G n Leu aac gac ctc ccc Asn Asp Leu Pro 290 ccc gac tcc aaa Pro Asp Ser Lys 305 cgc gat ttc cga Arg Asp Phe Arg 320 gcc ctg gtc acc Ala Leu Val Thr 335 cga tgg aga aaa Arg Trp Arg Lys ct c Leu ct c Leu cgc Ar g at c SIe gca Al a 340 931 979 1027 1075 1123 1165 1188 Tr p 10 Ser Al a Thr Al a Hi s 90 Al a Val SIle Su I e 170 Tyr Ser l Ie Al a Val Ser Phe Al a Pr o 140 Al a Sy Al a Ar g Asn Val Asp SIle Asp G n 125 Val Asp Ar g Al a Sy Asp Al a Al a Pr o Val 110 Sy Val Asp Pr o I e Asn Al a Al a Val Asp Asp Leu Tr p Val Val 175 G u Leu Val Asp Leu Cys Gu u G ule Val Asp n Arg IVet Ala Asp Page 211 Ly s G31y I Ie 225 (31u Hi s O31n Le u Ser 305 Ph e ValI Ar g Tr p 1 95 O31n A a Ly s Pr o As p 275 vb t Le u Ser A a O31n 355 G31y Tr p Ar g G31y 245 Ly s Thr Ser Ly s Ar g 325 Ph e Ly s ValI 215 G31y Ph e Hi s cys ValI 295 A a A a ValI seql i st car r ect ed. t xt 1 O31n L eu Ala As p l'b t Hi s 200 205 Ser Ala Th r Pr o Val O31n 220 Phe Thr 03 y A a I I e 03 y 235 Thr O31y Ar g Leu Val O31y 250 Ala Val Ala Ala Leu Ala 265 Thr A a Ty r Ser As p Ser 280 285 O31y Thr Ala Val Ala Val 300 O31u Thr Ar g O31y Tr p As p 315 Thr Arg O31u Ty r O31y IIe 330 Ala O31y Tr p Ser Leu Ar g 345 A a A a ValI I Ie 255 li1e As n Pr o Ar g A a 335 Ar g A a O31n A a 240 Le u (31u As p As p As p 320 Le u Tr p <210O> 163 <21 1> 558 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (535) <223> RXN03105 <400> 163 ggt t ggggt c at caaaggat gcggacat cg ct gt ggggt t aggt gcct t t ct ggcaggt g aat caggact ct aagcaagc c ag O31n ggt O31y g ac As p CCC Pr o ggC O31y t cc Ser g ac As p gt c ValI t cc Ser tt a Le u gt c ValI cca Pr o tt t Ph e gca A a g aa O31u CCC Pr a ac g Thr gt a ValI ggt O31y cgt Ar g ccg tt a Pro Leu 15 gaa gat 31u As p cgg aaa Ar g Lys ttc agc Phe Ser Page 21~ gt gt aat aat t gcacct gt g t tg at t ct t cca gt t 115 Leu I Ie Leu Pro Val 1 cac ct g aat cac at c 163 Hi s Leu Asn Hi s I I e gaa gat ct c cgc ct c 211 O31u Asp Leu Arg Leu acc cac gaa ct c t tc 259 Thr Hi s O31u Leu Phe gtc cac tat gac acc 307 Val Hi s Tyr Asp Thr seql i tac act gca gac aaa tct cca att atc Tyr Thr Ala Asp Lys Ser Pro lie lie 75 gtg ctc acc gga gga tcc gga cac gcc Val Leu Thr y y Ser y His Ala ggc gaa ctc gca gca caa cga gcg gtc Sy u Leu Ala Ala n Arg Ala Val 105 110 agc gaa gac ttt cgg atc gcc tcg cat Ser G u Asp Phe Arg Ile Ala Ser His 120 125 acg tat aga aag cta acc ttt tta agt Thr Tyr Arg Lys Leu Thr Phe Leu Ser 135 140 at ct aacgct gag <210> 164 <211> 145 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 164 Leu Ile Leu Pro Val On Ou Gy Ile 1 5 His Leu Asn His lie G y G y Ser Arg G u Asp Leu Arg Leu Asp Arg Asp Ala 40 Thr His G u Leu Phe Pro G y Val Asn 55 Val His Tyr Asp Thr Tyr Thr Ala Asp 70 Val Asp Asn Val lie Val Leu Thr y Leu Ser Pro Ala Tyr G y G u Leu Al a 100 105 Thr Ser Pro Leu Tyr Ser G u Asp Phe 115 120 Ile Lys G u Arg Cys Thr Tyr Arg Lys 130 135 Phe 145 <210> 165 <211> 687 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS st cor r ect ed. t xt gac gcg gtt gac aat gtc att Asp Ala Val Asp Asn Val lie 80 ttc aag ctc tct cca gct tat Phe Lys Leu Ser Pro Ala Tyr 95 100 gga aac acc tcg ccg ctg tac Gy Asn Thr Ser Pro Leu Tyr 115 gaa cca atc aaa gag cgg tgc Su Pro lie Lys u Arg Cys 130 gcg cgg ttt tagggtgaga Al a Arg Phe 145 Ser 10 Leu Val Pr o Lys Gy 90 Al a Ar g Leu Tyr Ser Ser G u Ser Ser G n SIle Thr Phe Al a G u Pr o Pr o Gy Ar g Al a Phe 140 Pr o Hi s Phe Asn SIle Hi s Al a Ser 125 Leu Pr o G u Ar g Phe Asp Phe Gy G u Al a Page 213 seql i st cor r ect ed. t xt <222> (62)..(664) <223> RXS01130 <400> 165 agttcgtggc ggatgctgtg aacgtttccg gtggtcgcgt gggcgaagag gttctgtgtg g atg gat ctg gct cgc aag ctt ggt ctt ctt gct ggc aag ctt gtc gac 109 IVet Asp Leu Al a Arg Lys Leu G y Leu Leu Ala G y Lys Leu Val Asp 1 5 10 gcc gcc cca gtc tcc att gag gtt gag gct cga ggc gag ctt tct tcc 157 Ala Ala Pro Val Ser I le u Val G u Ala Arg Gy G u Leu Ser Ser 25 gag cag gtc aat gca ctt ggt ttg tcc gct gtt cgt ggt ttg ttc tcc 205 G u G n Val Asn Ala Leu y Leu Ser Ala Val Arg y Leu Phe Ser 40 gga att atc gaa gag tcc gtt act ttc gtc aac gct cct cgc att gct 253 Gy Ile Ile u u Ser Val Thr Phe Val Asn Ala Pro Arg lie Ala 55 gaa gag cgt ggc ctg gac atc tcc gtg aag acc aac tct gag tct gtt 301 G u G u Arg y Leu Asp Ile Ser Val Lys Thr Asn Ser u Ser Val 70 75 act cac cgt tcc gtc ctg cag gtc aag gtc att act ggc agc ggc gcg 349 Thr His Arg Ser Val Leu G n Val Lys Val lie Thr y Ser y Ala 90 agc gca act gtt gtt ggt gcc ctg act ggt ctt gag cgc gtt gag aag 397 Ser Ala Thr Val Val G y Ala Leu Thr y Leu G u Arg Val G u Lys 100 105 110 atc acc cgc atc aat ggc cgt ggc ctg gat ctg cgc gca gag ggt ctg 445 Ile Thr Arg Ile Asn G y Arg G y Leu Asp Leu Arg Al a G u G y Leu 115 120 125 aac ctc ttc ctg cag tac act gac gct cct ggt gca ctg ggt acc gtt 493 Asn Leu Phe Leu G n Tyr Thr Asp Ala Pro y Ala Leu y Thr Val 130 135 140 ggt acc aag ctg ggt gct gct ggc atc aac atc gag gct gct gcg ttg 541 Gy Thr Lys Leu Gy Ala Ala Gy Ile Asn Ile u Ala Ala Ala Leu 145 150 155 160 act cag gct gag aag ggt gac ggc gct gtc ctg atc ctg cgt gtt gag 589 Thr G n Ala G u Lys G y Asp y Ala Val Leu Ile Leu Arg Val G u 165 170 175 t cc gct gtc tct gaa gag ctg gaa gct gaa atc aac gct gag ttg ggt 637 Ser Ala Val Ser G u u Leu Gu Ala Gu lle Asn Ala Gu Leu y 180 185 190 gct act tcc ttc cag gtt gat ctt gac taattagaga tccatttgct 684 Ala Thr Ser Phe G n Val Asp Leu Asp 195 200 tga 687 <210> 166 <211> 201 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 166 IVet Asp Leu Ala Arg Lys Leu y Leu Leu Ala y Lys Leu Val Asp Page 214 seql i st cor r ect ed. t xt Ser Ile 3 u Val 10 Ala Arg G y G u Al a G u Gy G u Thr Ser SIle Asn Gy 145 Thr Ser Al a Pr o Val I le Ar g Ar g Thr Ar g 115 Phe Lys Al a Val Ser 195 Al a G u Leu Val Val Asn G n Gy Lys 165 G u G n Gy Val I le G n Al a Ar g Thr 135 Al a Asp Leu Asp G u Ser Phe Val Lys Thr 105 Leu Al a l Ie Al a Al a 185 Asp Al a Val Lys Val 90 Gy Asp Pr o Asn Val 170 G u Val Asn Thr 75 I le Leu Leu Gy SIle 155 Leu SIle Ar g Al a Asn Thr G u Ar g Al a 140 Su I e Asn Gy Pr o Ser Gy Ar g Al a 125 Leu Al a Leu Al a Leu Ser Ser Leu Phe Ser Ar g I e Al a G u Ser Val Ser G y Al a Val G u Lys 110 G u G y Leu G y Thr Val Al a Al a Leu 160 Arg Val G u 175 G u Leu G y 190 <210> 167 <211> 604 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(604) <223> RXS03112 <400> 167 gtgagcacgc aatttctttg ctgctgtcta ctgctcgcca gctgcgtgag ggcgagtgga agcggtcttc tttcaacggt aaa act gtc ggt atc gtc ggt ttt ggc Lys Thr Val Gy Ile Val y Phe y cag cgt ctt gct gcg ttt gag acc acc G n Arg Leu Ala Ala Phe G u Thr Thr 30 gct aac cct gct cgt gcg gct cag ctg Ala Asn Pro Ala Arg Al a Ala G n Leu 45 cac att His Il e 15 att gtt Ile Val aac gtt Asn Val Page 21 gatcctgctg ctgatgcgac gtg gaa att ttc gga Val Gu Ile Phe y 1 ggt cag ttg ttt gct G y G n Leu Phe Al a gct t ac gat cct t ac Ala Tyr Asp Pro Tyr gag ttg gtt gag ttg G u Leu Val G u Leu gat Asp acc Thr aag Lys gag Gu ggt Gy aag Lys gag Gu 150 gcg Al a ct g Leu gaa G u ggc Gy gct Al a gat Asp 120 cct Pr o caa G n gct Al a agc Ser gct Al a at c I e gct Al a t ac Tyr gtt Val cgt Ar g cgt Ar g ggc Gy 75 at c SIle gat Asp t cc Ser gtt Val gcg Al a 155 tct Ser at g NMt at c SIle gcg Al a acc Thr gt g Val 140 ggt Gy seql i st cor r ect ed. t xt gac ttt gtc acc att cac Asp Phe Val Thr Ile His ttt gat gcg cac ctc ctt Phe Asp Ala His Leu Leu 80 aac gct gct cgt ggt ggc Asn Ala Ala Arg Gy y 95 att gag tcc ggt cac att lie G u Ser G y Hi s Il e 110 gag cct tgc act gat tct G u Pro Cys Thr Asp Ser 125 130 act cct cac ttg ggt gct Thr Pro His Leu G y Al a 145 act gac att gct gat tct Thr Asp Ile Al a Asp Ser 160 ctt Leu gct Al a ctt Leu cgt Ar g 115 cct Pr o tct Ser gt g Val cct Pr o aag Lys gtt Val 100 ggc Gy ttg Leu act Thr ct c Leu aag Lys t cc Ser gat Asp gct Al a ttc Phe gaa Gu aag Lys 165 <210> 168 <211> 168 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 168 Val G u Ile Phe Gy Lys Thr Val C 1 5 Gy G n Leu Phe Ala G n Arg Leu A Ala Tyr Asp Pro Tyr Ala Asn Pro A 40 G u Leu Val G u Leu Asp G u Leu l 55 lie His Leu Pro Lys Thr Lys G u T 70 Leu Leu Ala Lys Ser Lys Lys y C Sy y Leu Val Asp u n Ala L 100 1 His lie Arg G y Ala G y Phe Asp 115 120 Asp Ser Pro Leu Phe Lys Leu Pro C 130 135 G y Al a Ser Thr G u G u Al a G n A 145 150 Sy IIe Val y Phe G y His IIe l a ~l a /et Fhr Sn eu lal Sn sp 10 Al a Phe G u Ar g Al a Al a Ser Arg Ser Al a a y let lie lie lle 90 Al a Asp Al a Tyr Ser Thr Val Val Val 140 Ar g Al a G y 155 Page 216 Thr G n Asp Phe Asn l Ie G u 125 Thr Thr I e Asn Val Al a Al a Ser Cys Hi s SIe seqI i st car r ect ed. t xt Asp Ser Val Leu Lys Al a Leu Al a 1 <210O> 169 <21 1> 1458 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1435) <223> RXN00969 <400> 169 ct at agt ggc taggt accct tt t tgt tt t ggacacat gt a t aggacaaca acgct cgacc gcgat tat tt t tggagaat c gggtggccga aacaaagtaa gcc Al a gcc Al a ac c Thr gt t ValI gca Al a g at As p gag G31u aat As n g aa G31u at t I Ie 1 50 c ag (31n gcc Al a ag c Ser tt a Le u t ac Tyr ggc G31y gag G31u g ac As p gt t ValI gct Al a 120 gca Al a gt g ValI gt g ValI g at As p aac As n tt c Ph e g at As p gct Al a ct c Le u gt c ValI gca Al a gt t ValI gt t ValI ggc G31y ggc G31y 1 70 ac c Thr ccc Pr o gga G31y gaa G31u gt t ValI act Thr 75 gt t ValI gct Al a gca Al a gac As p cca Pr o 155 at c (31y ggc G31y ac a Thr ct t Le u t ct Ser gag G31u gag G31u ct g Le u gct Al a ct g Le u 140 ct g Le u gt t ValI gct Al a ggt G31y ggc G31y c ac Hi s at c I Ie gct Al a at c I Ie gcc Al a 110 t ct Ser tt c Ph e cgc Ar g ggc G31y t at Tyr ggc G31y gag G31u at t I Ie aag Ly s gca Al a 80 ggc G31y aag Ly s gag G31u gct Al a ct g Le u 1 60 ac c Thr at g vb t 1 t ca Ser gt g ValI ggt G31y cca Pr o ct c Le u at t I Ie t ct Ser ct t Le u gct Al a 145 gct Al a aac As n acc t ca gca t ct Thr Ser Al a Ser gca gt c gga at t Al a Val G31y I Ie at g cgt ct g at g I'b t Ar g Leu I'b t ggc cca ct g gag G31y Pr o Leu G31u cgt gaa ggc gt t Ar g 31u G31y Val at c gag cgc gag Ilie G31u Ar g G31u gag t ac cca cgt G31u Tyr Pr o Ar g 1 00 gt t gt t acc gcc Val Val Thr Al a 115 gct gat gca gcg Al a Asp Al a Al a 130 gt t gca t gc gca Val Al a Cys Al a ggc gat cag at c 03 y Asp 03 n Il e 1 t tc at c t tg gac Phe Il e Leu Asp 1 gca gat t ct t tg gct gag gca Al aAsp Ser Leu Al a 0 u Al a Page 217 seql i st cor r ect ed. t xt act cgt ttg Thr Arg Leu 200 cat gac gcc Hi s Asp Al a 215 acc cgt gtt Thr Arg Val 230 aac gct gcc Asn Al a Al a ttg ttg gcc Leu Leu Al a att tct gct I e Ser Al a 280 gcg tcg gt a Al a Ser Val 295 gct ggt cgc Al a G y Ar g 310 gcg tct gct Al a Ser Al a cac ggt ggc Hi s G y G y gct gat ttc Al a Asp Phe 360 gaa gat cgc G u Asp Ar g 375 caa gga at c Gn G y lie 390 gca cgt ct g Al a Ar g Leu cgc acc gtt Arg Thr Val agt gtg at c Ser Val I le 440 <210> 170 t ac Tyr t cc Ser gcg Al a at t l Ie 250 tgt Cys gt g Val aag Lys at g Met ctt Leu 330 gct Al a gag G u ggg Gy ct g Leu gt g Val 410 ct g Leu gcc Al a aag Lys gat Asp 235 gag G u gag G u cac Hi s t cc Ser tt c Phe 315 ggc Gy cca Pr o acc Thr gtt Val cgt Ar g 395 gt c Val ct g Leu gaa G u gct Al a 220 gat Asp gca Al a aag Lys ccg Pr o ttt Phe 300 t ac Tyr gac Asp ggt Gy acc Thr ttg Leu 380 aca Thr acc Thr aag Lys gct Al a 205 gca Al a gt g Val gca Al a ttc Phe act Thr 285 aat Asn gga Gy gt c Val gag G u act Thr 365 gct Al a at c SIle cac Hi s gct Al a 190 gat Asp at t l Ie t ac Tyr cag G n acc Thr 270 ct a Leu gca Al a aac Asn gtt Val t cc Ser 350 cgt Ar g gaa G u cga Ar g tct Ser aag Lys 430 cca Pr o ttg Leu tgc Cys cag G n 255 aac Asn tta Leu at c l Ie ggt Gy ggt Gy 335 acc Thr t ac Tyr ttg Leu cag G n gcg Al a 415 cct Pr o act Thr gca Al a gaa G u 240 gca Al a aag Lys cct Pr o ttt Phe gca Al a 320 gcc Al a t ac Tyr cac Hi s gct Al a gaa G u 400 ct g Leu gtt Val gca Al a tgc Cys 225 ggt Sy ggc Sy gaa G u gt g Val gtt Val 305 ggt G y gca Al a gct Al a ct c Leu agc Ser 385 gag G u gaa G u gtt Val aac Asn 210 at c SIle at t SIle cac Hi s gga Sy t cc Ser 290 gaa G u ggc G y cga Ar g aac Asn gac Asp 370 ct g Leu cgc Ar g tct Ser aag Lys gt c Val gct Al a agg Ar g acc Thr aag Lys 275 cac Hi s gca Al a gcg Al a aac Asn ct g Leu 355 at g Met ttc Phe gat Asp gat Asp gca Al a 435 gaa G u ttc Phe aac Asn at c SIle 260 tcg Ser cca Pr o gaa G u cca Pr o aag Lys 340 ccg Pr o gat Asp tct Ser gat Asp ctt Leu 420 at c l Ie ggc G y cac Hi s at c SIe 245 aag Lys gct Al a ct g Leu gca Al a acc Thr 325 gt g Val at c SIe gt g Val gag G u gat Asp 405 t cc Ser aac Asn 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1458 cgc ctc gaa Arg Leu G u agg gac Arg Asp 445 taattttact gacatggcaa ttg Page 218 seql i st cor r ect ed. t xt <211> 445 <212> PRT <213> Corynebact eri um gl ut ai cum <400> 170 Met Thr Ser 1 Ser Val Gy Pr o Leu SIle Ser Leu Al a 145 Al a Asn Ser Al a Cys 225 Gy Gy G u Val Val 305 Gy Val Ar g Pr o G u G u Tyr Val 115 Asp Al a Asp SIle Al a 195 Val Al a Ar g Thr Lys 275 Hi s Al a Al a Ala Ser Ala Pro 5 G y I e Al a Leu Leu Met Thr G u Leu G u Val Arg Sy Val Al a Pro 70 Arg u Asp Val Pro Arg G u Val 100 Thr Al a Asn Lys Al a Al a G u Al a 135 Cys Ala lie Pro 150 Sn lie G n Ser 165 Leu Asp Al a Ibet 180 G u Ala Thr Arg G u G y Hi s Asp 215 Phe His Thr Arg 230 Asn lie Asn Al a 245 Ile Lys Leu Leu 260 Ser Ala i e Ser Pro Leu Ala Ser 295 G u Al a Al a G y 310 Pro Thr Ala Ser 325 Ser Leu Tyr 40 Sy G u Asp Val Al a 120 Al a Val Val Asp Leu 200 Al a Val Al a Al a Al a 280 Val Ar g Al a Phe Sy 25 Sy SIle Leu SIle Leu 105 Leu Asn Val Met Ser 185 Sy Al a Thr Asp l Ie 265 Ar g Asn Leu Val Asn 10 Phe Asp Al a Leu Val 90 Al a Val Val Sy Sy 170 Thr Tyr Ser Al a SIle 250 Cys Val Lys bet Pr o Sy G u Val Thr 75 Val Al a Al a Asp Pr o 155 I le Sy Al a Lys Asp 235 G u G u Hi s Ser Phe 315 Gy Thr Leu Ser G u G u Leu Al a Leu 140 Leu Val Al a G u Al a 220 Asp Al a Lys Pr o Phe 300 Tyr Lys Val Al a Asp Asp Val Lys Hi s 125 Tyr Ar g Asn Asp Al a 205 Al a Val Al a Phe Thr 285 Asn Sy Gy Sy Hi s SIle Al a SIe Al a 110 Ser Phe Ar g Sy Tyr 190 Asp l Ie Tyr G n Thr 270 Leu Al a Asn Pro G y Thr G u Arg Ile Ser Lys Phe Al a G y G y G y Lys Al a u G u Al a Ser Leu 160 Thr Thr 175 Al a Asp Pro Thr Leu Al a Cys u 240 G n Al a 255 Asn Lys Leu Pro lie Phe G y Al a 320 G y Al a 335 Leu G y Asp Val Val 330 Page 219 A a A a Le u Ser 385 G31u G31u ValI As n Le u 355 vb t Ph e As p As p A a 435 ValI I Ie ValI (31u As p 405 Ser As n Hi s A a O31u O31n 390 A a Ar g Ser G31y As p As p 375 G31y Ar g Th r ValI seql i st car r ect ed. t xt G31y Ar g Ala Pr o (31y (31u 345 Phe 03 y 03 u Th r Thr Thr 360 365 Ar g Val G31y Val Leu Ala 380 li1e Ser Leu Ar g Thr I Ie 395 Leu I Ie Val Val Thr Hi s 410 Val G31u Leu Leu Lys Ala 425 li1e Ar g Leu O31u Ar g As p 440 445 Th r Ty r Le u O31n A a 415 Pr o <210O> 171 <21 1> 493 <212> DNA <213> Cor ynebact er i urn gi ut ari cumn <220> <221 CDS <222> (493) <223> FRXA00974 <400> 171 ct at agt ggc taggt accct tt t tgt tt t ggacacat gt a t aggacaaca acgct cgacc gcgat tat tt t tggagaat c gggtggccga aacaaagtaa gcc A a gcc A a ac c Thr gt t ValI gca A a g at As p gag (31u ag c Ser tt a Le u t ac Tyr ggc G31y gag O31u g ac As p gt t ValI aac As n tt c Ph e g at As p gct A a ct c Le u gt c ValI gca A a ccc Pr o gga G31y gaa O31u gt t ValI act Thr 75 gt t ValI gct A a ggc G31y ac a Thr ct t Le u t ct Ser gag O31u gag O31u ct g Le u ggt G31y ggc G31y c ac Hi s at c I Ie gct A a at c I Ie gcc A a 110 ggc G31y gag O31u at t I Ie aag Ly s gca A a ggc G31y aag Ly s at g vb t 1 t ca Ser gt g ValI ggt G31y cca Pr o ct c Le u at t I Ie t ct Ser gca A a at g G31y cgt Ar g at c I Ie gag O31u gt t ValI acc t ca gca Thr Ser A a gt c ValI cgt Ar g 35 cca Pr o g aa O31u gag O31u t ac Tyr gt t ValI 115 gga G31y ct g Le u ct g Le u ggc G31y cgc Ar g cca Pr a 1 00 ac c Th r aat aag gct ct t gt t gca gct cac t ct gct gag ct t gct gat Page 220 seql i st cor r ect ed. t xt Asn Lys Ala Leu Val Al a Ala His Ser Ala G u Leu Ala Asp 120 125 130 <210> 172 <211> 131 <212> PRT <213> Cor ynebact er i um gl ut ari cum <400> 172 IVet Thr Ser Ala Ser Ala Pro Ser Phe Asn Pro 1 5 10 Ser Ala Val Gy I e Al a Leu Leu y Phe y 25 Val IVet Arg Leu IVet Thr G u Tyr G y Asp G u 40 Gy y Pro Leu u Val Arg Gy I e Ala Val Pro Arg G u y Val Al a Pro G u Leu Leu Thr 70 Leu Ile G u Arg G u Asp Val Asp Ile Val Val 90 Ile G u Tyr Pro Arg G u Val Val Leu Ala Al a 100 105 Ser Val Val Thr Ala Asn Lys Ala Leu Val Al a 115 120 Leu Al a Asp 130 <210> 173 <211> 1050 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1027) <223> RXA00970 <400> 173 gaatctgatc tttcccgcac cgttgaactg ctgaaggcta aacagt gt ga t ccgcct cga aagggact aa tttt act gac Lys Val Al a Asp Asp Val Lys Hi s 125 Gy Gy Hi s SIle Al a SIle Al a 110 Ser Pr o Thr Ar g Ser Phe Gy Gy Al a aac Asn ct c Leu act Thr ggc ggt Gy cct Pr o gaa Gu ggc aag Lys ttt Phe gaa gcu ggc gtt Val gac Asp at t SIle gaa acc Thr act Thr at t SIle gt c gt c Val tta Leu cca Pr o 45 cct gt a cct Val Pr o 15 ttg gca Leu Al a ggc ttg G y Leu gat ggc Page 221 agcctgttgt taaggcaat atg gca att gaa ctg IVet Ala I e G u Leu 1 gga tct tct gca aac G y Ser Ser Al a Asn ctg tcg gta tac gac Leu Ser Val Tyr Asp gaa gtg gaa gtt ttt G u Val G u Val Phe tcc cac ctg gtg gtt c 115 163 211 259 307 seql i st cor r ect ed. t xt Oy Ou Oy On Oy Ou Val Pro Leu Asp Gy Ser Hi 60 s Leu Val Val aaa gct Lys Al a ttg cga Leu Arg t cc tct Ser Ser gcg gat Al a Asp ttt gaa Phe G u 135 gtg gt g Val Val 150 tat gct Tyr Al a ctg gtt Leu Val ccc act Pro Thr gca gt g Al a Val 215 ggt act G y Thr 230 att acc Ile Thr tac ctt Tyr Leu att cca Ile Pro ctt gag Leu G u 295 at t l Ie gt g Val gct Al a ttc Phe 120 ggc G y tcg Ser gct Al a cct Pr o gaa G u 200 at g Met cgt Ar g tct Ser t cc Ser gac Asp 280 ctt Leu gct Al a tgc Cys gcg Al a ct g Leu cca Pr o aca Thr cca Pr o 170 ttc Phe act Thr gtt Val cgt Ar g tgg Tr p 250 gcc Al a gtt Val gtt Val ggc G y 75 cac Hi s gcg Al a act Thr gat Asp aat Asn 155 ctt Leu cac Hi s cac Hi s gcg Al a ct g Leu 235 gt a Val ggc G y tt g Leu gcg Al a ct g Leu aac Asn gtt Val caa G n aat Asn 140 ct g Leu gag G u gca Al a at c SIle ttg Leu 220 cac Hi s aac Asn cca Pr o gaa G u gga 30y 300 gca Al a at t SIle ggt G y 110 cag G n gcg Al a at c SIle cag G n acc Thr 190 gcg Al a cag G n cct Pr o ct g Leu gcc Al a 270 gct Al a gct Al a ccg Pr o 95 gtt Val at t SIle gct Al a gac Asp gac Asp 175 gaa G u cga Ar g cgt Ar g t at Tyr cgc Ar g 255 at g Met cgt Ar g gac Asp 80 cag G n gct Al a gtt Val tct Ser ggc G y 160 aat Asn gct Al a ttt Phe cct Pr o cgt Ar g 240 aac Asn gt g Val gag G u gct Al a t ct Ser gca Al a cag G n gt g Val 145 aag Lys at t SIle gt g Val aac Asn gat Asp 225 gca Al a cgt Ar g ct g Leu tct Ser gaa G u cgt Ar g gct Al a ttg Leu 130 ct g Leu agc Ser cgt Ar g cgc Ar g gt g Val 210 ttg Leu gaa G u ggc G y t cc Ser ggc G0y 290 gtt Val ggt G y aat Asn 115 t cc Ser ggt G y cag G n gcg Al a cga Ar g 195 t cc Ser ct g Leu gt g Val t ac Tyr act Thr 275 at t SIle cct Pr o ctt Leu 100 ggt G y t ct Ser gga G y cca Pr o act Thr 180 gt c Val cgc Ar g tgg Tr p ttg Leu gcg Al a 260 gag G u aag Lys gga G y ggc G y ttg Leu gcc Al a gca Al a cag G n 165 gcg Al a ctt Leu gtt Val gag G u cct Pr o 245 gca Al a cca Pr o gt g Val 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1050 cca gtc aag gtt gaa gtt aac caa cct Pro Val Lys Val G u Val Asn G n Pro taggcccaac aaggaaggcc ccc <210> 174 <211> 309 Page 222 seql i st cor r ect ed. t xt <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 174 bet Al a II e G u Leu Asn Val G y Arg Lys Val Thr Val Thr Val Pro 1 5 10 Sy Ser Ser Ala Asn Leu y Pro y Phe Asp Thr Leu y Leu Ala 25 Leu Ser Val Tyr Asp Thr Val G u Val G u Ile Ile Pro Ser y Leu 40 Gu Val Gu Val Phe Gy Gu Gy Gn Gy Gu Val Pro Leu Asp Gy 55 Ser His Leu Val Val Lys Ala lie Arg Ala y Leu Lys Ala Ala Asp 70 75 Ala G u Val Pro G y Leu Arg Val Val Cys His Asn Asn lie Pro G n 90 Ser Arg y Leu Gy Ser Ser Ala Ala Ala Ala Val Ala y Val Ala 100 105 110 Al a Al a Asn G y Leu Al a Asp Phe Pro Leu Thr G n G u G n I e Val 115 120 125 G n Leu Ser Ser Ala Phe G u G y His Pro Asp Asn Ala Ala Ala Ser 130 135 140 Val Leu y y Ala Val Val Ser Trp Thr Asn Leu Ser lie Asp y 145 150 155 160 Lys Ser G n Pro G n Tyr Ala Ala Val Pro Leu G u Val G n Asp Asn 165 170 175 Ile Arg Ala Thr Ala Leu Val Pro Asn Phe His Ala Ser Thr G u Ala 180 185 190 Val Arg Arg Val Leu Pro Thr G u Val Thr His lie Asp Ala Arg Phe 195 200 205 Asn Val Ser Arg Val Al a Val Met lie Val Ala Leu n G n Arg Pro 210 215 220 Asp Leu Leu Trp G u G y Thr Arg Asp Arg Leu His G n Pro Tyr Arg 225 230 235 240 Ala G u Val Leu Pro Ile Thr Ser G u Trp Val Asn Arg Leu Arg Asn 245 250 255 Arg y Tyr Ala Ala Tyr Leu Ser y Ala y Pro Thr Ala Met Val 260 265 270 Leu Ser Thr G u Pro IIe Pro Asp Lys Val Leu G u Asp Ala Arg G u 275 280 285 Ser y lie Lys Val Leu G u Leu G u Val Ala y Pro Val Lys Val 290 295 300 Su Val Asn n Pro 305 <210> 175 <211> 1566 <212> DNA Page 223 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1543) <223> RXA00330 <400> 175 gcaacacttt agggtatcgc gtgggcgaag tcaccttttt caacatattt gagacggtgt gggggagtat tgtgtcaccc cttgggatag ggttatatcc gtg gac tac att tcg Val Asp Tyr Ile Ser acg Thr ggc Gy caa G n gaa G u gac Asp t ac Tyr gac Asp aaa Lys cgc Ar g acc Thr 150 gt a Val cag G n ggc Sy gca Al a gat Asp ct a Leu gat Asp t ac Tyr cca Pr o aag Lys at t SIle at g Met 120 cgc Ar g t cc Ser at g Met ttt Phe ttc Phe 200 ttc Phe agc Ser cca Pr o gcc Al a gct Al a gaa G u aac Asn ct g Leu at g Met gaa G u gcg Al a acc Thr 170 ctt Leu gat Asp aaa Lys cgt Ar g gac Asp cag G n tt g Leu gac Asp 75 agc Ser ggc Gy cag G n acc Thr gaa G u 155 cca Pr o gac Asp tgc Cys gac Asp acc Thr ggc Sy ct g Leu gct Al a at c SIle gaa G u cac Hi s ct g Leu at c SIle 140 t ac Tyr gct Al a gat Asp caa G n aac Asn cct Pr o gga Sy agt Ser 45 gct Al a aag Lys gac Asp ctt Leu ct c Leu 125 aac Asn gcc Al a ggc Sy cca Pr o gac Asp 205 cgc Ar g gcc Al a ct g Leu aaa Lys gaa G u gcg Al a at c SIle t cc Ser 110 ggc G y at c SIle at g Met cgc Ar g aac Asn 190 gt a Val at c SIle cgc Ar g 15 t ac Tyr tgg Tr p gtt Val at c l Ie gtt Val 95 gaa G u gaa G u ct g Leu cgc Ar g at g Met 175 at c SIle gt c Val ttc Phe ct g Leu cgt Ar g at c SIle acc Thr cct Pr o ggc G y ctt Leu ggc Sy ggc Sy 160 acc Thr ttc Phe aag Lys 1 agt Ser cct Pr o gag G u t cc Ser gca Al a gt c Val cca Pr o ttc Phe gct Al a 145 cgc Ar g cca Pr o aac Asn gct Al a gat Asp gca Al a gt a Val ct g Leu cgc Ar g acc Thr acc Thr gaa G u 130 acc Thr gag G u ttc Phe at c SIle gt c Val 210 at t SIle acc Thr tta Leu ttt Phe gcc Al a gaa G u gct Al a 115 t ac Tyr tct Ser gga Sy cag G n gcc Al a 195 t cc Ser ttg Leu t ac Tyr gcc Al a gtt Val t ac Tyr ct c Leu 100 gca Al a gag G u ggc Sy at c SIe caa G n 180 ct c Leu gcc Al a ggt gcc gtg aac tcc atc aac Sy Ala Val Asn Ser Ile Asn Page 224 seql i st cor r ect ed. t xt tgg Tr p 230 cgc Ar g ggc Gy ctt Leu gac Asp acc Thr 310 ttc Phe aac Asn gat Asp gga Gy t cc Ser 390 gt g Val gaa G u ggt Gy cca Pr o t ac Tyr 470 cgc Ar g aca Thr ttc Phe at c SIle 280 ttc Phe gag G u cgt Ar g ct a Leu gcc Al a 360 t cc Ser ct c Leu cgc Ar g gca Al a gca Al a 440 aag Lys gt c Val at g Met agc Ser 250 gac Asp cgc Ar g cgt Ar g t cc Ser at c SIle 330 ggt Gy ttt Phe cat Hi s gt a Val tgg Tr p 410 cca Pr o caa G n t cc Ser gcg Al a gca Al a 235 aat Asn at t SIle ct c Leu acc Thr t ca Ser 315 tt c Phe acc Thr gag G u gct Al a ct a Leu 395 agg Ar g gt g Val act Thr gac Asp at t l Ie 475 220 cag G n gac Asp tgc Cys at c SIle ggc Sy 300 cct Pr o gac Asp cag G n aag Lys gac Asp 380 at c SIle gac Asp aaa Lys cca Pr o ct a Leu 460 gca Al a gtt Val caa G n gca Al a gt g Val 285 gac Asp tcg Ser ct g Leu gtt Val gct Al a 365 cgt Ar g gat Asp gag G u ttt Phe gag G u 445 cca Pr o aac Asn gt g Val aag Lys ggc Sy 270 gcc Al a t ac Tyr at g let ct c Leu cgc Ar g 350 gca Al a gt g Val ccc Pr o gt c Val gcc Al a 430 cgt Ar g aac Asn act Thr t ac Tyr gt c Val 255 cac Hi s acc Thr cga Ar g gat Asp ggc G y 335 caa G n gca Al a gca Al a cac Hi s aac Asn 415 gac Asp ttc Phe gac Asp t cc Ser t ac Tyr 240 agc Ser at c SIle aac Asn gt c Val at c SIle 320 cgc Ar g ggc G y gaa G u acc Thr acc Thr 400 acc Thr acc Thr gcc Al a acc Thr gt g Val 480 225 gtt Val ttc Phe gcc Al a gaa G u cgc Ar g 305 t cc Ser gac Asp gga G y t ac Tyr at c SIle 385 gcc Al a cca Pr o at c SIle gcg Al a gat Asp 465 aag Lys t cc Ser t cc Ser cgc Ar g aac Asn 290 agc Ser cgc Ar g gcc Al a ttc Phe ggt G y 370 gct Al a gac Asp at c SIle gt c Val at c l Ie 450 gca Al a t ca Ser gt a Val caa G n 275 gat Asp t cc Ser gcc Al a acc Thr t ca Ser 355 ttc Phe gac Asp ggc G y at c SIle gaa G u 435 at g Met gtt Val tgg Tr p cca Pr o 260 at g Met gt g Val gca Al a t cc Ser cgc Ar g 340 ct g Leu gcc Al a gt g Val gtt Val gt c Val 420 gca Al a gat Asp aag Lys 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1553 1566 t aact t gct t tacgccaagg cct Page 225 seql i st cor r ect ed. t xt <210> 176 <211> 481 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 176 Val Asp Tyr lie Ser Thr Arg Asp Ala 1 Ser Pr o G u Ser Al a Val Pr o Phe Al a 145 Ar g Pr o Asn Al a Val 225 Val Phe Al a G u Ar g 305 SIle Thr Leu Phe Al a G u Al a 115 Tyr Ser Gy G n Al a 195 Ser Ser Ser Val G n 275 Asp Ser 5 Leu Pr o Asn Asp Thr G u Phe Leu Asp Ar g 165 Al a Asp Asp Asn SIle 245 Thr Gy Leu Asp Gy Leu Gy I le Pr o Asn Asp Ar g 135 Gy Phe bet Val G u 215 Al a Thr Asn Pr o G u 295 Hi s Al a Asp Al a Val Phe Tyr 105 Al a Asn Ser Leu Gy 185 Asp Lys Leu Thr Gy 265 Asp Phe Thr Ser 10 Pr o Al a Al a G u Asn 90 Leu Met G u Al a Thr 170 Leu Asp Lys Met Ser 250 Asp Ar g Ar g Ser Ar g Asp G n Leu Asp Ser Gy G n Thr G u 155 Pr o Asp Cys Asp Al a 235 Asn SIle Leu Thr Ser 315 Pr o Gy Ser Al a Lys Asp Leu Leu 125 Asn Al a Gy Pr o Asp 205 Ar g Val G n Al a Val 285 Asp Ser Al a Leu Lys G u Al a SIle Ser 110 Gy SIle Met Ar g Asn 190 Val SIle Val Lys Gy 270 Al a Tyr Met Ar g Tyr Tr p Val SIe Val G u G u Leu Ar g bet 175 I e Val Gy Tyr Val 255 Hi s Thr Ar g Asp Ser Arg Ala Ser Asn Phe u Arg Phe Ile Phe Asp Leu Leu Gy Arg Page 226 Asp Gy Tyr SIle 385 Al a Pr o SIle Al a Asp 465 Lys Thr Ser 355 Phe Asp Gy SIle G u 435 let Val 325 Val Al a Ser Hi s Hi s 405 Leu SIle Al a G n Asp Asp Ar g 375 Ar g Al a Thr G u Phe 455 I le seql i st cor r ect ed. t xt 330 Leu Phe G y Thr G n Val 345 Al a Asn Phe G u Lys Al a 360 365 Ser Thr His Ala Asp Arg 380 Leu Asp Val Leu Ile Asp 395 Arg G n Trp Arg Asp G u 410 Ala Leu Pro Val Lys Phe 425 Ala Pro G n Thr Pro G u 440 445 Lys Val Ser Asp Leu Pro 460 Val Asp Ala lie Ala Asn 475 Ar g 350 Al a Val Pr o Val Al a 430 Ar g Asn Thr 335 G n Al a Al a Hi s Asn 415 Asp Phe Asp Ser <210> 177 <211> 1254 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1231) <223> RXN00403 <400> 177 tttttcagac tcgtgagaat gcaaactaga cta aagttttagt cttgtccacc cagaacaggc ggt cct Pr o gcc Al a gaa G u cac Hi s ct c Leu ggt Gy gca Al a cgc Ar g ct c Leu ccc Pr o ctt Leu at t l Ie gat Asp gga Gy aaa Lys gaa G u aca Thr aaa Lys gat Asp gcc Al a at c SIle aac Asn gaa G u t cc Ser at c l Ie caa G n gct Al a gga Gy 45 aac Asn aac Asn gcg Al a gaa G u cgc Ar g gca Al a act Thr igacagag ctgtccatat acactggacg tattttc atg ccc acc ctc gcg IVet Pro Thr Leu Ala 1 atc ggt gat gtc tcc acc gaa I e y Asp Val Ser Thr u 15 atc gcc tat cac cgc tgg ggt I I e Ala Tyr His Arg Trp G y agc aat gtc gtt ctc atc gaa Ser Asn Val Val Leu Ile G u gcc gat tgg tgg gct gac ttg Ala Asp Trp Trp Ala Asp Leu gat att tac tgc gtg atc tgt Asp lie Tyr Cys Val lie Cys Page 227 seql i st cor r ect ed. t xt acc aac gtc atc Thr Asn Val lile ggt Gy ggt tgc aac ggt Sy Cys Asn y acc gga cct ggc Thr y Pro y t cc atg Ser bet 100 cat cca gat Hi s Pro Asp cgt gat cag Arg Asp n 120 aat ttc tgg ggt Asn Phe Trp y aat Asn 110 cgc ttc ccc gcc Arg Phe Pro Ala acg tcc att Thr Ser I e 115 ct c ggc at c Leu G y Ile gta aac gcc gaa Val Asn Ala u aaa Lys 125 caa ttc ctc gac G n Phe Leu Asp gca Al a 130 acc acg Thr Thr 135 gtc gcc gca gta Val Ala Ala Val ctt Leu 140 ggt ggt tcc atg G y G y Ser 'bet ggt gcc cgc acc G y Ala Arg Thr ct a Leu 150 gag tgg gcc gca G u Trp Ala Ala tac cca gaa act Tyr Pro G u Thr gtt Val 160 ggc gca gct gct G y Al a Al a Al a ctt gca gtt tct Leu Al a Val Ser gca Al a 170 cgc gcc agc gcc Arg Ala Ser Ala caa atc ggc att Sn lie G y lle caa t cc G n Ser 180 gcc caa att Ala G n Ile tac tac gaa Tyr Tyr G u 200 gcg att gaa aac Ala lle G u Asn gac Asp 190 cac cac tgg cac His His Trp His gaa ggc aac G u G y Asn 195 gcc gcc cga Ala Ala Arg tcc ggc tgc aac Ser y Cys Asn cca Pr o 205 gcc acc gga ctc Al a Thr G y Leu ggc G1y 210 cgc at c Arg Ile 215 gcc cac ctc acc Ala His Leu Thr tac Tyr 220 cgt ggc gaa cta Arg Gy G u Leu atc gac gaa cgc Ile Asp u Arg ttc Phe 230 ggc acc aaa gcc Sy Thr Lys Ala aag aac gaa aac Lys Asn G u Asn cca Pr o 240 ctc ggt ccc tac Leu y Pro Tyr aag ccc gac cag Lys Pro Asp G n cgc Ar g 250 ttc gcc gtg gaa Phe Ala Val G u tac ttg gac tac Tyr Leu Asp Tyr caa gca G n Al a 260 gac aag cta Asp Lys Leu gac gcc ctc Asp Al a Leu 280 cag cgt ttc gac G n Arg Phe Asp gcc Al a 270 ggc tcc tac gt c G y Ser Tyr Val ttg ctc acc Leu Leu Thr 275 ggc ctc aac G y Leu Asn aac cgc cac gac Asn Arg His Asp ggt cgc gac cgc Sy Arg Asp Arg gga Gy 290 aag gca Lys Ala 295 ctc gaa tcc at c Leu u Ser Ile aaa Lys 300 gtt cca gtc ctt Val Pro Val Leu gca ggc gta gat Ala y Val Asp acc Thr 310 gat att ttg tac Asp Ile Leu Tyr ccc tac cac cag caa gaa cac ctc tcc aga aac Pro Tyr His n G n G u His Leu Ser Arg Asn 315 320 325 1027 1075 1123 1171 ctg gga aat cta Leu G y Asn Leu ct g Leu 330 gca atg gca aaa Al a IVet Ala Lys gta tcc cct gtc Val Ser Pro Val ggc cac G y Hi s 340 gat gct ttc ctc acc gaa agc cgc caa Asp Ala Phe Leu Thr u Ser Arg G n atg gat cgc atc gtg agg aac IVet Asp Arg lie Val Arg Asn Page 228 seqI i st car r ect ed. t xt 345 350 355 t tc t tc agc ct c at c t cc cca gac gaa gac aac cct t cg acc t ac at c Phe Phe Ser Leu Ilie Ser Pro Asp O31u Asp Asn Pro Ser Thr Tyr I Ie 360 365 370 gag t tc t ac at c t aat aggt at t tacgacaaa t ag O31u Phe Tyr Ilie 375 <210> 178 <211> 377 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1219 1254 <400> 178 I'bt Pro Thr Leu Ala Pro Ser G31y O31n As p Tyr ValI Tr p Ty r G31y Pr o As p G31y 145 Gy le Tr p Le u O31u 225 Le u Le u Ser Ar g Le u A a ValI G31y Thr 115 Le u A a A a I Ie O31u 1 95 A a As p Pr o Tyr O31u G31y O31u Le u cys vb t I Ie I Ie Thr ValI 1 65 Ser As n Ar g Ar g Ar g 245 A a A a O31u Hi s Le u 70 Thr Hi s Ar g Thr Le u 150 Le u A a Tyr Ar g Ph e 230 Ly s As p G31y Tyr A a G31y As n Pr o As p Thr 135 O31u A a O31n Tyr I Ie 215 O31y Pr a Ly s A a Ar g 40 Le u Pr a ValI As p O31n 120 ValI Tr p ValI I Ie (31u 200 A a Thr As p Le u lIe ValI Thr G31y I Ie G31y 1 05 ValI A a A a Ser Ly s 1 85 Ser Hi s Ly s O31n ValI 265 Le u 10 I Ie As p O31y Ly s O31y 90 As n As n A a A a A a 1 70 A a O31y Le u A a Ar g 250 O31u Thr Ly s As p A a O31y Ph e A a ValI vb t 1 Ar g I Ie cys Th r O31n 235 Ph e I Ie As n O31u Ser li1e cys Tr p O31u Le u 140 Tyr A a O31u As n Tyr 220 Ly s A a O31n A a O31y As n As n As n O31y Ly s 125 O31y Pr a Ser As n Pr a 205 Ar g As n ValI A a O31u Ar g A a Thr O31y As n 110 O31n O31y O31u A a As p 1 A a O31y O31u O31u lIe lie Ser A a As p Ser Ar g Ph e Ser Thr Tr p 1 Hi s Th r O31u As n Se r 255 O31n Ar g Ph e Asp Ala O31y Se r 270 Page 229 seqi i st car r ec Ty r Val Leu Leu Thr Asp Ala Leu Asn Arg His 275 280 Ar g 31y G31y Leu Asn Lys Ala Leu O31u Ser I Ie 290 295 Val Ala G31y Val As p Thr As p I Ie Leu Ty r Pr o 305 310 315 Hi s Leu Ser Ar g As n Leu G31y As n Leu Leu Ala 325 330 Ser Pr o Val G31y Hi s As p Ala Phe Leu Thr (31u 340 345 Ar g I Ie Val Ar g As n Phe Phe Ser Leu I Ie Ser 355 360 Pr o Ser Thr Ty r I Ie (31u Phe Ty r I Ie 370 375 <210O> 179 <21 1> 1210 <212> DNA <213> Cor ynebact er i urn gi ut ari cumn <220> <221 ODS <222> (1210) <223> FRXA00403 <400> 179 t tt tt cagac t cgt gagaat gcaaact aga ct agacagag aagtt t tagt ct tgt ccacc cagaacaggc ggt tatt t tc ted. t xt Asp I I e 285 Lys Val 300 Ty r Hi s [vb t A a Ser Ar g Pr o As p 365 Ar g ValI O31n I Ie 335 vb t As p ct gtccat at acact ggacg ccc acc ct c Pr o Thr Leu cct Pr o gcc A a g aa G31u c ac Hi s ct c Le u ac c Thr cat Hi s ggt G31y gca A a cgc Ar g ct c Le u ccc Pr o gt c ValI g at As p ct t Le u at t I Ie g at As p gga G31y aaa Lys5 ggt G31y aat As n gaa G31u aca Thr aaa Lys5 g at As p gcc A a 75 ggt G31y t t c Ph e at c I Ie aac As n g aa G31u t cc Ser at c I Ie t gc cys t gg Tr p c aa O31n gct A a gga G31y 45 aac As n aac As n aac As n ggt G31y gcg A a g aa O31u cgc Ar g gca A a act Thr ggt G31y aat As n 110 at c lIe 15 at c I Ie ag c Ser gcc A a g at As p t cc Ser 95 cgc Ar g ggt G31y gcc A a aat As n g at As p at t I Ie 80 ac c Thr tt c Ph e gt c ValI c ac Hi s gt t ValI t gg Tr p t gc cys cct Pr o gcc A a t cc Ser cgc Ar g ct c Le u gct A a gt g ValI ggc G31y ac g Thr 115 ac c Thr t gg Tr p at c I Ie g ac As p at c I Ie t cc Ser 1 00 t cc Ser gcg A a g aa O31u ggt G31y g aa O31u tt g Le u t gt cys at g vb t at t I Ie cgt gat cag gt a aac gcc gaa aaa caa t tc ct c gac gca ct c ggc at c Page 230 Arg Asp Sn Val Asn Al a u acc Thr ct a Leu 150 ctt Leu gcc Al a t ac Tyr cgc Ar g ttc Phe 230 aag Lys gac Asp gac Asp aag Lys acc Thr 310 ct g Leu gat Asp ttc Phe 120 gt c Val tgg Tr p gtt Val at t l Ie gaa G u 200 gcc Al a acc Thr gac Asp ct a Leu ct c Leu 280 ct c Leu at t SIle aat Asn ttc Phe agc Ser 360 gca Al a gca Al a gca Al a 170 gcg Al a ggc G y ct c Leu gcc Al a cgc Ar g 250 cag G n cgc Ar g t cc Ser t ac Tyr ct g Leu 330 acc Thr at c SIle gt a Val at g bet 155 cgc Ar g at t l Ie tgc Cys acc Thr caa G n 235 tt c Phe cgt Ar g cac Hi s at c SIle ccc Pr o 315 gca Al a gaa G u t cc Ser ctt Leu 140 t ac Tyr gcc Al a gaa G u aac Asn t ac Tyr 220 aag Lys gcc Al a ttc Phe gac Asp aaa Lys 300 t ac Tyr at g Met agc Ser cca Pr o S( Lys 125 ggt G y cca Pr o agc Ser aac Asn cca Pr o 205 cgt Ar g aac Asn gt g Val gac Asp at t SIle 285 gtt Val cac Hi s gca Al a cgc Ar g gac Asp 365 ggt G y gaa G u gcc Al a gac Asp 190 gcc Al a ggc Gy gaa G u gaa G u gcc Al a 270 ggt G y cca Pr o cag G n aaa Lys caa G n 350 gaa G u eql i st cor r ect ed. t xt G n Phe Leu Asp Al 13 a Leu G y I e 0 t cc Ser act Thr tgg Tr p 175 cac Hi s acc Thr gaa G u aac Asn t cc Ser 255 ggc G y cgc Ar g gt c Val caa G n at c SIle 335 at g Met gac Asp at g let gtt Val 160 caa G n cac Hi s gga G y ct a Leu cca Pr o 240 t ac Tyr t cc Ser gac Asp ctt Leu gaa G u 320 gt a Val gat Asp aac Asn ggt G y gca Al a ggc G y cac Hi s ggc G y 210 at c SIle ggt G y gac Asp gt c Val gga G y 290 gca Al a ct c Leu cct Pr o at c SIle tcg Ser 370 gcc Al a gct Al a at t SIle gaa G u 195 gcc Al a gac Asp ccc Pr o t ac Tyr ttg Leu 275 ggc G y ggc G y t cc Ser gt c Val gt g Val 355 cgc Ar g gct Al a caa G n 180 ggc G y gcc Al a gaa G u t ac Tyr caa G n 260 ct c Leu ct c Leu gt a Val aga Ar g ggc G y 340 agg Ar g acc Thr gtt Val 165 t cc Ser aac Asn cga Ar g cgc Ar g cgc Ar g 245 gca Al a acc Thr aac Asn gat Asp aac Asn 325 cac Hi s aac Asn 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1210 <210> 180 <211> 370 <212> PRT <213> Corynebacter i um gl utam cum Page 231 seqi i st car r ect ed. t xt <400> 180 vb t As p Tyr ValI Tr p Tyr G31y Pr o As p G31y 145 Gy le Tr p Le u G31u 225 Le u Le u Tyr Ar g ValI 305 Hi s Ser Pr o Thr Leu Val Ser Thr Hi s Ar g Tr p Val Leu I Ie Tr p Ala As p Cys Val I Ie Pro G31y Ser 1 00 Al a Thr Ser 115 Al a Leu G3 y 130 G31y Ala Ar g A a A a A a G31y I Ie G31n 1 80 Hi s G3 u G3 y 1 95 G3 y A a A a 210 1 1 e As p a3 u a31y Pr o Ty r As p Ty r a3 n 260 Val Leu Leu 275 a3 y a3 y Leu 290 A a a3 y Val Leu Ser Ar g Pr o Val a31v A a (31u a31y a31u Le u Cys vb t le le Thr ValI 1 65 Ser As n Ar g Ar g Ar g 245 A a Thr As n As p As n 325 Hi s Pr o Ser a31y a31n A a a31u Hi s Le u 70 Thr Hi s Ar g Thr Le u 150 Le u A a Tyr Ar g Ph e 230 Lys5 As p As p Lys5 Thr 310 Le u As p (31y Tyr A a 55 a31y As n Pr o As p Thr 135 a31u A a a31n Tyr I Ie 215 a31y Pr o Lys5 A a A a 295 As p a31y A a A a Ar g 40 Le u Pr a ValI As p a31n 120 ValI Tr p ValI I Ie a31u 200 A a Thr As p Le u Le u 280 Le u I Ie As n Ph e lIe 25 ValI Thr a31y I Ie a31y 1 05 ValI A a A a Ser Lys5 1 85 Ser Hi s Lys5 a31n ValI 265 As n a31u Le u Le u Le u 345 Le u 10 lIe As p a31y Ly s a31y 90 As n As n A a A a A a 1 70 A a a31y Le u A a Ar g 250 a31n Ar g Ser Tyr Le u 330 a3 u I I e a3 n A a Thr Ly s As p A a a31y Ph e A a ValI vb t 1 Ar g I Ie Cys Thr a31n 235 Ph e Ar g Hi s I Ie Pr a 315 A a As n a31u Ser li1e Cys Tr p a31u Le u 140 Tyr A a a31u As n Tyr 220 Ly s A a Ph e As p Ly s 300 Tyr vb t A a a31y As n As n As n a31y Ly s 125 a31y Pr a Ser As n Pr a 205 Ar g As n ValI As p I Ie 285 ValI Hi s A a (31u Ar g A a Thr a31y As n 110 a31n a31y a31u A a As p 1 A a a31y a31u a31u A a 270 a31y Pr a a31n Ly s lIe lie Ser A a As p Ser Ar g Ph e Ser Thr Tr p 1 Hi s Thr a31u As n Ser 255 a31y Ar g ValI a31n I Ie 335 Th r a31u Se r Ar g a31n lbt As p 350 Page 232 seqi i st car r ect ed. t xt Ar g I Ie Val Ar g Asn Phe Phe Ser Leu I Ie Ser Pro Asp (31u Asp Asn 355 360 365 Pr o Ser 370 <210> 181 <211> 771 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221> CDS <222> (748) <223> RXCO1207 <400> 181 ct tcat gat c t caccggcag agcgcgt t tt gt tacagcgc gt aaact gt g act t tgaaaa at tt t tgaac aat ccgt aca ccaact tcag gagaaaaaca gt g agc aga at Val Ser Arg II c t at e Tyr g ac As p g at As p t at Tyr ct g Le u ggC G31y gca A a gt t ValI ac c Th r cgc Ar g act Th r 1 50 gt c ValI gcc A a gt c ValI ct c Le u ac c Th r t gg Tr p gcg A a c ag O31n at g vb t 120 ac c Th r cca Pr o gt c ValI c aa G n gcc A a t gc cys c ac Hi s ac c Th r gt g ValI cca Pr o cgc Ar g cca Pr o ac c Th r ct c Le u 1 70 t cc Ser c ag O31n gct A a ggc G31y c aa O31n gcg A a ct t Le u cca Pr o gct A a 140 ct g Le u ggt G31y gca A a gt t ValI aac As n g at As p ct t Le u t gg Tr p t gg Tr p 110 c ac Hi s t cc Ser gct A a g aa O31u gca A a 15 gt c ValI aac As n at g vb t gt g ValI cct Pr a 95 aac As n cca Pr a t cc Ser cgt Ar g t gc cys 1 75 ggc G y ct t Le u gag O3u ccc Pr a c ac Hi s ggt O3y ct t Le u gt t ValI gcc A a c ag O3n 1 gcg A a 1 ct a Le u ccc Pr a gca A a gt t ValI t cc Ser gga G31y G31y gcc A a aac As n 145 c ag O31n ct g Le u aag Ly s ac g Th r gt a ValI cca Pr a t at Ty r ct g Le u g at As p at t I Ie 130 at c I Ie ct c Le u gcc A a gcg A a g at As p gcc A a gt g ValI t ct Ser t cc Ser ac c Th r 115 g aa O31u t cc Ser aac As n ac c Th r gct A a ac c Th r aac As n ct c Le u gcg A a at c I Ie 1 00 cgt Ar g tt g Le u gga G31y c aa O31n cct Pr a 1 Page 233 seqI i st car r ect ed. t xt acc atc gt g gat at t t ca ggc ccc gca cca aag at t t tg cgt gag ggt Thr I Ie Val Asp I Ie Se r G31y Pr o Ala Pro Lys Ilie Leu Ar g O31u 31y 185 190 195 gcc atc agc gca gaa cgc gtt ggc gaa gta ctt gga gtg tcg gca gaa Ala Ile Ser Aia 1u Ar gVal G31y 31u Val Leu (1y Val Ser Aia (1u 200 205 210 agc ct g cgc t aaat gggag t cggt t tcgc ggg Ser Leu Arg 215 <210> 182 <211> 216 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 182 ValI Le u Pr o A a ValI Ser G31y G31y A a As n 145 O31n Le u I Ie O31y Ser Ar g I Ie Lys A a A a Thr As p Thr Val Ala As n Pr o Val Leu Ty r Ser A a Leu Ser I Ie 1 00 As p Thr Ar g 115 1 1 e 03 u Leu 130 1 1 e Ser 03 y Leu As n 03 n Ala Thr Pr o 1 80 Leu Ar g (31u 1 95 Val Ser A a Tyr ValI Le u Le u ValI O31n li1e G31y Le u Hi s As n 1 65 Ser G31y O31u Asp Cys As p A a Ty r G y Leu A a 55 0 y Ser 70 A a Lys Val Pr o Thr Val Ar g O n 135 Thr Pr o 150 Val Ala Thr I Ie A a I I e Ser Leu 215 A a ValI Le u 40 Thr Tr p A a O31n vb t 120 Thr Pr o ValI ValI Ser 200 Ar g As p Ly s 25 G y Ly s As p Le u A a 1 05 Le u 0Gy Thr Tyr As p 1 85 A a O31n 10 A a Cys Hi s Thr ValI 90 Pr o Ar g Pr a Thr Le u 1 70 li1e (31u As p G31y As p Ar g I Ie O31u Ser vb t vb t ValI 1 As p Ser Ar g Ser O31n A a O31y O31n A a Le u Pr a A a 140 Le u O31y O31y ValI Ar g Le u Ph e Pr a O31y Ph e Pr a Le u 125 ValI O31u O31y Pr a O31y 205 A a ValI As n As p Le u Tr p Tr p 110 Hi s Ser A a O31u A a 1 O31u A a ValI As n vb t ValI Pr a As n Pr a Ser Ar g Cys 1 Pr a ValI <210O> 183 <211> 1419 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> Page 234 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(1396) <223> RXC00152 <400> 183 gtcattgata tccaaggcac gaccgcgatt gtatggaaag caaatagtac tggccattcc caactaaaac tggagtaacg ct c Leu at a SIle agc Ser gt g Val ttc Phe gat Asp ggc G y ctt Leu cgt Ar g acc Thr 150 ccg Pr o cgt Ar g gac Asp gag G u at a SIle ct g Leu acc Thr cga Ar g ccg Pr o gt g Val gac Asp gac Asp 120 gt g Val tgg Tr p cca Pr o aag Lys aaa Lys 200 gaa G u ttc Phe ccc Pr o acc Thr cgc Ar g gct Al a acc Thr t ac Tyr gt c Val aac Asn ct g Leu at c SIle 170 gcc Al a gcc Al a cac Hi s ct g Leu cag G n gtt Val gca Al a gtt Val 75 tt c Phe at c SIle ggt G y cgc Ar g cgc Ar g 155 cag G n acc Thr gaa G u gca Al a gt c Val ggt G y t ca Ser agg Ar g at t SIle caa G n gt c Val ggc G y cgc Ar g 140 at c SIle caa G n at t SIle ggt G y t cc Ser 220 ttt Phe gaa G u ggt G y 45 at c SIle acc Thr at c SIle ggt G y at g Met 125 ct c Leu agc Ser tcg Ser ttg Leu gaa G u 205 at c SIle gt c Val gcc Al a ggc G y gac Asp caa G n aac Asn gt g Val 110 acc Thr cgt Ar g cgt Ar g at g Met acc Thr 190 aag Lys ct g Leu gcc Al a 15 gcc Al a ct g Leu acc Thr gac Asp gaa G u 95 gag G u ct g Leu ggc G y gt g Val gaa 175 gca Al a caa G n aac Asn gt c Val gt c Val acc Thr cgt Ar g aac Asn 80 cca Pr o cag G n gaa G u gag G u gaa G u 160 aag Lys gaa G u gcc Al a gca Al a aagcctaaat ttttaacaat atg aca gga cta atc 11! IVet Thr G y Leu I e 1 gtg gtg atc aag tcc 16 Val Val i e Lys Ser att gaa cgc ctt ggt 21 lie u Arg Leu y ctg ctg gtt cca ttc Leu Leu Val Pro Phe gag cgc gtg gtc tca G u Arg Val Val Ser ctg acc gtg gcc atc Leu Thr Val Ala lle gag cgc gcc atc tac Su Arg Ala lie Tyr 100 att tct gta gca aca Ile Ser Val Ala Thr 115 gaa acc ctc act tca 49! G u Thr Leu Thr Ser 130 ctc gat gca gca acc 54 Leu Asp Al a Al a Thr 145 cta aag gca att gat 59! Leu Lys Ala lie Asp 165 cag atg aag gca gac 64; G n IVet Lys Ala Asp 180 ggt cag cgc gaa gcc 69 G y n Arg G u Ala 195 aag atc ctc caa gct 73! Lys Ile Leu G n Ala 210 gaa gca gaa cgc caa 78 Su Ala u Arg n 225 gcg atg atc ctg cgc gcc gaa ggt gaa cgc gca gca cgc tac ctc cag Page 235 seql i st cor r ect ed. t xt Al a Iet Ile Leu Arg Al a G u y G u Arg Al a Al a Arg Tyr Leu G n 230 235 240 245 gcg cag ggt gaa gcc cga gca atc caa aag gtc aac gca gca atc aag 883 Ala n G y G u Ala Arg Ala lie On Lys Val Asn Ala Ala lle Lys 250 255 260 tct gcc aag ttg acc cca gag gtt ctt gct tat caa tac ctc gaa aag 931 Ser Ala Lys Leu Thr Pro G u Val Leu Ala Tyr G n Tyr Leu G u Lys 265 270 275 ctt cct aag atc gca gag ggc aac gcc tcc aag at g tgg gtc atc cca 979 Leu Pro Lys lie Ala u y Asn Ala Ser Lys IVet Trp Val Ile Pro 280 285 290 agc cag ttc tcc gat tct ctg gaa ggt ttt gcg aag cag ttc ggc gca 1027 Ser G n Phe Ser Asp Ser Leu G u y Phe Ala Lys G n Phe y Ala 295 300 305 aag gat gca gaa ggt gtc ttc cgc tac gaa cca aac acc gtg gat gaa 1075 Lys Asp Ala G u y Val Phe Arg Tyr G u Pro Asn Thr Val Asp G u 310 315 320 325 gaa acc cgc gac atc gca aac gcc gac aac gtg gaa gac tgg ttc tcc 1123 G u Thr Arg Asp Ile A a Asn Ala Asp Asn Val G u Asp Trp Phe Ser 330 335 340 acc gaa tca gac cct gaa atc gca gca gca gtc gcc gca gca aac gcc 1171 Thr u Ser Asp Pro G u Ile Ala Ala Ala Val Ala Ala Ala Asn Ala 345 350 355 gtg gcc aac aag cca gtc gat cca gaa ccc ggt gag atc ctt tcc aag 1219 Val Ala Asn Lys Pro Val Asp Pro u Pro y u lie Leu Ser Lys 360 365 370 aag acc gca cga cgc gtt gaa cct gaa gca gta ttg gag gct ttg caa 1267 Lys Thr Ala Arg Arg Val G u Pro G u Ala Val Leu G u Ala Leu G n 375 380 385 aac gga acc act aca caa cct gag gtt gag gca gca cct cct acc gca 1315 Asn y Thr Thr Thr G n Pro G u Val G u Ala Ala Pro Pro Thr Ala 390 395 400 405 aac ttc gcc caa gaa ttc cct gca cca cag gca aac cct gaa gat tac 1363 Asn Phe Ala n G u Phe Pro Ala Pro G n Ala Asn Pro G u Asp Tyr 410 415 420 tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416 Ser Asp G n His Arg G u Asn Pro Tyr G y Asn 425 430 cgg 1419 <210> 184 <211> 432 <212> PRT <213> Cor ynebact eri um glut arri cum <400> 184 IVet Thr G y Leu I e Leu Al a I le Val Phe Leu Val Phe Val Ala Val 1 5 10 Val Val Ile Lys Ser I e Ala Leu Ile Pro n y u Ala Ala Val 25 lie G u Arg Leu G y Ser Tyr Thr Arg Thr Val Ser G y G y Leu Thr 40 Page 236 seqi i st car r ect ed. t xt Leu Leu Val Pro Phe Val Asp Arg Val Ar g Al a Ar I Ie Asp Th r Ar g O31u Le u O31u I Ie O31u Le u 145 Le u (31n O31y Ly s O31u 225 Al a As n O31n vb t Ly s 305 As n O31u Al a O31u Le u 385 ValI ValI Al a ValI 115 Le u Al a Al a Ly s Ar g 1 95 Le u O31u Tyr Al a Le u 275 ValI Ph e ValI Tr p Al a 355 Le u Al a Ser lIe Tyr Thr Ser Thr As p 1 65 As p Al a Al a O31n O31n 245 Ly s Ly s Pr o Al a O31u 325 Ser Al a Ly s O31n Ph e 70 As p O31y Le u Ar g Thr 150 Pr o Ar g As p O31u Al a 230 Al a Ser Le u Ser Ly s 310 O31u Thr ValI Ly s As n 390 Pr o I Ie ValI Ar g As p 135 Ly s Pr o G u I Ie O3y 215 vb t O3n Al a Pr a O3n 295 As p Thr O3u Al a Thr 375 O3y Pr a ValI As p As p 120 ValI Tr p Pr a Ly s Ly s 200 Ou le Oy Ly s Ly s 280 Ph e Al a Ar g Ser As n 360 Al a Thr O31n ValI As n 1 ValI I Ie O31y Ser Ar g 1 Thr Ly s Le u O31u Le u 265 li1e Ser O31u As p As p 345 Ly s Ar g Thr Al a Thr 90 Tyr ValI As n Le u I Ie 1 70 Al a Al a Hi s Ar g Al a 250 Thr Al a As p Oy le 330 Pr a Pr a Ar g Thr ValI Ph e I Ie O31y Ar g Ar g 1 O31n Thr O31u Al a Al a 235 Ar g Pr a O31u Ser ValI 315 Al a O31u ValI ValI O31n 395 Thr I Ie O31y vb t 125 Le u Ser Ser Le u O31u 205 li1e Oy le ValI As n 285 O31u Ar g Al a Al a Pr a 365 Pr a O31u As p O31u O31u Le u O31y ValI O31u 1 Al a O31n As n Ar g Ly s 255 Al a Ser Ph e O31u As n 335 Al a Pr a Al a O31u Ala Pro Pro Thr Ala Asn Phe Ala On Ou Phe Pro Ala Pro On Ala Page 237 seql i st cor r ect ed. t xt 405 410 415 Asn Pro G u Asp Tyr Ser Asp G n His Arg G u Asn Pro Tyr y Asn 420 425 430 <210> 185 <211> 1170 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1147) <223> RXA00115 <400> 185 tggattctcg agt ct gt aca cccttgatca aagcccgagt cgtatattgt gacctacacc ccatactgtt aggagttttc gt t ccgtaga t t aact t t gt at g ct c gac IVet Leu Asp ttt Phe cgg Ar g t ac Tyr at t SIle at c SIle at c SIle gat Asp gat Asp gag G u gag G u 150 gcg Al a acc Thr gaa G u aca Thr acg Thr act Thr caa G n agc Ser gtt Val 120 ttt Phe at t SIle act Thr gag G u gaa G u gga Sy t ac Tyr cat Hi s ggt Gy tcg Ser cag G n gt c Val cgc Ar g aag Lys gtt Val gaa G u acg Thr t cc Ser gcg Al a 75 aat Asn at g Met gac Asp gtt Val tt c Phe 155 aac Asn cag G n ggg G y ct c Leu gga G y ct g Leu ggt Sy t cc Ser cgg Ar g ggt Sy 140 cct Pr o act Thr cca t ac Pro Tyr 15 gtg att Val lile gag gac Su Asp cat cag Hi s G n cca t ca Pro Ser tcg gtc Ser Val 95 ttc ccg Phe Pro 110 ttg cgc Leu Arg tcg atg Ser lbet caa gtt G n Val cat gat His Asp gaa G u gag G u aag Lys acc Thr aag Lys 80 t cc Ser aat Asn caa G n ggt Sy cat Hi s 160 1 acc Thr gat Asp t cc Ser tgg Tr p t at Tyr cct Pr o gtt Val gag G u gcg Al a 145 cga Ar g gct Al a tgc Cys aac Asn ttc Phe ttc Phe gcc Al a cgc Ar g ttt Phe 130 cag G n gca Al a t cc Ser tgg Tr p gcc Al a cag G n at c SIle aac Asn at t SIle 115 ggt Sy caa G n gct Al a aat agt Asn Ser att ggc I e y ttg gct Leu Al a at c ct c II e Leu cag tac G n Tyr atc tcc Ile Ser acg gct Thr Al a 100 ggt gat Sy Asp att acc Ile Thr acc t at Thr Tyr ccg atc Pro Ile 165 cag act G n Thr ttc atc ttc acc Phe Ile Phe Thr Page 238 seql i st cor r ect ed. t xt ctt Leu t cc Ser tgg Tr p cgt Ar g 230 gat Asp tgg Tr p gca Al a agc Ser ct c Leu 310 ctt Leu aat Asn gag G u cat Hi s 200 gcc Al a ct g Leu ct g Leu tgg Tr p gct Al a 280 gac Asp cca Pr o ctt Leu aaa Lys gtt Val gag G u gga Sy ctt Leu at g Met 250 cat Hi s ggc Sy ttc Phe agc Ser aac Asn 330 gag G u gt a Val ttt Phe gaa G u 235 t cc Ser ggc Sy cga Ar g ttt Phe gag G u 315 gt c Val gcc Al a gct Al a t cc Ser 220 agt Ser at g Met gat Asp gt g Val cct Pr o 300 ctt Leu tct Ser cca Pr o 190 gga Sy gag G u gag G u cct Pr o tct Ser 270 gct Al a cgt Ar g gt g Val aat 175 ggg Gy ctt Leu ttc Phe t ca Ser aat Asn 255 cgc Ar g aag Lys gac Asp at c SIle t ac ttc aat ggc Phe Asn G y cgc cgt caa Arg Arg G n 210 tgg aag cag Trp Lys G n 225 gtg ctc gcg Val Leu Al a 240 acc ttg ctc Thr Leu Leu cac acc ggc Hi s Thr G y acc ttc gtt Thr Phe Val 290 tgt gcc gca Cys Al a Al a 305 gaa gac at c G u Asp Ile 320 at c cca cag Ile Pro n ggc gaa t ac G y G u Tyr 195 tcg cat ct t Ser Hi s Leu gag gca tgg G u Al a Trp gac ttc ctg Asp Phe Leu 245 aac aac gct Asn Asn Al a 260 ggc gac ttg G y Asp Leu 275 atg ccc at c IVet Pro lie gaa caa gca G u G n Al a gcc ggt cac Al a G y Hi s 325 atc gac aaa I e Asp Lys 340 691 739 787 835 883 931 979 1027 1075 1123 1170 Asn Tyr 335 ctg ttc gag agc Leu Phe G u Ser t aaacact ga t gt caaagag cct <210> 186 <211> 349 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 186 IVet Leu Asp Asn Ser Phe Tyr Thr Al a 1 5 Thr Ala Ser Ile y Arg Leu G u Leu 25 Asp Cys Trp Leu Ala Tyr Ala Thr Ala 40 Ser Asn Ala lie Leu Ile Pro Thr Trp 55 Trp Phe n n Tyr Ile y Thr Asp 70 Su Val On y Pro Tyr u 10 Gu Gu Gy y Val Ile u Sy Thr Leu Asn G u Asp Lys Tyr Ser G y Thr His G n Thr His Ala Leu Asp Pro Ser Lys 75 Page 239 seqI i st car r ect ed. t xt Tyr Phe Ilie lieSer lieAsn On Ilie GyAsn Gy Leu 90 Ser Val Ser Pr o ValI O31u A a 145 Ar g I Ie As n Ar g Ly s 225 Le u Le u Thr Ph e A a 305 As p Pr o As n I Ie 115 G31y O31n A a Thr G31y 1 95 Ser O31u As p As n G31y 275 vb t O31u A a I Ie A a As p Thr Tyr I Ie 1 65 Thr Tyr Le u Tr p Le u 245 A a Le u I Ie A a Hi s 325 Ly s As p As p O31u O31u 150 A a Le u Ser Tr p Ar g 230 As p Tr p A a Ser Le u 310 Le u As n As p ValI Le u 135 Tr p G31y As n Ser A a 215 Ar g Pr o Ly s A a O31u 295 li1e G31y Le u Ser ValI 120 Ph e I Ie Thr O31u Hi s 200 A a Le u Le u Tr p A a 280 As p Pr o Le u Ly s I Ie 1 05 A a A a ValI A a Thr 1 85 O31u vb t G31y Ph e O31n 265 Le u vb t G31y Ph e (31u 345 vb t As p ValI Ph e 1 As n (31u ValI Ph e (31u 235 Ser G31y Ar g Ph e (31u 315 ValI Ph e Ser Ar g G31y 140 Pr a Thr A a A a Ser 220 Ser vb t As p ValI Pr a 300 Le u Ser (31u Ly s Le u 125 G31y As p Pr a As p As p 205 Thr Ly s As p ValI Ly s 285 ValI Ar g (31u Ser Ph e 110 Le u Ser O31n Hi s Pr a 1 O31y O31u O31u Pr a Ser 270 A a Ar g ValI As n Pr a Ar g vb t ValI As p 1 O31y Le u Ph e Ser As n 255 Ar g Ly s As p I Ie Tyr 335 <210O> 187 <21 1> 1254 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1231) <223> RXN00403 <400> 187 t tt t tcagac t cgt gagaat gcaaact aga ct agacagag ct gt ccat at acact ggacg aagtt t tagt ct tgt ccacc cagaacaggc ggt tatt t tc at g ccc acc ct c gcg 115 N/t Pr o Thr Leu Ala 1 Page 240 seql i st cor r ect ed. t xt cct t ca ggt caa Pro Ser Gy G n gcc Al a gaa G u cac Hi s ct c Leu acc Thr cat Hi s cgt Ar g acc Thr ct a Leu 150 ctt Leu gcc Al a t ac Tyr cgc Ar g ttc Phe 230 aag Lys gac Asp gca Al a cgc Ar g ct c Leu ccc Pr o gt c Val gat Asp cag G n 120 gt c Val tgg Tr p gtt Val at t l Ie gaa G u 200 gcc Al a acc Thr gac Asp ct a Leu ctt gaa atc caa gcg atc ggt Leu G u lie G n Ala I e G y 15 att aca aac gct gaa atc gcc lle Thr Asn Ala G u Ile Al a gat aaa gaa gga cgc agc aat Asp Lys u G y Arg Ser Asn 45 gga gat tcc aac gca gcc gat G y Asp Ser Asn Ala Ala Asp aaa gcc atc aac act gat att Lys Ala lle Asn Thr Asp Ile 75 ggt ggt tgc aac ggt tcc acc G y G y Cys Asn G y Ser Thr 95 aat ttc tgg ggt aat cgc ttc Asn Phe Trp y Asn Arg Phe 110 aac gcc gaa aaa caa ttc ctc Asn Al a G u Lys G n Phe Leu 125 gca gta ctt ggt ggt tcc atg Al a Val Leu G y G y Ser Vbet 140 gca atg tac cca gaa act gtt Ala IVet Tyr Pro G u Thr Val 155 160 gca cgc gcc agc gcc tgg caa Ala Arg Ala Ser Ala Trp G n 170 175 gcg att gaa aac gac cac cac Ala lle G u Asn Asp His Hi s 190 ggc tgc aac cca gcc acc gga Gy Cys Asn Pro Ala Thr G y 205 ctc acc tac cgt ggc gaa cta Leu Thr Tyr Arg Gy G u Leu 220 gcc caa aag aac gaa aac cca Ala n Lys Asn u Asn Pro 235 240 cgc ttc gcc gtg gaa tcc tac Arg Phe Ala Val u Ser Tyr 250 255 cag cgt ttc gac gcc ggc tcc G n Arg Phe Asp Ala Gy Ser 270 gat gtc t cc Asp Val Ser tat cac cgc Tyr His Arg gtc gtt ctc Val Val Leu tgg tgg gct Trp Trp Al a tac tgc gtg Tyr Cys Val gga cct ggc G y Pro G y ccc gcc acg Pro Al a Thr 115 gac gca ct c Asp Al a Leu 130 ggt ggt gcc G y G y Al a 145 ggc gca gct G y Al a Al a atc ggc att lie G y lle t gg cac gaa Trp Hi s G u 195 ct c ggc gcc Leu G y Al a 210 gaa atc gac G u Ile Asp 225 ct c ggt ccc Leu G y Pr o ttg gac tac Leu Asp Tyr tac gtc ttg Tyr Val Leu 275 acc Thr tgg Tr p at c SIe gac Asp at c SIe t cc Ser 100 t cc Ser ggc Gy cgc Ar g gct Al a caa G n 180 ggc G y gcc Al a gaa G u t ac Tyr caa G n 260 ct c Leu Page 241 seql i gac gcc ctc aac cgc cac gac att ggt Asp Al a Leu Asn Arg His Asp IIe G y 280 285 aag gca ctc gaa tcc atc aaa gtt cca Lys Ala Leu u Ser Ile Lys Val Pro 295 300 acc gat att ttg tac ccc tac cac cag Thr Asp Ile Leu Tyr Pro Tyr His G n 310 315 ctg gga aat cta ctg gca atg gca aaa Leu G y Asn Leu Leu Al a IVet Ala Lys 330 gat gct ttc ctc acc gaa agc cgc caa Asp Ala Phe Leu Thr u Ser Arg G n 345 350 ttc ttc agc ctc atc tcc cca gac gaa Phe Phe Ser Leu Ile Ser Pro Asp G u 360 365 gag ttc tac atc taataggtat ttacgaca; Su Phe Tyr Ile 375 <210> 188 <211> 377 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 188 Met Pro Thr Leu Ala Pro Ser Gy G n 1 5 Asp Val Ser Thr u Ala Gy Ala l Ie 25 Tyr His Arg Trp G y G u Tyr Arg Val 40 Val Val Leu Ile G u His Ala Leu Thr Trp Trp Ala Asp Leu Leu y Pro y 70 Tyr Cys Val lie Cys Thr Asn Val lie G y Pro G y Ser IVet His Pro Asp G y 100 105 Pro Ala Thr Ser lie Arg Asp G n Val 115 120 Asp Ala Leu Gy Ile Thr Thr Val Al a 130 135 Gy y Ala Arg Thr Leu G u Trp Al a 145 150 G y Ala Ala Ala Val Leu Ala Val Ser 165 st cor r ect ed. t xt cgc gac cgc gga Arg Asp Arg G y 290 gtc ctt gtc gca Val Leu Val Al a 305 caa gaa cac ct c Sn G u His Leu 320 atc gta tcc cct Ile Val Ser Pro 335 atg gat cgc atc IVet Asp Arg Ile gac aac cct tcg Asp Asn Pro Ser 370 aa tag ggc Gy ggc G y t cc Ser gt c Val gt g Val 355 acc Thr Al a G u Ar g Al a Thr Gy Asn 110 G n Gy G u ct c Leu gt a Val aga Ar g ggc G y 340 agg Ar g t ac Tyr 979 1027 1075 1123 1171 1219 1254 Leu 10 SIle Asp Sy Lys Sy 90 Asn Asn Al a Al a G u Thr Lys Asp Al a 75 Sy Phe Al a Val bet 155 G n Al a Sy Asn Asn Asn Sy Lys 125 G y Pr o I e y I e Al a Ser Asn Al a Asp Asp I e Ser Thr Arg Phe Phe Leu Ser bet Thr Val 160 Trp n 175 Ala Arg Ala Ser Ala 170 Page 242 seqI i st car r ect ed. t xt II e Gy HIe Gn Ser Al a G n Il e 1 80 Tr p Le u O31u 225 Le u Le u Ty r Ar g ValI 305 Hi s Ser Ar g Pr o O31u 1 95 A a As p Pr o Ty r Le u 275 G31y G y Ser ValI ValI 355 Th r As n Ar g Ar g Ar g 245 A a Th r As n As p As n 325 Hi s As n I Ie Ty r I Ie 215 G31y Pr o Ly s A a A a 295 As p G31y A a Ph e Ph e 375 Ly s 1 85 Ser Hi s Ly s O31n ValI 265 As n (31u Le u Le u Le u 345 Le u I Ie cys Th r O31n 235 Ph e Ar g Hi s I Ie Pr a 315 A a O31u Ser As n Ty r 220 Ly s A a Ph e As p Ly s 300 Ty r vb t Ser Pr o Pr a 205 Ar g As n ValI As p I Ie 285 ValI Hi s A a Ar g As p 365 A a I I e 0 u Asn As p Hi s Hi s 1 A a G31y O31u O31u A a 270 G31y Pr a G n Ly s O31n 350 O31u Th r O31u As n Ser 255 G31y Ar g ValI G n I Ie 335 vb t As p <210O> 189 <21 1> 1210 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1210) <223> FRXA00403 <400> 189 t tt tt cagac t cgt gagaat gcaaact aga ct agacagag ct gt ccat at acact ggacg aagtt t tagt ct tgt ccacc cagaacaggc ggt tatt t tc at g ccc acc ctc gcg I'b t Pr o Thr Leu Ala 1 cct t ca ggt caa ct t gaa atc caa gcg at c ggt gat gt c t cc acc gaa Pr o Ser G31y O31n Leu O31u I Ie O31n Ala Ilie G31y Asp Val Ser Thr O31u 15 gcc gga gca atc at t aca aac gct gaa at c gcc t at cac cgc t gg ggt Ala (1y Ala IIe Ilie Th r As n Aia (1u Ilie Ala Tyr Hi s Ar g Tr p G31y 30 gaa t ac cgc gta gat aaa gaa gga cgc agc aat gt c gt t ct c at c gaa 31u Ty rAr gVa Asp Lys (1u (1y Ar gSer Asn Va VaLeu Ile (1u Page 243 seql i st cor r ect ed. t xt cac gcc Hi s Al a ctc act gga gat Leu Thr G y Asp t cc Ser aac gca gcc gat Asn Al a Al a Asp tgg gct gac ttg Trp Ala Asp Leu ct c Leu ggt ccc ggc aaa G y Pro G y Lys gcc Al a 75 atc aac act gat I e Asn Thr Asp at t Ile tac tgc gtg atc Tyr Cys Val lle acc aac gtc at c Thr Asn Val lle ggt Gy ggt tgc aac ggt Sy Cys Asn y acc gga cct ggc Thr Gy Pro y t cc atg Ser bet 100 tcc att Ser Ile cat cca gat Hi s Pro Asp cgt gat cag Arg Asp n 120 aat ttc tgg ggt Asn Phe Trp G y aat Asn 110 cgc ttc ccc gcc Arg Phe Pro Ala gta aac gcc gaa Val Asn Ala u aaa Lys 125 caa ttc ctc gac G n Phe Leu Asp gca Al a 130 ct c ggc at c Leu G y Ile acc acg Thr Thr 135 gtc gcc gca gta Val Ala Ala Val ctt Leu 140 ggt ggt tcc atg G y G y Ser 'bet ggt gcc cgc acc G y Ala Arg Thr ct a Leu 150 gag tgg gcc gca G u Trp Ala Ala tac cca gaa act Tyr Pro G u Thr gtt Val 160 ggc gca gct gct G y Al a Al a Al a ctt gca gtt tct Leu Al a Val Ser gca Al a 170 cgc gcc agc gcc Arg Ala Ser Ala caa atc ggc att Sn lie G y lle caa t cc G n Ser 180 gcc caa att Ala G n Ile tac tac gaa Tyr Tyr G u 200 gcg att gaa aac Ala lie G u Asn gac Asp 190 cac cac tgg cac His His Trp His gaa ggc aac G u G y Asn 195 gcc gcc cga Ala Ala Arg tcc ggc tgc aac Ser y Cys Asn cca Pr o 205 gcc acc gga ctc Al a Thr G y Leu ggc G1y 210 cgc at c Arg Ile 215 gcc cac ctc acc Ala His Leu Thr tac Tyr 220 cgt ggc gaa cta Arg Gy G u Leu atc gac gaa cgc Ile Asp u Arg ttc Phe 230 ggc acc aaa gcc Sy Thr Lys Al a aag aac gaa aac Lys Asn G u Asn cca Pr o 240 ctc ggt ccc tac Leu y Pro Tyr aag ccc gac cag Lys Pro Asp G n cgc Ar g 250 ttc gcc gtg gaa Phe Ala Val G u tac ttg gac tac Tyr Leu Asp Tyr caa gca G n Al a 260 gac aag cta Asp Lys Leu gac gcc ctc Asp Al a Leu 280 cag cgt ttc gac G n Arg Phe Asp gcc Al a 270 ggc tcc tac gt c G y Ser Tyr Val ttg ctc acc Leu Leu Thr 275 aac cgc cac gac Asn Arg His Asp att ggt cgc gac cgc gga ggc ctc aac lie G y Arg Asp Arg G y G y Leu Asn 285 290 aag gca Lys Al a 295 ctc gaa tcc at c Leu u Ser Ile aaa Lys 300 gtt cca gtc ctt Val Pro Val Leu gca ggc gta gat Ala y Val Asp 1027 1075 acc gat att ttg tac ccc tac cac cag Thr Asp Ile Leu Tyr Pro Tyr His G n caa gaa cac ctc tcc aga aac Sn G u His Leu Ser Arg Asn Page 244 310 ct g gga aat Leu 03 y Asn gat gct tt c Asp Al a Phe t tc t tc ag c Phe Phe Ser 360 ct g Le u 330 ac c Thr at c I Ie 315 gca Al a gaa O31u t cc Ser seqI i st car r ect ed. t xt 320 gca aaa at c gt a t cc cct Al a Lys I Ie Val Ser Pr o 335 cgc caa atg gat cgc atc Arg O31n I'b t Asp Ar g Ilie 350 gac gaa gac aac cct t cg Asp O31u Asp Asn Pr o Ser 365 370 325 gt c ggc cac Val G31y Hi s 340 gt g agg aac Val Ar g Asn 355 1123 1171 1210 <210O> 190 <21 1> 370 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 190 I'bt Pr o Thr Leu Al a Pr o Ser G31y O31n As p Tyr ValI Tr p Tyr G31y Pr o As p G31y 145 Gy le Tr p Le u O31u 225 Le u Ser Ar g Le u Al a ValI G31y Thr 115 Le u Al a Al a I Ie O31u 1 95 Al a As p Pr o O31u G31y O31u Le u cys vb t I Ie I Ie Thr ValI 1 65 Ser As n Ar g Ar g Ar g 245 Al a O31u Hi s Le u 70 Thr Hi s Ar g Thr Le u 150 Le u Al a Tyr Ar g Ph e 230 Ly s G31y Tyr Al a G31y As n Pr o As p Thr 135 O31u Al a O31n Tyr I Ie 215 G31y Pr o Al a Ar g 40 Le u Pr a ValI As p O31n 120 ValI Tr p ValI I Ie O31u 200 Al a Thr As p lIe ValI Thr O31y I Ie O31y 1 05 ValI Al a Al a Ser Lys5 1 85 Ser Hi s Lys5 (31n Le u 10 I Ie As p O31y Lys5 O31y 90 As n As n Al a Al a Al a 1 70 Al a O31y Le u Al a Ar g 250 O31u Thr Lys5 As p Al a 75 O31y Ph e Al a ValI vb t 1 55 Ar g I Ie cys Thr O31n 235 O31n Al a O31y As n As n As n O31y Lys5 125 O31y Pr a Ser As n Pr a 205 Ar g As n Al a O31u Ar g Al a Thr O31y As n 110 O31n O31y O31u Al a As p 1 Al a O31y O31u lIe lie Ser Al a As p Ser Ar g Ph e Ser Thr Tr p 1 Hi s Thr O31u As n O31y Al a As n As p I Ie Thr Ph e Le u vb t ValI 1 O31n Hi s O31y Le u Pr a 240 Phe Al a Val (31u Ser Tyr 255 Page 245 seqI i st car r ec Leu Asp Tyr O31n Al a Asp Lys Leu Val O31n Ar g 260 265 Tyr Val Leu Leu Thr Asp Al a Leu As n Ar g Hi s 275 280 Ar g 31y G31y Leu Asn Lys Al a Leu O31u Se r Ilie 290 295 Val Al a G31y Val Asp Thr Asp Ilie Leu Tyr Pr o 305 310 315 His Leu Ser Arg Asn Leu G31y Asn Leu Leu Ala 325 330 Ser Pr o Val G31y Hi s Asp Al a Phe Leu Thr O31u 340 345 Ar g Ilie Val Ar g Asn Phe Phe Ser Leu Ilie Ser 355 360 Pr o Ser 370 <210> 191 <211> 687 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221> OIDS <222> (664) <223> RXS03158 <400> 191 caaagctcac cgaaggcacc aacgccaagt tggttgttga ted. t xt Phe As p As p I I e 285 Lys Val 300 Ty r Hi s [vb t Al a Ser Ar g Pr o As p 365 Al a 270 G31y Pr o O31n Lys5 O31n 350 (31u G31y Ar g ValI O31n I Ie 335 vb t As p caacacct tg gcat ccccat acct gcagca gccact aaaa ct cggcgcac acgcaagt cc t tg cac t cc acc acc Leu Hi s Ser Thr Thr aag aac As n gga O31y aag at c I Ie cca Pr o at c I Ie c ag O31n at c lIe ct t Le u g aa O31u ct g Le u gga O31y at g t ca Ser gt g ValI ct g Le u aac As n t cc Ser g aa O31u tt c Ph e at g vb t t cc Ser cca Pr o g ac As p g aa O31u g at As p 45 g at As p cgc Ar g ggC O31y gt t ValI ct g Le u gca Al a cgc Ar g cca Pr a c ac Hi s gt t ValI 15 ct g Le u t ac Tyr c ac Hi s gag O31u g aa O31u 95 ggC O31y tt c Ph e ct g Le u t gc cys gt c ValI gt c ValI 1 ggC O31y at g vb t ac c Thr g ac As p t cc Ser gca Al a ct t Le u C ag O31n gcc Al a aac As n ac c Thr gcg Al a ggt O31y ggC O31y O31y g aa O31u ct c Le u C ag O31n 1 00 aag cgc ttc ggc ggc atg atc tcc gtc cgt ttc gca ggc ggc gaa gaa Page 246 Ly s gca Al a t cc Ser c ac Hi s 1 50 cgc Ar g gag (31u Ph e aag Ly s 120 ggt G31y t ca Ser t cc Ser gcc Al a G31y tt c Ph e gt g ValI gcc Al a ggt G31y 1 70 aat As n I Ie ac c Thr t cc Ser 140 t ct Ser g aa O31u ct t Le u seqI i st car r ect ed. t xt Ser Val Ar g Ph e Al a 31y (31y 110 115 t cc acc aaa ctg at c t gt ct g Ser Thr Lys Leu I Ie Cys Leu 125 130 ctc CCtg gag cac cca gca acc Leu Leu 31u Hi s Pr o Al a Thr 145 cag ctc gag gtt ccc cgc gac O31n Leu O31u Val Pr o Ar g Asp 1 gac at t gaa gac ct g ct c gca Asp Il e 03 u Asp Leu Leu Al a 1 75 t agaaact at ttggcggcaa gca O31u gcc Al a at g vb t ct c Le u g at As p 1 <210O> 192 <211> 188 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 192 Leu Hi s Ser O3 y Leu Val [Vb t O31n (1y Thr Al a Ar g As p As n Al a Ser Thr Val Al a Al a Lys Al a 03 y 03 y 115 1 1 e Cys Leu 130 Pr o Al a Thr 145 Pr o Ar g As p Leu Leu Al a <210> 193 Thr Thr Lys Tyr lie Ou Gy 10 G31y Th r As n Asp O31n 31u [Vb t 25 G31y I Ie G31y Pr o I Ie Pro Ser 40 G31y Leu Lys Thr Leu Ala Val O3 u Lys I I e Al a 03 u Phe Leu 70 Leu Ty r Pr o G31y Leu Lys Asn 90 O31n I'b t Lys Ar g Phe G31y G31y 100 105 O3 u 03 u Al a Al a Lys Lys Phe 120 Ala O31u Ser Leu G31y G31y Val1 135 Nib t Thr Hi s 03 n Ser Al a Al a 150 Leu Val Ar g I Ie Ser I Ie G31y 165 170 As p Val O31u O31n Al a Leu As n 180 185 Hi s As p ValI Ar g As p Hi s vb t Cys O31u O31y 1 li1e As n As p O31u As p As p Ar g O31y Ser Ser 125 Le u O31n As p ValI Le u Al a Ar g Pr o Hi s ValI 110 Thr Le u Le u I Ie ValI Le u Tyr Hi s O31u O31u Ar g O31u O31u O31u 1 Page 247 seqI i st car r ect ed. t xt <21 1> 617 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (594) <223> FRXA00254 <400> 193 cag cca ct a O31n Pro Leu t ac at c gga Tyr Ilie (31y gac cag gaa Asp O31n O31u ccg at c cca Pro Ilie Pr o acc ct t gca Thr Leu Al a gcg gaa ttc Al a 03 u Phe ggt ct g aag 03 y Leu Lys cgc t tc ggc Ar g Phe (31y 115 gct aag aag Al a Lys Lys 130 ct c ggt ggc Leu 03 y 03 y 145 cag t ca gct 03 n Ser Al a at c t cc at t Ilie Ser Ilie cag gcc ct C 03 n Al a Leu 1 ct c Le u c ac Hi s g ac As p gt t ValI cgc Ar g g ac As p c ac Hi s at g vb t t gt cys g aa O31u G31y 1 65 at t I Ie ggC G31y t cc Ser gaa O31u t t c Ph e at g vb t 70 t cc Ser cca Pr o at c I Ie acc Thr t cc Ser 150 t ct Ser gaa (31u gca Al a g ac As p g aa O31u g at As p 55 g at As p cgc Ar g ggC G31y t cc Ser t cc Ser 135 ct c Le u c ag O31n g ac As p c ac Hi s gt t ValI ct g Le u 40 gca Al a cgc Ar g cca Pr o c ac Hi s gt c ValI 120 ac c Thr ct g Le u ct c Le u at t I Ie gca Al a gt t ValI ct g Le u t ac Tyr C ac Hi s gag O31u g aa O31u 1 05 cgt Ar g aaa Ly s gag O31u gag O31u g aa O31u 1 85 tt g Le u ggC G31y at g vb t ac c Thr g ac As p 75 t cc Ser gca Al a gca Al a at c I Ie cca Pr o 1 55 ccc Pr o ct g Le u C ac Hi s ct t Le u C ag O31n gcc Al a aac As n ac c Thr gcg Al a ggc G31y t gt cys 140 gca Al a cgc Ar g ct c Le u t cc Ser gt g ValI ggc G31y cgt Ar g gca Al a gt g ValI aag Ly s ggc G31y 125 ct g Le u ac c Thr g ac As p gca Al a ac c Thr gt t ValI ggc G31y ggc G31y g aa (31u ct c Le u C ag O31n 110 g aa O31u gcc Al a at g vb t ct c Le u g at As p 1 ac c Thr ac c Thr at c I Ie ct c Le u aag Ly s t ac Tyr at g vb t g aa O31u gag O31u ac c Thr gt g ValI 1 gt c ValI aag Ly s aac As n gga O31y aag Ly s at c I Ie cca Pr a aag Ly s gca Al a t cc Ser C ac Hi s 1 cgc Ar g gag (31u aat aac ct t Asn Asn Leu t agaaact at t tggcggcaa gca <210O> 194 <211> 198 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn Page 248 seql i st cor r ect ed. t xt <400> 194 Sn Pro Leu Lys Leu y Ala His Ala Val Leu His Ser Thr Thr Lys 1 Tyr Asp Pr o Thr Al a Gy Ar g Al a Leu 145 G n lie Gn Gy Gu Pr o Al a Phe Lys Gy 115 Lys Gy Al a SIle Leu 195 Hi s Asp Val Ar g Asp Hi s bet Cys G u Gy 165 I le Asn Asp Gu Asp Asp Ar g Gy Ser Ser 135 Leu G n Asp Val Leu Tyr Hi s G u G u 105 Ar g Lys G u G u G u 185 Gy bet Thr Asp Ser Al a Al a SIle Pr o 155 Pr o Leu Leu Gn Al a Asn Thr Al a Gy Cys 140 Al a Ar g Leu Val Gy Ar g Al a Val Lys Gy 125 Leu Thr Asp Al a Val Gy Gy G u Leu G n 110 G u Al a et Leu Asp 190 Thr SIe Leu Lys Tyr bet G u G u Thr Val 175 Val <210> 195 <211> 1170 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1147) <223> RXA02532 <400> 195 gatgaatttt tacccaccat ctgtacctat taaccctgcg tggcgtccac ccacagtaac tgtgcaagcg ggacggccag ccagaactcc tggtgcgccg atg aac cca cct atc IVet Asn Pro Pro Ile 1 acg ttg tcc agc act tat gtt cat gat tca gaa aaa gct tat ggg cgc Thr Leu Ser Ser Thr Tyr Val His Asp Ser G u Lys Ala Tyr G y Arg 15 gat ggc aat gat gga tgg ggt gca ttt gag gct gcc atg gga act cta Asp y Asn Asp y Trp y Ala Phe u Ala Ala IVet y Thr Leu 30 gat ggt ggg ttc gcg gta tct tat tct tca ggt ttg gca gcg gca acg Asp y y Phe Ala Val Ser Tyr Ser Ser y Leu Ala Ala Ala Thr Page 249 seql i st cor r ect ed. t xt 45 tcg att gct gat ttg gtt cct act ggt ggc aca gtt gtt tta cct aaa 307 Ser lie Ala Asp Leu Val Pro Thr Gy y Thr Val Val Leu Pro Lys 60 gct gcc tat tat ggc gtg acc aat att ttc gcc agg atg gaa gcc cgc 355 Ala Ala Tyr Tyr y Val Thr Asn Ile Phe Ala Arg Iet G u Ala Arg 75 80 gga agg ctg aag gtt cga act gtt gat gca gac aat acc gaa gaa gtg 403 Sy Arg Leu Lys Val Arg Thr Val Asp Ala Asp Asn Thr G u G u Val 95 100 att gct gct gct caa ggt gca gat gtg gtg tgg gtg gaa tcg atc gct 451 Ile Ala Ala Al a Gn Gy Ala Asp Val Val Trp Val Gu Ser Ile Ala 105 110 115 aat ccg acg atg gtg gta gct gat atc cct gca ata gtc gac ggt gtg 499 Asn Pro Thr IVet Val Val Ala Asp lie Pro Ala lie Val Asp y Val 120 125 130 cgt ggg ctt gga gtt ttg act gtc gtt gac gcg act ttc gca acg cca 547 Arg y Leu y Val Leu Thr Val Val Asp Ala Thr Phe Ala Thr Pro 135 140 145 ctt cgt caa cgt cca ttg gaa ctt ggt gct gat att gtg ctt tac tcg 595 Leu Arg G n Arg Pro Leu G u Leu Gy Ala Asp lie Val Leu Tyr Ser 150 155 160 165 gca acc aaa ctt atc ggt gga cac tct gat ctt ctt ctt gga gtc gca 643 Ala Thr Lys Leu lie G y G y His Ser Asp Leu Leu Leu G y Val Ala 170 175 180 gtg tgc aag tct gag cac cat gcg cag ttt ctt gcc act cac cgt cat 691 Val Cys Lys Ser G u His His Ala G n Phe Leu Ala Thr His Arg His 185 190 195 gat cat ggt tca gtg ccg gga ggt ctt gaa gcg ttt ctt gct ctc cgt 739 Asp His G y Ser Val Pro G y G y Leu G u Ala Phe Leu Ala Leu Arg 200 205 210 gga ttg tat tcc ttg gcg gtg cgt ctt gat cga gca gaa tcc aac gca 787 Sy Leu Tyr Ser Leu Al a Val Arg Leu Asp Arg Ala u Ser Asn Ala 215 220 225 gca gaa ctt tcg cgg cga ctt aac gcg cat cct tcg gtt acc cgc gtc 835 Ala G u Leu Ser Arg Arg Leu Asn Ala His Pro Ser Val Thr Arg Val 230 235 240 245 aat tat cca gga ctt cct gat gat ccc caa cat gaa aaa gcc gtg cga 883 Asn Tyr Pro G y Leu Pro Asp Asp Pro G n His G u Lys Ala Val Arg 250 255 260 gtc cta ccc tct gga tgt gga aac atg ttg tca ttt gag ctt gat gca 931 Val Leu Pro Ser y Cys y Asn IVet Leu Ser Phe G u Leu Asp Ala 265 270 275 aca cct gaa cga act gat gag att ctc gaa agc ctg tca ctt tta acc 979 Thr Pro G u Arg Thr Asp G u Ile Leu u Ser Leu Ser Leu Leu Thr 280 285 290 cac gcg acc agt tgg gga ggt gtg gaa aca gcc att gaa cgt cgc acc 1027 His Ala Thr Ser Trp G y G y Val G u Thr Ala I Ie G u Arg Arg Thr 295 300 305 agg cgg gat gct gaa gtg gtg gca gaa gta ccg atg act ctt tgc cgc 1075 Arg Arg Asp Ala G u Val Val Ala G u Val Pro Iet Thr Leu Cys Arg Page 250 310 gt t t cc gt a gga at t Val Ser Val G31y I Ie 330 gcc tca atc gac aaa Ala Ser I Ie Asp Lys 345 315 gaa (31u gt t ValI seqI i st car r ect ed. t xt 320 325 gt t gaa gat ct a tgg gaa gac ctc aac Val G31u Asp Leu Trp G31u Asp Leu Asn 335 340 ggt t agaact cgt agccagt aac cag G31y 1123 1170 <210O> 196 <21 1> 349 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 196 I'bt Asn Pro Pro lieThr Ly s A a Le u ValI Ar g As n ValI I Ie Thr 145 li1e Le u A a Ph e A a 225 Ser (31u Tyr G31y A a Le u G31u G31u Ser 115 As p A a Le u G31y Hi s 1 95 A a Ser Thr A a Ar g Le u Thr Ly s Ar g ValI A a ValI Pr a Ser 1 65 A a Hi s Ar g A a ValI 245 Ar g As p As p Ser A a 70 G31y I Ie As n Ar g Le u 150 A a ValI As p G31y A a 230 As n ValI Leu Ser Ser Thr 10 G31yAs nAsp (1y G3 y G3 y Ph e A a 40 Il e Al aAsp Leu A a Ty r Ty r G3 y Arg Leu Lys Val 90 A a A a A a G3 n 1 05 Pr o Thr Nib t Val 120 a3 y Leu a3 y Val 135 Ar g a31n Ar g Pr o Thr Lys Leu Ilie 1 70 Cys Lys Ser a3 u 1 85 Hi s a3 y Ser Val 200 Leu Tyr Ser Leu 215 a31u Leu Ser Ar g Tyr Pr o a31y Leu 250 Leu Pr o Ser a31y 265 Tyr Tr p ValI ValI ValI 75 Ar g a31y ValI Le u Le u 1 55 a31y Hi s Pr a A a Ar g 235 Pr a ValI a31y Ser Pr a Thr Thr A a A a Thr 140 a31u a31y Hi s a31y ValI 220 Le u As p Hi s A a Tyr Thr As n ValI As p As p 125 ValI Le u Hi s A a a31y 205 Ar g As n As p As p Ph e Ser ay le As p ValI 110 I Ie ValI a31y Ser a31n 1 Le u Le u A a Pr a Ser a31u Ser a31y Ph e A a ValI Pr a As p A a As p 1 Ph e a31u As p Hi s a31n 255 a31u A a a31y Thr A a As p Tr p A a A a As p 1 Le u Le u A a Ar g Pr a 240 Hi s Cys a31y Asn I'bt Leu Ser 270 Page 251 seql i st cor r ect ed. t xt Phe G u Leu Asp Ala Thr Pro u Arg Thr Asp u Ile Leu u Ser 275 280 285 Leu Ser Leu Leu Thr His Ala Thr Ser Trp G y G y Val G u Thr Ala 290 295 300 lie G u Arg Arg Thr Arg Arg Asp Ala G u Val Val Ala G u Val Pro 305 310 315 320 IVet Thr Leu Cys Arg Val Ser Val G y Ile G u Asp Val G u Asp Leu 325 330 335 Trp G u Asp Leu Asn Al a Ser Ile Asp Lys Val Leu y 340 345 <210> 197 <211> 861 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(838) <223> RXS03159 <400> 197 aggggctagt tttacacaaa agtggacagc ttggtctatc attgccagaa gaccggtcct tttagggcca tagaattctg attacaggag ttgatctacc ttg tct aac Asn gac Asp ttc Phe cgt Ar g ct c Leu acc Thr ct c Leu ttc Phe cag G n t ac Tyr cag G n ggc G y ggc G y at c SIle aac Asn gca Al a 120 ttc Phe ggt Gy gct Al a ccc Pr o aag Lys ttc Phe gct Al a ggc G y t cc Ser tcg Ser cca Pr o acc Thr t at Ty r 75 cgc Ar g tac Tyr gt c Val act Thr at t SIle aac Asn at c SIle ggc G y at c SIle ggc G y gaa G u gca Al a aac Asn gaa G u 45 gt g Val cgc Ar g at c SIle gga G y t ac Tyr 125 tcg Ser acc Thr ct g Leu gca Al a gca Al a ct c Leu acc Thr 110 acc Thr at t I e 15 cca Pr o cgc Ar g tta Leu ttc Phe aag Lys 95 t ac Tyr gtt Val cac Hi s at c SIle aaa Lys gag G u t cc Ser 80 ccg Pr o cgc Ar g gtt Val Leu Ser 1 gct ggg Al a G y tat gcc Tyr Al a ggc tac G y Tyr cag acc G n Thr t cc ggc Ser G y ggc gat Sy Asp ct g at c Leu II e gat acc Asp Thr 130 ttt gac cca Phe Asp Pro tat gag cca Tyr G u Pro t cc acc acc Ser Thr Thr gag tac acc G u Tyr Thr gtc gca gca Val Al a Al a atg gct gca bet Al a Al a cac at c gt c Hi s I I e Val 100 gac acc gta Asp Thr Val 115 tcc gtc gtg Ser Val Val gaa gag gtc aag gca gcg atc aag gac aac acc G u G u Val Lys Ala Ala lle Lys Asp Asn Thr ctg atc tgg gtg Leu Ile Trp Val Page 252 gaa G u 150 gca Al a ttg Leu agt Ser ttg Leu tgt Cys 230 acc Thr seql i st cor r ect ed. t xt acc cca acc aac cca gca ctt ggc atc acc gac atc Thr Pro Thr Asn Pro Ala Leu Gy Ile Thr Asp Ile 155 160 aag ctc acc gaa ggc acc aac gcc aag ttg gtt gtt Lys Leu Thr G u G y Thr Asn Ala Lys Leu Val Val 170 175 gca tcc cca tac ctg cag cag cca cta aaa ctc ggc Ala Ser Pro Tyr Leu n G n Pro Leu Lys Leu y 185 190 cct tgc act cca cca cca agt aca tcg aag gac act Pro Cys Thr Pro Pro Pro Ser Thr Ser Lys Asp Thr 200 205 210 gcg gcc ttg tgg gta cca acg acc agg aaa tgg acg Ala Ala Leu Trp Val Pro Thr Thr Arg Lys Trp Thr 215 220 225 tca tgc agg gcg gca tcg gac cga tcc cat cag ttt Ser Cys Arg Ala Al a Ser Asp Arg Ser His G n Phe 235 240 tgaccgcccg tggcctcaag acc gaa G u gac Asp gca Al a 195 ccg Pr o aag Lys tcg Ser gca Al a aac Asn 180 cac Hi s acg Thr aac Asn at g Met <210> 198 <211> 246 <212> PRT <213> Corynebacter i um gl ut ari cum Leu 1 Al a Tyr Gy G n Ser Sy Leu Asp Lys 145 0> 198 Ser Phe Asp G y Tyr G u Al a Ser Thr Tyr G u Tyr Thr Val Al a G y MVt Ala Asp His Ile 100 Ile Asp Thr 115 Thr Ser Val 130 Leu Ile Trp Pro Asn Thr 5 Pro Asp Asp Thr Phe Al a Thr Arg Val 55 Ala Leu G u 70 Al a Thr Asp Val Leu G y Val Phe Thr Val u u 135 Val G u Thr 150 Sn G y Phe 10 Tyr Tyr G y 25 G n Asn Ala 40 Sy Asn Pro G y Al a Lys I e Leu Phe 90 Asn Asp Al a 105 Al a Trp G y 120 Val Lys Al a Pro Thr Asn Ser Ser Pr o Thr Tyr 75 Ar g Tyr Val Al a Pr o 155 Thr SIle Asn SIle Sy SIle Sy G u l Ie 140 Al a Al a Asn G u Val Ar g SIle Sy Tyr 125 Lys Leu Ser Thr Leu Al a Al a Leu Thr 110 Thr Asp Sy Ile His Pro Ile Arg Lys Leu G u Phe Ser Lys Pro Tyr Arg Val Val Asn Thr Ile Thr 160 Lys Leu 175 Asp Ile u Ala Val 165 Al a Lys Leu Thr G u y Thr Asn Al a 170 Page 253 seql i st cor r ect ed. t xt Val Val Asp Asn Thr Leu Ala Ser Pro Tyr Leu n n Pro Leu Lys 180 185 190 Leu G y Ala His Ala Ser Pro Cys Thr Pro Pro Pro Ser Thr Ser Lys 195 200 205 Asp Thr Pro Thr Leu Leu Ala Ala Leu Trp Val Pro Thr Thr Arg Lys 210 215 220 Tr p 225 Thr Lys Asn Cys Cys Ser Cys Arg Ala Ala Ser Asp Arg Ser His 230 235 240 G n Phe Ser Mbet Hi s Thr 245 <210> <211> <212> <213> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> <223> <220> <221> <222> <223> 199 703 DNA Corynebact eri CDS (703) FRXA02768 um gl ut am cum i sc feature 676, 687, 690, 691, n a, c, g, or t VARI ANT 192 Xaa His or G VARI ANT 196 Xaa Ile, Thr, Asn, or Ser VARI ANT 197 Xaa Phe, Leu, Ser, Trp, or STOP CODCN VARI ANT 198 Xaa Val, Ala, G u, Tyr, Cys, or G y <400> 199 aggggctagt tttacacaaa agtggacagc ttggtctatc attgccagaa gaccggtcct tttagggcca tagaattctg attacaggag ttgatctacc ttg tct ttt gac cca Leu Ser Phe Asp Pro 1 aac acc cag ggt Asn Thr G n G y ttc tcc act gca tcg att Phe Ser Thr Ala Ser Ile 15 cac gct ggg tat His Al a y Tyr gag cca G u Pro gac gac tac Asp Asp Tyr tac ggt tcg att aac acc Tyr Gy Ser Ile Asn Thr 30 cca atc tat gcc Pro Ile Tyr Ala tcc acc acc Ser Thr Thr Page 254 ttc gcg cag Phe Al a G n aac gct cca aac Asn Ala Pro Asn ga 4 4 seql i st cor r ect ed. t xt a ctg cgc aaa ggc ta u Leu Arg Lys Gy Ty c r 0 gag tac acc G u Tyr Thr 5 5 cgt gtg Arg Val ggc aac ccc acc G y Asn Pro Thr at c I e gtg gca tta gag Val Ala Leu G u acc gtc gca gca Thr Val Ala Ala ct c Leu gaa ggc gca aag Gu y Ala Lys ggc cgc gca ttc Sy Arg Ala Phe t cc Ser 80 t cc ggc atg gct Ser G y Met Al a gca Al a acc gac atc ctg Thr Asp Ile Leu ttc Phe cgc atc atc ctc Arg Ile Ile Leu ccg ggc gat cac Pro G y Asp Hi s at c gt c Ile Val 100 ctc ggc aac Leu G y Asn gct tac ggc gga Al a Tyr G y G y acc Thr 110 tac cgc ctg at c Tyr Arg Leu Ile gac acc gta Asp Thr Val 115 tcc gtc gtg Ser Val Val ttc acc gca tgg ggc gtc gaa tac acc gtt gtt gat acc Phe Thr Ala Trp y Val u Tyr Thr Val Val Asp Thr 120 125 130 gaa gag Gu Gu 135 gtc aag gca gcg Val Lys Al a Al a at c l Ie 140 aag gac aac acc Lys Asp Asn Thr gct gat ctt ggt Al a Asp Leu G y gga Sy 150 aac ccc aac caa Asn Pro Asn G n agc act ttg gca Ser Thr Leu Al a tta Leu 160 ccc gac atc gaa Pro Asp Ile u gca Al a 165 gtn tgc aaa act Val Cys Lys Thr t ca Ser 170 ccc gaa agg cac Pro G u Arg His caa G n 175 ccc caa gct tgt Pro n Ala Cys tgt ttg Cys Leu 180 aca aca cct Thr Thr Pro cat tcc cca tac His Ser Pro Tyr ct g Leu 190 cag can cca ctt G n Xaa Pro Leu aaa ant tnn Lys Xaa Xaa 195 gng cac acg cag 703 Xaa His Thr G n 200 <210> <211> <212> <213> <220> <221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <223> <223> 200 201 PRT Corynebact er i um gl ut am cum VARI ANT 192 Xaa His or G n VARI ANT 196 Xaa Ile, Thr, Asn, VARI ANT 197 Xaa Phe, Leu, Ser, Trp, or STOP CODCN or Ser Tyr, Cys, <220> <221> VARIANT Page 255 seql i st cor r ect ed. t xt <222> 198 <223> Xaa Val, Ala, Gu, or G y <400> 200 Leu Ser Phe 1 Al a G y Tyr Tyr Al a Ser G y Tyr G u G n Thr Val Ser G y M et G y Asp Hi s Leu Ile Asp 115 Asp Thr Ser 130 Lys Al a Asp 145 Pro Asp I I e G n Al a Cys Pro Leu Lys 195 Pro Asn Thr 5 Pr o Thr Thr Al a Al a Val Val Val Sy Al a 165 Leu Asp Al a Val G u Asp Sy Thr G u 135 Asn Cys Thr Sn G y Phe 10 Tyr Tyr G y G n Asn Al a 40 Sy Asn Pro G y Al a Lys I e Leu Phe 90 Asn Asp Al a 105 Al a Trp G y 120 Val Lys Al a Pro Asn n Lys Thr Ser 170 Pro Ser His 185 Ser Ser Pr o Thr Tyr Ar g Tyr Val Al a Pr o 155 Pr o Ser Al a Asn G u Val Ar g SIle Sy Tyr 125 Lys Thr Ar g Tyr Ser Thr Leu Al a Al a Leu Thr 110 Thr Asp Leu Hi s Leu 190 I e Pr o Ar g Leu Phe Lys Tyr Val Asn Al a G n 175 G n Xaa Xaa Xaa His Thr G n 200 <210> 201 <211> 1113 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1090) <223> RXA00216 <400> 201 gtgt t gctcg cggccaggca gcagtgctgt acctgcctga cgcggatggt gacatcgt t c ttggatcagg caccatctgc cacacggagt ct t aagaaaa ttg ggc gct tat ggt 115 Leu G y Al a Tyr G y 1 tta ggt gag ctt cct gga aaa tcc gcc gcg gaa gcc gcc gac att att 163 Leu G y G u Leu Pro G y Lys Ser Ala Ala G u Ala Ala Asp Ile Ile 15 cag ggt gaa acg ggc gat ctt ctc cat att cct cag ctt ccg gcg cga 211 Gn G y u Thr Gy Asp Leu Leu His lie Pro n Leu Pro Ala Arg 30 Page 256 seql i st cor r ect ed. t xt ggt Gy at c SIle agc Ser gcg Al a gtt Val cat Hi s ct g Leu cgc Ar g 150 at c SIle gt g Val at t SIle gat Asp caa G n 230 agt Ser gat Asp ttt Phe gat Asp ttg ggt Leu G y aac gtt Asn Val aga ttg Arg Leu tgc gag Cys u gct ggt Al a G y cgc gtt Arg Val 120 atc gcc I e Al a 135 gcc gaa Al a G u gac ggc Asp y aat gt c Asn Val gag ggg G u Gy 200 gtg gct Val Ala 215 gtc cgt Val Arg gga att G y lle gaa ct g G u Leu ttt gat Phe Asp 280 att cat Ile Hi s 295 gct gat Al a Asp gat cgc Asp Arg acg cac Thr His gaa acc G u Thr ccc tgg Pro Trp 105 ttg tct Leu Ser ggc at c G y lle gtg cag Val G n t cc ct c Ser Leu 170 gct gat Al a Asp 185 ccg aca Pro Thr cgg ggt Arg G y gga aat G y Asn cgt ctt Arg Leu 250 ctc gag Leu G u 265 cgt tta Arg Leu ccg ggt Pro G y ct g at c Leu Il e ggg G y ct g Leu 75 tgg Tr p act Thr gat Asp gat Asp gt g Val 155 cct Pr o gcc Al a t at Ty r gcg Al a gaa G u 235 ggt G y cga Ar g ggt G y gag G u gcc Al a acc Thr gga G y tta Leu cgc Ar g gcg Al a 140 caa G n ggc G y agt Ser ct c Leu ggc G y 220 cat Hi s ttg Leu ccg Pr o gt g Val gat Asp 300 ggt G y 45 cga Ar g ggc G y acg Thr ggt G y ggt G y 125 cat Hi s at t SIle act Thr gaa G u aac Asn 205 gcc Al a ttg Leu ggc G y cgg Ar g ggc G y 285 ttg Leu cga acc gtc ggt Arg Thr Val y t ct Ser gat Asp ggc G y gcg Al a 110 gcg Al a gca Al a gat Asp t cc Ser cgt Ar g 190 ct c Leu gat Asp gat Asp at t SIle caa G n 270 cga Ar g gt g Val gt g Val ctt Leu 80 gac Asp at t l Ie cgt Ar g aag Lys ccg Pr o 160 ttt Phe cag G n ggc G y gt g Val ttt Phe 240 aca Thr gcc Al a t at Tyr ctg ctg gac Leu Leu Asp agc aca cgc Ser Thr Arg atg gat ttg IVet Asp Leu ct a aaa at c Leu Lys II e 100 ttg gcc aat Leu Al a Asn 115 ctc acg cag Leu Thr G n 130 gct ggg cga Al a G y Ar g ct g aaa t cg Leu Lys Ser att att cct Ile Ile Pro 180 gtc ttt agc Val Phe Ser 195 att cct act I e Pro Thr 210 att tcc atg Ile Ser IVet gaa acc at c G u Thr lie aaa gat gt c Lys Asp Val 260 gag gta gca Su Val Ala 275 gtg gat gct Val Asp Al a 290 at g Met ccc Pr o gat Asp caa G n ggc G y gcg Al a ttt Phe ctt Leu 165 gcg Al a tcg Ser tgg Tr p gat Asp acc Thr 245 gt a Val cgc Ar g gtt Val 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 cag ggg acc atc acc gag gcc Sn Gy Thr Ile Thr u Ala 305 Page 257 seql i st cor r ect ed. t xt gcg cag gct tat cgc atg gcc cgg gtg atg tcg gag atg ttg tcg aag Ala n Ala Tyr Arg IVet Ala Arg Val IVet Ser G u IVet Leu Ser Lys 310 315 320 325 gat tca tgc gac ctt taaggcttta ccggcgctgg gtg Asp Ser Cys Asp Leu 330 <210> 202 <211> 330 <212> PRT <213> Cor ynebact eri um gl ut am cum 1075 1113 <400> 202 Leu G y Al a 1 Al a G n Gy let Asp Lys G u Asp Val 145 G u Asp G n G n G n 225 Gy Gy Asp Pr o Leu Thr Asp Lys Al a 115 Thr Gy Lys SIle Phe 195 Pr o Ser Thr Asp Tyr G y 5 Ile Ile Al a Ar g Asp IVet Arg Pro Leu Asp Ile G n 100 Asn y G n Al a Arg Phe Ser Leu 165 Pro Al a 180 Ser Ser Thr Trp IVet Asp Ile Thr 245 Val Val 260 Sy Leu Asn Ar g Cys Al a Ar g l Ie 135 Al a Asp Asn G u Val 215 Val Sy G u Leu G y G u Leu Thr 25 Al a Asp Thr G u Pr o 105 Leu Sy Val Ser Al a 185 Pr o Ar g Sy Ar g Leu 265 Sy Asp Leu Gy Leu Tr p Thr Asp Asp Val 155 Pr o Al a Tyr Al a G u 235 G y Ar g Lys Leu SIle Al a Thr Sy Leu Ar g Al a 140 G n Sy Ser Leu Sy 220 Hi s Leu Pr o Ser Leu Sy Ar g Sy Thr Sy Sy 125 Hi s SIle Thr G u Asn 205 Al a Leu G y Ar g Al a Hi s Ar g Ser Asp Sy Al a 110 Al a Al a Asp Ser Ar g 190 Leu Asp Asp SIle G n 270 Al a I e Thr Tr p Phe Val Ar g let Ar g G u Thr 175 Leu Thr Thr Sy Thr 255 Lys Val G u Val Ala Arg Phe Phe Asp Arg Leu y Val y Arg Asn Tyr Page 258 275 Leu Val Asp Ala Val 290 Thr lie Thr u Ala 305 G u IVet Leu Ser Lys 325 seql i st cor r ect ed. t xt 280 285 Asp lie His Pro G y u Asp Leu Val n y 295 300 Al a G n Ala Tyr Arg Iet Ala Arg Val Iet Ser 310 315 320 Asp Ser Cys Asp Leu 330 <210> 203 <211> 623 <212> DNA <213> Corynebacteri um gl ut am cum <220> <221> CDS <222> <223> RXN00402 <400> 203 act Thr 1 gat Asp ttc Phe ct c Leu t cc Ser ttc Phe aag Lys acc Thr tgg Tr p ggc Gy 145 cag G n gaa G u gct Al a ct c Leu gca Al a cgc Ar g aac Asn t cc Ser t cc Ser 115 ttt Phe gtt Val gac Asp gat gga aag Asp G y Lys 5 cac gga ttg Hi s G y Leu gtt cgc gtt Val Arg Val aac gca tgg Asn Al a Tr p gag cgc cac G u Ar g Hi s 70 cac gag aag Hi s G u Lys tgg tac gca Trp Tyr Al a ctc acc ttc Leu Thr Phe gac gcc ctg Asp Al a Leu 135 tcc ctc gtt Ser Leu Val 150 gct ggc ctg Al a G y Leu 165 cca gt a Pro Val aag Lys ggc G y 40 gct Al a aac Asn gt g Val acc Thr gag G u 120 aag Lys gtt Val gca Al a t ac Tyr ctt Leu gca Al a gaa G u gaa G u aag Lys 105 at c SIle ct a Leu cac Hi s cgc Ar g ttg Leu 10 gca Al a ct a Leu gt c Val aac Asn aag Lys 90 gaa G u aag Lys cac Hi s cca Pr o gcg Al a 170 ccc tac ttc gtc Pro Tyr Phe Val act cca Thr Pro gac Asp cgc Ar g cag G n gcc Al a 75 gtt Val aag Lys ggc Gy t cc Ser gca Al a 155 ggc Gy ggt G y acc Thr at c SIle aag Lys ttc Phe ggc Gy aag Lys 125 ctt Leu acc Thr acc Thr gca Al a ggc G y gac Asp gtt Val gca Al a ct g Leu 110 gat Asp gca Al a acc Thr cag G n cca Pr o t cc Ser acc Thr gca Al a ggc G y aag Lys gag G u aac Asn cat Hi s t cc Ser 175 gcc Al a acc Thr ctt Leu gaa G u ctg Leu t ac Tyr gct Al a at c SIe t ca Ser 160 acc Thr gtc cgc ctg tcc gtt ggc atc gag acc att gat gat atc atc gct gac Page 259 seql i st cor r ect ed. t xt Val Arg Leu Ser Val Gy Ile Gu Thr Ile Asp Asp lie lie Ala Asp 180 185 190 ctc gaa ggc ggc ttt gct gca atc tagctttaaa tagactcacc cca Leu G u G y G y Phe Ala Ala lle 195 200 <210> 204 <211> 200 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 204 Thr 1 Asp Phe Leu Ser Phe Lys Thr Tr p Gy 145 G n Val Leu Asp G u Lys Al a Leu Al a Ar g Asn Ser Ser 115 Phe Val Asp Leu Sy 195 Asp 5 Hi s Val Asn G u Hi s Tr p Leu Asp Ser Al a 165 Val Lys Leu Val Tr p Hi s Lys Al a Phe Leu 135 Val Leu SIle Pr o Lys Sy 40 Al a Asn Val Thr G u 120 Lys Val Al a G u Val Tyr Leu Al a G u G u Lys 105 I le Leu Hi s Ar g Thr 185 Leu 10 Al a Leu Val Asn Lys 90 G u Lys Hi s Pr o Al a 170 I le Pr o Asp Ar g G n Al a 75 Val Lys Sy Ser Al a 155 Sy Asp Phe Sy Thr I le Lys Phe Sy Lys 125 Leu Thr Thr SIle Val Al a Sy Asp Val Al a Leu 110 Asp Al a Thr G n SIle 190 Thr Pr o Ser Thr Al a Sy Lys G u Asn Hi s Ser 175 Al a Pr o Al a Thr Leu G u Leu Tyr Al a SIe Ser 160 Thr Asp Sy Phe Ala Ala I le <210> 205 <211> 599 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA00402 <400> 205 gta ttg ccc tac ttc gtc act cca gat gct gct tac cac gga ttg aag Val Leu Pro Tyr Phe Val Thr Pro Asp Ala Ala Tyr His G y Leu Lys 1 5 10 Page 260 seql i st cor r ect ed. t xt tac gca gac ctt ggt gca Tyr Ala Asp Leu y Al a ctt Leu gca Al a gaa G u gaa G u aag Lys at c SIle ct a Leu cac Hi s 145 cgc Ar g acc Thr cgc Ar g cag G n gcc Al a gtt Val aag Lys ggc Gy 115 t cc Ser gca Al a ggc Gy gat Asp acc Thr at c SIle aag Lys ttc Phe ggc G y aag Lys ctt Leu acc Thr acc Thr 165 at c SIle ggc G y gac Asp gtt Val 70 gca Al a ct g Leu gat Asp gca Al a acc Thr 150 cag G n at c SIle cca Pr o t cc Ser acc Thr gca Al a ggc G y aag Lys gag G u aac Asn 135 cat Hi s t cc Ser gct Al a Al a Phe 25 acc ct c Thr Leu 40 ctt tcc Leu Ser gaa ttc G u Phe ctg aag Leu Lys tac acc Tyr Thr 105 gct tgg Al a Tr p 120 atc ggc I e G y t ca cag Ser G n acc gt c Thr Val gac ct c Asp Leu 185 t cc Ser ct g Leu ct c Leu gat Asp 90 ggc G y gca Al a gat Asp t cc Ser cgc Ar g 170 gcc ttc gca Al a cgc Ar g aac Asn 75 t cc Ser t cc Ser ttt Phe gtt Val gac Asp 155 ct g Leu ggc ctc aag gtt G y Leu Lys Val aac Asn gag G u cac Hi s tgg Tr p ct c Leu gac Asp 125 t cc Ser gct Al a gtt Val cgc gtt ggc Arg Val y gca t gg gct Al a Trp Al a cgc cac aac Arg His Asn gag aag gtg G u Lys Val tac gca acc Tyr Al a Thr acc tt c gag Thr Phe G u 110 gcc ctg aag Ala Leu Lys ctc gtt gtt Leu Val Val ggc ctg gca G y Leu Al a 160 ggc atc gag G y lie u 175 gct gca at c Al a Al a le 190 gaa ggc ggc ttt G u G y G y Phe t agct t t aaa t agact cacc cca <210> 206 <211> 192 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 206 Val Leu Pro Tyr Phe Val Thr Pro Asp 1 5 Tyr Ala Asp Leu Gy Ala Pro Ala Phe 25 Leu Leu Arg Asp Thr G y Ser Thr Leu 40 Ala Val n Gy I e Asp Thr Leu Ser 55 G u Asn Ala lie Lys Val Ala G u Phe 70 Ala Ala Tyr His Gy Leu Lys 10 Sy Leu Lys Val Arg Val y Ser Ala Phe Asn Ala Trp Ala Leu Arg Leu G u Arg His Asn Leu Asn Asn His G u Lys Val 75 Page 261 O31u Ly s I Ie Le u Hi s 145 Ar g Thr ValI Ly s G31y 115 Ser A a G31y As p Ph e G31y Ly s Le u Thr Thr 1 65 li1e A a Le u As p A a Thr 150 O31n SI e G31y Ly s O31u As n 135 Hi s Ser A a seql i st car r ect ed. t xt Leu Lys Asp Ser Pro Trp Ty r Thr 03 y Se r Val Leu 1 05 Ala Tr p Ala Phe I Ie As p 120 125 li1e G31y As p Val Ar g Ser 140 Ser 03 n Ser As p 03 u A a 1 55 Thr Val Ar g Leu Ser Val 1 As p Leu 03 u 03 y 03 y Phe 1 85 Ty r Th r 110 A a Le u G31y G31y A a 1 Th r O31u Ly s ValI A a 1 Ou le <210O> 207 <21 1> 613 <212> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> 101). (613) <223> RXA00405 <400> 207 agaat aaat t t at accacac agt ctat tgc aat agaccaa ggagaagaat t tcct aat aa aaact ct taa ggacct ccaa gct gt t cagt agggt gcat g cca aag tac Pro Lys Tyr aat As n ggC O31y c aa O31n tt c Ph e ac c Thr c ac Hi s aat As n t ca Ser ac c Thr ct t Le u gag O31u gt a ValI g ac As p g ac As p tt c Ph e g at As p tt g Le u tt c Ph e cag O31n gca A a gt g ValI ct a Le u gaa (31u 75 t cc Ser t gg Tr p c ag O31n tt c Ph e ggC O31y aac As n t cc Ser ggC O31y ac c Thr g ac As p 45 cct Pr o cgc Ar g gga (31y tt t Ph e ag c Ser t cc Ser gt t ValI at c I Ie c ag O31n g aa O31u 15 gca A a gct A a t ac Tyr gct A a gcc A a 95 ac c Thr cga Ar g gag O31u t cc Ser t cc Ser gca A a t cc Ser ct t Le u gcc A a ct c Le u g aa O31u ac c Thr c ac Hi s at c I Ie c ag O31n aac As n ggc O31y gcc A a 1 00 ttg aac ctg gca gga gcg ggc gac cac atc gtc acc tcc cca cgc ctc Page 262 Leu t ac Tyr at c I le gca Al a 150 aac Asn Leu ggc G y 120 gtt Val gtt Val cag G n Gy gag G u ttc Phe cca Pr o gac Asp 170 Al a act Thr gt g Val aac Asn 155 gt c Val seql i st cor r ect ed. t xt G y Asp His I I e Val Thr Ser Pro Arg Leu 110 115 cta ttc ctt atc act ctt aac cgc ctg ggt Leu Phe Leu lie Thr Leu Asn Arg Leu y 125 130 gaa aac ccc gac gac cct gag tcc tgg cag G u Asn Pro Asp Asp Pro G u Ser Trp G n 140 145 acc aaa gca ttc ttc ggc gag act ttc gcc Thr Lys Ala Phe Phe y G u Thr Phe Ala 160 165 <210> 208 <211> 171 <212> PRT <213> Corynebacter i um gl ut am cum <400> 208 lIet Pro Lys 1 Arg Ser II e Asn Leu Pro Hi s Al a Lys Arg Leu Thr Leu G u G y Thr Thr Asn Thr Ser Pro 115 Leu Asn Arg 130 Pro u Ser 145 G y G u Thr <210> 209 <211> 551 <212> DNA Tyr Asp Asn Ser 5 His Al a y G n Ile Tyr G n Ser G n Arg Phe Ala Asn Pro Thr Val 70 Sy Val His Ala Ala l e Leu Asn 100 Arg Leu Tyr y Leu G y I e Asp 135 Trp G n Al a Ala 150 Phe Ala Asn Pro 165 Asn Al a Asp 10 Ser Val Asp Thr Al a Phe 40 Leu G u Asp G u Al a Leu Val Al a Phe 90 Leu Al a G y 105 G y Thr G u 120 Val Ser Phe Val n Pro G n Al a Asp 170 G n Al a Val Leu G u 75 Ser Al a Thr Val Asn 155 Val Sy Thr Asp Pr o Ar g Sy Asp Phe 125 Asn Lys G u Al a Al a Tyr Al a Al a I e I e Asp Phe Thr Ar g G u Ser Ser Al a Val Thr Asp Phe 160 <213> <220> <221> <222> Cor ynebact eri um gl ut ai cum CDS (528) Page 263 seqI i st car r ect ed. t xt <223> RXA02197 <400> 209 gcc A a Le u G31y at t I Ie t ac Tyr gag O31u gga G31y ct g Le u g at As p at c I Ie 145 ac a Thr cgc Ar g at c I Ie gt g ValI g at As p g aa O31u t gg Tr p gt c ValI t ac Tyr 115 g aa O31u gt g ValI gcg A a cgc Ar g g at As p gt c ValI gcc A a c ac Hi s t cc Ser g at As p ggc G31y cgc Ar g tt g Le u gt g ValI 1 65 tt t Ph e t t c Ph e at g vb t aac As n ggc G31y 70 cga Ar g tt t Ph e gcc A a gca A a t cc Ser 150 ct c Le u ag c Ser at t I Ie cca Pr o gag O31u at c I Ie gt g ValI g at As p cgc Ar g aag Ly s 135 gag O31u t ac Tyr tt c Ph e cgc Ar g tt c Ph e 40 tt g Le u ggc G31y cgc Ar g cca Pr o tt c Ph e 120 ct g Le u g aa O31u c ac Hi s cca Pr o cca Pr o c ag O31n tt c Ph e gt g ValI ag c Ser g aa O31u 1 05 t cc Ser gt g ValI ct c Le u cca Pr o cgc Ar g 10 cgc Ar g ct g Le u gca A a c ag O31n g aa O31u 90 g ac As p tt t Ph e g aa O31u c ag O31n gag O31u 1 70 c ag O31n gag O31u gt c ValI gcc A a ct c Le u ct c Le u aag Ly s ggt O31y Le u ct g Le u 1 gca A a c ag O31n c aa O31n ac c Thr aat As n ac c Thr aag Ly s ac c Thr t ac Tyr ct c Le u 140 c ac Hi s aag Ly s cgc Ar g gct A a at g vb t g aa O31u g aa O31u ct g Le u aag Ly s ggt O31y 125 gag O31u cca Pr a t ac Tyr ggc O31y gt c ValI ggt O31y t ac Tyr gca A a aac As n tt c Ph e 110 t ct Ser cca Pr a gag O31u tt t Ph e ag g Ar g aag Ly s aat As n cgc Ar g tt g Le u g ac As p tt c Ph e t gc cys ggc O31y c ag O31n aac As n 1 t aacacct tt gagagggaaa act <210O> 210 <21 1> 176 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anri cumn <400> 210 Ala O31u Ar g I'b t Ar g Ph e Se r Ph e Pr o Arg O31n O31n Ar g O31y Ar g Ph e 1 5 10 Leu Cys Ilie Ala Asp Phe I Ie Ar g Pro Ar g O31u O31n Ala ValI Lys Asp 25 Oy On Val Asp Val ~Ibt Pro Phe O1n Leu Val Thr ~Ibt 31y As n Pr o 40 li1e Ala Asp Phe Ala Asn O31u Leu Phe Ala Ala Asn Ou Tyr Ar g O31u 55 Page 264 Tyr O31u O31y Le u As p I Ie 145 Thr O31u Tr p ValI Tyr 115 O31u ValI Al a Hi s Ser As p G31y Ar g Le u ValI 1 65 G31y 70 Ar g Ph e Al a Al a Ser 150 Le u I Ie ValI As p Ar g Ly s 135 O31u Tyr seql i st car r ect ed. t xt O31y Val O31n Leu Thr O31u Ar g Ser O31u Leu Lys Leu 90 Pro O31u As p Lys Thr Lys 1 05 Phe Ser Phe O31y Tyr O31y 120 125 Leu Val 03 u Leu Leu 03 u 140 O31u Leu O31n Leu Hi s Pr o 1 Hi s Pr o O31u Al a Lys Ty r 1 70 Al a As n Ph e 110 Ser Pr o O31u Ph e Le u As p Ph e cys G31y O31n As n 1 <210O> 211 <21 1> 2599 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (2599) <223> RXN02198 <400> 211 agact agt gg cgct t t gcct gt gt t gct t a ggcggcgt t g agt t cgggaa t t gt ct aat c cgt act aagc t gt ct acaca aaaatgaact acgaatgaaa atg tct act tca gtt l'bt Ser Thr Ser Val act Thr tt g Le u c aa O31n t gt cys c ac Hi s tt t Ph e cca Pr o aac As n tt t Ph e gag O31u gcc Al a t gc Cys c ac Hi s gt g ValI ct g Le u ct c Le u tt t Ph e ct g Le u aac As n at c I Ie gt g ValI g ac As p gcg Al a aac As n gca Al a ggc O31y g aa O31u 45 ac c Thr gga O31y tt g Le u cat Hi s g ac As p aag Ly s cgc Ar g gct Al a gcg Al a t cc Ser gcc Al a tt c Ph e g at As p tt g Le u t at Tyr 1 g aa O31u at g vb t ct t Le u gt g ValI gt t ValI g ac As p tt t Ph e ggc O31y g at As p tt g Le u gag O31u at c I Ie tt g Le u ac c Thr ct g Le u ag g Ar g ac c Thr gct Al a g at As p c ag O31n gag O31u c ag O31n aat As n g at As p 1 00 t gc cgt gag ct t gcc t ac aag ggc act gca gt g gct agg gaa gt g gct Cys Arg Ou Leu Ala Tyr Lys Oy Thr Ala Val Ala Arg Ou Val Ala Page 265 seql i st cor r ect ed. t xt 105 110 115 gat gag atg ggg ccg ggc cga aac ggc atg cgg cgt ttc gtg gtt ggt 499 Asp Gu IVet y Pro y Arg Asn Gy IVet Arg Arg Phe Val Val y 120 125 130 tcc ctg gga cct gga acg aag ctt cca tcg ctg ggc cat gca ccg tat 547 Ser Leu G y Pro G y Thr Lys Leu Pro Ser Leu G y His Ala Pro Tyr 135 140 145 gca gat ttg cgt ggg cac tac aag gaa gca gcg ctt ggc atc atc gac 595 Ala Asp Leu Arg G y His Tyr Lys G u Ala Ala Leu G y lie lie Asp 150 155 160 165 ggt ggt ggc gat gcc ttt ttg att gag act gct cag gac ttg ctt cag 643 G y G y G y Asp Al a Phe Leu Ile G u Thr Al a G n Asp Leu Leu G n 170 175 180 gtc aag gct gcg gtt cac ggc gtt caa gat gcc atg gct gaa ctt gat 691 Val Lys Ala Ala Val His G y Val G n Asp Ala bet Ala G u Leu Asp 185 190 195 aca ttc ttg ccc att att tgc cac gtc acc gta gag acc acc ggc acc 739 Thr Phe Leu Pro lie II e Cys His Val Thr Val G u Thr Thr G y Thr 200 205 210 atg ctc atg ggt tct gag atc ggt gcc gcg ttg aca gcg ctg cag cca 787 IVet Leu IVet y Ser G u lle Gy Ala Ala Leu Thr Ala Leu G n Pro 215 220 225 ctg ggt atc gac atg att ggt ctg aac tgc gcc acc ggc cca gat gag 835 Leu y Ile Asp Iet I e y Leu Asn Cys Ala Thr y Pro Asp u 230 235 240 245 atg agc gag cac ctg cgt tac ctg tcc aag cac gcc gat att cct gtg 883 IVet Ser G u His Leu Arg Tyr Leu Ser Lys His Ala Asp lie Pro Val 250 255 260 tcg gtg atg cct aac gca ggt ctt cct gtc ctg ggt aaa aac ggt gca 931 Ser Val IVet Pro Asn Al a y Leu Pro Val Leu y Lys Asn y Ala 265 270 275 gaa tac cca ctt gag gct gag gat ttg gcg cag gcg ctg gct gga ttc 979 G u Tyr Pro Leu G u Al a u Asp Leu Ala G n Ala Leu Ala y Phe 280 285 290 gtc tcc gaa tat ggc ctg tcc atg gtg ggt ggt tgt tgt ggc acc aca 1027 Val Ser G u Tyr y Leu Ser bet Val y y Cys Cys y Thr Thr 295 300 305 cct gag cac atc cgt gcg gtc cgc gat gcg gtg gtt ggt gtt cca gag 1075 Pro G u His IIe Arg Al a Val Arg Asp Ala Val Val G y Val Pro G u 310 315 320 325 cag gaa acc tcc aca ctg acc aag atc cct gca ggc cct gtt gag cag 1123 Sn G u Thr Ser Thr Leu Thr Lys Ile Pro Ala y Pro Val G u G n 330 335 340 gcc tcc cgc gag gtg gag aaa gag gac tcc gtc gcg tcg ctg tac acc 1171 Ala Ser Arg G u Val G u Lys G u Asp Ser Val Ala Ser Leu Tyr Thr 345 350 355 tcg gtg cca ttg tcc cag gaa acc ggc att tcc atg atc ggt gag cgc 1219 Ser Val Pro Leu Ser n u Thr y Ile Ser IVet lie y u Arg 360 365 370 acc aac tcc aac ggt tcc aag gca ttc cgt gag gca atg ctg tct ggc 1267 Thr Asn Ser Asn G y Ser Lys Al a Phe Arg G u Al a Iet Leu Ser G y Page 266 seql i st cor r ect ed. t xt 375 380 385 gat tgg gaa aag tgt gtg gat att gcc aag cag caa acc cgc gat ggt 1315 Asp Trp G u Lys Cys Val Asp lie Ala Lys n G n Thr Arg Asp y 390 395 400 405 gca cac atg ctg gat ctt tgt gtg gat tac gtg gga cga gac ggc acc 1363 Al a Hi s IV t Leu Asp Leu Cys Val Asp Tyr Val G y Arg Asp G y Thr 410 415 420 gcc gat atg gcg acc ttg gca gca ctt ctt gct acc agc tcc act ttg 1411 Al a Asp IV t Al a Thr Leu Al a Al a Leu Leu Al a Thr Ser Ser Thr Leu 425 430 435 cca atc atg att gac tcc acc gag cca gag gtt att cgc aca ggc ctt 1459 Pro lie IV t lie Asp Ser Thr u Pro u Val Ile Arg Thr y Leu 440 445 450 gag cac ttg ggt gga cga agc atc gtt aac tcc gtc aac ttt gaa gac 1507 G u His Leu G y G y Arg Ser IIe Val Asn Ser Val Asn Phe G u Asp 455 460 465 ggc gat ggc cct gag tcc cgc tac cag cgc atc atg aaa ctg gta aag 1555 G y Asp G y Pro u Ser Arg Tyr G n Arg Ile IV t Lys Leu Val Lys 470 475 480 485 cag cac ggt gcg gcc gtg gtt gcg ctg acc att gat gag gaa ggc cag 1603 G n Hi s G y Ala Ala Val Val Ala Leu Thr IIe Asp G u G u G y n 490 495 500 gca cgt acc gct gag cac aag gtg cgc att gct aaa cga ctg att gac 1651 Ala Arg Thr Ala G u His Lys Val Arg Ile Ala Lys Arg Leu Ile Asp 505 510 515 gat atc acc ggc agc tac ggc ctg gat atc aaa gac atc gtt gtg gac 1699 Asp Ile Thr y Ser Tyr y Leu Asp Ile Lys Asp lie Val Val Asp 520 525 530 tgc ctg acc ttc ccg atc tct act ggc cag gaa gaa acc agg cga gat 1747 Cys Leu Thr Phe Pro Ile Ser Thr Gy n u u Thr Arg Arg Asp 535 540 545 ggc att gaa acc atc gaa gcc atc cgc gag ctg aag aag ctc tac cca 1795 Gy lie u Thr lie u Ala lie Arg u Leu Lys Lys Leu Tyr Pro 550 555 560 565 gaa atc cac acc acc ctg ggt ctg tcc aat att tcc ttc ggc ctg aac 1843 G u lie His Thr Thr Leu G y Leu Ser Asn IIe Ser Phe G y Leu Asn 570 575 580 cct gct gca cgc cag gtt ctt aac tct gtg ttc ctc aat gag tgc att 1891 Pro Ala Ala Arg G n Val Leu Asn Ser Val Phe Leu Asn G u Cys Ile 585 590 595 gag gct ggt ctg gac tct gcg att gcg cac agc tcc aag att ttg ccg 1939 G u Ala G y Leu Asp Ser Ala lie Ala His Ser Ser Lys IIe Leu Pro 600 605 610 atg aac cgc att gat gat cgc cag cgc gaa gtg gcg ttg gat atg gtc 1987 IV t Asn Arg Ile AspAsp Ar g n Ar g u Val A a Leu Asp IVt Val 615 620 625 tat gat cgc cgc acc gag gat tac gat ccg ctg cag gaa ttc atg cag 2035 Tyr Asp Arg Arg Thr G u Asp Tyr Asp Pro Leu n G u Phe IV t G n 630 635 640 645 ctg ttt gag ggc gtt tct gct gcc gat gcc aag gat gct cgc gct gaa 2083 Leu Phe G u y Val Ser Ala Ala Asp Ala Lys Asp Ala Arg Ala G u Page 267 seqI i st car r ect ed. t xt c ag O31n g ac As p gag O31u aag Ly s 710 gt g ValI ccg Pr o G31y at c I Ie gt g ValI 790 gcg Al a aag Ly s gcc Al a g at As p 680 t ct Ser gt g ValI c aa O31n at g O31y 760 aag Ly s aac As n g aa O31u ac c Thr 650 at g vb t aat As n at t I Ie gag O31u gca Al a 730 gag O31u at c I Ie tt g Le u 99c O31y aaa Ly s 810 gt g ValI cct Pr o O31y gcg Al a ct g Le u 71 5 gaa O31u gaa O31u gt c ValI gt g ValI at c I Ie 795 gca Al a at g vb t tt g Le u ct t Le u at c I Ie 700 tt t Ph e ac c Thr gca Al a gt g ValI g ac As p 780 aag Ly s g ac As p aag Ly s tt t Ph e gag O31u 685 at c I Ie ggt O31y at g vb t g aa O31u gcc Al a 765 at c I Ie c ag O31n gt c ValI c aa O31n gag O31u 670 g at As p aac As n t cc Ser aaa Ly s gct Al a 750 ac c Thr at t I Ie cca Pr o at c I Ie ac c Thr 830 655 cgt tt g Arg Leu gat ct g Asp Leu gag gac O31u As p gga cag O31y O31n 720 act gcg Thr Al a 735 acc gga t Thr 03 y gt c aag Val Lys t tg t cc Leu Ser ct g t cc Leu Ser 800 ggc at g t 31y Ib t 815 at c agc Il e Ser Hi s Asn 10 Val Leu Leu Asp Leu Asn Phe 03 u 75 Page 268 gca Al a j aa ct t e u 705 at g gt g ValI [ct Se r ggt aac As n 785 gcc Al a ~cg Se r j ac As p c ag O31n gca Al a 690 ct c Le u c ag O31n gcc Al a gcg Al a g ac As p 770 aac As n at g vb t gga O31y 660 at c I Ie at g O31y cca Pr o Le u 740 gca Al a c ac Hi s t ac Tyr g aa O31u ct t Le u 820 at c I Ie aag Ly s at g vb t tt c Ph e 725 g aa O31u gag O31u g at As p g ac As p gca Al a 805 gt g ValI 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2599 <210O> 212 <21 1> 833 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 212 I'bt Ser Thr Ser Val Thr Ser Pro Al a 1 5 O3 u Phe Leu Asp Al a Leu Al aAsn Hi s 25 I'b t O31y Th r O31n Leu O31n (31y Phe Asp 40 Leu Asp Leu 03 u 03 y Cy s As n 03 u I I e 55 Val Leu Arg O31n Ilie His Arg Ala Tyr 70 Asn Al a Hi s Ser Ser 1I1 e 03 y Asp 03 y Al a Val 03 u Lys Asp Phe As p Thr Arg Pro Asp Al a 03 y Al a Asp Leu seql i st cor r ect ed. t xt Val G u Thr Asn Thr Phe y Cys Asn Leu Pro Asn Leu 90 Al a Asp Tyr Asp Al a Ar g Sy 145 Leu G n Met G u Thr 225 Thr Al a Sy Al a Cys 305 Val Sy Al a bet Al a 385 G n Sy Thr Al a G u 115 Val Al a SIle Leu G u 195 Thr Leu Pr o l Ie Asn 275 Al a Sy Val Val Leu 355 Sy Leu Ar g Asp Ser 435 Ar g Al a Sy Tyr Asp 165 G n Asp Thr Pr o G u 245 Val Al a Phe Thr G u 325 G n Thr Ar g Sy Sy 405 Thr Leu Cys Asp Ser Al a 150 Sy Val Thr Met Leu 230 Met Ser G u Val Pr o 310 G n Al a Ser Thr Asp 390 Al a Al a Pr o Ar g G u Leu 135 Asp Sy Lys Phe Leu 215 Sy Ser Val Tyr Ser 295 G u G u Ser Val Asn 375 Tr p Hi s Asp SI e Leu 105 Sy Pr o Ar g Asp Al a 185 Pr o Sy Asp Hi s Pr o 265 Leu Tyr SIle Ser G u 345 Leu Asn Lys Leu Al a 425 I e Tyr Sy Thr Hi s 155 Phe Hi s SIle Su I e 235 Ar g Al a Al a Leu Al a 315 Leu G u G n Ser Val 395 Leu Leu Lys Ar g Lys 140 Tyr Leu Sy Cys SIle 220 Sy Tyr Sy G u Ser 300 Val Thr Lys G u Lys 380 Asp Cys Al a Sy Asn 125 Leu Lys SIle Val Hi s 205 Sy Leu Leu Leu Asp 285 let Ar g Lys G u Thr 365 Al a SIle Val Al a Thr 110 Sy Pr o G u G u G n 190 Val Al a Asn Ser Pr o 270 Leu Val Asp SIle Asp 350 Sy Phe Al a Asp Leu 430 Al a bet Ser Al a Thr 175 Asp Thr Al a Cys Lys 255 Val Al a Sy Al a Pr o 335 Ser SIle Ar g Lys Tyr 415 Leu Val Ar g Leu Al a 160 Al a Al a Val Leu Al a 240 Hi s Leu G n Sy Val 320 Al a Val Ser G u G n 400 Val Al a Asp Ser Thr G u Pro G u Val 445 Page 269 seqI i st car r ect ed. t xt I Ie Ar g 450 Val As n 465 I'b t Lys As p (1u Lys Ar g As p II e 530 G3 u Thr 545 Lys Lys Ser Phe Leu Asn Ser Lys 610 Al a Leu 625 O31n (1u As p Al a Al a G3 n a3 u Al a 690 Leu Leu 705 [Vb t (1n Val Al a Ser Al a 31y As p 770 As n As n 785 Thr Gy Leu Ou His Leu Gy Gy Arg Ser I Ie Val Asn Ser 455 Ph e Le u O31u Le u 515 ValI Ar g Le u G y O31u 595 li1e As p Ph e Ar g Ar g 675 G31y As n Le u Tyr O31n 755 ValI O31y As p Lys5 485 O31n As p As p As p Pr o 565 As n I Ie Pr o ValI O31n 645 O31u I Ie Lys5 vb t Ph e 725 O31u O31u As p As p As p Hi s Ar g I Ie Le u 535 li1e I Ie Al a Al a As n 615 As p Ph e Le u G31y Lys5 695 Thr Le u Ph e Lys5 G31y 775 ValI G31y G31y Thr Thr 520 Thr O31u Hi s Al a G31y 600 Ar g Ar g O31u Al a As p 680 Ser ValI O31n vb t O31y 760 Lys5 As n Pr o Al a Al a 505 O31y Ph e Thr Thr Ar g 585 Le u I Ie Ar g O31y Al a 665 Ly s Pr o O31y Ser O31u 745 Ly s As n Le u O31u Al a 490 O31u Ser Pr a I Ie Thr 570 G n As p As p Thr ValI 650 vb t As n I Ie O31u Al a 730 O31u I Ie Le u O31y Ser 475 ValI Hi s Tyr I Ie O31u 555 Le u ValI Ser As p O31u 635 Ser Pr a O31y Al a Le u 715 O31u O31u ValI ValI I Ie 795 460 Ar g ValI Ly s O31y Ser 540 Al a O31y Le u Al a Ar g 620 As p Al a Le u Le u I Ie 700 Ph e Thr Al a ValI As p 780 Ly s Tyr Al a ValI Le u 525 Thr I Ie Le u As n I Ie 605 O31n Tyr Al a Ph e O31u 685 li1e O31y vb t O31u Al a 765 li1e O31n Ar g Thr 495 li1e I Ie O31n O31u As n 575 ValI Hi s O31u Pr a Al a 655 Ar g As p O31u O31y Thr 735 Thr ValI Le u Le u I Ie 480 li1e Al a Ly s O31u Le u 560 li1e Ph e Ser ValI Le u 640 Ly s Le u Le u As p O31n 720 Al a O31y Ly s Se r Se r 800 Aa N'bt Leu Ou AaAia OuOu His Lys AiaAsp Val Ile Oy Ibt Page 270 seqi i st car r ect ed. t xt 805 810 815 Ser G3 y Leu Leu Val Lys Ser Thr Val Val l'bt Lys (3 n Thr I I e Ser 820 825 830 Asp <210O> 213 <21 1> 2578 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2578) <223> FRXA02198 <400> 213 agact agt gg cgct t t gcct gt gt t gct t a ggcggcgt t g agt t cgggaa t t gt ct aat c cgt act aagc t gt ct acaca aaaatgaact acgaatgaaa at g t ct l'bt Ser act t ca gt t Thr Ser Val act Thr tt g Le u c aa O31n t gt CYS c ac Hi s tt t Ph e t gc CYS g at As p t cc Ser gca A a 1 50 ggt G31y cca Pr o aac As n tt t Ph e gag (31u gcc A a t gc cys gag (31u at g vb t 120 gga G31y tt g Le u ggc G31y c ac Hi s gt g ValI ct g Le u ct c Le u tt t Ph e ct g Le u gcc A a ccg Pr o gga G31y G31y gcc A a aac As n at c I Ie gt g ValI g ac As p gcg A a aac As n aag Ly s cga Ar g aag Ly s 140 t ac Tyr tt g Le u gca A a ggc G31y g aa (31u 45 ac c Thr gga G31y Le u ggc G31y aac As n 125 ct t Le u aag Ly s at t I Ie cat t cc Hi s Ser 15 gac ggc As p (1y aag gat Lys As p cgc cct Ar g Pr o gct gac A a As p gcg gat A a As p 95 act gca Thr A a 110 ggc at g 31y lb t cca t cg Pr o Ser gaa gca (3 u A a gag act 03 u Thr t cc Ser gcc A a tt c Ph e g at As p tt g Le u t at Tyr gt g ValI cgg Ar g ct g Le u gcg A a 1 1 g aa (31u at g vb t ct t Le u gt g ValI gt t ValI g ac As p gct A a cgt Ar g ggc G31y 145 ct t Le u tt t Ph e ggc G31y g at As p tt g Le u gag (31u at c I Ie ag g Ar g tt c Ph e 130 cat Hi s ggc G31y tt g Le u ac c Thr ct g Le u ag g Ar g ac c Thr gct A a g aa (31u 115 gt g ValI gca A a at c I Ie g at As p c ag O31n gag O31u c ag O31n aat As n g at As p 1 00 gt g ValI gt t ValI ccg Pr o at c I Ie gct cag gac t tg ct t cag A a 03 n Asp Leu Leu 03 n Page 271 seql i st cor r ect ed. t xt gt c Val aca Thr at g Met ct g Leu 230 at g Met tcg Ser gaa G u gt c Val cct Pr o 310 cag G n gcc Al a tcg Ser acc Thr gat Asp 390 gca Al a gcc Al a cca Pr o gct Al a ttg Leu 200 at g Met at c SIle gag G u at g Met cca Pr o 280 gaa G u cac Hi s acc Thr cgc Ar g cca Pr o 360 t cc Ser gaa G u at g Met at g Met at g Met gtt Val at t SIle tct Ser at g Met ct g Leu 250 aac Asn gag G u ggc Gy cgt Ar g aca Thr 330 gt g Val t cc Ser ggt Gy tgt Cys gat Asp 410 acc Thr gac Asp cac Hi s at t SIle gag G u at t SI e 235 cgt Ar g gca Al a gct Al a ct g Leu gcg Al a 315 ct g Leu gag G u cag G n t cc Ser gt g Val 395 ctt Leu tt g Leu t cc Ser ggc Gy tgc Cys at c SIle 220 ggt Gy t ac Tyr ggt Gy gag G u t cc Ser 300 gt c Val acc Thr aaa Lys gaa G u aag Lys 380 gat Asp tgt Cys gca Al a acc Thr gtt Val cac Hi s 205 ggt Gy ct g Leu ct g Leu ctt Leu gat Asp 285 at g bet cgc Ar g aag Lys gag G u acc Thr 365 gca Al a at t SIle gt g Val gca Al a gag G u 175 caa gat gcc G n Asp Al a 190 gt c acc gt a Val Thr Val gcc gcg ttg Al a Al a Leu aac tgc gcc Asn Cys Al a 240 t cc aag cac Ser Lys Hi s 255 cct gtc ct g Pro Val Leu 270 ttg gcg cag Leu Al a G n gtg ggt ggt Val Gy y gat gcg gt g Asp Al a Val 320 at c cct gca I e Pro Al a 335 gac t cc gt c Asp Ser Val 350 ggc att tcc G y Ile Ser ttc cgt gag Phe Arg G u gcc aag cag Al a Lys G n 400 gat tac gtg Asp Tyr Val 415 ctt ctt gct Leu Leu Al a 430 cca gag gtt Pro u Val at g Met gag G u aca Thr 225 acc Thr gcc Al a ggt Gy gcg Al a tgt Cys 305 gtt Val ggc Gy gcg Al a at g Met gca Al a 385 caa G n gga Gy acc Thr gct Al a acc Thr 210 gcg Al a ggc Gy gat Asp aaa Lys ct g Leu 290 tgt Cys ggt Gy cct Pr o tcg Ser at c SI e 370 at g Met acc Thr cga Ar g agc Ser gaa G u 195 acc Thr ct g Leu cca Pr o at t SIle aac Asn 275 gct Al a ggc Gy gtt Val gtt Val ct g Leu 355 ggt Gy ct g Leu cgc Ar g gac Asp t cc Ser 435 ctt Leu ggc Gy cag G n gat Asp cct Pr o 260 ggt Gy gga Gy acc Thr cca Pr o gag G u 340 t ac Tyr gag G u t ct Ser gat Asp ggc Gy 420 act Thr gat Asp acc Thr cca Pr o gag G u 245 gt g Val gca Al a ttc Phe aca Thr gag G u 325 cag G n acc Thr cgc Ar g ggc Gy ggt Gy 405 acc Thr ttg Leu 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 att cgc aca ggc ctt lie Arg Thr y Leu Page 272 seql i st cor r ect ed. t xt 440 445 450 gag cac ttg ggt gga cga agc atc gtt aac tcc gtc aac ttt gaa gac 1507 G u His Leu G y G y Arg Ser lie Val Asn Ser Val Asn Phe G u Asp 455 460 465 ggc gat ggc cct gag tcc cgc tac cag cgc atc atg aaa ctg gta aag 1555 Sy Asp y Pro u Ser Arg Tyr G n Arg lie IVet Lys Leu Val Lys 470 475 480 485 cag cac ggt gcg gcc gtg gtt gcg ctg acc att gat gag gaa ggc cag 1603 G n Hi s y Ala Ala Val Val Ala Leu Thr Ile Asp G u G u G y G n 490 495 500 gca cgt acc gct gag cac aag gtg cgc att gct aaa cga ctg att gac 1651 Ala Arg Thr Ala G u His Lys Val Arg Ile Ala Lys Arg Leu Ile Asp 505 510 515 gat atc acc ggc agc tac ggc ctg gat atc aaa gac atc gtt gtg gac 1699 Asp Ile Thr y Ser Tyr y Leu Asp Ile Lys Asp lie Val Val Asp 520 525 530 tgc ctg acc ttc ccg atc tct act ggc cag gaa gaa acc agg cga gat 1747 Cys Leu Thr Phe Pro Ile Ser Thr y n G u G u Thr Arg Arg Asp 535 540 545 ggc att gaa acc atc gaa gcc atc cgc gag ctg aag aag ctc tac cca 1795 Sy lie u Thr lie u Ala lie Arg u Leu Lys Lys Leu Tyr Pro 550 555 560 565 gaa atc cac acc acc ctg ggt ctg tcc aat att tcc ttc ggc ctg aac 1843 G u lie His Thr Thr Leu G y Leu Ser Asn IIe Ser Phe G y Leu Asn 570 575 580 cct gct gca cgc cag gtt ctt aac tct gtg ttc ctc aat gag tgc att 1891 Pro Ala Ala Arg G n Val Leu Asn Ser Val Phe Leu Asn G u Cys Ile 585 590 595 gag gct ggt ctg gac tct gcg att gcg cac agc tcc aag att ttg ccg 1939 G u Ala G y Leu Asp Ser Ala IIe Ala His Ser Ser Lys IIe Leu Pro 600 605 610 atg aac cgc att gat gat cgc cag cgc gaa gtg gcg ttg gat atg gtc 1987 IVet Asn Arg ile Asp Asp Ar g n Ar g u Val Ala Leu Asp Iet Val 615 620 625 tat gat cgc cgc acc gag gat tac gat ccg ctg cag gaa ttc atg cag 2035 Tyr Asp Arg Arg Thr G u Asp Tyr Asp Pro Leu n G u Phe IVet G n 630 635 640 645 ctg ttt gag ggc gtt t ct gct gcc gat gcc aag gat gct cgc gct gaa 2083 Leu Phe G u y Val Ser Ala Ala Asp Ala Lys Asp Ala Arg Ala G u 650 655 660 cag ctg gcc gct atg cct ttg ttt gag cgt ttg gca cag cgc atc atc 2131 G n Leu Ala Ala IVet Pro Leu Phe G u Arg Leu Ala G n Arg lie lie 665 670 675 gac ggc gat aag aat ggc ctt gag gat gat ctg gaa gca ggc atg aag 2179 Asp y Asp Lys Asn G y Leu G u Asp Asp Leu G u Al a y IVet Lys 680 685 690 gag aag tct cct att gcg atc atc aac gag gac ctt ctc aac ggc atg 2227 Su Lys Ser Pro lie Ala lie lie Asn u Asp Leu Leu Asn y IVet 695 700 705 aag acc gtg ggt gag ctg ttt ggt tcc gga cag atg cag ctg cca ttc 2275 Lys Thr Val y G u Leu Phe y Ser y G n Iet G n Leu Pro Phe Page 273 seql i st cor r ect ed. t xt 710 715 720 725 gtg ctg caa tcg gca gaa acc atg aaa act gcg gtg gcc tat ttg gaa 2323 Val Leu G n Ser Ala G u Thr Met Lys Thr Ala Val Ala Tyr Leu G u 730 735 740 ccg ttc atg gaa gag gaa gca gaa gct acc gga tct gcg cag gca gag 2371 Pro Phe Met u u u Ala u Ala Thr y Ser Ala n Ala u 745 750 755 ggc aag ggc aaa atc gtc gtg gcc acc gtc aag ggt gac gtg cac gat 2419 Sy Lys y Lys lie Val Val Ala Thr Val Lys y Asp Val His Asp 760 765 770 atc ggc aag aac ttg gtg gac atc att ttg tcc aac aac ggt tac gac 2467 Ile y Lys Asn Leu Val Asp lie lie Leu Ser Asn Asn y Tyr Asp 775 780 785 gtg gtg aac ttg ggc atc aag cag cca ctg tcc gcc atg ttg gaa gca 2515 Val Val Asn Leu G y Ile Lys G n Pro Leu Ser Al a IVet Leu G u Al a 790 795 800 805 gcg gaa gaa cac aaa gca gac gtc atc ggc atg tcg gga ctt ctt gtg 2563 Ala u u His Lys Ala Asp Val Ile Gy IVet Ser y Leu Leu Val 810 815 820 aag tcc acc gtg gtg 2578 Lys Ser Thr Val Val 825 <210> 214 <211> 826 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 214 IVet Ser Thr Ser Val Thr Ser Pro Ala His Asn Asn Ala His Ser Ser 1 5 10 Su Phe Leu Asp Ala Leu Ala Asn His Val Leu I e Gy Asp Gy Ala 25 IVet G y Thr G n Leu n G y Phe Asp Leu Asp Val G u Lys Asp Phe 40 Leu Asp Leu G u G y Cys Asn G u Ile Leu Asn Asp Thr Arg Pro Asp 55 Val Leu Arg G n lie His Arg Ala Tyr Phe G u Ala G y Ala Asp Leu 70 75 Val G u Thr Asn Thr Phe y Cys Asn Leu Pro Asn Leu Ala Asp Tyr 90 Asp lie Ala Asp Arg Cys Arg G u Leu Ala Tyr Lys y Thr Ala Val 100 105 110 Ala Arg u Val Ala Asp u ibet y Pro y Arg Asn y ibet Arg 115 120 125 Arg Phe Val Val y Ser Leu y Pro y Thr Lys Leu Pro Ser Leu 130 135 140 G y His Ala Pro Tyr Al a Asp Leu Arg G y His Tyr Lys G u Ala Ala 145 150 155 160 Leu G y Ile Ile Asp G y G y G y Asp Al a Phe Leu Ile G u Thr Al a Page 274 seqi i st car r ect ed. t xt 1 65 O31n Val Lys Ala O31n O31u Thr 225 Thr A a O31y A a CYS 305 ValI O31y A a vb t A a 385 O31n O31y Thr I Ie ValI 465 vb t As p Ly s Le u (31u 1 95 Thr Le u Pr o I Ie As n 275 A a G31y ValI ValI Le u 355 G31y Le u Ar g As p Ser 435 Thr Ph e Le u O31u Le u 515 As p Thr Pr o O31u 245 ValI A a Ph e Thr O31u 325 O31n Thr Ar g O31y O31y 405 Thr Le u Le u As p Ly s 485 O31n As p Thr vb t Le u 230 vb t Ser O31u ValI Pr o 310 O31n A a Ser Thr As p 390 A a A a Pr o O31u O31y 470 O31n A a As p Ph e Le u 215 O31y Ser ValI Tyr Ser 295 O31u O31u Ser ValI As n 375 Tr p Hi s As p I Ie Hi s 455 As p Hi s Ar g I Ie 1 A a Val 1 85 Pr o I Ie O3 y Ser As p l'b t Hi s Leu 250 Pr o As n 265 Leu 03 u Ty r 03 y I Ie Ar g Ser Thr 330 O3 u Val 345 Leu Ser As n (1y Lys Cys Leu Asp 410 A a Thr 425 1 1 e As p O31y (1y Pro O31u A a A a 490 A a 03 u 505 O3 y Ser Hi s 03 y Val I Ie Ou le 235 Ar g A a A a Le u A a 315 Le u O31u O31n Ser ValI 395 Le u Le u Ser Ar g Ser 475 ValI Hi s Cys I Ie 220 O31y Tyr O31y O31u Ser 300 ValI Thr Ly s O31u Ly s 380 As p Cys A a Thr Ser 460 Ar g ValI Ly s Hi s 205 O31y Le u Le u Le u As p 285 vb t Ar g Ly s O31u Thr 365 A a I Ie ValI A a O31u 445 li1e Tyr A a ValI O31n 1 ValI A a As n Ser Pr a 270 Le u ValI As p I Ie As p 350 O31y Ph e A a As p Le u 430 Pr a ValI O31n Le u Ar g 510 As p Thr A a Cys Ly s 255 ValI A a O31y A a Pr a 335 Ser I Ie Ar g Ly s Tyr 415 Le u O31u As n Ar g Thr 495 li1e A a ValI Le u A a 240 Hi s Le u O31n O31y ValI 320 A a ValI Ser O31u O31n 400 ValI A a ValI Ser I Ie 480 li1e A a Tyr 03 y Leu Asp I I e Lys 525 Page 275 seqi i st car r ect ed. t xt Asp I Ie Val Val Asp Cys Leu Thr Phe Pro I Ie Ser Thr 530 535 540 (31y (1n (1u O31u 545 Ly s Ser Le u Ser A a 625 O31n As p A a O31u Le u 705 vb t ValI Ser G31y As n 785 A a Ser Ar g Le u G31y O31u 595 li1e As p Ph e Ar g Ar g 675 G31y As n Le u Ty r O31n 755 ValI G31y Le u Le u As p Pr o 565 As n I Ie Pr o ValI O31n 645 O31u I Ie Ly s vb t Ph e 725 O31u O31u As p As p A a 805 le le A a A a As n 615 As p Ph e Le u G31y Ly s 695 Th r Le u Ph e Ly s O31y 775 ValI aOu O31u Hi s A a G31y 600 Ar g Ar g O31u A a As p 680 Ser ValI O31n vb t O31y 760 Ly s As n aOu Th r Th r Ar g 585 Le u I Ie Ar g O31y A a 665 Ly s Pr o O31y Ser O31u 745 Ly s As n Le u Hi s I Ie Th r 570 O31n As p As p Th r ValI 650 vb t As n I Ie O31u A a 730 O31u I Ie Le u G31y Ly s 810 O31u 555 Le u ValI Ser As p O31u 635 Ser Pr o G31y A a Le u 715 O31u O31u ValI ValI I Ie 795 A a I Ie Le u As n I Ie 605 O31n Ty r A a Ph e O31u 685 li1e O31y vb t O31u A a 765 li1e O31n ValI O31u As n 575 ValI Hi s O31u Pr a A a 655 Ar g As p O31u O31y Th r 735 Th r ValI Le u Le u O31y 815 Le u 560 li1e Ph e Ser ValI Le u 640 Ly s Le u Le u As p O31n 720 A a O31y Ly s Ser Ser 800 b t Leu Val Lys Ser Thr Val Val <210O> <211 <212> <213> <220> <221 <222> <223> 215 621 DNA Cor ynebact er i urn gi ut ani cumn ODS 101). (598) RXN03 074 Page 276 seqI i st car r ect ed. t xt <400> 215 t t tgt gggca at ctggt t tt t tcgt aat tg tgt gggat gaatct ct taaa aat tcacat t t agcaggaca agcat act gt t t t agt t ct a t gct gt gggc at g act caa agt gct I'bt Thr 03 n Ser Al a cca Pr o gcg Al a tt c Ph e ct g Le u g at As p gga G31y at t I Ie ct t Le u g ac As p gt c ValI 1 50 c ag O31n tt c Ph e t ca Ser gga G31y t cc Ser g ac As p gga G31y g ac As p ac c Thr 120 gt g ValI gcg Al a gcc Al a g ac As p at a I Ie ct g Le u t cc Ser g at As p gca Al a ccg Pr o t cg Ser t ct Ser gca Al a c ag O31n ac c Thr gag O31u c ac Hi s ggt G31y at c I Ie aaa Ly s ggt G31y 140 gga G31y ct c Le u c aa O31n aaa Ly s aat As n gcg Al a t cc Ser ac c Thr 110 act Thr at t I Ie gt a ValI 15 aac As n t gc cys cct Pr o ct a Le u gga G31y 95 at g vb t aaa Ly s g ac As p g ac As p ct t Le u tt c Ph e ggg G31y gt t ValI 80 gt a ValI ac c Thr act Thr tt c Ph e 1 at c I Ie gga G31y c aa O31n gga O31y 65 ggc O31y at t I Ie tt t Ph e ggt O31y at t I Ie 145 at c I Ie ggt O31y g ac As p 50 gt g ValI g ac As p gt c ValI ggt O31y t cc Ser 130 cct Pr o g ac As p 20 ac a Thr gcc Al a gt t ValI at t I Ie gga O31y 1 00 aaa Ly s g aa O31u cat Hi s aac As n g aa O31u ct c Le u at c I Ie gca Al a gca Al a gcc Al a cga Ar g t ac Tyr aag Ly s 1 at c gt c acc gag gcg cca at t lieVal Thr Ou Ala Pro Ilie 1 60 t aat t tgt tt t gacgacgca gt a <210O> 216 <211> 166 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 216 I'bt Thr O31n Ser Al a Pro O31u Phe I Ie Al a Thr Al a Asp Leu Val Asp 1 5 10 Il e Ile 0 y Asp Asn Al a 03 n Se r Cys As p Thr 03 n Phe 03 n Asn Leu 25 O31y O31y Al a Th r O31u Ph e Hi s O31y IIe I Ie Thr Thr Val Lys Cys Phe 40 O31n Asp As n Al a Leu Leu Lys Ser Ilie Leu Ser Ou Asp Asn Pro Oy Page 277 Gy Gy SIle Phe Gy SIle 145 G u Leu I le Asn Cys 115 Gy Gy Pr o I le Al a Al a Al a Ar g Tyr Lys 165 Gy Leu Ar g Gy Val 135 Tyr seql i st cor r ect ed. t xt Asp Ala Ser Val His Thr Gy Lys Asp His Gy Trp 90 Asp Ser Ala Val I e y 105 Thr Asn Pro Arg Lys Ser 120 125 Val Val Ser I e G y y 140 Al a Asp Ser Asp G y I e 155 Al a Ser Thr 110 Thr SIle SIle Leu Gy Met Lys Asp Val <210> 217 <211> 621 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(598) <223> FRXA02906 <400> 217 tttgtgggca atctggtttt ttcgtaattg tgtgggatga tagcaggaca agcatactgt tttagt t cta tgctgtgggc atctct t aaa aat t cacatt cca Pr o gcg Al a ttc Phe ct g Leu gat Asp gga G y at t SIle ttc Phe t ca Ser gga G y t cc Ser gac Asp gga G y gac Asp gcc Al a gac Asp at a SIle ct g Leu t cc Ser gat Asp gca Al a gca Al a cag G n acc Thr gag G u cac Hi s ggt G y at c l Ie ct c Leu caa G n aaa Lys aat Asn gcg Al a t cc Ser acc Thr 110 gt a Val 15 aac Asn tgc Cys cct Pr o ct a Leu gga G y 95 at g M\et gac Asp ctt Leu ttc Phe ggg G y gtt Val 80 gt a Val acc Thr at g let 1 at c SIle gga G y caa G n gga G y ggc G y at t SIle ttt Phe act Thr at c SIle ggt G y gac Asp gt g Val gac Asp gt c Val ggt Gy caa G n ggc Gy gcc Al a aac Asn ct g Leu at c SIle aac Asn tgt Cys 115 agt Ser gac Asp aca Thr gcc Al a gtt Val at t SIle gga G y 100 aaa Lys gct Al a aac Asn gaa G u ct c Leu at c SIe gca Al a gca Al a gcc Al a Page 278 seqI i st car r ect ed. t xt ctt gga acc aac ccg cgg aaa tcc act aaa act ggt tcc ggc gaa cga Leu G31y Thr Asn Pro Arg Lys Ser Thr Lys Thr G31y Ser G31y O31u Ar g 120 125 130 gacgt agt ggt a tcg att ggt ggc at t gact t cat t cct ggt cat t ac Asp Val Val Val Ser Ilie 31y 31y Ilie Asp Phe Ilie Pr o G31y Hi s Tyr 135 140 145 gt c tacgcg gac tct gac gga at t at cgt cacc gag gcg cca at t aag Val Tyr Ala Asp Ser Asp 31y Ilie Ilie Val Thr O31u Ala Pr o Ilie Lys 150 155 160 165 cag t aat t tgt tt t gacgacgca gt a O31n <210> 218 <211> 166 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 218 vb t le O31y O31n O31y O31y I Ie Ph e O31y I Ie 145 O31u Thr 03 n Se r I I e 03 y As r 2 C 03 y A a Th r As p As n AE Val Leu Val As p II e I IE Val As nO(1 1 OC O3 y Cys LyE 115 Ser (3 y (30 130 Pro O31y Hi E Ala Pr o I IE A a As n O31u Le u I Ie A a A a A a Ar g Tyr Lys5 1 Pro Ou Phe Ilie A a Ph e Le u As p 70 O31y I Ie Le u As p ValI 150 O31n O31n Hi s Lys5 55 O31y Le u Ar g O31y ValI 135 Tyr Cys 25 li1e I Ie A a Lys5 Ser 1 05 As n ValI As p A a 10 As p I Ie Le u Ser As p 90 A a Pr o Ser Ser Thr Thr Thr Ser ValI Hi s ValI Ar g I Ie As p 1 A a O31n Thr O31u Hi s O31y I Ie Lys5 O31y 140 O31y As p Ph e ValI As p Thr Tr p O31y Ser 125 Oy le Le u O31n Lys5 As n A a Ser Thr 110 Thr I Ie I Ie ValI As n Cys Pr o Le u O31y vb t As p ValI <210O> <211 <21 2> <21 3> <220> <221 <222> <223> 219 1 557 DNA Cor ynebact er i urn gi ut ani cumn CIDS 101). (1534) RXN001 32 Page 279 seqi i st car r ect ed. t xt <400> 219 aacagct t ca at caat t cgg t gt ccact cc aacat gt aga gt ggt gcgcg t t aaaaaagt tttcctaatt ttcattttct taaaaggagc tcgccaggac atg gca cag gtt atg l'bt Ala On Val l'b t gac Asp at t l Ie gaa O31u at c l Ie ttg Leu gat Asp cca Pr o t ac Tyr aac Asn ggt 3y 150 gat Asp gct Al a gt c Val gaa O31u acc Thr 230 aag Lys ct t Leu gca Al a at g lvb t gct Al a gct Al a ggt 3y t gg Tr p 120 at c l Ie gaa O31u gat Asp gag O31u gag O31u 200 ggc G31y t cc Ser gcc Al a gag O31u gag O31u gt c Val gt t Val gcg Al a cca Pr o at c l Ie gac Asp gag O31u t ac Tyr 170 ggc G31y acc Thr ct g Leu ttt Phe gat Asp t at Tyr cag O31n cag O31n cgt Ar g 75 gct Al a gt a Val aac Asn gac Asp cag O31n 155 at c l Ie aag Lys acc Thr cct Pr o gat Asp 235 ct t Leu gag O31u cct Pr o acc Thr t gg Tr p at c l Ie ttc Phe cag O31n ggc O31y 140 gct Al a gca Al a t gg Tr p acc Thr ttc Phe 220 aac Asn t ca Ser at g lvb t ttg Leu 45 gcc Al a gct Al a gt t Val gcg Al a at c l Ie 125 ggt O31y ggt O31y ttc Phe ggc O31y ggt O31y 205 cca Pr a aag Lys ct a Leu cca Pr a aag Lys gt g Val t cc Ser gt c Val t gg Tr p 110 ttc Phe gac Asp ct g Leu ttg Leu aag Lys 190 gt g Val gcg Al a t ac Tyr gca Al a 15 ggt O31y ggc O31y ct t Leu t gc Cys ggc O31y 95 aag Lys agc Ser gcc Al a gt t Val ggc O31y 175 at c l Ie cac Hi s at g lvb t ggc gag O31u ct C Leu gcc Al a at t l Ie aac Asn 80 t cc Ser ggt O31y t gg Tr p acc Thr cca Pr a 160 at g lvb t gct Al a cgc Ar g aac Asn acc Thr 240 1 gca Al a at g lvb t cga Ar g gag O31u at t l Ie ggc 99C O31y gag O31 u ggc O31y at g 145 cca Pr a ct g Leu gag O31u ct g Leu gt c Val 225 cgc Ar g gga O31y cag O31n at t I e acc Thr ttc Phe acc Thr t ca Ser gat Asp 130 gct Al a gca Al a cgt Ar g gcc Al a t ac Tyr 210 aac Asn cac Hi s cac Hi s 20 cgc Ar g ggt O31y act Thr acc Thr g aa O31u 100 gag O31u ct g Leu at t l Ie gcc Al a gt t Val 180 aag Lys ttc Phe gct Al a ct g Leu cag O31n aag Lys t ct Ser gct Al a cag O31n gag O31u gag O31u cca Pr a cgc Ar g aac Asn 165 ct t Leu ggt O31y gct Al a gt c Val at c l Ie 245 gac ggc atc aac cgc gcc act gac atg ctc atg ggc ggc aag aac gtg Page 280 Asp Gy IlieAsn Arg 250 seqI i st car r ect ed. t xt Al a Thr Asp I'bt Leu I'bt (31y (31y ct t Le u g ac As p gct Al a gcc Al a 310 at c G31y c ac Hi s tt c Ph e ct g Le u 390 aac As n g aa O31u g aa O31u g aa (31u t gc cys c ag O31n 280 c ag O31n gag O31u t cc Ser at c I Ie g ac As p 360 tt c Ph e aac As n tt c Ph e c ag O31n gt g ValI 440 ac c Thr t ac Tyr gct Al a ct g Le u gcc Al a gag (31u 330 c ac Hi s gt c ValI ac c Thr ggc G31y g at As p 410 gag (31u cgc Ar g gag (31u g at As p gt c ValI g at As p 300 at c I Ie at g vb t g at As p cgc Ar g cgc Ar g 380 gcc Al a ac c Thr gag (31u c ac Hi s gct Al a 460 ggc G31y 270 gt c ValI t ac Tyr at c I Ie aag Ly s gag (31u 350 ac g Thr at c I Ie gga G31y gcg Al a t ac Tyr 430 gag (31u t ac Tyr 255 aag Ly s ac c Thr t ct Ser ac c Thr at g vb t 335 at c I Ie at c I Ie at c I Ie c ac Hi s c ag O31n 415 cgt Ar g gct Al a at c I Ie ggc O31y g aa O31u gt g ValI gcg Al a 320 aag Ly s g at As p aag Ly s gt c ValI cca Pr o 400 at c I Ie ct g Le u ct c Le u ggc (31y t gc cys gct Al a gt c ValI 305 ac c Thr g at As p at g vb t cca Pr o ct g Le u 385 t ca Ser g aa O31u cct Pr o O31y gt t ValI 465 gct Al a g ac As p 290 ac c Thr ggc O31y c ac Hi s cat Hi s c ag O31n 370 t cc Ser tt t Ph e ct g Le u aag Ly s ggt O31y 450 g ac As p Lys Asn Val 260 gag gct tt c O3 u Al a Phe 275 cca atc aac Pro Ilie Asn gt t gat gag Val Asp (1u aac aag gac Asn Lys Asp 325 gct ct g ct g Al a Leu Leu 340 t cc ct g tt g Ser Leu Leu 355 gt c gac gag Val Asp (1u gaa ggt cgc O31u O31y Ar g gt c at g t cc Val I'b t Ser 405 ttc caa aac Phe 03 n Asn 420 gt t ct c gac Val Leu Asp 435 cag ct c acc O3 n Leu Thr gtt gca ggc Val Al a O31y 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 554 1 557 cca ttc aag ccg gag cac tac cgc tac Pro Phe Lys Pro Ou His Tyr Arg Tyr 470 475 gga <210> 220 <211> 478 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 220 t aat gat t gt cagcat t gag Page 281 seqi i st car r ect ed. t xt l'bt Ala O31n Val l'bt Asp Phe Lys Val Ala Asp Leu Ser 1 5 10 Leu A a 03 u A a vb t Ar g O31u O31y O31u O31y 145 Pr o Le u O31u Le u ValI 225 Ar g O31y cys A a ValI 305 Thr As p Ar g Le u A a Le u Ser ValI Le u 115 O31u ValI O31u O31u ValI 1 95 Hi s As p Ser Ly s O31u 275 Pr o ValI As n A a Ser 355 O31n Ly s Ser A a O31n O31u O31u Pr o Ar g As n 1 65 Le u O31y A a ValI I Ie 245 ValI Ph e As n O31u As p 325 Le u Le u I Ie Ou le Le u 70 As p Pr o Tyr As n O31y 150 As p A a ValI O31u Thr 230 As p Le u As p A a A a 310 li1e O31y Hi s Ar g Ph e Hi s O31y O31u A a Tr p vb t 135 Ar g Ser A a Thr O31u 215 Ly s O31y ValI O31y Le u 295 le le As n Ar g Le u A a 40 vb t A a A a O31y Tr p 120 li1e O31u As p O31u O31u 200 O31y Ser I Ie cys O31n 280 O31n O31u Ser I Ie As p 360 A a As p Thr O31u A a ValI 1 cys Le u Tyr O31u Pr a 1 O31u ValI Ly s As n O31y 265 O31y A a As p Ph e O31y 345 As p O31u O31u ValI ValI A a 90 Pr a I Ie As p O31u Tyr 1 70 O31y Thr Le u Ph e Ar g 250 Tyr A a Le u A a O31u 330 Hi s Tyr O31n O31n Ar g 75 A a ValI As n As p O31n 1 55 li1e Ly s Thr Pr a As p 235 A a O31y Ar g vb t As p 315 O31n Ph e O31u Pr a Thr Tr p I Ie Ph e O31n O31y 140 A a A a Tr p Thr Ph e 220 As n Thr As p ValI As p 300 li1e vb t As p vb t Le u A a A a ValI A a I Ie 125 O31y O31y Ph e O31y O31y 205 Pr a Ly s As p ValI Ly s 285 O31y ValI Le u As n Thr 365 O31y O31y Le u cys O31y Ly s Ser A a ValI O31y 1 li1e Hi s vb t O31y Le u 255 Ly s Thr Ser Thr vb t 335 li1e Le u A a I Ie As n Ser O31y Tr p Thr Pr a 1 vb t A a Ar g As n Thr 240 vb t O31y O31u ValI A a 320 Ly s As p Val Thr Ar g Thr I Ie Lys Page 282 seql i st cor r ect ed. t xt Pro G n Val Asp G u Phe Thr Phe Ser Thr y Arg Ser Ile Ile Val 370 375 380 Leu Ser G u G y Arg Leu Leu Asn Leu G y Asn Ala Thr G y His Pro 385 390 395 400 Ser Phe Val Met Ser Asn Ser Phe Ala Asp G n Thr Ile Ala G n Ile 405 410 415 G u Leu Phe G n Asn G u y G n Tyr G u Asn G u Val Tyr Arg Leu 420 425 430 Pro Lys Val Leu Asp G u Lys Val Ala Arg Ile His Val G u Ala Leu 435 440 445 Gy Gy n Leu Thr u Leu Thr Lys Gu Gn Ala u Tyr Ile y 450 455 460 Val Asp Val Ala G y Pro Phe Lys Pro G u His Tyr Arg Tyr 465 470 475 <210> 221 <211> 128 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA00132 <400> 221 cac gtt gag gct ctc ggc ggt cag ctc acc gaa ctg acc aag gag cag 48 His Val G u Ala Leu G y G y G n Leu Thr G u Leu Thr Lys G u G n 1 5 10 gct gag tac atc ggc gtt gac gtt gca ggc cca ttc aag ccg gag cac 96 Ala G u Tyr lie G y Val Asp Val Ala G y Pro Phe Lys Pro u His 25 t ac cgc tac taatgattgt cagcattgag gga 128 Tyr Arg Tyr <210> 222 <211> <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 222 His Val u Ala Leu y y n Leu Thr u Leu Thr Lys u n 1 5 10 Ala G u Tyr I I e G y Val Asp Val Ala G y Pro Phe Lys Pro u His 25 Tyr Arg Tyr <210> 223 <211> 1396 <212> DNA <213> Cor ynebact er i um gl ut am cum Page 283 seql i st cor r ect ed. t xt <220> <221> CDS <222> (101)..(1396) <223> FRXA01371 <400> 223 aacagcttca atcaattcgg tgtccactcc aacatgtaga tttcctaatt ttcattttct taaaaggagc tcgccaggac gtggtgcgcg ttaaaaaagt gac Asp at t SIle gaa G u at c SIle ttg Leu gat Asp cca Pr o t ac Tyr aac Asn ggt Gy 150 gat Asp gct Al a gt c Val gaa G u aag Lys ctt Leu gca Al a at g Met gct Al a gct Al a ggt Gy tgg Tr p 120 at c SIle gaa G u gat Asp gag G u gag G u 200 ggc Gy gcc Al a gag G u gag G u gt c Val gtt Val gcg Al a cca Pr o at c SIle gac Asp gag G u t ac Tyr 170 ggc Gy acc Thr ct g Leu gat Asp t at Tyr cag G n cag G n cgt Ar g 75 gct Al a gt a Val aac Asn gac Asp cag G n 155 at c SIle aag Lys acc Thr cct Pr o ctt Leu gag G u cct Pr o acc Thr tgg Tr p at c l Ie ttc Phe cag G n ggc Gy 140 gct Al a gca Al a tgg Tr p acc Thr ttc Phe 220 t ca Ser at g let ttg Leu 45 gcc Al a gct Al a gtt Val gcg Al a at c SIle 125 ggt Gy ggt Gy ttc Phe ggc Gy ggt Gy 205 cca Pr o ct a Leu cca Pr o aag Lys gt g Val t cc Ser gt c Val tgg Tr p 110 ttc Phe gac Asp ct g Leu ttg Leu aag Lys 190 gt g Val gcg Al a gca Al a 15 ggt Gy ggc Gy ctt Leu tgc Cys ggc Gy 95 aag Lys agc Ser gcc Al a gtt Val ggc Gy 175 at c SIle cac Hi s at g let gag G u ct c Leu gcc Al a at t SIle aac Asn 80 t cc Ser ggt Gy tgg Tr p acc Thr cca Pr o 160 at g let gct Al a cgc Ar g aac Asn at g let 1 gca Al a at g let cga Ar g gag Gu at t SIle ggc Gy gag G u ggc Gy at g let 145 cca Pr o ct g Leu gag G u ct g Leu gt c Val 225 gca cag gtt Al a G n Val gga Gy cag G n at t I e acc Thr ttc Phe acc Thr t ca Ser gat Asp 130 gct Al a gca Al a cgt Ar g gcc Al a t ac Tyr 210 aac Asn cac Hi s cgc Ar g ggt G y act Thr acc Thr gaa G u 100 gag G u ct g Leu at t l Ie gcc Al a gtt Val 180 aag Lys ttc Phe gct Al a at g let cag G n aag Lys tct Ser gct Al a cag G n gag G u gag G u cca Pr o cgc Ar g aac Asn 165 ctt Leu ggt G y gct Al a gtc Val Page 284 seql i st cor r ect ed. t xt acc aag tcc aag ttt Thr Lys Ser Lys Phe 230 gac Asp ctt Leu gac Asp gct Al a gcc Al a 310 at c SIle ggc Gy cac Hi s ttc Phe ct g Leu 390 aac Asn gaa G u at c SIle tgc Cys cag G n 280 cag G n gag G u t cc Ser at c SIle gac Asp 360 ttc Phe aac Asn ttc Phe cag G n cgc Ar g 250 t ac Tyr gct Al a ct g Leu gcc Al a gag Gu 330 cac Hi s gt c Val acc Thr ggc Sy gat Asp 410 gag G u gat Asp 235 gcc Al a ggc G y cgc Ar g at g Met gac Asp 315 cag G n ttt Phe acc Thr ggt Sy aac Asn 395 cag G n aac Asn act Thr gat Asp gt c Val gat Asp 300 at c SIle at g Met gat Asp cgc Ar g cgc Ar g 380 gcc Al a acc Thr gag G u aac aag tac Asn Lys Tyr acc cgc cac tcc Thr Arg His Ser at g NMbt ggc G y 270 gt c Val t ac Tyr at c SIle aag Lys gag G u 350 acg Thr at c SIle gga Sy gcg Al a t ac Tyr 430 240 at g NMbt ggc G y gaa G u gt g Val gcg Al a 320 aag Lys gat Asp aag Lys gt c Val cca Pr o 400 at c l Ie ct g Leu ggc Gy tgc Cys gct Al a gt c Val 305 acc Thr gat Asp at g Met cca Pr o ct g Leu 385 t ca Ser gaa G u ggc Gy gct Al a gac Asp 290 acc Thr ggc Gy cac Hi s cat Hi s cag G n 370 t cc Ser ttt Phe ct g Leu aag Lys gag G u 275 cca Pr o gtt Val aac Asn gct Al a t cc Ser 355 gt c Val gaa G u gt c Val ttc Phe ct g at c Leu II e 245 aac gt g Asn Val 260 gct ttc Al a Phe atc aac I e Asn gat gag Asp u aag gac Lys Asp 325 ctg ct g Leu Leu 340 ctg ttg Leu Leu gac gag Asp u ggt cgc G y Arg atg t cc bet Ser 405 caa aac Sn Asn 420 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1396 <210> 224 <211> 432 <212> PRT <213> Corynebacter i um gl <400> 224 bet Ala n Val bet Asp 1 5 Al a G y Arg His n lie IVet G n Leu Arg Lys G u ut ari cum Phe Lys Val Ala Asp Leu Ser Leu Ala G u 10 Arg Leu Ala G u Tyr G u IVet Pro y Leu 25 Phe Ala Asp G u n Pro Leu Lys Oy Ala 40 Page 285 Ar g (31u li1e O31y O31u O31y vb t 145 Pr a Le u O31u Le u ValI 225 Ar g G31y Cys A a ValI 305 Th r As p vb t Pr a Le u 385 Ser IlieAla Gy Ser Ilie His [Vb 55 seqI i st car r ect ed. t xt t Thr Val O31n Thr A a Val Leu I Ie Le u Ser ValI Le u 115 O31u ValI O31u O31u ValI 1 95 Hi s As p Ser Lys5 O31u 275 Pr a ValI As n A a Ser 355 ValI O31u ValI A a O31n O31u O31u Pr a Ar g As n 1 65 Le u G31y A a ValI I Ie 245 ValI Ph e As n O31u As p 325 Le u Le u O31u Ar g Ser 405 Le u 70 As p Pr a Ty r As n G31y 150 As p A a ValI O31u Th r 230 As p Le u As p A a A a 310 li1e G31y Hi s Ph e Le u 390 As n G31y O31u A a Tr p vb t 135 Ar g Ser A a Th r O31u 215 Lys5 G31y ValI G31y Le u 295 le le As n Ar g Th r 375 Le u Ser A a A a G31y Tr p 120 li1e O31u As p O31u O31u 200 G31y Ser I Ie Cys O31n 280 O31n O31u Ser I Ie As p 360 Ph e As n Ph e O31u A a ValI 1 05 Cys Le u Ty r O31u Pr a 1 85 O31u ValI Lys5 As n G31y 265 G31y A a As p Ph e G31y 345 As p Ser Le u A a ValI A a 90 Pr a I Ie As p O31u Ty r 1 70 G31y Thr Leu Ph e Ar g 250 Ty r A a Le u A a O31u 330 Hi s ValI Th r G31y As p 410 Ar g 75 A a ValI As n As p O31n 1 55 li1e Lys5 Th r Pr a As p 235 A a G31y Ar g vb t As p 315 O31n Ph e Th r O31y As n 395 Tr p I Ie Ph e O31n O31y 140 A a A a Tr p Th r Ph e 220 As n Th r As p ValI As p 300 li1e vb t As p Ar g Ar g 380 A a A a ValI A a I Ie 125 O31y O31y Ph e O31y O31y 205 Pr a Lys5 As p ValI Ly s 285 O31y ValI Le u As n Th r 365 Ser Th r Ser ValI Tr p 110 Ph e As p Le u Le u Ly s 1 ValI A a Ty r vb t O31y 270 ValI Ty r I Ie Ly s O31u 350 Th r I Ie O31y Cys O31y Ly s Ser A a ValI O31y 1 li1e Hi s vb t O31y Le u 255 Ly s Th r Ser Th r vb t 335 le le le Hi s As n Ser O31y Tr p Th r Pr a 1 vb t A a Ar g As n Th r 240 vb t O31y O31u ValI A a 320 Ly s As p Ly s ValI Pr a 400 On Thr IieAia(3n Ilie 415 Page 286 seqi i st car r ect ed. t xt Ou Leu Phe On Asn Ou Oy On Tyr Ou Asn Ou Val Tyr Arg Leu 420 425 430 <210O> 225 <21 1> 2358 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2335) <223> RXN02085 <400> 225 cacccggt ga t t tcgcgaac ct tgaaacat cgt cagaaga gat ccgcacg t tcggct caa gcagaaagt c t t taact cac ttgccgtgcg tcctagccgg atg act tcc aac ttt 11! l'bt Thr Ser Asn Phe t ct Ser aag Ly s ct t Le u t ct Ser at g vb t g ac As p t ac Tyr ac c Thr gcg A a cgt Ar g 1 50 cca Pr o act Thr gcg A a c ag O31n tt g Le u g at As p gct A a ggc G31y t gg Tr p 120 ac a Thr c ag O31n act Thr gct A a g aa O31u gcc A a t cc Ser gcc A a cat Hi s gct A a g at As p tt c Ph e gt t ValI ct t Le u 1 70 ggt G31y ggc G31y cgc Ar g gt t ValI gct A a 75 gaa O31u cgc Ar g acc Thr gt t ValI cgt Ar g 155 t cc Ser ct t Le u t ac Tyr c aa O31n ccg Pr o at t I Ie aac As n ggt O31y aac As n tt g Le u 140 ggc O31y ct t Le u cgc Ar g aat As n gt c ValI gca A a ggt O31y ggt O31y gag O31u 110 c ac Hi s gcg A a aat As n cgc Ar g at c lIe 15 gga O31y aac As n gga O31y gt g ValI ct c Le u 95 ac c Thr t ac Tyr t cc Ser gcc A a ac c Thr 1 75 gga O31y t ca Ser act Thr cgt Ar g ct g Le u cca Pr o ct g Le u ct c Le u gcg A a cgc Ar g 1 act Thr 1 gcg A a at t I Ie gca A a t cc Ser ccg Pr o ct g Le u cct Pr a gt g ValI ct g Le u 145 cct Pr a g at As p aag Ly s g aa O31u t cg Ser t ac Tyr gag O31u t gg Tr p gca A a ccg Pr a 130 at t I Ie gt t ValI ggt (31y g aa O31u cgc Ar g t ct Ser g ac As p tt t Ph e g ac As p 1 00 gca A a tt g Le u g at As p gt t ValI aat As n 1 Page 287 seql i st cor r ect ed. t xt ttg Leu t ct Ser gat Asp ttg Leu 230 gat Asp gtt Val gag Gu acc Thr ggc Gy 310 acc Thr ttc Phe gcc Al a gct Al a cct Pr o 390 aag Lys cca Pr o t cc Ser cac Hi s gat Asp 200 gct Al a aag Lys gcg Al a ttg Leu ct g Leu 280 ct g Leu at c SIle gag Gu tcg Ser aac Asn 360 cga Ar g cgt Ar g ct g Leu acc Thr act Thr 440 cct gca ct g Pro Al a Leu gag G u gag G u gat Asp aac Asn 250 acc Thr gtt Val gct Al a gt g Val gag Gu 330 aag Lys gac Asp acc Thr cgt Ar g ctt Leu 410 t cc Ser gag G u tgg Tr p gtt Val ggc G y 235 act Thr cat Hi s gcg Al a gct Al a tct Ser 315 aac Asn at c SIle gcg Al a t cc Ser gga G y 395 cca Pr o at t SIle cag G n gtt Val ttg Leu 220 gt g Val ctt Leu ggc G y ggc Gy ctt Leu 300 acc Thr at t SIle acc Thr gct Al a cca Pr o 380 t cc Ser gct Al a cgt Ar g t ac Tyr ttt gag gtc Phe G u Val 190 cag at c gat G n Ile Asp 205 gag cag gt c G u G n Val ttt gtc aat Phe Val Asn gcg ggc at c Al a G y li e 255 gt c act gag Val Thr G u 270 atc gtt gat Ile Val Asp 285 gct tcc ct g Al a Ser Leu tct tgt tca Ser Cys Ser gag cct gag G u Pro G u 335 gag gtc aag G u Val Lys 350 gcg ttc gat Al a Phe Asp 365 cgc acc gca Arg Thr Ala ttc gac act Phe Asp Thr ct g cca acc Leu Pro Thr 415 t ct gct cgc Ser Al a Ar g 430 gaa gag gca G u G u Al a 445 tac gag Tyr G u gag cct Su Pro cgc gct Arg Ala 225 act tac Thr Tyr 240 ggc ctt G y Leu ctt gct Leu Al a ggt cgt G y Arg aag cgc Lys Arg 305 ctg ct g Leu Leu 320 gtc cgc Val Arg ctg ctt Leu Leu gcg gcg Al a Al a cca at c Pro Ile 385 cgt gtt Arg Val 400 acc acc Thr Thr gct cgt Al a Ar g atg cgc IVet Ara cgc ct c at c Arg Leu II e 195 gcg ttg gtc Al a Leu Val 210 ggt t ac acc G y Tyr Thr ttc ggc tct Phe G y Ser ggc gcg att G y Al a li e 260 gcg tgg aag Al a Trp Lys 275 aac att tgg Asn lle Trp 290 ct g gca gct Leu Al a Al a cac gtt cct His Val Pr o gac tgg ctt Asp Trp Leu 340 gcc gac gcc Al a Asp Al a 355 t cc gca gca Ser Al a Al a 370 acg cag gaa Thr G n u acg ctg cag Thr Leu G n att ggt tct I e G y Ser 420 ctg cgc aag Leu Arg Lys 435 aag Lys acc Thr act Thr ggc G y 245 ggc G y ggt G y cgc Ar g cgc Ar g t ac Tyr 325 gcc Al a ct a Leu at t SIe ct c Leu gag G u 405 ttc Phe gaa G u 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 gaa gaa atc gat G u G u lie Asp 450 Page 288 seql i st cor r ect ed. t xt ct g Leu ggt Sy 470 gac Asp cgc Ar g cca Pr o cat Hi s ttc Phe 550 ct g Leu at c SIle gac Asp ct g Leu tgc Cys 630 gcc Al a gct Al a tgg Tr p ct c Leu aac Asn 710 at c SIle cca Pr o ttc Phe gtt Val act Thr 520 aag Lys cgc Ar g ct g Leu cag G n gat Asp 600 act Thr t cc Ser gt c Val ct g Leu at c SIle 680 gag G u gac Asp aag Lys cgc Ar g t ca Ser 490 cct Pr o aag Lys at g Met gat Asp gat Asp 570 gat Asp cct Pr o ggc Gy ttc Phe acc Thr 650 tct Ser t cc Ser gca Al a ggt Sy cag gaa gaa ctt Sn G u G u Leu 460 aac gac atg gtt Asn Asp Ibet Val 475 acc gcc aac ggc Thr Al a Asn G y cca gtg ttg ttc Pro Val Leu Phe 510 tgg ttc cag tac Trp Phe G n Tyr 525 ctc acc ggt cca Leu Thr y Pro 540 cag ccg ctg gct G n Pro Leu Ala 555 gaa att aac gat G u Ile Asn Asp gag cct gcg att Su Pro Ala lle 590 gcc tac ctg cag Al a Tyr Leu G n 605 gca ccc gac gac Ala Pro Asp Asp 620 aac gaa gt g at c Asn G u Val lle 635 at c gaa gca gca I e G u Al a Al a tcc ggc ttc gag Ser G y Phe G u 670 ccg cgc gtt cct Pro Arg Val Pro 685 ctg cag tcc gtg Leu G n Ser Val 700 ctg aag acc cgt Leu Lys Thr Arg 715 ggt ctt gat G y Leu Asp 465 cag tac ttc G n Tyr Phe 480 tgg gtc caa Trp Val n 495 gga aac gtt G y Asn Val gca cag agc Al a G n Ser gt c acc at c Val Thr Ile 545 acc act gct Thr Thr Al a 560 ct c at c gag Leu Ile G u 575 cgt gaa ct g Arg G u Leu tgg tcc gtg Trp Ser Val gt c caa at c Val Gn lle 625 t cc tcg gt c Ser Ser Val 640 cgt t cc gac Arg Ser Asp 655 ct c ggc gt c Leu G y Val tcc gcg cag Ser Al a G n gat cct cgc Asp Pro Arg 705 gga tgg cca G y Trp Pr o 720 Page 289 gt g Val t ct Ser agc Ser t cc Ser ct g Leu 530 ctt Leu gac Asp gct Al a ttg Leu gac Asp 610 cac Hi s at c SIle at g Met gga G y aaa Lys 690 cag G n gaa G u ttg gtt cac Leu Val Hi s gaa ct t ct c G u Leu Leu 485 t ac ggc t cc Tyr G y Ser 500 cgc cca gcg Ar g Pro Al a 515 acc cag aag Thr G n Lys gca t gg t cc Al a Trp Ser cag gtt gca Sn Val Ala 565 ggc gcg aag G y Al a Lys 580 ccg ct a cga Pro Leu Arg 595 tcc ttc cgc Ser Phe Ar g acc cac at g Thr His Met gcg ttg gat Ala Leu Asp 645 cag gtc ct c G n Val Leu 660 cct ggt gt g Pro G y Val 675 gt g gac ggt Val Asp G y ctg tgg gt c Leu Trp Val gt g aa gct Val u Al a 725 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 seqI i st car r ect ed. t xt tcc cta aag gt t ct c gt t gag t cc gct aag cag gct cgt gag aaa at c Ser Leu Lys Val Leu Val Ou Ser Ala Lys On Ala Arg Ou Lys Ilie 730 735 740 gga gca act at c t aaat t gggt t accgct agg aac G31y Al a Th r Ilie 745 <210> 226 <211> 745 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 2323 2358 <400> 226 I'bt Thr Ser Al a Al a Ser Pr o Le u Pr o ValI Le u 145 Pr o As p O31u Pr o Al a 225 Tyr Le u Ar g G31y As p Tyr Ar g I Ie O31n 115 O31u O31u Le u Ser Le u 1 95 Le u Tyr O31y Al a Asn Phe Ser 03 u Leu Lys Ar g O31u Leu Ser Leu Ser Asp Al a [vb t 70 Phe Asp Asp Asp Arg Tyr 1 00 Al a I'b t Thr Leu Ser Al a Asp Leu Arg 150 Val G31y Pr o 1 65 Asn Pro Leu 1 80 Il e Lys Ser Val Thr Asp Thr Thr Leu 230 Ser O31y Asp 245 Il e 03 y Val 260 Ser Ph e Al a G y Le u I Ie Ph e Ly s As p 135 cys Le u As p Ph e ValI 215 Al a 0On As p Thr Al a O31n 40 Le u As p Al a O31y Tr p 120 Thr O31n Thr Hi s As p 200 Al a Ly s Al a Le u ValI Le u 25 Thr As p Thr As p Al a 1 05 Ph e Ar g O31n Ph e Le u 1 85 Thr Pr a Ar g Le u ValI 265 Al a 10 O31u Al a Ser Al a Hi s 90 Al a As p Ph e ValI Le u 1 70 Pr a O31u O31u As p As n 250 Thr O31y O31y Ar g ValI Al a O31u Ar g Thr ValI Ar g 1 Ser Al a Tr p ValI O31y 235 Thr Hi s Pr a Tr p Le u Ph e Le u As p Thr Tyr 125 As p ValI Al a Ph e O31n 205 O31u Ph e Al a ValI Ar g As n ValI Al a O31y O31y O31u 110 Hi s Al a As n Ar g O31u 1 li1e O31n ValI O31y Thr 270 lIe O31y As n G y ValI Le u Thr Tyr Ser Al a Thr 1 ValI As p ValI As n I Ie 255 O31u Ala Ala Trp Lys Oy Ou Ou Leu Leu Val Ala O1y lieVal Asp Oy Page 290 seql i st cor r ect ed. t xt Ar g Ar g 305 Leu Ar g Leu Al a SIle 385 Val Thr Ar g Ar g Asp 465 Phe G n Val Ser SIle 545 Al a G u Leu Val SIle 625 275 I le Al a Val Tr p Asp 355 Al a G n Leu Gy Ar g 435 G u Leu G u Tyr Ar g 515 Thr Al a G n Gy Pr o 595 Ser Thr 280 Leu Cys Ala Ala 285 Ala Ser Leu Lys Arg Thr Arg G y 310 Tyr Thr 325 Al a Phe Leu Al a I e Al a Leu Pro 390 G u Lys 405 Phe Pro Su Ser Asp Leu Hi s G y 470 Leu Asp 485 Ser Arg Al a Pr o Lys Hi s Ser Phe 550 Al a Leu 565 Lys II e Arg Asp Arg Leu let Cys 630 Asp 295 Pr o Leu Sy Sy Ser 375 Sy Ser G n SIle Val 455 G u Sy Cys let Val 535 Val Al a SIle Val Al a 615 Tyr Al a Al a G u 345 I le Ar g Ser G u Pr o 425 Leu Al a G u Leu Ar g 505 Val Sy Asp Ar g Val 585 Lys Al a G u Ser 315 Asn SIle Al a Ser Sy 395 Pr o SIle G n G n Asn 475 Thr Pr o Tr p Leu G n 555 G u G u Al a Al a Leu 300 Thr SIle Thr Al a Pr o 380 Ser Al a Ar g Tyr G u 460 Asp Al a Val Phe Thr 540 Pr o SIle Pr o Tyr Pr o 620 Ser G u G u Al a 365 Ar g Phe Leu Ser G u 445 G u let Asn Leu G n 525 Sy Leu Asn Al a Leu 605 Asp Ser G u 335 Lys Asp Al a Thr Thr 415 Ar g Al a Sy G n Tr p 495 Sy Al a Val Thr Leu 575 Ar g Tr p Val Leu 320 Val Leu Al a Pr o Ar g 400 Thr Al a let Leu Tyr 480 Val Asn G n Thr Thr 560 I e G u Ser G n Phe Asn G u Val Ile Ser Ser 635 640 Page 291 ValI As p ValI O31n Ar g 705 Pr o A a A a O31n Pr o 675 ValI Le u ValI aOU As p 645 Le u ValI G31y ValI A a 725 li1e A a A a Tr p Le u As n 71 0 Ser G31y As p A a As p Le u 695 Pr o Le u A a seql i st car r ect ed. t xt Val Thr Th r IIe O31u Ala 650 Leu Lys Ser Ser 03 y Phe 665 li1e Hi s Ser Pr o Ar g Val 680 685 03 u A a A a Leu 03 n Ser 700 As p Cys 03 y Leu Lys Thr 715 Lys Val Leu Val 03 u Ser 730 Thr I Ie 745 A a O31u 670 Pr o ValI Ar g A a Ar g 655 Le u Ser As p G31y 735 <210O> 227 <21 1> 1923 <212> DNA <213> Cor ynebact er i urn gi ut ari cumn <220> <221 CDS <222> (1900) <223> FRXA02085 <400> 227 cacccggt ga t t tcgcgaac ct tgaaacat cgt cagaaga gat ccgcacg t tcggct caa gcagaaagt c t t taact cac ttgccgtgcg tcctagccgg t ct Ser aag ct t Le u t ct Ser at g vb t g ac As p t ac Ty r act Th r gcg A a c ag O31n tt g Le u g at As p gct A a ggc G31y gct A a g aa (31u gcc A a t cc Ser gcc A a cat Hi s gct A a ggt G31y ggc G31y cgc Ar g gt t ValI gct A a 75 gaa (31u cgc Ar g ct t Le u t ac Ty r c aa O31n ccg Pr a at t I Ie aac As n ggt (31y cgc Ar g aat As n gt c ValI gca A a ggt O31y ggt O31y gag O31u 110 at c lIe 15 gga O31y aac As n gga O31y gt g ValI ct c Le u 95 ac c Th r gga O31y t ca Ser act Th r cgt Ar g ct g Le u 80 cca Pr a ct g Le u at g vb t 1 gcg A a at t I Ie gca A a t cc Ser ccg Pr a ct g Le u cct Pr a aag Lys5 g aa O31u t cg Ser t ac Ty r gag O31u t gg Tr p gca A a act t cc aac Thr Ser Asn g aa O31u cgc Ar g t ct Ser g ac As p tt t Ph e g ac As p 1 00 gca A a tt t Ph e ct g Le u g aa O31u tt g Le u gca A a g at As p cgc Ar g at g vb t acc aagtggttt gat acc aactac cactac ctc gtg ccg gagttgtct Page 292 Thr Lys Tr p 120 Phe Asp Thr Asn Ty 12 seql i st cor r ect ed. t xt r His Tyr Leu Val Pro G u Leu Ser 5 gcg Al a cgt Ar g 150 cca Pr o ttg Leu tct Ser gat Asp ttg Leu 230 gat Asp gtt Val gag G u acc Thr ggc Gy 310 acc Thr ttc Phe gcc Al a gct Al a aca Thr cag G n act Thr cac Hi s gat Asp 200 gct Al a aag Lys gcg Al a ttg Leu ct g Leu 280 ct g Leu at c SIle gag G u tcg Ser aac Asn 360 cga Ar g ttc Phe gtt Val ctt Leu 170 cct Pr o gag G u gag G u gat Asp aac Asn 250 acc Thr gtt Val gct Al a gt g Val gag G u 330 aag Lys gac Asp acc Thr gtt Val cgt Ar g 155 t cc Ser gca Al a tgg Tr p gtt Val ggc G y 235 act Thr cat Hi s gcg Al a gct Al a tct Ser 315 aac Asn at c SIle gcg Al a t cc Ser ttg Leu 140 ggc Gy ctt Leu ct g Leu gtt Val ttg Leu 220 gt g Val ctt Leu ggc G y ggc G y ctt Leu 300 acc Thr at t SIle acc Thr gct Al a cca Pr o 380 gat Asp gtt Val gct Al a ttt Phe cag G n 205 gag G u ttt Phe gcg Al a gt c Val at c SIle 285 gct Al a tct Ser gag G u gag G u gcg Al a 365 cgc Ar g gcg Al a aat Asn cgc Ar g gag G u 190 at c SIle cag G n gt c Val ggc G y act Thr 270 gtt Val t cc Ser tgt Cys cct Pr o gt c Val 350 ttc Phe acc Thr t cc Ser gcc Al a acc Thr 175 gt c Val gat Asp gt c Val aat Asn at c SIle 255 gag G u gat Asp ct g Leu t ca Ser gag G u 335 aag Lys gat Asp gca Al a gcg Al a cgc Ar g 160 act Thr t ac Tyr gag G u cgc Ar g act Thr 240 ggc G y ctt Leu ggt G y aag Lys ct g Leu 320 gt c Val ct g Leu gcg Al a cca Pr o ct g Leu 145 cct Pr o gat Asp gag G u cct Pr o gct Al a 225 t ac Tyr ctt Leu gct Al a cgt Ar g cgc Ar g 305 ct g Leu cgc Ar g ctt Leu gcg Al a at c SIle 385 130 at t SIle gtt Val ggt Gy cgc Ar g gcg Al a 210 ggt Gy ttc Phe ggc G y gcg Al a aac Asn 290 ct g Leu cac Hi s gac Asp gcc Al a t cc Ser 370 acg Thr gag G u ct g Leu t cc Ser ct c Leu 195 ttg Leu t ac Tyr ggc G y gcg Al a tgg Tr p 275 at t SIle gca Al a gtt Val tgg Tr p gac Asp 355 gca Al a cag G n gat Asp gtt Val aat Asn 180 at c SIle gt c Val acc Thr tct Ser at t l Ie 260 aag Lys tgg Tr p gct Al a cct Pr o ctt Leu 340 gcc Al a gca Al a gaa G u ct c Leu ggt G y 165 cct Pr o aag Lys acc Thr act Thr ggc G y 245 ggc G y ggt G y cgc Ar g cgc Ar g t ac Tyr 325 gcc Al a ct a Leu at t SIe ct c Leu 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 cct ggc cgt agc cgt gga tcc ttc gac act cgt gtt acg ctg cag gag Page 293 seqI i st car r ect ed. t xt Pro Oy Arg Ser Arg Oy Ser Phe Asp Thr Arg Val Thr 390 395 400 Leu G3 n G3 u 405 aag Ly s cca Pr o t cc Ser ct g Le u ggt O31y 470 g ac As p cgc Ar g cca Pr o cat Hi s tt c Ph e 550 ct g Le u at c I Ie ag a Ar g ct g Le u ac c Thr act Thr 440 at c I Ie cca Pr o tt c Ph e gt t ValI act Thr 520 aag Ly s cgc Ar g ct g Le u c ag O31n cga Ar g 600 ct t Le u 410 t cc Ser gag O31u aag Ly s cgc Ar g t ca Ser 490 cct Pr o aag Ly s at g vb t g at As p g at As p 570 g at As p cca Pr a at t I Ie cag O31n cag O31n aac As n 475 acc Thr cca Pr a t gg Tr p ct c Le u cag O31n 555 gaa O31u gag (31u gct A a cgt Ar g t ac Tyr g aa O31u 460 g ac As p gcc A a gt g ValI tt c Ph e ac c Thr 540 ccg Pr a at t I Ie cct Pr a ct g Le u t ct Ser g aa O31u 445 g aa O31u at g vb t aac As n tt g Le u c ag O31n 525 ggt O31y ct g Le u aac As n gcg A a cca Pr a gct A a 430 gag O31u ct t Le u gt t ValI ggc O31y tt c Ph e 510 t ac Tyr cca Pr a gct A a g at As p at t I Ie 590 ac c Thr 415 cgc Ar g gca A a ggt O31y c ag O31n t gg Tr p 495 gga O31y gca A a gt c ValI ac c Thr ct c Le u 575 cgt Ar g ac c Thr gct A a at g vb t ct t Le u t ac Tyr 480 gt c ValI aac As n c ag O31n ac c Thr act Thr 560 at c I Ie g aa O31u at t I Ie ct g Le u g aa O31u 450 gt g ValI t ct Ser ag c Ser t cc Ser ct g Le u 530 ct t Le u g ac As p gct A a tt g Le u ggt O31y cgc Ar g 435 g aa O31u tt g Le u g aa O31u t ac Tyr cgc Ar g 515 ac c Thr gca A a c ag O31n O31y ccc Pr a 595 t ct Ser 420 aag Ly s at c I Ie gt t ValI ct t Le u ggc O31y 500 cca Pr a c ag O31n t gg Tr p gt t ValI gcg A a 580 gct A a 1363 1411 1459 1 507 1 555 1 603 1651 1 699 1 747 1 795 1 843 1891 1 923 t aagcct gcc t acct gcagt ggt <210O> 228 <21 1> 600 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 228 I'bt Thr Ser Asn Phe Ser Ser Thr Val Ala O1y Leu Pro Arg Ilie Oy 1 5 10 Ala Lys Ar g O31u Leu Lys Phe Ala Leu O31u Oy Tyr Trp Asn Oy Ser 25 Page 294 le 31u G31y Ar g (3 u L eu A a G3 4 seqi i st car r ect ed. t xt n Thr Ala Ar g O31n Leu Val Asn Thr 0 A a Ser Pr o Le u Pr o ValI Le u 145 Pr o As p O31u Pr o A a 225 Tyr Le u A a Ar g Ar g 305 Le u Ar g Le u A a I Ie 385 As p Tyr Ar g I Ie O31n 115 O31u O31u Le u Ser Le u 1 95 Le u Tyr G31y A a Tr p 275 li1e A a ValI Tr p As p 355 A a (31n Le u A a As p Ar g vb t Ser Le u G31y 1 65 Pr a Ly s Thr Thr G31y 245 G31y O31y Ar g Ar g Tyr 325 A a Le u I Ie Le u Ser vb t 70 As p Tyr Thr A a Ar g 150 Pr a Le u Ser As p Le u 230 As p ValI O31u Thr G31y 310 Thr Ph e A a A a Pr a 390 G31y Le u I Ie Ph e Ly s As p 135 cys Le u As p Ph e ValI 215 A a O31n As p O31u As p 295 Pr a Le u G31y G31y Ser 375 (31y Le u As p A a G31y Tr p 120 Thr O31n Thr Hi s As p 200 A a Ly s A a Le u Le u 280 Le u I Ie O31u Ser As n 360 Ar g Ar g As p Thr As p A a 1 05 Ph e Ar g O31n Ph e Le u 1 85 Thr Pr a Ar g Le u ValI 265 Le u cys A a A a O31u 345 li1e Ar g Ser Ser A a Hi s 90 A a As p Ph e ValI Le u 1 70 Pr a O31u O31u As p As n 250 Thr ValI A a ValI O31u 330 Ly s As p Thr ValI A a O31u Ar g Thr ValI Ar g 1 Ser A a Tr p ValI O31y 235 Thr Hi s A a A a Ser 315 As n I Ie A a Ser Pr a li1e As n O31y As n Le u 140 O31y Le u Le u ValI Le u 220 ValI Le u O31y O31y Le u 300 Thr I Ie Thr A a Pr a 380 Ph e Le u As p Thr Tyr 125 As p ValI A a Ph e O31n 205 O31u Ph e A a ValI I Ie 285 A a Ser O31u O31u A a 365 Ar g A a O31y O31y O31u 110 Hi s A a As n Ar g O31u 1 li1e O31n ValI O31y Thr 270 ValI Ser cys Pr a ValI 350 Ph e Thr O31y ValI Le u Thr Tyr Ser A a Thr 1 ValI As p ValI As n I Ie 255 O31u As p Le u Ser O31u 335 Ly s As p A a Ar g G31y 395 Ser Phe Asp Thr Page 295 seql i st cor r ect ed. t xt Val Thr Leu n G u Lys Ser Leu G u Leu Pro Ala Leu Pro Thr Thr 405 Thr Ar g Ar g Asp 465 Phe G n Val Ser SIle 545 Al a G u Leu Gy Ar g 435 G u Leu G u Tyr Ar g 515 Thr Al a G n Sy Pr o 595 Phe G u Asp Hi s Leu 485 Ser Al a Lys Ser Al a 565 Lys Thr G n SIle Val 455 G u Sy Cys et Val 535 Val Al a SIle Ar g Pr o 425 Leu Al a G u Leu Ar g 505 Val Gy Asp Ar g Val 585 SIle G n G n Asn 475 Thr Pr o Tr p Leu G n 555 G u G u Ser G u 445 G u Met Asn Leu G n 525 Gy Leu Asn Al a Al a 430 G u Leu Val Sy Phe 510 Tyr Pr o Al a Asp l Ie 590 415 Arg Ala Al a Met G y Leu G n Tyr 480 Trp Val 495 Sy Asn Al a n Val Thr Thr Thr 560 Leu II e 575 Arg G u <210> 229 <211> 603 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(580) <223> FRXA02086 <400> 229 gatgatcagc cgctggctac cactgctgac caggttgcac tggcactgcg cgatgaaatt aacgatctca tcgaggctgg cgcgaagatc atccaggtgg atg agc ctg cga ttc IVet Ser Leu Arg Phe 1 gtg aac tgt tgc ccg cta cga gac gtc gat aag cct gcc tac ctg cag Val Asn Cys Cys Pro Leu Arg Asp Val Asp Lys Pro Ala Tyr Leu G n 15 tgg tcc gtg gac tcc ttc cgc ctg gcg act gcc ggc gca ccc gac gac Trp Ser Val Asp Ser Phe Arg Leu Ala Thr Ala y Ala Pro Asp Asp 30 gtc caa atc cac acc cac atg tgc tac tcc gag ttc aac gaa gtg atc Val n Ile His Thr His Met Cys Tyr Ser u Phe Asn u Val lie Page 296 45 tcc tcg gtc atc gcg ttg gat gcc g Ser Ser Val Ile Ala Leu Asp Ala A 60 cgt tcc gac atg cag gtc ctc gct g Arg Ser Asp Vet G n Val Leu Ala A 75 ctc ggc gtc gga cct ggt gtg tgg g Leu y Val y Pro G y Val Trp A tcc gcg cag aaa gtg gac ggt ctc c Ser Ala G n Lys Val Asp y Leu L 105 1 gat cct cgc cag ctg tgg gtc aac c Asp Pro Arg G n Leu Trp Val Asn F 120 125 gga tgg cca gaa gtg gaa gct tcc c Sy Trp Pro G u Val G u Ala Ser L 135 140 aag cag gct cgt gag aaa atc gga g Lys n Ala Arg u Lys lie y A 150 155 aac <210> 230 <211> 160 <212> PRT <213> Cor ynebact eri um gl ut am cum 1 i st cor r ect ed. t xt iat gtc acc acc atc gaa gca gca 307 sp Val Thr Thr Ile u Ala Ala ct ctg aaa tct tcc ggc ttc gag 355 l1 a Leu Lys Ser Ser G y Phe G u 80 iat atc cac tcc ccg cgc gtt cct 403 sp lie His Ser Pro Arg Val Pro 95 100 t c gag gct gca ctg cag tcc gtg 451 .eu G u Ala Ala Leu G n Ser Val 10 115 ca gac tgt ggt ctg aag acc cgt 499 'ro Asp Cys y Leu Lys Thr Arg 130 t a aag gtt ctc gtt gag tcc gct 547 .eu Lys Val Leu Val G u Ser Ala 145 ica act atc taaattgggt taccgctagg 600 l a Thr Ile 160 603 <400> 23 IVet Ser 0 Leu Arg Phe Val Asn Cys Cys 1 Pr o Sy Phe Thr Ser Ser Al a Sy Leu 145 Tyr Pr o G u G u Gy Ar g G n 115 Lys G u 5 G n Asp SIle Al a G u Pr o Val Ar g Al a Ser G n Ser Ser Sy Al a Pr o Tr p 135 G n Asp Hi s SIle Mbet Sy Lys 105 G n G u Ar g Pro Leu Arg 10 Ser Phe Ar g Thr His bet Ala Leu Asp G n Val Leu 75 Pro G y Val 90 Val Asp G y Leu Trp Val Val u Ala 140 G u Lys Ile 155 Page 297 Asp Leu Cys Al a Al a Tr p Leu Asn 125 Ser Gy Val Al a Tyr Asp Al a Asp Leu 110 Pr o Leu Al a Asp Thr Ser Val Leu SIle G u Asp Lys Thr Lys Al a G u Thr Lys Hi s Al a Cys Val SIe 160 seql i st cor r ect ed. t xt <210> <211> <212> <213> <220> <221> <222> <223> 231 1326 DNA Cor ynebact eri um gl ut ai cum CDS (1303) RXN02648 <400> 231 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa gagtttgata ctttctttcg acttttagat tggattttca acgcggttag agtcgaatga agc cag aac Ser G n Asn atc agg acc act Ile Arg Thr Thr cac Hi s gtt ggt tcc ttg Val G y Ser Leu cgt acc cca gag Arg Thr Pro G u ct a ct t Leu Leu gat gca aac atc aag cgt tct aac ggt gag att ggg gag gag gaa ttc Asp Al a Asn Ile Lys Arg Ser Asn Gy Gu lie Gy Gu Gu Gu Phe 30 ttc cag att Phe n II e ctg cag tct tct Leu G n Ser Ser gt a Val 45 gat gac gtg atc Asp Asp Val lI e aag Lys cgc cag gtt Arg n Val gac ctg Asp Leu ggt atc gac atc Sy Ile Asp Ile ctt Leu aac gag ggc gaa Asn u G y u ggc cac gtc acc G y His Val Thr t cc Ser ggt gca gtt gac Sy Ala Val Asp ggt gca tgg tgg Sy Ala Trp Trp aac Asn tac tcc ttc acc Tyr Ser Phe Thr ctg ggc gga ctg Leu G y G y Leu acc Thr atg acc gat acc IVet Thr Asp Thr gac cgt tgg gca agc cag gaa Asp Arg Trp Ala Ser n G u 95 100 gca gtg cgt Ala Val Arg cgt cgc gac Arg Arg Asp 120 acc cct ggc aac Thr Pro Gy Asn at c SIle 110 gag ctg acc agc G u Leu Thr Ser ttc tct gat Phe Ser Asp 115 cca gt a t ct Pro Val Ser cgc gca ttg ttc Arg Ala Leu Phe gaa gca tac gag G u Al a Tyr G u gat Asp 130 ggc atc G y lle 135 ttc acc ggt cgc Phe Thr y Arg gct Al a 140 tct gtg ggc aac Ser Val Gy Asn cca Pr o 145 gag ttc acc gga G u Phe Thr G y cct Pr o 150 att acc tac att Ile Thr Tyr Ile cag gaa gaa act Sn u G u Thr cag G n 160 acg gat gtt gat Thr Asp Val Asp ctg aag aag ggc Leu Lys Lys G y at g Met 170 aac gca gcg gga Asn Al a Al a G y gct Al a 175 acc gac ggc ttc Thr Asp G y Phe gtt gca Val Ala 180 gca cta tcc cca gga tct gca gct cga Ala Leu Ser Pro y Ser Ala Ala Arg ttg acc aac aag ttc tac gac Leu Thr Asn Lys Phe Tyr Asp Page 298 seql i st cor r ect ed. t xt 185 190 195 act gat gaa gaa gtc gtc gca gca tgt gct gat gcg ctt tcc cag gaa 739 Thr Asp G u Gu Val Val Ala Ala Cys Ala Asp Ala Leu Ser Gn u 200 205 210 tac aag atc atc acc gat gca ggt ctg acc gtt cag ctc gac gca ccg 787 Tyr Lys lie lie Thr Asp Ala y Leu Thr Val G n Leu Asp Ala Pro 215 220 225 gac ttg gca gaa gca tgg gat cag atc aac cca gag cca agc gtg aag 835 Asp Leu Ala G u Ala Trp Asp n Ile Asn Pro G u Pro Ser Val Lys 230 235 240 245 gat tac ttg gac tgg atc ggt aca cgc atc gat gcc atc aac agt gca 883 Asp Tyr Leu Asp Trp lie y Thr Arg lie Asp Ala lie Asn Ser Ala 250 255 260 gtg aag ggc ctt cca aag gaa cag acc cgc ctg cac atc tgc tgg ggc 931 Val Lys G y Leu Pro Lys G u G n Thr Arg Leu His lie Cys Trp G y 265 270 275 tct tgg cac gga cca cac gtc act gac atc cca ttc ggt gac atc att 979 Ser Trp His G y Pro His Val Thr Asp lie Pro Phe G y Asp lie lie 280 285 290 ggt gag atc ctg cgc gca gag gtc ggt ggc ttc tcc ttc gaa ggc gca 1027 G y u Ile Leu Arg Ala u Val y G y Phe Ser Phe G u y Ala 295 300 305 tct cct cgt cac gca cac gag tgg cgt gta tgg gaa gaa aac aag ctt 1075 Ser Pro Arg His Ala His G u Trp Arg Val Trp G u G u Asn Lys Leu 310 315 320 325 cct gaa ggc tct gtt atc tac cct ggt gtt gtg tct cac tcc atc aac 1123 Pro G u G y Ser Val I e Tyr Pro G y Val Val Ser His Ser lie Asn 330 335 340 gct gtg gag cac cca cgc ctg gtt gct gat cgt atc gtt cag ttc gcc 1171 Ala Val G u His Pro Arg Leu Val Ala Asp Arg Ile Val G n Phe Ala 345 350 355 aag ctt gtt ggc cct gag aac gtc att gcg tcc act gac tgt ggt ctg 1219 Lys Leu Val y Pro G u Asn Val Ile Ala Ser Thr Asp Cys y Leu 360 365 370 ggc gga cgt ctg cat tcc cag atc gca tgg gca aag ctg gag tcc cta 1267 G y G y Arg Leu His Ser G n lie Ala Trp Ala Lys Leu G u Ser Leu 375 380 385 gta gag ggc gct cgc att gca tca aag gaa ctg ttc taagctagac 1313 Val G u Gy Ala Arg I e Ala Ser Lys G u Leu Phe 390 395 400 aacgagggtt gct 1326 <210> 232 <211> 401 <212> PRT <213> Cor ynebact eri um glut am cum <400> 232 IVet Ser G n Asn Arg IIe Arg Thr Thr His Val G y Ser Leu Pro Arg 1 5 10 Thr Pro G u Leu Leu Asp Ala Asn Ile Lys Arg Ser Asn G y Gu lie 25 Page 299 seqI i st car r ect ed. t xt a y a u a u Gu Phe Phe G n Il e Leu Gn Ser 40 Ser Val Asp Asp Val I Ie Tyr Tyr Tr p Thr a31u Pr o 145 Thr As p As n A a a31n 225 a31u A a Hi s Ph e Ser 305 a31u Ser I Ie Thr Ar g Hi s Ph e Ser Ph e 115 Pr o Ph e ValI Ph e Ph e 1 95 Ser As p Ser As n cys 275 As p a31u As n Ser a31n 355 Cys ValI Thr Ar g a31u As p Ser a31y Le u 1 65 A a As p a31u Pr o Ly s 245 A a ay le A a Le u 325 As n A a Le u As p Ser 70 Le u A a Ar g a31y Pr o 150 Le u A a Thr Tyr As p 230 As p ValI Ser a31y Ser 310 Pr a A a Ly s a31y Le u a31y a31y ValI Ar g I Ie 135 li1e Ly s Le u As p Ly s 215 Le u Tyr Ly s Tr p a31u 295 Pr a a31u ValI Le u a31y 375 I Ie ValI Le u Ser 1 05 Ar g Thr Tyr G y Pr a 1 85 a u I Ie a u As p Le u 265 a y Le u Hi s Ser Hi s 345 a y Le u As p As p Thr 90 Thr A a a31y I Ie vb t 1 70 a31y ValI Thr A a Tr p 250 Pr a Pr a Ar g A a ValI 330 Pr a Pr a Hi s I Ie Ph e vb t Pr a Le u Ar g a31y 1 As n Ser ValI As p Tr p 235 li1e Ly s Hi s A a Hi s 315 li1e Ar g a31u Ser Le u a31y Thr a31y Ph e A a 140 a31n A a A a A a A a 220 As p a31y a31u ValI a31u 300 a31u Tyr Le u As n a31n 380 As n A a As p As n Ser 125 Ser a31u A a A a A a 205 a31y a31n Thr a31n Thr 285 ValI Tr p Pr a ValI ValI 365 li1e a31u Tr p Thr I Ie 110 a31u ValI a31u G y Ar g 1 cys Le u I Ie Ar g Thr 270 As p a31y Ar g a31y A a 350 li1e A a a31y Tr p As p a31u A a a31y Thr A a 1 Le u A a Thr As n I Ie 255 Ar g I Ie a31y ValI ValI 335 As p A a Tr p Lys Leu au Ser Leu Val au ay Ala Arg lieAla Ser Lys au Leu Page 300 seql i st cor r ect ed. t xt 395 385 Phe <210> 233 <211> 548 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA02648 <400> 233 gac gca ccg Asp Al a Pro 1 agc gtg aag Ser Val Lys aac agt gca Asn Ser Al a tgc tgg ggc Cys Trp y gac atc at t Asp lie lle gaa ggc gca G u G y Al a aac aag ctt Asn Lys Leu t cc atc aac Ser Ile Asn 115 cag ttc gcc G n Phe Al a 130 tgt ggt ct g Cys G y Leu 145 gag t cc ct a G u Ser Leu ttg Leu 5 t ac Tyr aag Lys tgg Tr p gag G u cct Pr o gaa G u gt g Val ctt Leu gga G y gag G u 165 gca gaa gca tgg Ala u Ala Trp gat cag Asp n 10 atc aac cca li e Asn Pro gac Asp ctt Leu gga G y 55 ct g Leu cac Hi s tct Ser cac Hi s ggc G y 135 ct g Leu gct Al a tgg Tr p cca Pr o 40 cca Pr o cgc Ar g gca Al a gtt Val cca Pr o 120 cct Pr o cat Hi s at c I e aag Lys cac Hi s gca Al a cac Hi s at c SIle 105 cgc Ar g gag G u t cc Ser ggt G y gaa G u gt c Val gag G u gag G u 90 t ac Tyr ct g Leu aac Asn cag G n aca Thr cag G n act Thr gt c Val 75 tgg Tr p cct Pr o gtt Val gt c Val at c SIle 155 cgc Ar g acc Thr gac Asp ggt G y cgt Ar g ggt G y gct Al a at t SIle 140 gca Al a at c SIle cgc Ar g at c SIle ggc G y gt a Val gtt Val gat Asp 125 gcg Al a tgg Tr p gat Asp ct g Leu cca Pr o ttc Phe tgg Tr p gt g Val 110 cgt Ar g t cc Ser gca Al a gag cca G u Pro gcc atc Ala I e cac atc His Il e ttc ggt Phe G y tcc ttc Ser Phe gaa gaa G u G u tct cac Ser His atc gtt Ile Val act gac Thr Asp aag ctg Lys Leu 160 ttc Phe 175 cgc att gca Ar g II e Al a 170 t ca aag gaa ct g Ser Lys G u Leu taagctagac aacgagggtt gct <210> 234 <211> 175 <212> PRT <213> Corynebacteri um gl utami cum Page 301 seqI i st car r ect ed. t xt As p Ser As n cys As p (31u As n Ser O31n cys 145 (31u O> 234 Al a Pro Asp Ly s Al a G31y I Ie Al a Le u As n 115 Al a Le u Le u Le u Tyr Ly s Tr p O31u Pr o O31u ValI Le u G31y Ala Ou Ala Trp As p Le u G31y Le u Hi s Ser Hi s G31y 135 Le u lIe Ly s Hi s Al a Hi s I Ie 1 05 Ar g O31u Ser Thr O31n Thr ValI 75 Tr p Pr o ValI ValI I Ie 1 55 Ar g Thr As p G31y Ar g G31y Al a I Ie 140 Al a I Ie Ar g li1e G31y ValI ValI As p 125 Al a Tr p On lieAsn Pro As p Le u Pr o Ph e Tr p ValI 110 Ar g Ser Al a O31u Pro Al a I I e Hi s I I e Phe 03 y Ser Phe O31u (1u Ser Hi s I e Val Thr As p Lys Leu 1 Val O31u 1 Gy Ala Arg lieAla Ser Lys Ou Leu Phe <210O> 235 <21 1> 784 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (784) <223> FRXA02658 <400> 235 at gaat aaaa t tccgggt gc agt gaccgt a gagttt gat a ct t tct t tcg actt t tagat ggt gaggt aa t ggat t t t ca at c I Ie g at As p tt c Ph e g ac As p ac c Th r aac As n at t I Ie ggt (31y c ac Hi s aag Lys5 c ag O31n g ac As p ggt O31y t ct Ser t ct Ser ct t Le u t cc Ser aac As n gt a ValI 45 aac As n tt g Le u ggt O31y g at As p gag (31u ccc cgt Pr o Ar g 15 gag att O31u I Ie gac gt g As p Val ggc gaa (31Y (1u Page acgcggt t ag agt cgaat ga at g agc cag aac cgc I'b t Ser O31n As n Ar g 1 acc cca gag cta ctt Thr Pr o O31u Leu Leu ggg gag gag gaa ttc O3 y 03 u 03 u 03 u Phe atc aag cgc cag gtt Ilie Lys Ar g O31n Val t ac ggc cac gt c acc Tyr (3y Hi s Val Thr seql i st cor r ect ed. t xt tcc ggt gca gtt gac ttc ggt gca tgg tgg Ser y Ala Val Asp Phe y Ala Trp Trp 75 ctg ggc gga ctg acc atg acc gat acc gac Leu G y G y Leu Thr IVet Thr Asp Thr Asp 95 gca gtg cgt tcc acc cct ggc aac atc gag Ala Val Arg Ser Thr Pro y Asn Ile G u 105 110 cgt cgc gac cgc gca ttg ttc agc gaa gca Arg Arg Asp Arg Ala Leu Phe Ser G u Ala 120 125 ggc atc ttc acc ggt cgc gct tct gtg ggc Gy Ile Phe Thr Gy Arg Ala Ser Val y 135 140 cct att acc tac att ggc cag gaa gaa act Pro lie Thr Tyr lie Gy Gn Gu Gu Thr 150 155 ctg aag aag ggc atg aac gca gcg gga gct Leu Lys Lys G y IVet Asn Al a Al a G y Al a 170 175 gca cta tcc cca gga tct gca gct cga ttg Ala Leu Ser Pro y Ser Ala Ala Arg Leu 185 190 act gat gaa gaa gtc gtc gca gca tgt gct Thr Asp u u Val Val Ala Ala Cys Ala 200 205 tac aag atc atc acc gat gca ggt ctg acc Tyr Lys lie lie Thr Asp Ala y Leu Thr 215 220 <210> 236 <211> 228 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 236 Iet Ser G n Asn Arg Ile Arg Thr Thr His 1 5 10 Thr Pro G u Leu Leu Asp Ala Asn Ile Lys G y G u G u G u Phe Phe n Ile Leu n 40 Ile Lys Arg n Val Asp Leu Gy Ile Asp Tyr G y His Val Thr Ser G y Ala Val Asp 70 Tyr Ser Phe Thr Arg Leu y y Leu Thr 90 Trp Ala Ser n G u Al a Val Arg Ser Thr 100 105 Pag aac tac tcc ttc acc Asn 80 cgt Ar g ct g Leu t ac Tyr aac Asn cag G n 160 acc Thr acc Thr gat Asp gtt Val Tyr Ser Phe tgg gca agc Trp Al a Ser acc agc ttc Thr Ser Phe 115 gag gat cca G u Asp Pr o 130 cca gag ttc Pro G u Phe 145 acg gat gtt Thr Asp Val gac ggc ttc Asp G y Phe aac aag ttc Asn Lys Phe 195 gcg ctt tcc Ala Leu Ser 210 cag ctc gac G n Leu Asp 225 Thr cag G n 100 tct Ser gt a Val acc Thr gat Asp gtt Val 180 t ac Tyr cag G n gca Al a Pr o G u Asp Sy Tr p Asp G u cgc Ar g gaa G u gat Asp tct Ser gga G y ctg Leu 165 gca Al a gac Asp gaa G u Val Arg Ser I le Phe bet Pro e 303 Ser Asn Val Asn Al a Asp Asn seql i st cor r ec Thr Ser Phe Ser Asp Arg Arg Asp Arg Ala Leu 115 120 G u Asp Pro Val Ser G y lie Phe Thr y Arg 130 135 Pro u Phe Thr y Pro Ile Thr Tyr Ile y 145 150 155 Thr Asp Val Asp Leu Leu Lys Lys G y IVet Asn 165 170 Asp y Phe Val Ala Al a Leu Ser Pro y Ser 180 185 Asn Lys Phe Tyr Asp Thr Asp G u G u Val Val 195 200 Ala Leu Ser n G u Tyr Lys Ile lie Thr Asp 210 215 G n Leu Asp Ala 225 <210> 237 <211> 408 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(385) <223> RXC02238 <400> 237 ggcgcttagc caaaacatag agcggtaggg tatgcttatc ctcttaacac tactgtccat atacttttga aaaggtgtca ted. txt Phe Ser 125 Al a Ser 140 G n u Al a Al a Al a Al a Al a Al a 205 Al a G y 220 Al a Gy Thr Al a 175 Leu Al a Thr cgat t gagca acct t t cccg gtg acc aac gtg agc aac Asn acc Thr gcc Al a t ac Tyr act Thr aat Asn cac acc Thr cct Pr o gt g Val cag G n cag G n ggt Gy gcc Al a at c SIle ttc Phe gat Asp ggc Gy ttg Leu acc Thr gat Asp gca Al a gag G u gaa G u 75 gac Asp aag Lys gaa G u gcc Al a gga Gy aag Lys cac Hi s gcc Al a ct g Leu aag Lys 45 gt a Val cca Pr o gag G u gt c Val ct g Leu cgt Ar g ttc Phe ctt Leu gaa G u ttc Phe 15 gat Asp gcg Al a gag G u tct Ser ggt Gy Val Thr Asn Val Ser 1 gat ccg cca gtg ggc att Asp Pro Pro Val Gy Ile aag gtc act tcc aag tac Lys Val Thr Ser Lys Tyr cgc cag atc aac agc ttc Arg n lie Asn Ser Phe ttc atc gga cca ttg gtt Phe lie Gy Pro Leu Val att gct ctg cgt gag atc Ile Ala Leu Arg G u ile 80 taaaagacct tataact t ca Page 304 seql i st cor r ect ed. t xt <210> 238 <211> <212> PRT <213> Corynebact eri um gl ut arri cum <400> 238 Val Thr Asn Val Ser Asn G u Thr Asn 1 5 Pro Pro Val Gy Ile Thr Ala Pro Pro Val Thr Ser Lys Tyr Al a Leu Val lie 40 Sn lie Asn Ser Phe Tyr His n Ala 55 lie y Pro Leu Val Thr Pro G n Pro 70 Ala Leu Arg u lie Asn Ala y Leu Al a 10 SIle Phe Asp Gy Leu 90 Thr Asp Al a G u G u Asp Lys G u Al a Gy Lys Hi s Al a Leu Lys Val Pr o G u Phe Asp Al a G u Ser Gy <210> 239 <211> 1827 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1804) <223> RXC00128 <400> 239 ccattttccg tttggtcttg cctaaagaac cgcatggaaa cgttgatcgc tccagagaca ccgtgggaag gggagcagca acg Thr gct Al a t ca Ser aat Asn t at Tyr acg Thr ct g Leu tgt Cys t cc Ser gat Asp act Thr agc Ser gcc Al a acg Thr t cc Ser gat Asp cag G n tgg Tr p ct c Leu ctt Leu caa G n tt g Leu t at Tyr 75 aat Asn acc Thr ccg Pr o agc Ser ttg Leu gaa G u ccg Pr o gcg Al a cag G n aca Thr 45 at c SIle gcg Al a gct Al a gt g Val aac Asn caa G n cgc Ar g gcg Al a gcg Al a ct g t ct Leu Ser 15 acg gat Thr Asp gag at a G u Ile ggc ttc G y Phe aag gcg Lys Al a 80 t cg act Ser Thr 95 Page 305 ttatcgtgaa gcaccgatcc gtg agt aaa att tcg Val Ser Lys Ile Ser 1 gtg acc act ctg gtg Val Thr Thr Leu Val ccg caa gtg ctg cgc Pro G n Val Leu Arg gca ggg ccg acc ccg Ala y Pro Thr Pro ttc agc gca ggt gcg Phe Ser Al a y Al a tat ctg acg gaa ggg Tyr Leu Thr G u y cgt att ttg gat cgc Arg lie Leu Asp Arg 100 seqi i st car r ect ed. t xt at t gat ct g aac act ct g cca ggt t cg I Ie Asp Leu Asn Thr Leu Pro G31y Ser acg aat gcg gaa Th r As n A a 0 u cga acg att 1 05 gcg A a c ag O31n g at As p 1 50 ag a Ar g g at As p gag (31u cct Pr o gcg A a 230 gga G31y t gg Tr p g ac As p ct c Le u tt t Ph e 310 agt Ser at t I Ie gag (31u cgt Ar g 120 gag (31u gag (31u g at As p t ct Ser c ag (31n 200 ccg Pr o tt c Ph e tt g Le u tt g Le u gcg A a 280 g aa (31u tt g Le u ct a Le u t cc Ser gt g ValI 360 ac g Thr gcg A a cgt Ar g cgg Ar g 1 70 c ag O31n at g vb t at t I Ie ggg G31y g at As p 250 cat Hi s tt g Le u aac As n g at As p ggc G31y 330 gcc A a act Thr cag (31n gag O31u at c I e 155 aac As n gt g ValI t cc Ser t ct Ser t t c Ph e 235 g at As p gct A a ct g Le u cca Pr o gga G31y 315 at t I e aat As n gt t ValI gt c ValI tt t Ph e 140 g at As p cat Hi s tt g Le u ac g Thr cct Pr o 220 aat As n gcg A a g at As p t cg Ser gag O31u 300 t cg Ser t gg Tr p gt g ValI ggc G31y gga (31y 125 g aa O31u gct A a t ac Tyr gt g ValI gt g ValI 205 ggt G31y g at As p cgt Ar g gt c ValI gag O31u 285 gct A a tt g Le u ag c Ser gt g ValI t cc Ser 365 110 ac g Thr gct A a tt g Le u act Thr ggg G31y 1 90 ct g Le u gt g ValI ggg G31y Le u gca A a 270 tt c Ph e t ac Tyr t cg Ser ggt G31y gca A a 350 at g vb t tt g Le u gag O31u ccg Pr a ccg Pr a 1 75 g at As p at g vb t gt c ValI gag O31u cgt Ar g 255 ggc G31y cca Pr a ac c Thr ag g Ar g gga G31y 335 gcg A a g aa O31u ct c Le u at c I Ie g ac As p 1 60 c ac Hi s cgg Ar g gcc A a aat As n t at Tyr 240 tt c Ph e ccc Pr a ac g Thr aac As n gt c ValI 320 g at As p gt a ValI ggc G31y ggt (31y 130 at g vb t at t I Ie gt g ValI t gg Tr p ct g Le u 210 ct g Le u tt c Ph e gcc A a act Thr ac c Thr 290 gt g ValI t cc Ser g at As p c ac Hi s act Thr 370 Ar g 115 ggc (31y cgt Ar g tt a Le u t at Tyr tt g Le u 1 gt t ValI t cc Ser act Thr c ag O31n tt g Le u 275 ac c Thr t cc Ser ggc G31y t ct Ser g aa O31u 355 t ca Ser Thr I Ie gt g ValI cgg Ar g tt a Le u tt c Ph e 1 tt c Ph e aat As n ac g Thr ggt G31y gcc A a 260 gt c ValI g at As p ac g Thr aat As n gca A a 340 aac As n g at As p t at Tyr g aa O31u gag O31u 1 tt t Ph e aat As n ggt (31y g at As p tt g Le u 245 gt g ValI gct A a g ac As p tt g Le u gt g ValI 325 gcg A a aac As n gcg A a 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 Page 306 seql i st cor r ect ed. t xt ttg agg agt gaa acg atc act Leu Arg Ser G u Thr I e Thr 375 380 ggg Gy 390 tcg Ser cca Pr o ggg Gy gt a Val gt g Val 470 gac Asp cgc Ar g ct c Leu gt c Val at t SIle 550 gtt Val tgg Tr p aca Thr gat Asp cgg Ar g 440 cgt Ar g ccg Pr o at t SIle gag G u gcg Al a 520 gat Asp cgc Ar g t ac Tyr gt g Val ggt Gy 410 acg Thr gcc Al a ggt Gy ttg Leu ctt Leu 490 gac Asp gt g Val cat Hi s gt g Val gt g gat Val Asp 395 gag ct c G u Leu ggt ccg G y Pro at g at c Vet Ile ccg ggc Pro y 460 ggc gag Gy Gu 475 gtg ggc Val G y gga tcg G y Ser gtg gcg Val Ala gcg atg Al a Vbet 540 ccc ggt Pro G y cgt ccc acc ttt Arg Pro Thr Phe ggg gag acg cct Gy u Thr Pro 400 gtc cag acg gag Val G n Thr G u 415 atc tct gaa ttc Ile Ser G u Phe 430 att gaa ggc aag ile G u G y Lys 445 gag cgg cgc gtg G u Arg Arg Val gcg gcg ctg tcg Al a Al a Leu Ser 480 acg tca att cca Thr Ser Ile Pro 495 gcg att tcg tcg Ala lie Ser Ser 510 gtg gca agt tcc Val Al a Ser Ser 525 ctt cag ctg ccg Leu G n Leu Pro ttg ctg ggc acg Leu Leu G y Thr gaa G u 385 gt c Val gcg Al a caa G n gt g Val aca Thr 465 at c SIle gag G u at g Met gcg Al a act Thr 545 cga Ar g gag G u ct g Leu t ac Tyr 450 aat Asn aac Asn acg Thr ccg Pr o acg Thr 530 gcc Al a gca Al a gt g Val 420 cga Ar g ggc G y acg Thr cgc Ar g ct g Leu 500 ggg Sy gt c Val aat Asn tac gcg tcg agt Tyr Ala Ser Ser cga Ar g 405 ct g Leu act Thr gt c Val gag G u cca Pr o 485 tgg Tr p aat Asn t ac Tyr gat Asp gt g Val 565 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1827 cgt gcg gcg ccg Arg Ala Ala Pro 560 tgatggagct gttcttcccg cgc <210> 240 <211> 568 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 240 Val Ser Lys I e Ser Thr Lys Leu Lys Ala Leu Thr Ala Val Leu Ser 1 5 10 Val Thr Thr Leu Val Al a y Cys Ser Thr Leu Pro G n Asn Thr Asp 25 Pro G n Val Leu Arg Ser Phe Ser y Ser n Ser Thr On u Ile 40 Page 307 Al a 03 y Pro Th r Pro Asn O31n As 55 seqi i st car r ect ed. t xt p Pro Asp Leu Leu 11 e Arg G31y Ph e Ph e Tyr Ar g Al a Ser Thr 145 G31y As p Ar g Le u O31n 225 O31n Al a Tyr Le u Thr 305 Ser I Ie Ar g ValI (31u 385 ValI Al a Thr Le u Ar g 115 G31y Ar g Le u Tyr Le u 1 95 ValI Ser Thr O31n Le u 275 Thr Ser O31y Ser O31u 355 Ser Al a ValI Al a O31y Ar g I Ie Tyr O31u O31u 1 65 Ph e As n O31y As p Le u 245 ValI Al a As p Le u ValI 325 Al a As n Al a Ser Ar g 405 Pr o Ar g As p I Ie Pr o 135 O31y As n Pr o Ser Ser 215 Ser As n Thr O31y Al a 295 Al a Pr o Ser Al a Ar g 375 Le u Al a O31n Thr As n 1 05 O31y As n Tr p Le u O31y 1 85 Ser Al a ValI As p Al a 265 Pr a Tyr O31n O31n Ser 345 Le u O31u Al a Thr Tyr As n Le u O31n O31u I Ie 1 As n ValI Ser Ser Ph e 235 As p Al a Le u Pr a O31y 315 li1e As n ValI I Ie ValI 395 O31u Pr a Pr a ValI Ph e 140 As p Hi s Le u Thr Pr a 220 As n Al a As p Ser O31u 300 Ser Tr p ValI O31y Thr 380 As p Al a Al a O31y O31y 125 O31u Al a Tyr ValI ValI 205 O31y As p Ar g ValI O31u 285 Al a Le u Ser ValI Ser 365 Ar g O31y Al a Al a Ser 110 Thr Al a Le u Thr O31y 1 Le u ValI O31y Le u Al a 270 Ph e Tyr Ser O31y Al a 350 vb t Pr a O31u Ser Thr Le u O31u Pr a Pr a 1 As p vb t ValI O31u Ar g 255 O31y Pr a Thr Ar g O31y 335 Al a O31u Th r Th r (3y (3u Leu Val (31n Thr (31u 410 415 Page 308 Al a G n Val Thr 465 I le G u bet Al a Thr 545 Ar g l Ie Ser 435 Val SIle Tr p Pr o Ser 515 Thr Asp Al a Leu Thr Val G u Pr o 485 Tr p Asn Tyr Asp Val 565 Ar g Val Thr 455 Al a Gy Val Ser Thr 535 Tr p Al a seql i st cor r ect ed. t xt Asp Val Thr Gy Pro lie 425 Arg Ala Ala IVet Ile Ile 440 445 Arg Pro y Pro Gy u 460 Pro Ser Leu Gy G u Al a 475 Ile Leu Leu Val y Thr 490 G u G n Asp G y Ser Al a 505 Ala Pro Val Val Ala Val 520 525 Asp Ser His Ala IVet Leu 540 Arg G u Val Pro G y Leu 555 Tyr Ser 430 G u Ar g Al a Ser l Ie 510 Al a G n Leu G u Gy Ar g Leu SIe 495 Ser Ser Leu Gy <210> 241 <211> 1344 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1321) <223> RXA02240 <400> 241 cagctagacc actgacattg cagttttaga cagcttggtc aagactattt attctcaact tcttcgaaag aagggtattt gcc Al a aaa Lys aaa Lys at c SIle cct Pr o cgt Ar g tgt Cys ccg Pr o cat Hi s tta Leu ttc Phe gct Al a tcg Ser gtt Val cgc Ar g agt Ser t cc Ser gt c Val gag G u aag Lys gaa G u gat Asp gca Al a 45 gt c Val ct c Leu tct Ser acc Thr gt g Val cgt Ar g at c SIle gt a act Val Thr 15 att ttg I e Leu gaa act G u Thr acc agc Thr Ser gaa at c G u lie 80 Page 309 tatattggtt ttttgtattt gtg gct cag cca acc Val Ala G n Pro Thr 1 gag gga cat cca gac Su G y His Pro Asp gac gcg ctg ctc gaa Asp Ala Leu Leu G u gtg gtc acc acc gga Val Val Thr Thr G y gct tac gta gag atc Ala Tyr Val u Ile gga ttc aac tcc tct G y Phe Asn Ser Ser seqI i st car r ect ed. t xt gag gt t gga t tc gac gga O31u Val G31y Phe Asp G31y cgc acc t gt Ar g Thr Cys gag O31u cgc Ar g c ag O31n cct Pr o 1 50 gt t ValI c ag O31n ac c Thr t tg Le u gca A a 230 cct Pr o ac c Thr ggt O31y gct A a ct t Le u 310 aag Ly s ct g Le u t cc Ser aac As n 120 ct g Le u cct Pr o aaa Ly s ac c Thr gt c ValI 200 ac c Thr at t I Ie ggt O31y cgc Ar g gga O31y 280 t ac Tyr g at As p gt c ValI g ac As p g aa O31u g ac As p tt c Ph e gcg A a ggc O31y 1 70 gca A a t cc Ser ct g Le u g at As p tt c Ph e 250 at c I Ie tt c Ph e at g vb t gct A a ctt Le u 330 c ag (31n at c I Ie gt t ValI ggc O31y t t g Le u 155 at c I Ie t ac Tyr acc Thr cgc Ar g ct g Le u 235 at t I Ie at c I Ie t cc Ser cgt Ar g gaa O31u 315 t ac Tyr at t I Ie gct A a g aa O31u t ac Tyr 140 gcg A a gt t ValI g at As p c ag O31n g aa (31u 220 gca A a ct g Le u gt g ValI ggt G31y t gg Tr p 300 gt t ValI gt t ValI c ag (31n g ac As p g aa (31u 125 gcc A a c ac Hi s cct Pr o gcg A a c ac Hi s 205 c ac Hi s ac c Thr ggt G31y g at As p aag Ly s 285 gt a ValI c ag O31n g aa O31u gct A a ggc O31y 110 g ac As p ac c Thr cga Ar g c ac Hi s c aa O31n 1 90 g ac As p gt c ValI ggt O31y ggc O31y ac c Thr 270 g at As p gca A a gt t ValI ac c Thr gcc A a 350 ggc gt c t ca gt a t cc O3 y Val Ser Val Ser 95 gt g gat aac t cc gac Val As p As n Ser As p 115 gac cgc gca ggt gct Asp Ar g Ala O31y Ala 130 aac gaa acc gaa gag Asn 03 u Thr 03 u 03 u 145 ct g t ca cgt cgt ct g Leu Ser Arg Arg Leu 1 60 ct g cgt cca gac gga Leu Ar g Pr o Asp O31y 1 75 gac cgc cct agc cac Asp Ar g Pr o Ser Hi s 1 cca gaa gt t gac cgt Pro O31u Val Asp Ar g 210 at t gat t gg gt a at c Ilie Asp Tr p Val Ilie 225 gag at c acc gt g tt g O31u Ilie Thr Val Leu 240 ccc at g ggt gat gcg Pr o 'b t O31y Asp Ala 255 t ac ggt ggc at g gct Tyr 03 y 03 y I'b t A a 275 cca agc aag gt g gac Pr o Ser Lys Val Asp 290 aag aac atc gtg gca Lys Asn Ilie Val Ala 305 gca tac gcc at t gga A a Tyr A a Il e 03 y 320 ttt gac acc aac aag Phe Asp Thr Asn Lys 335 gt g t tg gag gt c tt t Val Leu (3 u Val Phe 355 Page 310 at c I Ie 1 00 g aa O31u O31y t ac Tyr ac c Thr aaa Lys5 1 ct g Le u gca A a aaa Lys5 at c I Ie ggt O31y 260 cgc Ar g cgc Ar g gca A a cgc Ar g g aa O31u 340 g ac As p ggt O31y gcc A a g ac As p at g vb t c ag O31n 1 ac c Thr g at As p t gg Tr p g ac As p aac As n 245 ct g Le u cat Hi s t ct Ser ggc O31y gca A a 325 ggc O31y ct g Le u 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 seqI i st car r ect ed. t xt cgt cca gca gca at t at c cgt gag ct t gat ct g ct t cgt Ar g Pr o Al a Al a I Ie I Ie Ar g O31u Leu Asp Leu Leu Ar g 360 365 370 gct gac act gct gcc t ac ggc cac t t t ggt cgc act gat Al a Asp Thr Al a Al a Tyr G31y Hi s Phe G31y Ar g Thr Asp 375 380 385 cct t gg gag gct at c gac cgc gt t gat gaa ct t cgc gca Pr o Tr p O31u Al a Ilie Asp Ar g Val Asp O31u Leu Ar g Al a 390 395 400 t tg gcc t aaaaat ct g at gt agt at c tt c Leu Al a ccg at c t ac Pr o I Ie Ty r t tg gac ct t Leu Asp Leu gcc ct C aag Al a Leu Lys 405 1219 1267 1315 1344 <210O> <211 <21 2> <21 3> 242 407 PmT Cor ynebact er i urn gi ut anri cumn <400> 242 ValI O31u As p ValI Al a G31y Ser As n Al a Th r 145 Ar g Pr a Pr a ValI Tr p 225 Ala O1n Pro Thr Ala Hi s Le u Th r ValI As n Ser As p 115 Al a O31u Le u G31y Hi s 1 95 Ar g As p O31u G31y I Ie Ser O31y Al a As p Nibt O31n 1 65 Th r As p Tr p ValI I Ie As p ValI O31n ValI O31n Th r O31y 135 Le u Ar g ValI ValI O31u 215 Le u As p O31n ValI ValI Ph e O31n 1 O31y vb t I Ie O31u Ph e 1 li1e O31n Ph e 10 Al a Ser ValI Ar g As p 90 O31u As p Ph e Al a O31y 1 70 Al a Ser Le u Th r I Ie Ar g O31y As n O31y I Ie ValI O31y Le u 1 li1e Ty r Th r Ar g O31u As p Al a ValI Le u Th r As p O31u 125 Al a Hi s Pr a Al a Hi s 205 Hi s ValI lie O31u Th r O31u O31y ValI As p As n Le u Le u 1 As p Pr a I Ie Val Ilie Lys AspAiac3y IleOu Asp Leu AaThr Oy Ou Ilie 230 235 240 Page 311 Thr Gy Gy Lys SIle 305 Al a Thr G u Leu Thr 385 Ar g Leu Al a Al a 275 Asp Al a Gy Lys Phe 355 Pr o Leu Al a Asn 245 Leu Hi s Ser Gy Al a 325 Gy Leu Tyr Leu Lys 405 Pr o Thr Gy Al a Leu 310 Lys Leu Ar g Al a Pr o 390 Leu Ser Gy Gy Al a 295 Al a Pr o Ser Pr o Asp 375 Tr p Al a seql i st cor r ect ed. t xt Sy Ser Phe lie Leu Gy 250 Arg Lys Ile Ile Val Asp 265 G y Al a Phe Ser G y Lys 280 285 Tyr Ala IVet Arg Trp Val 300 Asp Arg Ala G u Val G n 315 Val y Leu Tyr Val G u 330 Asp Gu Gn lie Gn Ala 345 Ala Ala lie Ile Arg G u 360 365 Thr Al a Al a Tyr G y Hi s 380 Su Ala lie Asp Arg Val 395 Sy Thr 270 Asp Al a Val Thr Al a 350 Leu Phe Asp Pr o 255 Tyr Pr o Lys Al a Phe 335 Val Asp Gy G u <210> 243 <211> 669 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(646) <223> RXA00780 <400> 243 ccttttaagt ccttctttgc ccgtgaataa ttctctggat tcacgctgtt agacttgcct gcatgctctc gacaataaaa ct c Leu aac Asn gcc Al a gcc Al a aac Asn gt g Val agc Ser ttc Phe cgt Ar g t ac Tyr tgg Tr p cga Ar g gaa G u t cc Ser aaa Lys tt c Phe cac Hi s gga G y t cc Ser ct c Leu cca Pr o cac Hi s ttc Phe ggc G y gca gcc Al a Al a 15 gcc at c Ala I e cgc ggc Arg G y att gaa Ile G u Page 312 agtttccacg tgcagt t aag atg atc cgt gaa gat IVet Ile Arg u Asp 1 cga ggc gat tta gaa Arg y Asp Leu G u tgg gca cat cga gtt Trp Ala His Arg Val ccc gcc cgc gta tta Pro Ala Arg Val Leu att cac ccc ggt gcc lie His Pro G y Ala seql i st cor r ect ed. t xt acc att ggt cgt cgc ttt ttt att gac cac gga atg gga atc gtc atc 355 Thr I I e y Arg Arg Phe Phe lie Asp His G y Met y I I e Val lie 75 80 ggc gaa acc gct gaa atc ggc gaa ggc gtc atg ctc tac cac ggc gtc 403 G y G u Thr Ala G u IIe G y G u G y Val Mt Leu Tyr His G y Val 95 100 acc ctc ggc gga cag gtt ctc acc caa acc aag cgc cac ccc acg ctc 451 Thr Leu Gy Gy G n Val Leu Thr G n Thr Lys Arg His Pro Thr Leu 105 110 115 tgc gac aac gtg aca gtc ggc gcg ggc gca aaa atc tta ggt ccc atc 499 Cys Asp Asn Val Thr Val y Ala y Ala Lys Ile Leu y Pro lie 120 125 130 acc atc ggc gaa ggc tcc gca att ggc gcc aat gca gtt gtc acc aaa 547 Thr lie Gy u y Ser Ala lie Gy Ala Asn Ala Val Val Thr Lys 135 140 145 gac gtg ccg gca gaa cac atc gca gtc gga att cct gcg gta gca cgc 595 Asp Val Pro Ala G u His IIe Ala Val G y IIe Pro Ala Val Ala Arg 150 155 160 165 cca cgt ggc aag aca gag aag atc aag ctc gtc gat ccg gac tat tac 643 Pro Arg G y Lys Thr G u Lys Ile Lys Leu Val Asp Pro Asp Tyr Tyr 170 175 180 att taagaacagt tagcgcccta cct 669 Ile <210> 244 <211> 182 <212> PRT <213> Corynebacter i um gl ut am cum <400> 244 IVet Ile Arg u Asp Leu Ala Asn Ala Arg u His Asp Pro Ala Ala 1 5 10 Arg G y Asp Leu G u Asn Ala Val Val Tyr Ser Gy Leu His Ala lie 25 Trp Ala His Arg Val Al a Asn Ser Trp Trp Lys Ser G y Phe Arg G y 40 Pro Ala Arg Val Leu Al a G n Phe Thr Arg Phe Leu Thr Gy lie G u 55 lie His Pro G y Ala Thr lie G y Arg Arg Phe Phe lie Asp His G y 70 75 'et Gy Ile Val Ile Gy Gu Thr Ala Gu Ile Gy Gu Gy Val bet 90 Leu Tyr His G y Val Thr Leu G y G y G n Val Leu Thr G n Thr Lys 100 105 110 Arg His Pro Thr Leu Cys Asp Asn Val Thr Val G y Ala G y Ala Lys 115 120 125 Ile Leu Gy Pro Ile Thr Ile Gy u y Ser Ala lle Gy Ala Asn 130 135 140 Ala Val Val Thr Lys Asp Val Pro Al a G u Hi s I e Al a Val G y I e 145 150 155 160 Page 313 seqI i st car r ect ed. t xt Pro Ala Val Ala Arg Pro Arg Gy Lys Thr Ou Lys Ilie Lys Leu Val 165 170 175 Asp Pro Asp Tyr Tyr I Ie 1 <210O> 245 <21 1> 1056 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1033) <223> RXA00779 <400> 245 cagaggccac t tcaagt aga t gt t tcgt aa t tgt t tacag caacaaaaac agcacttcaa tgattggagc accacccgac cgtttacgca agcggtcgac at g ggc aat gt g t ac 11! I'bt Gy Asn Val Tyr aac As n aag Ly s tt c Ph e g aa (31u g aa (31u gca A a gag (31u cca Pr a gt c ValI gca A a 1 50 g ac As p at c I Ie ac c Thr cca Pr a gca A a ac c Thr gga G31y cgc Ar g gca A a 120 g aa (31u cca Pr a g ac As p g aa (31u ggc G31y aac As n aag Ly s ggc G31y aac As n ct c Le u ggc G31y g aa (31u gt c ValI ac c Thr 1 70 at c I Ie g ac As p gt c ValI ggt G31y ac c Thr gt t ValI cgc Ar g c ag O31n gca A a 140 cgc Ar g g at As p ggc G31y gca A a aag Ly s 45 gca A a ggt G31y ct c Le u gct A a ggt G31y 125 gt c ValI g ac As p at c I Ie c ac Hi s act Thr g ac As p ct g Le u at c I Ie ac c Thr t ac Tyr 110 gca A a ct t Le u ac c Thr tt c Ph e ac c Thr 15 gt c ValI cgt Ar g aag Ly s gca A a at g vb t 95 ggt G31y aag Ly s gct A a ac c Thr gt t ValI 1 75 cca Pr a ct g Le u at c I Ie cca Pr a ct g Le u ccg Pr a gca A a g ac As p cgc Ar g gcg A a 1 gca A a 1 ct g Le u gt c ValI ggt G31y G31y gca A a gag G31u gag O31u aag Ly s c ag O31n 145 aag Ly s ag c Ser gt a ValI aag Ly s ct g Le u ggc G31y at g vb t ac c Thr at c I Ie gca A a 130 tt c Ph e g aa O31u tt c Ph e ct g Le u gag O31u at c I Ie at c G31y t cc Ser 1 00 ct t Le u g aa O31u aac As n ct c Le u ac c Th r 1 Page 314 seqI i st car r ect ed. t xt gga G31y gt a ValI ggC G31y ccc Pr o 230 aac As n at c I Ie ct t Le u ac c Thr gt c acc Val Thr 1 85 gt c t ac Val Ty r 200 gct ggt Al a G3 y gt c ct g Val Leu gac gca As p Al a ggc ggt G31y (1y 265 gca aag Al a Lys 280 ttc ggc Phe G3 y ggc gt t ggc cag 31y Va 1(3 Gy 1n acc gt c Thr Val cca cac Pr o Hi s gac cgc As p Ar g 235 atc gca I e Al a 250 at c t cc I I e Ser cca gag Pr o G3 u gag cgc G31u Ar g gag G31u aag Lys5 220 aag Lys5 tt c Ph e ac C Thr aac As n t ac Tyr 300 cca Pr o 205 at c I Ie gt t ValI t cc Ser ggc G31y gct Al a 285 gt c ValI gtc CCtg aag gaa Val Leu Lys G3 u 1 90 gaa gcg t cc cca a31u Al a Ser Pr o cag ggc at c ggc a31n a31y Ilie 31y 225 ct C gac gac gt g Leu Asp Asp Val 240 cgc aag ct C gct Arg Lys Leu Ala 255 gca aac atc aag Al a Asn Il e Lys 270 ggc aaa acc at c a31y Lys Thr Ilie t cc acc gt t ct t Ser Thr Val Leu 305 aac aac gca Asn Asn Al a 1 ct t ct g acc Leu Leu Thr 210 gca aac ttc Al a Asn Phe ct g acc gt c Leu Thr Val acc gaa gag Thr a3 u a3 u 260 gca gct ctt Al a Al a Leu 275 gt c acc gt t Val Thr Val 290 tac gaa gac Tyr a3 u Asp 691 739 787 835 883 931 979 1 027 1 056 cgc gac t aat tct tag cgact gt taa cca Ar g Asp 310 <210O> 246 <21 1> 311 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 246 I'b t a3 y Asn Val Tyr Asn Asn I I e Thr 1 5 Leu Val Lys Leu Asn Lys Leu Thr a3 u Val Lys Leu a31u Ser Phe Asn Pro Al a 40 a31y Leu Al a I Ie Val a31u As p Al a a31u a31y a31y Thr I Ie Val a31u Al a Th r Ser 70 Al a I'bt Val a31y Al a Al a Ar g a31y Ty r a31u Thr I'b t Ser As n a31u Ar g Ar g Val 100 105 a31u I Ie Val Leu Thr Pr o a31y Al a Al a 115 120 a3 u Th rI 10 a3 y Leu As n Ser Lys Ser a31yAs n As n Val 90 Leu Leu a31y Ibt Page 315 a31y Al a Lys5 Al a a31y Le u Al a a31y 125 Thr ValI Ar g Al a vb t a31y seql i st cor r ect ed. t xt Lys Ala Asp Gu Ile Val Ala Ou Arg Ou Asn Ala Val 130 135 140 Leu Al a Arg G n 145 Lys Ser G u Pr o Gy 225 Val Al a Lys SIle Leu 305 Su I e Gy Asn 195 Leu Asn Thr G u Al a 275 Thr G u G u G u 165 Gy Asp Al a SIle Ser 245 Gy Asp Val SIle Al a 150 Asp Gy Val Gy Pr o 230 Asn SIle Leu Thr Ar g 310 Asn Thr Thr G n Lys 215 G u G u Leu Al a Asp 295 Asp Pr o Asp Val Val 200 Al a Val Asp Gy Al a 280 Phe Ar g Gy Thr 185 Tyr Gy Leu Al a Gy 265 Lys Gy Asn 155 Val Val Val Hi s Ar g 235 Al a Ser G u Ar g Asp SIle G n Pr o 205 I le Val Ser Gy Al a 285 Val Thr Phe Val 190 G u G n Leu Ar g Al a 270 Gy Ser Thr Val 175 Leu Al a Gy Asp Lys 255 Asn Lys Thr <210> 247 <211> 623 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> RXN00402 <400> 247 act gac gaa aag gat gga aag cca gta Thr Asp G u Lys Asp G y Lys Pro Val 1 5 gat gct gct tac cac gga ttg aag tac Asp Ala Ala Tyr His G y Leu Lys Tyr 25 ttc ggc ctc aag gtt cgc gtt ggc ctt Phe y Leu Lys Val Arg Val y Leu 40 ctc tcc gca ttc aac gca tgg gct gca Leu Ser Ala Phe Asn Ala Trp Ala Ala tcc ctg cgc ctg gag cgc cac aac gaa Ser Leu Arg Leu G u Arg His Asn G u 70 ttg ccc 1 Leu Pro 10 gca gac Al a Asp I ct a cgc Leu Arg gtc cag Val G n aac gcc Asn Ala I Page 316 ttc Phe ggt Gy acc Thr at c SIle aag Lys gt c Val gca Al a ggc Gy gac Asp gtt Val act Thr cca Pr o t cc Ser acc Thr gca Al a ttc Phe aag Lys acc Thr tgg Tr p ggc Gy 145 cag G n gt c Val ct c Leu aac Asn t cc Ser t cc Ser 115 ttt Phe gtt Val gac Asp ct g Leu ggc Gy 195 cac Hi s tgg Tr p ct c Leu gac Asp t cc Ser gct Al a 165 gtt Val ttt Phe gag G u tac Tyr acc Thr gcc Al a ct c Leu 150 ggc Gy ggc Gy gct Al a aag Lys gca Al a ttc Phe ct g Leu 135 gtt Val ct g Leu at c SIle gca Al a seql i st cor r ect ed. t xt gtg gaa aag gtt aac ttc gca ggc Val G u Lys Val Asn Phe Ala y 90 acc aag gaa aag ctt ggc ctg aag Thr Lys G u Lys Leu G y Leu Lys 105 110 gag atc aag ggc ggc aag gat gag G u Ile Lys G y G y Lys Asp G u 120 125 aag cta cac tcc aac ctt gca aac Lys Leu His Ser Asn Leu Ala Asn 140 gtt cac cca gca acc acc acc cat Val His Pro Ala Thr Thr Thr His 155 gca cgc gcg ggc gtt acc cag tcc Ala Arg Ala y Val Thr G n Ser 170 175 gag acc att gat gat atc atc gct G u Thr Ile Asp Asp Ile Ile Ala 185 190 atc tagctttaaa tagactcacc cca Ile 200 <210> 248 <211> 200 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 248 Thr Asp G u Lys Asp G y Lys Pro 1 5 Asp Ala Ala Tyr His G y Leu Lys T Phe y Leu Lys Val Arg Val y L 40 Leu Ser Ala Phe Asn Ala Trp Ala P 55 Ser Leu Arg Leu G u Arg His Asn C 70 Phe Leu Asn Asn His G u Lys Val C Lys Asp Ser Pro Trp Tyr Ala Thr L 100 1 Thr y Ser Val Leu Thr Phe G u I 115 120 Trp Ala Phe Ile Asp Ala Leu Lys L 130 135 G y Asp Val Arg Ser Leu Val Val F 145 150 (al Leu Pro Tyr Phe Val Thr Pro yr 25 .eu l a Su Su ys 05 le .eu i s 10 Al a Asp Leu Leu Arg Asp Val n y Asn Ala lle Lys Val Asn 90 G u Lys Leu Lys G y G y Hi s Ser Asn 140 Pro Al a Thr 155 Page 317 Gy Thr I le Lys Phe Gy Lys 125 Leu Thr Al a Gy Asp Val Al a Leu 110 Asp Al a Thr Pr o Ser Thr Al a Gy Lys G u Asn Hi s seql i st cor r ect ed. t xt Sn Ser Asp u Ala G y Leu Ala Arg Ala y Val Thr n Ser Thr 165 170 175 Val Arg Leu Ser Val Gy Ile u Thr Ile Asp Asp lie lie Ala Asp 180 185 190 Leu G u G y G y Phe Al a Al a lie 195 200 <210> 249 <211> 599 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA00402 <400> 249 gta ttg ccc Val Leu Pro 1 tac gca gac Tyr Al a Asp ctt ct a cgc Leu Leu Arg gca gtc cag Ala Val n gaa aac gcc G u Asn Ala gaa aag gtt G u Lys Val aag gaa aag Lys G u Lys atc aag ggc Ile Lys G y 115 ct a cac t cc Leu Hi s Ser 130 cac cca gca Hi s Pr o Al a 145 cgc gcg ggc Ar g Al a G y acc att gat Thr lie Asp ttc Phe 5 ggt Gy acc Thr at c SIle aag Lys ttc Phe ggc Gy aag Lys ctt Leu acc Thr acc Thr 165 at c SIle gtc act cca gat gct gct tac cac gga ttg aag Val Thr Pro Asp Ala Ala Tyr His G y Leu Lys 10 gca Al a ggc Gy gac Asp gtt Val 70 gca Al a ct g Leu gat Asp gca Al a acc Thr 150 cag G n at c SIle cca Pr o t cc Ser acc Thr 55 gca Al a ggc G y aag Lys gag G u aac Asn 135 cat Hi s t cc Ser gct Al a gcc Al a acc Thr 40 ctt Leu gaa G u ct g Leu t ac Tyr gct Al a 120 at c SIle t ca Ser acc Thr gac Asp ttc Phe ct c Leu t cc Ser ttc Phe aag Lys acc Thr 105 tgg Tr p ggc Sy cag G n gt c Val ct c Leu ct c Leu gca Al a cgc Ar g aac Asn 75 t cc Ser t cc Ser ttt Phe gtt Val gac Asp 155 ct g Leu aag Lys ttc Phe ct g Leu aac Asn cct Pr o gtt Val at c SIle cgc Ar g 140 gaa G u t cc Ser gtt Val aac Asn gag G u cac Hi s tgg Tr p ct c Leu gac Asp 125 t cc Ser gct Al a gtt Val cgc Ar g gca Al a cgc Ar g gag G u t ac Tyr acc Thr 110 gcc Al a ct c Leu ggc Sy ggc Gy gtt Val tgg Tr p cac Hi s aag Lys gca Al a ttc Phe ct g Leu gtt Val ct g Leu at c SIle 175 ggc G y gct Al a aac Asn gtg Val acc Thr gag G u aag Lys gtt Val gca Al a 160 gag G u gaa ggc ggc ttt gct gca atc Su y y Phe Ala Ala lle Page 318 seql i st cor r ect ed. t xt 180 185 tagct t t aaa tagactcacc cca <210> 250 <211> 192 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 250 Val Leu Pro Tyr Phe Val Thr Pro Asp 1 Tyr Leu Al a G u G u Lys SIle Leu Hi s 145 Ar g Thr Asp Ar g G n Al a Val Lys Gy 115 Ser Al a Gy Asp 5 Gy Thr SIle Lys Phe Gy Lys Leu Thr Thr 165 I le Al a Gy Asp Val 70 Al a Leu Asp Al a Thr 150 G n I I e Pr o Ser Thr Al a Gy Lys G u Asn 135 Hi s Ser Al a Al a Thr 40 Leu G u Leu Tyr Al a 120 I le Ser Thr Asp Phe Leu Ser Phe Lys Thr 105 Tr p Gy G n Val Leu 185 Al a 10 Gy Ser Leu Leu Asp 90 Gy Al a Asp Ser Ar g 170 G u Al a Leu Al a Ar g Asn Ser Ser Phe Val Asp 155 Leu Gy Hi s Val Asn G u Hi s Tr p Leu Asp 125 Ser Al a Val Phe Gy Ar g Al a Ar g G u Tyr Thr 110 Al a Leu Gy Gy Al a 190 Leu Val Tr p Hi s Lys Al a Phe Leu Val Leu SIe 175 Al a <210> 251 <211> 613 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(613) <223> RXS00405 <400> 251 agaataaatt tataccacac agtctat t gc aatagaccaa gctgt t cagt agggtgcatg ggagaagaat ttcctaataa aaactcttaa ggacctccaa atg cca aag tac gac 115 IVet Pro Lys Tyr Asp 1 Page 319 seql i st cor r ect ed. t xt c ttt aaa acc cac tc aat t cc aat gct Asn Ser Asn Al a gac Asp cag tgg gg Sn Trp G c att cac y Phe G u Thr Arg Ser IIe His 10 ggc Gy caa G n ttc Phe acc Thr cac Hi s ttg Leu t ac Tyr at c SIle gca Al a 150 aac Asn t ca Ser acc Thr ctt Leu gag G u gt a Val ct g Leu ggc Gy 120 gtt Val gtt Val cag G n gac Asp ttc Phe gat Asp ttg Leu ttc Phe gga Gy gag G u ttc Phe cca Pr o gac Asp 170 gca Al a gt g Val ct a Leu gaa G u 75 t cc Ser gcg Al a act Thr gt g Val aac Asn 155 gt c Val cag G n ttc Phe ggc Gy aac Asn t cc Ser ggc Gy ct a Leu gaa G u 140 acc Thr acc Thr gac Asp 45 cct Pr o cgc Ar g gga Gy gac Asp ttc Phe 125 aac Asn aaa Lys agc Ser t cc Ser gtt Val at c SIle cag G n cac Hi s 110 ctt Leu ccc Pr o gca Al a gca Al a gct Al a t ac Tyr gct Al a gcc Al a 95 at c SIle at c SIle gac Asp ttc Phe cga Ar g gag G u t cc Ser t cc Ser 80 gca Al a gt c Val act Thr gac Asp ttc Phe 160 ctt Leu gcc Al a ct c Leu gaa G u acc Thr t cc Ser aac Asn 130 gag G u gag G u ccg Pr o aag Lys acc Thr ggt G y aac Asn cca Pr o 115 cgc Ar g t cc Ser act Thr at c SIle cag G n aac Asn ggc G y gcc Al a 100 cgc Ar g ct g Leu tgg Tr p ttc Phe gca Al a t ac Tyr cgt Ar g cca Pr o gtc Val att SIe ct c Leu ggt Sy cag G n gcc Al a 165 <210> 252 <211> 171 <212> PRT <213> Corynebact <400> 252 IVet Pro Lys Tyr 1 Arg Ser Ile His Asn Leu Pro lie His Ala Lys G n Arg Leu Thr Asn Leu G u G y G y er i um gl ut am cum Asp Asn Ser Asn Ala 5 Al a y n Ser Val Tyr G n Ser Thr Al a 40 Arg Phe Ala Leu G u Pro Thr Val G u Ala 70 Val His Ala Val Ala Asp n 10 Asp Al a Phe Val I Asp Leu Leu G u Phe Ser 90 Page 320 Sy Thr Asp Pr o Ar g Gy G u Al a Al a Tyr Al a Al a seqI i st car r ect ed. t xt Thr Thr Asn Al a Il e Leu Asn Leu Al a G y Al a G y Asp Hi s I e Val 100 105 110 Thr Ser Pr o Ar g Leu Tyr G31y G31y Thr O31u Th r Leu Phe Leu I Ie Thr 115 120 125 Leu As n Ar g Leu G31y I Ie As p Val Ser Phe Val (31u As n Pr o Asp Asp 130 135 140 Pro (31u Ser Tr p O31n Al a Al a Val O31n Pr o As n Thr Lys Al a Phe Phe 145 150 155 160 G31y O31u Thr Phe Al a As n Pr o O31n Al a As p Val 165 170 <210O> 253 <21 1> 1812 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (1789) <223> RXCO0164 <400> 253 ct gct t tgcg ggaggt tat g aaat gagt gg ggagacgt cg aaaagcat gc gct t tccgt t ggccagcct g ccgcaagt gc ggcgcgaggt g gcccg g cag ggt cgt at t G31y Ar g I Ie cgg Ar g gct Al a ct g Le u at t I Ie tt c Ph e g aa O31u g at As p gag O31u ct g Le u aag Ly s gcg Al a t cc Ser at t I Ie gt g ValI at g vb t ggc G31y t cc Ser 120 ac c Thr t gg Tr p gt g ValI G31y gt g ValI t cg Ser ggc G31y ggc G31y gcg Al a gcc Al a tt t Ph e ct g Le u gcg Al a gca Al a cgg Ar g 75 acc Thr g at As p gt g ValI gcg Al a tt a Le u gt g ValI c ag O31n at t I Ie at t I Ie gcg Al a tt g Le u ac a Thr ac g Thr ggc G31y ccg Pr a at g vb t 45 gcc Al a t ct Ser ct t Le u gt g ValI gag O31u 125 at c I Ie gcg ct g Al a Leu 15 cag gt g 03 n Val cgt gat Ar g As p ggc gcg (3 y Al a gag aag a3 u Lys ggg t tg 03 y Leu 95 agc cgc Ser Ar g 110 acc gt c Thr Val at t gcg I e Al a gt g ValI ct g Le u tt t Ph e gt g ValI at t I Ie ccc Pr a t cc Ser ccg Pr a ct g Le u cgg Ar g gag O31u agt Ser gcc Al a c ac Hi s g at As p tt a Le u 130 agt Ser at t lIe ct c Le u gcg Al a aat As n c ag O31n g at As p 115 agt Ser gcg Al a gt g ValI agt Ser t gc cys tt g Le u gt g ValI 1 00 gt c ValI t cc Se r ct g t tt t ct t tg gac t gg Leu Phe Ser Leu Asp Tr p Page 321 seql i st cor r ect ed. t xt caa G n 150 t cc Ser gcg Al a cgt Ar g at t SIle acc Thr 230 at g Met gcg Al a ct g Leu t cc Ser ccg Pr o 310 gaa G u gac Asp gca Al a cgg Ar g cac Hi s 390 gag G u gt g Val cac Hi s at g let act Thr 200 cag G n at g let gcg Al a acg Thr ggc Sy 280 t at Tyr cct Pr o cgc Ar g gac Asp ggc Sy 360 cca Pr o tct Ser cat Hi s at t SIle ttg Leu 170 gag G u cgg Ar g tcg Ser at t SIle gcg Al a 250 ggc Sy at g Met tcg Ser ccc Pr o gt c Val 330 acg Thr ggt Gy caa G n cgc Ar g ttc Phe gt c Val aag Lys gcg Al a t at Tyr tct Ser 220 aac Asn gt g Val gtt Val at g Met gcg Al a 300 agc Ser ttt Phe aat Asn acg Thr caa G n 380 cgt Ar g ggc G y gt g Val gcg Al a cga Ar g t cc Ser 205 gt g Val at g Met at c SIle acc Thr ttc Phe 285 cgc Ar g ggt G y agc Ser t cc Ser acg Thr 365 gt g Val at c SIle acg Thr gt g Val ccg Pr o aag Lys 190 at g let gt g Val tgg Tr p ggc G y ggt G y 270 at g Met at c SIle gt g Val t at Tyr ggc Sy 350 gt c Val ctt Leu gcc Al a ct g Leu gcg Al a gat Asp 175 gt a Val gaa G u gt c Val at g Met t ac Tyr 255 gcc Al a cgc Ar g gt g Val aaa Lys ggc Sy 335 gaa G u gcc Al a gt c Val cgc Ar g ccg Pr o 160 cgg Ar g ctt Leu gat Asp aag Lys ct g Leu 240 aag Lys gt g Val gt g Val gga G y gca Al a 320 gat Asp act Thr gcc Al a gac Asp ttg Leu 400 145 gt g Val t at Tyr gag G u gcc Al a ggt Sy 225 ttt Phe ct g Leu ct g Leu ct c Leu gtt Val 305 cct Pr o t cc Ser gt c Val ttg Leu gac Asp 385 gcc Al a t ac Tyr gcg Al a gct Al a at g let 210 at t SIle gcg Al a gt c Val at g Met gac Asp 290 gtt Val cag G n tgg Tr p gcg Al a ct g Leu 370 ttc Phe at g let t ac Tyr gca Al a at t l Ie 195 cat Hi s cgt Ar g gaa G u at t SIle at t SI e 275 acc Thr gcg Al a ggc G y gcg Al a ct c Leu 355 gcg Al a ccc Pr o gt c Val ttc Phe gaa G u 180 cgc Ar g aat Asn gcg Al a ttc Phe gat Asp 260 at t SIe at t SIe gat Asp aaa Lys gt g Val 340 gt g Val ggc Sy gt c Val agc Ser 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 cgc cag gat Arg G n Asp Page 322 ctc acc ttg gct Leu Thr Leu Ala seql i st cor r ect ed. t xt aaa cca gat Lys Pro Asp aat gcc ct t Asn Ala Leu 440 gtt ggt gcg Val Gy Al a 455 gcg ttg gcc Al a Leu Al a 470 gaa gcc acg G u Al a Thr gct gca gat Al a Al a Asp cgg ttg gat Arg Leu Asp 520 ggg gag gtt G y G u Val 535 ggt att tat G y Ile Tyr 550 410 t cc Ser tgg Tr p gga Sy gt g Val gaa G u 490 gt g Val gca Al a gaa ctu cgt gat Asp tt g Leu at c SIle tt g Leu 475 gca Al a agc Ser tcg Ser t cc Ser ct g gag G u gag a u cag G n 460 ttg Leu gga Sy aag Lys cgg Ar g ggt G y 540 tgg Tr p gaa G u agt Ser 445 ct a Leu ct c Leu tcg Ser aac Asn gct Al a 525 act Thr tta Leu 430 ctt Leu gaa G u aat Asn gcg Al a cgt Ar g 510 gat Asp cac Hi s 415 gcg Al a cca Pr o cca Pr o ccg Pr o ggt Sy 495 t cc Ser cag a n cag G n cat Hi s gaa G u gt g Val gcg Al a 480 gcc Al a gca Al a at t SIle gag G u gct Al a gga Sy gt g Val 465 at c l Ie agc Ser ttg Leu ct g Leu tta Leu 545 ctt Leu ct g Leu 450 gct Al a gt c Val gca Al a gt g Val gt g Val 530 ttg Leu caa G n acg Thr cag G n at g Met gaa G u 500 gcg Al a gat Asp cac Hi s 1411 1459 1507 1555 1603 1651 1699 1747 1789 1812 Sn Arg Leu 555 act gcg tgg Thr Al a Trp agt gtc gga aga Ser Val Gy Arg 560 tagttgactg ttcaatgcgt tga <210> 254 <211> 563 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 254 Val y Arg Ile Pro Arg Ala Lys Trp 1 5 Leu Leu Ser Ala y Al a Tyr Al a Ser 25 Sy Arg lie Val Asp Leu Val Ser Asp 40 Val G u Leu Ser Val Ile Leu lie Ala 55 Leu Ser Al a Cys G y Phe Tyr Val Val 70 lie Ala Asn Leu Arg G u Asp IVet Val Thr His G n Val G u Asp Ala G y Ser 100 105 Trp Phe Leu 10 Val Leu Val G y Al a n Val Ala lle Ser Arg Il e G y Thr Al a 90 G y Asp Leu Page 323 Sy Pr o Met Al a Ser Leu Val Al a G n Ar g Sy G u Sy Ser 110 Leu Val Asp Al a Lys Leu Ar g seqi i st car r ect ed. t xt rAl a Al a ValI Th r O31u Thr Asp Asp 115 Val Ser 03 u Leu Se 120 I Ie Ph e 145 ValI Tyr O31u Al a G31y 225 Ph e Le u Le u Le u ValI 305 Pr o Ser ValI Le u As p 385 Al a As p Al a G31y Ser Le u Tyr Al a I Ie 1 95 Hi s Ar g Ou le le 275 Thr Al a G31y Al a Le u 355 Al a Pr o ValI Thr G31y 435 As p Ser Tr p Al a 1 65 Ar g G31y O31n Ar g Le u 245 As n Ar g O31n Pr o ValI 325 Ly s G31y Le u Ser O31n 405 Al a ValI ValI Ph e 135 Ph e Ly s Al a Al a As p 215 Thr Le u Le u Ar g G31y 295 li1e Le u I Ie Ser ValI 375 Le u ValI Pr o Al a G31y 455 Thr ValI Hi s vb t Thr 200 O31n vb t Al a Thr O31y 280 Tyr Pr a Ar g As p O31y 360 Pr a Ser Hi s As p Le u 440 Al a I Ie Le u Tyr Al a 1 ValI Al a Le u ValI I Ie 265 Pr a Al a ValI As n I Ie 345 Al a As p As p ValI Al a 425 As p Ar g Al a I Ie Le u 1 70 O31u Ar g Ser I Ie Al a 250 O31y vb t Ser Pr a ValI 330 Thr O31y O31n Ar g Ph e 410 Ser Tr p O31y Al a Pr a 1 55 Ser Ar g Al a Tr p Le u 235 Le u Al a As n Le u As p 315 Ser I Ie Ly s O31y O31u 395 Ser As p Le u I Ie 125 li1e ValI Al a Ar g Ser 205 ValI vb t I Ie Thr Ph e 285 Ar g O31y Ser Ser Thr 365 ValI I Ie Thr O31u Ser 445 Le u Thr Val Pr o Al a Al a As p 1 ValI O31u ValI vb t Tyr 255 Al a Ar g ValI Ly s O31y 335 O31u Al a ValI Ar g Ar g 415 Al a Pr a Pr a Le u Pr a 1 Ar g Le u As p Ly s Le u 240 Ly s ValI ValI O31y Al a 320 As p Thr Al a As p Le u 400 O31n Hi s O31u ValI Val Ala O1n On Leu Ala Leu Ala Arg Val 465 470 Leu Leu Leu Asn Pro Ala Page 324 I Ie Ser Le u Le u Le u 545 ValI seqI i st car r ect ed. t xt I I e I'b t Asp G u Al aThr Al a G3 u Al a G3 y Se r Al a (3 y Al a 485 490 495 Leu (31u (31u Al a Al a Asp Al a Val Ser Lys Asn Ar g Ser Al a 500 505 510 Val Al aHi sAr g Leu Asp (1n Al aSer Ar gAl aAsp (1n Ilie 515 520 525 I'b t Asp Lys a31y a31u Val Val a31u Ser a31y Thr Hi s 31n a31u 535 540 Asp His a31y a31y Ilie Tyr a31n Ar g Leu Tr p Thr Al a Tr p Ser 550 555 560 Ar g <210O> 255 <21 1> 1713 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1690) <223> RXCO1191 <400> 255 cgct gct t tc acgcaact ga aaccgcaccg gat caagtt a cgt gt tggt g gccgt cgcgg ggt tggt agg gccct gggcg t t tggggt tg t tct t tgtgg gt g ggt gga ct c gt c 11! Val (31y a31y Leu Val g at As p ct g Le u ggC a31y cgc Ar g g aa a31u Cgg Ar g gcg Al a t gg Tr p ct c Le u at c I Ie gcg Al a g at As p gcg Al a gt g ValI tt t Ph e ct c Le u 120 gca Al a gct Al a at g vb t tt g Le u cgc Ar g act Thr gt g ValI ct t Le u acc Th r ggc a31y gcg Al a cgc Ar g ggc a31y 75 gcg Al a gt g ValI gt c ValI ccg Pr o a31y cgc Ar g gcg Al a g ac As p gcg Al a at t I Ie ggt (31y ag c Ser gt t ValI gcg Al a 45 gcg Al a gt g ValI ag c Ser t cc Ser tt g Le u 125 at g vb t gt t ValI tt g Le u gt g ValI at t I Ie ac c Th r gcg Al a 110 gt c ValI g ac As p ag c Ser ccg Pr o tt g Le u cgt Ar g gt g ValI ggc a31y at c I Ie 1 gt t ValI ct g Le u gcg Al a g at As p at c I Ie ccg Pr o at g vb t ccg Pr o gt a ValI ggc a31y at c lIe gcg Al a gcg Al a ct g Le u gcg Al a ct g Le u 130 gt g ValI ac g Th r gcg Al a aac As n g at As p at g Nibt gcg Al a 115 t at Ty r tt c Ph e t gg Tr p ggg a31y ac g Th r g at As p gt g ValI 1 00 gt t ValI t gg Tr p Page 325 seql i st cor r ect ed. t xt acg ttg cgc gtc Thr Leu Arg Val 135 tat tta ccc cgc tca Tyr Leu Pro Arg Ser -0 .0 cgc Ar g 150 ggc G y cgt Ar g ttc Phe at c SIle gat Asp 230 cga Ar g caa G n gca Al a tta Leu aag Lys 310 ggt Sy ct g Leu tct Ser caa G n gca Al a 390 gcc Al a gaa G u gac Asp ttc Phe 200 ct c Leu gt c Val ttc Phe gcc Al a aac Asn 280 ctt Leu gca Al a acc Thr agc Ser gt c Val 360 at c SIle ccc Pr o G y ttg Leu 170 gcc Al a aca Thr ct c Leu gt c Val cca Pr o 250 gca Al a gt c Val gac Asp gt g Val gct Al a 330 act Thr ccg Pr o tgc Cys gcc Al a gt g Val 155 cgc Ar g t cc Ser tgg Tr p at c SIle ggc Gy 235 at c SIle tcg Ser agc Ser gt c Val cag G n 315 ggt G y t cc Ser gaa G u tt c Phe acc Thr 395 cgc Ar g gct Al a ggc G y gca Al a ttg Leu 220 gca Al a ggc Gy ct g Leu ggc Gy t cc Ser 300 ct g Leu aaa Lys ggg G y gca Al a cga Ar g 380 gat Asp acg Thr ttc Phe gaa G u ttt Phe 205 ggc G y gt c Val acg Thr at c SIle acc Thr 285 cac Hi s gaa G u agc Ser cag G n ttg Leu 365 gga G y gcg Al a cag G n cgc Ar g gcc Al a 190 t cc Ser acc Thr t ca Ser ct c Leu cgc Ar g 270 tcg Ser cac Hi s cca Pr o acg Thr gt g Val 350 cgc Ar g tct Ser gac Asp ggt ccg ctt tat acg Sy Pro Leu Tyr Thr 145 cgg ctt gtc ggc gca Arg Leu Val G y Al a 160 gca gaa gat aca gaa Al a G u Asp Thr G u 175 cgc gac att tcc att Arg Asp Ile Ser Ile 195 cgc aac aac cgc gcg Arg Asn Asn Arg Ala 210 ggc ttt tac ctg gtc G y Phe Tyr Leu Val 225 acc gcc gca ctg atc Thr Ala Ala Leu Ile 240 gtg ggc atg ttc tcc Val G y IVet Phe Ser 255 atg gtg ggc gtt att Met Val y Val lie 275 ccg gcg tct gcc agc Pro Ala Ser Ala Ser 290 tat cac act gca ccc Tyr His Thr Ala Pro 305 ggg gaa cac atc gcc G y G u Hi s I Ie Al a 320 ctc gcc ctc att gcg Leu Ala Leu Ile Ala 335 gct ctc ggc gga tcg Ala Leu G y G y Ser 355 cag aag atc gcg atg G n Lys Ile Ala IVet 370 gtt tta gat aat ctt Val Leu Asp Asn Leu 385 atc cac gcc gtt ctc Ile His Ala Val Leu 400 Page 326 cgt Ar g gt c Val tta Leu 180 tct Ser gaa G u aac Asn ttc Phe gac Asp 260 aac Asn acc Thr gt c Val at t SIe gca Al a 340 agt Ser gt c Val cgt Ar g gcc Al a 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 seql i st cor r ect ed. t xt at t SIle gt g Val gcg Al a gaa Gu gca Al a 470 cgc Ar g gga gcy ggc Gy gat Asp gat Asp gct Al a 440 acg Thr ctt Leu aac Asn at c SIle t at Tyr 520 tgg ttg gag Trp Leu G u 410 gct ttc cgt Al a Phe Ar g gta cat ttg Val Hi s Leu gaa tca ggc G u Ser G y 460 gt c act gaa Val Thr G u 475 gcg aaa acc Ala Lys Thr 490 gaa t ct gga Su Ser G y caa ct g t gg G n Leu Trp cgc tta ccg Arg Leu Pro 415 tta acc tct Leu Thr Ser 430 gcc gac ct a Al a Asp Leu 445 t ct gat cat Ser Asp Hi s aac aga tca Asn Arg Ser gcc gat cgc Al a Asp Ar g 495 acc cat gaa Thr His G u 510 act gcg tgg Thr Al a Trp 525 caa ggc G n y gtg gaa Val G u gca at c Ala I e gca aaa Al a Lys 465 gcc at c Ala I e 480 at c at c Ile Ile gag ctt G u Leu tct gcg Ser Al a ata gac acc atc Ile Asp Thr Ile 420 aac cag atc atg Asn G n Ile IVet 435 gtc atc ctt gat Val lie Leu Asp 450 cag ctt gaa gat G n Leu G u Asp atc gtg gct cac Ile Val Ala His 485 gtc atg gac t cc Val IVet Asp Ser 500 cga gcg atc ggc Arg Ala lie y 515 cgc taattagcca Ar g 530 1363 1411 1459 1507 1555 1603 1651 1700 1713 cccaagacca cgc <210> 256 <211> 530 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 256 Val y y Leu Val Asp Lys Leu Leu 1 5 Val Val Val Phe Ala Leu Leu Ile Val 25 Leu y Thr Trp Trp G y Ser Ala Leu 40 Ala lie Ala y Leu Arg u Asp Val 55 Asp Ala Asn Thr I e u Thr Al a y 70 lie Ala Asp Asp Ser Arg G u Val Ser Pro Leu IVet Val G n Al a y Phe Thr 100 105 Iet Al a Al a Val Asp Trp Arg Leu G y 115 120 Pro Leu Tyr Trp Thr Thr Leu Arg Val 130 135 Al a Thr Pro 10 Al a G y G y IVet Al a Arg Leu Arg Ala Arg G y Asp Thr Al a Al a 90 Val Val I e Leu Val G y Tyr Leu Pro 140 Page 327 Ser Val Al a Al a Val Ser Ser Leu 125 Ar g Mbet Val Leu Val SIle Thr Al a 110 Val Ser Ar g Ser G u Ser Ser Val Phe Al a Gy seql i st cor r ect ed. t xt Leu Tyr Thr Arg G u Arg 145 150 G u Al a Phe G y Val Arg Thr G n Arg Val Asp SIle Asn Tyr 225 Al a bet Gy Ser Thr 305 Hi s Leu Gy SIle Asp 385 Al a Gy G u SIle Lys 465 I le Al a Su I e 195 Al a Val l Ie Ser SIle 275 Ser Pr o Al a Al a Ser 355 vbt Leu Leu Asp G n 435 I le Leu Val G u 165 Lys Val Cys SIle Hi s 245 I le Al a Al a SIle Val 325 Gy Phe Ser SIle Asp 405 I le bet Asp Asp Hi s 485 Gy Ar g Phe SIle Asp 230 Ar g G n Al a Leu Lys 310 Gy Leu Ser G n Al a 390 I le Val Al a G u Al a 470 Ar g Al a SIle Ar g Thr 215 Leu Leu Ser Ser Thr 295 Asn Al a Leu Asn G u 375 Ar g Gy Gy Leu Al a 455 Al a Leu Thr Al a 185 Leu Val Thr Gy Ser 265 G n Phe Ser Gy Pr o 345 G u Hi s G u Ser Gy 425 Ar g Al a Lys G n Leu 170 Al a Thr Leu Val Pr o 250 Al a Val Asp Val Al a 330 Thr Pr o Cys Al a Tr p 410 Al a Val G u Val Al a 490 155 Ar g Ser Tr p SIle Gy 235 I le Ser Ser Val G n 315 Sy Ser G u Phe Thr 395 Leu Phe Hi s Ser Thr 475 Lys Al a Sy Al a Leu 220 Al a Sy Leu Gy Ser 300 Leu Lys Sy Al a Ar g 380 Asp G u Ar g Leu Sy 460 G u Thr Phe G u Phe 205 Sy Val Thr SIle Thr 285 Hi s G u Ser G n Leu 365 Sy Al a Ar g Leu Al a 445 Ser Asn Al a Ar g Al a 190 Ser Thr Ser Leu Ar g 270 Ser Hi s Pr o Thr Val 350 Ar g Ser Asp Leu Thr 430 Asp Asp Ar g Asp Al a 175 Ar g Ar g Sy Thr Val 255 bet Pr o Tyr Sy Leu 335 Al a G n Val SIle Pr o 415 Ser Leu Hi s Ser Ar g 495 Leu 160 G u Asp Asn Phe Al a 240 Sy Val Al a Hi s G u 320 Al a Leu Lys Leu Hi s 400 G n Val Al a Al a Al a 480 I e IIe Val Met Asp Ser O y u IIe IIe Gu Ser y Thr His O u u Page 328 seql i st cor r ect ed. t xt 500 505 510 Leu Arg Ala lie Gy G y Arg Tyr G u n Leu Trp Thr Ala Trp Ser 515 520 525 Al a Ar g 530 <210> 257 <211> 1392 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1369) <223> RXA02646 <400> 257 ttggaaataa aacct at gcc aaagt aggt g caat t ctagg agaagat t ac act agt caac catgagtgaa acatacgtgt ctgagaaaag tccaggagt g atg gct agc gga gcg let Al a Ser G y Al a gag G u t cc Ser gaa G u cgt Ar g caa G n gcc Al a at c SIle gtt Val gac Asp acg Thr 150 acc Thr at t SIle at t SIle gga Gy t ac Tyr cag G n ggc Gy gtt Val ggc Gy 120 gca Al a at c SIle gct Al a gcc Al a cca Pr o gaa G u at c SIle gat Asp gcc Al a gt g Val gag G u gct Al a cct Pr o gag G u gcc Al a act Thr at c SIle cgc Ar g gca Al a 75 t at Tyr cag G n ttt Phe gca Al a tt c Phe 155 at c SIle gac Asp cca Pr o t ac Tyr ggt G y ggt G y gt g Val act Thr gt c Val gcg Al a 140 gat Asp ttg Leu at t SIle ttg Leu ctt Leu 45 gcg Al a at c SIle tgc Cys cca Pr o t cc Ser 125 cat Hi s gct Al a tcg Ser caa acg gcg G n Thr Al a 15 cag tat tgc G n Tyr Cys aag cgt gag Lys Arg G u ct g aac t ct Leu Asn Ser gtt gcc gca Val Al a Al a 80 aag tcc ttg Lys Ser Leu 95 aag caa aag Lys G n Lys 110 ttg gtg gtc Leu Val Val gaa gat gca G u Asp Al a cgc aac acc Arg Asn Thr 160 cag ct g act G n Leu Thr gca Al a cgt Ar g ct g Leu gcg Al a gca Al a gtt Val gac Asp ggc Sy 130 cgc Ar g at c SIle cga Ar g ctt Leu cag G n cag G n ggt G y cag G n cgc Ar g 115 aat Asn acc Thr ggt Sy at t I e tct Ser gat Asp ct c Leu aac Asn gga Sy 100 at c SIle aac Asn ggc Sy cag G n t cc Ser gag G u gtt Val acc Thr cat Hi s cgc Ar g at g Met ttc Phe gca Al a ggc Sy 165 t cc atg ggc aag agt Ser let G y Lys Ser Page 329 seql i st cor r ect ed. t xt gca Al a gt g Val at c SIle gga Gy 230 gt c Val ggt Gy at g let gcg Al a gt c Val 310 gcg Al a ttc Phe gaa G u aag Lys agt Ser 390 gca Al a cac Hi s agc Ser 200 cca Pr o at c SIle cgt Ar g gt g Val gat Asp 280 tct Ser gt g Val at c l Ie gt g Val at c SIle 360 aac Asn gca Al a gat Asp at g Met at g let gga Gy ttg Leu gga Gy 250 at g let t ac Tyr gct Al a at c SIle gag G u 330 ttc Phe gga Gy cgt Ar g gga Gy cgt Ar g 410 gtt Val gct Al a gca Al a gag G u 235 gat Asp at g Met caa G n ggg Gy at c SIle 315 cgc Ar g ccg Pr o ccg Pr o gag G u tt g Leu 395 cgc Ar g cca Pr o gat Asp gca Al a 220 act Thr ct c Leu agc Ser aac Asn ttg Leu 300 tct Ser t cc Ser caa G n gat Asp acc Thr 380 gat Asp ct c Leu gt c Val at g let 205 t cc Ser gtt Val aac Asn gcg Al a gaa G u 285 aag Lys ggt Gy ttg Leu aag Lys gat Asp 365 ggt Gy tct Ser gag G u ggc Gy 190 gca Al a at g let gat Asp t ac Tyr acc Thr 270 ggc Gy gaa G u ggc Gy gt g Val cct Pr o 350 gac Asp act Thr ttg Leu ccg Pr o ggc Gy cgc Ar g gct Al a ttt Phe 240 at c SIle ggc Gy at c SIle t cc Ser aac Asn 320 cgc Ar g cag G n acg Thr ttg Leu gaa G u 400 acc Thr gga Gy act Thr gca Al a 225 gt g Val gt g Val gct Al a gcg Al a ttt Phe 305 gat Asp ggt Gy ttg Leu ct g Leu gt g Val 385 cgt Ar g cct Pr o ctt Leu gcg Al a 210 ttg Leu gac Asp gag G u gt g Val gag G u 290 gca Al a gt g Val tta Leu cgt Ar g ttt Phe 370 ggt Gy at g let gag G u gca Al a gtt Val aat Asn gca Al a aac Asn 260 act Thr gct Al a cgc Ar g cgt Ar g cac Hi s 340 ttc Phe t ac Tyr cac Hi s gaa G u gaa G u 420 ggt Gy ggt Gy ggt Gy gca Al a 245 cag G n gag G u ggc G y t ct Ser t at Tyr 325 t ac Tyr ct g Leu ct c Leu ttg Leu tcg Ser 405 t ac Tyr 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1392 ttg acc Leu Thr taaacatagc tgaaggccac ct c <210> 258 Page 330 seqI i st car r ect ed. t xt <21 1> 423 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 258 N/t Al a Ser (31y Al a G31u O31n Pr a As p O31y Ser O31y Ar g Thr O31u 145 ValI Ser O31y Thr Al a 225 ValI ValI Al a Al a Ph e 305 As p Ar g Le u O31n O31n G31y O31n Ar g 115 As n Thr O31y O31y Le u 1 95 li1e Hi s O31y Ly s cys 275 Pr a Al a Le u Ser O31u ValI Thr Hi s Ar g vb t Ph e Al a O31y 1 65 Ser O31y O31y O31y Al a 245 O31n O31u O31y Ser Tyr 325 Ser O31u Ar g O31n 70 Al a I Ie ValI As p Thr 150 Thr Al a ValI I Ie O31y 230 ValI O31y vb t Al a ValI 310 Al a Le u ValI Thr Ser O31u O31n Tyr Hi s O31u 135 Le u ValI As p ValI O31u 215 Pr a Ly s Ar g Le u Le u 295 ValI O31u le le Oy 40 Tyr O31n O31y ValI O31y 120 Al a I Ie Al a Hi s Ser 200 Pr a I Ie Ar g ValI As p 280 Ser ValI I Ie Ar g Al a Al a Ly s Ar g ValI Pr a 1 05 O31y Ser O31u Al a ValI 1 85 Tyr Al a Thr ValI Hi s 265 Le u I Ie cys Al a Al a 10 Pr a Ou le As p Al a 90 ValI O31u Al a Pr a O31u 1 70 vb t vb t O31y Le u O31y 250 vb t Tyr Al a I Ie O31u 330 Al aAsp Il e (On Thr Pr o Leu O31n li1e Tyr Leu Lys Ar g O31y Al a Leu Al a 03 y I I e Val 75 Ty r Val Cys Lys O31n Thr Pr o Lys 110 Phe Val Ser Leu 125 Al a Al a Hi s 03 u 140 Phe Asp Ala Arg 1 55 Il e Leu Ser 03 n Val Pr o Val O31y 1 Al a Asp I'b t Al a 205 Al a Al a Ser I'b t 220 O31u Thr Val Asp 235 Asp Leu Asn Tyr I'b t Ser Al a Thr 270 31n Asn (1u (1y 285 O3 y Leu Lys 03 u 300 Il e Ser 03 y 03 y 315 Ar g Ser Leu Val Th r Al a Ty r Cys Ar g O31u Asn Ser Al a Al a Ser Leu O3 n Lys Val Val As p Al a Asn Thr 1 Leu Thr 1 O31y (1y Pr o Ar g O3 n Al a Pr o Phe 240 Thr I Ie 255 Ou y le le I'b t Ser As n As n 320 Ar g Ar g 335 Page 331 G31y Le u Le u ValI 385 Ar g Pr o Ly s Hi s 355 (31u I Ie (31u Tyr Tyr Le u Le u Le u Ser 405 Tyr Ph e (31u Ly s Ser 390 A a Le u Le u As p Ar g 375 (31u I Ie Thr seqI i st car r ect ed. t xt Val Asn Phe Pro On Lys Pro G1y On 345 350 li1e Leu G31y Pro As p As p Asp I Ie Thr 360 365 As n As n Ar g O31u Thr G31y Thr Ala Leu 380 Ala Ser G31y Leu As p Ser Leu Leu (31u 395 400 As p Ser Ar g Ar g Leu (31u Pr o G31y Thr 410 415 <210O> 259 <21 1> 966 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (943) <223> RXA00766 <400> 259 tatggcgctt gaaccacaaa taaagtctgc accgacgccg ct acggcggg t ccat ccggc aacaaaaccc caacct acca g at As p cgc Ar g gca A a g aa O31u gca A a ac g Th r aaa Ly s gga (31y gcc A a gga G31y gca A a gcc A a gag O31u ct g Le u gcg A a t cc Ser 120 ac c Th r gcc A a ct a Le u c aa O31n t ca Ser t ca Ser cgt Ar g g ac As p cga Ar g t gc Cy s t t g Le u at g vb t t gc Cy s 75 gga G31y gaa (31u acc Th r ggc G31y aac As n gga G31y G31y ac c Th r t gg Tr p c ac Hi s ggc (31y ggc G31y cgc Ar g cca Pr o g aa (31u ac g Th r ac c Th r gt a ValI 110 ccc Pr o at c lIe 15 cga Ar g gag (31u t cc Ser ct c Le u at c I Ie 95 t cg Ser gga (31y tt t Ph e c ac Hi s ccg Pr a t gg Tr p ag c Ser 80 ac c Th r gt c ValI at c I Ie gt gat ct taa t tgt cgaacc I'b t Val Phe Tr p As p 1 gaa aca ct c ct c at c 1& (31u Thr Leu Leu I Ie gga gaa cgc ttc aaa 21 G31y (31u Ar g Phe Lys at c ct g gaa gac t gg Ilie Leu (31u Asp Tr p tac tcc cac ccc aac Tyr Ser Hi s Pr o Asn cga ggt cgt t cc t cc Ar g G31y Ar g Ser Ser cca gt c t cc t cc gac Pr o Val Ser Ser Asp 1 00 at g acc agt t ca aga I'b t Thr Ser Ser Ar g 115 gga aaa O3 y Lys 130 gcc acc cga Ala Th r Ar g Page 332 seqI i st car r ect ed. t xt ggc gag ct a t ct aaa gt g gaa cga acc G31y G31u Leu Ser Lys Val G31u Ar g Thr 135 140 ccc gca Pro Al a cca t gg ct g aca gt c Pro Trp Leu Thr Val ggc gcc aaa aca cta gcc G31y Ala Lys Thr Leu Al a 150 155 gcc aaa tca aac gga ttc Al a Lys Ser Asn G3 y Phe 1 70 gtt cta gaa ggc gcc acc Val Leu 31u G31y Al a Thr 1 85 atc cgc acc cct tca ccc Ilie Ar g Thr Pr o Ser Pr o 200 gca gca ct c t tt gca cac Al a Al a Leu Phe Al a Hi s 215 aaa gac t ta agc at t gac Lys Asp Leu Ser Ilie Asp 230 235 gt g t cc t cc gt c cgc gga Val Ser Ser Val Ar g G31y 250 aaa tta cgg aaa cca gac Lys Leu Arg Lys Pro Asp 265 aaa gct ct g ggg t agaggct Lys Al a Leu G3 y 280 <210> 260 <211> 281 <21 2> PRT <21 3> Cor ynebact er i urn gi <400> 260 I'bt Val Phe Trp Asp Asp 1 5 G31u Th r Leu Leu I Ie Ar g G31y G31u Ar g Phe Lys Al a Ilie Leu G31u Asp Tr p G31u Tyr Ser Hi s Pr o As n Al a 70 Ar g G31y Ar g Ser Ser Thr Pr o Val Ser Ser Asp Lys 1 00 t ac Tyr g ac As p t cc Ser ggt G31y gca Al a 220 g at As p cca Pr o aat As n gca Al a g at As p ac c Thr ggc G31y 205 ac c Thr ct t Le u gt t ValI g aa (31u gca Al a gt g ValI gt a ValI 1 90 g ac As p g aa G31u tt c Ph e cgg Ar g aaa Lys5 270 aac As n at c I Ie 1 75 gt g ValI at t I Ie aaa Lys5 gga G y gt g ValI 255 g aa 0 u at g 1 60 Ph e agt Ser ct c Le u gga G31y gcc Al a 240 ac c Thr at c I Ie gca Al a ac c Thr tt c Ph e ccc Pr o t gg Tr p 225 g ac As p ag g Ar g aag Lys5 gcc Al a g at As p aaa Lys5 gga G31y 210 cga Ar g ag c Ser ct c Le u gcg Al a cgc Ar g g ac As p 1 g ac As p ac c Thr aaa Lys5 t gg Tr p gga G31y 260 at t I Ie t ac Tyr 1 cgc Ar g aaa Ly s C aa (31n g aa G31u ct a Le u 245 C ac Hi s ac c Thr gg cgct gggact t gc ut ani cumn Al a Al a Leu As p 03 y Hi s Se r Al a Al a 40 Lys Al a Thr 03 y 03 u Al a 03 y Leu Al a Leu Al a 03 n 1 Thr Ar gI 10 Al a Cys Leu Leu (31n db t Ser Cys Ser 03 y 90 Ar g 031u I Page 333 As p ValI Le u li1e Tr p Le u G31y lIe Ar g 031u Se r Le u I Ie Se r Met Gy Pr o 145 Al a Thr Phe Pr o Tr p 225 Asp Ar g Lys Ser 115 Al a Leu Leu Gy Gy 195 Thr Cys Val Asp Leu 275 Ar g Ar g Val Tyr 165 Ar g Lys G n G u Leu 245 Hi s Thr Tyr G u 135 Al a Lys Leu Ar g Al a 215 Asp Ser Leu Al a seql i st cor r ect ed. t xt Ser Ile Asp Thr y Leu 120 125 Leu Ser Lys Val G u Arg 140 Lys Thr Leu Ala Tyr Ala 155 Ser Asn G y Phe Asp Asp 170 G u G y Al a Thr Ser Thr 185 Thr Pro Ser Pro Gy y 200 205 Leu Phe Ala His Ala Thr 220 Leu Ser lie Asp Asp Leu 235 Ser Val Arg Gy Pro Val 250 Arg Lys Pro Asp Asn G u 265 Leu G y 280 Pr o Thr Al a Val Val 190 Asp G u Phe Ar g Lys 270 Gy Pr o Asn SIe 175 Val SIe Lys Gy Val 255 G u <210> 261 <211> 1224 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1201) <223> RXN01690 <400> 261 cctagccatt cctcaaaacc gtgagacgaa att actagtgtat ctgtcaggta gcaggtgtac ctt ttc Phe gaa Gu gt g Val gt g Val t ac gt a Val ctt Leu at t SIle t ac Tyr cag cgt Ar g gca Al a tgg Tr p ccg Pr o at t gaa Gu aag Lys gag Gu cct Pr o gag ccg Pr o ggt Gy gaa Gu gat Asp at t ggct at t cat cccat aa aat ggggct g aaaatcc atg acg tca tta gag IVet Thr Ser Leu G u 1 acg tca ccc gat cgt ctg aag Thr Ser Pro Asp Arg Leu Lys 15 aag ttc ttc acc gac cac atg Lys Phe Phe Thr Asp Hi s IVet ggc tgg cac aac gcc caa tta G y Trp His Asn Ala G n Leu cct gcc acc acc gta ttc cac Pro Ala Thr Thr Val Phe His aag gcc tac cgc cat tcg gac Page 334 Tyr gaa G u cgt Ar g at t SIle gag G u acc Thr 150 gt c Val gtt Val act Thr cag G n gcc Al a 230 ttc Phe t cc Ser gca Al a gag G u tgc Cys 310 cac Hi s Sy n Ala Ile Phe u G seql i st cor r ect ed. t xt y Ile Lys Ala Tyr Arg His Ser at c SIle gca Al a gca Al a 120 ggc Gy at t SIle gca Al a gt c Val gac Asp 200 cag G n gag G u t ac Tyr t ca Ser gac Asp 280 gaa G u act Thr acc Thr act Thr cga Ar g gaa G u gaa G u ttg Leu cca Pr o 170 ct g Leu aaa Lys gcg Al a aag Lys aac Asn 250 ctt Leu gga G y gac Asp gct Al a gaa 33u 330 tt c Phe at g Met ct g Leu gct Al a ggt G y 155 gt c Val agc Ser ttt Phe gaa G u tac Tyr 235 ggc G y cca Pr o tac Tyr gca Al a gtt Val 315 gt g Val cgt Ar g gca Al a ct g Leu t cc Ser 140 gt c Val ggc G y gaa G u gct Al a aag Lys 220 at c SIle gac Asp ggc G y gaa G u aag Lys 300 at c SIle aac Asn cct Pr o at g Met gt a Val 125 ct c Leu agc Ser gct Al a gat Asp ggc G y 205 ggc G y gaa G u caa G n at c SIle gt a Val 285 tct Ser acc Thr aac Asn gat Asp cca Pr o 110 gac Asp t ac Tyr cca Pr o t ac Tyr t ac Tyr 190 aac Asn tgt Cys gaa G u gt c Val acc Thr 270 gaa G u ggc Gy cct Pr o aat Asn gaa G u 95 cag G n gcg Al a ct g Leu gct Al a ttc Phe 175 gt c Val t ac Tyr gac Asp at g Met aag Lys 255 cgc Ar g gag G u gcc Al a gtt Val gaa 33u 335 aac Asn ttg Leu gat Asp cgc Ar g gat Asp 160 acc Thr cgc Ar g gcg Al a cag G n ggt G y 240 ct a Leu aag Lys cga Ar g at g Met ggc G y 320 gt c Val gcc Al a cca Pr o cag G n cca Pr o 145 gcc Al a ggt G y gct Al a gct Al a gt c Val 225 ggc G y gt c Val t ca Ser aag Lys acc Thr 305 acc Thr gga G y gag G u acc Thr gat Asp 130 ttc Phe t ac Tyr gga G y gca Al a tct Ser 210 gt a Val at g Met acc Thr ctt Leu at c SIle 290 gag G u gt g Val gaa G u cgt Ar g gag G u 115 tgg Tr p at g Met aag Lys at c SIle ccc Pr o 195 ttg Leu tgg Tr p aac Asn cct Pr o ct a Leu 275 acc Thr gca Al a aaa Lys at c l Ie at g Met 100 gac Asp gtt Val at c SI e ttc Phe aag Lys 180 ggc G y ctt Leu ttg Leu ctt Leu gaa G u 260 caa G n acc Thr ttt Phe t ca Ser acg Thr 340 cag G n ttt Phe cct Pr o t cc Ser ct g Leu 165 cct Pr o gga G y gcc Al a gat Asp ggg G y 245 ctt Leu gt a Val acc Thr gct Al a gct Al a 325 at g Met 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 aag ctt cgt gaa acc ctc acc gga att cag caa gga aac gtt gaa gac Page 335 seqi i st car r ect ed. t xt Lys Leu Arg Ou Thr Leu Thr Oy lieOn On Oy Asn Val Ou Asp 345 350 355 caa aac gga tgg ct t t ac cca ct g gt t ggc t aaat caacc ggtt t taaga O31n As n O31y Tr p Leu Tyr Pro Leu Val O31y 360 365 ccc <210> 262 <211> 367 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1221 1224 <400> 262 l'bt Thr Ser Leu 03 u Phe Thr Val Thr Pr o Ph e Hi s Thr Tyr A a Pr o O31n Pr o 145 A a O31y A a A a ValI 225 O31y ValI Ar g As p A a ValI Hi s Ar g O31u 115 Tr p vb t Ly s I Ie Pr o 1 95 Le u Tr p As n Pr o Ly s vb t Le u Hi s As p O31n Ph e Pr a Ser Le u 1 65 Pr a O31y A a As p O31y 245 Le u O31u ValI ValI Tyr 70 O31u Ar g I Ie O31u Thr 150 ValI ValI Thr O31n A a 230 Ph e Ser I Ie Thr Pr a O31y Thr Ser Ly s Tyr 135 O31u I Ie Ser O31y Ser 215 le le O31y Le u I Ie 40 Tyr O31n I Ie A a A a 120 Oy le A a ValI As p 200 O31n O31u Tyr Ser A a As p A a A a Ly s A a 1 Le u O31y O31y Ser Tr p 1 A a A a Hi s Ar g Le u 265 Ar g 10 A a Tr p Pr a I Ie Thr 90 Ar g O31u O31u Le u Pr a 1 70 Le u Ly s A a Ly s As n 250 Le u Thr Pr a As n I Ie Ph e 75 Ph e vb t Le u A a O31y 1 55 ValI Ser Ph e O31u Tyr 235 O31y Pr a O31u Ly s O31u Pr a O31u Ar g A a Le u Ser 140 ValI O31y O31u A a Ly s 220 li1e As p O31y As n Ph e Ser vb t O31y Pr a vb t ValI 125 Le u Ser A a As p O31y 205 O31y O31u O31n I Ie Thr Ly s O31y Pr a Ly s O31u O31n A a Le u A a Ph e 1 ValI Tyr As p vb t Ly s 255 Ar g Ser Ph e Tr p A a A a As n Le u As p Ar g As p 1 Thr Ar g A a O31n O31y 240 Le u Ly s Ser Leu Leu O31n Val Ala Ar g Asp Leu O31y Tyr O31u Val O31u O31u Ar g Page 336 Lys I I e 290 Thr 03 u 305 Thr Val G31y (1u 31y As n 275 Thr Thr Thr A a Phe A a Lys Ser A a 325 1I1 e Thr l'b t 340 Val 31u As p 355 a3 u Tr p 295 Cy s (1y 310 Hi s G3 y Lys Leu 31n As n seqi i st car r ect ed. t xt 280 285 G3 u G3 u Asp A a Lys Ser 300 Thr Ala Ala Val I Ie Thr 315 Thr Phe a3 u Val As n As n 330 Ar g a31u Thr Leu Thr a31y 345 a31y Tr p Leu Ty r Pr o Leu 360 365 a3 y A a [vb t Pr o Val G31y 320 As n a3 u Val 335 li1e a31n a31n 350 Val a31y <210O> 263 <21 1> 1076 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1053) <223> FRXA01690 <400> 263 ccc Pr o Ph e c ac Hi s ac c Th r t ac Ty r gcc A a cca Pr o c ag a31n cca Pr o gcc A a 145 cgt Ar g g ac As p gcc A a gt a ValI cat Hi s cgt Ar g gag a31u t gg Tr p 115 at g vb t aag Ly s aag Ly s at g vb t tt a Le u c ac Hi s g ac As p c ag a31n tt t Ph e cct Pr o t cc Ser ct g Le u gaa a31u gt g ValI gt g ValI t ac Tyr gaa a31u 70 cgt Ar g at t I Ie gag a31u acc Thr gt c ValI 150 at t I Ie ac c Thr cca Pr a gga a31y ac c Thr t ca Ser aaa Ly s t ac Tyr g aa a31u 135 at c I Ie ct t Le u at t I Ie t ac Tyr 40 c ag a31n at c I Ie gca A a gca A a ggc a31y 120 at t I Ie gca A a gcc A a g ac As p gcg A a gca A a aag Ly s gct A a ct t Le u 1 05 gga a31y a31y t cc Ser gca A a 10 t gg Tr p ccg Pr a at t I Ie act Thr cga Ar g 90 g aa a31u g aa a31u tt g Le u cca Pr a ccg Pr a aac As n at t I Ie tt t Ph e tt c Ph e at g vb t ct g Le u gct A a ggt a31y gt c ValI 1 tt c Ph e t cg Ser at g vb t gga a31y cct Pr a at g vb t gt a ValI ct c Le u 125 ag c Ser gct A a ggt a31y g aa a31u g at As p at t I Ie g at As p cca Pr a g ac As p 110 t ac Tyr cca Pr a t ac Tyr aag Ly s ggc a31y cct Pr a aag Ly s g aa a31u c ag a31n gcg A a ct g Le u gct A a tt c Ph e Page 337 seql i st cor r ect ed. t xt ggt Gy gct Al a gct Al a gt c Val ggc G y 225 gt c Val t ca Ser aag Lys acc Thr acc Thr 305 gga Gy gga G y gga at c G y lle gca ccc Ala Pro tct ttg Ser Leu 195 gta tgg Val Trp 210 atg aac IVet Asn acc cct Thr Pro ctt cta Leu Leu atc acc Ile Thr 275 gag gca G u Al a 290 gtg aaa Val Lys gaa at c G u Ile aac gtt Asn Val cct Pr o 165 gga G y gcc Al a gat Asp ggg G y ctt Leu 245 gt a Val acc Thr gct Al a gct Al a at g Met 325 gac Asp gtt tcc gtc Val Ser Val act Thr cag G n gcc Al a tt c Phe 230 t cc Ser gca Al a gag G u tgc Cys cac Hi s 310 aag Lys caa ggt G y t cc Ser at c l Ie 215 at c SIle ggc G y cgc Ar g tgg Tr p ggt Gy 295 ggc Gy ctt Leu aac tgg ctg agc Trp Leu Ser 170 gcc aaa ttt Ala Lys Phe 185 gct gcg gaa Al a Al a G u cac aag tac Hi s Lys Tyr cgc aac ggc Arg Asn y 235 ct a ctt cca Leu Leu Pro 250 ttg gga tac Leu G y Tyr 265 gaa gac gca G u Asp Al a gca gct gtt Al a Al a Val ttc gaa gtg Phe G u Val 315 gaa acc ct c G u Thr Leu 330 gaa gat tac G u Asp Tyr gct ggc aac Al a y Asn 190 aag ggc tgt Lys G y Cys 205 atc gaa gaa lie G u G u 220 gac caa gt c Asp G n Val ggc at c acc G y I e Thr gaa gta gaa Su Val u 270 aag t ct ggc Lys Ser G y 285 at c acc cct I e Thr Pr o 300 aac aac aat Asn Asn Asn acc gga att Thr G y Ile gt c Val 175 t ac Tyr gac Asp at g Met aag Lys cgc Ar g 255 gag G u gcc Al a gtt Val gaa G u cag G n 335 cgc Ar g gcg Al a cag G n ggt G y ct a Leu 240 aag Lys cga Ar g at g Met ggc G y gt c Val 320 caa G n 528 576 624 672 720 768 816 864 912 960 1008 1053 1076 aaa t aa Sn Asn y Trp 345 ctt tac cca ctg Leu Tyr Pro Leu gtt ggc Val G y 350 taaatcaacc ggttttaaga ccc <210> 264 <211> 351 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 264 Pro Asp Arg Leu Lys u Ile Leu Ala Ala Pro Lys Phe y Lys Phe 1 5 10 Phe Thr Asp Hi s IVet Val Thr II e Asp Trp Asn G u Ser G u G y Trp 25 His Asn Ala G n Leu Val Pro Tyr Ala Pro Ile Pro IVet Asp Pro Ala 40 Thr Thr Val Phe His Tyr G y G n Ala lie Phe G u G y lie Lys Ala Page 338 seqI i st car r ect ed. t xt Ar g Pro Asp (31u Tyr Al a Pr o O31n Pr o Al a 145 G31y Al a Al a ValI G31y 225 ValI Ser Ly s Thr Thr 305 G31y (31y Hi s Ser As p Ar g [Vbt (31n 03 u As p Phe 1 00 Tr p Val Pr o 115 I'b t I Ie Ser Lys Phe Leu Ilie Lys Pr o 1 65 Pro G31y G31y 1 80 Leu Leu Al a 1 95 Trp Leu Asp Asn Leu 03 y Pro G31u Leu 245 Leu 03 n Val 260 Thr Thr Thr 275 Al a Phe Al a Lys Ser Al a Il e Thr I'b t 325 Val 31u Asp 340 Thr I Ie Lys Thr Ser Ly s Ty r G31u 135 li1e Ser G31y Ser I Ie 215 li1e G31y Ar g Tr p G31y 295 G31y Le u As n Al a Al a G31y 120 li1e Al a ValI As p O31n 200 O31u Ty r Ser As p O31u 280 Th r Th r Ar g G31y Al a Le u 1 G31y G31y Ser Tr p Al a 1 Al a Hi s Ar g Le u Le u 265 O31u Al a Ph e O31u Tr p 345 Ph e Le u Al a G31y ValI 1 Ser Ph e O31u Ty r G31y 235 Pr o Ty r Al a ValI ValI 315 Le u Ty r Al a Le u Ser ValI 140 G31y O31u Al a Ly s I Ie 220 As p G31y O31u Ly s I Ie 300 As n Th r Pr o ValI Le u 125 Ser Al a As p G31y G31y 205 O31u O31n I Ie ValI Ser 285 Th r As n G31y Le u O31n Al a Le u Al a Ph e ValI 1 Ty r As p vb t Ly s Ar g 255 O31u Al a ValI O31u O31n 335 G31y <210O> 265 <21 1> 1782 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1759) <223> RXN01026 <400> 265 Page 339 seql i st cor r ect ed. t xt gaggtgacat ttatgcctct taatagctat actgtcccat gaaacgatat tttaatctca tatagtgaga tttacagatt tttaaaggac ggtgagttcc atg acc agc ccc gtg lMt Thr Ser Pro Val gag G u cgc Ar g t ac Tyr gac Asp ct g Leu t ca Ser ct g Leu gat Asp acc Thr cac Hi s 150 gag G u at g Met gac Asp ggc Gy gat Asp 230 agc Ser cat Hi s gac Asp ct g Leu acc Thr ct g Leu gac Asp cga Ar g 120 cca Pr o gct Al a gt c Val at t SIle at t SIle 200 gtt Val cgc Ar g t ca Ser gt g Val cag G n at g let gac Asp at c SIle tgt Cys ggc Gy at g Met ggc Gy gct Al a 170 gtt Val gcg Al a gaa G u acc Thr act Thr t cc Ser ct g Leu acc Thr cac Hi s 75 aac Asn gaa G u at c SIle acc Thr t cc Ser 155 act Thr act Thr at t l Ie tac Tyr at g Met 235 gag G u aag Lys ct g Leu ggc Gy aac Asn gac Asp gaa G u gt g Val at t SIle 140 at g let caa G n ggt Sy at c SIle cgc Ar g 220 tgc Cys aag Lys gga Sy cat Hi s 45 cgt Ar g gt g Val aag Lys ttc Phe cac Hi s 125 gt g Val gca Al a acc Thr gaa G u gcc Al a 205 ggc Sy aac Asn ct g Leu gaa G u gaa G u aaa Lys cca Pr o at t SIle ggc G y 110 acc Thr tgc Cys ttc Phe ct g Leu ct g Leu 190 aag Lys gaa G u at g Met acc Thr 15 aac Asn gt g Val ct g Leu acc Thr t cc Ser 95 gt g Val gt c Val ggt G y ggc Gy cca Pr o 175 cag G n at c SIle gca Al a t cc Ser ct g Leu ggc Gy acc Thr cgc Ar g gaa G u 80 cgc Ar g cgc Ar g ggc Gy gac Asp at c SIle 160 ct g Leu cca Pr o ggc Gy at c l Ie at c SIle 240 1 gca Al a gag G u t ca Ser cac Hi s ggc Gy ct g Leu ct g Leu cca Pr o t cc Ser 145 ggt Gy aag Lys ggt Gy acc Thr cgt Ar g 225 gaa G u gag G u ccc Pr o cca Pr o cca Pr o at c SIle cag G n cac Hi s cag G n 130 cac Hi s acc Thr cct Pr o gtt Val ggc Sy 210 aag Lys gct Al a aag Lys gac Asp cag G n gaa G u aag Lys gt a Val cca Pr o 115 ct c Leu acc Thr t ca Ser ttc Phe t cc Ser 195 ggc Sy at g Met ggc Gy gt g Val ct c Leu gca Al a ct g Leu act Thr tct Ser 100 at g let ggc G y t cc Ser gag G u aag Lys 180 t cc Ser gga Sy t cc Ser gca Al a tgg Tr p ct c Leu ttt Phe cac Hi s ggc Sy act Thr ggt G y gca Al a acc Thr gtt Val 165 acc Thr aag Lys cag G n at g Met cgt Ar g 245 gcc ggc atg atc gcc cca gac caa acc acc Ala y IVet lie Ala Pro Asp n Thr Thr ttc gac tac gtt gaa ggc Phe Asp Tyr Val G u y Page 340 seqi i st car r ect ed. t xt cgc Ar g t gg Tr p gaa O31u cca Pr o 310 ttc Phe at g lvb t gt c Val gcc Al a at g lvb t 390 gga G31y ggc G31y gag O31u cca Pr o gaa O31u 470 aaa Lys agc Ser agc Ser at g lvb t acc Thr 280 gat Asp cag O31n aac Asn ct g Leu ct g Leu 360 gac Asp gt g Val gac Asp t cc Ser t cc Ser 440 ggc G31y gcg Al a t gg Tr p cca Pr o t ca Ser 250 cca Pr o cca Pr a t cg Ser ct g Leu aac Asn 330 cca Pr a t cc Ser ct C Leu t cc Ser at c l Ie 410 t gc Cys ttc Phe acc Thr cct Pr a aat Asn 490 t gg Tr p gga G31y aag Lys acc Thr gca Al a cca Pr a 315 gac Asp gga G31y t gc Cys aag Lys t cc Ser 395 tt C Phe ct g Leu acc Thr cac Hi s gt c Val 475 tt a Leu aca Thr ccg Pr a ggc G31y gac Asp ct g Leu 300 ct g Leu aag Lys acc Thr acc Thr ggc G31y 380 acc Thr acc Thr ggc G31y t cc Ser ct g Leu 460 ct C Leu cca Pr a ccg Pr a gct Al a gcc Al a gaa O31u 285 acc Thr ggc G31y gca Al a cca Pr a aac Asn 365 cac Hi s t gg Tr p gac Asp at g lvb t aac Asn 445 gt a Val acc Thr cct Pr a acc Thr t cg Ser gac Asp 270 ggc G31y cca Pr a gaa O31u gca Al a ct g Leu 350 gcc Al a aaa Lys at c l Ie gct Al a aac Asn 430 cga Ar g t cc Ser t gc Cys aca Thr aga Ar g 510 aag Lys 255 t gg Tr p gca Al a ttc Phe t cc Ser gcc Al a 335 cgc Ar g cgc Ar g at c l Ie aag Lys ggc G31y 415 cca Pr a aac Asn cca Pr a aga Ar g ccg Pr a 495 t ca Ser gac Asp acc Thr at c l Ie gt a Val 320 gaa O31u gac Asp at c l Ie gcc Al a caa O31n 400 gct Al a gac Asp ttc Phe gca Al a t at Tyr 480 gcg Al a t cc Ser gaa O31u ttt Phe acc Thr 305 cca Pr a aag Lys at c l Ie gaa O31u gac Asp 385 gag O31u gaa O31u caa O31n gaa (31u gt c Val 465 ct a Leu ttg Leu cag O31n gca Al a gac Asp 290 t gg Tr p agc Ser gca Al a aag Lys gac Asp 370 ggc O31y gca Al a t gg Tr p ct g Leu gga O31y 450 gca Al a agg Ar g gcg Al a ccg Pr a gt t Val 275 aag Lys ggc O31y cca Pr a ct g Leu at c l Ie 355 ct g Leu at g lvb t gaa O31u cgt Ar g aag Lys 435 cgc Ar g gcc Al a aag Lys ttc Phe t ct Ser 515 260 gct Al a gt c Val acc Thr gaa O31u cag O31n 340 gac Asp cag O31n cgc Ar g gcg Al a acc Thr 420 cca Pr a caa O31n gcc Al a gct Al a cac Hi s 500 acc Thr 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 acg gac tgt Thr Asp Cys Page 341 ttt cca act Phe Pr o Thr seqI i st car r ect ed. t xt 520 525 530 gcc aaa acg acc cca act ttg tcc tca aca ccg aca cct aca aga acg Ala Lys Thr Thr Pro Thr Leu Ser Ser Thr Pro Thr Pro Thr Arg Thr 535 540 545 gct ccg t tc tcg tagcaggccc t gact t tggc acc Ala Pr o Phe Ser 550 <210> 266 <211> 553 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1 747 1 782 <400> 266 I'bt Thr Ser Pro Val 03 u As n Se r A a O31u Ser Hi s G31y Le u Le u Pr o Ser 145 G31y Ly s G31y Thr Ar g 225 O31u As p Ly s As p O31n O31u Ly s ValI Pr o 115 Le u Thr Ser Ph e Ser 1 95 G31y vb t G31y ValI Tr p Le u Ph e Hi s G31y Thr G31y A a Thr ValI 1 65 Thr Ly s O31n vb t Ar g 245 G31y Ar g Tyr As p Le u 70 Ser Le u As p Thr Hi s 150 O31u vb t As p G31y As p 230 A a Ar g As p I Ie G31y A a Le u Ar g ValI O31n 135 G31y Hi s A a Le u Tyr 215 A a G31y (31u Thr Ser 10 Val Val Leu 03 n Ar g [Vb t 31u As p O31u I Ie 90 As n Cys 1 05 O31n (1y 31y Ib t Phe 03 y [vb t A a 1 70 O3 u Val 1 85 Leu A a Leu 03 u I'b t Thr I e A a 250 Ala Pr o 265 Thr Ser Le u Thr Hi s 75 As n O31u I Ie Thr Ser 1 55 Thr Thr I Ie Tyr vb t 235 Pr o Ly s G31y Hi s Ar g ValI Ly s Ph e Hi s 125 ValI A a Thr O31u A a 205 G31y As n O31n Leu Thr Leu 31u As n (1y (31u Val Thr Lys Leu Arg Pr o Thr O31u Ilie Ser Ar g (31y Val Ar g 110 Thr Val O31y Cys (1y Asp Phe 03 y Il e 1 Leu Pro Leu 1 Leu O31n Pr o 1 Lys IlIe 03 y (3 u A a IlIe I'b t Ser Ilie 240 Thr Thr Phe 255 Asp Trp Asp 270 Lys 03 y A a Page 342 s G u Ala Val Ala Tyr Trp Lys Thr 275 280 eql i st cor r ect ed. t xt Leu Pro Thr Asp G u 285 G y Al a Thr Phe Thr 305 Pr o Lys SIle G u Asp 385 G u G u G n G u Val 465 Leu Leu G n Cys Thr 545 Lys Gy Pr o Leu SIle 355 Leu let G u Ar g Lys 435 Ar g Al a Lys Phe Ser 515 Pr o Thr Val Asn Asp 325 Tyr Thr SIle let Leu 405 Al a Gy Gy Thr Ar g 485 Cys Ser Gy Thr u Pr o 310 Phe Met Val Al a Met 390 Gy Gy G u Pr o G u 470 Lys Ser Ser Al a Al a 550 SIle 295 Gy Thr Asp Phe Al a 375 Val Leu Cys Ar g Gy 455 Ser G n Asp Al a Lys 535 Pr o Asp G n Asn Leu Leu 360 Asp Val Asp Ser Ser 440 Gy Al a Tr p Pr o Ser 520 Thr Phe Gy Gy Asp Val 345 Gy SIle Pr o Lys let 425 Al a Ar g Asp Lys Thr 505 Pr o Thr Ser Al a Pr o 315 Asp Gy Cys Lys Ser 395 Phe Leu Thr Hi s Val 475 Leu Thr Pr o Thr Leu 300 Leu Lys Thr Thr Gy 380 Thr Thr Gy Ser Leu 460 Leu Pr o Pr o Al a Leu 540 Thr Gy Al a Pr o Asn 365 Hi s Tr p Asp Met Asn 445 Val Thr Pr o Thr Ser 525 Ser Pr o G u Al a Leu 350 Al a Lys SIle Al a Asn 430 Ar g Ser Cys Thr Ar g 510 Lys Ser Phe Ser Al a 335 Ar g Ar g SIle Lys Gy 415 Pr o Asn Pr o Ar g Pr o 495 Ser Thr Thr SIe Val 320 G u Asp SIe Al a G n 400 Al a Asp Phe Al a Tyr 480 Al a Ser Asp Pr o <210> 267 <211> 1625 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(1602) <223> FRXA01026 <400> 267 gtg tgg cgc gac cat gtc gtg tcc aag gga gaa aac ggc gag ccc gac Val Trp Arg Asp His Val Val Ser Lys Gy G u Asn Gy G u Pro Asp Page 343 seql i st cor r ect ed. t xt 1 5 10 ctc ctc tac atc gac ctg cag ctg ctg cat gaa gtg acc tca cca cag 96 Leu Leu Tyr Ile Asp Leu G n Leu Leu His G u Val Thr Ser Pro G n 25 gca ttt gac ggc ctg cgc atg acc ggc cgt aaa ctg cgc cac cca gaa 144 Al a Phe Asp G y Leu Arg IVet Thr G y Arg Lys Leu Arg Hi s Pro G u 40 ctg cac ctg gcc acc gaa gac cac aac gtg cca acc gaa ggc atc aag 192 Leu His Leu Ala Thr G u Asp His Asn Val Pro Thr G u y lie Lys 55 act ggc tca ctg ctg gaa atc aac gac aag att tcc cgc ctg cag gta 240 Thr y Ser Leu Leu G u Ile Asn Asp Lys Ile Ser Arg Leu G n Val 70 75 tct act ctg cgc gac aac tgt gaa gaa ttc ggc gtg cgc ctg cac cca 288 Ser Thr Leu Arg Asp Asn Cys G u G u Phe G y Val Arg Leu His Pro 90 atg ggt gat gtc cga cag ggc atc gtg cac acc gtc ggc cca cag ctc 336 Met G y Asp Val Arg n G y IIe Val His Thr Val G y Pro G n Leu 100 105 110 ggc gca acc cag cca ggc atg acc att gtg tgc ggt gac tcc cac acc 384 G y Ala Thr G n Pro G y Iet Thr IIe Val Cys G y Asp Ser His Thr 115 120 125 tcc acc cac ggt gct ttt ggc tcc atg gca ttc ggc atc ggt acc tca 432 Ser Thr His G y Ala Phe G y Ser IVet Ala Phe G y IIe G y Thr Ser 130 135 140 gag gtt gag cac gtc atg gct act caa acc ctg cca ctg aag cct ttc 480 G u Val G u His Val IVet Ala Thr G n Thr Leu Pro Leu Lys Pro Phe 145 150 155 160 aag acc atg gcc att gaa gtt act ggt gaa ctg cag cca ggt gtt tcc 528 Lys Thr IVet Al a I e G u Val Thr G y G u Leu G n Pro G y Val Ser 165 170 175 tcc aag gac ctg att ctg gcg att atc gcc aag atc ggc acc ggc ggc 576 Ser Lys Asp Leu Ile Leu Ala lie lie Ala Lys Ile y Thr y y 180 185 190 gga cag ggc tac gtt ctg gaa tac cgc ggc gaa gca atc cgt aag atg 624 Sy n y Tyr Val Leu u Tyr Arg y u Ala lie Arg Lys IVet 195 200 205 t cc atg gat gca cgc atg acc atg tgc aac atg tcc atc gaa gct ggc 672 Ser IVet Asp Ala Arg IVet Thr IVet Cys Asn IVet Ser Ile G u Ala y 210 215 220 gca cgt gcc ggc atg atc gcc cca gac caa acc acc ttc gac tac gtt 720 Al a Arg Al a G y Iet I e Al a Pro Asp G n Thr Thr Phe Asp Tyr Val 225 230 235 240 gaa ggc cgc gaa atg gca cca aag ggc gcc gac tgg gac gaa gca gtt 768 G u G y Arg G u IVet Al a Pro Lys G y Al a Asp Trp Asp G u Al a Val 245 250 255 gct tac tgg aag acc ctg cca acc gac gaa ggc gca acc ttt gac aag 816 Ala Tyr Trp Lys Thr Leu Pro Thr Asp G u y Ala Thr Phe Asp Lys 260 265 270 gtc gta gaa atc gat ggc tcg gca ctg acc cca ttc atc acc tgg ggc 864 Val Val G u Ile Asp G y Ser Ala Leu Thr Pro Phe Ile Thr Trp y Page 344 seql i st cor r ect ed. t xt 0 285 275 acc aac cca Thr Asn Pro 290 gaa gac ttc G u Asp Phe 305 cag tac at g G n Tyr 'bet gac acc gt c Asp Thr Val cag atc gcc G n Ile Ala 355 cgc atg at g Arg vebt Vet 370 gcg ctc gga Al a Leu G y 385 acc gca ggc Thr Al a G y cca ggc gag Pro G y G u caa gga cca Sn G y Pr o 435 gcc acc gaa Al a Thr G u 450 gct aga aaa Al a Ar g Lys 465 cac tgc agc Hi s Cys Ser acc tca agc Thr Ser Ser act ggc gcc Thr G y Al a 515 aga acg gct Arg Thr Ala 530 <210> 268 cag G n aac Asn ct g Leu 325 ct g Leu gac Asp gt g Val gac Asp t cc Ser 405 t cc Ser ggc Sy gcg Al a tgg Tr p cca Pr o 485 t ca Ser acg Thr ggc G y gac Asp 310 gt a Val gga Sy at c SIle cct Pr o aaa Lys 390 at g bet gca Al a cgc Ar g gac Asp aaa Lys 470 acg Thr ccc Pr o acc Thr ct g Leu 295 aac Asn cca Pr o t cc Ser ct c Leu t cc Ser 375 at c SIle tgc Cys ttc Phe acc Thr cct Pr o 455 aat Asn tgg Tr p gga Sy cca Pr o cca Pr o gac Asp gga Sy tgc Cys aag Lys 360 t cc Ser ttc Phe ct g Leu acc Thr cac Hi s 440 gt c Val tta Leu aca Thr ccg Pr o act Thr 520 ct g Leu aag Lys acc Thr acc Thr 345 ggc Sy acc Thr acc Thr ggc G y t cc Ser 425 ct g Leu ct c Leu cca Pr o ccg Pr o gct Al a 505 ttg Leu ggc G y gca Al a cca Pr o 330 aac Asn cac Hi s tgg Tr p gac Asp at g Met 410 aac Asn gt a Val acc Thr cct Pr o acc Thr 490 tcg Ser t cc Ser gaa G u gca Al a 315 ct g Leu gcc Al a aaa Lys at c SIle gct Al a 395 aac Asn cga Ar g t cc Ser tgc Cys aca Thr 475 aga Ar g aag Lys t ca Ser t cc Ser 300 gcc Al a cgc Ar g cgc Ar g at c SIle aag Lys 380 ggc G y cca Pr o aac Asn cca Pr o aga Ar g 460 ccg Pr o t ca Ser acg Thr aca Thr gt a Val gaa G u gac Asp at c SIle gcc Al a 365 caa G n gct Al a gac Asp ttc Phe gca Al a 445 t at Tyr gcg Al a t cc Ser gac Asp ccg Pr o 525 cca Pr o aag Lys at c SIle gaa G u 350 gac Asp gag G u gaa G u caa G n gaa G u 430 gt c Val ct a Leu ttg Leu cag G n tgt Cys 510 aca Thr agc Ser gca Al a aag Lys 335 gac Asp ggc Sy gca Al a tgg Tr p ct g Leu 415 gga G y gca Al a agg Ar g gcg Al a ccg Pr o 495 ttt Phe cct Pr o cca Pr o ct g Leu 320 at c SIe ct g Leu at g Met gaa G u cgt Ar g 400 aag Lys cgc Ar g gcc Al a aag Lys ttc Phe 480 t ct Ser cca Pr o aca Thr 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1625 ccg ttc tcg Pro Phe Ser tagcaggccc tgactttggc acc Page 345 seqI i st car r ect ed. t xt <21 1> 534 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 268 Val Tr p Ar g Leu Leu Tyr Al a Phe Asp Leu Hi s Leu Thr G3 y Ser Ser Thr Leu [Vb t 31y Asp G3 y Al a Thr 115 Ser Thr Hi s 130 31u Val G31u 145 Lys Thr I'b t Ser Lys Asp (31y (1n (1y 1 95 Ser I'b t Asp 210 Al a Ar g Al a 225 G31u G31y Ar g Al a Tyr Tr p Val Val G31u 275 Thr Asn Pr o 290 G3 u Asp Phe 305 G3 n Tyr I'b t Asp Hi s Val Val As p Le u Thr Le u As p Ar g Pr a Al a ValI I Ie 1 65 li1e ValI Ar g vb t vb t 245 Thr As p a31n As n Le u 325 a31n vb t As p 55 li1e cys a31y vb t a31y 135 Al a ValI Al a a31u Thr 215 Al a Pr a Pr a Ser Le u 295 As n Pr a Ser Lys G31y 10 Leu Leu Hi s Thr a31y Ar g 40 Hi s Asn Val Asn Asp Lys a3 a 3 u Phe 90 Ilie Val Hi s 1 Thr Ilie Val 120 Ser I'b t Al a Thr a3 n Thr Thr a3 y a3 u 1 70 Ile IlIe Al a 1 Tyr Ar g a31y 200 I'b t Cys Asn Pr o Asp a31n Lys a3 y Al a 250 Thr Asp a3 u 265 Al a Leu Thr 280 Pr o Leu a31y Asp Lys Al a a31y Thr Pr o (31u a31u Lys5 Pr a I Ie 75 a31y Thr Cys Ph e Le u 1 55 Le u Lys5 a31u vb t Thr 235 As p a31y Pr a a31u Al a 315 As n ValI Le u Thr Ser ValI ValI a31y a31y 140 Pr a a31n I Ie Al a Ser 220 Thr Tr p Al a Ph e Ser 300 Al a G31y Thr Ar g a31u Ar g Ar g a31y As p 125 li1e Le u Pr a ay le 205 li1e Ph e As p Thr I Ie 285 ValI a31u Pr o Asp Pro a31n Pro a31u I e Lys a3 n Val Hi s Pr o a3 n Leu Hi s Thr Thr Ser Pr o Phe 1 Val Ser 1 a31ya(1y Lys I'b t Al a a3 y Ty r Val 240 Al a Val 255 As p Lys Trp a31y Ser Pr o Al a Leu 320 Lys I I e 335 Leu Arg Asp Ilie Page 346 seql i st cor r ect ed. t xt Asp Thr Val Phe Leu G y Ser Cys Thr Asn Ala Arg II e G u Asp Leu 350 340 G n Ar g Al a 385 Thr Pr o G n Al a Al a 465 Hi s Thr Thr Ar g Al a 355 Ibet Gy Gy G u Pr o 435 G u Lys Ser Ser Al a 515 Al a Asp Val Asp Ser 405 Ser Gy Al a Tr p Pr o 485 Ser Thr Phe Leu Ser 375 I le Cys Phe Thr Pr o 455 Asn Tr p Gy Pr o 345 Gy Thr Thr Gy Ser 425 Leu Leu Pr o Pr o Al a 505 Leu Lys SIle Al a 395 Asn Ar g Ser Cys Thr 475 Ar g Lys Ser Al a 365 G n Al a Asp Phe Al a 445 Tyr Al a Ser Asp Pr o 525 Gy Al a Tr p Leu 415 Gy Al a Ar g Al a Pr o 495 Phe Pr o <210> 269 <211> 1143 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1120) <223> RXN01127 <400> 269 gccctgcatg atggggtagt gggggttgtt gggcaggtac cact agt gaa gt ccat at ag t gagaaggga at cccacaac gagct gt gat caat cagct a atg aaa ctt gct gtt 115 att ggt gga lie G y y gtt ct a aac Val Leu Asn ggc gca cgc Sy Al a Ar g gat ggt atc ggc cca gag Asp Gy lie Gy Pro Gu gct gtc cgc gac gac atc Ala Val Arg Asp Asp Ile 30 cgt tac ctc aaa aat ggc Arg Tyr Leu Lys Asn Gy 45 Iet Lys Leu Ala Val 1 gtt act gca gaa gcc ctc aag 163 Val Thr Ala G u Ala Leu Lys 15 gag acc acc gat tat gac ctt 211 G u Thr Thr Asp Tyr Asp Leu gag ctg ctc acc gac gag gat 259 G u Leu Leu Thr Asp G u Asp Page 347 seql i st cor r ect ed. t xt ctg gca tcc ctg cgc gag cat gac gcg atc ctt ctt ggc gct atc ggt 307 Leu Ala Ser Leu Arg G u His Asp Ala lie Leu Leu G y Ala lie G y 60 gca cca ggt tcc gtc cct cca gga att ctc gag cgt ggt ttg ctg ctg 355 Ala Pro Gy Ser Val Pro Pro Gy lie Leu G u Arg Gy Leu Leu Leu 75 80 aag atg cga ttc gca ctg gat cac cac gtg aac ctg cgc cca tcc aag 403 Lys IVet Arg Phe Ala Leu Asp His His Val Asn Leu Arg Pro Ser Lys 95 100 ctg tac gac ggc gtg gag tcc cca ctg cgt aac cca ggc aag att gat 451 Leu Tyr Asp Gy Val u Ser Pro Leu Arg Asn Pro Gy Lys Ile Asp 105 110 115 ttc gtt gtg gtc cgc gaa ggt acc gaa ggc gcc tac act ggc aac ggt 499 Phe Val Val Val Arg G u Gy Thr G u Gy Ala Tyr Thr Gy Asn y 120 125 130 gga gca atc cgc gtg gga acc cct cac gag att gcc aat gaa acc tcc 547 Sy Ala Ile Arg Val y Thr Pro His u Ile Ala Asn u Thr Ser 135 140 145 gtg aac act cgc tac ggc gct gag cgc gtt att cgc tac gca ttc gag 595 Val Asn Thr Arg Tyr G y Ala G u Arg Val Ile Arg Tyr Ala Phe G u 150 155 160 165 ctg gca cag agc cgc cgc aag aag ctc acc ctc gtg cac aag acc aac 643 Leu Ala G n Ser Arg Arg Lys Lys Leu Thr Leu Val His Lys Thr Asn 170 175 180 gtc ctg gtt cac ggt ggt ggc ctg tgg cag cgc acc gta gat gag gtt 691 Val Leu Val His G y G y G y Leu Trp G n Arg Thr Val Asp G u Val 185 190 195 gca aag gaa tac cca gag gta gcc gtc gat tac aac cac atc gat gca 739 Ala Lys G u Tyr Pro G u Val Ala Val Asp Tyr Asn His lie Asp Ala 200 205 210 gca acc atc tat ctg gtc act gat cct tcc cgc ttc gat gtg att gtt 787 Ala Thr Ile Tyr Leu Val Thr Asp Pro Ser Arg Phe Asp Val lie Val 215 220 225 acc gat aac ctc ttc ggc gac atc ctc acc gat gag gca ggc gca gtc 835 Thr Asp Asn Leu Phe G y Asp Ile Leu Thr Asp G u Ala G y Al a Val 230 235 240 245 tct ggc gga att ggc ctc gca gca tcc ggc aac atc gat gcc acg ggc 883 Ser G y G y lie G y Leu Al a Al a Ser G y Asn Ile Asp Ala Thr G y 250 255 260 acc aac cct tcc atg ttc gag cca gtc cac ggc tct gca cca gat atc 931 Thr Asn Pro Ser IVet Phe G u Pro Val His Gy Ser Ala Pro Asp lie 265 270 275 gca ggc cag gga atc gca gac cca acg gca gca atc cta tcc gct gcg 979 Ala G y On G y lie Ala Asp Pro Thr Ala Ala lle Leu Ser Ala Ala 280 285 290 atg ctg ctg cgt cac tta ggt gat gaa gac aac gca gta cgt att gaa 1027 IVet Leu Leu Arg His Leu y Asp G u Asp Asn Ala Val Arg Ile G u 295 300 305 aca gcc atc gca gct gat gtg gct ggc cga gat aac tct cag ccg att 1075 Thr Ala Ile Ala Ala Asp Val Ala y Arg Asp Asn Ser n Pro lie 310 315 320 325 Page 348 seqI i st car r ect ed. t xt tct acc act gag gt g gga gac cgc at c gt c aag gcg ct g caa agc Ser Thr Thr O31u Val G31y Asp Ar g I Ie Val Lys Al a Leu O31n Ser 330 335 340 t aaat t tcaa cgccgacccc ct t <210> 270 <211> 340 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1120 1143 <400> 270 I'bt Lys Leu Al a Al a 0u Al a Leu Thr Asp Tyr As p Leu Thr As p 03 u Leu 03 y Al a I I e Ar g G31y Leu Leu Leu Ar g Pr o Ser 1 00 Pro G31y Lys I Ie 115 Ty r Thr 03 y As n 130 Al a As n 03 u Thr 145 Ar g Ty r Al a Phe Val Hi s Lys Thr 1 80 Thr Val As p (31u 1 95 As n Hi s I I e As p 210 Phe As p Val I Ie 225 03 u Al a 03 y Al a I I e As p Al a Thr 260 Ser Al a Pr o As p 275 ValI Ly s Le u As p G31y Le u Ly s As p G31y Ser 031u 1 65 As n ValI Al a ValI ValI 245 Gy le I I e 03 y 03 y Asp ValI G31y Le u Al a 70 Ly s Le u Ph e G31y ValI 150 Le u ValI Al a Al a Thr 230 Ser Thr Al a Le u Al a Al a 55 Pr o vb t Tyr ValI Al a 135 As n Al a Le u Ly s Thr 215 As p G31y As n (31y Al a Ar g Le u Ser Ph e G31y 1 ValI Ar g Ar g Ser Hi s 1 Tyr Tyr Le u I Ie Ser 265 (31y I Ie Ar g Le u 031u Pr o 75 Le u 031u 031u G31y G31y 1 55 Ar g G31y 031u ValI G31y 235 Le u Ph e G31y As p Ly s Hi s Pr o As p Ser G31y Thr 140 Al a Ly s G31y ValI Thr 220 As p Al a 031u Pr o As p As n As p G31y Hi s Pr a Thr 125 Pr a 031u Ly s Le u Al a 205 As p I Ie Al a Pr a ValI 031u 0u le Le u ValI Ar g G31y O31u ValI Thr 1 O31n As p Ser Thr G31y 255 Hi s Thr Thr Le u Le u O31u As n As n Al a I Ie I Ie 1 Le u Ar g Tyr Ar g As p 240 As n (31y Ilie Ala Asp Pro Thr Ala Ala 285 Page 349 seql i st cor r ect ed. t xt I e Leu Ser Ala Al a Iet Leu Leu Arg Hi s Leu G y Asp G u Asp Asn 290 295 300 Ala Val Arg I le u Thr Ala I e Al a Ala Asp Val Ala G y Arg Asp 305 310 315 320 Asn Ser G n Pro lie Ser Thr Thr G u Val y Asp Arg lie Val Lys 325 330 335 Ala Leu G n Ser 340 <210> 271 <211> 403 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(403) <223> FRXA01132 <400> 271 gccctgcatg atggggtagt gggggttgtt gggcaggtac cact agt gaa gt ccat at ag t gagaaggga at cccacaac at t SIle gtt Val ggc Gy ct g Leu gca Al a aag Lys gga Gy aac Asn cgc Ar g t cc Ser ggt Gy cga Ar g ggt Gy gt c Val t ac Tyr cgc Ar g gt c Val gca Al a ggc Gy gac Asp aaa Lys cat Hi s cca Pr o gat Asp cca Pr o gac Asp aat Asn 45 gac Asp gga Gy cac Hi s gag Gu at c I e ggc Gy gcg Al a at t SIle cac Hi s act Thr acc Thr ct g Leu ctt Leu gag G u 80 aac Asn gagctgtgat caatcagct atg aaa ctt gct gtt IVet Lys Leu Ala Val 1 gca gaa gcc ctc aag Ala G u Ala Leu Lys acc gat tat gac ctt Thr Asp Tyr Asp Leu ctc acc gac gag gat Leu Thr Asp G u Asp ctt ggc gct atc ggt Leu G y Al a lie G y cgt ggt ttg ctg ctg Arg y Leu Leu Leu ctg cgc cca tcc aag Leu Arg Pro Ser Lys 100 a 115 163 211 259 307 355 403 <210> 272 <211> 101 <212> PRT <213> Corynebact <400> 272 IVet Lys Leu Al a 1 Ala G u Ala Leu Thr Asp Tyr Asp er i um gl ut ai cum Val Ile Gy Gy Asp 5 Lys Val Leu Asn Al a 25 Leu G y Ala Arg Arg Gy lie y Pro u Val 10 Val Arg Asp Asp Ile u Tyr Leu Lys Asn Gy G u Page 350 seqI i st car r ect ed. t xt 40 Leu Thr Asp Ou Asp Leu Ala Ser Leu Arg Ou His Asp Ala Ilie Leu 55 Leu (1y Al a Ile3y Ala Pro (1y Ser Val Pro Pro(3y Ilie Leu(3u 70 75 Ar g O31y Leu Leu Leu Lys I'b t Ar g Phe Ala Leu Asp His His Val Asn 90 Leu Ar g Pr o Ser Lys 1 00 <210O> 273 <21 1> 1494 <21 2> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (1471) <223> RXN00536 <400> 273 cggcgggt cc cagaggtct t aacacgaccg gcat cccgt c t cgt ggaccc acccaaaact tt t taagaag gt tgaacaca gcggagt t tg gt gt tgccgg gca Al a aac As n gt t ValI ccg Pr o t gt cys t ct Ser tt c Ph e g at As p ac c Th r at c G31y cgc Ar g cgc Ar g gt t ValI gag O31u g aa O31u gt t ValI 120 c ag O31n gca Al a cca Pr o at g vb t t gg Tr p ct g Le u aag Lys5 gag O31u gag O31u ct g Le u cct Pr o gca Al a cct Pr o cca Pr o cgt Ar g 75 cgc Ar g gt c ValI at c I Ie gt t ValI gcc Al a t gg Tr p tt c Ph e g at As p g ac As p cgc Ar g ggt G31y at c I Ie c ag O31n 140 aag Lys5 aat As n gag O31u 45 aaa Lys5 G31y at g vb t tt c Ph e g aa O31u 125 gct Al a at c I Ie aag Lys5 gt t ValI aaa Lys5 aac As n tt t Ph e cct Pr a 110 aag Ly s cgt Ar g g aa O31u 15 c ag O31n gag O31u at c I Ie c ag O31n gag O31u 95 t ca Ser ggc O31y gag O31u ac c Th r cgt Ar g gt a ValI ac c Th r gct Al a 80 ct g Le u gct Al a at g vb t c ac Hi s at g vb t 1 cca Pr a O31y g aa O31u gt t ValI ct g Le u ct g Le u t cc Ser at c I Ie ct g Le u 145 t ct cct aac gat Ser Pr o As n As p gt t ggg cct cgc Val O31y Pro Ar g t cc t ca at g cca Ser Ser I'b t Pr o gat at t t ct ct g As p I Ie Ser Leu gca cct cag t gg Al a Pro O31n Tr p at t gat ccg at g Ilie Asp Pr o I'b t gt t cag at g ggc Val O31n [Vb t 31y 1 00 cag act gat ttt O3 n Thr Asp Phe 115 cct gac gat gt c Pr o Asp Asp Val 130 at t cgc cgt act Ilie Ar g Ar g Thr Page 351 seqi i st car r ect ed. t xt t tt gaa gct t gc gaa Phe G3 u A a Cys G3 u 1 50 ggc gca aaa aac gtt G3 y A a Lys Asn Val 155 at c gt g I I e Val cac t tc t ac aac Hi s Phe Tyr Asn t cc Ser c ag O31n gct A a t cc Ser gt t ValI 230 ct g Le u at t I Ie t cc Ser ct g Le u aac As n 310 at g vb t c ag O31n cgc Ar g c ac Hi s c ag O31n 390 ac c Thr t cc Ser aag Ly s g at As p 200 ac c Thr gag O31u t cc Ser t gg Tr p c ac Hi s 280 t ac Tyr gag (31u ac c Thr cgc Ar g cca Pr o 360 g ac As p ggt G31y at g vb t ct g Le u 1 70 ct g Le u cca Pr o act Thr at g vb t gt t ValI 250 c ac Hi s c ac Hi s gct A a ac c Thr ggc G31y 330 ac c Thr ggc G31y gt g ValI ag c Ser ggt G31y 410 cgc Ar g ac c Thr ac c Thr gt t ValI 220 cca Pr o at g vb t aat As n g ac As p gct A a 300 aac As n g ac As p g aa (31u g ac As p aag Ly s 380 act Thr tt a Le u aac As n g at As p aac As n 205 gag (31u act Thr at c I Ie ct a Le u cgt Ar g 285 g ac As p gt c ValI cct Pr o t ac Tyr ct g Le u 365 ggt G31y g aa (31u cct Pr o gt g ValI gcc A a 1 90 t gg Tr p t ac Tyr cct Pr a ac c Thr aac As n 270 99c G31y cgc Ar g t gc Cys c ag O31n t gc Cys 350 gt c ValI ct g Le u gt t ValI gcc A a 1 tt c Ph e g aa O31u t gg Tr p aag Ly s aac As n 240 aac As n cgt Ar g ggc O31y g aa O31u gt c ValI 320 g ac As p c ag O31n ac c Thr gcc A a t gg Tr p 400 cga Ar g cgc Ar g ct a Le u c ag O31n g aa O31u 225 cca Pr a gt t ValI g at As p gt t ValI O31y 305 ac c Thr tt c Ph e ct g Le u gct A a at g vb t 385 gag O31u t cc Ser at g at c t ac Tyr 210 gt t ValI at g vb t t ac Tyr t cc Ser ggc O31y 290 t gc Cys ct g Le u ac c Thr cgc Ar g tt c Ph e 370 gct A a c ag O31n aaa Ly s g ac As p aag Ly s 1 t cc Ser gt g ValI at c I Ie gca A a at t I Ie 275 gca A a ct g Le u gca A a g at As p gt t ValI 355 t cc Ser gcc A a ct g Le u gga (31y aag Ly s 1 ac c Thr cct Pr a g ac As p at c I Ie g ac As p 260 at c I Ie gct A a tt c Ph e ct g Le u at a I Ie 340 cct Pr a ggt O31y aag Ly s cgc Ar g t gt Cys 420 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 Page 352 seqI i st car r ect ed. t xt tcg cga cta cga ggc tgt tat ccg cgt gaa ctc cca gtc cgg caa ggg Ser Ar g Leu Ar g O31y Cy s Tyr Pr o Ar g O31u Leu Pro Val Arg O31n O31y 425 430 435 cgg cgt t gct t acat cat gaa gac cga tca cgg tct gca gat ccc tcg Ar g Ar g Cys Leu Hi s Hi s 03 u Asp Ar g Ser Ar g Ser Al a Asp Pr o Ser 440 445 450 ct c cat gca ggt t gagt t ct cc accgt t gt cc aga Leu Hi s Al a 03 y 455 <210> 274 <211> 457 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1411 1459 1494 <400> 274 vb t Pr a G y 0Ou ValI Le u Le u Ser I Ie Le u 145 ValI Ar g Le u 0On 0Ou 225 Pr a Ser Pr o As n Val G31y Pro Ser Ser [Vb t As p II e Ser Al a Pr o O31n I Ie As p Pr o Val (31nNb t 1 00 O3 n Thr As p 115 Pro Asp Asp 130 Ilie Ar g Ar g Hi s Phe Tyr Nib t Asp Lys 1 80 Il e Lys Thr 1 95 Tyr Ser Pr o 210 Val Val Asp 'b t Ilie Ilie As p Ar g Pr o Le u Tr p vb t G31y Ph e ValI Thr As n 1 65 ValI I Ie (31u Al a As n 245 Al a Phe Hie Ser (31u As n As p Al a Pr o Ly s Ph e I Ie 135 (31u Thr ValI O31n Ph e 215 ValI Pr o O31n 25 Tyr Thr As p Ar g I Ie 1 05 Ar g ValI Cys I Ie Ly s 1 85 Tyr O31y ValI Thr Al a 10 Pr o vb t Tr p Le u Ly s 90 O31u O31u Le u O31u Le u 1 70 Le u Pr o Thr vb t ValI 250 Pro Ala Lys Ilie O3 u Th r Al a Pr o Pr o Ar g Ar g ValI I Ie ValI O31y 1 O31n Al a As p O31u As p 235 O31u As n G u Ly s 0Oy vb t Ph e 0Ou 125 Al a Ly s As n As p As n 205 0Ou Thr I Ie Ly s ValI Ly s As n Ph e Pr o 110 Ly s Ar g As n ValI Al a 1 Tr p Tyr Pr o Thr O31n G u I Ie O31n O31u Ser O31y O31u ValI ValI 1 Al a Ar g Al a O31u Pr o 255 ValI Ty r Al a As p Se r I Ie O31u Tr p I'b t Hi s Ar g As n L eu As n Ar g Ar g Page 353 seql i st cor r ect ed. t xt 265 Asp Val Gy 305 Thr Phe Leu Al a bet 385 G u Ser Pr o Ser Ser Ile 275 G y Al a 290 Cys Leu Leu Al a Thr Asp Arg Val 355 Phe Ser 370 Al a Al a G n Leu Lys G y Val Arg 435 Al a Asp 450 I e Leu Ser Ala G u Leu Phe Gy Asn 310 Leu Asn IVet 325 Ile Arg n 340 Pro G u Ar g G y Ser Hi s Lys Val G n 390 Arg Asp Thr 405 Cys Arg Ser 420 Sn G y Ar g Pro Ser Leu Leu Hi s 280 Sy Tyr 295 G y G u Leu Thr li e Ar g His Pr o 360 Sn Asp 375 Pro G y Arg IVet Arg Leu Arg Cys 440 Pro His Asn Asp Mt Ar g G n Ser 345 Tyr Al a Al a Sy Ar g 425 Leu Al a Thr Sy 330 Thr Sy Val Ser Sy 410 G y Hi s Gy Sy 315 Val Val Sy Asn Ser 395 Ser Cys Hi s Al a 300 Asn Asp G u Asp Lys 380 Thr Leu Tyr G u Ar g 285 Asp Val Pr o Tyr Leu 365 Sy G u Pr o Pr o Asp 445 Sy Ar g Cys G n Cys 350 Val Leu Val Al a Ar g 430 Ar g Thr SIe Leu Leu 335 Asn Phe Asp Ser Tyr 415 G u Ser Hi s Al a G y 455 <210> 275 <211> 1333 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1333) <223> FRXA00536 <400> 275 cggcgggtcc cagaggtctt aacacgaccg gcatcccgtc tcgtggaccc acccaaaact ttttaagaag gttgaacaca gca ttc atc tcc gca cct gcc aag atc Ala Phe Ile Ser Ala Pro Ala Lys Ile aac gaa ggc cag cca gca tgg aat aag Asn Gu Gy n Pro Ala Trp Asn Lys 30 gtt aac cgc tac atg cct ttc gag gtt Val Asn Arg Tyr IVet Pro Phe G u Val 45 gaa acc G u Thr 15 cag cgt G n Arg gag gta G u Val Page 354 gcggagtttg gtgttgccgg atg tct cct aac gat IVet Ser Pro Asn Asp 1 cca gtt ggg cct cgc Pro Val y Pro Arg ggc tcc tca atg cca Sy Ser Ser Vet Pro gaa gat att tct ctg Su Asp lie Ser Leu seql i st cor r ect ed. t xt ccg gac Pro Asp tgt gct Cys Al a tct cct Ser Pro ttc aaa Phe Lys gat ttc Asp Phe acc att Thr Ile 135 ttt gaa Phe G u 150 t cc acc Ser Thr cag gtg G n Val gct cag Al a G n tcc ttc Ser Phe 215 gtt gtt Val Val 230 ct g cct Leu Pro att gaa Ile G u t cc ct g Ser Leu ctg ggc Leu G y 295 aac ggc Asn y 310 cgc act tgg cca gat Arg Thr Trp Pro Asp aaa aaa atc acc Lys Lys Ile Thr gtt gca cct cag tgg Val Ala Pro n Trp gtt Val gag G u gaa G u gtt Val 120 cag G n gct Al a t cc Ser aag Lys gat Asp 200 acc Thr gag G u t cc Ser tgg Tr p cac Hi s 280 t ac Tyr gag G u ct g Leu aag Lys gag G u gag G u ct g Leu gaa Gu ct g Leu 170 ct g Leu cca Pr o act Thr at g Met gtt Val 250 cac Hi s cac Hi s gct Al a acc Thr gac Asp cgc Ar g ggt Gy at c SIle cag G n 140 gca Al a cgc Ar g acc Thr acc Thr gtt Val 220 cca Pr o at g Met aat Asn gac Asp gct Al a 300 aac Asn ggc Gy at g Met ttc Phe gaa G u 125 gct Al a aaa Lys aac Asn gat Asp aac Asn 205 gag G u act Thr at c SI e ct a Leu cgt Ar g 285 gac Asp gt c Val aac Asn ttt Phe cct Pr o 110 aag Lys cgt Ar g aac Asn gt g Val gcc Al a 190 tgg Tr p t ac Tyr cct Pr o acc Thr aac Asn 270 ggc Gy cgc Ar g tgc Cys cag G n gag G u 95 t ca Ser ggc Gy gag G u gtt Val gt g Val 175 gct Al a cgc Ar g gcc Al a gag G u cct Pr o 255 cgt Ar g acc Thr at c SIle ct g Leu gct Al a 80 ct g Leu gct Al a at g Met cac Hi s at c SIle 160 ttc Phe gaa G u tgg Tr p aag Lys aac Asn 240 aac Asn cgt Ar g ggc Gy gaa G u gt c Val 320 ct g Leu ct g Leu t cc Ser at c SIle ct g Leu 145 gt g Val cgc Ar g ct a Leu cag G n gaa G u 225 cca Pr o gtt Val gat Asp gtt Val ggc Gy 305 acc Thr at t SIle gtt Val cag G n cct Pr o 130 at t SIle cac Hi s at g Met at c SIle t ac Tyr 210 gtt Val at g Met t ac Tyr t cc Ser ggc Gy 290 tgc Cys ct g Leu gat Asp cag G n act Thr 115 gac Asp cgc Ar g ttc Phe gac Asp aag Lys 195 t cc Ser gt g Val at c SIle gca Al a at t SIle 275 gca Al a ct g Leu gca Al a ccg Pr o at g Met 100 gat Asp gat Asp cgt Ar g t ac Tyr aag Lys 180 acc Thr cct Pr o gac Asp at c SIle gac Asp 260 at c SIle gct Al a ttc Phe ct g Leu at g Mbt ggc G y ttt Phe gt c Val act Thr aac Asn 165 gt g Val at c SIe gag G u gca Al a aac Asn 245 t cc Ser ct g Leu gag G u ggc G y aac Asn 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 355 at g Met cag G n cgc Ar g cac Hi s cag G n 390 acc Thr acc Thr cgc Ar g cca Pr o 360 gac Asp ggt Gy tgg Tr p ggc Gy 330 acc Thr ggc Gy gt g Val agc Ser gtt Val 410 gac Asp gaa G u gac Asp aag Lys 380 act Thr seql i st cor r ect ed. t xt cct cag ctg gac ttc acc Pro G n Leu Asp Phe Thr 335 tac tgc aac cag ctg cgc Tyr Cys Asn G n Leu Arg 350 ctg gtc ttc acc gct ttc Leu Val Phe Thr Ala Phe 365 370 ggt ctg gac gcc atg gct G y Leu Asp Al a IVet Al a 385 gaa gtt tct tgg gag cag Su Val Ser Trp u n 400 gat Asp gtt Val 355 t cc Ser gcc Al a ct g Leu at a SIe 340 cct Pr o ggt Gy aag Lys cgc Ar g 1123 1171 1219 1267 1315 1333 <210> 276 <211> 411 <212> PRT <213> Corynebact <400> 276 IVet Ser Pro Asn 1 Pro Val y Pro G y Ser Ser IVet G u Asp Ile Ser Val Ala Pro G n Leu I e Asp Pro Leu Val G n IVet 100 Ser G n Thr Asp 115 lie Pro Asp Asp 130 Leu lie Arg Arg 145 Val Hi s Phe Tyr Arg IVet Asp Lys 180 er i um gl ut ai cum Asp Ala Phe Ile Ser Ala 5 10 Arg Asn Gu Gy Gn Pro 25 Pro Val Asn Arg Tyr IVet 40 Leu Pro Asp Arg Thr Trp 55 Trp Cys Ala Val Asp Leu 70 IVet Ser Pro G u Arg Lys 90 G y Phe Lys G u lie G u 105 Phe Asp Phe Val Arg G u 120 Val Thr Ile G n Val Leu 135 Thr Phe G u Ala Cys G u 150 Asn Ser Thr Ser Ile Leu 165 170 Val G n Val Lys Lys Leu 185 Pro Ala Lys Ile G u Thr Al a Pr o Pr o Ar g Ar g Val SIle Val Sy 155 G n Al a Tr p Phe Asp Asp Ar g Sy SIle G n 140 Al a Ar g Thr Asn G u Lys Sy et Phe G u 125 Al a Lys Asn Asp Lys Val Lys Asn Phe Pr o 110 Lys Ar g Asn Val Al a 190 G n Su I e G n G u Ser Sy G u Val Val 175 Al a Leu Ile Lys Thr lie Al a n Asp Tyr Pro Asp Thr Asn Trp Arg Trp Page 356 seqI i st car r ect ed. t xt 205 G3 u Tyr A a Lys a3 n Ty r 210 a3 u Val 225 Pr o Ib t Val Ty r Asp Ser Val G31y 290 31y Cys 305 Thr Leu Phe Thr Leu Arg A a Phe 370 [vb t A a 385 (31u (1n Se r Pro (31u Se r ValI I Ie A a I Ie 275 A a Le u A a As p ValI 355 Ser A a Le u A a As n 245 Ser Le u (31u G31y As n 325 Ar g G31u Ser ValI As p 405 Phe Thr G3 y 215 Val G31u Val Pr o Ser Thr a31u Tr p I'b t 265 Leu Hi s Pr o 280 a3 y Ty r I'b t 295 a31y a31u Ar g Leu Thr a3 n I Ie Ar g Ser 345 Hi s Pr o Ty r 360 a3 n As p A a 375 Pro a31y Ala a31u Tr p a31u Thr a3 u Val1 220 N/t As p Pr o 235 Val a31u It 250 Hi s Ar g As n Hi s As n As p A a a3 y A a 300 Thr a31y As n 315 a31yVal As p 330 Thr Val a31u a31ya1y As p Val Asn Lys 380 Ser Ser Thr 395 Val Pr o 410 Thr I Ie Le u Ar g 285 As p ValI Pr o Tyr Le u 365 a31y a31u G31u Pr o 255 Ar g Thr I Ie Le u Le u 335 As n Ph e As p Ser <210O> 277 <21 1> 714 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (691) <223> RXN02965 <400> 277 caggaggccg cacccacctg gtatccccag cagtcgcagc cgccaccgaa tccgcggacc ct gt cct cac ct gcagat at ct aaggaagg ct agaaaaca at g gaa aaa t t t acc I'b t a3 u Lys Phe Thr 1 acc tac acc ggc gtt ggc gtt cca ctg cag cga tcc aac gtg gac acc Thr Tyr Thr a31y Val a31y ValI Pro Leu a31n Ar g Ser Asn Val Asp Thr 15 gac cag atc atc cca gcc gtc tac ctc aag cgc gtc acc cgg acc ggc Asp a31n Ilie Ilie Pr o Ala Val Tyr Leu Lys Ar g Val Thr Ar g Thr a31y 30 t t cgaa gac gga ct gt tt t c caac tgg cgc caa aac gac ccc aac tt t Page 357 Phe G3 u Asp G3 y Leu Phe Ser Asn 45 eqI i st car r ect ed. t xt Trp Arg On Asn Asp Pro Asn Phe gt c ValI cct Pr o at g vb t tt c Ph e c ag O31n ct c Le u gt a ValI 1 50 g aa O31u g aa O31u aac As n tt t Ph e t ac Tyr G31y g ac As p 120 ct g Le u at c I Ie ct c Le u t ac Tyr g ac As p ac c Thr tt c Ph e t cc Ser g aa O31u gt g ValI tt c Ph e g ac As p 1 70 gct A a acc Thr ggc G31y 75 cgc Ar g gga G31y ct t Le u aac As n gaa O31u 155 gct A a aag Ly s t ac Tyr t cc Ser gct A a aaa Ly s ct g Le u ct g Le u 140 gt t ValI ggc G31y cgc Ar g aag Ly s t cc Ser gt c ValI gcg A a t gg Tr p 125 g aa O31u g ac As p ct g Le u cct Pr o aac As n cgc Ar g tt c Ph e ggc G31y 110 aag Ly s aag Ly s ccc Pr o ac c Thr gcg A a 1 90 t cc Ser c ac Hi s 80 t ca Ser ct c Le u at g vb t at c I Ie at c I Ie 1 60 cgc Ar g aag Ly s gt t ValI gcc A a cga Ar g ac c Thr g aa O31u gt c ValI 145 cgc Ar g aag Ly s cca Pr o ct c Le u gt c ValI tt c Ph e ggc G31y c aa O31n 130 ac c Thr t gg Tr p ct c Le u cgc Ar g gt a ValI t gg Tr p gcc A a at c I Ie 115 ac c Thr gca A a cgt Ar g g at As p act Thr 1 gca A a gca A a g ac As p 1 00 at g vb t cca Pr o G31y tt g Le u g aa O31u 1 aac As n ggc G31y ct c Le u at c I Ie g aa O31u G31y g ac As p at g vb t 1 at t I Ie gct A a t aagt t tcag t ct gat agcg aaa <210O> 278 <21 1> 197 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 278 I'b t O31u Lys Phe Thr Thr Tyr Thr G31y 1 5 Ser Asn Val Asp Thr AspO1n Ilie Ilie Val Thr Ar g Thr G31y Ph e O31u As p G31y 40 As n As p Pr o As n Phe Val Leu Asn Thr 55 Val Leu Val Ala G31y Pr o Asp Phe G31y 70 Ala Val Tr p Ala Leu I'b t Asp Tyr G31y Ar g Phe Ala Asp Ilie Phe Arg (31y As n 100 105 Val G31y Val 10 Pr o Ala Val Leu Phe Ser Asp Thr Tyr Thr 03 y Ser Phe Ar g Ala 90 Ser 03 y Lys Page 358 Pr a Tyr As n Ly s Ser ValI A a O31n Ly s Ar g G31y (31u Se r Thr (31u ValI 145 Ar g Ly s Pr o I Ie 115 Thr A a Ar g As p Thr 1 O31u G31y As p vb t 1 65 li1e A a seqI i st car r ect ed. t xt O31n Se r Asp Ilie u Leu Leu Tr p Lys Leu I'bt 120 125 Leu O31u Leu Thr Val As n Leu O31u Lys O31n I Ie 135 140 Val Val I Ie Ser Phe O31u Val Asp Pr o Ty r I Ie 150 155 160 O31u O31y Leu As p As p Ala O31y Leu Thr Leu Ar g 170 175 O31u As p Ty r O31u Ala Lys Ar g Pro Ala Phe Lys 185 190 <210O> 279 <21 1> 936 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> 101). (913) <223> RXN01929 <400> 279 aaaat t tgac t ccat aacga gaact taat c gagcaacacc ggaatt tat t t at tctgagc tggt catcacat ctat act c cct gaacagt gaat caaat c at t I Ie aac As n cgc Ar g gct A a g at As p gcg A a aat As n gct A a gca A a c ag O31n tt t Ph e aac As n at g vb t gt g ValI gcg A a gt g ValI 120 aaa Ly s gt t ValI gag (31u gt g ValI gt g ValI gt t ValI gag (31u at c I Ie cgc Ar g gt t ValI ggc G31y ggt G31y gcc A a ct g Le u gcg A a ggt (31y cgt Ar g ac c Thr g at As p g at As p gcg A a tt t Ph e cgg Ar g 110 gt g ValI tt c Ph e t at Tyr ct c Le u ac c Thr ac g Thr 80 ac c Thr at g vb t at c I Ie ccc at g t ca ggc Pr o I'bt Ser G31y gaa gct aaa gt a O3 u A a Lys Val gcg ct t t cg gcg A a Leu Ser A a gt t ggt gat t cc Val G31y Asp Ser t cg at c acc tt g Ser Ilie Thr Leu gct acg aag cgt Ala Thr Lys Ar g gag gt g agc cca O31u Val Ser Pr o 1 00 gaa acg ggt gcg O3 u Thr 03 y A a 115 cag acg at t cga (31n Thr Ilie Ar g 130 Page 359 seql i st cor r ect ed. t xt cgc Ar g ccg Pr o 150 gcg Al a ggt Sy gag Su aat Asn aac Asn 230 gat Asp gtt Val t cc Ser tct Ser ttt Phe acc Thr 200 aca Thr ggc G y ttg Leu gct Al a cat Hi s aag Lys 170 gtt Val gat Asp ggg G y aag Lys gac Asp 250 gga G y t cc Ser 155 ct c Leu gt g Val ctt Leu cag G n cca Pr o 235 gcc Al a att ccg gtt li e Pro Val 140 ttg ggc ggc Leu G y G y at c gcc gat I e Al a Asp ttg gag atg Leu G u IVet 190 t cc atc acc Ser Ile Thr 205 gtt ttg gtg Val Leu Val 220 cgc ttc gtc Arg Phe Val gcg cag gcc Ala G n Al a gtc ggc cE Val Gy Hi 1z cac gtg gt His Val VE 160 gcc cgc gc Al a Ar g Al 175 gtt cca gc Val Pro Al act at c gc Thr lie G tgg cag gc Trp n As 2, cgc gag tE Arg G u T) 240 tac atc gc Tyr Ile Al ac atc ggg s ie G y t cag ggt al G n Gy ;g t tg gag a Leu G u ;a gag gca a G u Al a 195 ja at c ggt y lie G y 210 at gcc ttc ip Al a Phe ac gcc acc yr Al a Thr cc gat at c a Asp Ile t ac Tyr cgt Ar g cag G n 180 gcg Al a gcc Al a ggc G y ttg Leu cac Hi s 260 acc Thr ggc G y 165 gcg Al a cgc Ar g ggc G y ct c Leu ggc Gy 245 gcg Al a ggt acc ttc cca ggc gaa gcg gag tcc Sy Thr Phe Pro Gy G u Ala u Ser 265 270 aag <210> 280 <211> 271 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 280 IVet Pro IVet Ser Gy Ile Asp Ala Lys 1 5 Arg u Ala Lys Val Asn Gy G n Lys 25 Asp Ala Leu Ser Ala Arg lie Phe Asp Leu Val y Asp Ser Al a Ala Asn Val 55 Leu Ser Ile Thr Leu Asp G u IVet lie 70 lie Ala Thr Lys Arg Ala Leu Val Val Tyr G u Val Ser Pro Asn G n Ala Val 100 105 Arg G u Thr y Ala Al a Ala Val Lys ttt Phe taatgcaggt agcaaccaca Lys II e Ar g 10 Val Ser Val G u Al a G y Val Leu G y Val Leu Al a Val Asp Leu 90 G u Ser Al a lie u G y Page 360 Ar g Thr Asp Asp Al a Phe Ar g 110 Val Hi s Ser Met Thr Val Sy Val G u Al a Hi s 145 Val Al a Al a Gy Asp 225 Tyr Al a 115 Thr Gy Gy G u Al a 195 Gy Phe Thr l Ie Ar g Thr Gy 165 Al a Ar g Gy Leu Gy 245 Al a Ar g Pr o 150 Al a Gy G u Asn Asn 230 Asp Gy SIle 135 G n Ser Al a Val Gy 215 Ar g Ser Thr seql i st cor r ect ed. t xt 120 125 Val Asp Ala Gy lie Pro 140 Ser G u His Ser Leu G y 155 Ser G y Lys Leu lie Al a 170 Phe Ala Val Val Leu G u 185 Thr G u Asp Leu Ser Ile 200 205 Thr Asp Gy G n Val Leu 220 Sy Lys Lys Pro Arg Phe 235 Leu His Asp Ala Ala G n 250 Phe Pro Gy Gu Ala u 265 Val Gy Asp bet 190 Thr Val Val Al a Ser 270 Val Hi s Al a 175 Val Thr Tr p Ar g Tyr 255 Phe <210> 281 <211> 930 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(907) <223> FRXA01929 <400> 281 tgactccata acgagaactt aatcgagcaa cacccctgaa tatttattct gagctggtca tcacatctat actcatgccc gca Al a cag G n ttt Phe aac Asn at g Met gt g aaa Lys gtt Val gag G u gt g Val gt g Val gtt cgc Ar g gtt Val ggc Gy ggt Gy gcc Al a ct g cgt Ar g acc Thr gat Asp gat Asp gcg Al a ttt ttc Phe t at Tyr ct c Leu acc Thr acg Thr acc cgc gaa Arg G u 15 gat gcg Asp Al a ctt gtt Leu Val ttg tcg Leu Ser at c gct I e Al a 80 tat gag Page 361 cagt gaat ca aat cggaat t atg tca ggc att gat IVet Ser Gy Ile Asp 1 gct aaa gta aac ggc Ala Lys Val Asn y ctt tcg gcg cgc att Leu Ser Ala Arg lle ggt gat t cc gct gcc G y Asp Ser Al a Al a atc acc ttg gat gag Ile Thr Leu Asp G u acg aag cgt gcg ctt Thr Lys Arg Ala Leu gtg agc cca aat cag seql i st cor r ect ed. t xt Val Val Val Asp Leu Pro Phe y Thr Tyr G u Val Ser 95 Pro Asn n 100 gcg Al a gt g Val gtt Val t cc Ser 150 tct Ser ttt Phe acc Thr aca Thr ggc G y 230 ttg Leu ttc Phe gag G u at c SIle 120 gct Al a cat Hi s aag Lys gtt Val gat Asp 200 ggg G y aag Lys gac Asp ggc G y gcg Al a ggt Gy at t l Ie ttg Leu at c SIle 170 ttg Leu t cc Ser gtt Val cgc Ar g gcg Al a 250 cgg Ar g gt g Val gtt Val 140 ggc Gy gat Asp at g Met acc Thr gt g Val 220 gt c Val gcc Al a at g Met 110 at c SIle ggc Gy gt g Val cgc Ar g cca Pr o 190 at c SIle cag G n gag G u at c l Ie cgt Ar g gcg Al a cac Hi s gtt Val gcg Al a 175 gca Al a gga G y gat Asp t ac Tyr gcc Al a 255 gaa G u cag G n at c SIle cag G n 160 ttg Leu gag G u at c SIle gcc Al a gcc Al a 240 gat Asp acg ggt gcg Thr G y Al a 115 acg att cga Thr I e Ar g 130 ggg tac acc G y Tyr Thr 145 ggt cgt ggc G y Arg G y gag cag gcg G u G n Al a gca gcg cgc Al a Al a Ar g 195 ggt gcc ggc G y Al a G y 210 ttc ggc ct c Phe G y Leu 225 acc tt g ggc Thr Leu G y atc cac gcg Ile His Ala gct Al a cgc Ar g ccg Pr o gcg Al a ggt Gy 180 gag G u aat Asn aac Asn gat Asp ggt G y 260 gcg gag tcc ttt Al a G u Ser Phe taatgcaggt agcaaccaca aag <210> 282 <211> 269 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 282 IVet Ser Gy Ile Asp Ala Lys Lys Ile 1 5 Ala Lys Val Asn Gy G n Lys Val Ser 25 Leu Ser Ala Arg lie Phe Asp G u Ala 40 G y Asp Ser Al a Al a Asn Val Val Leu 55 lie Thr Leu Asp G u Iet Ile Val Leu 70 Arg Thr Arg His Phe Arg u 10 Val Leu Thr Ser Tyr Asp Ala G y Val Asp IVet Leu Leu Val Sy Arg Asp Thr Thr Leu Ser Ala Lys Ala Val Thr Ile Ala 75 Page 362 seqI i st car r ect ed. t xt Thr Lys Ar g Al a Leu Val Val Val Asp Leu Pro Phe G31y Thr 90 Ty r 03 u ValI Thr Thr G31y 145 G31y O31u Al a G y Ph e 225 Thr I Ie Pr o Al a 115 Ar g Thr G31y Al a Ar g 1 95 G y Le u G31y Al a O31n Al a I Ie O31n Ser 1 65 Al a ValI G y Ar g Ser 245 Al a ValI ValI Ser 150 Ser Ph e Thr Thr O31y 230 Le u ValI Ly s As p 135 O31u O31y Al a O31u As p 215 Ly s Hi s Ser 1 O31u O31y Ser Le u ValI 1 Le u G n Pr o Al a Al a O31y I Ie Le u I Ie 1 70 Le u Ser ValI Ar g Al a 250 I Ie O31y Pr o O31y 1 55 Al a Ou le Le u Ph e 235 O31n ValI (31u 125 ValI Hi s Al a ValI Thr 205 Tr p Ar g Tyr Ar g Al a Hi s ValI Al a 1 Al a O31y As p Tyr Al a 255 (31u O31n I Ie O31n 1 Le u Ou le Al a Al a 240 As p Th r Ph e Pro O31y O31u Al a O31u Se r 265 <210O> 283 <21 1> 1065 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1042) <223> RXN01420 <400> 283 cgaagt tcac gact acaggc at tacct ggg t gct gat tt t gcggaat gcg ct gct cat cc acacgt ggaa t cct gat t gg tt g Le u ac g Th r aac As n ac a gt a ValI ccg Pr o t gg Tr p at t t cg Ser tt t Ph e tt t Ph e tt t t cg Ser ccg Pr a at c I Ie gcg ct g Le u tt a Le u ac c Thr 45 g ac ggc O31y gt g ValI tt t Ph e gga gcg gaa Al a 03 u 15 cgc aaa Ar g Lys gcc gt g Al a Val cca aag Page 36,: gcgcgagt aa agct gt ggct gt g acg ct g ggt gga Val Thr Leu O31y O31y 1 at g ct g t tc t ac ct g I'b t Leu Phe Tyr Leu gt g aag ggc gt g ggc Val Lys O31y Val O31y agc ct g gcg ct g at t Ser Leu Ala Leu Ilie ggg at t gag aac tt c seql i st cor r ect ed. t xt Thr Val IIe His Phe Tyr Ala Asp Gy Pro Lys Gy II 60 e G u Asn Phe ttt Phe cac Hi s tct Ser t ac Tyr aac Asn gct Al a 150 ct g Leu ggc G y at t SIle cag G n ttc Phe 230 ct g Leu aag Lys aaa Lys cgc Ar g acc Thr 310 cct Pr o gat Asp tgg Tr p ggt Gy 120 aac Asn tgg Tr p at g Met agt Ser ttt Phe 200 t at Tyr gca Al a acc Thr agt Ser ct c Leu 280 gag Gu ct g Leu gt g Val tct Ser ttt Phe acc Thr gt g Val ttt Phe 170 gaa Gu ttc Phe at t SIle gt g Val gtt Val 250 cgc Ar g ggc Gy ct c Leu tgg Tr p 75 tgg Tr p tac Ty r ggc Gy at c SIle cca Pr o 155 gtt Val at c SIle tac Ty r gct Al a tgt Cys 235 gat Asp tt g Leu aaa Lys aca Thr gac Asp ttt Phe t ac Tyr gcg Al a ccg Pr o 140 ct g Leu gt g Val gcc Al a at g Met ggc G y 220 ttc Phe gat Asp aag Lys agc Ser gaa G3u 300 acc Thr at g Met ttc Phe aag Lys 125 ct g Leu gca Al a gca Al a tcg Ser gt g Val 205 aaa Lys at c SIle cct Pr o cag G n ccc Pr o 285 aac Asn aat Asn cag G n ccg Pr o 110 ct g Leu at c SIle ttc Phe acg Thr cct Pr o 190 caa G n gaa G u gt g Val ttg Leu t cc Ser 270 gaa G u t cc Ser gt g Val gaa G u 95 ct g Leu gtt Val gca Al a aag Lys ctt Leu 175 cgc Ar g ttc Phe t ac Tyr tcg Ser at g Met 255 aat Asn aag Lys gaa G u t ca Ser gaa G u acc Thr gct Al a ct g Leu at g Met 160 gcg Al a gca Al a ccc Pr o ggc G y gt c Val 240 aag Lys at t SIle ccg Pr o gct Al a ccg Pr o at t SIle aga Ar g gaa G u gct Al a 145 tct Ser gt g Val gtt Val gt c Val ttc Phe 225 at t SIle gcc Al a ttg Leu tta Leu ccc Pr o 305 gt c Val ccc Pr o ct c Leu ggc Gy 130 gtt Val gt c Val aga Ar g ttg Leu at g Met 210 ctt Leu ct c Leu acg Thr gt c Val aaa Lys 290 gct Al a gcg Al a gt g Val at c SIle 115 at g Met tct Ser at c SIle gac Asp ttg Leu 195 gt g Val ggc G y gcg Al a gcg Al a cgt Ar g 275 gtt Val aag Lys gaa G u ct g Leu 100 gag G u ttt Phe ttt Phe at g Met at t SIe 180 ggt G y ttt Phe tgg Tr p tgg Tr p cgc Ar g 260 gac Asp gag G u gt a Val 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1065 gga atc aaa tct G y I e Lys Ser tagggaagga aaacatatgg ct a Page 364 seqi i st car r ect ed. t xt <210O> 284 <21 1> 314 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 284 Val Thr Leu G3 y ValI Ser G31y Pr o I Ie Ar g (31u A a 145 Ser ValI ValI ValI Ph e 225 li1e A a Le u Le u Pr o 305 Ph e G31y A a (31u A a ValI I Ie 115 vb t Ser I Ie As p Le u 1 95 ValI G31y A a A a Ar g 275 ValI Ly s G3 y Leu Leu Thr 31y As n I I e Thr Phe Phe 70 Val Hi s G3 u Ser Phe Tyr Lys As n Val Ala 150 Ser Leu 1 65 G31u (1y As n II e Val G3 n A a Phe 230 Val Leu 245 Lys Lys Leu Lys Thr Ar g A a Thr 310 As n Ph e Tr p ValI ValI A a Tyr Le u Thr 135 Thr Pr o Lys5 Ser Ar g 215 Ty r Ph e a31y ValI A a 295 a31y Pr o Le u A a Hi s Ar g Pr a Le u 1 ValI I Ie Ph e A a a31y 1 A a Ph e ValI Ph e Ar g 265 Ph e As n Tr p I Ie a31y Ty r Tr p 75 Tr p Tyr a31y I Ie Pr a 1 55 ValI I Ie Ty r A a cys 235 As p Le u Lys5 Thr Le u Le u Thr As p Thr vb t Ph e Lys5 125 Le u A a A a Ser ValI 205 Lys5 I Ie Pr a a31n Pr a 285 As n A a Ar g A a Pr a ValI a31u Le u ValI A a Ly s Le u 1 Ar g Ph e Tyr Ser vb t 255 As n Ly s a31u a31u Ly s ValI Ly s Ser a31u Thr A a Le u vb t 1 A a A a Pr a a31y ValI 240 Ly s I Ie Pr a A a <210O> 285 Page 365 seqI i st car r ect ed. t xt <21 1> 1137 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1114) <223> RXS01145 <400> 285 t aat gt agt t gt ct gcccaa gcgagt t aaa ct cccacgat ctt t tcacca aaat tt t tac gaaaggcgag att t tct ccc ct t Le u gcc Al a cgc Ar g t cc Ser gag O31u t cc Ser ggC G31y at c I Ie cca Pr o t gc CYS 1 50 ac c Thr cca Pr o gct Al a g at As p gt t ValI t ct Ser gag O31u gca Al a gca Al a gca Al a cca Pr o 120 Ca Hi s at c I Ie t cc Ser ac C Thr ct c Le u 200 g ac As p t ac Tyr gt t ValI gca Al a t gg Tr p at c I Ie ct g Le u g ac As p gt t ValI gtc ValI gca Al a 1 70 g aa O31u ggt G31y gct Al a ggC G31y gag G u aag Ly s gct Al a 75 t t c Ph e t t c Ph e gac As p cgc Ar g gac As p 155 gca Al a gct Al a ggC G31y g ac As p tcc Ser gt t ValI g aa O31u g ac As p c Thr ggc G31y at c I Ie cgt Ar g 140 c ag O31n gca Al a gag G31u c Thr tcc Ser ggc G31y at t G31y at c I Ie g ac As p ggc G31y 110 gt t ValI tt c Ph e cca Pr o ggt G31y gtc ValI 1 90 g aa (31u at c I Ie gca Al a ct g Le u gag G31u ct c Le u 80 gag G31u aac As n at g vb t g at As p gga G31y 1 60 gca Al a g ac As p gtc ValI ttacagtggg gggcagacat at g gct at t gaa ct g 11! I'b t Al a I I e 03 u Leu 1 cag ggc cgt aag gt t 1& O31n G31y Ar g Lys Val cac tcc cag aac ctc 21 Hi s Ser 03 n Asn Leu cgc gag ggc t cc aag Ar g G u G y Ser Lys gt C aag acc acc gct Val Lys Thr Thr Al a ct g gct cca gac acc Leu Ala Pro Asp Thr cca aac ctg aac gca Pro Asn Leu Asn Ala 1 00 at t cac t tc gac ct g Il e Hi s Phe Asp Leu 115 gtt gcg cca aag ggc 49! Val Al a Pr o Lys G31y 130 ggc aag ggt gt t cct 54' G31y Lys G31y Val Pr o 145 acc gca cag gct ct g 59! Thr Al a 03 n Al a Leu 1 cgc gca ggc gt t at c 64 Ar g Al a G31y Val Ilie 1 ctc ttc ggc gag cag 69 Leu Phe 03 y 03 u 03 n 1 aag gt t ggc t tc gag 73! Lys Val G31y Phe 031u 210 Page 366 seql i st cor r ect ed. t xt gtt ctc acc gaa gct ggc Val Leu Thr G u Ala G y 215 ctt cac gag ctc Leu His G u Leu 230 agc aac atg aac Ser Asn Ibet Asn ctc tcc ggc cca Leu Ser Gy Pro 265 gac atc ctg acc Asp Ile Leu Thr 280 gca aac gtt gag Ala Asn Val u 295 tac aac aac cac Tyr Asn Asn Hi s 310 atg agc tgg gtc bet Ser Trp Val ccctttgacg gct <210> 286 <211> 338 <212> PRT <213> Corynebact <400> 286 bet Ala I I e u 1 G n y Arg Lys His Ser G n Asn Arg G u G y Ser Val Lys Thr Thr Leu Ala Pro Asp Pro Asn Leu Asn 100 Ile His Phe Asp 115 Val Ala Pro Lys 130 aag Lys t ac Tyr 250 cgc Ar g gat Asp aac Asn cca Pr o aag Lys 330 tac gag Tyr G u 220 atc gtt Ile Val gtt tct Val Ser at c gat I e Asp cag gac Sn Asp 285 aac acc Asn Thr 300 gag gag Gu Gu gac gct Asp Al a cca gag atg gca tac ttc gag gtt Pro G u Met Ala Tyr Phe G u Val 225 gac Asp gac Asp gca Al a 270 ggc Gy gag G u acc Thr cgc Ar g ct c Leu acc Thr 255 gac Asp acc Thr ctt Leu ggc Gy gca Al a 335 at g Mt 240 gct Al a acc Thr ttc Phe gag G u gct Al a 320 gaa G u gaa G u ttc Phe t cc Ser aag Lys 290 ctt Leu ct c Leu gct Al a Leu G n Val Al a Val Asn Hi s SIle 125 G n ggt ggc at c G y y lie 245 ggt ggc tac G y G y Tyr 260 cgc atg aag Arg IVet Lys 275 cgc ct c at c Arg Leu Ile cgt gct t cc Ar g Al a Ser cgc gac ct c Arg Asp Leu 325 t aagt t t cac 835 883 931 979 1027 1075 1124 1137 er i um gl ut ai cum Leu Leu Tyr Asp Al a 5 Val Ala I e Val y Leu Arg Asp Ser y 40 Lys Ser Ala G u Lys 55 Al a G u Ala Ala Al a 70 Thr Ser G n Ala G u Al a y Asp Al a Leu 105 Leu I e Lys Pro Ala 120 G y Pro G y His Leu 135 Asp Al a Asp 10 Tyr G y Ser Val G u Val Al a Lys G u Trp Al a Asp II e Phe Thr 90 Leu Phe G y Asp Asp Ile Val Arg Arg 140 Page 367 Leu Hi s Gy Phe Met I e Leu Gy Val seql i st car r ect ed. t xt G31y 145 Thr Ar g Le u Ly s Al a 225 Ph e (31u Ly s Thr G31y 305 Ly s Thr Lys G31y Val Pro Cys Leu 1 1 150 e Al a Val O31n G31y G31y 1 95 G31y Ph e G31y G31y Ar g 275 Ar g Ar g Ar g Le u 1 65 li1e O31n O31u ValI I Ie 245 Tyr Ly s I Ie Ser Le u 325 Thr Pr o Al a ValI Le u 230 Ser Le u As p Al a Tyr 310 vb t Le u Thr ValI Le u 215 Hi s As n Ser I Ie As n 295 As n Ser Tyr Ph e 1 Cys O31u Le u As n Pr o 265 Thr O31u Hi s ValI As p 1 55 Al a Al a G31y G31y Le u 235 Ser ValI I Ie G31y I Ie 315 ValI O31n As p Pro Th r G31y 1 I Ie Thr O31u 205 O31u ValI Ser As p As p 285 Thr O31u Al a G31y 1 Thr Le u O31u Le u Thr 255 As p Thr Le u G31y Al a 335 Al a As p ValI vb t vb t 240 Al a Thr Ph e O31u Al a 320 O31u <210O> 287 <21 1> 556 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (556) <223> FRXA01145 <400> 287 t aat gt agt t gt ct gcccaa gcgagt t aaa ct cccacgat ctt t tcacca aaat tt t tac gaaaggcgag att t tct ccc ct t t at gat Leu Tyr Asp gcc atc gtt Al a IlIe Val cgc gat t ct Ar g Asp Ser gct gac gct gac ct c t cc Al a As p Al a Asp Leu Ser ggc tac ggc tcc cag ggc O3 y Tyr 03 y Se r 03 n 03 y 30 ggc gt t gag gt t gt c at t (3y Val (3u ValI Va I lie 45 t tg at c Leu I I e 15 cac gca Hi s Al a ggt ct g 03 y Leu Page 36E t tacagt ggg gggcagacat at g gct at t gaa ct g I'b t Al a I I e 03 u Leu 1 cag ggc cgt aag gtt O31n G31y Ar g Lys Val cac t cc cag aac ct C Hi s Ser 03 n Asn Leu cgc gag ggc t cc aag Ar g 31u G31y Ser Lys seql i tcc gca gag aag gca aag gaa gca ggc Ser Al a G u Lys Al a Lys G u Al a G y 60 gag gct gca gct tgg gct gac gtc atc G u Ala Ala Ala Trp Al a Asp Val lie 75 tcc cag gca gaa atc ttc acc aac gac Ser G n Ala G u Ile Phe Thr Asn Asp ggc gac gca ctg ctg ttc ggc cac ggc G y Asp Ala Leu Leu Phe G y His G y 105 110 atc aag cca gct gac gac atc atc gtt Ile Lys Pro Ala Asp Asp Ile Ile Val 120 125 cca ggc cac ttg gtt cgc cgt cag ttc Pro y His Leu Val Arg Arg G n Phe 135 140 t gc ctc atc Cys Leu Il e 150 <210> 288 <211> 152 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 288 IVet Ala lie G u Leu Leu Tyr Asp Al a 1 5 Sn y Arg Lys Val Ala I e Val y His Ser G n Asn Leu Arg Asp Ser G y 40 Arg G u y Ser Lys Ser Ala G u Lys Val Lys Thr Thr Ala G u Ala Ala Ala 70 Leu Ala Pro Asp Thr Ser G n Ala G u Pro Asn Leu Asn Ala G y Asp Ala Leu 100 105 lie His Phe Asp Leu Ile Lys Pro Ala 115 120 Val Ala Pro Lys G y Pro G y His Leu 130 135 Sy Lys y Val Pro Cys Leu Ile 145 150 <210> 289 st cor r ect ed. t xt ttc gag gtc aag Phe G u Val Lys atg ctc ctg gct IVet Leu Leu Al a 80 atc gag cca aac lie u Pro Asn 95 ctg aac att cac Leu Asn Ile Hi s ggc atg gtt gcg Sy lVbt Val Ala 130 gtt gat ggc aag Val Asp y Lys 145 acc Thr cca Pr o ct g Leu ttc Phe 115 cca Pr o ggt G y acc Thr gac Asp aac Asn 100 gac Asp aag Lys gtt Val gct Al a acc Thr gca Al a ctg Leu ggc G y cct Pr o Al a Sy G u Lys Al a Phe Phe Asp Ar g Leu G n Val Al a Val Asn Hi s SIle 125 G n Leu Hi s Sy Phe Met SIe Leu Sy Val Page 369 seqi i st car r ect ed. t xt <21 1> 1350 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1327) <223> RXA02375 <400> 289 ct t t t caggt ct acgt gt at acgat ggt aa cgct at gaat aagcgttcga ttacgggata atctcccaac gccaacccaa gatacgcaga acacacctga ggg G31y tt c Ph e g aa (31u aaa Ly s g at As p g aa O31u gga G31y aag Ly s gga G31y ac c Thr 1 50 ac c Thr aat As n ct t Le u cct Pr o gca Al a gga G y ggt G3y aac As n ggc G3y gcc Al a gct Al a 120 t ct Ser gct Al a g ac As p ac c Thr gca Al a 200 ac c Thr g at As p cgt Ar g t cc Ser at c I Ie g ac As p ct t Le u gca Al a gcc Al a gca Al a ct g Le u 1 70 gca Al a gt g ValI ccc Pr o ccg Pr o gag G u t ca Ser aac As n 75 ct c Le u gga G3y gca Al a cgg Ar g gga G3y 155 cgc Ar g acc Thr gcg Al a t ac Tyr g ac As p cgc Ar g tt g Le u act Thr at c I Ie tt g Le u gt g ValI t at Tyr 140 aag Ly s at t I Ie ac c Thr c ac Hi s c ag O31n aaa Ly s t cc Ser aac As n gt c ValI gt g ValI ac c Thr 110 c aa O31n cgc Ar g g at As p ggc G31y gt g ValI 1 90 gt g ValI g aa O31u 15 g ac As p aac As n g at As p aat As n t cc Ser 95 cgg Ar g gt t ValI ccc Pr o cgt Ar g gcg Al a 1 75 aat As n t cg Ser gca Al a ag c Ser gct Al a gag O31u gcc Al a 80 t ct Ser ccc Pr o c ac Hi s at c I Ie gcg Al a 1 60 gt t ValI tt t Ph e gct Al a at g gcg [vb t Al a 1 agc at c Ser I Ie gcc gca Al a Al a aag cga Lys Ar g gac gga As p (1y ttg caa Leu 03 n ggc gca O3 y Al a acg gaa Thr 03 u ct c at g Leu l'b t 130 ggc cag O31y (1n 145 agg aat Ar g As n cct at c Pr o I Ie ggt gac 31y As p gat gct As p Al a 210 ccg gt g aca Pr o Val Thr ggt gcg agc 03 y Al a Ser t ac gga cat Tyr 03 y Hi s gt g gt g gt g Val Val Val cac acc gt c Hi s Thr Val gca cgc at g Al a Ar g l'b t gt g gcc gcg Val Al a Al a 1 00 t tg gca gt c Leu Al a Val 115 cac cag t gg His O31n Tr p gt g ct t ct t Val Leu Leu gcg cag cgt Ala O31n Ar g 1 gt c aat gaa Val Asn (1u 1 aac gac cga Asn Asp Arg 1 t tg gt g ct g Leu Val Leu Page 370 seql i st cor r ect ed. t xt ctc agt gac gtg gat gga ctt ttt gat aaa aac cct act Leu Ser Asp Val Asp G y Leu Phe Asp Lys Asn Pro Thr gat ccc acc Asp Pro Thr 215 gcg aag Al a Lys 230 att gcc I e Al a gtg tct Val Ser acc tct Thr Ser act gt a Thr Val 295 gct ttg Al a Leu 310 gcg gtg Al a Val att act Ile Thr ttg gga Leu G y tct gat Ser Asp 375 gat ggc Asp y 390 gcc agc Al a Ser ttt att Phe I I e ggc gac Sy Asp gct gca Al a Al a 265 gcg gca Al a Al a 280 ttc cac Phe Hi s tat gcc Tyr Al a gaa gca G u Al a gaa at c G u Ile 345 cct gcc Pro Al a 360 acc ttg Thr Leu atg cag lMVt n cgc gcg Arg Ala tcc gag Ser G u 235 ggc gga Gy Gy 250 cgt ttg Arg Leu aac at t Asn II e ccc aag Pro Lys gca gat Al a Asp 315 gtg acc Val Thr 330 att ggt Ile G y ggc caa 3 y 3 n caa t ca G n Ser cgc ccg Arg Pro 395 220 gtt Val aaa Lys gct Al a ggc Gy gac Asp 300 act Thr t cc Ser gat Asp at c SIle at g Met 380 gt a Val cgt Ar g gt g Val t cc Ser cca Pr o 285 aac Asn gca Al a ggt Gy ttc Phe at c SIle 365 gtt Val gt g Val gac ggc Asp y ggc acc G y Thr 255 cga agt Arg Ser 270 gca ct g Al a Leu cgc ct c Arg Leu gga aag G y Lys ggt aaa G y Lys 335 cag cag G n G n 350 ggg cga G y Arg ggt atg G y let cat gca Hi s Al a aat Asn 240 ggt G y ggc G y gaa G u tcc Ser at c SIle 320 t ct Ser ggt Sy ggc Sy caa G n gat Asp 400 225 gat Asp ggc G y gt g Val gac Asp gcg Al a 305 cga Ar g ttg Leu gag G u gag G u acg Thr 385 tat Tyr ttg Leu at g Met cct Pr o gcc Al a 290 tgg Tr p ct c Leu ct g Leu at c SIle gt g Val 370 cag G n ct g Leu aaa Lys gca Al a gt g Val 275 cag G n aag Lys gat Asp gct Al a gt g Val 355 t cc Ser gac Asp t cc Ser ggt Gy tca Ser 260 ct g Leu gt g Val ttc Phe gac Asp gt g Val 340 gag G u tac Tyr ctt Leu aac Asn 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1350 taaagcgcgg gcctgctggt ggc <210> 290 <211> 409 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 290 let Ala Pro Val Thr y Leu Pro Val Thr Pro Tyr Ser n u Ala 1 5 10 Ser I e y Ala Ser Phe Pro Ala Val Asp Pro Asp Thr Lys Asp Ser 25 Ala Ala Tyr G y His u Ser G y Iet Arg G u Arg I I e Ser Asn Ala 40 Page 371 seqi i st car r ect ed. t xt v Ser Ser Ser Leu Thr Lys Ar g Val Val Val Lys I Ie 03 As nAsp (1u As p Le u G31y Thr Le u G31y 145 Ar g Pr o G31y As p Pr o 225 As p G31y ValI As p A a 305 Ar g Le u O31u O31u Thr 385 Tyr Hi s A a ValI Le u 115 Hi s ValI A a ValI As n 1 95 Le u As p Ly s A a ValI 275 O31n Ly s As p A a ValI 355 Ser As p Ser ValI vb t A a ValI Tr p Le u Ar g 1 65 O31u Ar g Le u Thr ValI 245 Ly s Le u G31y Tr p G31y 325 G31y I Ie As p Pr o Tyr 405 As p 70 O31u G31y Ly s G31y Thr 150 Thr As n Le u Le u A a 230 li1e ValI Thr Thr A a 310 A a I Ie Le u Ser As p 390 A a Pr o A a vb t O31n Ar g 135 A a I Ie As p A a Ser 215 Ly s A a Ser Ser ValI 295 Le u ValI Thr G31y As p 375 G31y Ser As n G31y A a A a 120 Ser A a As p Thr A a 200 As p Ph e G31y A a A a 280 Ph e Tyr O31u O31u Pr a 360 Thr vb t Ar g Ar g Ser Pr a 1 A a Ph e As p Ly s ValI 1 li1e ValI I Ie As p A a 265 A a Hi s A a A a I Ie 345 A a Le u O31n A a I Ie As p 90 Le u A a A a A a Le u 1 70 A a ValI As p Ser G31y 250 Ar g As n Pr a A a ValI 330 li1e G31y O31n Ar g As n Le u O31y A a Ar g O31y 1 Ar g Thr A a O31y O31u 235 O31y Le u I Ie Ly s As p 315 Thr O31y O31n Ser Pr a 395 I Ie ValI Ser O31y 125 O31y Ar g Le u O31y Le u 205 Ph e Ar g ValI Ser Pr a 285 As n A a O31y Ph e I Ie 365 ValI ValI As n Ser Ar g ValI Pr a Ar g A a 1 As n Ser Ly s O31y Thr 255 Ser Le u Le u Ly s Ly s 335 O31n Ar g vb t A a Page 372 seql i st cor r ect ed. t xt <210> 291 <211> 1419 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1396) <223> RXN02382 <400> 291 gagcagatgg ccaccgaggt cgatgtggtg gctggctact cgacactgtt gct t ct t aag ggtctgtata gtgggcaacc aggcct t t t a tggtgtgatc atg agt tca acg acc 11! lMt Ser Ser Thr Thr ct a Leu aaa Lys acc Thr gca Al a ggt Sy gat Asp ggc G y aac Asn at g Met gca Al a 150 gt g Val cgt Ar g gat Asp act Thr ccg Pr o agc Ser gca Al a tct Ser acc Thr at t SIle 120 t at Tyr aag Lys t cc Ser gag G u caa G n gct Al a aac Asn gaa G u ggc G y at t I e cca Pr o at g Met gcc Al a gga Gy acc Thr 170 cca Pr o cgc Ar g aac Asn at c SIle at c SIle gaa G u ggc G y ggt G y cag G n cct Pr o 140 gt a Val ct c Leu gaa G u gac Asp at c SIle ct g Leu 45 gaa G u gaa G u at c SIle gaa G u gt c Val 125 aac Asn gct Al a gt g Val acc Thr aat Asn gt c Val cgt Ar g gcc Al a t cc Ser gct Al a gt a Val 110 cgt Ar g gt c Val ttg Leu gaa G u gt g Val 190 cgg Ar g gat Asp gcg Al a gct Al a at t SIle ggt G y cgc Ar g cct Pr o gt c Val cgc Ar g 160 ct g Leu ttg Leu gaa G u gca Al a gat Asp gat Asp cgc Ar g cgc Ar g cat Hi s ggc Gy 130 gcc Al a t cc Ser gac Asp cct Pr o ct a Leu ttg Leu ct c Leu gaa G u gcc Al a gtt Val 100 at g Met at g let ggc Gy aca Thr ct c Leu 180 caa G n cgc gga tcc gtc caa gat ttg atc acc gca cgc ggc ctc gtt gac gtg Page 373 Arg G y Ser Val n Asp Leu II seql i st cor r ect ed. t xt e Thr Ala Arg y Leu 5 210 gt c Val gcg Al a 230 at c SIle ggc Gy ct c Leu ct c Leu gca Al a 310 tct Ser gat Asp gcg Al a gt c Val ggg Gy 390 ct g Leu 200 20 cca Pr o gt g Val gcc Al a acc Thr gcc Al a 280 gaa G u ggt Sy t ac Tyr gct Al a gcc Al a 360 gca Al a cag G n gca Al a ggc G y acc Thr gcc Al a 250 cgc Ar g ct c Leu gga Gy acc Thr t cc Ser 330 gca Al a caa G n gcg Al a ggc Gy gga G1y 410 ggc G y at t SIle 235 aag Lys tgc Cys agc Ser gt c Val gac Asp 315 tt c Phe cac Hi s aac Asn gt g Val at g bet 395 cca Pr o gca Al a 220 gaa G u ctt Leu agc Ser gac Asp aca Thr 300 gt g Val gat Asp at c SIle at t SIle at g Met 380 ggc Gy at g Met gga G y acc Thr gat Asp gt g Val t ca Ser 285 at t SIle gt g Val at c SIle gct Al a gaa G u 365 at a SIle gcg Al a gcc Al a ct a Leu ggc Gy cag G n tgc Cys 270 gac Asp cat Hi s gaa G u gct Al a aag Lys 350 acc Thr aac Asn gag G u ct g Leu at c SIle acc Thr gca Al a 255 aac Asn aag Lys gga G y gca Al a gt c Val 335 t ac Tyr gct Al a gca Al a at c SIle cca Pr o 415 aac Asn ggc G y 240 at c l Ie gct Al a ctt Leu cgg Ar g act Thr 320 gct Al a agc Ser cag G n t cc Ser ggc G y 400 gag G u gca gt g Al a Val 225 aac tgc Asn Cys gcc atg Al a Vet act gaa Thr G u gca gt c Al a Val 290 gtg gcc Val Ala 305 gaa act G u Thr gtg gtt Val Val acc aag Thr Lys cgc ttt Arg Phe 370 acc gcc Thr Al a 385 att tcc Ile Ser ctg acc Leu Thr Val Asp Val gt c acc ggt Val Thr G y cac ttc tac Hi s Phe Tyr 245 gt c at c aac Val Ile Asn 260 acc gcg ctt Thr Ala Leu 275 gtc cag gcg Val n Ala gaa ttg gaa G u Leu G u gac tgg gat Asp Trp Asp 325 gac ggt gt g Asp G y Val 340 cac acc gaa Hi s Thr G u 355 gca gat cgc Al a Asp Arg tac acc gat Tyr Thr Asp acc cag aaa Thr G n Lys 405 t cc acc aag Ser Thr Lys 420 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 tgg att ctg cag ggc aca gga caa att agg cct Trp lie Leu On Oy Thr Oy On lie Arg Pro 425 430 agc <210> 292 <211> 432 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 292 taagtttgaa gaagtaatca 1416 1419 Page 374 seqI i st car r ect ed. t xt I'bt Ser Ser Thr Thr Leu Thr Asp Asp O31n I Ie Ar g Asp 1 5 10 Asn O31u Ar g Thr I Ie Al a Ser As p Le u G31y Le u As p 145 O31y O31n Le u O31y Al a 225 As n Al a Thr Al a ValI 305 O31u ValI ValI ValI O31u I Ie Le u O31n ValI 115 ValI Ph e Ser ValI cys 1 95 ValI ValI Hi s ValI Thr 275 ValI (31u As p As p Al a G31y ValI Al a Le u Al a (31u G31y Le u Al a 1 65 O31u Thr ValI G31y Tyr 245 As n Le u Al a O31u As p 325 ValI Ly s Thr Al a G31y 70 As p G31y As n vb t Al a 150 ValI Ar g Ar g ValI Al a 230 li1e G31y Le u Le u Al a 310 Ser As p Al a G31y Ar g Ar g O31u Le u G31y ValI 135 Le u Hi s Ph e Gy le 215 Thr As p Ly s As p O31n 295 Ph e O31u (31y Thr Pr o 40 Ser Al a Ser Thr I Ie 120 Tyr Ly s Ser O31u Ser 200 Pr o ValI Al a Thr Al a 280 O31u O31y Tyr Al a Al a Ly s Al a As n Ar g As p 1 05 O31n O31u Ser As n Le u 1 85 ValI Ar g Pr o O31u Ar g 265 Al a Al a Al a Le u Le u 345 Al a As n O31u O31y I Ie 90 Pr o vb t Al a O31y Thr 1 70 Pr a O31n O31y Thr Al a 250 Ar g Le u O31y Thr Ser 330 Al a Ly s Al a I Ie vb t O31u ValI Ly s Ar g As n 1 Ly s Ar g As p Oy le 235 Ly s cys Ser ValI As p 315 Ph e Hi s I Ie Le u O31u O31u I Ie O31u ValI 125 As n Al a ValI Thr I Ie 205 O31y Thr As p ValI Ser 285 li1e ValI I Ie Al a ValI Ar g Al a Ser Al a ValI 110 Ar g ValI Le u O31u ValI 1 Thr Le u O31y O31n cys 270 As p Hi s O31u Al a Ly s 350 Pr a Al a As n vb t O31y Le u ValI Thr Le u I Ie 1 O31n Al a I Ie Thr Al a 255 As n Ly s O31y Al a ValI 335 Ty r Thr Lys His Thr cOu Ala IlieAla Thr On Asn Ilie Ou Thr Ala O1n 355 365 Page 375 seqI i st car r ect ed. t xt Arg Phe Ala Asp Arg Val Asp Ala Ala Ala Val ~Ibt I Ie Asn Ala Ser 370 375 380 Thr Ala Tyr Thr Asp (31y O31u O31n Tyr O31y bt O31y Ala O31u Ilie O31y 385 390 395 400 Ilie Ser Thr O31n Lys Leu Hi s Ala Ar g O31y Pr o I'bt Ala Leu Pr o O31u 405 410 415 Leu Thr Ser Thr Lys Tr p Ilie Leu O31n O31y Thr O31y O31n I Ie Ar g Pr o 420 425 430 <210O> 293 <21 1> 724 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 OIDS <222> (709) <223> FFXA02378 <400> 293 gagcagat gg ccaccgaggt cgat gt ggt g gct ggct act cgacact gt t gct t ct t aag ggt ct gt at a gt gggcaacc aggcctt t ta t ggt gtgatc ct a Le u aaa Ly s ac c Thr gca A a ggt G31y g at As p ggc G31y aac As n at g vb t g at As p act Thr ccg Pr o ag c Ser gca A a t ct Ser ac c Thr at t I Ie 120 t at Tyr c aa O31n gct A a aac As n g aa O31u O31y at t lIe cca Pr o at g vb t gcc A a cgc Ar g aac As n at c I Ie at c I Ie g aa O31u O31y ggt O31y c ag O31n cct Pr o g ac As p at c I Ie ct g Le u 45 g aa O31u g aa O31u at c I Ie g aa O31u gt c ValI 125 aac As n aat gag As n (1u 15 gt c ccg Val Pr o cgt gcg Ar g Ala gcc aac A a As n t cc at g Ser I'b t gct ggc A a 03 y 95 gt a ct g Val Leu 110 cgt gt g Ar g Val gt c acc Val Thr cgg Ar g g at As p gcg A a gct A a at t I Ie 80 ggt O31y cgc Ar g cct Pr o at g vb t 1 ac c Thr at t I Ie gca A a t cc Ser g at As p tt g Le u gga O31y tt g Le u g aa O31u gca A a g at As p g at As p cgc Ar g cgc Ar g cat Hi s ggc O31y 130 ct a Le u tt g Le u ct c Le u g aa O31u gcc A a gt t ValI 1 00 at g vb t at g vb t agt t ca acg Ser Ser Thr ac c Thr gct A a ggc O31y gt t ValI gcg A a ct t Le u gct A a g aa O31u ggc O31y gt c gac gcc t tc ggc ct g Val Asp Ala Phe O31y Leu Page 376 gca Al a 1 50 gt g ValI cgt Ar g gcg Al a aag Ly s t cc Ser gag (31u ccg Pr o 200 gga G31y ac c Thr 1 70 cca Pr o aag Ly s seqI i st car r ect ed. t xt 140 145 gt a gct t tg ct g cgc ggt t cc t cc aca gct Val Al a Leu Leu Ar g (31y Ser Ser Thr Al a 160 165 ct c gt g gaa at c ct g cag gac gt a ct c gag Leu Val O31u I Ie Leu O31n As p Val Leu O31u 175 180 gaa acc gt g cag t tg ct g ct t gcc aaa ccc O31u Thr Val O31n Leu Leu Leu Al a Lys Pr o 190 195 t gat caccgg acgcg <210O> 294 <21 1> 203 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut anri cumn <400> 294 [Vbt Ser Ser Thr 03 u Val I e Al a Val Al a As p 03 u Ser As p I I e Asp Arg Leu Leu Ar g O31n O3 y Hi s Val 115 Leu 03 y Val 130 Asp Al a Phe 145 O3 y Ser Ser O3 n Asp Val Leu Leu Al a 1 95 <210> 295 <211> 623 <212> DNA Thr Thr Leu Al a Leu 03 y Leu Val 03 u Al a Al a Leu Val Al a 1 00 [vb t (1u [Vb t (1y O3 y Leu Thr Al a 1 65 Leu 03 u 1 80 Leu Thr Asp Asp G n 10 Lys Al aThr Al aAl a 25 Thr Gy Pro Lys Asn 40 Al a Ar g Ser Al a 0Ou 0Gy Ar g Al a As n 0Gy 70 Asp 0Ou Se r Ar g I Ie 90 0Gy Leu Thr Asp Pr o 1 05 As n 0 y I I e 0 n I'b t 120 I'b t Val Ty r 0Ou Al a 135 Al a Leu Lys Ser 0 y 150 Val Hi s Ser As n Thr 1 70 Ar g Phe 0Ou Leu Pr o 1 85 I Ie Ly s Al a I Ie vb t O31u ValI Ly s Ar g As n 1 Ly s Ar g As p I Ie Le u O31u O31u I Ie O31u ValI 125 As n Al a ValI Th r As n ValI Ar g Al a Ser Al a ValI 110 Ar g ValI Le u O31u ValI 1 G u Pr o Al a As n vb t 0Gy Le u ValI Th r Le u I Ie 1 0On Lys Pro Ala Asp Pro Ser Lys Ilie 200 Page 377 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA02382 <400> 295 ccc Pr o 1 gaa G u cgc Ar g gcc Al a gca Al a gca Al a ct g Leu ct g Leu acc Thr gca Al a 145 t ac Tyr cgt Ar g cag Gn at t SIle aag Lys tgc Cys agc Ser gt c Val gac Asp ttc Phe cac Hi s 115 aac Asn gt g Val at g Met cca Pr o aca Thr 195 acc Thr 5 gat Asp gt g Val t ca Ser at t SIle gt g Val at c SIle gct Al a gaa G u at a SIle gcg Al a 165 gcc Al a ggc Gy cag G n tgc Cys gac Asp cat Hi s 70 gaa G u gct Al a aag Lys acc Thr aac Asn 150 gag G u ct g Leu acc Thr gca Al a aac Asn aag Lys gga Gy gca Al a gt c Val t ac Tyr gct Al a 135 gca Al a at c SIle cca Pr o aac Asn gcc Al a act Thr gca Al a gt g Val gaa G u gt g Val 105 acc Thr cgc Ar g acc Thr at t SIle ct g Leu 185 tgc Cys 10 at g Met gaa G u gt c Val gcc Al a act Thr 90 gtt Val aag Lys ttt Phe gcc Al a t cc Ser 170 acc Thr cac Hi s gt c Val acc Thr gt c Val gaa Gu 75 gac Asp gac Asp cac Hi s gca Al a t ac Tyr 155 acc Thr t cc Ser t ac Tyr aac Asn ctt Leu gcg Al a gaa Gu gat Asp gt g Val gaa G u 125 cgc Ar g gat Asp aaa Lys aag Lys at c SIle ggc Gy ct c Leu ct c Leu gca Al a tct Ser gat Asp 110 gcg Al a gt c Val ggg Sy ct g Leu tgg Tr p 190 gat Asp aag Lys gac Asp cag G n ttc Phe gag G u gga Gy at c l Ie gat Asp gag G u cat Hi s 175 at t SIle gcc Al a acc Thr gcc Al a gaa G u ggt Gy t ac Tyr gct Al a gcc Al a gca Al a cag G n 160 gca Al a ctg Leu gga caa att agg Gy On lie Arg taagtttgaa gaagtaatca agc <210> 296 <211> 200 <212> PRT <213> Corynebacteri um gl utam cum <400> 296 Pro Thr IIe G u Thr G y Thr Gy Asn Cys His Phe Tyr IIe Asp Ala Page 378 seql i st cor r ect ed. t xt Asp 3 n Al a Ile G u Ar g Al a Al a Al a Leu Leu Thr Al a 145 Tyr Ar g G n Lys Cys Ser Val Asp Phe Hi s 115 Asn Val bet Pr o Thr 195 Val Ser SIle Val I le Al a G u SIle Al a 165 Al a G n Asn Lys Gy Al a Val Tyr Al a 135 Al a l Ie Pr o Ar g Al a 40 Leu Ar g Thr Al a Ser 120 G n Ser Gy G u Pr o 200 10 Al a Vet Thr G u Al a Val Val Ala G u Thr 90 Val Val 105 Thr Lys Arg Phe Thr Al a I e Ser 170 Leu Thr 185 Val I e Asn Gy Thr Ala Leu Leu Val G n Ala Leu G u Leu G u Al a 75 Asp Trp Asp Ser Asp y Val Asp 110 His Thr G u Al a 125 Ala Asp Arg Val 140 Tyr Thr Asp G y 155 Thr G n Lys Leu Ser Thr Lys Trp 190 Lys Thr Asp Al a G n G u Phe G y G u Tyr G y Al a I e Al a Asp Al a G u G n 160 Hi s Al a 175 II e Leu <210> 297 <211> 933 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(910) <223> RXA02499 <400> 297 cctggtcatg acgt t cgggc cgctggcaat cgtcatgtaa ctgctgcaaa acaggggt gg t t agtggcag t gt gggaacc gta atc ggc ggc gga caa atc ggc gag Val I le y G y G y Gn le G y u gcg gcc aac atg aat cca caa aat att Ala Ala Asn IVet Asn Pro n Asn lie 30 gag cgc ggc caa gag ctg cgt gac cgc G u Arg G y On G u Leu Arg Asp Arg 45 gct tta Al a Leu 15 cgc gtc Arg Val t ac ggc Tyr G y Page 379 tttgtcgttt tgggcccccg atg aca aca att gct IVet Thr Thr Ile Ala 1 gtc tca ggt ttg atc Val Ser y Leu Ile acc aac cgt tcg gaa Thr Asn Arg Ser G u at c ctc aac atg acg Ile Leu Asn IVet Thr gat aat tcc caa gcc gca Asp Asn Ser O n Al aAl a aag Ly s g at As p ag c Ser cgc Ar g gt c ValI g ac As p 1 50 at c I Ie ct t Le u cgc Ar g ac c Thr gt t ValI 230 g aa (31u aaa Ly s aac As n gct Al a at g vb t 120 aaa Ly s tt g Le u gca Al a ac c Thr ac c Thr 200 at g vb t t ca Ser gga G31y at c I Ie gca Al a at g vb t aac As n cgc Ar g ac c Thr ac c Thr 1 70 gcg Al a aaa Ly s g aa O31u gca Al a cga Ar g 250 gt c ValI 75 caa O31n gaa G31u act Thr t ac Tyr gt t ValI 155 gcg Al a ct c Le u aag Ly s acc Thr ggc 235 ggc G31y g ac As p g aa G31u agt Ser g aa G31u cca Pr a gt t ValI 140 gga (31y at g vb t at t I Ie ct c Le u ggc G31y 220 ac c Th r gct Al a seql i st car r ect ed. t xt gaa gcc gac gt g gt g t tc ct g t gc gt g G31u Al a Asp Val Val Phe Leu Cys Val gt g ValI gt t ValI ag c Ser at g vb t 125 g ac As p g ac As p t cc Ser gag G31u gct Al a 205 aaa Ly s ac c Th r tt c Ph e ct c Le u gt g ValI gcc Al a 110 ct c Le u gcg Al a gt c ValI gga G31y gca Al a 1 90 gt g ValI g aa G31u gt c ValI t ac Ty r t cc Ser gt c ValI 95 t ct Ser gt g ValI g aa G31u ct c Le u t cc Ser 1 75 gga G31y gcc Al a ccc Pr a gca Al a cgc Ar g 255 g aa G31u 80 ag c Ser gcg Al a ggc c ag O31n g aa (31u 1 60 t cc Ser gt t ValI t ca Ser t ca Ser gcc Al a 240 gca Al a ac c Th r gcc Al a ct c Le u ggc G31y 130 g aa O31u gcg Al a gca Al a ct a Le u g aa O31u 210 tt g Le u cga Ar g c aa O31n ggc (31y gca Al a ccc Pr a 115 at g vb t c aa O31n g aa (31u t ac Ty r ggc G31y 1 ggt G31y cgc Ar g g aa O31u gct Al a act Th r ggc G31y 1 00 gt c ValI t cg Ser gt c ValI t ca Ser ct g Le u 1 ct g Le u gct Al a gca Al a ct c Le u t gc Cys 260 tt g Le u at c I Ie gt g ValI act Th r aag Ly s g ac As p 1 tt c Ph e ccc Pr a gca Al a ggc G31y g aa O31u 245 gcc Al a gac cga t ct gaa gaa ct c gga aag cgc t agaaaccgt t at t t ccccg Asp Ar g Ser O31u O31u Leu G31y Lys Ar g 265 270 tt a <210O> 298 <21 1> 270 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 298 I'bt Thr Thr IleAlaVal IleOy OyOy On Ile OyOu Ala Leu 1 5 10 Page 380 seqI i st car r ect ed. t xt Val Ser Oy Leu Ilie Ala Ala Asn ~Ibt Asn Pro On Asn I Ie Ar g Val Thr I Ie ValI li1e vb t G31y Ly s Le u 145 ValI Pr o As n Ph e (31u 225 li1e A a Ar g As n Le u G31y A a Pr o 115 vb t O31n O31u Tyr G31y 1 95 G31y Ar g O31u A a O31u Thr ValI Le u li1e ValI Thr Ly s As p 1 65 Ph e Pr o A a G31y O31u 245 A a Ar g As n Pr o As n I Ie ValI Thr 135 Le u As p ValI A a vb t 215 Ser Ser Ar g O31n O31n Ph e Ser A a 1 05 Pr a G31y Ser ValI (31u 1 85 A a Ly s Pr a I Ie (31u 265 (31u A a I Ie A a 90 vb t As n Ar g Thr Thr 1 70 A a Ly s (31u A a Ar g 250 a u Le u A a ValI O31n O31u Thr Tyr ValI 1 A a Le u Ly s Thr O31y 235 O31y Le u Ar g As p O31u Ser O31u Pr a ValI 140 O31y vb t I Ie Le u O31y 220 Thr A a (31y As p O31u ValI ValI Ser vb t 125 As p As p Ser O31u A a 205 Ly s Thr Ph e Ly s Ar g A a Le u ValI A a 110 Le u A a ValI O31y A a 1 ValI O31u ValI Tyr Ar g 270 Tyr As p Ser ValI Ser ValI O31u Le u Ser 1 O31y A a Pr a A a Ar g 255 <210O> 299 <21 1> 1296 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1273) <223> RXS02157 <400> 299 gggt ggaat t ggcacgat gg t gct gccgga t gt tt t tgat cgggagaat t at cct gaagg caccgtt t tt agaaaagacg acaaggat gg ggaact gt aa atg agc acg ctg gaa 115 I'b t Ser Thr Leu 03 u 1 act t gg cca cag gt c at t at t aat acg t ac ggc acc cca cca gtt gag 163 Thr Trp Pro On Val Ilie lieAsn Thr Tyr Oy Thr Pr o Pr o Val O31u Page 381 seql i st cor r ect ed. t xt 15 ctg gtg tcc ggc aag ggc gca acc gtc act gat gac cag ggc aat gtc 211 Leu Val Ser y Lys G y Al a Thr Val Thr Asp Asp G n y Asn Val 30 tac atc gac ttg ctc gcg ggc atc gca gtc aac gcg ttg ggc cac gcc 259 Tyr IIe Asp Leu Leu Ala G y Ile Ala Val Asn Ala Leu G y His Ala 45 cac ccg gcg atc atc gag gcg gtc acc aac cag atc ggc caa ctt ggt 307 His Pro Al a Ie Il e G u Ala Val Thr Asn n I Ile G y G n Leu G y 60 cac gtc tca aac ttg ttc gca tcc agg ccc gtc gtc gag gtc gcc gag 355 His Val Ser Asn Leu Phe Ala Ser Arg Pro Val Val G u Val Ala G u 75 80 gag ctc atc aag cgt ttt tcg ctt gac gac gcc acc ctc gcc gcg caa 403 G u Leu Ile Lys Arg Phe Ser Leu Asp Asp Ala Thr Leu Ala Ala G n 95 100 acc cgg gtt ttc ttc tgc aac tcg ggc gcc gaa gca aac gag gct gct 451 Thr Arg Val Phe Phe Cys Asn Ser Gy Ala G u Ala Asn G u Ala Ala 105 110 115 ttc aag att gca cgc ttg act ggt cgt tcc cgg att ctg gct gca gtt 499 Phe Lys lie Ala Arg Leu Thr y Arg Ser Arg lie Leu Ala Ala Val 120 125 130 cat ggt ttc cac ggc cgc acc atg ggt tcc ctc gcg ctg act ggc cag 547 His G y Phe His G y Arg Thr IVet G y Ser Leu Ala Leu Thr G y G n 135 140 145 cca gac aag cgt gaa gcg ttc ctg cca atg cca agc ggt gtg gag ttc 595 Pro Asp Lys Arg G u Al a Phe Leu Pro IVet Pro Ser y Val G u Phe 150 155 160 165 tac cct tac ggc gac acc gat tac ttg cgc aaa atg gta gaa acc aac 643 Tyr Pro Tyr y Asp Thr Asp Tyr Leu Arg Lys IVet Val G u Thr Asn 170 175 180 cca acg gat gtg gct gct atc ttc ctc gag cca atc cag ggt gaa acg 691 Pro Thr Asp Val Ala Al a lle Phe Leu G u Pro lie n Gy G u Thr 185 190 195 ggc gtt gtt cca gca cct gaa gga ttc ctc aag gca gtg cgc gag ctg 739 Sy Val Val Pro Ala Pro G u y Phe Leu Lys Ala Val Arg G u Leu 200 205 210 tgc gat gag tac ggc atc ttg atg atc acc gat gaa gtc cag act ggc 787 Cys Asp u Tyr Gy Ile Leu IVet lie Thr Asp u Val n Thr y 215 220 225 gtt ggc cgt acc ggc gat ttc ttt gca cat cag cac gat ggc gtt gtt 835 Val G y Arg Thr G y Asp Phe Phe Ala His G n His Asp G y Val Val 230 235 240 245 ccc gat gtg gtg acc atg gcc aag gga ctt ggc ggc ggt ctt ccc atc 883 Pro Asp Val Val Thr IVet Ala Lys y Leu Gy Gy y Leu Pro Ile 250 255 260 ggt gct tgt ttg gcc act ggc cgt gca gct gaa ttg atg acc cca ggc 931 G y Al a Cys Leu Al a Thr G y Arg Al a Al a G u Leu IVbt Thr Pro G y 265 270 275 aag cac ggc acc act ttc ggt ggc aac cca gtt gct tgt gca gct gcc 979 Lys His G y Thr Thr Phe G y G y Asn Pro Val Ala Cys Ala Ala Ala Page 382 seqI i st car r ect ed. t xt 280 285 290 aag gca gt g ct g t ct gt t gt c gat gac gct t tc t gc gca Lys Al a Val Leu Ser Val Val Asp Asp Ala Phe Cys Al a 295 300 305 cgc aag ggc gag ct g t tc aag gaa ct t ct t gcc aag gt t Ar g Lys G31y O31u Leu Phe Lys O31u Leu Leu Al a Lys Val 310 315 320 gt agac gt ccgt ggc agg ggct t gat gt t gggc gt ggt g Val Asp Val Arg G31y Arg G31y Leu [Vb t Leu G31y Val Val 330 335 gac gt c gca aag caa gct gt t ct t gat ggt t tt aag cac Asp Val Al a Lys O31n Al a Val Leu Asp G31y Phe Lys Hi s 345 350 t tg aat gca ccg gcg gac aac at t at c cgt t tg acc ccg Leu Asn Al a Pr o Al a Asp Asn Ilie Ilie Ar g Leu Thr Pr o 360 365 370 at c acc gac gaa gaa at c gca gac gca gt c aag gct at t Ile Thr Asp (1u (1u Ile Al aAsp Ala Val Lys Al a Ilie 375 380 385 at c gca t aaaggact c aaact tat ga ct t Ile Al a 390 <210> 300 <211> 391 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn g aa (31u g ac As p ct g Le u ggC G31y 355 ccg Pr o gcc Al a gt t ValI G31y gag (31u 340 gt t ValI ct g Le u gag a u 1 027 1 075 1123 1171 1219 1267 1296 <400> 300 I'bt Ser Thr Thr Pr o Pr o Asp O31n G31y Al a Leu 03 y li1e 31y O31n Val O31u Val Thr Leu Al a Al a As n 03 u 115 1 1 e Leu Al a 130 Al a Leu Thr 145 Leu 03 u Val O31u As n Val Hi s Al a Leu 03 y Al a 03 u Al a 03 n 1 00 Al a Al a Al a Val (31y (1n Tr p Pr o O31n ValI I Ie Pr o 55 ValI Le u Ar g Ly s G31y 135 As p G31y 25 Le u I Ie As n Ly s Ph e 1 05 Al a Hi s Ar g I Ie G31y Al a (31u Ph e 75 Ph e Cys Le u Ar g Al a 1 55 I Ie Al a G31y Al a Al a Ser As n Thr Thr 140 Ph e As n Thr I Ie ValI Ser Le u Ser G31y 125 vb t Le u Thr ValI Al a Thr Ar g As p G31y 110 Ar g G31y Pr o Tyr Thr ValI As n Pr a As p Al a Ser Ser vb t G31y As p As n O31n ValI Al a O31u Ar g Le u Pr a 1 Ser O31y Val O31u Phe Tyr Pro Tyr O31y Asp Thr Asp Tyr Leu Ar g Lys Page 383 seqI i st car r ect ed. t xt 1 65 Asn Pro Thr Asp 1 70 Al aAl a Il e Phe vb t I Ie Al a (31u 225 Hi s (31y Le u Al a cys 305 Ly s ValI Ly s Thr Al a 385 (31u G31y 1 95 Ar g O31n G31y Le u Thr 275 Al a (31u As p Le u (31y 355 Pr o Al a Thr Le u (31y ValI 245 li1e (31y Al a Al a ValI 325 Ar g I Ie ValI Thr ValI As p 215 (31y As p Al a Hi s Al a 295 Ly s As p ValI As n Thr 375 Al a ValI 200 (31u Ar g ValI cys (31y 280 ValI (31y ValI Al a Al a 360 ValI 1 85 Pr o Tyr Thr ValI Le u 265 Thr Le u (31u Ar g Ly s 345 Pr o Al a (31y (31y Thr 250 Al a Thr Ser Le u (31y 330 O31n Al a Pr o I Ie As p 235 vb t Thr Ph e ValI Ph e 315 Ar g Al a As p G31u Le u 220 Ph e Al a (31y (31y ValI 300 Ly s (31y ValI As n G31y 205 vb t Ph e Ly s Ar g (31y 285 As p O31u Le u Le u I Ie 365 1 Leu O31u Pr o 1 Phe Leu Lys Il e Thr Asp Al a Hi s 03 n 240 03 y Leu 03 y 255 Al a Al a 03 u 270 Asn Pr o Val Asp Al a Phe Leu Leu Al a 320 I'b t Leu G31y 335 Asp 03 y Phe 350 Ilie Ar g Leu Asp Gu Gu Ile Ala Asp Ala Val Lys 380 <210O> 301 <21 1> 1269 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1246) <223> RXS02262 <400> 301 gcaccaattt cggacctgaa atccccgagg aaaccgtgcc cgacgccgtg caggtgggcg tcgataagca aaaaatcgct gatactcgaa aggcctcaaa atg acc gca acc tac 115 I'b t Thr Al a Th r Tyr 1 acc act gaa acc gcc at c aat t tc t tg t tc t tg agc gaa ccg gac at g 163 Thr Thr Gu Thr Ala Ilie Asn Phe Leu Phe Leu Ser G31u Pr o Asp I'b t 15 atc gcg gcc gga gtc aaa gac gtc gcg caa tgc gtc gat gtc atg gag 211 Ilie Ala Ala Gy Val Lys Asp Val Al a O31n Cys Val Asp Val I'b t G31u Page 384 seql i st cor r ect ed. t xt gaa G u aac Asn ttt Phe ccc Pr o gga Gy acc Thr t cc Ser ggc Gy 150 gga Gy cgc Ar g at c SIle gt g Val gt c Val 230 t ac Tyr gcc Al a gtt Val cca Pr o ct c Leu aac Asn ggc Gy t ac Tyr aac Asn gt c Val 120 aac Asn aag Lys ggt Gy gga Gy gcc Al a 200 gcc Al a gcc Al a aaa Lys ggt Gy gac Asp 280 gcc Al a ct c Leu cat Hi s ccc Pr o ggc Gy gaa G u aac Asn ct g Leu tta Leu at g let 170 acc Thr gca Al a gga Gy acc Thr gaa G u 250 ttc Phe at g Met caa G n tt g Leu ggc Gy aag Lys 75 ggg Gy aac Asn gac Asp t cc Ser gcg Al a 155 gcg Al a acc Thr aca Thr tct Ser acc Thr 235 tgg Tr p gac Asp ggg Gy ct a Leu gcg Al a gcg Al a gac Asp cga Ar g aag Lys acg Thr gcc Al a 140 gt c Val aaa Lys at c SIle tgg Tr p gaa G u 220 acg Thr ct c Leu gac Asp ct c Leu ct c Leu cag G n 45 at g let ggc Gy ttc Phe gcc Al a gt c Val 125 t ac Tyr gcc Al a acc Thr aag Lys gcg Al a 205 gaa G u gac Asp aag Lys gct Al a t ac Tyr 285 ggc Gy ggc Gy at c SIle ccc Pr o aaa Lys t ca Ser 110 acc Thr cgc Ar g gac Asp at c SIle at c SIle 190 ttg Leu gac Asp gcc Al a ccg Pr o t at Tyr 270 gaa G u at t SIle gac Asp acc Thr gac Asp aac Asn 95 ggc Gy ggt Gy acc Thr gcg Al a acc Thr 175 aag Lys gaa G u gt g Val gcc Al a ggc Gy 255 ttg Leu gcc Al a t ac Tyr ttc Phe cgc Ar g acc Thr ttg Leu gca Al a ggc Gy aca Thr 160 gaa G u gga Gy aaa Lys gt c Val ggc Gy 240 gca Al a ctt Leu tgg Tr p aaa Lys ccg Pr o cga Ar g ggc Gy cct Pr o ccg Pr o gcg Al a 145 acc Thr gcg Al a cgc Ar g ttc Phe aaa Lys 225 t cc Ser ttg Leu gac Asp gca Al a at g Met gaa G u ttc Phe gt g Val cgc Ar g aag Lys 130 gtt Val ttg Leu tgc Cys agc Ser ccc Pr o 210 gac Asp t cc Ser ct g Leu gac Asp gaa G u 290 gcc Al a aac Asn at g Met aag Lys tcg Ser 115 gcc Al a ccc Pr o gct Al a at c SIle gaa G u 195 gag G u gcc Al a gcc Al a ct g Leu gcc Al a 275 gaa G u ggt Gy cca Pr o gcg Al a tgg Tr p 100 at c SIe at c SIe ggc Gy gt c Val gca Al a 180 cgc Ar g at c SIe gac Asp ttc Phe ctt Leu 260 cgc Ar g t ac Tyr 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 cca gga Pro G y acc cac tgg tac gac Thr His Trp Tyr Asp Page 385 295 300 ct g gcg ct g caa gga aaa ct C Leu Al a Leu G3 n G3 y Lys Leu 310 315 gat at c t gc t cc ggc aag ct a Asp Il e Cys Ser G3 y Lys Leu 330 at c ct c t at t cc gt c ggc ggc Ilie Leu Tyr Ser Val G31y G31y 345 acc caa gt g t at gaa aac gcc Thr G3 n Val Tyr G3 u Asn Al a 360 aac ctg tgg gaa tca ccc gca Asn Leu Tr p a31u Ser Pr o Al a 375 380 aaa <210> 302 <211> 382 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ar seqI i st car r ect ed. t xt 305 gac ctt gca aag at t tcc cag at t Asp Leu Al a Lys I I e Ser a3 n I I e 320 ccc gga cgc acc aac gat gag gaa Pr o a3 y Ar g Thr Asn Asp a3 u a3 u 335 340 at g cca gt a gaa gac gt c gcc t gg I'b t Pr o Val a31u Asp Val Al a Tr p 350 355 ct g gaa aaa ggc gt c ggc acc aca Leu a31u Lys a31y Val a31y Thr Thr 365 370 ct g gct t gagagaaga aacaacaat g Leu Al a 1 075 1123 1171 1219 1266 1269 <400> 302 I'bt Thr A Ser a3 u P Val As p V Lys I'b t A Pro a31u A Ar g Phe N/ a31y Val L Pr o Ar g S Pr o Lys A 130 Al a Val P 145 Thr Leu A Al a Cys I I a Thr r o Asp a1 Ib t I a a31y sn Pr o bt Al a ys Tr p 1 00 er I Ie I a I Ie r o a31y I a Val I e Al a 1 80 Tyr vb t a31u Le u a31u vb t Tyr Hi s vb t ValI ValI 1 65 a31u Thr Thr a uThr Al a 10 Ile Al aAl a a y Val 25 a3 u Th r Leu Val Leu 40 Asn Ser Asn Ser Hi s 55 Ph e 31u a31y I'bt Pr o 70 Pr o Al a Ty r Leu a31y 90 a3 y Se r As n Al a a3 1 05 Thr Phe Val Leu As n 120 Ser Al a As n Leu Leu 135 a31y Val Lys Hi s Leu 150 a31y Pr o a31y Val Nib t 1 70 Ar g Pr o a31y I Ie Thr 1 85 I Ie Lys5 Le u a31y Lys5 75 a31y As n As p Ser Al a 1 55 Al a Thr As n As p Al a Al a As p Ar g Lys5 Thr Al a 140 ValI Lys5 I Ie Ph e ValI a31n vb t a31y Ph e Al a ValI 125 Tyr Al a Thr Lys5 Le u Al a a31y I Ie Pr o Ly s Ser 110 Thr Ar g As p I Ie I Ie 1 Ph e a31n As p Thr As p As n a31y a31y Thr Al a Thr 1 Ly s Le u Cys Tyr Ph e Ar g Thr Le u Al a a31y Th r 1 a31u a31y Arg Ser au Arg ay lieAsn Ala Phe Ala Thr Trp Ala Leu au Lys Page 386 seqI i st car r ect ed. t xt 0 205 Ph e Ly s 225 Ser Le u As p Al a Thr 305 li1e As n As p ValI O31u Al a Al a Le u Al a 275 O31u Tr p O31n G31u Al a 355 Thr Gu le Pr o 245 Pr o Le u G31y As p G31y 325 li1e Al a Le u Val Al a Val 215 1 e Al a Al a Phe Lys Lys Ala G31y Ar g 265 Val As p Ty r 280 03 n Al a Ty r 295 Al a Leu (3 n I I e Cys Ser Leu Ty r Ser 345 O31n Val Ty r 360 Leu Tr p (31u 375 O3 y Se r 03 u 220 Thr Thr Thr 235 O31u Tr p Leu 250 Phe As p As p I'b t G31y Leu O3 n Leu Leu 300 O3 y Lys Leu 315 O3 y Lys Leu 330 Val G31y (1y O3 u Asn Al a Ser Pr o Al a 380 31u As p As p Al a Lys Pr o Al a Ty r 270 Ty r 03 u 285 G31y I Ie Asp Leu Pro G31y I'b t Pr o 350 Leu 03 u 365 Leu Al a ValI Al a G31y 255 Le u Al a Pr o Al a Ar g 335 ValI Ly s ValI G31y 240 Al a Le u Tr p G31y Lys 320 Th r O31u G31y <210O> 303 <21 1> 1491 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (1468) <223> RXS02970 <400> 303 aaccgacaaa acagccgtt c acgt gct aaa gca t tat t taaag act tcat aat att t tgggga gt g tgct cggc t t gat ct agg gt gaggt gag ;aact ggt t tg gca t tg aag ggt Leu Al a Leu Lys 03 y 1 gaa t tc gga t ct gt g caa gca O31u Phe G31y Ser Val O31n Al a 15 aac gat cgc gcg cac gtt ttc Asn Asp Ar g Al a Hi s Val Phe agc ccc aaa gt a t gg gca gct Ser Pr o Lys Val Tr p Al a Al a gac ggc aac gcc ttc atc gac Asp (31 y Asn Al a Phe Il e Asp Page 387 t ac Tyr aaa Ly s c ac Hi s gcc Al a aac As n gag O31u t gg Tr p ggt (31y g ac As p aaa Lys5 gcg Al a ac g Thr g aa O31u gca Al a g ac As p t ac Tyr tt c Ph e tt c Ph e aaa Lys5 45 g ac As p at c I Ie g ac As p at c I Ie tt c Ph e seql i st cor r ect ed. t xt at g Met tta Leu ccg Pr o tcg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr t ca Ser cac Hi s at c SIle 230 gca Al a ct c Leu ct g Leu acc Thr gt g Val 310 ggc G y t cc Ser gag G u ttc Phe gcc Al a 120 gcc Al a aaa Lys at g Met cca Pr o ttt Phe 200 gaa G u ct g Leu t ac Tyr at c SIle gct Al a 280 gcc Al a acc Thr ct c Leu ctt Leu at c SIle aat Asn ggc G y gag Su ct g Leu ct c Leu 170 at c SIle acc Thr gt c Val cca Pr o aat Asn 250 gac Asp gag Su ggt Sy t ca Ser t ac Tyr 330 gtc tcg gca aac tta ggc Val Ser Ala Asn Leu y 75 80 cag cgc caa gca gcc cgg G n Arg G n Al a Al a Ar g 95 gat gtg cgc tct gat gtt Asp Val Arg Ser Asp Val 110 gaa ttc tcc cac gtg ttt G u Phe Ser His Val Phe 125 cac tcc atc cgc atg gct His Ser Ile Arg IVet Ala 140 tcc gca tac cgc agc tac Ser Ala Tyr Arg Ser Tyr 155 160 acc ggc gaa cac cgc cgc Thr G y G u His Arg Arg 175 tac cac ttc tgg gca cca Tyr His Phe Trp Ala Pro 190 acc caa gaa gaa gaa tgc Thr n G u G u G u Cys 205 atc gcg ttt gaa ggt gct ile Al a Phe G u G y Al a 220 gtg gtg gga tca tca gga Val Val y Ser Ser y 235 240 ggc gtg cgc gaa ctt tgc G y Val Arg G u Leu Cys 255 gaa gtc atg gtc gga ttc G u Val Met Val G y Phe 270 cat gct ggc gac gat ttc Hi s Al a G y Asp Asp Phe 285 gtt aac gca ggt tac gcc Val Asn Ala Gy Tyr Ala 300 atc cgc gat acc ttc gga Ile Arg Asp Thr Phe G y 315 320 tcc gga cac cca ctt gca Ser Gy His Pro Leu Ala 335 cac aac aac cct Hi s Asn Asn ttg acc aac Leu Thr Asn gct gca aag Al a Al a Lys 115 ttc acc aac Phe Thr Asn 130 cgc ctg cac Arg Leu Hi s 145 cac ggc gca Hi s y Al a ctg ggc aac Leu G y Asn ttc ctg cac Phe Leu Hi s 195 gaa cgc gca G u Arg Ala 210 ggc atg at c G y Iet lle 225 at c at c ct g Ile Ile Leu aac aag cac Asn Lys Hi s gga cgc acc G y Arg Thr 275 cag cca gac Sn Pro Asp 290 cca ct c ggt Pro Leu G y 305 t ca gag gca Ser G u Al a gt a gca ccc Val Ala Pro Pr o at c SIle 100 at c SIle ggc G y acc Thr acc Thr ccc Pr o 180 cac Hi s ct c Leu gca Al a cca Pr o ggc G y 260 gga G y at g Met ggc G y t ac Tyr gcc Al a 340 cga Ar g aac Asn gt g Val ggc G y gga G y gga G y 165 acc Thr t cc Ser aag Lys gcg Al a cca Pr o 245 at c SIe aaa Lys at c SIe at c SIe t cc Ser 325 aag Lys 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 Page 388 sec gca gcg ctg gag at t t ac gcg gaa g Al a Al a Leu G3 u I I e Tyr Al a G3 u C 345 3 cga ct t ggc gct gaa ctg at c gaa c Ar g Leu O31y Al a O31u Leu Ilie O31u F 360 365 gaa aac gta gcg atc gct gac gtg c O31u Asn Val Al a Ilie Al a Asp Val A 375 380 gt g gag t tc aat gca gac gcc act g Val O31u Phe Asn Al a Asp Al a Thr P 390 395 ttc aag gaa cgc ggc gtg tgg ccg a Phe Lys O31u Arg O31y Val Tr p Pr o 410 atc gcg ccg ccg ctg acc acc act g Ilie Al a Pr o Pr o Leu Thr Thr ThrA 425 4 gac gcg gtg gaa gct gca gcc caa g Asp Al a Val O31u Al a Al a Al a O31n P 440 445 gcg t tg t tc t aagtt t tct agat aacaag Al a Leu Phe 455 <210> 304 <211> 456 <212> PRT <213> Cor ynebact er i urn gi ut ani cumn <400> 304 Leu Al a Leu Lys 03 y Tyr Thr Asn F 1 5 G31y Se r Val O31n Al a Lys O31u O31u C Ar g Al a Hi s Val Phe Hi s Ser Trp S 40 Lys Val Tr p Al a Al a Al a 31u G31y S 55 As n Al a Phe Ilie As p 'b t G31y Ser C 70 Hi s Asn Asn Pr o Arg Leu Val O31u A Leu Thr Asn Ilie Asn Pr o Al a Phe C 100 1 Al a Al a Lys Ilie Val Ser I'b t Al a A 115 120 Phe Thr Asn 03 y 03 y Al a Asp Al a 1 130 135 Ar g Leu Hi s Thr G31y Ar g Asn Lys I lIi st cor r ect ed. t xt ga gag atc att cca Sy 03 u Ilie I Ie Pr o ~50 ct cgc ct t cgt gaa ~ro Ar g Leu Ar g O31u 370 gg ggc atc gga ttc ~rg G31y Ilie G31y Phe 385 icc at g gct gcc ggt Il a I'b t Al a Al a 03 y 400 .tg at c t cc ggc aac /bt Ilie Ser G31y Asn 415 at gac gaa t tg gt a osp Asp 03 u Leu Val .30 ct gt c gag ct g acc I a Val O31u Leu Thr 450 Igcc cgc Ar g 355 ct a Le u tt c Ph e gct Al a cga Ar g gca Al a 435 tt c Ph e gt a ValI gcg Al a t gg Tr p gca Al a tt c Ph e 420 ct g Le u gct Al a gct Al a g aa O31u gca Al a g aa O31u 405 c ac Hi s ct g Le u ggg G31y 1171 1219 1267 1315 1363 1411 1459 1491 ~he e r e r Il a ~r g Ile I e As p (1y 10 Lys Ar g Al a 03 n Thr Leu Leu Val lie O31n 90 As n As p G31y 1ul 03 u Hi s Leu Ser Page 389 (3 u Phe I I e 03 u Phe Al a As p Ty r Se r Ar g ValI Ph e Se r 140 Al a Ph e Lys5 As p Al a O31n Ar g Ser 125 li1e Tyr As n Ser As p Le u Al a As p ValI vb t Se r seqi i st car r ect ed. t xt 1 Thr G y Gu Hi s Hi s Le u Ph e G31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI I Ie Ar g G31y 385 Al a G31y Le u Le u Al a As n Hi s 1 95 Al a I Ie Le u Hi s Thr 275 As p G31y Al a Pr o Ar g 355 Le u Ph e Al a Ar g Al a 435 Ph e G31y 1 65 Thr Ser Ly s Al a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s Al a G31u Al a G31u 405 Hi s Le u Ser Al a [Vbt Thr Ser Hi s I Ie 230 Al a Le u Le u Thr ValI 310 G31y Al a Ar g G31u ValI 390 Ph e I Ie As p As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y Al a Le u As n 375 G31u Ly s Al a Al a Pr o Ph e 200 G31u Le u Tyr I Ie Al a 280 Al a Thr Le u Le u G31y 360 ValI Ph e G31u Pr o ValI 440 l'b t Leu 1 70 As p I I e 1 Al a Thr As p Val G31u Pr o Leu Asn 250 Al a As p 265 Ty r 03 u Lys 03 y 03 n Ser Thr Tyr 330 G31u I Ie 345 Al a 03 u Al a I I e As n Al a Ar g G31y 410 Pro Leu Ty r Th r I Ie ValI 235 G31y G31u Hi s ValI I Ie 315 Ser Ty r Le u Al a As p 395 ValI Th r Hi s O31n Al a 220 ValI ValI ValI Al a As n 300 Ar g G31y Al a I Ie As p 380 Al a Tr p Th r Ph e O31u 205 Ph e G31y Ar g G31y 285 Al a As p Hi s O31u (31u 365 ValI Th r Pr a Th r O31n 445 Ar g 1 Al a (31u G31y Ser Le u 255 G31y As p Ty r Ph e Le u 335 (31u Ar g G31y vb t I Ie 415 As p 03 u Al a Al a Al a Al a Va1(3 Ou Al a 0 y Al a Leu Phe 455 <210O> 305 <21 1> 1330 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CDS <222> (1330) Page 390 seq i st car r ect ed. t xt <223> FRXA01009 <400> 305 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttatttaaag acttcataat attttgggga gtgaactggt ttgatctagg gtgag ttg gca ttg aag Leu Ala Leu Lys t ac Tyr aaa Lys cac Hi s gcc Al a at g lvb t tta Leu ccg Pr o t cg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr t ca Ser cac Hi s at c SI e 230 aac Asn gag 3u t gg Tr p ggt 3y t cc Ser gag (31u ttc Phe gcc Al a 120 gcc Al a aaa Lys at g lvb t cca Pr o ttt Phe 200 gaa O31u ct g Leu gac Asp aaa Lys gcg Al a acg Thr ct t Leu at c SI e aat Asn ggc G31y gag O31u ct g Leu ct C Leu 170 at c SI e acc Thr gt c Val cca Pr o g aa (31u gca Al a gac Asp t ac Tyr t cg Ser cgc Ar g gt g Val ttc Phe t cc Ser 140 gca Al a ggc G31y cac Hi s caa O31n gcg Al a 220 gt g Val ttc Phe ttc Phe aaa Lys 45 gac Asp gca Al a caa O31n cgc Ar g t cc Ser 125 at c SI e t ac Ty r g aa (31u ttc Phe g aa O31u 205 ttt Phe gga (31y at c SI e gac Asp at c SI e ttc Phe aac Asn gca Al a t ct Ser 110 cac Hi s cgc Ar g cgc Ar g cac Hi s t gg Tr p 190 g aa (31u g aa (31u t ca Ser g aa O31u 15 aac Asn agc Ser gac Asp tta Leu gcc Al a 95 gat Asp gt g Val at g lvb t agc Ser cgc Ar g 175 gca Al a g aa O31u ggt G31y t ca Ser ttc Phe gat Asp ccc Pr o ggc G31y ggc G31y 80 cgg Ar g gt t Val ttt Phe gct Al a t ac Ty r 160 cgc Ar g cca Pr a t gc Cys gct Al a gga (31y 240 1 gga G31y cgc Ar g aaa Lys aac Asn cac Hi s ttg Leu gct Al a ttc Phe cgc Ar g 145 cac Hi s ct g Leu ttc Phe g aa O31u 99C G31y 225 at c SI e t ct Ser gcg Al a gt a Val gcc Al a aac Asn acc Thr gca Al a acc Thr 130 ct g Leu ggc G31y ggc G31y ct g Leu cgc Ar g 210 at g lvb t at c SI e gt g Val Cac Hi s t gg Tr p ttc Phe aac Asn aac Asn aag Lys 115 aac Asn cac Hi s gca Al a aac Asn cac Hi s 195 gca Al a at c SI e ct g Leu Caa O31n gt t Val gca Al a at c SI e cct Pr a at c SI e 100 at c SI e ggc G31y acc Thr acc Thr ccc Pr a 180 cac Hi s ct C Leu gca Al a cca Pr a ;gt g~ ggt G31y gca Al a ttc Phe gct Al a gac Asp cga Ar g aac Asn gt g Val ggc G31y gga G31y gga G31y 165 acc Thr t cc Ser aag Lys gcg Al a cca Pr a 245 ig 11! Page 391 seql i st cor r ect ed. t xt gca ggt tac tta Ala y Tyr Leu ct c Leu ct g Leu acc Thr gt g Val 310 ggc G y gca Al a cga Ar g gaa Gu gt g Val 390 ttc Phe at c SIle gct Al a 280 gcc Al a acc Thr ct c Leu ct g Leu ggc Gy 360 gt a Val ttc Phe gaa Gu aat ggc gtg cgc gaa ctt Asn G y Val Arg G u Leu 250 255 gac gaa gtc atg gtc gga Asp G u Val bet Val y 270 gag cat gct ggc gac gat G u His Ala G y Asp Asp 285 ggt gtt aac gca ggt tac Sy Val Asn Ala y Tyr 300 tca atc cgc gat acc ttc Ser Ile Arg Asp Thr Phe 315 tac tcc gga cac cca ctt Tyr Ser Gy His Pro Leu 330 335 att tac gcg gaa gga gag ile Tyr Ala G u G y G u 350 gaa ctg atc gaa cct cgc Su Leu lie u Pro Arg 365 atc gct gac gtg cgg ggc lie Ala Asp Val Arg y 380 gca gac gcc act gcc atg Al a Asp Al a Thr Al a IVet 395 ggc 410 tgc aac aag cac Cys Asn Lys Hi s ttc Phe ttc Phe gcc Al a gga G y 320 gca Al a at c SIle ctt Leu at c SIle gct Al a 400 gga G y cag G n cca Pr o 305 t ca Ser gt a Val at t SIle cgt Ar g gga 35y 385 cgc Ar g cca Pr o 290 ct c Leu gag G u gca Al a cca Pr o gaa G u 370 ttc Phe ggc G y 260 gga G y at g Met ggc G y t ac Tyr gcc Al a 340 gt a Val gcg Al a tgg Tr p at c SIe aaa Lys at c SIe at c SIe t cc Ser 325 aag Lys gct Al a gaa G u gca Al a gaa 405 883 931 979 1027 1075 1123 1171 1219 1267 1315 1330 gcc ggt gct gca Ala y Ala Al a <210> 306 <211> 410 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 306 Leu Al a Leu Lys G y Tyr Thr Asn Phe 1 5 Sy Ser Val n Ala Lys u u u Arg Ala His Val Phe His Ser Trp Ser 40 Lys Val Trp Ala Ala Al a G u y Ser 55 Asn Ala Phe Ile Asp IVet y Ser n 70 His Asn Asn Pro Arg Leu Val G u Ala Asp G y u 10 Lys Arg Ala Al a n Asp Thr Leu Tyr Leu Val Ser lie n Arg Page 392 Phe Phe Lys Asp Al a G n G u Asn Ser Asp Leu Al a seqi i st car r ect ed. t xt As n Pr o Ala Ph e Asn Asp Val Le u A a Ph e Ar g 145 Hi s Le u Ph e (31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI I Ie Ar g G31y 385 A a As n Ly s 115 As n Hi s A a As n Hi s 1 95 A a I Ie Le u Hi s Thr 275 As p G31y A a Pr o Ar g 355 Le u Ph e A a ValI G31y G31y G31y 1 65 Thr Ser Ly s A a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s A a G31u A a G31u 405 Ser A a Ar g 150 Ser Thr Ser Hi s I Ie 230 A a Le u Le u Thr ValI 310 G31y A a Ar g G31u ValI 390 Ph e As p 135 As n A a As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y A a Le u As n 375 G31u Ly s A a 120 A a Ly s vb t Pr o Ph e 200 G31u Le u Tyr I Ie A a 280 A a Thr Le u Le u G31y 360 ValI Ph e (31u G31y 1 05 Ar g le le vb t As p 1 85 A a As p O31u Le u A a 265 Tyr Ly s O31n Thr O31u 345 A a A a As n Ar g (31y (1u O3 u Hi s Leu Ser 1 55 Leu Thr 1 70 1 1 e Ty r Thr Thr Val I Ie Pr o Val 235 As n (1y 250 As p (1u O3 u Hi s O3 y Val Ser I Ie 315 Ty r Ser 330 1 1 e Ty r O3 u Leu I e A a A a As p 395 G31y 410 Ph e Ser 140 A a G31y Hi s O31n A a 220 ValI ValI ValI A a As n 300 Ar g G31y A a I Ie As p 380 Ar g Ser 110 Ser Hi s 125 li1e Ar g Ty r Ar g O3 u Hi s Phe Tr p 1 O31u (1u 205 Phe 03 u O3 y Ser Ar g O31u [Vb t Val 270 31y As p 285 A a 03 y Asp Thr Hi s Pr o O31u (1y 350 O31u Pr o 365 Val Ar g Asp Val ValI vb t Ser Ar g 1 A a O31u Ser Le u 255 G31y As p Ty r Ph e Le u 335 O31u Ar g G31y Ph e A a Ty r 1 Ar g Pr a cys A a G31y 240 cys Ph e Ph e A a G31y 320 A a I Ie Le u I Ie A a Thr A a l'bt A a 400 <210O> 307 <21 1> 3579 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 393 seql i st cor r ect ed. t xt <220> <221> CDS <222> (101)..(3556) <223> RXN00023 <400> 307 gggattatgt tgggggccac atctgaaaat agttgctggg gaaacggcac ccacaccgct gatcttgaag gagaaccacc atccatcaca cctacgatgc ct g Leu cgc Ar g gcg Al a acg Thr gca Al a t cc Ser gcg Al a at g Met gct Al a ct a Leu 150 gac Asp aag Lys acc Thr gcg Al a at t SIle tgg Tr p cgt Ar g ggt Gy cat Hi s ttg Leu ccg Pr o ggg Sy 120 gct Al a gaa G u gt g Val tct Ser aat Asn 200 gcg Al a ttg Leu gag G u gct Al a gt g Val ttg Leu cgg Ar g gtt Val at g let at t l Ie gt g Val ttg Leu 170 gt g Val gtt Val aaa Lys gct Al a tac Tyr gca Al a gat Asp t at Tyr 75 gcg Al a gt g Val at t SIle cag G n tt g Leu 155 tt g Leu gca Al a gt g Val ggc Gy acg Thr agc Ser gt g Val cga Ar g gag G u cag G n gtt Val gt g Val tcg Ser 140 ggc G y cgc Ar g tcg Ser gaa G u acg Thr 220 ct g Leu aaa Lys ttg Leu 45 gt g Val ttg Leu at t SIle cct Pr o gat Asp 125 gat Asp cga Ar g cgt Ar g cag G n cag G n 205 aag Lys tct Ser aag Lys cag G n gtt Val ggc Sy cag G n gcg Al a 110 gcc Al a ggg Sy aag Lys ccg Pr o at t SIle 190 ct g Leu ttc Phe gac Asp 15 gaa G u gat Asp cga Ar g aag Lys gcc Al a 95 gcg Al a cgc Ar g cac Hi s gag G u gat Asp 175 tcg Ser aca Thr at c SIle cag G n agc Ser cct Pr o act Thr at t I e 80 ggt Sy cgg Ar g gac Asp cgc Ar g gca Al a 160 gt g Val at g Met cct Pr o aac Asn at g Met 1 gct Al a gt g Val aat Asn gag Gu gct Al a tct Ser gct Al a aag Lys ct g Leu 145 gcg Al a gaa G u tgg Tr p tta Leu ct g Leu 225 gt g Val ccc Pr o ggt G y gat Asp ccg Pr o ttg Leu cga Ar g cag G n 130 aac Asn aag Lys t at Tyr ggt G y t at Tyr 210 gac Asp gac Asp aat Asn ttg Leu cgt Ar g tcg Ser gt g Val at c SIle 115 ttc Phe at c SIle cat Hi s gt g Val ttc Phe 195 at a SIle at g let aag Lys gcc Al a gaa G u gaa Gu acg Thr ggg Sy 100 cgg Ar g gcc Al a aat Asn ttg Leu t cc Ser 180 gaa ggu gag G u gag G u acg tcg at g Thr Ser IVet aat Asn gtg Val gat Asp ttc Phe gcg Al a at g let cgg Ar g cag G n aag Lys ttg Leu gat Asp 165 at c SIe gac Asp gcc Al a gaa G u Page 394 tac cgc gat ctg cgc ctg Tyr Arg Asp Leu Arg Leu 230 235 act at Thr lM seql i st cor r ect ed. t xt g gag gtg ttc aag cg t G u Val Phe Lys Ar g ctg ct c g Leu Leu aat Asn ctt Leu gag G u ggt Sy cca Pr o 310 cgc Ar g ct g Leu ct c Leu cag G n gag G u 390 gcc Al a aac Asn aag Lys ct c Leu aaa Lys 470 gac Asp gag G u gat Asp gt c Val 280 aat Asn gcc Al a ct c Leu gtt Val gt g Val 360 at g Met ct g Leu tct Ser at g Met gag G u 440 gac Asp acg Thr aac Asn cat Hi s 250 ct c Leu aca Thr cct Pr o gaa G u tgg Tr p 330 ggc Sy cgt Ar g t cc Ser t ac Tyr ct c Leu 410 gcc Al a agc Ser ccc Pr o ct c Leu gcg Al a 490 gaa G u ggt G y ggc G y at g bet cct Pr o 315 acg Thr cac Hi s ggg Sy gat Asp gt a Val 395 gt g Val at c SI e cgc Ar g gcg Al a gac Asp 475 ct c Leu ct a Leu gca Al a ggg G y gag G u 300 t cc Ser at g Met aac Asn gt a Val cag G n 380 cca Pr o cgc Ar g ttc Phe ttc Phe ccc Pr o 460 gcc Al a at c SIle gaa G u at c l Ie gcg Al a 285 cac Hi s aaa Lys cgc Ar g ctt Leu gcg Al a 365 gcg Al a gcc Al a cgc Ar g gac Asp cgc Ar g 445 ggc G y ccc Pr o caa G n gcc Al a cag G n 270 ggc G y gt c Val caa G n aaa Lys ttc Phe 350 gac Asp cgc Ar g gt g Val ct c Leu ct c Leu 430 gcc Al a ccc Pr o ct c Leu aac Asn gga Gy 255 gac Asp gtt Val cac Hi s gcc Al a gaa Gu 335 gac Asp cgt Ar g gcc Al a cgc Ar g gag G u 415 gac Asp t cc Ser aac Asn gt c Val caa G n 495 240 at t SIle ttg Leu aag Lys gcg Al a acc Thr 320 aac Asn at a SIle gt g Val gt c Val cca Pr o 400 gaa G u gcc Al a at a SIle cac Hi s cca Pr o 480 cag G n ttg Leu cag G n cgc Ar g 290 at c l Ie gcc Al a gag Gu ttc Phe ttc Phe 370 gtt Val gaa G u gcc Al a aac Asn gac Asp 450 caa G n at c SIle cag G n ttc Phe 275 ct g Leu acc Thr aat Asn ggc Gy gca Al a 355 gaa G u gac Asp ttc Phe gcg Al a ccg Pr o 435 ct c Leu gac Asp aac Asn gcg Al a 260 ggc G y gt c Val ggc G y t ac Tyr ct g Leu 340 cat Hi s at g Met gt c Val gac Asp agc Ser 420 t cc Ser gcc Al a cgc Ar g gag G u 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 tgg gcc aca aaa gcc Trp Ala Thr Lys Ala 500 Page 395 seql i st cor r ect ed. t xt gtc gcc acc gca gca gag ccc ggt Val Ala Thr Ala Ala u Pro Gy 505 gag G u gac Asp at t SIle 550 at c SIle ccg Pr o gcc Al a gt c Val gga Gy 630 ccc Pr o tgg Tr p aat Asn gt c Val cga Ar g 710 gt g Val aaa Lys ggc Gy ttg Leu 520 gct Al a t ac Tyr gt g Val at c SIle gaa Gu 600 gt g Val act Thr aag Lys gcc Al a gat Asp 680 ttg Leu gaa G u acc Thr gcc Al a ttg Leu 760 gag G u gcg Al a acc Thr gcc Al a 570 gaa G u gac Asp acc Thr gca Al a agc Ser 650 gtt Val gga Gy ggc Gy acc Thr tct Ser 730 ggc Gy ggc Gy ggg Gy tgg Tr p gcc Al a 555 gcg Al a gcc Al a gac Asp ccg Pr o gca Al a 635 caa G n ccc Pr o tgt Cys t cc Ser at c SIle 715 gcc Al a cat Hi s agc Ser gac Asp gca Al a 540 gag Gu gag Gu at t lile gt a Val ccc Pr o 620 ct c Leu cat Hi s cgc Ar g gcg Al a t cc Ser 700 aac Asn gac Asp gca Al a gt c Val gt c Val 525 gcg Al a at t SIle gt g Val gat Asp gac Asp 605 tgg Tr p gcg Al a tgc Cys gag G u ttg Leu 685 gag Gu ggc Gy cgc Ar g gga Gy t ac Tyr 765 tgg Tr p 510 gac Asp cgc Ar g ttg Leu ggc Gy ttc Phe 590 aat Asn aat Asn gcg Al a t cc Ser gtt Val 670 at c SIle acc Thr gaa G u gac Asp caa G n 750 gaa G u ttg gaa Leu 0 u aaa caa aca Lys G n Thr aag Lys cca Pr o cgc Ar g aaa Lys 575 gcc Al a gcg Al a ttc Phe ggc Gy gct Al a 655 ct g Leu agc Ser gcc Al a acc Thr ct c Leu 735 aaa Lys t ca Ser ct a Leu gcc Al a gt g Val 560 gct Al a cgc Ar g gaa Gu ccc Pr o gct Al a 640 gca Al a cat Hi s cat Hi s gcg Al a t cc Ser 720 gcc Al a tgt Cys gaa G u aac Asn 530 gaa G u cgc Ar g gaa G u t ac Tyr acc Thr 610 gcg Al a gt g Val gt c Val at t SIle cac Hi s 690 ttc Phe aaa Lys gcc Al a gca Al a ttc Phe 770 515 gat Asp cgc Ar g gga Gy caa G n gcg Al a 595 cca Pr o at c l Ie at c SIle gaa G u t ac Tyr 675 gt c Val t cc Ser aac Asn gac Asp gcc Al a 755 cgg Ar g aag ccg Lys Pro gtg cgc Val Arg gct gag Al a u cac ct g Hi s Leu 565 acc gac Thr Asp 580 cat ttg Hi s Leu gat cgc Asp Arg ccc gct Pro Al a cac aaa Hi s Lys 645 gcc ct c Al a Leu 660 cca gct Pro Al a gac cgc Asp Arg tcc tgg Ser Trp gcc at c Ala I e 725 ctg gt g Leu Val 740 t cc ct c Ser Leu aaa cag Lys G n 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 Page 396 seql i st cor r ect ed. t xt c at c at c aac taa cc ctg gta gac gcc gca Leu Val Asp Ala Ala tcc tca ct Ser Ser Leu Ile Val Asp 775 780 t cc Ser 790 cac Hi s cga Ar g ggc Sy gt c Val caa G n 870 gcc Al a t ac Tyr gga Sy ccc Pr o cac Hi s 950 gga Sy gca Al a acc Thr ct a Leu ct c Leu aaa Lys 840 ggc Sy ggc Gy gaa G u aac Asn tgg Tr p 920 t at Tyr cca Pr o gag G u aac Asn gga Gy acc Thr 810 gac Asp gga Sy at g Met gac Asp cgc Ar g 890 ggc Gy aaa Lys at g Met gaa G u gaa G u 970 gaa G u cca Pr o 795 ct c Leu acc Thr acc Thr aaa Lys tt c Phe 875 acc Thr at c SIle t cc Ser ct c Leu aca Thr 955 tgg Tr p tt c Phe ct c Leu gaa G u ggc Gy ttc Phe gcc Al a 860 gga Gy tgg Tr p ace Thr t cc Ser at g Met 940 aac Asn ct c Leu ggc Gy acc Thr gaa G u cga Ar g ttc Phe 845 acc Thr ct c Leu ctt Leu ggc Gy gt c Val 925 gga Gy ccg Pr o gaa G u agc Ser gaa G u gga Gy ct c Leu 830 cac Hi s gac Asp acc Thr gac Asp gcc Al a 910 ggc Gy acc Thr ct g Leu aaa Lys cca Pr o 990 ct c Leu gaa G u 815 tgg Tr p ct c Leu ct c Leu ggc Gy cac Hi s 895 at t l Ie ct c Leu tgg Tr p at c SIle gcc Al a 975 cgc Ar g ccc Pr o 800 agc Ser t ca Ser aca Thr aat Asn gga Gy 880 gt c Val gt c Val gga Gy gcc Al a agc Ser 960 aac Asn gac Asp Tr p 785 agc Ser tgg Tr p ccc Pr o gaa G u gaa G u 865 ct c Leu gat Asp caa G n t cc Ser gac Asp 945 aaa Lys gcc Al a ccc Pr o gat Asp ct g Leu ggc Gy gt a Val 850 gcc Al a caa G n gt c Val cgc Ar g aaa Lys 930 gcg Al a ct g Leu agc Ser t cc Ser Pr t acc aac ccc o Thr Asn Pro aaa Lys ct g Leu at c SIle 835 ttc Phe at c l Ie t cc Ser gga Gy caa G n 915 gcc Al a cca Pr o gat Asp gat Asp ggc G5y 995 ct c Leu aaa Lys 820 aaa Lys gga Gy gaa G u ct c Leu aac Asn 900 t cc Ser gga Gy agc Ser ct c Leu gaa G u 980 ct c Leu cac Hi s 805 ccc Pr o gaa G u cca Pr o ttc Phe gac Asp 885 gcc Al a ttc Phe gga Gy cac Hi s ccc Pr o 965 aca Thr gat Asp 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 3091 gta Val ct c Leu gcc Al a 1030 gaa Su gac Asp 1015 cgt Ar g gcc Al a 1000 gat Asp cgc Arg aac att ttc cgc tac cga cca gca gag gta Asn Ile Phe Arg Tyr Arg Pro Ala G u Val 1005 1010 tcc gcc aca ccc cga gaa act gcc cgc gca Ser Ala Thr Pro Arg G u Thr Ala Arg Ala 1020 1025 gcc ggg gtt act ccg cga gtt ctt caa aca Ala y Val Thr Pro Arg Val Leu G n Thr 1035 1040 Page 397 gt a Val ttg Leu cca Pr o cga 3139 Arg gca 3187 Al a gtt 3235 Val 1045 seql i st cor r ect ed. t xt tca gag caa gtc cgc gaa gta ttg tcc gct gct gga gtg agt gca gaa 3283 Ser G u G n Val Arg G u Val Leu Ser Ala Al a y Val Ser Ala G u 1050 1055 1060 aca gtc gat gat tcg gta ttt att tcc aac gtg ttg cgc ggc gaa tac 3331 Thr Val Asp Asp Ser Val Phe Ile Ser Asn Val Leu Arg G y G u Tyr 1065 1070 1075 gac gag aac tcc agc gtc cga gtc cgc tac ctg ggc aaa gtt agc gac 3379 Asp G u Asn Ser Ser Val Arg Val Arg Tyr Leu y Lys Val Ser Asp 1080 1085 1090 act gtc cgt gaa cgc cta tct gta cgg ccc gaa gtt gtt ctg ctt gac 3427 Thr Val Arg G u Arg Leu Ser Val Arg Pro G u Val Val Leu Leu Asp 1095 1100 1105 gat gca gta act gcc tcc ggt cga gtt gaa tta cgt tac tgg ctc aaa 3475 Asp Ala Val Thr Ala Ser y Arg Val G u Leu Arg Tyr Trp Leu Lys 1110 1115 1120 1125 gaa caa gca att tcc atg acg ttg cac cgt ttt gga aac cca gtt gcg 3523 G u G n Ala lie Ser IVet Thr Leu His Arg Phe y Asn Pro Val Ala 1130 1135 1140 gcc ttc cac gag ttg gcg gag gaa ctt aaa cgt tgatcgtttt gcgcatgggt 3576 Ala Phe His G u Leu Al a G u G u Leu Lys Arg 1145 1150 cgc 3579 <210> 308 <211> 1152 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 308 bet Thr Ser ?bet 1 Ala Val Asp Lys Val Pro Asn Al a Asn G y Leu G u G u Asp Arg G u Ala Pro Ser Thr Ser Leu Val G y 100 Ala Arg Ile Arg 115 Lys G n Phe Al a 130 Leu Asn Ile Asn 145 Asn 5 Val Asp Phe Al a Met Ar g G n Lys Leu Leu Pro lie u Ar g Al a Thr Al a 70 Ser Al a bet Al a Leu 150 Ser Lys Val Al a Phe Leu Val Val 135 Gy Tr p Ar g 40 Gy Hi s Leu Pr o Gy 120 Al a G u Leu 25 Leu Phe Al a Asp G n 105 Hi s G u Al a Leu 10 G u Al a Val Leu Ar g 90 Val bet SIle Val Al a Tyr Al a Asp Tyr Al a Val SIle G n Leu 155 Leu Lys Leu Val Leu l Ie Pr o Asp 125 Asp Ar g Ser Lys G n Val Gy G n Al a 110 Al a Ly Lys Asp G u Asp Ar g Lys Al a Al a Ar g Hi s G u Ala Lys His Leu Asp Asp Thr Val Arg Leu Leu Arg Arg Pro Asp Val Page 398 seql i st cor r ect ed. t xt 165 Ile Lys Val Ser 170 Val Ala Ser n G u Tr p Leu Leu 225 Lys Val Al a Val G n 305 Asp bet Al a G u Ser 385 G n Asn Asp Ser Thr 465 Phe Tr p Lys Val Phe 195 I le b t Leu G n Phe 275 Leu Thr Asn Gy Al a 355 G u Asp Phe Al a Pr o 435 Leu Asp Asn Thr Thr 515 Asp Al a G u Ser 245 Tyr Ar g Lys Tr p Lys 325 Ar g Leu Leu Gy Val 405 G u Phe Thr Ser Pr o 485 Al a Pr o Thr Al a Ty r 230 Asn Leu G u Gy Pr o 310 Ar g Leu Leu G n G u 390 Al a Asn Lys Leu Lys 470 Asp Val G u Val Ar g 215 Ar g Pr o Pr o Ar g Al a 295 Val Val Gy Ser Gy 375 Leu l Ie Phe Ar g SIle 455 G u Thr Al a Val Asn 200 Al a Asp G u Asp Val 280 Asn Al a Leu Val Val 360 vbt Leu Ser vbt G u 440 Asp Thr Asn Thr Leu 520 Ser 185 Ty r Pr o Leu Leu Al a 265 Asn Leu Thr Tyr Al a 345 G u Al a Leu Tyr Ser 425 G u Val Leu Pr o Al a 505 G u Val Lys Ar g Hi s 250 Leu Thr Pr o G u Tr p 330 Gy Ar g Ser Tyr Leu 410 Al a Ser Pr o Leu Al a 490 Al a Val Gy Leu 235 G u Gy Gy b t Pr o 315 Thr Hi s Gy Asp Val 395 Val l Ie Ar g Al a Asp 475 Leu G u n 205 Lys Mbet G u SIle Al a 285 Hi s Lys Ar g Leu Al a 365 Al a Al a Ar g Asp Ar g 445 Gy Pr o G n Sy 175 lie Ser bet 190 Leu Thr Pro Phe II e Asn G u Val Phe 240 Al a G y l e 255 G n Asp Leu 270 G y Val Lys Val His Ala G n Al a Thr 320 Lys G u Asn 335 Phe Asp II e 350 Asp Arg Val Ar g Al a Val Val Arg Pro 400 Leu G u G u 415 Leu Asp Al a 430 Ala Ser Ile Pro Asn Hi s Leu Val Pro 480 Asn Gn n 495 Trp Leu G u 510 G u y Asp Val Asp Lys Leu 525 Page 399 lieAsn Asp Val Arg Asp 530 seqi i st car r ect ed. t xt Ala Ala O31u Ala Tr p Ala A a Ar g Pr o Ala Ar g 545 Ar g ValI Tyr Ph e I Ie 625 G31y ValI cys O31u vb t 705 G31y ValI Ser Ar g Tr p 785 Ser Tr p Pr o O31u O31u 865 Ar g G31y O31n A a 595 Pr o I Ie I Ie (31u Tyr 675 ValI Ser As n As p A a 755 Ar g Thr Ly s Le u I Ie 835 Ph e I Ie (31u Le u 565 As p Le u Ar g A a Ly s 645 Le u A a Ar g Tr p I Ie 725 ValI Le u O31n Pr o Hi s 805 Pr o O31u Pr a Ph e As p 885 535 Le u Ser O31u Le u ValI 615 Ser Ser O31u Ar g I Ie 695 Pr a ValI Ser I Ie ValI 775 A a A a O31n ValI Le u 855 As n Tyr ValI I Ie O31u 600 ValI Thr Ly s A a As p 680 Le u O31u Thr A a Le u 760 As p Thr Le u Le u Ly s 840 G31y G31y Ly s Thr Ser 585 Le u ValI Ph e Pr a G31y 665 ValI Thr Le u Pr a Ph e 745 ValI A a ValI Thr As p 825 Pr a Le u As n Thr A a 570 O31u As p Thr A a Ser 650 ValI G31y G31y Thr Ser 730 G31y G31y A a G31y Thr 810 As p G31y vb t As p A a 555 A a A a As p Pr a A a 635 O31n Pr a cys Ser I Ie 715 A a Hi s Ser Ser Pr a 795 Le u Thr Thr Ly s Ph e 875 540 O31u O31u I Ie ValI Pr a 620 Le u Hi s Ar g A a Ser 700 As n As p A a ValI Ser 780 Le u O31u O31y Ph e A a 860 (31y I Ie ValI As p As p 605 Tr p A a cys O31u Le u 685 O31u O31y Ar g O31y Tyr 765 Le u Thr O31u Ar g Ph e 845 Thr Le u Ar g Ly s 575 A a A a Ph e O31y A a 655 Le u Ser A a Thr Le u 735 Ly s Ser ValI Le u O31u 815 Tr p Le u Le u (31y ValI 560 A a Ar g O31u Pr a A a 640 A a Hi s Hi s A a Ser 720 A a cys O31u As p Pr a 800 Ser Ser Thr As n O31y 880 Leu 03 n Ser Leu Al aAsp (3u Val Ar g Thr Tr p Leu Asp Hi s Val 890 895 Page 400 seql i st cor r ect ed. t xt Asp Val Gy Asn Ala Tyr Val Asn Arg Gy Ile Thr Gy Ala Ile Val 900 905 910 G n Arg G n Ser Phe G y y Trp Lys Lys Ser Ser Val y Leu y 915 920 925 Ser Lys Ala Gy Gy Pro Asn Tyr Val IVet Leu IVet y Thr Trp Ala 930 935 940 Asp Ala Pro Ser His His Ala Pro Arg G u Thr Asn Pro Leu lie Ser 945 950 955 960 Lys Leu Asp Leu Pro G y G u G u Leu G u Trp Leu G u Lys Ala Asn 965 970 975 Ala Ser Asp G u Thr Al a Trp Asn Thr G u Phe y Ser Pro Arg Asp 980 985 990 Pro Ser G y Leu Asp Val G u Ala Asn Ile Phe Arg Tyr Arg Pro Al a 995 1000 1005 G u Val Val Leu Arg Leu Asp Asp Ser Ala Thr Pro Arg G u Thr Ala 1010 1015 1020 Arg Ala Leu Leu Ala Ala Arg Arg Ala G y Val Thr Pro Arg Val Leu 1025 1030 1035 1040 G n Thr Pro y Val Ser G u G n Val Arg G u Val Leu Ser Ala Ala 1045 1050 1055 Sy Val Ser Ala G u Thr Val Asp Asp Ser Val Phe lie Ser Asn Val 1060 1065 1070 Leu Arg Gy G u Tyr Asp G u Asn Ser Ser Val Arg Val Arg Tyr Leu 1075 1080 1085 Sy Lys Val Ser Asp Thr Val Arg G u Arg Leu Ser Val Arg Pro G u 1090 1095 1100 Val Val Leu Leu Asp Asp Ala Val Thr Ala Ser y Arg Val G u Leu 1105 1110 1115 1120 Arg Tyr Trp Leu Lys G u G n Ala lie Ser IVet Thr Leu His Arg Phe 1125 1130 1135 Sy Asn Pro Val Ala Ala Phe His u Leu Ala G u G u Leu Lys Arg 1140 1145 1150 <210> 309 <211> 476 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA00023 <400> 309 cat ttt ccg ctc cga cca gca gag gta gta ctc cga ctc gac gat tcc 48 His Phe Pro Leu Arg Pro Ala u Val Val Leu Arg Leu Asp Asp Ser 1 5 10 gcc aca ccc cga gaa act gcc cgc gca ttg ttg gca gcc cgt cgc gcc 96 Ala Thr Pro Arg G u Thr Ala Arg Ala Leu Leu Ala Ala Arg Arg Ala 25 Page 401 seqIi ggg gt t act ccg cga gtt ct t caa aca G31y Val Thr Pr o Ar g Val Leu O31n Thr 40 cgc gaa gt a t tg t cc gct gct gga gt g Ar g O31u Val Leu Ser Al a Al a G31y Val 55 t cg gt a t tt at t t cc aac gt g t tg cgc Ser Val Phe I Ie Ser As n Val Leu Ar g 70 agc gt c cga gt c cgc t ac ct g ggc aaa Ser Val Ar g Val Ar g Tyr Leu G31y Lys cgc ct a t ct gt a cgg ccc gaa gt t gt t Ar g Leu Ser Val Ar g Pr o O31u Val Val 100 105 gcc t cc ggt cga gt t gaa t ta cgt t ac Al a Ser G31y Ar g Val O31u Leu Ar g Tyr 115 120 t cc at g acg t tg cac cgt t tt gga aac Ser I'b t Thr Leu Hi s Ar g Phe G31y Asn 130 135 t tg gcg gag gaa ct t aaa cgt t gat cgtlt Leu Al a O31u O31u Leu Lys Ar g 145 150 <210> 310 <211> 151 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm cumn <400> 310 His Phe Pro Leu Arg Pro Ala Ou Val 1 5 Al a Th r Pro Arg Ou Thr Ala Arg Ala 25 G31y ValI Thr Pr o Ar g Val Leu O31n Thr 40 Ar g O31u Val Leu Se r Al a Al a G31y Val 55 Ser Val Phe Ilie Ser Asn Val Leu Arg 70 Ser Val Ar g Val Ar g Tyr Leu G31y Lys Ar g Leu Ser Val Ar g Pr o O31u Val Val 100 105 Al a Ser G31y Ar g Val O31u Leu Ar g Tyr 115 120 Ser I'b t Thr Leu Hi s Ar g Phe G31y Asn 130 135 Leu Al a 31u O31u Leu Lys Ar g st cor r ect ed. t xt cca ggt gt t tca gag Pro O31y Val Ser O31u agt gca gaa aca gtc Ser Al a 03 u Thr Val ggc gaa t ac gac gag 03 y 03 u Tyr Asp 03 u 75 gt t agc gac act gt c Val Ser Asp Thr Val 90 ct g ct t gac gat gca Leu Leu Asp Asp Al a 110 tgg ctc aaa gaa caa Tr p Leu Lys O31u O31n 125 cca gt t gcg gcc tt c Pr o Val Al a Al a Phe 140 :tt gcgcat gggt cgc c aa O31n g at As p aac As n cgt Ar g gt a ValI gca Al a c ac Hi s As p Ar g O31n As p As n Ar g ValI Al a Hi s gt c ValI g at As p t cc Ser g aa O31u act Thr at t I Ie gag O31u Le u Le u O31y Al a O31u Ser Le u Le u ValI Ar g Al a ValI O31u Tyr As p As p Ly s Al a 140 Le u Al a Ser Thr As p Thr As p O31u 125 Al a As p Ar g O31u ValI O31u ValI Al a 110 O31n Ph e Page 402 seqI i st car r ect ed. t xt 145 150 <210O> 311 <21 1> 3124 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (3124) <223> FRXA02284 <220> <221> misc-f eat ur e <222> 641 647 <223> n a, c, g, ort <220> <221> VARIANT <222> 181, 182 <223> Xaa any anm no aci d <220> <221> VARIANT <222> 183 <223> Xaa Phe, Ser, Tyr, or Cys <400> 311 gggatt at gt t gggggccac at ct gaaaat agt tgct ggg gaaacggcac ccacaccgct gatcttgaag gagaaccacc at ccat caca cct acgat gc at g acg t cg at g aat 11! I'bt Thr Ser I'bt Asn ct g Le u cgc Ar g gcg Al a ac g Thr gca Al a t cc Ser gcg Al a at g vb t at t I Ie t gg Tr p cgt Ar g ggt G31y cat Hi s tt g Le u ccg Pr o ggg G31y 120 tt g Le u gag O31u gct Al a gt g ValI tt g Le u cgg Ar g gt t ValI at g vb t gct Al a t ac Tyr gca Al a g at As p t at Tyr 75 gcg Al a gt g ValI at t I e ac g Thr ag c Ser gt g ValI cga Ar g gag O31u c ag O31n gt t ValI gt g ValI ct g Le u aaa Ly s tt g Le u 45 gt g ValI tt g Le u at t I Ie cct Pr o g at As p 125 t ct Ser aag Ly s c ag O31n gt t ValI ggc G31y c ag O31n gcg Al a 110 gcc Al a g ac As p 15 g aa (31u g at As p cga Ar g aag Ly s gcc Al a 95 gcg Al a cgc Ar g c ag O31n ag c Ser cct Pr o act Thr at t I Ie ggt O31y cgg Ar g g ac As p 1 gct Al a gt g ValI aat As n gag O31u gct Al a t ct Ser gct Al a aag Ly s gt g ValI ccc Pr o ggt O31y g at As p ccg Pr o tt g Le u cga Ar g c ag O31n 130 aag Ly s gcc Al a g aa O31u g aa O31u ac g Thr ggg O31y 1 00 cgg Ar g gcc Al a gct gt cgct gag at t cag tcg gat ggg cac cgc ct gaac at caat tt g Page 403 seqI i st car r ect ed. t xt Ala ValI Ala O31u IIe O31n Se r Asp 31y His Ar g L eu As n Il e Asn Leu 135 ct a Le u 1 50 g ac As p nnn Xaa ac c Thr gcg A a t ac Tyr 230 aat As n ct t Le u gag O31u ggt O31y cca Pr o 310 cgc Ar g ct g Le u ct c Le u c ag O31n gag O31u 390 g aa O31u gt g ValI t ct Ser aat As n 200 gcg A a g at As p gag O31u g at As p gt c ValI 280 aat As n gcc A a ct c Le u gt t ValI gt g ValI 360 at g vb t ct g Le u gt g ValI tt g Le u 1 70 gt g ValI gt t ValI aaa Lys5 cgc Ar g cat Hi s 250 ct c Le u ac a Thr cct Pr o g aa O31u t gg Tr p 330 99c O31y cgt Ar g t cc Ser t ac Tyr t t g Le u 155 t t g Le u gca A a gt g ValI 99c O31y ct g Le u 235 gaa O31u ggt O31y 99c O31y at g vb t cct Pr o 315 ac g Thr cac Hi s 999 O31y g at As p gt a ValI 395 140 99c O31y cgc Ar g t cg Ser g aa O31u ac g Thr 220 act Thr ct a Le u gca A a 999 O31y gag O31u 300 t cc Ser at g vb t aac As n gt a ValI c ag O31n 380 cca Pr o cga Ar g cgt Ar g c ag O31n c ag O31n 205 aag Lys5 at g vb t g aa O31u at c I Ie gcg A a 285 c ac Hi s aaa Lys5 cgc Ar g ct t Le u gcg A a 365 gcg A a gcc A a aag Lys5 ccg Pr a at t I Ie 1 90 ct g Le u tt c Ph e gag O31u gcc A a c ag O31n 270 ggc O31y gt c ValI c aa O31n aaa Lys5 tt c Ph e 350 g ac As p cgc Ar g gt g ValI g aa O31u g at As p 1 75 t cg Ser ac a Thr at c I Ie gt g ValI gga O31y 255 g ac As p gt t ValI c ac Hi s gcc A a g aa O31u 335 g ac As p cgt Ar g gcc A a cgc Ar g gca A a 1 60 gt g ValI at g vb t cct Pr a aac As n tt c Ph e 240 at t I Ie tt g Le u aag Ly s gcg A a ac c Thr 320 aac As n at a I Ie gt g ValI gt c ValI cca Pr a 400 145 gcg A a g aa O31u t gg Tr p tt a Le u ct g Le u 225 aag Ly s gt g ValI gcg A a gt t ValI c ag O31n 305 g at As p at g vb t gca A a gag O31u ag c Ser 385 c aa O31n aag Ly s t at Tyr ggt O31y t at Tyr 210 g ac As p cgg Ar g tt g Le u c ag O31n cgc Ar g 290 at c I Ie gcc A a gag O31u tt c Ph e tt c Ph e 370 gt t ValI g aa O31u cat Hi s gt g ValI tt c Ph e 1 at a I Ie at g vb t ct g Le u c ag O31n tt c Ph e 275 ct g Le u ac c Thr aat As n ggc O31y gca A a 355 g aa O31u g ac As p tt c Ph e tt g Le u t cn Ser 1 g aa O31u gag O31u gag O31u ct c Le u gcg A a 260 ggc O31y gt c ValI ggc O31y t ac Tyr ct g Le u 340 cat Hi s at g vb t gt c ValI g ac As p g at As p 1 nnn Xaa g ac As p ccc Pr a g aa O31u t cc Ser 245 t ac Tyr cgc Ar g aag Ly s t gg Tr p aag Ly s 325 cgc Ar g tt g Le u ct g Le u ggt O31y gt g ValI 405 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 gcc att tct tac ctc gt g cgc cgc ctc gag gaa aac gcc gcg agc gaa Page 404 Ala lie Ser Tyr Leu 410 seql i st cor r ect ed. t xt Val Arg Arg Leu u u Asn Al a Al a Ser G u 420 aac Asn aag Lys ct c Leu aaa Lys 470 gac Asp gt c Val gag G u gac Asp at t SIle 550 at c SIle ccg Pr o gcc Al a gt c Val gga Gy 630 ccc Pr o tgg Tr p at g Met gag G u 440 gac Asp acg Thr aac Asn acc Thr ttg Leu 520 gct Al a t ac Tyr gt g Val at c SIle gaa G u 600 gt g Val act Thr aag Lys gcc Al a gcc Al a agc Ser ccc Pr o ct c Leu gcg Al a 490 gca Al a gag G u gcg Al a acc Thr gcc Al a 570 gaa G u gac Asp acc Thr gca Al a agc Ser 650 gtt Val at c l Ie cgc Ar g gcg Al a gac Asp 475 ct c Leu gag G u ggg G y tgg Tr p gcc Al a 555 gcg Al a gcc Al a gac Asp ccg Pr o gca Al a 635 caa G n ccc Pr o ttc Phe ttc Phe ccc Pr o 460 gcc Al a at c SIle ccc Pr o gac Asp gca Al a 540 gag G u gag G u at t l Ie gt a Val ccc Pr o 620 ct c Leu cat Hi s cgc Ar g gac Asp cgc Ar g 445 ggc G y ccc Pr o caa G n ggt G y gt c Val 525 gcg Al a at t SIle gt g Val gat Asp gac Asp 605 tgg Tr p gcg Al a tgc Cys gag G u ct c Leu 430 gcc Al a ccc Pr o ct c Leu aac Asn tgg Tr p 510 gac Asp cgc Ar g ttg Leu G y ttc Phe 590 aat Asn aat Asn gcg Al a t cc Ser gtt Val 670 415 gac Asp t cc Ser aac Asn gt c Val caa G n 495 ttg Leu aag Lys cca Pr o cgc Ar g aaa Lys 575 gcc Al a gcg Al a ttc Phe ggc G y gct Al a 655 ct g Leu gcc Al a at a SIle cac Hi s cca Pr o 480 cag G n gaa G u ct a Leu gcc Al a gt g Val 560 gct Al a cgc Ar g gaa G u ccc Pr o gct Al a 640 gca Al a cat Hi s gac Asp tct Ser aca Thr 465 ttt Phe tgg Tr p aaa Lys at t SIle cgt Ar g 545 cga Ar g gt g Val t ac Tyr ttc Phe at c SIle 625 ggc G y gt g Val tgc Cys aac Asn gac Asp 450 caa G n at c SIle gcc Al a caa G n aac Asn 530 gaa G u cgc Ar g gaa G u t ac Tyr acc Thr 610 gcg Al a gt g Val gt c Val at t SIle ccg Pr o 435 ct c Leu gac Asp aac Asn aca Thr aca Thr 515 gat Asp cgc Ar g gga G y caa G n gcg Al a 595 cca Pr o at c l Ie at c SIle gaa G u t ac Tyr 675 t cc Ser gcc Al a cgc Ar g gag G u aaa Lys 500 aag Lys gt g Val gct Al a cac Hi s acc Thr 580 cat Hi s gat Asp ccc Pr o cac Hi s gcc Al a 660 cca Pr o ttc Phe acg Thr agc Ser ccc Pr o 485 gcc Al a ccg Pr o cgc Ar g gag G u ct g Leu 565 gac Asp ttg Leu cgc Ar g gct Al a aaa Lys 645 ct c Leu gct Al a 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 aat cgc gat gtt gga tgt gcg ttg atc agc cat gaa cac gtc gac cgc Page 405 seql i st cor r ect ed. t xt Asn Arg Asp Val Gy Cys Ala Leu IIe Ser His G u His Val Asp Arg 680 685 690 gtc att ttg acc ggc tcc tcc gag acc gcc gcg atg ttc tcc tcc tgg 2227 Val lie Leu Thr G y Ser Ser G u Thr Ala Ala Met Phe Ser Ser Trp 695 700 705 cga cca gaa ctc acc atc aac ggc gaa acc tcc ggc aaa aac gcc atc 2275 Arg Pro u Leu Thr Ile Asn Gy u Thr Ser y Lys Asn Ala lie 710 715 720 725 gtg gtc acc cca tct gcc gac cgc gac ctc gcc gtc gcc gac ctg gtg 2323 Val Val Thr Pro Ser Al a Asp Arg Asp Leu Ala Val Ala Asp Leu Val 730 735 740 aaa tcc gcc ttc ggc cat gca gga caa aaa tgt tcc gca gcc tcc ctc 2371 Lys Ser Ala Phe G y His Ala Gy G n Lys Cys Ser Ala Ala Ser Leu 745 750 755 ggc atc ttg gta ggc agc gtc tac gaa tca gaa cgc ttc cgg aaa cag 2419 Gy Ile Leu Val y Ser Val Tyr u Ser G u Arg Phe Arg Lys G n 760 765 770 ctg gta gac gcc gca tcc tca ctc atc gtc gac tgg cct acc aac ccc 2467 Leu Val Asp Ala Ala Ser Ser Leu lie Val Asp Trp Pro Thr Asn Pro 775 780 785 tcc gca acc gtc gga cca ctc acc gaa ctc ccc agc gat aaa ctc cac 2515 Ser Ala Thr Val Gy Pro Leu Thr G u Leu Pro Ser Asp Lys Leu His 790 795 800 805 cac gcc cta acc acc ctc gaa gaa gga gaa agc tgg ctg ctg aaa ccc 2563 His Ala Leu Thr Thr Leu G u u G y u Ser Trp Leu Leu Lys Pro 810 815 820 cga caa ctc gac gac acc ggc cga ctc tgg tca ccc ggc atc aaa gaa 2611 Arg G n Leu Asp Asp Thr Gy Arg Leu Trp Ser Pro G y lie Lys u 825 830 835 ggc gtc aaa cca gga acc ttc ttc cac ctc aca gaa gta ttc gga cca 2659 Gy Val Lys Pro Gy Thr Phe Phe His Leu Thr G u Val Phe Gy Pro 840 845 850 gtc ctc ggc ctg atg aaa gcc acc gac ctc aat gaa gcc atc gaa ttc 2707 Val Leu G y Leu IVet Lys Ala Thr Asp Leu Asn u Al a lIe G u Phe 855 860 865 caa aac ggc aac gac ttc gga ctc acc ggc gga ctc caa tcc ctc gac 2755 G n Asn y Asn Asp Phe y Leu Thr Gy y Leu G n Ser Leu Asp 870 875 880 885 gcc gac gaa gtc cgc acc tgg ctt gac cac gtc gat gtc gga aac gcc 2803 Ala Asp G u Val Arg Thr Trp Leu Asp His Val Asp Val G y Asn Ala 890 895 900 tac gtc aac cgc ggc atc acc ggc gcc att gtc caa cgc caa tcc ttc 2851 Tyr Val Asn Arg Gy Ile Thr y Ala Ile Val G n Arg G n Ser Phe 905 910 915 gga ggc tgg aaa aaa tcc tcc gtc ggc ctc gga tcc aaa gcc gga gga 2899 Sy y Trp Lys Lys Ser Ser Val y Leu y Ser Lys Ala y y 920 925 930 ccc aac tat gtc atg ctc atg gga acc tgg gcc gac gcg cca agc cac 2947 Pro Asn Tyr Val IVet Leu IVet G y Thr Trp Ala Asp Ala Pro Ser His 935 940 945 cac gcc cca cgc gaa aca aac ccg ctg atc agc aaa ctg gat ctc ccc 2995 Page 406 seql i st cor r ect ed. t xt His Ala Pro Arg u Thr Asn Pro Leu Ile Ser Lys Leu Asp Leu Pro 950 955 960 965 ggaagaa gag ctc gaa tgg ctc gaa aaa gcc aac gcc agc gat gaa aca G y G u G u Leu G u Trp Leu G u Lys Ala Asn Ala Ser Asp G u Thr 970 975 980 gca tgg aac acg gaa ttc ggc agc cca cgc gac ccc tcc ggc ctc gat Ala Trp Asn Thr G u Phe y Ser Pro Arg Asp Pro Ser y Leu Asp 985 990 995 gta gaa gcc aac att ttc cgc tac cga cca gca Val G u Ala Asn Ile Phe Arg Tyr Arg Pro Ala 1000 1005 3043 3091 3124 <210> <211> <212> <213> <220> <221> <222> <223> <220> <221> 312 1008 PRT Corynebacter i um gl ut ai cum VARI ANT 181, 182 Xaa any am no acid VARI ANT <222> 183 <223> Xaa Phe, Ser, Tyr, or Cys <400> 312 bet Thr Ser 1 Al a Val Asp Val Pro Asn Asn G y Leu G u Asp Ar g Ala Pro Ser Ser Leu Val Al a Ar g II e 115 Lys G n Phe 130 Leu Asn Ile 145 Al a Lys Hi s G u Tyr Val IVet Asn Leu Pro lie u 5 Val Asp Phe Al a bet Ar g G n Lys Leu Asp 165 Xaa Ar g Al a Thr Al a 70 Ser Al a Met Al a Leu 150 Asp Xaa Ser Lys Val Al a Phe Leu Val Val 135 Sy Thr Xaa Leu Leu Phe Al a Asp G n 105 Hi s G u Al a Ar g Ser 185 Leu 10 G u Al a Val Leu Ar g 90 Val Met SIle Val Leu 170 Al a Tyr Al a Asp Tyr Al a Val SIle G n Leu 155 Leu Leu Lys Leu Val Leu l Ie Pr o Asp 125 Asp Ar g Ar g Ser Lys G n Val Sy G n Al a 110 Al a Sy Lys Pr o Asp G u Asp Ar g Lys Al a Al a Ar g Hi s G u Asp 175 Val Ala Ser n lie Ser bet 190 Page 407 S Trp Oy Phe Ou Asp Thr Val Asn 195 200 eqI i st car r ect ed. t xt Tyr Val Val Ou On 205 Leu Thr Pr o Le u Le u 225 Ly s ValI A a ValI O31n 305 As p vb t A a O31u Ser 385 O31n As n As p Ser Thr 465 Ph e Tr p Ly s I Ie Ar g 545 I Ie vb t Le u O31n Ph e 275 Le u Thr As n O31y A a 355 O31u As p Ph e A a Pr o 435 Le u As p As n Thr Thr 515 As p Ar g Pr o O31u Ser 245 Tyr Ar g Ly s Tr p Ly s 325 Ar g Le u Le u O31y ValI 405 O31u Ph e Thr Ser Pr o 485 A a Pr o Ar g (31u A a Tyr 230 As n Le u O31u O31y Pr a 310 Ar g Le u Le u O31n O31u 390 A a As n Ly s Le u Ly s 470 As p ValI O31u As p I Ie 550 Ar g 215 Ar g Pr a Pr a Ar g A a 295 ValI ValI O31y Ser O31y 375 Le u I Ie Ph e Ar g I Ie 455 O31u Thr A a ValI A a 535 Le u A a As p O31u As p ValI 280 As n A a Le u ValI ValI 360 vb t Le u Ser vb t O31u 440 As p Thr As n Thr Le u 520 A a Tyr Pr a Le u Le u A a 265 As n Le u Thr Tyr A a 345 O31u A a Le u Tyr Ser 425 O31u ValI Le u Pr a A a 505 O31u O31u Ly s Ly s Ar g Hi s 250 Le u Thr Pr a O31u Tr p 330 O31y Ar g Ser Tyr Le u 410 A a Ser Pr a Le u A a 490 A a O31u A a O31y Le u 235 O31u O31y O31y Pr a 315 Thr Hi s O31y As p ValI 395 ValI I Ie Ar g A a As p 475 Le u O31u O31y Tr p Thr 220 Thr Le u A a O31y O31u 300 Ser vb t As n ValI O31n 380 Pr a Ar g Ph e Ph e Pr a 460 A a I Ie Pr a As p A a 540 Ly s vb t O31u I Ie A a 285 Hi s Ly s Ar g Le u A a 365 A a A a Ar g As p Ar g 445 O31y Pr a O31n O31y ValI 525 A a I Ie ValI O31y 255 As p ValI Hi s A a O31u 335 As p Ar g A a Ar g O31u 415 As p Ser As n ValI O31n 495 Le u Ly s Pr a As n Ph e 240 li1e Le u Ly s A a Thr 320 As n I Ie ValI ValI Pr a 400 O31u A a I Ie Hi s Pr a 480 O31n O31u Le u A a ValI 560 Thr Ala c3u Ilie Leu Arg 555 Page 408 seqi i st car r ect ed. t xt Ar g Ar g G31y Hi s Leu I e Ser Val 565 Thr Ala Ala Ou Val Gy Lys A a 575 ValI Tyr Ph e I Ie 625 G31y ValI CYS (31u vb t 705 G31y ValI Ser Ar g Tr p 785 Ser Tr p Pr o O31u O31u 865 Le u As p O31n A a 595 Pr o I Ie I Ie O31u Tyr 675 ValI Ser As n As p A a 755 Ar g Thr Ly s Le u I Ie 835 Ph e I Ie Ser G31y As p Le u Ar g A a Ly s 645 Le u A a Ar g Tr p I Ie 725 ValI Le u O31n Pr o Hi s 805 Pr o O31u Pr a Ph e As p 885 A a Pr a A a ValI O31y 630 Pr a Tr p As n ValI Ar g 71 0 ValI Ly s O31y Le u Ser 790 Hi s Ar g G31y ValI O31n 870 A a Tyr O31u Le u ValI 615 Ser Ser O31u Ar g I Ie 695 Pr a ValI Ser I Ie ValI 775 A a A a O31n ValI Le u 855 As n As p ValI I Ie O31u 600 ValI Thr Ly s A a As p 680 Le u O31u Thr A a Le u 760 As p Thr Le u Le u Ly s 840 O31y O31y O31u As n Ser 585 Le u ValI Ph e Pr a O31y 665 ValI Thr Le u Pr a Ph e 745 ValI A a ValI Thr As p 825 Pr a Le u As n ValI Ar g 905 570 O31u As p Thr A a Ser 650 ValI O31y (31y Thr Ser 730 O31y O31y A a O31y Thr 810 As p O31y vb t As p Ar g 890 O31y A a As p Pr a A a 635 O31n Pr a cys Ser I Ie 715 A a Hi s Ser Ser Pr a 795 Le u Thr Thr Ly s Ph e 875 Thr I Ie As p As p 605 Tr p A a cys O31u Le u 685 (31u O31y Ar g O31y Tyr 765 Le u Thr O31u Ar g Ph e 845 Thr Le u Le u O31y Ph e 590 As n As n A a Ser ValI 670 li1e Thr O31u As p O31n 750 O31u I Ie O31u O31y Le u 830 Hi s As p Thr As p A a 910 A a A a Ph e O31y A a 655 Le u Ser A a Thr Le u 735 Ly s Ser ValI Le u O31u 815 Tr p Le u Le u O31y Hi s 895 li1e Ar g O31u Pr a A a 640 A a Hi s Hi s A a Ser 720 A a cys O31u As p Pr a 800 Ser Ser Th r As n O31y 880 ValI ValI O31n Ar g O31n Ser Phe O31y O31y Tr p Lys Lys Ser Ser Val O31y Leu O31y Page 409 Ser Lys 930 Asp Al a 945 Lys Leu Al a Ser Pro Ser 915 Ala G y G y Pro Asn 935 Pro Ser His His Ala 950 Asp Leu Pro Gy u 965 Asp G u Thr Al a Trp 980 Sy Leu Asp Val G u 995 seql i st cor r ect ed. t xt 920 925 Tyr Val Mlt Leu lMt y Thr Trp Ala 940 Pro Arg G u Thr Asn Pro Leu I e Ser 955 960 G u Leu G u Trp Leu G u Lys Ala Asn 970 975 Asn Thr G u Phe y Ser Pro Arg Asp 985 990 Ala Asn Ile Phe Arg Tyr Arg Pro Ala 1000 1005 <210> 313 <211> 927 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(904) <223> RXC02498 <400> 313 caaggtagaa atttgtccgt gggcacttcg tgaaggtgt g aggactcgag taacatttac ccggaaagga gttggcgaaa atcctcacca ggatcgacaa atg agt gaa gag aaa 11! lVet Ser G u G u Lys ct c Leu acc Thr gt a Val ccc Pr o cct Pr o gt g Val ccg Pr o aaa Lys gt c Val gct Al a gtt Val gag Gu gtt Val tcg Ser ccg Pr o cca Pr o 120 gag G u cga Ar g gag G u aag Lys gag Gu gag Gu aac Asn agc Ser ct g Leu cga Ar g ct g Leu cac Hi s gcc Al a 75 ggc Gy gaa Gu tt g Leu at g Met cgc Ar g acc Thr t cc Ser ccc Pr o gac Asp gaa Gu acg Thr gcg Al a agg Ar g ggc G y 45 agc Ser aag Lys gt c Val acc Thr acg Thr 125 cgt Ar g cgc Ar g t cc Ser gt g Val cct Pr o gat Asp at g Met 110 ggc Gy gcc Al a 15 cgc Ar g at t SIle ccc Pr o gag Gu aag Lys 95 gt g Val gcg Al a gcg Al a agc Ser cct Pr o at c SIle ccc Pr o 80 cag G n ct g Leu ttc Phe 1 aaa Lys at c SIle gcc Al a gat Asp gcc Al a gaa Gu cgc Ar g ccc Pr o gag G u gaa Gu gtt Val gca Al a gaa Gu acc Thr at c SIle gt g Val 130 gga G y gac Asp aag Lys cca Pr o gaa Gu cct Pr o gt g Val 115 gtt Val cgc Ar g ggt G y gaa Gu gca Al a gt a Val gag Gu 100 gat Asp ccg Pr o t cc Ser ggc G y aag Lys gaa Gu gaa Gu cgt Ar g gaa Gu gca Al a gtt gcc gcc aag ccg gcg ccc gta gtg cgc gcg gag aag gac gcc gat Page 410 Val Ala Ala Lys Pro Ala Pro 135 140 seqi i st car r ect ed. t xt Val Val Ar g Al a G31u Lys 145 Asp Al a Asp gt g ValI 1 50 act Thr gag G31u gt c ValI t gg Tr p ac c Thr 230 ggt G31y ggg (31y act Thr ac c Thr g aa G31u gt t ValI 200 cgc Ar g gga G31y ag c Ser ct g Le u gt a ValI c ag (31n 1 70 aaa Ly s ct c Le u aac As n gt g ValI gt t ValI 250 gca Al a gct Al a t cc Ser gt c ValI t gg Tr p 220 at c I Ie gca Al a tt c Ph e gt g ValI tt c Ph e 1 90 gt a ValI gt c ValI c ac Hi s at c I Ie gca Al a g aa G31u 1 75 gcg Al a tt c Ph e gct Al a gct Al a gt g ValI 255 gag G31u 1 g aa G31u at c I Ie ct c Le u gt t ValI tt g Le u 240 ggc (31y g aa G31u g ac As p at g vb t tt t Ph e 210 gca Al a ac c Thr gt c ValI gt c ValI gag G31u at g vb t 1 g aa G31u gt c ValI t ca Ser at g vb t g at As p gag G31u 1 gcg Al a at g G31y cgt Ar g ac g Thr 260 at c gt c at g I Ie Va Ilb ~t t aat t tgt cg tt t tgggccc ccg <210O> 314 <21 1> 268 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 314 l'bt Ser G31u G31u Lys Leu Thr Val Al a 1 5 Lys Gu Gy Arg Ser Thr Asp Ala Pro li1e G31u As p 31y G31y Val Ser Val Al a 40 Al a Val Lys G31u Lys Pr o Al a G31u Ser As p Al a Pr o Al a G31u Pr o G31u ValI Val 70 Al a 31u G31u Val G31u Val Al a Se r Val G31u Thr Pr o G31u Ar g Pr o Al a Pr o Ser 100 105 Ar g I Ie Val As p G31u Lys As p Pr o I Ie 115 120 Pr o Val Val Pr o Al a Val Al a Al a Lys 130 135 03 u Leu I 10 Ar g Ar g 03 u Leu Lys Hi s 03 u Al a 1 (31u 1y~ 90 As n (1u Ser Leu- Pr o Al a I Page 411 Al a Ar g G31y Ser Ly s ValI Thr Thr 125 ValI Al a Ar g I Ie Pr o (31u Ly s ValI Al a Ar g O31u 145 ValI ValI I Ie G31y Le u 225 Ar g Le u As p ValI (31u vb t 1 95 (31u ValI Ser vb t As p As n 1 65 Pr o I Ie Le u ValI As p 245 Ph e (31u As p O31n G31y O31u 215 Le u Ph e Pr o seqi i st car r ect ed. t xt Thr Ala Val Lys Ala Asp 1 Thr Thr 03 n Nib t A a Val 1 70 O3 u As n Lys lVb t Ser Val 1 Val Val Leu 03 y Val Val 200 205 Ar g Leu As n Lys Tr p I Ie 220 G31y l'b t Val G31y I Ie I Ie 235 Ser l'b t Val Leu Ala (31y 250 Leu Ala I Ie Val l'b t 265 A a O31u 1 A a Ph e A a A a ValI 255 <210O> 315 <21 1> 774 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (751) <223> RXA01491 <400> 315 aacggt gt ga aacacat t ga agt gt cgt t g ggt t at ccgc gggcagcat g t gt ggccat a t ccagt gat g gaggt ggaca gcgaat ggga gcat tggt ct tt g Le u gt t ValI gt a ValI t gg Tr p ccc Pr o t ct Ser cca Pr o tt a Le u c ac Hi s gca A a tt g Le u t ca Ser t cg Ser cat Hi s gt t ValI c ag O31n ggg G31y ac c Th r gca A a t t c Ph e g at As p gca A a gaa O31u 75 cac Hi s aag Ly s cat Hi s at t I Ie ct c Le u cga Ar g act Th r tt t Ph e c ag O31n ag a Ar g 45 c aa O31n gga O31y g ac As p t ct Ser tt g Le u aaa Ly s gct A a at g vb t gga (31y tt c Ph e 15 cat Hi s gca A a tt g Le u cca Pr a tt c Ph e 95 at t I Ie ccg Pr a gag O31u gga O31y tt g Le u cga Ar g at g vb t 1 aaa Ly s tt g Le u tt t Ph e cga Ar g t gg Tr p gct A a ct g gat gag t ct Leu Asp 03 u Ser act Th r g aa O31u gga O31y g at As p cct Pr a gct A a g at As p gca A a gcc A a ggt O31y t cg Se r gt g ValI 1 00 cca cga ttg ttg gtg cgt tct atg gga ttg gat gcc gaa cct gcg gag Page 412 seql i st cor r ect ed. t xt Pro Arg Leu Leu Val Arg Ser IVet Gy Leu Asp Ala G u 105 110 ccg ttg ccc aag gat gtt ttg ggt tca atc gct cgg gtg Pro Leu Pro Lys Asp Val Leu y Ser lie Ala Arg Val 120 125 130 cct caa ctt aag cgc ttg gag gaa caa ggt gtg cac tgc Pro G n Leu Lys Arg Leu G u G u G n y Val His Cys 135 140 145 ctg ctg ttt tgt gcc aag gaa gca aca tac aaa gcg tgg Leu Leu Phe Cys Ala Lys G u Ala Thr Tyr Lys Ala Trp 150 155 160 acg cat agg tgg ctt ggt ttt gaa caa gct gag atc gac Thr His Arg Trp Leu y Phe Gu Gn Ala Gu Ile Asp 170 175 gat ggc act ttt gtg tcc tat ttg ctg gtt cga cca act Asp y Thr Phe Val Ser Tyr Leu Leu Val Arg Pro Thr 185 190 ttt att tca ggt aaa tgg gta ctg cgt gat ggt tat gtc Phe Ile Ser y Lys Trp Val Leu Arg Asp y Tyr Val 200 205 210 act gca gtg act tgaactggat ggagaggata cct Thr Al a Val Thr 215 <210> 316 <211> 217 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 316 lIet Leu Asp u Ser Leu Phe Pro Asn Ser Ala Lys Phe 1 5 10 Lys Thr G y Asp Ala Val Asn Leu Asp His Phe His G n 25 Leu G u Lys Ala Leu Val Ala His Ser Val Asp lie Arg 40 Phe G y Asp Al a Arg Trp Cys Al a His G n Al a Leu G n 55 Arg Asp Ser G y Asp Pro lie Leu Arg G y G u Arg G y 70 75 Trp Pro Ser Ser Val Ser G y Ser Leu Thr His Thr Asp 90 Ala Ala Val Val Ala Pro Arg Leu Leu Val Arg Ser IVet 100 105 Ala G u Pro Ala G u Pro Leu Pro Lys Asp Val Leu y 115 120 125 Arg Val y u Ile Pro n Leu Lys Arg Leu u u 130 135 140 His Cys Ala Asp Arg Leu Leu Phe Cys Ala Lys G u Ala 145 150 155 Page 413 Pr o 115 ggg G y gcg Al a ttc Phe ttg Leu cca Pr o 195 at a SIle Al a gag G u gat Asp ccg Pr o cgt Ar g 180 gt g Val gct Al a G u att SIe cgc Ar g ctg Leu 165 gat Asp ccg Pr o gcg Al a Ser Phe Il e Leu His Pro Lys Al a G u Al a Leu G y IVet Pro Leu G y Phe Arg G y Leu Asp 110 Ser Ile Ala Sn G y Val Thr Tyr Lys seqi i st car r ec Ala Trp Phe Pro Leu Thr His Arg Trp Leu Gy 165 170 li1e Asp Leu Ar g As p As p G31y Thr Phe Val Ser 180 185 Pr o Thr Pr o Val Pr o Phe I Ie Ser G31y Lys Tr p 195 200 Tyr Val I Ie Ala Ala Th r Ala Val Thr 210 215 <210O> 317 <21 1> 1287 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CDS <222> (1264) <223> RXA02155 <400> 317 t cggcgt cgc acct taaagt agcgcct taa agcggcgct t gcaaacacaa caaacacatc taattcagta ggagttccac ted. t xt Ph e G3 u G3 n A a G3 u 1 Tyr Leu Leu Val Ar g 1 Val Leu Ar g Asp G31y 205 caaaccaagc gccctaacca atg gca gaa aaa ggc 11! l'bt A a G3 u Lys G3 y at t I Ie aaa Ly s gag a31u cct Pr o gt t ValI gag a31u tt g Le u tt g Le u gag a31u ac c Thr gcg A a t ct Ser t cc Ser aag Ly s t ac Tyr g at As p g at As p ccg Pr o 120 gct A a g ac As p aaa Ly s aat As n gcg A a ag c Ser gct A a cgt Ar g g at As p g at As p ggt a31y gt g ValI ggc a31y cct Pr o gcc A a cga Ar g ggt a31y 75 gag a31u at t I Ie aag Ly s gac As p g at As p tt c Ph e g ac As p gt g ValI gag a31u aat As n t ct Ser ggt a31y ct c Le u aat As n 140 aag Ly s gt t ValI at g vb t tt t Ph e 45 aac As n gct A a gt t ValI gt g ValI aat As n 125 ggt a31y g aa a31u gct A a gcg A a ac a Thr gt t ValI aat As n t ct Ser t gt cys 110 gca A a gca A a ac c Thr t ct Ser 15 tt g Le u cgt Ar g gct A a gcg A a cat Hi s 95 t cc Ser ggt a31y gct A a gca A a gt g ValI aac As n g at As p t gt cys ct a Le u act Thr at t I Ie gct A a 1 ac g Thr gt t ValI cga Ar g ggc a31y aat As n gct A a ggt a31y g at As p gcc A a 145 ac c Thr aac As n gt t ValI c ag a31n ggt a31y c aa a31n ct t Le u c ag a31n 130 aag Ly s gcg A a c ag a31n tt c Ph e at c I Ie ct g Le u aat As n at t I Ie 115 ct g Le u gcg A a ggt a31y ggt a31y gca A a ag g Ar g c ag a31n tt g Le u 1 00 ggt a31y ac c Thr at c I Ie gt c gt g Val Val ttt gct gat ggt tgg Ph e Ala Asp a31y Tr p Page 414 seql i st cor r ect ed. t xt 150 155 160 165 act gtc ggc gga atg ggc aag ggc gtg ggc atg atg gcg ccg tct ctt 643 Thr Val G y G y M t G y Lys G y Val G y MN t M t Ala Pro Ser Leu 170 175 180 gcc acc atg ctg gtc tgc ttg acc act gat gca tcc gtt act cag gaa 691 Ala Thr Mbt Leu Val Cys Leu Thr Thr Asp Ala Ser Val Thr n G u 185 190 195 atg gct cag atc gcg ctg gct aat gct acg gcc gtt acg ttt gac acc 739 IV t Ala G n I e Ala Leu Ala Asn Ala Thr Ala Val Thr Phe Asp Thr 200 205 210 ctg gat att gat gga tca acc tcc acc aat gac acc gtg ttc ctg ctg 787 Leu Asp I e Asp G y Ser Thr Ser Thr Asn Asp Thr Val Phe Leu Leu 215 220 225 gca tct ggc gct agc gga atc acc cca act cag gat gaa ctc aac gat 835 Ala Ser y Ala Ser Gy lie Thr Pro Thr n Asp u Leu Asn Asp 230 235 240 245 gcg gtg tac gca gct tgt tct gat atc gca gcg aag ctt cag gct gat 883 Ala Val Tyr Ala Ala Cys Ser Asp lie Ala Ala Lys Leu G n Ala Asp 250 255 260 gca gag ggt gtg acc aag cgc gtt gct gtg aca gtg gtg gga acc acc 931 Ala G u y Val Thr Lys Arg Val Ala Val Thr Val Val y Thr Thr 265 270 275 aac aac gag cag gcg att aat gcg gct cgc act gtt gct cgt gac aat 979 Asn Asn G u G n Ala lle Asn Ala Ala Arg Thr Val Ala Arg Asp Asn 280 285 290 ttg ttc aag tgc gca atg ttt gga tct gat cca aac tgg ggt cgc gtg 1027 Leu Phe Lys Cys Ala IVet Phe y Ser Asp Pro Asn Trp y Arg Val 295 300 305 ttg gct gca gtc ggc atg gct gat gct gat atg gaa cca gag aag att 1075 Leu Ala Ala Val Gy IVet Ala Asp Ala Asp IVet u Pro u Lys Ile 310 315 320 325 tct gtg ttc ttc aat ggt caa gca gta tgc ctt gat tcc act ggc gct 1123 Ser Val Phe Phe Asn G y G n Ala Val Cys Leu Asp Ser Thr y Ala 330 335 340 cct ggt gct cgt gag gtg gat ctt tcc ggc gct gac att gat gtc cga 1171 Pro y Ala Arg G u Val Asp Leu Ser y Ala Asp Ile Asp Val Arg 345 350 355 att gat ttg ggc acc agt ggg gaa ggc cag gca aca gtt cga acc act 1219 Ile Asp Leu y Thr Ser Gy G u Gy G n Ala Thr Val Arg Thr Thr 360 365 370 gac ctg agc ttc tcc tac gtg gag atc aac tcc gcg tac agc tct 1264 Asp Leu Ser Phe Ser Tyr Val G u Ile Asn Ser Ala Tyr Ser Ser 375 380 385 taaaaagaaa cagcactcca act 1287 <210> 318 <211> 388 <212> PRT <213> Cor ynebact eri um gl ut arr cum <400> 318 IV t Al a u Lys Gy IIe Thr Ala Pro Lys y Phe Val Ala Ser Al a Page 415 seqI i st car r ect ed. t xt Il e Lys Al aSer Thr ValI Ar g G31y As n A a G31y As p A a 145 Ph e vb t Ser ValI Thr 225 As p Ly s ValI ValI As n 305 (31u As p As p A a (31n Ph e I Ie Le u As n I Ie 115 Le u A a As p Pr o Thr 1 95 Ph e Ph e Le u O31n G31y 275 Ar g G31y O31u Thr As p 355 Pr o A a A a G31y G31y O31u A a Nibt Tr p 1 65 Le u O31u Thr Le u As p 245 As p Thr As n ValI I Ie 325 A a Ar g (31u Pr o ValI 70 O31u Le u Le u O31u Thr 150 Thr A a vb t Le u A a 230 A a A a As n Le u Le u 310 Ser Pr o I Ie Ph e ValI Le u Ly s O31u Le u G31y 135 Thr ValI Thr A a As p 215 Ser ValI O31u As n Ph e 295 A a ValI G31y As p Ser 40 Ly s Tyr As p As p Pr o 120 A a As p G31y vb t O31n 200 li1e G31y Tyr G31y O31u 280 Ly s A a Ph e A a Le u 360 G31y A a ValI As n A a Ser 1 05 vb t Le u Thr G31y Le u 1 85 li1e As p A a A a ValI 265 O31n cys ValI Ph e Ar g 345 G31y Asn Pro Asp I'bt A a Ser A a Ar g 90 As p As p G31y ValI vb t 1 70 ValI A a G31y Ser A a 250 Thr A a A a G31y As n 330 O31u A a Ar g G31y 75 O31u I Ie Ly s As p As p 1 55 G31y cys Le u Ser G31y 235 cys Ly s I Ie Nibt Nibt 315 G31y ValI ValI O31u As n Ser G31y Le u As n 140 Ly s Ly s Le u A a Thr 220 li1e Ser Ar g As n Ph e 300 A a O31n As p Ph e As n A a ValI ValI As n 125 O31y O31u O31y Thr As n 205 Ser Thr As p ValI A a 285 O31y As p A a Le u A a Thr ValI As n Ser cys 110 A a A a Thr ValI Thr 1 A a Thr Pr a I Ie A a 270 A a Ser A a ValI Ser 350 Le u Ar g A a A a Hi s Ser O31y A a ValI O31y 1 As p Thr As n Thr A a 255 ValI Ar g As p As p cys 335 (31y ValI As n As p cys Le u Thr I Ie A a ValI 1 vb t A a A a As p O31n 240 A a Thr Thr Pr a vb t 320 Le u A a Th r Se r 03 y 03 u 03 y 03 n A a 365 Page 416 seqi i st car r ect ed. t xt Thr Val Arg Thr Thr Asp Leu Ser Phe Ser Tyr Val 03 u I I e Asn Ser 370 375 380 A a Ty r Se r Se r 385 <210> 319 <211> 1074 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1051) <223> RXA02156 <400> 319 aaccact gac ct gagct t ct cct acgt gga gat caact cc gcgt acagct ct t aaaaaga aacagcact c caact aacaa gcagggaaaa gggcacaggc at g aat gac t tg at c l'bt Asn Asp Leu I I e aaa Ly s cca Pr a gga G31y at g vb t ggt G31y gag G31u gt g ValI at c I Ie ggc G31y act Thr 1 50 tt g Le u tt a Le u tt g Le u gcc A a tt c Ph e cct Pr a aag Ly s at g vb t t ct Ser 120 tt t Ph e at t I Ie g at As p t ct Ser c ac Hi s gt g ValI cgc Ar g at t I Ie ggt G31y ct c Le u ggc G31y gcg A a tt g Le u at c I Ie 1 70 gt g ValI cgc Ar g g at As p gt g ValI gag G31u cgt Ar g ggt G31y t ac Tyr aag Ly s 140 gga G31y gcc A a gca A a aag Ly s ct c Le u gca A a ct a Le u ac c Thr gt c ValI 110 gt g ValI at g vb t at c I Ie cgc Ar g aat As n 15 at t I Ie aag Ly s aaa Ly s aac As n act Thr 95 ggt G31y gga G31y gt c ValI at t I Ie at t I Ie 1 75 gt c ValI gt t ValI gct A a cca Pr a cgt Ar g cct Pr a cgc Ar g ac c Thr aac As n aat As n 1 cct Pr a gct A a gt g ValI tt t Ph e gt g ValI ggt G31y gt c ValI tt a Le u ggt G31y 130 g at As p g at As p gt c ValI gag G u aaa Ly s gct A a gt g ValI ct c Le u at g Nibt gt t ValI 115 gag G3u ggc G3y gcc A a t ct Ser gcg A a t at Tyr gcc A a c ac Hi s c ag (31n g ac As p 1 00 ggt G31y g at As p gt a ValI t ct Se r ac g Th r 1 Page 417 seqI i st car r ect ed. t xt gct cca ggc gaa gac ggc cag att Ala Pro G1y O3uAsp Oy On Ilie 1 85 t ac aac at t aac Tvr As n I I e As n gca ggt gct ttg gct gca gcg at t ggt gca Al a 03 y Al a Leu Al a Al a Al a Il e 03 y Al a 200 205 acc aat gt g gaa ggt ct g t ac acc gat t gg Thr Asn Val O31u G31y Leu Tyr Thr Asp Tr p 215 220 gtg tcc aag atc aag gcc acc gag ctg gag Val Ser Lys Ilie Lys Al a Thr O31u Leu O31u 230 235 gat t cc ggc at g at t cca aag at g gag t ct Asp Ser 31y 'b t Ilie Pr o Lys I'b t O31u Ser 250 255 ggg gga gt a agc gct gct cat gt c at t gac G31y G31y Val Ser Al a Al a Hi s Val Ilie Asp 265 270 gt g t tg ct g gag ct t t tg acc at g ggt gga Val Leu Leu G31u Leu Leu Thr [Vb t 31y G31y 280 285 ccg gat gt t t tt gat cgg gag aat t at cct Pr o Asp Val Phe Asp Ar g 03 u Asn Tyr Pr o 295 300 aaa gac gac aag gat ggg gaa ctg taaatga Lys Asp Asp Lys Asp 03 y 03 u Leu 310 315 <210> 320 <211> 317 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 320 Ntbt Asn Asp Leu I I e Lys Asp Leu 03 y Ser 1 5 10 Leu Ala Ou Ala Leu Pro Trp Leu On His 25 Val Val Lys Tyr 31y G31y As n Al a I'bt Val 40 Al a Phe Al a Al a Asp I'b t Val Phe Leu Ar g Val Val Val Hi s G31y G31y G31y Pro O31n Ilie 70 Val G31y Leu O31n G31y O31u Phe Lys G31y G31y 90 O31u Val I'b t Asp Ilie Val Ar g I'b t Val Leu 100 105 Asp Leu Val O31y Leu Ilie Asn Ser Hi s O31y 115 120 Ser 03 y 03 u Asp Al a 03 y Leu Phe Thr Al a Pag g aa (31u cct Pr o gcc Al a 240 t gc cys ggc O31y at t I Ie g aa a u gcc gat acc gca Al a Asp Th r Al a 1 ct g ct g gt t ct c Leu Leu Val Leu 210 aag agc t ca ct g Lys Ser Ser Leu ct t ccg gga ct t Leu Pr o O31y Leu 245 aac gcg gt g cgt Asn Al a Val Ar g 260 at c gcg cac t cg Il e Al a Hi s Ser 275 acg at g gt g ct g Thr [vb t Val Leu 290 acc gt t t tt aga Thr Val Phe Ar g 691 739 787 835 883 931 979 1 027 1 074 jca cgct ggaaac tt g 31u~ Phe As p Thr Ser Phe Phe Pr o 31n I e 418 Ar g As p As p O31y vb t ValI O31n Al a 125 Ar g Al a Lys5 Le u Al a Le u Thr ValI 110 ValI vb t As n lie As n Th r O31y O31y ValI seqI i st car r ect ed. t xt 135 Pro Thr Asp Ilie Gy Leu 140 I Ie 145 ValI ValI As n Ar g As p 225 li1e Le u Ar g G31y G31y 305 G31y A a Ser As p 1 95 Le u Ser Pr o A a A a 275 vb t ValI Ser 1 65 li1e A a Le u Le u Le u 245 Ar g Ser Le u Ar g 150 Le u A a A a Thr ValI 230 As p G31y ValI Pr o Ly s 310 Pr o G31y As n 215 Ser Ser G31y Le u As p 295 As p I Ie G31u 1 Le u Gu le vb t Ser 265 G31u Ph e Ly s ValI 1 G31u G31y A a Le u A a 235 Pr o A a Le u Ar g G31y 315 G31y A a (31n A a Tyr 220 Thr Ly s Hi s Thr G31u 300 (31u As p G31y I Ie I Ie 205 Thr G31u vb t ValI vb t 285 As n Le u le le 1 As n A a Tr p G31u Ser 255 As p G31y Pr o <210O> 321 <21 1> 903 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (880) <223> RXN02153 <400> 321 cccact tgca gtt t tagct g t aggt gggt t t t tgcat gt c cccccgcaat gaat caaaaa t t tatgcat g aat aat t tgc gt g ValI gga G31y t at Tyr gca A a ggt G31y gcc A a t ct Ser ag c Ser act Th r gga G31y g aa G31u ct c Le u at g vb t gaa G31u ct a Le u ggt (31y aag Ly s cgt Ar g gga G31y at g gt t gca Val Ala 15 ct c ct t Leu Leu gca ct c A a Leu cca cac Pr o Hi s Page 41~ t aacccgt ct t t tat gcaca at g at c at g cat aac I'b t I Ie I'b t Hi s As n 1 at c gca gga gcc agt Il e A a 03 y A a Ser t ta ggc cat cca gct Leu G31y His Pr o Ala acc gcg gca t ca acc Thr A a A a Ser Thr at t ccg cag t tg gcg Ilie Pr o (31n Leu Ala seql i st cor r ect ed. t xt gat Asp gt c Val cag G n ct g Leu gga G y ctt Leu 150 gca Al a gat Asp gca Al a t ac Tyr ct c Leu 230 ctt Leu gtt Val ttt Phe gga G y aat Asn 120 tgg Tr p ggt Sy ttg Leu t cc Ser gtt Val 200 acc Thr gaa G u ccg Pr o ati caa Ile G n ct a gga Leu G y cca gat Pro Asp 105 gct gca Al a Al a cct tat Pro Tyr gct aag Al a Lys gct ct t Al a Leu 170 gta gt g Val Val 185 gca ct a Al a Leu t cc gga Ser G y gtc agc Val Ser tta cct Leu Pro 250 gac acc aca gct gaa act Asp Thr Thr Ala G u Thr 75 80 ctt cca cac gga ttc t ct Leu Pro His Gy Phe Ser 95 gtc aca gtg att gac tgt Val Thr Val lie Asp Cys 110 gat tgg gag aag ttc tac Asp Trp G u Lys Phe Tyr 125 ggc att cca gaa atg cca Gy Ile Pro G u Iet Pro 140 cgt gta gca gtg cca gga Arg Val Ala Val Pro y 155 160 ctt cct gcg gtt caa gcg Leu Pro Ala Val G n Ala 175 tcc atc acc ggc gta tca Ser lie Thr y Val Ser 190 ctt ggc tcg gaa acc atg Leu G y Ser G u Thr IVet 205 aag cac cgc cac acc ccg Lys His Arg His Thr Pro 220 gac aag cca gtc aag gtg Asp Lys Pro Val Lys Val 235 240 cgc gaa ttc tca cca ctg Arg G u Phe Ser Pro Leu 255 ct a gcc ggt Leu Al a G y gca gaa att Al a G u Ile gca gct gac Al a Al a Asp 115 ggc tca gag G y Ser G u 130 gga cac cgc G y His Ar g 145 tgt ttc cca Cys Phe Pro gga ct t at c G y Leu Ile ggt gca ggt G y Al a G y 195 ggt tca ct c G y Ser Leu 210 gaa at t gcc G u Ile Al a 225 agc ttc acc Ser Phe Thr caa ccg cac G n Pro Hi s cat Hi s gca Al a 100 ttt Phe cac Hi s gag G u acc Thr gag G u 180 aag Lys aag Lys cag G n cca Pr o ctt Leu 260 gat Asp ctt Leu cgt Ar g cag G n gct Al a ggt G y 165 cca Pr o aaa Lys gcg Al a aac Asn gtg Val 245 tgaaagaagg cgttaccgca gaa <210> 322 <211> 260 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 322 'e t I e bet His Asn Val Tyr G y Val Thr bet Thr lie Lys Val Ala 1 5 10 Ile Al a G y Al a Ser y Tyr Al a y G y u Ile Leu Arg Leu Leu 25 Leu G y His Pro Al a Tyr Ala Ser G y G u Leu G u lie G y Al a Leu 40 Thr Ala Ala Ser Thr Al a y Ser Thr Leu G y u Leu IVet Pro His Page 420 seql i st cor r ect ed. t xt I e Leu Al a Al a Gy Gy 145 Cys Gy Gy Gy G u 225 Ser G n G n Gy l e Asp 115 G u Ar g Pr o SIle Gy 195 Leu Al a Thr Hi s Al a Asp Leu Ar g G n Al a Gy 165 Pr o Lys Al a Asn Val 245 Asp 70 Val G n Leu Sy Leu 150 Al a Asp Al a Tyr Leu 230 Leu Arg Val Ile n Asp Val Phe Leu G y Leu 90 Leu y Pro Asp Val 105 G n Asn Ala Ala Asp 120 Thr Trp Pro Tyr y 135 Arg y Ala Lys Arg 155 Thr Leu Ala Leu Leu 170 Val Ser Val Val Ser 185 Ser Val Ala Leu Leu 200 Asn Thr Ser G y Lys 215 Sy G u Val Ser Asp 235 Ala Pro Leu Pro Arg 250 Thr Thr Ala G u Hi s Val G u 125 Pr o Al a Al a Thr Ser 205 Ar g Pr o Phe Phe Asp Phe bet Pr o G n 175 Val Thr Thr Lys Pr o 255 <210> 323 <211> 903 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(880) <223> FRXA02153 <400> 323 cccacttgca gttttagctg taggtgggtt tttgcatgtc taacccgtct tttatgcaca cccccgcaat gaatcaaaaa tttatgcatg aataatttgc atg atc atg cat aac IVet Ile IVet His Asn 1 gtg tat ggt gta act atg aca atc aag gtt gca atc gca gga gcc agt Val Tyr G y Val Thr IVet Thr Ile Lys Val Al a I e Al a y Al a Ser 15 gga tat gcc ggc gga gaa atc ctt cgt ctc ctt tta ggc cat cca gct G y Tyr Ala y y u lie Leu Arg Leu Leu Leu G y His Pro Ala 30 tat gca tct ggt gaa cta gaa atc gga gca ctc acc gcg gca tca acc Page 421 seql i st cor r ect ed. t xt Tyr Ala Ser y u Leu Gu Ile y Ala Leu Thr Al 45 a Ala Ser Thr gca Al a gat Asp gt c Val cag G n ct g Leu gga G y ctt Leu 150 gca Al a gat Asp gca Al a t ac Tyr ct c Leu 230 ctt Leu agc acg Ser Thr gtt att Val lile ttt cta Phe Leu gga cca G y Pro 105 aat gct Asn Ala 120 tgg cct Trp Pro ggt gct G y Al a ttg gct Leu Al a t cc gt a Ser Val 185 gtt gca Val Ala 200 acc t cc Thr Ser gaa gt c G u Val ccg tta Pro Leu ct c Leu caa G n gga G y gat Asp gca Al a t at Tyr aag Lys ctt Leu 170 gt g Val ct a Leu gga G y agc Ser cct Pr o 250 ggt gaa G y G u gac acc Asp Thr 75 ctt cca Leu Pro gtc aca Val Thr gat tgg Asp Trp ggc att G y lie 140 cgt gta Arg Val 155 ctt cct Leu Pro tcc atc Ser Ile ctt ggc Leu G y aag cac Lys Hi s 220 gac aag Asp Lys 235 cgc gaa Arg u ttg Leu aca Thr cac Hi s gt g Val gag G u 125 cca Pr o gca Al a gcg Al a acc Thr tcg Ser 205 cgc Ar g cca Pr o ttc Phe at g Met gct Al a gga G y at t SIle 110 aag Lys gaa G u gt g Val gtt Val ggc G y 190 gaa G u cac Hi s gt c Val t ca Ser cca Pr o gaa G u ttc Phe 95 gac Asp ttc Phe at g Met cca Pr o caa G n 175 gt a Val acc Thr acc Thr aag Lys cca Pr o 255 cac Hi s act Thr 80 tct Ser tgt Cys t ac Tyr cca Pr o gga G y 160 gcg Al a t ca Ser at g Met ccg Pr o gt g Val 240 ct g Leu at t I e ct a Leu gca Al a gca Al a ggc G y gga G y 145 tgt Cys gga G y ggt G y ggt G y gaa G u 225 agc Ser caa G n ccg Pr o gcc Al a gaa G u gct Al a t ca Ser 130 cac Hi s ttc Phe ctt Leu gca Al a t ca Ser 210 at t SIle ttc Phe ccg Pr o ttg Leu cat Hi s gca Al a 100 ttt Phe cac Hi s gag G u acc Thr gag G u 180 aag Lys aag Lys cag G n cca Pr o ctt Leu 260 gcg Al a gat Asp ctt Leu cgt Ar g cag G n gct Al a ggt G y 165 cca Pr o aaa Lys gcg Al a aac Asn gtg Val 245 tgaaagaagg cgttaccgca gaa <210> 324 <211> 260 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 324 Iet I I e IVet His Asn Val Tyr G y Val Thr IVet Thr lie Lys Val Ala 1 5 10 Ile Al a y Al a Ser y Tyr Al a y y u Ile Leu Arg Leu Leu Page 422 seqi i st car r ect ed. t xt Pro Ala Tyr Ala 25 G3 y G3 u L eu G3 u G3 y Al a Leu Le u Thr lIe Le u Al a Al a a31y a31y 145 CYS a31y a31y a31y a31u 225 Ser a31n Hi s Al a a31n a31y I Ie As p 115 a31u Ar g Pr o I Ie a31y 1 95 Le u Al a Thr Hi s Thr Al a As p Le u Ar g a31n Al a a31y 1 65 Pr o Ly s Al a As n ValI 245 Al a As p 70 ValI a31n Le u a31y Le u 150 Al a As p Al a Tyr Le u 230 Le u G31y Ar g ValI Le u a31n Thr 135 Ar g Thr ValI Ser As n 215 a31y Al a Thr I Ie Le u Pr o 1 05 Al a Pr o Al a Al a ValI 1 85 Al a Ser ValI Le u G31y As p Le u ValI As p a31y Ar g 1 Le u Ser Le u Ly s As p 235 Ar g lIe Le u Thr Hi s ValI a31u 125 Pr a Al a Al a Thr Ser 205 Ar g Pr a Ph e Al a a31y 110 Ly s a31u ValI ValI a31y 1 a31u Hi s ValI Ser Pr o a31u Ph e As p Ph e vb t Pr a a31n 1 ValI Thr Thr Ly s Pr a 255 <210O> 325 <21 1> 414 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 ODS <222> (391) <223> RXA02154 <400> 325 cccagaacct cggcgaagt c agcgacaagc cagt caaggt gagct tcacc ccagt gct tg caccgt tacc t cgcgaat tc t caccact gc aaccgcacct t tg aaa gaa ggc gt t 115 Leu Lys a3 u a3 y Val 1 acc gca gaa cag gct cgc gca gta tat gaa gag ttc tat gca cag gaa 163 Thr Ala Ou On Ala Arg Ala Val Tyr Ou Ou Phe Ty r Al a 31n (31u 15 Page 423 acc ttc gtg cat gtt ctt Thr Phe Val His Val Leu gtt ctt ggc tcc aac atg Val Leu G y Ser Asn IVet gca ggc aaa gtc ctt gtt Ala Gy Lys Val Leu Val act gcc ggc gcc gct gtt Thr Ala G y Ala Ala Val 75 gag gca gca ggc ctg cca Gu Ala Ala Gy Leu Pro ccttaaagcg gcg <210> 326 <211> 97 <212> PRT <213> Corynebacter i um gl <400> 326 Leu Lys G u y Val Thr 1 5 Phe Tyr Ala G n G u Thr Pro G n Thr G n Ala Val G u I e Asp G u G u Al a Asn Leu Thr Lys y Thr Ser Val y Phe Asp G u Pr o cca Pr o tgc Cys acc Thr 60 cag G n cag G n seql i st cor r ect ed. t xt gaa ggt gca cag cca caa acc caa G u G y Ala G n Pro n Thr G n 30 cac gtg cag gta gaa att gat gag His Val n Val Gu lle Asp u 45 tcc gca atc gat aac ctc acc aag Ser Ala lie Asp Asn Leu Thr Lys tgc atg aac tta agc gtt ggt ttt Cys IVet Asn Leu Ser Val y Phe 80 gt c ggc gtc gca cct taaagtagcg Val y Val Ala Pro gca Al a gaa Su gga G y gat Asp ut ari cum Al a G u G n Phe Val Hi s 25 Leu G y Ser 40 G y Lys Val 55 Al a G y Al a Al a Al a G y Al a 10 Val Asn Leu Al a Leu 90 Al a Pr o Cys Thr G n G n Val G u Hi s Ser Cys Val Tyr Sy Val Al a Met Sy G u Al a G n SIe Asn Val <210> 327 <211> 1296 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1273) <223> RXA02157 <400> 327 gggtggaatt ggcacgatgg tgctgccgga tgtttttgat cgggagaatt atcctgaagg caccgttttt agaaaagacg acaaggatgg ggaactgtaa atg agc acg ctg gaa 115 Iet Ser Thr Leu G u 1 Page 424 seql i st cor r ect ed. t xt act tgg cca cag Thr Trp Pro G n ct g Leu t ac Tyr cac Hi s cac Hi s gag Su acc Thr ttc Phe cat Hi s cca Pr o 150 t ac Tyr cca Pr o ggc G y tgc Cys gtt Val 230 ccc Pr o ggt Sy t cc Ser gac Asp gcg Al a t ca Ser at c SIle gtt Val at t SIle 120 ttc Phe aag Lys t ac Tyr gat Asp gtt Val 200 gag G u cgt Ar g gt g Val tgt Cys gt c Val aag Lys ct c Leu at c l Ie ttg Leu cgt Ar g ttc Phe cgc Ar g ggc Gy gaa G u gac Asp 170 gct Al a gca Al a ggc G y ggc G y acc Thr 250 gcc Al a att att aat acg tac Ile Ile Asn Thr Tyr gca Al a ggc G y gcg Al a gca Al a tcg Ser aac Asn act Thr acc Thr 140 ttc Phe gat Asp at c l Ie gaa G u ttg Leu 220 ttc Phe gcc Al a ggc Gy gt c Val gca Al a acc Thr agg Ar g gac Asp ggc Gy 110 cgt Ar g ggt Gy cca Pr o ttg Leu ct c Leu 190 ttc Phe at c SIle gca Al a gga G y gca Al a 270 ggc Gy gat Asp aac Asn cag G n gt c Val gcc Al a gaa G u cgg Ar g ct c Leu cca Pr o 160 aaa Lys cca Pr o aag Lys gat Asp cag G n 240 ggc G y gaa G u acc cca cca Thr Pro Pro gac cag ggc Asp n y gcg ttg ggc Al a Leu G y at c ggc caa lie G y G n gt c gag gt c Val G u Val acc ctc gcc Thr Leu Al a gca aac gag Al a Asn G u 115 att ctg gct I e Leu Al a 130 gcg ctg act Ala Leu Thr 145 agc ggt gtg Ser G y Val at g gt a gaa Mvt Val G u at c cag ggt lie n y 195 gca gtg cgc Al a Val Ar g 210 gaa gt c cag Su Val n 225 cac gat ggc Hi s Asp G y ggc ggt ctt G y G y Leu ttg atg acc Leu Iet Thr 275 gtt Val aat Asn cac Hi s ctt Leu gcc Al a gcg Al a 100 gct Al a gca Al a ggc G y gag G u acc Thr 180 gaa G u gag G u act Thr gtt Val ccc Pr o 260 cca Pr o Page 425 seql i st cor r ect ed. t xt aag cac ggc acc act ttc ggt ggc aac cca gtt gct tgt Lys His G y Thr Thr Phe G y G y Asn Pro Val Ala Cys 280 285 290 aag gca gtg ctg tct gtt gtc gat gac gct ttc tgc gca Lys Ala Val Leu Ser Val Val Asp Asp Ala Phe Cys Ala 295 300 305 cgc aag ggc gag ctg ttc aag gaa ctt ctt gcc aag gtt Arg Lys Gy G u Leu Phe Lys G u Leu Leu Ala Lys Val 310 315 320 gta gac gtc cgt ggc agg ggc ttg atg ttg ggc gtg gtg Val Asp Val Arg G y Arg G y Leu IVt Leu G y Val Val 330 335 gac gtc gca aag caa gct gtt ctt gat ggt ttt aag cac Asp Val Ala Lys G n Al a Val Leu Asp Gy Phe Lys His 345 350 ttg aat gca ccg gcg gac aac att atc cgt ttg acc ccg Leu Asn Ala Pro Ala Asp Asn lie lie Arg Leu Thr Pro 360 365 370 atc acc gac gaa gaa atc gca gac gca gtc aag gct att Ile Thr Asp u u u Ile Ala Asp Ala Val Lys Ala lie 375 380 385 atc gca taaaggactc aaacttatga ctt I e Al a 390 <210> 328 <211> 391 <212> PRT <213> Cor ynebact eri um gl ut am cum gca Al a gaa G u gac Asp ct g Leu ggc G y 355 ccg Pr o gcc Al a gct Al a gtt Val ggc G y gag G u 340 gtt Val ct g Leu gag G u gcc Al a gcc Al a gtt Val 325 cgc Ar g at t SIe gt g Val aca Thr 979 1027 1075 1123 1171 1219 1267 1296 <400> 328 Met Ser Thr Leu u Thr Trp Pro n Val Ile Ile Asn Thr 1 Thr Asp Al a SIle Val Thr Al a SIle Al a Pr o Gy Gy G n Val Al a G u 115 Al a Thr 5 G u Val Al a Gy G u G n Al a Val G n Val SIle Pr o Val Leu Ar g Lys Sy 135 Asp Sy Leu SIle Asn Lys Phe 105 Al a Hi s Ar g 10 Lys G y Al a Leu Al a G y Ile G u Al a Leu Phe Al a 75 Arg Phe Ser 90 Phe Cys Asn Arg Leu Thr G y Ar g Thr 140 G u Al a Phe Page 426 Thr SIle Val Ser Leu Ser Sy 125 eut Leu Val Al a Thr Ar g Asp Sy 110 Ar g G y Pr o Tyr G y Thr Asp Val Asn Asn n Pro Val Asp Al a Al a u Ser Arg Ser Leu IVet Pro seqI i st car r ect ed. t xt Ser Al a G31u 225 Hi s G31y Le u Al a CYS 305 Ly s ValI Ly s Thr Al a 385 ValI G31u G31y 1 95 Ar g O31n G31y Le u Thr 275 Al a G31u As p Le u G31y 355 Pr o Al a 03 u Phe 1 65 Thr As n 1 80 03 u Thr 03 u Leu Thr 03 y Val Val 245 Pr o I Ie 260 Pro G31y Al a Al a Val Al a 03 y Val 325 (31u Ar g 340 Val I Ie Leu Val Tyr Pro Tyr Gy Th r ValI As p 215 G31y As p Al a Hi s Al a 295 Ly s As p ValI As n Th r 375 As p ValI 200 (31u Ar g ValI cys G31y 280 ValI G31y ValI Al a Al a 360 ValI 1 85 Pr o Ty r Th r ValI Le u 265 Th r Le u (31u Ar g Ly s 345 Pr o As p 1 70 Al a Al a G31y G31y Th r 250 Al a Th r Ser Le u G31y 330 O31n Al a 1 Thr Asp Tyr Leu Arg 1 Al a I I e Phe Leu 03 u 1 Pr o O31u 31y Phe Leu 205 li1e Leu I'b t I Ie Thr 220 As p Phe Phe Al a Hi s 235 I'b t Al a Lys 03 y Leu 255 Thr 31y Ar g Al a Al a 270 Phe 31y G31y As n Pr o 285 Val Val As p As p Al a 300 Phe Lys 03 u Leu Leu 315 Ar g G31y Leu I'b t Leu 335 Al a Val Leu As p G31y 350 As p As n I Ie I Ie Ar g 365 Asp 0 u 0 u Il e Al a 380 Asp Al a Val Lys O31u Th r I Ie Al a 390 <210O> 329 <21 1> 1491 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221 OIDS <222> (1468) <223> RXS02970 <400> 329 aaccgacaaa acagccgt t c acgt gct aaa gcagct cggc t t gat ct agg gt gaggt gag t tat t taaag act tcat aat att t tgggga gt gaact ggt t tg gca t tg aag ggt 115 Leu Al a Leu Lys 03 y 1 t ac acc aac t tt gac ggt gaat t cat cgaat t cgga tct gt gcaa gca 163 Tyr Thr Asn Phe Asp Gy Ou Phe Ilie u Phe (31y Se r Val (31n Al a 15 Page 427 seql i st cor r ect ed. t xt aaa Lys cac Hi s gcc Al a at g Met tta Leu ccg Pr o tcg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr t ca Ser cac Hi s at c l Ie 230 gca Al a ct c Leu ct g Leu gag G u tgg Tr p ggt Sy t cc Ser gag Su ttc Phe gcc Al a 120 gcc Al a aaa Lys at g Met cca Pr o ttt Phe 200 gaa G u ct g Leu t ac Tyr at c SIle gct Al a 280 aaa cgg gca Lys Arg Ala gcg Al a acg Thr ctt Leu at c SIle aat Asn ggc Gy gag G u ct g Leu ct c Leu 170 at c SIle acc Thr gt c Val cca Pr o aat Asn 250 gac Asp gag G u cag G n ct g Leu gt c Val 75 cag G n gat Asp gaa G u cac Hi s t cc Ser 155 acc Thr tac Ty r acc Thr at c SIle gt g Val 235 ggc G y gaa G u cat Hi s gac Asp t ac Tyr tcg Ser cgc Ar g gt g Val ttc Phe t cc Ser 140 gca Al a ggc Gy cac Hi s caa G n gcg Al a 220 gt g Val gt g Val gt c Val gct Al a ttc gac aac Phe Asp Asn aaa atc agc Lys Ile Ser 45 gac ttc gac Asp Phe Asp gca aac tta Al a Asn Leu caa gca gcc G n Ala Al a 95 cgc t ct gat Arg Ser Asp 110 t cc cac gt g Ser Hi s Val 125 atc cgc at g lie Arg IVet tac cgc agc Tyr Arg Ser gaa cac cgc G u His Ar g 175 ttc tgg gca Phe Trp Al a 190 gaa gaa gaa Su G u u 205 ttt gaa ggt Phe G u G y gga t ca t ca G y Ser Ser cgc gaa ctt Arg G u Leu 255 at g gt c gga Mvt Val G y 270 ggc gac gat G y Asp Asp 285 gat Asp ccc Pr o ggc G y ggc G y 80 cgg Ar g gtt Val ttt Phe gct Al a t ac Tyr 160 cgc Ar g cca Pr o tgc Cys gct Al a gga G y 240 tgc Cys ttc Phe ttc Phe cgc gcg Arg Ala aaa gt a Lys Val aac gcc Asn Al a cac aac His Asn ttg acc Leu Thr gct gca Al a Al a ttc acc Phe Thr 130 cgc ctg Arg Leu 145 cac ggc Hi s G y ctg ggc Leu G y ttc ctg Phe Leu gaa cgc G u Arg 210 ggc at g G y tt 225 at c at c Ile Ile aac aag Asn Lys gga cgc G y Arg cag cca G n Pro 290 cac gtt His Val tgg gca Trp Al a ttc atc Phe Il e aac cct Asn Pro aac at c Asn Il e 100 aag at c Lys II e 115 aac ggc Asn y cac acc Hi s Thr gca acc Al a Thr aac ccc Asn Pro 180 cac cac Hi s Hi s 195 gca ct c Al a Leu atc gca I e Al a ctg cca Leu Pro cac ggc Hi s G y 260 acc gga Thr G y 275 gac atg Asp I\et ttc Phe gct Al a gac Asp cga Ar g aac Asn gtg Val ggc gay gga Gy gga Gy 165 acc Thr t cc Ser aag Lys gcg Al a cca Pr o 245 at c SIe aaa Lys at c SIe Page 428 seql i st cor r ect ed. t xt acc ttc gcc aag ggt gtt aac gca ggt t Thr Phe Ala Lys y Val Asn Ala y T 295 300 gtg atg acc caa tca atc cgc gat acc t Val bet Thr G n Ser Ile Arg Asp Thr PI 310 315 ggc gga ctc acc tac tcc gga cac cca c Sy y Leu Thr Tyr Ser y His Pro L 330 3 gca gcg ctg gag att tac gcg gaa gga g Ala Ala Leu G u Ile Tyr Ala G u G y G 345 350 cga ctt ggc gct gaa ctg atc gaa cct c Arg Leu G y Ala u Leu lie u Pro Ai 360 365 gaa aac gta gcg atc gct gac gtg cgg g Su Asn Val Ala lle Ala Asp Val Arg G 375 380 gtg gag ttc aat gca gac gcc act gcc al Val G u Phe Asn Ala Asp Ala Thr Ala M 390 395 ttc aag gaa cgc ggc gtg tgg ccg atg al Phe Lys G u Arg G y Val Trp Pro IVet II 410 4 atc gcg ccg ccg ctg acc acc act gat g, lie Ala Pro Pro Leu Thr Thr Thr Asp A 425 430 gac gcg gtg gaa gct gca gcc caa gct gl Asp Ala Val G u Ala Al a Ala G n Ala V 440 445 gcg ttg ttc taagttttct agataacaag gcc Ala Leu Phe 455 <210> 330 <211> 456 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 330 Leu Al a Leu Lys G y Tyr Thr Asn Phe A 1 5 Sy Ser Val n Ala Lys u u u L Arg Ala His Val Phe His Ser Trp Ser Al 40 Lys Val Trp Ala Ala Al a G u Gy Ser TI 55 Asn Ala Phe Ile Asp IVet Gy Ser n Li 70 His Asn Asn Pro Arg Leu Val G u Ala II P ac gcc yr Al a tc gga he G y 320 tt gca eu Al a 35 ag at c u Ile gc ctt rg Leu gc at c y Ile tg gct et Al a 400 tc t cc e Ser 15 ac gaa sp G u tc gag al G u cca ct c ggt Pro Leu G y 305 t ca gag gca Ser G u Al a gt a gca ccc Val Ala Pro att cca cgc I e Pro Ar g 355 cgt gaa ct a Arg G u Leu 370 gga ttc ttc G y Phe Phe 385 gcc ggt gct Al a G y Al a ggc aac cga G y Asn Ar g ttg gta gca Leu Val Al a 435 ctg acc ttc Leu Thr Phe ggc G y t ac Tyr gcc Al a 340 gt a Val gcg Al a tgg Tr p gca Al a ttc Phe 420 ct g Leu gct Al a G u Asn Ser Asp Leu Al a at c SIe t cc Ser 325 aag Lys gct Al a gaa G u gca Al a gaa G u 405 cac Hi s ct g Leu ggg G y 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1491 sp G y 10 ys Arg a G n hr Leu eu Val e G n age 429 G u Al a Asp Tyr Ser Ar g 450 Phe Phe Lys Asp Al a G n seqi i st car r ect ed. t xt As n Pr o Ala Ph e Asn Asp Val Le u A a Ph e Ar g 145 Hi s Le u Ph e O31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI I Ie Ar g G31y 385 A a G31y Le u As n Ly s 115 As n Hi s A a As n Hi s 1 95 A a I Ie Le u Hi s Thr 275 As p G31y A a Pr o Ar g 355 Le u Ph e A a Ar g A a 435 ValI G31y G31y G31y 1 65 Thr Ser Ly s A a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s A a (31u A a (31u 405 Hi s Le u Ser A a Ar g 150 Ser Thr Ser Hi s I Ie 230 A a Le u Le u Thr ValI 310 G31y A a Ar g (31u ValI 390 Ph e I Ie As p As p 135 As n A a As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y A a Le u As n 375 (31u Ly s A a A a A a 120 A a Ly s vb t Pr o Ph e 200 (31u Le u Tyr I Ie A a 280 A a Thr Le u Le u G31y 360 ValI Ph e (31u Pr a ValI 440 G31y 1 05 Ar g le le vb t As p 1 85 A a As p O31u Le u A a 265 Tyr Ly s O31n Thr O31u 345 A a A a As n Ar g Pr a 425 O31u (31u Hi s Ser 1 55 Thr Tyr Thr I Ie ValI 235 G31y O31u Hi s ValI I Ie 315 Ser Tyr Le u A a As p 395 ValI Thr Ph e Ser 140 A a G31y Hi s O31n A a 220 ValI ValI ValI A a As n 300 Ar g G31y A a I Ie As p 380 A a Tr p Thr Ar g Ser 110 Ser Hi s 125 li1e Ar g Ty r Ar g O3 u Hi s Phe Tr p 1 O31u (1u 205 Phe 03 u O3 y Ser Ar g O31u [Vb t Val 270 31y As p 285 A a 03 y Asp Thr Hi s Pr o O31u (1y 350 O31u Pr o 365 Val Ar g Thr A a Pr o lb t Thr As p 430 Asp Val ValI vb t Ser Ar g 1 A a O31u G31y Ser Le u 255 G31y As p Tyr Ph e Le u 335 O31u Ar g G31y vb t I Ie 415 As p Ph e A a Tyr 1 Ar g Pr a cys A a G31y 240 cys Ph e Ph e A a G31y 320 A a I Ie Le u I Ie A a 400 Se r O31u Ala Ala Ala O31n Ala Val O31u 445 Page 430 seal i st car r ect ed. t xt Leu Thr Phe Ala 3y Ala Leu Phe 450 455 <210> 331 <211> 1330 <212> DNA <213> Cor ynebact er i ur gl ut ani cum <220> <221> CDS <222> (101)..(1330) <223> FRXA01009 <400> 331 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttg ttatttaaag acttcataat attttgggga gtgaactggt ttg Leu at ct agg gt gaggt gag gca Al a t ac Tyr aaa Lys cac Hi s gcc Al a at g Ivb t tta Leu ccg Pr a t cg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr aac Asn gag (31u t gg Tr p ggt G31y t cc Ser gag (31u ttc Phe gcc Al a 120 gcc Al a aaa Lys at g Ivb t cca Pr a gac Asp aaa Lys gcg Al a acg Thr ct t Leu at c SI e aat Asn ggc G31y gag (31u ct g Leu ct C Leu 170 at c SI e g aa (31u gca Al a gac Asp t ac Tyr t cg Ser cgc Ar g gt g Val ttc Phe t cc Ser 140 gca Al a ggc G31y cac Hi s ttc Phe ttc Phe aaa Lys 45 gac Asp gca Al a caa O31n cgc Ar g t cc Ser 125 at c SI e t ac Tyr g aa G31u ttc Phe at c SI e gac Asp at c SI e ttc Phe aac Asn gca Al a t ct Ser 110 cac Hi s cgc Ar g cgc Ar g cac Hi s t gg Tr p 190 g aa G31u 15 aac Asn agc Ser gac Asp tta Leu gcc Al a 95 gat Asp gt g Val at g Ivb t agc Ser cgc Ar g 175 gca Al a ttc Phe gat Asp ccc Pr a ggc G31y ggc G31y 80 Cgg Ar g gt t Val ttt Phe gct Al a t ac Tyr 160 cgc Ar g cca Pr a 1 gga G31y cgc Ar g aaa Lys aac Asn Cac Hi s ttg Leu gct Al a ttc Phe cgc Ar g 145 Cac Hi s ct g Leu ttc Phe t ct Ser gcg Al a gt a Val gcc Al a aac Asn acc Thr gca Al a acc Thr 130 ct g Leu ggc G31y ggc G31y ct g Leu ttg aag ggt Leu Lys 0 y gt g caa gca Val On Ala cac gtt ttc Hi s Val Phe t gg gca gct Trp Ala Al a ttc atc gac Phe Ile Asp aac cct cga Asn Pr o Ar g aac atc aac Asn Ile Asn 100 aag at c gt g Lys Ile Val 115 aac ggc ggc Asn y3y cac acc gga Hi s Thr 0 y gca acc gga Al a Thr 0 y 165 aac ccc acc Asn Pr o Thr 180 cac cac tcc Hi s Hi s Ser 195 Page 431 seql i st cor r ect ed. t xt tca ttc ttt Ser Phe Phe 200 cac tt g gaa Hi s Leu G u 215 at c gt c ct g Ile Val Leu 230 gca ggt t ac Al a G y Tyr ctc ttc atc Leu Phe Il e ctg ttt gct Leu Phe Al a 280 acc tt c gcc Thr Phe Al a 295 gtg atg acc Val Met Thr 310 ggc gga ct c G y G y Leu gca gcg ct g Al a Al a Leu cga ct t ggc Arg Leu G y 360 gaa aac gt a G u Asn Val 375 gtg gag ttc Val G u Phe 390 ttc aag gaa Phe Lys G u gcc acc acc caa Ala Thr Thr G n gt c Val cca Pr o aat Asn 250 gac Asp gag G u ggt G y t ca Ser t ac Tyr 330 at t SIle gaa G u at c SIle gca Al a ggc G y 410 at c SIle gt g Val 235 ggc G y gaa G u cat Hi s gtt Val at c SIle 315 t cc Ser tac Ty r ct g Leu gct Al a gac Asp 395 gcg Al a 220 gt g Val gt g Val gt c Val gct Al a aac Asn 300 cgc Ar g gga G y gcg Al a at c SIle gac Asp 380 gaa gaa gaa Su u u 205 ttt gaa ggt Phe G u G y gga t ca t ca G y Ser Ser cgc gaa ctt Arg G u Leu 255 at g gt c gga Mvt Val G y 270 ggc gac gat G y Asp Asp 285 gca ggt tac Al a G y Tyr gat acc ttc Asp Thr Phe cac cca ctt His Pro Leu 335 gaa gga gag Su y u 350 gaa cct cgc G u Pro Arg 365 gtg cgg ggc Val Arg G y tgc gaa Cys u gct Al a gga G y 240 tgc Cys ttc Phe ttc Phe gcc Al a gga G y 320 gca Al a at c SIle ctt Leu at c SIle ggc G y 225 at c SIle aac Asn gga G y cag G n cca Pr o 305 t ca Ser gt a Val att SIle cgt Ar g gga G y 385 cgc gca Arg Ala 210 at g at c 'e t Ile at c ct g I e Leu aag cac Lys Hi s cgc acc Arg Thr 275 cca gac Pro Asp 290 ctc ggt Leu G y gag gca G u Al a gca ccc Ala Pr o cca cgc Pro Ar g 355 gaa ct a G u Leu 370 ttc ttc Phe Phe ct c Leu gca Al a cca Pr o ggc G y 260 gga G y at g Met ggc G y t ac Tyr gcc Al a 340 gt a Val gcg Al a tgg Tr p aag Lys gcg Al a cca Pr o 245 at c SIe aaa Lys at c SIe at c SIe t cc Ser 325 aag Lys gct Al a gaa G u gca Al a 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1330 gcc act gcc atg gct gcc ggt gct gca gaa Ala Thr Ala bet Ala Ala y Ala Ala u 400 405 <210> 332 <211> 410 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 332 Leu Al a Leu Lys G y Tyr Thr Asn Phe Asp G y G u Phe Ile G u Phe 1 5 10 Gy Ser Val n Ala Lys G u u G u Lys Arg Ala Phe Asp Asn Asp Page 432 seqI i st car r ect ed. t xt Ar g Ly s As n Hi s Le u A a Ph e Ar g 145 Hi s Le u Ph e O31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI I Ie Ar g Hi s Tr p Ph e As n As n Ly s 115 As n Hi s A a As n Hi s 1 95 A a I Ie Le u Hi s Thr 275 As p G31y A a Pr o Ar g 355 Le u Ph e A a As p Ar g As n ValI G31y G31y G31y 1 65 Thr Ser Ly s A a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s A a (31u Hi s A a vb t 70 Le u Pr o Ser A a Ar g 150 Ser Thr Ser Hi s I Ie 230 A a Le u Le u Thr ValI 310 G31y A a Ar g (31u Ser (31u G31y ValI A a Nibt As p 135 As n A a As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y A a Le u As n 375 Tr p Ser 40 03 y Ser Ser 03 n 03 u A a Phe 03 y 1 Ala Ar g 120 A a I I e Lys I I e Ivb t b t Pr o As p 1 Phe A a 200 31u As p Leu 03 u Tyr Leu I e A a 265 A a Ty r 280 A a Lys Thr 03 n Leu Thr Leu 03 u 345 03 y A a 360 Val Ala li1e Ser Pro A a 03 n As p Le u ValI 75 O31n As p O31u Hi s Ser 1 55 Thr Tyr Thr I Ie ValI 235 G31y O31u Hi s ValI I Ie 315 Ser Tyr Le u Tyr Ser Ar g ValI Ph e Ser 140 A a G31y Hi s O31n A a 220 ValI ValI ValI A a As n 300 Ar g G31y A a I Ie Ly s As p A a O31n Ar g Ser 125 li1e Tyr O31u Ph e O31u 205 Ph e O31y Ar g vb t O31y 285 A a As p Hi s O31u O31u 365 As p Le u A a As p ValI vb t Ser Ar g 1 A a O31u O31y Ser Le u 255 O31y As p Tyr Ph e Le u 335 O31u Ar g (31y O31y Ar g ValI Ph e A a Tyr 1 Ar g Pr a cys A a O31y 240 cys Ph e Ph e A a O31y 320 A a I Ie Le u I I e A a Asp 380 Val Arg O1y Ilie Page 433 seql i st cor r ect ed. t xt Sy Phe Phe Trp Ala Val u Phe Asn Ala Asp Ala Thr Ala lMt Al a 385 390 395 400 Ala G y Ala Ala G u Phe Lys u Arg y 405 410 <210> 333 <211> 1080 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1057) <223> RXA02158 <400> 333 aacattatcc gtttgacccc gccgctggtg atcaccgacg aaggctattg ccgagacaat cgcataaagg actcaaactt aagaaatcgc agacgcagtc atg act tca caa cca 11! lMt Thr Ser G n Pro cag G n gca Al a gag G u act Thr ggt Sy ggc Sy gca Al a gag G u cca Pr o cct Pr o 150 ggc Sy cgc Ar g gtt Val cca Pr o act Thr cac Hi s t cc Ser gt g Val t cc Ser 120 cag G n gaa G u ggc Gy ttt Phe acc Thr gag G u act Thr at c SIle cag G n cgc Ar g gt g Val ct g Leu cca Pr o aac Asn 170 ct g Leu ct a Leu gga Gy cgc Ar g gt c Val 75 gac Asp acc Thr ccg Pr o gct Al a gca Al a 155 aac Asn gct Al a gcc Al a cca Pr o ttc Phe gt g Val acc Thr t ac Tyr ct g Leu gat Asp 140 ggc Gy at g Met gat Asp gca Al a aag Lys 45 t cc Ser gat Asp gca Al a gca Al a gt g Val 125 ct g Leu ctt Leu gcc Al a gat Asp aag Lys t cc Ser ttc Phe t cc Ser gct Al a cac Hi s 110 aac Asn cag G n aag Lys aac Asn ct c Leu aag Lys gca Al a gcg Al a agc Ser ttg Leu aat Asn ttg Leu at c SIle aag Lys 160 t ac Tyr cct Pr o gcg Al a ctt Leu at c l Ie cag G n cgc Ar g cac Hi s gat Asp 130 gaa G u gct Al a at t SIle gca Al a ccg Pr o ttt Phe gct Al a at g Met t ac Tyr gcc Al a 115 gat Asp aac Asn gt g Val ggc Gy gag Gu ttt Phe gat Asp cat Hi s ggt Sy gtg Val 100 at g let ctg Leu ct c Leu t ac Tyr ttt Phe 180 Page 434 seqI i st car r ect ed. t xt acc gcg ggc at g gat Thr A a 03 y I'bt Asp 1 85 at t t cc at c at c act cct gaa ggg t tc cag cct Pro Ou Gy Phe O1n Pro IlieSer Ilie Ilie cgt Ar g gcg A a gt t ValI 230 at c I Ie at g vb t t ac Ty r aaa Ly s g aa G31u 200 gt t ValI at c I Ie cgc Ar g aaa Ly s ggc (31y 280 tt c Ph e gt g ValI gt c ValI g at As p ac a Th r 250 aac As n g aa G u g aa 0Ou gag G31u acc Th r acc Th r 235 cct Pr o gac As p gt g ValI gca A a cgc Ar g g ac As p 220 t gg Tr p tt c Ph e ggc G31y gca A a g aa O31u 300 gcg A a 205 ag c Ser gt a ValI gt t ValI gcc A a gcc A a 285 aac As n 1 90 g aa G31u ct c Le u t cc Ser cct Pr o at c I Ie 270 t cc Ser cgc Ar g cgt Ar g gag O31u ggt G31y 240 c ag O31n ct g Le u at t I Ie c ac Hi s 1 ggc cag gaa (31y (1n (1u 210 gtt gcc ggc Val Ala G31y 225 atg gaa aac [Vb t O31uAsn gt c aac gat Val Asn Asp cac t gc ct t Hi s Cys Leu 275 gat gga cca Asp G31y Pr o 290 gct cag aaa A a 03 n Lys acc ggc Thr 03 y gcc gat A a As p gac ggc As p (1y 245 gag gt C O3 u Val 260 cct gcc Pr o Ala gcg t cc A a Ser gca ct g A a Leu 691 739 787 835 883 931 979 1 027 1 077 1 080 305 ct g gt g t gg ct g ct g gcc aac cag ccg agg Leu Val Trp Leu Leu A a Asn O31n Pro Arg 310 315 aac <210> 334 <211> 319 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 334 I'bt Thr Ser O31n Pro On Val Arg His Phe 1 5 10 Thr Pr o Ala (1u (1n Ala (1u VaI Leu Thr 25 A a A a Pro Ph e Ser 03 u Ar g Pr o Leu 03 u 40 Val Leu Phe Asp Lys Thr Ser Thr Arg Thr 55 O3 y Il e A a Hi s Leu 03 y 03 y Hi s A a I I e 70 Ser O31n 'b t O31y Lys O31y O31u Se r Leu O31n 90 Ser Ar g Tyr Val O31u Ala I Ie Val Trp Arg 100 105 Phe Hi s A a N/t A a 03 u Thr Ser Thr Val 115 120 Pag t aagacat gt ccct t ggct c Leu Leu 31yI Ar gI Val As p Thr Pr oI e 435 A a A a Pr o Ph e ValI Th r Ty r e u As p A a Lys5 Ser As p A a A a ValI 125 As p Lys5 Ser Ph e Ser A a Hi s 110 As n As p Le u ValI As p O31y ValI Se r Se r seqI i st car r ect ed. t xt Ser As p 130 Val 0Ou 145 Lys A a I'b t I Ie 0Ou 0y 0Gy 0n 210 Val Ala 225 [vb t 0u Val As n Hi s Cys As p 0y 290 A a 0 n 305 Asp Leu Hi s Pro As n ValI 0Gy Ph e 1 95 0Ou 0Gy As n As p Le u 275 Pr o Lys5 Ser Le u 1 65 A a Pr o 0Gy As p 0Gy 245 ValI A a Ser Le u Cys 135 0Ou As p A a A a Lys5 215 ValI As p A a Ar g ValI 295 ValI O n Il e Leu Al a 0Gy As p vb t 1 Ph e ValI Thr Thr A a 265 Lys5 As p Le u A a 1 As n I Ie 0Ou Thr Thr 235 Pr o As p ValI A a A a 315 As p 140 0Gy vb t Ser Ar g As p 220 Tr p Ph e G y A a 0Ou 300 As n Leu On Thr Ilie Le u A a I Ie A a 205 Ser ValI ValI A a A a 285 As n O n 0Gy Ser 1 A a As p vb t Tyr 255 Ph e ValI Le u Ar g <21 0> 335 <21 1> 1326 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 OIDS <222> (1303) <223> RXA02160 <400> 335 tt t tcgagga at accaaccc t t tcaacaca at aatt t tct ccacggctgg caaggaactt aaaatgaagg agcacacctc gt t ValI ct g Le u ggc 0Gy gcc gca A a aag Lys5 ggt 0Gy gca t cc Ser at t I Ie gag 0Ou gag ggt 0Gy ggt 0Gy at g vb t at c ct g Le u g aa 0Ou g ac As p 45 gt t g ac As p gt c ValI aac As n gt t acc act Thr Thr 15 at c gca I e A a gt t cgc Val Ar g gat gca Page 43E t taaacat cc t tgct gt cca at g act aac cgc at c I'b t Thr Asn Arg I Ie 1 gt g gca at t cca t ac Val Ala I Ie Pr o Ty r gt t t cc ct c gac ct g Val Ser Leu As p Leu cag cgt gca t tg gat O n Ar g Ala Leu As p aag gat gag t tc gct Al a G3 y Ala Ala Ou Ser Ilie seqI i st car r ect ed. t xt Val Val Asp Al a Lys Asp G3 u Phe Al a gag O31u c ag O31n ct c Le u t gc cys g ac As p t gg Tr p 1 50 at c I Ie ggC G31y ac c Thr cca Pr o g at As p 230 cgt Ar g ct c Le u gca Al a g aa O31u gt a ValI 310 t ac Tyr cca Pr o gag O31u ggt G31y 120 g at As p cgc Ar g c ag O31n gct Al a g ac As p 200 gag O31u cgt Ar g 99C G31y 99C G31y at t I Ie 280 gct Al a g ac As p ct g Le u gt t ValI 99C G31y 99C G31y aac As n aag Ly s gt g ValI 1 70 gag O31u t ac Tyr at c I Ie gt c ValI c ag O31n 250 aag Ly s gct Al a t ac Tyr ct g Le u cca Pr o 75 t ct Ser aag Ly s aac As n ct g Le u gct Al a 155 aag Ly s acc Thr gca Al a at c I Ie t cc Ser 235 ggC G31y t cc Ser cac Hi s aag Ly s t gg Tr p 315 ac c Thr gca Al a c ag O31n g ac As p gag O31u 140 at c I Ie t cc Ser ggt O31y t ac Tyr t cc Ser 220 gt a ValI gt t ValI cgc Ar g gag O31u cgc Ar g 300 tt c Ph e at c I Ie at c I Ie tt c Ph e c ag O31n 125 at c I Ie gcc Al a cca Pr o t ac Tyr ac c Thr 205 tt c Ph e ct g Le u ggC O31y g aa O31u gct Al a 285 ggC O31y gga O31y aag Ly s t cc Ser aac As n 110 gt t ValI at t I Ie tt c Ph e tt c Ph e ct g Le u 1 90 gag O31u gag O31u c ag O31n cgc Ar g at c I Ie 270 tt g Le u gt t ValI cct Pr o gca Al a cgc Ar g 95 ggt O31y cgt Ar g gca Al a gcc Al a t cc Ser 1 75 g aa O31u g at As p ggt O31y gct Al a ct t Le u 255 t ac Tyr g aa O31u g ac As p ct g Le u aac As n 80 cca Pr a ac c Thr tt c Ph e cct Pr a gag O31u 1 60 at c I Ie g at As p cca Pr a O31y at t I Ie 240 g ac As p g aa O31u g at As p gca Al a aag Ly s 320 ggC O31y ct g Le u c ac Hi s gag O31u gct Al a 145 gag O31u g ac As p ct g Le u gct Al a aag Ly s 225 g aa O31u at g vb t gca Al a gt c ValI cgt Ar g 305 cgc Ar g at g vb t at c I Ie gt t ValI gt c ValI 130 cgt Ar g aac As n c ag O31n t gg Tr p ct g Le u 210 cca Pr a gag O31u gt t ValI cca Pr a ac c Thr 290 t gg Tr p t cc Ser t ac Tyr gt c ValI gca Al a 115 ggC O31y g ac As p aac As n aac As n aat As n 1 ggt O31y gt c ValI ct g Le u gag O31u O31y 275 at c I Ie gct Al a ct g Le u at g vb t aag Ly s 1 00 c ac Hi s tt c Ph e tt c Ph e gt t ValI gt c ValI 1 gct Al a aac As n t cc Ser aac As n g ac As p 260 gca Al a gag O31u gag O31u g ac As p aag Ly s c ac Hi s ggC O31y at g vb t gca Al a cca Pr a 1 t gg Tr p cca Pr a gct Al a at c I Ie cgt Ar g 245 cgt Ar g at c I Ie cgc Ar g g aa O31u gcg Al a 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 t t cat t gat t cc acc cag gag cac gt cacc ggc gat at ccgc at ggt t Page 437 Phe I I e Asp Ser Thr G3 n G3 u Hi s\ 330 ct g cac gca ggt t cc at c acc at c a Leu Hi s Al a O31y Ser I Ie Thr I IeA 3453 ct g t ac gac t tc aac ct g gct acc t Leu Ty r As p Phe As n Leu Al a Thr T 360 365 cag acc ct g gct aag ggc t tt gt c c O31n Thr Leu Al a Lys G31y Phe Val C 375 380 at c gct aac aag cgc gat cgc gaa g Ilie Al a Asn Lys Ar g Asp Ar g a3 u P 390 395 t t tcaagcat cca <210> 336 <211> 401 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ami cumn I i st car r ect ed. t xt (al Thr Gy Asp IlieArg I'b t Val 335 340 at ggt cgt cgt t cc agc cac t cc osn G31y Ar g Ar g Ser Ser Hi s Ser 50 355 ac gac acc ggc gac acc ttc gac yr Asp Thr G31y Asp Thr Phe Asp 370 ag ct g cac ggt ct g t cc t cc aag ~n Leu Hi s G31y Leu Ser Ser Lys 385 ct ggc aac aac t aagccacct Il a 03 y Asn Asn 400 1171 1219 1267 1313 1326 <400> 336 I'bt Thr Asn ValI ValI O31n G31y Le u Hi s O31u Al a 145 O31u As p Le u I Ie Le u Al a O31u Tyr ValI Al a 115 G31y As p As n As n As n 1 95 Ar g I Ie Val Pr o Tyr Leu Asp Leu 03 y Leu Asp Al a Phe Al a 03 u 70 I'b t Lys 03 n Lys His5 Leu 1 00 Hi s 03 y Cys Phe I'b t Asp Phe Al a Tr p 150 Val Pr o Ilie 1 65 Val Tr p G31y 1 80 Al a Pr o Thr Le u Lys5 O31n G31y (31u Tyr ValI Thr Thr 135 Thr (31u Ar g Lys5 Al a Lys5 G31y 40 Al a Tyr Pr o (31u G31y 120 As p Ar g O31n Al a As p 200 Tyr vb t O31y Al a Cys Le u Al a 1 Ly s Pr o As p Ser I Ie 1 li1e O31y As p As n Ser Pr o Ser Ly s As n Le u Al a 1 Ly s Thr Al a Le u O31u As p ValI I Ie I Ie Ph e O31n 125 li1e Al a Pr o Tyr Thr 205 Thr lie ValI As p Al a Ar g O31y Ar g Al a Al a Se r 1 O31u As p Aa Leu OyAsnAia ProAspOuVa Ilie IleSer Phe3u Oy Oy Page 438 seqi i st car r ect ed. t xt 215 220 Val Leu O31n Ala Ly s 225 G31u vb t A a ValI Ar g 305 Ar g As p Ar g G31y G31y 385 As n ValI Le u G31u G31y 275 li1e A a Le u Ar g Ser 355 Thr Ser I Ie Ar g 245 Ar g I Ie Ar g G31u A a 325 ValI Ser As p Ly s G31y Ar g Pr o Val A a ValI Le u Le u 295 Tyr I Ie Hi s Tyr Thr 375 A a G31y Gy le 280 A a As p As p A a As p 360 Le u As n A a I Ie 265 Ly s Ar g G31y Ser G31y 345 Ph e A a Ly s Ser 235 G31y Ser Hi s Ly s Tr p 315 O31n I Ie Le u G31y As p 395 ValI Ar g O31u Ar g 300 Ph e O31u Thr A a Ph e 380 Ar g G31y O31u A a 285 G31y G31y Hi s I Ie Thr 365 ValI (31u Le u 255 Tyr O31u As p Le u Thr 335 G31y As p Le u G31y I Ie 240 As p O31u As p A a Ly s 320 G31y Ar g Thr Hi s As n 400 <210O> 337 <21 1> 1554 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1531) <223> RXN02162 <400> 337 gat cgct aac aagcgcgat c gcgaagct gg caacaact aa gact agaact t caagt at tt agaaagt aga agaacaccac acc aat gaa ggt gcg ct g t gg ggc ggc Thr As n 03 u 03 y A a Leu Tr p 03 y 03 y gag gcc at g t tc gcc t tg agt gt c t cc O31u Ala l'b t Phe Ala Leu Ser Val Ser 30 gcc cct t at gat gt g t tg gcc t cc aag Ala Pr o Tyr Asp Val Leu Ala Ser Lys 45 cgc tt c Ar g Phe 15 act cat Thr Hi s gca cac A a Hi s Page 43 gccacct t t t caagcat cca atg gaa cag cac gga l'b t 31u O31n Hi s G31y 1 t cc ggt gga ccc t cc Ser G31y G31y Pr o Ser t tc gac t gg gt t tt g Phe Asp Trp Val Leu gcc aag gt t t tg cac A a Lys Val Leu Hi s caa gca gat cta ctt tct G n Ala Asp Leu Leu Ser ctt Leu cct Pr o cgc Ar g aac Asn cgc Ar g caa G n 150 cag G n cag G n ctt Leu aag Lys gca Al a 230 acc Thr gaa G u tct Ser cct Pr o aac Asn 310 cag G n gat Asp ggt Gy cag G n 120 at c SIle aag Lys gct Al a ttg Leu gt g Val 200 aac Asn aac Asn ttc Phe at c SIle t cc Ser 280 gt g Val acc Thr ggc Gy gat Asp gag G u gca Al a ct g Leu cat Hi s ccg Pr o 170 cgc Ar g cct Pr o gaa G u at t SIle ct g Leu 250 gca Al a t cc Ser gag G u ct g Leu aag Lys 75 gt g Val gt g Val acc Thr gga Gy gca Al a 155 gt c Val gat Asp tac Tyr gca Al a gat Asp 235 gcg Al a tgg Tr p aca Thr ct g Leu ct g Leu 315 gat Asp gat Asp cac Hi s ggc Gy ct g Leu aca Thr 140 ggc Gy ctt Leu at t SIle ggt Gy at c l Ie 220 gcc Al a cag G n tgc Cys ggc G y acc Thr 300 gct Al a gt c Val ggc G y ggc G y ttc Phe 125 acc Thr gcg Al a ct g Leu gat Asp t cc Ser 205 gct Al a acc Thr ctt Leu acc Thr agc Ser 285 cgt Ar g acc Thr gcc Al a gcg Al a cgt Ar g 110 cgc Ar g gag G u at c l Ie gca Al a cgt Ar g 190 ggc Sy gaa G u agc Ser gca Al a cca Pr o 270 t ca Ser ggc G y ct g Leu seql i st cor r ect ed. t xt gaa gat cta gcc acc atg ctg gct ggg G u Asp Leu Ala Thr IVet Leu Ala y gac Asp at g Met 95 ct g Leu at g Met ctt Leu at g Met cac Hi s 175 at c SIle gca Al a gaa G u t cc Ser gt g Val 255 gaa G u at c SIle aag Lys aag Lys gga Gy 80 gaa G u cgc Ar g tgg Tr p gt c Val cca Pr o 160 cag G n cgt Ar g ctt Leu ct c Leu cgc Ar g 240 gat Asp ttt Phe at g Met tct Ser gca Al a 320 acc Thr cgc Ar g gct Al a gt c Val gac Asp 145 ggc Gy ct g Leu gac Asp gct Al a ggc Sy 225 gat Asp at g let ggt G y ccg Pr o ggt 35y 305 ttc Phe ggt G y ggt G y cgc Ar g 130 gcc Al a aag Lys ct g Leu ct g Leu ggt Sy 210 ttt Phe ttc Phe t cc Ser t ac Tyr cag G n 290 cgc Ar g ccg Pr o at t SIle 100 t cc Ser gca Al a agc Ser cac Hi s cac Hi s 180 aag Lys tct Ser t cc Ser tct Ser ttg Leu 260 acc Thr aag Lys at c SIle ct g Leu gac Asp cgc Ar g gt g Val gcc Al a ttc Phe 165 gca Al a cgt Ar g ttg Leu gca Al a gaa G u 245 gct Al a ttg Leu aac Asn ggt G y t ac Tyr 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 cag cct tta gcg Sn Pro Leu Ala Page 440 aac cgc Asn Arg gac ctg cag gaa gat Asp Leu G n G u Asp 330 seql i st cor r ect ed. t xt aag gaa cca atc gta gat tcc gtg gcg Lys u Pro lie Val Asp Ser Val Ala cag ctc aac ctg ctg ctc cct gca G n Leu Asn Leu Leu Leu Pro Ala 345 acc ttc aac acc gag cgc atg cgt Thr Phe Asn Thr G u Arg Iet Arg 360 365 ctt gcc acc gac ttg gct gag tgg Leu Ala Thr Asp Leu Al a u Trp 375 380 cgt gag gca cac gaa gca tcc ggc Arg G u Ala His G u Ala Ser G y 390 395 agg gga gtg gac ctt atc gat ctc Arg y Val Asp Leu Ile Asp Leu 410 gat gca cgt ctg acc cca gag gta Asp Ala Arg Leu Thr Pro G u Val 425 gca gtg gct tcc cgt gca acg cgc Ala Val Ala Ser Arg Al a Thr Arg 440 445 gcg gag caa cgc gca cgt gtc gat Ala G u G n Arg Ala Arg Val Asp 455 460 tgg gca cgt gcg ggg gta cgt cga Trp Ala Arg Ala y Val Arg Arg 470 475 <210> 338 <211> 477 <212> PRT <213> Corynebacter i um gl ut ai cur <400> 338 Met G u G n His y Thr Asn u 1 5 Ser Gy y Pro Ser G u Ala IVet Phe Asp Trp Val Leu Ala Pro Tyr 40 Ala Lys Val Leu His G n Ala Asp 55 Thr IVet Leu Ala y Leu Asp G n 70 Thr Phe y Pro Leu Pro Ser Asp Arg y Val lie Asp Arg Val y 100 at g NMbt 350 gaa G u at g Met gct Al a act Thr cgg Ar g 430 ggt Gy gcc Al a 335 act Thr ctt Leu gt g Val tgc Cys gat Asp 415 gaa Gu gga Gy gca Al a ggt Gy gca Al a cgc Ar g gt g Val 400 gaa G u gt g Val acc Thr agt Ser ttg Leu cca Pr o cag G n 385 cgg Ar g gaa G u ct c Leu gcg Al a acc Thr 465 gtt Val gca Al a 370 ggc Gy at c SIle ct c Leu acc Thr ggc Gy 450 gct Al a 340 acc Thr ttc Phe cca Pr o gag G u ggc Gy 420 gat Asp cgg Ar g gcg Al a 1123 1171 1219 1267 1315 1363 1411 1459 1507 1554 taagcattag tttatggcct gtg n Gy Phe 25 Asp Leu Leu G u Pr o 105 Ala Leu Trp 10 Ala Leu Ser Val Leu Al a Leu Ser Asp G y Lys Asp Asp Val Hi s 90 Su Val y Page 441 Gy Val Ser G u Val Gy Gy Gy Ser Lys Asp Al a Al a Ar g 110 Ar g Thr Al a Leu Asp bet Leu seql i st cor r ect ed. t xt Al a y Arg Ser Arg Asn Asp n Val Ala Thr Leu Phe Arg IVet Trp 115 120 125 Val Arg Asp Ala Val Arg Asp lie Ala Leu y Thr Thr G u Leu Val 130 135 140 Asp Ala Leu Ser Ala G n Ala Lys Ala His Ala G y Ala I I e It Pro 145 150 155 160 G y Lys Thr His Phe G n Ala Ala G n Pro Val Leu Leu Ala His G n 165 170 175 Leu Leu Ala His Ala G n Pro Leu Leu Arg Asp Ile Asp Arg lie Arg 180 185 190 Asp Leu Asp Lys Arg Leu Ala Val Ser Pro Tyr y Ser y Ala Leu 195 200 205 Ala y Ser Ser Leu Lys Leu Asn Pro G u Ala lie Ala G u G u Leu 210 215 220 Sy Phe Asp Ser Ala Al a Asp Asn Ser lie Asp Ala Thr Ser Ser Arg 225 230 235 240 Asp Phe Ala Ser G u Thr Ala Phe Val Leu Ala G n Leu Ala Val Asp 245 250 255 IVet Ser Arg Leu Ala u Gu Ile Ile Ala Trp Cys Thr Pro u Phe 260 265 270 G y Tyr Ile Thr Leu Ser Asp Ser Trp Ser Thr G y Ser Ser Ile IVet 275 280 285 Pro G n Lys Lys Asn Pro Asp Val Ala G u Leu Thr Arg y Lys Ser 290 295 300 Sy Arg Leu Ile y Asn Leu Thr y Leu Leu Ala Thr Leu Lys Ala 305 310 315 320 G n Pro Leu Ala Tyr Asn Arg Asp Leu n G u Asp Lys G u Pro lie 325 330 335 Val Asp Ser Val Al a G n Leu Asn Leu Leu Leu Pro Al a IVet Thr G y 340 345 350 Leu Val Ser Thr Leu Thr Phe Asn Thr G u Arg Iet Arg G u Leu Ala 355 360 365 Pro Ala y Phe Thr Leu Ala Thr Asp Leu Ala G u Trp IVet Val Arg 370 375 380 G n y Val Pro Phe Arg G u Ala Hi s G u Ala Ser G y Ala Cys Val 385 390 395 400 Arg lie Ala u Ser Arg y Val Asp Leu Ile Asp Leu Thr Asp G u 405 410 415 G u Leu Ser y Val Asp Ala Arg Leu Thr Pro G u Val Arg G u Val 420 425 430 Leu Thr Ile Asp y Al a Val Ala Ser Arg Ala Thr Arg y y Thr 435 440 445 Ala y Val Arg Val Al a G u G n Arg Ala Arg Val Asp Ala Ala Ser 450 455 460 Thr Ala His Ala G u Trp Ala Arg Ala G y Val Arg Arg 465 470 475 Page 442 seql i st cor r ect ed. t xt <210> 339 <211> 906 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(883) <223> FRXA02161 <220> <221> m sc feature <222> 863 <223> n a, c, g, or t <220> <221> VARIANT <222> 255 <223> Xaa Cys, Arg, Ser, or y <400> 339 gatcgctaac aagcgcgatc gcgaagctgg caacaactaa gactagaact tcaagtattt agaaagtaga agaacaccac gccacctttt caagcatcca gaa cag cac G u G n Hi s acc Thr gag G u gcc Al a caa G n ctt Leu cct Pr o cgc Ar g aac Asn cgc Ar g caa G n 150 gaa G u at g let t at Tyr gat Asp cag G n gat Asp ggt Sy cag G n 120 at c SIle aag Lys gcg Al a gcc Al a gt g Val ctt Leu ggc G y gat Asp gag G u gca Al a ct g Leu cat Hi s ct g Leu tt g Leu tt g Leu tct Ser aag Lys 75 gt g Val gt g Val acc Thr gga G y gca Al a 155 tgg Tr p agt Ser gcc Al a gat Asp gat Asp cac Hi s ggc G y ct g Leu aca Thr 140 ggc Gy ggc Gy gt c Val t cc Ser 45 gaa G u gt c Val ggc G y G y ttc Phe 125 acc Thr gcg Al a ggc Gy t cc Ser aag Lys gat Asp gcc Al a gcg Al a cgt Ar g 110 cgc Ar g gag G u at c l Ie cgc Ar g 15 act Thr gca Al a ct a Leu gac Asp at g Met 95 ct g Leu at g let ctt Leu at g M\et ttc Phe cat Hi s cac Hi s gcc Al a gga G y 80 gaa G u cgc Ar g tgg Tr p gt c Val cca Pr o 160 ggt Gy gac Asp aag Lys at g Met ttc Phe ggt G y ggt G y cgc Ar g 130 gcc Al a aag Lys gga Gy tgg Tr p gtt Val ct g Leu ggt G y gt g Val cgt Ar g 115 gac Asp ct c Leu acc Thr ccc Pr o gtt Val ttg Leu gct Al a ccg Pr o at t SIle 100 t cc Ser gca Al a agc Ser cac Hi s gga Gy t cc Ser ttg Leu cac Hi s ggg Gy ctg Leu gac Asp cgc Ar g gtg Val gcc Al a ttc Phe 165 Page 443 cag gca gct cag ccg gtc c t O31n Al a Al a O31n Pr o Val L 1 70 cag cct t tg ct g cgc gat at O31n Pr o Leu Leu Ar g Asp 1I1 1 85 ct t gcg gt g t ct cct tac g~ Leu Al a Val Ser Pr o Tyr 03 200 aag ctc aac cct gaa gca at Lys Leu Asn Pro O31u Al a 11 2152 gca gat aac t cc at t gat g Al a Asp Asn Ser Ilie Asp Al 230 235 acc gcc t tc gt g ct g gcg c~ Thr Al a Phe Val Leu Al a 03 250 t gaagaaat c at cgcat ggt gca <210> 340 <211> 261 <212> PFTF <213> Cor ynebact er i urn gI ut <220> <221> VARIANT eu jt y :c e a n seqI i st car r ect ed. t xt ct g gca cac cag ct g ct g Leu Al a Hi s 03 n Leu Leu 1 75 gat cgt at c cgt gac ctg9 Asp Arg Ilie Arg Asp Leu 1 90 t cc ggc gca ct t gct ggt Ser 03 y Al a Leu Al a 03 y 205 210 gct gaa gaa ct c ggc tt t Al a 03 u 03 u Leu 03 y Phe 225 acc agc tcc cgc gat ttc Thr Ser Ser Ar g Asp Phe 240 ct t gca ngt gga t at gt c Leu Al a Xaa 03 y Tyr Val 255 gca Al a g ac As p 1 t cc Ser g at As p gca Al a ccg Pr o c ac Hi s 1 aag Ly s t ct Ser t cc Ser t ct Ser ct t Le u 260 gca Al a cgt Ar g tt g Le u gca Al a g aa O31u 245 ggc (31y ani cumn <222> 255 <223> Xaa Cys, Ar g, Ser or G31y <400> 340 [Vb t G31u (1n Ser 03 y 03 y Phe As p Tr p Al a Lys Val Thr I'b t Leu Thr Phe G31y Ar g G31y Val Ala G31y Ar g 115 Val Ar g As p 130 Asp Al a Leu 145 Hi s 03 y Pr o Ser Val Leu Leu Hi s Al a 03 y Pro Leu 1 1 e As p 1 00 Ser Ar g Al a Val Ser Al a Th r As n 03 u 03 y Al a L eu Tr p 03 y 03 y Al a Pr o Al a 55 As p Ser ValI As p As p 135 Al a Ph e 25 As p Le u Le u O31u Pr o 1 05 ValI Al a Al a 10 Al a Leu Ser Val Leu Al a Leu Ser Asp 03 y Lys Asp Asp Val Hi s 90 31u Val G31y Al a Thr Leu Leu 03 y Thr 140 Hi s Al a (3 y 1 Page 444 ValI Ser O31u ValI o31y o31y Ph e 125 Thr Al a Ser Lys5 As p Al a Al a Ar g 110 Ar g O31u I Ie Ar g Thr Al a Le u As p Le u Le u Sy Leu Asp Al a Sy 225 Asp Tyr Thr Al a Asp 195 Ser Asp Al a Pr o Phe 165 Al a Ar g Leu Al a G u 245 Gy G n G n Leu Lys Al a 230 Thr Al a Pr o Al a Leu 215 Asp Al a seql i st cor r ect ed. t xt Ala G n Pro Val Leu Leu 170 Leu Leu Arg Asp Ile Asp 185 Val Ser Pro Tyr y Ser 200 205 Asn Pro u Ala lie Ala 220 Asn Ser Ile Asp Ala Thr 235 Phe Val Leu Ala G n Leu 250 Al a Ar g 190 Gy G u Ser Al a Hi s 175 I e Al a G u Ser Xaa 255 <210> 341 <211> 786 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(763) <223> FRXA02162 <220> <221> m sc feature <222> 94 <223> n a, c, g, or t <400> 341 ctcggctttg attccgcagc agataactcc attgatgcca tctgaaaccg cct t cgtgct ggcgcagctt gcangtggat gaa G u tct Ser cct Pr o aac Asn aac Asn cag G n at c SIle t cc Ser gt g Val acc Thr gac Asp aac Asn gca Al a t cc Ser gag G u ct g Leu cag G n ct g Leu tgg Tr p aca Thr ct g Leu ct g Leu gaa G u 75 ct c Leu tgc Cys ggc G y acc Thr gct Al a gat Asp cct Pr o acc Thr agc Ser cgt Ar g 45 acc Thr aag Lys gca Al a cca Pr o t ca Ser ggc G y ct g Leu gaa G u at g M\et gaa ttt G u Phe 15 at c at g I e bet aag tct Lys Ser aag gca Lys Al a cca at c Pro Ile 80 act ggt Thr G y 95 Page 445 ccagctcccg cgatttcgca atg tcc cgc ttg gct IVet Ser Arg Leu Ala 1 ggt tac atc acc ttg Gy Tyr Ile Thr Leu ccg cag aag aag aac Pro G n Lys Lys Asn ggt cgc ttg atc ggt Gy Arg Leu Ile y cag cct tta gcg tac G n Pro Leu Ala Tyr gta gat tcc gtg gcg Val Asp Ser Val Ala ttg gtt tcc acc ttg Leu Val Ser Thr Leu 100 acc ttc aac acc gag cgc atg Thr Phe Asn Thr G u Arg Mbet 105 ctt gcc acc gac ttg gct gag Leu Ala Thr Asp Leu Al a G u 120 cgt gag gca cac gaa gca tcc Arg G u Ala His u Al a Ser 135 140 agg gga gtg gac ctt atc gat Arg Gy Val Asp Leu Ile Asp 150 155 gat gca cgt ctg acc cca gag Asp Ala Arg Leu Thr Pro G u 170 gca gtg gct tcc cgt gca acg Ala Val Ala Ser Arg Ala Thr 185 gcg gag caa cgc gca cgt gtc Ala G u G n Arg Ala Arg Val 200 tgg gca cgt gcg ggg gta cgt Trp Ala Arg Ala y Val Arg 215 220 <210> 342 <211> 221 <212> PRT <213> Cor ynebact er i um gl ut ar <400> 342 IVet Ser Arg Leu Ala G u G u 1 5 Sy Tyr lie Thr Leu Ser Asp Pro G n Lys Lys Asn Pro Asp Sy Arg Leu Ile y Asn Leu G n Pro Leu Ala Tyr Asn Arg 70 Val Asp Ser Val Ala G n Leu Leu Val Ser Thr Leu Thr Phe 100 Pro Ala y Phe Thr Leu Ala 115 G n y Val Pro Phe Arg G u 130 135 Arg lie Ala u Ser Arg y seql i st cor r ect ed. t xt cgt gaa ctt gca cca gca ggt ttc Arg G u Leu Ala Pro Ala y Phe 110 115 tgg atg gtg cgc cag ggc gtt cca Trp bet Val Arg G n Gy Val Pro 125 130 ggc gct tgc gtg cgg atc gcg gag Sy Ala Cys Val Arg Ile Ala u 145 ct c act gat gaa gaa ct c agt ggc Leu Thr Asp G u G u Leu Ser G y 160 gta cgg gaa gtg ctc acc att gat Val Arg G u Val Leu Thr Ile Asp 175 180 cgc ggt gga acc gcg ggc gtg cgg Arg G y y Thr Ala y Val Arg 190 195 gat gcc gca agt acc gct cac gcg Asp Ala Ala Ser Thr Ala His Ala 205 210 cga taagcattag tttatggcct gtg Ar g lie lie Ala Trp Cys Thr Pro u Phe Tr p 25 Al a Sy Leu Leu Thr 105 Asp Hi s Asp 10 Ser Thr G u Leu Leu Leu Gn Gu 75 Leu Leu I 90 G u Arg I Leu Al a G u Al a Leu Ile Page 446 Ser Ar g Thr Lys Al a Ar g Tr p 125 Sy Leu Ser Sy Leu G u Mbet G u 110 Met Al a Thr I e Lys Lys Pr o Thr Leu Val Cys Asp Met Ser Al a SIe Sy Al a Ar g Val G u seqi i st car r ect ed. t xt 145 150 155 160 O31u Leu Ser G31y ValI As p Ala Ar g Leu Thr Pro O31u Val Ar g O31u Val 165 170 175 Leu Thr I Ie As p G31y Ala Val Ala Ser Ar g Ala Thr Ar g 31y G31y Thr 180 185 190 Ala O31y Val Ar g Val Ala O31u O31n Ar g Ala Ar g Val As p Ala Ala Ser 195 200 205 Thr Ala Hi s Ala O31u Tr p Ala Ar g Ala G31y Val Ar g Ar g 210 215 220 <210O> 343 <21 1> 1269 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221 CDS <222> (1246) <223> RXA02262 <400> 343 gcaccaattt cggacctgaa atccccgagg aaaccgtgcc cgacgccgtg caggtgggcg t cgat aagca aaaaat cgct gat act cgaa aggcct caaa at g acc gca acc t ac l'bt Thr A a Thr Tyr ac c Thr at c I Ie g aa (31u aac As n tt t Ph e ccc Pr a gga G31y ac c Thr t cc Ser g aa (31u gcc A a ct c Le u aac As n ggc G31y t ac Tyr aac As n gt c ValI 120 aac As n gcc A a gt c ValI ct c Le u cat Hi s ccc Pr a G31y g aa (31u aac As n ct g Le u at c SI e aaa Lys5 t t g Le u ggc G31y aag Lys5 75 ggg G31y aac As n gac As p t cc Ser aat As n g ac As p gcg A a gcg A a g ac As p cga Ar g aag Lys5 ac g Thr gcc A a 140 tt c Ph e gt c ValI c ag O31n 45 at g G31y tt c Ph e gcc A a gt c ValI 125 t ac Tyr tt g Le u gcg A a ggc G31y at c I Ie ccc Pr a aaa Lys5 t ca Ser 110 ac c Thr cgc Ar g tt c Ph e 15 c aa O31n g ac As p ac c Thr g ac As p aac As n 95 ggc G31y ggt G31y ac c Thr tt g Le u t gc cys t ac Tyr tt c Ph e cgc Ar g ac c Thr tt g Le u gca A a ggc G31y g aa (31u g at As p at g vb t g aa (31u tt c Ph e gt g ValI cgc Ar g aag Lys5 130 gt t ValI ccg Pr a gt c ValI gcc A a aac As n at g vb t aag Ly s t cg Ser 115 gcc A a ccc Pr a g ac As p at g vb t ggt G31y cca Pr a gcg A a t gg Tr p 1 00 at c I Ie at c G31y Page 447 ggc G31y 150 gga G31y cgc Ar g at c l Ie gt g Val gt c Val 230 t ac Tyr gcc Al a gt t Val cca Pr o ct g Leu 310 gat Asp at c l Ie acC Thr gtg aag cac tta gcg gtc gc Val Lys His Leu Ala Val Al 155 seqi i st car r ect ed. t xt c gac gcg aca acc ttg a Asp Ala Thr Thr Leu gct gtc gtc Al a Val Val ggt G31y gga G31y gcc Al a 200 gcc Al a gcc Al a aaa Lys ggt G31y gac Asp 280 gcc Al a ct g Leu t gc Cys t at Tyr gt g Val 360 at g N/bt 170 acc Thr gca Al a gga G31y acc Thr gaa (31u 250 ttc Phe at g lvb t caa O31n gga G31y ggc G31y 330 gt c Val gaa (31u gcg Al a acc Thr aca Thr t ct Ser acc Thr 235 t gg Tr p gac Asp ggg G31y ct a Leu aaa Lys 315 aag Lys ggc G31y aac Asn aaa Lys at c l Ie t gg Tr p gaa G31u 220 acg Thr ct C Leu gac Asp ct C Leu ct C Leu 300 ct C Leu ct a Leu ggc G31y gcc Al a acc Thr aag Lys gcg Al a 205 gaa G31u gac Asp aag Lys gct Al a t ac Tyr 285 ggc G31y gac Asp ccc Pr o at g lvb t ct g Leu 365 at c l Ie at c l Ie 190 ttg Leu gac Asp gcc Al a ccg Pr o t at Tyr 270 gaa G31u at t l Ie ct t Leu gga G31y cca Pr o 350 gaa (31u acc Thr 175 aag Lys gaa G31u gt g Val gcc Al a ggc (31y 255 ttg Leu gcc Al a cca Pr a gca Al a cgc Ar g 335 gt a Val aaa Lys 160 gaa G31u gga G31y aaa Lys gt c Val ggc G31y 240 gca Al a ct t Leu t gg Tr p gga G31y aag Lys 320 acc Thr gaa gu (31y gcg Al a cgc Ar g ttc Phe aaa Lys 225 t cc Ser ttg Leu gac Asp gca Al a acc Thr 305 at t l Ie aac Asn gac Asp gt c Val t gc Cys agc Ser ccc Pr a 210 gac Asp t cc Ser ct g Leu gac Asp gaa G31u 290 cac Hi s t cc Ser gat Asp gt c Val ggc 31y 370 at c l Ie gaa G31u 195 gag G31u gcc Al a gcc Al a ct g Leu gcc Al a 275 gaa O31u t gg Tr p cag O31n gag O31u gcc Al a 355 acc Thr gca Al a 180 cgc Ar g at c l Ie gac Asp ttc Phe ct t Leu 260 cgc Ar g t ac Tyr t ac Tyr at t l Ie gaa O31u 340 t gg Tr p aca Thr 165 gag G31u ggc G31y gaa O31u at c l Ie cca Pr a 245 cca Pr a ct C Leu ggc G31y gac Asp ggc G31y 325 at c l Ie gca Al a ttg Leu 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1266 1269 aac ctg tgg gaa tca ccc gca ctg gct Asn Leu Trp 3u Ser Pro Ala Leu Ala 375 380 aaa <210> 344 <211> 382 <212> PRT <213> Cor ynebact er i ur gl ut ai cum tgagagaaga aacaacaatg Page 448 seqi i st car r ect ed. t xt <400> 344 l'bt Thr A a Thr Tyr Thr Thr 03 u Thr A a I I e Asn Phe Leu Phe Leu Ser ValI Ly s Pr o Ar g G31y Pr o Pr o A a 145 Th r A a Ar g Ph e Ly s 225 Ser Le u As p A a Th r 305 li1e As n Pr o ValI A a As n vb t Ly s Ser 115 A a Pr a A a I Ie (31u 1 95 (31u A a A a Le u A a 275 (31u Tr p O31n O31u vb t O31u Le u O31u vb t Ty r Hi s b t ValI ValI 1 65 O31u G31y Ou le Pr a 245 Pr a Le u G31y As p G31y 325 li1e A a Th r Ser O31u A a Ser Ph e A a 135 ValI Pr a Pr a As n ValI 215 li1e Ph e A a ValI O31n 295 A a I Ie Le u A a Le u 40 As n G31y Ty r As n ValI 120 As n Ly s G31y G31y A a 200 A a A a Ly s G31y As p 280 A a Le u cys Ty r G31y ValI Ser vb t Le u A a 1 05 Le u Le u Hi s ValI I Ie 1 85 Ph e ValI A a Ly s Ar g 265 Ty r Ty r O31n Ser Ser 345 Ly s Le u G31y Ly s G31y As n As p Ser A a 1 A a Th r Th r Ser Th r 235 Tr p As p G31y Le u Ly s 315 Ly s G31y As p A a A a As p Ar g Ly s Th r A a 140 ValI Ly s I Ie Tr p O31u 220 Th r Le u As p Le u Le u 300 Le u Le u G31y ValI O31n vb t G31y Ph e A a ValI 125 Ty r A a Th r Ly s A a 205 O31u As p Ly s A a Ty r 285 G31y As p Pr a vb t A a Gy le Pr a Ly s Ser 110 Th r Ar g As p I Ie I Ie 1 Le u As p A a Pr a Ty r 270 Ou le Le u G31y Pr a 350 O31n As p Th r As p As n G31y G31y Th r A a Th r 1 Ly s O31u ValI A a G31y 255 Le u A a Pr a A a Ar g 335 ValI Asp Val Ala Trp Ala Thr O31n Val Tyr O31u Asn Ala Leu O31u Lys G31y Page 449 seqi i st car r ect ed. t xt 355 360 365 Val G31y Thr Thr Leu Asn Leu Trp O31u Ser Pro Ala Leu Ala 370 375 380 <210> 345 <211> 1065 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1042) <223> RXA00219 <400> 345 tttgccgtac atgcgcgagc acctcctcaa cagcccgcac caccgaccaa tcacataaga cacaagcact aaaacagcat t aaagaaaga aagct tt t tc aac As n ggc G31y t ac Tyr cat Hi s t gg Tr p t cc Ser ag g Ar g tt g Le u cgc Ar g g aa O31u 1 50 gcc A a t cc Ser t at Tyr ac c Thr gt c ValI gct A a ct g Le u tt c Ph e gca A a 120 cca Pr o tt c Ph e gct A a c aa O31n ggt G31y G31y G31y ggc G31y at t lIe g at As p ct t Le u gt a ValI ccc Pr o ac g Thr 1 70 t cc Ser gaa O31u t gg Tr p caa O31n gct A a 75 act Thr gt t ValI gcc A a aag Ly s gca A a 155 ccg Pr o cgc Ar g t at Tyr tt g Le u ccg Pr o cgg Ar g c ac Hi s t ac Tyr cgc Ar g at t I Ie 140 ag c Ser c ag O31n c aa O31n gt c ValI ag c Ser gca A a tt c Ph e ggc O31y c ag O31n 110 t cg Ser gt g ValI g at As p tt c Ph e gct A a 15 at c I Ie at t I Ie ct g Le u at c O31y 95 t ca Ser cgt Ar g g at As p gt g ValI ac c Thr 1 75 gcc A a gag O31u aat As n g aa O31u g at As p 80 ggt O31y cgc Ar g g aa O31u g at As p at c I Ie 1 60 ac c Thr gt g ValI 1 aac As n tt g Le u ggc O31y tt t Ph e gcg A a gcg A a aac As n t gg Tr p gcc A a 145 at c I Ie gt g ValI ac g Thr gag O31u gt g ValI gag O31u cat Hi s t gc cys act Thr tt c Ph e 130 cga Ar g cgt Ar g gag O31u gcc cgt aag Ala Ar g Lys at t lIe g at As p ag c Ser at g vb t g at As p at g vb t 1 00 gt g ValI at t I Ie gt g ValI gt t ValI tt t Ph e 1 aaa Ly s gct A a t cc Ser t ct Ser cgg Ar g gca A a gcc A a g aa O31u ccg Pr a gca A a tt t Ph e 1 gag O31u cac t gt cac cgt ggc ct t gct ccc ggc gga t tg t ac gt t gcc aac t gt Page 450 His Cys His Arg G y 185 Leu Al a Pr seql i st cor r ect ed. t xt o G y G y Leu Tyr Val 190 Al a Asn Cys 195 ggc gat cat tcg gat ctg cgc gga gct Gy Asp His Ser Asp Leu Arg Gy Ala 200 205 atg gag gtg ttc gag cac gtc gcg gtc Met G u Val Phe G u His Val Ala Val 215 220 aaa ggg cgc cgt tac ggc aac atc att Lys Gy Arg Arg Tyr Gy Asn lie lie 230 235 ttc ttt agc tcc aac agc acg gaa gcg Phe Phe Ser Ser Asn Ser Thr G u Ala 250 ctt ggc ggc ggc gtt cca gcg cag tac Leu G y G y G y Val Pro Ala G n Tyr 265 270 aaa ttc gcc tcg gga gcc cag gcc cgc Lys Phe Ala Ser y Al a n Ala Arg 280 285 caa atg ccg agt gat act cca caa cac G n Met Pro Ser Asp Thr Pro G n His 295 300 tca aac aca cag cca taaaaaattc cgct Ser Asn Thr G n Pro 310 <210> 346 <211> 314 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 346 Val Ala Arg Lys Lys Asn Thr Ser Asp 1 5 Asn Thr Pro lie Ala y Thr Tyr u Leu G u Ala Asp Ser Tyr Thr Thr Asp 40 Sy Val Pro Ser Ser His lie Val Leu Phe G u Tyr IVet Arg Trp Ile Ala Thr 70 Ala His G n Asp Ala Ser Lys Leu Arg Ala Cys Thr IVet Ala Arg Tyr Phe Ala 100 105 Asn Thr Val Val G u Leu Asp Ala G u 115 120 Trp Phe Asp lie Pro Arg Ala Pro Arg 130 135 aaa Lys at c SIle ttg Leu t cc Ser 255 aag Lys cac Hi s cct Pr o tct Ser gcc Al a at g Met 240 gcg Al a gat Asp gat Asp gcg Al a gag G u gat Asp 225 ggt G y at t l Ie gaa G u ggg G y gaa G u 305 ct c Leu 210 ccc Pr o t ca Ser acc Thr t cc Ser gt c Val 290 acg Thr gcg Al a ccg Pr o gac Asp cgt Ar g tgg Tr p 275 tct Ser ccg Pr o gga G y at g Met acc Thr gag G u 260 gt g Val acc Thr gag G u 739 787 835 883 931 979 1027 1065 ggcgcg tcc G n Ser Al 10 G y G u T, G y Trp Li G y G n Pi G y Al a Al Ile Thr Hi 90 Asp Val T. Leu Al a Al Val Lys II 1 Page 451 Ser Ser I e G n Al a Leu Pr o Leu 125 Ar g Al a I e I e Leu SIe Sy Ser Ar g Asp Al a 145 I le Val Tyr G u Asp 225 Gy I e Su Gy Su 305 let Asp Phe Al a 195 Al a Pr o Asp Ar g Tr p 275 Ser Pr o Al a Phe 165 G u Cys let Leu G u 245 Leu Ar g Leu Hi s G u 150 Al a Hi s Gy bet Lys 230 Phe Leu Lys G n Ser 310 Ser Sy Cys Asp G u 215 Sy Phe Sy Phe bet 295 Asn seql i st cor r ect ed. t xt Phe Thr Pro Ala Ser Arg 155 Ala lie Thr Pro n Asn 170 His Arg G y Leu Ala Pro 185 His Ser Asp Leu Arg G y 200 205 Val Phe G u His Val Ala 220 Arg Arg Tyr Gy Asn Ile 235 Ser Ser Asn Ser Thr G u 250 G y G y Val Pro Ala G n 265 Al a Ser G y Al a n Al a 280 285 Pro Ser Asp Thr Pro G n 300 Thr G n Pro Asp Phe Sy 190 Al a Val SIle Al a Tyr 270 Ar g Hi s Val Thr 175 Sy Lys SIle Leu Ser 255 Lys Hi s Pr o SIe 160 Thr Leu Ser Al a let 240 Al a Asp Asp Al a <210> 347 <211> 1662 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1639) <223> RXA01508 <400> 347 ttccgggacg tttccgtgat ctcgtggaag atcccaaact ccgccgtcat cttgatcgtg gtgggaaccg taaacgctgc ccc Pr o tcg Ser aac Asn gct Al a tgg Tr p ct g Leu ggc Sy ctt Leu tgg Tr p t at Tyr at t SIle ctt Leu ct g Leu gag G u gt a Val ggt G y tta Leu ct a Leu gaa G u gca Al a tta Leu gcc Al a acc Thr 45 ct g Leu gtt Val ct g Leu t cc Ser ct g Leu t cc gt c Ser Val 15 gta t cg Val Ser ct c at c Leu II e gtc aag Val Lys Page 452 ccgttccggt gccgtcgtgg atg tct gat tta gga IVet Ser Asp Leu y 1 tcc att tgt gcg gca Ser lie Cys Ala Ala ctt tcc acc agc ttg Leu Ser Thr Ser Leu gtc gca ggt tat gta Val Ala y Tyr Val ccg ttt ctc aac tgg Pro Phe Leu Asn Trp s cct gcg caa acc ttc ct c ggt gtg Pro Ala G n Thr Phe Leu y Val eql i st cor r ect ed. t xt gaa acc ctc ctt gg G u Thr Leu Leu G a ctt att y Leu I e ggt Gy t cc Ser ggc Gy gcc Al a gat Asp 150 ct g Leu aac Asn ctt Leu at c SIle acc Thr 230 tct Ser ct c Leu t at Tyr gt g Val cga Ar g 310 t cc Ser tgg Tr p gaa G u 120 gcc Al a ctt Leu tgg Tr p gtt Val cgc Ar g 200 gcg Al a cgc Ar g t ac Tyr ct c Leu gaa G u 280 at c SIle cca Pr o ct g Leu ct g Leu cca Pr o acc Thr gca Al a ggc Gy 170 gca Al a cac Hi s gcc Al a cag G n gac Asp 250 ggc Gy ct g Leu ggc Gy at g let ct g Leu at t SIle ct g Leu gga Gy 140 tta Leu at g Met ttc Phe ttc Phe gt g Val 220 t ac Tyr gt a Val ttg Leu t at Tyr ggc Gy 300 at c SIle t at Ty r gcc Al a at g let 125 tct Ser ggt Gy cgc Ar g gt g Val gt a Val 205 ttg Leu cgc Ar g gt g Val cag G n cca Pr o 285 gat Asp acc Thr ttc Phe acc Thr 110 acc Thr ct g Leu ggc Gy ggt Gy ggc Gy 190 t cc Ser gt g Val gac Asp aca Thr t at Tyr 270 agc Ser ggc Gy caa G n acc Thr 95 gct Al a at g let gtt Val ct g Leu gcc Al a 175 tgt Cys gt g Val aaa Lys ccc Pr o gaa G u 255 t cc Ser ctt Leu ct c Leu gtt Val tct Ser 335 ttc Phe gca Al a at c SIle gcc Al a gcc Al a 160 gca Al a gt g Val gt g Val t cc Ser gt g Val 240 cga Ar g act Thr aat Asn gca Al a gaa G u 320 gac Asp gcg Al a at t l Ie cag G n acc Thr 145 tgg Tr p gca Al a ct g Leu gcg Al a gac Asp 225 at c SIle ggc Sy cgt Ar g cca Pr o gca Al a 305 tta Leu aat Asn acc Thr ggc Gy caa G n 130 ttg Leu cct Pr o gcc Al a ct g Leu ctt Leu 210 ggg Sy t at Tyr aaa Lys gac Asp gag G u 290 cgg Ar g gac Asp ggg G y at c SIle at c SIle 115 ggc G y aat Asn ttt Phe gga Gy cga Ar g 195 ctt Leu at c SIle t ca Ser gac Asp cag G n 275 gca Al a gaa G u cca Pr o gga G y ggc Gy 100 ct g Leu cgc Ar g gct Al a gt g Val at g Met 180 cat Hi s ct c Leu gtt Val cac Hi s cga Ar g 260 cat Hi s gaa G u ct c Leu gaa G u gcg Al a 340 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 atc gaa gta gcc aac aca gtg ctg cgc Ile u Val Ala Asn Thr Val Leu Arg 330 Page 453 gaa gat ccc cgc gt c Ou Asp Pro Arg Val 345 seqI i st car r ect ed.I t cc at c at c gt t gac gac gct Ser Ilie lieVal Asp Asp Ala t tc acc t gg ct g Phe Thr Tr p Leu cgc Ar g ct t Le u t tc Ph e 390 gt g ValI gga G31y gt g ValI gcc A a ct t Le u 470 c ag O31n gag O31u ggc G31y 360 g ac As p ac c Thr t cc Ser ac c Thr gt t ValI 440 at g vb t g at As p cgt Ar g ct g Le u aat As n aac As n gcc A a ag c Ser 410 aaa Ly s ac a Thr tt a Le u gt t ValI tt g Le u 490 aat As n aac As n cga Ar g 395 gcc A a t cg Ser tt t Ph e gag O31u ct g Le u 475 gaa O31u ggc G31y g ac As p 380 gca A a t ac Tyr gcg A a ggc (31y ct t Le u 460 gt g ValI cct Pr o g aa G31u 365 ac c Thr cga Ar g ac c Thr ggc G31y g ac As p 445 cgg Ar g gct A a t cc Ser 350 act Thr at g vb t ct g Le u act Thr t gt cys 430 t gg Tr p g aa (31u gct A a ac g Thr g aa (31u 510 t ac Tyr gcc A a aac As n cca Pr o 415 g aa (31u G y g ac As p ggg G31y ct g Le u 495 g at As p ag g Ar g g aa O31u 400 g at As p c aa O31n tt c Ph e ac c Thr gt g ValI 480 g at As p t cc Ser ct g Le u 385 c aa O31n gt g ValI gt c ValI c aa G n ccg Pr o 465 tt t Ph e cat Hi s at c I Ie 370 t at Tyr G31y tt c Ph e at c I Ie ct g Le u 450 cca Pr o ggg G31y ccc Pr o 355 at c I Ie t ca Ser cgc Ar g t gg Tr p cca Pr a 435 t gt cys ct g Le u tt g Le u cgc Ar g at c I Ie g aa (31u at g vb t cga Ar g 420 t at Tyr G y act Thr g at As p gt g ValI 500 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 1 603 1 649 1 662 aag gga tac cgc Lys Gy Tyr Arg t ca ggc gac Se r 03 y As p t agct gcaac gat gcgct gt gt g <210O> 348 <211> 513 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 348 I'bt Ser Asp Leu Gy Pro i~e Trp Arg 1 5 Ser Ilie Cys Ala Al a Ser G31y Leu Val 25 Leu Ser Thr Ser L eu As n G31y 31y G31y 40 Val Ala G31y Ty r Val Ala Ala Leu Gy 55 Pr o Phe Leu As n Tr p Pro Ala O31n Th r 70 Tr p Leu Leu Leu Val Ser Val 10 Tyr (31u Leu Ala Leu Val Ser li1e Val O31u Th r Ser Leu I Ie Leu G31y Ala Leu Leu Val Lys Phe Leu G31y Va 1O31u Thr Leu 75 Page 454 Leu G y Leu Ile Al a SIle G n Thr 145 Tr p Al a Leu Al a Asp 225 I le Sy Ar g Pr o Al a 305 Leu Asn Al a Ser Leu 385 G n Val Val l e I e 115 Sy Asn Phe Sy Ar g 195 Leu SIle Ser Asp G n 275 Al a G u Pr o Sy Thr 355 I le Ser Ar g Tr p Pr o 435 Sy G n Val Leu Al a Leu 165 I le Leu Al a Al a G n 245 Ar g Ar g Ser Leu Val 325 vbt Leu Asp G u Val 405 Val Hi s seql i st cor r ect ed. t xt G y Cys Ser Ala Leu Val Leu Tyr 90 Leu Al a Asp 135 Tyr Pr o Leu Pr o Al a 215 Al a Asp Tyr Thr Leu 295 Phe G u Asp Ser Pr o 375 Tyr G n Al a Hi s Tr p G u 120 Al a Leu Tr p Val Ar g 200 Al a Ar g Tyr Leu G u 280 I le Pr o Val Pr o Sy 360 Asp Thr Ser Thr Val 440 I e Leu 105 Leu Pro Lys Thr G y Al a Leu G y 170 Al a Al a 185 Thr His Leu Al a Al a G n Sn Asp 250 Asn y 265 Ser Leu I e G y Ser 'bet Al a Asn 330 Arg Val 345 Sy Asn Pro Asn Leu Al a Ser Ser 410 Leu Lys 425 Pro Thr Val Leu Thr Leu 155 bet Leu Phe Thr Leu 235 I le Sy Val Sy G n 315 Thr Ser Asn Asn Ar g 395 Al a Ser SIle Leu Sy 140 Leu b t Phe Phe Val 220 Tyr Val Leu Tyr Sy 300 I le Val SIle Sy Asp 380 Al a Tyr Al a Al a bet 125 Ser Sy Ar g Val Val 205 Leu Ar g Val G n Pr o 285 Asp Thr Leu SIle G u 365 Thr Ar g Thr Sy Phe Thr Phe Thr Al a Al a 110 Thr Met Ile Leu Val Al a G y Leu Al a 160 G y Al a Al a 175 G y Cys Val 190 Ser Val Val Val Lys Ser Asp Pro Val 240 Thr G u Arg 255 Tyr Ser Thr 270 Ser Leu Asn G y Leu Al a Sn Val u 320 Arg Ser Asp 335 Val Asp Asp 350 Thr Tyr Asp IVet Al a Arg Leu Asn G u 400 Thr Pro Asp 415 Cys G u n 430 Trp G y Phe Phe G y Asp 445 Page 455 seqi i st car r ect ed. t xt O31n L eu Cy s O31y Pr o Al a As p 'b t O31u L eu O31u L eu Ar g O31u As pTh r 450 455 460 Pr o Pr o Leu Thr Phe Leu Asn As p O31u Val Leu Val Ala Ala O31y Val 465 470 475 480 Phe O31y Leu Asp Asn O31n Pr o Ar g O31u Leu O31u Pr o Ser Thr Leu Asp 485 490 495 Hi s Pr o Ar g Val Val O31u Asp Leu Arg Lys O31y Tyr Ar g O31u Ser O31y 500 505 510 Asp <210O> 349 <21 1> 924 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (901) <223> RXA01757 <400> 349 cgct tat tga acggat gcct ct cgat caag ccaacgaggc gt aaaccacg t t tccgt at t gt ct tggagc cgaat tcat a t at tgcacgt at t tcagct g cca aca gca Pro Th r Al a cca Pr o gcc Al a gcc Al a tt g Le u gt g ValI at c I Ie ct g Le u gcc Al a tt a Le u t at Ty r c aa O31n t ca Ser ct g Le u c aa O31n ggt O31y ggt O31y t cg Ser 120 ct C Le u gt c ValI tt g Le u gt t ValI ct c Le u gt c ValI gcg Al a ccc Pr o gcg Al a g ac As p gt t ValI gac As p ggc O31y ggc O31y aat As n 75 ct t Le u at a SI e ct c Le u ccc Pr o gt c ValI cgc Ar g ggc O31y gca Al a aat As n tt t Ph e g ac As p ggg O31y gt c ValI gt c gga Val O31y gca ggt Al a 03 y cga gca Ar g Al a 45 aca t gg Thr Tr p ct C ggc Leu 03 y gag acg O3 u Thr gct gct Al a Al a 110 ct t gca Leu Al a 125 cat t ct Hi s Ser gcc Al a 15 ct a Le u gt g ValI tt c Ph e ct c Le u ct t Le u 95 t ca Ser gcc Al a ggc O31y aac As n t ct Ser t gg Tr p ggc O31y gt c ValI ggc O31y c ag O31n t ct Ser aaa Lys5 c aa O31n aac As n tt c Ph e gcc Al a tt c Ph e aag Lys5 130 ggc O31y t gc Cys c ag O31n g aa O31u cct Pr a ccg Pr a c aa O31n 115 cca Pr a ct c Le u tt c Ph e t cc Ser cca Pr a c ag O31n ag c Ser 1 00 gca Al a gga O31y ct c agt gag gaa gat ggg gaa Leu Ser 03 u 03 u Asp 03 y 03 u Page 456 at c SIle 150 at t l Ie gat Asp gt g Val tcg Ser ggg Gy 230 cac Hi s gga Gy gt g Val gtt Val cca Pr o aat Asn 200 ggg Sy at t SIle gaa G u aat Asn tct Ser ccg Pr o 170 gac Asp gca Al a ttt Phe tgg Tr p gca Al a 250 aaa Lys t cc Ser 155 gca Al a gt g Val aag Lys ggg Sy gca Al a 235 gtt Val tct Ser seql i st cor r ect ed. t xt 140 145 aaa cag att gtg agg gca aag Lys n Ile Val Arg Ala Lys 160 ctc gct gcc gag ttg att ggt Leu Al a Al a G u Leu I e G y 175 cga aaa gca gcg cat cca caa Arg Lys Ala Ala His Pro G n 190 gag aaa tac acc tta ccc aca G u Lys Tyr Thr Leu Pro Thr 205 210 cat gag ctg ttc caa caa cca His G u Leu Phe n G n Pro 220 225 tca acg gaa gtt gcc act gag Ser Thr G u Val Ala Thr G u 240 cgt gca gga att cag gct gcg Arg Al a G y Ile n Al a Al a 255 taaacctcgt attttccctg ata cac Hi s ttc Phe cat Hi s 195 caa G n ct c Leu ttt Phe ctt Leu gt c Val acc Thr 180 at a SIle gcc Al a gga Sy ggt Sy caa G n 260 at c SIe 165 ct a Leu gct Al a gca Al a cat Hi s gga Sy 245 aca Thr <210> 350 <211> 267 <212> PRT <213> Corynebact <400> 350 bet Pro Thr Ala 1 I le Ser G y Leu Ile Lys Cys Phe Val n G n Ser Leu Asn G u Pro Thr Phe Pro G n Asp Ala Pro Ser 100 Arg Phe G n Ala 115 Leu Lys Pro y 130 er i um gl ut ari cum Ser Pro lie Tyr Asp 5 lie Al a Thr G n Leu 25 G u Al a Cys Ser Arg 40 Asp Leu Phe Leu Asp 55 Leu Val n G n Leu 70 Al a I e G u y Asp Arg Leu Arg y Asn 105 Sy Al a Ser Ser Leu 120 Val Leu G u Leu y 135 Val Val Val Leu Asp Arg Val y y Leu G y Al a Val Asn Asn Ala Leu Phe Pro lie Asp Al a Leu G y Asp Pro Val 140 Page 457 Val Al a Ar g Thr Leu G u Al a Leu 125 Hi s Sy Sy Al a Tr p Sy Thr Al a 110 Al a Ser G u 145 Al a SIle Pr o Pr o G n 225 Thr Al a Asp Hi s Phe Hi s 195 G n Leu Phe Leu Su I e 165 Leu Al a Al a Hi s Gy 245 Thr Val Al a Leu Met Al a 215 Ar g Leu Phe seql i st cor r ect ed. t xt Val Lys Ser Ser Lys G n 155 Val Pro Pro Ala Leu Ala 170 Pro Ala Asp Val Arg Lys 185 Asn Trp Ala Lys G u Lys 200 205 G y G y Phe G y Hi s G u 220 Ile His Trp Ala Ser Thr 235 G u Gy Ala Val Arg Ala 250 Asn Leu Lys Ser 265 SIle Al a Al a 190 Tyr Leu G u Gy Val G u 175 Al a Thr Phe Val SIe 255 <210> 351 <211> 636 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(613) <223> RXA02159 <400> 351 tgatggacca gcgtccaaag ttttcgatga agcagaaaac actgctggtg tggctgctgg ccaaccagcc gaggtaagac cgcctccacg ctcagaaagc atg tcc ctt ggc tca 11! let Ser Leu G y Ser acc Thr caa G n gt a Val gcc Al a ccc Pr o gcc Al a t ca Ser ct c Leu ct g Leu ttg Leu ggg Gy gaa G u ccg Pr o ttg Leu gaa G u cga Ar g cgc Ar g ct c Leu gaa G u cag G n tt g Leu gat Asp gcc Al a 75 cgg Ar g aac Asn at t SIle ct g Leu ct c Leu t ac Tyr ggt Sy tta Leu ttg Leu ct g Leu 45 gat Asp t ac Tyr ccg Pr o aat Asn gac Asp gat Asp gaa G u gcg Al a tcg Ser ccc Pr o 15 aaa Lys gaa G u ct c Leu gt c Val gag Su 95 gt g Val caa G n ggc Sy ggt Sy ggc Sy aag Lys 1 act Thr aaa Lys at c SIle gca Al a cca Pr o ct g Leu cgc Ar g gt c Val gat Asp cgc Ar g gt a Val cgc Ar g gca Al a agc Ser acc Thr gtt Val agc Ser at g let 100 gat gaa ctg ctg gtt tct aca gat cat tcc ggc Asp G u Leu Leu Val Ser Thr Asp His Ser G y aac atc gcg atg ctg Asn Ile Ala IVet Leu Page 458 cgc Ar g gt g Val ttc Phe 150 ct c Leu ccg Pr o 120 ct g Leu ct c Leu ggg Gy gga Gy gaa G u cgt Ar g acc Thr 170 gct Al a gt c Val gat Asp 155 act Thr seql i st cor r ect ed. t xt 110 115 gcc cag tac ctg gca agt ttc atc gat agg Ala G n Tyr Leu Ala Ser Phe Ile Asp Arg 125 130 gtt ggc acc atc gct ggt gat gac acc gtt Val y Thr Ile Ala y Asp Asp Thr Val 140 145 ccg ctc aca ggt aaa gaa cta ggt gaa tta Pro Leu Thr y Lys G u Leu Gy G u Leu 160 165 taaagcgccc ctagttcaag gct <210> 352 <211> 171 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 352 Mvt Ser L 1 Thr Arg T Lys Val T I e Asp I Al a Ar g L Pro Val A Leu Arg A Asn Ile A 1 Ser Phe I 130 Sy Asp A 145 G u Leu G eu G y hr Ala hr Ser le Thr ys Val sp Ser rg IVet 100 I a let 15 le Asp sp Thr y u Ser 5 Ar g G n G n Ar g SIle Leu Leu Ar g Val Leu 165 Thr Pro G n Al a Val G n Al a Thr 55 Pro Asp 70 Al a Ar g Asp u Arg Thr Val G y 135 Phe Val 150 Leu Ser Thr I e Ser Ser Sy Asp Leu 105 Pr o Lys Al a Ar g G u G n Leu Asp Al a Ar g Ser Al a Val Asp 155 Thr Asn SIle Leu Leu Tyr Sy Thr Al a Val 140 Pr o Leu Leu Leu Asp Tyr Pr o Asp G n 125 Sy Leu Pr o Lys G u Leu Val G u Ser Leu SIe Sy <210> 353 <211> 414 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(391) <223> RXN02154 Page 459 seqI i st car r ect ed. t xt <400> 353 cccagaacct cggcgaagt c agcgacaagc cagt caaggt gagct tcacc ccagt gct tg caccgttacc tcgcgaattc tcaccactgc aaccgcacct t tg aaa gaa ggc gt t 115 Leu Lys 03 u 03 y Val 1 acc gca gaa cag gct cgc gca gta tat gaa gag ttc tat gca cag gaa 163 Thr Al a 31u O31n Al a Ar g Al a ValI Tyr O31u O31u Phe Tyr Al a O31n 31u 15 acc ttc gtg cat gtt ctt cca gaa ggt gca cag cca caa acc caa gca 211 Thr Phe Val Hi s Val Leu Pr o 31u G31y Ala O31n Pro O31n Thr O31n Al a 30 gt t ct t ggc t cc aac at g t gc cac gt g cag gt a gaa at t gat gag gaa 259 Val Leu O31y Ser Asn rb t Cys Hi s Val O31n Val O31u Ilie Asp O31u O31u 45 gca ggc aaa gt c ct t gt t acc t cc gca at c gat aac ct c acc aag gga 307 Ala O31y Lys Val Leu Val Thr Ser Al a Ilie Asp Asn Leu Thr Lys O31y 60 act gcc ggc gcc gct gt t cag t gc at g aac t ta agc gt t ggt t tt gat 355 Thr Al a G31y Al a Al a Val (31n Cys [Vb t Asn Leu Ser Val G31y Phe Asp 75 80 gag gca gca ggc ct g cca cag gt c ggc gt c gca cct t aaagt agcg 401 O31u Al a Al a O31y Leu Pr o O31n Val O31y Val Al a Pr o ccttaaagcg gcg <210> 354 <211> 97 <21 2> PRT <213> Corynebact <400> 354 Leu Lys 03 u 03 y Phe Tyr Al a 03 n Pro O31n Thr O31n O3 u I I e Asp 03 u Asn Leu Thr Lys Ser Val 03 y Phe Pr o er i urn gI ut ani cumn Val Thr Ala Ou On O31u Thr Phe Val Hi s 25 Al a Val Leu O31y Ser 40 O3 u Al a 03 y Lys Val 55 O3 y Th r Al a 03 y Al a 70 As p 03 u Al a Al a 03 y Al a 10 ValI As n Le u Al a Le u 90 Ar g Le u vb t ValI ValI Pr o Al a Pr o Cys Thr O31n O31n ValI O31u Hi s Ser Cys ValI Tyr O31y ValI Al a vb t O31y O31u Al a O31n I Ie As n ValI <210O> 355 <21 1> 1302 <212> DNA <21 3> Cor ynebact er i urn gl ut anm cumn Page 460 seqi i st car r ect ed. t xt <220> <221> CDS <222> (101)..(1279) <223> RXS00147 <400> 355 attgcatata atgcaatgaa ttgaataaac tacattcagg ttttaaaaag gcagacacac gaaaggcgac aacagtcacc gt t at caacc agccaat t t c agt aaa gac Ser Lys Asp acc Thr gt t Val ggc G31y caa (31n acc Thr t cc Ser gca Al a at g lvb t ct g Leu t cc Ser 150 aag Lys gct Al a gt c Val t ct Ser t ac Tyr gca Al a acc Thr acc Thr cca Pr o gac Asp cgt Ar g ggc G31y 120 cgc Ar g gct Al a cag O31n gaa O31u gcc Al a 200 cgc Ar g gga G31y gga G31y ggt G31y acc Thr at c I Ie aag Lys t cc Ser ggc G31y ct g Leu gca Al a gca Al a 170 t ac Tyr gac Asp gt t Val acc Thr acc Thr gca Al a cct Pr o aac Asn t gg Tr p t gg Tr p gt c Val aat Asn 140 cgc Ar g acc Thr at c l Ie ggc G31y acc Thr 220 gag (31u ttc Phe gt g Val 45 t cc Ser acc Thr gt t Val cgc Ar g ggc G31y 125 gaa (31u cca Pr o ggc G31y gaa (31u at t l Ie 205 gt c Val at c l Ie acc Thr 30 ttc Phe t at Tyr ggc G31y gca Al a gcc Al a 110 at c l Ie ggt G31y gt t Val gca Al a gct Al a 190 aag Lys at c l Ie gga G31y 15 gga G31y act Thr cac Hi s t gg Tr p ggc G31y 95 acc Thr ggc G31y t cc Ser gaa (31u aac Asn 175 gaa O31u caa O31n gt g Val t cc Ser ttt Phe acc Thr cgc Ar g aac Asn 80 ct t Leu acc Thr gga G31y at t l Ie gaa O31u 160 ct c Leu ggc G31y aac Asn cct Pr o ccg Pr a ttt Phe at g lvb t at t l Ie gag O31u at c l Ie ttg Leu gac Asp 130 gcg Al a gt a Val gt t Val gag O31u cca Pr a 210 gaa O31u gca Al a gga G31y acc Thr gt t Val gac Asp cgc Ar g cag O31n 115 acc Thr ggc gy gag O31u gag O31u cgc Ar g 195 cgt Ar g acc Thr t ac Tyr gct Al a ggt G31y gt g Val aac Asn gac Asp 100 cag O31n cgc Ar g at c l Ie at c l Ie gt c Val 180 cac Hi s cgt Ar g cca Pr a acc Thr ct g Leu at c l Ie t ac Tyr gct Al a gag O31u ct c Leu gaa O31u gca Al a ttc Phe gt c Val 165 t ct Ser acc Thr ttc Phe ttc Phe Page 461 seql i st cor r ect ed. t xt gag gat atc aag cag tac aac cca tca ggc gtg ttc atc tcc aac ggc 835 Su Asp Ile Lys n Tyr Asn Pro Ser y Val Phe Ile Ser Asn y 230 235 240 245 cct ggc gat cct gca gca gca gac gtc atg gtt gat atc gtc cgc gaa 883 Pro G y Asp Pro Al a Al a Al a Asp Val Iet Val Asp I e Val Arg G u 250 255 260 gtt ctt gaa gcc gac att cca ttc ttt ggc atc tgc ttc ggc aac cag 931 Val Leu u Ala Asp lie Pro Phe Phe Gy Ile Cys Phe y Asn n 265 270 275 att ctt ggc cgc gca ttc ggc atg gag acc tac aag ctg aag ttc ggc 979 Ile Leu y Arg Ala Phe Gy IVet G u Thr Tyr Lys Leu Lys Phe y 280 285 290 cac cgc ggc atc aac gtt cca gtg aag aac cac atc acc ggc aag atc 1027 His Arg G y lie Asn Val Pro Val Lys Asn His lie Thr G y Lys IIe 295 300 305 gac atc acc gcc cag aac cac ggc ttc gca ctc aag ggt gaa gca ggc 1075 Asp IIe Thr Ala G n Asn His G y Phe Al a Leu Lys G y G u Ala G y 310 315 320 325 cag gaa ttc gag acc gat ttc ggc act gca att gtc acc cac acc tgc 1123 G n G u Phe G u Thr Asp Phe G y Thr Al a I e Val Thr His Thr Cys 330 335 340 ctc aac gac ggc gtc gtt gaa ggt att gcg ctg aag tcc gga cgc gca 1171 Leu Asn Asp y Val Val G u Gy Ile Ala Leu Lys Ser y Arg Ala 345 350 355 tac tcc gtt cag tac cac cca gag gcc gct gcc ggc cca aat gat gca 1219 Tyr Ser Val G n Tyr His Pro G u Ala Ala Ala G y Pro Asn Asp Ala 360 365 370 agc ccc ctg ttt gac cag ttt gtt gag ctg atg gat gca gac gct cag 1267 Ser Pro Leu Phe Asp G n Phe Val G u Leu IVet Asp Ala Asp Ala G n 375 380 385 aag aaa ggc gca taaataacat gccaaagcgt tca 1302 Lys Lys G y Al a 390 <210> 356 <211> 393 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 356 Val Ser Lys Asp Thr Thr Thr Tyr G n y Val Thr G u Ile y Ser 1 5 10 Val Pro Ala Tyr Leu Val Leu Ala Asp y Arg Thr Phe Thr y Phe 25 Sy Phe y Ala Ile y Thr Thr Leu y u Ala Val Phe Thr Thr 40 Ala IVet Thr G y Tyr G n G u Thr IVet Thr Asp Pro Ser Tyr His Arg 55 Sn Ile Val Val Ala Thr Ala Pro On lie Gy Asn Thr y Trp Asn 70 75 Asp G u Asp Asn u Ser Arg Asp G y Lys Ile Trp Val Ala G y Leu 90 Page 462 seqi i st car r ect ed. t xt Val lieArg Asp 1 00 Leu Ala Ala Arg Val Ser Asn Trp Arg A a Th r Th r 110 Ser I Ie A a 145 Le u Ser O31u Thr A a 225 Ph e As p cys Ly s I Ie 305 Ly s ValI Ly s O31y As p 385 O31n 115 Thr O31y O31u O31u Ar g 1 95 Ar g Thr Ser ValI O31y 275 Ly s O31y O31u Hi s O31y 355 As n As p O31u A a Ph e ValI 1 65 Ser Thr Ph e Ph e O31y 245 O31u O31n Oy le Oy 325 cys A a A a O31n A a ValI 135 G31y As n As p ValI A a 215 As p G31y Le u Le u Ar g 295 li1e (31u As n Ser Pr o 375 Ly s G31y 120 Ar g A a O31n O31u A a 200 Ar g I Ie As p O31u O31y 280 O31y Thr Ph e As p ValI 360 Le u (31y 1 05 O31n Hi s As p Pr o Thr 1 85 Tyr O31y Ly s Pr o A a 265 Ar g I Ie A a O31u O31y 345 O31n Ph e A a O31y Le u A a A a 1 70 Tyr As p ValI (31n A a 250 As p A a As n O31n Thr 330 ValI Tyr As p I Ie Ar g O31n 1 vb t ValI Le u Ar g Tyr 235 A a I Ie Ph e ValI As n 315 As p ValI Hi s O31n O31y 125 O31u Pr o O31y Ou le 205 ValI Pr a As p Ph e vb t 285 ValI O31y O31y O31y O31u 365 ValI O31y ValI A a A a 1 Ly s I Ie Ser ValI Ph e 270 O31u Ly s Ph e Thr I Ie 350 A a O31u O31y Ser O31u As n 1 O31u O31n ValI (31y vb t 255 O31y Thr As n A a A a 335 A a A a Le u <210O> 357 <21 1> 924 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (901) <223> RXS00905 Page 463 seqi i st car r ect ed. t xt <400> 357 cgct gcccct ct at gct gct cct agt tacc cct gcacaaa t agcggt t tt tct cacgcat t ct gcat cga gt cgggt cga cgt at at aag gt ggaaaggc at g acc caa t tc gaa N/t Thr 03 n Phe 03 u aac As n tt t Ph e cca Pr o ac a Thr gga G y aag Ly s g at As p c ac Hi s ct g Le u ac c Thr 1 50 ggc G3y gca Al a cca Pr o gt g ValI c aa O31n c ag O31n ct a Le u ac c Thr ac c Thr ct g Le u t gg Tr p ac c Thr 120 at g vb t ct c Le u gcg Al a gt c ValI tt g Le u 200 ac c Thr ct t Le u aaa Ly s gag O31u gca Al a tt c Ph e t ac Tyr ac c Thr t gg Tr p ct c Le u ct c Le u ag c Ser 1 70 tt g Le u act Thr g aa O31u aaa Ly s gaa O31u gac As p ggt O31y at c lIe 75 ag c Ser aat As n t ac Tyr gca Al a acc Thr 155 gcg Al a at t I e acc Thr ggc O31y gag O31u at t I Ie t ac Tyr ct c Le u ggc G31y c ac Hi s cct Pr o O31y gca Al a 140 t ac Tyr ct c Le u gca Al a ct g Le u gct Al a 220 at c I Ie t ct Ser ggc O31y gt c ValI cgc Ar g g ac As p g aa O31u 110 ggc O31y cgc Ar g gt c ValI aag Ly s ag c Ser 1 90 ggt O31y c ac Hi s g aa O31u 15 tt c Ph e gcg Al a ag c Ser aag Ly s gt t ValI 95 ct c Le u gcc Al a gcc Al a gag O31u g ac As p 1 75 gga O31y ggc O31y t cc Ser cgc Ar g aac As n aac As n aaa Ly s g aa O31u G31y 80 gt c ValI ac c Thr gct Al a gt c ValI O31y 1 60 aag Ly s aac As n gga O31y G31y gt t ValI 240 1 c aa O31n t cc Ser g aa O31u Ph e 65 t cc Ser cca Pr o gag O31u g ac As p g aa O31u 145 t cc Ser cct Pr a gct Al a c ag O31n c ag O31n 225 ct g Le u cgc Ar g gt g ValI t gg Tr p 50 gct Al a g aa G u t cc Ser cgc Ar g t gc cys 130 gcc Al a gag O3u gag O3u t cc Ser gt c ValI 210 aac As n g at As p gag O31u c ac Hi s 35 gt a ValI gcc Al a ggt G31y O31y g ac As p 115 aag Ly s ag c Ser g aa O31u ct t Le u gt g ValI 1 ac c Thr ggt O31y act Thr c ag O31n 20 ag c Ser aaa Ly s g aa O31u gca Al a cct Pr a 1 00 gct Al a ggc O31y ggc O31y at g vb t tt c Ph e 1 ggc O31y gt c ValI O31y Le u at c I Ie g at As p g aa O31u g at As p cca Pr a tt g Le u ggc O31y aac As n g ac As p gga O31y 1 g ac As p ac c Thr ac c Th r gct Al a cgc Ar g 245 gcc cca gat gct gt t gct gct ct c gt g Ala Pro Asp Ala Val Ala Ala Leu Val 230 235 Page 464 seql i st cor r ect ed. t xt gat gaa cac gga cgc acc gtt atc gac ggc tgt caa cac cac cgc aaa 883 Asp G u His Gy Arg Thr Val lie Asp Gy Cys n His His Arg Lys 250 255 260 ctg gaa ggg cga gcc tta tgatccagag actttccgca gcg 924 Leu G u G y Arg Al a Leu 265 <210> 358 <211> 267 <212> PRT <213> Corynebacter i um gl utam cum <400> 358 Mbt Thr G n 1 G n Arg G u Ser Val Hi s Su Trp Val Phe Al a Al a Ser G u G y Pro Ser G y Su Arg Asp 115 Asp Cys Lys 130 G u Al a Ser 145 Ser G u G u Pro G u Leu Al a Ser Val 195 G n Val Thr 210 Sn Asn y 225 Leu Asp Thr G n His Hi s <210> 359 Phe G u 5 Gn lle Ser Asp Lys G u Su Asp Ala Pr o Pro Leu 100 Al a G y Sy Asn Sy Asp lVt y 165 Phe Asp 180 G y Thr Val Thr G y Al a Leu Arg 245 Arg Lys 260 Asn Ala G n Val Phe Pr o Thr Gy 70 Lys Asp Hi s Leu Thr 150 Gy Al a Pr o Val Al a 230 Asp Leu Thr Asn Leu 55 Thr Val Leu Gy Val 135 Thr Gy Asp Thr Asp 215 Pr o G u G u Leu Leu Asn Asn Leu Asp 105 Ar g Hi s Asn Leu SIle 185 Thr Leu Al a Gy Ar g 265 Leu 10 Lys G u Al a Phe Tyr 90 Thr Tr p Leu Leu Ser 170 Leu Thr G u Val Ar g 250 Al a Lys G u Asp Gy SIle 75 Ser Asn Tyr Al a Thr 155 Al a SIle Thr Gy Al a 235 Thr Leu Su I e Tyr Leu Gy Hi s Pr o Gy Al a 140 Tyr Leu Al a Leu Al a 220 Al a Val Asn Val Al a Thr Thr Phe Phe Ar g 125 Leu Val SIle Asp Ar g 205 Val Leu SIle G u Phe Al a Ser Lys Val Leu Al a Al a G u Asp 175 Gy Gy Ser Ar g Gy 255 Asn Asn Lys G u Gy Val Thr Al a Val Gy 160 Lys Asn Gy Gy Val 240 Cys Page 465 seql i st cor r ect ed. t xt <211> 627 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(604) <223> RXS00906 <400> 359 accgtggaca cccttgaagg cgctgttcac tccggccaga gctgttgctg ctctcgtgcg cgttctggat actttgcgcg acggtggcgc tgccccagat ccg Pr o t at Tyr gac Asp cct Pr o ct c Leu cca Pr o ct g Leu gac Asp acc Thr acc Thr 150 gt c Val tcg Ser cca Pr o at g Met at c SIle gca Al a ggc Gy aat Asn aac Asn 120 gcg Al a ggc Gy cca Pr o gct Al a act Thr gac Asp at c SIle ccc Pr o gaa G u aca Thr ccg Pr o tgc Cys ggc Gy gt c Val tt c Phe ggc Gy acc Thr gca Al a 75 gca Al a cct Pr o tt c Phe ct g Leu gga G5y 155 aac Asn cgc Ar g gac Asp gga Gy acg Thr aac Asn tgg Tr p t cc Ser agc Ser 140 t cc Ser acc Thr agc Ser aac Asn 45 ttc Phe gcg Al a gat Asp ggc Gy acc Thr 125 gct Al a at t SIle acc Thr gat Asp cca Pr o act Thr t cc Ser gt g Val gca Al a 110 gat Asp gcg Al a cca Pr o gca Al a 15 gcc Al a gca Al a t cc Ser gcc Al a gcc Al a 95 aag Lys at t SIle t ac Tyr ct g Leu aac Asn ggc Gy agc Ser acc Thr aag Lys 80 gag G u at c SIle acc Thr gag Gu tgt Cys 160 at g Met 1 tgg Tr p at c SIle at g Met cca Pr o ct a Leu aag Lys acg Thr ggc Gy ggc Gy 145 acc Thr aac acg gac gca Asn Thr Asp Ala aag ggc gag cct Lys Gy G u Pro ctc gac ggt gta Leu Asp G y Val ctg tgg tcc agg Leu Trp Ser Arg gtg gca gaa gca Val Ala G u Ala aac ctt cgc gtg Asn Leu Arg Val ctg aag cag cac Leu Lys G n His 100 gtg gag atc gat Val u Ile Asp 115 cct gca atg tcc Pro Ala IVet Ser 130 aag gat ctt gtc Lys Asp Leu Val gaa ctg att gag G u Leu Ile G u 165 t aagcagaat t ggcact ct a cgg <210> 360 <211> 168 <212> PRT <213> Corynebacteri um gl utami cum <400> 360 IVet Asn Thr Asp Ala Pro Leu Ser Thr Ala Val Asn Thr Thr Ala Asn Page 466 seql i st cor r ect ed. t xt 1 5 10 Trp Lys Gy u Pro Tyr Asp Pro u Thr Phe Arg Ser Asp Ala Gy 25 Ile Leu Asp Gy Val Asp Ile Iet y Asp y Asp Asn Pro Ala Ser 40 IVet Leu Trp Ser Arg Pro Ala lie Ser Ile Thr y Phe Thr Ser Thr 55 Pro Val Ala G u Ala Leu Asn Ala Val Pro Ala Thr Ala Ser Ala Lys 70 75 Leu Asn Leu Arg Val Pro Ala y Leu G u Ala Asn Asp Val Ala G u 90 Lys Leu Lys G n His Leu I I e Asn His Thr Pro Trp G y Ala Lys I I e 100 105 110 Thr Val G u Ile Asp Asp Ile Asn G n Pro Phe Ser Thr Asp Ile Thr 115 120 125 G y Pro Al a IVet Ser Thr Leu Al a Ser Cys Leu Ser Al a Al a Tyr G u 130 135 140 Sy Lys Asp Leu Val Thr G u y Ser Gy y Ser Ile Pro Leu Cys 145 150 155 160 Thr u Leu lie u Val Asn Pro 165 <210> 361 <211> 246 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(223) <223> RXS00907 <400> 361 cctgagcgct gcgtacgagg gcaaggatct tgtcaccgaa ggcagcggcg gatccattcc actgtgtacc gaactgattg aggtcaaccc ataagcagaa ttg gca ctc tac ggt 115 Leu Ala Leu Tyr G y 1 gtg gaa gaa ccc ctc acc gtt atc cac tcc gct aat gaa tct gtt gac 163 Val G u G u Pro Leu Thr Val Ile His Ser Ala Asn u Ser Val Asp 15 ccc aat gag att cgc gat atc gcc acc gca gaa gca ttg ttc c ctgctc 211 Pro Asn u lie Arg Asp lie Ala Thr Ala u Ala Leu Phe Leu Leu 30 aac tac acc aag tagacccaaa agcaggcgtt aac 246 Asn Tyr Thr Lys <210> 362 <211> 41 <212> PRT <213> Cor ynebact er i um gl ut am cum Page 467 seqI i st car r ect ed. t xt <400> 362 Leu Ala Leu Tyr Gy Val Gu Gu Pro Leu Thr Val Ilie His Ser Ala 1 5 10 AsnOuSer Val Asp Pr oAs n 1u IlIe Ar gAsp Ile AlaThr Ala Ou 25 Al a Leu Phe Leu Leu Asn Tyr Thr Lys <210O> 363 <21 1> 1281 <21 2> DNA <21 3> Cor ynebact er i urn gI ut anm cumn <220> <221 CIDS <222> (1258) <223> RXS02001 <400> 363 gcggtt tcgt cat ggat aag gact gt gt tc gggaccatt g gat agt ccag cat agaccgt gct t tat cga aggt gaaccc cgat act cgt gt caaaaggc agt Ser cat Hi s gt g ValI at c I Ie ccc Pr o at t I Ie t gc cys ct g Le u cct Pr o gt c ValI 1 50 gcc Al a aac As n gag (31u aaa Ly s gt c ValI G31y c ac Hi s gag (31u 120 g aa (31u tt t Ph e g aa (31u ct g Le u ggt G31y ct g Le u g ac As p ggc G31y cgc Ar g aac As n ga As ag Se ag Se at 6 gc Al gc Al ac Th t t Ph gg a3 14 c gag at g acc p G3 u I'b t Thr 15 c t tt gat t gt r Phe Asp Cys c t tc ggg gt g r Phe G31y Val 45 c gcc ctg att e Al a Leu Il e 0 c gat at g gac a Asp I'b t Asp c t cg acc acc a Ser Thr Thr 95 g acc at g ct g r Thr I'b t Leu 110 c gca ggt acc e Al a G3 y Thr 125 C ggc gcg ggc y G3 y Al a G3 y 0 c gcc gaa gt c e Al a a3 u Val cgc Ar g gt g ValI g at As p c ac Hi s gcg Al a ccc Pr o ct g Le u gt c ValI gt t ValI t ac Tyr 1 at g vb t 1 t gg Tr p g aa a31u g aa a31u ggg a31y ct g Le u gga a31y ggc a31y gcg Al a at g vb t 145 gcc Al a ccc gt t at c Pro Val I Ie cgg Ar g act Thr at t I Ie cgc Ar g ccg Pr o aaa Lys5 gcc Al a ct g Le u 130 gt c ValI ct g Le u c ac Hi s gcc Al a ac c Thr gct Al a ac c Thr c ac Hi s 1 00 aaa tt c Ph e g aa a31u aac As n ct c gac cgc t tt gcc at Leu Asp Ar g Phe Al a 1 1 155 Page 468 ccc ggc ctg ccg Pro y Leu Pro ctt Leu 170 ggc cat tt G y His Ph seql i st cor r ect ed. t xt t atg acg aca gcc gg e Met Thr Thr Ala c ccg y Pro at c at g Ile bet 180 gcc gct gtc Al a Al a Val acg ttc gac atc Thr Phe Asp Ile aac Asn 190 att acc gga cgc lie Thr y Arg ggc ggc cac G y G y Hi s 195 gcc gtc gga Ala Val G y ggt gcc aaa ccg cac caa acc cgc gac ccc atc gtc gca G y Ala Lys Pro His G n Thr Arg Asp Pro IIe Val Al a 200 205 210 att gtc Ile Val 215 caa gcg ttt caa G n Al a Phe G n acg Thr 220 ata gtc agc cgg Ile Val Ser Arg cac aat ccg gtc His Asn Pro Val gag 230 gac ctt gtc gtg Asp Leu Val Val gtc acg caa atc Val Thr n lle cac Hi s 240 acc ggc agc gcc Thr G y Ser Ala aat atc atc ccc Asn Ile Ile Pro gaa G0u 250 acc gcc tat atc Thr Ala Tyr Ile ggc act gtc cgc Sy Thr Val Arg acc ttc Thr Phe 260 aac aaa gac Asn Lys Asp gcg ggc caa Al a G y G n 280 cag gcc atg gtc G n Al a Mvt Val at c Ile 270 acg cgg atg gaa Thr Arg Vbt G u gaa at c gt c G u Ile Val 275 acc tac aac Thr Tyr Asn gct gca gcc tat Al a Al a Al a Tyr gtc gag gcg acg Val G u Ala Thr ctg Leu 290 cgc aac tat ccc gcc acc att aac gac gcc gcc aaa gcc gcc atc gct Arg Asn Tyr Pro Ala Thr Ile Asn Asp Ala Ala Lys Ala Ala lle Ala 295 300 305 gcc Al a 310 gaa gtc gcg ggc G u Val Al a y gtc ggc ctc ggg Val y Leu y gtc Val 320 aac ccg aac ggc Asn Pro Asn y cgc ggg atg ggg Arg Gy Vb t y gcc Al a 330 gag gat ttc tcg G u Asp Phe Ser ttc ctc gaa aag Phe Leu G u Lys cgc ccg Arg Pro 340 ggt gcc tac G y Al a Tyr ccc gcc tat Pro Ala Tyr 360 ttc gtc ggt aat Phe Val y Asn ggc Sy 350 gac agc gcg ggc Asp Ser Al a G y aat ttc aac gac Asn Phe Asn Asp gcc gcg ccc tac Ala Ala Pro Tyr ggc G3y 370 ctt cac aac Leu Hi s Asn 355 gca tcg ttc Al a Ser Phe t gat ccat gg 1027 1075 1123 1171 1219 1268 1281 ttg gcc Leu Al a 375 cgc atg gca gaa Arg IVet Ala G u cgc Ar g 380 ccc ttg ccg tta Pro Leu Pro Leu aag ggc Lys G y 385 cgct cgaaga t gc <210> 364 <211> 386 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 364 IVet Pro Val lie Asn Ser lie Ala Ser 1 5 Phe Ser Asp Gu IVet Thr Arg 10 Page 469 seqi i st car r ect ed. t xt Trp Arg Arg His Leu His On Asn Pro Olu IlieSer Phe 25 Asp Cys Val O31u O31u G31y Le u G31y G31y A a vb t 145 A a A a G31y ValI As n 225 Thr Thr vb t Thr 305 As n Le u A a Tyr A a Hi s O31u Le u vb t A a 115 li1e As p Hi s Pr o G31y 1 95 A a As n Ser Ar g O31u 275 Thr A a As n Lys5 Le u 355 A a Ph e G31y O31y O31u A a Tyr O31n G31y O31n 1 65 vb t Hi s G31y ValI As p 245 Ph e ValI As n A a Ser 325 Pr o As n Ph e ValI I Ie Pr o 70 li1e Cy s Le u Pr o ValI 150 Pr a A a Gy le Ou 230 As n As n A a Ar g A a 310 Ar g G31y Pr a Le u A a A a ValI Thr G31y A a A a 135 Le u G31y A a A a ValI 215 As p I Ie Lys5 G31y As n 295 (31u G31y A a A a A a 375 O31n Thr O31y ValI As p 1 05 Thr O31u Ar g Pr a As p 1 85 Pr a A a ValI Pr a ValI 265 A a Pr a A a O31y Le u 345 As n Le u O31y Le u As p 90 O31y Ar g As n Ph e Le u 1 70 Thr Hi s Ph e ValI O31u 250 O31n A a A a O31y A a 330 Ph e Ph e Ar g I Ie Ar g Tyr Hi s As n O31y A a 1 O31y Ph e O31n O31n Ser 235 Thr A a A a Thr O31u 315 O31u ValI As n Ser lIe A a A a Thr Ph e O31y 140 li1e Hi s As p Thr Thr 220 ValI A a vb t Tyr I Ie 300 ValI As p O31y As p Ph e A a As p Ser Thr A a 125 O31y A a Ph e I Ie Ar g 205 li1e Thr Tyr ValI O31y 285 As n O31y Ph e As n O31u 365 O31y Le u vb t Thr vb t 110 O31y A a O31u vb t As n 1 As p ValI O31n I Ie I Ie 270 ValI As p Le u Ser O31y 350 A a ValI I Ie As p Thr Le u Thr O31y ValI Thr 1 li1e Pr a Ser I Ie As n 255 Thr O31u A a O31y Tyr 335 As p A a Ala (3u Ar g 380 Page 470 Pro Leu Pro Leu seql i st cor r ect ed. t xt Lys G y 385 <210> <211> <212> <213> <220> <221> <222> <223> 365 1386 DNA Cor ynebact eri um gl ut am cum CDS (101)..(1363) RXS02101 <400> 365 gccatggaat gctccgttga acgcaacagc cttaaataca agttttagtg tcgctgcgca ggtactctac tatctaatcc atcccctcct ataagccaag agc cgc att Ser Arg Ile gaa ctt cta aac G u Leu Leu Asn aat Asn cat ggt gtt gat His y Val Asp tcg tgg caa gag Ser Trp n u gcc gca Al a Al a t at cag gat Tyr G n Asp acc gca gat Thr Al a Asp cac gaa cat cct His G u His Pro gag u3 ctc tcc ggc ttc Leu Ser G y Phe gaa tca gag Su Ser G u tgt gag gtg Cys G u Val cgc att cag aaa Arg Ile n Lys ctc gag cgt ttt Leu G u Arg Phe gat Asp att cca Ile Pro aat gtt ggc ggt Asn Val G y y t ac Tyr ggc att ctg gcc Sy Ile Leu Al a ttc cga aat ggg Phe Arg Asn y tcg Ser aca gat cct ggt Thr Asp Pro Gy gcc Al a 75 cct gtt gcg tta Pro Val Ala Leu at g Met cgc gca gat ttc Arg Ala Asp Phe ggc ctt ccc gtc Sy Leu Pro Val aag Lys gaa atc acc gga G u I e Thr G y ccg ttt gct tcc Pro Phe Ala Ser act cgt Thr Arg 100 atg cgt ccg IVet Arg Pro cac gat gt c Hi s Asp Val 120 gat ggg gca aat Asp G y Ala Asn gt c Val 110 cat gtc atg cac His Val Met His gca tgc ggc Al a Cys G y 115 att t ta gat lIe Leu Asp cac gtc acc gcg His Val Thr Ala ctt ggt gcg tgt Leu G y Al a Cys gcc Al a 130 gag cgt G u Ar g 135 cgc gat gca tgg Arg Asp Ala Trp gaa G u 140 ggc acg ttc atc Gy Thr Phe lie ttg ttc cag cca Leu Phe G n Pro tcg Ser 150 gag gaa aac t cc G u G u Asn Ser caa G n 155 ggc gct aac aag G y Al a Asn Lys at g Met 160 gtc gcc ggc ggt Val Ala Gy y tta Leu 165 gtt gat ctg atc Val Asp Leu Ile cca Pr o 170 cgc cct gat gtg Arg Pro Asp Val ttt ggc cag cat Phe G y G n Hi s gta gtc Val Val 180 ccc ggt gct gca gga acc gtg atg agc Pro Gy Ala Ala Gy Thr Val Met Ser atg cct ggc ggt gct ctc gct IVet Pro Gy y Ala Leu Ala Page 471 seql i st cor r ect ed. t xt gcc tgc gat Al a Cys Asp 200 tcc att gaa atc Ser Ilie u Ile 190 att cag ggt cgc lie On y Arg 195 gcc cat ggt Al a Hi s G y agc Ser 210 t cc at g Ser 'bet 215 cct cat aat t cc Pro His Asn Ser at c SIle 220 gat ccc act tat Asp Pro Thr Tyr gca gcg atg att Ala Ala IVet Ile gt c Val 230 gtg cga ctc caa Val Arg Leu G n gga Gy 235 atc gtg ggc cgc I e Val y Arg gag G u 240 gtt tct cca gag Val Ser Pro G u ttc gcc gtt att Phe Al a Val I le tct Ser 250 gtg ggc acc ctc Val G y Thr Leu tcg ggc aac acc Ser G y Asn Thr aac aac Asn Asn 260 acc att cca Thr Ile Pro gac aaa gt c Asp Lys Val 280 agt gct cgt ttg Ser Al a Arg Leu gt g Val 270 ttg aac tgc cgt Leu Asn Cys Arg ttc tac aac Phe Tyr Asn 275 gtt gtc cgt Val Val Arg aag cac aag gtc Lys His Lys Val cga gcc atc gaa Arg Ala lie u cgt Ar g 290 ggt gaa y295 295 tgc ctt gct tcc Cys Leu Al a Ser ggt G3y 300 att gag gaa gaa lie u G u u gtc att gag tac Val Ile G u Tyr ttc Phe 310 gcc cac ggt gat Al a Hi s G y Asp acc aac aac acc Thr Asn Asn Thr cct Pr o 320 gtt gtc ttc gat Val Val Phe Asp gtg cgc cct gtc Val Arg Pro Val ttc Phe 330 gac gat gtt ttc Asp Asp Val Phe gag gat tct att Su Asp Ser lie gac gct Asp Al a 340 tac cgg tgg Tyr Arg Tr p aac agc cct Asn Ser Pro 360 gcg tcg gag gat Ala Ser G u Asp ttc Phe 350 ccc tcc att cct Pro Ser Ile Pro aag gca ttc Lys Ala Phe 355 cgc gat cag Arg Asp G n tac ctg tac tgg Tyr Leu Tyr Trp att ggt gtc acg lie y Val Thr ccg Pr o 370 1027 1075 1123 1171 1219 1267 1315 1363 1386 tgg aca Trp Thr 375 gaa gcc gta gaa Su Ala Val u aga Ar g 380 gac cgc gtg gca Asp Arg Val Ala tcg gat gtg cca gcc Ser Asp Val Pro Ala 385 ccg acg atg tcc gct Pro Thr IVet Ser Ala aat Asn 390 cac atg gga gat His Vet G y Asp ctc cct gat tat Leu Pro Asp Tyr gcg Al a 400 gcc acc cgc gca Ala Thr Arg Ala gcc Al a 410 gca gcc gcg ctg Al a Ala Ala Leu acc tac ttg gga Thr Tyr Leu G y act aac Thr Asn 420 taatcatcta gttttctgcg acg <210> 366 <211> 421 <212> PRT <213> Corynebacteri um gl utami cum <400> 366 IVet Ser Arg lie Ser G u Leu Leu Asn Asn His G y Val Asp Leu Ser Page 472 seql i st cor r ect ed. t xt Ala Tyr O n Asp 10 His G u His Pro Tr p G y Phe Val Ar g Phe vbt Cys Al a 145 Val Gy Gy Ar g Val 225 Val Gy Cys G u Pr o 305 Val Asp SIle G u G u Cys Ar g Asp Ser Al a 115 I le Phe Gy Hi s Al a 195 Al a Al a Pr o Thr Phe 275 Val SIle Phe SIle Lys 355 u Val Gy Asp Ar g Gy Asp Pr o Leu 165 Val Al a Gy l e Asp 245 Asn Asn Ar g Tyr Thr 325 Al a Phe Thr SIle Ser 70 Gy Met Hi s G u Ser 150 Val Pr o Al a Ser Val 230 Phe Thr Asp Gy Phe 310 Val Tyr Asn Al a Pr o Thr Leu Ar g Asp Ar g 135 G u Asp Gy Cys vbt 215 Val Al a SIle Lys G u 295 Al a Ar g Ar g Ser Phe Ar g Val Pr o Val Hi s 105 Hi s Asp Asn SIle Al a 185 Ser Hi s Leu l Ie Al a 265 Lys Leu Gy Val Thr 345 Tyr SIle Gy Gy Lys 90 Asp Val Al a Ser Pr o 170 Gy I e Asn G n Ser 250 Ser Hi s Al a Asp Phe 330 Al a n Gy Al a 75 G u Gy Thr Tr p G n 155 Ar g Thr G u Ser Gy 235 Val Al a Lys Ser Leu 315 Asp Ser Lys Tyr Pr o SIle Al a Al a G u 140 Gy Pr o Val SIle SIle 220 I le Gy Ar g Val Gy 300 Thr Asp G u Tyr Gy Val Thr Asn Leu 125 Gy Al a Asp Lb t Ar g 205 Asp Val Thr Leu Tyr 285 I le Asn Val Asp G u Leu Ser Leu G u Arg I e Leu Al a Al a Leu IVet G y Val Pro Val Hi s Val 110 Leu G y Al a Thr Phe Il e Asn Lys IVbt 160 Val Cys Phe 175 Ser IVbt Pro 190 lie Gn y Pro Thr Tyr G y Arg G u 240 Leu G n Ser 255 Val Leu Asn 270 Arg Ala lle Su Gu u Asn Thr Pro 320 Phe G y G u 335 Phe Pro Ser 350 Leu Tyr Trp Thr lie Gy Val 365 Page 473 seqI i st car r ect ed. t xt Thr ProArg Asp On TrpThr Gu AlaVal Ou Arg AspArg Val Al a 370 375 380 Ser Asp Val Pr o Al a Asn Hi s I'bt G31y As p Phe Leu Pro Asp Tyr Al a 385 390 395 400 Pr o Thr I'b t Ser Al a Al a Thr Ar g Al a Al a Al a Ala Ala Leu Leu Thr 405 410 415 Tyr Leu 03 y Thr Asn 420 <210O> 367 <21 1> 3462 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (3439) <223> RXS02234 <400> 367 acccagaggc cgct gccggc ccaaat gat g caagccccct t gat ggat gc agacgct cag aagaaaggcg cat aaat aac gt t tgaccag t t tgt tgagc at g cca aag cgt I'bt Pr o Lys Ar g g at As p c ag O31n aag at c I Ie gag (31u c ac Hi s aac As n gt t ValI cgc Ar g cgt Ar g aac As n t gt cys gag (31u ac c Th r g aa (31u at c I Ie gct Al a ct c Le u 120 aag Lys5 cgc Ar g gt c ValI tt c Ph e ct g Le u cca Pr a at c I Ie gcc Al a c ag O31n ggt G31y aag Lys5 t gc Cys ct c Le u gac As p cgc Ar g gaa O31u gac As p 75 gt c ValI ct g Le u gca Al a g at As p cac Hi s gt c ValI t ac Tyr gt c ValI at g vb t aag Lys5 ct g Le u g at As p g ac As p at t I Ie 140 aac As n at c I Ie t cc Ser ac c Th r 45 gct Al a at t I Ie gca Al a cgc Ar g at c I Ie 125 gt c ValI at g vb t ggt t cc ggc O3 y Ser 03 y 15 ggc acc cag O3 y Thr 03 n ct c at c aac Leu Il e Asn gac cac acc Asp Hi s Thr ttc gct aag Phe Al a Lys 80 acc ct t ggt Thr Leu G31y 95 ct c ggc at c Leu 03 y Il e 110 gat gcc at t Asp Al a Il e acc acc at c Thr Thr Ilie gaa gaa gtc 03 u 03 u Val 1 ccc Pr a gct Al a t cc Ser t ac Tyr g aa O31u G31y ct g Le u gag O31u ggt G31y 145 at c I Ie t gc cys aac As n gt g ValI at c I Ie c ag O31n g aa O31u cgc Ar g 130 ggc G31y gt c ValI cgc Ar g cca Pr a gag O31u gag O31u act Thr aag Lys5 115 ggc G31y g aa (31u at t lIe gt g ValI gca Al a cca Pr a c ag O31n gca Al a 1 00 t ac Tyr g aa O31u t cc Ser t ca Ser O31y ct g Le u ac g Thr at c O31y ct t Le u ggc O31y g at As p gcg Al a cac gag act gt c gca His Ou Thr Val Ala Page 474 seql i st cor r ect ed. t xt gaa G u ct g Leu ggt Gy t cc Ser gca Al a 230 ggc Gy act Thr cgc Ar g aac Asn tct Ser 310 aag Lys aac Asn t ac Tyr gct Al a t cc Ser 390 ct g Leu gca Al a ggc Gy t cc Ser gga Gy 200 ctt Leu aac Asn cac Hi s cgt Ar g gt c Val 280 gtt Val t cc Ser gct Al a at c SIle gt g Val 360 gac Asp ggc Gy acc Thr gag G u cca Pr o 170 ctt Leu gct Al a tgg Tr p gt g Val ggc Gy 250 ttc Phe gt g Val ggc Gy gct Al a aag Lys 330 ggt Gy aag Lys ttg Leu aac Asn cag G n 410 gct Al a gt a Val gca Al a gca Al a aag Lys gtt Val 235 gac Asp cag G n gac Asp cgc Ar g ct g Leu 315 ct g Leu gaa G u gcc Al a acc Thr tac Tyr 395 cag G n acc Thr gt c Val t ac Tyr tct Ser gaa G u 220 at c SIle tct Ser aag Lys acc Thr at c SIle 300 gca Al a gct Al a acc Thr cca Pr o acc Thr 380 at t SIle ggt Gy gat Asp gt g Val aac Asn cct Pr o 205 ttc Phe tgc Cys gt c Val at g Met ggt Gy 285 at c SIle t cc Ser at c l Ie cca Pr o cgc Ar g 365 acc Thr gca Al a ttc Phe aag Lys 160 cgt cca t cc Arg Pro Ser 175 acc gaa gac Thr G u Asp 190 gaa gca aac G u Al a Asn gag ctc gag G u Leu G u tcc att gaa Ser Ile G u 240 acc gtg gca Thr Val Al a 255 cgc gat cag Arg Asp n 270 gga t gt aac Sy Cys Asn acc att gag Thr Ile G u aag gca acg Lys Al a Thr 320 gga t ac acc G y Tyr Thr 335 gct gcg ttt Al a Al a Phe 350 ttt gct ttc Phe Al a Phe at g aag t cc IVet Lys Ser gca ct g aac Ala Leu Asn 400 tgg acc aag Trp Thr Lys 415 gca gct gtt Al a Al a Val act Thr gag G u ttg Leu 210 at g Met gt c Val gcc Al a at c SIle cag G n 290 aac Asn ttc Phe gat Asp ccc Pr o aag Lys 370 ggt Sy gca Al a gat Asp at g Met cgc Ar g 195 at c SIle cgc Ar g gac Asp ct g Leu gcc Al a 275 ttc Phe cca Pr o cca Pr o gag G u acc Thr 355 ttt Phe gag G u ct g Leu gag G u ggt G y 180 at c SIe gaa G u gat Asp gca Al a acc Thr 260 at c SIe gcc Al a cgt Ar g at t SIe at c SIe 340 at c SIe gt c Val gt c Val cgt Ar g ttc Phe 420 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 ctg gaa gat ctc aag Leu G u Asp Leu Lys Page 475 seql i st cor r ect ed. t xt cgc Ar g ggc Sy ttc Phe 470 gac Asp ggt Sy gaa G u ttc Phe t ac Tyr 550 gca Al a aac Asn gct Al a aac Asn ttc Phe 630 gcg Al a act Thr at t SIle ttc Phe acc Thr 440 agc Ser gcc Al a cca Pr o t cc Ser ggc Gy 520 act Thr t ac Tyr cag G n at c SIle gag G u 600 gag G u cca Pr o t cc Ser ct g Leu acc Thr 680 gca Al a ggc Gy gaa G u ctt Leu ct a Leu 490 ct g Leu cgc Ar g gat Asp gca Al a gag G u 570 cag G n t cc Ser gt g Val acc Thr acc Thr 650 ttg Leu cca Pr o ct g Leu cgc Ar g gaa G u gaa G u 475 aac Asn cag G n acc Thr acc Thr tac Tyr 555 cgt Ar g ggc Gy cgc Ar g t cc Ser tt c Phe 635 gt c Val gca Al a gag G u aac Asn ct c Leu ct c Leu 460 gct Al a gaa G u at c SIle ttg Leu tgt Cys 540 gag G u gaa G u at c SIle gt c Val acc Thr 620 gaa G u gca Al a gat Asp gca Al a cgc Ar g t ac Tyr 445 t ac Tyr ct c Leu gat Asp gca Al a cgt Ar g 525 gca Al a ct g Leu aag Lys gag G u ggc Gy 605 gac Asp gac Asp ggt Gy cgt Ar g at c l Ie 685 gag G u 430 gac gtt gag Asp Val G u gaa gca t ct G u Al a Ser gtg cag ttc Val G n Phe 480 ct c ct g cgc Leu Leu Arg 495 gcc ctt cgc Ala Leu Arg 510 ctg tcc ct a Leu Ser Leu gca gag ttt Al a G u Phe gat cca gca Asp Pro Al a 560 gt c ct g at c Val Leu Ile 575 ttc gac tac Phe Asp Tyr 590 tac gaa act Tyr G u Thr tac gac acc Tyr Asp Thr gtc atg gag Val Mvt G u 640 gtt atc gtc Val Ile Val 655 ttg aag aag Leu Lys Lys 670 gac atg gct Asp Ibet Al a cag ctt cct G n Leu Pro ct g Leu tct Ser 465 cgc Ar g gaa G u cca Pr o ggc Sy gaa G u 545 gct Al a ttg Leu t cc Ser gt c Val gct Al a 625 gt c Val cag G n gct Al a gag G u gca Al a 450 at t SIle cag G n gca Al a gag G u at c SIle 530 gct Al a gag G u ggc G y tgt Cys at g Met 610 gac Asp t ac Tyr ctt Leu ggc Sy gac Asp 690 cgc Ar g cct Pr o ct c Leu ttc Phe 500 gct Al a cca Pr o act Thr gag G u ggt G y 580 cac Hi s aac Asn ct g Leu gct Al a ggc Sy 660 cct Pr o ggc Sy ctt Leu tgg Tr p gtt Val 485 at g Met ggc Sy gt a Val ccg Pr o gt c Val 565 cca Pr o gca Al a tgc Cys t ac Tyr gag G u 645 cag G n gt c Val gag G u 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 gct cca gca ttc ggc Ala Pro Ala Phe y Page 476 seql i st cor r ect ed. t xt acc Thr 710 t ac Tyr gag G u act Thr aac Asn t ac Tyr 790 ggt Gy at c SIle gt a Val t ac Tyr t cc Ser 870 gt g Val gag G u gaa G u ct g Leu ggc Gy 950 acc Thr gt g Val gt c Val ttg Leu 760 at t SIle gca Al a t cc Ser aag Lys ggc Gy 840 at c SIle gca Al a gcc Al a gac Asp gt g Val 920 acc Thr gcc Al a ttc Phe gtt Val 730 gat Asp tct Ser at c SIle gt c Val tgt Cys 810 cgc Ar g at g Met gca Al a ggc Gy at c l Ie 890 ggc Gy ccg Pr o ctt Leu aac Asn gaa G u 715 cgc Ar g gag G u gac Asp gac Asp at g bet 795 gca Al a gaa G u aac Asn aac Asn gt c Val 875 aag Lys t cc Ser tt c Phe t cc Ser tt c Phe 955 700 gag G u cct Pr o gct Al a cac Hi s gt c Val 780 gag G u ctt Leu gca Al a gt c Val cca Pr o 860 aac Asn gat Asp ttg Leu aac Asn cca Pr o 940 ggc Gy gct Al a t cc Ser t cc Ser cca Pr o 765 gac Asp cac Hi s cct Pr o acc Thr cag G n 845 cgt Ar g ct g Leu ct c Leu cca Pr o cgc Ar g 925 gag G u gct Al a cgc Ar g t ac Tyr ct c Leu 750 gt g Val gca Al a at c SIle cca Pr o aag Lys 830 t ac Tyr gca Al a gcc Al a caa G n ct g Leu 910 ttc Phe at g Met gca Al a aca Thr gt c Val 735 gag G u ct g Leu ct g Leu gag G u at g Met 815 aag Lys gca Al a t cc Ser aag Lys gat Asp 895 gac Asp cgt Ar g aag Lys t at Tyr gt a Val 720 ttg Leu gat Asp gtt Val tgc Cys gaa G u 800 act Thr ct g Leu ct c Leu cgc Ar g gca Al a 880 gag G u gct Al a cgc Ar g t cc Ser gca Al a 960 705 gcc Al a ggt Gy t ac Tyr gac Asp gac Asp 785 gcc Al a ttg Leu gct Al a aag Lys acc Thr 865 gca Al a ggc Gy cca Pr o cca Pr o act Thr 945 aag Lys gat Asp ggc Gy at c SIle cgc Ar g 770 ggc Gy ggc Gy ggc Gy ct g Leu gac Asp 850 gt g Val t cc Ser at g let at c SIle gat Asp 930 ggc Gy gct Al a gag G u cgt Ar g aac Asn 755 ttc Phe gac Asp at t SIle gca Al a ggc Gy 835 gac Asp ccg Pr o cgt Ar g at t SIle gct Al a 915 gga Gy gag G u gaa G u at c SIe ggc Gy 740 cgc Ar g ct a Leu gag G u cac Hi s cag G n 820 at c SIe at c SIe ttc Phe at c SIe cct Pr o 900 gt g Val aag Lys gt c Val gct Al a 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 gcg ttt ggt gca ttg cca acc gaa ggc acc gtc Ala Phe Gy Ala Leu Pro Thr u Gy Thr Val ttc gtg acc gtg gct Phe Val Thr Val Ala Page 477 seqI i st car r ect ed. t xt 970 975 980 aac cgc gac aag cgc acc ctg atc ctg cca atc cag cgc ctg gcg ttg Asn Arg Asp Lys Arg Thr Leu Ilie Leu Pr o IIe O31n Ar g Leu Ala Leu 985 990 995 3091 at g vb t cgc Ar g g aa (31u 1 030 gt t Val gat As p at c I Ie ct g Leu 9gC (31y aac As n 101 E ggt (31 j ac As p J31y ac c Fh r cgt Ar g 1 09E t ac Ty r 1 000 ggc (31y gt a Val ct c Leu I t ac Ty r act Th r 1 080 gag (31u aag Lys~ at t I E g ag O31L at c Ile g at As p 1 06E gt t ValI ggC (31 at c I Ie gag ct c Le u 1 050 at c I Ie c ag O31n gt t Val ct C gcc acc Leu Al a Thr 1 005 t gt gaa gt t Cys (1u Val1 1 020 aag t cc at c Lys Ser Ilie 1 035 aac acc cca Asn Thr Pr o cgc gca gca Ar g Al a Al a ggt gt c acc G31y Val Thr 1 085 gt c agc gt c Val Ser Val 1100 g aa gt g ValI gt g ValI gct NI a gca NI a 070 gca Al a cgc Ar g ggc i31y ct c Le u gat As p ggt (31y 1 055 gt g ValI gct Al a gcg Al a ac c Th r aag Ly s cgt Ar g 1 040 t ct Ser ac c Th r gt c ValI ct g Le u gca Al a gct Al a 1 025 at c I Ie gct Al a gt g ValI c ag O31n c ag O31n 11 ggc at g G31y [vb t 1010 t cc gac Ser As p cgc gaa Ar g O31u ggc gct O3 y Al a ggt gt t O3 y Val 1 075 ggc at a G31y I Ie 1 090 gaa ct C 03 u Leu ct g Le u at c G31y cgc Ar g 1 060 ccg Pr o gag O31u g ac As p cgc 3139 Ar g cgc 3187 Ar g gaa 3235 1 045 cac 3283 Hi s ctg 3331 Leu gcc 3379 Al a cac 3427 Hi s gca gt c aag gct t aagccct at gacat tcggc gag Al a Val Lys Al a 1110 <210O> 368 <211> 1113 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 368 I'bt Pro Lys Ar g Ser Asp I Ie Asn Hi s Val Leu 1 5 10 Pro I Ie Val I Ie (1y (1n Ala Cys (1u Phe Asp AiaCy sAr gVal Leu Lys Ou Ou y LeuArg' 40 Ser As n Pr o Al a Thr I Ie I'b t Thr Asp Pro G31ui1 Ty r Val G31u Pr o I Ie G31u Pro G31u Ty r I Ie Asp 1 70 G31u I Ie (1u (1n (1y Hi s Pr o I Ie As pAla Val 1 90 O3 y 03 n Thr Al a Leu As n Al a Al a I I e 03 n Leu 100 105 Leu (3u Lys Ty r (3y Val (3u Leu I Ie (3y Al a 115 120 Page 478 3462 I Ie Ser Thr Al a I Ie Al a Ar g I Ie 125 Ser Thr I Ie Hi s Al a Le u G31y Al a seql i st cor r ect ed. t xt Su Arg Gy u Asp Ar g 130 G n Lys Phe Lys Asp lie Val Thr Thr Il e Gy 145 Hi s Phe Leu Val Leu 225 Asn Pr o Gy I e let 305 Gy Leu G u G u Val 385 Lys Pr o Leu Leu Ser 465 G u Thr bet Ar g 195 I le Ar g Asp Leu Al a 275 Phe Pr o Pr o G u Thr 355 Phe G u Leu G u Asp 435 let Asp Al a Al a 165 Gy Al a G u Thr Leu 245 Leu l e I e Val Al a 325 Thr Asp Gy lbt Ser 405 Phe Lys Leu Tr p Ar g 150 G u Leu Gy Ser Al a 230 Gy Thr Ar g Asn Ser 310 Lys Asn Ty r Al a Ser 390 Leu Al a Ar g Gy Phe 470 135 Ser Leu Gy Gy SIle 215 Asp Val Asp G u Pr o 295 Ar g lbt Asp Val Asp 375 Leu G u Gy Pr o Al a 455 Leu Ar g Gy Ser Gy 200 Leu Asn Hi s Ar g Val 280 Val Ser Al a SIle Val 360 Asp Gy Thr G u Thr 440 Ser Al a Val Leu Gy 185 Leu Gy Val Thr G u 265 Gy Asp Ser Al a Thr 345 Val Thr Ar g Lys Ar g 425 G u Val G u Cys Pr o 170 Leu Al a Tr p Val Gy 250 Phe Val Gy Al a Lys 330 Gy Lys Leu Asn G n 410 Al a Gy G u Leu Hi s 155 Val Al a Al a Lys Val 235 Asp G n Asp Ar g Leu 315 Leu G u Al a Thr Tyr 395 G n Thr Ar g Su Su 475 Asn Val Tyr Ser G u 220 I le Ser Lys Thr SIle 300 Al a Al a Thr Pr o Thr 380 I le Gy Asp Leu Leu 460 Al a Met Val Asn Pr o 205 Phe Cys Val Met Gy 285 I le Ser l Ie Pr o Ar g 365 Thr Al a Phe Lys Tyr 445 Tyr Leu G u Ar g Thr 190 G u G u Ser Thr Ar g 270 Gy Thr Lys Gy Al a 350 Phe lb t Al a Tr p Al a 430 Asp G u Val G u Pr o 175 G u Al a Leu SIe Val 255 Asp Cys SIe Al a Tyr 335 Al a Al a Lys Leu Thr 415 Al a Val Al a G n Arg G n Lys Leu Val Asp Ala Pro Phe Leu Asn G u Asp Leu Leu Arg Page 479 seqi i st car r ect ed. t xt 485 l'bt G3 y Leu Ser 490 Leu G3 n I I e Al a O31u Pr o G31y O31u 545 Al a Le u Ser ValI Al a 625 ValI O31n Al a O31u Al a 705 Al a G31y Tyr As p As p 785 Al a Le u Al a Ly s Ph e 515 Ar g Ly s Ser Ser ValI 595 ValI Ar g Hi s G31y ValI 675 Ar g Al a O31u Ar g As n 755 Ph e As p I Ie Al a G31y 835 (31y ValI Pr o ValI 565 Pr o Al a cys Tyr O31u 645 O31n ValI O31u G31y Ser 725 vb t Al a As p ValI Ser 805 As p G31y (31u Ph e Tyr 550 Al a As n Al a As n Ph e 630 Al a Thr I Ie Ph e Thr 71 0 Tyr O31u Thr As n Tyr 790 G31y I Ie ValI As p Ly s 535 Hi s Pr o Ar g Le u Pr o 615 O31u O31n Pr a G31y G31y 695 Al a Pr a I Ie O31u Al a 775 Le u As p O31u O31n As p 505 ValI ValI Ser Thr O31y 585 Le u Thr Le u O31y O31y 665 Ser Le u Ser Le u Tyr 745 Ser O31u O31y Al a ValI 825 Le u Thr Thr Tyr 555 Ar g O31y Ar g Ser Ph e 635 ValI Al a O31u As n O31u 715 Ar g O31u As p As p vb t 795 Al a O31u Le u cys 540 O31u O31u I Ie ValI Thr 620 O31u Al a As p Al a Ar g 700 O31u Pr a Al a Hi s ValI 780 O31u Le u Al a Ar g 525 Al a Le u Ly s O31u O31y 605 As p As p O31y Ar g I Ie 685 O31u Al a Ser Ser Pr a 765 As p Hi s Pr a Thr Al a 510 Le u Al a As p ValI Ph e 590 Tyr Tyr ValI ValI Le u 670 As p O31n Ar g Tyr Le u 750 ValI Al a I Ie Pr a Ly s 830 Le u Ser O31u Pr a Le u 575 As p O31u As p vb t I Ie 655 Ly s vb t Le u Thr ValI 735 O31u Le u Le u O31u vb t 815 Ly s Ar g Le u Ph e Al a 560 li1e Tyr Thr Thr O31u 640 ValI Ly s Al a Pr a ValI 720 Le u As p ValI cys O31u 800 Th r Le u l'bt Asn Val On Tyr Ala Leu 845 Page 480 Lys Asp 850 Asp Ile Leu Tyr Val II 855 seql i st cor r ect ed. t xt e G u Ala Asn Pro Arg 860 Al a Ser Arg Thr 865 Al a G y Pro I Pro Thr 945 Lys Phe G n Al a Al a 1025 lie Al a Val 3er Vet le Asp 930 Sy Al a Val Ar g Sy 101C Ser Ar g Sy Pro Phe Val Ser Lys 870 Arg I e Ala Val Gy 885 lie Pro Thr G u Tyr 900 Ala Val Lys G u Ala 915 Sy Lys Thr Leu Asp 935 G u Val e t G y Leu 950 G u Al a y Al a Phe 965 Thr Val Ala Asn Arg 980 Leu Ala Leu IVbt G y 995 IVet Leu Arg Arg Asn 101 Asp Ile Arg G u G 1030 Gu Gy u Val Asp 1045 Ala Arg His Asp G y 1060 Al a Al a Asp Val 920 Thr Al a Sy Asp Tyr 100C 5 Thr G y Val 875 Thr I e Lys 890 G y G y Ser 905 Leu Pro Phe Leu Leu Ser Asn Asn Phe 955 Ala Leu Pro 970 Lys Arg Thr 985 Lys Ile Leu y Ile u Cy Asn Leu Ala Lys Ala 880 Asp Leu G n Asp G u 895 Leu Pro Leu Asp Ala 910 Asn Arg Phe Arg Arg 925 Pro G u Iet Lys Ser 940 G y Al a Al a Tyr Al a 960 Thr G u G y Thr Val 975 Leu Ile Leu Pro Ile 990 Ala Thr G u y Thr 1005 's G u Val Val Leu Lys 1020 y Val u y Lys Se 1035 r lie Val Asp Arg 1040 I e Leu 1050 Asp Ile 1065 Thr Al a Al a y 1055 Al a Val 1070 Val y Val Pro Leu lie Thr Thr Val G n y Val Thr Ala Ala Val 1075 1080 1085 G n y Ile G u Ala Leu Arg G u Gy Val Val Ser Val Arg Ala Leu 1090 1095 1100 G n G u Leu Asp His Ala Val Lys Ala 1105 1110 <210> 369 <211> 3221 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(3198) <223> FRXA02234 <400> 369 ggc tcc aac cca gca acg atc atg acc gac cca gaa atg gct gac cac 48 G y Ser Asn Pro Ala Thr I e IVet Thr Asp Pro G u Iet Ala Asp His 1 5 10 acc tac gtg gag cca atc gag ccg gaa tac atc gac aag att ttc gct 96 Page 481 seql i st cor r ect ed. t xt Thr Tyr Val u Pro IIe G u Pro u Tyr Ile Asp Lys II e Phe Al a aag Lys ggt Gy at c SIle at t SIle at c l Ie gt c Val t cc Ser gac Asp 145 aac Asn gag G u gaa G u gca Al a cag G n 225 aac Asn gag G u acg Thr at c I e cag G n gaa G u cgc Ar g ggc Gy gag G u 115 act Thr gag G u ttg Leu at g bet gt c Val 195 gcc Al a at c SIle cag G n aac Asn ttc Phe 275 cag G n gca Al a t ac Tyr gaa G u t cc Ser gt c Val ggt Sy at c SIle gaa G u 165 gat Asp gca Al a acc Thr at c SIle gcc Al a 245 cgt Ar g at t SIle ggc G y ctt Leu ggc Gy 70 gat Asp gcg Al a gca Al a ggc G y gct Al a 150 gaa G u acc Thr ct g Leu ct g Leu at c SIle 230 at c l Ie gt g Val gcc Al a cac Hi s aac Asn gtt Val cgc Ar g cgt Ar g gaa G u ct g Leu 135 ggt G y t cc Ser gca Al a ggc G y act Thr 215 cgc Ar g aac Asn tct Ser aag Lys cca Pr o 40 gca Al a gaa G u cag G n t cc Ser ct c Leu 120 ggc G y ggc G y at c SIle gac Asp gt g Val 200 gac Asp gag G u cca Pr o cgt Ar g at g Met 280 at c SIle gct Al a ct c Leu aag Lys cgc Ar g 105 ggc Gy t cc Ser gga G y ctt Leu aac Asn 185 cac Hi s cgt Ar g gt c Val gtt Val t cc Ser 265 gct Al a gac Asp at c l Ie at c SIle ttc Phe 90 gt c Val ctt Leu ggt G y ctt Leu ggt G y 170 gtt Val acc Thr gaa G u ggc Gy gat Asp 250 t cc Ser gcc Al a gcc Al a cag G n ggt G y 75 aag Lys tgc Cys cca Pr o ctt Leu gct Al a 155 tgg Tr p gt g Val G y ttc Phe gt g Val 235 ggc G y gct Al a aag Lys gt c Val ct g Leu gca Al a gat Asp cac Hi s gt a Val gca Al a 140 gca Al a aag Lys gtt Val gac Asp cag G n 220 gac Asp cgc Ar g ct g Leu ct g Leu ct g Leu gat Asp gac Asp at t SIle aac Asn gt c Val 125 t ac Tyr tct Ser gaa G u at c SIle tct Ser 205 aag Lys acc Thr at c SIle gca Al a gct Al a 285 gca Al a cgc Ar g at c SIle gt c Val at g Met 110 gt g Val aac Asn cct Pr o ttc Phe tgc Cys 190 gt c Val at g Met ggt G y at c SIle t cc Ser 270 at c l Ie acc Thr ct c Leu gat Asp acc Thr gaa G u cgt Ar g acc Thr gaa G u gag G u 175 t cc Ser acc Thr cgc Ar g gga G y acc Thr 255 aag Lys gga G y ctt Leu ggc G y gcc Al a acc Thr gaa G u cca Pr o gaa G u gca Al a 160 ct c Leu att SIe gtg Val gat Asp tgt Cys 240 att SIe gca Al a t ac Tyr acc ctg gat gag atc acc aac gac atc act ggt gaa acc cca gct gcg Page 482 seql i st cor r ect ed. t xt Thr Leu Asp u Ile Thr Asn Asp Ile Thr Gy u Thr Pro Al a Al a 290 295 ttt Phe 305 ttc Phe t cc Ser aac Asn aag Lys gtt Val 385 gag G u tct Ser ttc Phe cgc Ar g cgc Ar g 465 ct a Leu ttt Phe gca Al a at c SIle t ac Tyr 545 ccc Pr o aag Lys ggt Gy gca Al a 355 gat Asp gaa G u gca Al a at t SIle cag G n 435 gca Al a gag G u at c SIle gct Al a gag G u 515 ggc Gy tgt Cys at c SIle gt c Val 325 gt c Val cgt Ar g ttc Phe ct c Leu cgc Ar g 405 cct Pr o ct c Leu ttc Phe gct Al a cca Pr o 485 act Thr gag G u ggt Gy cac Hi s gac Asp 310 ggc Gy at g Met t cc Ser tt c Phe aag Lys 390 ctt Leu tgg Tr p gtt Val at g bet ggc G y 470 gt a Val ccg Pr o gt c Val cca Pr o gca Al a 550 t ac Tyr gct Al a t cc Ser ct g Leu gca Al a 375 cgc Ar g G y ttc Phe gac Asp ggt G y 455 gaa G u ttc Phe t ac Tyr gca Al a aac Asn 535 gct Al a gt c Val gat Asp ct g Leu gaa G u 360 ggg Gy cca Pr o gca Al a ct c Leu gca Al a 440 ct g Leu gac Asp aag Lys cac Hi s cca Pr o 520 cgc Ar g ctt Leu gt g Val gac Asp ggc Gy 345 acc Thr gag G u acc Thr agc Ser gcc Al a 425 cca Pr o t cc Ser ggc G y act Thr t ac Tyr 505 cag G n at c SIle gag G u aag Lys 315 ttg Leu aac Asn cag G n gct Al a ggc Gy 395 gaa G u ctt Leu ct a Leu ct g Leu cgc Ar g 475 gat Asp gca Al a gag G u cag G n t cc Ser 555 300 gcc Al a acc Thr t ac Tyr cag G n acc Thr 380 cgc Ar g gaa G u gaa G u aac Asn cag G n 460 acc Thr acc Thr t ac Tyr cgt Ar g ggc G y 540 cgc Ar g cca Pr o acc Thr at t SIle ggt G y 365 gat Asp ct c Leu ct c Leu gct Al a gaa G u 445 at c SIle ttg Leu tgt Cys gag G u gaa G u 525 at c SIle gt c Val cgc Ar g acc Thr gca Al a 350 ttc Phe aag Lys t ac Tyr t ac Tyr ct c Leu 430 gat Asp gca Al a cgt Ar g gca Al a ct g Leu 510 aag Lys gag gcu ggc Gy ttt Phe at g Met 335 gca Al a tgg Tr p gca Al a gac Asp gaa G u 415 gt g Val ct c Leu gcc Al a ct g Leu gca Al a 495 gat Asp gt c Val ttc Phe t ac Tyr gct Al a 320 aag Lys ct g Leu acc Thr gct Al a gtt Val 400 gca Al a cag G n ct g Leu ctt Leu t cc Ser 480 gag G u cca Pr o ct g Leu gac Asp gaa G0u 560 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 act gtc atg gtc aac tgc aac cca gag acc gtg tcc acc gac tac gac Page 483 seql i st cor r ect ed. t xt Thr Val let Val Asn Cys Asn Pro u Thr Val Ser Thr Asp Tyr Asp 575 565 acc Thr gag G u gt c Val aag Lys 625 gct Al a cct Pr o gt a Val ttg Leu gat Asp 705 gtt Val tgc Cys gaa G u act Thr ct g Leu 785 ct c Leu cgc Ar g gac Asp t ac Tyr 595 ctt Leu ggc Gy gac Asp cca Pr o gat Asp 675 ggc G y at c SIle cgc Ar g ggc G y ggc Gy 755 ggc Gy ct g Leu gac Asp gt g Val ct g Leu gct Al a ggc Gy cct Pr o ggc Gy 645 ttc Phe at c SIle ggc G y cgc Ar g ct a Leu 725 gag G u cac Hi s cag G n at c SIle at c SIle 805 ttc Phe tac Tyr gag G u cag G n gt c Val 630 gag G u ggc G y agc Ser at g Met gca Al a 710 gac Asp gt c Val t cc Ser gac Asp ggt G y 790 ct c Leu gt c Val ttc Phe gcg Al a act Thr 615 at t SIle ttc Phe acc Thr t ac Tyr gag G u 695 act Thr aac Asn t ac Tyr ggt G y at c SIle 775 gt a Val t ac Tyr t cc Ser gag G u cag G n 600 cct Pr o ggt Gy ggt Gy gca Al a cca Pr o 680 at t SIle gag G u gct Al a ct g Leu gac Asp 760 gag G u cag G n gt c Val aag Lys cca Pr o 585 t cc Ser ct g Leu acc Thr gca Al a acc Thr 665 gt g Val gt c Val ttg Leu at t l Ie gca Al a 745 t cc Ser aag Lys ggc G y at c SIle gca Al a 825 570 ct g Leu ggc G y ggc G y t cc Ser ct g Leu 650 tct Ser ct g Leu t ac Tyr tct Ser gag G u 730 ggc G y gca Al a gt c Val ct g Leu gag G u 810 acg Thr acc Thr acc Thr ttg Leu cca Pr o 635 ct g Leu ttc Phe gtt Val gat Asp tct Ser 715 at c SIle gt c Val tgt Cys cgc Ar g at g Met 795 gca Al a ggc G y ttc Phe gt c Val gca Al a 620 gag G u aac Asn gaa G u cgc Ar g gag G u 700 gac Asp gac Asp at g let gca Al a gaa G u 780 aac Asn aac Asn gt c Val gaa G u gca Al a 605 gat Asp gca Al a cgc Ar g gag G u cct Pr o 685 gct Al a cac Hi s gt c Val gag G u ctt Leu 765 gca Al a gt c Val cca Pr o aac Asn gac Asp 590 ggt G y cgt Ar g at c l Ie gag G u gct Al a 670 t cc Ser t cc Ser cca Pr o gac Asp cac Hi s 750 cct Pr o acc Thr cag G n cgt Ar g ct g Leu 830 gt c Val gtt Val ttg Leu gac Asp cag G n 655 cgc Ar g t ac Tyr ct c Leu gt g Val gca Al a 735 at c SIe cca Pr o aag Lys t ac Tyr gca Al a 815 gcc Al a 1776 1824 1872 1920 1968 2016 2064 2112 2160 2208 2256 2304 2352 2400 2448 2496 2544 gca gca tcc cgt atc gca gtg ggc gcc acc atc aag gat ctc caa gat Page 484 Ala Ala Ser Arg 835 seql i st cor r ect ed. t xt Ile Ala Val Gy Ala Thr Ile Lys Asp 840 845 Leu G n Asp gag G u gct Al a 865 cgc Ar g t cc Ser gca Al a gt c Val at c SIle 945 acc Thr aag Lys cgt Ar g at g Met at c l Ie gat Asp ggc Gy gct Al a 915 gt g Val cgc Ar g ggc G y t cc Ser cgc Ar g 995 cct Pr o gt g Val aag Lys 885 gt c Val gct Al a gt g Val gcg Al a ct g Leu 965 at c SIle ggc G y acc Thr aag Lys 870 acc Thr at g Met ggc Gy gct Al a tt g Leu 950 cgc Ar g cgc Ar g gaa Gu gag G u 855 gaa Gu ct g Leu ggc Gy gcg Al a aac Asn 935 at g Met cgc Ar g gaa G u gtt Val tac Tyr gca Al a gac Asp ttg Leu c ttt Phe 920 cgc Arg ggc t G y aac Asn ggt G y c gac Asp 1000 gac Asp gtg /al acc Thr gcc %I a 305 ggt 3 y gac Asp ac Tyr ggc G y gt a /al 385 ggc G y ttg Leu ct g Leu 890 aac Asn gca Al a aag Lys aag Lys at t SIle 970 gag G u ggc G y ccg Pr o 875 ctt Leu aac Asn ttg Leu cgc Ar g at c SIle 955 gag G u ggc G y t cc Ser 860 ttc Phe t cc Ser ttc Phe cca Pr o acc Thr 940 ct c Leu tgt Cys aag Lys ttg Leu aac Asn cca Pr o ggc Gy acc Thr 925 ct g Leu gcc Al a gaa G u t cc Ser cca Pr o cgc Ar g gag G u gct Al a 910 gaa G u at c SIle acc Thr gtt Val at c SIle 990 ct g Leu ttc Phe at g Met 895 gca Al a ggc Gy ct g Leu gaa G u gt g Val 975 gt g Val 2592 2640 2688 2736 2784 2832 2880 2928 2976 3024 ct c at c ct c Leu Ile Leu aac acc cca gct ggt Asn Thr Pro Ala G y 1005 tct gct ggc gct cgc cac gat ggc Ser Ala Gy Ala Arg His Asp G y 1010 1015 acc gtg ggt gtt ccg ctg atc acc Thr Val y Val Pro Leu lie Thr 1025 1030 gtc cag ggc ata gag gcc ctg cgt Val n Gy Ile u Ala Leu Arg 1045 ctg cag gaa ctc gac cac gca gtc Leu n G u Leu Asp His Ala Val 1060 gag <210> 370 <211> 1066 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 370 t ac Tyr act Thr gag 3 u aag Lys 1065 gat Asp gtt Val ggc Gy 1050 gct Al a atc cgc gca gca gca gtg 3072 IIe Arg Ala Ala Ala Val 1020 cag ggt gtc acc gca gct 3120 Sn Gy Val Thr Ala Ala 1035 1040 gtt gtc agc gtc cgc gcg 3168 Val Val Ser Val Arg Ala 1055 taagccctat gacattcggc 3218 3221 Page 485 G31y Thr Ly s G31y I Ie li1e I Ie ValI Ser As p 145 As n O31u O31u A a O31n 225 As n O31u Thr Thr Ph e 305 Ph e Ser As n Ser Asn Pro Ala Thr Ilie I'bt eqI i st car r ect ed. t xt Thr Asp Pro Ou Ntbt Ala Asp His 10 ValI I Ie O31n O31u Ar g O31y O31u 115 Thr O31u Le u vb t ValI 1 95 A a I Ie O31n As n Ph e 275 As p Pr o Lys5 G31y A a 355 Pr o O31n A a Tyr O31u Ser ValI O31y I Ie O31u 1 65 As p A a Thr I Ie A a 245 Ar g I Ie I Ie I Ie ValI 325 ValI Ar g I Ie O31y Le u O31y 70 As p A a A a O31y A a 150 O31u Thr Le u Le u I Ie 230 li1e ValI A a Thr As p 310 O31y vb t Ser O31u Hi s As n ValI Ar g Ar g O31u Le u 135 O31y Ser A a O31y Thr 215 Ar g As n Ser Lys5 As n 295 Tyr A a Ser Le u O31u li1e A a Le u Lys5 Ar g 1 05 O31y Ser O31y Le u As n 1 85 Hi s Ar g ValI ValI Ser 265 A a I Ie ValI As p O31y 345 Thr I Ie A a O31n O31y 75 Lys5 cys Pr o Le u A a 1 55 Tr p ValI O31y Ph e ValI 235 O31y A a Lys5 O31y Ly s 315 Le u As n As p ValI Le u A a As p Hi s ValI A a 140 A a Ly s ValI As p O31n 220 As p Ar g Le u Le u O31u 300 A a Thr Tyr Ly s Le u As p As p I Ie As n ValI 125 Tyr Ser O31u I Ie Ser 205 Ly s Thr I Ie A a A a 285 Thr Pr o Thr I Ie lIe A a Ar g I Ie ValI vb t 110 ValI As n Pr a Ph e cys 1 ValI vb t Oy le Ser 270 li1e Pr a Ar g Thr A a 350 Ph e Thr Le u As p Thr O31u Ar g Thr O31u O31u 1 Ser Thr Ar g O31y Thr 255 Ly s O31y A a Ph e vb t 335 A a A a Le u O31y A a Thr O31u Pr a O31u A a 1 Le u I Ie ValI As p cys 240 li1e A a Tyr A a A a 320 Ly s Le u Lys On On Gy Phe Trp Thr 365 Page 486 seql i st cor r ect ed. t xt Lys Pro Asp u Phe Phe Ala Gy u Arg Ala Thr 370 375 380 Asp Lys Ala Ala Val 385 G u Ser Phe Ar g Ar g 465 Leu Phe Al a SIle Tyr 545 Thr Thr G u Val Lys 625 Al a Pr o Val Leu Asp 705 G u Al a SIle G n 435 Al a G u SIle Al a G u 515 Gy Cys lbt Asp Tyr 595 Leu Gy Asp Pr o Asp 675 Gy lie Leu Ar g 405 Pr o Leu Phe Al a Pr o 485 Thr G u Gy Hi s Asn 565 Leu Al a Gy Pr o Gy 645 Phe SIle Gy Ar g Ar g Gy Phe Asp Gy 455 G u Phe Tyr Al a Asn 535 Al a Asn Phe Al a Thr 615 I le Phe Thr Tyr G u 695 Thr Pr o Al a Leu Al a 440 Leu Asp Lys Hi s Pr o 520 Ar g Leu Pr o G u G n 600 Pr o Gy Gy Al a Pr o 680 I le G u Thr Ser Al a 425 Pr o Ser Gy Thr Tyr 505 G n SIle G u G u Pr o 585 Ser Leu Thr Al a Thr 665 Val Val Leu Gy 395 G u Leu Leu Leu Ar g 475 Asp Al a G u G n Ser 555 Val Thr Thr Leu Pr o 635 Leu Phe Val Asp Ser 715 Leu Leu Al a G u 445 I le Leu Cys G u G u 525 I le Val Thr G u Al a 605 Asp Al a Ar g G u Pr o 685 Al a Hi s Tyr Tyr Leu 430 Asp Al a Ar g Al a Leu 510 Lys G u Sy Asp Asp 590 Sy Ar g l Ie G u Al a 670 Ser Ser Pr o Asp G u 415 Val Leu Al a Leu Al a 495 Asp Val Phe Tyr Tyr 575 Val Val Leu Asp G n 655 Ar g Tyr Leu Val Val Asp Arg Phe Leu Asp Asn Ala lie G u lie Asp Val Asp Ala Leu Page 487 seql i st cor r ect ed. t xt 725 G u Val Tyr Leu Cys G u Thr Leu 785 Leu Ar g Al a G u Al a 865 Ar g Ser Al a Val SIle 945 Thr Lys Gy Gy 755 Gy Leu Asp Val Ser 835 Ibet l Ie Asp Gy Al a 915 Val Ar g Gy Ser Hi s G n I e I e 805 Phe SIle Pr o Val Lys 885 Val Al a Val Al a Leu 965 I le Gy I e 775 Val Tyr Ser Val G u 855 G u Leu Gy Al a Asn 935 Ibet Ar g G u 730 Al a y Val 745 Ser Al a Cys Lys Val Arg G y Leu IVet 795 l e G u Al a 810 Al a Thr G y 825 Ala Thr Ile Asp G y y Val Leu Pro 875 Thr Leu Leu 890 Al a Asn Asn 905 G y Al a Leu Asp Lys Arg Tyr Lys Ile 955 G y lie u 970 Val u y 985 MVt u His 750 Ala Leu Pro 765 G u Al a Thr 780 Asn Val n Asn Pro Ar g Val Asn Leu 830 Lys Asp Leu 845 Ser Leu Pro 860 Phe Asn Arg Ser Pro G u Phe G y Al a 910 Pro Thr G u 925 Thr Leu Ile 940 Leu Al a Thr Cys G u Val Lys Ser Ile 990 735 Ile u Pro bet Lys Lys Tyr Al a 800 Al a Ser 815 Al a Lys Sn Asp Leu Asp Phe Arg 880 IVet Lys 895 Al a Tyr G y Thr Leu Pro G u G y 960 Val Leu 975 Val Asp Arg II e Ar g 995 Ser Ala G y 1010 Thr Val G y 1025 Val Gn Gy I Leu G n G u <210> 371 <211> 1389 3 u G y G u Val Asp 1000 Leu Ile Leu Asn Thr Pro Ala G y 1005 Al a Ar g Val Pr o l e u 1045 Leu Asp 1060 His Asp y 1015 Leu I e Thr 1030 Ala Leu Arg Hi s Al a Val Tyr Asp Thr Val G u G y 1050 Lys Al a 1065 IIe Arg Ala Ala Ala 1020 G n y Val Thr Al a 1035 Val Val Ser Val Arg 1055 Val Al a 1040 Al a Page 488 seqi i st car r ect ed. t xt <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1366) <223> RXS02565 <400> 371 ggaaat tcga t acagt gcga t gacgcgat a t tagaaagaa aaagat gcgc t t tacgacga aaccct cacc ct cct t cagg aact t at ccg caacgcct gc cca Pr o tt t Ph e ggc G31y gag O31u cct Pr o at t I Ie gcg A a ct g Le u gcg A a t gc CYS 1 50 g ac As p at t I Ie g ac As p t ca Ser gga G31y ac c Thr tt a Le u t gg Tr p ggt G31y gt c ValI tt c Ph e 120 t gg Tr p t cc Ser gt a ValI gt c ValI gct A a 200 c ag O31n ccc Pr o at t I Ie ct g Le u aaa Lys ggg G31y cgt Ar g ggc G31y t cc Ser t cc Ser at t I Ie 1 70 ggc G31y gt c ValI gaa O31u aac As n at c I Ie ct t Le u g at As p 75 t cc Ser caa O31n gt t ValI gaa O31u ggt G31y 155 aac As n g at As p aag Lys at t I Ie gt t ValI gt g ValI gga G31y cca Pr o gt c ValI gt a ValI gct A a g aa O31u 140 gga G31y gt t ValI gct A a at c I Ie ag a Ar g aaa Lys act Thr 45 cat Hi s tt c Ph e g at As p gcc A a g at As p 125 c ac Hi s t cg Ser gga G31y ggt G31y ggt G31y 205 aac As n at c I Ie gt t ValI act Thr ggt G31y at g vb t cgt Ar g 110 gag O31u c aa O31n c ac Hi s g aa (31u cat Hi s 1 90 g aa (31u gcg A a 15 ac c Thr cca Pr a g at As p gcg A a ct c Le u 95 g aa (31u g aa (31u aac As n ct t Le u aaa Lys 1 75 ggt G31y gt c ValI g aa (31u aag Lys ggc G31y gt t ValI gag (31u 80 tt t Ph e ggc G31y gcc A a ct c Le u cca Pr a 1 60 ggt G31y t cc Ser gcc A a gt g ValI 1 ag c Ser ct g Le u ag c Ser gt g ValI 65 at t I Ie at t I Ie ggc G31y cgc Ar g tt c Ph e 145 gt c ValI gca A a at t I Ie cgc Ar g aat gat As n As p cta gaa Leu 03 u gaa ccg O31u Pr o 35 gat cca As p Pr o 50 cct gt t Pr o Val t cg gat Ser As p acc gca Thr A a ct g cgt Leu Arg 115 ggc gga G31y (1y 130 agc t gg Ser Tr p cac gac Hi s As p gct caa A a 03 cct tt c Pr o Phe 1 cga at c Ar g I Ie ct a acc Leu Thr cgt Ar g 20 cat Hi s g at As p g at As p gga G31y ac c Thr 1 00 ggc G31y ct c Le u aaa Lys ggc G31y cgt Ar g 1 g ac As p gct A a tt c Ph e ccg Pr a gct A a ct g Le u c ag O31n c aa O31n ac g Thr gga O31y aac As n ag c Ser 1 cgt Ar g cgt Ar g gcc A a 210 gcc gat ct gaag gt agcc aag gac gat at c tgg caa ggct t cgt ccaa Page 489 Al a Asp 215 Leu Lys Val Al a seql i st cor r ect ed. t xt Lys Asp Asp lie Trp On Gy 220 225 Phe Val G n gcg Al a 230 t cc Ser gcg Al a gcc Al a acc Thr gct Al a 310 gaa G u gaa G u at t SIle gtt Val gca Al a 390 ctt Leu cgt Ar g gag G u tct Ser aat Asn 280 cca Pr o ggc Gy gca Al a gtt Val tct Ser 360 t at Tyr ggg G y ct g Leu gac Asp t ac Tyr 250 ct c Leu ttg Leu caa G n ctt Leu gt g Val 330 ggt Sy ggc G y ttt Phe ctt Leu gtt Val 410 cca Pr o 235 gca Al a acg Thr cca Pr o acc Thr gcc Al a 315 agc Ser gat Asp tct Ser gca Al a cac Hi s 395 cgg Ar g gaa G u gag G u at c SIle tcg Ser aat Asn 300 gat Asp cca Pr o ttc Phe gac Asp gtt Val 380 t cc Ser ggt Gy gag cag G u G n ggc gga G y G y 255 caa act G n Thr 270 gcg tac Al a Tyr tat gtt Tyr Val gta gaa Val u gat t cc Asp Ser 335 ccc gat Pro Asp 350 cgc ttt Arg Phe gca cgt Al a Ar g gac gag Asp u gac t cc Asp Ser 415 gcg Al a 240 ct c Leu gt g Val ttg Leu gat Asp at c SIle 320 agg Ar g gcg Al a ggt G y gaa G u gcg Al a 400 ctt Leu cgc Ar g cgt Ar g ct g Leu 290 acc Thr cac Hi s t at Tyr gt g Val cga Ar g 370 act Thr t ac Tyr agc Ser ttc Phe gca Al a 275 gat Asp ct g Leu ct c Leu aac Asn gt c Val 355 ct a Leu ttg Leu ct g Leu ggg acc G y Thr 245 gcc cac Al a Hi s 260 ggt caa G y G n at c cgt li e Arg cgt gct Arg Ala at c t ct Ile Ser 325 acc ttg Thr Leu 340 cca att Pro Ile ggc ggt G y G y gcg gaa Al a u gaa gat G u Asp 405 ttc cta Phe Leu 420 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1389 gtc gtg cgt gaa Val Val Arg G u ggc taaaaacatg aagcaggagt ctt Gy <210> 372 <211> 422 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 372 Val Asn Asp Leu Thr Pro Asp Ser G y On u lle Arg Asn Al a u 1 5 10 Ser Leu G u Arg Phe Phe G u Gy Thr Pro Asn Val Lys Ile Thr Lys 25 Page 490 Leu Ou Pro His Pro Oy Arg Thr 40 eqI i st car r ect ed. t xt Ser Ilie IlieVal Thr Val Pro O31y Ser ValI li1e I Ie O31y Ar g Ph e 145 ValI A a I Ie Ar g O31n 225 Le u Ser ValI O31u As p 305 O31u Le u Pr o Ar g Ar g 385 Pr o ValI As p A a Ar g 115 O31y Tr p As p O31n Ph e 1 95 li1e Ph e Ser Ph e A a 275 As p Le u Le u As n ValI 355 Le u Le u A a Le u O31n O31n Thr O31y As n Ser 1 65 Ar g Ar g A a O31n Thr 245 Hi s O31n Ar g A a Ser 325 Le u I Ie O31y (31u O31u Pr o 70 li1e A a Le u A a Cy s 150 As p I Ie As p A a A a 230 Ser A a A a Thr A a 310 O31u O31u I Ie ValI A a 390 Pr o Lys5 Tr p A a Thr Lys5 135 Le u A a Hi s Ser As p 215 Hi s Pr a ValI Le u 295 Le u O31u Lys5 Ser O31y 375 Le u Tr p O31y ValI Ph e 120 Tr p Ser ValI ValI A a 200 Le u Ar g O31u Ser As n 280 Pr a O31y A a ValI Ser 360 Tyr (31y Thr Thr Ar g Thr 1 05 ValI Le u O31u ValI As n 1 85 li1e Lys5 Ph e A a Hi s 265 ValI O31y As p Thr Le u 345 O31y O31y (31n Le u As p 75 Ser O31n ValI O31u O31y 1 55 As n As p Ly s A a Pr a 235 A a Thr Pr a Thr A a 315 Ser As p Ser A a Hi s Ph e As p A a As p 125 Hi s Ser O31y O31y O31y 205 As p Thr Ph e A a Hi s 285 As p O31u Thr Ph e Le u 365 Hi s Thr O31y vb t Ar g 110 O31u O31n Hi s O31u Hi s 1 O31u As p O31u O31y O31n 270 A a Tyr ValI As p Pr a 350 Ar g A a As p A a Le u O31u O31u As n Le u Ly s 1 O31y ValI I Ie O31n O31y 255 Thr Tyr ValI O31u Ser 335 As p Ph e Ar g ValI O31u Ph e O31y A a Le u Pr a 1 O31y Ser A a Tr p A a 240 Le u ValI Le u As p I Ie 320 Ar g A a O31y O31u Leu His Ser His Asp 3u Ala 395 400 Page 491 seqi i st car r ect ed. t xt Leu Tyr Leu O31u Asp Leu O31u Leu Thr Val Ar g G31y Tyr Asp Ser Val 405 410 415 Val Ar g O31u Phe Leu G31y 420 <210O> 373 <21 1> 525 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (502) <223> RXS02937 <400> 373 gct accgcga agaact gt ac t agt t ct t cc at cagcaccc t cgaaacat c caacgcat tg gt gacaccgg t gt tgt cgcg gcagt tgt ct t tgcaagagt gt g at c agc aat Val I Ie Ser Asn g aa O31u gcg A a g at As p g at As p cat Hi s g aa O31u ccg Pr a g at As p aaa Ly s ccg Pr a cga Ar g ac c Thr cat Hi s g aa O31u ac g Thr aat As n ggt G31y 120 gt t ValI ggc G31y ggt G31y t ca Ser t gg Tr p gct A a t ct Ser g aa (31u gcg A a aaa Ly s caa O31n t ca Ser aac As n 75 ggt G31y cca Pr a gcg A a ct t Le u g aa O31u g aa O31u ct g Le u gga G31y gcg A a gcg A a ct g Le u ggc G31y gca A a ccg Pr a ggt G31y Le u at g Nibt gcg A a 110 gt a ValI g ac As p 15 gca A a gt g ValI gcg A a at g vb t at g vb t 95 cca Pr a gct A a at t I Ie ac c Thr gcg A a gt g ValI gcc A a gcg A a g at As p gct A a gcg A a gt g ValI ac c Thr gcg A a tt c Ph e c ag O31n c ag O31n gcg A a tt g Le u ac a Thr t gt cys tt c Ph e c ag O31n g ac As p cca Pr a 115 t gg Tr p ccc Pr a c ag O31n O31y aat As n cca Pr a aac As n 1 00 act Thr ct a Le u t aat tggct a at gaat cct t tt c <210O> 374 <211> 134 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 374 Page 492 seql i st cor r ec Val lie Ser Asn Gy Ou Gy Pro Val Val Ala 1 5 10 Asp Ala Leu Pro IVet Al a u Arg Ser Gy Lys 25 Sy Val Thr G n Val Asp Asn Thr Thr Gy G n 40 His Thr Cys G y His Asp Val His Ile Ser Ser 55 G n Ala Phe Asn Ser His Arg G u Leu Trp Asn 70 Val Phe G n Pro Ala G u G u Thr Ala Ala G y 90 Asp G n Asp Asn Ala Pro G y Asn His Ser Pro 100 105 IVet G n Pro Thr Leu Asp Arg G y Val G u Al a 115 120 Ser Ala Trp Leu Val Lys 130 <210> 375 <211> 966 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(943) <223> RXA02194 <400> 375 gaaatctccc agctcattta ttggacccag gtcatcatgg gaagatatct acaagaacct gtaggagttt taaagcaat c ted. txt Leu Arg G u Tyr G u Thr Leu Leu G y Thr Al a Ar g Al a Phe Leu Val 125 Sy Al a Pr o Sy Leu et Al a 110 Val Asp Al a Val Al a Met Met Pr o Al a gt c Val gaa G u gat Asp gcc Al a gac Asp ggc G y aac Asn ggc G y gca Al a t ac Tyr gct Al a ggt Sy ggc G y gca Al a aac Asn gct Al a gat Asp t cc Ser ct g Leu cgt Ar g gaa G u ggc G y cag G n ttc Phe t cc Ser gga G y ttc Phe 45 cag G n gct Al a cgt Ar g gag G u gat Asp ttc Phe ct c Leu gat Asp t ac Tyr cgc gcc Arg Ala 15 tcc aaa Ser Lys ttc ctt Phe Leu gat ttg Asp Leu gtc cac Val Hi s 80 gca gca Al a Al a 95 Page 493 ttgctcgcgg cctgaagcca atg ttg aaa atc gct IVet Leu Lys Ile Ala 1 at g aa at c ctc gcc IVet u Ile Leu Ala tcc ctc aac gtt ttt Ser Leu Asn Val Phe cgc cct aaa gat atc Arg Pro Lys Asp Ile ggt atc acc ggc cgc G y lie Thr G y Arg gaa gtt ctt tcc ctc G u Val Leu Ser Leu cca gct gat gaa gag Pro Ala Asp G u G u 100 seql i st cor r ect ed. t xt tgg agc atc gaa aag ctc gac ggc aag cgc atc gct acc tct tac ccc 451 Trp Ser Ile G u Lys Leu Asp y Lys Arg lie Ala Thr Ser Tyr Pro 105 110 115 aac ctt gtt cgc gat gac ctc gca gca cgt ggg ctt tcc gct gag gtg 499 Asn Leu Val Arg Asp Asp Leu Ala Ala Arg G y Leu Ser Ala G u Val 120 125 130 ctc cgc ctc gac ggt gca gta gag gta tcc atc aag ctt ggt gtc gca 547 Leu Arg Leu Asp Gy Al a Val G u Val Ser Ile Lys Leu G y Val Ala 135 140 145 gat gcc atc gcc gat gtt gta tcc acc ggc cgc acg ctg cgt cag caa 595 Asp Ala lie Ala Asp Val Val Ser Thr Gy Arg Thr Leu Arg n G n 150 155 160 165 ggt ctt gca cct ttc ggc gag gtt ctg tgc acc tct gag gct gtc att 643 Sy Leu Ala Pro Phe G y G u Val Leu Cys Thr Ser G u Ala Val lie 170 175 180 gtt ggc cgc aag gat gaa aag gtc acc cca gag cag cag atc ctg ctt 691 Val G y Arg Lys Asp G u Lys Val Thr Pro G u Gn On lie Leu Leu 185 190 195 cgc cgc atc cag gga att ttg cac gcg cag aac ttc ctc atg ctg gat 739 Arg Arg lie n G y lie Leu His Ala G n Asn Phe Leu IVet Leu Asp 200 205 210 tac aac gtc gac cgc gac aac ctg gac gct gcc act gca gta acc cca 787 Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala Thr Ala Val Thr Pro 215 220 225 ggc tta tcc ggc cca acg gta tcc cca ctg gca cgc gac aac tgg gtt 835 Sy Leu Ser y Pro Thr Val Ser Pro Leu Ala Arg Asp Asn Trp Val 230 235 240 245 gct gta cgc gcc atg gtg cca cgc agg tca gct aac gcc atc atg gat 883 Ala Val Arg Al a Iet Val Pro Arg Arg Ser Ala Asn Ala lle IVet Asp 250 255 260 aag ctt gct gga ctc ggc gct gaa gcc atc ctg gct tct gaa atc cgc 931 Lys Leu Ala y Leu Gy Ala u Ala lie Leu Ala Ser u Ile Arg 265 270 275 atc gcc cgc atc tagttttaac tacccccgaa aat 966 Ile Ala Arg Ile 280 <210> 376 <211> 281 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 376 IVet Leu Lys I e Ala Val Pro Asn Lys G y Ser Leu Ser G u Arg Al a 1 5 10 IVet Ou lie Leu Ala u Ala Gy Tyr Ala Gy Arg y Asp Ser Lys 25 Ser Leu Asn Val Phe Asp G u Ala Asn Asn Val G u Phe Phe Phe Leu 40 Arg Pro Lys Asp Ile Ala I e Tyr Val Ala Gy Gy n Leu Asp Leu 55 Page 494 seql i st cor r ect ed. t xt Gy I e Thr y Arg Asp Leu Ala Arg Asp Ser G n Al 70 75 a Asp Val His G u Pr o Al a Leu Lys 145 Thr Ser G n Phe Thr 225 Ar g Asn Al a Leu Asp Ser 115 Al a Gy Ar g Al a SIle 195 Met Val Asn SIle G u 275 Leu G u Pr o Val Al a G n 165 l le Leu Asp Pr o Val 245 Asp Ar g Gy Tr p Asn Leu Asp 150 Gy Val Ar g Tyr Gy 230 Al a Lys SIle Phe Ser Leu Ar g 135 Al a Leu Gy Ar g Asn 215 Leu Val Leu Al a Gy I e Val 120 Leu l Ie Al a Ar g SIle 200 Val Ser Ar g Al a Ar g 280 Ser Ser 90 G u Lys 105 Arg Asp Asp y Al a Asp Pro Phe 170 Lys Asp 185 G n y Asp Arg G y Pro Al a MVet 250 G y Leu 265 I e Thr Leu Asp Al a Val 155 Gy Su I e Asp Thr 235 Val Ar g Gy Al a 125 G u Ser Val Val Hi s 205 Leu Ser Ar g Tyr Lys 110 Al a Val Thr Leu Thr 190 Al a Asp Pr o Ar g Al a Ar g Ar g Ser Gy Cys 175 Pr o G n Al a Leu Ser 255 Al a SIe Gy I e Ar g 160 Thr G u Asn Al a Al a 240 Al a G y Al a G u Al a li e Leu 270 <210> 377 <211> 393 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(370) <223> RXA02195 <400> 377 tcgagccggc aggtgtcgca aaataaaacc aggtggggga gtgaaat t at tcgactaata tcctccccca aacacacatt gataactgtt gtgtggaaga atg tac cga gtg aag 115 IVet Tyr Arg Val Lys 1 aca ttt gac tcg ctg tac gaa gaa ctt ctt aac cgt gct cag acc cgc 163 Thr Phe Asp Ser Leu Tyr G u G u Leu Leu Asn Arg Ala G n Thr Arg 15 cct gaa ggg tct gga acc gtg gcc gcc ttg gat aaa ggc atc cat cat 211 Pro G u G y Ser G y Thr Val Ala Ala Leu Asp Lys G y lie His His 30 Page 495 ct a Leu gag G u t at Tyr at c SIle aag Lys gag G u acc Thr aag Lys gt c Val gat Asp gt c Val ct g Leu seql i st cor r ect ed. t xt atc gaa gaa gcc gga gag gtc tgg att gca gcc I le Gu u Al a Gy Gu Val Trp I e Ala Ala 45 gaa gag cta gcc gga gaa atc tcc cag ctc att G u G u Leu Al a G y G u Ile Ser G n Leu I e atc atg gtt gct cgc ggc ctg aag cca gaa gat Ile IVet Val Ala Arg y Leu Lys Pro G u Asp 75 80 taggagtttt aaagcaatca tgt <210> 378 <211> <212> PRT <213> Corynebacter i um gl ut ai cum <400> 378 IVet Tyr Arg Val Lys Thr Phe Asp Ser 1 5 Arg Ala G n Thr Arg Pro G u G y Ser 25 Lys G y lie Hi s Hi s Leu G y Lys Lys 40 Val Trp Ile Ala Ala G u Tyr G u Thr 55 Ile Ser n Leu Ile Tyr Trp Thr n 70 Leu Lys Pro G u Asp I e Tyr Lys Asn Leu Tyr G u G u Leu Leu Asn 10 Sy Thr Val Ala Ala Leu Asp Val lle u Gu Ala Gy u Asp G u G u Leu Al a y G u Val lie Mat Val Ala Arg Gy 75 Leu <210> 379 <211> 477 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(454) <223> RXA01097 <400> 379 gcgccaacgc agtgcttgcc gcgaccattt tccacttccg cgaagtaacc atcgccgaag taaagggagc cattaaagat gcaggatttg aggtgcggaa atg agt gac aat cca IVet Ser Asp Asn Pro 1 caa gag tat gag ctg gat tgg gac gtc gaa aag cga tta aag ctt aac Sn G u Tyr G u Leu Asp Trp Asp Val G u Lys Arg Leu Lys Leu Asn 15 gac gcc ggc ctg gtg ccg gca atc gtc cag gcc gac ggg acc aac gag Asp Ala y Leu Val Pro Ala I e Val n Ala Asp y Thr Asn u 30 gtc ctc atg atg gcc tgg atg gat acc cac gcg cta gcc tat act ttg Page 496 seql i st cor r ect ed. t xt Val Leu IVet IVet Ala Trp IVet Asp Thr His Ala Leu Ala Tyr Thr Leu 45 gcg acc cgc cgt gga acc tat ttt tct agg tcc cgc aac gag tac tgg 307 Ala Thr Arg Arg Gy Thr Tyr Phe Ser Arg Ser Arg Asn G u Tyr Trp 60 atc aag ggc ctg acc tct gga aac gtc caa gaa gtc acc gga ctt gcc 355 Ile Lys y Leu Thr Ser y Asn Val n G u Val Thr y Leu Ala 75 80 ctc gac tgc gac ggc gac acc gtc ctt ctg acc gtg aaa caa acc ggc 403 Leu Asp Cys Asp y Asp Thr Val Leu Leu Thr Val Lys G n Thr y 95 100 ggt gcg tgc cac act ggt gcc cac aca tgt ttc gac aat gac gtt ttg 451 G y Ala Cys His Thr G y Ala His Thr Cys Phe Asp Asn Asp Val Leu 105 110 115 ctg taaaagcaac aacgattaag gaa 477 Leu <210> 380 <211> 118 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 380 IVet Ser Asp Asn Pro n G u Tyr G u Leu Asp Trp Asp Val G u Lys 1 5 10 Arg Leu Lys Leu Asn Asp Ala y Leu Val Pro Ala lie Val G n Ala 25 Asp y Thr Asn G u Val Leu IVet IVet Ala Trp IVet Asp Thr His Ala 40 Leu Ala Tyr Thr Leu Al a Thr Arg Arg y Thr Tyr Phe Ser Arg Ser 55 Arg Asn u Tyr Trp Ile Lys y Leu Thr Ser y Asn Val Gn u 70 75 Val Thr y Leu Ala Leu Asp Cys Asp y Asp Thr Val Leu Leu Thr 90 Val Lys G n Thr G y G y Ala Cys His Thr G y Ala His Thr Cys Phe 100 105 110 Asp Asn Asp Val Leu Leu 115 <210> 381 <211> 861 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(838) <223> RXA01100 <400> 381 gctgtgggct actcaattcc acccagaaaa atcaggtgac gcaggcgcac agctactgcg Page 497 seql i st cor r ect ed. t xt aaactggatc aactacatct aacagatagg atcaatattc ctt cct Leu Pro ggc gag G y G u ctg aag Leu Lys gac gca Asp Al a gtc ggc Val G y gat gag Asp u att ggt I e G y caa cgc G n Ar g gat ggt Asp y 135 gat ctg Asp Leu 150 ttc gtg Phe Val gtt gag Val G u gca t ct Al a Ser tac cag Tyr G n 215 gag cac G u Hi s 230 gca Al a gcc Al a tgg Tr p gcg Al a aag Lys tct Ser acc Thr t at Tyr 120 gaa G u tgg Tr p gtt Val ct g Leu ggt Gy 200 gat Asp aag Lys gat Asp act Thr gag G u aac Asn gat Asp gag Gu gct Al a gag G u cgc Ar g gtt Val gat Asp 170 cgt Ar g at t SIle ggc Gy acc gt a Val gaa G u cag G n cgt Ar g gtt Val 75 cgc Ar g ct g Leu aag Lys acc Thr ct c Leu 155 gt g Val gag G u tct Ser at t SIle ct c gtt Val aag Lys ggt Gy ggt Gy 60 gat Asp gcg Al a gag G u at t SIle cgt Ar g 140 gag G u t cc Ser gtt Val gtt Val gat Asp 220 gaa G u aac Asn tct Ser gca Al a 45 t cc Ser gt g Val ct g Leu aag Lys gct Al a 125 gga Gy cgt Ar g aag Lys gct Al a ttg Leu 205 t cc Ser gag G u gga Gy t at Tyr aag Lys aac Asn gag G u gca Al a cca Pr o 110 gt c Val aac Asn ttg Leu gac Asp gca Al a 190 gaa G u gt c Val gct Al a caa G n 15 ggc Gy tgg Tr p cat Hi s ct c Leu acc Thr 95 gag G u gat Asp ggt Gy gat Asp ggc Sy 175 gct Al a gat Asp at c SIle ttg Leu gca Al a acc Thr ttg Leu gag G u act Thr 80 ggt Gy tgg Tr p at c SIle tgg Tr p t cc Ser 160 acc Thr aca Thr gtt Val at t SIle gct Al a 240 at g let 1 gtt Val cct Pr o cac Hi s at g let ggc Gy gca Al a at t SIle gct Al a gt c Val 145 caa G n ttg Leu gac Asp ttg Leu ggc Gy 225 gca Al a cgc Ar g ttg Leu ttt Phe at g let ggt Gy cgt Ar g gct Al a gt g Val 130 t cc Ser ggt Gy agt Ser gca Al a gaa G u 210 aag Lys gt a Val acc ttc act att Thr Phe Thr Il e ct a Leu gaa G u gt g Val gcg Al a at c SIle cgt Ar g tct Ser 115 cgt Ar g gat Asp tgt Cys ggt Gy cct Pr o 195 ct a Leu gca Al a gaa G u gat Asp t cc Ser gac Asp gaa G u cgt Ar g gt a Val 100 gcg Al a ttg Leu ggt Gy gca Al a cca Pr o 180 at c SIle gcc Al a ctt Leu aag Lys cag G n gca Al a ctg Leu att SIe gat Asp aac Asn att SIe gaa G u ggc Gy cgt Ar g 165 aat Asn gtg Val aag Lys t at Tyr ct c Leu 245 Phe Thr Leu 235 ggt Gy taatacatgg atgctcgtgg gat Page 498 seqI i st car r ect ed. t xt <210O> 382 <21 1> 246 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri curn <400> 382 I'bt Thr Phe Thr Ilie Leu Pro Ala Val ValI Pr o Hi s vb t G31y Al a I Ie Al a ValI 145 O31n Le u As p Le u O31y 225 Al a Le u O31u ValI Al a I Ie Ar g Ser 115 Ar g As p cys O31y Pr o 1 95 Le u Al a O31u As p (1n (1y Ser Al a Leu Asp Leu Asp O3 u Il e Val 70 Arg Asp Asp Val Asn Ilie 1 00 Al a IlIe 03 n Leu 03 u Asp (31y 1y Asp 150 Al a Ar g Phe 1 65 Pr o Asn Val 1 80 Ilie Val Al a Al a Lys Tyr Leu Tyr 03 u 230 Lys Leu 03 y 245 O31u Ly s Al a G31y O31u G y Ar g G31y 135 Le u ValI O31u Ser O31n 215 Hi s Al a Tr p 40 Al a Ly s Ser Thr Tyr 120 O31u Tr p ValI Le u G31y 200 As p G31y O31n Ph e Le u Le u Al a 1 G31y Tr p (31u Thr Le u 1 G31y (31u As p 10 Thr (31u As n As p (31u 90 Al a (31u Ar g ValI As p 1 70 Ar g I Ie G31y ValI (31u O31n Ar g ValI Ar g Le u Ly s Thr Le u 1 ValI O31u Ser I Ie As n Ser Al a Ser ValI Le u Ly s Al a 125 O31y Ar g Ly s Al a Le u 205 Ser O31n O31y Tr p Hi s Le u Thr G u As p O31y As p O31y 1 Al a As p I Ie Lys Phe Thr Leu 03 u 03 u Al a Leu Al a 235 240 <210O> 383 <21 1> 756 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (733) <223> RXA01101 <400> 383 at cgcagct a ggccagt gt g gt ggat at aa aaccct t t t g gggagaaaga aact cgact g Page 499 seql i st cor r ect ed. t xt cggttcttga tcctgaaagc acgtgacata aactatcggc atg acc aaa act gtc 115 Met Thr Lys Thr Val 1 gcc ctt ctc gac tac gga tct gga aac ctt cgt tct gct caa cgc gca 163 Ala Leu Leu Asp Tyr G y Ser y Asn Leu Arg Ser Ala G n Arg Ala 15 cta gag cgt gcc ggt gca gaa gtt atc gtg agc tcc gat cca gaa gtt 211 Leu G u Arg Ala G y Al a G u Val Ile Val Ser Ser Asp Pro G u Val 30 tgc acc aac gct gat ggc ctc cta gtt cct gga gtg ggc gca ttt gat 259 Cys Thr Asn Ala Asp G y Leu Leu Val Pro y Val G y Ala Phe Asp 45 gcc tgc atg aag ggt ttg aaa aac gtc ttc gga cat cgc att atc gga 307 Ala Cys IVet Lys Gy Leu Lys Asn Val Phe Gy His Arg lie lie G y 60 cag cgt ctt gct ggt gga cgt cca gtg atg ggt att tgt gtg ggc atg 355 G n Ar g Leu Ala G y G y Arg Pro Val IVet Gy le Cys Val Gy Iet 75 80 cag atc ctg ttc gat gaa ggc gat gag cac ggc att aag tca gct ggt 403 G n I e Leu Phe Asp G u G y Asp G u Hi s G y lie Lys Ser Al a G y 95 100 tgc ggc gag tgg cct ggc aaa gtg gaa cgc ctc caa gcg gag atc ctg 451 Cys Gy u Trp Pro y Lys Val u Arg Leu n Al a u Ile Leu 105 110 115 cct cac atg ggg tgg aac aca ctt gaa atg cct acc aac tca cca atg 499 Pro His Iet G y Trp Asn Thr Leu G u IVet Pro Thr Asn Ser Pro IVet 120 125 130 ttt gag gga att tca cct gat gag cgt ttc tac ttc gtg cac tcc tat 547 Phe G u Gy lie Ser Pro Asp G u Arg Phe Tyr Phe Val His Ser Tyr 135 140 145 ggt gtg cgc aag tgg acg ttg gaa acc gac gat ctg acc acg cct cca 595 Gy Val Arg Lys Trp Thr Leu G u Thr Asp Asp Leu Thr Thr Pro Pro 150 155 160 165 gag gtt gtg tgg gcg aag cac gaa aat gat cgt ttt gtg gca gct gtg 643 G u Val Val Trp Ala Lys His G u Asn Asp Arg Phe Val Ala Ala Val 170 175 180 gaa aac ggc acg ctg tgg gct act caa ttc cac cca gaa aaa tca ggt 691 G u Asn Gy Thr Leu Trp Ala Thr G n Phe His Pro G u Lys Ser y 185 190 195 gac gca ggc gca cag cta ctg cga aac tgg atc aac tac atc 733 Asp Ala Gy Ala n Leu Leu Arg Asn Trp lie Asn Tyr Ile 200 205 210 taacagatag gatcaatatt cat 756 <210> 384 <211> 211 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 384 Iet Thr Lys Thr Val Al a Leu Leu Asp Tyr G y Ser G y Asn Leu Arg 1 5 10 Page 500 seqi i st car r ect ed. t xt Se r Ala O31n Ar g Ala Leu Ou Arg Ala G1y Ala Olu Val lieVal Ser 25 Ser ValI Hi s li1e I Ie O31n Thr Ph e 145 Le u Ph e Pr o As n Pr o Al a I Ie ValI Ser O31u 115 Ser Hi s Thr Al a Ly s 1 95 li1e ValI As p G31y vb t G31y Le u vb t Tyr Pr o 1 65 ValI G31y Cy s Al a O31n 70 O31n Cy s Pr o Ph e (31y 150 O31u O31u As p Thr cys Ar g I Ie O31y Hi s O31u 135 ValI ValI As n Al a Al a Ly s Al a Ph e Tr p 1 Oy le Ly s Tr p Thr 1 Al a As p O31y O31y As p 90 Pr a Tr p Ser Tr p Al a 1 70 Le u O31n O31y Le u O31y O31u O31y As n Pr a Thr 1 Ly s Tr p Le u Le u Ly s Ar g O31y Ly s Thr As p 140 Le u Hi s Al a Le u Le u As n Pr a As p ValI Le u 125 O31u (31u O31u Thr Ar g 205 Pr a Ph e vb t Hi s Ar g vb t Ph e As p As p 1 Ph e Tr p <210O> 385 <21 1> 723 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (700) <223> RXN01657 <400> 385 cct ccgt cat t gccgacgt a t cccgcggcc t gggt gaagc cat ggt gggc at caacgt at ccgacgt tcc agcaccacac cgact cgccg agcgcggct g gt g at c gt t gga gt t Val I Ie Val O31y Val 1 t t agct ct ccag ggc ggg gtg gaa gaa cac ct cacc gcct t ggaa gct Leu Ala Leu (1n (1y (1y Val1(3 Ou 1u Hi s Leu Thr Ala Leu (1u Al a 15 ct c gga gcg acg acc cga aaa gt a cgt gt g cca aag gac ct t gat ggt Leu O31y Al a Thr Thr Arg Lys Val Ar g Val Pr o Lys Asp Leu Asp O31y 30 ctc gaa ggc atc gtc atc ccc ggc ggg gaa tcc acc gtg ttg gac aaa Leu 31u (31y I Ie Val I Ie Pro (31y 31y (31u Ser Thr Val Leu Asp Lys Page 501 seql i st cor r ect ed. t xt 45 ctg gct cgg aca ttc gac gtg gta gaa cct cta gcg aat ctc att cgc 307 Leu Ala Arg Thr Phe Asp Val Val G u Pro Leu Ala Asn Leu lie Arg 60 gac ggc cta ccc gtt ttc gct acc tgc gct ggc ctg atc tat ctg gcg 355 Asp y Leu Pro Val Phe Ala Thr Cys Ala y Leu lie Tyr Leu Ala 75 80 aaa cac ctc gac aac cca gca agg gga caa caa acc ttg gcg gta gtg 403 Lys His Leu Asp Asn Pro Ala Arg G y G n n Thr Leu Ala Val Val 95 100 gac gtg gtg gtg cgt cga aac gca ttt ggc gcc caa cgc gaa tcc ttc 451 Asp Val Val Val Arg Arg Asn Ala Phe Gy Ala G n Arg u Ser Phe 105 110 115 gac acc acc gtg gat gtt tcc ttc gac ggt gca aca ttc ccc gga gtg 499 Asp Thr Thr Val Asp Val Ser Phe Asp Gy Ala Thr Phe Pro y Val 120 125 130 cag gcc tcg ttt atc cga gct ccc atc gtc act gct ttt ggt cct acg 547 G n Ala Ser Phe Ile Arg Ala Pro Ile Val Thr Ala Phe y Pro Thr 135 140 145 gta gaa gcg atc gct gct ctc aac ggt ggg gag gtg gtt ggt gta cgc 595 Val u Ala Ile Ala Ala Leu Asn G y y u Val Val y Val Arg 150 155 160 165 caa ggc aac atc atc gcg ctg tct ttc cat ccc gaa gaa acc ggc gat 643 Sn G y Asn lie ile Ala Leu Ser Phe His Pro G u G u Thr G y Asp 170 175 180 tac cgc atc cac caa gcc tgg ctg gac ctg gtg aga aaa cac gct gaa 691 Tyr Arg Ile His G n Al a Trp Leu Asp Leu Val Arg Lys His Ala G u 185 190 195 ctg gcg att tgatgttttc ggtagcgctc tgt 723 Leu Al a l e 200 <210> 386 <211> 200 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 386 Val lie Val y Val Leu Ala Leu G n y y Val G u G u His Leu 1 5 10 Thr Ala Leu G u Ala Leu G y Ala Thr Thr Arg Lys Val Arg Val Pro 25 Lys Asp Leu Asp y Leu G u y Ile Val Ile Pro G y Gy Gu Ser 40 Thr Val Leu Asp Lys Leu Ala Arg Thr Phe Asp Val Val G u Pro Leu 55 Ala Asn Leu lie Arg Asp y Leu Pro Val Phe Ala Thr Cys Ala y 70 75 Leu IIe Tyr Leu Ala Lys His Leu Asp Asn Pro Ala Arg G y n O n 90 Thr Leu Ala Val Val Asp Val Val Val Arg Arg Asn Ala Phe y Ala Page 502 O31n Thr A a 145 ValI (31u Ar g (31u 115 Pr o G31y G31y Thr Hi s 1 95 Ph e ValI Thr Ar g 1 65 As p (31u Thr A a 135 O31u G31y Ar g A a seqi i st car r ect ed. t xt 1 Thr Val Asp Val Ser Phe 120 125 Ser Phe I Ie Ar g Ala Pr o 140 A a I I e A a A a Leu As n 1 55 As n I I e I I e A a Leu Ser 1 70 li1e Hi s O31n Ala Tr p Leu 1 le 200 O3 y A a Val Thr O31y (1u 1 Hi s Pr o 1 Leu Val <210O> 387 <21 1> 601 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (601) <223> FRXA01657 <400> 387 cct ccgt cat t gccgacgt a t cccgcggcc t gggt gaagc ccgacgttcc agcaccacac cgactcgccg agcgcggctg cat ggt gggc at caacgt at tt a Le u ct c Le u ct c Le u ct g Le u g ac As p aaa g ac As p g ac As p ct c Le u gcg A a ggc G31y cgg Ar g ct a Le u ct c Le u gt g ValI ac c Th r ggc G31y ac c Th r gt c ValI tt c Ph e gt t ValI aac As n cgt Ar g g at As p ggg G31y cga Ar g at c I Ie gac As p t t c Ph e 75 cca Pr o cga Ar g gt t ValI gt g ValI aaa Lys5 ccc Pr o gt g ValI gct A a gca A a aac As n t cc Ser g aa O31u gt a ValI G31y 45 gt a ValI ac c Th r ag g Ar g gca A a tt c Ph e gaa cac ct c O3 u Hi s Leu 15 cgt gt g cca Ar g Val Pr o ggg gaa t cc O3 y 03 u Ser gaa cct ct a O31u Pr o Leu t gc gct ggc Cys A a 03 y gga caa caa O31y (1n (1n 95 ttt ggc gcc Phe 03 y A a 110 gac ggt gca Asp 03 y A a gt g ValI 1 ac c Thr aag Lys5 ac c Thr gcg A a ct g Le u ac c Thr c aa O31n gcc A a g ac As p gt g ValI aat As n at c I Ie tt g Le u cgc Ar g at c gt t gga gt t lieVal Gy Val- t tg Le u ct t Le u tt g Le u ct c Le u t at Tyr gcg A a g aa O31u 115 g aa O31u g at As p g ac As p at t I Ie ct g Le u gt a ValI 1 00 t cc Ser aca t tc ccc Thr Phe Pr o gga gt g 03 y ValI Page 503 seql i st cor r ect ed. t xt 120 125 130 cag gcc tcg ttt atc cga gct ccc atc gtc act gct ttt ggt cct acg G n Ala Ser Phe Ile Arg Ala Pro Ile Val Thr Ala Phe y Pro Thr 135 140 145 gta gaa gcg atc gct gct ctc aac ggt ggg gag gtg gtt ggt gta cgc Val u Al a I e Ala Ala Leu Asn G y G y u Val Val y Val Arg 150 155 160 165 caa ggc G n Gy <210> 388 <211> 167 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 388 Val Ile Val Sy Val Leu Ala Leu G n Sy Val u u His Leu 1 Thr Lys Thr Al a Leu Thr G n Thr Al a 145 Val Leu Leu Leu Leu Tyr Al a G u 115 Pr o Gy Gy 5 Al a Gy Lys Ar g Al a Val Phe Val Thr Ar g 165 Gy G u Al a Gy Hi s Val Thr Al a 135 G u Gy Al a Gy 40 Ar g Leu Leu Val Thr 120 Ser Al a Thr I e Thr Pr o Asp Val 105 Val Phe l Ie Ar g SIle Asp Phe Pr o Ar g Val Ar g Al a 155 Lys Pr o Val Al a Al a Asn Ser Al a 140 Leu Val Gy Val Thr Ar g Al a Phe 125 Pr o Asn Val G u Pr o Al a G n Gy Gy Val Gy <210> 389 <211> 897 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(874) <223> RXA01098 <400> 389 aaaagggctc acgatgtgct gttaagtaag attgaaaaag ttcggttgat gcatgcagat Page 504 seql i st cor r ect ed. t xt gcaggtaatg accagtcgtt aaatgaggag tacaagtaaa atg ggc gtg gca att Met Gy Val Ala Ile cga Ar g gt g Val aag Lys acc Thr acc Thr agc Ser tcg Ser t cc Ser cgc Ar g acc Thr 150 aag Lys gac Asp gtt Val aag Lys ctt Leu 230 aag Lys at t SIle ttt Phe t at Tyr tcg Ser gat Asp gaa Gu aac Asn cgt Ar g 120 cct Pr o ggc Gy ggc Gy gac Asp gca Al a 200 gag G u gcg Al a gcc Al a tgc Cys aac Asn gag G u cat Hi s gt g Val gtt Val tct Ser ggt Gy ggt Gy t cc Ser gag G u 170 acc Thr gt a Val ttc Phe at t l Ie aaa Lys 250 ct g Leu ct c Leu gaa G u ggt Gy tt c Phe 75 gat Asp gcg Al a gct Al a gga Gy aag Lys 155 ct g Leu aaa Lys t cc Ser cca Pr o tt c Phe 235 gat Asp gac Asp cgc Ar g ggg Gy cgc Ar g at c SIle caa G n at t l Ie cag G n act Thr 140 t cc Ser ggc Gy aac Asn at t SIle cca Pr o 220 cac Hi s gca Al a gt g Val gat Asp gca Al a 45 ggc Gy cct Pr o ttg Leu gcc Al a tgc Cys 125 cct Pr o gca Al a gtt Val ggc Gy cct Pr o 205 gct Al a ttc Phe gga Gy gac Asp gct Al a gat Asp acc Thr ct g Leu ct g Leu cgt Ar g 110 at c SIle cag G n gaa G u ggc Sy ttt Phe 190 gt a Val gtt Val cgc Ar g ttt Phe aac Asn 15 ggc Gy gag G u at g Met act Thr cgc Ar g 95 cca Pr o gt g Val cca Pr o ctt Leu gaa G u 175 gac Asp at c SIle gca Al a gaa G u gag G5u 255 ggc cgg gtt G y Ar g Val gat cct gtg Asp Pro Val ctg acc ttc Leu Thr Phe ctg gat gtt Leu Asp Val gtc ggt ggc Val Gy y 80 gct ggc gcc Al a G y Al a gaa ct g ct g G u Leu Leu ttg tct gtg Leu Ser Val 130 tct ggt ttt Ser G y Phe 145 gat gca at c Asp Al a lile 160 att ctg ctc Ile Leu Leu ct a gag ct g Leu G u Leu gcc tcc ggc Al a Ser G y 210 gct ggc gcc Al a G y Al a 225 gt a acc at c Val Thr Ile 240 gtg cgg aaa Val Arg Lys gtt Val gag G u ct g Leu gtt Val ggc Gy gac Asp t ca Ser 115 gat Asp gaa G u gag G u aac Asn ct g Leu 195 ggc Gy aac Asn gcc Al a aaa Lys ttg Leu gat Asp cga Ar g gt g Val aag Lys 100 gag G u gcc Al a gt c Val tgg Tr p t cc Ser 180 gaa G u gct Al a gca Al a gaa G u ggc Gy gca Al a gtc Val cgc Ar g cgc Ar g gtt Val ctg Leu agg Ar g acc Thr gca Al a 165 at g vbt aaa Lys ggc Gy gtg Val gta Val 245 t gagt gacaa Page 505 t ccacaaag t atseqI i st car r ect ed. t xt89 <210O> 390 <21 1> 258 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 390 b t (1y ValIAlaIHeArg Val Ile Pro Cys Leu AspVal AspAsn Oy 1 5 10 Ar g Val Val Lys G31y Val Asn Phe O31u Asn Leu Arg Asp Ala Gy Asp 25 Pr oVal O31u Leu AiaLys Ar gTyr As p (1u (1u 1y Al aAs p (1u Le u 40 Thr Phe Leu As p Val Thr Al a Se r Lys Hi s G31y Ar g G31y Th r I'b t Leu 55 As p Val Val Ar g Ar g Thr Al a As p O31n Val Phe Ilie Pro Leu Thr Val 70 75 G31y (1y 1y Val Ar gSer (31u 1u Asp Val As p (1n Leu Leu Arg Ala 90 O31y Al a As p Lys Val Ser Val As n Thr Ser Al a I Ie Al a Arg Pro O31u 100 105 110 Leu Leu Ser O31u Leu Ser Lys Ar g Phe O31y Al a O31n Cys I Ie Val Leu 115 120 125 Ser Val As p Al a Ar g Ar g Val Pr o O31u 31y G31y Thr Pr o O31n Pro Ser 130 135 140 G31y Phe O31u Val Thr Thr Hi s 31y G31y Ser Lys Ser Al a O31u Leu As p 145 150 155 160 Al aIIe (1u Tr pAl aLys Ar g (1y (1u (1u Leu (1y Val O31y (1u I Ie 165 170 175 Leu Leu As n Ser N/b t As p G31y As p G31y Thr Lys As n G31y Phe As p Leu 180 185 190 O31u Leu Leu O31u Lys Val Ar g Al a Al a Val Ser I Ie Pr o Val I Ie Al a 195 200 205 Ser G31y G31y Al a G31y Lys Al a O31u Hi s Phe Pr o Pr o Al a Val Al a Al a 210 215 220 G31y Al a As n Al a Val Leu Al a Al a Thr I Ie Phe Hi s Phe Ar g O31u Val 225 230 235 240 Thr Ilie Al a O31u Val Lys G31y Al a Ilie Lys Asp Al a G31y Phe O31u Val 245 250 255 Arg Lys <210> 391 <211> 729 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221> CIDS Page 506 seqi i st car r ect ed. t xt <222> (706) <223> RXN01104 <400> 391 cacttgcgca ctaccattgg tgtgcctgag gaaaatgatg gagatcatca agctgaacct gtaagagaga agaatttttc cgtttttgga cgcagctgca at g act l'bt Thr gt c gca cca Val Ala Pro ag a Ar g gag (31u cca Pr o g at As p ac c Thr at t I Ie at g vb t t ac Tyr ggt G31y gcg A a 1 50 g ac As p ct g Le u act Thr ggt G31y aac As n tt c Ph e aaa Ly s g aa (31u g ac As p gag (31u gt g ValI 120 c ac Hi s aac As n c ac Hi s ggt G31y gga G31y 200 gca A a g ac As p c ac Hi s cat Hi s ac c Thr aaa Ly s tt a Le u t cc Ser gca A a cga Ar g at c I Ie 1 70 gt a ValI acc Thr ggc G31y at g vb t gcc A a gcc A a 75 ggc G31y gt g ValI G31y acc Thr at c I Ie 155 acc Thr gag (31u cgc Ar g ac c Thr ct c Le u aag Ly s at c I Ie at c I Ie gag (31u g aa (31u gt g ValI 140 ac c Thr g aa (31u at g vb t ac c Thr ggc G31y act Thr 45 ggc G31y gt g ValI cgc Ar g t cc Ser cca Pr o 125 at c I Ie ct c Le u gca A a g at As p ac c Thr aaa Ly s gca A a g ac As p ct c Le u cgt Ar g gt g ValI 110 g ac As p aac As n c ac Hi s gag (31u cct Pr o 1 90 ag c Ser 15 gt a ValI tt c Ph e at c G31y tt c Ph e 95 gt g ValI c ac Hi s gag (31u gt g ValI t ac Tyr 1 75 cgt Ar g g aa (31u g at As p ggc G31y gag (31u c aa O31n gca A a g at As p at g vb t c ac Hi s at c I Ie 1 aag Ly s c aa O31n 1 t cc Ser at c I Ie gt g ValI at c I Ie gca A a t cc Ser at c I Ie at c I Ie tt c Ph e 145 t gc cys gct A a ac a Thr g ac As p g at As p c ac Hi s g ac As p ct c Le u t gc cys t cc Ser ac c Thr 130 tt t Ph e c ac Hi s gt t ValI gga G31y at c I Ie ac c Thr ggc G31y gca A a ct t Le u c ag O31n ggt G31y 115 t cc Ser g aa O31u t ac Tyr gcc A a at c I Ie 1 ac c Thr ggc G31y agt Ser c ac Hi s g ac As p ct g Le u 1 00 cgc Ar g gt g ValI ac c Thr ggc G31y cgt Ar g 1 cca Pr o gcg ct c t agacat gaa ct ct t ct ccc at c A a Leu <210O> 392 <21 1> 202 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn Page 507 seql i st cor r ect ed. t xt <400> 392 Met 1 Ser SIle Val SIle Al a Ser SIle SIle Phe 145 Cys Al a Thr Thr Val Al a SIle Thr Gy Al a Leu G n Gy 115 Ser G u Tyr Al a SIle 195 Pr o 5 Val Leu Phe Hi s Al a Pr o Pr o SIle Leu Ar g 165 Al a Ser Arg lie y Thr Ala Thr Arg Thr Thr Ser u 10 SIle Phe Leu Val Gy Asp Phe Gy 135 Leu Pr o Ar g Lys Leu Asp Val Asp Lys Al a 105 I le Tyr Ser Hi s Al a 185 Al a Asp Hi s Hi s Thr Lys 90 Leu Ser Al a Ar g SIle 170 Val Leu Gy bet Al a Al a Gy Val Gy Thr SIle 155 Thr G u Gy Thr Gy Val Ar g Ser Pr o 125 I le Leu Al a Asp Lys Al a Asp Leu Ar g Val 110 Asp Asn Hi s G u Pr o 190 Val Phe SIe Gy Phe Val Hi s G u Val Tyr 175 Ar g <210> 393 <211> 729 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(706) <223> FRXA01104 <400> 393 cacttgcgca ctaccattgg tgtgcctgag gaaaatgatg cgtttttgga cgcagctgca gagatcatca agctgaacct gtaagagaga agaatttttc atg act gtc gca cca Met Thr Val Ala Pro 1 aga att ggt acc gca acc cgc acc acc agc gaa tcc gac atc acc gtc Arg lie y Thr Ala Thr Arg Thr Thr Ser u Ser Asp lie Thr Val 15 gag atc aac ctg gac ggc acc ggc aaa gta gat atc gat acc ggc ctg G u Ile Asn Leu Asp G y Thr y Lys Val Asp Ile Asp Thr y Leu 30 cca ttt ttc gac cac atg ctc act gca ttc ggc gtg cac ggc agt ttt Pro Phe Phe Asp His IVet Leu Thr Ala Phe G y Val His G y Ser Phe Page 508 seql i st cor r ect ed. t xt gat ctg aaa gtc cat gcc Asp Leu Lys Val His Ala acc gtg gaa gat acc gcc Thr Val G u Asp Thr Al a 75 att ggc gac aag aaa ggc I e G y Asp Lys Lys G y atg gat gag gca tta gtg IVet Asp G u Ala Leu Val 105 tac ttc gtg atc tcc ggc Tyr Phe Val lie Ser G y 120 ggt gga cac tac gca acc G y G y Hi s Tyr Al a Thr 135 gcg ctc aac tcc cga atc Ala Leu Asn Ser Arg Ile 150 155 gac cct cac cac atc acc Asp Pro His His Ile Thr 170 ctg cgc ggt gcc gta gag Leu Arg y Ala Val G u 185 act aag gga gcg ctc taga Thr Lys Gy Ala Leu 200 <210> 394 <211> 202 <212> PRT <213> Corynebacter i um gl <400> 394 IVet Thr Val Ala Pro Arg 1 5 Ser Asp lie Thr Val G u Ile Asp Thr y Leu Pro Val His G y Ser Phe Asp Ile Asp Ala His His Thr 70 Ala Leu Leu Asp Ala Il e Ser Cys G n Leu Pro ?bet aag Lys 60 at c l Ie at c SIle gag G u gaa G u gt g Val 140 acc Thr gaa G u at g Met ggc G y gt g Val cgc Ar g t cc Ser cca Pr o 125 at c SIle ct c Leu gca Al a gat Asp gac Asp ct c Leu cgt Ar g gt g Val 110 gac Asp aac Asn cac Hi s gag G u cct Pr o 190 atc gag at c Ilie u ll e ggc caa gca G y G n Al a 80 ttc gca tcc Phe Al a Ser 95 gtg gat at c Val Asp Ile cac at g at c His Met Ile gag cac ttc G u Hi s Phe 145 gtg atc tgc Val Ile Cys 160 tac aag gct Tyr Lys Al a 175 cgt caa aca Arg G n Thr gac Asp ct c Leu tgc Cys t cc Ser acc Thr 130 ttt Phe cac Hi s gtt Val gga G y gca Al a ctt Leu cag G n ggt Sy 115 t cc Ser gaa G u t ac Tyr gcc Al a at c SIle 195 cac Hi s gac Asp ct g Leu 100 cgc Ar g gt g Val acc Thr ggc G y cgt Ar g 180 cca Pr o cac Hi s gct Al a ccc Pr o cca Pr o at c SIe ct c Leu cgc Ar g 165 gcg Al a t cc Ser .catgaa ctcttctccc atc ut ari cum li e G y Thr Ile Asn Leu 25 Phe Phe Asp 40 Leu Lys Val Val G u Asp G y Asp Lys Asp G u Al a Al a Thr 10 Asp G y His ebt I Hi s Ala I Thr Al a Lys G y 90 Leu Val Page 509 Thr Sy Thr Sy Val Ar g Ser Thr Lys Al a Asp Leu Ar g Val Ser Val Phe SIe Sy Phe Val I e I e Phe 145 Cys Al a Thr Gy 115 Ser G u Tyr Al a SIle 195 Pr o SIle Leu Ar g 165 Al a Ser Tyr Gy Al a 150 Asp Leu Thr Phe Gy 135 Leu Pr o Ar g Lys seql i st cor r ect ed. t xt 105 Val lie Ser Gy Ou Pro 120 125 His Tyr Ala Thr Val I e 140 Asn Ser Arg lie Thr Leu 155 His His I e Thr G u Ala 170 Gy Ala Val u Met Asp 185 G y Al a Leu 200 Hi s Met G u Hi s Val lie 160 Tyr Lys 175 Arg G n <210> 395 <211> 987 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(964) <223> RXN00446 <400> 395 t gct acgaag tt at ct agt a at gaagtt ag ttttt cccct ggtgacggag gctacttggg gggctaatcg gtacccggat ctcccggcag cagttgatgc ct c Leu acg Thr acg Thr gct Al a ccg Pr o at c SIle tcg Ser gtt Val gag G u ggt Gy gct Al a cca Pr o act Thr gat Asp acc Thr aac Asn ctt Leu ggc Gy ggc Gy ttc Phe gat Asp acc Thr at c SIle gt c Val gca Al a tcg Ser gat Asp gcg Al a cag G n 75 cgc Ar g acc Thr gtt Val gag G u tct Ser gag G u cag G n aat Asn ct c Leu cag G n gtt Val cat Hi s gcg Al a gt c Val 45 gt c Val cat Hi s at t SIle gcg Al a ggg G y tta Leu ct g Leu at t SIle gcg Al a gat Asp ttc Phe cag G n 110 ct g Leu gtt Val caa G n cca Pr o gcc Al a gat Asp 80 tgc Cys gat Asp atg ggt Vbet y 1 gag ttt G u Phe cag ctg G n Leu tgg cgc Trp Arg act cct Thr Pro gcg atg Al a Vbet aac ccc Asn Pro aat ttc Asn Phe gcg gtt gag Ala Val u gac cag gtc Asp G n Val gtt cag gca Val n Ala agc ttt gag Ser Phe G u gtt gcc att Val Ala lle gca gcc gcg Al a Al a Al a aac aat cct Asn Asn Pro 100 atg gaa aag IVet G u Lys 115 gat gag gct tat ttt gag ttc Asp G u Ala Tyr Phe G u Phe Page 510 seql i st cor r ect ed. t xt aac cgc gcg gac gac acc Asn Arg Ala Asp Asp Thr 135 gac aac gtg att ggt ttg Asp Asn Val Ile Gy Leu 150 155 ggc ttg cgt gtt ggt tac Sy Leu Arg Val y Tyr 170 atg aat aag gtg gct att IVet Asn Lys Val Ala Ile 185 gca gcg ctt gcg agt ttg Ala Ala Leu Ala Ser Leu 200 gag gaa acc gtc gaa aag Su u Thr Val u Lys 215 gcg ccg acg cag gcc aat Ala Pro Thr G n Ala Asn 230 235 gag ttg gcg gct aaa ttg G u Leu Ala Ala Lys Leu 250 ccc gag ggt gcg cgc att Pro u y Ala Arg lie 265 ctg ctg cgc gcg tgg gag Leu Leu Arg Ala Trp G u 280 tgc <210> 396 <211> 288 <212> PRT <213> Corynebacter i um gl <400> 396 Met y Ala Val G u Leu 1 5 G u Phe Asp G n Val Thr G n Leu Val G n Ala Thr Trp Arg Ser Phe G u Al a Thr Pro Val Ala lie Pro 70 Ala Iet Ala Ala Ala Ile cca Pr o 140 cgc Ar g gcc Al a cct Pr o aat Asn cgt Ar g 220 ttc Phe gcc Al a tcg Ser gcc Al a ttc Phe gga Sy gcg Al a 190 gcc Al a gct Al a tgg Tr p cac Hi s acc Thr 270 gag G u aag Lys 160 gca Al a aat Asn gag G u gt g Val ccg Pr o 240 at c SIle gcc Al a gaa G u 145 gcg Al a gag G u t ca Ser ttg Leu t ca Ser 225 ggc G y gt g Val gag G u at c SIle t at Tyr at c SIle gca Al a at g Met 210 gcg Al a gag G u at t SIle gaa G u cac cgc cac His Arg His ggc ctg gcg G y Leu Al a 165 atc gca gcg I e Al a Al a 180 gct cag gcg Ala G n Ala 195 gaa cgg gtg Su Arg Val ctt ggt gct Leu G y Al a ggc gcc gct G y Al a Al a 245 cgc gcg ttc Arg Ala Phe 260 act gac aag Thr Asp Lys gcc atc aat gct ggg Ala lie Asn Ala G y tagtctttgg cgttttgcgg 984 ut ari cum Arg G u Ala Val G y Cys 25 Cys Ala G n 40 Tyr Pro Il e Leu Thr Al a Thr Asp Lys Leu Al a 10 G y Ser Sy Asp Phe Al a Asp Gn Thr Arg I 90 Page 511 Hi s Al a Val Val Hi s SIle Leu Leu I le Al a Asp Phe G u Cys Phe Sy Leu I e seql i st cor r ect ed. t xt Asn Pro Asn Asn Pro Ser y Thr 100 Thr Ile Thr n Ala n Phe Asp Asn Al a G u 145 Al a G u Ser Leu Ser 225 Gy Val G u Met 115 Phe Hi s Gy I e Al a 195 G u Leu Gy Ar g Thr 275 Lys Phe Hi s Al a 165 Al a Al a Val Al a Al a 245 Phe Lys Val Asn Asp 150 Gy Met Al a G u Al a 230 G u Pr o Leu Pr o Ar g 135 Asn Leu Asn Al a G u 215 Pr o Leu G u Leu Asn 120 Al a Val Ar g Lys Leu 200 Thr Thr Al a Gy Ar g 280 105 Asp Asp SIle Val Val 185 Al a Val G n Al a Al a 265 Al a 110 Val Asp Gy Gy 170 Al a Ser G u Al a Lys 250 Ar g Tr p Val Thr Leu 155 Tyr l Ie Leu Lys Asn 235 Leu SIle G u Val Pr o 140 Ar g Al a Pr o Asn Ar g 220 Phe Al a Ser Al a Gy 125 Val Thr Phe Phe Ser 205 Asp Val G u Val l Ie 285 Leu Al a Phe Gy Al a 190 Al a Al a Tr p Hi s Thr 270 Asn Asp Thr Ser Asn 175 Val Asp Val Leu Gy 255 Asn Al a G u G u Lys 160 Al a Asn G u Val Pr o 240 I e Al a Gy <210> 397 <211> 545 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> FRXA00446 <400> 397 atg gaa aag gtt cca aac gat gtc gtt Ivet G u Lys Val Pro Asn Asp Val Val 1 5 ttt gag ttc aac cgc gcg gac gac acc Phe G u Phe Asn Arg Al a Asp Asp Thr 25 cac cgc cac gac aac gtg att ggt ttg His Arg His Asp Asn Val I e y Leu 40 ggc ctg gcg ggc ttg cgt gtt ggt tac Sy Leu Ala y Leu Arg Val y Tyr 55 gtt ggg ctg gat gag gct tat Val y Leu Asp G u Al a Tyr 10 cca gtt gcc act gag gaa atc Pro Val Ala Thr Gu u Ile cgc acg ttc tcc aag gcg tat Arg Thr Phe Ser Lys Ala Tyr gcc ttc gga aac gca gag atc Al a Phe y Asn Ala u Ile Page 512 seql i atc gca gcg atg aat aag gtg gct att lie Ala Ala IVbt Asn Lys Val Ala Ile 70 gct cag gcg gca gcg ctt gcg agt ttg Al a G n Al a Al a Al a Leu Al a Ser Leu gaa cgg gtg gag gaa acc gtc gaa aag G u Arg Val G u G u Thr Val G u Lys 100 105 ctt ggt gct gcg ccg acg cag gcc aat Leu y Ala Ala Pro Thr G n Ala Asn 115 120 ggc gcc gct gag ttg gcg gct aaa ttg G y Al a Al a G u Leu Al a Al a Lys Leu 130 135 cgc gcg ttc ccc gag ggt gcg cgc att Arg Ala Phe Pro G u G y Ala Arg lie 145 150 act gac aag ctg ctg cgc gcg tgg gag Thr Asp Lys Leu Leu Arg Ala Trp G u 165 tagtctttgg cgttttgcgg tgc <210> 398 <211> 174 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 398 IVbt G u Lys Val Pro Asn Asp Val Val 1 5 Phe G u Phe Asn Arg Al a Asp Asp Thr His Arg His Asp Asn Val I e y Leu 40 Sy Leu Ala y Leu Arg Val y Tyr 55 lie Ala Ala IVbt Asn Lys Val Ala Ile 70 Ala G n Ala Ala Ala Leu Ala Ser Leu G u Arg Val G u G u Thr Val G u Lys 100 105 Leu y Ala Ala Pro Thr G n Ala Asn 115 120 G y Al a Al a G u Leu Al a Al a Lys Leu 130 135 Arg Ala Phe Pro G u G y Ala Arg lie 145 150 st cor r ect ed. t xt cct ttc gcg gtg Pro Phe Ala Val 75 aat t ct gcc gat Asn Ser Ala Asp 90 cgt gat gct gtg Arg Asp Ala Val ttc gtc tgg ctg Phe Val Trp Leu 125 gcc gag cac ggc Al a G u Hi s G y 140 tcg gtg acc aac Ser Val Thr Asn 155 gcc atc aat gct Ala lle Asn Ala 170 t ca Ser ttg Leu t ca Ser ggc Gy gt g Val gag Gu gca Al a at g Met gcg Al a gag Gu att SIe gaa G1u 160 Val 10 Pr o Ar g Al a Pr o Asn 90 Ar g Phe Al a Ser Gy Val Thr Phe Phe Ser Asp Val G u Val 155 Leu Al a Phe Gy Al a Al a Al a Tr p Hi s 140 Thr Asp Thr Ser Asn Val Asp Val Leu 125 Gy Asn Al a G u Al a G u Ser Leu Ser Gy Val G u Thr Asp Lys Leu Leu Arg Ala Trp Gu Ala lie Asn Ala Gy Page 513 seqi i st car r ect ed. t xt 165 170 <210O> 399 <21 1> 1221 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1198) <223> RXA01105 <400> 399 t tgt cat caa ct t tgccaac gccgaagat c t tccagcgca gctttgaaaa cctccccacc accgacgagg cctaagaaaa cggcgaagca at ccgt gcac acc aaa at t act Thr Lys I Ie Thr tt g Le u ggc G y cct Pr o g at As p g aa O3u gct A a ct g Le u gga G3y c ac Hi s at g vb t 1 50 tt t Ph e g at As p g at As p ccc Pr o cca Pr o at c I Ie cgt Ar g ac c Thr c ag O31n c aa O31n 120 g aa O31u gt g ValI ac c Thr gag O31u cca Pr o ct c Le u t ca Ser ac c Thr gag O31u g at As p ct g Le u ag c Ser at t I Ie ct g Le u ccg Pr a 1 70 at c I Ie t t g Le u aac As n gag O31u gag O31u t t g Le u 75 aac As n cag O31n t at Tyr gcg A a gaa (31u 155 aac As n at c I Ie cgt Ar g gt t ValI gca A a ct g Le u gct A a ct g Le u gct A a t cc Ser gt g ValI 140 g aa (31u aac As n aac As n g aa (31u at t lIe gt c ValI cgc Ar g t ac Tyr gct A a ggt G31y 110 c ac Hi s cga Ar g cgt Ar g ac c Thr gcc A a 1 ct g Le u 15 cgc Ar g gct A a t ac Tyr at c I Ie gcc A a 95 gga G31y cca Pr a ggt G31y gca A a ggt G31y 1 75 cca Pr a cgc Ar g ct c Le u g ac As p cca Pr a ac c Thr aat As n cct Pr a at t I Ie gct A a aag Ly s 1 g at As p ggc G31y gag (31u ac c Thr gt t ValI cgc Ar g c aa O31n t cc Ser cgc Ar g gct A a 130 tt c Ph e cct Pr a ac c Thr gt g ValI gct A a g aa G u ac c Thr gct A a ggc O3y g aa O3u 1 00 gcg A a ggc O3y at c I Ie at t I Ie ct g Le u 1 gt g ValI Page 514 seql i st cor r ect ed. t xt gaa gct tat gcg gaa G u Ala Tyr Ala G u 200 ttc tcc cca tca cct tca gca Phe Ser Pro Ser Pro Ser Ala 205 acc act ctt Thr Thr Leu gag aag tac cca acc aag ctg gtg gtg G u Lys Tyr Pro Thr Lys Leu Val Val 215 220 ttt gat ttc gca ggt gga cgc ctc ggc Phe Asp Phe Ala G y G y Arg Leu G y 230 235 ttt atc gac gcc gtg atg cta gtc cgc Phe Ile Asp Ala Val IVet Leu Val Arg 250 ctg agc caa gca gcc gca atc gta gcg Leu Ser G n Ala Ala Al a lle Val Ala 265 270 ctg gga acc gtc gaa aag ctc tct gta Leu G y Thr Val G u Lys Leu Ser Val 280 285 cgc ttg gag gaa ctg ggc tac gct gtg Arg Leu G u G u Leu G y Tyr Al a Val 295 300 gtg ttc ttt gga gat ttc tcc gat cag Val Phe Phe y Asp Phe Ser Asp G n 310 315 ttg gat agg gga gtg ctc atc cgc gat Leu Asp Arg y Val Leu lie Arg Asp 330 cgc act acc at t ggt gtg cct gag gaa Arg Thr Thr Ile y Val Pro u u 345 350 gct gca gag atc atc aag ctg aac ctg Ala Ala G u Ile Ile Lys Leu Asn Leu 360 365 tga <210> 400 <211> 366 <212> PRT <213> Corynebacter i um gl ut am cum <400> 400 IVet Thr Lys I e Thr Leu Ser Asp Leu 1 5 G y G u His Ala Tyr G y Al a Pro G n 25 Asn Thr Asn G u Asn Pro Tyr Pro Pro 40 Leu Val Ala Thr Val Asp Lys lie Ala 55 G u Arg Asp Ala Val G u Leu Arg Asp 70 cgc Ar g ttc Phe 240 ccg Pr o cgt Ar g cgt Ar g cca Pr o gcg Al a 320 gga Gy gat Asp 210 at g agt NMt Ser gcc aac Al a Asn cat ctt Hi s Leu t cc gct Ser Al a 275 cgc gtg Arg Val 290 gag t cc G u Ser tgg cag Trp n gct ggg Al a G y ttt ttg Phe Leu 355 aag Lys cca Pr o t ca Ser 260 gac Asp gca Al a aac Asn gca Al a cac Hi s 340 gac Asp ct g Leu gct Al a gcg Al a 245 gcg Al a acg Thr gca Al a ttt Phe ttt Phe 325 ttg Leu gca Al a 739 787 835 883 931 979 1027 1075 1123 1171 1218 1221 t aagagagaa gaat t t t t ca Pro Leu Arg u u Leu Arg 10 Leu Asn Val Asp lie Arg Leu Ser G u Ala Leu Val Ala Asp Thr G u Leu Asn Arg Tyr Pro G u Leu Ala Ala Tyr Ile Thr 75 Page 515 Lys G n Thr y Val Al a Val Th seql i st cor r ect ed. t xt r Arg Asp Asn Leu Tr 90 p Al a Al a Asn Sy Sy Leu Asp 145 G n Val SIle Al a Thr 225 Val Tyr Hi s Val Ser 305 Al a SIle Al a Asn Thr 115 Lys Ar g Asp Ser SIle 195 Thr Ser Asn Leu Al a 275 Val Ser G n Gy Leu 355 SIle Leu Thr Asp Val 165 Asp Asp Leu Al a Al a 245 Al a Thr Al a Phe Phe 325 Leu Al a G n Phe Thr 135 Asp Val Val Al a Lys 215 Asp l Ie Ser Gy Leu 295 Phe Asp Thr Al a Leu 105 Pr o Phe Al a Thr Ar g 185 Al a Pr o Al a Al a Al a 265 Val G u Gy Gy I e 345 l le G n Tyr Al a G u 155 Asn SIle Phe Lys Gy 235 let Al a Lys Gy Phe 315 Leu Val Lys Phe let 125 Ser SIle Pr o Val Pr o 205 Val Leu Val Val Ser 285 Al a Asp Ar g G u Asn 365 Gy Pr o Gy Al a Gy 175 Pr o Pr o Ser Tyr Leu 255 Leu G u Val Hi s Val 335 Asn <210> 401 <211> 1449 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1426) <223> RXA01106 <400> 401 ggtaaacatg cgggcttaag aacttgtgtt gaggccgctt ggattcgggc accgagctcg Page 516 seql i st cor r ect ed. t xt aagaatttcg attcaacctt ttaagggaga acttttcgcc atg ttg aat gtc act IVet Leu Asn Val Thr gac Asp cca Pr o gtt Val ggc Gy gag G u tcg Ser aag Lys gag G u aat Asn gag G u 150 cac Hi s ggt Gy ct g Leu act Thr gga Gy 230 cga Ar g ggt Gy gaa G u aag Lys at t SIle gaa Gu t cc Ser ttc Phe 120 gt g Val ggt Gy ggc Gy gat Asp t at Tyr 200 cct Pr o gt a Val caa G n act Thr gt c Val gac Asp gca Al a tcg Ser cac Hi s ccg Pr o cca Pr o aac Asn cct Pr o 170 gt g Val gat Asp aat Asn act Thr aca Thr gac Asp caa G n cat Hi s gca Al a 75 at t SIle acc Thr at t SIle t ca Ser t cc Ser 155 cac Hi s tgg Tr p gac Asp at c SIle gat Asp 235 cca Pr o gt g Val aac Asn at t SIle gaa G u cgt Ar g act Thr gat Asp agc Ser 140 ctt Leu ccc Pr o gct Al a gct Al a ttt Phe 220 tct Ser t cc Ser tgg Tr p cgc Ar g 45 cgc Ar g aac Asn cgc Ar g gaa G u cgc Ar g 125 gt g Val gt g Val acc Thr gt c Val gca Al a 205 gt c Val gag G u aag Lys tct Ser ggc Gy ccc Pr o acc Thr gt c Val ctt Leu 110 gt g Val at t SIle gtt Val at t SIle ggc Gy 190 ggt Gy acc Thr gct Al a agc Ser 15 gt g Val gct Al a gcc Al a tta Leu cgc Ar g 95 t ca Ser gga Gy at g Met gcg Al a ttg Leu 175 ggc Gy ct c Leu gct Al a ggc Gy aac Asn 255 gac Asp ctt Leu gaa G u tcg Ser gat Asp 80 aag Lys cca Pr o ct g Leu aat Asn tcg Ser 160 gcg Al a ggt Gy gag G u gcg Al a cct Pr o 240 gtt Val at c SIle ccc Pr o gct Al a gt g Val ccg Pr o gtt Val ggt Gy t ac Tyr act Thr 145 cct Pr o gcg Al a cag G n cct Pr o aag Lys 225 aca Thr gcc Al a cga Ar g at a SIle gct Al a cgg Ar g ttg Leu cac Hi s ggc Gy gtt Val 130 gt c Val cct Pr o tgt Cys gcc Al a gt g Val 210 cgc Ar g gaa G u t ac Tyr cgt Ar g gt g Val ttg Leu gt g Val gt g Val gct Al a acc Thr 115 cca Pr o cca Pr o cag G n t cc Ser gt g Val 195 gat Asp ct g Leu at c SIle gat Asp gct Al a cag G n gat Asp cca Pr o cgt Ar g gag Gu 100 gt c Val ggc Sy gct Al a gct Al a at c SIle 180 gcg Al a at g Met gt c Val gct Al a ct g Leu 260 ttg Leu cct Pr o t ac Tyr gct Al a gaa G u caa G n act Thr ggc Sy caa G n gag G u 165 ttg Leu ttg Leu at c SIe agg Ar g gtg Val 245 at c SIe ctt gct gat gcc tct gcc aac gcc gtc Leu Ala Asp Ala Ser Al a Asn Ala Val 250 Page 517 agc caa gca gaa Ser G n Ala G u 265 cac gat gtc atg His Asp Val ?bet eql i st cor r ect ed. t xt gct gcg tcc gtg ctc atc act Ala Ala Ser Val Leu Ile Thr tcc gag cag ctt gcc aag Ser G u G n Leu Ala Lys 280 tca atc acg cgc aac gcc Ser Ile Thr Arg Asn Ala 295 cag agt ggc atc gtg ctt Sn Ser Gy Ile Val Leu 310 315 gcc gat caa tac gca gcg Ala Asp G n Tyr Ala Al a 330 cgc gcc gta gca gag cag Arg Ala Val Ala G u G n 345 gat ttc tca cca gta cca Asp Phe Ser Pro Val Pro 360 gtg ctg cca acc tct gga Val Leu Pro Thr Ser G y 375 cac acg ttc ctt cgc cca His Thr Phe Leu Arg Pro 390 395 ctg aag gac gtc tcg cag Leu Lys Asp Val Ser G n 410 ctt cca gcg cac ggc gaa Leu Pro Ala His G y G u 425 acc acc gac gag gcc taag Thr Thr Asp G u Ala 440 <210> 402 <211> 442 <212> PRT <213> Corynebacter i um gl <400> 402 IVet Leu Asn Val Thr Asp 1 5 lie Arg Arg Ala Leu Pro Pro lie Val n Pro Val Ala Ala Leu Asp Tyr G y Val Arg Val Pro Ala G u gac Asp gag G u 300 gt c Val gaa G u at c SIle ct g Leu t cc Ser 380 gt c Val gtt Val gca Al a gt a Val 285 cgc Ar g gac Asp cac Hi s acc Thr ggt Gy 365 gct Al a aac Asn gt c Val at c 270 aac Asn gt c Val gac Asp ct g Leu aac Asn 350 gat Asp cgt Ar g ct c Leu at c SIle cgt agg Ar g gca Al a at t SIle gaa G u 335 gcg Al a t ac Tyr ttc Phe at t SIle aac Asn 415 gca Al a gaa at c G u lie gaa gct G u Al a 305 tcc gtg Ser Val 320 atc cac Ile Hi s ggt gcg G y Al a t cc gca Ser Al a t cc gca Ser Al a 385 gaa t ac G u Tyr 400 ttt gcc Phe Al a cgc ttt Arg Phe gag G u 290 ttg Leu ggt Gy act Thr at c l Ie gga Gy 370 ggt Gy gat Asp aac Asn gaa G u 275 gcg Al a cgc Ar g at c SIle gag G u ttc Phe 355 t cc Ser ct a Leu gag G u gcc Al a aac Asn 435 cgt Ar g ggg Gy caa G n aac Asn 340 gt g Val aac Asn t cc Ser gct Al a gaa G u 420 ct c Leu 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1449 li e Ar g 430 aaaaat gaccaaaatt act ut ari cum Leu Arg G y Arg G y G y 25 Val G u Asp 40 G u Lys Phe 55 Val I e Al a G n Thr Pro Ser Lys Ser Asp 10 Thr Asp Val Trp Ser Val Leu Val n Asn Ar g y Al a u Asp His lie Arg Pro Ala Ser Ala Ala G u Asn Thr Leu Asp Page 518 seqI i st car r ect ed. t xt Se r I Ie 31u O31u lieArg Arg Val Pr o ValI O31y Tyr Thr 145 Pr o A a O31n Pr o Ly s 225 Thr A a ValI I Ie A a 305 ValI Hi s A a A a A a 385 Tyr A a ValI A a Thr 115 Pr o Pr a O31n Ser ValI 1 95 As p Le u I Ie As p I Ie 275 A a Ar g I Ie O31u Ph e 355 Ser Le u O31u A a O31u (31n Thr O31y O31n O31u 1 65 Le u Le u I Ie Ar g ValI 245 li1e As p Tyr A a ValI 325 A a G31y Hi s Thr A a 405 As p Ly s O31u As n O31u 150 Hi s G31y Le u Thr G31y 230 Le u Ser Ser Ser O31n 310 A a Ar g As p ValI Hi s 390 Le u Le u Pr o Ar g A a 135 A a O31y ValI A a O31y 215 ValI A a O31n O31u I Ie 295 Ser As p A a Ph e Le u 375 Thr Ly s Pr a Ser Ph e 120 ValI O31y O31y As p Tyr 200 Pr a ValI As p A a O31n 280 Thr O31y O31n ValI Ser 360 Pr a Ph e As p A a (31u 1 05 vb t Tyr ValI Tr p O31u 1 85 O31y O31y O31y A a O31u 265 Le u Ar g I Ie Tyr A a 345 Pr a Thr Le u ValI Hi s 425 Ser 90 Hi s Pr a Pr a As n Pr a 1 70 ValI As p As n Thr Ser 250 Hi s A a As n ValI A a 330 O31u ValI Ser Ar g Ser 410 Thr I Ie Ser Ser 1 Hi s Tr p As p I Ie As p 235 A a As p Ly s A a Le u 315 A a O31n Pr a O31y Pr a 395 O31n Thr As p Ser 140 Le u Pr a A a A a Ph e 220 Ser As n ValI As p O31u 300 ValI O31u I Ie Le u Ser 380 ValI ValI (31u Ar g 125 ValI ValI Thr ValI A a 205 ValI O31u A a vb t ValI 285 Ar g As p Hi s Thr O31y 365 A a As n ValI Le u 110 ValI I Ie ValI I Ie O31y 1 O31y Thr A a ValI A a 270 As n ValI As p Le u As n 350 As p Ar g Le u I Ie Ar g Ser O31y vb t A a Le u 1 O31y Le u A a O31y As n 255 A a Ar g A a I Ie O31u 335 A a Tyr Ph e I Ie As n 415 0 y 0 uAl a IlIe Ar g Ala Ar g 430 Page 519 seqi i st car r ect ed. t xt Phe G31u As n L eu Pr o Th r Th r As p G31u Ala 435 440 <210O> 403 <21 1> 876 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (853) <223> RXCO0930 <400> 403 acggcgcacc acacat t tgg t ggt gtat tg agct atct ct ttccggtgga tgatggaagc tagacgacga aagggagcat gggct gcgt g agaaaccat t aaa Ly s ggc G31y ac a Th r aag Ly s cgc Ar g at c I Ie t gt cys at g vb t at g vb t g aa G31u 1 50 aac As n gcg A a g aa G31u ggt G31y gcc A a cgt Ar g t ac Ty r ac c Th r aaa Ly s 120 ac c Th r g ac As p aac As n ac c Th r gcc A a g at As p aag Ly s t cc Ser gga G31y aac As n ggt G31y ac c Th r ct c Le u g at As p 1 70 c ac Hi s ct g Le u t ct Ser t ct Ser g aa G31u G31y aac As n aac As n gt c ValI 140 gct A a tt c Ph e aag Ly s at c I Ie gcg A a 45 gt g ValI g aa G31u ccc Pr o cgc Ar g tt g Le u 125 gt c ValI gt t ValI gag (31u aag Ly s aag Ly s aac As n ccg Pr o gct A a aac As n gca A a 110 ggc G31y ac c Th r ct t Le u gt t ValI gct A a 15 aac As n cca Pr o aac As n ggt G31y ggc G31y 95 gct A a gag G31u gt a ValI g aa G31u ac c Th r 1 75 gct A a gct A a at c I Ie ac g Th r g at As p ggc G31y gt t ValI ac c Th r t cc Ser c aa O31n gct A a 1 t gc cys ggc (31y at g vb t 1 aac As n g aa G31u ct t Le u aac As n gct A a gcc A a g aa G31u ggt G31y aag Ly s 145 ggt G31y gca A a at t I Ie t ct ggc cac t ca Se r 03 y Hi s Se r g ac As p gt t ValI g at As p at c I Ie g ac As p at g vb t gt t ValI t cc Ser 130 ggc G31y gct A a cca Pr o gcc A a gcg A a g ac As p g aa G31u t gg Tr p ct t Le u cgc Ar g 115 gt g ValI g at As p g aa G31u act Th r aag Ly s gca A a at g vb t cgt Ar g at g vb t at c I Ie 1 00 ac c Th r t cc Ser ct t Le u g aa G31u g ac As p 1 cag gct gtt 03 n A a ValI gac gca ctc gtg gaa Asp Ala Leu Val 3u 1 90 gaa gt a gaa gat (31uVal 31u As p 1 Page 520 t ct gaa t ca Ser G3 u Ser 200 ggt gca cgc O31y Al a Ar g 215 gat gt g caa Asp Val O31n 230 aca gag ct g Thr G3 u Leu tt c Ph e at c I Ie gt c ValI aac As n 250 seqi i st car r ect ed. t xt gca t ct gt t cag gt c ccc ct g gac gct Al a Ser Val O31n ValI Pr o Leu As p Al a 205 210 aag ct t gt g gac gcg t tg gaa gat t cc Lys Leu Val As p Al a Leu O31u As p Ser 220 225 acc aac at c gac t tg agc gat gag gt t Thr As n I I e As p Leu Ser As p 03 u Val 240 t agt tcgt at t t tccgcact ccg <210O> 404 <21 1> 251 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 404 vb t As n O31u Le u As n Al a Al a O31u G31y Ly s 145 G31y Al a I Ie Pr o Le u 225 Se r 03 y Hi s Se r Asp Al a Lys Val Al a Al a As p As p l'b t li1e O31u Ar g As p Tr p l'b t l'b t Leu I Ie 1 00 Val Ar g Thr 115 Ser Val Ser 130 03 y As p Leu Al a 03 u 03 u Pr o Thr As p 1 80 O31uVal O31u 1 95 Leu Asp Al a 210 (3 u Asp Ser Ar g Ar g I Ie Al a As n O31u Al a Tyr Ser ValI 1 65 li1e As p As p As p Lys Tr p Al a 03 y Lys 03 u Thr G31y G31y 40 Lys Lys Al a 55 Ar g Lys Ar g 70 1 1 e l'b t Ty r Cys Leu Thr l'b t Thr Lys 120 l'b t Phe Thr 135 (31u Asp Asp 150 Asn Asp Asn 03 n Al a Val Ser 03 u Ser 200 G31y Al a Ar g 215 As iDVal (31n Thr Thr 10 Phe Al a 25 31y As p Lys Lys 03 y Ser O31u (1y 90 As p As n 1 05 As n (1y Ar g Thr Val Leu 31y As p 1 70 Ar g As p 1 85 As p Phe Lys I I e As n Val Ly s Ly s Pr o Al a G31y 75 Tyr Ar g G31y G31y vb t 1 55 Le u Al a Ar g Ph e Tyr 235 Hi s Le u Ser Ser O31u G31y As n As n ValI 140 Al a Ph e Le u Al a Ly s 220 Ly s I Ie Al a ValI O31u Pr o Ar g Le u 125 ValI ValI O31u ValI Ser 205 Le u Ly s Ly s As n Pr o Al a As n Al a 110 G31y Thr Le u ValI O31u 1 ValI ValI Al a As n Pr a As n G31y G31y Al a O31u ValI O31u Thr 1 Al a O31n As p Al a I Ie Thr As p G31y ValI Thr Ser O31n Al a 1 Cys O31y ValI Al a Thr Asn I I e Asp Leu 240 Page 521 seql i st cor r ect ed. t xt Ser Asp G u Val Leu Thr G u Leu G u Asn Asp 245 250 <210> 405 <211> 547 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(547) <223> RXC01096 <400> 405 accgtgaaac aaaccggcgg tgcgtgccac actggtgccc gttttgctgt aaaagcaaca acgattaagg aagaaatctt acacatgttt cgacaatgac ct g Leu t ca Ser agt Ser gcg Al a aag Lys gt g Val gca Al a gct Al a acc Thr gca Al a at g Met acc Thr gca Al a at g Met gcc Al a cgg Ar g t cc Ser 120 cat Hi s ggc Gy tgg Tr p tct Ser gct Al a cgg Ar g ct g Leu cga Ar g acc Thr ct g Leu at t SIle gt a Val at t SIle tt g Leu cgc Ar g 75 tct Ser acc Thr ct g Leu gcg Al a gga Gy acc Thr gt g Val ct c Leu at c SIle cca Pr o ct g Leu ct g Leu gca Al a 140 gct Al a at c SIle ggt Gy 45 gct Al a at t SIle ct c Leu ct g Leu tct Ser 125 gt g Val ggc Gy gag G u gca Al a gcc Al a ggt Gy gcg Al a acc Thr 110 gat Asp gt a Val gcc Al a 15 gct Al a acc Thr ttc Phe ggt Gy ctt Leu 95 t cc Ser tgg Tr p gcc Al a ct g Leu ttc Phe tgg Tr p gcc Al a at t SIle ct c Leu ggt Gy gcg Al a at g Met at g Met 1 gtt Val gac Asp tct Ser gcc Al a tcg Ser acc Thr gt g Val gag G u at t SIle 145 gt c Val gat Asp aca Thr gcg Al a gcg Al a caa G n gcc Al a at c SIle 130 ggc Gy tgg Tr p aaa Lys gaa Gu ttg Leu ct g Leu gac Asp t ca Ser 115 at c SIle tgc Cys at c I e t ca Ser at c SIe gtg Val at c SIe cca Pr o 100 cag G n aat Asn gcg Al a aag cca cgc Lys Pro Arg <210> 406 <211> 149 <212> PRT <213> Corynebacter i um gl ut am cum <400> 406 IVet Lys Pro Arg Val Leu Ser Ala Leu Gy lie Gy Ala Gy Ala Leu 1 5 10 Page 522 seql i st cor r ec Val Val Trp Ile Ser Ser Arg IVbt Asn Trp Val 25 Asp Asp Lys Ser Gy Ser Val Thr G n Ser lie 40 Ser Thr G u Ile IVet Al a Leu Ala Leu Ala Leu 55 Ala Ala Leu Val Leu Lys Arg IVbt G y Arg Arg 70 Ser Ala Leu Ile Ala Val y Ala Ser Leu Ser 90 Thr G n Asp Pro Asp Al a G u Arg Ala Arg Thr 100 105 Val Ala Ser G n Lys Al a Asn Ser y Thr Leu 115 120 Su lie lie Asn Thr Thr Thr His Pro Leu Ala 130 135 I e G y Cys Al a Leu 145 <210> 407 <211> 1020 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(997) <223> RXC01656 <400> 407 tcggcaagca tggcttcatc gtcctcaaaa aagtggtcta atctaccgct agtccacttt gtggcgt t gg at catctgt c ted. txt Thr Ile Val G y Leu Al a lie Ile Pro Leu Leu Leu Leu Ser 125 Al a Val 140 G u Al a Al a Gy Al a Thr 110 Asp Val Al a Thr Phe Gy Leu Ser Tr p Al a gaa G u ct c Leu at c SIle ccc Pr o gac Asp aaa Lys t ac Tyr ggt Sy gaa G u gat Asp at c SIle cgc Ar g gca Al a gt g Val gca Al a cgt Ar g gga G y ggt Sy act Thr at g Met gcc Al a cag G n gt c Val ttc Phe gt g Val gt g Val gca Al a ggc G y gcg Al a gaa G u aag cgc Lys Ar g 15 gtc acc Val Thr gtt atg Val [Vbt gtt gct Val Ala gtc t cc Val Ser 80 gct cag Al a G n 95 Page 523 atgcaagtga ctgaaagtgg atg acc gaa act caa Met Thr G u Thr G n 1 ggc ctt gcc gac atg G y Leu Al a Asp IVet cct gaa caa gcg cgc Pro G u G n Ala Arg gca ctc gag cgc gtt Ala Leu G u Arg Val cgc atg agt gat cct Arg IVet Ser Asp Pro atc ccg gtc atg gcg Ile Pro Val lVbt Ala gtt ctg gaa gct ctc Val Leu G u Ala Leu 100 seql i st cor r ect ed. t xt ggt gtt gat ttc atc gac gag tcc gaa gtt ctc agc cct gcc gac tac 451 Sy Val Asp Phe I e Asp u Ser G u Val Leu Ser Pro Ala Asp Tyr 105 110 115 acg cac cac atc aac aag tgg aag ttc gac gtt cct ttc gtc tgt ggc 499 Thr His His lie Asn Lys Trp Lys Phe Asp Val Pro Phe Val Cys y 120 125 130 gcg acc aac ctc ggc gaa gct ttg cga cgc atc acc gaa ggc gct gca 547 Ala Thr Asn Leu G y u Ala Leu Arg Arg lie Thr u Gy Ala Ala 135 140 145 atg atc cgt tcc aag ggc gaa gcc ggc acc ggc gat gtc tct gaa gct 595 IVet Ile Arg Ser Lys Gy u Ala Gy Thr Gy Asp Val Ser u Ala 150 155 160 165 gtc cgt cac ctg cgc acc atc cgc ggc gac atc aat cgc ctg cgc tcc 643 Val Arg His Leu Arg Thr lie Arg Gy Asp lie Asn Arg Leu Arg Ser 170 175 180 ctg gat gag gat gaa ctc ttc gtc gcc gcc aag gaa ttc cag gca cca 691 Leu Asp G u Asp G u Leu Phe Val Ala Ala Lys G u Phe G n Ala Pro 185 190 195 tac gac ctg gtc cgc gaa gtc gcc tcc acc ggc aag ctc cct gtg gtc 739 Tyr Asp Leu Val Arg G u Val Ala Ser Thr y Lys Leu Pro Val Val 200 205 210 acc ttc gtt gca ggt ggc gtc gca acc cca gcc gac gct gca ctc gtg 787 Thr Phe Val Ala Gy G y Val Ala Thr Pro Ala Asp Ala Ala Leu Val 215 220 225 cgc caa atg ggc gcc gaa ggc gtc ttt gtc ggc tcc ggc atc ttc aaa 835 Arg n IVet Gy Ala u y Val Phe Val y Ser Gy Ile Phe Lys 230 235 240 245 tcc ggc aat cca gcc gcc cgc gcc gca gcg atc gtc aag gct gca acg 883 Ser y Asn Pro Ala Al a Arg Ala Ala Ala lie Val Lys Ala Ala Thr 250 255 260 ctt ttc gac gac ccc tcc gtc att gcc gac gta tcc cgc ggc ctg ggt 931 Leu Phe Asp Asp Pro Ser Val Ile Ala Asp Val Ser Arg y Leu y 265 270 275 gaa gcc atg gtg ggc atc aac gta tcc gac gtt cca gca cca cac cga 979 G u Ala Iet Val G y Ile Asn Val Ser Asp Val Pro Ala Pro His Arg 280 285 290 ctc gcc gag cgc ggc tgg tgatcgttgg agttttagct ctc 1020 Leu Ala G u Arg G y Trp 295 <210> 408 <211> 299 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 408 Met Thr G u Thr n G u Thr Tyr G n Ala Thr Thr Arg Val Lys Arg 1 5 10 G y Leu Al a Asp IVet Leu Lys G y G y Val I e IVet Asp Val Val Thr 25 Pro G u G n Ala Arg I le Ala u Asp Al a y Ala Ser Ala Val Iet 40 Page 524 seqI i st car r ect ed. t xt Ala Leu Ou Arg Val Pro Ala Asp Ilie Arg Ser On Gy Gy Val Ala Ar g lie ValI Ser Pr o Thr 145 As p As n O31u Ly s As p 225 Ser ValI Ser Pr o Ser ValI O31u Al a 115 ValI G31y Ser Le u O31n 1 95 Pr o Al a I Ie Al a G31y 275 Pr o Pr a Al a Le u Tyr G31y Al a Al a 1 65 Ser Pr a ValI ValI Ly s 245 Thr G31y Ar g Le u Al a ValI Hi s Thr 135 li1e Ar g As p As p Ph e 215 O31n G31y Ph e Al a Al a 295 Ou le Ph e 1 05 li1e Le u Ser Le u As p 1 85 ValI Al a G31y Pr a As p 265 ValI Ar g G31y G31y 90 li1e As n G31y Ly s Ar g 1 70 G u Ar g G31y Al a Al a 250 Pr a G31y G31y lIe Hi s As p Ly s (31u G31y 1 Thr Le u (31u G31y (31u 235 Al a Ser I Ie Tr p As n ValI Ser Ly s 125 Le u Al a Ar g ValI Al a 205 Al a ValI Al a I Ie ValI 285 Al a (31u (31u 110 Ph e Ar g G31y G31y Al a 1 Ser Thr Ph e Al a Al a 270 Ser ValI Al a ValI As p Ar g Thr As p 1 Al a Thr Pr a ValI Al a 255 As p As p <210O> 409 <21 1> 1065 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1042) <223> RXCO1158 <400> 409 t aggacgt tc aaggaat tgg ct gaat caac aagcgccaag gt ggt taagc gccct cggcg agt tat ctca aaaaagaaga agaagt ct cc t acgggagag at g t cc at t gt t gag 115 I'bt Ser I Ie Val O31u 1 cac at c aaa gag t tt cga cgc cga ct t ct t at c gct ct g gcg ggc at c 163 Page 525 seql i st cor r ect ed. t xt His lie Lys u Phe Arg Arg Arg Leu Leu lie Ala Leu 15 Al a y l e ct c Leu cag G n cct Pr o ct c Leu gcg Al a tgg Tr p gca Al a ctt Leu ggt Sy 150 ttc Phe ct g Leu at t SIle gct Al a ctt Leu 230 aac Asn gac Asp ggc G y ccc Pr o gaa Gu acc Thr gt g Val ttt Phe ttc Phe 120 t ac Tyr gac Asp ct c Leu at c SIle gat Asp 200 at g Met ct c Leu aaa Lys tct Ser at t SIle ttg Leu cgc Ar g ccg Pr o at g Met acc Thr acg Thr gt c Val cag G n ttg Leu 170 at g Met cga Ar g ccc Pr o gtt Val cgg Ar g 250 t ca Ser at c SIle ggc Gy tgg Tr p ttt Phe 75 gtt Val cca Pr o at t SIle gtt Val gca Al a 155 tt g Leu ct c Leu cgc Ar g ggc Gy ct g Leu 235 gac Asp cca Pr o ggc G y gag G u gcc Al a gat Asp ctt Leu ggt Gy gct Al a gca Al a 140 gcg Al a gcg Al a aac Asn at g Met cag G n 220 gt a Val aag Lys ct g Leu ttt Phe ct g Leu 45 at g Met cca Pr o ggc Gy ttg Leu gtt Val 125 t at Tyr gcc Al a at t l Ie at t SIle at c SI e 205 gat Asp gag G u aag Lys gat Asp at t I e ct g Leu agc Ser ttc Phe t ca Ser at g Met 110 gt g Val ggt G y ct g Leu ttc Phe gt g Val 190 at c SIle cct Pr o ctt Leu cgc Ar g act Thr 270 tgg Tr p agg Ar g gac Asp at g Met ccc Pr o 95 aag Lys ct g Leu ttg Leu act Thr ggc G y 175 ggt G y at g Met ttc Phe gcc Al a cca Pr o 255 tct Ser t ac Tyr gat Asp t ca Ser ctt Leu 80 gt g Val aat Asn ttt Phe gag G u ggt G y 160 gt g Val at c SIle at t SI e acc Thr ct g Leu 240 gaa G u gct Al a ttc Phe t ac Tyr gaa G u ctt Leu ct g Leu cgc Ar g ggc G y 130 ct c Leu aag Lys ttc Phe cct Pr o ttc Phe 210 ttg Leu ttc Phe ctt Leu gga Gy t ca Ser tgt Cys tgt Cys aaa Lys agc Ser cgt Ar g 115 ggt G y ctt Leu t ac Tyr gaa G u t ac Tyr 195 gt g Val gt g Val tgt Cys gat Asp gaa G5u 275 ttt Phe tct Ser cga Ar g gt a Val cag G n 100 t ac Tyr gct Al a acc Thr ttc Phe gtt Val 180 gat Asp ttc Phe ttg Leu cgc Ar g ggc Gy 260 gat Asp tgg Tr p ctg Leu ctg Leu gcg Al a ctg Leu acc Thr gtt Val att SIe gga G y 165 cca Pr o gcc Al a gct Al a gcg Al a ttc Phe 245 gat Asp gct Al a cca agc cca gtc gaa acc cca gag gcg gtg gag cct tcg cgg atg ctg Page 526 seql i st cor r ect ed. t xt Pro Ser Pro Val u Thr Pro G u Ala Val G u Pro Ser Arg Met Leu 280 285 290 aac cca agt ggg gag gcg tcg ata agc tat aaa ccc ggg cgc gcc gac Asn Pro Ser Gy G u Ala Ser Ile Ser Tyr Lys Pro Gy Arg Ala Asp 295 300 305 ttc ggt gac gtg ctc tagggcctag ccaggtaccc tta Phe G y Asp Val Leu 310 <210> 410 <211> 314 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1027 1065 <400> 410 Mat Ser Ile Val 1 Al a Asp Pr o G u Ar g Tr p G u Val Phe 145 Asp Ser Leu Leu Met 225 G n Tr p Al a Ser Cys Cys Lys Ser Ar g 115 Gy Leu Tyr G u Tyr 195 Val Val Cys Asp G u His Il e 5 II e Leu Val Trp n lie Leu Pr o Al a Leu Leu Al a 70 Al a Al a Leu Leu Trp G y Thr Ala lle Val Leu Al a 135 lie y G y 150 G y Phe Leu 165 Pro Leu Val Al a I e Lys Al a Al a Phe 215 Ala Leu Ser 230 Phe Asn Asp 245 Asp Asp Leu G u Phe 10 Thr Ile Thr Leu Ser Arg G y Pro Sy let 90 lie Thr 105 Val Thr Phe Val Thr G n Al a Leu 170 Sy let 185 Lys Ar g Thr Pro Thr Val Arg Arg 250 Al a Ser 265 Ar g SIle Gy Tr p Phe Val Pr o SIle Val Al a 155 Leu Leu Ar g Gy Leu 235 Asp Ar g Gy G u Al a Asp Leu Gy Al a Al a 140 Al a Al a Asn bet G n 220 Val Lys Ar g Phe Leu Mt Pr o Gy Leu Val 125 Tyr Al a l Ie SIle SI e 205 Asp G u Lys Leu Tr p Ar g Asp Met Pr o Lys Leu Leu Thr Gy 175 Gy bt Phe Al a Pr o 255 Pro Leu Asp Thr Ser Ala 270 Page 527 seqi i st car r ect ed. t xt Gy OyOu AspAla Pro Ser ProVal Gu Thr ProOu AlaVal Gu 275 280 285 Pr o Ser Ar g l'bt Leu Asn Pr o Ser G31y G31u Al a Se r I Ie Ser Tyr Lys 290 295 300 Pro G31y Ar g Ala Asp Phe G31y Asp Val Leu 305 310 <210> 411 <211> 1413 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221> CDS <222> (1390) <223> RXA02458 <400> 411 t tgcggagcc acgact gat c ggt caagt gc t at ctcggt t gcat caat aa gcgaat cacc ggt ggt gaac aaaacaaat c t tccacacat aacat ctat t g at As p ccc Pr a cgc Ar g g at As p ag c Ser gag G31u ac g Thr gt t ValI at t I Ie ct g Le u 1 50 t ct Ser gt c ValI ct c Le u ct t Le u gga G31y gga G31y at g vb t tt t Ph e 120 g at As p tt c Ph e t ct Ser g ac As p tt g Le u agt Ser ac c Thr tt g Le u tt t Ph e ggc G31y ct g Le u gt t ValI ccc Pr a gct Al a gcg Al a g at As p act Thr gct Al a cct Pr a cct Pr a t cg Ser 140 gct Al a t gg Tr p cct Pr a gca Al a g at As p gag G31u t cc Ser gt t ValI 110 gct Al a ggt G31y gag G31u g at As p 15 ggt G31y t ca Ser ct c Le u gca Al a gt t ValI 95 gct Al a cgt Ar g gt g ValI gt c ValI gct Al a t cc Ser act Thr at g vb t gt c ValI gag G31u gct Al a gt t ValI gag G31u cct Pr a 1 at g vb t 1 ccg Pr a aag Ly s cca Pr a act Thr g at As p t gt cys tt c Ph e cgt Ar g gt a ValI 145 gag G31u cgc Ar g t cg Ser t cc Ser g at As p cgc Ar g ggt G31y gct Al a ccg Pr a 130 g ac As p ggt G31y g tc t tt gt g Val Phe Val gct Al a at c I Ie ac c Thr ggt G31y t ac Tyr ct t Le u g at As p 115 at g vb t aac As n ggc G31y cgc Ar g ac c Thr at c I Ie ct a Le u cgc Ar g gct Al a 1 00 ggt G31y ac c Th r aac As n gt g ValI Page 528 seql i st cor r ect ed. t xt gag att gat gct tcc ggc tca tct cag ttt gtt tct ggt ctt ttg ctt 643 G u Ile Asp Ala Ser G y Ser Ser G n Phe Val Ser y Leu Leu Leu 170 175 180 tca gcg cct cgt ttt aaa aat ggc gtc acc gtt aag cac gtc ggt ggt 691 Ser Ala Pro Arg Phe Lys Asn G y Val Thr Val Lys His Val G y G y 185 190 195 cgt ctg ccg agc atg ccg cat att gag atg acc gtc gat atg ctt cgt 739 Arg Leu Pro Ser IVet Pro His lie G u IVet Thr Val Asp IVet Leu Arg 200 205 210 tcc gca ggc att gag atc gaa gag tca gaa aat cag tgg gtt gtt cat 787 Ser Ala Gy Ile G u Il e G u Gu Ser G u Asn G n Trp Val Val His 215 220 225 cct ggt gag atc ttg ggt cgg acc tgg cgc att gag ccg gat ctt tct 835 Pro Gy G u lie Leu G y Arg Thr Trp Arg lie G u Pro Asp Leu Ser 230 235 240 245 aat gcg act ccg ttc cta gct gcc gct gcg gtc act ggt gga acc atc 883 Asn Ala Thr Pro Phe Leu Ala Ala Ala Ala Val Thr Gy y Thr Ile 250 255 260 aag att aac cac tgg cca atc aaa act act cag cct ggc gat gct att 931 Lys IIe Asn His Trp Pro lie Lys Thr Thr G n Pro G y Asp Ala lie 265 270 275 cgt tcg att ctt gag cgc atg ggc tgc gaa gtt gag ctg gtt gct cag 979 Arg Ser Ile Leu G u Arg Iet y Cys G u Val G u Leu Val Ala G n 280 285 290 ggt gaa ggt tac gat ctg tcg gtg act ggt ccg gtt gct ctc aag ggc 1027 Gy G u y Tyr Asp Leu Ser Val Thr y Pro Val Ala Leu Lys y 295 300 305 att gag atc gat atg tcc gat atc ggt gag ttg acc cct acc gtg gcg 1075 lie Gu lie Asp IVet Ser Asp lie Gy u Leu Thr Pro Thr Val Ala 310 315 320 325 gcg ttg gct gcg ttg gcg tcg aca gag tct cgt ttg acc ggt att gct 1123 Ala Leu Ala Ala Leu Al a Ser Thr u Ser Arg Leu Thr Gy Ile Ala 330 335 340 cat ctt cgt ggc cat gag acg gat cgt ttg gct gcg ttg act gcg gag 1171 His Leu Arg G y His G u Thr Asp Arg Leu Ala Ala Leu Thr Ala G u 345 350 355 atc aac aaa ctt ggt gga aag tgc act gag ctt aag gat ggt ctg ttg 1219 I e Asn Lys Leu G y G y Lys Cys Thr G u Leu Lys Asp G y Leu Leu 360 365 370 att gag cct gcg tcg ctg cac ggt ggt gtg tgg cat tca tat gct gat 1267 lie G u Pro Ala Ser Leu His G y G y Val Trp His Ser Tyr Ala Asp 375 380 385 cac cgt atg gct act gct ggt gcg atc att ggc ctc gcg gtt gat ggc 1315 His Arg IVet Ala Thr Ala G y Ala lie ile G y Leu Ala Val Asp G y 390 395 400 405 gtt cag gtt gaa gac att aag acc act tcc aaa act ttc cct ggt ttt 1363 Val G n Val G u Asp Ile Lys Thr Thr Ser Lys Thr Phe Pro y Phe 410 415 420 gaa aat gtt tgg gag gag atg gtt ggc tagacgcagc tatgacgaat 1410 Su Asn Val Trp u u IVet Val y 425 430 Page 529 seqI i st car r ect ed. t xt ccg <210O> 412 <21 1> 430 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1413 <400> 412 rvb t ValI P Pr o Ar g A Lys Ser I Pr o Ser T Thr As p C3 Asp Arg T Cys (1y L Phe Al a A Ar g Pr o 130 Val Asp A 145 31 u 31y3Ser G3 y L Lys Hi s V Val Asp 210 O31n Tr p V 225 O31u Pr o A Thr G3 y C3ro (3 2 G3 u Leu V 290 Val Al a L 305 he Val I a Ar c 2C 1 e Thr hr lI E y LeL yr Ar c eu Al E 1 OC Sp a31 bt Thr Sn As r Iy Val e u LeL 1 8C al31 bt LeL aI Val sp LeL y Thr 26C sp Al E 75 a1 Al E eu LvE Ser Asp Ser G31y Pr o I Ie As n Ar g Al a I I e As p Val 55 Arg Ser Leu 70 Val O31u Pr o 03 y Thr Val Pr o Val Hi s Ser Ilie Leu 135 Asn Leu Pro 150 Val O31u Ilie 1 65 Leu Ser Al a G31y Ar g Leu Ar g Ser Al a 215 Hi s Pr o (31y 230 Ser Asn Al a 245 Il e Lys Il e Ilie Ar g Ser (31n (1y (1u 295 3y Ilie (3u 310 Ser I Ie Ser 10 Val Ser Asp 25 Leu Il e Leu 40 Leu Arg Ser 03 y Il e Thr 03 y 03 n Leu 90 I'b t Ar g Phe 1 05 Phe Asp 03 y 120 Asp Al a Leu Phe Thr Val Asp Al a Ser 1 70 Pr o Ar g Phe 1 85 Pr o Ser I'b t 200 G31y Ilie O31u 03 u Il e Leu Thr Pr o Phe 250 Asn Hi s Tr p 265 Il e Leu 03 u 280 a3 y Tyr Asp Il e Asp k~bt Le u Le u Al a Ar g I Ie Ser Le u As p Ar g As n 1 G31y Lys5 Pr o I Ie G31y 235 Le u Pr o Ar g Le u Pr o Al a Al a As p Thr Al a Pr o Pr o Ser 140 Al a Ser As n Hi s 031u 220 Ar g Al a I Ie vb t Ser 300 le le Le u Thr O31u G31y Pr o O31n 125 Le u G31y Ser Gy le 205 O31u Thr Al a Lys5 G31y 285 ValI Tr p Pr o Al a As p O31u Ser ValI 110 Al a G31y O31u O31n ValI 1 (31u Ser Tr p Al a Thr 270 Cys Thr As p G31y Ser Le u Al a ValI Al a Ar g ValI ValI Ph e 1 Thr vb t (31u Ar g Al a 255 Th r (31u G31y Ser Asp Ii e 0y 0 u Leu 315 320 Page 530 Thr Le u Al a Ly s Hi s 385 Le u Thr Thr G31y Thr 355 G31y Tyr ValI Pr o Al a 325 Al a O31u Le u As p G31y 405 Ph e Al a Hi s I I e I I e Hi s 390 ValI (31u Le u Le u As n O31u 375 Ar g O31n As n seql i st car r ect ed. Al aAl a Leu Al a Ser 330 Ar g O31y Hi s O31u Th r 345 Lys Leu 03 y 03 y Lys 360 Pr o Al a Ser Leu Hi s 380 l'b t Al a Thr Al a 03 y 395 Val O31u As p I Ie Lys 410 Val Tr p O31u O31u [Vbt 425 t xt Thr As p cys 365 O31y Al a Thr ValI Ser 335 Le u O31u ValI I Ie Ser 415 <210O> 413 <21 1> 1266 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1243) <223> RXA02790 <400> 413 tggatgctcg cacccgagcc cttgaaccac aatccacaga agggaaattt cccaggatga accaaatccg aaaccgccgg cacccaagat ttcgacgaga gag ccc gt c O31u Pro Val gt a ValI ct c Le u ac g Thr g aa O31u g aa O31u aac As n g aa (31u cgc Ar g at c I Ie cat Hi s cct Pr o g at As p ccc Pr o at g vb t c aa O31n gcc Al a t at Tyr ggt G31y gt g ValI gcg Al a gca Al a cgg Ar g at c I Ie gaa O31u t cc Ser gcc Al a 75 ggt G31y gca Al a tt t Ph e at c G31y t cc Ser ac c Thr agt Ser gct Al a at t I Ie t at Tyr t cc Ser 45 at a I Ie aac As n gt a ValI gca Al a gcc Al a at c I Ie gga G31y t ca Ser t ca Ser c ag O31n gt g ValI 110 gt g ValI 15 ggt G31y aac As n gag (31u gcg Al a cca Pr o 95 t cg Ser ac g Thr gt a ValI ggt G31y ct c Le u tt c Ph e 80 ggt G31y gct Al a gct Al a ac c Thr gt t ValI cca Pr a ac a Thr t ac Tyr ct t Le u t ca Ser t ca Ser c ag O31n gag O31u gcg Al a cgt Ar g g at As p 115 ct c Le u aac As n ct g Le u tt g Le u gcc Al a ct g Le u 1 00 cca Pr a t ac Tyr at c Ar g gt c ValI g aa O31u ag c Se r c ag O31n g ac As p Page 531 seql i st cor r ect ed. t xt aac cag gtt gat ctc ctc gac att cac ggc ggc gcc Asn G n Val Asp Leu Leu Asp lie His Gy Gy Ala 120 125 acc ttg atg gac Thr Leu IVet Asp gt c act Val Thr 135 gca gcc Al a Al a 150 gat ttg Asp Leu cca gat Pro Asp cgc ct c Arg Leu aac gac Asn Asp 215 cat ttc Hi s Phe 230 act cca Thr Pro cca gca Pro Al a gcc gag Al a G u tct gaa Ser G u 295 cca tgg Pro Trp 310 gca gt a Al a Val aac tgg Asn Trp aac gac Asn Asp agt ggc Ser G y 375 gtt Val gt g Val cag G n tgg Tr p gaa G u 200 gcc Al a caa G n t at Tyr gga Sy cca Pr o 280 caa G n aac Asn t cc Ser ct g Leu acc Thr 360 ggc Gy tgc Cys gtt Val 170 at c SIle ct g Leu gga Gy acc Thr ct g Leu 250 ttt Phe cag G n gt a Val t ac Tyr gaa G u 330 ttt Phe gaa G u gga Gy acc Thr 155 gcc Al a gct Al a at c SIle at c SIle gac Asp 235 gt c Val gat Asp ct g Leu gca Al a gcc Al a 315 gca Al a gt c Val gag G u aac Asn 140 gaa G u t cc Ser gct Al a at g Met ct c Leu 220 at c SIle acc Thr aag Lys caa G n acc Thr 300 at g Met ct c Leu acc Thr cac Hi s acc Thr ggc G y acc Thr gt g Val cct Pr o 205 acc Thr acg Thr gca Al a gt c Val ttc Phe 285 acc Thr gac Asp caa G n at c SIle gaa 35u 365 cgc Ar g t cc Ser gt g Val gaa G u 190 ggc G y gat Asp ggc Sy gca Al a gcc Al a 270 gac Asp gac Asp ggc G y gcc Al a gac Asp 350 gcc Al a gcg Al a gcc Al a tcg Ser 175 gct Al a caa G n ct g Leu cgt Ar g tct Ser 255 cgc Ar g t cc Ser gaa G u ct g Leu at g Met 335 acc Thr gac Asp gga at c G y lle 145 aac tgc Asn Cys 160 cct gca Pr o Al a cgc gga Arg G y tac gtg Tyr Val atc acg Ile Thr 225 gca gat Al a Asp 240 t t a at c Leu II e gt c at c Val lile acc gt c Thr Val gac cgt Asp Arg 305 cca caa Pro G n 320 gaa aac Su Asn gat gga Asp y att gag Ile G u 130 tac t cc Tyr Ser atc acc Ile Thr gaa ttg G u Leu act gat Thr Asp 195 gtg gat Val Asp 210 cga t ca Arg Ser gcc at c Ala I e gag cgc G u Arg ttg aac Leu Asn 275 aac t ac Asn Tyr 290 cag acc G n Thr acc ccc Thr Pro cct gca Pro Al a acc acc Thr Thr 355 caa gct G n Al a 370 cag G n gct Al a ggt G y 180 cca Pr o cca Pr o gca Al a gga Sy gaa G u 260 cgt Ar g ggt Sy gt c Val at c SIle gaa G u 340 gt g Val ttg at c SIe gag G u 165 gt c Val aag Lys t cc Ser aac Asn ctt Leu 245 gca Al a ct c Leu ct g Leu acc Thr gcc Al a 325 gga G y ttc Phe aac 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1266 Leu Asn gtt cta gac agc Val Leu Asp Ser aac cga Asn Arg 380 taaggatcag cgaataaaat tgg Page 532 seqI i st car r ect ed. t xt <210O> 414 <21 1> 381 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 414 I'bt O u Pro Val le Al a ValI O31y O31n Ph e Le u Al a I Ie 145 CYS Al a O31y ValI Th r 225 As p I Ie I Ie ValI Ar g 305 Ser Ser O31n O31u Al a Ar g As p 115 Le u Ser Th r Le u As p 1 95 As p Ser I Ie Ar g As n 275 Ty r Th r Tyr Val Lys 1 1 e Leu I I e Ar g Thr Pr o Val O31u I Ie O31u O31u Ar g 70 Ser As n As n G n Gu Gn As p As n O31n As p Val Thr 135 1I e Al a Al a 150 O3 u Asp Leu 1 65 Val Pr o Asp Lys Arg Leu Ser Asn Asp 215 Asn Hi s Phe 230 Leu Thr Pro 245 Al a Pr o Al a Leu Al a 03 u Leu Ser 03 u 295 Thr Pr o Tr p 310 Ar g O31y As p O31u I Ie As n As n 1 05 As p ValI Thr O31n Al a 1 85 G31y O31n O31u O31u As p 265 vb t O31u Thr O31n 10 Al a Tyr O31y ValI Al a 90 Al a Le u O31y cys ValI 1 70 li1e Le u O31y Thr Le u 250 Ph e O31n ValI Tyr Ar g I Ie O31u Ser Al a 75 O31y Al a Le u O31y Thr 1 55 Al a Al a I Ie I Ie As p 235 ValI As p Le u Al a Al a 315 Ph e I Ie O31y Ser Thr Ser Al a As p As n 140 O31u Ser Al a vb t Le u 220 li1e Thr Ly s O31n Thr 300 vb t I Ie Tyr Ser li1e As n ValI Al a I Ie 125 Thr O31y Thr ValI Pr o 205 Thr Thr Al a ValI Ph e 285 Thr As p Al a I Ie O31y Ser Ser O31n ValI 110 Hi s Ar g Ser ValI O31u 1 O31y As p O31y Al a Al a 270 As p As p O31y ValI O31y As n O31u Al a Pr o Ser O31y Al a Al a Ser 1 Al a O31n Le u Ar g Ser 255 Ar g Ser O31u Le u Thr ValI O31y Le u Ph e O31y Al a O31y O31y As n 1 Pr o Ar g Tyr I Ie Al a 240 Le u ValI Th r As p Pr a 320 On Thr Pro Ile AiaAaVai Ser Thr Ou AlaLeu On Ala Ibt Ou Page 533 seqI i st car r ect ed. t xt 325 330 335 As n Pr o Ala O31u 31y Asn Trp Leu Tyr Phe Val Thr Ilie Asp Thr Asp 340 345 350 O3 y Th r Thr Val Phe Asn Asp Thr Phe 03 u 03 u Hi s 03 u A a Asp I I e 355 360 365 O31u O31n Ala Leu Asn Se r G31y Val Leu Asp Ser Asn Ar g 370 375 380 <210> 415 <211> 644 <212> DNA <21 3> Cor ynebact er i urn gl ut ani cumn <220> <221 OIDS <222> (621) <223> RXN00954 <400> 415 gct A a gcg A a aaa Ly s cgc Ar g ct c Le u gt g ValI gt g ValI cct Pr o G31y ac c Thr 145 at g vb t aag Ly s aac As n ccc Pr o gag O31u gcc A a ggc G31y gag O31u g ac As p 115 ggc G31y cct Pr o t ac Tyr tt c Ph e gcg A a at c I Ie c ag O31n gt c ValI ggc O31y aaa Ly s ggc O31y gag O31u gca A a aac As n 1 65 gaa O31u at t SI e t t c Ph e at c SI e t t c Ph e 70 acc Thr gaa O31u ct t Le u aac As n cac Hi s 150 ggc O31y gcg A a gcg A a aac As n at g vb t cgc Ar g g at As p g ac As p ggc O31y gcc A a 135 cgt Ar g g at As p aac As n gt g ValI ct t Le u gt g ValI tt g Le u at c I Ie ac c Thr 1 05 t ac Tyr gct A a gcg A a g ac As p tt c Ph e 10 c ag O31n gga G31y gcc A a ggc G31y gca A a 90 at c I Ie ac c Thr at g vb t tt g Le u t cc Ser 1 70 ac c Thr ccg Pr o cca Pr a aat As n cgt Ar g 75 gt c ValI gag (31u ct t Le u cgc Ar g gct A a 1 55 tt g Le u tt c Ph e gt t ValI tt g Le u gcc A a ac a Thr c ac Hi s cat Hi s gag (31u gct A a 140 gcg A a aaa Ly s ct g Le u cgc Ar g ct g Le u aat As n cgc Ar g ggc G31y t ac Tyr g at As p 125 act Thr t cc Ser g at As p tt c Ph e c ag O31n t cc Ser cat Hi s gcg A a ac c Thr ac c Thr 110 ct c Le u tt c Ph e gca A a ggt (31y gca A a gcg A a ccg Pr a gga O31y ct t Le u ac c Thr at c I Ie gt a ValI gcg A a ggt O31y gca A a 1 cct Pr a ct g Le u gcg A a c ag O31n gt t ValI tt g Le u gag O31u ggt O31y O31y gcg A a 1 c aa O31n aag gcg ct t t cct t gct t gcc gac ggc acc acc cag gca tggt t ggcc Page 534 seqi i st car r ect ed. t xt Lys Ala Leu Ser Leu Leu Ala Asp G31y Thr Thr G31n Ala Tr p Leu Ala 180 185 190 aag cac gaa gag at c gat t ac tca gaa aag gag t ct t cc aat gac Lys Hi s a u au I I e Asp Tyr Ser a3 u Lys G3 u Ser Ser Asn Asp 195 200 205 t agt aat aat ct gcccacag t gt <210> 416 <211> 207 <21 2> PRT <21 3> Cor ynebact er i urn gl ut anm curn <400> 416 A a Val Lys Tr p Phe a31u Ala Ser As n A a Ly s Ar g Le u ValI ValI Pr a a31y Thr 145 vb t Ly s Ly s As n Pr a G u A a ay au As p 115 a3y Pr a Tyr Le u a3u 1 95 A a I Ie G n ValI a3y Ly s ay au A a As n 1 65 Le u I Ie A a As n vb t Ar g As p As p a31y A a 135 Ar g As p A a Tyr ValI Le u ValI Le u I Ie Thr 1 Tyr A a A a As p a31y 1 a31u Ph e 10 a31n a31y A a a31y A a 90 lie Thr vb t Le u Ser 1 70 Thr Ly s Thr Pr a Pr a As n Ar g ValI a31u Le u Ar g A a 1 Le u Thr a31u Ph e ValI Le u A a Thr Hi s Hi s a31u A a 140 A a Ly s a31n Ser Le u Ar g Le u As n Ar g a31y Tyr As p 125 Thr Ser As p A a Ser 205 A a A a Pr a G y Le u Thr lie ValI A a a3y A a 1 Le u As p <210O> 417 <21 1> 611 <212> DNA <213> Cor ynebact er i urn gI ut anm curn <220> <221 CDS <222> (588) <223> FRXA00954 <400> 417 t t cct gt tcgca cct gcg tac aac cct gcg at t gcg cat gt gcag ccg Page 535 seql i st cor r ect ed. t xt Phe Leu Phe Ala Pro Al a Tyr Asn Pro Ala lie Ala His Val G n Pro 1 5 10 gtt cgc cag gcg ctg aaa ttc ccc acc atc ttc aac acg ctt gga cca 96 Val Arg G n Ala Leu Lys Phe Pro Thr Ile Phe Asn Thr Leu y Pro 25 ttg ctg tcc ccg gcg cgc ccg gag cgt cag atc atg ggc gtg gcc aat 144 Leu Leu Ser Pro Ala Arg Pro G u Arg n lie IVet y Val Ala Asn 40 gcc aat cat gga cag ctc atc gcc gag gtc ttc cgc gag ttg ggc cgt 192 Ala Asn Hi s G y n Leu Ile Ala G u Val Phe Arg G u Leu G y Arg 55 aca cgc gcg ctt gtt gtg cat ggc gca ggc acc gat gag atc gca gtc 240 Thr Arg Ala Leu Val Val His G y Ala G y Thr Asp G u IIe Ala Val 70 75 cac ggc acc acc ttg gtg tgg gag ctt aaa gaa gac ggc acc atc gag 288 His G y Thr Thr Leu Val Trp G u Leu Lys G u Asp G y Thr IIe G u 90 cat tac acc atc gag cct gag gac ctt ggc ctt ggc cgc tac acc ctt 336 His Tyr Thr lie G u Pro G u Asp Leu G y Leu G y Arg Tyr Thr Leu 100 105 110 gag gat ctc gta ggt ggc ctc ggc act gag aac gcc gaa gct atg cgc 384 Su Asp Leu Val y G y Leu y Thr u Asn Ala u Ala IVet Arg 115 120 125 gct act ttc gcg ggc acc ggc cct gat gca cac cgt gat gcg ttg gct 432 Ala Thr Phe Ala Gy Thr G y Pro Asp Ala His Arg Asp Ala Leu Ala 130 135 140 gcg tcc gca ggt gcg atg ttc tac ctc aac ggc gat gtc gac tcc ttg 480 Ala Ser Al a G y Ala IVet Phe Tyr Leu Asn G y Asp Val Asp Ser Leu 145 150 155 160 aaa gat ggt gca caa aag gcg ctt tcc ttg ctt gcc gac ggc acc acc 528 Lys Asp Gy Ala G n Lys Ala Leu Ser Leu Leu Ala Asp y Thr Thr 165 170 175 cag gca tgg ttg gcc aag cac gaa gag atc gat tac tca gaa aag gag 576 Sn Ala Trp Leu Ala Lys His u u lie Asp Tyr Ser u Lys u 180 185 190 tct tcc aat gac tagtaataat ctgcccacag tgt 611 Ser Ser Asn Asp 195 <210> 418 <211> 196 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 418 Phe Leu Phe Ala Pro Al a Tyr Asn Pro Ala lie Ala His Val G n Pro 1 5 10 Val Arg G n Ala Leu Lys Phe Pro Thr Ile Phe Asn Thr Leu y Pro 25 Leu Leu Ser Pro Ala Arg Pro G u Arg n lie IVet y Val Ala Asn 40 Ala Asn Hi s G y n Leu lie Ala G u Val Phe Arg G u Leu G y Arg Page 536 Thr Hi s Hi s G u Al a Al a 145 Lys G n Ser Al a Thr Thr Leu 115 Phe Al a Gy Tr p Asn 195 Val Leu G u Gy Gy Al a G n 165 Al a Hi s Tr p G u Leu Gy 135 Phe Al a Hi s seql i st cor r ect ed. t xt Sy Al a y Thr Asp u 75 G u Leu Lys G u Asp y 90 Asp Leu y Leu y Arg 105 G y Thr G u Asn Al a G u 120 125 Pro Asp Ala His Arg Asp 140 Tyr Leu Asn G y Asp Val 155 Leu Ser Leu Leu Ala Asp 170 G u G u lie Asp Tyr Ser 185 SIle Thr Tyr 110 Al a Al a Asp Gy G u 190 Al a I e Thr bet Leu Ser Thr 175 Lys Val G u Leu Ar g Al a Leu 160 Thr G u <210> 419 <211> 1677 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1654) <223> RXN00957 <400> 419 aaccggcaag ccctggatcg aatgaagctc gca aaggcttcag ccccacaatg atttcctcgg tag cat Hi s ttt Phe agc Ser aag Lys ccg Pr o ctt ttc Phe cac Hi s gat Asp tcg Ser acg Thr cag ct a Leu ggt Gy acc Thr cgc Ar g tcg Ser aac gt c Val aca Thr aag Lys acg Thr agg Ar g gca cgc Ar g acc Thr aat Asn 45 tgc Cys gca Al a gag t at Tyr gca Al a ggt Gy acg Thr gt g Val aac igcgagta attatttgat gtttcccaga igtgcccc atg agc acg aat ccc IVet Ser Thr Asn Pro 1 cac gag gat gct tct gca ttg His G u Asp Ala Ser Ala Leu 15 gat gat gca gcc ctg ttg gaa Asp Asp Ala Ala Leu Leu G u att tct tcc ctc gcg gtg ttg lie Ser Ser Leu Ala Val Leu ggc aac acg gtg gta acg cag Sy Asn Thr Val Val Thr G n gtt gcg cgc cta acg cag cag Val Ala Arg Leu Thr n G n 80 acc ttt agc ttc ccc gcc tca Page 537 seql i st cor r ect ed. t xt Leu Gy n Tyr Asn Thr Ala G u Asn Thr Phe Ser Phe 95 Pro Al a Ser 100 gat Asp gt g Val cca Pr o acg Thr 150 ttc Phe gcc Al a ct c Leu cac Hi s gct Al a 230 gaa G u ttc Phe gcc Al a ggc Gy ttc Phe 310 cgc Ar g at c SIle gtt Val cgc Ar g 120 ct c Leu ccc Pr o ct c Leu ct c Leu aag Lys 200 t ac Tyr at t l Ie at t SIle gca Al a aac Asn 280 t cc Ser gct Al a cgt Ar g aat Asn gag G u ttg Leu ggc Gy gt c Val gaa G u 170 ggc Gy t ca Ser acc Thr gat Asp aac Asn 250 tgt Cys tcg Ser gaa G u aac Asn ct c Leu 330 ttg Leu cgc Ar g cag G n ggt G y gag G u 155 at c SIle gt c Val tt g Leu acc Thr gct Al a 235 ggt G y cct Pr o ccg Pr o ctt Leu cgt Ar g 315 aac Asn gat Asp gag G u ttc Phe ttc Phe 140 gag G u gt c Val t cc Ser ctt Leu cct Pr o 220 cag G n gac Asp gat Asp t ac Tyr ttt Phe 300 gag G u cca Pr o at g let ct c Leu 110 t cc Ser ttt Phe gt c Val gac Asp gcc Al a 190 gac Asp gac Asp cgc Ar g t ac Tyr ttc Phe 270 ttc Phe gca Al a cag G n ggc G y act Thr 350 acc Thr ggt G y gat Asp aac Asn at c SIle 175 cca Pr o gcc Al a ggc G y acc Thr caa G n 255 gct Al a t at Tyr t cc Ser ct g Leu t cc Ser 335 gat Asp gca Al a t ac Tyr ttc Phe act Thr 160 aat Asn ggc Gy gcc Al a gac Asp cag G n 240 gtt Val gct Al a at c SIle cct Pr o t ac Tyr 320 at c SIle gcc Al a cca Pr o agc Ser tta Leu 145 t ac Tyr cac Hi s gag G u ct c Leu act Thr 225 at c SIle gt c Val t at Tyr cgt Ar g gag G u 305 cca Pr o aac Asn aaa Lys agc Ser gac Asp 130 gaa G u ccc Pr o cag G n ct c Leu ccc Pr o 210 ctt Leu aat Asn ccg Pr o ct g Leu ggc G y 290 t cc Ser at c SIle gat Asp gag G u acc Thr 115 gcg Al a acc Thr gat Asp gac Asp gag G u 195 gca Al a cgc Ar g gag G u gcg Al a cag G n 275 ct c Leu aac Asn gca Al a gag G u at c lile 355 at c SIle t cc Ser ttt Phe t ac Tyr cag G n 180 gcc Al a acc Thr gtt Val ct g Leu cgc Ar g 260 ct g Leu aac Asn ct c Leu ggt G y ct a Leu 340 gcg Al a gaa G u ct g Leu gaa G u cag G n 165 acc Thr gag G u gaa G u gt g Val aaa Lys 245 act Thr cgt Ar g gaa G u aag Lys acc Thr 325 gat Asp gag G u 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 cac acc atg ctt gtc gat ctc gcc cgc aac gac ctg gcc cgc gtc tcg Page 538 Hi s Thr l'bt Leu Val Asp Leu Ala 360 365 eqi i st car r ect ed. t xt Arg Asn Asp Leu A 37 a Ar g Val Ser 0 gt c ValI t cc Ser 390 gag O31u t tg Le u g aa O31u G31y c ag O31n 470 t ct Ser gt g ValI cga Ar g gcg A a gt g ValI g at As p ggc G31y cgc Ar g 440 ggc G31y ggt G31y cct Pr o aat As n cgc Ar g c ac Hi s tt g Le u 410 ccg Pr o cgt Ar g at g vb t gct A a t ct Ser 490 at t I Ie cgg Ar g t t g Le u 395 gac As p aag Lys5 ggt G31y g at As p gct A a 475 gaa O31u gcg A a gt t ValI 380 gt g ValI gcc A a tt g Le u t ct Ser aat As n 460 gt g ValI gcc A a ct t Le u gcg A a t cc Ser t at Tyr cgc Ar g t at Tyr 445 t gc cys c ag O31n g at As p gct A a g at As p cgt Ar g cgg Ar g gct A a 430 ggt G31y at t I Ie gct A a gag O31u gct A a 510 tt g Le u ac g Thr 400 t gc cys gag O31u gca A a at t I Ie gct A a 480 tt g Le u t cc Ser c ag O31n 385 gcg A a at g vb t ct g Le u gt g ValI cgt Ar g 465 ggt G31y c ac Hi s act Thr gt g ValI ac g Thr aat As n tt g Le u ggg G31y 450 t cg Ser gt g ValI aag Lys5 tt g Le u g at As p tt g Le u at g vb t cgc Ar g 435 t ac Tyr gcg A a gt c ValI gcg A a gag O31u 515 cgc Ar g g ac As p ggc G31y 420 ggc G31y ct g Le u tt t Ph e cgc Ar g t at Tyr 500 gt c ValI 1267 1315 1363 1411 1459 1 507 1 555 1 603 1651 1 677 t gacacacgt t gt tctcat t gat <210O> 420 <211> 518 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 420 l'bt Ser Thr Asn Pro Hi s Val Phe Ser 1 5 As p A a Se r A a Leu Phe A a Hi s Leu 25 A a A a Leu Leu 03 u Se r A a Asp I I e 40 Ser Leu A a Val Leu Lys Ser Ser Val Thr Val Val Thr 03 n Pro Leu Thr As p 70 Ar g Leu Thr O31n O31n Leu G31y O31n Ty r Leu Asp 10 G31YG1Y- Thr Thr I Ar g I Ie Ser 03 y Asn Thr 90 Page 539 Ar g Thr As n cys A a (31u Tyr A a G31y Thr ValI As n Hi s As p I Ie G31y ValI Th r Se r Ph e Pr o Ala Se r As p Ala 1 00 seqI i st car r ect ed. t xt Val AspOuArg OuArg 1 05 Leu Thr A a 110 Pr o Ser Le u 145 Tyr Hi s O31u Le u Thr 225 li1e ValI Tyr Ar g O31u 305 Pr o As n Ly s Le u O31n 385 A a vb t Le u ValI Thr 115 A a Thr As p As p O31u 1 95 A a Ar g O31u A a O31n 275 Le u As n A a Ou le 355 Ar g As p Le u vb t Ar g 435 Tyr O31u Le u O31u O31n 1 65 Thr O31u O31u ValI Ly s 245 Thr Ar g O31u Ly s Thr 325 As p O31u Ser Tyr Pr o 405 Thr ValI Ar g ValI Pr o Thr 150 Ph e A a Le u Hi s A a 230 O31u Ph e A a G31y Ph e 310 Ar g I Ie Hi s ValI Ser 390 O31u Le u O31u (31y Le u Le u 135 Le u ValI Ly s As n A a 215 As p As n Thr Thr Ar g 295 Thr Pr a Ar g Thr Pr a 375 Ar g Le u Thr Ly s As n 455 Ar g 120 Le u Pr a Le u Le u Ly s 200 Tyr I Ie I Ie A a As n 280 Ser A a Ar g As n vb t 360 A a ValI As p G31y Ar g 440 (31y Ly s vb t A a A a A a 1 85 Le u O31n Pr a Tyr Pr a 265 Pr a Tyr A a O31y O31u 345 Le u Ser vb t A a A a 425 Ar g As p O31n O31y O31u 1 55 li1e ValI Le u Thr A a 235 O31y Pr a Pr a Le u Ar g 315 As n As p As p Ar g Le u 395 As p Ly s O31y Ph e Ph e 140 O31u ValI Ser Le u Pr a 220 O31n As p As p Tyr Ph e 300 O31u Pr a vb t Le u ValI 380 ValI A a Le u Ser O31u 125 A a Ser Le u As n I Ie 205 Hi s Ph e I Ie A a vb t 285 O31y Le u As p Ar g A a 365 A a Ser Tyr Ar g Tyr 445 Ser Ph e ValI As p A a 1 As p As p Ar g Tyr Ph e 270 Ph e A a O31n O31y Thr 350 Ar g As p Ar g Ar g A a 430 O31y O31y As p As n I Ie 1 Pr a A a O31y Thr O31n 255 A a Tyr Ser Le u Ser 335 As p As n Le u ValI A a 415 vb t O31y Tyr Ph e Thr 1 As n O31y A a As p O31n 240 ValI A a I Ie Pr a Tyr 320 li1e A a As p Le u Thr 400 cys O31u A a N/t Asp Asn Cys lieVal Ilie 460 Page 540 Arg Ser Al a Phe Val 465 G31y ValI Val Ar g As p 485 Hi s Lys Al a Ty r Al a 500 Thr Leu O31u Val I Ie 515 seqI i st car r ect ed. t xt O31n As p G31y ValI Ala Ala Val On Ala Oy Ala 470 475 480 Ser As n Pro O31n Ser O31u Al a As p O31u Th r Leu 490 495 Val Leu Asn Ala Ilie Al a Leu Al a Al a G31y Ser 505 510 Ar g <210O> 421 <21 1> 1151 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1128) <223> FRXA00957 <400> 421 gat t tc tt a Asp Phe Leu aac act t ac Asn Thr Tyr at c aat cac Il e Asn Hi s cca ggc gag Pr o 31y O31u gcc gcc ct c Al a Al a Leu ggc gac act O3 y Asp Thr acc cag at c Thr 03 n Il e caa gt t gt c O31n Val Val 115 gct gct t at Al a Al a Tyr 130 t at at c cgt Tyr Ilie Ar g 145 t cc cct gag Ser Pr o (3u ac c Thr g at As p g ac As p gag O31u gca Al a cgc Ar g gag O31u gcg Al a c ag O31n ct c Le u aac As n 1 65 g aa O31u c ag O31n ac c Thr gag O31u 55 g aa O31u gt g ValI aaa Ly s act Thr cgt Ar g 135 g aa O31u aag Ly s ac g Thr tt c Ph e gcc Al a 40 ct c Le u c ac Hi s gct Al a g aa O31u tt c Ph e 120 gcc Al a ggc O31y Ph e ct c Le u gt c ValI aaa Ly s aac As n gcc Al a g at As p aac As n 1 ac c Thr ac c Thr cgc Ar g ac c Thr gct Al a gcg Al a gcc Al a ct t Le u c aa O31n ccc Pr o t ac Tyr cca Pr o ccg Pr o t at Tyr 1 gct Al a gt c ValI g aa O31u O31y t ca Ser ac c Thr g at As p aac As n t gt cys t cg Ser 140 g aa O31u aac As n gag O31u at c I Ie gt c ValI tt g Le u ac c Thr gct Al a ggt O31y cct Pr o 125 ccg Pr a ct t Le u cgt Ar g ag c Ser ct g Le u aac As n at c I Ie c ac Hi s tt c Ph e at c I Ie gca Al a at g O31y ct g Le u 1 Page 541 seql i st cor r ect ed. t xt ctg tac cca atc gca ggt acc cgc Leu Tyr Pro lie Ala G y Thr Arg 180 tcc atc aac gat gag cta gat atc Ser Ile Asn Asp u Leu Asp Ile 195 200 gat gcc aaa gag atc gcg gag cac Asp Ala Lys u Ile Ala Gu His 210 215 aac gac ctg gcc cgc gtc tcg gtc Asn Asp Leu Ala Arg Val Ser Val 225 230 ctt ttg cag gtg gat cgc tat tcc Leu Leu G n Val Asp Arg Tyr Ser 245 gtg acg gcg acg ttg gac cca gag Val Thr Ala Thr Leu Asp Pro G u 260 gcg tgc atg aat atg ggc acg ttg Al a Cys IVet Asn IVet G y Thr Leu 275 280 atg gag ctg ttg cgc ggc gtc gaa IVet G u Leu Leu Arg G y Val G u 290 295 ggg gca gtg ggg tac ctg cgc ggc Sy Al a Val y Tyr Leu Arg y 305 310 gtt att cgt tcg gcg ttt gtc cag Val Ile Arg Ser Ala Phe Val G n 325 ggt gct ggt gtg gtc cgc gat tct Sy Ala y Val Val Arg Asp Ser 340 acg ttg cac aag gcg tat gcc gtg Thr Leu His Lys Ala Tyr Ala Val 355 360 ggt tcc act ttg gag gtc atc cga G y Ser Thr Leu G u Val IIe Arg 370 375 <210> 422 <211> 376 <212> PRT <213> Cor ynebact eri um gl ut anm cur <400> 422 Asp Phe Leu G u Thr Phe G u Thr 1 5 Asn Thr Tyr Pro Asp Tyr n Phe Ile Asn His n Asp n Thr Ala 40 ccc cgt gga ctc aac cca Pro Arg Gy Leu 185 cgc aat gag ttg Arg Asn G u Leu acc atg ctt gtc Thr bet Leu Val 220 cca gcg tcg cgc Pro Ala Ser Arg 235 cgc gtg atg cac Arg Val Met His 250 ctt gat gct ttg Leu Asp Ala Leu 265 acc ggc gct ccg Thr Gy Ala Pro aag cgc agg cgt Lys Arg Arg Arg 300 aat ggc gat at g Asn y Asp IVet 315 gat ggt gtg gct Asp y Val Al a 330 aat cct caa t ct Asn Pro G n Ser 345 ttg aat gcc att Leu Asn Ala lle Asn Pro 190 gat at g Asp IVet 205 gat ct c Asp Leu cgg gtt Arg Val ttg gtg Leu Val gac gcc Asp Al a 270 aag ttg Lys Leu 285 ggt tct G y Ser gat aat Asp Asn gct gtg Al a Val gaa gcc G u Al a 350 gcg ctt Al a Leu gat Asp cgc Ar g gcc Al a gcg Al a t cc Ser 255 t at Tyr cgc Ar g t at Tyr tgc Cys cag G n 335 gat Asp gct Al a 576 624 672 720 768 816 864 912 960 1008 1056 1104 1151 tgacacacgt tgttctcatt gat n Leu Pro Ala Val u u G uSer Val 10 Val Leu Ala u Ile Val Leu Asp 25 Lys Leu Ala G y Val Ser Asn Ala Page 542 seql i st cor r ect ed. t xt Pro Gy G u Leu G u Al a u Leu Asn Lys Leu Ser Leu Leu Ile Asp 55 Ala Ala Leu Pro Ala Thr G u His Ala Tyr G n Thr Thr Pro His Asp 70 75 Sy Asp Thr Leu Arg Val Val Ala Asp lie Pro Asp Ala G n Phe Arg 90 Thr G n I e Asn G u Leu Lys G u Asn I e Tyr Asn G y Asp I e Tyr 100 105 110 G n Val Val Pro Ala Arg Thr Phe Thr Ala Pro Cys Pro Asp Ala Phe 115 120 125 Ala Ala Tyr Leu G n Leu Arg Ala Thr Asn Pro Ser Pro Tyr IVet Phe 130 135 140 Tyr lie Arg y Leu Asn G u y Arg Ser Tyr G u Leu Phe y Ala 145 150 155 160 Ser Pro u Ser Asn Leu Lys Phe Thr Ala Ala Asn Arg G u Leu G n 165 170 175 Leu Tyr Pro Ile Al a G y Thr Arg Pro Arg G y Leu Asn Pro Asp G y 180 185 190 Ser Ile Asn Asp G u Leu Asp lie Arg Asn G u Leu Asp IVet Arg Thr 195 200 205 Asp Ala Lys G u Ile Al a G u His Thr IVet Leu Val Asp Leu Ala Arg 210 215 220 Asn Asp Leu Ala Arg Val Ser Val Pro Ala Ser Arg Arg Val Ala Asp 225 230 235 240 Leu Leu G n Val Asp Arg Tyr Ser Arg Val IVet His Leu Val Ser Arg 245 250 255 Val Thr Ala Thr Leu Asp Pro G u Leu Asp Ala Leu Asp Ala Tyr Arg 260 265 270 Al a Cys IVet Asn IVet G y Thr Leu Thr G y Al a Pro Lys Leu Arg Al a 275 280 285 IVet G u Leu Leu Arg G y Val G u Lys Arg Arg Arg y Ser Tyr y 290 295 300 Sy Al a Val y Tyr Leu Ar g y Asn y Asp Iet Asp Asn Cys Ile 305 310 315 320 Val Ile Arg Ser Ala Phe Val G n Asp y Val Ala Ala Val G n Ala 325 330 335 Sy Ala y Val Val Arg Asp Ser Asn Pro n Ser u Ala Asp u 340 345 350 Thr Leu His Lys Ala Tyr Ala Val Leu Asn Ala lie Ala Leu Ala Ala 355 360 365 G y Ser Thr Leu G u Val IIe Arg 370 375 <210> 423 <211> 1068 <212> DNA Page 543 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1045) <223> RXA02687 <400> 423 caaaaccatg ctcggcgggg gagagttggg ccaagcggtt cgctaattga gcaacagaca tggacaccca cct t agttcg gcgggt t aag ctgtgtaacc act gtt gtg gcc tat ttg ggg cct gcc gga acc Thr Val Val Ala Tyr Leu y Pro Ala y Thr 15 ctc tac aaa ttt gcc gac gcc ggc gta ttc ggc Leu Tyr Lys Phe Ala Asp Ala y Val Phe y 30 cag cta cca gcc aaa tcg cca caa gaa gct gtc G n Leu Pro Ala Lys Ser Pro n G u Ala Val 45 ggc acc gcc cag ttc gcg gtg gtc gcc atc gaa Sy Thr Ala n Phe Ala Val Val Ala Ile u 60 ccc gtc acc ccc acc ttc gac gcc ctt gac cag Pro Val Thr Pro Thr Phe Asp Ala Leu Asp G n 75 80 atc atc gcc gaa gaa gaa ctc gac atc gcc ttt Ile Ile Ala G u G u G u Leu Asp Ile Ala Phe 95 cca ggg act tcg ctt gcc gac gtc aaa acc ctc Pro G y Thr Ser Leu Al a Asp Val Lys Thr Leu 105 110 ggg tac caa caa gtg aaa aac tgg atg gca acc Sy Tyr n G n Val Lys Asn Trp IVet Ala Thr 120 125 atg tat ctt tca gca agc tcc aac ggc gcc ggc IVet Tyr Leu Ser Al a Ser Ser Asn G y Al a G y 135 140 gaa gga acc gcc gac gca gcc gca gcg ccc tcc G u y Thr Ala Asp Al a Ala Ala Ala Pro Ser 150 155 160 ttc gga ctg gaa cgc ctt gtt gat gat gtc gcc Phe y Leu G u Arg Leu Val Asp Asp Val Ala 170 175 cgc acc cgc ttc gtt gct gtc caa gcc caa gca Arg Thr Arg Phe Val Al a Val G n Ala G n Ala 185 190 acc ggc cac gac cgc acc tcc gtc att ttc tcc Thr G y His Asp Arg Thr Ser Val lie Phe Ser 200 205 ggc agc ctc gtg cgc gcc ctc aac gaa ttc gcc Sv Ser Leu Val Ara Al a Leu Asn G u Phe Ala 1 ttc Phe gac Asp gac Asp aac Asn ggc G y t cc Ser gcc Al a acc Thr gca Al a 145 cgc Ar g gac Asp gcc Al a ct a Leu acc Thr ggt Sy gcc Al a ttc Phe t cc Ser at c SIle acc Thr at t SIle 130 caa G n gca Al a gt c Val gtt Val ccg Pr o 210 gaa G u gag G u gt c Val gt c Val aac Asn at g Met cac Hi s 115 ccg Pr o at g Met gcc Al a cgt Ar g t cc Ser 195 aat Asn gaa G u at c SIle cgc Ar g gac Asp gt g Val gt c Val 100 ccg Pr o gac Asp gtt Val gaa G u ggc Sy 180 gaa G u gt g Val gcc Al a gag G u cac Hi s ggc G y caa G n cgg Ar g gtt Val gcc Al a gcc Al a ct c Leu 165 gcc Al a ccg Pr o cca Pr o atg agc gac gca cca IVet Ser Asp Ala Pro atc cgc ggc gtt gac lie Arg y Val Asp J Page 544 seql i st cor r ect ed. t xt 215 220 225 ctc acc cgc atc gaa tcc cgc ccc acc cgc aaa gtc ttc Leu Thr Arg lie u Ser Arg Pro Thr Arg Lys Val Phe 230 235 240 cgc ttc cac ctg gac ata tcc gga cat atc cgc gat atc Arg Phe His Leu Asp IIe Ser G y His lie Arg Asp IIe 250 255 gaa gcc ctc cgc gca ctc cac ctc caa gcc gaa gaa ctc G u Ala Leu Arg Ala Leu His Leu n Ala u G u Leu 265 270 ggc tcc tgg ccc tcc aac cgt gcg gaa gac agc acg ccc Sy Ser Trp Pro Ser Asn Arg Ala G u Asp Ser Thr Pro 280 285 290 caa cta gct aag cta cac aag gcg gac gaa tgg gtt cgc G n Leu Ala Lys Leu His Lys Ala Asp G u Trp Val Arg 295 300 305 gaa gga agg aaa ctt aac tagccatggc cggccggatt att G u y Arg Lys Leu Asn 310 315 <210> 424 <211> 315 <212> PRT <213> Corynebacter i um gl ut ai cum gga G y ccc Pr o gt c Val 275 caa G n gca Al a acc Thr gt c Val 260 ttc Phe acc Thr gca Al a 835 883 931 979 1027 1068 <400> 424 IVet Ser Asp Ala Pro Thr Val 1 Phe Asp Asp Asn Gy Ser Al a Thr Al a 145 Ar g Asp G u G u Val Val Asn bet Hi s 115 Pr o Met Al a Ar g 5 Al a G u Hi s Gy G n Ar g Val Al a Al a Leu 165 Al a Tyr Leu Thr Val SIle Sy Tyr Tyr 135 Sy Sy Thr Val Al a Tyr 10 Lys Phe Al a Pro Al a Lys 40 Al a G n Phe Thr Pro Thr Al a G u G u 90 Thr Ser Leu 105 Sn G n Val 120 Leu Ser Al a Thr Al a Asp Leu G u Arg 170 Arg Phe Val 185 Leu Asp Ser Al a Phe 75 G u Al a Lys Ser Al a 155 Leu Sy Al a Pr o Val Asp Leu Asp Asn Ser 140 Al a Val Pr o Sy G n Val Al a Asp Val Tr p 125 Asn Al a Asp Al a Val G u Al a Leu SIle Lys 110 Met Sy Al a Asp Sy Phe Al a l Ie Asp Al a Thr Al a Al a Pr o Val 175 Thr Sy Val G u G n Phe Leu Thr Sy Ser 160 Al a Ala Val n Ala n Ala 190 Page 545 seql i st cor r ec Ala Val Ser G u Pro Thr y His Asp Arg Thr 195 200 Leu Pro Asn Val Pro G y Ser Leu Val Arg Ala 210 215 lie Arg y Val Asp Leu Thr Arg lie u Ser 225 230 235 Val Phe G y Thr Tyr Arg Phe His Leu Asp lie 245 250 Asp Ile Pro Val Ala G u Ala Leu Arg Ala Leu 260 265 G u Leu Val Phe Val G y Ser Trp Pro Ser Asn 275 280 Thr Pro G n Thr Asp G n Leu Ala Lys Leu His 290 295 Val Arg Ala Ala Ser G u y Arg Lys Leu Asn 305 310 315 <210> 425 <211> 1353 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1330) <223> RXN01698 <400> 425 cgctagtgcc tgcagttatg tcgctttttg acgtcgaaaa gggtgggctg gatacgtctt ctgcggcctt tcgtgcgat a ted. txt Ser Val 205 Leu Asn 220 Arg Pro Ser G y Hi s Leu Arg Ala 285 Lys Al a 300 Phe Phe Ar g SIe 255 Al a Asp G u gcaat t t t aa aaagccct t g atg cta ggc atg ctt 11! IVet Leu Gy IVet Leu cga Ar g gtt Val t at Tyr aag Lys ct c Leu gat Asp act Thr cac Hi s ttg Leu gag G u ttg Leu tgg Tr p gca Al a cca Pr o cgc Ar g gac Asp agc Ser acc Thr ggt G y gca Al a cga Ar g gcg Al a ccc Pr o 75 at c SIle gaa G u ggc Gy cgc Ar g ttg Leu at c SIle at g Met cac Hi s ccc Pr o gga Gy ttc Phe at c SIle tct Ser cag G n act Thr ggt Gy acc Thr at c SIle gct Al a 1 gcg Al a aaa Lys cgc Ar g ggc Gy ggc Gy ttg Leu ctt Leu gat Asp ggc Gy at c SIle aac Asn gac Asp at c SIle gag G u gct Al a cgc Ar g act Thr at g abt gcc Al a gtc Val cgc Ar g cac Hi s gag G u gaa 100 cca gat aac gtt gcg gcg atg tct tcg ggt cgg ggc gca aaa ctg act Page 546 seql i st cor r ect ed. t xt Pro Asp Asn Val Ala Al a MIt Ser Ser y Arg y Al a Lys Leu Thr 115 105 cgt Ar g ttc Phe gca Al a 150 acc Thr gag G u gat Asp at c SIle at c SIle 230 at t SIle ggc Gy gct Al a aac Asn at g Met 310 tct Ser gca Al a gcc Al a cgt Ar g 120 gat Asp cgc Ar g ggc Gy t ac Tyr t cc Ser 200 gaa G u gaa G u ggc G y cag Gn cga Ar g 280 gt a Val aac Asn gt g Val acc Thr gt c Val 360 ggc G y cgc Ar g gca Al a gaa Gu 170 ggc Gy gtt Val gag G u at t SIle gat Asp 250 at c SIle ggc G y cgc Ar g gaa Gu cgc Ar g 330 at c SIle gca Al a cac Hi s aac Asn gca Al a 155 gt g Val gcg Al a cgt Ar g gcc Al a gtt Val 235 cgc Ar g aag Lys t cc Ser aac Asn acc Thr 315 gcc Al a cac Hi s gaa Gu gct Al a gt g Val 140 gca Al a ctt Leu gag Gu gca Al a gca Al a 220 gaa Gu ct c Leu ggc Gy gaa Gu acc Thr 300 ct g Leu ct g Leu cag G n gca Al a gat Asp 125 ct g Leu acc Thr t cc Ser cca Pr o ttc Phe 205 aag Lys ggc G y gat Asp gt g Val gcc Al a 285 aac Asn cgc Ar g aaa Lys cgt Ar g at g let 365 110 t ac Tyr gag G u gtt Val cac Hi s acc Thr 190 ggt Gy aaa Lys ct g Leu gcg Al a gaa Gu 270 cac Hi s cgt Ar g gtt Val acc Thr t cc Ser 350 gt c Val gca Al a cgt Ar g gcg Al a gt a Val 175 ttt Phe aaa Lys gcc Al a ccc Pr o cag G n 255 at c SIle gat Asp gca Al a cgt Ar g at t SIle 335 gac Asp acc Thr ggc G y tct Ser cgt Ar g 160 at t SIle gca Al a gac Asp ggc G y at c SIle 240 at c SIle ggt Gy gaa G u ggt Gy gct Al a 320 gat Asp gt g Val ct g Leu at g let t ca Ser 145 t cc Ser t cc Ser gat Asp gct Al a gat Asp 225 ggt G y gca Al a gac Asp gt g Val ggc Gy 305 ggc Gy at g let tgc Cys gtt Val ct c Leu 130 gcc Al a ttc Phe at t SIle at t SIle gaa G u 210 acc Thr ttg Leu gct Al a ggt Gy ttc Phe 290 ct c Leu at g Met gaa G u gct Al a ct c Leu 370 aag Lys cgt Ar g ct g Leu ggt Gy caa G n 195 gaa G u ct c Leu ggc G y gca Al a ttc Phe 275 ct g Leu gaa G u aag Lys aac Asn gtt Val 355 gcc Al a t ac Tyr gag G u cgt Ar g gcg Al a 180 gca Al a t cc Ser ggt G y t ca Ser ct c Leu 260 gaa Gu gat Asp ggc Gy cca Pr o ggc Gy 340 cca Pr o cgc Ar g gga G y acg Thr gaa G u 165 t cc Ser at c SIe at g let ggc G y cac Hi s 245 at g let gaa Gu gac Asp ggc Gy at t SIe 325 aag Lys gcc Al a gca Al a 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 gtc ctg cag aaa ttc ggc ggt gac tcc ctg agc gaa acc aag agc aac Page 547 Val Leu O31n 375 att gac acc Il e Asp Thr 390 t ta gag gat Leu 03 u Asp Ph e ct c Le u gcg A a 410 seqI i st car r ect ed. t xt G31y G31y Asp Ser Leu Ser O31u Thr Lys Ser Asn 380 385 aaa aac att gag gaa cga at g aaa t tc gaa ggt Lys Asn I Ie 31u O31u Ar g I'bt Lys Phe O31u G31y 395 400 405 t aat gaagt g aat gat caaa tt c 1315 1353 <210O> 426 <211> 410 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 426 I'b t L eu 31y I'b t Leu Ar g Tr p Thr A a Ly s Ar g G31y G31y Le u G31y vb t Ser 145 Ser Ser As p A a As p 225 G31y A a I Ie O31u A a Ar g Thr Nibt Ly s 115 Ly s Ar g Le u G31y O31n 1 95 O31u Le u G31y A a Thr Ser G31y Tr p As p Thr G31y Thr O31u 1 65 Ser G31y Hi s 245 O31u O31n Ph e Thr Ly s As p Pr a As p 135 A a Le u Pr a As p A a 215 ValI Ser I Ie Th r A a 10 I'b t Pr o Ala Ar g O31n As p O3 y Ser Thr Thr 90 Val Ala 1 05 Pro O31y Ala Ar g Val Ala Val O31u 1 70 Thr 03 y 1 85 Pr o Val I Ie O31u Val I Ie 31u As p 250 A a I I e O31y A a Ar g A a Pr a li1e A a Hi s As n A a 1 ValI A a Ar g A a ValI 235 Ar g O31u O31y Ar g Le u li1e vb t vb t A a ValI 140 A a Le u O31u A a A a 220 O31u Le u Ser ValI Le u Thr Ser Ser Ser As p 125 Le u Thr Ser Pr a Ph e 205 Ly s O31y As p Hi s Pr a O31y Ph e I Ie Ser Ser 110 Tyr O31u ValI Hi s Thr 1 O31y Ly s Le u A a O31y ValI Tyr Le u vb t As p O31y A a Ar g A a ValI 1 Ph e Ly s A a Pr a O31n 255 265 Lys Oy Val Gu Ilie 3y 270 Page 548 seql i st cor r ect ed. t xt Asp G y Phe G u G u Ala Arg Arg Arg G y Ser u Ala His Asp u 275 280 285 Val Phe Leu Asp Asp Asn y Val Tyr Arg Asn Thr Asn Arg Ala y 290 295 300 Sy Leu G u G y y Iet Thr Asn G y u Thr Leu Arg Val Arg Ala 305 310 315 320 G y IVet Lys Pro Ile Ser Thr Val Pro Arg Ala Leu Lys Thr Ile Asp 325 330 335 IVet G u Asn G y Lys Ala Ala Thr G y lie His n Arg Ser Asp Val 340 345 350 Cys Al a Val Pro Al a Al a G y Val Val Al a u Al a Met Val Thr Leu 355 360 365 Val Leu Ala Arg Ala Val Leu G n Lys Phe Gy y Asp Ser Leu Ser 370 375 380 G u Thr Lys Ser Asn Ile Asp Thr Tyr Leu Lys Asn lie G u G u Arg 385 390 395 400 IVet Lys Phe G u G y Leu G u Asp G y Al a 405 410 <210> 427 <211> 1013 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA01698 <400> 427 ggc aac act gag tgg gat aag tgg acc acc atc atg tcc tct gac gct 48 G y Asn Thr G u Trp Asp Lys Trp Thr Thr I e IVet Ser Ser Asp Al a 1 5 10 ttg gac atg gaa gac cca gat aac gtt gcg gcg atg tct tcg ggt cgg 96 Leu Asp IVet G u Asp Pro Asp Asn Val Ala Ala IVet Ser Ser y Arg 25 ggc gca aaa ctg act cgt ccg cgt cca ggc cac gct gat tac gca ggc 144 G y Ala Lys Leu Thr Arg Pro Arg Pro G y His Ala Asp Tyr Ala G y 40 atg ctc aag tac gga ttc gat gat gcc cgc aac gtg ctg gag cgt tct 192 IVet Leu Lys Tyr G y Phe Asp Asp Al a Arg Asn Val Leu G u Arg Ser 55 tca gcc cgt gag acg gca gca cgc gtg gca gca gca acc gtt gcg cgt 240 Ser Ala Arg G u Thr Al a Ala Arg Val Ala Ala Ala Thr Val Ala Arg 70 75 tcc ttc ctg cgt gaa acc ttg ggc gtg gaa gtg ctt tcc cac gta att 288 Ser Phe Leu Arg G u Thr Leu y Val G u Val Leu Ser His Val lie 90 tcc att ggt gcg tcc gag cct tac act ggc gcg gag cca acc ttt gca 336 Ser lie Gy Ala Ser G u Pro Tyr Thr Gy Ala G u Pro Thr Phe Ala 100 105 110 Page 549 seql i st cor r ect ed. t xt gat att caa gca atc gat gat tcc cca gtt Asp lie On Ala lie Asp Asp Ser Pro Val 115 120 cgt gca Arg Ala ttc ggt aaa gac Phe G y Lys Asp gct Al a gat Asp 145 ggt Gy gca Al a gac Asp gt g Val ggc Gy 225 ggc Gy at g Met tgc Cys gtt Val gaa G u 305 at g Met gaa gaa tcc G u u Ser 130 acc ctc ggt Thr Leu G y ttg ggc tca Leu G y Ser gct gca ct c Al a Al a Leu 180 ggt ttc gaa G y Phe G u 195 ttc ctg gat Phe Leu Asp 210 ctc gaa ggc Leu G u G y atg aag cca IVet Lys Pr o gaa aac ggc Su Asn y 260 gct gtt cca Al a Val Pr o 275 ctc gcc cgc Leu Ala Arg 290 acc aag agc Thr Lys Ser aaa ttc gaa Lys Phe G u at g Mt ggc Gy cac Hi s 165 at g Met gaa G u gac Asp ggc Gy at t SIle 245 aag Lys gcc Al a gca Al a aac Asn ggt Gy 325 at c SIle at c SIle 150 at t SIle ggc Gy gct Al a aac Asn at g Met 230 tct Ser gca Al a gcc Al a gt c Val at t SIle 310 gcg Al a 135 gt g Val tct Ser at t SIle cgt Ar g ggc Gy 215 acc Thr act Thr gca Al a ggt Gy ct g Leu 295 gac Asp gaa G u gaa G u ggc Gy cag G n cga Ar g 200 gt a Val aac Asn gt g Val acc Thr gt c Val 280 cag G n acc Thr at c SIle gt g Val gaa G u gcc Al a 185 cgt Ar g t ac Tyr ggt Gy cct Pr o gga Gy 265 gtt Val aaa Lys t ac Tyr gag G u at t SIle gat Asp 170 at c l Ie ggc Gy cgc Ar g gaa G u cgc Ar g 250 at c SIle gca Al a ttc Phe ct c Leu gcc Al a gtt Val 155 cgc Ar g aag Lys t cc Ser aac Asn acc Thr 235 gcc Al a cac Hi s gaa G u ggc Gy aaa Lys 315 gca Al a 140 gaa G u ct c Leu ggc Gy gaa G u acc Thr 220 ct g Leu ct g Leu cag G n gca Al a ggt Gy 300 aac Asn 125 aag aaa Lys Lys ggc ctg G y Leu gat gcg Asp Al a gtg gaa Val G u 190 gcc cac Al a Hi s 205 aac cgt Asn Arg cgc gtt Arg Val aaa acc Lys Thr cgt t cc Arg Ser 270 at g gt c Mvt Val 285 gac t cc Asp Ser att gag Ile G u gcc Al a ccc Pr o cag G n 175 at c SIe gat Asp gca Al a cgt Ar g att SIe 255 gac Asp acc Thr ctg Leu gaa G u tta gag gat gga Leu G u Asp G y taatgaagtg aatgatcaaa 1010 1013 ttc <210> 428 <211> 330 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 428 G y Asn Thr G u Trp Asp Lys Trp Thr Thr I e IVet Ser Ser Asp Al a 1 5 10 Page 550 seqi i st car r ect ed. t xt Leu Asp [Vbt (31u Asp Pro Asp Asn Val Ala Ala libt Ser 25 Ser G31y Ar g G31y vb t Ser Ser Ser As p A a As p 145 G31y A a As p ValI G31y 225 G31y vb t cys ValI (31u 305 vb t Ly s Ly s Ar g Le u G31y O31n 115 (31u Le u G31y A a Ph e 1 95 Le u (31u Ly s As n ValI 275 A a Ly s Ph e Thr G31y Thr (31u Ser I Ie G31y Hi s 1 65 vb t (31u As p Gy le 245 Ly s A a A a As n Ar g Ph e A a 70 Thr (31u As p I Ie I Ie 150 li1e G31y A a As n vb t 230 Ser A a A a ValI I Ie 310 Pr o As p A a Le u Pr o As p A a 135 ValI Ser I Ie Ar g G31y 215 Thr Thr A a G31y Le u 295 As p Pr o A a ValI ValI Thr 1 Pr o I Ie ValI (31u A a 1 Ar g Tyr G31y Pr a G31y 265 ValI Ly s Tyr G31y Ar g A a (31u 90 G31y ValI (31u I Ie As p 1 70 li1e G31y Ar g (31u Ar g 250 li1e A a Ph e Le u A a 330 Hi s As n A a ValI A a Ar g A a ValI 1 Ar g Ly s Ser As n Thr 235 A a Hi s (31u G31y Ly s 315 A a ValI A a Le u (31u A a A a 140 (31u Le u G31y O31u Thr 220 Le u Le u O31n A a G31y 300 As n As p Le u Thr Ser Pr a Ph e 125 Ly s G31y As p ValI A a 205 As n Ar g Ly s Ar g vb t 285 As p I Ie A a Ar g A a ValI Ph e Ly s A a Pr a O31n 1 li1e As p A a Ar g I Ie 255 As p Thr Le u O31u O31u (1y 325 Leu 03 u Asp 03 y <210O> 429 <21 1> 906 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS Page 551 seqI i st car r ect ed. t xt <222> (883) <223> RXA01095 <400> 429 gaaaccccag gt caaagct a gggt gt ggca ccct gat t tc ataaccttaa acacagcatt ggttggaagg aggttggggc tttcgccatg tgtgttcggg atg gtt gca aca gag 11! I'bt Val Al a Thr O u aac As n ac g Thr ac g Thr gt c ValI g at As p ct t Le u at c I Ie ccc Pr o g ac As p t ct Ser 1 50 gt g ValI gt g ValI g aa O31u at t I Ie at g vb t ggt G31y gct Al a gt c ValI gt g ValI t at Tyr g ac As p at c I Ie 120 ct t Le u gt g ValI aac As n gca Al a tt t Ph e 200 ct c Le u at g vb t c aa O31n g at As p tt t Ph e t ac Tyr gt g ValI aaa Ly s ccg Pr o tt c Ph e cgc Ar g g aa O31u 1 70 g at As p ggt G31y ggt G31y gaa O31u gaa O31u at t I Ie g at As p gct Al a 75 cgc Ar g t ct Ser t at Tyr ccc Pr o acc Thr 155 gaa O31u at t I Ie at c I Ie t gc Cy s at c I Ie gt c ValI gct Al a cgt Ar g gca Al a ag g Ar g g aa O31u c aa O31n gt a ValI 140 g aa O31u gcg Al a act Thr ac c Thr ag c Ser 220 gct Al a aaa Ly s t ca Ser 45 tt t Ph e c ag O31n gga G31y gt a ValI at c I Ie 125 t gg Tr p t ca Ser cat Hi s ggt G31y c aa O31n 205 ag c Ser gcg Al a act Thr gt c ValI gca Al a gt t ValI c ag O31n g ac As p 110 c ac Hi s gcg Al a tt g Le u cgt Ar g t at Tyr 1 90 tt c Ph e cct Pr o g aa O31u 15 aaa Ly s tt t Ph e t cc Ser gcg Al a tt t Ph e 95 at t I Ie g aa O31u at g vb t ggc O31y gca Al a 1 75 ac c Thr at g vb t aaa Ly s at a I Ie t ct Ser tt t Ph e aat As n ggt O31y 80 g at As p ccc Pr a gcc Al a g aa O31u at g vb t 1 60 gt g ValI O31y ccc Pr a g aa O31u 1 t cg Ser cga Ar g t cg Ser t gg Tr p 65 tt t Ph e ac c Thr at t I Ie cgc Ar g c aa O31n 145 ac a Thr g ac As p t ca Ser aaa Ly s ct c Le u 225 gct Al a t ca Ser t ct Ser 50 t ct Ser ggc O31y gca Al a ct g Le u gt c ValI 130 gct Al a gcc Al a gca Al a ct c Le u gag O31u 210 at g vb t cgg Ar g gca Al a 35 gga O31y g at As p gca Al a gt a ValI ct t Le u 115 at g vb t cga Ar g at c I Ie gga O31y act Thr 1 gt a ValI cgg Ar g g aa O31u 20 ggt O31y t gt cys cat Hi s t ca Ser cgc Ar g 1 00 cat Hi s ggc O31y ct g Le u gt g ValI gcg Al a 1 tt g Le u gcc Al a tt t Ph e gca Al a ct c Le u aat As n t cg Ser at g vb t g at As p g at As p at c I Ie g aa O31u t ct Ser 1 ac a Th r cct Pr a cgc Ar g gca Al a cga cat t ct gca gac gcc at c t tt gt t cca cat gca gac ct c gcc acc Arg His Ser AiaAsp Ala Ilie Phe Val Pro His AiaAsp Leu AiaThr Page 552 seql i st cor r ect ed. t xt 230 235 240 245 aca aaa tct ctt gtg aca gca ggt atg cat cca gcg tgc cca tcg cgt Thr Lys Ser Leu Val Thr Ala G y IVet His Pro Ala Cys Pro Ser Arg 250 255 260 tgaagaggtg ctctgtggtc agc <210> 430 <211> 261 <212> PRT <213> Corynebacter i um gl utam cum <400> 430 Met Val Ala 1 Ser Al a Ar g Arg Ser Ala Ser Ser G y Trp Ser Asp Phe G y Al a Thr Al a Val Ile Leu Leu 115 Arg Val 'bet 130 G n Al a Ar g 145 Thr Ala lle Asp Al a G y Ser Leu Thr 195 Lys G u Val 210 Leu IVet Arg 225 Al a Asp Leu Al a Cys Pr o Thr G u 5 G u Al a G y Leu Cys Asn Hi s Ser Ser 'bet Arg Asp 100 His Asp G y lle Leu G u Val Ser 165 Al a Thr 180 Leu Pro Al a Ar g Phe Al a Al a Thr 245 Ser Arg 260 Asn Arg IVet Leu Al a Val 55 Hi s Al a Ar g SIle Al a 135 Leu Ar g Val Al a Val 215 Hi s Sy Al a 40 Val Val Tyr Asp SIle 120 Leu Val Asn Al a Phe 200 Leu Ser Leu IVet 10 Phe G n 25 Phe Asp Al a Phe Asp Tyr Thr Val 90 I e Lys 105 Asp Pro G n Phe Asp Arg Hi s G u 170 l e Asp 185 Ser G y GAy y Ala Asp G u Su I e Asp Al a 75 Ar g Ser Tyr Pr o Thr 155 Su I e I e Cys Al a 235 SIle Val Al a Ar g Al a Ar g G u G n Val 140 G u Al a Thr Thr Ser 220 l le Al a Lys Ser Phe G n Sy Val SIle 125 Tr p Ser Hi s Sy G n 205 Ser Phe Al a Thr Val Al a Val G n Asp 110 Hi s Al a Leu Ar g Tyr 190 Phe Pr o Val G u Lys Phe Ser Al a Phe I e G u Met Sy Al a 175 Thr bet Lys Pr o SIe Ser Phe Asn Sy Asp Pr o Al a G u Met 160 Val Sy Pr o G u Hi s 240 Lys Ser Leu Val Thr Al a G y Iet Hi s Pro 250 255 <210> 431 Page 553 seql i st cor r ect ed. t xt <211> 1545 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1522) <223> RXA00955 <400> 431 gatggtgcac aaaaggcgct ttccttgctt gccgacggca aagcacgaag agatcgatta ctcagaaaag gagtcttcca ccacccaggc atggttggcc ct g Leu gag G u aaa Lys gcg Al a at t I e t ac Tyr gat Asp ct g Leu t ac Tyr gaa G u 150 at c SIle ct g Leu t cc Ser aca Thr at t SIle acc Thr ttc Phe gag G u agc Ser gat Asp aaa Lys 120 ggt Gy t ac Tyr acc Thr gcg Al a gat Asp 200 ttg Leu gct Al a tct Ser at g Met t ac Tyr at t SIle ct c Leu ttc Phe gat Asp gca Al a gtt Val 170 at c l Ie gat Asp gaa G u cgc Ar g ct g Leu gag G u cag G n 75 t cc Ser gct Al a at c SIle gcc Al a ct c Leu 155 at t SIle ttt Phe cgt Ar g agc Ser at c SIle ttt Phe tgc Cys ccg Pr o gt g Val acc Thr at t SIle at c l Ie 140 gct Al a gat Asp ggc Gy t ca Ser at c SIle gct Al a gat Asp 45 aag Lys ggt Gy ct g Leu gtt Val gat Asp 125 ct g Leu gcc Al a gag Gu gt c Val cgt Ar g 205 gt c Val cac Hi s t cc Ser t cc Ser gaa G u tgc Cys gcc Al a 110 cct Pr o ct c Leu gag Gu gag Gu aac Asn 190 cgc Ar g gag Gu 15 gt g Val ct c Leu gca Al a at c SIle gag G u 95 gct Al a gt c Val at g Met gct Al a gaa Gu 175 cac Hi s ct g Leu ggt Gy gat Asp aac Asn tcg Ser gct Al a 80 ccg Pr o acc Thr cag G n ct c Leu gcg Al a 160 gt c Val cgc Ar g t cc Ser at g act lMt Thr 1 cgt cgc Arg Arg gtg gat Val Asp cag ggt G n Gy cct tct Pro Ser cgc gtg Arg Val gat cgt Asp Arg tct cat Ser His gt a cac Val Hi s 130 tct gtg Ser Val 145 cgt ttt Arg Phe gcc cgc Al a Ar g aac ct g Asn Leu aag ct c Lys Leu 210 agt Ser gga Gy gcg Al a agg Ar g ttg Leu t ac Tyr ttt Phe ctt Leu 115 gcg Al a ctt Leu gat Asp gcc Al a cat Hi s 195 at t SIle aat aat Asn Asn cac Hi s ctt Leu gga Gy gga Gy tct Ser ggt Gy 100 ccg Pr o gcg Al a gat Asp ct g Leu at c l Ie 180 gat Asp cca Pr o ctg Leu cca Pr o ggg Gy at g let cgc Ar g ggc Gy gtg Val cgt Ar g gat Asp gat Asp 165 aag Lys ctg Leu gca Al a Page 554 gat gcc gtg ctc gtg tct gag tc Asp Ala Val Leu Val Ser u Se 215 220 seql i st cor r ect ed. t xt t ggc gtg cgc gat ac r y Val Arg Asp Th 225 c gaa acc gtc r G u Thr Val cgc Ar g 230 acc Thr ccc Pr o cgc Ar g cca Pr o ccc Pr o 310 gat Asp cag G n gag G u ccc Pr o ct a Leu 390 gct Al a at c SIle ttg Leu gct Al a t cc Ser 470 ct a Leu cag G n aaa Lys gcg Al a 280 aat Asn ct g Leu ctt Leu agc Ser gga Gy 360 ggg Gy ctt Leu gt g Val ccg Pr o at c SIle 440 gcg Al a ggg Sy aac Asn 250 tgc Cys gcg Al a t ca Ser t ac Tyr gac Asp 330 gaa G u cag O n gaa G u gcc Al a gcc Al a 410 aac Asn tct Ser gac Asp cac Hi s 235 gt c Val gga G y gt c Val cgt Ar g gt c Val 315 ggc Sy gca Al a gt c Val gt g Val cat Hi s 395 gct Al a gct Al a ggc G y gcc Al a t cc Ser gat Asp ct c Leu t ac Tyr gaa G u 300 gcg Al a at c SIle gaa G u cag G n gca Al a 380 gaa G u gt g Val gcg Al a gt g Val ggc G v aat Asn ct g Leu acc Thr ggc G y 285 aca Thr gt c Val ttc Phe aag Lys gt c Val 365 gct Al a ggt Sy aag Lys cag G n gaa G u 445 gcg Al a gca Al a gca Al a t ca Ser 270 ggg G y ttg Leu agc Ser gcc Al a gca Al a 350 tgg Tr p gcg Al a ggc Sy gca Al a gca Al a 430 t ac Tyr ct g ttc Phe gcc Al a 255 cca Pr o ct c Leu caa G n cgt Ar g gt a Val 335 ttg Leu cgc Ar g gt g Val agc Ser aag Lys 415 ct c Leu ccc Pr o ct g ct c Leu 240 cgc Ar g agt Ser at c SIle aaa Lys cgc Ar g 320 caa G n at c SIle gcg Al a gag G u ggg Sy 400 tct Ser gct Al a gcc Al a aaa gtt Val gaa G u gca Al a ttc Phe at c SIle 305 acc Thr at c SIle gcc Al a at c l Ie ggt Sy 385 gaa G u ttg Leu gt g Val ggt Gty at t ggc Gy tta Leu gca Al a gaa G u 290 at c SIle t cc Ser cac Hi s gcc Al a tcg Ser 370 gac Asp gt a Val ct c Leu ggc G y gca Al a 450 cag G n t ac Tyr 260 acc Thr gca Al a gca Al a t ac Tyr cca Pr o 340 cgt Ar g t cc Ser gat Asp gac Asp gga G y 420 gca Al a acg Thr ct g Leu 245 ggc G y gct Al a tcg Ser gag G u aag Lys 325 ct g Leu gaa G u agc Ser aag Lys tgg Tr p 405 ggc G y ggt G y tgg Tr p 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1545 tta gcg acc atc Leu Ala Thr Ile 460 Leu Leu Lys Il e 465 aca ttc cat tac taaaggttta aataggatca tga Thr Phe His Tyr Page 555 seqi i st car r ect ed. t xt <210O> 432 <21 1> 474 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 432 l'bt Thr Ser Asn Asn Leu Pro Thr Val Ar g ValI O31n Pr o Ar g As p Ser ValI Ser 145 Ar g A a As n Ly s As p 225 ValI O31u A a Ph e I Ie 305 G31y A a Ar g Le u Ty r Ph e Le u 115 A a Le u As p A a Hi s 1 95 li1e O31u Ser ValI O31n 275 O31u A a Le u Pr o G31y vb t Ar g (31y ValI Ar g As p As p 1 65 Ly s Le u A a ValI Le u 245 G31y A a Ser O31u O31u Ser Ar g Ar g A a Ty r cys Ph e 135 O31u Le u G31y I Ie A a 215 O31n Ser As n A a Ar g 295 As n I Ie Th r 40 Ph e O31u Ser As p Ly s 120 G31y Tyr Th r A a As p 200 ValI Le u O31n Ly s A a 280 As n Le u Ar g Ar g I Ie Hi s O31y His 1 05 As p A a A a O31u Ly s 1 85 Le u Le u O31y O31u ValI 265 O31y ValI Ar g Le u 10 A a Ser vb t Ty r I Ie 90 Le u Ph e As p A a ValI 1 70 li1e As p ValI O31y As n 250 cys A a Ser Ty r O31u Ar g Le u O31u O31n 75 Ser A a I Ie A a Le u 1 55 li1e Ph e Ar g Ser Hi s 235 ValI G31y ValI Ar g ValI 315 Ser I Ie Ph e cys Pr o ValI Th r le le 140 A a As p G31y Ser O31u 220 Ser As p Le u Ty r O31u 300 A a I Ie A a As p Ly s G31y Le u ValI As p 125 Le u A a O31u ValI Ar g 205 Ser As n Le u Th r G31y 285 Th r ValI ValI Hi s Ser Ser O31u cys A a 110 Pr o Le u O31u O31u As n 1 Ar g G31y A a A a Ser 270 G31y Le u Ser O31u ValI Le u A a I Ie O31u A a ValI vb t A a O31u 1 Hi s Le u ValI Ph e A a 255 Pr a Le u O31n Ar g O31y As p As n Ser A a Pr a Th r O31n Le u A a 1 ValI Ar g Ser Ar g Le u 240 Ar g Se r I Ie Ly s Ar g 320 Thr Ser O31y Tyr Lys Asp Leu Leu Val Asp O31y I Ie Phe Ala Val O31n Page 556 sec 325 Ilie His Ala Pro Leu On Asp Ser\ 3403 Al a Al a Val Ar g 31u G31u Val (31y F 355 360 li1e Ser I'b t Ser Ser Pr o Leu G31y A 370 375 G31y As p Val As p Lys Leu I Ie LeuA 385 390 G31u Val Phe As p Tr p Al a Thr Val F 405 Leu Leu Al a 31y G31y I Ie Ser Pr oA 420 4 Val G31y Cys Al a G31y Leu As p I IeA 435 440 G31y Al a G31y Thr Tr p Al a G31y Al a L 450 455 li1e Leu Al a Thr I Ie Ser Thr Phe F- 465 470 <210O> 433 <21 1> 494 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 OIDS <222> (471) <223> RXA02814 <400> 433 gcc aaa aac cta gaa gaa cac tcc t Al a Lys Asn Leu 03 u 03 u Hi s Se r T 1 5 at c ct g gaa cca ct g t gc t ca caa t Ilie Leu 03 u Pr o Leu Cys Ser O31n F ct g acc aaa acc aac gaa at g t gg c Leu Thr Lys Thr Asn 03 u I'b t Tr p F- 40 acc ct C aag t ac cca cac at c acc g Thr Leu Lys Tyr Pr o Hi s Ilie Thr A 55 cac ccc acc ccc gcg at c t gt ggc a Hi s Pr o Thr Pr o Al a Ilie Cys G31y T 70 ct C at c at c gaa gcg gaa t cc ccc c Leu Ilie Ilie O31u Al a O31u Ser Pr o A ggc tgg tgt gac tcc acc gga gac g (3y Tr p Cys Asp Ser Thr (3y Asp C 100 1 lIi st cor r ect ed. t xt 330 (al Ou Ala Ou Lys ~45 ~ro O31n Va 1O31n Val 365 1 a O31u Val Al a Al a 380 osp Al a Hi s 03 u 03 y 395 ~ro Al a Al a Val Lys 410 osp As n Al a Al a 03 n .25 osn Ser G31y Val O31u 445 .ys As p Al a 03 y Al a 460 fi s Ty r Al a 350 Tr p Al a G31y Al a Al a 430 Tyr Le u ct g Le u gt t ValI at c I Ie at a I Ie gcc Al a gga G31y gcc Al a 110 335 Le u Ar g ValI Ser 415 Le u Pr o Le u cgc Ar g cct Pr o gt t ValI cga Ar g g aa O31u gca Al a at c I Ie ac yr t c ~he 25 ac fi s ca ~I a c c hir ga ~r g gc 05 gt g gt c aac Val Val As n 10 gac gcc cca Asp Al a Pr o ct C gca aca Leu Al a Thr ct a gaa ct a Leu 03 u Leu ccc acc gac Pr o Thr Asp 75 aac t tc t ac Asn Phe Tyr 90 gaa tac at g (3 u Tyr N/t Page 557 C ac Hi s ac a Thr ccc Pr o gcc Al a gcc Al a gcc Al a gt a ValI ac c Thr g aa O31u G31y ac a Thr gcc Al a gcc Al a cgc Ar g seqI i st car r ect ed. t xt t gc gcc gaa gt a t cc gaa gac gga acc t gg gcc aga gca t gg gca ggc 384 Cys Al a (31u Val Ser (31u Asp G31y Thr Tr p Al a Ar g Al a Tr p Al a (31y 115 120 125 gga ggc atc gtc gcc gaa tca gac gcc caa gaa gag ttt gat gaa acc 432 G31y (1y lle Val Al a 1u Ser Asp Al a (1n (1u (1u Phe As p 1u Th r 130 135 140 acc gcg aag ct ccaa acc at cat gcgc tcg ctt ggt tt gt gagat gt gg 481 Thr Al a Lys Leu O31n Thr Ilie Nib t Ar g Ser Leu O31y Leu 145 150 155 tcttaaaaca ccg 494 <210> 434 <211> 157 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 434 Al a Lys Asn Leu O31u O31u Hi s Ser Tyr Val Val Asn Hi s Leu Ar g Thr 1 5 10 Ilie Leu Gu Pro Leu Cys Ser G n Phe Asp Al a Pro Th r Val Pro G u 25 Leu Thr Lys Thr Asn 31u I'b t Tr p Hi s Leu Al a Th r Pr o Ilie Va1(3 Gy 40 Thr Leu Lys Tyr Pr o Hi s Ilie Thr Al a Leu O31u Leu Al a Ilie Ar g Thr 55 Hi s Pr oThr Pr o Al a Ilie Cys G31y Thr Pr oThr Asp AiaAia (1u Al a 70 75 Leu Ilie Ilie 31u Al a O31u Ser Pr o Ar g Asn Phe Tyr Al a G31y Ala Ala 90 G31y Tr p Cys Asp Ser Thr G31y Asp G31y O31u Tyr I'b t Val Al a I Ie Ar g 100 105 110 Cys Al a (31u Val Ser (31u Asp G31y Thr Tr p Al a Ar g Al a Tr p Al a G31y 115 120 125 G31y (1y Ile Val Al a 1u Ser Asp Aa (1n (1u 1u Phe Asp (1u Th r 130 135 140 Thr Al a Lys Leu O31n Thr Ilie Nib t Ar g Ser Leu G31y Leu 145 150 155 <210> 435 <211> 803 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221> CIDS <222> (780) <223> RXA00229 <400> 435 gag gcg aaa ggc ctc gcg cag gga cgt gcg acg gtg tac agg cgc atc 48 Ou Ala Lys Gy Leu Ala On Gy Arg Ala Thr Val Tyr Arg Arg Ilie 1 5 10 Page 558 seql i st cor r ect ed. t xt gac acg ctt ggg tcg cgt gct tcc ggg caa gat tta aat acg ctt ctc 96 Asp Thr Leu y Ser Arg Ala Ser Gy G n Asp Leu Asn Thr Leu Leu 25 gac gcc gcc ctc tac ctt ggc ttc agc ggc ctg aac atc act cac ccg 144 Asp Al a Al a Leu Tyr Leu G y Phe Ser G y Leu Asn I e Thr His Pro 40 tac aag caa gca gta tta ccc ctg ctt ggc gaa gtc tcc gaa caa gcc 192 Tyr Lys G n Ala Val Leu Pro Leu Leu y G u Val Ser G u G n Ala 55 acc caa ctc ggc gca gtg aat act gtc gtt atg gac gcc acc ggc cac 240 Thr G n Leu G y Ala Val Asn Thr Val Val Met Asp Ala Thr G y His 70 75 acc acc ggc cac aac acc gac gtc tcc gga ttt ggc cgc gga atg gaa 288 Thr Thr G y His Asn Thr Asp Val Ser G y Phe G y Arg G y Iet G u 90 gaa ggc ctc ccc aac gcc aag ctc gat tcc gtc gtg cag gtc ggc gcc 336 G u y Leu Pro Asn Al a Lys Leu Asp Ser Val Val G n Val y Ala 100 105 110 ggc ggc gta gaa aac gca gtg gca tac gcc ctg gtc acc cac ggt gtg 384 G y G y Val G u Asn Al a Val Al a Tyr Al a Leu Val Thr His G y Val 115 120 125 cag aaa ctt cag gtc gct gac ctc gac act tcc cgc gcg cag gca ctg 432 G n Lys Leu G n Val Al a Asp Leu Asp Thr Ser Arg Ala G n Ala Leu 130 135 140 gca gat gtc atc aac aac gca gtc ggc cgt gaa gcc gtc gtg gga gta 480 Ala Asp Val lie Asn Asn Ala Val Gy Arg u Ala Val Val Gy Val 145 150 155 160 gac gcc cgc ggc atc gaa gac gtc atc gcc gcc gcc gac gga gta gtc 528 Asp Ala Arg Gy lle u Asp Val Ile Ala Ala Ala Asp y Val Val 165 170 175 aac gca acc ccc atg gga atg cca gca cac ccc ggc acc gcc ttt gat 576 Asn Ala Thr Pro IVet G y Iet Pro Ala His Pro G y Thr Ala Phe Asp 180 185 190 gtc agc tgc ctc acc aag gat cac tgg gtt ggc gac gtc gtg tac atg 624 Val Ser Cys Leu Thr Lys Asp His Trp Val G y Asp Val Val Tyr IVet 195 200 205 ccc atc gaa act gaa ctt ctc aaa gcc gcc cgt gcc ctc ggc tgc gaa 672 Pro lie G u Thr G u Leu Leu Lys Ala Ala Arg Ala Leu y Cys G u 210 215 220 acc ctc gac gga acc cgc atg gca atc cac caa gcc gtc gat gcc ttc 720 Thr Leu Asp G y Thr Arg IVet Ala IIe His G n Ala Val Asp Ala Phe 225 230 235 240 cga ctg ttc acc ggc ctc gaa ccc gac gtc tcc cgc atg cgg gaa act 768 Arg Leu Phe Thr y Leu G u Pro Asp Val Ser Arg IVet Arg G u Thr 245 250 255 ttc ctg tcc ctc taaaagagtc agtaaaacct cga 803 Phe Leu Ser Leu 260 <210> 436 <211> 260 <212> PRT Page 559 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <400> 436 G u Al a Lys G y Leu Ala n G y Ar g 1 Asp Asp Tyr Thr Thr G u Gy G n Al a 145 Asp Asn Val Pr o Thr 225 Ar g Phe Leu Al a G n Leu Gy Leu Val 115 Leu Val Ar g Thr Cys 195 G u Asp Phe Ser 5 Ser Tyr Val Al a Asn Asn Asn Val Asn SIle 165 bet Thr G u Thr Gy 245 Ar g Leu Leu Val 70 Thr Al a Al a Al a Asn 150 G u Gy Lys Leu Ar g 230 Leu Al a Gy Pr o Asn Asp Lys Val Asp 135 Al a Asp lbet Asp Leu 215 t u Gu Ser Phe 40 Leu Thr Val Leu Al a 120 Leu Val Val Pr o Hi s 200 Lys Al a Pr o Gy Ser Leu Val Ser Asp 105 Tyr Asp Gy I e Al a 185 Tr p Al a l Ie Asp Al a 10 G n Gy Gy Val Gy 90 Ser Al a Thr Ar g Al a 170 Hi s Val Al a Hi s Val 250 Thr Asp Leu G u let Phe Val Leu Ser G u 155 Al a Pr o Gy Ar g G n 235 Ser Val Leu Asn Val Asp Gy Val Val Ar g 140 Al a Al a Gy Asp Al a 220 Al a Ar g Tyr Asn SIle Ser Al a Ar g G n Thr 125 Al a Val Asp Thr Val 205 Leu Val let Ar g Thr Thr G u Thr Sy Val 110 Hi s G n Val Sy Al a 190 Val Sy Asp Ar g Ar g Leu Hi s G n Sy bet Sy Sy Al a Sy Val 175 Phe Tyr Cys Al a G u 255 <210> 437 <211> 927 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(904) <223> RXA02093 <400> 437 ggcaggaatt tcccgaaaac ttccaccaat aatcaagcca tatcccacac aatcaggcat Page 560 seql i st cor r ect ed. t xt ttgctcttcc tttgctccgc atgagtataa aatcactgt c atg gtc aac tac gtc let Val Asn Tyr Val gac Asp cat Hi s t ac Tyr ggt Gy aag Lys cgc Ar g gga Gy cgc Ar g gcc Al a gt c Val 150 ggt Gy aat Asn ttt Phe gca Al a aag Lys 230 cag G n gaa G u gtt Val t ac Tyr cgt Ar g gat Asp cgt Ar g aac Asn aac Asn 120 gct Al a gcc Al a gaa G u acc Thr gag G u 200 ccc Pr o acc Thr cac Hi s acc Thr ttc Phe gct Al a ct g Leu at c SIle gtt Val gac Asp aat Asn gtt Val aac Asn t cc Ser 170 at g Met gaa G u gag G u gac Asp t ac Tyr 250 ct g Leu cac Hi s gtt Val aac Asn cca Pr o 75 aac Asn tac Tyr gca Al a gca Al a cac Hi s 155 gca Al a gga Gy gt a Val acc Thr ggc Gy 235 acc Thr tgc Cys aac Asn gcc Al a at t SIle ct c Leu acc Thr act Thr cga Ar g gct Al a 140 acc Thr act Thr at g Met gac Asp cca Pr o 220 gga gay gga Gy at c SIle tgg Tr p cca Pr o 45 cgc Ar g at c SIle at c SIle gcg Al a gt a Val 125 ctt Leu acc Thr gtt Val aat Asn cga Ar g 205 ct g Leu gaa G u gtt Val tct Ser ctt Leu gca Al a ggc Gy gat Asp gt c Val gt g Val 110 gct Al a gct Al a ggt Gy ccg Pr o gga Gy 190 gcc Al a at t SIle gtt Val ctt Leu ct c Leu 15 t ac Tyr gat Asp gca Al a gag G u aac Asn 95 t ac Tyr at c l Ie gag G u tct Ser gaa G u 175 cct Pr o gac Asp aag Lys gcc Al a cca Pr o 255 gct Al a gct Al a at c SIle ggt Gy ttg Leu 80 aat Asn cac Hi s aag Lys t at Tyr gcg Al a 160 gac Asp gac Asp gt a Val ttg Leu gct Al a 240 acc Thr gct Al a gaa G u acc Thr gt c Val cat Hi s gac Asp ct c Leu gga Gy ggt Gy 145 ct a Leu gca Al a caa G n at c SIle gcc Al a 225 ctt Leu aac Asn cgt Ar g ctt Leu gct Al a t cc Ser cct Pr o gga Gy ctt Leu t cc Ser 130 ct g Leu gct Al a aaa Lys gac Asp ttt Phe 210 aag Lys cag G n gac Asp cca Pr o gga Gy gca Al a at g let t cc Ser cac Hi s gaa G u 115 ggc Gy agt Ser t cc Ser at t SIle gtt Val 195 gac Asp gaa G u gca Al a cag G n t cc Ser ttg Leu gt c Val cca Pr o gca Al a ctt Leu 100 gaa G u ggc Sy ggc Sy cgt Ar g ttg Leu 180 gt a Val tgc Cys aag Lys gca Al a at c SIle 260 aac Asn aac Asn gca Al a t ac Tyr gag G u gtc Val cac Hi s at g let acc Thr t ac Tyr 165 gtt Val tct Ser gta Val ggt G y gag G u 245 att lle Page 561 seqI i st car r ect ed. t xt gct gcg gag gag ttc tcc aag taaatttctc tcccctattt tta Al aAl a a u Gu Phe Ser Lys 265 <210O> 438 <21 1> 268 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 438 I'bt Val Asn Tyr Val Asp Arg O31u Th r A a O31u Thr ValI Hi s As p Le u G31y G31y 145 Le u A a O31n I Ie A a 225 Le u As n Pr a G31y A a vb t Ser Hi s (31u 115 G31y Ser Ser I Ie ValI 1 95 As p O31u A a O31n As n As n A a Tyr O31u ValI Hi s vb t Thr Tyr 1 65 ValI Ser ValI G31y O31u 245 Hi s Tyr G31y Ly s 70 Ar g G31y Ar g A a ValI 150 G31y As n Ph e A a Ly s 230 O31n G31y Le u I Ie Ser I Ie Tyr ValI As n 135 ValI Tr p ValI G31y Ph e 215 O31n Ph e ValI Tyr 40 Ar g As p Ar g As n As n 120 A a A a O31u Thr O31u 200 Pr a Thr Hi s Ar g Ly s O31y ValI Ser Thr 1 05 Pr a ValI Ar g Ty r Pr a 1 85 As p ValI I Ie Le u O31u 265 Thr 10 Ph e A a Le u I Ie ValI 90 As p As n ValI As n Ser 1 70 vb t O31u O31u As p Tyr 250 Le u Hi s ValI As n Pr a 75 As n Tyr A a A a Hi s 1 55 A a O31y ValI Thr O31y 235 Thr cys As n A a I Ie Le u Thr Thr Ar g A a 140 Th r Th r vb t As p Pr a 220 O31y (31y I Ie Tr p Pr a Ar g I Ie I Ie A a ValI 125 Le u Thr ValI As n Ar g 205 Le u O31u ValI Ser Le u A a O31y As p ValI ValI 110 A a A a O31y Pr a O31y 1 A a I Ie ValI Le u Le u Tyr As p A a O31u As n Tyr I Ie O31u Ser O31u 1 Pr a As p Ly s A a Pr a 255 A a A a I Ie O31y Le u As n Hi s Ly s Tyr A a 1 As p As p ValI Le u A a 240 Thr Ile Al aAl a 0 u Phe Ser Lys <210O> 439 <21 1> 951 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 562 seqi i st car r ect ed. t xt <220> <221 CIDS <222> (928) <223> RXA02791 <400> 439 accgtgttca acgacacctt cgaagagcac gaagccgaca ggcgttctag acagcaaccg ataaggatca gcgaataaaa t tgagcaagc t t tgaacagt act Thr cca Pr o g aa (31u t cc Ser aaa Ly s gcc A a gcc A a ct c Le u ggc G31y gcc A a 1 50 ac g Thr cat Hi s gca A a gt c ValI cgg Ar g ct c Le u g ac As p gct A a gca A a ggc G31y aac As n ggc G31y 120 ac c Thr at c I Ie tt c Ph e c ac Hi s gca A a 200 t ac Tyr gcc A a aac As n tt t Ph e g aa G u ct t Le u gca A a g ac As p t ca Ser cgc Ar g gt g ValI g aa (3u 1 70 g aa (3u ct c Le u ccc Pr o gt a ValI acc Thr gag (31u acc Thr gaa (31u 75 aac As n gt c ValI ct g Le u ccc Pr o ct c Le u 155 acc Thr gcc A a gaa (31u t gg Tr p ct c Le u ggc G31y t gc cys t ac Tyr tt c Ph e ac c Thr g ac As p gcc A a gcc A a 140 aac As n ccc Pr o g ac As p g ac As p cca Pr a 220 ggc G31y t at Tyr ac c Thr 45 t gc cys gcc A a tt g Le u ggc G31y G31y 125 at c I Ie cgc Ar g ac c Thr gt c ValI ac c Thr 205 ac a Thr t ca Ser aaa Ly s ggc G31y gga (31y g ac As p ct g Le u at c I Ie 110 aaa Ly s t gg Tr p t cc Ser ac c Thr gt a ValI 1 90 ct t Le u cca Pr a ccc Pr a 15 gcc A a g ac As p tt c Ph e g aa (31u cgc Ar g 95 ag g Ar g c ac Hi s gca A a g at As p tt g Le u 1 75 gt c ValI gcg A a ct c Le u at c I Ie ct c Le u at g vb t t cc Ser gt a ValI 80 ac g Thr gga G31y gcc A a ct c Le u cgc Ar g 1 60 gcc A a t ct Ser at c I Ie gt a ValI tt g Le u 1 gag (31u gga G31y ct c Le u gt c ValI ac c Thr gcc A a gct A a at c I Ie at c I Ie 145 ac c Thr t ac Tyr ac a Thr gcc A a g aa (31u 225 cat Hi s ct g Le u ccc Pr a ac c Thr g aa (31u ac c Thr ct c Le u gt c ValI 130 g aa (31u gcc A a gcc A a gt g ValI cca Pr a 210 gt c ValI t cc Ser g ac As p ggc G31y at g vb t cgc Ar g gga G31y ggt G31y 115 at c I Ie gcc A a g aa (31u ccg Pr a ccc Pr a 1 gt c ValI ac a Thr aaa Ly s c aa O31n at c I Ie ccg Pr a gcc A a t gg Tr p 1 00 g aa G31u G31y G31y ct g Le u ct c Le u 1 t cc Ser ct c Le u cga Ar g ggt t ct cac G31y Se r Hi s at c le t cc Ser t gg Tr p gt c ValI t ct Ser t gc cys cgc Ar g ct c Le u t cc Ser gt c ValI c aa O31n 1 gag O31u gca A a g ac As p gcc A a Page 563 aaa ggc ct C aaa gct gt C gga Lys G31y Leu Lys Ala Val G31y 230 235 tac gga cag t tt gaa caa tt c Tyr 03 y 03 n Phe 03 u 03 n Phe 250 at g cgt gag gct t tg gaa gag I'b t Ar g O31u Ala Leu O31u O31u 265 t aagt ccccg ccacct cct c aac <210> 440 <211> 276 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ar seqI i st car r ect ed. t xt ggc cac gt c at g ct g gca cac cag G31y Hi s Val I'b t Leu Ala Hi s O31n 240 acc gga at g gat gca ccc cgc gat Thr G31y N/t Asp Ala Pro Ar g Asp 255 260 tct tta ggc atc tca gaa gaa cac Ser Leu 31y Ilie Ser 31u G31u Hi s 270 275 <400> 440 Leu 03 y Ser Hi s I I e Th r G31u G31y Le u ValI Thr A a A a I Ie I Ie 145 Thr Tyr Thr A a G31u 225 Le u Ser As p G31y vb t Ar g G31y G31y 115 li1e A a G31u Pr o Pr o 1 95 ValI Thr Hi s Ser Tr p ValI Ser cys Ar g Le u Ser ValI O31n 1 65 O31u A a As p A a Ser 245 Pr o O31u Ser Lys5 70 A a A a Le u G31y A a 150 Thr Hi s A a ValI Lys5 230 Tyr Hi s ValI Tyr G31y Ph e I Ie As p G31y G31y 135 Ar g Le u Le u I Ie I Ie 215 G31y (31y Ar g Le u As p 40 A a A a G31y As n G31y 120 Thr I Ie Ph e Hi s A a 200 Tyr Le u (31n A a Hi s Ar g As p A a Ser Thr 1 A a A a Thr As p I Ie 1 G31y As p Lys5 Ph e A a 10 As n Ph e O31u Le u A a 90 As p Ser Ar g ValI O31u 1 70 O31u Le u Pr o A a ValI Thr O31u Thr O31u As n ValI Le u Pr a Le u 1 Thr A a O31u Tr p ValI 235 Le u G31y cys Tyr Ph e Thr As p A a A a 140 As n Pr a As p As p Pr a 220 G31y G31y Tyr Thr cys A a Le u G31y G31y 125 li1e Ar g Thr ValI Thr 205 Thr G31y Pr a A a As p Ph e O31u Ar g Ar g Hi s A a As p Le u 1 ValI A a Le u ValI (3 u (3 n Phe Thr 03 y N/t Asp 250 255 Page 564 seqI i st car r ect ed. t xt Ala ProArg AspAla I'bt Arg Ou Ala Leu Ou Ou Ser Leu Gy Ilie 260 265 270 Ser G u Gu Hi s 275 <210O> 441 <21 1> 693 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> 101). (670) <223> RXA01699 <400> 441 ct gcagaaat t cggcggt ga ct ccct gagc gaaaccaaga aaaaacat tg aggaacgaat gaaat tcgaa ggt t tagagg gcaacat t ga cacct acct c at g gag cgt aat gaa 11! [Vb t O31u Ar g As n O31u gt g ValI t gc CYS t cc Ser g ac As p gt g ValI c ac Hi s gga G31y gt g ValI aac As n t ac Tyr 1 50 ac c Th r g at As p t gc cys at t lIe g ac As p ag c Ser gcc Al a gt g ValI t gg Tr p 120 cgt Ar g aac As n cga Ar g at t lIe at c I Ie cgt Ar g ct g Le u ct c Le u gca Al a ac a Th r g ac As p cgc Ar g gt g ValI cgc Ar g 1 70 cac Hi s gt g ValI cga Ar g at t I e ggc G31y 75 ct g Le u gaa (31u gt g ValI ccc Pr a aaa Ly s 155 acc Th r tt a Le u gt t ValI tt a Le u gag (31u gag (31u aaa Ly s t cc Ser cca Pr a gt g ValI 140 gt g ValI aac As n g at As p ct t Le u gcg Al a 45 cgc Ar g cca Pr a t cc Ser ac c Th r gt a ValI 125 ct g Le u cgc Ar g aac As n cat Hi s gt g ValI cgc Ar g gcc Al a gcc Al a t cc Ser cgt Ar g 110 g aa (31u c aa O31n ac c Th r cgc Ar g c aa O31n 15 ggt G31y gcc Al a ac c Th r tt c Ph e gga G31y 95 g aa O31u g aa O31u gcc Al a ccg Pr a ag c Ser 1 75 t ca Ser tt g Le u tt a Le u gga G31y cgc Ar g 80 gt g ValI ct g Le u ggc G31y gcc Al a tt g Le u 1 60 ccc Pr a g ac As p gga G31y act Th r 50 gcc Al a ct c Le u ag c Ser aaa Ly s ag g Ar g 130 ccc Pr a g aa O31u c aa (31n ac c Th r gct Al a 35 g aa O31u t gt cys gag O31u tt g Le u ggc G31y 115 cgc Ar g gcc Al a gag O31u gt g ValI t ct Ser 20 gga G31y ct c Le u ggc G31y gcc Al a gga G31y 1 00 c ag O31n ac c Th r gag (31u gt g ValI gt g ValI 1 g aa (31u aaa Ly s gt c ValI gcc Al a at c I Ie ggc G31y g ac As p gca Al a c ac Hi s gca Al a 1 gca Al a Page 565 seql i st cor r ect ed. t xt gca gtg ttg cat cat cta gaa atc gat taat t aaacc gggcacctga Ala Val Leu His His Leu u Ile Asp 185 190 tta <210> 442 <211> 190 <212> PRT <213> Corynebact eri um gl ut am cum <400 Mbt 1 Asp Pr o Asn Lys G u Val Leu SIle Asp 145 Tyr G n 442 Su Arg Asn Asp Thr Ser G y Al a G y Thr G u Leu Al a Cys G y Leu G u Al a Ser Leu G y 100 Lys G y G n 115 Arg Arg Thr 130 Pr o Al a G u G u G u Val G n Val Val 180 G u 5 G u Lys Val Al a l Ie Gy Asp Al a Hi s Al a 165 Val Asn Asp G n Ser Thr Ser Phe Val Ser Val G u 135 Ar g Tyr Pr o 25 Gy G u G u G u Leu 105 I le Ser Leu Leu Hi s 185 Hi s Val Ar g SIle Gy Leu G u Val Pr o Lys 155 Thr Asp Leu Al a Ar g Pr o Ser Thr Val 125 Leu Ar g Asn Hi s Val Ar g Al a Al a Ser Ar g 110 G u G n Thr Ar g G n Gy Al a Thr Phe Gy G u G u Al a Pr o Ser 175 Al a Al a Val Leu His Leu u Ile <210> 443 <211> 959 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (97)..(936) <223> RXA00952 <400> 443 catcctcgtc tccctatccg gccgtggcga caaggacgtt gaccacgtgc gccgcaccct cgaagaaaat ccagaactga tcctgaagga caaccg atg agc cgt tac gac gat 114 IVet Ser Arg Tyr Asp Asp 1 Page 566 ctt ttt gca cgc Leu Phe Ala Arg ctc gac acg gca Leu Asp Thr Ala eql i st cor r ect ed. t ggg gag ggc gcc Gy Gu y Al a ttt gtt ccc ttc Phe Val Pro Phe at c SIle aca Thr t cc Ser gca Al a cgc Ar g ggc Gy gct Al a gaa G u 135 at t SIle t cc Ser acc Thr gac Asp at c SIle 215 ggt Gy gaa G u aag Lys ct g Leu at c SIle cca Pr o gac Asp cgc Ar g gtt Val 105 gct Al a gca Al a at c SIle gca Al a acc Thr 185 at c SIle t cc Ser at c SIle gag G u gat Asp 265 gac Asp gct Al a gcc Al a ggc Gy gcc Al a ttc Phe gca Al a ttt Phe ccg Pr o 155 aag Lys cgt Ar g aaa Lys cag G n ggt Gy 235 ccg Pr o act Thr cct Pr o ggc Gy gat Asp 60 gcc Al a tac Ty r acc Thr gac Asp tct Ser 140 gcc Al a ggc Gy gaa G u ttt Phe cac Hi s 220 t cc Ser aac Asn gag Gu t ca Ser gca Al a ggc Gy acc Thr cca Pr o cgt Ar g t cc Ser 125 gca Al a aac Asn t ac Tyr t ca Ser gat Asp 205 gt g Val gcg Al a ccg Pr o ttc Phe cca Pr o 30 gat Asp ccc Pr o gt a Val gag Gu ggc Gy 110 at c SIle gca Al a gcc Al a at c SIle t cc Ser 190 ggc Gy gca Al a at c l Ie t cc Ser at c SIle 270 gag G u gca Al a acc Thr gac Asp gtt Val ttg Leu ct c Leu gct Al a agc Ser t ac Tyr 175 acc Thr gca Al a gac Asp acc Thr acc Thr 255 tct Ser gag G u ct g Leu gt c Val agc Ser 80 ccc Pr o gat Asp ct g Leu gca Al a gag G u 160 gcc Al a gac Asp ccc Pr o gcg Al a aag Lys 240 at t SIle gcg Al a gct Al a gaa Gu gcg Al a gca Al a at c SIle cgc Ar g cca Pr o gca Al a 145 aaa Lys at c l Ie ggc Gy at c SIle at t l Ie 225 at c SIle cga Ar g at g lbt ttc Phe ctt Leu gaa Gu ct c Leu gga Gy ttc Phe gac Asp 130 gcc Al a acc Thr t cc Ser ct g Leu ct c Leu 210 gca Al a at t SIle gat Asp cag G n ggc Gy t cc Ser gag Gu at g lbt t ac Tyr 115 gt c Val gga Gy ct c Leu cgc Ar g t cc Ser 195 ttg Leu gcg Al a gct Al a at g lbt at c SIle gt a Val cac Hi s cag G n ct c Leu 100 caa G n cca Pr o at t SIle gag G u gac Asp 180 gca Al a ggc Gy ggt Gy t cc Ser gac Asp 260 at c SIe cct Pr o ct c Leu at c SIe at c SIe gag Gu gtc Val gat Asp ggt Gy 165 ggc Gy gtg Val ttc Phe gct Al a cac Hi s 245 ggt Gy aag gca gcg acc aag Lys Al a Al a Thr Lys 275 Page 567 seql i st cor r ect ed. t xt aag gtt taggccttta aatgtggcaa tgt Lys Val 280 <210> 444 <211> 280 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 444 IVet Ser Arg Tyr 1 G y Al a Phe Val Ala Phe G n Ile G u Leu G y Val Ala u Ser His Al a Leu G u G n Ile G y Iet Leu 100 Arg Phe Tyr G n 115 Pro Asp Val Pro 130 Al a Al a G y I e 145 Lys Thr Leu G u Ile Ser Arg Asp 180 G y Leu Ser Al a 195 l e Leu Leu G y 210 i e Al a Al a G y 225 lie Ile Ala Ser Arg Asp IVet Asp 260 IVet Lys Ala Ala 275 <210> 445 <211> 1237 Asp Asp 5 Pro Phe Ile Ser Pro Phe Leu Arg 70 Ile Lys lie Tyr G u Phe Val Arg Asp Pro 150 G y Val 165 G y Val Val Val Phe G y Al a Ser 230 His Cys 245 G y Leu Thr Lys Leu SIle Thr Ser Al a Ar g Sy Al a G u 135 I le Ser Thr Asp SIle 215 Sy G u Lys Lys Phe bet Al a 40 Asp Leu Val Asn G u 120 Sy Tyr Al a Sy Asn 200 Ser Al a Sy Lys Val 280 Al a Leu 25 l le Pr o Asp Ar g Val 105 Al a Al a SIle Al a Thr 185 I le Ser l Ie G u Asp 265 Leu Asp Al a Al a Sy 75 Al a Phe Al a Phe Pr o 155 Lys Ar g Lys G n Sy 235 Pr o Thr Thr Ser Al a Sy Thr Pr o Ar g Ser 125 Al a Asn Tyr Ser Asp 205 Val Al a Pr o Phe Al a Pr o Asp Pr o Val G u Sy 110 SIle Al a Al a SIle Ser 190 Sy Al a l Ie Ser SIle 270 Sy G u Al a Thr Asp Val Leu Leu Al a Ser Tyr 175 Thr Al a Asp Thr Thr 255 Ser G u G u Leu Val Ser Pr o Asp Leu Al a G u 160 Al a Asp Pr o Al a Lys 240 I e Al a Page 568 seqi i st car r ect ed. t xt <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1237) <223> RXN00956 <400> 445 gccggtgcag gcacgtgggc tggggcgaaa gacgccggcg cgctgctgaa aattttagcg accat ct cca cat tccat ta ct aaaggt tt aaat aggat c aac As n ggc G31y aag Lys ggc G31y t gc CYS tt c Ph e c ag O31n at c I Ie gca A a g ac As p 1 50 gcg A a gt g ValI t ac Ty r ggc G31y tt c Ph e tt c Ph e t ac Ty r aac As n aag Ly s at c I Ie gca A a 120 gcg A a gcc A a gt c ValI g aa O31u ct c Le u 200 t cc Ser gcg A a g ac As p cgc Ar g cca Pr o g aa O31u c ag O31n ac c Th r at g vb t c ag O31n ccc Pr o 1 70 ct g Le u ac c Th r ac g Th r gaa (31u gcg A a g at As p ct c Le u 75 gac As p gt g ValI ggc G31y G31y cag O31n 155 gt g ValI cgc Ar g ccc Pr o ct g Le u t cc Ser ac c Th r t ac Ty r gca A a ct c Le u ct g Le u gca A a ct c Le u 140 ccc Pr o g aa O31u g at As p gcc A a cct Pr o ct g Le u ag c Ser ggc O31y g aa O31u c ac Hi s gcc A a 110 c ag O31n t gc cys gt c ValI ggt O31y ac c Th r 1 90 ccg Pr a gca A a 15 cct Pr a cca Pr a cgc Ar g ggc O31y ggc O31y 95 aag Ly s c ac Hi s gt t ValI t ac Ty r t cc Ser 1 75 gca A a c ac Hi s t ac Ty r gct A a gag O31u cca Pr a aaa Ly s ggt O31y cgc Ar g ggc O31y gt c ValI cgc Ar g 1 ggc O31y ac c Th r cca Pr a at g act l'b t Thr 1 t tc ggt Phe 03 y ct c gac Leu Asp ttc cgc Phe Ar g acc ccg Thr Pr o ggc ttt O3 y Phe gca cac A a Hi s at g ggc Vb t (1y acc gcc Thr A a 130 t ac at g Ty r lb t 145 at g cag Vb t (1n acc ct g Thr Leu ttc cac Phe Hi s ttc cca Phe Pr o 210 gaa aaa gaa 03 u Lys 03 u g aa O31u c ag O31n g aa O31u ct g Le u gcg A a aaa Ly s aaa Ly s 115 ac c Thr ggc O31y ct g Le u aag Ly s gag O31u 1 ac c Thr tt c Ph e ct g Le u g aa O31u ac c Thr cgg Ar g act Thr 1 00 ac c Thr gct A a gcc A a c ac Hi s g ac As p 1 t cc Se r at c I Ie cgt gaa t tc cac aag gt g at c t ct gag gaa gcc aag gca cag at g ct a Page 569 seql i st cor r ect ed. t xt Arg G u Phe His Lys Val Ile Ser G u G u Ala Lys Ala G n Met Leu 215 220 225 gag cgc acc ggc aag ctt ccc gac gtt gtg gtc gcc tgt gtc ggt ggt 835 G u Arg Thr y Lys Leu Pro Asp Val Val Val Ala Cys Val Gy y 230 235 240 245 ggc tcc aac gcc atc ggc atg ttc gca gac ttc att gac gat gaa ggt 883 Sy Ser Asn Ala lle y IVet Phe Ala Asp Phe Ile Asp Asp G u y 250 255 260 gta gag ctc gtc ggc gct gag cca gcc ggt gaa ggc ctc gac tcc ggc 931 Val u Leu Val y Ala u Pro Ala Gy u y Leu Asp Ser y 265 270 275 aag cac ggc gca acc atc acc aac ggt cag atc ggc atc ctg cac ggc 979 Lys His G y Ala Thr IIe Thr Asn G y n lie G y lie Leu His G y 280 285 290 acc cgt tcc tac ctg atg cgc aac tcc gac ggc caa gtg gaa gag tcc 1027 Thr Arg Ser Tyr Leu Iet Arg Asn Ser Asp y G n Val G u G uSer 295 300 305 tac tcc atc tcc gcc gga ctt gat tac cca ggc gtc ggc cca cag cac 1075 Tyr Ser IIe Ser Ala G y Leu Asp Tyr Pro G y Val G y Pro G n His 310 315 320 325 gca cac ctg cac gcc acc ggc cgc gcc acc tac gtt ggt atc acc gac 1123 Ala His Leu His Ala Thr y Arg Ala Thr Tyr Val y Ile Thr Asp 330 335 340 gcc gaa gcc ctc caa gca ttc cag tac ctc gcc cgc tac gaa ggc atc 1171 Ala G u Ala Leu G n Al a Phe G n Tyr Leu Ala Arg Tyr G u y lie 345 350 355 atc ccc gca ctg gaa tcc tca cac gcg ttc gcc tac gca ctc aag cgc 1219 lie Pro Ala Leu u Ser Ser His Ala Phe Ala Tyr Ala Leu Lys Arg 360 365 370 gcc aag acc gcc gaa gta 1237 Ala Lys Thr Ala G u Val 375 <210> 446 <211> 379 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 446 IVet Thr G u Lys G u Asn Leu G y G y Ser Thr Leu Leu Pro Al a Tyr 1 5 10 Phe y G u Phe y G y G n Phe Val Ala u Ser Leu Leu Pro Ala 25 Leu Asp G n Leu G u Lys Ala Phe Val Asp Ala Thr Asn Ser Pro G u 40 Phe Arg G u G u Leu G y y Tyr Leu Arg Asp Tyr Leu y Arg Pro 55 Thr Pro Leu Thr G u Cys Ser Asn Leu Pro Leu Ala Gy G u Gy Lys 70 75 G y Phe Ala Arg lie Phe Leu Lys Arg G u Asp Leu Val His G y G y 90 Page 570 seqli st car r ect ed. t xt 03 n Val Leu Leu Ala Hi s Lys Thr Asn O31n Val I I 1 00 e vb t Thr Tyr 145 vb t Thr Ph e Ph e Ly s 225 A a I Ie O31y O31y O31n 305 ValI ValI Ar g Tyr Ly s 115 Thr O31y Le u Ly s O31u 1 95 Thr O31n ValI As p As p 275 Le u (31u Pr o I Ie O31u 355 Le u Ar g Le u Lys5 O31y 1 65 A a Hi s ValI Le u O31y 245 O31y O31y O31y Ser Hi s 325 As p I Ie Ar g I Ie A a As p 150 A a ValI Tyr Ar g (31u 230 O31y ValI Lys5 Thr Tyr 310 A a A a I Ie A a cys 135 ValI Lys5 As n Le u (31u 215 Ar g Ser (31u Hi s Ar g 295 Ser Hi s (31u Pr o Lys5 375 A a 120 A a A a ValI (31u Le u 200 Ph e Thr As n Le u O31y 280 Ser I Ie Le u A a A a 360 Thr (31y 1 05 (31u Le u Ar g I Ie A a 1 85 O31y Hi s O31y A a ValI 265 A a Tyr Ser Hi s Le u 345 Le u A a Ala Lys Ar g 110 Thr vb t O31n Pr o 1 70 Le u Thr Lys5 Lys5 I Ie 250 O31y Thr Le u A a A a 330 O31n O31u aOu O31y O31y O31n 1 ValI Ar g Pr o ValI Le u 235 O31y A a I Ie vb t O31y 315 Thr A a Ser ValI O31y 125 O31u As n Ser Tr p O31y 205 Ser As p Ph e Pr a As n 285 As n As p Ar g O31n Hi s 365 O31n cys ValI O31y Thr 1 Pr a O31u ValI A a A a 270 O31y Ser Tyr A a Tyr 350 A a Hi s ValI Tyr Ser 1 A a Hi s O31u ValI As p 255 O31y O31n As p Pr a Thr 335 Le u Ph e <210O> 447 <21 1> 1231 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1231) <223> FRXA00956 <400> 447 gccggtgcag gcacgtgggc tggggcgaaa gacgccggcg cgctgctgaa aattttagcg Page 571 seqi i st car r ect ed. t xt accatctcca cattccatta ctaaaggttt aaataggatc at g Nib t 1 act gaa aaa gaa Thr 3 u Lys 3 u aac Asn ggc G31y aag Lys ggc G31y t gc Cys ttc Phe cag O31n at c l Ie gca Al a gac Asp 150 gcg Al a gt g Val t ac Tyr cgt Ar g gag O31u 230 ggc G31y ggc (31y ttc Phe ttc Phe t ac Tyr aac Asn aag Lys at c l Ie gca Al a 120 gcg Al a gcc Al a gt c Val gaa O31u ct c Leu 200 ttc Phe acc Thr aac Asn t cc Ser gcg Al a gac Asp cgc Ar g cca Pr o gaa G u cag O3n acc Thr at g lvb t cag O3n ccc Pr o 170 ct g Leu acc Thr aag Lys aag Lys at c l Ie 250 acg Thr gaa O31u gcg Al a gat Asp ct c Leu 75 gac Asp gt g Val ggc G31y ggc G31y cag O31n 155 gt g Val cgc Ar g ccc Pr o gt g Val ct t Leu 235 ggc O31y ct g Leu t cc Ser acc Thr t ac Tyr gca Al a ct c Leu ct g Leu gca Al a ct c Leu 140 ccc Pr o gaa O31u gat Asp gcc Al a at c l Ie 220 ccc Pr a at g lvb t cct Pr o ct g Leu agc Ser ggc O31y gaa O31u cac Hi s gcc Al a 110 cag O31n t gc Cys gt c Val ggt O31y acc Thr 190 ccg Pr a gag O31u gt t Val gca Al a gca Al a 15 cct Pr a cca Pr a cgc Ar g ggc O31y ggc (31y 95 aag Lys cac Hi s gt t Val t ac Tyr t cc Ser 175 gca Al a cac Hi s gaa O31u gt g Val gac Asp 255 t ac Tyr gct Al a gag O31u cca Pr a aaa Lys ggt G31y cgc Ar g ggc O31y gt c Val cgc Ar g 160 ggc O31y acc Thr cca Pr a gcc Al a gt c Val 240 ttc Phe ttc Phe ct c Leu ttc Phe acc Thr ggc O31y gca Al a at g lvb t acc Thr t ac Tyr 145 at g lvb t acc Thr ttc Phe ttc Phe aag Lys 225 gcc Al a at t l Ie ggt (31y gac Asp cgc Ar g ccg Pr a ttt Phe cac Hi s ggc O31y gcc Al a 130 at g lvb t cag O31n ct g Leu cac Hi s cca Pr a 210 gca Al a t gt Cys gac Asp gaa (31u cag O31n gaa O31u ct g Leu gcg Al a aaa Lys aaa Lys 115 acc Thr ggc O31y ct g Leu aag Lys gag O31u 195 acc Thr cag O31n gt c Val gat Asp ttc Phe ct g Leu gaa O31u acc Thr cgg Ar g act Thr 100 acc Thr gct Al a gcc Al a cac Hi s gac Asp 180 t cc Ser at c l Ie at g lvb t ggt O31y gaa 31u 260 Page 572 sec gta gag ctc gtc ggc gct gag cca g Val G31u Leu Val G31y Al a G31u Pr o P 265 2 aag cac ggc gca acc at c acc aac g Lys Hi s G31y Al a Thr Ilie Thr Asn C 280 285 acc cgt t cc t ac ct g at g cgc aac t Thr Ar g Ser Tyr Leu I'b t Ar g Asn S 295 300 t ac t cc at c t cc gcc gga ct t gat t Tyr Ser Ilie Ser Al a G31y Leu Asp T 310 315 gca cac ctg cac gcc acc ggc cgc g Al a Hi s Leu Hi s Al a Thr G31y Ar g P 330 gcc gaa gcc ctc caa gca ttc cag t Al a G3 u Al a Leu G3 n Al a Phe G3 n T 345 3 atc ccc gca ctg gaa tcc tca cac g Ilie Pr o Al a Leu G u Ser Ser Hi s P 360 365 gcc aag acc gcc Al a Lys Thr Al a 375 <210> 448 <211> 377 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 448 I'bt Thr G3 u Lys G3 u Asn Leu G3 y C 1 5 Phe a3 y a3 u Phe a3 y a3 y a3 n Phe\ Leu Asp a3 n Leu a3 u Lys Al a Phe\ 40 Phe Arg a31u a31u Leu a31y a31y Ty r L 55 Thr Pr o Leu Thr a31u Cy s Se r Asn L 70 a31y Phe Al a Ar g I Ie Phe Leu Lys P Al a Hi s Lys Thr As n a31n Va I lie C 100 1 I'b t a31y Lys Thr Ar g I Ie Ilie Al a C 115 120 Thr Al a Thr Al a Leu Al a Cys Al a L 130 135 Tyr k~bt (31y Al a Lys Asp Val Al a P 145 150 lIi st car r ect ed. t xt cc ggt gaa ggc ct C I a a3 y a3 u a3 y Leu 70 igt cag at c ggc at c y (31n I Ie (31y I Ie 290 cc gac ggc caa gt g ier Asp a3 y a3 n Val 305 ac cca ggc gt c ggc yr Pr o a31y Val a31y 320 cc acc t ac gt t ggt I a Thr Tyr Val a31y 335 ac ct C gcc cgc t ac yr Leu Al a Ar g Tyr ~50 cg ttc gcc tac gca Il a Phe Al a Tyr Al a 370 g ac As p 275 ct g Le u g aa a31u cca Pr o at c I Ie g aa a31u 355 ct c Le u t cc Ser C ac Hi s gag a31u C ag a31n ac c Thr 340 ggc aag Ly s 931 979 1 027 1 075 1123 1171 1219 1231 ~y Ser Thr Leu Leu Pro Al a Tyr 25 .eu .eu ~r g 05 .eu ~r g 10 Al a a3 u Se r As p Al a Thr Ar g As p Ty r Pr o Leu Al a a3 u Asp Leu 90 a31n Val Leu Thr a3 y Al a I~bt a31y Leu 140 O31n (31n Pr o 1 Page 573 Le u As n Le u a31y ValI Le u a31y 125 a31u As n Le u Ser a31y a31u Hi s Al a 110 a31n Cys ValI Pr o Pr o Ar g a31y a31y Hi s ValI Tyr seqI i st car r ect ed. t xt I'bt O n Leu Hi s Thr Ph e Ph e Ly s 225 A a I Ie O31y G y O31n 305 ValI ValI Ar g Tyr Ly s O31u 1 95 Thr O31n ValI As p As p 275 Le u (31u Pr o I Ie (31u 355 Le u G31y 1 65 A a Hi s ValI Le u G31y 245 G31y G31y G y Ser Hi s 325 As p I Ie Ar g A a Lys Val I Ie Pro Val O3 u Se r 03 y Se r 03 y 1 ValI Tyr Ar g (31u 230 G31y ValI Lys5 Thr Tyr 310 A a A a I Ie A a As n Le u (31u 215 Ar g Ser (31u Hi s Ar g 295 Ser Hi s (31u Pr o Lys5 375 (31u Le u 200 Ph e Thr As n Le u G31y 280 Ser I Ie Le u A a A a 360 Thr A a 1 85 G31y Hi s G31y A a ValI 265 A a Tyr Ser Hi s Le u 345 Le u A a Le u Thr Lys5 Lys5 I Ie 250 G31y Thr Le u A a A a 330 O31n (31u Ar g Pr o ValI Le u 235 O31y A a I Ie vb t O31y 315 Thr A a Ser Tr p O31y 205 Ser As p Ph e Pr o As n 285 As n As p Ar g O31n Hi s 365 Thr 1 Pr a O31u ValI A a A a 270 O31y Ser Tyr A a Tyr 350 A a A a Hi s O31u ValI As p 255 O31y O31n As p Pr a Thr 335 Le u Ph e <210O> 449 <21 1> 1401 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1378) <223> RXA00064 <400> 449 t cccct aaaa cgt ct agagt agt ggct tga ggt cact gct ct tt tt t tgt gccct tt tt t ggt ccgt ct a tt t tgccacc acat gcggag gt acgcagt t at g agt t ca gt t t cg I'b t Ser Ser Val Ser 1 ct g cag gat t tt gat gca gag cga att ggt t tg t tc cac gag gac at t Leu O31n Asp Phe Asp Al a 0u Arg Ile 03 y Leu Phe Hi s 03 u Asp Ilie 15 aag cgc aag ttt gat gag ctc aag tca aaa aat ct g aag ct g gat ct t Lys Arg Lys Phe Asp (31 u Leu Lys Ser Lys Asn Leu Lys Leu Asp Leu 30 Page 574 seql i st cor r ect ed. t xt act Thr ttg Leu gt c Val tgg Tr p gct Al a ttc Phe aag Lys gag Gu 150 cct Pr o ggc Gy aca Thr gac Asp gat Asp 230 ggc G y ct c Leu aag Lys gt c Val ggt Gy ttg Leu aac Asn gat Asp agc Ser aac Asn 120 at t SIle ttc Phe at g Met tgg Tr p gac Asp 200 cgc Ar g ttc Phe ccg Pr o ggt Sy t ac Tyr 280 cag G n cct tcg tcg Pro Ser Ser ggt 99t G y ggc G y ct g Leu aac Asn gat Asp cct Pr o ttt Phe gct Al a 170 gtt Val gca Al a gt g Val gag Su cgt Ar g 250 ggc G y ggc G y gct Al a ggt G y ct g Leu gtt Val at g Met gtt Val ccg Pr o 140 at g Met gag Gu gt g Val cgt Ar g gat Asp 220 at c SIle tgg Tr p tcg Ser gcg Al a gcg Al a 300 gag cag ttg G u G n Leu 45 gat ttc aag Asp Phe Lys gat ggc at c Asp G y II e cct gt g gag Pro Val G u 95 ttt gat gtg Phe Asp Val 110 cag cct tgg G n Pro Trp 125 ggc tat gat G y Tyr Asp att tct gtg IIe Ser Val gaa ttg gtg G u Leu Val 175 ttt tct aac Phe Ser Asn 190 ct a agc gca Leu Ser Al a 205 aat gcc t ac Asn Al a Tyr gat atc gt c Asp Ile Val gcg ttc act Al a Phe Thr 255 ttc ttc ctc Phe Phe Leu 270 ggt at c cgt Sy lie Ar g 285 cgt tac ttt Arg Tyr Phe gat Asp gct Al a gtt Val cag G n at c SIle tcg Ser cgc Ar g cca Pr o 160 aag Lys ccg Pr o at g Met gcc Al a ggg G y 240 tct Ser acc Thr ggc G y ttc gct Phe Al a gcg gat Al a Asp gat at t Asp II e gtc ttg Val Leu agc tgg Ser Trp aag gaa Lys G u 130 cat ttc Hi s Phe 145 at g aat IVet Asn aat ccg Asn Pro act ggt Thr G y gaa acc G u Thr 210 gtt cat Val Hi s 225 ctt ggt Leu G y act tcg Thr Ser tct gcg Ser Al a att ggc I le y 290 gat Asp ggt G y cgc Ar g gcg Al a tcg Ser 115 gaa G u tcc Ser gaa G u cag G n ttc Phe 195 gca Al a acg Thr gag G u aag Lys gag G u 275 cct Pr o gag G u act Thr cag G n ggg G y 100 t ac Tyr acc Thr at c SIe gac Asp gtt Val 180 acg Thr gct Al a ct g Leu gcc Al a at c SIe 260 aac Asn aac Asn 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 ggc gat G y Asp 305 gct gag gga gtg Al a u G y Val Page 575 cgc Ar g 310 aag Lys cag G n cct Pr o gcg Al a aac Asn 390 gag ggu gag Gu gt g Val ct g Leu act Thr acg Thr 360 acg Thr aat Asn gcc Al a t ac Tyr cgt Ar g at t SIle 330 cct Pr o tct Ser gcg Al a cgt Ar g gat Asp 410 aac Asn seql i st cor r ect ed. t xt aag cat gct gcg tcg ttg gct ccg Lys His Ala Ala Ser Leu Ala Pro 315 320 ctg gat tct cgc ctt gct gag tac Leu Asp Ser Arg Leu Ala G u Tyr 335 gcg ggc ggt tac ttc att tcc ctt Ala Gy G y Tyr Phe lie Ser Leu 350 cgc gtg gct gag ttg gct aag gaa Arg Val Ala G u Leu Ala Lys G u 365 370 ggt tct tct tac ccg ctg cgt cag G y Ser Ser Tyr Pro Leu Arg G n 380 385 ttg gca ccg tcg ctg cct cca gtt Leu Ala Pro Ser Leu Pro Pro Val 395 400 ggc gtg gct acc tgt gtg ctg ttg G y Val Ala Thr Cys Val Leu Leu 415 taaaagtgaa tacagcggag aca aag Lys ggt Sy gat Asp 355 gcc Al a gat Asp gag G u gca Al a ttc Phe gt c Val 340 gt g Val ggc G y ccg Pr o gaa G u gca Al a 420 1075 1123 1171 1219 1267 1315 1363 1401 <210> 450 <211> 426 <212> PRT <213> Corynebacter i um gl ut am cum <400> 450 IVet Ser Ser Val Ser Leu G n Asp Phe 1 5 Phe His u Asp Ile Lys Arg Lys Phe Leu Lys Leu Asp Leu Thr Arg Gy Lys 40 Phe Ala Asp G u Leu Leu Ala Leu Pro 55 Ala Asp y Thr Asp Val Arg Asn Tyr 70 Asp lie Arg n lie Trp Ala Asp Leu Val Leu Ala y Asp Al a Ser Ser Leu 100 105 Ser Trp Ser Tyr Ile Phe y Asn Asn 115 120 Lys G u G u Thr Val Lys Trp lie Cys 130 135 His Phe Ser lie Thr G u Arg Phe G y Asp Ala G u Arg 10 Asp G u Leu Lys Pro Ser Ser G u G y Lys G y Asp G y G y Leu Asp Leu G y Val Pr o 90 Asn Ile IVet Phe Asp Ser Val G n 125 Pro Val Pro G y 140 Phe u 'e t lie Page 576 Sy Lys Leu Lys SIe G u Val Tr p Asp Val seqI i st car r ect ed. t xt 150 Pr o Asp I'bt vb t As n Thr O31u ValI 225 Le u Thr Ser I Ie As p 305 A a O31u Ser Ly s Ar g 385 Pr o Le u O31u (31n Ph e 1 95 A a Thr O31u Ly s O31u 275 Pr o O31u Ly s G31y As p 355 A a As p O31u A a G31y 1 65 Ly s ValI Pr o Thr A a 245 Thr Ar g Ly s ValI As n 325 A a ValI I Ie O31u Le u 405 A a G31y Thr As p As p 230 G31y Le u Ly s ValI Ar g 310 Ly s O31n Pr o A a As n 390 G31u (31u G31u Ph e 215 G31u As n A a Tr p As n 295 A a ValI Tr p G31y Le u 375 Ly s ValI Hi s Tr p As p 200 Ar g Ph e Pr o G31y Tyr 280 O31n ValI Le u Thr Thr 360 Thr As n A a Tyr 1 Asp A a Val 1 70 Val Val Pr o 1 Val A a Lys Val Val Tr p Pro O31u Val 235 Asn Arg Phe 250 A a 03 y Val 265 Thr 03 y Hi s Leu A a Hi s I'b t Ar g Lys 315 O3 u Il e Leu 330 Val Pr o Ala 345 Ala Ser Ar g O3 y A a 03 y Leu Arg Leu 395 [vb t Asp (1y 410 A a Asn 425 03 u 03 u Leu ValI Ar g As p 220 li1e Tr p Ser A a A a 300 Hi s As p G31y ValI Ser 380 A a ValI Ph e Le u 205 As n As p A a Ph e G31y 285 Ar g A a Ser G31y A a 365 Ser Pr a A a ValI 1 As n A a Tyr ValI Thr 255 Le u Ar g Ph e Ser Le u 335 Ph e Le u Pr a Le u cys 415 <210O> 451 <21 1> 1143 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (1120) <223> RXN00448 <400> 451 ccat tt acct t caact aagg t agccgt aac t gcaaagct c aggccat cct ct t cagt gt t cat agagat a accgt agt ag gt at gt gcca cact t gt cag gt g act acc aaa gac 115 Page 577 seql i st cor r ect ed. t xt Val Thr Thr Lys Asp at t l Ie ct c Leu cgc Ar g t cc Ser ct c Leu gcc Al a gt a Val cgt Ar g agc Ser ttc Phe 150 at c l Ie gt c Val cat Hi s ggt Gy acc Thr 230 agc Ser cgc Ar g cgc Ar g cgc Ar g gat Asp gt c Val cac Hi s acc Thr gt g Val 120 t cc Ser cag G n aaa Lys t cc Ser aca Thr 200 gca Al a gtt Val gcc Al a gt a Val ct c Leu ggc Gy gaa G u gcg Al a cac Hi s gt c Val t cc Ser gac Asp ttc Phe gt c Val 170 gtt Val at c SIle tct Ser ggc Gy cca Pr o 250 tgc Cys cat Hi s gct Al a gca Al a gt c Val 75 gca Al a tac Tyr cac Hi s gga G y gac Asp 155 gt c Val ggc Gy ct g Leu ct c Leu acc Thr 235 ct g Leu at c SIle gca Al a aaa Lys acc Thr ccc Pr o cca Pr o gac Asp ccc Pr o ct g Leu 140 ggc G y caa G n cca Pr o gct Al a tct Ser 220 gac Asp gt c Val ct g Leu gcc Al a t ca Ser 45 ct c Leu at g Met aac Asn gca Al a at g Met 125 ttc Phe acc Thr at g Met cac Hi s gaa G u 205 tta Leu cca Pr o aaa Lys ggc G y aac Asn gcc Al a cag G n acc Thr aac Asn gt a Val 110 gca Al a aaa Lys gac Asp gca Al a gat Asp 190 acc Thr gcc Al a gga Gy gcc Al a ct c Leu 15 cac Hi s gt c Val cgt Ar g gca Al a ggc Gy 95 aaa Lys ggc G y cga Ar g at c SIle ct c Leu 175 gca Al a ct c Leu gct Al a ct c Leu ct c Leu 255 ggc G y t cc Ser gac Asp gca Al a at c l Ie ttc Phe gcc Al a acc Thr gca Al a aac Asn 160 gcc Al a gca Al a gcc Al a ggc Gy gt c Val 240 gac Asp ct c Leu gt c Val gaa G u gcc Al a gat Asp acc Thr cgc Ar g gcc Al a gt a Val 145 t cc Ser gt g Val gca Al a at c l Ie agc Ser 225 cgc Ar g gaa G u at c SIle ttc Phe ggc Gy gcc Al a tcg Ser gac Asp aac Asn aac Asn 130 tgg Tr p acc Thr ggc G y gca Al a gt c Val 210 t ac Tyr gcc Al a gca Al a ggc G y ggc Gy ttc Phe gaa G u ctt Leu gt c Val at g Met 115 t cc Ser gt g Val tgg Tr p gct Al a cga Ar g 195 ggt Gy cgc Ar g at g Met ct g Leu gga G y t ac Tyr gac Asp gat Asp ct c Leu gta Val 100 caa G n ggc G y gtc Val at c SIe gaa G u 180 gtg Val gac Asp gac Asp tgt Cys gcg Al a 260 ctc cac gaa gcc cgc gaa ggc ctc acc gca gaa cag cca aac atc gag Page 578 seqI i st car r ect ed. t xt Leu Hi s O31u Al a Ar g O31u O31y L eu Thr Ala Ou On Pro 265 270 caa ctt gcc gac aac ggc tac cga tcc cgc atc cgc tac O31n Leu Al a Asp Asn G31y Tyr Ar g Ser Ar g I Ie Ar g Tyr 280 285 290 tcc ggc cag cga cgc gcc aaa gaa tcc gtt agc cct acc Ser G31y O31n Ar g Ar g Al a Lys O31u Ser Val Ser Pr o Thr 295 300 305 t cc agg cca gt g ct C cgt ct C cac ccg ggc aca cca aac Ser Ar g Pr o Val Leu Ar g Leu Hi s Pr o G31y Thr Pr o Asn 310 315 320 cag ct c at c cac gct gaa acc ct C ggc gca cgg at c gaa O31n Leu Ilie Hi s Al a (1u Thr Leu G31y Al a Arg Ilie O31u 330 335 t agtt t tat c ggct gat gat t ct <210> 452 <211> 340 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ami cumn As n 275 g aa O31u at c I Ie t gg Tr p gt g ValI I Ie gcc Al a ac c Thr gag O31u tt c Ph e 340 979 1 027 1 075 1120 1143 <400> 452 Val Thr Thr Le u ValI O31u Al a As p Thr Ar g Al a ValI 145 Ser ValI Al a G31y G31y Ph e O31u Le u ValI vb t 115 Ser ValI Tr p Al a Ar g 1 95 Lys Asp 03 y Se r Ty r As n As p Val As p Al a Leu Asp Val Ser 1 00 O3 n Hi s G31y Tr p Val Thr I I e Ser 1 65 O3 u Val 1 80 Val Ser I I e Se r Ar Leu Leu Ar Ar g Ser Ar 4 Ser Al a As Leu Il e Va 70 Al a Val Hi Val Lys Th Ar g Tyr Va 12 Ser Al a Se 135 Phe Asp 03 150 Ilie Tr p Ly Val Pr o Se Hi s Leu Th 20 Pr o As p 25 Ser Le u Le u Thr Al a 1 05 G31y vb t Le u As p Ar g 1 85 Hi s cys Hi s Al a Al a ValI 75 Al a Tyr Hi s G31y As p 1 55 ValI G31y Le u I Ie Al a Lys5 Thr Pr o Pr o As p Pr a Le u 140 G31y O31n Pr a Al a Le u Al a Ser Le u vb t As n Al a vb t 125 Ph e Thr vb t Hi s O31u 205 G31y As n Al a O31n Thr As n ValI 110 Al a Lys5 As p Al a As p 1 Thr Le u Hi s ValI Ar g Al a G31y Lys5 G31y Ar g I Ie Le u 1 Al a Le u G31y Ser As p Al a I Ie Ph e Al a Thr Al a As n 1 Al a Al a Al a IlieVal Gy AspAsn OyOy AlaLeu Ser Leu Ser Leu Al a Al a G31y Page 579 Ser 225 Ar g (31u O31n Ar g Pr o 305 Pr o I Ie Ar g Le u As n 275 (31u I Ie Tr p ValI Ser G31u 245 li1e (31u Ar g Ser Ly s 325 215 Ar g As n Hi s Le u G31y 295 Ar g Le u seqi i st car r ect ed. t xt 220 Val Ala G31y Thr Asp Pr o 235 Ala G31y Pr o Leu Val Lys 250 O31u Ala Ar g 31u G31y Leu 265 Ala As p Asn G31y Tyr Ar g 280 285 O31n Ar g Ar g Ala Lys O31u 300 Pr o Val Leu Ar g Leu Hi s 315 li1e Hi s Ala O31u Thr Leu 330 G31y A a Thr 270 Ser Ser Pr o G31y Le u Le u 255 A a Ar g ValI G31y A a 335 <210O> 453 <21 1> 689 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (666) <223> FRXA00448 <400> 453 t at gtg gga Ty r Val G31y gca t cc at g A a Ser l'b t gac cag ctt Asp 03 n Leu tgg aaa gac Trp Lys Asp cca t cc cga Pr o Ser Ar g tta aca cac Leu Thr Hi s ggc gca ct g 03 y A a Leu cgc gt t gca Ar g Val Ala c ac Hi s gga G31y g ac As p gt c ValI G31y ct g Le u ct c Le u ac c Thr CCC Pr o ct g Le u ggc G31y caa O31n cca Pr a 70 gct A a t ct Ser gac As p at g vb t tt c Ph e ac c Thr at g vb t c ac Hi s g aa (31u tt a Le u cca Pr a gca A a aaa Lys5 g ac As p 40 gca A a g at As p ac c Thr gcc A a gga G31y ggc G31y cga Ar g at c I Ie ct c Le u gca A a ct c Le u gct A a 1 Ct C Le u ac c Thr 10 gca A a aac As n gcc A a gca A a gcc A a 90 ggc G31y gcc A a gt a ValI t cc Ser gt g ValI gca A a at c I Ie ag c Ser t cc Ser gt g ValI t gg Tr p gct A a cga Ar g ggt G31y cgc Ar g t gg Tr p ac c Thr ag c Ser gt t ValI t ct Ser aac As n t cc Se r gt c cgc gcc at g t gt gaa agc Val Arg Ala l'bt Cys Ou Ser Page 580 seqI i st car r ect ed. t xt 115 120 125 aac gcc ggc cca ct g gt c aaa gcc ct C gac gaa gca ct g Asn Al a G31y Pro Leu Val Lys Al a Leu Asp O31u Al a Leu 130 135 140 cac gaa gcc cgc gaa ggc ctc acc gca gaa cag cca aac His O31u Al a Ar g O31u O31y Leu Thr Al a O31u O31n Pr o Asn 145 150 155 ctt gcc gac aac ggc tac cga tcc cgc atc cgc tac gaa Leu Al a Asp Asn G31y Tyr Ar g Ser Ar g Ilie Ar g Tyr (31u 165 170 ggc cag cga cgc gcc aaa gaa tcc gtt agc cct acc atc G31y O31n Ar g Ar g Al a Lys O31u Ser Val Ser Pr o Thr Ilie 180 185 agg cca gt g ct C cgt ct C cac ccg ggc aca cca aac t gg Ar g Pr o Val Leu Ar g Leu Hi s Pr o G31y Thr Pr o Asn Tr p 195 200 205 ctc atc cac gct gaa acc ctc ggc gca cgg atc gaa gtg Leu Ilie Hi s Al a (1u Thr Leu G31y Al a Arg Ilie O31u Val 210 215 220 t agtt t tat c ggct gat gat t ct <210> 454 <211> 222 <212> PRT <213> Cor ynebact er i urn gi ut ani cumn gcg Al a at c I Ie gcc Al a ac c Thr 1 gag O31u tt c Ph e at c I Ie gag O31u cgc Ar g 1 t ca Ser aag <400> 454 Tyr Al a As p Tr p Pr o Le u G31y Ar g As n Hi s 145 Val G31y Se r Ser I'b t As r 2 C (3 n Leu PhE Lys Asp Val Ser Ar g Val Thr Hi s I E Al a Leu Ser 1 OC Val Al a (31 115 Ala G31y Pr c 130 (31u Al a Ar c Hi s Pr o O3 y Leu As p (1y Val 03 n G31y Pr o 70 Leu Al a Leu Ser Thr As p Leu Val O31u (1y 150 vb t Ph e Thr vb t 55 Hi s O31u Le u Pr o Lys5 135 Le u Al a Lys5 As p 40 Al a As p Thr Al a G31y 120 Al a Thr G31y Ar g 25 li1e Le u Al a Le u Al a 1 05 Le u Le u Al a Thr 10 Al a As n Al a Al a Al a 90 G31y ValI As p Ou le 1 70 Al a ValI Ser ValI Al a 75 li1e Ser Ar g O31u O31n 1 55 As n Tr p Thr G31y Al a ValI Tyr Al a Al a 140 Pr o Ser ValI Tr p Al a Ar g G31y Ar g vb t 125 Le u As n G31y ValI li1e O31u ValI As p As p 110 cys Al a I Ie Tr p Ser Thr Phe Ser I Ie Val Val Ser Hi s As n (1y Ser Thr O3 u Ser I I e Leu G31u (1n 1 Ar g Ser 1 Leu Al a Asp Asn G31y 1 65 Tyr Arg Ser Arg Ar g Tyr O31u Al a Page 581 seql i st cor r ect ed. t xt Oy n Arg Arg Ala Lys u Ser Val Ser Pro Thr lie Thr Ser Ser 180 185 190 Arg Pro Val Leu Arg Leu His Pro G y Thr Pro Asn Trp G u Lys G n 195 200 205 Leu lie His Ala G u Thr Leu G y Ala Arg I I e G u Val Phe 210 215 220 <210> 455 <211> 346 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(346) <223> FRXA00452 <400> 455 ccatttacct tcaactaagg tagccgtaac tgcaaagctc aggccatcct cttcagtg catagagata accgtagtag gtatgtgcca cact t gtcag gtg act acc aaa gac tt att tcc cgc cca gta tgc Ile Ser Arg Pro Val Cys ct c ctc cgc gac ctc cat Leu Leu Arg Asp Leu His cgc tca cgc tcc ggc gct Arg Ser Arg Ser Gy Ala tcc gcc gat ctt gaa gca Ser Ala Asp Leu G u Al a ctc atc gtc ctc gcg gt c Leu lie Val Leu Ala Val 75 <210> 456 <211> 82 <212> PRT <213> Corynebacter i um gl <400> 456 Val Thr Thr Lys Asp II e 1 5 Leu lie Gy y Ser Leu Val Phe y Tyr Asn Arg G u y Phe Asp Val Ser Al a Al a G u Asp Al a Leu 70 at c SIle gca Al a aaa Lys acc Thr 60 ccc Pr o ct g Leu gcc Al a t ca Ser 45 ct c Leu at g Met ggc G y aac Asn 30 gcc Al a cag G n acc Thr ct c Leu 15 cac Hi s gt c Val cgt Ar g gca Al a ggc G y t cc Ser gac Asp gca Al a at c l Ie Val Thr Thr Lys Asp 1 ctc atc ggc gga tcc Leu lie G y G y Ser gtc ttc ggc tac aac Val Phe G y Tyr Asn gaa ggc ttc gac gtt G u G y Phe Asp Val gcc gcc gaa gat gcg Ala Ala G u Asp Ala gat tcg Asp Ser ut am cum Ser Arg Pro Leu Arg Asp 25 Ser Arg Ser 40 Al a Asp Leu 55 Ile Val Leu Val Cys Ile Leu Gy Leu Gy 10 Leu His Ala Ala Asn His Ser Sy Ala Lys Ser Ala Val Asp G u Ala Thr Leu n Arg Ala Al a Val Pro M\t Thr Ala lle 75 Page 582 seql i st cor r ect ed. t xt Asp Ser <210> 457 <211> 1248 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1225) <223> RXA00584 <400> 457 tagttgtgcc acctaaaacg cgaacagaac cggagtcgag agaggggctg cttttttgtt tcctaaattc accccatccc agg Ar g acc Thr gat Asp gag G u cgc Ar g gcc Al a cag G n at c SIle t ac Tyr gtt Val 150 agc Ser cgt Ar g gaa G u aac Asn at c SIle gat Asp gtt Val gat Asp ct c Leu gga Sy 120 at c SIle aac Asn cag G n acc Thr at g Met cgc Ar g gca Al a cag G n gtt Val gca Al a at t SIle aag Lys gag G u gat Asp t ac Tyr 170 tct Ser agt Ser gt c Val aac Asn gac Asp gt g Val 75 aac Asn gt c Val gga Gy ggc Gy ct c Leu 155 gcc Al a cag G n tct Ser gt g Val cca Pr o at c SIle gga G y cgc Ar g at g Met ttg Leu ttg Leu 140 cca Pr o gac Asp gt g Val cca Pr o gct Al a ct g Leu 45 gct Al a cct Pr o ct g Leu cgc Ar g at c SIle 125 cgc Ar g gt c Val act Thr cac Hi s gtc t ca Val Ser 15 ttc cac Phe Hi s aca cca Thr Pro gat at c Asp II e tgc t ca Cys Ser gct ccg Ala Pro 95 gtg tac Val Tyr 110 aat gat Asn Asp att gcg I e Al a ggc tgc Sy Cys gtc gca Val Ala 175 cgc cag Arg G n ct c Leu gag G u aag Lys ttc Phe gtt Val 80 ct g Leu ttc Phe cct Pr o cgc Ar g gaa G u 160 tgg Tr p cagcacctcc ccgcaagggt atg cat agc cct gaa 11! Met His Ser Pro G u 1 gaa aac gcg gcg tca 16 G u Asn Ala Ala Ser ctg cct agc cct aca 21 Leu Pro Ser Pro Thr cag gct tcc aag gtg G n Ala Ser Lys Val gct ggc gac gat gac Al a y Asp Asp Asp cac gat cct gaa gca His Asp Pro G u Ala gca aag cgc ctt gat Ala Lys Arg Leu Asp 100 gag aag cct cgc acc G u Lys Pro Arg Thr 115 cac ctc aac gaa acc 49! His Leu Asn G u Thr 130 aaa gtg ctt atc gac 54 Lys Val Leu Ile Asp 145 ttc ctc gaa cca aac 59! Phe Leu G u Pro Asn 165 gga gca atc ggc gct 64; Sy Ala lle y Ala 180 ctg gct tct ggg atg tct Leu Ala Ser y et Ser Page 583 seql i st cor r ect ed. t xt 185 190 195 atg cca att ggt ttc aag aac gga act gac gga aac atc cag gtt gca 739 IVet Pro lie y Phe Lys Asn y Thr Asp y Asn Ile n Val Ala 200 205 210 gtc gac gcg gta cag gct gcc cag aac cca cac ttc ttc ttc gga acc 787 Val Asp Ala Val G n Al a Ala G n Asn Pro His Phe Phe Phe G y Thr 215 220 225 tcc gac gac ggc gcg ctg agc gtc gtg gag acc gca ggc aac agc aac 835 Ser Asp Asp y Ala Leu Ser Val Val G u Thr Ala y Asn Ser Asn 230 235 240 245 tcc cac atc att ttg cgc ggc ggt acc tcc ggc ccg aat cat gat gca 883 Ser His lie lie Leu Arg G y G y Thr Ser G y Pro Asn His Asp Ala 250 255 260 gct tcg gtg gag gcc gtc gtc gag aag ctt ggt gaa aac gct cgt ctc 931 Ala Ser Val G u Ala Val Val G u Lys Leu Gy G u Asn Ala Arg Leu 265 270 275 atg atc gat gct tcc cat gct aac tcc ggc aag gat cat at c cga cag 979 IVet IIe Asp Ala Ser Hi s Ala Asn Ser G y Lys Asp His Ile Arg G n 280 285 290 gtt gag gtt gtt cgt gaa atc gca gag cag att tct ggc ggt tct gaa 1027 Val u Val Val Arg u Ile Ala u n Ile Ser Gy y Ser u 295 300 305 gct gtg gct gga atc atg att gag tcc ttc ctc gtt ggt ggc gca cag 1075 Ala Val Al a Gy Ile IVet I e Gu Ser Phe Leu Val y G y Al a n 310 315 320 325 aac ctt gat cct gcg aaa ttg cgc atc aat ggc ggt gaa ggc ctg gtg 1123 Asn Leu Asp Pro Ala Lys Leu Arg lie Asn Gy Gy G u y Leu Val 330 335 340 tac gga cag tct gtg acc gat aag tgc atc gat att gac acc acc atc 1171 Tyr Gy G n Ser Val Thr Asp Lys Cys Ile Asp Ile Asp Thr Thr Ile 345 350 355 gat ttg ctc gct gag ctg gcc gca gca gta agg gaa cgc cga gca gca 1219 Asp Leu Leu Ala G u Leu Ala Ala Ala Val Arg G u Arg Arg Ala Ala 360 365 370 gcc aag taattaaggg cgctagactg tta 1248 Ala Lys 375 <210> 458 <211> 375 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 458 IVet His Ser Pro G u Arg n G u Lys IVet Ser Ser Pro Val Ser Leu 1 5 10 G u Asn Ala Ala Ser Thr Ser Asn Lys Arg Val Val Ala Phe His u 25 Leu Pro Ser Pro Thr Asp Leu lie Ala Ala Asn Pro Leu Thr Pro Lys 40 G n Ala Ser Lys Val G u G n Asp Arg G n Asp lie Ala Asp Ile Phe 55 Page 584 seql i st cor r ect ed. t xt Al a Hi s Al a G u Hi s Lys 145 Phe Sy Al a Asn Phe 225 Al a Pr o G u Asp Ser 305 Val G y lie Gu G y Asp Asp Asp Ar g Leu Val Val Val Val y Pro Cys Ser Val Pr o Ar g Pr o 115 Asn Leu Su I e Gy 195 G n Phe Asn Hi s Al a 275 I le Sy Sy Sy Thr 355 Ar g Al a Asp Thr Thr Asp Asn 165 Al a Ser Al a Thr Asn 245 Al a Leu G n G u G n 325 Val SIle Al a Al a G n SIle Tyr Val 150 Ser Ar g bet Val Ser 230 Ser Al a bet Val Al a 310 Asn Tyr Asp Al a I e Asp Asp Leu Val G y 120 Asp Il e 135 Val Asn Pro G n Thr Thr Pro Ile 200 Asp Al a 215 Asp Asp His Il e Ser Val IIe Asp 280 G u Val 295 Val Ala Leu Asp G y G n Leu Leu 360 Lys 375 Tyr Lys 105 Tr p Pr o Leu Tyr G u 185 Gy Val Gy I e Su 265 Al a Val Sy Pr o Ser 345 Al a 90 I e Lys G u Asp Tyr 170 Ser Phe G n Al a Leu 250 Al a Ser Ar g SIle Al a 330 Val Asn Val Sy Sy Leu 155 Al a G n Lys Al a Leu 235 Ar g Val Hi s G u et 315 Lys Thr Leu Ar g SIle 125 Ar g Val Thr Hi s Gy 205 G n Val Gy Su Asn 285 Al a G u Ar g Lys Al a Val 110 Asn SIle Sy Val Ar g 190 Thr Asn Val Thr Lys 270 Ser G u Ser SIle Cys 350 Pr o Tyr Asp Al a Cys Al a 175 G n Asp Pr o G u Ser 255 Leu Sy G n Phe Asn 335 I e Ala u Leu Ala Ala Ala Val Arg 365 <210> 459 <211> 1983 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> Page 585 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(1960) <223> RXA00579 <400> 459 tcgtctaagt ttttctttga gttttcatat gtagaaggca gtgcttttct cgttgttttg tggttttgtc agaggatgtc tcgtcggctt cggcctggcg at t l Ie gag G u gat Asp gcc Al a gca Al a ttg Leu gag G u cct Pr a ttg Leu at g let 150 ccg Pr a ctt Leu ccg Pr a t cc Ser aat Asn acg Thr at g Met gtt Val gtt Val t at Tyr tcg Ser acg Thr 120 gag G u ttg Leu tct Ser tta Leu agc Ser 200 cat Hi s gat Asp cag G n ttc Phe gct Al a at t SIle ggt Sy at c SIle gag Su ttg Leu cat Hi s ggt G y 170 cgc Ar g gat Asp ttt Phe tct Ser gca Al a gac Asp gat Asp tt g Leu 75 gat Asp acc Thr gcg Al a aaa Lys gaa G u 155 gga G y aca Thr t ca Ser tgg Tr p ttc Phe cct Pr a gcc Al a ttt Phe ggt G y gtt Val cat Hi s gt g Val gct Al a 140 gt g Val caa G n t at Tyr gca Al a ct c Leu 220 acg Thr gt g Val gt c Val 45 ggt G y gt g Val gat Asp gat Asp cgt Ar g 125 aca Thr ctt Leu ttc Phe cgc Ar g gcg Al a 205 gat Asp ttt Phe gt g Val at c SIle at c SIle tgt Cys ttg Leu ggt G y 110 t at Tyr gct Al a ccg Pr a ggc G y tgg Tr p 190 gtt Val gat Asp aat Asn 15 gt g Val ct c Leu tgt Cys tta Leu gcg Al a 95 t ca Ser cac Hi s acc Thr cag G n cat Hi s 175 caa G n ttt Phe gcc Al a ct c Leu cct Pr a t ca Ser gca Al a ggc G y 80 ccc Pr a ggt G y tcg Ser agc Ser tgg Tr p 160 cag G n ct c Leu gaa G u caa G n at g let 1 gcc Al a aat Asn cct Pr a ggc G y cac Hi s agg Ar g tta Leu at g Met gat Asp 145 ggt G y at c SIle acg Thr aca Thr gga G5y 225 cgc gtt Arg Val acc Thr gat Asp ggc gt c Val cag G n ccg Pr a ttt Phe gt g Val 130 gat Asp gt g Val at t SIle gag G u ttc Phe 210 acc Thr t at Tyr caa G n ccg Pr a at t SIle ggc G y gt c Val gca Al a 115 gca Al a ggt G y caa G n aag Lys aaa Lys 195 ttt Phe agc Ser tta att Leu II e gtg gaa Val G u gaa at a Su lle ggc cac G y Hi s gag cgt G u Arg att gcg I e Al a cac ggt Hi s y 100 ggc at c G y lie acc cgc Thr Arg ttg atc Leu II e ttt cat Phe Hi s 165 aac ttc Asn Phe 180 act att Thr Ile gcc cat Al a Hi s tat ctt Tyr Leu ggt gat gcc agc ggt cct ctc gca cgc aca aaa acc cat aat gtc ggc Page 586 O31y 230 gag O31u gt t ValI t at Tyr ct t Le u t cg Ser 310 g aa O31u gcg A a ct c Le u t at Tyr cct Pr o 390 ggt O31y gag O31u at t I Ie at c I Ie at g vb t 470 ac c Thr Asp Ala Ser O31y Pro Leu A 235 seqi i st car r ect ed. t xt a Arg Thr Lys Thr Hi 240 s As n Val O31y 245 g at As p ccc Pr o ct t Le u 280 g at As p c ag O31n ac c Thr gga O31y aaa Ly s 360 at c I Ie gct A a t at Tyr tt c Ph e ggc O31y 440 gct A a gt g ValI gt t ValI tt c Ph e 250 c aa O31n gcg A a c ac Hi s cgg Ar g aag Ly s 330 ct c Le u cgc Ar g ct g Le u t at Tyr c ag O31n 410 ac c Thr ag g Ar g ct g Le u tt g Le u ac a Thr 490 acc Thr ggt G31y gaa O31u ct c Le u t t g Le u 315 aag Ly s gct A a ag a Ar g acc Thr ct a Le u 395 ct g Le u at t I Ie ccg Pr o cgc Ar g gt c ValI 475 t cc Ser t gg Tr p tt t Ph e gct A a at t I Ie 300 ct g Le u ct g Le u tt g Le u gcc A a ac a Thr 380 gca A a ggg G31y g at As p cgt Ar g agt Ser 460 cgc Ar g aag Ly s ct a Le u cgt Ar g ggc G31y 285 tt t Ph e gcg A a cat Hi s c ag O31n c ag O31n 365 aaa Ly s ct g Le u g at As p t cg Ser ggg O31y 445 aat As n aac As n ct t Le u aag Ly s ct t Le u 270 gca A a gcc A a tt g Le u aat As n gt t ValI 350 gag O31u ct t Le u cgt Ar g ac c Thr gca A a 430 cga Ar g cct Pr o g ac As p tt c Ph e gag O31u 255 ggc O31y cgg Ar g g at As p ggg O31y ct t Le u 335 cga Ar g ct g Le u c ag O31n ggg O31y t ct Ser 415 ggg O31y ac a Thr aaa Ly s tt a Le u g ac As p 495 g at As p t gg Tr p gct A a cgc Ar g gag O31u 320 gt c ValI g at As p at t I Ie ggc O31y gcc A a 400 at t I Ie t at Tyr gcg A a g at As p gcc A a 480 gt c ValI ct c Le u gt t ValI gcg A a gcc A a 305 c ag O31n gcc A a t cc Ser act Thr ac c Thr 385 aat As n tt g Le u gt g ValI c aa O31n cgt Ar g 465 cgc Ar g g aa O31u gcc A a ggt O31y c ac Hi s 290 at c I Ie g ac As p ccg Pr a aaa Ly s cgc Ar g 370 act Thr ccc Pr a agt Ser g aa O31u g aa O31u 450 gca A a ggc O31y ac c Thr gcc A a t ac Tyr 275 act Thr gca A a gag O31u Ar g g at As p 355 ggc O31y g at As p ac c Thr t cc Ser t ca Ser 435 g ac As p g aa O31u gct A a t ac Tyr aac As n 260 gt t ValI t cg Ser gt g ValI t gg Tr p at a I Ie 340 gag O31u g aa O31u gt g ValI gca A a t cg Ser 420 aag Ly s c aa O31n aac As n tt g Le u gcc A a 500 t ca Ser ggt O31y agt Ser g aa O31u tt t Ph e 325 cct Pr a t at Tyr t cg Ser gcc A a t at Tyr 405 ccg Pr a ccc Pr a g aa O31u tt g Le u ccc Pr a 485 ac a Thr 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 1 603 1651 gt c cac caa ct t gt c agc acc gt c t ct gca gag t tg ggg cca cgc agt Page 587 seqIi ValI Hi s G3 n Leu Val Ser Thr Val Ser 505 510 ccg at t gag t gc gt g cgc gca gca tt c Pr o IIe O31u Cys Val Ar g Ala Ala Phe 520 525 gcc cca aag ctg cgc acc atg gag atc Ala Pr o Lys Leu Ar g Thr 'b t O31u Ilie 535 540 cct cgc ggt at t t ac t ca ggt ggc tt g Pr o Ar g O31y Ilie Tyr Ser O31y O31y Leu 550 555 gca gt t gat ct c t cc at g gt g at c aga Ala Val Asp Leu Ser I'b t Val Ilie Ar g 570 cac gt g gag t ac gga gt g ggc ggt gca Hi s Val O31u Tyr O31y Val O31y O31y Ala 585 590 gag gct gag t gg gag gaa at c cgc gt t O31u Ala O31u Tr p O31u O31u Ilie Ar g Val 600 605 t tg t tt ggg gt t gaa t tc cca t gacgt a Leu Phe G31y Val O31u Phe Pr o 615 620 <210> 460 <211> 620 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 460 I'bt Ar g Val Leu I Ie I Ie Asp Asn Tyr 1 5 Ala Th r Tyr Val 31u O31u ValI Thr G31y 25 Asn Asp O31n O31u IIe As p 31u I'b t Leu 40 Pro G31y Pr o G31y Hi s Ala G31y Val Ala 55 G31y Va I lie (31u Ar g Ala Ar g Val Pr o 70 Hi s a3 n G3 y Il e A a Leu A a Tyr G3 y Ar g Pr o Val Hi s 31y O31u ValI Ser O31n 100 105 Leu Phe Ala O31y I Ie Pro O31u Thr Phe 115 120 I'b t Val Ala Thr Arg Leu Pr o O31u Ser 130 135 Asp Asp 03 y Leu I I e I'b t A a Leu A a 145 150 st cor r ect ed. t xt Ala O31u L eu O31y Pr o 515 ccc ggt ggt t cg at g Pr o 31y O31y Ser I'b t 530 at c gat gag ct g gag I I e As p 03 u Leu 03 u 545 gga t at t tt t cc ct c 03 y Ty r Phe Ser Leu 560 act ct c gt c at c cag Thr Leu Val I Ie O31n 575 ct t ct t gct ct g t ct Leu Leu A a Leu Ser 595 aaa t ca cgg cct ct g Lys Ser Ar g Pr o Leu 610 cct cgt gt gggac ggt Ar g act Thr gca A a g ac As p aac As n 580 g at As p ct g Le u Ser ggt a31y gct A a ggc a31y 565 aat As n ccg Pr o aat As n 1 699 1 747 1 795 1 843 1891 1 939 1 983 Asp SerI 10 03 n A aI Phe As p A a As pI I I e Leu 75 31y As p 90 li1e ThrI 03 u A a Leu Lys Hi s 03 u 1 55 Page 588 Phe Thr Phe Asn Leu Pr o A a Ph e ValI Hi s ValI A a 140 ValI ValI ValI a31y ValI As p As p Ar g 125 Thr Le u ValI li1e I Ie Cys Le u a31y 110 Tyr A a Pr o ValI Le u Cys Le u A a Ser Hi s Thr O31n Pr o Ser A a a31y Pr o a31y Se r Se r Tr p 1 seqi i st car r ect ed. t xt Oy Val On Phe His Pro Olu Ser lie Oly Oy On Phe 165 170 G3 y Hi s G3 n 1 I Ie Thr Thr O31y 225 Thr Le u ValI A a A a 305 O31n A a Ser Thr Thr 385 As n Le u ValI O31n Ar g 465 Ar g O31u Le u Ly s Ly s 1 95 Ph e Ser As n A a Tyr 275 Thr A a O31u Ar g As p 355 O31y As p Thr Ser Ser 435 As p O31u A a Tyr Pr o 515 Ph e I Ie Hi s Le u O31y 245 Ser O31y Ser O31u Ph e 325 Pr o Tyr Ser A a Tyr 405 Pr o Pr o O31u Le u Pr a 485 Thr Ser As n Le u Ser 215 As p O31y A a O31u Pr a 295 As p O31u Ser As p O31u 375 Le u A a Ar g Lys5 I Ie 455 li1e Thr Hi s I Ie A a 1 85 ValI A a Ser Ph e O31y 265 Lys5 A a ValI I Ie Hi s 345 li1e cys A a Le u I Ie 425 Thr O31u As p Lys5 Le u 505 Cys Thr Ser Tr p Pr a 235 Thr O31y O31u Le u Le u 315 Lys5 A a Ar g Thr Le u 395 Le u I Ie Pr a Ar g ValI 475 Ser Ser Tyr A a Le u 220 Le u Tr p Ph e A a I Ie 300 Le u Le u Le u A a Thr 380 A a O31y As p Ar g Ser 460 Ar g Ly s Thr Ar g A a 205 As p A a Le u Ar g O31y 285 Ph e A a Hi s O31n O31n 365 Ly s Le u As p Ser O31y 445 As n As n Le u ValI Tr p 1 ValI As p Ar g Ly s Le u 270 A a A a Le u As n ValI 350 O31u Le u Ar g Thr A a 430 Ar g Pr a As p Ph e Ser 510 O31n Ph e A a Thr O31u 255 O31y Ar g As p O31y Le u 335 Ar g Le u O31n O31y Ser 415 O31y Thr Ly s Le u As p 495 A a Le u O31u O31n Ly s 240 As p Tr p A a Ar g O31u 320 ValI As p I Ie O31y A a 400 li1e Tyr A a As p A a 480 ValI O31u Val Arg Ala Ala Phe Pro cOy 525 Page 589 seqI i st car r ec 0Gy Ser I'bt Thr G31y Ala Pro Lys Leu Ar g Thr 530 535 G31u Leu G31u Ala Ala Pr o Ar g G31y I Ie Ty r Ser 545 550 555 Phe Ser Leu Asp G3 y A a Val Asp Leu Ser I'b t 565 570 Val Ilie G31n Asn Asn Hi s Val G31u Tyr G31y Val 580 585 Ala Leu Ser Asp Pr o G3 u Ala G3 u Tr p G3 u G3 u 595 600 Ar g Pr o Leu Leu Asn Leu Phe a31y Val a31u Phe 610 615 <210> 461 <211> 747 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221> CIDS <222> (724) <223> RXA00958 <400> 461 at tct aat cc t caat ct gaa gccgat gaga cgt tgcacaa ccat t gcgct t gct gct ggt t ccact t t gg aggt cat ccg ted. t xt I'bt au Ilie IlieAsp 540 a3 y a3 y Leu a3 y Ty r 560 Val I Ie Ar g Thr Leu 575 a3 y a3 y A a Leu Leu 590 li1e Ar g Val Lys Ser 605 Pr o 620 ggcgt at gcc gt gt t gaat g at g aca cac gt t gt t I'bt Thr Hi s Val Val ct c Le u gcc A a g aa a31u cct Pr o ac a Thr ct c Le u ggc a31y gt t ValI g at As p gcc A a at t I Ie t ac Tyr a31y g aa a31u ac c Thr gca A a 120 c ac Hi s t at Tyr gca A a gcc A a at t I Ie c ac Hi s aac As n ct t Le u t ct Ser t gc cys aac As n gcg A a tt a Le u ggc a31y at c I Ie act Thr gt c ValI gt g ValI g ac As p aac As n ggt a31y gt t ValI act Thr 110 gt t ValI aac As n cgc Ar g at c I Ie at g vb t t gc cys cct Pr o gca A a cct Pr o gt g ValI ac g Thr ct t Le u ct g Le u ggc a31y a31y gt g ValI gcg A a cca Pr a cct Pr a gag a31u c ag a31n gt g ValI 1 00 ag c Se r cat cca gaa at c His Proc3u Ilie 130 Page 590 cca ggc cgc aag gtt cca att ggc c Pro G31y Ar g Lys Val Pr o Ilie 31y P 135 140 gt t gcc cca gac ggt at t gaa tca c Val Al a Pr o Asp 31y Ilie G31u Ser L 150 155 ggt gat gt c at c at g gcg gca cgc a G31y Asp Val Ilie I'b t Al a Al a Ar g T 1 70 ct g cag t tt cac cct gag tca gt g c Leu O31n Phe Hi s Pr o G31u Ser Val L 185 1 t tg t cc cgc t gt gt c gaa cag ct t c Leu Ser Ar g Cys Val G3 u O31n Leu L 200 205 t ca <210> 462 <211> 208 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn lIi st cor r ect ed. t xt gt t at cac t ca ct g ggc t gc gt g ~rg Tyr Hi s Ser Leu G31y Cys Val 145 t a ggt acc t gt t cc t cg gag at t .eu 03 y Thr Cys Ser Ser 03 u I I e 160 165 cc acc gat gga aag gcc att ggc hr Thr Asp 03 y Lys Al a I I e 03 y 175 180 t a agc cca acg ggt cct gt c at t .eu Ser Pr o Thr G31y Pr o Val I Ie 90 195 t c gcg aac t aat aaaaaa aggatttgat .eu Al a Asn <400> 462 I'bt Thr H Leu Val A As n Thr V Cys Leu S Al a Leu 1 Leu 03 y T Cys (1y P 31y Val C3 Asp Hi s P 130 Ser Leu C3 Cys Ser S (3 y Lys A Thr 03 y P is Val Val sp Al a Phe a1 Pr o Val er Pr o G31y Ie G31u Ar g yr 03 n Al a ro Val Hi s 1 00 In Ser Pr o 15 r o G31u I Ie y Cys Val er 03 u I I e 1 65 I a I Ie G31y 1 80 r o Val I Ie Leu I I e Al a Val 03 u Thr Pro G31y 55 Thr Leu 70 Leu I I e 03 y Thr Val Phe Pro G31y 135 Val Al a 150 31y As p Leu 03 n Leu Ser As p Al a I Ie 40 Tyr G31y 031u Thr Al a 120 Ar g Pr o ValI Ph e Ar g 200 As n G31y Le u Pr o O31n Tyr As p 1 G31y Lys5 As p I Ie Hi s 1 Cys Hi s 10 Tyr Al a Al a I Ie Hi s 90 As n Le u ValI G31y vb t 1 70 Pr o As p Lys5 Al a As p Pr o G31y vb t Al a Pr o I Ie 1 Al a G31u Ser Cys As n Al a Le u G31y I Ie Thr I Ie 140 G31u Al a Ser Ph e Thr Pr o G31y Le u Lys5 Le u As p 125 G31y Ser Ar g ValI Tyr Ph e Le u vb t I Ie G31u As p G31u Tyr G31y Thr 1 Ser Val (31u (31n Leu Leu Al a Asn 205 Page 591 seql i st cor r ect ed. t xt <210> 463 <211> 469 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(469) <223> RXN03007 <400> 463 cagtttcacc ctgagtcagt gctaagccca acgggtcctg gaacagcttc tcgcgaacta ataaaaaaag gatttgattc aca Thr gag Gu gac Asp cag G n cgt Ar g ggt Gy at c SIle gt g Val aaa Lys gca Al a cac Hi s gct Al a ttc Phe gac Asp gca Al a t cc Ser 120 ct c Leu gag Gu gca Al a at t l Ie at t SIle gcc Al a ggt Gy t cc Ser aac Asn gt g Val gcg Al a gcc Al a act Thr 75 aac Asn gga Gy ggc Gy gcc Al a ttc Phe ct g Leu ggc Gy ggc Gy acc Thr gt g Val t ac Tyr acc Thr ctt Leu 45 gct Al a gca Al a at c SIle aag Lys ttg Leu ccg Pr o gcc Al a gcc Al a ggt Gy aac Asn ct g Leu 110 aac Asn acc Thr at c l Ie gcg Al a ct a Leu 80 acc Thr aag Lys tcattttgtc ccgctgtgtc atg act tct cca gca 11! Met Thr Ser Pro Ala 1 ccc act cca acc ctg 16 Pro Thr Pro Thr Leu gtg ggt gaa tac gat 21 Val Gy G u Tyr Asp cgt act cgc ggt gag Arg Thr Arg Gy G u ttc ctc gcg gcg gct Phe Leu Ala Ala Ala gat t cc gct ggt act Asp Ser Al a y Thr acc ggc gca tcc ctg Thr y Ala Ser Leu 100 cac ggc aac cgt tcg His G y Asn Arg Ser 115 <210> 464 <211> 123 <212> PRT <213> Corynebacter i um gl ut am cum <400> 464 IVet Thr Ser Pro Ala Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn 1 5 10 Pro Thr Pro Thr Leu Gu u Ala lie G u Val Phe Thr Pro Leu Thr 25 Val Gy u Tyr Asp Asp Val His Ile Ala Ala Leu Leu Ala Thr lie Page 592 Ar g Th r Ar g G31y 31u O31n Ph e 55 Phe Leu Al a Al a Al a Ar g Pr o 70 As p Ser Al a G3 y Th r G3 y G3 y Thr O31y Al a Ser Leu I Ie Al a 1 00 His G31y As n Ar g Ser Val Ser 115 seqI i st car r ect ed. t xt 40 Al aAsp Il e Al a 0 y Al aAl a Lys Al a Phe Pro I Ie Thr G31y Al a G31y Leu Leu 75 As p G31y Al a As n Thr I Ie Asn I Ie Thr 90 Al a Se r G31y G31y Val Lys Leu Val Lys 105 110 Ser Lys Ser 03 y 120 <210O> 465 <21 1> 564 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (541) <223> RXN02918 <400> 465 agat tgt cgc tt t tcccat t t ct ccgggt t t tct ggaact t gat tct at t at tgccaaat cagaaagcag gagagacccg tt t tgggcgt at gct gggaa at g agc gaa at I'bt Ser (31u I I c ct a e Leu g aa (31u ct c Le u ac c Thr g ac As p ct a Le u ggt G31y aag cca Pr o t at Tyr t ca Ser tt c Ph e gag (31u gt g ValI ct t Le u aac As n g ac As p 120 gca Al a ct g Le u g at As p g ac As p at g vb t gga G31y c aa O31n gca Al a ccc Pr a gca Al a t t a Le u ggc G31y gct Al a 75 gct Al a at c I Ie gca Al a c ac Hi s c aa O31n ggt G31y t ac Tyr g aa (31u g at As p aac As n gca Al a tt t Ph e gct Al a tt a Le u 45 ggc G31y aac As n aaa Lys5 ac c Thr g at As p 125 gga G31y t cc Ser g ac As p g ac As p aaa Lys5 t cg Ser gca Al a 110 cgc Ar g aaa Lys5 15 ggc G31y gga G31y gca Al a gca Al a at c I Ie 95 tt g Le u tt c Ph e ac c Thr g at As p ct c Le u tt c Ph e ac a Thr 80 cgg Ar g aag Lys5 g at As p 1 g aa (31u ccc Pr a t ct Ser ct g Le u 65 ggt G31y ct g Le u t at Tyr gag (31u g aa (31u at t I Ie gga G31y 50 ct g Le u ggc G31y cat Hi s tt t Ph e g at As p 130 gcc Al a gag (31u 35 aac As n gt t ValI gt g ValI gt t ValI gcg Al a 115 g ac As p ac a Thr 20 gt t ValI t ac Tyr gca Al a aat As n g aa (31u 1 00 ct c Le u ct g Le u gca Al a c ac Hi s ac c Thr gcg Al a ct g Le u t cc Se r t cc Se r t ct Se r gag ctt gcc aac ttg agt gaa gag ctg cgc gga cag ctg gac Page 593 seql i st cor r ect ed. t xt G u Leu Ala Asn Leu Ser G u G u Leu Arg y n Leu Asp 135 140 145 taattgtctc ccatttaagg agt <210> 466 <211> 147 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 466 Met Ser G u I e Leu G u Thr Tyr Trp Ala Pro His Phe G y 1 5 10 G u G u Ala Thr Ala Leu Val Ser Tyr Leu Ala G n Ala Ser 25 Pro Ile G u Val His Thr Leu Phe G y Asp Leu G y Leu Asp 40 Ser G y Asn Tyr Thr Asp Thr G u I e Asp G y Tyr G y Asp 55 Leu Leu Val Ala Ala Leu Ser Val Leu IVet Ala G u Asn Lys 70 G y y Val Asn Leu G y u Leu G y y Ala Asp Lys Ser 90 Leu His Val u Ser Lys G u Asn Thr n Ile Asn Thr Ala 100 105 110 Tyr Phe Ala Leu Ser Pro G u Asp His Ala Ala Ala Asp Arg 115 120 125 G u Asp Asp Leu Ser G u Leu Ala Asn Leu Ser G u G u Leu 130 135 140 G n Leu Asp 145 <210> 467 <211> 735 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(712) <223> RXN01116 <400> 467 tgccaggaat ttacgtcaac cgcgtcgtcc acgttggacc gcaggaaacc acaggacggt gtctaactaa tgacttggga tcataaccaa atg gca gcc Lys Gy Gy Al a Al a SIe Leu Phe Ar g ggaatcgaaa cgc gtt gcc cag gaa ctt gaa gac ggc cag tac gtc aac Ala n u Leu u Asp Gy n Tyr Val Asn 15 cct aca ctt atc ccc ggc tac ctg cct gag gga Pro Thr Leu lie Pro G y Tyr Leu Pro G u Gy 30 Page 594 Vet Ala Ala Arg Val 1 ctc ggc atc ggc atg Leu y lie y IVet cta gag gtt atc ctt Leu G u Val lie Leu cac tcc gaa aac ggt gtg ctg gg His Ser G u Asn G y Val Leu G 4 seql i st cor r ect ed. t xt c gtt gga cct tac cc y Val y Pro Tyr Pr a act gaa gag o Thr G u G u gaa ctt gat cct gag ctg atc aac gcc Su Leu Asp Pro u Leu Ile Asn Ala 60 gca cct ggc gca tcc tac ttc tcc tct Ala Pro y Ala Ser Tyr Phe Ser Ser 75 cgc tcc aag tct gtc gac gtt gca gtc Arg Ser Lys Ser Val Asp Val Ala Val cag tac ggc gac ctg gcc aac tgg atg G n Tyr y Asp Leu Al a Asn Trp Ibet 105 110 ggt atg ggt ggc gca atg gat ctg gtg Gy IVet Gy y Ala IVet Asp Leu Val 120 125 gcc atg acc gat cac atc acc aag aag Ala IVet Thr Asp His Ile Thr Lys Lys 135 140 gag tgt cgc ctc cca ctg act ggc gcg G u Cys Arg Leu Pro Leu Thr y Al a 150 155 acc acc cac gct gtg ttc tct gtg gac Thr Thr His Ala Val Phe Ser Val Asp 170 atc gag tgc gcc gac ggt gtc acc gtt Ile u Cys Ala Asp y Val Thr Val 185 190 gaa gcc gat ttc aaa gtt gct taagcaal G u Ala Asp Phe Lys Val Ala 200 <210> 468 <211> 204 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 468 bet Ala Ala Arg Val Ala n u Leu 1 5 Leu Gy lie Gy IVet Pro Thr Leu Ile 25 Leu G u Val lie Leu His Ser G u Asn 40 Tyr Pro Thr G u G u G u Leu Asp Pro G u Thr lie Thr Val Al a Pro y Al a 70 Ser Phe Ala Iet Ile Arg Ser Lys Ser aag Lys gat Asp ggc Gy ccc Pr o ggc Gy gct Al a tgc Cys 160 gaa G u gaa G u gaa G u tct Ser gtt Val ggc Gy gca Al a ccg Pr o 145 gt g Val gaa G u ct c Leu acc at c Thr Ile ttc gcc Phe Al a atg gaa Vbet u aag ct g Lys Leu 115 t cc aag Ser Lys 130 aag at c Lys II e gac at g Asp IVet ggc ctc G y Leu cgc gaa Arg G u 195 acg Thr at g Met gtc Val 100 gtc Val at c SIe ctt Leu att SIe acg Thr 180 at c SIe acg ctgcgcaatt aag Su Asp 10 Pro Gy Sy Val I G u Leu Ser Tyr I Val Asp 90 Page 595 G n Leu Gy Asn Ser Al a Tyr Pr o Val Al a Ser Val Val G u Gy Gy Ser Leu ValI G31y Al a Pr o 145 ValI (31u Le u (31u Le u 115 Ly s I Ie vb t Le u (31u 1 95 Ser Ly s I Ie Ly s ValI 1 65 Le u Thr O31n G31y Al a (31u 150 Thr SI e (31u Tyr vb t vb t 135 cys Thr (31u Al a seql i st car r ect ed. t xt G31y Asp Leu Al a Asn Tr p 1 05 03 y 03 y Al a I'b t As p Leu 120 125 Thr As p Hi s I Ie Thr Lys 140 Ar g Leu Pr o Leu Thr G31y 1 Hi s Al a Val Phe Ser Val 1 70 Cys Al a As p G31y Val Thr 1 85 As p Phe Lys Val Al a 200 vb t 110 ValI Lys5 Al a As p ValI 1 I Ie Hi s G31y Pr o 1 (31u <210O> 469 <21 1> 876 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (853) <223> RXN01115 <400> 469 ct caccgcat gcagcgt gaa aacact cagt acggact ggc gccagggt ct t gcagct gt c t t t gaaaagg agaact aaaa ag c Ser tt g Le u aag g aa (31u ct g Le u ct a Le u cgt Ar g t cc Ser t cc Ser tt c Ph e at c I Ie ac c Thr ct c Le u ac c Thr gga G31y ac c Thr gt c ValI G31y g at As p gga G31y gca Al a act Thr gac As p at c SI e acc Thr gac As p 75 gcg Al a gca Al a t cc Ser at g vb t gct Al a ccc Pr o ct g Le u gt t ValI tt c Ph e g ac As p t gg Tr p gt t ValI 45 act Thr ggt G31y gca Al a at g vb t aac As n ct g Le u 30 g at As p gt g ValI gt c ValI c aa O31n t gt Cys 110 ac c Thr 15 cca Pr o c ac Hi s gcg Al a gga G31y t ac Tyr 95 ac c Thr tt g Le u c ag O31n cgc Ar g g at As p aac As n 80 tt g Le u gct Al a caccat gt gc at cggt ggcg at g gct at t t tg cac 11! I'b t Al a I I e Leu Hi s 1 gt g t tc at t ggc t cg 1& Val Phe I Ie G31y Ser ct g gat gcc t tg cat 21 Leu Asp Al a Leu gga cat ggt ct g t ct O3 y Hi s 03 y Leu Ser ct g gcg cag gat gt g Leu Al a 03 n Asp Val t tc ggc gt c at c gga Phe G31y Val Ilie G31y gcg gcc acc t ct gat Al a Al a Thr Ser Asp 1 00 gca aaa ttc ggc gag Al a Lys Phe 03 y 03 u 115 Page 596 s ccc cag ggc tgg cta gat cgc gcc Pro n y Trp Leu Asp Arg Al a 120 125 eql i st cor r ect ed. t xt gca gcg tgc cgc ga Ala Ala Cys Arg G a aac ggc act u Asn G y Thr ggt Gy ttg Leu 150 ggc Gy tgg Tr p at c SIle at t l Ie gcc Al a 230 ct a Leu ct g Leu aac Asn cca Pr o ttc Phe ggt Gy 200 gat Asp cac Hi s cag G n gaa G u cca Pr o gag G u 170 gat Asp gat Asp gtt Val cca Pr o gct Al a gcg Al a 155 ggt Gy cgc Ar g gac Asp ggc Gy acc Thr 235 gt g Val 140 t cc Ser t ac Tyr ct g Leu ccc Pr o ggc Gy 220 gt g Val at c SIle cgc Ar g gcg Al a gga Gy t cc Ser 205 gag Gu gaa G u cag G n gag G u ct g Leu gaa G u 190 act Thr t cc Ser cgt tgg Tr p ttc Phe 160 tgc Cys acc Thr cca Pr o gca Al a aac ttc Phe 145 gaa G u gag G u gt g Val gca Al a gag Gu 225 gag G u 130 t cc ccc Ser Pro gcc atg Al a Vet gcg ttg Al a Leu cca gt g Pro Val 195 acc gtg Thr Val 210 gt c ct a Val Leu gt a aat Val Asn act Thr gtt Val gca Al a 180 ct c Leu cag G n agc Ser gaa G u tgg Tr p gcc Al a 165 acc Thr acc Thr at c SIe cca Pr o ctg Leu Arg Pro Asn cat ttc gct Hi s Phe Al a 250 t aat gt t gt a ggcat gt t ca caa <210> 470 <211> 251 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 470 Met Ala I e Leu His Ser Val Ser Tyr 1 5 Val Phe lie y Ser Leu y Ser Thr 25 Leu Asp Ala Leu His Lys Asp Phe Arg 40 G y Hi s G y Leu Ser G u Leu I e G u 55 Leu Ala G n Asp Val Leu Asp Thr Leu 70 Phe y Val Ile y Leu Ser Leu y Ala Ala Thr Ser Asp Arg Val Thr Lys 100 105 Ala Lys Phe G y G u Pro n G y Tr p 115 120 Arg G u Asn y Thr G y Ser Leu Ser G y Thr Ser 10 Thr Asp IVet Val I e Al a Sy Thr Pro Asp Asp Leu G y Al a Val 90 Al a Al a Phe Leu Asp Arg G u Al a Val Page 597 Asp Tr p Val Thr Gy Al a Met Al a 125 I le Asn Leu Asp Val Val G n Cys 110 Al a G n Thr Pr o Hi s Al a Sy Tyr Thr Al a Ar g Ph e 145 G31u (31u ValI A a (31u 225 (31u Pr o Le u ValI 1 95 ValI Le u As n Tr p A a 1 65 Thr Thr I Ie Pr o Le u 245 Le u 150 G31y Tr p SI e SI e A a 230 Le u 135 (31u Thr As p A a A a 215 A a A a seql i st car r ect ed. t xt 140 As n As n Pro Ala Se r Ar g 1 55 Pr o Ser 31u G31y Ty r Ala 1 70 Phe Thr As p Arg Leu G31y 1 G31y Ala As p As p Pr o Ser 200 205 As p 1y Val (31y (1y (1u 220 Hi s Val Pr o Thr Val (31u 235 03 n Hi s Phe A a 250 Hi s cys 1 li1e Pr o Ar g Pr o Ph e 1 cys Thr Pr a A a As n 240 <210O> 471 <21 1> 1284 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (1261) <223> RXS00116 <400> 471 cgcggcacgc acgct ggggg caagcgt cga caagcacaaa t t tgcgcacc aat caat ggg ggat caaat a t agt agct gc ct tt t tgct t aat tgaat cc at g agt aat gac t tc 11! l'bt Ser Asn Asp Phe gt c ValI ac c Thr cct Pr a at t I Ie tt g Le u t ac Tyr at t I Ie t ct Ser cga Ar g gag (31u ggg G31y gca A a cct Pr a gcg A a ct t Le u gt t ValI ggt G31y aat As n gt g ValI t cg Ser gt g ValI ag a Ar g gag (31u cct Pr a aat As n gct A a 75 gag (31u t t g Le u ccc Pr a gcg A a cgt Ar g c ag O31n cgt Ar g gt g ValI ggt (31y ggt G31y gca A a at g vb t t cg Ser cat Hi s at c I Ie gt g ValI 110 g aa G31u 15 at c I Ie tt a Le u gcg A a tt g Le u ac g Thr 95 gag (31u ac g Thr aat As n gag G31u ggg G31y gag G31u gt g ValI cct Pr a tt t Ph e ggt G31y gcg A a ggg G31y Ph e gcc A a g at As p gca A a c ag O31n t cg Ser g at As p g at As p act Thr g aa (31u 115 ac c Thr ggc G31y gag O31u gct A a ct g Le u gag (31u 1 00 gt g ValI Page 598 seql i st cor r ect ed. t xt gtt Val ggg G y gat Asp 150 at g Met aag Lys ttg Leu aag Lys acg Thr 230 ggg Gy aag Lys gcg Al a ggg G y ggg G y 310 at t SIle gtt Val aag Lys ct c Leu gaa G u 120 acg Thr gat Asp at c SIle gcg Al a ttg Leu 200 gt g Val tcg Ser gcg Al a ttt Phe gcg Al a 280 gag G u aag Lys gat Asp gt g Val tcg Ser 360 gaa Gu t at Tyr gt g Val gat Asp aat Asn 170 aag Lys gat Asp gt c Val gcg Al a gca Al a 250 tct Ser gaa G u aag Lys cat Hi s gat Asp 330 gcg Al a aag Lys gcg Al a tac Tyr gcg Al a aag Lys 155 tcg Ser cag G n gag G u gcg Al a gcg Al a 235 ccg Pr o t at Tyr cat Hi s cgg Ar g gac Asp 315 ggt G y at t l Ie gtt Val gag G u gat Asp gtt Val 140 ttg Leu ccg Pr o ttg Leu gt g Val aag Lys 220 aaa Lys gag G u gt g Val gag G u gat Asp 300 agt Ser gcg Al a ccg Pr o cgt Ar g cgt Ar g 380 gcg Al a 125 cct Pr o cat Hi s cat Hi s gcg Al a t at Tyr 205 ct g Leu acg Thr ccg Pr o ggg G y cag G n 285 at t SIle at g Met gag G u gt g Val ttt Phe 365 ct c Leu tat gcg gcg gc Tyr Ala Ala Al ttg Leu gcg Al a aat Asn ggt G y 190 gag G u ccc Pr o ttc Phe ttg Leu gct Al a 270 aag Lys ttg Leu G y ttc Phe cag G n 350 gcg Al a aag Lys cag G n gcg Al a ccg Pr o 175 gtt Val cat Hi s ggt G y aat Asn ttg Leu 255 aca Thr tgg Tr p cgt Ar g acg Thr tgt Cys 335 gcg Al a ttt Phe gag G u gt g Val 160 acg Thr gct Al a ctt Leu at g Met gt g Val 240 gag G u cct Pr o gt g Val act Thr t at Tyr 320 ttt Phe ttt Phe tgc Cys gt Va 14 ac Th gg G cg Ar gt Va tg Tr 22 ac Th gc Al tt Ph tc Se gc Al 30 tt Ph ga gt Va aa Ly t att gcg a I e Al a 130 g gag aac l G u Asn t aag aag r Lys Lys t tcg gtg y Ser Val t gcg tat g Al a Tyr 195 t ttt gat I Phe Asp 210 g gat cgc p Asp Arg t ggt tgg r y Trp g gtg ttg a Val Leu t cag ccg e n Pro 275 a aag at g r Lys IVet 290 g tta gat a Leu Asp c atc gtt e Ile Val g ttg att u Leu Ile g gat cat I Asp Hi s 355 a aaa gaa s Lys G u 370 ttg Leu tcg Ser acg Thr ttt Phe 180 gac Asp gat Asp acg Thr aag Lys aag Lys 260 gct Al a tct Ser aag Lys gcg Al a gag G u 340 ccg Pr o gag G u gcg Al a tgg Tr p cgg Ar g 165 tct Ser ttg Leu cag G n gt g Val acg Thr 245 gcg Al a gt g Val aag Lys gcg Al a gat Asp 325 aag Lys aag Lys acg Thr 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1261 ggg att aag aaa cta Sy Ile Lys Lys Leu 385 Page 599 seql i st cor r ect ed. t xt tagtttgaac aggttgttgg ggg <210> 472 <211> 387 <212> PRT <213> Corynebacter i um gl ut ai cum 1284 <400> 472 IVet Ser Asn Asp Phe Val 1 I e Leu SIle Ar g Ar g Gy Gy Al a Val 145 Thr Gy Ar g Val Tr p 225 Thr Al a Phe Ser Al a G n Ser Asp Asp Thr G u 115 Al a Asn Lys Val Tyr 195 Asp Ar g Tr p Leu Pr o 275 Mbt 5 Mt Phe G n Ser G u Al a SIle Al a Tr p Ar g 165 Ser Leu G n Val Thr 245 Al a Val Lys Thr Pr o SIle Leu 70 Ty r l Ie Val Gy Asp 150 bet Lys Leu Lys Thr 230 Sy Lys Al a Sy Val Ser Arg G n Arg Ala Asp G u Asp 40 Leu G y G y Ar g Al a Al a Asn Pro Asp Thr Al a Thr 105 Leu G u Pro 120 Al a Thr Ar g 135 Val Asp Val Ile Ile Val Lys Ala Leu 185 Val Leu Ser 200 Hi s Val Ser 215 Val Ser Ser Trp Ala Leu G n Phe Met 265 His Ala lle 280 Leu G u Leu 295 Leu 10 Val Sy Asn Val Ser 90 Val Tyr Val Asp Asn 170 Lys Asp Val Al a Al a 250 Ser G u Lys Ar g G u Pr o Asn Al a G u Leu Tyr Al a Lys 155 Ser G n G u Al a Al a 235 Pr o Tyr Hi s Ar g Pr o Al a Ar g G n Ar g Val Sy Asp Val 140 Leu Pr o Leu Val Lys 220 Lys G u Val G u Asp 300 Phe Sy Ar g Tyr Asp Leu Leu Al a 125 Pr o Hi s Hi s Al a Tyr 205 Leu Thr Pr o Sy G n 285 I le Sy Al a Met Ser Hi s SIle Val 110 Tyr Leu Al a Asn Sy 190 G u Pr o Phe Leu Al a 270 Lys Leu G u l e Leu Al a Leu Thr G u Al a G n Al a Pr o 175 Val Hi s Sy Asn Leu 255 Thr Tr p Ar g Ala Leu Asp Lys Ala G y Leu Lys Thr His Asp Ser IVet G y Thr Tyr Page 600 seqI i st car r ect ed. t xt 305 310 315 320 Phe IlIe Val Ala As p IIe Oy AspArg Asp Oy Ala(3u Phe Cys Phe 325 330 335 G31u Leu Ile (1u Lys Val 31y Val Ala Ala Ilie Pr o Val 31n Ala Phe 340 345 350 Val Asp Hi s Pr o Lys Lys Tr p Ser Ser Lys Val Ar g Phe Ala Phe Cys 355 360 365 Lys Lys O31u O31u Thr Leu Ar g O31u Ala Ala O31u Ar g Leu Lys O31y Ilie 370 375 380 Lys Lys Leu 385 <210> 473 <211> 607 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (607) <223> FRXA00116 <400> 473 t t t gcgcacc aat caat ggg ggat caaat a t agt agct gc at gagt aat g act t cgt cgt t tct aggct t agaccct t tg gt gaaacgat tt t tgcaacc at g acc cag cga I'bt Thr O31n Ar g gt t ValI ggt G31y aat As n gt g ValI t cg Ser gt g ValI t at Tyr gt g ValI gcg A a cgt Ar g c ag O31n cgt Ar g gt g ValI ggt G31y g at As p gt t ValI 120 gca A a at g vb t t cg Ser cat Hi s at c I Ie gt g ValI t at Tyr tt g Le u at c I Ie t t a Le u gcg A a t t g Le u ac g Thr 75 gag O31u gcg A a cag O31n aat As n gag (31u ggg G31y gag (31u gt g ValI cct Pr o gcg A a gag O31u ct t Le u at c I Ie cgt Ar g 45 ag g Ar g ggg G31y ggg G31y gct A a gt g ValI 125 ggt G31y gcg A a ggg Ph e gcc A a g at As p at t I Ie 110 gag O31u ggc G31y gag O31u gct A a ct g Le u gag O31u gt g ValI tt g Le u t cg Ser 1 tt t Ph e c ag O31n t cg Ser gag O31u gcg A a at c I Ie gcg A a t gg Tr p cct Pr o at t I Ie tt g Le u t ac Tyr at t I Ie gt t ValI ggg O31y g at As p 130 g at As p ct c Le u ag g Ar g aac As n ac g Thr tt g Le u gcg A a 115 gt g ValI gag O31u ggg O31y gca A a cct Pr o gcg A a g aa O31u 1 00 ac g Th r g at As p gat aag t tg cat gcg gcg gt g act aag aag acg cgg at g at t at c gt t Page 601 seqI i st car r ect ed. t xt As p Lys Leu Hi s Al a Al a Val Thr Lys Lys Thr Arg I'bt Ilie lieVal 135 140 145 aat t cg ccg cat aat ccg acg ggt t cg gtg t tt t ct aag aag gcg tt g As n Ser Pr o Hi s Asn Pr o Thr O31y Ser Val Phe Ser Lys Lys Al a Leu 150 155 160 165 aag cag ttg gcg Lys 03 n Leu Al a <210O> 474 <211> 169 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 474 I'bt Thr On Arg AlaVal Ou Ala(3y Ala HeAsn LeuOy On Oy Ph e O31n Ser O31u Al a I Ie Al a Tr p Ar g 145 Ser As p Le u Ar g As n Thr Le u Al a 115 ValI I Ie Lys5 As p G31y Al a As p Thr Pr o Ar g ValI ValI Le u 1 Pr o As n Al a O31u Le u Tyr Al a Lys5 135 Ser O31n Ar g Tyr As p Le u Le u Al a 1 05 Pr o Hi s Hi s Al a 10 vb t Ser Hi s I Ie ValI 90 Tyr Le u Al a As n Le u Al a Le u Thr 75 O31u Al a O31n Al a Pr o 1 55 O31u G31y O31u ValI Pr o Al a O31u ValI 140 Thr I Ie Ar g Ar g G31y G31y Al a ValI 125 Thr G31y Al a G31y Ph e Al a As p I Ie 110 O31u Lys5 Ser Ser As p As p Thr O31u Al a As n Lys5 ValI O31u Al a Le u O31u ValI Le u Ser Thr Ph e 1 <210O> 475 <21 1> 843 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CIDS <222> (820) <223> RXS00391 <400> 475 at ct t gt aga t cccaccgca at t ct gggag at ct cgagga cgcaat ct ct ggaagaaaac t t tcct cccc at ccct gt ac aagat aaaac ccgt gcacag t tg ct g cgc gat t ct 115 Leu Leu Arg Asp Ser 1 Page 602 seql i st cor r ect ed. t xt caa cga gtt ggc G n Arg Val y act Thr aat Asn ggc G y gt g Val ct c Leu ct g Leu tta Leu ttt Phe at t SIle 150 t ca Ser gcg Al a at t SIle gag G u ggt Sy gt g Val tgg Tr p ctt Leu aca Thr aat Asn aaa Lys 120 gt c Val gcg Al a act Thr gt c Val cag G n 200 tgg Tr p ct c Leu aca Thr t cc Ser ctt Leu agc Ser ttt Phe ggc Gy at g lbt ctt Leu cag G n ggt Gy 170 at t SIle t ac Tyr acc Thr gcc atc gat Al a li e Asp ggt G y gcc Al a gcc Al a ct c Leu 75 cac Hi s gac Asp gac Asp gtt Val ct a Leu 155 ggc Gy tcg Ser aga Ar g acc Thr acc Thr gat Asp at g lbt at t SIle at t SIle cgc Ar g t cc Ser ggc Gy 140 gac Asp tgc Cys gat Asp aat Asn tct Ser 220 cct tcg atc gct ccg Pr o gct Al a 45 cca Pr o gct Al a gac Asp gt c Val ttg Leu 125 ggt Gy at c SIle gtt Val gag G u gca Al a 205 gat Asp Pr o aag Lys acg Thr gca Al a gga G y gct Al a t at Tyr 110 caa G n gct Al a aac Asn t at Tyr cgc Ar g 190 cct Pr o t ca Ser Ser 15 ggc G y cat Hi s cac Hi s gtt Val ttc Phe 95 aca Thr ggc G y gca Al a at t SIle gat Asp 175 at t SIle gaa G u ggt G y I e Al a gct cag Al a G n cag ttt G n Phe cac att His Il e gag cca G u Pro 80 gca ggc Al a G y tcc ttg Ser Leu att gaa Ile G u ttg tct Leu Ser 145 gtc acc Val Thr 160 ggc aag G y Lys gag ttg G u Leu cat ccg His Pr o gaa ct c G u Leu 225 ttg gtg Leu Val ct c act Leu Thr tta ggt Leu G y gca ggc Al a G y ct a gct Leu Al a gcc gcg Al a Al a act cca Thr Pro 115 gcc ctg Al a Leu 130 aag cag Lys G n acc tac Thr Tyr ccc att Pro Ile ggt ggc G y G y 195 gat ttc Asp Phe 210 cac gac His Asp at g Met ccg Pr o ggc G y at g Met at t l Ie gca Al a 100 at g Met aaa Lys gcc Al a ggc G y ccc Pr o 180 ccg Pr o gcc Al a ggg G y gcc Al a ttg Leu gaa G u cag G n gat Asp gaa G u cag G n ctt Leu cga Ar g t cc Ser 165 ggc G y at g Met aac Asn att SIe ctc acc gtg act ggt cgc gtg gat acc cgt Leu Thr Val Thr y Arg Val Asp Thr Arg 230 235 tgc <210> 476 <211> 240 <212> PRT <213> Corynebacter i um gl ut ai cum cat Hi s 240 tgattccggt ggattgaagt 840 Page 603 seql i st cor r ect ed. t xt <400> 476 Leu 1 Al a G n Phe I le Pr o Gy Leu G u Ser 145 Thr Lys Leu Pr o Leu 225 Leu Arg Asp Leu Val Met Leu Thr Pro Leu G y G y Al a G y let Leu Al a Il e Al a Al a Al a 100 Thr Pro Mbet 115 Ala Leu Lys 130 Lys G n Al a Thr Tyr G y Pro Ile Pro 180 G y G y Pr o 195 Asp Phe Al a 210 Hi s Asp G y Ser 5 Al a Leu G u G n Asp G u G n Leu Ar g Ser 165 Gy Met Asn SIle Sn Arg Val y Ala lie Asp Pro Thr Asn Gy Val 70 Leu Leu Leu Phe SIle 150 Ser Al a SIle G u Leu 230 Ser Leu G n Leu Ser Lys Leu Asp 135 Ser G u Lys Al a Gy 215 Thr Ser 25 Ser Leu Ar g Gy Thr 105 Al a SIle G u Ser Ar g 185 Gy Phe Thr Gy Al a Al a Leu 75 Hi s Asp Asp Val Leu 155 Gy Ser Ar g Thr Ar g 235 Pr o Al a Pr o Al a Asp Val Leu 125 Gy I e Val G u Al a 205 Asp Asp Lys Thr Al a Gy Al a Tyr 110 G n Al a Asn Tyr Ar g 190 Pr o Ser Thr Ser Ile G y Al a Hi s G n Hi s Hi s Val G u Phe Al a Thr Ser Sy lle Al a Leu Ile Val 160 Asp y 175 Ile u G u Hi s G y G u Arg His 240 <210> 477 <211> 1017 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(994) <223> RXS00393 <400> 477 tctattcatt tcacaatagc gtttcacact cccccatagc ctgccgaacg tatttcaagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt atg tct cac acg gaa 115 IVet Ser His Thr G u 1 Page 604 seql i st cor r ect ed. t xt ccc cag ccg aat tct gta Pro n Pro Asn Ser Val act ttg tcc Thr Leu Ser ccg Pr o gt c Val ctt Leu t ac Tyr cga Ar g gcg Al a ct g Leu ggc Gy ct c Leu 150 gga Gy gcc Al a aat Asn gcg Al a at t SIle 230 cca Pr o ggg Gy acc Thr gct Al a gt g Val gat Asp act Thr at t l Ie agc Ser 120 tgg Tr p gag G u cag G n gtt Val cgc Ar g 200 cgc Ar g acg Thr ct g Leu at c SIle gca Al a cat Hi s tgg Tr p at t SIle tct Ser ttc Phe tgg Tr p t ac Tyr gct Al a acc Thr 170 gt g Val at t SIle ggc Gy ttc Phe gcg Al a 250 aac Asn aat Asn gat Asp gct Al a cgt Ar g 75 ggg Gy ggt Gy tgg Tr p acc Thr gt g Val 155 caa G n ggg Gy cca Pr o gat Asp at c SIle 235 at c l Ie aac Asn gcg Al a ggt Gy ttg Leu ggc Gy ttg Leu at c SIle ct g Leu ggc Gy 140 ttc Phe acc Thr tcg Ser acc Thr gcg Al a 220 at g Met at c SIle gcc Al a ttc Phe ttt Phe 45 at c SIle acc Thr gct Al a gca Al a at c SIle 125 ggt Gy at c SIle ggt Gy at g Met gat Asp 205 ggt Gy t cc Ser gtt Val acc Thr gcg Al a gt g Val at c SIle gat Asp gag G u ggt Gy 110 ct c Leu aaa Lys ttc Phe t cc Ser tct Ser 190 agc Ser gct Al a at c SIle ttc Phe ggc G0y 270 tgg att caa ggc gca cgc Trp lie n y Ala Arg gtc att gcc ggt tca ggt Val Ile Ala y Ser y tgg aag gcc ttg ctg gcg Trp Lys Ala Leu Leu Ala gtg aat tac gcc aat gat Val Asn Tyr Ala Asn Asp gac cgc acc ggt cct ctg Asp Arg Thr y Pro Leu 80 aag aaa gtg aaa gct gcg Lys Lys Val Lys Ala Ala 100 gcc ggc acc gcg ctg agc Al a G y Thr Al a Leu Ser 115 ggc atc ctg tgt gtg ctg Gy Ile Leu Cys Val Leu 130 cct tat ggt tac cgc ggg Pro Tyr Gy Tyr Arg y 145 ggc ctc gtc gcg gtc atg G y Leu Val Al a Val Met 160 165 agc tgg gcc ggt ttg gcc Ser Trp Ala y Leu Ala 180 ggc gtg aac ttg gcc aac G y Val Asn Leu Al a Asn 195 acc gga aaa att acc ctc Thr y Lys Ile Thr Leu 210 aag ctg ttc ctc gcg ctg Lys Leu Phe Leu Ala Leu 225 ctg gcg ttt gtc gcc tgg Leu Ala Phe Val Ala Trp 240 245 ctg gca ctg aaa gcc gca Leu Ala Leu Lys Ala Ala 260 gat ctc atc ccc gtc atc Asp Leu Ile Pro Val lle 275 Page 605 seqI i st car r ect ed. t xt ggc tca aca ggg cgc gcc atg gcg ttg tgg gcc gtg ctc acg ggc ctg G31y Se r Thr G31y Ar g Al a I'bt Al a Leu Tr p Al a Val Leu Thr G31y Leu 280 285 290 gca t ta gcg t tt agc t aaaacgct t t tcgacgct c ccc Al a Leu Al a Phe Ser 295 <210> 478 <211> 298 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 979 1017 <400> 478 I'bt Ser Hi s li1e O31n 31y I e Al a (3 y Lys Al a Leu Asn Tyr Al a Ar g Thr (31y Lys Val Lys O3 y Thr Al a 115 Il e Leu Cys 130 Tyr 03 y Tyr 145 Leu Val Al a Tr p Al a (31y Val Asn Leu 1 95 O3 y Lys IlIe 210 Leu Phe Leu 225 Al a Phe Val Al a Leu Lys Leu Ilie Pr o 275 Th r 03 u Al a Ar g Ser 03 y Leu Al a As n As p Pro Leu Al a Al a 1 00 Leu Ser Val Leu Ar g G31y Val [vb t 1 65 Leu Al a 1 80 Al a As n Thr Leu Al a Leu Al a Tr p 245 Al a Al a 260 Val I Ie Pro On Pro Asn Pr o ValI Le u Tyr 70 Ar g Al a Le u G31y Le u 150 G31y Al a As n Al a I Ie 230 Pr o G31y (31y Ar g Al a ValI 55 Ser Le u Ph e Le u Al a 135 G31y Thr Al a I Ie ValI 215 Ser Al a Pr o Ser Thr Al a 40 ValI As p Thr I Ie Ser 120 Tr p O31u O31n ValI Ar g 200 Ar g Thr Le u I Ie Thr 280 Tr p 25 Ph e Al a G31y G31y Ser 1 05 Al a Ph e I Ie Ph e G31y 1 85 As p Le u Pr a I Ie Ar g 265 (31y Ser 10 Al a Hi s Tr p I Ie Ser 90 Ph e Tr p Tyr Al a Thr 1 70 ValI I Ie G31y Ph e Al a 250 As n ValI As n As p Al a Ar g G31y G31y Tr p Thr ValI 1 O31n O31y Pr a As p I Ie 235 li1e As n Thr Al a O31y Le u O31y Le u I Ie Le u O31y 140 Ph e Thr Ser Thr Al a 220 vb t I Ie Al a Le u Ph e Ph e li1e Thr Al a Al a I Ie 125 Oy le Oy vb t As p 205 O31y Ser ValI Thr Ser Al a ValI I Ie As p O31u O31y 110 Le u Lys5 Ph e Ser Ser 1 Ser Al a I Ie Ph e O31y 270 As p Pr a Tr p O31y O31u Pr a ValI I Ie As n Ph e ValI 1 Al a Ar g Cys Pr a 255 Arg Ala N/t Ala Leu Trp Ala 285 Page 606 seql i st cor r ect ed. t xt Val Leu Thr G y Leu Al a Leu Al a Phe Ser 290 295 <210> 479 <211> 1005 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(982) <223> FRXA00393 <400> 479 tctattcatt tcacaatagc gtttcacact cccccatagc ctgccgaacg tatttcaagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt ccc Pr o ccg Pr o gt c Val ctt Leu t ac Tyr cga Ar g gcg Al a ct g Leu ggc Gy ct c Leu 150 gga Gy gcc Al a ccg Pr o acc Thr gct Al a gt g Val gat Asp act Thr at t l Ie agc Ser 120 tgg Tr p gag G u cag G n gtt Val tct Ser gca Al a cat Hi s tgg Tr p at t SIle tct Ser ttc Phe tgg Tr p t ac Tyr gct Al a acc Thr 170 gt g Val gt a Val aat Asn gat Asp gct Al a cgt Ar g 75 ggg Gy ggt Gy tgg Tr p acc Thr gt g Val 155 caa G n ggg Gy act Thr gcg Al a ggt Gy ttg Leu ggc Gy ttg Leu at c SIle ct g Leu ggc Gy 140 ttc Phe acc Thr tcg Ser ttg Leu ttc Phe ttt Phe 45 at c SIle acc Thr gct Al a gca Al a at c SIle 125 ggt Gy at c SIle ggt Gy at g let t cc Ser gcg Al a gt g Val at c SIle gat Asp gag G u ggt Gy 110 ct c Leu aaa Lys ttc Phe t cc Ser tct Ser tgg Tr p gt c Val tgg Tr p gt g Val gac Asp 80 aag Lys gcc Al a ggc Gy cct Pr o ggc Gy 160 agc Ser tct cac acg gaa Ser His Thr G u caa ggc gca cgc Sn G y Ala Arg gcc ggt tca ggt Al a y Ser G y gcc ttg ctg gcg Ala Leu Leu Ala tac gcc aat gat Tyr Al a Asn Asp acc ggt cct ctg Thr y Pro Leu gtg aaa gct gcg Val Lys Ala Ala 100 acc gcg ctg agc Thr Ala Leu Ser 115 ctg tgt gtg ctg Leu Cys Val Leu 130 ggt tac cgc ggg Sy Tyr Arg y gtc gcg gtc atg Val Al a Val Met 165 gcc ggt ttg gcc Al a y Leu Al a 180 gct ggc gtg aac ttg gcc aac Ala Gy Val Asn Leu Ala Asn Page 607 aat Asn gcg Al a at t SIle 230 cca Pr o ggg Gy gct Al a cat Hi s seql i st cor r ect ed. t xt 185 190 att cgc gat att cca acc gat agc aag acc gga aaa lie Arg Asp lie Pro Thr Asp Ser Lys Thr y Lys 200 205 210 gtc cgc ctg ggc gat gcg ggt gct cgt aag ctg ttc Val Arg Leu y Asp Ala y Ala Arg Lys Leu Phe 215 220 225 tcc acg ccg ttc atc atg tcc atc tgc ctg gcg ttt Ser Thr Pro Phe Ile Met Ser lie Cys Leu Ala Phe 235 240 gcg ctg atc gcg atc atc gtt ttc ccg ctg gca ctg Ala Leu Ile Ala Ile Ile Val Phe Pro Leu Ala Leu 250 255 ccg atc cgc aac aac gcc acc ggc aag gat ctc atc Pro lie Arg Asn Asn Ala Thr y Lys Asp Leu Ile 265 270 caa cag ggc gcg cca tgg cgt tgt ggg ccg tgc tca G n G n G y Al a Pro Trp Arg Cys G y Pro Cys Ser 280 285 290 tagcgtttag ctaaaacgct ttt 195 at t SIle ct c Leu gt c Val aaa Lys ccg Pr o 275 cgg Ar g acc Thr gcg Al a gcc Al a gcc Al a 260 t ca Ser gcc Al a ct c Leu ctg Leu tgg Tr p 245 gca Al a tcg Ser tgg Tr p 1005 <210> 480 <211> 294 <212> PRT <213> Corynebacteri um gl utami cum <400> 480 IVet Ser Hi s 1 lie Gn y I e Al a G y Lys Ala Leu Asn Tyr Al a Arg Thr G y Lys Val Lys G y Thr Al a 115 Ile Leu Cys 130 Tyr G y Tyr 145 Thr G u 5 Al a Ar g Ser G y Leu Al a Asn Asp Pro Leu Al a Al a 100 Leu Ser Val Leu Arg G y Pro G n Pro Asn Ar g Al a Val 55 Ser Leu Phe Leu Al a 135 Sy Thr Al a 40 Val Asp Thr SIle Ser 120 Tr p G u Tr p 25 Phe Al a Sy Sy Ser 105 Al a Phe SIle Ser 10 Al a Hi s Tr p SIle Ser 90 Phe Tr p Tyr Al a Val Asn Asp Al a Ar g Sy Sy Tr p Thr Val 155 Thr Al a Sy Leu Sy Leu SIle Leu Sy 140 Phe Leu Phe Phe I le Thr Al a Al a SIle 125 Gy lie Ser Al a Val SIle Asp G u Sy 110 Leu Lys Phe Asp Pr o Tr p Sy G u Pr o Val SIe Asn Phe Leu Val Ala Val Met G y Thr G n Phe Thr G n Thr y Ser Val Ser Page 608 seql i st cor r ect ed. t 165 170 Trp Ala y Leu Ala Al a Ala Val y Val y Ser 180 185 Val Asn Leu Ala Asn Asn Ile Arg Asp Ile Pro Thr 195 200 Sy Lys lie Thr Leu Al a Val Arg Leu y Asp Al a 210 215 220 Leu Phe Leu Ala Leu I e Ser Thr Pro Phe Ile Iet 225 230 235 Ala Phe Val Ala Trp Pro Ala Leu Ile Ala lie Ile 245 250 Ala Leu Lys Ala Ala G y Pro lie Arg Asn Asn Al a 260 265 Leu lie Pro Ser Ser Al a n G n y Ala Pro Trp 275 280 Cys Ser Arg Ala Trp His 290 <210> 481 <211> 987 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(964) <223> RXS00446 <400> 481 tgctacgaag ttatctagta atgaagttag tttttcccct ctcc ggtgacggag gctacttggg gggctaatcg gtacccggat at g txt Mt Asp 205 Gy Ser Val Thr Ar g 285 Ser 190 Ser Al a SIle Phe Gy 270 Cys 175 Al a Lys Ar g Cys Pr o 255 Lys Gy ccggcag cagttgatgc ggt gcg gtt gag ct c Leu acg Thr acg Thr gct Al a ccg Pr o at c SIle gag G u ggt Gy gct Al a cca Pr o act Thr gat Asp ctt Leu ggc Gy ggc Gy ttc Phe gat Asp acc Thr gca Al a tcg Ser gat Asp gcg Al a cag G n 75 cgc Ar g gag G u tct Ser gag G u cag G n aat Asn ct c Leu cat Hi s gcg Al a gt c Val 45 gt c Val cat Hi s at t SIle tta Leu ct g Leu at t SIle gcg Al a gat Asp ttc Phe gag gtt G u Val 15 tgt caa Cys n ttt cca Phe Pro ggc gcc G y Al a ctt gat Leu Asp 80 atc tgc I e Cys 95 Page 609 e t y Ala Val Gu 1 gag ttt gac cag gtc G u Phe Asp G n Val cag ctg gtt cag gca G n Leu Val G n Ala tgg cgc agc ttt gag Trp Arg Ser Phe G u act cct gtt gcc att Thr Pro Val Ala lle gcg atg gca gcc gcg Al a Vet Al a Al a Al a aac ccc aac aat cct Asn Pro Asn Asn Pro 100 seql i st cor r ect ed. t xt tcg ggc acc acc atc acc cag gcg cag ttt gat aat ttc atg gaa aag 451 Ser y Thr Thr Ile Thr G n Ala G n Phe Asp Asn Phe Met G u Lys 105 110 115 gtt cca aac gat gtc gtt gtt ggg ctg gat gag gct tat ttt gag ttc 499 Val Pro Asn Asp Val Val Val y Leu Asp G u Al a Tyr Phe u Phe 120 125 130 aac cgc gcg gac gac acc cca gtt gcc act gag gaa atc cac cgc cac 547 Asn Arg Ala Asp Asp Thr Pro Val Ala Thr G u u lie His Arg His 135 140 145 gac aac gtg att ggt ttg cgc acg ttc tcc aag gcg tat ggc ctg gcg 595 Asp Asn Val Ile y Leu Arg Thr Phe Ser Lys Ala Tyr y Leu Ala 150 155 160 165 ggc ttg cgt gtt ggt tac gcc ttc gga aac gca gag atc atc gca gcg 643 Sy Leu Arg Val y Tyr Ala Phe y Asn Ala u lie ile Ala Ala 170 175 180 atg aat aag gtg gct att cct ttc gcg gtg aat tca gca gct cag gcg 691 IVet Asn Lys Val Ala Ile Pro Phe Ala Val Asn Ser Ala Ala G n Ala 185 190 195 gca gcg ctt gcg agt ttg aat tct gcc gat gag ttg atg gaa cgg gtg 739 Ala Ala Leu Ala Ser Leu Asn Ser Ala Asp G u Leu IVet G u Arg Val 200 205 210 gag gaa acc gtc gaa aag cgt gat gct gtg gtg tca gcg ctt ggt gct 787 G u G u Thr Val G u Lys Arg Asp Ala Val Val Ser Ala Leu y Ala 215 220 225 gcg ccg acg cag gcc aat ttc gtc tgg ctg ccg ggc gag ggc gcc gct 835 Ala Pro Thr G n Ala Asn Phe Val Trp Leu Pro Gy G u Gy Ala Ala 230 235 240 245 gag ttg gcg gct aaa ttg gcc gag cac ggc atc gtg att cgc gcg ttc 883 G u Leu Ala Ala Lys Leu Ala G u His G y IIe Val Ile Arg Ala Phe 250 255 260 ccc gag ggt gcg cgc att tcg gtg acc aac gcc gag gaa act gac aag 931 Pro G u y Ala Arg I e Ser Val Thr Asn Ala G u G u Thr Asp Lys 265 270 275 ctg ctg cgc gcg tgg gag gcc atc aat gct ggg tagtctttgg cgttttgcgg 984 Leu Leu Arg Ala Trp u Ala lie Asn Ala y 280 285 tgc 987 <210> 482 <211> 288 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 482 Met G y Ala Val G u Leu Arg G u Ala Leu Ala G u His Leu G u Val 1 5 10 G u Phe Asp G n Val Thr Val y Cys y Ser Ser Ala Leu Cys G n 25 Sn Leu Val n Ala Thr Cys Ala n y Asp u Val lie Phe Pro 40 Trp Arg Ser Phe G u Al a Tyr Pro Ile Phe Ala G n Val Ala Gy Ala 55 Page 610 seqI i st car r ect ed. t xt Thr Pro Val Ala Ilie Pro Leu Thr Al a Asp 03 n Asn Hi s Asp Leu Al a As n As n Al a (31u 145 Al a (31u Ser Le u Ser 225 G31y ValI a u Al a As n vb t 115 Ph e Hi s Gy le Al a 1 95 (31u Le u G31y Ar g Thr 275 Al a Pr o Ly s Ph e Hi s Al a 1 65 Al a Al a ValI Al a Al a 245 Ph e Ly s I Ie Ser ValI As n As p 150 G31y vb t Al a O31u Al a 230 O31u Pr o Le u Thr G31y Pr o Ar g 135 As n Le u As n Al a O31u 215 Pr o Le u O31u Le u As p Thr As n 120 Al a ValI Ar g Ly s Le u 200 Thr Thr Al a G31y Ar g 280 Ly s Thr 1 05 As p As p I Ie ValI ValI 1 85 Al a ValI O31n Al a Al a 265 Al a Ar g Thr ValI Thr Le u 1 55 Tyr I Ie Le u Ly s As n 235 Le u I Ie aOu Le u O31n ValI Pr o 140 Ar g Al a Pr a As n Ar g 220 Ph e Al a Ser Al a I Ie Al a G31y 125 ValI Thr Ph e Ph e Ser 205 As p ValI O31u ValI I Ie 285 Ph e O31n 110 Le u Al a Ph e G31y Al a 1 Al a Al a Tr p Hi s Thr 270 As n lIe Ph e As p Thr Ser As n 1 ValI As p ValI Le u G31y 255 As n Al a As p cys As p (31u (31u Ly s 1 Al a As n G u ValI Pr a 240 li1e Al a G31y <210O> 483 <21 1> 545 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (522) <223> FRXA00446 <400> 483 at g gaa aag gtt cca aac gat gtc gtt gt t ggg ctg gat gag gct t at I'b t O31u Lys Val Pr o Asn Asp Val Val Val G31y Leu Asp O31u Al a Tyr 1 5 10 t tt gag t tc aac cgc gcg gac gac acc cca gt t gcc act gag gaa at c Phe (31u Phe Asn Ar g Ala Asp Asp Thr Pr o Val Al a Th r 31u 31u Ilie 25 cac cgc cac gac aac gt g at t ggt t tg cgc acg t tc t cc aag gcg t at Hi s Ar g Hi s Asp Asn Val I IIe G31y Leu Ar g Thr Phe Ser Lys Al a Tyr Page 611 seql i st cor r ect ed. t xt 40 ggc ctg gcg ggc ttg cgt gtt ggt tac gcc ttc gga aac gca gag atc 192 Sy Leu Ala y Leu Arg Val y Tyr Ala Phe y Asn Ala Gu lie 55 atc gca gcg atg aat aag gtg gct att cct ttc gcg gtg aat tca gca 240 Ile Ala Ala IVet Asn Lys Val Ala lle Pro Phe Ala Val Asn Ser Ala 70 75 gct cag gcg gca gcg ctt gcg agt ttg aat tct gcc gat gag ttg atg 288 Ala G n Ala Ala Ala Leu Ala Ser Leu Asn Ser Ala Asp G u Leu IVet 90 gaa cgg gtg gag gaa acc gtc gaa aag cgt gat gct gtg gtg tca gcg 336 G u Arg Val G u G u Thr Val G u Lys Arg Asp Ala Val Val Ser Ala 100 105 110 ctt ggt gct gcg ccg acg cag gcc aat ttc gtc tgg ctg ccg ggc gag 384 Leu y Ala Ala Pro Thr G n Ala Asn Phe Val Trp Leu Pro Gy G u 115 120 125 ggc gcc gct gag ttg gcg gct aaa ttg gcc gag cac ggc atc gtg att 432 G y Ala Ala G u Leu Ala Ala Lys Leu Ala G u His G y IIe Val lie 130 135 140 cgc gcg ttc ccc gag ggt gcg cgc att tcg gtg acc aac gcc gag gaa 480 Arg Ala Phe Pro G u G y Ala Arg lie Ser Val Thr Asn Ala G u G u 145 150 155 160 act gac aag ctg ctg cgc gcg tgg gag gcc atc aat gct ggg 522 Thr Asp Lys Leu Leu Arg Ala Trp G u Ala lie Asn Ala y 165 170 tagtctttgg cgttttgcgg tgc 545 <210> 484 <211> 174 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 484 IVet G u Lys Val Pro Asn Asp Val Val Val y Leu Asp G u Al a Tyr 1 5 10 Phe G u Phe Asn Arg Ala Asp Asp Thr Pro Val Ala Thr G u G u Ile 25 His Arg His Asp Asn Val I e y Leu Arg Thr Phe Ser Lys Ala Tyr 40 Sy Leu Ala y Leu Arg Val y Tyr Ala Phe y Asn Ala Gu lie 55 Ile Ala Ala IVet Asn Lys Val Ala lle Pro Phe Ala Val Asn Ser Ala 70 75 Ala G n Ala Ala Ala Leu Ala Ser Leu Asn Ser Ala Asp G u Leu IVet 90 G u Arg Val G u G u Thr Val G u Lys Arg Asp Ala Val Val Ser Ala 100 105 110 Leu y Ala Ala Pro Thr G n Ala Asn Phe Val Trp Leu Pro Gy G u 115 120 125 G y Al a Ala G u Leu Al a Ala Lys Leu Ala G u His G y IIe Val lie Page 612 seqI i st car r ect ed. t xt 130 135 140 Ar g Ala Ph e Pro (31u G31y Ala Arg lieSer Val Thr Asn Ala Ou Ou 145 150 155 160 Thr As p Lys Leu Leu Ar g Ala Tr p G31u Ala Ilie As n Ala G31y 165 170 <210O> 485 <21 1> 1230 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (1207) <223> RXS00618 <400> 485 gct gcat t ag agggt cgt at ct cgat ct aa aagcagt acg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cagat aggct t gt ct ct t at at g cag at g [vb t 0 n db t cga Ar g gct A a gag (31u g at As p g ac As p ggt G31y gt g ValI t ct Ser tt c Ph e gct A a 1 50 ccg Pr o c ac Hi s c ag O31n gct A a gag G31u g ac As p tt c Ph e at g vb t ggg G31y 120 cca Pr o at t I Ie gag (31u cgc Ar g t ca Ser cgc Ar g cgt Ar g aac As n gca A a ac c Thr aag Ly s gct A a ac c Thr gag (31u ag g Ar g act Thr t cg Ser gaa G31u cct Pr o 75 t cg Ser ccg Pr o gt t ValI caa O31n ag c Ser 155 cgc Ar g cgc Ar g ggt G31y ggt G31y cgc Ar g g ac As p tt t Ph e ggg ct g Le u at g vb t 140 cca Pr a at c I Ie g aa O31u gcg A a cct Pr a 45 at c I Ie aat As n at c I Ie t ac Tyr aac As n 125 tt g Le u gga G31y gcc A a ggc G31y cca Pr a tt g Le u gcc A a gt t ValI gcc A a ccg Pr a 110 ct g Le u gag O31u aac As n aag Ly s aaa Ly s 15 g aa O31u gga G31y g at As p at t I Ie ac c Thr 95 gca A a cgc Ar g g aa O31u cca Pr a g ac As p gca A a t ac Tyr t gg Tr p gt c ValI 80 tt g Le u t at Tyr t gt cys ct g Le u ac g Thr 1 60 1 ac c Thr gt c ValI ac c Thr c ac Hi s ac c Thr g at As p cgc Ar g act Thr cca Pr a 145 ggc G31y tt a Le u at c I Ie gag O31u t ct Ser ac c Thr c ac Hi s aat As n gca A a 130 c ac Hi s ac c Thr at g vb t g aa O31u gt g ValI gct A a ggt G31y ggg at t I Ie 115 gag O31u aag Ly s at c I Ie ttg gac Leu Asp t tc tg c Phe Cys gaa gca 03 u A a at t ggt I Ie G31y act t at Thr Tyr t ct t ca Ser Ser gat t at Asp Tyr 1 00 ct g gaa Leu 03 u act cgt Thr Ar g ccg aag Pr o Lys at t gat I I e As p 1 t gg t gc gat gac aat gat gct Tr p Cy s As p As p As n As p Ala Page 613 seql i st cor r ect ed. t xt gtt ctt atc Val Leu II e ct g gca act Leu Al a Thr 200 ttg tcc aag Leu Ser Lys 215 gt t cca gat Val Pro Asp 230 tcc ttg tgt Ser Leu Cys act ttg gag Thr Leu G u gag gcc cgg G u Al a Ar g 280 act ttc gcc Thr Phe Al a 295 gca t ac acc Al a Tyr Thr 310 gcg ggc gtg Al a G y Val cac aag tgg His Lys Tr p gaa ggt gt g G u G y Val 360 gat Asp cat Hi s ttc Phe ct g Leu cct Pr o 250 ggg Sy gt g Val ccg Pr o gat Asp gt c Val 330 cgt Ar g gag G u cag G n t cc Ser gt c Val 235 gcc Al a gcc Al a tt c Phe gat Asp t ca Ser 315 gcg Al a tt g Leu gac Asp ttt Phe at g let 220 aca Thr at c l Ie gaa G u gt c Val ggc G y 300 gag G u ccg Pr o agc Ser cac Hi s 190 aag Lys ggt Sy at t SIle cag G n gat Asp 270 aag Lys ct g Leu tgg Tr p gtt Val tgc Cys 350 175 ggc G y aac Asn tgg Tr p gaa G u gct Al a 255 gcc Al a ct c Leu t at Tyr gca Al a gat Asp 335 gcg Al a at g Met gcc Al a cgc Ar g aac Asn 240 gcg Al a cac Hi s cct Pr o ttg Leu ttg Leu 320 ttt Phe t ca Ser ttt Phe gt g Val 210 ggt Sy cag G n cgc Ar g gaa G u at c SIle 290 gt c Val ttg Leu cct Pr o gaa G u ggt G y 195 gt g Val tgg Tr p gct Al a gca Al a gcg Al a 275 ggg G y gat Asp ct c Leu gag G u gac Asp 355 cgt Ar g ggt Sy at c SIe tct Ser gcc Al a 260 t at Tyr ctt Leu gtt Val gat Asp gaa G u 340 acc Thr 691 739 787 835 883 931 979 1027 1075 1123 1171 1217 1230 aaa at c gga Lys I I e y ttc atc aaa aaa tagcagcgac Phe I e Lys Lys taggttagtt tcg <210> 486 <211> 369 <212> PRT <213> Corynebacter i um gl utam cum <400> 486 lIet G n let Leu Asp Arg Val His Arg Arg Arg Arg G u y Lys Asp 1 5 10 Thr Leu IVet Phe Cys Al a Gy n Pro Ser Thr y Ala Pro G u Ala 25 Val I e u u Ala u Ile Ala Leu Arg Ser y Pro Leu y Tyr 40 Page 614 seqi i st car r ect ed. t xt Th r (31u Val I IIe G31y As p Ar g (31u Ph e Ar g (31u Ar g 1 1 55 e Ala Asp Tr p Hi s Thr As p Ar g Thr Pr o 145 G31y As p Ser I Ie ValI 225 Le u G31y ValI (31u Tr p 305 Ar g As p Ly s Ly s A a G31y Gy le 115 (31u Ly s I Ie As n G31y 1 95 ValI Tr p A a A a A a 275 G31y As p Le u O31u As p 355 Tyr Ser Tyr O31u Ar g Ly s As p 1 65 A a Pr o Thr I Ie Le u 245 Ph e Ar g G31y Ser O31u 325 G31y I Ie As p 70 G31y ValI Ser Ph e A a 150 Pr o ValI Le u Le u ValI 230 Ser Thr O31u Thr A a 310 A a Hi s (31u ValI G31y A a Le u O31n 135 ValI (31u Le u A a Ser 215 Pr o Le u Le u A a Ph e 295 Tyr G31y Ly s G31y Thr ValI Pr a 1 05 A a Thr ValI Le u Ser 1 85 A a Tyr (31u A a A a 265 (31u As p As p A a I Ie 345 Ar g As n A a 90 Thr Ly s A a Thr (31u 1 70 As p Hi s Ph e Le u Pr a 250 G31y ValI Pr a As p ValI 330 Ar g Ly s Pr a 75 Ser Pr a ValI O31n Ser 1 55 Ar g O31u O31n Ser ValI 235 A a A a Ph e As p Ser 315 A a Le u I Ie As n I Ie Tyr As n 125 Le u G31y A a Tyr Ser 205 Thr Pr a G31y Le u As p 285 O31y O31u G31y Le u O31u 365 ValI A a Pr a 110 Le u O31u As n Ly s Hi s 1 Ly s Gy le O31n As p 270 Ly s Le u Tr p ValI cys 350 Ph e I Ie Thr A a Ar g O31u Pr a Tr p 1 O31y As n Tr p O31u A a 255 A a Le u Tyr A a As p 335 A a I Ie ValI Le u Tyr cys Le u Thr 1 cys A a Ar g As n 240 A a Hi s Pr a Le u Le u 320 Ph e Se r Ly s <210O> 487 <21 1> 657 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 615 seqI i st car r ect ed. t xt <220> <221 OIDS <222> (634) <223> FRXA00618 <400> 487 cccaacgggc accatcattg atccggaaga gctagagcgc caat gat gct gt t ct t at ct ct gat gagga ct accacggc at cgccaagt ggt gcgat ga at g agc t tt ggt I'bt Ser Phe G31y ccg Pr o ac c Thr at c I Ie ct t Le u tt c Ph e cgc Ar g ggC G31y t ct Ser g aa O31u G31y 1 50 at t I Ie gca A a t cc Ser cca Pr o tt g Le u tt g Le u gcc A a tt c Ph e t ac Tyr 120 ggC G31y aag Ly s ggt G31y gcg A a t ac Tyr gag O31u gct A a gct A a gag O31u g ac As p g at As p gcc A a at t I Ie C ag O31n t cc Ser gt c ValI gcc A a gcc A a tt c Ph e g at As p t ca Ser gcg A a 140 tt g Le u t cc Ser ac g Thr ccg Pr o ggg G31y ct t Le u g at As p ggc G31y 110 g aa G31u ggt G31y ct g Le u aac As n t gg Tr p g aa G31u gct A a gcc A a ct c Le u t at Tyr gca A a g at As p gcg A a 1 1 gcc A a cgc Ar g aac As n gcg A a c ac Hi s cct Pr o tt g Le u tt g Le u tt t Ph e 145 t ca Ser at c I Ie gt g ValI ct g Le u gga G31y gt t ValI g aa G31u t gg Tr p cgt Ar g 130 g at As p aag Ly s gt g ValI ggt G31y c ag O31n cgc Ar g g aa O31u at c I Ie gt c ValI 115 tt g Le u cct Pr a g aa (31u gt g ValI t gg Tr p gct A a gca A a gcg A a ggg G31y 1 00 g at As p ct c Le u gag O31u g ac As p cgc aaa at Ar g Lys 1 1 1 70 c gga gaa ttc atc aaa aaa e 0 y 0u Phe Il e Lys Lys 1 t agcagcgac t aggt tagt t t cg <210O> 488 <211> 178 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 488 N/t Ser Phe (31y Ar g Pro Leu Ala Thr Ala Hi s (31n Phe Ser Lys Asn 1 5 10 Page 616 Al a Ar g Asn Al a Hi s Pr o Leu Leu Phe 145 Ser Lys Val Gy G n Ar g G u SIle Val 115 Leu Pr o G u Gy I e Ser Al a Tyr Leu Val Asp G u Thr 165 Leu Val Ser Thr G u Thr Al a Al a 135 Hi s G u seql i st cor r ect ed. t xt Ser Lys Tyr Phe Ser IVet Pro Asp G u Leu Val Thr 40 Leu Cys Ala Pro Ala lie Leu u Al a y Ala G u 75 Ala Arg G u Val Phe Val 90 Phe Ala Asp Pro Asp G y 105 Tyr Thr Asp Asp Ser G u 120 125 Sy Val Ala Val Ala Pro 140 Lys Trp Ile Arg Leu Ser 155 Sy Val Arg Lys Ile y 170 Gy I e G n Asp Lys Leu Tr p Val Cys Phe 175 Tr p G u Al a Al a Leu Tyr Al a Asp Al a 160 I e <210> 489 <211> 385 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(385) <223> FRXA00627 <400> 489 gctgcat t ag agggtcgtat ctcgatctaa aag gaagccaagc actagaagca atgttcagcc gtt icagtacg cagataggct tgtctct t at tcgcgtc atg cag atg ttg gac IVet G n IVet Leu Asp 1 aaa gac acc tta atg ttc tgc Lys Asp Thr Leu IVet Phe Cys 15 gaa gca gtc atc gaa gaa gca Su Ala Val Ile G u G u Ala gga tac acc gag gtg att ggt Sy Tyr Thr u Val Ile y gat tgg cac tct gct act tat Asp Trp His Ser Ala Thr Tyr Page 617 cga Ar g gct Al a gag G u gat Asp cac Hi s cag G n gct Al a gag G u cgc Ar g t ca Ser cgc Ar g cgt Ar g agg Ar g act Thr tcg Ser gaa G u cgc Ar g ggt Gy ggt Gy cgc Ar g gaa G u gcg Al a cct Pr o 45 at c SIle ggc Gy cca Pr o ttg Leu gcc Al a seql i st cor r ect ed. t xt gac gta gac acc aac cct gac aat gtt att gtc acc acc ggt tct tca 355 Asp Val Asp Thr Asn Pro Asp Asn Val lie Val Thr Thr y Ser Ser 75 80 ggt gga ttc gtg gca tcg ttt atc gcc acc 385 G y G y Phe Val Al a Ser Phe I e Ala Thr <210> 490 <211> <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 490 IVet G n Iet Leu Asp Arg Val His Arg 1 5 Thr Leu IVet Phe Cys Al a Gy n Pro 25 Val Ile G u u Ala u Ile Ala Leu 40 Thr u Val I e y Asp Arg u Phe His Ser Ala Thr Tyr Asp Val Asp Thr 70 Thr Thr G y Ser Ser G y G y Phe Val Ar g 10 Ser Ar g Ar g Asn Al a 90 Ar g Thr Ser G u Pr o Ser G u Al a Pr o I le Asn SIle Gy Pr o Leu Al a Val Al a Lys G u Gy Asp SIe Thr <210> 491 <211> 1221 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1198) <223> RXS01105 <400> 491 ttgtcatcaa ctttgccaac gccgaagatc ttccagcgca gctttgaaaa cctccccacc accgacgagg cctaagaaaa ttg Leu ggc Gy cct Pr o gat Asp gaa gat Asp ccc Pr o cca Pr o at c SIle cgt cca Pr o ct c Leu t ca Ser acc Thr gag cgt Ar g gtt Val gca Al a ct g Leu gct gaa G u gat Asp ttg Leu 45 aac Asn gcg gaa G u at t I e gt c Val cgc Ar g t ac ctg cgc Leu Arg 15 cgc ct c Arg Leu gct gac Al a Asp tac cca Tyr Pro atc acc Page 61 cggcgaagca atccgtgcac atg acc aaa att act IVet Thr Lys Ile Thr 1 ggt gag cac gct tac G y G u Hi s Al a Tyr aac acc aac gaa aac Asn Thr Asn G u Asn ttg gtt gcc acc gtg Leu Val Ala Thr Val gag cgc gat gct gtg G u Arg Asp Ala Val aag caa acc ggc gtg G u Leu Ar g Asp G u Leu Ala Ala eql i st cor r ect ed. t xt Tyr Ile Thr Lys n Thr G y Val gct Al a ct g Leu gga Gy cac Hi s at g let 150 ttt Phe gat Asp gaa G u gag G u ttt Phe 230 ttt Phe ct g Leu ct g Leu cgc Ar g gt g Val 310 ttg Leu acc Thr cag G n caa G n 120 gaa G u gt g Val acc Thr gag G u t at Tyr 200 t ac Tyr ttc Phe gac Asp caa G n acc Thr 280 gag G u ttt Phe agg Ar g gat Asp ct g Leu agc Ser at t SIle ct g Leu ccg Pr o 170 at c SIle gaa G u acc Thr ggt Sy gt g Val 250 gcc Al a gaa G u ct g Leu gat Asp gt g Val 330 aac Asn cag G n t at Ty r gcg Al a gaa G u 155 aac Asn at c SIle tt c Phe aag Lys gga G y 235 at g Met gca Al a aag Lys ggc Gy tt c Phe 315 ct c Leu ct g Leu gct Al a t cc Ser gt g Val 140 gaa G u aac Asn aac Asn t cc Ser ct g Leu 220 cgc Ar g ct a Leu at c l Ie ct c Leu t ac Tyr 300 t cc Ser at c SIle tgg Tr p ttt Phe at g Met 125 t cc Ser at t SIle ccg Pr o gtt Val cca Pr o 205 gt g Val ct c Leu gt c Val gt a Val tct Ser 285 gct Al a gat Asp cgc Ar g gct Al a ggt Gy 110 cac Hi s cga Ar g cgt Ar g acc Thr gcc Al a 190 t ca Ser gt g Val ggc G y cgc Ar g gcg Al a 270 gt a Val gt g Val cag G n gat Asp gcc Al a 95 gga G y cca Pr o ggt G y gca Al a ggt G y 175 cca Pr o cct Pr o t cc Ser t ac Tyr ctt Leu 255 ct g Leu gag G u gtt Val cac Hi s gt g Val 335 aat Asn cct Pr o at t SIle gct Al a aag Lys 160 gat Asp ggc G y t ca Ser cgc Ar g ttc Phe 240 ccg Pr o cgt Ar g cgt Ar g cca Pr o gcg Al a 320 gga G y ggt G y gga G y ttg Leu gat Asp 145 cag G n gt g Val at c SIle gca Al a acc Thr 225 gt g Val t at Tyr cac Hi s gtt Val agt Ser 305 gca Al a at c SIle t cc Ser cgc Ar g gct Al a 130 ttc Phe cct Pr o acc Thr gt g Val acc Thr 210 at g Met gcc Al a cat Hi s t cc Ser cgc Ar g 290 gag G u tgg Tr p gct Al a gaa G u 100 gcg Al a ggc G y at c SIle at t SIle ct g Leu 180 gt g Val ctt Leu aag Lys cca Pr o t ca Ser 260 gac Asp gca Al a aac Asn gca Al a cac Hi s 340 at t SIe ttg Leu acc Thr gat Asp gtt Val 165 gac Asp gat Asp ct g Leu gct Al a gcg Al a 245 gcg Al a acg Thr gca Al a ttt Phe ttt Phe 325 ttg Leu 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 cgc act acc att ggt gtg cct gag gaa aat gat gcg ttt ttg gac gca Page 619 seql i st cor r ect ed. t xt Arg Thr Thr Ile Gy Val Pro G u u Asn Asp Ala Phe Leu Asp Ala 345 350 355 gct gca gag atc atc aag ctg aac ctg taagagagaa gaatttttca Ala Ala G u Ile Ile Lys Leu Asn Leu 360 365 tga <210> 492 <211> 366 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1218 1221 <400> 492 IVet Thr Lys 1 Gy Asn Leu G u Lys Gy Gy Leu Asp 145 G n Val SIle Al a Thr 225 Val Tyr Hi s Asn Al a Asp Thr Asn Thr 115 Lys Ar g Asp Ser SIle 195 Thr Ser Asn Leu I e Thr Leu 5 Al a Tyr G y G u Asn Pr o Thr Val Asp Ala Val u 70 Sy Val Al a G u Ile Leu 100 Al a Leu G y G y Thr Hi s Ile Asp Ibet 150 Ile Val Phe 165 Leu Asp Asp 180 Val Asp G u Leu Leu G u Lys Ala Phe 230 Pro Al a Phe 245 Ser Al a Leu 260 Ser Al a Tyr Lys Leu Val G n Phe Thr 135 Asp Val Val Al a Lys 215 Asp SIle Ser Leu Pro 10 G n Leu 25 Pro Ser Al a Thr Asp u Arg Asp 90 Leu Leu 105 Pro Ser Phe Il e Al a Leu Thr Pro 170 Arg Ile 185 Al a G u Pro Thr Al a G y Al a Val 250 Al a Al a 265 Leu Arg G u G u Leu Arg Asn G u G u Leu Asn G n Tyr Al a G u 155 Asn SIle Phe Lys Gy 235 Met Al a Val Al a Leu Al a Leu Al a Ser Val 140 G u Asn Asn Ser Leu 220 Ar g Leu l Ie Asp Leu Asn Al a Tr p Phe bet 125 Ser SIle Pr o Val Pr o 205 Val Leu Val Val I e Val Ar g Tyr Al a Gy 110 Hi s Ar g Ar g Thr Al a 190 Ser Val Gy Ar g Al a 270 Ar g Al a Tyr SIe Al a Gy Pr o Gy Al a Gy 175 Pr o Pr o Ser Tyr Leu 255 Leu His Ser Ala Asp Thr Leu G y Thr Val G u Lys Leu Ser Val G u Arg Page 620 Val Arg 290 Ser G u 305 Al a Tr p I e Al a Al a Phe 275 Val Ala Ala Arg Leu 295 Ser Asn Phe Val Phe 310 G n Ala Phe Leu Asp 325 G y His Leu Arg Thr 340 Leu Asp Al a Al a Al a 355 seql i st cor r ect ed. t xt 280 285 G u G u Leu G y Tyr Al a 300 Phe G y Asp Phe Ser Asp 315 Arg G y Val Leu lie Ar g 330 Thr I I e G y Val Pro G u 345 G u i e i e Lys Leu Asn 360 365 Val Val Pr o G n His Ala 320 Asp Val G y 335 G u Asn Asp 350 Leu <210> 493 <211> 1752 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1729) <223> RXS02315 <400> 493 cgtttggaaa cgcttgctgc cagcaaagat aggcgtgatt gaatcgtatc cgtacctgga gacgatctag actgttgtgc ggtggtttga gcgcgtgcgt atg tcc agc acg cca 11! IVet Ser Ser Thr Pro gct Al a act Thr gag G u cgc Ar g ccg Pr o cct Pr o tct Ser at t SIle gat Asp gt g Val ct g Leu gcc Al a gct Al a gtt Val gac Asp cag G n 120 gcc Al a tta Leu cgg Ar g ttt Phe gt g Val gaa G u cct Pr o ggt G y cgc Ar g tgc Cys cag G n tt g Leu at g Mbt 75 gct Al a gca Al a at t SIle gcc Al a cca Pr o gat Asp gcg Al a acc Thr gcg Al a cat Hi s ttt Phe gtt Val gga G y ct g Leu 45 ct g Leu t cc Ser cat Hi s ttg Leu ggt 15y 125 at t SIle t cc Ser cgt Ar g t cc Ser ggc G y gcc Al a gt g Val 110 gat Asp gat Asp 15 agg Ar g gt c Val ct a Leu acg Thr cat Hi s 95 gga G y ctt Leu t cc Ser aac Asn cat Hi s gcg Al a gct Al a at c SIle acg Thr gca Al a gca Al a ccg Pr o cgt Ar g acc Thr gct Al a ttg Leu gcg Al a acg Thr 130 cca Pr o ttg Leu at c SIle cag G n aac Asn at t SIle agc Ser 115 gt c Val cac Hi s tcg Ser gac Asp gcc Al a tgc Cys gtg Val 100 caa G n ggt G y act gag ctg gat cag gta gcg cag att gct gaa agc ctt gct cag ggg Page 621 seqi i st car r ect ed. t xt Thr Ou Leu Asp On Val Ala O1n lie Ala Olu Ser Leu A a G3 n G3 y 135 gct A a 1 50 gt t ValI t gg Tr p gag O31u ct g Le u aat As n 230 t cc Ser gt g ValI cct Pr o ccc Pr o ac c Thr 310 gcg A a ac c Thr g at As p gt g ValI gca A a 390 c ag O31n cct Pr o cat Hi s ac c Thr 200 g at As p gt g ValI g aa O31u c ac Hi s at t I Ie 280 cgc Ar g g aa O31u g at As p ct c Le u ct c Le u 360 at g vb t at t I Ie ccg Pr o ct g Le u 1 70 t gg Tr p gt g ValI ccc Pr a cca Pr a t at Tyr 250 ac g Thr tt a Le u gcc A a c ag O31n gt g ValI 330 gt t ValI gct A a tt t Ph e ggt O31y cgt Ar g 155 cca Pr a at c I Ie at t I Ie acc Thr ct g Le u 235 gt g ValI ct g Le u act Thr g at As p t gg Tr p 315 cgt Ar g gcc A a gca A a g at As p t ct Ser 395 140 cat Hi s gag O31u aac As n gcc A a at c I Ie 220 gct A a gt a ValI c ac Hi s gt g ValI c ag O31n 300 ct a Le u g ac As p gca A a gca A a ggc O31y 380 gt t ValI tt c Ph e ct t Le u c ac Hi s ggt O31y 205 gct A a gct A a aac As n cgc Ar g ct t Le u 285 gt g ValI aag Ly s gt c ValI gcc A a t cc Ser 365 gt g ValI gct A a aat As n cat Hi s ggt O31y 1 90 g at As p g aa O31u g aa O31u ac c Thr gga O31y 270 t ca Ser ggc O31y at c I Ie ct g Le u gt g ValI 350 aac As n g at As p c aa O31n ct t Le u ggt O31y 1 75 gag O31u g aa O31u cct Pr a at c I Ie ag g Ar g 255 gt g ValI cgc Ar g ag c Ser t gt cys g ac As p 335 gcg A a t ca Ser ac c Thr gca A a gca A a 1 60 gag O31u gt g ValI gca A a act Thr tt g Le u 240 cct Pr a tt g Le u ac c Thr ac a Thr t cg Ser 320 aac As n g at As p at c I Ie tt c Ph e at c I Ie 400 145 ct t Le u gca A a ac c Thr t gg Tr p gca A a 225 ct g Le u g at As p aag Ly s g at As p gt g ValI 305 gca A a c aa O31n ac c Thr cgt Ar g t cc Ser 385 ggc O31y g at As p gt t ValI gt g ValI g aa O31u 210 cca Pr a aag Ly s cat Hi s tt g Le u at c I Ie 290 aaa Ly s gca A a g aa O31u tt a Le u g ac As p 370 cca Pr a act Thr gt t ValI gga O31y g ac As p 1 gt g ValI aag Ly s gag O31u gt g ValI at g vb t 275 at c I Ie gt c ValI t ca Ser tt c Ph e ggc O31y 355 ct c Le u cga Ar g t ca Ser cct Pr a gca A a 1 ct g Le u g aa O31u cct Pr a c ag O31n at c I Ie 260 t ca Ser act Thr ac c Thr g aa O31u ggt O31y 340 ac c Thr t cc Ser ggt O31y ct t Le u 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 gtg cag tcc cgc cac ccc gat gaa atc cgc gcg cca cgc act gtg gcc Page 622 Val n Ser Arg His 410 Pro Asp G seql i st cor r ect ed. t xt u lie Arg Ala Pro Arg Thr 415 Val Ala 420 ctt ctg ggc gat ctg tcg ttc ctt cac Leu Leu G y Asp Leu Ser Phe Leu Hi s 425 430 ggc cct gat gaa cca cgc cca gaa aac G y Pro Asp G u Pro Arg Pro G u Asn 440 445 gac aac ggt ggc gga atc ttc gaa ctc Asp Asn y y y Ile Phe u Leu 455 460 ctc cgc ccc aac ttc gag cgt gct ttc Leu Arg Pro Asn Phe G u Arg Ala Phe 470 475 atc gcg gat ctc tgc gca ggc tac ggc ile Al a Asp Leu Cys Al a G y Tyr G y 490 aac ctc caa gac ctc atc atc gcg ct a Asn Leu G n Asp Leu Ile Ile Ala Leu 505 510 gga ttc acc att att gaa gct tcg acc Gy Phe Thr Ile Ile u Ala Ser Thr 520 525 caa cag caa gct ctc atg gac acg gtg G n G n G n Ala Leu bet Asp Thr Val 535 540 gcg <210> 494 <211> 543 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 494 Met Ser Ser Thr Pro Al a n Asp Leu 1 5 Leu Ala Pro His Val Thr Asp Val Val 25 Ser Pro Leu Ser Leu G u Leu Leu Ala 40 Val Arg Ile Asp G u Arg Ser Ala Ser 55 Arg Thr G n Ala Arg Pro Val Ala Val 70 Val Ala Asn Cys Leu Pro Ala Val Ala Pro Leu lie Val Leu Ser Ala Asp Arg 100 105 Sy Ala Ser n Thr lie Asn n Thr 115 120 at t SIle acc Thr gaa Gu acc Thr 480 gaa G u gat Asp cga Ar g gga ct g G y Leu 435 gtg gt c Val Val 450 ggc gca Sy Al a cac gac His Asp caa gt g G n Val acc gaa Thr G u 515 acc cgc Thr Arg 530 ct c Leu t cc Ser gat Asp gcg Al a gt a Val 500 gt a Val cgt Ar g at c SIe aac Asn ggt Gy t cc Ser 485 gac Asp t cc Ser gca Al a 1411 1459 1507 1555 1603 1651 1699 1749 1752 cac Hi s taaatggagt ggtaccaagt Al a Ar g Al a 10 Leu Cys Pro Arg n Asp Phe Leu Al a Val Met Thr G u Ala Al a 90 Pro Al a Hi s G y Ile Phe Page 623 Val Gy Leu Leu Ser Hi s Leu Gy 125 SIle Ser Ar g Ser Gy Al a Val 110 Asp Asp Ar g Val Leu Thr Hi s Gy Leu seqI i st car r ect ed. t xt Pro Thr Val Gy lieThr Ou Leu Asp On Val Ala O1n 130 135 140 I I e A a (3 u Ser 145 Le u A a Thr Tr p A a 225 Le u As p Ly s As p ValI 305 A a O31n Thr Ar g Ser 385 G31y Pr o G31y I Ie Thr 465 Pr o A a ValI G31y As p 1 95 ValI Ly s O31u ValI vb t 275 li1e ValI Ser Ph e G31y 355 Le u Ar g Ser Thr Le u 435 ValI A a As p G31y Le u 1 65 Ser G31y G31y Tyr ValI 245 ValI As p As p G31y Le u 325 Ph e G31y Le u ValI A a 405 A a I Ie As n G31y Ser 485 A a 150 ValI Tr p O31u Le u As n 230 Ser ValI Pr o Pr o Thr 310 A a Thr As p ValI A a 390 ValI Le u G31y As p Le u 470 li1e Ser A a Thr Hi s O31u 215 Pr a A a G31y G31y G31y 295 O31n A a O31y Thr O31y 375 O31y O31n Le u Pr a As n 455 Ar g A a I Ie O31u Ar g 1 Le u ValI Hi s O31y Pr a 265 Ly s Hi s Ly s O31y Hi s 345 Ph e Pr a As p Ar g As p 425 O31u O31y As n Le u Pr a Le u 1 70 Tr p ValI Pr a Pr a Tyr 250 Thr Le u A a O31n ValI 330 ValI A a Ph e O31y Hi s 410 Le u Pr a O31y Ph e Cys 490 Ar g 1 Pr a I Ie I Ie Thr Le u 235 ValI Le u Thr As p Tr p 315 Ar g A a A a As p Ser 395 Pr a Ser Ar g I Ie O31u 475 Hi s O31u As n A a I Ie 220 A a ValI Hi s ValI O31n 300 Le u As p A a A a O31y 380 ValI As p Ph e Pr a Ph e 460 Ar g Ph e Le u Hi s O31y 205 A a A a As n Ar g Le u 285 ValI Ly s ValI A a Ser 365 ValI A a O31u Le u O31u 445 O31u A a Le u O31y 1 O31u O31u Pr a I Ie Ar g 255 ValI Ar g Ser cys As p 335 A a Ser Thr A a Ar g 415 As p Le u Le u (31y Al a 0 y Tyr 0 y Ile 0 u 495 Page 624 seqI i st car r ect ed. t xt Hi s G3 n ValI Val Asp Asn Leu G3 n Asp Leu I I e I I e A a Leu Val Asp 500 505 510 Thr Thr O31u Val Ser G31y Phe Thr I Ie Ilie Ou Ala Ser Thr Val Ar g 515 520 525 Asp Thr Ar gAr gA a(1n (1n (1n A a Leu Ibt Asp Thr Val His 530 535 540 <210> 495 <211> 1434 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (1411) <223> RXS02550 <400> 495 gat ct taggc agccgt ggga t tacaccct t t tagagct ag aacagt aaaa at tcacccaa t agct t tcaa ct acgcacac aaagt ggcaa cat tgagcgg act aca gac Thr Thr Asp cgc Ar g g at As p c ag O31n gcc A a ct c Le u at t I Ie ac c Thr g aa (31u G31y aac As n 1 50 ac c Thr gca A a gag (31u gag (31u ac g Thr cgt Ar g aaa Lys5 gt g ValI 120 ggt G31y G31y aag Lys5 cgt Ar g at g vb t g aa (31u aat As n at g vb t at t I Ie gga G31y t ca Ser g aa (31u acc Thr ccc Pr o aag Lys5 cgc Ar g cca Pr o 75 at c I Ie at t I Ie t t c Ph e gaa (31u gt t ValI 155 ac c Thr act Thr g ac As p at g vb t gcc A a gcc A a ccg Pr o ccc Pr o ct a Le u 140 ct t Le u ac c Thr cga Ar g ct g Le u ct t Le u tt c Ph e ct t Le u cgg Ar g 110 tt c Ph e ac c Thr ccc Pr o gcc A a 15 ac a Thr t ac Tyr g at As p gga G31y cca Pr a 95 cga Ar g g at As p at g vb t gca A a aac As n act Thr gag (31u ggg G31y tt c Ph e act Thr gca A a gt t ValI ac c Thr ccg Pr a 1 gct A a cgc Ar g cgt Ar g aac As n gcc A a c aa O31n gt c ValI g at As p 130 c aa O31n t ac Tyr gt g ValI at c I Ie ggc G31y at c I Ie ccc Pr a ggg G31y ac c Thr 115 gt g ValI gca A a cca Pr a ggc G31y tt c Ph e ccg Pr a tt a Le u g ac As p t at Tyr 1 00 cgc Ar g tt c Ph e ct c Le u ct g Le u Page 625 act gcc gca acc tcc ctg gct gg Thr Ala Ala Thr Ser Leu Ala 0 seql i st cor r ect ed. t xt t ggt aag cct gtg ca y y Lys Pro Val Hi c tac ct c s Tyr Leu gat Asp at c SIle acg Thr gca Al a 230 at t SIle gat Asp gca Al a gca Al a tgc Cys 310 cgc Ar g cag G n tgt Cys aac Asn cgt Ar g 390 cat Hi s gaa G u gaa G u gag G u 200 gct Al a gag G u t ac Tyr ctt Leu t ac Tyr 280 gga Gy aat Asn t cc Ser aac Asn aaa Lys 360 t ac Tyr gag G u gat Asp gca Al a 170 gac Asp acc Thr t ac Tyr gac Asp gat Asp 250 at c SIle gct Al a at t SIle cca Pr o t ac Tyr 330 gca Al a at g lbt at c SIle at c SIle ttc Phe 410 gag G u tgg Tr p aaa Lys ccg Pr o ct g Leu 235 gcc Al a aca Thr ggc Gy gag G u gct Al a 315 gac Asp tgg Tr p gga Gy cac Hi s ct c Leu 395 cga Ar g cgc Ar g aac Asn gct Al a cgc Ar g 220 ct g Leu gag G u t ac Tyr tgg Tr p ggc Gy 300 cag G n ct c Leu acg Thr gct Al a gac Asp 380 at g Met gt g Val ct g Leu cca Pr o at t l Ie 205 cgg Ar g at t SIle cac Hi s aac Asn at g Met 285 ct c Leu cac Hi s act Thr aaa Lys ct a Leu 365 gac Asp gtt Val gt c Val ggt Gy t cc Ser 190 gt g Val gt g Val ttg Leu at c SIle ggt Gy 270 gt a Val gaa G u gct Al a ggc Gy ct c Leu 350 t ac Tyr acc Thr cag G n acc Thr aac Asn 430 at c SIle gt g Val ttg Leu gcc Al a agc Ser 255 ct a Leu ttg Leu ct c Leu at t l Ie gaa G u 335 aac Asn gcg Al a caa G n ggc Gy ct g Leu 415 ttc Phe gaa G u at c SIle gaa G u gat Asp 240 ct g Leu t cc Ser act Thr ct c Leu cag G n 320 cac Hi s gaa G u ttc Phe ct c Leu act Thr 400 cca Pr o ct g Leu gac Asp aac Asn caa G n 225 gaa G u gca Al a aag Lys gga Sy gca Al a 305 gt a Val ggc Sy at c SIle ccc Pr o at g Met 385 ggc G y tgg Tr p t cc Ser at c SIle ccc Pr o 210 at c SIle at c SIle acc Thr gca Al a cca Pr o 290 ggc Sy gct Al a cga Ar g cca Pr o aag Lys 370 ct g Leu ttc Phe gca Al a act Thr 180 t cc Ser aac Asn gag G u gac Asp gca Al a 260 cgc Ar g caa G n cga Ar g ggt Sy ct g Leu 340 gt c Val gac Asp ctt Leu tgg Tr p cag G n 420 aag Lys tgt Cys aaa Lys ccc Pr o at t SIe cgc Ar g 245 cca Pr o gt c Val t ac Tyr ct c Leu gga Sy 325 gaa G u agc Ser ccc Pr o ct c Leu cca Pr o 405 ttg Leu cag G n 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 Page 626 seql i st cor r ect ed. t xt tagtagttgt taggattcac cac <210> 496 <211> 437 <212> PRT <213> Corynebacter i um gl ut am cum 1434 <400> 496 Val Thr Thr Asp 1 Lys Ala Val y Arg Arg lie Phe lie Arg Gy Pro His Asn Ile Leu Asp Ala Pro Asp Ser G n y Tyr 100 Val Val Thr Arg 115 Asp Asp Val Phe 130 Thr G n Ala Leu 145 Asp Tyr Pro Leu Val His Tyr Leu 180 Asp Ile Lys Ser 195 Asn Pro Asn Asn 210 Gn Ile Val u 225 G u Ile Tyr Asp Ala Thr Leu Al a 260 Lys Ala Tyr Ar g 275 Sy Pro Lys G n 290 Al a G y Thr Ar g 305 Lys 5 Al a Asp Val Lys Val Ser Tyr Leu Leu Tr p 165 Cys Lys Pr o SIle Ar g 245 Pr o Val Tyr Leu Arg Lys Thr Ser Asp G n Al a Leu 70 I le Thr G u Sy Asn 150 Thr Asp SIle Thr Al a 230 I le Asp Al a Al a Cys 310 G n Ser Al a 55 Asn Met Ser Val Asn 135 Asp Al a G u Ser Sy 215 Ar g Leu Leu Sy Ar g 295 Pr o Al a G u 40 G u Thr Ar g Lys Val 120 Sy Sy Al a G u G u 200 Al a G u Tyr Leu Tyr 280 Sy Asn Al a Lys Al a Sy Asp Sy 105 Pr o Val Asp Thr Asp 185 Lys Val Hi s Asp Cys 265 Ar g Phe Val Thr Pr o Lys Ar g Pr o I le SIle Phe G u Val 155 Leu Tr p Lys Pr o Leu 235 Al a Thr Sy G u Thr Thr Asp bet Al a Al a Pr o Pr o Leu 140 Leu Al a Asn Al a Ar g 220 Leu G u Tyr Tr p Sy 300 Asp Ar g Val G u Val Asn Al a Hi s 125 I e I e Gy Pr o l Ie 205 Ar g SIle Hi s Asn Met 285 Leu Al a Thr Tyr Asp Sy Pr o Ar g Asp bet Al a Lys 175 I e Val Leu Al a Ser 255 Leu Leu Leu Pro Ala n His Ala lie n 315 320 Page 627 ValI G31y I Ie Pr a vb t 385 G31y Tr p Ser Le u Le u G31y 355 Le u As p As n Ser Tyr 435 G31y 325 O31u Ser Pr a Le u Pr a 405 Le u O31n O31n Ar g ValI ValI 375 A a Hi s As n seql i st car r ect ed. t xt Ser I Ie Tyr Asp Leu Thr 330 As n l'bt Ala Tr p Th r Lys 345 Lys Pr o 'b t G31y Ala Leu 360 365 Ty r 03 u I I e Hi s As p As p 380 03 u Lys I I e Leu l'b t Val 395 As p Hi s Phe Ar g Val Val 410 Ala I Ie O31u Ar g Leu G31y 425 O31u 335 As n A a O31n G31y Le u 415 Ph e <210O> 497 <21 1> 1080 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221 CDS <222> (1057) <223> RXS02319 <400> 497 at gt gggt ga gat aaccgac cgt gat gt cg ccct agcaaa agacct tggc cat t tcggca gaggct taag gt taaagat t agt cat cgac gcccacgcca agc aac t ac agc Ser Asn Tyr Ser ac c Thr g aa O31u gcc A a gct A a gcg A a ccc Pr a aac As n tt c Ph e ggc G31y cgc Ar g cgg Ar g g aa O31u tt t Ph e g ac As p gt g ValI c ac Hi s cca Pr a g ac As p ccc Pr a ac c Thr at c I Ie gt c ValI gt t ValI G31y ac c Thr t ac Tyr gcc A a 45 g ac As p gga G31y t gg Tr p c aa O31n c ac Hi s tt c Ph e gag O31u ac c Thr gcg A a gcc A a c ac Hi s cgc Ar g t ac Tyr ct g Le u 80 t gc Cys gt t ValI ggc G31y g aa O31u 50 gcc A a ac c Thr ggc G31y cca Pr a ac c Thr 35 gt t ValI ct c Le u ggc G31y ggc G31y ggt G31y ac c Thr cgc Ar g g ac As p aac As n g ac As p 1 00 tt t Ph e cgc Ar g aat As n c ac Hi s ggc G31y c aa O31n cgc atc cgc ggg cgc tcc ggc tac caa tac gcc acc gaa cac gcg cgc Page 628 Arg lie Arg y Arg 105 seql i st cor r ect ed. t xt Ser G y Tyr G n Tyr Al a Thr G u 110 Hi s Al a Ar g 115 gac Asp gtt Val acc Thr 150 ggt Gy caa G n gct Al a gcc Al a caa G n 230 aaa Lys ggg G y at g Met acg Thr ccc Pr o 310 aaa gcc Al a 120 ggc Gy cct Pr o cac Hi s gca Al a t ac Tyr 200 gaa G u at g Met gcc Al a cgc Ar g caa G n 280 gaa G u tgg Tr p gct Al a cgc Ar g gtt Val ct c Leu 170 ttc Phe t cc Ser ttc Phe gca Al a caa G n 250 ct g Leu gt c Val gt a Val gat Asp ct c Leu gt c Val 155 cat Hi s aag Lys gcc Al a tt c Phe gt c Val 235 gca Al a aaa Lys tt c Phe gag G u gt c Val cac Hi s 140 at c SIle gt c Val caa G n t ac Tyr ct c Leu 220 aac Asn gcc Al a ttc Phe gcc Al a ggt G3y 300 ttc Phe 125 at t SIle gca Al a gtt Val acc Thr ct a Leu 205 gga G y at c SIle cgc Ar g gcc Al a ggc G y 285 aag Lys acg Thr ttg Leu gt a Val tgc Cys gac Asp 190 gcg Al a cgc Ar g gt g Val gaa G u ttc Phe 270 gaa G u gaa G u gt a Val gaa G u gt c Val gac Asp 175 gct Al a aaa Lys acc Thr gcc Al a at c SIle 255 aat Asn gcc Al a gca Al a gat Asp gt c Val aac Asn 160 ct c Leu gac Asp at g Met t ac Tyr gac Asp 240 aac Asn ct c Leu acc Thr ttc Phe at t SIle caa G n 145 ggc G y acc Thr gt g Val gt c Val gac Asp 225 cac Hi s acc Thr acc Thr cgc Ar g ct a Leu 305 gcc Al a 130 cgc Ar g tgg Tr p at c SIle gga G y gga 210 gcc Al a ggc G y aaa Lys gac Asp ct g Leu 290 gaa G u cgc Ar g ct c Leu gca Al a gct Al a t cc Ser 195 cag G n gaa G u gac Asp t cc Ser gat Asp 275 gcc Al a aag Lys acc Thr at c SIle gcc Al a t cc Ser 180 ttc Phe aaa Lys cgc Ar g ct a Leu ccc Pr o 260 ggc G y t ac Tyr cgc Ar g aaa Lys cgc Ar g ggc G y 165 cgc Ar g gac Asp aac Asn at g Met gaa G u 245 acc Thr ct c Leu at g Met gaa G u 499 547 595 643 691 739 787 835 883 931 979 1027 1077 1080 aat gaa ttc Asn G u Phe 315 cct tac tac tac Pro Tyr Tyr Tyr tagtgagttc atggggtcct <210> 498 <211> 319 <212> PRT <213> Corynebacter i um gl utam cum <400> 498 lIet Ser Asn Tyr Ser Thr Asp Asn Pro Phe Asp Pro Thr n Trp Ala 1 5 10 Thr Val Pro G y Phe G u G u Phe Thr Asp lie Thr Tyr His Arg His Page 629 seql i st cor r ect ed. t xt Val Pr o G n Leu Ser Thr SIle G n 145 Sy Thr Val Val Asp 225 Hi s Thr Thr Ar g Leu 305 Thr Val Leu Sy Sy Hi s 115 Ar g Leu Al a Al a Ser 195 G n G u Asp Ser Asp 275 Al a Lys Ar g Asn Hi s Gy G n Ar g Lys Ar g Sy 165 Ar g Asp Asn Met G u 245 Thr Leu let G u Al a Al a Al a 70 Pr o Ar g Asp Val Thr 150 Sy G n Al a Al a G n 230 Lys Sy bet Thr Pr o 310 Asp Phe Ar g Ser SIle Asp G u 135 bet Sy G u Sy Ar g 215 G n G u G n Sy Asp 295 Asn Sy 40 Ar g Ar g G u Ar g Al a 120 Sy Pr o Hi s Al a Tyr 200 G u Met Al a Ar g G n 280 G u Tr p Ile Val Arg Ile Ala Pro His Thr Val Asp Thr Pro Asp Val y 75 Lys Asp y y Trp 90 Sy Arg Ser y Tyr 105 Thr Ala Asp Val Phe 125 G y Arg Leu His lie 140 Lys Val Val I e Ala 155 Ser Leu His Val Val 170 Arg Phe Lys G n Thr 185 Sy Ser Ala Tyr Leu 205 I e Phe Phe Leu G y 220 Sy Al a Val Asn Ile 235 lie n Ala Ala Arg 250 IVet Leu Lys Phe Al a 265 G n Val Phe Ala y 285 Ala Val G u y Lys 300 Asn G u Phe Pro Tyr 315 Phe Asp Arg Leu SIle Phe Tyr Val G u Val Asp 175 Al a Lys Thr Al a SIle 255 Asn Al a Al a Tyr Tyr Leu Cys Al a Asp Val Asn 160 Leu Asp bet Tyr Asp 240 Asn Leu Thr Phe <210> 499 <211> 384 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(361) <223> RXS02908 <400> 499 Page 630 seql i st cor r ect ed. t xt gccaacgagg gttggtttac cacctctgat tcaggtgaac tccacgacgg gattctcacc gtgactggtc gcgtggatac ccgtcattga ttccggtgga ttg aag ttg cac cca 115 Leu Lys Leu His Pro 1 gag gta ctg gaa cgt gcc atc gca gat att aaa ggt gtc acc gcg gcg 163 Su Val Leu Gu Arg Ala lie Ala Asp Ile Lys y Val Thr Al a Al a 15 tgt gtt gtg ggt att ccc gat ccc cga tta ggc caa gca att gtg gcc 211 Cys Val Val Gy Ile Pro Asp Pro Arg Leu Gy n Ala Ile Val Ala 30 gcg tac tcc gga tcg atc agt ccg tct gaa gtt att gaa ggc ctc gac 259 Ala Tyr Ser y Ser I e Ser Pro Ser G u Val Ile G u y Leu Asp 45 gat cta cct cgt tgg cag ctt ccc aaa cgg ctg aag cat ctg gaa tct 307 Asp Leu Pro Arg Trp G n Leu Pro Lys Arg Leu Lys His Leu u Ser 60 ttg ccc agc att ggt cct gga aaa gct gat cga cgt gct atc gcg aag 355 Leu Pro Ser lie y Pro y Lys Ala Asp Arg Arg Ala lie Ala Lys 75 80 ctg ttt tagtcttcat tcttgctggc tgc 384 Leu Phe <210> 500 <211> 87 <212> PRT <213> Corynebacter i um gl utam cum <400> 500 Leu Lys Leu His Pro u Val Leu u Arg Ala lie Ala Asp Ile Lys 1 5 10 Sy Val Thr Ala Ala Cys Val Val Gy Ile Pro Asp Pro Arg Leu y 25 G n Ala Ile Val Ala Al a Tyr Ser y Ser lie Ser Pro Ser G u Val 40 lie G u y Leu Asp Asp Leu Pro Arg Trp G n Leu Pro Lys Arg Leu 55 Lys His Leu G u Ser Leu Pro Ser lie G y Pro G y Lys Ala Asp Arg 70 75 Arg Ala lie Ala Lys Leu Phe <210> 501 <211> 775 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(775) <223> RXS03003 <400> 501 tcgatgatct gggctcccca ggtggtgcgg ctaagcttgg accacaagat tttgatcacc Page 631 seqI i st car r ect ed. t xt caat gat cgc t gcgct gccg cct caggcat aat ct aacgc ccg Pr o gca A a gt t ValI gca A a g aa G31u ac a Thr gca A a at c I Ie gga G31y at c I Ie 1 50 gt t ValI aac As n g ac As p ggc G31y gt t ValI gcc A a act Thr gca A a cgc Ar g ct t Le u gcc A a gag O31u 120 ac c Thr t cg Ser ggc G31y gt g ValI g aa O31u 200 c ac Hi s gt t ValI gct A a gt t ValI ggg G31y gcc A a g ac As p ct t Le u gcg A a tt g Le u at g vb t cca Pr o 1 70 ct c Le u cca Pr o aac As n ct t Le u gcg A a g at As p tt t Ph e at c I Ie 75 gcc A a cca Pr o t ac Tyr cgt Ar g tt t Ph e 155 cac Hi s gca A a ccg Pr o t t g Le u cct Pr a t ct Ser ccg Pr a t cg Ser ag g Ar g t cc Ser tt c Ph e cca Pr a t ct Ser 140 at c I Ie tt g Le u gct A a at c I Ie at c I Ie 220 g at As p c ac Hi s gt c ValI 45 ggt G31y ggc G31y ct c Le u gcg A a ac c Thr 125 g at As p aac As n cgc Ar g g at As p t ca Ser 205 gcc A a tt t Ph e ccg Pr a gcg A a t ac Tyr gcg A a ct c Le u tt g Le u 110 t ac Tyr t cg Ser t ca Ser aag Ly s g aa O31u 1 90 at t I Ie at t I Ie ccg Pr a 15 g at As p ccc Pr a cct Pr a ct t Le u ccc Pr a 95 ggt G31y g aa O31u ct g Le u cca Pr a gt t ValI 1 75 t gc cys tt g Le u c ac Hi s t gg Tr p ggg G31y ag c Ser c aa O31n gag G31u gt c ValI at t I Ie gt c ValI tt t Ph e t cc Ser 1 gt g ValI t ac Tyr g at As p t cg Ser at g vb t 1 g at As p at c I Ie at t I Ie ac c Thr cgg Ar g gt g ValI t cc Ser gct A a aag Ly s 145 aac As n aag Ly s tt g Le u cca Pr a ct g Le u 225 acc t ct cgc Thr Ser Ar g t cg ct c gct Ser Leu A a gt g aat ct t Val Asn Leu cag at c gcg 03 n IlIe A a at c ggc acc Il e 03 y Thr cgc t ac aac Ar g Tyr Asn ggt acc aag G31y Thr Lys 1 00 ggc acc gtt 03 y Thr Val 115 gt c gt g gcc Val Val A a 130 ct c ggc ccg Leu G31y Pr o ccc aca ggc Pr o Thr G31y t gg gcg cag Tr p Ala O31n 1 ggt ct t ggc O3 y Leu 03 y 1 cgt gt c t gc Ar g Val Cys 210 <210O> 502 <21 1> 225 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 502 Page 632 Met Thr Ser Arg Thr Pro Leu 1 5 seql i st cor r ect ed. t xt Val Ser Val Leu Pro Asp Phe Pro Trp Asp SIle SIle Thr Ar g Val Ser Al a Lys 145 Asn Lys Leu Leu Asn I le Gy Tyr Thr Thr 115 Val Gy Thr Al a Leu 195 Ser Ser Leu Pr o bet G u Val Al a G n Lys 165 G u Tr p Al a Val Al a G u 70 Thr Al a SIle Gy SIle 150 Val Asn Asp Lys Gy G u Leu Lys l Ie Pr o Cys 135 Pr o Leu Asn Asp Al a Thr 40 Al a Ar g Leu Al a G u 120 Thr Ser Gy Val G u 200 Lys Pr o Al a Al a Val Leu 105 I le Val Met SIle SIle 185 Asn Al a Asp Phe l Ie Al a Pr o Tyr Ar g Phe 155 Hi s Al a Pr o Ser Pr o Ser Ar g Ser Phe Pr o Ser 140 I le Leu Al a SIle Hi s Val Gy Gy Leu Al a Thr 125 Asp Asn Ar g Asp Ser 205 Pr o Al a Tyr Al a Leu Leu 110 Tyr Ser Ser Lys G u 190 I le Asp Pr o Pr o Leu Pr o Gy G u Leu Pr o Val 175 Cys Leu Pro Arg 210 Val Cys Asp G y His Thr Asn Leu Ile Ala lie His Ser 220 Leu 225 <210> 503 <211> 390 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(367) <223> RXS03026 <400> 503 gttggcggcg cagtatcgtg aggtgcggga cctcgagcgg ggaatcccaa actagcatcc cgaactagcc ccccaacaac aattagaaat ggaacctaaa atg cct gga aaa att IVet Pro Gy Lys Ile 1 ctc ctt ctc aac ggc cca aac ctg aac atg ctg ggc aaa cgc gag cct Leu Leu Leu Asn y Pro Asn Leu Asn IVet Leu Gy Lys Arg u Pro 15 gac att tac gga cac gac acc ttg gaa gac gtc gtc gcg ctg gca acc Page 633 Asp gct Al a cac Hi s at c SIle ttt Phe Tyr gct Al a ggt Gy tgc Cys at g vbt seql i st cor r ect ed. t xt His Asp Thr Leu G u Asp Val Val Ala Leu Ala Thr aaa cac ggc ctt gag gtt gag gcg ctg cag agc aat Lys His Gy Leu G u Val G u Ala Leu G n Ser Asn 45 cta atc gat gcg ctg cac aac gct cgc ggg acc cac Leu II e Asp Ala Leu His Asn Ala Arg G y Thr His att aac ccc ggc ggc ctg act aca ctt cgg tgg cgc lie Asn Pro Gy y Leu Thr Thr Leu Arg Trp Arg 75 80 tgaaggcgtc tgagcttcct acc <210> 504 <211> 89 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 504 IVet Pro y Lys lie Leu Leu Leu Asn Gy Pro Asn Leu Asn IVet Leu 1 5 10 G y Lys Arg G u Pro Asp I I e Tyr G y His Asp Thr Leu G u Asp Val 25 Val Ala Leu Ala Thr Al a G u Ala Ala Lys His G y Leu G u Val G u 40 Al a Leu G n Ser Asn His n G y G u Leu I e Asp Al a Leu Hi s Asn 55 Ala Arg G y Thr His lie G y Cys Val lie Asn Pro G y G y Leu Thr 70 75 Thr Leu Arg Trp Arg Phe Trp IVet Leu <210> 505 <211> 621 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(598) <223> RXS03074 <400> 505 tttgtgggca atctggtttt ttcgtaattg tgtgggatga atctcttaaa aattcacatt tagcaggaca agcatactgt tttagt t cta tgctgtgggc atg act caa agt gct bet Thr G n Ser Ala 1 cca gaa ttc att gcc acc gca gac ctc gta gac atc atc ggc gac aac Pro u Phe lie Ala Thr Ala Asp Leu Val Asp lie lie y Asp Asn 15 gcg caa tca tgc gac act cag ttt caa aac ctt gga ggt gcc aca gaa Al a n Ser Cys Asp Thr G n Phe G n Asn Leu G y G y Al a Thr G u Page 634 seql i st cor r ect ed. t xt 30 ttc cac gga ata ata acc acc gtg aaa tgc ttc caa gac aac gcc ctc 259 Phe His G y lie lie Thr Thr Val Lys Cys Phe G n Asp Asn Ala Leu 45 ctg aaa tcc atc ctg agc gag gat aat cct ggg gga gtg ctg gtt atc 307 Leu Lys Ser Ile Leu Ser G u Asp Asn Pro Gy y Val Leu Val lie 60 gat ggc gac gca tcc gtg cac acc gcg cta gtt ggc gac atc att gca 355 Asp G y Asp Ala Ser Val His Thr Ala Leu Val G y Asp lie ile Ala 75 80 gga ctt gga aaa gat cat ggt tgg tcc gga gta att gtc aac gga gca 403 G y Leu G y Lys Asp His G y Trp Ser G y Val Ile Val Asn G y Ala 95 100 att cga gac tcc gca gtc atc ggc acc atg acc ttt ggt tgt aaa gcc 451 Ile Arg Asp Ser Ala Val Ile G y Thr Iet Thr Phe y Cys Lys Ala 105 110 115 ctt gga acc aac ccg cgg aaa tcc act aaa act ggt tcc ggc gaa cga 499 Leu y Thr Asn Pro Arg Lys Ser Thr Lys Thr y Ser Gy G u Arg 120 125 130 gac gta gtg gta tcg att ggt ggc att gac ttc att cct ggt cat tac 547 Asp Val Val Val Ser lie G y G y lie Asp Phe lie Pro G y His Tyr 135 140 145 gtc tac gcg gac tct gac gga att atc gtc acc gag gcg cca att aag 595 Val Tyr Ala Asp Ser Asp Gy lie lie Val Thr u Ala Pro lie Lys 150 155 160 165 cag taatttgttt tgacgacgca gta 621 G n <210> 506 <211> 166 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 506 IVet Thr G n Ser Ala Pro G u Phe I e Ala Thr Ala Asp Leu Val Asp 1 5 10 Ile Ile y Asp Asn Al a G n Ser Cys Asp Thr G n Phe G n Asn Leu 25 G y G y Ala Thr G u Phe His G y IIe IIe Thr Thr Val Lys Cys Phe 40 G n Asp Asn Ala Leu Leu Lys Ser Ile Leu Ser G u Asp Asn Pro y 55 Sy Val Leu Val Ile Asp y Asp Ala Ser Val His Thr Ala Leu Val 70 75 G y Asp lie lie Al a G y Leu G y Lys Asp His G y Trp Ser G y Val 90 lie Val Asn y Ala lie Arg Asp Ser Ala Val Ile y Thr IVet Thr 100 105 110 Phe G y Cys Lys Ala Leu G y Thr Asn Pro Arg Lys Ser Thr Lys Thr 115 120 125 Page 635 seqI i st car r ect ed. t xt Gy Ser Gy Ou Arg Asp Val Val Val Ser Ilie y Gy lieAsp Phe 130 135 140 li1e Pro G31y Hi s Ty r Val Ty r Ala Asp Ser Asp y Ilie lieVal Thr 145 150 155 160 O31u Ala Pro I Ie Lys O31n 1 <210O> 507 <21 1> 3075 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (3052) <223> RXCO1434 <400> 507 ggt t t cct gc gcaccgt gat gat t ggt gcg gcgct gt cgc aacact gcca acacgct gcc caacct gat c act ggaaat c cggccat cgc t t cggcgt t c ct g Le u gcc A a ac g Thr ct g Le u t cc Ser ct g Le u ccc Pr o ct g Le u gt g ValI ct t Le u 1 50 t cg Ser ggc G31y ct g Le u cgg Ar g gcc A a gcc A a gcg A a gt g ValI 120 at t I Ie gt g ValI gt t ValI t cc Ser ggt G31y at g vb t gcg A a tt c Ph e gca A a at g vb t g at As p gca A a ccg Pr o tt c Ph e ac c Thr t cc Ser t gg Tr p gct A a gt t ValI ct g Le u c ag O31n 140 t gt Cys gt c ValI tt c Ph e at c I Ie 45 t ct Ser ct g Le u gt g ValI gt c ValI cct Pr o 125 at c I Ie ct a Le u ct t Le u ag g Ar g ct g Le u gag O31u ct g Le u tt g Le u aac As n 110 gcg A a at t I Ie at c I Ie ac c Thr 15 cgg Ar g t cg Ser gga G31y cca Pr o aac As n 95 aat As n cgt Ar g tt c Ph e at g vb t cgc Ar g ct g Le u at t I Ie c aa O31n c ag O31n ac c Thr gt g ValI tt g Le u ct c Le u at t I Ie 1 gt g ValI 1 gcg A a ct c Le u at c I Ie gt c ValI at t I Ie cgt Ar g at c I Ie c ac Hi s ggc O31y 145 ccg Pr o ttg ggt gcg Leu G y A a g aa O31u ac c Thr ggc O31y aac As n tt c Ph e g aa O31u ac c Thr ccg Pr a 130 gt g ValI t ac Tyr aaa Lys5 ct g Le u gcg A a gt g ValI tt c Ph e gt g ValI tt g Le u 115 cat Hi s ggc O31y ct g Le u g aa O31u t cg Ser ccg Pr a gt c ValI t ac Tyr tt c Ph e 1 00 ac c Thr gag O31u ac c Th r cgt Ar g Page 636 seql i st cor r ect ed. t xt gcg ggc att gat Al a G y ie Asp atg cgc cct ctg tgg ggt NIet Arg Pro Leu Trp Gy 170 175 atc gat gcg I le Asp Al a caa G n cag G n gct Al a t at Tyr 230 t cc Ser cag G n ttc Phe ggc Gy ttc Phe 310 cgt Ar g gcc Al a ct g Leu ggt Sy aac Asn 390 tgg Tr p ggg Gy ggt Gy ggt Sy 200 ccg Pr o at c SIle aac Asn ggt Gy acc Thr 280 ttc Phe gct Al a ttt Phe at c l Ie agc Ser 360 agt Ser ct c Leu ttg Leu ct g Leu at g NMbt at c SIle at t SIle ggc Gy gca Al a 250 aag Lys ttc Phe gcc Al a acg Thr gcg Al a 330 gcc Al a ccg Pr o at c SIle ct g Leu tct Ser 410 caa G n gcg Al a at c SIle t at Tyr gt c Val 235 gac Asp ct a Leu ggt Sy aac Asn ct g Leu 315 cgt Ar g acc Thr gag G u acc Thr tt g Leu 395 gcg Al a gcc Al a at g NMbt acc Thr cag G n 220 acc Thr ggc G y acc Thr gt c Val gcc Al a 300 at t SIle gaa G u aag Lys cgt Ar g ggc G y 380 ggt G y gcg Al a gt c Val gcg Al a act Thr 205 cag G n ttg Leu gat Asp ttc Phe cct Pr o 285 gcc Al a cct Pr o gag G u gt c Val gt g Val 365 gcg Al a at g Met gtt Val gt g Val at c l Ie 190 cgc Ar g cac Hi s ct c Leu gat Asp at c SIle 270 at t SIle aac Asn t ac Tyr gt c Val gt g Val 350 gt g Val gt c Val cgc Ar g ggt G y ggc 30y 430 at c SIle at t SIle tgg Tr p acc Thr agg Ar g 255 gca Al a gcc Al a at c SIle gct Al a tgg Tr p 335 ctt Leu gt g Val at c SIle tct Ser gca Al a 415 gat Asp gt g Val gcg Al a at g Met gcg Al a 240 gca Al a ct g Leu aat Asn ctt Leu ttg Leu 320 acc Thr t cc Ser ctt Leu ggc G y ttg Leu 400 gca Al a ttc Phe t ac Tyr tcg Ser ttg Leu 225 at t l Ie gt a Val at c SIle ggc Sy ggt Sy 305 gt g Val cca Pr o ct g Leu ctt Leu gcg Al a 385 gct Al a gca Al a ttg Leu gt g Val at t SIle 210 ct g Leu at g Met gt c Val ccc Pr o ctt Leu 290 tgg Tr p ct g Leu acc Thr ttg Leu ggt G y 370 t at Tyr aaa Lys gca Al a ct g Leu cgt ttg aag Arg Leu Lys 180 gca at c t cc Al a i e Ser 195 gca gac gat Al a Asp Asp caa gtt cct G n Val Pro ccg cga ct g Pro Arg Leu 245 tct gac ctt Ser Asp Leu 260 atc gtg gt g Ile Val Val 275 ttt gcc tac Phe Al a Tyr act ct g agc Thr Leu Ser ct a cat ct g Leu His Leu 325 ttc atc atc Phe lie lle 340 gca ccg ct g Ala Pro Leu 355 gcg gcc aac Al a Al a Asn ctg ttg cgc Leu Leu Arg acc t cc ct g Thr Ser Leu 405 tgg gcg ttg Trp Ala Leu 420 ggc act ct a G y Thr Leu 435 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 Page 637 seql i st cor r ect ed. t xt c ctg gct gtg ttg gg agc tcc gta ggc tac Ser Ser Val y Tyr ttg ttg aa t gtc ttc ttc Val Phe Phe Leu Leu Asn Leu Al a Val Leu G y 450 440 445 at c SIle caa G n 470 at t SIle cag G n cct Pr o gtt Val cac Hi s 550 acc Thr cca Pr o gt g Val gcc Al a gcc Al a 630 gcc Al a at c SIle tgc Cys gt c Val ttg Leu ccg Pr o ttc Phe ccg Pr o 520 ggc Gy ggc Gy aag Lys gcg Al a cgc Ar g 600 aac Asn tgg Tr p ccc Pr o gag G u at t SIle 680 ggc Gy cag G n acc Thr 490 acc Thr ccg Pr o cca Pr o caa G n gt c Val 570 ct g Leu acc Thr cac Hi s cct Pr o gcc Al a 650 cac Hi s at c SIle at c SIle gca Al a 475 aag Lys cag G n at g Met gt c Val ggc Gy 555 gcg Al a tct Ser aag Lys t cc Ser ggc Gy 635 gcc Al a gag G u aac Asn gt g Val 460 ct g Leu at c SIle ct g Leu t cc Ser ggc Gy 540 gcg Al a ct g Leu tcg Ser aag Lys gag G u 620 t cc Ser gcg Al a at g Met acc Thr ttg Leu acc Thr tct Ser gt c Val gcc Al a 525 gac Asp cgt Ar g at c SIle gca Al a ct g Leu 605 gcg Al a agc Ser ttc Phe ggt Gy gac Asp 685 t ca Ser cgc Ar g ttg Leu gcg Al a 510 ggt Gy ggt Gy ttc Phe ttt Phe gcc Al a 590 gcc Al a t ac Tyr ttg Leu gcg Al a at c SIle 670 ggc Gy cgt Ar g at c SIle gat Asp 495 cca Pr o at t SIle cgt Ar g tgg Tr p gt g Val 575 gca Al a agc Ser cgc Ar g agc Ser ct c Leu 655 ccg Pr o cat Hi s cgc Ar g tct Ser cca Pr o 480 gt c Val agc Ser gt c Val ttc Phe cag G n 560 gat Asp gcg Al a ttg Leu aac Asn gcc Al a 640 gcg Al a gcc Al a gcc Al a gac Asp ggt Gy 465 ggt G y ggc G y gag G u cgc Ar g cgt Ar g 545 gcc Al a act Thr ggc Sy ggc Sy ggt Sy 625 gt c Val gaa G u ggc G y gt c Val gcc Al a 705 ttg Leu ttg Leu gaa G u ttc Phe gga G y 530 ct g Leu cgc Ar g t cc Ser at c SIle agc Ser 610 tgc Cys gcg Al a ct a Leu ttg Leu ct c Leu 690 aag Lys ccg Pr o agc Ser gt c Val gca Al a 515 cct Pr o ct c Leu gag G u ggc Sy gcc Al a 595 ttg Leu ct c Leu gaa G u act Thr gac Asp 675 gcc Al a t cc Ser gag G u cgg Ar g t cc Ser 500 gca Al a cgc Ar g gcc Al a at c SIe aac Asn 580 t ac Tyr gcg Al a at t SIe t cc Ser gaa G u 660 aac Asn ttg Leu ct g Leu 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 gcg att ttg ccc gat gcc tca gag ctc Ala lie Leu Pro Asp Ala Ser u Leu Page 638 seqI i st car r ect ed.I t cg gcc gcc gag at g ct t at c gac gcg acc ct c gct Ser Ala Ala Ou I'bt Leu Ilie Asp Ala Thr Leu Ala ccc agc gac gt c Pr o Ser Asp Val 710 715 aag Ly s g at As p gag O31u gcg Al a gca Al a 790 ggt G y t cc Ser gt g ValI g ac As p ac c Thr 870 ac g Thr g ac As p t ca Ser t cc Ser ggt G31y 950 c aa O31n at g gca Al a cca Pr o 760 cgt Ar g at c I Ie gcg Al a tt g Le u cca Pr o 840 gca Al a t gg Tr p gcc Al a aac As n c ac Hi s 920 cgc Ar g gt g ValI t ca Ser act Thr 730 ct t Le u c ac Hi s ggt G31y gct Al a ct c Le u 810 t ct Ser gt c ValI ac a Thr t cc Ser ct c Le u 890 ac c Thr ct g Le u ct c Le u t cc Ser ac c Thr 970 gaa (31u gcc Al a ct t Le u t t c Ph e gt g ValI 795 acc Thr gt t ValI t ac Tyr g at As p acc Thr 875 gac As p t cg Ser t cg Ser ag c Ser gt g ValI 955 aat As n gcc Al a at g vb t gt g ValI ggt G31y 780 gt g ValI ag c Ser g aa (31u ct g Le u gt c ValI 860 ggc G31y cgc Ar g ac c Thr g aa (31u c ac Hi s 940 gt g ValI cca Pr o ggg (31y at g vb t 750 aag Ly s t cc Ser at c I Ie tt c Ph e ac c Thr 830 ct g Le u g ac As p g ac As p at c I Ie aag Ly s 910 at c I Ie at c I Ie ct g Le u at g vb t ct a Le u 735 cgc Ar g aag Ly s g ac As p tt g Le u ggc G y 815 ac c Thr g at As p gt c ValI ggc Le u 895 at c I Ie gag 0 u cgc Ar g gt c ValI c ag O n 975 720 gct Al a ac c Thr g aa O31u t ac Tyr gt t ValI 800 ag c Ser t ct Ser c aa O31n ac c Thr ct c Le u 880 ac c Thr t ac Tyr at c I Ie g at As p g at As p 960 at c I Ie ac a Thr t gc cys aag Ly s ac c Thr 785 t cc Ser ag c Ser gca Al a gcc Al a g ac As p 865 ct a Le u ac c Thr gca Al a ggc G31y t cc Ser 945 gag O31u g aa (31u gga G31y ac a Thr 770 gt c ValI ct g Le u ac c Thr ac a Thr cgc Ar g 850 ggc G31y gt t ValI ggc Ph e ac c Thr 930 t cc Ser gt t ValI aat As n 740 tt c Ph e aag Ly s ggc G31y gcc Al a g aa G u 820 g aa O31u t gg Tr p ac c Thr ct g Le u ggc 900 g ac As p g at As p ct t Le u t cc Ser 725 ccc Pr o ac c Thr cct Pr o at g vb t gcc Al a 805 c aa G n cct Pr a g at As p t cc Ser t cc Ser 885 t cc Ser gcc Al a t at Tyr ccg Pr a t ca Ser 965 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 gct gaa gt a caa ct t Ala Ou Val On Leu 980 Page 639 seql i st cor r ect ed. t xt gtt ggt tgg taaattacgc gtttgtgatt gac Val Gy Trp <210> 508 <211> 984 <212> PRT <213> Corynebacter i um gl utam cum 3075 <400> 508 Val Leu Gy Ala Val Leu Thr Ser 1 Al a Leu SIle Val I le Ar g SIle Hi s Gy 145 Pr o Asp Tyr Ser Leu 225 I le Val SIle Gy Lys Leu Al a Val Phe Val Leu 115 Hi s Gy Leu Ar g Al a 195 Al a G n Pr o Ser SIle 275 Phe 5 Asp Val Leu Mbet Sy Lys Val G n Thr Ar g 165 Lys Ser Asp Pr o Leu 245 Leu Val Tyr Al a Thr Leu Ser 70 Leu Pr o Leu Val Leu 150 Al a G n G n Al a Ty r 230 Ser G n Phe Gy Asp Leu Thr Thr Phe Gy Gy Gy 135 Gy Gy Phe Phe Al a 215 Gy Ar g Leu Phe G n 295 Sy Leu 40 Ar g Al a Al a Al a Val 120 I le Val SIle Sy Sy 200 Pr o SIle Asn Sy Thr 280 Phe Leu Val 10 G y Ser G y G y vbet vbet Phe Al a Leu Phe 90 Trp Al a 105 Tyr Mbet Phe Asp Val Ala Asp Ibet 170 G y let 185 Tyr Ile Phe Il e I e G y Al a Al a 250 Ser Lys 265 Al a Phe Asp Al a SIle Sy Val Leu Tyr 75 Met Pr o Val Pr o G n 155 Ar g Al a SIle Tyr Val 235 Asp Leu Sy Val Phe SIle Ser Leu Val Val Pr o 125 I le Leu Leu Al a Thr 205 G n Leu Asp Phe Pr o 285 Thr Ar g Ser Sy Pr o Asn Asn Ar g Phe Met Sy 175 l e I e Tr p Thr Ar g 255 Al a Al a Ar g Leu SIe G n G n Thr Val Leu Leu SIe 160 I e Val Al a bet Al a 240 Al a Leu Asn Asn Ala Ala Asn Ile Leu 300 Page 640 Gy 305 Val Pr o Leu Leu Al a 385 Al a Al a Leu Leu Gy 465 Gy Gy G u Ar g Ar g 545 Al a Thr Gy Gy Gy 625 Val G u Trp Thr Leu Ser Phe Ser Al 310 seql i st cor r ect ed. t xt a Phe Thr Leu lie Pro 315 Tyr Al a Leu 320 Leu Phe Al a 355 Al a Leu Thr Tr p Gy 435 Val Pr o Ser Val Al a 515 Pr o Leu G u Gy Al a 595 Leu Leu G u Thr Leu 325 I le Leu Asn Ar g Leu 405 Leu Leu Phe Val Phe 485 G u Thr Leu Asp Al a 565 Al a G u Val Val Gy 645 Thr Ar g Al a Leu Gy Asn 390 Tr p Gy Ser SIle G n 470 I le G n Pr o Val Hi s 550 Thr Pr o Val Al a Al a 630 Al a SIle Val Gy Ser Phe 375 Lys Al a Tr p Ser Phe 455 Asn Ar g Asp Val Pr o 535 Sy Gy Phe G n Pr o 615 Asp Asp Gy Tyr Thr 345 Ser Phe Gy Gy SIle 425 Gy Thr Gy Asn Ser 505 Pr o Al a Val G u Pr o 585 Ar g SIle Val Ar g Al a 665 Al a 330 Al a Pr o SIle Leu Ser 410 G n Tyr Gy G n Thr 490 Thr Pr o Pr o G n Val 570 Leu Thr Hi s Pr o Al a 650 Ar g Thr G u Thr Leu 395 Al a Al a Leu SIle Al a 475 Lys G n Met Val Sy 555 Al a Ser Lys Ser Sy 635 Al a G u Lys Ar g Sy 380 Sy Al a Val Leu Val 460 Leu SIle Leu Ser Sy 540 Al a Leu Ser Lys G u 620 Ser Al a G u Val Val 365 Al a bet Val Val Asn 445 Leu Thr Ser Val Al a 525 Asp Ar g SIle Al a Leu 605 Al a Ser Phe Val Val 350 Val Val Ar g Sy Sy 430 Leu Ser Ar g Leu Al a 510 Sy Sy Phe Phe Al a 590 Al a Tyr Leu Al a Tr p 335 Leu Val SIle Ser Al a 415 Asp Al a Ar g SIle Asp 495 Pr o SIle Ar g Tr p Val 575 Al a Ser Ar g Ser Leu 655 Thr Ser Leu Sy Leu 400 Al a Phe Val Ser Pr o 480 Val Ser Val Phe G n 560 Asp Al a Leu Asn Al a 640 Al a His u M\t G y IIe Pro Ala 670 Page 641 seqi i st car r ect ed. t xt Gy Leu Asp Asn Lys Cys Arg lieArg lieAsn Thr 675 680 ValI A a 705 A a Thr CYS Lys Thr 785 Ser Ser A a A a As p 865 Le u Thr A a G31y Ser 945 O31u A a A a Ser Ser As p Le u 755 Pr o Ly s ValI As n Pr o 835 Thr As n As p Thr As n 915 ValI Ly s Ar g ValI Pr o A a ValI 725 Pr o Thr Pr a vb t A a 805 O31n Pr a As p Ser Ser 885 Ser A a Tyr Pr a Ser 965 Le u A a Ser 71 0 Ly s As p O31u A a A a 790 (31y Ser ValI As p Thr 870 Thr As p Ser Ser O31y 950 O31n ValI I Ie 695 A a A a Tyr O31u Thr 775 A a Thr Pr a O31y O31y 855 A a Pr a Ser Pr a G31y 935 O31n Thr G31y Le u A a vb t A a Pr a 760 Ar g I Ie A a Le u Pr a 840 A a Tr p A a As n Hi s 920 Ar g ValI Ser Tr p Pr a O31u ValI Ser 745 Thr As p A a Ph e A a 825 Pr a G31y Thr Ar g ValI 905 Ser Ser O31u As p A a Le u 715 O31u A a Le u Ph e ValI 795 Thr ValI Tyr As p Thr 875 As p Ser Ser Ser ValI 955 As n As p 685 O31u As p O31n A a ValI 765 A a I Ie Ph e A a As p 845 Thr O31y ValI ValI O31y 925 Ser I Ie O31n 03 y Hi s A a Le u A a O31y vb t 750 Ly s Ser I Ie Ph e Thr 830 Le u As p As p I Ie Ly s 910 le le Le u vb t Ar g Thr Le u 735 Ar g Ly s As p Le u G y 815 Thr As p ValI O3y Le u 895 le Ou Ar g ValI O3n 975 <210O> 509 <21 1> 930 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> Page 642 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(907) <223> RXC02080 <400> 509 cgtaaattcg aagcgagctt ctaattctag caagcttggt tttgtcctgt tgcttattgt gcaggaattc ggaggcggac gat ggagt at cct gccaaaa tta Leu gga Gy at t SIle acc Thr cgt Ar g t ca Ser gca Al a ct g Leu act Thr cga Ar g 150 t at Tyr ctt Leu aac Asn at c SIle at t SIle at c SIle gt g Val ggc Gy ct a Leu ct c Leu gtt Val gtt Val 120 ggt Sy acc Thr ggg Gy at c SIle aca Thr 200 t cc Ser gaa G u gt a Val ct g Leu gtt Val gcc Al a ggg G y aca Thr gca Al a at c SIle at c SIle ttc Phe 170 t ca Ser gcg Al a gag G u tta Leu at t SIle ggc Gy cct Pr o cat Hi s 75 at c SIle gca Al a ggt G y tct Ser gt c Val 155 agc Ser acg Thr gca Al a tt g Leu gga Gy gt c Val ctt Leu gga Gy gt a Val acc Thr gt a Val cca Pr o gct Al a 140 t ca Ser gca Al a ct g Leu gtt Val at c SIle 220 gcg Al a gga Gy cca Pr o 45 agt Ser aaa Lys ggc G y at t SIle t ca Ser 125 ggg G y gt a Val gct Al a cag G n aat Asn 205 t cc Ser at c l Ie gca Al a ccg Pr o at t SIle acc Thr gcc Al a ccc Pr o 110 at t SIle ttt Phe tgt Cys caa G n at g Met 190 ct c Leu tgg Tr p ttt Phe 15 gga Gy ttg Leu tcg Ser at c SIle tcg Ser 95 tgg Tr p aag Lys agg Ar g ggt Gy ggc Gy 175 cag G n at c SIle ccg Pr o ggt Gy aca Thr act Thr ggt Gy aga Ar g 80 ct c Leu ct g Leu aag Lys caa G n gcc Al a 160 at t SIle gaa G u gca Al a acc Thr at g Met 1 gca Al a tta Leu gcc Al a gt g Val ttt Phe ttg Leu at t SIle cat Hi s ttg Leu 145 ct g Leu ctt Leu ct c Leu gcc Al a gtt Val 225 tca atc gag tgg Ser Ile Gu Trp ggt ttc ctc gca G y Phe Leu Al a gtg tcg ttt cct Val Ser Phe Pro acc atc gcc aac Thr lie Ala Asn gtt gct tat aga Val Ala Tyr Arg ctg ctg cca gca Leu Leu Pro Ala ctg cat ttc tcc Leu His Phe Ser 100 gga ttc ggc acg G y Phe G y Thr 115 gtt ggc gct cat Val G y Ala His 130 cct ttc ccg agc Pro Phe Pro Ser ttg ctg ggc atg Leu Leu G y IVet 165 ctc atc gca ttg Leu Ile Ala Leu 180 aac gcc atc aaa Asn Ala lie Lys 195 agt gtt ttt ata Ser Val Phe lle 210 gcc tta atc gcg Ala Leu Ile Ala ctt ggc tca gct tta ggt gga tac atc ggc gga cgg tac gcc cgc cga Page 643 Leu G3 y Ser 230 ctt cgc ccc Leu Arg Pro acg gt c at c Thr Val I Ie Le u gt t ValI 250 at g vb t G31y ag a Ar g at c I Ie seqI i st car r ect ed. t xt Tyr lie Oy Oy Arg Tyr Ala Arg Arg 240 245 gca ttt gt g gt c at c gt c gga at c acc Ala Phe Val Val lieVal Oy Ilie Thr 255 260 ggt t aat gcagca gact agt aac ccc G31y <210O> 510 <21 1> 269 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 510 I'bt Ser I Ie O u A a Le u A a ValI Ph e Le u I Ie Hi s Le u 145 Le u Le u Le u A a ValI 225 Ar g I Ie Ph e Ser lie A a Le u Hi s Ph e 115 G31y Ph e Le u I Ie A a 1 95 ValI Le u A a (31y Tr p A a Pr o As n Ar g A a Ser Thr Hi s Ser vb t 1 65 Le u Ly s I Ie A a Ar g 245 Thr Gy le Thr Ar g 70 Ser A a Le u Thr Ar g 150 Tyr Le u As n I Ie Le u 230 Le u Thr Leu G n I I e Val Ser Le u I Ie (31u I Ie As p Le u Ser 135 Thr G31y G31y Le u I Ie 215 G31y Ar g ValI As n Ph e I Ie Hi s G31y Ph e 1 05 li1e G31y Ph e Tyr Thr 1 85 ValI Pr o A a Ser ValI 265 (31u 10 ValI Le u ValI A a G31y 90 Thr A a I Ie I Ie Ph e 1 70 Ser A a (31u Le u ValI 250 Le u I Ie G31y Pr o Hi s li1e A a G31y Ser ValI 1 Ser Thr A a Le u G31y 235 Ph e G31y ValI Le u G31y ValI Thr ValI Pr o A a 140 Ser A a Le u ValI I Ie 220 G31y Ar g A a G31y Pr a Ser Ly s G31y I Ie Ser 125 G31y ValI A a O31n As n 205 Ser Tyr A a I Ie A a Pr a I Ie Thr A a Pr a 110 I Ie Ph e Cys O31n vb t 1 Le u Tr p I Ie Ph e Ph e G31y Le u Ser I Ie Ser Tr p Ly s Ar g G31y G31y 1 O31n I Ie Pr a G31y ValI 255 N/t Thr I Ie (31y Page 644 seql i st cor r ect ed. t xt <210> 511 <211> 669 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(646) <223> RXC02789 <400> 511 ttcttccgta cggctatgct taaaagctaa atttgtcttt ttcttaacac taaacaatgg aaaggtaagc gggtttttct gtgccttgca acacattaat atg aag gt t let Lys Val t cc gcc Ser Al a gat Asp gat Asp aag Lys ggg G y t ac Tyr aat Asn cgt Ar g gac Asp gga Gy gt a Val 150 cca Pr o gga Gy ccc Pr o ggc Gy gcc Al a gac Asp gcc Al a t cc Ser cgc Ar g cgc Ar g 120 agc Ser at c SIle t cc Ser cac Hi s gt g Val cct Pr o gcc Al a gaa G u agt Ser acc Thr acc Thr aaa Lys caa G n gac Asp 170 gat Asp cgc Ar g gt g Val gat Asp gt c Val 75 gt g Val aat Asn acc Thr gcg Al a acc Thr 155 acc Thr gat Asp at c SIle gaa G u at t SIle at t SIle aag Lys gct Al a gt c Val gga Gy 140 tgg Tr p caa G n cca Pr o gga Gy acc Thr 45 gat Asp gtt Val cat Hi s gga G y gt g Val 125 cgt Ar g ttg Leu gat Asp ggc Gy gt g Val gtt Val cgg Ar g ggt G y gca Al a aag Lys 110 gcc Al a aaa Lys gat Asp ttc Phe cca Pr o 15 gca Al a ccg Pr o ttg Leu ct a Leu aag Lys 95 aac Asn acc Thr gtt Val gct Al a gac Asp 175 ggc Gy gcc Al a cgg Ar g gt c Val ccc Pr o 80 gaa G u at t SIle caa G n at t SIle cgc Ar g 160 gag G u 1 cgg Ar g tct Ser gaa G u gcc Al a aca Thr at t SIle ccg Pr o gcc Al a gat Asp 145 acc Thr aag Lys cgc Ar g gac Asp act Thr at c l Ie gat Asp gct Al a gt a Val ttg Leu 130 caa G n cga Ar g gga G y ctt Leu cgc Ar g gga G y gtt Val ct g Leu ttc Phe cgg Ar g 115 cgg Ar g gct Al a gcc Al a aat Asn ggc G y gat Asp ttc Phe gag G u cag G n cgc Ar g 100 ctt Leu gcc Al a gcc Al a ctt Leu ttc Phe 180 tta Leu gcc Al a aaa Lys gaa G u gga G y gtc Val ggc G y t ca Ser gca Al a gaa G u 165 cca Pr o tgaaccaaat ccgaaaccgc cgg Page 645 seqI i st car r ect ed. t xt <210O> 512 <21 1> 182 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 512 I'bt Lys Val Ser Ala Asp Thr Pro Gy Ar g Ser (31u A a Th r I Ie Pr o A a As p 145 Th r Ly s Le u Ar g G31y ValI Le u Ph e Ar g 115 Ar g A a A a As n Le u A a Ly s (31u G31y ValI G31y Ser A a (31u 1 65 Pr o ValI Le u Pr o As n G31y Ar g (31u ValI 135 (31u O31n G31y A a 40 As p A a Ser Ar g Ar g 120 Ser I Ie Ser Th r vb t Le u ValI A a Le u 1 05 Le u G31u Le u Th r As p Ar g ValI As p ValI ValI As n Th r A a Th r 1 Th r As p I Ie G31u I Ie li1e Ly s A a ValI G31y 140 Tr p O31n Pr o G31y Th r As p ValI Hi s G31y ValI 125 Ar g Le u As p G31y ValI ValI Ar g G31y A a Ly s 110 A a Ly s As p Ph e Pr a A a Pr a Le u Le u Ly s As n Th r ValI A a As p 1 <210O> 513 <21 1> 903 <212> DNA <213> Cor ynebact er i urn gi ut anri curn <220> <221 CIDS <222> (880) <223> RXC02295 <400> 513 t tcccat gct aat ccgcgaa aat ggcact t caacagccat at act t taaa caacacaaat gatgcaagac tgataccggg atgtgatagg agcgcaccac at g ggg t tg gaa tt a I'b t G31y Leu O31u Leu 1 gca gct agc ggg tgg ggc atc ctc atc gca ggcgcc gcc gta gccgga AlaAlaSer Oy Trp Oy Ilie Leu Ile Ala 1y AaAa Val Ala (1y 15 t gg at c gac gca gt a at c ggc ggt ggc gga ct c gt c ct c at c ccg ct g Trp Ile Asp AlaVal I Ie (1y (1y (1y (1y Leu Val Leu Ilie Pr o Leu 30 Page 646 at c ct c gcg gt c at g ccg Ilie Leu Ala Val I'bt Pro seqI i st car r ect ed. t xt caa ctc gca cct gtg aca gcg ctg gcc tcc (31n Leu Ala Pro Val Thr Ala Leu Ala Ser 45 aac As n gt c ValI gt g ValI g ac As p ggc G31y gcc A a ct c Le u 1 50 ct c Le u gca A a at t I Ie ct g Le u ct t Le u 230 gt c ValI ct g Le u cgc Ar g gct A a c aa O31n at c I Ie 120 CCC Pr a gca A a at g vb t at g vb t tt c Ph e 200 gt c ValI G31y at g vb t gcc A a aaa Ly s t gc cys at g vb t gt g ValI gga G y t ac Ty r t tc Ph e 1 70 aag Ly s at c I Ie aat As n ac c Th r gt c ValI 250 ac c Th r g ac As p ggt G31y ccg Pr a aaa Ly s aaa Ly s 140 ggc G31y gca A a gt a ValI G31y gca A a 220 ct a Le u ct c Le u ggc (31y aaa Ly s gca A a ct g Le u cca Pr a 125 cgc Ar g at c I Ie ct g Le u aac As n c ac Hi s 205 ggc G31y at t I Ie ac c Th r ac g Th r aaa Ly s ggc G31y at c I Ie 110 aac As n t gg Tr p tt c Ph e ct c Le u ac c Th r 1 90 at g vb t gca A a ag a Ar g t gg Tr p gca A a ct g Le u gcc A a 95 at c I Ie tt c Ph e gcc A a gga G31y t cc Ser 1 75 gca A a t gg Tr p c aa O31n t ac Ty r c aa O31n 255 t cg Ser ct t Le u 80 ct g Le u gt g ValI gga G31y gcc A a CCC Pr a 1 60 c aa O31n ac a Th r t gg Tr p ct c Le u gca A a 240 c aa O31n gcg A a 65 gcg A a gct A a tt g Le u ac c Th r at c I Ie 145 gga G31y aat As n aac As n ac c Th r ggt O31y 225 ct a Le u at c I Ie gca tt c A a Phe ct c t ac Leu Tyr gcg agt A a Ser at g ct g I'b t Leu 115 ggc gaa O31y (1u 130 gt t gca Val Ala acc ggc Thr 03 y t tc ct g Phe Leu ct g ggt Leu 03 y 1 ct a gga Leu 03 y 210 gcc cga Ala Ar g ct a acc Leu Thr caa gga O31n (1y ac c Th r gt t ValI ct c Le u 1 00 gt c ValI ag c Ser gt c ValI at g Nibt t cc Ser 1 gcg A a ct c Le u ac g Th r ct g Le u at g vb t 260 ct g Le u ct g Le u at t I Ie gt t ValI aaa Ly s gga G y tt c Ph e 1 t cc Ser ct a Le u gt g ValI gt g ValI gt t ValI 245 t agat aagt c ggggcaaact ct a <210O> 514 <21 1> 260 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 514 I'b t G31y Leu Ou Leu AiaAiaSer Gy Trp Gy Ilie Leu IleAla(3y 1 5 10 Page 647 seql i st cor r ect ed. t xt Ala Ala Val Ala Oy Trp Ile Asp Ala Val Ile Gy y G y G y Leu Val Thr Al a Al a Al a Leu Thr SIle 145 Gy Asn Asn Thr Gy 225 Leu SIle I e Leu Phe Tyr Ser Leu 115 G u Al a Gy Leu Gy 195 Gy Ar g Thr Gy Leu Ser Leu Leu I le Val Lys Gy Phe 165 Ser Leu Val Val Val 245 SIle Asn Val 70 Val Asp Gy Al a Leu 150 Leu Al a SIle Leu Leu 230 Val Leu Lys Ar g Al a Lys Leu Leu 135 I e I e Al a Val Al a 215 Gy Val Al a 40 Leu Ar g Al a G n SIle 120 Pr o Al a Met Met Phe 200 Val Gy Mbt Val Al a Val Val SIle 105 Val Thr Al a Al a Al a 185 I le Al a Gy Ser bet Al a Lys Cys 90 bet Val Gy Tyr Phe 170 Lys SIle Asn Thr Val 250 Pr o Val Pr o Ser Ar g Phe Tr p Asp 155 Thr Val Gy Val Ar g 235 Tyr G n Thr Asp Gy Pr o Lys Lys 140 Gy Al a Val Gy Al a 220 Leu Leu Leu Gy Lys Al a Leu Pr o 125 Ar g SIle Leu Asn Hi s 205 Gy I e Thr Al a Thr Lys Gy SIle 110 Asn Tr p Phe Leu Thr 190 bet Al a Ar g Tr p Pr o Al a Leu Al a I e Phe Al a Gy Ser 175 Al a Tr p G n Tyr G n 255 <210> 515 <211> 1132 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1132) <223> RXN03063 <400> 515 tgtctacaac ttcgccctca tggacatatt aacttcgttg cctcggagag aatactcat acctagacaa cagtttgtat ctcacctcac aggaggaacc gtg gaa gat ctc tca Val G u Asp Leu Ser 1 tac cgc atc ccg cag tcg cgc acc gtg gcc gag cag gtg cca ggg ccg Tyr Arg lie Pro n Ser Arg Thr Val Ala u G n Val Pro Gy Pro Page 648 c 115 163 seqI i st car r ect ed. t xt 15 aag t cg aaa gcg ct g gat gag cgt cga caa gca gca gt a gca cga gca 211 Lys Ser Lys Ala Leu Asp 31u Ar g Arg O31n Ala Ala Val Ala Ar g Ala 30 ct t gca ccg ggt ct g cct gga t ac gt g gt g gac gca gac ggt ggc at c 259 Leu Ala Pr o G31y Leu Pr o G31y Tyr Val Val Asp Ala Asp G31y 31y Ilie 45 t t ggct gac gcg gac ggc aac cgt t t cat cgac ct ggcc tcc ggcat c 307 Leu A a Asp A a Asp 03 y Asn Ar g Phe Ile Asp Leu A a Ser 03 y Ile 60 gcc gt g acc acg gt c ggc gga t cc aac gcg gcc gt C gcg aaa gcc gt C 355 Ala Val Thr Thr Val G31y G31y Ser Asn Ala Ala Val Ala Lys Ala Val 75 80 ggc gcc gca gct gcc cgct t cacc cac acc tgct t catggt ct ca cct 403 G31y AlaAla Ala AlaAr g Phe Thr Hi s Thr Cys Phe I'b t Val Ser Pr o 95 100 t at gaa act t ac gt ggcc at ggcg gag aga ct caac gcct t gact cca 451 Tyr G31u Thr Tyr Val Ala I'b t Ala G31u Ar g Leu Asn Ala Leu Thr Pr o 105 110 115 ggc gat cac gac aag aag agc gcg ctg ttt aac tct ggc gcc gaa gcc 499 03 y Asp Hi s Asp Lys Lys Ser A a Leu Phe Asn Ser 03 y A a 03 u A a 120 125 130 gt g gaa aac gcc gt C aag gt g gca cgc gcc t ac acc ggc aag ggc gcg 547 Val (31u Asn Ala Val Lys Val Ala Ar g Ala Tyr Thr G31y Lys G31y Ala 135 140 145 gtc gtg gtg ttc gac aac gcg tac cac gga cgg acc aac ctc acc atg 595 Val Val Val Phe Asp Asn Ala Tyr His G31y Ar g Thr Asn Leu Thr I'b t 150 155 160 165 gcg atg acc gcg aag aac cgc cca tac aag tcc gga ttc gga cca cta 643 Ala I'b t Thr Ala Lys Asn Ar g Pr o Tyr Lys Ser G31y Phe G31y Pr o Leu 170 175 180 gcc gca gac gtc tac cgt gca cca atg tct tac cca ctg cgc gac gga 691 Ala Ala Asp Val Tyr Ar g Ala Pr o Nib t Ser Tyr Pr o Leu Ar g Asp G31y 185 190 195 ctg tcc ggc ccg gaa gcc gca gag cgc gcg atc tcc gtg atc gaa tcc 739 Leu Ser G31y Pr o (31u Ala Ala (31u Ar g Ala Ilie Ser Val Ilie (31u Ser 200 205 210 cag gtc gga gcc gaa aac ctc gcc tgc gtg gtc att gaa ccg atc cag 787 31n Val 31y Ala (1u Asn Leu AlaCys Val Val Ile (1u Pr o Ile (1n 215 220 225 ggc gaa ggc gga t tc at c gt c ccc gca cca gga t tc ct c gca gcc at t 835 G31y (1u (1y (1y Phe Ile Val Pr oAla Pr o (1y Phe Leu Ala Aa Ilie 230 235 240 245 t cc acc tgg tgc cgc gag aac gacgt ggt gt t cat cgcc gat gaa at c 883 Ser Thr Tr p Cys Ar g O31u Asn Asp Val Val Phe Ilie Ala Asp O31u Ilie 250 255 260 caa tct ggc ttc ctg cgc acc ggc gac tgg ttc gcc agc gac gca gaa 931 O31n Ser G31y Phe Leu Ar g Thr G31y Asp Tr p Phe Ala Ser Asp Ala 031u 265 270 275 ggt gt g at c ccc gac gt c at c acc acc gca aaa ggc at c gcc ggc ggc 979 31y Val Ile Pr oAsp Val Ile Thr Thr AlaLys (1ylIle Ala (1y (1y Page 649 atg cca IVbt Pro 295 ggc ccc G y Pro 310 gcc gcg Al a Al a acc cgc Thr Arg seql i st cor r ect ed. t xt 280 285 290 cta tcc gca gtg acc ggc cgc gca gaa atc atg gac gca ccc Leu Ser Ala Val Thr Gy Arg Ala G u lie Iet Asp Ala Pro 300 305 ggc gcg ctc ggc gga acc tac ggc gga aac ccc gtt gct tgc Sy Ala Leu G y y Thr Tyr y G y Asn Pro Val Ala Cys 315 320 325 gca ctt gca gcc att gaa gtg atg gaa caa gcc gac ctt aag Ala Leu Ala Al a IIe G u Val Iet G u G n Ala Asp Leu Lys 330 335 340 gcg Al a 1027 1075 1123 1132 <210> 516 <211> 344 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 516 Val G u Asp Leu 1 G n Val Pro G y Ala Val Ala Arg Al a Asp G y G y Leu Al a Ser G y Val Ala Lys Ala Phe IVet Val Ser 100 Asn Ala Leu Thr 115 Ser 0 y Al a 0 u 130 Thr 0 y Lys 0 y 145 Thr Asn Leu Thr G y Phe G y Pr o 180 Pro Leu Arg Asp 195 Ser Val Ile u 210 Ser 5 Pr o Al a SIle SIle Val Pr o Pr o Al a Al a bet 165 Leu Gy Ser Tyr Arg Ile Pro Lys Leu Leu Al a 70 Gy Ty r Gy Val Val 150 Al a Al a Leu G n Ser Al a Al a 55 Val Al a G u Asp G u 135 Val bet Al a Ser Val 215 Al a Gy Al a Thr Al a Tyr 105 Asp Al a Phe Al a Val 185 Pr o Al a Ser Asp Pr o Gy Gy Ar g Al a Lys Lys Asn 155 Asn Ar g Al a Asn Ar g G u Gy Asn Gy Phe Met Ser Val 140 Al a Ar g Al a Al a Leu 220 Thr Ar g Tyr Ar g Ser Thr Al a Al a 125 Al a Tyr Pr o Pr o G u 205 Al a Al a G n Val SIe Al a Thr Ar g Phe Al a Gy Lys 175 Ser Al a Val lle Gu Pro Ile Gn Gy Gu Gy Gy Phe Ile Val Pro Ala Pro Gy Page 650 225 Ph e I Ie Al a G31y I Ie 305 As n O31n Al a As p As p 275 Al a As p ValI As p I Ie 245 li1e (31u G31y Pr o cys 325 Ly s 230 Ser (31n G31y G31y 310 Al a Thr Thr Ser ValI Pr o 295 Pr o Al a Ar g seqI i st car r ect ed. t xt 235 Tr p Cy s Ar g O31u As n Asp 250 G31y Phe Leu Arg Thr G31y 265 Ilie Pr o Asp Val Ilie Thr 280 285 Leu Ser Al a Val Thr G31y 300 03 y Al a Leu 03 y 03 y Thr 315 Al a Leu Al a Al a Il e 03 u 330 Al a ValI 255 Tr p Al a Al a G31y 335 <210O> 517 <21 1> 1491 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1468) <223> RXN02970 <400> 517 aaccgacaaa acagccgtt c acgt gct aaa gcagct cggc t tat t taaag act tcat aat att t tgggga gt gaact ggt t tgat ct agg gt gaggt gag t ac Tyr aaa Ly s c ac Hi s gcc Al a at g vb t tt a Le u aac As n gag (31u t gg Tr p ggt G31y t cc Ser gag (31u g ac As p aaa Ly s gcg Al a ac g Thr ct t Le u at c I Ie g aa (31u gca Al a g ac As p t ac Tyr t cg Ser cgc Ar g tt c Ph e tt c Ph e aaa Ly s 45 g ac As p gca Al a c aa O31n at c I Ie g ac As p at c I Ie tt c Ph e aac As n gca Al a g aa O31u 15 aac As n ag c Ser g ac As p tt a Le u gcc Al a 95 tt c Ph e g at As p ccc Pr o G31y G31y 80 cgg Ar g tt g Le u 1 gga G31y cgc Ar g aaa Ly s aac As n c ac Hi s tt g Le u gca Al a t ct Ser gcg Al a gt a ValI gcc Al a aac As n ac C Thr gca Al a aag Ly s C aa O31n gt t ValI gca Al a at c I Ie cct Pr a at c I Ie 1 00 at c I Ie ggt G31y gca Al a tt c Ph e gct Al a g ac As p cga Ar g aac As n gt g ValI ccg gcc ttc Pro Al a Ph e aat gat gt g cgc t ct gat gt t gct Asn Asp Val Arg Ser Asp Val Ala 110 Page 651 seqI i st car r ect ed. t xt t cg at g gcc Ser I'bt Al a 120 cgt ggc gaa Ar g 31y (31u t tc tc c Phe Ser 125 gcc Al a cgc Ar g 1 50 t cc Ser ac c Thr t ca Ser c ac Hi s at c I Ie 230 gca Al a ct c Le u ct g Le u ac c Thr gt g ValI 310 ggc G31y gca Al a cga Ar g gcc Al a aaa Ly s at g vb t cca Pr o tt t Ph e 200 g aa (31u ct g Le u t ac Tyr at c I Ie gct Al a 280 gcc Al a ac c Thr ct c Le u ct g Le u ggc G31y 360 gag (31u ct g Le u ct c Le u 1 70 at c I Ie ac c Thr gt c ValI cca Pr o aat As n 250 g ac As p gag (31u ggt G31y t ca Ser t ac Tyr 330 at t I Ie g aa (31u t cc Ser 140 gca Al a ggC G31y c ac Hi s c aa O31n gcg Al a 220 gt g ValI gt g ValI gt c ValI gct Al a aac As n 300 cgc Ar g gga G31y gcg Al a at c I Ie g ac As p 380 at c I Ie t ac Tyr g aa G31u tt c Ph e g aa G31u 205 tt t Ph e gga G31y cgc Ar g at g G31y 285 gca Al a g at As p c ac Hi s g aa G31u g aa G31u 365 cac gt g Hi s Val cgc at g Ar g [Vb t cgc agc Ar g Ser cac cgc Hi s Ar g 1 75 t gg gca Tr p Al a 1 90 gaa gaa (31u (1u gaa ggt (31u (1y t ca t ca Ser Ser gaa ctt 03 u Leu 255 gt c gga Val G31y 270 gac gat As p As p ggt t ac (3 y Ty r acc tt c Thr Phe cca ctt Pro Leu 335 gga gag (31y (1u 350 cct cgc Pr o Ar g Phe Phe Thr ttt ttc acc aac gct Al a t ac Tyr 1 60 cgc Ar g cca Pr o t gc cys gct Al a gga G31y 240 t gc cys tt c Ph e tt c Ph e gcc Al a gga G31y 320 gca Al a at c I Ie ct t Le u cgc Ar g 145 C ac Hi s ct g Le u tt c Ph e g aa G31u G31y 225 at c I Ie aac As n gga G31y C ag O31n cca Pr o 305 t ca Ser gt a ValI at t I Ie cgt Ar g gga (31y 385 130 ct g Le u ggC G31y G31y ct g Le u cgc Ar g 210 at g vb t at c I Ie aag Lys5 cgc Ar g cca Pr a 290 ct c Le u gag O31u gca Al a cca Pr a g aa O31u 370 As n c ac Hi s gca Al a aac As n C ac Hi s 1 gca Al a at c I Ie ct g Le u C ac Hi s ac c Thr 275 g ac As p ggt G31y gca Al a ccc Pr a cgc Ar g 355 ct a Le u ggc G31y ac c Thr ac C Thr ccc Pr a 1 c ac Hi s ct c Le u gca Al a cca Pr a G31y 260 gga G31y at g G31y t ac Tyr gcc Al a 340 gt a ValI gcg Al a ggc G31y gga G31y gga G31y 1 ac c Thr t cc Ser aag gcg Al a cca Pr a 245 at c I Ie aaa at c I Ie at c I Ie t cc Ser 325 aag gct Al a g aa (31u 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 gaa aac gt a gcg at c gct cOu Asn Val Ala lieAla 375 gt g cgg ggc at c Val Argc3y Ilie ttc ttc tgg gca Phe Phe Trp Ala Page 652 seqI i st car r ect ed. t xt gt g gag t t c aat gca gac gcc act gcc at g gct gcc ggt Val O31u Phe Asn Al a Asp Al a Th r Al a I'b t Al a Al a G31y 390 395 400 ttc aag gaa cgc ggc gtg tgg ccg atg atc tcc ggc aac Phe Lys 31u Ar g 1y Val Trp Pro 'b t lle Ser 31y Asn 410 415 at c gcg ccg ccg ct g acc acc act gat gac gaa t tg gt a Ilie Al a Pr o Pr o Leu Thr Thr Thr Asp Asp O31u Leu Val 425 430 gac gcg gt g gaa gct gca gcc caa gct gt c gag ct g acc Asp Al a Val O31u Al a Al a Al a O31n Al a Val O31u Leu Thr 440 445 450 gcg t tg t tc t aagtt t tct agataacaag gcc Al a Leu Phe 455 <210> 518 <211> 456 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn gct gca gaa Al a Al a 03 405 cga t tc cac Ar g Phe Hi s 420 gca ct g ct g Al a Leu Leu 435 t tc gct ggg Phe Al a 03 y 1315 1363 1411 1459 1491 <400> 518 Leu Al a Leu G31y Ar g As n Hi s Le u Al a Ph e Ar g 145 Hi s Le u Ph e ValI Hi s Tr p Ph e As n As n Lys5 115 As n Hi s Al a As n Hi s 1 95 Lys 03 y 03 n Al a Val Phe Al a Al a I I e As p Pr o Ar g 1 1 e As n 1 00 I e Val G31YG(1Y Thr 03 y Thr 03 y 1 65 Pr o Thr 1 80 Hi s Ser Lys5 Hi s Al a vb t 70 Le u Pr a Ser Al a Ar g 150 Ser Thr Ser O31u Ser O31u G31y ValI Al a Nibt As p 135 As n Al a As p Ph e Tyr Thr Asn Phe O31u Tr p 40 G31y Ser O31u Ph e Al a 120 Al a Lys5 vb t Pr a Ph e 200 O31u Ser Ser O31n Al a O31y 1 Ar g I Ie I Ie vb t As p 1 Al a As p 10 Lys5 Al a Thr Le u I Ie 90 As n O31y O31u Le u Le u 1 70 li1e Thr G31y Ar g O31n Le u ValI 75 O31n As p O31u His Ser 1 55 Thr Tyr Thr O31u Al a As p Tyr Ser Ar g ValI Ph e Ser 140 Al a O31y Hi s O31n Ph e Ph e Ly s As p Al a O31n Ar g Ser 125 li1e Tyr O31u Ph e O31u 205 O31u As n Ser As p Le u Al a As p ValI vb t Ser Ar g 1 Al a (31u Ph e As p Pr a O31y O31y Ar g ValI Ph e Al a Tyr 1 Ar g Pr a Cys O31u Ar g Al a Leu Lys Hi s Leu O31u Asp Val HIe Al a Phe 0 u 0 y Al a Page 653 seql i st cor r ect ed. t xt 215 220 Val y Ser Ser Gy 225 I le Asn Gy G n Pr o 305 Ser Val SIle Ar g Gy 385 Al a Gy Leu Leu SIle Leu Hi s Thr 275 Asp Gy Al a Pr o Ar g 355 Leu Phe Al a Ar g Al a 435 Phe Al a Pr o 245 I le Lys SIle SIle Ser 325 Lys Al a G u Al a G u 405 Hi s Leu l Ie 230 Al a Leu Leu Thr Val 310 Gy Al a Ar g G u Val 390 Phe SIle Asp Val Leu G u Pro Gy Phe Phe Phe 295 lbt Gy Al a Leu Asn 375 G u Lys Al a Al a Tyr Leu I e Al a 265 Al a Tyr 280 Al a Lys Thr G n Leu Thr Leu G u 345 G y Al a 360 Val Ala Phe Asn G u Arg Pro Pro 425 Val G u 440 Val 235 Gy G u Hi s Val SIle 315 Ser Tyr Leu Al a Asp 395 Val Thr Ar g let Gy 285 Al a Asp Hi s G u G u 365 Val Thr Pr o Thr G n 445 Leu 255 Gy Asp Tyr Phe Leu 335 G u Ar g Gy et SIle 415 Asp Gy 240 Cys Phe Phe Al a Gy 320 Al a SIe Leu SIe Al a 400 Ser G u Al a Al a Al a Ala Val G u Al a y Al a Leu Phe 455 <210> 519 <211> 1330 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1330) <223> FRXA01009 <400> 519 aaccgacaaa acagccgt t c acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag ggt 115 Leu Ala Leu Lys G y 1 tac acc aac ttt gac ggt gaa ttc atc gaa ttc gga tct gtg caa gca 163 Tyr Thr Asn Phe Asp G y u Phe lie G u Phe y Ser Val G n Ala 15 Page 654 seql i st cor r ect ed. t xt aaa Lys cac Hi s gcc Al a at g Met tta Leu ccg Pr o tcg Ser gcc Al a cgc Ar g 150 t cc Ser acc Thr t ca Ser cac Hi s at c l Ie 230 gca Al a ct c Leu ct g Leu gag G u tgg Tr p ggt Sy t cc Ser gag Su ttc Phe gcc Al a 120 gcc Al a aaa Lys at g Met cca Pr o ttt Phe 200 gaa G u ct g Leu t ac Tyr at c SIle gct Al a 280 aaa cgg gca Lys Arg Ala gcg Al a acg Thr ctt Leu at c SIle aat Asn ggc Gy gag G u ct g Leu ct c Leu 170 at c SIle acc Thr gt c Val cca Pr o aat Asn 250 gac Asp gag G u cag G n ct g Leu gt c Val 75 cag G n gat Asp gaa G u cac Hi s t cc Ser 155 acc Thr tac Ty r acc Thr at c SIle gt g Val 235 ggc G y gaa G u cat Hi s gac Asp t ac Tyr tcg Ser cgc Ar g gt g Val ttc Phe t cc Ser 140 gca Al a ggc Gy cac Hi s caa G n gcg Al a 220 gt g Val gt g Val gt c Val gct Al a ttc gac aac Phe Asp Asn aaa atc agc Lys Ile Ser 45 gac ttc gac Asp Phe Asp gca aac tta Al a Asn Leu caa gca gcc G n Ala Al a 95 cgc t ct gat Arg Ser Asp 110 t cc cac gt g Ser Hi s Val 125 atc cgc at g lie Arg IVet tac cgc agc Tyr Arg Ser gaa cac cgc G u His Ar g 175 ttc tgg gca Phe Trp Al a 190 gaa gaa gaa Su G u u 205 ttt gaa ggt Phe G u G y gga t ca t ca G y Ser Ser cgc gaa ctt Arg G u Leu 255 at g gt c gga Mvt Val G y 270 ggc gac gat G y Asp Asp 285 gat Asp ccc Pr o ggc G y ggc G y 80 cgg Ar g gtt Val ttt Phe gct Al a t ac Tyr 160 cgc Ar g cca Pr o tgc Cys gct Al a gga G y 240 tgc Cys ttc Phe ttc Phe cgc gcg Arg Ala aaa gt a Lys Val aac gcc Asn Al a cac aac His Asn ttg acc Leu Thr gct gca Al a Al a ttc acc Phe Thr 130 cgc ctg Arg Leu 145 cac ggc Hi s G y ctg ggc Leu G y ttc ctg Phe Leu gaa cgc G u Arg 210 ggc at g G y tt 225 at c at c Ile Ile aac aag Asn Lys gga cgc G y Arg cag cca G n Pro 290 cac gtt His Val tgg gca Trp Al a ttc atc Phe Il e aac cct Asn Pro aac at c Asn Il e 100 aag at c Lys II e 115 aac ggc Asn y cac acc Hi s Thr gca acc Al a Thr aac ccc Asn Pro 180 cac cac Hi s Hi s 195 gca ct c Al a Leu atc gca I e Al a ctg cca Leu Pro cac ggc Hi s G y 260 acc gga Thr G y 275 gac atg Asp I\et ttc Phe gct Al a gac Asp cga Ar g aac Asn gtg Val ggc gay gga Gy gga Gy 165 acc Thr t cc Ser aag Lys gcg Al a cca Pr o 245 at c SIe aaa Lys at c SIe Page 655 ac c Thr gt g ValI 310 ggC G31y gca A a cga Ar g g aa (31u gt g ValI 390 tt c Ph e gcc A a ac c Thr ct c Le u ct g Le u ggC G31y 360 gt a ValI tt c Ph e g aa (31u ggt G31y t ca Ser t ac Tyr 330 at t I Ie g aa (31u at c I Ie gca A a ggc G31y 410 gt t ValI at c I Ie 315 t cc Ser t ac Tyr ct g Le u gct A a gac As p 395 aac As n 300 cgc Ar g gga G31y gcg A a at c I Ie g ac As p 380 gcc A a seqI i st car r ect ed. t xt gca ggt tac gcc cca ctc Ala G31y Tyr Ala Pr o Leu 305 gat acc t tc gga t ca gag Asp Thr Phe G31y Ser (31u 320 cac cca ctt gca gta gca Hi s Pr o Leu Ala Val Ala 335 gaa gga gag atc att cca 31u G31y 31u Ilie Ilie Pr o 350 gaa cct cgc ct t cgt gaa (31u Pr o Ar g Leu Ar g (31u 365 370 gt g cgg ggc at c gga tt c Val Arg 31y Ilie G31y Phe 385 act gcc at g gct gcc ggt Thr A a I'b t A a A a (3 y 400 ggt G31y gca A a ccc Pr o cgc Ar g 355 ct a Le u tt c Ph e gct A a ggc G31y t ac Ty r gcc A a 340 gt a ValI gcg A a t gg Tr p gca A a at c I Ie t cc Ser 325 aag gct A a g aa (31u gca A a g aa (31u 405 1 027 1 075 1123 1171 1219 1267 1315 1330 <210O> 520 <21 1> 410 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 520 Leu A a Leu Lys G3 y Tyr Thr Asn Phe 1 5 G31y Se r Val O31n Ala Ly s O31u 31u O31u Ar g Ala Hi s Val Phe Hi s Ser Tr p Ser 40 Lys Val Tr p Ala Ala Ala 31u G31y Se r 55 As n Ala Phe I Ie As p 'b t G31y Ser O31n 70 Hi s As n As n Pr o Arg Leu Val O31u Ala Leu Thr As n I Ie Asn Pr o Ala Phe G31y 100 105 Ala Ala Lys I Ie Val Ser I'b t Ala Ar g 115 120 Phe Thr As n 03 y 03 y A a As p A a I I e 130 135 Ar g Leu Hi s Thr G31y Ar g As n Lys I Ie As p (1y 10 Lys Ar g A a 03 n Thr Leu Leu Val lie O31n 90 As n As p G31y 1ul (3 u His Leu Ser Page 656 (3 u Phe I I e 03 u Phe A a As p Ty r Se r Ar g ValI Ph e Se r 140 A a Ph e Lys5 As p A a O31n Ar g Ser 125 li1e Tyr As n Ser As p Le u A a As p ValI vb t Se r seqI i st car r ect ed. t xt 150 Ser Al a I'bt Hi s Le u Ph e (31u G31y 225 li1e As n G31y O31n Pr o 305 Ser ValI I Ie Ar g G31y 385 Al a Al a As n Hi s 1 95 Al a I Ie Le u Hi s Thr 275 As p G31y Al a Pr o Ar g 355 Le u Ph e Al a G31y 1 65 Thr Ser Ly s Al a Pr o 245 li1e Ly s I Ie I Ie Ser 325 Ly s Al a G31u Al a G31u 405 Thr Ser Hi s I Ie 230 Al a Le u Le u Thr ValI 310 G31y Al a Ar g G31u ValI 390 Ph e As p Ph e Le u 215 ValI G31y Ph e Ph e Ph e 295 vb t G31y Al a Le u As n 375 G31u Ly s Pr o Ph e 200 G31u Le u Tyr I Ie Al a 280 Al a Thr Le u Le u G31y 360 ValI Ph e (31u I'b t Leu Thr 1 70 As p I I e Tyr 1 Al a Thr Thr As p Val I Ie G31u Pr o Val 235 Leu Asn 03 y 250 Al a Asp 03 u 265 Tyr 03 u Hi s Lys 03 y Val O31n Ser Ilie 315 Thr Tyr Ser 330 O3 u Il e Tyr 345 Al a 03 u Leu Al a IlIe Al a Asn Al a Asp 395 Ar g G31y 410 03 y 03 u Hi s Hi s O31n Al a 220 ValI ValI ValI Al a As n 300 Ar g G31y Al a I Ie As p 380 Ph e 031u 205 Ph e G31y Ar g vb t G31y 285 Al a As p Hi s 031u 031u 365 ValI Ar g 1 Al a 031u G31y Ser Le u 255 G31y As p Ty r Ph e Le u 335 031u Ar g (31y Al a Thr Al a I'bt Al a 400 <210O> 521 <21 1> 1998 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1975) <223> RXA01551 <400> 521 cact acgt ag gcgt acccca ccat caccgc gt cgaaaagc gccct ctct t aacccccgca agggggt aac tt t tacgcgc acgcgt gcaa cgcgct agt t t tg aag gca gt c ccc 115 Leu Lys Al a Val Pr o 1 Page 657 seql i st cor r ect ed. t xt g atc gcg ctg att gc acg ggc gcc cga gca Thr Gy Ala Arg Ala cgg gct ga Ar g Al a G t gcg u I e Al a Leu Ile Ala Ala ccg Pr o gca Al a ccc Pr o gat Asp cgt Ar g t ca Ser cgt Ar g tcg Ser ggc Gy 150 cgt Ar g gt g Val aac Asn aaa Lys gaa G u 230 gt g Val at t l Ie gaa G u acg Thr cgc Ar g tcg Ser cca Pr o at c SIle ctt Leu 120 gag G u aga Ar g at g Met tct Ser ccc Pr o 200 aac Asn gt g Val gat Asp cgc Ar g gt c Val acc Thr gac Asp agc Ser acc Thr gcc Al a gac Asp aaa Lys acc Thr ttt Phe 170 gt g Val tct Ser aat Asn aag Lys t cc Ser 250 t cc Ser cgg Ar g caa G n ggt Gy ggc Gy 75 aac Asn cgc Ar g gac Asp ggc Gy caa G n 155 gt a Val gcg Al a gaa G u at t SIle ct g Leu 235 acc Thr ccc Pr o tta Leu aat Asn ct c Leu ccc Pr o ccc Pr o ggc Gy ggc Gy caa G n 140 at g Met gcg Al a cgc Ar g ccg Pr o ggc Gy 220 cag G n ggc G y gtt Val gca Al a gag G u 45 gag G u aac Asn aag Lys gac Asp cgc Ar g 125 aaa Lys gcc Al a ct g Leu ggt Sy at g Met 205 aat Asn tgg Tr p gat Asp cct Pr o ccg Pr o at c SIle gt c Val gaa G u cag G n gtt Val 110 tcg Ser cca Pr o t at Tyr cgc Ar g cgg Ar g 190 at t SI e tct Ser gcc Al a gat Asp at c SIle 270 gcg Al a cac Hi s ccg Pr o ccc Pr o gga G y 95 gcc Al a gca Al a gct Al a gca Al a gaa G u 175 gcc Al a at t SIle gcg Al a acg Thr at t SIle 255 ggc G y aag Lys ccg Pr o gag G u ttc Phe 80 ctt Leu acc Thr at g Met cct Pr o cgt Ar g 160 cac Hi s at t l Ie ggt G y gt c Val cgc Ar g 240 cac Hi s acc Thr gaa G u aaa Lys acc Thr cgc Ar g ccg Pr o t at Tyr cgt Ar g ttg Leu 145 gct Al a gtt Val at t SIle cgc Ar g acc Thr 225 tgg Tr p acc Thr gt c Val gag G u cat Hi s gaa G u at c SIle cgg Ar g cag G n cga Ar g 130 aag Lys ggc Sy gat Asp ccc Pr o aaa Lys 210 tct Ser ggt G y acc Thr ccg Pr o agg Ar g agc Ser at c SIle t ac Tyr ct g Leu ggg Gy 115 ggt Sy gcg Al a gt g Val gcg Al a aac Asn 195 ttt Phe t ca Ser gcc Al a cgc Ar g at c SIle 275 cga gca Arg Ala aat ggt Asn y tac tcc Tyr Ser cgc ct c Arg Leu cgc acc Arg Thr cgc gag Arg G u 100 cgc gag Arg G u caa gct G n Al a ct a cct Leu Pro att act Ile Thr 165 gag ttt G u Phe 180 aac gtc Asn Val ttg acc Leu Thr atc gag Ile u gat acc Asp Thr 245 gaa t gg Su Trp 260 tac caa Tyr G n Page 658 s gcg ctg gaa aaa gta aat ggc gtg Al a Leu G u Lys Val Asn G y Val 280 285 eql i st cor r ect ed. t xt gcc gca gac ctt aac Ala Ala Asp Leu Asn t gg gaa gt a Trp u Val ttc Phe acc Thr 310 cgt Ar g tgt Cys ct g Leu ggc Gy gcc Al a 390 gat Asp at c SIle ttt Phe cac Hi s gcc Al a 470 cgt Ar g gca Al a gcc Al a ct c Leu gat Asp cac Hi s acc Thr gcg Al a gaa G u 360 cgc Ar g ct g Leu caa G n gaa G u act Thr 440 act Thr ct g Leu gac Asp gat Asp agt Ser 520 ct c Leu at c SIle ggc Gy at t SIle 330 cac Hi s ttt Phe gga Gy acc Thr at g lbt 410 aac Asn gga Gy gcc Al a t at Tyr aaa Lys 490 gcc Al a gcg Al a ccc Pr o gag G u 300 ct g Leu t cc Ser gaa G u caa G n ctt Leu 380 ggt Gy gaa G u ct g Leu ct a Leu ggt Gy 460 acc Thr ggc Gy ggt Gy ttt Phe act Thr 540 cag G n ct g Leu cgc Ar g t ca Ser t at Tyr 365 gcc Al a gag G u gga Gy gaa G u gtt Val 445 gca Al a ccg Pr o gt a Val cat Hi s gaa G u 525 gca Al a tgt Cys gct Al a ggc Gy ttc Phe 350 gac Asp gat Asp ct c Leu cct Pr o caa G n 430 acc Thr gct Al a aaa Lys at c SIle ccc Pr o 510 ttc Phe at c l Ie gaa G u t at Tyr gga Gy 335 ct c Leu gt c Val gcc Al a acc Thr gga Gy 415 aag Lys gac Asp cac Hi s gaa G u acc Thr 495 ggc Gy cgt Ar g gct Al a caa G n at c SIle 320 t cc Ser t ac Tyr gca Al a aac Asn caa G n 400 cac Hi s tgg Tr p at c SIle at c SIle cac Hi s 480 t ac Tyr gcg Al a tgg Tr p t at Tyr ggc Sy 305 cca Pr o at t SIle gag G u ttc Phe gac Asp 385 cgc Ar g gt g Val gca Al a gct Al a gcc Al a 465 ctt Leu aag Lys cgt Ar g aat Asn 290 gtg gac Val Asp ct g act Leu Thr atg gcc Vet Al a cat ttc Hi s Phe 355 t cc ct c Ser Leu 370 gcc gcg Al a Al a gcc tgg Al a Tr p cca ct a Pro Leu gcg gac Al a Asp 435 cca ggt Pro G y 450 atg ggt lMVt y ggc ctg G y Leu ct c gct Leu Al a gcc tgg Al a Tr p 515 gat cag Asp n 530 t at Tyr acc Thr ggt G y 340 gac Asp ggt G y caa G n gaa G u aac Asn 420 gca Al a t at Tyr ggc Sy ccc Pr o gcc Al a 500 gac Asp ttt Phe 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 cac gac gaa acc ctg His Asp G u Thr Leu 545 Page 659 ccg gca gag cct gcg aaa acc gca c Pr o Al a O31u Pr o Al a Lys Thr Al a F- 550 555 aag t tc t gc t cc at g cga at t agc c Lys Phe Cys Ser N/t Ar g Ilie Ser C 570 gat caa at c gcg gaa t tg ggg at g c Asp O31n Ilie Al a O31u Leu G31y I'b t F 5855 gct gt t gct t ct agt ggg gca cgg g Al aVal Al aSer Ser 31y Al aArg C 600 605 gaa t tt at t gct ggt ggt gcg gag g 03 u Phe Il e Al a 03 y 03 y Al a 03 u\ 615 620 gat cccagat agc <210> 522 <211> 625 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn I i st car r ect ed. t xt ac t tc t gt t ca at g t gt ggc ccg fis Phe Cys Ser I'b t Cys G31y Pr o 560 565 ag gac at t cgc gat at g t tt ggc ~n As p I Ie Ar g As p Wt Phe G31y 575 580 ct ggg gt t ggg gat t ct t ct agt ~ro G31y Val G31y As p Ser Ser Ser 90 595 iag ggg at g gct gag aaa t cc cgg u G31y I'bt Al a O31u Lys Ser Ar g 610 itt t at cgg cgt tagacagagc (al Tyr Ar g Ar g 625 1 795 1 843 1891 1 939 1 985 1 998 <400> 522 Leu Lys Al a Val Pro Thr Gy Ala le Ou Ly s Thr Ar g Pr o Tyr Ar g Le u 145 Al a ValI I Ie Al a Ar g Ser I Ie Tyr Le u G31y 115 G31y Al a ValI Al a As n 1 95 Al a G31y Ser Le u Thr (31u (31u Al a Pr o Thr 1 65 Ph e ValI Pr o Al a Pr a As p 70 Ar g Ser Ar g Ser G31y 150 Ar g ValI As n Ph e vb t I Ie As p G31y Tr p Le u Al a 135 Ly s (31u Ar g Hi s Ar g Pr a Pr a Ly s Pr a (31u Thr 1 li1e Tr p ValI (31u (31u 1 (31u Al a 10 ValI Thr As p Ser Thr 90 Al a As p Ly s Thr Ph e 1 70 ValI Ar g Ar g O31n G31y G31y As n Ar g As p G31y O31n 1 ValI Al a Al a Le u As n Le u Pr a Pr a G31y G31y O31n 140 vb t Al a Ar g O31u Al a O31u O31u As n Ly s As p Ar g 125 Ly s Al a Le u (31y Al a Al a Hi s Pr a Pr a O31y Al a Al a Al a Al a O31u 1 Al a Ser Ou Pro I'bt Ilie Ilie y 205 Page 660 Arg Lys 210 Phe Leu Thr Lys Ile As 215 seql i st cor r ect ed. t xt n Ala Asn lie y Asn 220 Ser Al a Val Thr 225 Tr p Thr Val Leu Gy 305 Pr o SIle G u Phe Asp 385 Ar g Val Al a Al a Al a 465 Leu Lys Ar g Asn Hi s 545 Ser Ser Al a Ar g SIle 275 Tr p Asp Thr Al a Phe 355 Leu Al a Tr p Leu Asp 435 Gy Gy Leu Al a Tr p 515 G n G u Cys G u Thr 245 Tr p G n Val lbet Ar g 325 Tr p G u Asp Phe Tyr 405 Met Pr o Asp Thr Asn 485 Hi s Asp Al a Leu Pr o 565 G u 230 Val SIle Al a Phe Thr 310 Ar g Cys Leu Gy Al a 390 Asp SI e Phe Hi s Al a 470 Ar g Al a Al a Leu Pr o 550 Lys G u lbet SIle Leu Ar g 295 I le Val Leu Cys Leu 375 G u Val G n Tyr SIle 455 let Asp Al a let Ser 535 Al a Phe Val Asp Ar g G u 280 Asp Hi s Thr Al a G u 360 Ar g Leu G n G u Thr 440 Thr Leu Asp Asp Ser 520 Leu G u Cys Ser Leu Asn 265 Lys Thr Al a Gy Hi s 345 I le Pr o Lys Val Asn 425 Leu Ser Cys Val Val 505 Lys Asp Pr o Ser Lys Ser 250 Ser Val SIle Gy SIle 330 Hi s Phe Gy Thr let 410 Asn Gy Al a Tyr Lys 490 Al a Al a Pr o Al a Leu 235 Thr Pr o Asn SIle Val 315 Val Ar g Al a Ser SIle 395 Val G u Pr o l Ie Val 475 Thr Lys Ar g Asp Lys 555 Tr p Asp Pr o Val 285 G n Leu Ar g Ser Tyr 365 Al a G u Gy G u Val 445 Al a Pr o Val Hi s G u 525 Al a Al a Al a Asp SIle 270 Al a Cys Al a Gy Phe 350 Asp Asp Leu Pr o G n 430 Thr Al a Lys SIle Pr o 510 Phe l Ie Hi s Thr Arg 240 l e His 255 G y Thr Al a Asp G u G n Tyr Ile 320 G y Ser 335 Leu Tyr Val Ala Al a Asn Thr G n 400 G y Hi s 415 Lys Trp Asp I e His Il e G u Hi s 480 Thr Tyr 495 G y Al a Arg Trp Al a Tyr Phe Cys 560 Asp I e 575 Iet Arg Ile Ser n 570 Page 661 seql i st cor r ect ed. t xt Arg Asp Iet Phe Gy Asp On Ile Ala Gu Leu Gy IVet Pro y Val 580 585 590 G y Asp Ser Ser Ser Al a Val Al a Ser Ser y Al a Ar g u G y Met 595 600 605 Ala G u Lys Ser Arg G u Phe Ile Ala y y Ala G u Val Tyr Arg 610 615 620 Ar g 625 <210> 523 <211> 1013 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> <223> RXA01019 <400> 523 act ttg gcg Thr Leu Al a 1 acc gtt gga Thr Val G y caa gct gga G n Al a G y gca t ca ccc Al a Ser Pro ggt agg cat G y Arg Hi s aag gcg att Lys Al a Il e cca gt a gcc Pro Val Al a gag ttt gtc G u Phe Val 115 tac tcc gcg Tyr Ser Al a 130 gtt att gct Val Ile Ala 145 ctg acg ttg Leu Thr Leu t ca Ser 5 ct c Leu tct Ser t cc Ser caa G n cag G n ttg Leu ggc G y ctt Leu act Thr cgt Ar g 165 ctt Leu ggg Gy ct c Leu agg Ar g tt g Leu 70 aac Asn tt g Leu tta Leu gtt Val gca Al a 150 gcc Al a t ca Ser gaa G u aac Asn gct Al a 55 gat Asp ttt Phe gcg Al a gcc Al a ggc G y 135 at t l Ie cga Ar g ttc Phe ttt Phe ggc Gy 40 gtt Val tgg Tr p gct Al a at t l Ie cgt Ar g 120 gga G y ggt G y cca Pr o ccg Pr o gaa G u gat Asp gt c Val t cc Ser gat Asp t cc Ser 105 ggc G y gac Asp caa G n gga Gy tcg Ser at t SIle gca Al a acc Thr ccg Pr o 75 gag G u ccc Pr o aat Asn acc Thr ggt G y 155 acc Thr ctt Leu cgg Ar g gct Al a gac Asp gaa G u gcg Al a aca Thr caa G n agc Ser 140 gga G y ct g Leu cgc Ar g gt a Val gt g Val at g Met caa G n at g Met cac Hi s cgc Ar g 125 ggg G y t cc Ser gac Asp at c SIle ctt Leu ttg Leu at a SIle ggt G y acc Thr 110 ttg Leu gac Asp ttg Leu ggg G y acg Thr cgg Ar g gtt Val ggg G y gca Al a ccc Pr o gag G u t cc Ser cca Pr o cca Pr o gag G u cat Hi s gcg Al a cag G n cgt Ar g gtg Val gag G u ttg Leu gag G u 160 gtg gcc cac gga Val Ala His Gy 175 Page 662 seql i st cor r ect ed. t xt aag Lys cca Pr o gca Al a gcg Al a 225 caa G n at c l Ie gac Asp tct Ser gt c Val 305 ggt Gy aac Asn 195 gtt Val gcc Al a gcc Al a ccc Pr o tgg Tr p 275 acg Thr aaa Lys tcc gca gca ggc Ser Al a Al a G y cca Pr o acc Thr act Thr aag Lys 245 gaa G u tgg Tr p ggc Gy cgc Ar g gag G u cca Pr o gat Asp 230 tct Ser ct c Leu at c SIle gat Asp cac Hi s 310 cac Hi s ggc G y 215 aac Asn ggt G y ctt Leu tta Leu gcc Al a 295 gaa G u ctt Leu 185 cgc Ar g ggc G y gac Asp cgc Ar g gaa G u 265 ggc Gy tct Ser ct c Leu gct cta ctg cag cac Ala Leu Leu G n Hi s 190 ccc ttg gtg gat gca Pro Leu Val Asp Ala 205 atg gtg gca cgc gct bet Val Ala Arg Ala 220 gga ctg att gtg gat Gy Leu lie Val Asp 235 atc gat gtg gat tcc Ile Asp Val Asp Ser 250 gcc gct tcc gta ctc Ala Ala Ser Val Leu 270 ggg gaa gac cac acc G y G u Asp Hi s Thr 285 gga ttc cgc acc atc Sy Phe Arg Thr Ile 300 gtc acc gta gat aag Val Thr Val Asp Lys 315 ttt ggt Phe G y cac tgc His Cys gct gga Al a y ctt aac Leu Asn 240 tgt agc Cys Ser 255 gga aca G y Thr ct g ct c Leu Leu ggc caa G y G n aaa acc Lys Thr ccc gca ttt tcc gat gga tgg aga agc ttc Pro Ala Phe Ser Asp y Trp Arg Ser Phe 325 330 aat <210> 524 <211> 330 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 524 Thr Leu Ala His Ser Leu Ser Phe Pro Asp 1 5 10 Thr Val y Asp Leu G y G u Phe G u Val G n Ala y Ser Ser Leu Asn y Asp Asp 40 Ala Ser Pro Asn Ser Arg Ala Val Val Thr 55 G y Arg His Phe G n Leu Asp Trp Ser Thr 70 Lys Ala lIe Val G n Asn Phe Ala Asp II e 90 Pag t aat gaccaa caccct at gg 1010 1013 Ser Ile Al a Thr Pro G u e 663 Leu Ar g Al a Asp G u Al a Ar g Val Val bet G n Mbt Sy Thr Ar g Val Sy Al a seql i st cor r ect ed. t xt Pro Val Ala Ala Leu Leu Ala lie Ser Ala Pro Thr His Thr Pro Val 110 100 G u Tyr Val 145 Leu Lys Pr o Al a Al a 225 G n SIle Asp Ser Val 305 Pr o Val 115 Al a Al a Leu Gy Asn 195 Val Al a Al a Pr o Tr p 275 Thr Lys Phe Gy Leu Thr Ar g 165 Ser Pr o Thr Thr Lys 245 G u Tr p Gy Ar g Asp 325 Leu Val Al a 150 Al a Al a G u Pr o Asp 230 Ser Leu SIle Asp Hi s 310 Gy Al a Gy 135 l le Ar g Al a Hi s Gy 215 Asn Gy Leu Leu Al a 295 G u Tr p 105 Gy Asp G n Gy Leu 185 Ar g Gy Asp Ar g G u 265 Gy Ser Leu Ser Mbet SIle Leu G n 170 Al a Pr o Met Gy I e 250 Al a Gy Gy Val Phe 330 Asn Thr Gy 155 Thr Leu Leu Val Leu 235 Asp Al a G u Phe Thr 315 Ar g 125 Gy Ser Val G n Asp 205 Ar g Val Asp Val Hi s 285 Thr Asp Leu Asp Leu Al a Hi s 190 Al a Al a Asp Ser Leu 270 Thr SIle Lys G u Ser Pr o Hi s 175 Phe Hi s Al a Leu Cys 255 Sy Leu Sy Lys <210> 525 <211> 706 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(706) <223> RXA01352 <400> 525 gtgcccaatg cattgggctg agattgcgcg ctgttgctgc gcgggaccgt tcgaacctgt ctggttaaca ccagcgaagg aagcgaggat tgattgtccc gtg ttt gaa aat cgt Val Phe G u Asn Arg 1 ttt gac ctg cgt tgt tat gtt gtg act ggt gcg ggc tcg gtg gat gag Phe Asp Leu Arg Cys Tyr Val Val Thr y Ala y Ser Val Asp G u 15 gtt gtg cac act gcg tct gct gcg gct cgt ggt ggc gcg ggt gtg gtg Page 664 seql i st cor r ect ed. t xt Val Val His Thr Ala Ser Ala Ala Ala Arg G y Gy Al 30 a G y Val Val cag gtg cgt tca aag cct att tcg cca G n Val Arg Ser Lys Pro lie Ser Pro 45 tca aag gtt gcg ctt gag gtt gcg cgg Ser Lys Val Ala Leu G u Val Ala Arg 60 ctt atc gac gac cac ctc cac gtt gct Leu Ile Asp Asp His Leu His Val Ala 75 ctc ccg att cac ggt gtg cat ctt ggg Leu Pro I I e His G y Val His Leu G y gag gct cgt gag ttg ttg ggg cct gag Su Ala Arg u Leu Leu y Pro u 105 110 gga acc cta gaa ctt gtg gcg gcg gcg Sy Thr Leu G u Leu Val Ala Ala Al a 120 125 gat tac atc ggt gct ggg ccg ttt cgg Asp Tyr lie Gy Ala Gy Pro Phe Arg 135 140 ggt cgg cca ccg att ggc ctt gcg ggt Gy Arg Pro Pro lie y Leu Ala G y 150 155 tcc aag gtg ccg atc gtt gcg att ggt Ser Lys Val Pro Ile Val Ala Ile y 170 cgc gct ctc agc gca acc ggt gtg gct Arg Ala Leu Ser Ala Thr y Val Al a 185 190 ttt tct gaa tct gat Phe Ser G u Ser Asp 200 <210> 526 <211> 202 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 526 Val Phe G u Asn Arg Phe Asp Leu Arg 1 5 Sy Ser Val Asp G u Val Val His Thr 25 Sy Ala y Val Val G n Val Arg Ser 40 IVet Arg G u Leu Ala Ser Lys Val Ala 55 Pro Thr Thr Arg Val Leu lie Asp Asp 70 gcg Al a agc Ser t cc Ser 80 gat Asp at c SIle gag G u act Thr ccc Pr o 160 gt c Val gtt Val at g Met cca Pr o tta Leu gat Asp at t SIle ct g Leu ccc Pr o 145 cct Pr o acc Thr gcc Al a agg Ar g aca Thr at g Met at g Met ggg Gy t cc Ser 130 acc Thr ttg Leu cct Pr o at g Met gag G u acg Thr cgc Ar g tcg Ser ttg Leu 115 gat Asp aag Lys gt g Val gcc Al a gt g Val 195 ttg Leu agg Ar g gaa G u gt g Val 100 act Thr gt g Val gat Asp gaa G u gat Asp 180 cgg Ar g gca Al a gtg Val gga Gy ctt Leu act Thr ttg Leu t ca Ser ttg Leu 165 gtg Val gct Al a Cys Tyr Val Val Thr Gy Ala 10 Ala Ser Ala Ala Ala Arg y Lys Pro lie Ser Pro G u Ala Leu G u Val Ala Arg Cys Ser His Leu His Val Ala Ser Ser 75 Page 665 seql i st cor r ect ed. t xt Leu Met Arg O u Gy Leu Pro IIe His Gy Val His Leu G y Gn Asp 90 Asp Iet Ser Val Leu G u Ala Arg G u Leu Leu y Pro G u Ala lle 100 105 110 I e y Leu Thr Thr y Thr Leu u Leu Val Ala Ala Ala Asn u 115 120 125 Leu Ser Asp Val Leu Asp Tyr lie Gy Ala Gy Pro Phe Arg Lys Thr 130 135 140 Pro Thr Lys Asp Ser G y Arg Pro Pro lie y Leu Ala y Tyr Pro 145 150 155 160 Pro Leu Val G u Leu Ser Lys Val Pro Ile Val Ala I e y Asp Val 165 170 175 Thr Pro Ala Asp Val Arg Ala Leu Ser Ala Thr y Val Ala y Val 180 185 190 Ala IVet Val Arg Ala Phe Ser u Ser Asp 195 200 <210> 527 <211> 944 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> RXA01381 <400> 527 tcc gca ggc gtt gga acc atc acg gtc atc gat gac gac acc gtc gac 48 Ser Ala y Val y Thr Ile Thr Val Ile Asp Asp Asp Thr Val Asp 1 5 10 att tcc aac att cac cgc caa atc ctc ttc ggc gca agc gat gtc ggt 96 IIe Ser Asn lie His Arg n lie Leu Phe G y Ala Ser Asp Val G y 25 cga ccc aag gtc gag gtt gcc gcc gag cgc ctc aaa gaa ctc caa cca 144 Arg Pro Lys Val G u Val Ala Ala G u Arg Leu Lys G u Leu G n Pro 40 gac atc acc gtc aac gcg ttg cac gaa cgg atc act cca gaa aac gcc 192 Asp Ile Thr Val Asn Ala Leu His G u Arg lie Thr Pro G u Asn Ala 55 t gc gag c g ctc aat tcc gtg gac ctc gtc tta gac ggc tcc gat tct 240 Cys G u Leu Leu Asn Ser Val Asp Leu Val Leu Asp G y Ser Asp Ser 70 75 ttc tcc aca aaa tac tta gtg tct gat gcc gcc gaa atc acc gga act 288 Phe Ser Thr Lys Tyr Leu Val Ser Asp Ala Ala G u Ile Thr Gy Thr 90 ccc ctc atc tgg gca acg gta ctg cgc ttt cac ggc gaa ctg gca ctc 336 Pro Leu lie Trp Ala Thr Val Leu Arg Phe His Gy G u Leu Ala Leu 100 105 110 ttc aac tct ggc ccc gac cac cgc gga gtc ggc ctg cgc gac gtc ttc 384 Phe Asn Ser G y Pro Asp His Arg Gy Val Gy Leu Arg Asp Val Phe 115 120 125 Page 666 seql i st cor r ect ed. t xt ccc gaa caa ccc tcc gcc gat ttc gtc cct gac tgc gcc acc gct ggt 432 Pro G u n Pro Ser Al a Asp Phe Val Pro Asp Cys Ala Thr Al a y 130 135 140 gtt ctt ggc gcc acc aca gcc acc atc ggc gca ctc atg gcc act cac 480 Val Leu Gy Ala Thr Thr Ala Thr Ile y Ala Leu Mbt Ala Thr His 145 150 155 160 gcc atc gga ttt ctc aca gaa atc ggc gac gtc caa cca ggc aca atc 528 Ala lie y Phe Leu Thr u lie y Asp Val n Pro Gy Thr lie 165 170 175 ctc tcc tac gac gca ttc ccc gcc gcc acg cgc agc ttc cgc gtc tcc 576 Leu Ser Tyr Asp Ala Phe Pro Ala Ala Thr Arg Ser Phe Arg Val Ser 180 185 190 gcc gac ccg gcg cgc cca ctg gtc acc cgc ctc cgc gcc tcc tac gag 624 Ala Asp Pro Ala Arg Pro Leu Val Thr Arg Leu Arg Ala Ser Tyr G u 195 200 205 gca gcg cgc acc gat aca act tcg ctt atc gac gcc acc ctc aac ggc 672 Ala Ala Arg Thr Asp Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn G y 210 215 220 tcc ctc acc gcc ctc gat atc cga gag cca cat gaa gtt ctg ctc aaa 720 Ser Leu Thr Ala Leu Asp lie Arg G u Pro His G u Val Leu Leu Lys 225 230 235 240 gac ctc ccc gag ggc gca acg tca ctg aag ctc ccc tta agc cag atc 768 Asp Leu Pro G u Gy Al a Thr Ser Leu Lys Leu Pro Leu Ser G n Ile 245 250 255 acc tcg gac agc gac att tta gag gca ctg tct gga atc gac ggc gac 816 Thr Ser Asp Ser Asp Il e Leu G u Al a Leu Ser G y Ile Asp G y Asp 260 265 270 att ttg gtc tac tgc gct tcg gga atc cgc agt tcc gac ttc atc gac 864 Ile Leu Val Tyr Cys Al a Ser Gy Ile Arg Ser Ser Asp Phe Ile Asp 275 280 285 aac tac tcc cac ctc ggc cac aaa ttt gtg aat ctt ccc ggt ggg gtc 912 Asn Tyr Ser His Leu G y His Lys Phe Val Asn Leu Pro G y G y Val 290 295 300 aac gcg ctg tagctgtcaa tttaagaggc cag 944 Asn Ala Leu 305 <210> 528 <211> 307 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 528 Ser Al a y Val y Thr Ile Thr Val lie Asp Asp Asp Thr Val Asp 1 5 10 IIe Ser Asn lie His Arg n lie Leu Phe G y Ala Ser Asp Val G y 25 Arg Pro Lys Val G u Val Ala Ala u Arg Leu Lys G u Leu G n Pro 40 Asp Ile Thr Val Asn Al a Leu His G u Arg lie Thr Pro G u Asn Ala 55 Page 667 Cys Phe Pr o Phe Pr o Val 145 Al a Leu Al a Al a Ser 225 Asp Thr SIle Asn Asn 305 G u Leu Leu Asn Ser Val seql i st cor r ect ed. t xt Asp Leu Val Leu Asp G y Ser Asp Ser Thr SIle Ser 115 G n Gy Gy Tyr Pr o 195 Ar g Thr Pr o Asp Val 275 Ser Leu Tyr Al a Pr o Ser Thr Leu 165 Al a Ar g Asp Leu Gy 245 Asp Cys Leu Leu Thr Asp Al a Thr 150 Thr Phe Pr o Thr Asp 230 Al a SIle Al a Gy Val Val Hi s Asp 135 Al a G u Pr o Leu Thr 215 I le Thr Leu Ser Hi s 295 Asp Ar g 105 Gy Val l Ie Gy Al a 185 Thr Leu G u Leu Al a 265 I le Phe Al a Hi s Gy Asp Al a 155 Val Ar g Leu Asp Hi s 235 Leu Ser Ser Asn l Ie G u Ar g 125 Al a let Pr o Phe Al a 205 Thr Val Leu SIle Asp 285 Pr o Gy Al a Val Al a Thr Thr 175 Val Tyr Asn Leu G n 255 Gy lie Gy <210> 529 <211> 259 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(259) <223> RXA01360 <400> 529 gtggcaatca acgccgcggt cgtacccaga tcccagtggt cacgcgccat ttgtgacaac gattccgtag aagttctcac cgcaattcag ggaggttaaa atg ctg cat att gct 115 IVet Leu His Ile Ala 1 gat aaa act ttc gat tcc cac ctc atc atg ggc acc ggc gga gcc acc 163 Asp Lys Thr Phe Asp Ser His Leu I I e Vet G y Thr G y G y Ala Thr Page 668 tct cag gcg ttg ctg Ser G n Ala Leu Leu acc acc gtg gcg atg Thr Thr Val Al a Mbet seql i st cor r ect ed. 15 gag gaa tcc ctt gtc gcc agt Gu u uSer Leu Val Ala Ser 30 cgt cga cac caa gca acc acc Arg Arg His G n Ala Thr Thr 45 txt gga act caa ttg G y Thr G n Leu tct agc gga gaa Ser Ser G y G u <210> 530 <211> 53 <212> PRT <213> Corynebacter i um gl ut am cum <400> 530 IVet Leu His Ile Ala Asp Lys Thr Phe Asp Ser His 1 5 10 Thr G y G y Al a Thr Ser G n Al a Leu Leu G u G u 25 Ser y Thr G n Leu Thr Thr Val Ala Met Arg Arg 40 Thr Ser Ser G y G u Leu Ilie Iet y Ser Leu Val Ala His G n Ala Thr <210> 531 <211> 629 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> RXA01361 <400> 531 gcc gac gct gtg atc tct att gat ggc Ala Asp Ala Val Ile Ser lie Asp y 1 5 acg atg aat tcg ggg gtg agg gtt gcg Thr IVet Asn Ser y Val Arg Val Ala 25 gcg gcg ggc ctg ggc gcc gca agc att Ala Ala G y Leu y Ala Ala Ser Ile 40 aac cca ttg cag ttg agg ccg gtg tac Asn Pro Leu G n Leu Arg Pro Val Tyr 55 gtg ccg gag cga ctg cag ccg atg gtc Val Pro G u Arg Leu G n Pro IVet Val 70 gtg gaa gat cgt cag att tat atc att Val u Asp Arg n lle Tyr lie lle gcg atc ggc gcg aca agc cgt gag gat Ala lle Gy Ala Thr Ser Arg G u Asp 100 105 cac gat ccg His Asp Pro 10 tcg aaa agc Ser Lys Ser ccc ggc tgg Pro y Tr p ggc gat at t G y Asp Il e acc aag gtg Thr Lys Val ccg cgt acc Pro Arg Thr 90 cac ccg caa His Pro G n Page 669 tgt Cys gtt Val ttt Phe gt g Val gt g Val gat Asp cct Pr o ttg Leu gtt Val gag G u cgc Ar g cgc Ar g ggc G y cga Ar g 110 acc Thr gtt Val ggc G y gtg Val ggg G y acc Thr acg Thr seql i gca gtg cat gat ttg cta cgc gat gct Ala Val His Asp Leu Leu Arg Asp Ala 115 120 gaa gaa acc gaa ttt atc gaa gtc acc Su G u Thr u Phe Ile u Val Thr 130 135 ccg gat gac ctg ccg tac ctg gga tgg Pro Asp Asp Leu Pro Tyr Leu Gy Trp 145 150 tcc aca gga tat ttc cgc cat gga att Ser Thr Gy Tyr Phe Arg His y lie 165 cgc gct gcc gtt gat atg gca acc aac Arg Ala Ala Val Asp IVet Ala Thr Asn 180 185 gat gtg tgc gat ccg ttt cgc cac caa Asp Val Cys Asp Pro Phe Arg His G n 195 200 tta <210> 532 <211> 202 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 532 Ala Asp Ala Val Ile Ser lie Asp Gy 1 5 Thr lVbt Asn Ser G y Val Arg Val Ala Ala Al a y Leu Gy Ala Ala Ser lie 40 Asn Pro Leu G n Leu Arg Pro Val Tyr 55 Val Pro G u Arg Leu G n Pro lVbt Val 70 Val u Asp Arg n Ile Tyr Ile Ile Ala Ile y Ala Thr Ser Arg u Asp 100 105 Ala Val His Asp Leu Leu Arg Asp Ala 115 120 Su u Thr u Phe Ile u Val Thr 130 135 Pro Asp Asp Leu Pro Tyr Leu y Trp 145 150 Ser Thr G y Tyr Phe Arg His G y IIe 165 st cor r ect ed. t xt atc cgt ttg att ccg ggc att lie Arg Leu Ile Pro G y Ile 125 tgc ggc gcc cgc ccc ggc acc Cys y Ala Arg Pro y Thr 140 gtt gga tcc aat gtg att gcg Val y Ser Asn Val I e Ala 155 160 ttg ctg tca gcc ctt ggt gca Leu Leu Ser Ala Leu G y Al a 170 175 cag cca ctg ttc ccc act ctt G n Pro Leu Phe Pro Thr Leu 190 att taaggatttt tcacaagtga Ile Asp Lys Sy Asp Lys Ar g Pr o Ar g Sy Sy 155 Leu Pr o Ser Tr p SIle Val Thr G n Leu Al a 140 Ser Ser Cys Val Phe Val Val Asp Pr o SIle 125 Ar g Asn Al a Thr Val Sy Val Sy Thr Thr Sy Gy I e Gy 175 Arg Ala Ala Val Asp IVet Ala Thr Asn n Pro Leu Phe Pro Thr Leu Page 670 seql i st cor r ect ed. t xt 180 185 Asp Val Cys Asp Pro Phe Arg His On lie 195 200 <210> 533 <211> 927 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(904) <223> RXA01208 <400> 533 tactaggcac ggggtgccaa ccggatggaa aaattccgga aacctgctct agctcgtact agcgaaggga tggcct t aac ggctgagaaa acacccgttg ttg Leu ttg Leu gcg Al a ttc Phe gcg Al a gct Al a gag G u at t SIle ggt Gy gcg Al a 150 ggt Gy tct Ser aac Asn gcg Al a gct Al a caa G n aca Thr acc Thr gga Gy 120 cgc Ar g caa G n gag G u act Thr gt g Val cct Pr o gcc Al a caa G n tgg Tr p t at Tyr gcc Al a gt a Val ttg Leu ttg Leu 170 ctt Leu gt c Val gcg Al a aat Asn ggc G y 75 gt g Val gcg Al a t ca Ser gac Asp gcc Al a 155 at t SIle gtt Val at g Met at g Met gga G y at g Met tta Leu gag G u gag G u gcg Al a 140 aag Lys gt g Val cga Ar g caa G n gt g Val 45 gt g Val acc Thr gac Asp gga Gy gt c Val 125 acc Thr cgc Ar g tct Ser caa G n ttc Phe gat Asp ct c Leu aag Lys cca Pr o at c SIle 110 gt g Val gat Asp act Thr gcg Al a aac Asn 15 acg Thr act Thr at c SIle gcc Al a gtt Val 95 gt c Val gcg Al a t cc Ser ggt G y gat Asp 175 act Thr gcc Al a cca Pr o aat Asn at t l Ie 80 gct Al a gat Asp ctt Leu gt g Val ggc G y 160 cgg Ar g gt g Val 1 ccc Pr o aat Asn gct Al a gcg Al a gag G u gt g Val aag Lys gcg Al a gaa G u 145 gt c Val gt g Val ctt Leu gt g Val gaa G u gga G y ggt G y ggt G y cag G n ggg G y 130 gt g Val gt g Val acg Thr gtt Val ttg Leu tcg Ser act Thr gca Al a ggg G y cct Pr o 115 ct c Leu gcg Al a gct Al a tgg Tr p cag G n ctt Leu gca Al a cct Pr o cga Ar g ttg Leu 100 gcc Al a ggt G y ttg Leu gt c Val ttg Leu 180 gct aac t cg Al a Asn Ser ttt Phe tgt Cys gcc Al a gaa G u tct Ser aaa Lys tcg Ser gca Al a gcc Al a gag G u tct Ser 165 cgt Ar g tcg ggg gat ccg atg ttg cag ctg gtg att ggc act gga tgc tct ttg Page 671 Ser ggc O31y t cc Ser ggc O31y 230 gcg A a tt g Le u As p ct g Le u 200 c ac Hi s at t I Ie at t I Ie g at As p vb t gct A a gct A a gca A a gcg A a 250 cga Ar g Le u gca A a gt g ValI cag O31n 235 ct t Le u gag O31u O31n t at Tyr t tg Le u 220 aag Ly s t at Tyr gcc A a seql i st car r ect ed. t xt Leu Val I Ie O31y Thr O31y Cys 190 195 ct a ggc gcc acg gt t gac t ca Leu O31y Ala Thr Val As p Ser 205 210 gct gcg cat gcc cat gt g ggt Ala Ala Hi s Ala Hi s Val O31y 225 gca tcg gcg cca ggc agc ttt Ala Ser Ala Pr o O31y Ser Phe 240 gac gt g gat gcc cag gct gt g Asp Val Asp Ala O31n Ala Val 255 t gaaaagt ac gt gact gat t tt t Ser g at As p gct A a gcg A a gcc A a 260 Le u at t I Ie gct A a gt g ValI 245 t cg Ser <210O> 534 <21 1> 268 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 534 ValI Pr o As n A a A a (31u ValI Ly s A a (31u 145 ValI ValI Thr A a As n Se r Leu Val O31r 2C Val Leu LeL O3 u Se r A E O31y Thr Pr c O31y Ala Ar O3 y 03 y LeL 1 OC O31n Pr o AlE 115 O3 y Leu 031 130 Val A a LeL Val A a Val Thr Tr p LeL 1 8C (3 y Cys Ser 1 95 Ph e Cys A a O31u Ser Ly s Ser A a A a O31u Ser 1 65 Ar g Le u Leu Asp Ser Leu Le u A a Ph e A a 70 A a O31u I Ie O31y A a 150 O31y Ser (31y Thr O31y A a O31u O31y Ar g Ar g O31y 135 A a A a O31y A a As n A a 40 A a O31n Thr Thr O31y 120 Ar g O31n O31u As p Le u 200 Ser 25 Thr ValI Tyr Pr o Ly s 1 05 As n O31y Le u As p Pr o 1 85 Thr Le u ValI A a As n O31y ValI A a Ser As p A a 1 li1e Le u Ar g O31n ValI ValI Thr As p O31y ValI 125 Thr Ar g Ser Le u O31n Ph e As p Le u Ly s Pr o I Ie 110 ValI As p Thr A a ValI 1 As n Thr Thr I Ie A a ValI ValI A a Ser O31y As p 1 li1e Ala Ala Tyr Leu (3y Ala Thr 205 Page 672 Val Asp 210 His Val 225 G y Ser G n Al a seql i st cor r ect ed. t xt Ser Asp Ile Ser Ala His Asp Ala Val Leu Ala Ala His Ala 215 220 G y Al a Al a G y Gn I e Al a Al a n Lys Ala Ser Ala Pro 230 235 240 Phe Ala Val Ala Phe Ile Asp Ala Leu Tyr Asp Val Asp Ala 245 250 255 Val Ala Ser Leu Val Asp Val Arg G u Ala 260 265 <210> 535 <211> 1023 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1000) <223> RXA00838 <400> 535 tcgtctaata gtgctgccaa tccaccggcc at t gatgact tgcttacaaa tcttatctgt gctcaggcaa gatagcaggt cctttgtaga gaaggggtag atg aaa att gcg atc 11! IVet Lys Ile Ala lle gtt Val tct Ser ttg Leu gt a Val gt a Val gaa G u aat Asn cgt Ar g gcc Al a gct Al a gca Al a tct Ser at t SIle at g Met ttg Leu at t SIle tgg Tr p 120 gt g Val gca Al a at c l Ie gga G y gca Al a gct Al a ggg G y tct Ser ggt G y t ac Tyr gtt Val acg Thr ct c Leu gtt Val act Thr 75 at a SIle gct Al a gt g Val aag Lys ggt G y at g Met cac Hi s gcg Al a aaa Lys cct Pr o gat Asp gtt Val gga 10y 140 gga G y gtt Val at c SIle 45 agc Ser gca Al a gcg Al a ct a Leu cgt Ar g 125 ggc G y t at Tyr gca Al a aac Asn gt g Val tta Leu aat Asn gca Al a 110 ggg G y cca Pr o gga Sy gga G y gca Al a gaa G u cgg Ar g 80 gt g Val aag Lys ttt Phe t cc Ser 1 gcg Al a cga Ar g aga Ar g ct a Leu tct Ser gt c Val agt Ser gtt Val t ac Tyr 145 ttg Leu aca Thr ggc G y aaa Lys tta Leu gcg Al a at c SIle cat Hi s 130 acg Thr tta Leu tta Leu gaa G u gat Asp gat Asp at t l Ie ggt G y 115 gag G u ttt Phe caa G n gaa G u cgc Ar g gca Al a ct c Leu act Thr 100 gct Al a ggg gty ggt G y gaa G u gcc Al a t ac Tyr gat Asp gct Al a cag G n gat Asp cca Pr o gat Asp tct ggt gaa ctt tct agg caa ttc gca agc act ctt gaa cag gcc ggt Page 673 seql i st cor r ect ed. t xt Ser y G u Leu Ser Arg G n Phe Ala Ser Thr Leu G u 150 155 160 att gac gga gtt ctg cat ccc gat att I e Asp y Val Leu His Pro Asp Ile 170 gcc atg ttc gta gag gtt ttc ggc ggg Ala Met Phe Val G u Val Phe y y 185 190 caa tta ggt acc ttg cgt acg cat ttt G n Leu G y Thr Leu Arg Thr His Phe 200 205 atg gaa gag gtg gct gag gtg gct cgc IVet G u G u Val Ala G u Val Ala Ar g 215 220 agc gat gcg gtg gag cgc acc atg aat Ser Asp Ala Val G u Arg Thr IVet Asn 230 235 aat tcg acg agt tcg atg cag cgt gat Asn Ser Thr Ser Ser Iet G n Arg Asp 250 gag ctt gag gct cag aca ggt gca att G u Leu G u Ala n Thr G y Ala lle 265 270 ggt gtg aaa act ccg ctt cat gac ctt Gy Val Lys Thr Pro Leu His Asp Leu 280 285 aaa gaa gag gaa aat tca ctt tagggat Lys G u G u G u Asn Ser Leu 295 300 <210> 536 <211> 300 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 536 IVet Lys Ile Ala l e Val Gy Ala Gy 1 5 Al a Leu Leu n G u Ser G y Al a Asp 25 Arg Thr Leu G u Ala Leu Lys Ser Lys 40 Arg Gy G u Arg Tyr Val Pro Ile Pro 55 Leu Lys Asp Ala Asp Val Val IVet lie 70 Ser Leu Asp Leu Ala G u Leu Leu y Val Ala Ile Thr G n Asn Ser Ile G u 100 105 Ser Ile y Ala Asp Arg Val Trp Pro gt g Val ggg Gy gct Al a gca Al a gcg Al a 240 gcc Al a cgg Ar g t at Tyr gat Asp gct Al a t cc Ser ggt Gy 225 gat Asp gcg Al a gca Al a gct Al a gt g Val ttc Phe ct g Leu 210 gtt Val cgg Ar g gga Gy gcg Al a ggt G y 290 G n Al a G y 165 tgg gag aaa Tr p G u Lys 180 gtc gaa aag Val G u Lys 195 gaa gcc ttg G u Al a Leu gcg ttg ccg Ala Leu Pro at g cct gag IVet Pro G u 245 gtg gct agt Val Al a Ser 260 cac aaa gtg Hi s Lys Val 275 ctt aag ctg Leu Lys Leu aga at caagat cc at g Ala Val Gy Gy T 10 Ile Thr Met Val Al G y Leu Hi s I I e A Ala Val Ala Ser V Ala Thr Lys Ala Li 75 Gy Ile Pro Ala A 90 Ser Ala Asp Leu Al 1 Sy Val Val Arg G Page 674 1023 yr a sn al eu sn a y Phe Ar g Asp G n Ser Ser Al a Phe Sy Sy Al a G u Ar g Val Lys Phe seql i st cor r ect ed. t xt Val Tyr 145 Leu Asp Al a Ser Gy 225 Asp Al a Al a Al a G u G y Pro Ala Phe G n Tr p Val 195 G u Al a lb t Val Hi s 275 Leu Asp Gy 165 Lys Lys Leu Pr o G u 245 Ser Val Leu Ser 135 Gy Asp bet Leu G u 215 Asp Ser Leu Val G u 295 Val Ser Tyr Lys Leu Val Val 185 Thr Val Val Ser Al a 265 Thr G u Ar g 155 Hi s Val Ar g G u Ar g 235 et Thr Leu Ser Gy Phe Asp Gy Hi s 205 Al a let Ar g Al a Asp 285 Pro Leu Ser Al a SIle Gy 190 Phe Ar g Asn Asp l Ie 270 Leu Ser Leu 175 Leu Ar g Al a Phe Leu 255 Val SIle Thr 160 Val Gy Al a Al a Al a 240 Al a Ar g Tyr <210> 537 <211> 693 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(670) <223> RXA02400 <400> 537 aggtgccccc aacgttgcct gttgactgca aattttccga tttaagtcgc cagattaaag tcgtcaatga aaggacatac acc Thr gcg Al a gag G u acc Thr ttc Phe tct Ser ttg Leu t cc Ser at c I e cct Pr o act Thr acg Thr gca Al a cac Hi s tct Ser act Thr gcc Al a cag G n cag G n act Thr gca Al a gat Asp cca Pr o t ca Ser gcc ctg Al a Leu 15 t cc cca Ser Pro act tct Thr Ser gct cct Ala Pro Page 675 aagaatccat aaactacttc atg tct att tcc cgc 115 IVet Ser Ile Ser Arg 1 tct gca gct ctc gtt 163 Ser Ala Ala Leu Val gtc cag cgc acc aat 211 Val G n Arg Thr Asn gcg agc agc acc tca 259 Ala Ser Ser Thr Ser gtg gaa gag gac gta 307 Val G u G u Asp Val gag atc gtt gtt tca cca G u Ile Val Val Ser Pro 75 ttc gaa atc tct gga ct t Phe G u I e Ser G y Leu tgc gat tcc gta gcg aac Cys Asp Ser Val Ala Asn 105 gaa atg gct gat ttc acg G u Iet Al a Asp Phe Thr 120 ggc gcg act gta gag atc Gy Ala Thr Val Gu Ile 135 gaa gct acc gca acc ggc G u Ala Thr Ala Thr G y 150 155 gta gcg aag gtc ttc ggc Val Ala Lys Val Phe G y 170 gaa gtc cca gtt act ttc G u Val Pro Val Thr Phe 185 t ca <210> 538 <211> 190 <212> PRT <213> Corynebacter i um gl <400> 538 IVet Ser I e Ser Arg Thr 1 5 Ser Ala Ala Leu Val Ala Val G n Arg Thr Asn G u Ala Ser Ser Thr Ser Thr Val u u Asp Val u 70 Sy u n Val Thr Phe Tyr Tyr Ala Ala lle Cys 100 Pro Ser Cys Thr y G u 115 Ser Asn Ser G n Pro G y 130 gca Al a gat Asp cct Pr o t cc Ser gca Al a 140 act Thr gat Asp gca Al a seql i st cor r ect ed. t xt gcg ttg gtg gac ggt gag cag gtt Ala Leu Val Asp y u n Val cca gag ggc ggc tac tac gca gcg Pro G u y y Tyr Tyr Ala Ala 95 100 ggt aac cca gtt cct tct tgc acc Sy Asn Pro Val Pro Ser Cys Thr 110 115 cag gca tgg ttg agc aac tcc cag G n Ala Trp Leu Ser Asn Ser G n 125 130 gaa gac ggc acc gca act gtg gag G u Asp G y Thr Ala Thr Val G u 145 ggc ttg gac tgc acc act cag gct G y Leu Asp Cys Thr Thr G n Ala 160 cat acc gaa ggt ttc cgc gat gtt His Thr G u G y Phe Arg Asp Val 175 180 gcc gct taagttttct taaaacgcac Al a Al a 190 ut ari cum Val Phe G y Cys Ser Pro 25 II e Leu Thr 40 Ser Ser Al a 55 Ile Val Val G u i e Ser Asp Ser Val 105 IVet Al a Asp 120 Al a Thr Val 135 I e Al a Al a 10 Pro His G n Thr Ser G n Thr Thr Thr Ser Pro Al a G y Leu Asp 90 Al a Asn Pr o Phe Thr Ser G u I I e Al a 140 Page 676 Thr G n Asn Ser Al a Pr o Sy G n 125 G u Al a Asp Pr o Ser Leu G u Asn 110 Al a Asp Al a Ser Thr Al a Val Sy Pr o Tr p Gy seql i st cor r ect ed. t xt Ala Thr Val G u Leu G u Ala Thr Ala Thr y Thr y Leu Asp Cys 145 150 155 160 Thr Thr G n Ala Cys Val Ala Lys Val Phe G y Asp His Thr G u G y 165 170 175 Phe Arg Asp Val Ala G u Val Pro Val Thr Phe Ala Ala Ala 180 185 190 <210> 539 <211> 1528 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1528) <223> RXN01209 <400> 539 cagattgcag cacagaaggc atcggcgcca ggcagctttg ctttatgacg tggatgccca ggctgtggcc tcgttggttg cggtggcgtt tattgatgcg tgc gag agg Cys Gu Arg gaa G u ttg Leu t cc Ser gaa G u cgc Ar g ct g Leu gca Al a aac Asn gt g Val t ac Tyr ggc Gy gga Gy gtt Val gt g Val ctt Leu gag G u gat Asp 120 ttg Leu act Thr cca Pr o tct Ser gcg Al a ctt Leu ct g Leu ctt Leu ttg Leu gat Asp gat Asp aaa Lys gt g Val gca Al a gtt Val 75 cac Hi s cca Pr o cat Hi s gt g Val ttt Phe aaa Lys gt g Val gca Al a gt c Val at t SIle gct Al a gct Al a at t SIle 140 tct Ser gt a Val cag G n 45 aag Lys aac Asn ggt Gy cat Hi s gt g Val 125 ggc Gy ttg Leu gct Al a ct g Leu gag G u gat Asp caa G n ctt Leu 110 at c SIle at t SIle t at Tyr 15 gga Gy cgc Ar g ct g Leu t ac Tyr ggc Gy 95 gaa G u gcg Al a ggt gty ggt Gy ct g Leu at t SIle gat Asp aaa Lys tta Leu 80 gat Asp ttg Leu cag G n ccg Pr o gt g Val 160 acc Thr gac Asp aac Asn ct c Leu at c SIle cct Pr o ttg Leu gcc Al a 130 gcc Al a ggc G y gat Asp agc Ser t ca Ser tgc Cys gcc Al a t at Tyr agc Ser 115 gag G u tct Ser at c SIle ccc Pr o gca Al a ggc Sy gat Asp gtt Val acg Thr 100 at t SIle act Thr act Thr gct Al a cct Pr o gtt Val att SIe gtg Val gct Al a gag G u caa G n gaa G u ggt G y gcg Al a gag 165 acc aaa cca gat gcg gca ccc gca ttg Thr Lys Pro Asp Ala Ala Pro Ala Leu Page 677 atc gcc gct gta gct caa gac Ile Ala Ala Val Ala n Asp seql i st cor r ect ed. t xt cac ggc atc gca tca gta gct att gga His y IIe Ala Ser Val Ala IIe y ggc Gy ggt Sy gcg Al a act Thr 230 tcg Ser ggt Gy ggc Sy ggc Sy ct g Leu 310 at g Met t cc Ser agc Ser ct g Leu gt g Val 390 cag G n ggt Gy ggt Gy tgc Cys 200 act Thr act Thr tct Ser gca Al a ggc Gy 280 aac Asn gcg Al a ggc Gy gag G u gat Asp 360 gt g Val tgc Cys cag G n cat Hi s 170 cgc Ar g gt c Val ct g Leu ct c Leu cca Pr o 250 at t l Ie tgc Cys at c SIle ttt Phe gcc Al a 330 ggt Sy ct a Leu gt c Val agt Ser ttt Phe 410 act Thr aac Asn tct Ser cgg Ar g tct Ser 235 cgt Ar g cag G n gtt Val cac Hi s t cc Ser 315 gac Asp ccc Pr o ct g Leu gat Asp gct Al a 395 gcg Al a ggc Gy gcg Al a gaa G u act Thr 220 caa G n gt g Val gct Al a gt g Val acc Thr 300 gat Asp acc Thr gt g Val gat Asp gt g Val 380 cct Pr o cgg Ar g gcg Al a gcc Al a at c SIle 205 gct Al a aca Thr ttg Leu gat Asp acc Thr 285 cca Pr o gt c Val gt c Val gt g Val gcg Al a 365 gt c Val gcc Al a act Thr ctt Leu gaa G u 190 at g Met t ttt Phe gaa G u tct Ser ct g Leu 270 tcg Ser ccc Pr o acc Thr gat Asp ctt Leu 350 agc Ser acc Thr at c l Ie cat Hi s gct Al a 430 gct Al a gcc Al a cct Pr o caa G n 240 gca Al a t cc Ser gt c Val acc Thr gat Asp 320 gt g Val ccc Pr o gaa G u aat Asn at g Met 400 acc Thr aac Asn gct Al a gcc Al a act Thr 225 gga Gy ggc Gy at t SIle gcg Al a ttt Phe 305 gcc Al a gct Al a gt c Val gaa G u at c SIle 385 gat Asp at c SIle gct Al a acg Thr aat Asn 210 ttc Phe gcc Al a act Thr gca Al a caa G n 290 ttg Leu at c l Ie t ca Ser at g Met tcg Ser 370 ccc Pr o gag G u gt c Val gt c Val ccc Pr o 195 cca Pr o tcg Ser ttc Phe gat Asp gca Al a 275 aac Asn gaa G u aag Lys tgg Tr p at c SI e 355 ct g Leu gaa G u gcc Al a at t SIle gt g Val 435 180 at c SIle gca Al a cct Pr o gt g Val ccc Pr o 260 ggt Gy acc Thr gaa G u ct c Leu ctt Leu 340 gcc Al a cgc Ar g ctt Leu at t l Ie gt c Val 420 cgc Ar g gac Asp gct Al a gaa G u aat Asn 245 aca Thr ggc Gy cac Hi s cag G n ggc Sy 325 ggt Sy acc Thr cgc Ar g gcc Al a gct Al a 405 aag Lys ccc Pr o 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 Page 678 gac ggc tcg gtg ttc cag gtg gaa a Asp y Ser Val Phe G n Val G u A 440 445 tcc cat ggc aca ggc tgt tcg ctc t Ser His G y Thr G y Cys Ser Leu S 455 460 gcc gcc ggc gaa agc gtg gaa Ala Ala y u Ser Val u 470 475 <210> 540 <211> 476 <212> PRT <213> Cor ynebact eri um gl ut ari cum 1 i st cor r ect ed. t xt iac ctg cgt gtc aac acc acc aac sn Leu Arg Val Asn Thr Thr Asn 450 ct gcg tca ctt gcc acc aag atc ier Ala Ser Leu Ala Thr Lys Ile 465 1459 1507 1528 <400> 540 Met 1 Val Val Lys G u Asp Thr Gy Cys Val 145 G u Ser Al a Al a Thr 225 Gy Cys u Arg Thr Asp Pro Asp Ser Al a Asn Ser G y Leu Cys Asp I e Al a Val Pro Tyr Thr 100 Leu Ser Ile 115 Al a G u Thr 130 Al a Ser Thr G y Ile Al a Val Ala lle 180 Thr Pro Ile 195 Asn Pro Al a 210 Phe Ser Pro Al a Phe Val Pr o 5 Val l Ie Val Al a G u G n G u Gy Al a G u 165 Gy Asp Al a G u Asn 245 G u Lys Tyr Leu Ser G u Ar g 70 Leu Al a Asn Val Thr 150 I le Gy Gy Al a Thr 230 Ser Gy Gy Asp 55 Gy Gy Ar g Leu Al a 135 Lys Al a Val Leu Al a 215 G n Pr o Val Thr 10 G y Pro 25 Val Ser Arg Ala Al a Leu Hi s Leu 90 Leu Leu 105 G n Leu Pro Asp Asp Al a Val Ala 170 Leu Arg 185 Val Val Arg Leu G u Leu Ala Pr o 250 Asp Lys Val Al a Val 75 Hi s Pr o Hi s Val Al a 155 G n Asn Ser Ar g Ser 235 Ar g Phe Lys Val Al a Val SIle Al a Al a SIle 140 Pr o Asp Al a G u Thr 220 G n Val Ser Val G n Lys Asn Gy Hi s Val 125 Gy Al a Hi s Al a SIle 205 Al a Thr Leu Leu Al a Leu G u Asp G n Leu 110 SIle SIle Leu Gy G u 190 bet Phe G u Ser Tyr Gy Ar g Leu Tyr Gy G u Al a Gy Gy I e 175 Leu Thr G n Leu SIle 255 Leu SIe Asp Lys Leu Asp Leu G n Pr o Val 160 Al a Al a Al a Pr o G n 240 Al a Sy Thr Asp Pro Thr Oy Oy Ala Gy lie On Ala Asp Leu Lys Ser Page 679 seqI i st car r ect ed. t xt 265 I Ie A a Ph e 305 A a A a ValI O31u I Ie 385 As p I Ie A a ValI Le u 465 A a 275 As n O31u Ly s Tr p I Ie 355 Le u O31u A a I Ie ValI 435 Thr Thr G31y Hi s O31n G31y 325 G31y Thr Ar g A a A a 405 Ly s Pr o As n Tyr ValI 295 O31u Le u Hi s G31y A a 375 Le u A a G31y G31y Hi s 455 G31y 280 As n A a G31y O31u As p 360 ValI cys O31n Hi s Ser 440 G31y [Vb t Cy s ValI Val Thr Ser Leu Val Thr ValI Ser Hi s 345 Ar g Hi s As p G31y Le u 425 ValI Thr Hi s Ser 315 As p Pr o Le u As p A a 395 A a G31y O31n Cys Thr 300 As p Thr ValI As p ValI 380 Pr o Ar g A a ValI Ser 460 285 Pr o ValI ValI ValI A a 365 ValI A a Thr Le u (31u 445 Le u Pr o Thr As p Le u 350 Ser Thr I Ie Hi s A a 430 As n Ser Le u ValI Le u 335 As p A a Pr o Thr As p 415 As p Le u A a Lys IlieAla Ala Gy Ou Ser Val Ou <210O> 541 <21 1> 1528 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1528) <223> FRXA01209 <400> 541 cagattgcag cacagaaggc atcggcgcca ct t tatgacg t ggat gccca ggctgtggcc gaa aag tac gt g act gat ttt t ct t O3 u Lys Tyr Val Thr Asp Phe Ser L ttg ggt ggc ggg cca aaa aaa gta g Leu G31y G31y G31y Pr o Lys Lys Val P t cc ggc gga gt t t ct gt g gt g cag c ggcagct t t g t cgt t ggt t g t g t at ct g .eu Tyr Leu 15 ict gga at t Il a 03 y IlIe 30 t g cgc gat Page 68C cggt ggcgt t t at tgat gcg at g t gc gag agg cct I'b t Cys O31u Ar g Pr o 1 gt c acc gat ccc gt t Val Thr As p Pr o Val gt t gac agc gca at t Val As p Ser Ala I Ie aag aac t ca ggc gt g Ser G y G y Val Ser Val Val G 4 seql i st cor r ect ed. t xt n Leu Arg Asp Lys Asn Ser G y Val 5 gaa G u cgc Ar g ct g Leu gca Al a aac Asn gt g Val acc Thr 150 at c SIle ggc G y ggt Sy gcg Al a act Thr 230 tcg Ser ggt G y ggc gcy ggc Gy gtt Val gt g Val ctt Leu gag G u gat Asp 120 ttg Leu cca Pr o gct Al a ggt Sy tgc Cys 200 act Thr act Thr tct Ser gca Al a ggc G y 280 aac Asn gcg Al a ctt Leu ct g Leu ctt Leu ttg Leu gat Asp gcg Al a gct Al a 170 cgc Ar g gt c Val ct g Leu ct c Leu cca Pr o 250 at t l Ie tgc Cys at c SIle gca Al a gtt Val 75 cac Hi s cca Pr o cat Hi s gt g Val gca Al a 155 caa G n aac Asn tct Ser cgg Ar g tct Ser 235 cgt Ar g cag G n gtt Val cac Hi s gca Al a gt c Val at t SIle gct Al a gct Al a at t SIle 140 ccc Pr o gac Asp gcg Al a gaa act Thr 220 caa G n gt g Val gct Al a gt g Val acc Thr 300 aag Lys aac Asn ggt G y cat Hi s gt g Val 125 ggc G y gca Al a cac Hi s gcc Al a at c SIle 205 gct Al a aca Thr ttg Leu gat Asp acc Thr 285 cca Pr o gag G u gat Asp caa G n ctt Leu 110 at c SIle at t SIle ttg Leu ggc G y gaa G u 190 at g Met t ttt Phe gaa G u tct Ser ct g Leu 270 tcg Ser ccc Pr o ct g Leu t ac Tyr ggc G y 95 gaa G u gcg Al a ggt G y ggt G y at c SIle 175 ct c Leu acc Thr caa G n ct c Leu at t SIle 255 aag Lys ct g Leu ttg Leu aaa Lys tta Leu 80 gat Asp ttg Leu cag G n ccg Pr o gt g Val 160 gca Al a gct Al a gcc Al a cct Pr o caa G n 240 gca Al a t cc Ser gt c Val acc Thr gaa G u gat Asp aca Thr ggt G y tgc Cys gt g Val 145 gag G u t ca Ser gct Al a gcc Al a act Thr 225 gga G y ggc G y at t SIle gcg Al a ttt Phe 305 ct c Leu at c SIle cct Pr o ttg Leu gcc Al a 130 gcc Al a ggc G y gt a Val acg Thr aat Asn 210 ttc Phe gcc Al a act Thr gca Al a caa G n 290 ttg Leu tgc Cys gcc Al a t at Tyr agc Ser 115 gag G u tct Ser at c SIle gct Al a ccc Pr o 195 cca Pr o tcg Ser ttc Phe gat Asp gca Al a 275 aac Asn gaa G u gat Asp gtt Val acg Thr 100 at t SIle act Thr act Thr gct Al a at t l Ie 180 at c SIle gca Al a cct Pr o gt g Val ccc Pr o 260 ggt G y acc Thr gaa G u gct Al a gag G u caa G n gaa ggt G y gcg Al a gag G u 165 gga G y gac Asp gct Al a gaa G u aat Asn 245 aca Thr ggc G y cac Hi s cag G n 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 ctg gaa gcg gtc ttt tcc gat gtc acc gtc gat gcc atc aag ctc ggc Page 681 Leu G u Ala Val Phe Ser Asp 310 315 seql i st cor r ect ed. t xt Val Thr Val Asp Ala II 320 e Lys Leu G y 325 atg ttg ggc tct gcc gac acc gtc gat IVet Leu y Ser Ala Asp Thr Val Asp 330 tcc cac gag cac ggt ccc gtg gtg ctt Ser His G u His G y Pro Val Val Leu 345 350 agc ggt gat cgc cta ctg gat gcg agc Ser y Asp Arg Leu Leu Asp Ala Ser 360 365 ctg gcc gtg cac gtc gat gtg gtc acc Leu Al a Val His Val Asp Val Val Thr 375 380 gtg ttg tgc gac agt gct cct gcc atc Val Leu Cys Asp Ser Ala Pro Ala lle 390 395 cag gct cag gga ttt gcg cgg act cat G n Ala G n y Phe Al a Arg Thr Hi s 410 ggt gga cat ctg act ggc gcg ctt gct G y G y Hi s Leu Thr G y Ala Leu Al a 425 430 gac ggc tcg gtg ttc cag gtg gaa aac Asp y Ser Val Phe G n Val G u Asn 440 445 tcc cat ggc aca ggc tgt tcg ctc tct Ser His G y Thr G y Cys Ser Leu Ser 455 460 gcc gcc ggc gaa agc gtg gaa Ala Ala y u Ser Val u 470 475 <210> 542 <211> 476 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 542 IVet Cys G u Arg Pro G u Lys Tyr Val 1 5 Val Thr Asp Pro Val Leu y y y 25 Val Asp Ser Ala Ile Ser y y Val 40 Lys Asn Ser y Val G u Asp Val Arg 55 Su Leu Cys Asp Ala Arg y Val Ala 70 Asp IIe Ala Val G u Leu G y Leu Hi s gt g Val ccc Pr o gaa G u aat Asn at g Met 400 acc Thr aac Asn cgt Ar g r r r gct Al a gt c Val gaa G u at c SIle 385 gat Asp at c SIle gct Al a gt c Val ctt Leu 465 Phe Lys Val A1 a Val I le t ca Ser at g Met tcg Ser 370 ccc Pr o gag G u gt c Val gt c Val aac Asn 450 tgg Tr p at c SI e 355 ct g Leu gaa G u gcc Al a at t SIle gt g Val 435 acc Thr ctt Leu 340 gcc Al a cgc Ar g ctt Leu at t SIe gt c Val 420 cgc Ar g acc Thr 1123 1171 1219 1267 1315 1363 1411 1459 1507 1528 gcg tca Al a Ser Thr Asp 10 Pro Lys Ser Val Al a Al a Leu Val Leu Hi s 90 Page 682 gcc acc aag atc Ala Thr Lys Ile Ser Val G n Lys Asn Sy Leu Al a Leu a u Asp G n Tyr Sy Ar g Leu Tyr Sy seqi i st car r ect ed. t xt Th r Pr o Ty r Th r O31n Ala Ar g O31u L eu L eu Pr o Ala Hi 100 105 s Leu 03 u Leu 110 O31y CYS ValI 145 O31u Ser A a A a Thr 225 O31y Oy le A a Ph e 305 A a A a ValI O31u I Ie 385 As p I Ie A a ValI Ser 115 O31u Ser I Ie A a Pr o 1 95 Pr o Ser Ph e As p A a 275 As n O31u Ly s Tr p I Ie 355 Le u O31u A a I Ie ValI 435 Thr O31u O31y A a O31u 1 65 O31y As p A a O31u As n 245 Thr O31y Hi s O31n O31y 325 O31y Thr Ar g A a A a 405 Ly s Pr o As n Le u A a 135 Ly s A a ValI Le u A a 215 O31n Pr o O31y Tyr ValI 295 (31u Le u Hi s O31y A a 375 Le u A a O31y O31y Hi s 455 As p 120 Le u Pr a A a O31y cys 200 Thr Thr Ser A a O31y 280 As n A a O31y (31u As p 360 ValI cys O31n Hi s Ser 440 (31y O31n Pr a As p ValI Le u 1 85 ValI Ar g O31u A a O31y 265 vb t Thr ValI Ser Hi s 345 Ar g Hi s As p O31y Le u 425 ValI Thr Le u As p A a A a 1 70 Ar g ValI Le u Le u Pr a 250 li1e cys I Ie Ph e A a 330 O31y Le u ValI Ser Ph e 410 Thr Ph e Hi s ValI A a 1 O31n As n Ser Ar g Ser 235 Ar g O31n ValI Hi s Ser 315 As p Pr a Le u As p A a 395 A a O31y O31n A a I Ie 140 Pr a As p A a O31u Thr 220 O31n ValI A a ValI Thr 300 As p Thr ValI As p ValI 380 Pr a Ar g A a ValI ValI 125 O31y A a Hi s A a I Ie 205 A a Thr Le u As p Thr 285 Pr a ValI ValI ValI A a 365 ValI A a Thr Le u O31u 445 le le Le u O31y O31u 1 vb t Ph e O31u Ser Le u 270 Ser Pr a Thr As p Le u 350 Ser Thr I Ie Hi s A a 430 As n A a O31y Oy le 1 Le u Thr O31n Le u I Ie 255 Ly s Le u Le u ValI Le u 335 As p A a Pr a Thr As p 415 As p Le u (3y Cys Ser Leu Ser Ala Ser 460 Page 683 seql i st cor r ect ed. t xt Leu Ala Thr Lys Ile Ala Ala Gy Ou Ser Val u 465 470 475 <210> 543 <211> 723 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(700) <223> RXN01413 <400> 543 tttgccctat tggaagaagt aaattccaca ccttcacttt ccaatacttc ttttggtgag cgggtttcct cagcgtttaa ccatctgaaa ccatctgaga ttg tta Leu caa G n tcg Ser t cc Ser gt c Val aag Lys gag G u ggc Gy ttc Phe acc Thr 150 gt a Val gat Asp ct c Leu act Thr aat Asn acg Thr acc Thr gag G u ggc Gy aac Asn 120 gag G u gca Al a cca Pr o gtt Val gag G u cat Hi s ct g Leu aaa Lys aat Asn gt c Val cag G n gt g Val aag Lys gtt Val gca Al a 170 t cc Ser tta Leu ttc Phe gga Gy caa G n ttc Phe gac Asp gt a Val gt g Val ggt Gy 140 acc Thr gca Al a gca Al a gt a Val cgg Ar g ttg Leu 45 ggg Gy gag G u ct c Leu gt c Val ctt Leu 125 gaa G u gca Al a aag Lys cct ct g Leu ct g Leu aag Lys gt a Val gct Al a aag Lys gt c Val 110 ttc Phe gag G u gag G u cgt Ar g ttt ggt Gy 15 cca Pr o at a SIle gaa G u t cc Ser gag G u 95 aag Lys gac Asp cgc Ar g ct a Leu gt g Val 175 gtt Val ct g Leu cga Ar g ctt Leu acc Thr 80 gct Al a ggt Gy gga Gy gtt Val gca Al a 160 gt c Val acc cat ctg ttc Thr Hi s Leu Phe caa gat ggt tac G n Asp G y Tyr cat ctc cgt aac His Leu Arg Asn tgt gaa ctt tat Cys G u Leu Tyr cct caa gca acc Pro G n Ala Thr tcc ggc ctt gag Ser G y Leu G u cgc ctc att tat Arg Leu lie Tyr 100 atg gac ttc ccc IVet Asp Phe Pro 115 t cc tac cac gtc Ser Tyr His Val 130 ggc gca gtc tgc Gy Ala Val Cys ggt gct gag gtt G y Al a u Val 165 cgt gcg gtt caa Arg Ala Val G n 180 Pro Phe acc tcc gta tgg ctt gct gag Thr Ser Val Trp Leu Ala G u Page 684 seql i st cor r ect ed. t xt 185 190 gac aac aag tagagtttta aaataccgat caa Asp Asn Lys 200 <210> 544 <211> 200 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 544 Leu Thr H 1 Gn n A G n Hi s L Trp Cys G Leu Pro G Leu Ser G Al a Ar g L G y lMt A 1 Ser Ser T 130 Ser G y A 145 Lys G y A Thr Arg A Val Trp L is Leu Phe 5 sp G y Tyr eu Arg Asn Su Leu Tyr In Al a Thr ly Leu G u eu IIe Tyr 100 sp Phe Pro 15 yr His Val Ia Val Cys I a G u Val 165 la Val n 180 eu Al a G u Leu G u Leu Asp Tr p Pr o Thr 55 Val Leu G n G u Ser 135 Phe Asp Al a Asn G u 25 Asp Sy Pr o Thr Pr o 105 Al a Pr o Al a Val Al a 185 G u 10 Hi s Leu Lys Asn Val 90 G n Val Lys Val Al a 170 Ser Ar g Leu G n Asp Asn G u Tyr Asp SIle SIle 155 Thr Asn Leu Phe Sy G n Phe Asp Val Val Sy 140 Thr Al a Al a Val Ar g Leu Sy G u Leu Val Leu 125 G u Al a Lys Pr o Leu Leu Lys Val Al a Lys Val 110 Phe G u G u Ar g Phe 190 Sy Pr o SIe G u Ser G u Lys Asp Ar g Leu Val 175 Thr <210> 545 <211> 795 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(772) <223> RXN01617 <400> 545 tcagaagcta ccggcggcgc cggcatccag gttgatttga agaccttcca gcatttaaga tgtttatggc attgggtcca tcacatgctt gggtggcctt ttg atc cta aag aca 115 Leu Ile Leu Lys Thr Page 685 seql i st cor r ect ed. t xt act Thr cag G n ggt Gy gag G u aag Lys gcg Al a gcc Al a aag Lys gtt Val ttc Phe 150 gag G u gag G u cgc Ar g at c SIle gag G u ttg Leu aac Asn cag G n gt g Val acc Thr gca Al a 120 ggt Gy ggc Gy gt c Val gcc Al a gt c Val 200 gtt Val gcc Al a act Thr ttc Phe ccc Pr o cca Pr o tct Ser cgc Ar g at c SIle gac Asp ggc Gy 170 ggc Gy cgc Ar g tt g Leu acc Thr cct Pr o aag Lys ggc Gy 75 cag G n ggc Gy ct c Leu gac Asp tac Tyr 155 gct Al a aac Asn gca Al a t cc Ser gca Al a gca Al a cac Hi s gcg Al a gca Al a ct a Leu at t SIle ttc Phe 140 cac Hi s ggc Gy tct Ser gt g Val cgg Ar g gcg Al a acg Thr 45 gtt Val gca Al a acc Thr gac Asp cat Hi s 125 cca Pr o gt g Val tgt Cys gcc Al a aag Lys 205 ttt Phe cac Hi s at c SIle gt c Val ctt Leu gt g Val aag Lys 110 gag G u ggc Gy ttc Phe acc Thr gtt Val 190 gac Asp gat Asp 15 gat Asp gat Asp ct a Leu gat Asp gtt Val 95 ct g Leu caa G n gac Asp tct Ser ttc Phe 175 gat Asp gct Al a gcg cag gtt Al a G n Val ctt gat gtg Leu Asp Val act gtg gca Thr Val Al a gac ccg gta Asp Pro Val act gac act Thr Asp Thr act cca aac Thr Pro Asn gag acc at c Su Thr Ile gga cct cag G y Pro G n 130 aac gct gtg Asn Al a Val 145 gaa cca aag u Pro Lys 160 gca gct gt c Al a Al a Val gca gtg acc Al a Val Thr gt c gca t cc Val Al a Ser 210 at c gct I e Al a gtg aag Val Lys acc gct Thr Al a ct g at c Leu II e gcc ctt Al a Leu aac ttc Asn Phe 100 gac gac Asp Asp 115 tac gtc Tyr Val gac gta Asp Val atc ggc I e y atc acc Ile Thr 180 acc gct Thr Al a 195 aac gca Asn Ala ttt acc tct gtg tgg ttg gcg gaa gac aac aag tagaaatctt aaacaagctc 792 Phe Thr Ser Val Trp Leu Ala G u Asp Asn Lys 215 220 cct 795 <210> 546 <211> 224 <212> PRT <213> Corynebacter i um gl utam cum <400> 546 Leu Ile Leu Lys Thr Thr y Ile Thr Val Leu Ser Arg Phe Asp Ala Page 686 seql i st cor r ect ed. t xt Asn n IIle G u G n Asp Val Pr o Asp Pr o Thr Pr o Al a 145 Pr o Al a Val Al a I e Al a Val Lys Thr Al a Leu II e Al a Leu Asn Phe 100 Asp Asp 115 Tyr Val Asp Val I e G y lIe Thr 180 Thr Al a 195 Asn Al a SIle Leu Cys Ar g G u Leu Val Leu Asp 165 Al a Lys Pr o Gy G u Lys 70 Al a Al a Lys Val Phe 150 G u G u Ar g Phe Mbt G u Gy Lys Thr G u Lys 135 Asp Ar g Leu Val Thr 215 Leu 40 Asn G n Val Thr Al a 120 Gy Gy Val Al a Val 200 Ser Al a Al a Thr 25 Sy Thr Pro Ser Phe Lys G u Pro G y Leu Pro G n 90 Leu Ser G y 105 Al a Ar g Leu G y I e Asp Thr Asp Tyr 155 Ser G y Al a 170 Lys G y Asn 185 Thr Arg Ala Val Trp Leu Al a Al a Hi s Ala Thr Ile Hi s Val Val Al a Al a Leu Al a Thr Val Leu Asp Lys 110 Ile His G u 125 Phe Pro G y 140 Hi s Val Phe G y Cys Thr Ser Al a Val 190 Val Lys Asp 205 Al a u Asp 220 Asp Asp Leu Asp Val Leu G n Asp Ser Phe 175 Asp Al a Asn <210> 547 <211> 638 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA01617 <400> 547 gct aat cag att gag gcc gcc acc gca gcg cac gat ctt gat gtg gtg Ala Asnn G lle Gu Ala Ala Thr Ala Ala His Asp Leu Asp Val Val 1 5 10 aag atc ggt atg ttg ggt act cct gca acg atc gat act gtg gca acc Lys Ile y IVet Leu y Thr Pro Ala Thr Ile Asp Thr Val Ala Thr 25 gct ttg gag gaa aac agc ttc aag cac gtt gtc cta gac ccg gta ctg Ala Leu G u G u Asn Ser Phe Lys His Val Val Leu Asp Pro Val Leu 40 Page 687 at c t gc I I e Cys ct t cgc Leu Arg ttc gag Phe G3 u gac ct g Asp Leu gt c gt t Val Val gt a ct t Val Leu 130 ggc gac G yAs p 145 acc gca Thr Al a gct aag Al a Lys gca ccg Al a Pr o aag ggc cag gag ccc gg Lys Oy On Ou Pro G1 55 seql i st car r ect c gcg gca ctt y Al a Al a Leu ed. t xt gcg Al a gcc Al a aag Ly s gt t ValI 115 tt c Ph e gag G u gag 0Ou cgc Ar g tt t Ph e 1 95 gt g ValI ac c Thr gca Al a ggt G31y ggc G31y gt c ValI gcc Al a 1 65 gt c ValI ct g Le u 70 ct g Le u gcc Al a ggc G31y acc Thr t cc Ser 150 aag Ly s acc Thr cca Pr o t ct Ser cgc Ar g at c I Ie g ac As p 135 ggc G y ggc 0Gy cgc Ar g c ag (31n G31y ct c Le u g ac As p 120 t ac Tyr gct Al a aac As n gca Al a tt g Le u 200 gca Al a ct a Le u at t I Ie 1 05 tt c Ph e c ac Hi s ggc G y t ct Ser gt g ValI 1 85 ac c Thr g ac As p 90 cat Hi s cca Pr o gt g ValI t gt Cys gcc Al a 1 70 aag Ly s gt g ValI aag Ly s gag O31u G31y tt c Ph e ac c Thr 1 gt t ValI g ac As p g at As p gt t ValI ct g Le u c aa O31n g ac As p t ct Ser 140 tt c Ph e g at As p gct Al a act Thr act Thr gag O31u gga G31y aac As n 125 g aa O31u gca Al a gca Al a gt c ValI aag Ly s 205 cca Pr o ac c Thr cct Pr o 110 gct Al a cca Pr o gct Al a gt g ValI gca Al a 1 gac act gcc As p Th r Al a aac As n at c lIe c ag O31n gt g ValI aag Ly s gt c ValI ac c Thr 1 t cc Ser t ct gt g t gg Ser Val Tr p gcg gaa gac aac Al a 03 u Asp As n t agaaat ct t aaacaagct c cct <210> 548 <211> 205 <21 2> PRTF <21 3> Cor ynebact er i urn gI ut ani cumn <400> 548 Al aAsn O n Il e 0 u Al aAl aThr Al a 1 5 Lys I Ie G31y I'bt Leu G31y Thr Pro Al a Al a Leu O31u O31u Asn Ser Phe Lys Hi s 40 Ilie Cys Lys G31y O31n 31u Pro G31y Al a Leu Ar g Al a Lys Val Leu Pr o O31n Al a 70 Phe O31u Al a Thr Thr Leu Ser G31y Leu Asp Leu Lys O31u Ala Al a Arg Leu Ilie Al a Hi s 10 Thr I Ie Val Val I Al a Leu Thr Val 75 As p Lys 1 90 Hi s 03 u Page 688 Le u Thr As p Thr Thr O31u G31y ValI Al a ValI Thr As n I Ie O31n ValI Th r Le u Al a As n As p Ty r Val Val Gy 145 Thr Al a Al a Val 115 Phe G u G u Ar g Phe 195 Gy Gy Val Al a 165 Val Ser SIle Asp 135 Gy Gy Ar g Tr p seql i st cor r ect ed. t xt 105 Asp Phe Pro y Asp Asn 120 125 Tyr His Val Phe Ser G u 140 Ala y Cys Thr Phe Ala 155 Asn Ser Ala Val Asp Ala 170 Ala Val Lys Asp Ala Val 185 Leu Ala G u Asp Asn Lys 200 205 110 Al a Pr o Al a Val Al a 190 Val Asp Lys I e Val lie 160 Thr Thr 175 Ser Asn <210> 549 <211> 915 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(892) <223> RXS01807 <400> 549 gctcaccgag ctggacacca agctccgcgc agtgcaggaa gcagtggctg gaactcggcg aggaaatcga gggctagttc gaacacggcg agctggaaat gag G u gcc Al a ct c Leu gct Al a cac Hi s ct g Leu t at Tyr aaa Lys at t SIle gt g Val gct Al a ccc Pr o cag G n gat Asp acc Thr cgc Ar g gag G u at g Met at t l Ie gt g Val at g Met ggg Gy gct Al a acc Thr cgc Ar g ggc Gy gt g Val tta Leu gac Asp at c SIle cag G n ccg Pr o gca Al a agc Ser at g Met 45 agt Ser caa G n gat Asp gt g Val cat Hi s cag ctg G n Leu 15 cag gtg G n Val cac aac His Asn t cc at g Ser 'bet tgg ctc Trp Leu gag gtt G u Val 95 cgt gtg Arg Val 110 gtg cgc Val Arg gag G u gtt Val gaa G u ccg Pr o gcc Al a 80 t cc Ser gt c Val at g Met 1 ttt Phe t at Tyr ggc Gy cgt Ar g gac Asp acc Thr gct Al a ccg tcg gca ggc Pro Ser Ala Gy gat cag cgc cgc Asp G n Arg Arg ggt tcc gtg ggg Sy Ser Val y ctc cgc gtg gtc Leu Arg Val Val tat gca agt tct Tyr Ala Ser Ser gcg ctg caa gac Ala Leu G n Asp att tcc acc ggc ile Ser Thr G y 100 gcg tgg ctg cag Ala Trp Leu G n 115 atc gtg gtg gat ccc atc lie Val Val Asp Pro Ile Page 689 seql i st cor r ect ed. t xt 120 125 130 atg ggg gac agt gac gtg gga att tat gtc gcc gac gag atc gca acc 547 IVet y Asp Ser Asp Val Gy I e Tyr Val Ala Asp u I e Ala Thr 135 140 145 gcc atc tgc cag gac tta tgc cct ctg gct acc gga atc att ccc aat 595 Al a lie Cys n Asp Leu Cys Pro Leu Al a Thr G y lie lie Pro Asn 150 155 160 165 gct ttc gag ctc tcc cac atg gtt ggc tcc ggc gat ccg cgc tcg ctg 643 Ala Phe G u Leu Ser His Met Val G y Ser G y Asp Pro Arg Ser Leu 170 175 180 ctc ggc ccg ttt ggc gag tgg atc atc atc acc agc gcc act gaa act 691 Leu y Pro Phe y u Trp Ile Ile Ile Thr Ser Ala Thr u Thr 185 190 195 gtg ggc acc acc gtc acc cgc atc gtc acc cgt gac agc gtc cag gaa 739 Val y Thr Thr Val Thr Arg lie Val Thr Arg Asp Ser Val n G u 200 205 210 atc gcc tcc gcc acc gtc gat acc acg gcc aaa ggg gca ggc gac gtc 787 Ile Ala Ser Ala Thr Val Asp Thr Thr Ala Lys y Ala y Asp Val 215 220 225 tac gcc gca gca tta atc gcc gcc ctg cat aaa gat ttt tcg ctt atc 835 Tyr Ala Ala Ala Leu Ile Ala Ala Leu His Lys Asp Phe Ser Leu Ile 230 235 240 245 gac gcc gcc agc cac gca tcc aac acc gtc tgc gcc ggc ctg cag acc 883 Asp Ala Ala Ser His Al a Ser Asn Thr Val Cys Ala G y Leu G n Thr 250 255 260 aaa gcg ctt taggtttcgt ccgtctctga cag 915 Lys Ala Leu <210> 550 <211> 264 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 550 IVet Pro Ser Ala Gy Ou Ou Ile Leu Ou n Arg Ala n Leu u 1 5 10 Phe Asp n Arg Arg Al a Asp Val Val Iebt I e y Ser G n Val Val 25 Tyr G y Ser Val G y Leu Ser Ala Ala lie Pro Val IVet His Asn G u 40 Sy Leu Arg Val Val Ala Val Pro Thr Val Val Leu Ser Ser Met Pro 55 Arg Tyr Ala Ser Ser His Arg G n Pro IVet Ser Asp G n Trp Leu Ala 70 75 Asp Ala Leu G n Asp Leu Val Asp Leu y lie lie Asp G u Val Ser 90 Thr lie Ser Thr y Tyr Phe Thr Ser Ala Ser G n Val Arg Val Val 100 105 110 Al a Ala Trp Leu G n Lys Ile Arg G u Thr His Pro His Val Arg Ile 115 120 125 Page 690 Val Asp 145 Gy Asp Ser Asp Gy 225 Asp Al a Asp I le I le Ar g Thr 195 Val Gy Ser Leu SIle Thr Asn 165 Leu Thr G u Val SIle 245 Thr Mbt Al a 150 Al a Leu Val SIle Tyr 230 Asp Lys Gy 135 l le Phe Gy Gy Al a 215 Al a Al a Al a seql i st cor r ect ed. t xt Asp Ser Asp Val Gy Ile 140 Cys G n Asp Leu Cys Pro 155 G u Leu Ser His bet Val 170 Pro Phe Gy u Trp lie 185 Thr Thr Val Thr Arg lie 200 205 Ser Ala Thr Val Asp Thr 220 Ala Ala Leu i e Ala Al a 235 Ala Ser His Ala Ser Asn 250 Leu Val Al a Ser 175 I e Thr Al a Hi s Val 255 <210> 551 <211> 622 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(622) <223> RXC01021 <400> 551 cgagaggctt ttttggctct aagcctttta gtcgtgcgaa gtgccaccga gatcgattgg tcgctgtaag gtatctgatt agc Ser cgc Ar g t cc Ser gt c Val ttt Phe aca Thr cgt Ar g gct Al a caa G n cag G n gtt Val gcg Al a gaa G u t ca Ser gac Asp tct Ser gat Asp gat Asp ggc Gy acg Thr gct Al a gaa G u gca Al a 75 gaa G u tcg Ser ggg Gy gcc Al a cgt Ar g gac Asp gcg Al a cag G n gct Al a gga Sy 45 cgt Ar g act Thr ct g Leu cca Pr o cct Pr o gtt Val gca Al a gga Sy aaa Lys gca ccg Ala Pro 15 gcg gca Al a Al a ctt gag Leu G u gag at c G u Ile tca aac Ser Asn 80 ctg ggg Leu G y Page 691 cgaaatct t a agcagcctcg atg tcc agt tcc gaa let Ser Ser Ser G u 1 tct gta cag cct gaa Ser Val G n Pro G u gct tcc aag gaa gct Ala Ser Lys G u Ala tgg gcc agg acc gct Trp Ala Arg Thr Ala aat gca ctg aat gtc Asn Ala Leu Asn Val atg acc tac acc atg let Thr Tyr Thr let gag ttg ggt gat gtc G u Leu G y Asp Val gca A a gga O31y gca A a t cc Ser 1 50 ggc O31y at t I Ie t ct Ser 120 gct A a gct A a gt t ValI gag O31u gt a ValI O31y t cc Ser act Thr 1 gct A a gt c ValI gt t ValI ct a Le u 155 gt g ValI tt g Le u ct t Le u at t I Ie 140 gt t ValI tt g Le u seqI i st car r ect ed. t xt 95 gct gt t ggt t ct gt g cgt Ala Val O31y Ser Val Ar g 110 agt cag gt c ct t cgc gct Ser O31n Val Leu Ar g Ala 125 130 gat ggc cac aca at c caa Asp O31y Hi s Thr Ilie O31n 145 gat cgc gca at t aca gat Asp Ar g Ala Ilie Thr Asp 1 60 cgt t ct Ar g Ser gga O31y 115 at t I Ie g aa O31u cct Pr o 1 00 gcc A a gct A a gcg A a gt g ValI cga Ar g c ag O31n ct a Le u gag O31u 1 <210O> 552 <211> 174 <21 2> PRT <213> Corynebacter iurn gi ut ani curn <400> 552 I'bt Ser Ser Ser 0 u Ser Ser Ser A a Tr p As n vb t (31u ValI Ar g I Ie 145 Thr O31n Lys5 Ar g Le u Tyr O31y O31y 115 li1e O31u Pr o O31u A a A a ValI vb t ValI Ar g O31n Le u O31u 1 Ar g Ser ValI Ph e 70 Thr A a O31y A a Ser 150 O31y Ar g O31n O31u Pr o A a Ar g As n A a 135 li1e Thr Ar g A a O31n 40 O31n ValI A a I Ie Ser 120 A a A a ValI Ser As p vb t Le u Pr o Le u Thr 1 O31y As p Ar g ValI O31y Thr A a O31u A a O31u A a ValI ValI Le u 1 ValI Ser O31y A a Ar g As p A a Le u Le u I Ie 140 ValI Le u O31n A a O31y Ar g Thr Le u A a Ser 125 As p As p Ar g A a A a Le u O31u Ser Le u O31y ValI Hi s A a <210O> 553 <211> 1107 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn Page 692 seqi i st car r ect ed. t xt <220> <221> CDS <222> (101)..(1084) <223> RXN02246 <400> 553 t gccgacgct ggcgt ggat g cct t cgt t gc aggt t ccgct gtgtacggcg ctgaggatcc caacaaggcg atccaggagt tgcgagcact cgcgcagt aa gcg Al a agc Ser gt c Val gt g Val gt g Val t cc Ser gcg Al a gcg Al a ccc Pr o ttt Phe 150 cag O31n agt Ser aat Asn ccc Pr o gat Asp aat Asn ggc G31y gcg Al a acc Thr gcg Al a ccc Pr o gt c Val 120 ct g Leu t cc Ser at t I Ie aga Ar g t cc Ser 200 cgc Ar g gcc Al a cca Pr o ggc G31y gca Al a gag (31u ct C Leu ccg Pr a acg Thr gcg Al a gt g Val ggg G31y 170 gcg Al a acg Thr gt t Val cac Hi s gt c Val gcc Al a gct Al a ccg Pr a 75 gac Asp t ca Ser cat Hi s acg Thr gac Asp 155 ccg Pr a at c SI e gcg Al a at t SI e cac Hi s ggc G31y acg Thr gcc Al a t gc Cys gcc Al a gcc Al a ttt Phe cgt Ar g 140 ggt G31y gat Asp at c l Ie cgg Ar g ggc 31y 220 gt g Val gct Al a gca Al a 45 gga G y aac Asn ggg G3y gct Al a tta Leu 125 ct g Leu ttt Phe gcg Al a gt g Val acc Thr 205 t cc Ser t ca Ser gt c Val cct Pr a gt g Val cat Hi s at c l Ie ggg G31y 110 gat Asp ggc G31y gct Al a cgg Ar g ggt G31y 190 gat Asp cgc Ar g caa O31n ttg Leu ggt G31y gcc Al a ggc G31y 80 cac Hi s ggt G31y cgg Ar g ccc Pr a gcc Al a 160 ttt Phe ggt G31y ggt G31y gt t Val at g lvb t 1 gt c Val gac Asp ggc G31y aac Asn cgc Ar g gt g Val gcc Al a at c l Ie cat Hi s 145 acc Thr gt g Val act Thr ct t Leu cca Pr a 225 gat gtt gcg Asp Val Ala cga Ar g gcc Al a ccg Pr a ggg G y acg Thr ttt Phe ttt Phe agg Ar g 130 gt c Val gat Asp cac Hi s gcg Al a t at Tyr 210 gca Al a ggc G31y gac Asp cac Hi s ggc G31y ggt G31y t ac Tyr ttg Leu 115 gca Al a acg Thr ggc G31y gaa O31u ttg Leu 195 gaa O31u gat Asp acc Thr ggc G31y gcc Al a acg Thr cca Pr a gcc Al a 100 gcg Al a ct g Leu ttg Leu acc Thr gat Asp 180 act Thr aat Asn t cc Ser cac Hi s acc Thr gag O31u gaa O31u gcg Al a t gt Cys aat Asn gag O31u gag O31u aag Lys agc Ser 165 cga Ar g gat Asp caa O31n aac Asn Page 693 ttg Leu 230 t ca Ser cca Pr o cag G n aac Asn acg Thr 310 aag Lys cgc Ar g ct g Leu tta Leu t at Tyr 280 cca Pr o gt g Val cac Hi s seql i st cor r ect ed. t xt ttg gga tat gag cag tac gcg gga ata cca Leu y Tyr u n Tyr Ala Gy lie Pro 235 240 tgg gat aaa ggg tgc cga gac att tta atc Trp Asp Lys y Cys Arg Asp lie Leu lie 250 255 gct ggg gca gcg ctg cgc tta ggc att gtt Ala Gy Ala Ala Leu Arg Leu Gy Ile Val 265 270 gtt gcc ccc gct ttg ttg ggc gct gga cga Val Ala Pro Ala Leu Leu Gy Ala Gy Arg 285 290 caa gaa acc acg atg gat cag att atg cgt Sn u Thr Thr IVet Asp n Ile IVet Arg 300 305 aga cag ttg ggt tca gat gta ttg ata gaa Arg G n Leu y Ser Asp Val Leu Ile G u 315 320 taaatgttca caggtattgt cga gag G u gaa G u gat Asp 275 t ca Ser ttt Phe at g Met gct Al a ggt Gy 260 cag G n gt g Val gac Asp at g Met 835 883 931 979 1027 1075 1107 <210> 554 <211> 328 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 554 IVet Asp Val Ala His Ala Leu Asp Leu 1 5 Val Arg y Thr Thr Ser Pro Asn Pro 25 Asp Ala Asp y G u Val Val y Val 40 G y Pro His Ala G u Val Val Ala Leu 55 Asn y y Thr Ala Val Val Thr Leu 70 Arg Thr y Pro Cys Ser Lys Ala Leu Val Phe Tyr Ala Asn Ala Asp Pro Phe 100 105 Ala Phe Leu Ala G u Al a y Val Asp 115 120 lie Arg Ala Leu G u Pro Trp Leu Val 130 135 His Val Thr Leu Lys Phe Ala Ser Thr 145 150 Thr Asp y Thr Ser G n Trp lie Thr 165 Al a 10 Pr o Sy Al a G u Leu 90 Pr o Thr Al a Val Sy 170 Hi s Val Al a Al a Pr o Asp Ser Hi s Thr Asp 155 Pr o Hi s Sy Thr Al a Cys Al a Al a Phe Ar g 140 Sy Val Al a Al a Sy Asn Sy Al a Leu 125 Leu Phe Ser Val Pr o Val Hi s SIle Sy 110 Asp G y Al a Asp I e Pr o Ar g Tyr Al a Sy G u Ar g Sy Al a 175 Asp Al a Ar g Page 694 ValI Thr Le u Pr o 225 li1e Le u I Ie G31y vb t 305 li1e O31u Le u 1 95 O31u As p O31u O31u As p 275 Ser Ph e vb t Ar g As p O31n As n Le u 245 O31y ValI I Ie Thr Ar g 325 Ly s Pr o Ar g 215 A a A a Thr A a Tr p 295 Ser (31u seqI i st car r ect ed. t xt Arg Asp Ala Ilie lieVal 1 Ser Leu Thr Ala Ar g Thr 200 205 Ar g Val Val I Ie G31y Ser 220 Ar g Leu G31y Ty r O31u O31n 235 Leu Tr p As p Lys (31y Cys 250 Leu A a 03 y A a A a Leu 265 Ty r Val Ala Pr o Ala Leu 280 285 Pro O31n O31u Thr Thr I'b t 300 Val Ar g O31n Leu G31y Ser 315 Hi s Thr Thr O31u A a As p 255 Le u O31y O31n ValI <210O> 555 <21 1> 1107 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1084) <223> FRXA02246 <400> 555 t gccgacgct ggcgt ggat g cct t cgt t gc aggt t ccgct caacaaggcg at ccaggagt t gcgagcact cgcgcagt aa gcg A a ag c Ser gt c ValI gt g ValI gt g ValI g at As p aat As n ggC O31y gcg A a ac c Thr gcc A a cca Pr o O31y gca A a gag O31u cac Hi s gt c ValI gcc A a gct A a ccg Pr o 75 c ac Hi s ggc O31y ac g Thr gcc A a t gc Cys gt g ValI gct A a gca A a 45 gga O31y aac As n gat caa As p O31n 15 at t tt g I I e Leu cct ggt Pro O31y cgt gcc Ar g Ala t ac ggc Ty r 03 y 80 Page 69E gt gt acggcg ct gaggat cc at g gat gt t gcg cac I'b t Asp Val Ala Hi s 1 gt c cga ggc acc acc Val Ar g O31y Thr Thr gac gcc gac ggc gag Asp A a Asp 03 y 03 u ggc ccg cac gcc gaa O31y Pr o Hi s Ala O31u aac ggg ggc acg gcg Asn 03 y 03 y Thr A a cgc acg ggt cca t gt Ar g Thr O31y Pr o Cys seqi i st car r ect ed. t xt tcc aag gcg ctt ctc gac gcc ggg Ser Lys Ala Leu Leu Asp Ala G31y at c gca I I e A a cac gt g t tt t ac gcc aat Hi s Val Phe Tyr Ala Asn gcg A a gcg A a ccc Pr o tt t Ph e 1 50 c ag O31n agt Ser aat As n ccc Pr o tt g Le u 230 t ca Ser cca Pr o c ag O31n aac As n ac g Thr 310 aag ccc Pr o gt c ValI 120 ct g Le u t cc Ser at t I Ie ag a Ar g t cc Ser 200 cgc Ar g cgc Ar g ct g Le u tt a Le u t at Tyr 280 cca Pr o gt g ValI c ac Hi s ccg Pr o ac g Thr gcg A a gt g ValI ggg O31y 1 70 gcg A a ac g Thr gt t ValI gga O31y g at As p 250 ggg G31y gcc A a g aa O31u c ag O31n t ca Ser cat Hi s ac g Thr gac As p 155 ccg Pr a at c I Ie gcg A a at t I Ie t at Tyr 235 aaa Lys5 gca A a ccc Pr a acc Thr t t g Le u 315 gcc gct A a A a t tt tt a Phe Leu 125 cgt ct g Arg Leu 140 ggt ttt O3 y Phe gat gcg As p A a at c gt g I e Val cgg acc Ar g Thr 205 ggc t cc O3 y Ser 220 gag cag O31u (1n ggg t gc 31y cys gcg ct g A a Leu gct tt g A a Leu 285 acg at g Thr l'b t 300 ggt t ca O3 y Ser ggg G31y 110 g at As p ggc G31y gct A a cgg Ar g ggt (31y 1 90 g at As p cgc Ar g t ac Tyr cga Ar g cgc Ar g 270 tt g Le u g at As p g at As p ggc G31y gag O31u ag g Ar g ggt G31y gcg A a 1 75 ac c Thr ac g Thr gag O31u gcg A a g ac As p 255 tt a Le u ggc G31y c ag O31n gt a ValI ggt G31y cgg Ar g ccc Pr a gcc A a 1 60 tt t Ph e ggt G31y ggt O31y gt t ValI gga O31y 240 at t O31y gct A a at t I Ie tt g Le u 320 gcc A a at c I Ie cat Hi s 145 ac c Thr gt g ValI act Thr ct t Le u cca Pr a 225 at a I Ie tt a Le u at t I Ie gga O31y at g vb t 305 at a I Ie tt t Ph e ag g Ar g 130 gt c ValI g at As p c ac Hi s gcg A a t at Tyr 210 gca A a cca Pr a at c I Ie gt t ValI cga Ar g 290 cgt Ar g g aa O31u tt g Le u 115 gca A a ac g Thr ggc O31y g aa O31u tt g Le u 1 g aa O31u g at As p gag O31u g aa O31u g at As p 275 t ca Ser tt t Ph e at g vb t 1 00 gcg A a ct g Le u tt g Le u ac c Thr g at As p 1 act Thr aat As n t cc Ser gct A a ggt O31y 260 c ag O31n gt g ValI g ac As p at g vb t gag (31u gag O31u aag ag c Ser 1 cga Ar g g at As p c aa O31n aac As n tt a Le u 245 ggc O31y gt g ValI at t I Ie ac c Thr ag a Ar g 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1107 t aaat gt tca caggt at tgt cga <210O> 556 <21 1> 328 <21 2> PmT Page 696 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut am cum <400> 556 let Asp Val Al a Hi s Al a 1 Val Asp Gy Asn Ar g Val Al a SIle Hi s 145 Thr Val Thr Leu Pr o 225 I le Leu SIle Gy Met 305 I le Gy Asp Hi s Gy Gy Tyr Leu 115 Al a Thr Gy G u Leu 195 G u Asp G u G u Asp 275 Ser Phe bet 5 Thr G u G u Al a Cys Asn G u G u Lys Ser 165 Ar g Asp G n Asn Leu 245 Gy Val SIle Thr Ar g 325 Ser Val Val Val 70 Ser Al a Al a Pr o Phe 150 G n Ser Asn Pr o Leu 230 Ser Pr o G n Asn Thr 310 Lys Leu Pr o Val Val Val Lys Asp Gy Tr p 135 Al a Tr p Lys Pr o Ar g 215 Al a Al a Thr Al a Tr p 295 Ser G u Asp Asn Gy 40 Al a Thr Al a Pr o Val 120 Leu Ser SIle Ar g Ser 200 Ar g Ar g Leu Leu Tyr 280 Pr o Val Hi s Leu Pr o Val Leu Leu Leu Phe 105 Asp Val Thr Thr Asp 185 Leu Val Leu Tr p Al a 265 Val G n Ar g Al a 10 Pr o Sy Al a G u Leu 90 Pr o Thr Al a Val Sy 170 Al a Thr Val Sy Asp 250 Gy Al a G u G n Hi s Val Al a Al a Pr o 75 Asp Ser Hi s Thr Asp 155 Pr o l Ie Al a SIle Tyr 235 Lys Al a Pr o Thr Leu 315 Hi s Gy Thr Al a Cys Al a Al a Phe Ar g 140 Gy Asp SIle Ar g Gy 220 G u Gy Al a Al a Thr 300 Gy Val Al a Al a Gy Asn Gy Al a Leu 125 Leu Phe Al a Val Thr 205 Ser G n Cys Leu Leu 285 let Ser Ser Val Pr o Val Hi s SIle Sy 110 Asp Sy Al a Ar g Sy 190 Asp Ar g Tyr Ar g Ar g 270 Leu Asp Asp Asp I e Pr o Ar g Tyr Al a Sy G u Ar g Sy Al a 175 Thr Thr G u Al a Asp 255 Leu Sy G n Val G n Leu Sy Al a Sy Hi s Sy Ar g Pr o Al a 160 Phe Sy Sy Val Sy 240 I e Sy Al a SIe Leu 320 <210> 557 Page 697 seql i st cor r ect ed. t xt <211> 756 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(733) <223> RXA02247 <400> 557 acaagaaacc acgatggatc agattatgcg ttttgacacc ttcagatgta ttgatagaaa t gat gagaaa ggaacact aa acgtccgtga gacagttggg gt c Val at c SIle ggg Gy ggc Gy agc Ser gcc Al a gtt Val gat Asp gaa G u ttg Leu 150 gac Asp gtt Val gct Al a gag G u at g Met t cc Ser gga Gy ct g Leu gca Al a gcc Al a ct g Leu 120 ggc Gy gat Asp acc Thr gt g Val aac Asn 200 ggc Gy at t SI e tct Ser ttc Phe gca Al a gat Asp acc Thr ttt Phe at c SIle tgg Tr p ggc Gy 170 gct Al a ccc Pr o t cc Ser t cc Ser gt c Val act Thr tta Leu 75 ggc Gy tcg Ser gag G u gca Al a ttt Phe 155 gaa G u aag Lys aat Asn gtt Val gcg Al a aat Asn gca Al a t cc Ser cgt Ar g ct g Leu ct g Leu ct c Leu 140 gag G u ct g Leu t ac Tyr gac Asp gca Al a t cc Ser ggt Gy 45 gac Asp acc Thr ct g Leu at c SIle cca Pr o 125 aat Asn gtt Val gcg Al a gt c Val t ac Tyr 205 ggc Gy acc Thr gt g Val ct c Leu ggt Gy ggt Gy aag Lys 110 gct Al a ggc Gy t cc Ser gt a Val gaa G u 190 acc Thr gt g Val 15 gtt Val tgc Cys at g Met agc Ser gga Gy 95 cgc Ar g gat Asp aca Thr ct g Leu ggg Gy 175 cgc Ar g gat Asp gaa G u tta Leu ttg Leu cag G n aaa Lys 80 cac Hi s acc Thr ttg Leu t cc Ser at t SIle 160 gat Asp at g Met ttc Phe at g Met 1 cat Hi s gag Gu aca Thr gaa G u gt c Val at c SIle agc Ser gct Al a ttg Leu 145 ccc Pr o at c SIle at g Met acg Thr ttc aca ggt att Phe Thr G y I e ctg gga gat tcc Leu G y Asp Ser ggt gtg cat ttg Sy Val His Leu gtg gcg tcc ttt Val Al a Ser Phe acc tta gat cgc Thr Leu Asp Arg aac ctt gag cgc Asn Leu G u Arg atg caa ggc cat Met n G y His 100 tca gag aac tgg Ser G u Asn Trp 115 cgc tat gtg gtg Arg Tyr Val Val 130 act gta tcg tct Thr Val Ser Ser acc acc ttg cgc Thr Thr Leu Arg 165 gta aac att gag Val Asn lle u 180 acg cgc ggc gtg Thr Arg Gy Val 195 aga gac Arg Asp 210 Page 698 seqi i st car r ect ed. t xt t aggt tagac aacgt gagtg9 aac <210O> 558 <211> 211 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 558 l'bt Phe Thr (31y Hi s Leu G3 y Asp O31u G31y ValI Hi s Thr Val A a Ser G3 u Thr Leu As p Val As n Leu (31u I Ie 'b t O31n (31y 1 00 Ser Ser G3 u As n 115 Ala Ar g Ty r Val 130 Leu Thr Val Ser 145 Pr o Thr Thr Leu I Ie Val As n I Ie 1 80 l'b t Thr Ar g (31y 1 95 Thr Ar g As p 210 le Ser Le u Ph e Ar g Ar g Hi s Tr p ValI Ser Ar g 1 65 (31u Val G31u G31u Leu I Ie G31y G31y Ser 70 A a ValI As p G31u Le u 150 As p ValI Ar g As p G31u 55 Ser vb t As p ValI Lys5 135 G31y Thr As p vb t Ser 40 G31y Le u A a A a Le u 120 G31y As p Thr ValI O31n 25 li1e Hi s G31y A a Thr 1 05 Ar g Ser As p Hi s I Ie 1 85 G31y 10 I Ie Ser Ph e A a As p 90 Thr Ph e I Ie Tr p G31y 1 70 A a Ser Ser ValI Thr Le u G31y Ser O31u A a Ph e 1 O31u Lys5 ValI A a As n A a Ser Ar g Le u Le u Le u 140 O31u Le u Tyr A a Ser G31y As p Thr Le u I Ie Pr o 125 As n ValI A a ValI Tyr 205 G31y Thr ValI Le u G31y G31y Lys5 110 A a G31y Ser ValI O31u 1 ValI ValI cys Ser G31y Ar g As p Thr Le u G31y 1 Ar g Val Ala G1y Asn Thr Pro Asn Asp 200 Thr Asp Phe <210O> 559 <21 1> 1389 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (1366) <223> RXN02248 <400> 559 gat gt gat cg ct aagt acgt cgaacgcat g at gacgcgcg gcgt ggct gg aaacact ccc aat gact aca ccgat t tcac gagagact ag gt tagacaac gt g agt gaa cat gag 115 Val Ser cOu Hisc3u 1 Page 699 seql i st cor r ect ed. t xt cag gca cac agc caa tta gat tct G n Ala His Ser G n Leu Asp Ser gtt gaa gag gcc gct Al a gaa G u gct Al a gca Al a cag G n ggc Gy ctt Leu gtt Val cac Hi s 150 gca Al a gct Al a ct g Leu ttg Leu ttc Phe 230 gcc Al a gtt Val ggt Sy gac Asp at g Met gat Asp gcc Al a aca Thr gat Asp 120 cca Pr o gaa G u gtt Val gtt Val tct Ser 200 gag Su gct Al a gtt Val gt c Val gcc Al a at c SIle cgt Ar g gat Asp ggc G y at t SIle gaa G u cgt Ar g gct Al a tgc Cys 170 gag Su gag Su cag G n ggt Sy ggc G y 250 tct Ser gt c Val ttt Phe t at Tyr cgt Ar g 75 acc Thr tct Ser gcc Al a gct Al a gt c Val 155 gaa G u ct g Leu cag G n gt g Val tac Tyr 235 gac Asp gag G u gt g Val gcc Al a t cc Ser ctt Leu gct Al a gca Al a gac Asp cgt Ar g 140 gat Asp gt g Val cgc Ar g ct c Leu gaa G u 220 cgt Ar g gt g Val tgc Cys gt g Val gcc Al a 45 tcg Ser gat Asp t ac Tyr aca Thr cgc Ar g 125 gaa G u ttg Leu gt c Val cgc Ar g at t SIle 205 act Thr t cc Ser gca Al a ttg Leu val gt a Val gaa G u gga G y ct g Leu acc Thr gac Asp 110 acg Thr ggt Gy gct Al a agt Ser ttc Phe 190 gag G u gt g Val at c SIle t cc Ser act Thr 270 Su 15 gat Asp tta Leu t ac Tyr cct Pr o gt g Val 95 cgc Ar g gat Asp ggc Gy cgc Ar g gaa G u 175 tgc Cys tgg Tr p ct g Leu at c SIle gac Asp 255 ggt Gy u u Al a gat Asp gcc Al a at c SIle ccg Pr o acc Thr gcc Al a ttc Phe gt c Val gct Al a 160 gag G u gat Asp cgt Ar g cct Pr o gat Asp 240 ggt G y gat Asp gaa G u act Thr tgt Cys at g let gtt Val cac Hi s acc Thr ttg Leu 145 gca Al a gac Asp gag G u cgc Ar g acc Thr 225 ggc G y ggc G y gtt Val at c gct I e Al a gat cgt Asp Arg cca gaa Pro G u gcg cca Ala Pro acc gcg Thr Al a gat gcc Asp Al a act ttg Thr Leu 115 cgt ccc Arg Pro 130 gtg cgc Val Arg ggc ctg G y Leu ccc acc Pro Thr cac gat His Asp 195 aag aat Lys Asn 210 gat ttc Asp Phe acc gag Thr G u gaa aac G u Asn gac Asp gaa G u tta Leu tta Leu cac Hi s aac Asn 100 cgc Ar g gga Gy gct Al a cgc Ar g ggc Gy 180 ctg Leu gaa G u ggc G y ctt Leu gtc Val 260 ttt gga tcc cgg Phe Gy Ser Arg 275 Page 700 seql i st cor r ect ed. t xt cgc Ar g gaa Gu ggc G y 310 ggt Sy gcc Al a cgc Ar g gaa Gu gtt Val 390 gga Gy aac Asn tgc gac Cys Asp 280 gct ggt Al a G y 295 att ggt I e G y gcc gac Al a Asp cgc gaa Arg G u tcg ct c Ser Leu 360 ggc cac G y Hi s 375 cat gaa Hi s G u cat gac His Asp tgt Cys cgg Ar g ct g Leu acc Thr ttt Phe 345 aac Asn ggc Gy gac Asp ct c Leu gga cag cag ctg cac gag tct G y G n G n Leu Hi s G u Ser 285 gga gta gtg gtg tac atg cgt Sy Val Val Val Tyr Mbt Arg 300 ctc gcc aag cta cgc gcc tac Leu Ala Lys Leu Arg Ala Tyr 315 320 gtc gat gcc aac ctc gca ctt Val Asp Ala Asn Leu Ala Leu 330 335 ggc acc agc gcc cag att ctc G y Thr Ser Ala G n Ile Leu 350 ttg atc agc aac aac cca gcc Leu Ile Ser Asn Asn Pro Ala 365 att tcc att gcc agc cga acc Ile Ser Ile Ala Ser Arg Thr 380 aat gtt cga tac ctg aaa acc Asn Val Arg Tyr Leu Lys Thr 395 400 cca gat gtc gca ctg tgg gaa Pro Aso Val Ala Leu Tro G u ttg cgc ctg Leu Arg 290 ggg cat G y Hi s 305 caa ct c G n Leu ggt ct t G y Leu tac gac Tyr Asp aag aag Lys Lys 370 ccc at c Pro Ile 385 aag cgt Lys Arg Leu gag G u cag G n cca Pr o ttg Leu 355 gt g Val cct Pr o gac Asp at c SIe gga G y gat Asp gcc Al a 340 ggt Sy gga G y gtt Val cgc Ar g 979 1027 1075 1123 1171 1219 1267 1315 1363 1389 caa gag cac cca gaa On u His Pro u 420 410 415 taaggagcac aacaatggct aaa <210> 560 <211> 422 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 560 Val Ser u His u G n Ala His Ser 1 5 Ala l e Ala Asp li e Al a Al a y Lys Su Asp Arg u Asn Gu Gy Asp Ile 40 Thr Pro G u Leu Val Al a Phe Met Val 55 Cys Ala Pro Leu Thr Ala Lys Asp Ala 70 Vet Thr Ala His Asn G n Asp Ala Arg Val Asp Ala Asn Thr G y Thr Thr y 100 105 G n Leu Asp 10 Al a Val Val I e Phe Al a Arg Tyr Ser Asp Arg Leu G y Thr Al a 90 I e Ser Al a Page 701 Ser Val Al a Ser Asp Tyr Thr G u Asp Leu Tyr Pr o Val Ar g seqi i st car r ect ed. t xt Hi s Thr Leu Ar g 115 Leu Leu Al a Pro Ou Ala Asp Arg Thr Asp Phe 125 Thr Le u 145 Al a As p O31u Ar g Thr 225 (31y G31y ValI Le u G31y 305 O31n G31y Tyr Pr o 385 O31n Pr o Ar g Le u Thr As p 1 95 As n Ph e G u As n G3y 275 Le u Ou On Pr o Le u 355 ValI Pr o As p Hi s Hi s G31y Pr a 1 65 vb t Lys5 I Ie Thr ValI 245 Le u Ar g O31n Ar g O31u 325 As p ValI Le u Al a vb t 405 O31u ValI Hi s 150 Al a Al a Le u Le u Ph e 230 Al a ValI Ar g O31u O31y 310 O31y Al a Ar g O31u ValI 390 O31y As n ValI 135 Thr O31y Ar g I Ie ValI 215 Lys5 I Ie Ar g cys Al a 295 li1e Al a Ar g Ser O31y 375 Hi s Hi s Le u Al a I Ie Pr a 1 85 li1e Ar g ValI Al a Hi s 265 cys Ar g Le u Thr Ph e 345 As n O31y As p Le u Al a ValI 1 O31u Le u O31n ValI Tyr 235 As p O31u O31n ValI Al a 315 As p Thr I Ie Ser ValI 395 As p O31u Le u ValI Ar g I Ie 205 Thr Ser Al a Le u Le u 285 ValI Le u As n Al a As n 365 Al a Tyr Al a O31y Al a Ser Ph e 1 O31u ValI I Ie Ser Thr 270 Hi s Tyr Ar g Le u O31n 350 As n Ser Le u Le u O31y Ar g O31u 1 cys Tr p Le u I Ie As p 255 O31y O31u Al a Al a 335 li1e Pr a Ar g Tr p 415 <210O> 561 <21 1> 1389 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> Page 702 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(1366) <223> FRXA02248 <400> 561 gatgtgatcg ctaagtacgt cgaacgcatg atgacgcgcg aatgactaca ccgatttcac gagagactag gttagacaac cag G n gct Al a gaa G u gct Al a gca Al a cag G n ggc G y ctt Leu gtt Val cac Hi s 150 gca Al a gct Al a ct g Leu ttg Leu cac Hi s ggt Gy gac Asp at g Met gat Asp gcc Al a aca Thr gat Asp 120 cca Pr o gaa G u gtt Val gtt Val tct Ser 200 gag G u caa G n gcc Al a at c SIle cgt Ar g gat Asp ggc G y at t SIle gaa G u cgt Ar g gct Al a tgc Cys 170 gag G u gag G u cag G n tta Leu gt c Val ttt Phe t at Tyr cgt Ar g 75 acc Thr tct Ser gcc Al a gct Al a gt c Val 155 gaa G u ct g Leu cag G n gt g Val gat Asp gt g Val gcc Al a t cc Ser ctt Leu gct Al a gca Al a gac Asp cgt Ar g 140 gat Asp gt g Val cgc Ar g ct c Leu gaa G0u 220 tct Ser gt g Val gcc Al a 45 tcg Ser gat Asp t ac Tyr aca Thr cgc Ar g 125 gaa G u ttg Leu gt c Val cgc Ar g at t SIle 205 act Thr gtt Val gt a Val gaa G u gga G y ct g Leu acc Thr gac Asp 110 acg Thr ggt G y gct Al a agt Ser ttc Phe 190 gag G u gt g Val gaa G u 15 gat Asp tta Leu t ac Tyr cct Pr o gt g Val 95 cgc Ar g gat Asp ggc G y cgc Ar g gaa G u 175 tgc Cys tgg Tr p ct g Leu gag G u gat Asp gcc Al a at c SIle ccg Pr o 80 acc Thr gcc Al a ttc Phe gt c Val gct Al a 160 gag G u gat Asp cgt Ar g cct Pr o gcgtggctgg aaacactccc gtg agt gaa cat gag 11! Val Ser G u His G u 1 gcc atc gct gac atc 16; Ala lie Ala Asp Ile gaa gat cgt gaa aat 21 Su Asp Arg u Asn act cca gaa tta gtc Thr Pro G u Leu Val tgt gcg cca tta acc Cys Ala Pro Leu Thr atg acc gcg cac aat Met Thr Ala His Asn gtt gat gcc aac acc Val Asp Ala Asn Thr 100 cac act ttg cgc ttg His Thr Leu Arg Leu 115 acc cgt ccc gga cac 49! Thr Arg Pro G y His 130 ttg gtg cgc gct gga 54 Leu Val Arg Ala G y 145 gca ggc ctg cgc cca 59! Ala y Leu Arg Pro 165 gac ccc acc ggc atg 64; Asp Pro Thr y IVet 180 gag cac gat ctg aag 69 G u His Asp Leu Lys 195 cgc aag aat gaa att 73! Arg Lys Asn u lie 210 acc gat ttc ggc acg 78 Thr Asp Phe G y Thr 225 ggc acc gag ctt gtt 83! ttc aag gct gtt ggt tac cgt tcc atc atc gat Page 703 seql i st cor r ect ed. t xt Phe Lys Ala Val Gy Tyr Arg Ser lie lie Asp Gy Thr 230 235 240 G u Leu Val 245 gcc Al a gtt Val cgc Ar g gaa G u ggc Gy 310 ggt Gy gcc Al a cgc Ar g gaa G u gtt Val 390 gga G y aac Asn gtt Val gt c Val gac Asp 280 ggt Gy ggt Gy gac Asp gaa G u ct c Leu 360 cac Hi s gaa G u gac Asp ggc G y 250 tct Ser gga Gy gga Gy ct c Leu gt c Val 330 ggc G y ttg Leu at t SIle aat Asn cca Pr o 410 gac Asp gag G u cag G n gt a Val gcc Al a 315 gat Asp acc Thr at c SIle t cc Ser gtt Val 395 gat Asp gt g Val tgc Cys cag G n gt g Val 300 aag Lys gcc Al a agc Ser agc Ser at t SIle 380 cga Ar g gt c Val gca Al a ttg Leu ct g Leu 285 gt g Val ct a Leu aac Asn gcc Al a aac Asn 365 gcc Al a t ac Tyr gca Al a t cc Ser act Thr 270 cac Hi s t ac Tyr cgc Ar g ct c Leu cag G n 350 aac Asn agc Ser ct g Leu gac Asp 255 ggt Gy gag G u at g Met gcc Al a gca Al a 335 at t SIle cca Pr o cga Ar g aaa Lys ggt G y gat Asp tct Ser cgt Ar g t ac Tyr 320 ctt Leu ct c Leu gcc Al a acc Thr acc Thr 400 ggc G y gtt Val ttg Leu ggg Gy 305 caa G n ggt Gy t ac Tyr aag Lys ccc Pr o 385 aag Lys gaa G u ttt Phe cgc Ar g 290 cat Hi s ct c Leu ctt Leu gac Asp aag Lys 370 at c SIle cgt Ar g gt c Val 260 t cc Ser at c SIle gga Gy gat Asp gcc Al a 340 ggt G y gga G y gtt Val cgc Ar g ct g Leu cgg Ar g cag G n cga Ar g gaa G u 325 gat Asp gt g Val ctt Leu gct Al a at g Met 405 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1389 ctg tgg Leu Trp 415 gaa caa gag cac cca gaa Gu Gn Ou His Pro Gu 420 taaggagcac aacaatggct aaa <210> 562 <211> 422 <212> PRT <213> Corynebacter i um gl <400> 562 Val Ser Ou His Ou Gn 1 5 Ala lie Ala Asp Ile Al a G u Asp Arg G u Asn G u Thr Pro G u Leu Val Al a ut am cum Ala His Ser G n Leu Asp Ser Val G u G u 10 Ala y Lys Ala Val Val Val Val Asp Asp 25 G y Asp lie lie Phe Al a Al a G u Leu Al a 40 Phe IVet Val Arg Tyr Ser Ser y Tyr lie Page 704 seqi i st car r ect ed. t xt Lys Asp A a Asp Leu Asp Leu Pro CYS ValI Hi s Thr Le u 145 A a As p O31u Ar g Thr 225 G31y G31y ValI Le u G31y 305 O31n G31y Tyr Ly s Pr o 385 Ly s Pr o A a A a Le u 115 Pr o Ar g Le u Thr As p 1 95 As n Ph e O31u As n O31y 275 Le u O31u O31n Pr o Le u 355 ValI Pr a As p Thr As n Thr Le u Hi s O31y Pr a 1 65 vb t Ly s I Ie Thr ValI 245 Le u Ar g O31n Ar g O31u 325 As p ValI Le u A a vb t 405 A a 70 (31n G31y Le u ValI Hi s 150 A a A a Le u Le u Ph e 230 A a ValI Ar g O31u G31y 310 G31y A a Ar g O31u ValI 390 G31y As p Thr A a ValI 135 Thr G31y Ar g I Ie ValI 215 Ly s I Ie Ar g cys A a 295 li1e A a Ar g Ser G31y 375 Hi s Hi s A a Thr As p 120 Pr a O31u ValI ValI Ser 200 O31u A a ValI ValI As p 280 G31y G31y As p O31u Le u 360 Hi s O31u As p Ar g G31y 1 Pr a Le u A a I Ie Pr a 1 li1e Ar g ValI A a Hi s 265 cys Ar g Le u Thr Ph e 345 As n G31y As p Le u Ar g 75 Thr Ser A a A a ValI 1 55 O31u Le u O31n ValI Tyr 235 As p O31u O31n ValI A a 315 As p Thr I Ie Ser ValI 395 A a A a As p Ar g 140 As p ValI Ar g Le u O31u 220 Ar g ValI cys O31n ValI 300 Ly s A a Ser Ser I Ie 380 Ar g Tyr Thr Ar g 125 O31u Le u ValI Ar g I Ie 205 Thr Ser A a Le u Le u 285 ValI Le u As n A a As n 365 A a Tyr ValI Ar g As p O31y Ar g O31u 1 cys Tr p Le u I Ie As p 255 O31y O31u A a A a 335 li1e Pr a Ar g Ly s Pr a Thr A a Ph e ValI A a 1 O31u As p Ar g Pr a As p 240 O31y As p Ser Ar g Tyr 320 Le u Le u A a Thr Th r 400 Pr o Asp Val Ala Leu 410 Trp O31u 415 Page 705 seqi i st car r ect ed. t xt On Ou His Pro Ou Asn 420 <210O> 563 <21 1> 600 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn <220> <221 CDS <222> (577) <223> RXN02249 <400> 563 atgttcgata cctgaaaacc aagcgtgacc gcatgggaca tgtgggaaca agagcaccca gaaaactaag gagcacaaca t gacct ccca gat gt cgcac at g gct [vb t Al a tt g Le u gt c ValI gca Al a gt c ValI ac c Th r cca Pr o at t I Ie aac As n gag (31u gca Al a gca Al a g at As p ggc G31y g ac As p tt t Ph e ct t Le u g aa (31u 120 aaa Ly s g aa (31u t gg Tr p gga G31y ct g Le u gt a ValI t ac Ty r act Th r c aa O31n gca Al a ccc Pr o gca Al a gca Al a ct t Le u gcc Al a t gc cys ac c Th r gt g ValI gca Al a 140 g ac As p g aa O31u gca Al a 45 cca Pr o tt g Le u g ac As p cca Pr o g aa O31u 125 at g vb t gcc Al a at c I Ie gga G31y gt c ValI ggc G31y t ct Ser at c I Ie 110 cgc Ar g gt c ValI 1 agc gga tt a Ser 03 y Leu 15 t gc gac cgc Cys Asp Arg gca acg gt g Al a Thr Val gt a gt g caa Val Val O31n t gt gt c gt t Cys Val Val 80 gt c acc gaa Val Thr O31u 95 ggc aac ggt 31y Asn (1y t cc ggt gga Ser 03 y 03 y gct gca ct c Al a Al a Leu 145 aaa Ly s ct g Le u ag c Ser g aa O31u cgt Ar g ggc G31y gt g ValI g aa O31u 130 g at As p aaa gaa gga Lys 03 u 03 y gt c gcc gt a Val Al a Val cac aag cac Hi s Lys Hi s gaa t ac cgc O31u Tyr Ar g ct g gca cgc Leu Ala Arg ggc ggc acc O3 y 03 y Thr ct C acc cgc Leu Thr Arg 1 00 t tg act acc Leu Thr Thr 115 ggc t ct gt a O3 y Ser Val act gcc ct c Thr Al a Leu gt g ct t t ct caa at t cgt gca act gag ggt t agact gt tc t t taaaaggt Val Leu Ser On lie Arg Ala Thr Ou Gy 150 155 tt g <210O> 564 <211> 159 <212> PRT <21 3> Cor ynebact er i urn gI ut anm curn Page 706 seqI i st car r ec <400> 564 I'bt Ala Lys (31u (31y Leu Pro Ala Val G31u Leu 1 5 10 Leu Lys Val Ala Val Val Thr Ala Ar g Tr p As n 25 Ar g Leu Hi s Lys Hi s Ala Val Asp Ala G31y Ar g 40 Val Ser G31u Tyr Ar g Val Ilie G31y Ala L eu G31u 55 G31n G31u Leu Ala Ar g Thr Hi s Asp Ala Val Val 70 Val Ar g G31y G31y Thr Pr o Hi s Phe Asp Tyr Val 90 G31u G31y Leu Thr Ar g Ilie Ala Leu Asp Thr Ser 100 105 G3 y Val Leu Thr Thr Asn Thr G3 u G3 u G3 n A a 115 120 31y a31u a31y Ser Val a31u Asp Lys a31y Ala a31u 130 135 Leu Asp Thr Ala Leu Val Leu Ser a31n I Ie Ar g 145 150 155 <210> 565 <211> 600 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221> CIDS <222> (577) <223> FRXA02249 <400> 565 atgttcgata cctgaaaacc aagcgtgacc gcatgggaca tgtgggaaca agagcaccca gaaaactaag gagcacaaca ted. t xt Pr o Asp A a 03 u A a A a Leu Pro A a Leu Cys As p Thr Pr o Val a31u 125 A a [Vb t 140 A a Thr A a lIe a31y ValI a31y Ser I Ie 110 Ar g ValI a u Ser Cys A a ValI Cys ValI a31y Ser A a a31y t gacct ccca gat gt cgcac atg gct aaa gaa gga tt g Le u gt c ValI gca A a gt c ValI ac c Thr gca A a gca A a g at As p ggC a31y g ac As p g aa a31u t gg Tr p gga a31y ct g Le u gt a ValI ct c Le u aac As n cgt Ar g gaa a31u gt t ValI CCC Pr o gca A a gca A a ct t Le u gcc A a g ac As p g aa a31u gca A a 45 cca Pr o tt g Le u I'b t A a Lys 03 u (31y 1 agc gga t ta aaa gtc gcc gt a Ser a31y Leu Lys Val Ala Val 15 t gc gac cgc ct g cac aag cac Cys Asp Arg Leu Hi s Lys Hi s gca acg gt g agc gaa t ac cgc Ala Thr Val Ser a31u Tyr Ar g gt a gt g caa gaa ct g gca cgc Val Val a31n a31u Leu Ala Ar g tgt gtc gtt cgt ggc ggc acc Cys Val Val Ar g a31y a31y Thr Page 707 75 cca cac ttt gat tac gtg tgc gac t Pro His Phe Asp Tyr Val Cys Asp S att gct ctt gat act tcc acc cca a lie Ala Leu Asp Thr Ser Thr Pro I 105 1 aac acc gaa gag caa gcc gtg gaa c Asn Thr u u n Al a Val u P 120 125 gag gac aaa ggc gca gag gca atg g G u Asp Lys G y Ala G u Ala Vet 135 140 gtg ctt tct caa att cgt gca act g Val Leu Ser 3 n Ile Arg Ala Thr C 150 155 ttg <210> 566 <211> 159 <212> PRT <213> Cor ynebact eri um gl ut am cum Si st cor r ect ed. t xt 80 ct gtc acc gaa ggc ctc acc cgc ier Val Thr 3 u y Leu Thr Arg 95 100 t c ggc aac ggt gtg ttg act acc le y Asn y Val Leu Thr Thr 10 115 gc tcc ggt gga gaa ggc tct gta r g Ser Gy Gy u y Ser Val 130 itc gct gca ctc gat act gcc ctc (al Ala Ala Leu Asp Thr Ala Leu 145 ag ggt tagactgttc tttaaaaggt 3u G y <400> 566 IVet Al a Lys 1 Leu Lys Val Arg Leu Hi s Val Ser G u Sn G u Leu Val Arg y G u G y Leu y Val Leu 115 Gy Gu y 130 Leu Asp Thr 145 G u G y 5 Al a Val Lys Hi s Tyr Arg Al a Ar g y Thr Thr Arg 100 Thr Thr Ser Val Al a Leu Leu Pro Ala Val Thr Val SIle 55 Hi s Hi s Al a Thr Asp 135 Leu Al a Asp 40 y Asp Phe Leu u 120 Lys Ser Ar g 25 Al a Al a Al a Asp Asp 105 u Gy n u 10 Tr p y Leu Val Tyr 90 Thr n Al a SIle Leu Asn Ar g u Val Val Ser Al a u Ar g 155 Pr o Al a Al a Leu Al a Cys Thr Val Al a 140 Al a Asp u Al a Pr o Leu Asp Pr o u 125 Thr Thr Al a I e y Val y Ser SIle 110 Ar g Val u Ser Cys Al a Val Cys Val Sy Ser Al a Sy <210> 567 <211> 702 <212> DNA <213> Corynebacteri um gl utam cum <220> Page 708 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(679) <223> RXA02250 <400> 567 caactgaggg ttagactgtt ctttaaaagg tttgacgcct tgcgcgccac cgctaattag ttctaaggat ttgtgaaatc cggtttaatg cctagcagga ccc gac gga gca Pro Asp G y Ala ggg aag cca aag Sy Lys Pro Lys gca gct ttc gct Ala Ala Phe Ala acc aca aag ttt Thr Thr Lys Phe atg gcc gtt cac Met Al a Val Hi s ggt gca gcg gtc G y Al a Al a Val atc atc ttc tcc Ile Ile Phe Ser 105 cgt gcc aac gaa Arg Ala Asn G u 120 ttc tac cca tgg Phe Tyr Pro Trp 135 agc gtg gca cgt Ser Val Ala Arg 150 gct ttc cag tcc Ala Phe G n Ser ttc cgc gac ctc Phe Arg Asp Leu 185 gt ggct gat c caa acg Thr gt a Val ggc Gy ct g Leu at c SIle acc Thr gtt Val gac Asp gtt Val ttg Leu cgc Ar g 170 aac Asn ct c Leu acc Thr aag Lys 60 at g Met gtt Val gt g Val gtt Val at c SIle 140 ctt Leu gga Gy at c SIle agc Ser aca Thr 45 at c SIle ggt G y gac Asp ttc Phe gag G u 125 t ac Tyr cca Pr o gaa Gu aac Asn gat Asp 30 acc Thr gcg Al a gcc Al a act Thr ttg Leu 110 gt g Val ggc G y gac Asp gat Asp aac Asn 15 gcg Al a aag Lys tgg Tr p gt g Val ct g Leu 95 gga G y cgt Ar g at g Met tt t ttt Phe gt g Val 175 gca Al a gaa Gu cca Pr o gt a Val gt g Val 80 gca Al a ct g Leu aac Asn tct Ser gaa Gu 160 gt g Val gt g Val 1 cat Hi s at t SIle tgg Tr p gcc Al a gac Asp ttc Phe act Thr ttc Phe ttc Phe 145 ttc Phe cgc Ar g aca acc aac gcc Thr Thr Asn Ala tcg ggc gct gtc Ser G y Al a Val cag gaa tac acc G n G u Tyr Thr gag ctg gag gtg G u Leu G u Val gtg gtt gtc atc Val Val Val lle gtc gat ttc acc Val Asp Phe Thr cca gcg ttg ggc Pro Ala Leu y 100 cgc cct cgc gtg Arg Pro Arg Val 115 at c gga act cgt lie G y Thr Arg 130 ccc aag ggc agc Pro Lys G y Ser gtt ccc atg tgg Val Pro Vet Trp 165 gcg gtt gcg acc Ala Val Ala Thr 180 t aat t aagct gaa aac aag tac atg cca gag gac G u Asn Lys Tyr IVet Pro G u Asp 190 <210> 568 <211> 193 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 568 Page 709 seqi i st car r ect ed. t xt Val Thr Thr Asn Ala Pro AspGy Ala Thr Asn Asn 11 1 5 10 e As n As n Al a Hi s I Ie Tr p Al a As p Ph e Thr Ph e Ph e 145 Ph e Ar g As p G31y O31u Le u ValI As p Al a Pr o 115 G31y Ly s Pr o ValI ValI Thr ValI I Ie Thr G31y ValI Ar g Ser Tr p 1 65 Thr G31y Al a Thr vb t 70 G31y I e Ar g Ph e Ser 150 Al a Ph e Ly s Al a Thr Al a Al a I Ie Al a Tyr 135 ValI Ph e Ar g Pr o Ph e 40 Ly s ValI Al a Ph e As n 120 Pr o Al a O31n As p Ly s Al a Ph e Hi s ValI Ser 1 05 G31u Tr p Ar g Ser Le u 1 85 O31n Thr Ly s Ph e Ph e Le u G31y ValI O31u 1 As p As n Ser Thr li1e G31y As p Ph e O31u 125 Tyr Pr o O31u Tyr As p Thr Al a Al a Thr Le u 110 ValI G31y As p As p Nibt 1 Al a Ly s Tr p ValI Le u G31y Ar g vb t Ph e ValI 1 Pr o <210O> 569 <21 1> 1146 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1123) <223> RXA01489 <400> 569 gt cat gggat gt cat tccgg cgggct tgt c gacgat cacg agcat t cat g t ct gt t gagt ct at gccgt a gt ct aaaaca gga ct a gac O3 y Leu Asp ggt gt g tt t G31y Val Phe gcc aag aag Al a Lys Lys agc gt t ccg gct gat ct t Ser Val Pro Al a Asp Leu gat ggg ct c cac cgg ggg Asp G31y Leu Hi s Ar g G31y 30 cag gcc gag gag ct g ggt 03 n Al a 03 u 03 u Leu 03 y 45 caa gga O31n (1y 15 cat caa Hi s 03 n gt g cct Val Pr o Page 71(C agt ccaggt t t aggggcagg gt g gat at t t gg agt Val Asp I Ie Tr p Ser 1 t ca gt a gt c acc at t Ser Val Val Thr I I e agt t ta at c ggc gag Ser Leu I Ie 31y (31u t gt gt c at g gt g acc Cys Val N/t Val Thr seql i st cor r ect ed. t xt ttt gac ccg cat ccg atc gct gtg ttt ttg cca ggt aaa gag cca acc 307 Phe Asp Pro His Pro IIe Ala Val Phe Leu Pro y Lys u Pro Thr 60 cgt ttg gct cct ttg gat tat cgc ctt aat ttg gct gcg gaa tgt ggc 355 Arg Leu Ala Pro Leu Asp Tyr Arg Leu Asn Leu Ala Ala u Cys G y 75 80 gtc gat gct gcg ttg gtt att gat ttc act aaa gaa ctc gca ggt ctg 403 Val Asp Ala Ala Leu Val lie Asp Phe Thr Lys G u Leu Ala Gy Leu 95 100 agc gct gaa gag tat ttc aca acc atg atc gtg gat acg ctg cat gcg 451 Ser Ala G u G u Tyr Phe Thr Thr IVet lie Val Asp Thr Leu His Ala 105 110 115 cgt tca gtt gtg gtg ggg gag aac ttc acc ttc ggt gtc aat ggc gct 499 Arg Ser Val Val Val G y G u Asn Phe Thr Phe Gy Val Asn Gy Ala 120 125 130 ggc act gag tcc acg atg cgg gaa ttg gga caa aag ttt ggc gtg aat 547 Sy Thr G u Ser Thr Iet Arg G u Leu y G n Lys Phe y Val Asn 135 140 145 gtc acg att gct ccg ctg ctg cat gat gat gac cag cgt att tgc tcc 595 Val Thr lie Ala Pro Leu Leu His Asp Asp Asp G n Arg lie Cys Ser 150 155 160 165 acc ttg gtg cgc gat tac ttg gat cag ggc gag gtt gag cgc gcg aac 643 Thr Leu Val Arg Asp Tyr Leu Asp G n y G u Val G u Arg Ala Asn 170 175 180 tgg gcg ctt ggt cga cgc tat gcc gtg cgc ggc gaa gtt gtc cgt ggt 691 Trp Ala Leu y Arg Arg Tyr Ala Val Arg Gy G u Val Val Arg y 185 190 195 gct ggc cgt ggc ggc aaa gaa ttg ggc tat ccc acc gcg aat ctc tac 739 Ala y Arg Gy y Lys G u Leu y Tyr Pro Thr Ala Asn Leu Tyr 200 205 210 ctg ccg acc tct gtg gcg ctg ccc gcc gat ggc gtg tat gca ggc tgg 787 Leu Pro Thr Ser Val Al a Leu Pro Ala Asp y Val Tyr Ala y Trp 215 220 225 ttc acc atc acc gat gac cgc gaa atc gac aag gaa atc tcc cgc gat 835 Phe Thr lie Thr Asp Asp Arg u lie Asp Lys u lie Ser Arg Asp 230 235 240 245 atc gac ggc acc atg gtt cca ggc gtg cgt tac caa act gcc att tcc 883 ie Asp G y Thr IVet Val Pro Gy Val Arg Tyr G n Thr Ala lie Ser 250 255 260 gtg ggc acc aat ccc acc ttc ggc gat gag cga cgc agc gtc gag gca 931 Val y Thr Asn Pro Thr Phe y Asp G u Arg Arg Ser Val G u Ala 265 270 275 ttc atc ctc gac cag gaa gcc gac ctg tac ggt cac cat gtc atg gtg 979 Phe lie Leu Asp G n G u Ala Asp Leu Tyr G y His His Val IVet Val 280 285 290 gaa ttc gtg gga cac ttg cgc gac atg gtc aaa ttc aac ggc gtc gac 1027 G u Phe Val G y Hi s Leu Arg Asp IVet Val Lys Phe Asn G y Val Asp 295 300 305 gag cta cta gac gcc atg gcc cga gat gtc acc aac gcc cgc gac atc 1075 G u Leu Leu Asp Ala Iet Ala Arg Asp Val Thr Asn Ala Arg Asp lie 310 315 320 325 Page 711 seql i st cor r ect ed. txt ctt gcc aaa gac aaa ttg ctt ctc gac gcc gac acc cag ccc agc gct Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp Thr G n Pro Ser Ala 330 335 340 taaggccggt caccggccat caa 1123 1146 <210> 570 <211> 341 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 570 Val Asp II e 1 Ser Val Val Ser Leu II e Cys Val Mvet G y Lys G u Al a Al a G u G u Leu Al a Asp Thr Leu 115 Gy Val Asn 130 Lys Phe G y 145 Sn Arg lie Val G u Arg G u Val Val 195 Thr Al a Asn 210 Val Tyr Al a 225 G u Ile Ser G n Thr Al a Arg Ser Val 275 Trp Ser 5 Thr Ile G y G u Val Thr Pro Thr Cys y G y Leu 100 Hi s Al a G y Al a Val Asn Cys Ser 165 Al a Asn 180 Arg G y Leu Tyr Sy Trp Arg Asp 245 l e Ser 260 G u Al a Leu Val Lys Asp 55 Leu Asp Al a Ser Thr 135 Thr Leu Al a Gy Pr o 215 Thr Asp Gy lie Asp Phe Lys 40 Pr o Al a Al a G u Val 120 Su I e Val Leu Ar g 200 Thr SIle Gy Thr Leu 280 Ser Val 10 Asp y 25 G n Al a His Pr o Pro Leu Al a Leu 90 G u Tyr 105 Val Val Ser Thr Ala Pro Arg Asp 170 G y Arg 185 G y G y Ser Val Thr Asp Thr lVbt 250 Asn Pro 265 Asp n Pro Ala Asp Leu 1n y Leu Hi s G u G u I e Al a Asp Tyr 75 Val lile Phe Thr G y G u Ibet Arg 140 Leu Leu 155 Tyr Leu Arg Tyr Lys G u Al a Leu 220 Asp Arg 235 Val Pr o Thr Phe G u Al a Ar g Leu Val Ar g Asp Thr Asn 125 G u Hi s Asp Al a Leu 205 Pr o G u Gy Gy Asp 285 G y Hi s 0 n G y Val Pro Phe Leu Pro Leu Asn Leu Phe Thr Lys lVbt Ile Val 110 Phe Thr Phe Leu G y G n Asp Asp Asp 160 Gn y u 175 Val Arg y 190 G y Tyr Pro Al a Asp G y Ile Asp Lys 240 Val Arg Tyr 255 Asp u Arg 270 Leu Tyr G y Page 712 seqI i st car r ect ed. t xt Hi s Hi s Val I'bt Val O31u Phe Val G31y Hi s Leu Ar g Asp I'bt Val Lys 290 295 300 Phe Asn G31y ValI Asp O31u Leu Leu Asp Al a I'bt Ala Arg Asp Val Thr 305 310 315 320 Asn Ala Arg Asp Ilie Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp 325 330 335 Thr O31n Pr o Ser Al a 340 <210O> 571 <21 1> 1197 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1174) <223> RXA02135 <400> 571 cgt gt cgcag cgat t tgcga gagggt tgt c t tcgt ggtt g aaaacgt t tt aagaaaacag t cggt t tgaa ggagt tgtt a ct ggt ct gcc act agagt t g at g gt t cca gca gag 11 I'bt Val Pro Al a O31u ct t Le u aag tt g Le u cca Pr o O31y c ag O31n gca Al a c ac Hi s g at As p gcg Al a tt c Ph e c ag O31n cca Pr o gca Al a gct Al a ac c Thr gca Al a 120 g aa O31u gt g ValI g ac As p ggc G31y aac As n aaa Lys5 aac As n ggc G31y ggc G31y gcc Al a gaa (31u t cc Ser t gt Cy s aac As n ggc G31y 75 at t I Ie ac g Thr gac As p at g vb t tt t Ph e ct c Le u tt c Ph e t ca Ser gt g ValI ac a Thr t cc Ser gag (31u ac c Thr 140 ccg Pr o ac c Thr at t I Ie 45 aag Lys5 t cc Ser aac As n gt c ValI cgc Ar g 125 c aa O31n g at As p aag Lys5 t cc Ser at c I Ie gcg Al a ggt O31y cga Ar g 110 gt a ValI g aa (31u cat Hi s 15 cca Pr o gca Al a gt t ValI t ac Tyr O31y 95 ct t Le u t ct Ser c ag O31n aaa Lys5 ccc Pr a t gc cys gt t ValI cca Pr a gcc Al a at t I Ie ag g Ar g gt c ValI 1 at c I Ie gga O31y c ag O31n Ph e t cc Ser gcc Al a g at As p t cc Ser g aa O31u 145 ct g Le u t ct Ser ggc O31y gct Al a t ca Ser at c I Ie ac c Thr t gc cys 130 cgc Ar g gct Al a tt g Le u c ag O31n O31y gt a ValI aac As n t cc Ser 115 gga O31y gca Al a c ag O31n ggc O31y at t I Ie g at As p ag c Ser gt g ValI 1 00 ct c Le u t cc Se r ct g Le u at c ggt aag cgc at t gcg gat caa gaa gt g gac gca ggc gcc gac at t Ile Oy Lys Arg IieAiaAsp On OuVal Asp Aia(y AiaAsp Ilie Page 713 seql i st cor r ect ed. t xt tta Leu ct c Leu ggc Gy cgc Ar g at c SIle 230 at t SIle gt a Val agg Ar g gt a Val t cc Ser 310 at t SIle gt c Val gag G u ccc Pr o gga Gy gga Gy 200 gcc Al a cgg Ar g caa G n acc Thr tgg Tr p 280 ct a Leu ggc Gy gtt Val gga Gy 155 gat tta gga Asp Leu G y 170 ttc acc ctc Phe Thr Leu gac gat gaa Asp Asp G u ttc cgc gcc Phe Ar g Al a 220 atc tct tcc I e Ser Ser 235 gca gt t cga Al a Val Ar g 250 gca gcc ct c Al a Al a Leu atc gca gga i e Al a G y gca ctg gcc Al a Leu Al a 300 ggc tcc ggc G y Ser G y 315 ct g at g aac Leu IVet Asn 330 ct a aac gcc Leu Asn Al a at t SIle gca Al a gcc Al a 205 cgc Ar g cca Pr o cgc Ar g ct a Leu cac Hi s 285 ctt Leu gca Al a gac Asp tct Ser ggc Gy gag G u 190 tgg Tr p gac Asp gac Asp acc Thr gcc Al a 270 cgc Ar g gat Asp gcc Al a at g Met t cc Ser 350 160 acc Thr gtt Val ct c Leu cgc Ar g gca Al a 240 gt g Val aaa Lys acc Thr at c SIle gca Al a 320 aca Thr acc Thr gtt Val aaa Lys caa G n 225 gcc Al a ctt Leu ct g Leu gaa G u ct g Leu 305 ct c Leu ttt Phe acc Thr gt c Val gt c Val 210 gac Asp at g Met ct c Leu gcc Al a cca Pr o 290 gaa G u ccc Pr o t ct Ser gcc Al a gt a Val 195 t cc Ser ccc Pr o gca Al a gac Asp cca Pr o 275 gcg Al a ctt Leu ct g Leu t cc Ser gct Al a 180 ggc Gy gcg Al a at c SIe gca Al a ggc Gy 260 ggt Gy cat Hi s gga Gy gt c Val gcc Al a 340 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1197 gaa gcg ccc gag G u Ala Pro G u caa aac acg G n Asn Thr 355 taactttcta agcgatgtcc ggc <210> 572 <211> 358 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 572 IVet Val Pro Ala G u Leu Phe Ala Arg Val G u Phe Pro Asp His Lys 1 5 10 Ile Leu Ala G n Thr Lys Asp Phe His Asp Ser Leu Thr Lys Pro Pro 25 Sy Ser Leu y Lys Leu u n lie y Cys Phe Ile Ser Ala Cys Page 714 seql i st cor r ect ed. t xt Lys Ile Val Val G n Phe Ser Al a Asp Ser G u 145 Al a Thr Val Lys G n 225 Al a Leu Leu G u Leu 305 Leu Phe Pr o Sn lie Pro Pro G y Asp Hi s y 70 Val Ser Leu G n Asn Val I e Ala 100 Ser Leu Asp Hi s 115 Sy Ser Ile Asp Ala Leu Lys II e 150 Ala Asp Ile Leu 165 Ala Ala Ala Leu 180 Val Gy Arg y 195 Ser Ala lie Arg Pro Ile Ala lle 230 Ala Ala Phe II e 245 Asp y Val Val 260 Pro G y Ala Ar g 275 Ala His Ser Val Leu Gy IVet Ser 310 Leu Val Lys Ile 325 Ser Al a y Val 340 G n Asn Thr G u 355 Ar g Val Met Ar g G u Val 135 Gy SIle Val Thr Asp 215 Al a Al a Val Ar g Al a 295 Leu Al a Asp Pro Leu Asn Asn Al a Al a Thr Al a 120 G u Lys Pr o Gy Gy 200 Al a Ar g G n Thr Tr p 280 Leu Gy Val Thr G u Thr 105 Tr p Asp Ar g Gy Thr 185 I le et Lys Al a Al a 265 Phe Asn G u Asp Lys Asn 90 Gy Gy Al a SIle Asp 170 Phe Asp Phe SIle Al a 250 Al a SIle Al a Gy Leu 330 Gy 75 I le Thr Asp Met Al a 155 Leu Thr Asp Ar g Ser 235 Val Al a Al a Leu Ser 315 Met Ser Val Thr Ser G u Thr 140 Asp Gy Leu G u Al a 220 Ser Ar g Leu Gy Al a 300 Gy Asn Ser Asn Val Ar g 125 G n G n SIle Al a Al a 205 Ar g Pr o Ar g Leu Hi s 285 Leu Al a Asp Al a Sy Ar g 110 Val G u G u Sy G u 190 Tr p Asp Asp Thr Al a 270 Ar g Asp Al a Met Ty r Sy Leu Ser G n Val Asn 175 Pr o Lys Leu Leu Pr o 255 Asn Ser Pr o Thr Ser 335 Pr o Al a SIe Ar g Val Asp 160 Thr Val Leu Ar g Al a 240 Val Lys Thr SIe Al a 320 Thr Sy Pro Leu Asn Ala Ser Ser u Ala 345 350 <210> 573 <211> 1146 <212> DNA <213> Corynebacter i um gl utam cum Page 715 seql i st cor r ect ed. t xt <220> <221> CDS <222> (101)..(1123) <223> RXA01489 <400> 573 gtcatgggat gtcattccgg cgggcttgtc gacgatcacg agcattcatg tctgttgagt ctatgccgta gtctaaaaca agtccaggtt taggggcagg gga Gy ggt Gy gcc Al a ttt Phe cgt Ar g gt c Val agc Ser cgt Ar g ggc Gy gt c Val 150 acc Thr tgg Tr p gct Al a ct g Leu gac Asp ttt Phe aag Lys ccg Pr o gct Al a gct Al a gaa G u gtt Val 120 gag G u at t SIle gt g Val ctt Leu cgt Ar g 200 acc Thr gtt Val ggg Gy gcc Al a ccg Pr o ttg Leu ttg Leu t at Tyr gt g Val acg Thr ccg Pr o gat Asp 170 cga Ar g ggc Gy gt g Val ccg Pr o ct c Leu gag G u at c SIle gat Asp 75 gtt Val tt c Phe ggg Gy at g Met ct g Leu 155 tac Tyr cgc Ar g aaa Lys gcg Al a gct Al a cac Hi s gag G u gct Al a t at Tyr at t SIle aca Thr gag G u cgg Ar g 140 ct g Leu ttg Leu t at Tyr gaa G u ct g Leu 220 gat Asp cgg Ar g ct g Leu 45 gt g Val cgc Ar g gat Asp acc Thr aac Asn 125 gaa G u cat Hi s gat Asp gcc Al a ttg Leu 205 ccc Pr o ctt Leu ggg Gy ggt Gy ttt Phe ctt Leu ttc Phe at g Met 110 ttc Phe ttg Leu gat Asp cag G n gt g Val 190 ggc Gy gcc Al a caa G n 15 cat Hi s gt g Val ttg Leu aat Asn act Thr 95 at c SIle acc Thr gga Gy gat Asp ggc Gy 175 cgc Ar g t at Tyr gat Asp gga Gy caa G n cct Pr o cca Pr o ttg Leu 80 aaa Lys gt g Val ttc Phe caa G n gac Asp 160 gag G u ggc G y ccc Pr o ggc Gy gt g Val 1 t ca Ser agt Ser tgt Cys ggt Gy gct Al a gaa G u gat Asp ggt Sy aag Lys 145 cag G n gtt Val gaa G u acc Thr gt a Val tta Leu gt c Val aaa Lys gcg Al a ct c Leu acg Thr gt c Val 130 ttt Phe cgt Ar g gag G u gtt Val gcg Al a 210 gt c Val at c SIle at g let gag Gu gaa G u gca Al a ct g Leu 115 aat Asn ggc Sy at t SIle cgc Ar g gt c Val 195 aat Asn acc Thr ggc G y gt g Val cca Pr o tgt Cys ggt Sy 100 cat Hi s ggc Sy gt g Val tgc Cys gcg Al a 180 cgt Ar g ct c Leu gat att tgg Asp lie Trp agt Ser att SIe gag G u acc Thr acc Thr ggc Sy ctg Leu gcg Al a gct Al a aat Asn t cc Ser 165 aac Asn ggt G y t ac Tyr gtg tat gca ggc tgg Val Tyr Ala y Trp 225 Page 716 seql i st cor r ect ed. t xt ttc acc atc acc gat gac cgc gaa atc gac aag gaa atc Phe Thr Ile Thr Asp Asp Arg Gu lie Asp Lys Gu Ile 230 235 240 atc gac ggc acc atg gtt cca ggc gtg cgt tac caa act I e Asp G y Thr ebt Val Pro G y Val Arg Tyr G n Thr 250 255 gtg ggc acc aat ccc acc ttc ggc gat gag cga cgc agc Val y Thr Asn Pro Thr Phe y Asp G u Arg Arg Ser 265 270 ttc atc ctc gac cag gaa gcc gac ctg tac ggt cac cat Phe I e Leu Asp G n G u Al a Asp Leu Tyr G y Hi s Hi s 280 285 290 gaa ttc gtg gga cac ttg cgc gac atg gtc aaa ttc aac G u Phe Val G y Hi s Leu Arg Asp IVet Val Lys Phe Asn 295 300 305 gag cta cta gac gcc atg gcc cga gat gtc acc aac gcc G u Leu Leu Asp Ala IVet Ala Arg Asp Val Thr Asn Ala 310 315 320 ctt gcc aaa gac aaa ttg ctt ctc gac gcc gac acc cag Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp Thr G n 330 335 taaggccggt caccggccat caa <210> 574 <211> 341 <212> PRT <213> Cor ynebact eri um gl ut ai cum t cc Ser gcc Al a gt c Val 275 gt c Val ggc Gy cgc Ar g ccc Pr o cgc Ar g at t l Ie 260 gag Gu at g Met gt c Val gac Asp agc Ser 340 gat Asp 245 t cc Ser gca Al a gt g Val gac Asp at c SIe 325 gct Al a 835 883 931 979 1027 1075 1123 1146 <400> 574 Val Asp II 1 Ser Val V, Ser Leu II Cys Val A G y Lys G Al a Al a G G u Leu Al Asp Thr LE 11 Sy Val A, 130 Lys Phe G 145 e Trp Ser 5 al Thr Il e e G y G u bt Val Thr u Pro Thr u Cys y a G y Leu 100 eu Hi s Al a 15 sn G y Al a y Val Asn Leu Val Lys Asp Leu Asp Al a Ser Thr 135 Thr Asp Phe Lys 40 Pr o Al a Al a G u Val 120 Su lie Ser Val 10 Asp y G n Al a His Pr o Pro Leu Al a Leu 90 G u Tyr 105 Val Val Ser Thr Ala Pro Pro Ala Asp Leu 1n y Leu G u SIle Asp Val Phe Sy Met Leu 155 Ar g Leu Val Ar g Asp Thr Asn 125 G u Hi s Hi s Val Leu Asn Thr I e Thr Sy Asp G n Arg lie Cys Ser Thr Leu Val Arg Asp Tyr Leu Asp G n y G u Page 717 seql i st cor r ect ed. t xt 165 170 175 Val u Arg Ala Asn Trp Ala Leu Gy Arg Arg Tyr Ala Val Arg y 180 185 190 G u Val Val Arg G y Al a y Arg G y G y Lys G u Leu G y Tyr Pro 195 200 205 Thr Ala Asn Leu Tyr Leu Pro Thr Ser Val Ala Leu Pro Ala Asp y 210 215 220 Val Tyr Ala y Trp Phe Thr Ile Thr Asp Asp Arg G u lie Asp Lys 225 230 235 240 G u Ile Ser Arg Asp Ile Asp G y Thr IVet Val Pro G y Val Arg Tyr 245 250 255 G n Thr Ala Ile Ser Val y Thr Asn Pro Thr Phe y Asp G u Arg 260 265 270 Arg Ser Val G u Ala Phe Ile Leu Asp n G u Ala Asp Leu Tyr y 275 280 285 His His Val bet Val G u Phe Val G y Hi s Leu Arg Asp bet Val Lys 290 295 300 Phe Asn G y Val Asp G u Leu Leu Asp Al a IVet Al a Arg Asp Val Thr 305 310 315 320 Asn Ala Arg Asp Ile Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp 325 330 335 Thr G n Pro Ser Ala 340 <210> 575 <211> 805 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (87)..(782) <223> RXN01712 <400> 575 gccggct t ag gctcctccga ccagaacccc actggcacct ttggataaca ccat t ccgtg taaacaagct ggcctagact tgaaac atg gtc gat atc ttg gaa ctc atc ggt 113 IVet Val Asp Ile Leu u Leu Ile Gy 1 ccc cta cct ttt gtg tct acg cca gag tta agg gca att gtc gtg act 161 Pro Leu Pro Phe Val Ser Thr Pro G u Leu Arg Ala lie Val Val Thr 15 20 gcc att aat ggt tcc acc act att aat ggc acg tct ggt cag ctt gga 209 Ala lie Asn y Ser Thr Thr Ile Asn y Thr Ser Gy n Leu y 35 aat tcc acg gat acg gaa ctt ctg ttg gcg ctt cgc agg tgg tcg gac 257 Asn Ser Thr Asp Thr G u Leu Leu Leu Ala Leu Arg Arg Trp Ser Asp 50 gtg gtg ttg gtt ggg tcg agc acg gtg aag gct gaa aat tat ggt ggc 305 Val Val Leu Val G y Ser Ser Thr Val Lys Ala G u Asn Tyr G y G y 65 Page 718 seql i st cor r ect ed. t xt gtg gag Val G u gtt tcg cct gaa Val Ser Pro G u atc cag aag caa cgc cag gaa gcg at t G u Al a l I e gtg gat act Val Asp Thr acg gat aat Thr Asp Asn gct cag gtt Al a n Val 140 aag ctt agg Lys Leu Arg 155 acg ttg tat Thr Leu Tyr 170 cac acg att His Thr Ile aag ggc ggc Lys G y G y gtc gat gat Val Asp Asp 220 ccg Pr o ttt Phe 110 gat Asp gag G u ttg Leu cag G n ccg Pr o 190 gat Asp agc Ser at t I e 95 tt c Phe caa G n gt g Val ggt G y at g Met 175 acg Thr gcg Al a acc Thr I e gcg Al a ctt Leu gca Al a gag G u t ac Tyr 160 ttg Leu ttg Leu ccg Pr o ct a Leu gt g Val gag G u aag Lys acg Thr 145 gcc Al a gcc Al a tcg Ser cgc Ar g Sn Lys Gn Arg n at g Met gcc Al a cag G n 130 ttg Leu cgc Ar g gcc Al a ggc G y cga Ar g 210 t ca Ser gaa G u 115 cag G n acg Thr at t SIle gat Asp agc Ser 195 ttc Phe ggg a y 100 gt g Val cgg Ar g gcg Al a gat Asp ctt Leu 180 gt g Val gcg Al a gag ttg G u Leu ttg aat Leu Asn ccg at c Pro Ile gtg gat Val Asp 135 gtt ggc Val G y 150 gag ggc Su G y gat gtg Asp Val cgc ccc Arg Pro gag cac G u Hi s 215 ggt G y ttt Phe at c SIle 120 gct Al a gt c Val ggt G y tgg Tr p acg Thr 200 gt c Val cag G n gat Asp 105 at c SIe ggg G y gaa G u gca Al a cat Hi s 185 gtg Val ttt Phe ttc ttg cgg tat aag Phe Leu Arg Tyr Lys 225 cgc gcc aag Arg Ala Lys 230 t gagt gt t gg act ct ccgga t ct <210> 576 <211> 232 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 576 IVet Val Asp Ile Leu u Leu Ile y 1 5 Pro u Leu Arg Ala lie Val Val Thr I le Asn G y Thr Ser G y G n Leu G y 40 Leu Leu Ala Leu Arg Arg Trp Ser Asp Thr Val Lys Al a u Asn Tyr G y G y 70 G n Lys n Arg n G u Leu G y G n Pro Leu I 10 Al a I le Asn Ser Val Val I Val Gu G u Al a 90 Page 719 Phe Sy Asp Val Ser Pr o Ser Thr G u Ser G u I e seql i st cor r ec Val INet Ser G y Ser Leu Asn Phe Asp Val Asp 100 105 Su Ala Gu Val Pro Pro Ile Ile Ile Thr Asp 115 120 Lys n G n Arg Leu Val Asp Ala y Ala G n 130 135 Thr Leu Thr Ala G u Val y Val G u Lys Leu 145 150 155 Ala Arg lie Asp Cys u y y Ala Thr Leu 165 170 Ala Ala Asp Leu Val Asp Val Trp His His Thr 180 185 Ser y Ser Val G u Arg Pro Thr Val Lys y 195 200 Arg Arg Phe Ala Leu G u His Val Phe Val Asp 210 215 Phe Leu Arg Tyr Lys Arg Ala Lys 225 230 <210> 577 <211> 578 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (55)..(555) <223> FRXA01712 <400> 577 cttctgttgg cgcttcgcag gtggtcggac gtggtgttgg ted. txt Thr Arg Asn Ser 125 Val lile 140 Arg Ser Tyr G y Ile Asp Sy Asp 205 Asp Asp 220 Phe G n Val Sy Met 175 Thr Al a Thr ttgggtcgag cacg aag Lys caa G n t ca Ser gaa G u cag G n acg Thr at t gaa G u cag G n tcg Ser ccg Pr o ctt Leu gag Stu tgt t at Tyr ttg Leu aat Asn at c SIle gat Asp ggc gcy ggc ggt G y ggt G y ttt Phe at c SIle 55 gct Al a gt c Val ggt ggc G y cag G n gat Asp at c SIle ggg G y gaa gau gca gt g Val gaa G u 25 gt g Val acg Thr gct Al a aag Lys acg gag G u gcg Al a gat Asp gat Asp cag G n ctt Leu ttg gtt tcg Val Ser I att ccg lie Pro I act cgc 1 Thr Arg I aat t cc Asn Ser 60 gtt att Val lie 75 agg tct 1 Arg Ser I tat ggg Page 720 gaa G u at t I e ttc Phe caa G n gt g Val ggt t y at g at c I e gcg Al a ctt Leu gca Al a gag G u t ac Tyr ttg cag G n gt g Val gag G u aag Lys acg Thr gcc Al a gcc gtg Val 1 aag Lys at g let gcc Al a cag G n ttg Leu cgc Ar g gcc I Ie g at As p ag c Ser 130 tt c Ph e cgg Ar g cys 1 00 gt t ValI gag O31u tt g Le u aag Ly s G31y gt g ValI ccc Pr o c ac Hi s 1 50 gcc Al a seqi i st car r ect ed. t xt Al a Thr Leu Tyr 31y O31n l'b t Leu Al a Al a 105 110 cat cac acg at t gat ccg acg t tg t cg ggc Hi s Hi s Thr I Ie As p Pr o Thr Leu Ser G31y 120 125 gt g aag ggc ggc gat gat gcg ccg cgc cga Val Lys G31y G31y Asp Asp Al a Pr o Ar g Ar g 140 145 t tt gt c gat gat gac agc acc ct a t tc tt g Phe Val Asp Asp Asp Ser Thr Leu Phe Leu 155 160 t gagt gt t gg act ct ccgga t ct <210O> 578 <211> 167 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 578 Val Lys Al a Lys O31n Ar g l'b t Ser G31y Al a 03 u Val O31n O31n Ar g Leu Thr Al a Ar g I Ie As p Al a Asp Leu 115 O3 y Ser Val 130 Ar g Phe Al a 145 Leu Arg Tyr 31u As n O31n (1u Ser Leu Pr o Pr o Leu Val O3 u Val Cys (1u 1 00 Val As p O31u Ar g Leu 03 u Lys Ar g 1 Tyr 0 y 0 yVal O31y Ph e I Ie Al a ValI O31y Tr p Thr 135 ValI Ly s O31u 25 ValI Thr Al a Ly s Thr 1 05 Hi s Ly s ValI O31u 10 Al a As p As p O31n Le u 90 Le u Thr O31y As p ValI I Ie Thr As n ValI 75 Ar g Tyr I Ie O31y As p 1 55 Pr o Pr o Ph e As p O31u Le u O31n Pr a 125 As p Ser Ou le Ph e O31n ValI O31y vb t 110 Thr Al a Thr lIe Al a Le u Al a O31u Tyr Le u Le u Pr a Le u O31n ValI O31u Ly s Thr Al a Al a Ser Ar g Ph e 1 <210O> 579 <21 1> 831 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (808) Page 721 seql i st cor r ect ed. t xt <223> RXN02384 <400> 579 ctgatgaggc ggatatccgc aacatcgaca gcattgatga aatacgagcc tggctacgag gacgattaag aggtagtcct actcccacct ttgccagctg gtg act cgt cgc Val Thr Arg Arg at t SIle cag G n gcc Al a agc Ser ct g Leu ttg Leu cca Pr o ggc Gy gac Asp at c SIle 150 gac Asp tgt Cys aac Asn cat Hi s tgg Tr p 230 ct c Leu caa G n gca Al a gat Asp gac Asp gag G u gcg Al a gaa G u 120 ttg Leu gct Al a gcg Al a gca Al a gaa G u 200 gag G u cac Hi s gac Asp t ca Ser t cc Ser gag G u cag G n gct Al a cgc Ar g gt g Val ggt Sy gat Asp 170 ttg Leu gac Asp aac Asn ggg G y aca Thr gt g Val cgc Ar g gt g Val 75 gcc Al a caa G n gt g Val tcg Ser ggc G y 155 cag G n acc Thr ct c Leu aat Asn cag G n gag G u ct g Leu gcc Al a cgc Ar g aaa Lys tgg Tr p gat Asp ctt Leu 140 acg Thr t ac Tyr gcc Al a aag Lys gt g Val 220 gaa tac aac G u Tyr Asn 15 tct gac ct g Ser Asp Leu caa aaa aac G n Lys Asn aac acc gca Asn Thr Al a gat aag cgt Asp Lys Arg 80 cac act gag Hi s Thr G u 95 cac gat ccg His Asp Pro 110 gcg cgc cgg Al a Ar g Ar g gat tgg gat Asp Trp Asp aat gcg ct g Asn Ala Leu 160 atg ttc tct IVet Phe Ser 175 cct cgc t at Pro Arg Tyr 190 tct tcg gcg Ser Ser Al a aac gcc cag Asn Al a G n 1 gcc Al a ggc G y at c SIle agc Ser ctt Leu gt g Val cag G n gca Al a gaa 145 acc Thr gga G y t at Tyr gtt Val tgg Tr p 225 acg t cc cga Thr Ser Arg ttt caa cag Phe G n G n acc cat gt g Thr Hi s Val gcg gtt gcg Al a Val Al a cgg gaa acg Arg G u Thr gat t cc gaa Asp Ser G u 100 tgg gca cca Tr p Al a Pr o 115 cgc caa gtt Arg n Val 130 ggc acc gtg G y Thr Val tcg aat ct t Ser Asn Leu ctt gga aat Leu G y Asn 180 gca ggt agt Al a G y Ser 195 t cc aac agc Ser Asn Ser 210 tat ctt gac Tyr Leu Asp ctg Leu at g let gcg Al a ttc Phe gcg Al a cat Hi s t at Tyr ccc Pr o gtc Val ct c Leu ttg Leu 165 acc Thr gag G u cct Pr o ggc G y aac atg ggt gttt Asn Vbt G y Val acg cag Thr G n 235 taaagaagat ggcaataaaa atg 831 Page 722 seql i st cor r ect ed. t xt <210> 580 <211> 236 <212> PRT <213> Corynebacteri um gl ut am cum <400> 580 Val 1 Al a Gy SIle Ser Leu Val G n Al a G u 145 Thr Gy Tyr Val Tr p 225 Thr Arg Ar g Thr Ser Ar g Phe G n G n Thr Hi s Val Al a Val Al a Arg G u Thr Asp Ser G u 100 Tr p Al a Pr o 115 Arg n Val 130 G y Thr Val Ser Asn Leu Leu G y Asn 180 Al a G y Ser 195 Ser Asn Ser 210 Tvr Leu AsD Leu 5 Mbet Al a Phe Al a Hi s Tyr Pr o Val Leu Leu 165 Thr G u Pr o Sy Ile Leu Leu Arg G n Al a Ser Leu 70 Leu Pr o Sy Asp SIle 150 Asp Cys Asn Hi s Tr p 230 Sy Ser Ser 55 I le Sy Sy Sy G u 135 Val Leu Tr p Pr o Phe 215 Asn G n Al a 40 Asp Asp G u Al a G u 120 Leu Al a Al a Al a G u 200 G u ?bt Leu Al a Leu Al a Tr p Ar g 105 Ser bet Hi s Tyr G n 185 Asp Gy Gy Gy Thr Val Ar g Val Al a G n Val Ser Sy 155 G n Thr Leu Asn Thr 235 G n G u Leu Al a Ar g Lys Tr p Asp Leu 140 Thr Tyr Al a Lys Val 220 G n Thr Leu Val Phe Val Thr Ar g Val 125 Asp SIle Pr o Ar g SIle 205 G u Tyr Asp Lys Thr Lys Thr Asp Ar g Tr p Al a Phe 175 Ar g Ser Al a <210> 581 <211> 453 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(430) <223> RXN01560 <400> 581 atgggagcaa ggctcattta gctacttcga cgtggaagcg cacatcgttg agttgattcc tgcatcagta agcgatgcgt tttaggcaca tctgcgatcc gtg ggc gtg tcc tac 115 Page 723 seql i st cor r ect ed. t xt Val G y Val Ser Tyr 1 atc atc gcc ggc gat gag cag ctg gat atg gca gaa gcc gtt cgc aaa 163 lie lie Ala Gy Asp G u n Leu Asp IVet Al a G u Ala Val Arg Lys 15 att ggg gag acc ttt aaa act gag gaa att atc ctt ggt ggc gga gga 211 lie Gy u Thr Phe Lys Thr Gu u Ile lie Leu Gy Gy Gy y 30 acc ctg aac tgg tcc atg ctc cgc gac ggt ttg tgc gac gag gtt agc 259 Thr Leu Asn Trp Ser IVet Leu Arg Asp G y Leu Cys Asp G u Val Ser 45 atc gtg atg atg cca atc gcc gat ggt gaa aag cac acc cac tct ttg 307 lie Val IVet IVet Pro lie Ala Asp G y G u Lys His Thr His Ser Leu 60 ttc gaa gcc gat gaa aaa tac tca gca ccg ttg ccg atc ggt ttt tca 355 Phe G u Ala Asp G u Lys Tyr Ser Ala Pro Leu Pro lie y Phe Ser 75 80 ctc gcc agc gtt gaa cca cta gaa gat gga agc gtt tgg atg cgt tac 403 Leu Ala Ser Val G u Pro Leu G u Asp y Ser Val Trp IVet Arg Tyr 95 100 ggg gtc aat ggc cca gtg gac gcg aac taggtagcaa at actcgctc 450 G y Val Asn Gy Pro Val Asp Al a Asn 105 110 ttt 453 <210> 582 <211> 110 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 582 Val y Val Ser Tyr Ile Ile Ala Gy Asp Gu Gn Leu Asp IVet Ala 1 5 10 Su Ala Val Arg Lys lie Gy Gu Thr Phe Lys Thr u u lu e lie 25 Leu Gy Gy Gy Gy Thr Leu Asn Trp Ser IVet Leu Arg Asp y Leu 40 Cys Asp u Val Ser Ile Val Vebt Vet Pro I e Ala Asp Gy Gu Lys 55 His Thr His Ser Leu Phe G u Ala Asp G u Lys Tyr Ser Ala Pro Leu 70 75 Pro lie y Phe Ser Leu Ala Ser Val G u Pro Leu G u Asp y Ser 90 Val Trp IVet Arg Tyr G y Val Asn G y Pro Val Asp Al a Asn 100 105 110 <210> 583 <211> 1137 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> Page 724 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(1114) <223> RXN00667 <400> 583 ggcatcagtg tttgaaggga aaagcaggtc aaacaaggtg agcaccgaga tctgtgctga agaattcaat gattgggttg cggctgattt gagcgatcac t cc Ser ccc Pr o gcg Al a cag G n ttc Phe gac Asp gcc Al a gat Asp cgt Ar g act Thr 150 t cc Ser caa G n gca Al a t cc Ser tct Ser cgc Ar g ccg Pr o cac Hi s gcc Al a at g Met gaa G u at c SIle 120 cgc Ar g aac Asn gt g Val ttg Leu ttc Phe 200 at c SIle agg Ar g ggc Gy cgc Ar g aat Asn ctt Leu cag G n ccg Pr o gt c Val at c SIle gat Asp gt c Val 170 gcc Al a cca Pr o aaa Lys gt a Val ct c Leu gcc Al a ccg Pr o gtt Val 75 ccc Pr o at c SIle at t SIle acc Thr ct c Leu 155 at g bet cga Ar g ggg gay gaa G u gt a Val aac Asn at c l Ie gcc Al a gt c Val gac Asp cct Pr o gac Asp gaa G u 140 acc Thr gt g Val cgt Ar g ct a Leu cgc Ar g 220 caa G n cct Pr o gat Asp 45 gac Asp aac Asn gac Asp t ac Tyr aag Lys 125 acc Thr cca Pr o aaa Lys gag G u ctt Leu 205 ggc G y aag Lys t cc Ser ttt Phe aac Asn cac Hi s gac Asp aaa Lys 110 cca Pr o gct Al a gct Al a aaa Lys gcg Al a 190 gat Asp at c SIle cca Pr o 15 cgt Ar g gtt Val gcc Al a t ac Tyr at t SIle 95 at t SIle ccc Pr o ct g Leu cac Hi s cca Pr o 175 t cc Ser gat Asp gt g Val aaa Lys gt g Val gaa G u gaa G u ggc G y 80 tgg Tr p cat Hi s t ac Tyr gt g Val cgc Ar g 160 gaa G u aaa Lys ggt G y caa G n atg att Vet Ile 1 atg aaa Vet Lys cgc ttg Arg Leu tac ct c Tyr Leu gcg ctt Al a Leu gag ccc G u Pro ttc tac Phe Tyr gtc att Val lile ct a gca Leu Al a 130 aaa at g Lys IVet 145 ct c gat Leu Asp ct c cgt Leu Arg acc t at Thr Tyr ccc gcg Pr o Al a 210 gcc ttc Al a Phe 225 gc Al gc Al cc Pr 3 at I I ca G ta Ty cg Ar ca Hi 11 ac Th cg Ar cg Ar gg G ga 19 at I I gt Va g ttg aag a Leu Lys g ccg cta a Pro Leu g ctc gac o Leu Asp t tcc acg e Ser Thr a gcg cgt n Al a Ar g c gcc ccc r Ala Pro c atg ccc g IVet Pro 100 c gaa gac s G u Asp c at g cct r IVet Pro t gtg ctg g Val Leu c ct g act g Leu Thr 165 c gct tac y Al a Tyr 180 g gca at c u Ala lle t tgg gaa e Trp u c gtg gaa I Val G u ggc ccc gtc aac gca cgc act gaa ctg gtg tca gtc acc cca gtc gaa Page 725 seql i st cor r ect ed. t xt Gy Pro Val Asn Ala Arg Thr G u Leu Val Ser Val Thr 230 235 240 gac gcc gaa cag agc at ct c gaa gaa atg cac ggg cca Asp Ala G u G n Ser IIe Leu u u Iet His y Pro 250 255 caa gcg cgc tac gtt cta gcc ccc tca aca ggc aaa acc G n Ala Arg Tyr Val Leu Ala Pro Ser Thr y Lys Thr 265 270 cgc atc cac atg cgc gac ttc gca gcc ccc atc ctc ggc Arg lie His IVet Arg Asp Phe Ala Ala Pro lie Leu G y 280 285 290 tac ccc gtc ctc cac gca gtc gac gat gag gac tac acc Tyr Pro Val Leu His Al a Val Asp Asp G u Asp Tyr Thr 295 300 305 cac ctc atc gcc cgc acg cta acc ttc gtg gat cct caa His Leu lie Ala Arg Thr Leu Thr Phe Val Asp Pro G n 310 315 320 gaa cgt acc ttt gtg agt aat cga cct acg gga agt ttg G u Arg Thr Phe Val Ser Asn Arg Pro Thr y Ser Leu 330 335 agacatcacc cag <210> 584 <211> 338 <212> PRT <213> Cor ynebact eri um gl ut arri cum Pro Val u 245 ctc ccc cgc Leu Pro Arg 260 cac cag ct g Hi s G n Leu 275 gac ccc ct c Asp Pro Leu acc cca at g Thr Pro IVet acc aac gag Thr Asn G u 325 t aggcct cgt 883 931 979 1027 1075 1124 1137 <400> 584 'e t Ile Al 1 IVet Lys Al Arg Leu Pr Tyr Leu II Al a Leu G G u Pro T} Phe Tyr Ar Val Ile Hi 11 Leu Al a T\ 130 Lys IVet Ar 145 Leu Asp Ar a Leu a Pro o Leu e Ser n Al a yr Al a rg Mbet 100 s u 15 hr IVet rg Val g Leu Lys 5 Leu Asp Thr Ar g Pr o Pr o Asp Pr o Leu Thr 165 Met SIle Al a Ar g Asp Thr Al a Asp Gy 135 Gy Gy Asn Arg Asp y 25 li e Ar g Arg Asn Asp Leu Val G n Arg Pro 105 Leu Val His Il e Asn Asp Leu Val 170 Val Leu Al a Pr o Val Pr o SIle SIle Thr Leu 155 G n Pr o Asp Asp Asn Asp Tyr Lys 125 Thr Pr o Lys Ser Phe Asn Hi s Asp Lys 110 Pr o Al a Al a Iet Val Lys Lys Pro G u 175 Page 726 Leu Thr Pr o Al a 225 Val Gy Lys Leu Tyr 305 Pr o Ser Gy G u 195 I le Val Pr o Leu Hi s 275 Asp Thr Thr Tyr SIle G u G u G u 245 Ar g Leu Leu Met G u 325 G n Al a Ser Gy 230 Asp G n Ar g Tyr Hi s 310 G u Thr G u Ar g 215 Pr o Al a Al a SIle Pr o 295 Leu Ar g seql i st cor r ect ed. t xt Leu Phe Ala Arg Arg G u 185 Phe Val Pro y Leu Leu 200 205 I e u Lys u Arg y 220 Val Asn Ala Arg Thr G u 235 Su n Ser lie Leu u 250 Arg Tyr Val Leu Ala Pro 265 His IVet Arg Asp Phe Ala 280 285 Val Leu His Ala Val Asp 300 Ile Ala Arg Thr Leu Thr 315 Thr Phe Val Ser Asn Arg 330 Al a 190 Asp SIle Leu G u Ser 270 Al a Asp Phe Pr o Ser Asp Val Val Met 255 Thr Pr o G u Val Thr 335 <210> 585 <211> 1281 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1258) <223> RXC01711 <400> 585 tctcgtgagt ttctccccgg tagcacct t c tat gtgggatagc atcggcaacg cggttgcatg gcc cat Hi s acg Thr t ac Tyr ggc Gy ttc Phe gtt Val gag G u acc Thr gt g Val agt Ser t ac Tyr ct a Leu ctt Leu gct Al a cac Hi s tt g Leu gcg Al a tta Leu ttc Phe gca Al a at t l Ie cga Ar g gt c Val 45 cca Pr o atcagcc cccacgccgc gtcggagcag gttggcc atg ttg ttg atg gcg IVet Leu Leu IVet Ala 1 ggc gca gtc acc gac gat ttc Gy Ala Val Thr Asp Asp Phe 15 ttc gtt gaa ggt att ccg gtc Phe Val u Gy Ile Pro Val ccg cac tac ctc tat aac ccg Pro His Tyr Leu Tyr Asn Pro gga tat atc acc cat ttc acg G y Tyr lie Thr His Phe Thr Page 727 aac Asn cgt Ar g gat Asp ttg Leu ttg gct cgg Leu Al a Ar g tgg atg ttc atc gcg Trp lMt Phe lie Ala 75 eql i st cor r ect ed. t gtg aac ctc ctt Val Asn Leu Leu gcc att gtt tta Ala l e Val Leu gcg Al a gt g Val aac Asn gcg Al a ct a Leu 150 ct c Leu at t SIle gga Gy cga Ar g at g let 230 gt c Val t ac Tyr ttc Phe at g let gcc Al a 310 ggg Gy ccg Pr o ct c Leu 120 ttc Phe at t SIle ct a Leu acc Thr tct Ser 200 ttt Phe acc Thr ct c Leu tgg Tr p t cc Ser 280 ttc Phe gt c Val ct g Leu gcg Al a ttc Phe tgg Tr p ttg Leu ttg Leu 170 gt g Val t ac Tyr aac Asn at g Met gt g Val 250 gca Al a ct g Leu ct g Leu gcc Al a gat Asp 330 acc Thr at c l Ie t cc Ser tgc Cys gcc Al a 155 gt g Val at t SIle gt c Val agc Ser gaa G u 235 ct g Leu acc Thr gga Gy ct c Leu tac Tyr 315 aga Ar g gcc Al a aac Asn gt g Val 140 at t l Ie aaa Lys ttc Phe acc Thr t ca Ser 220 at c SIle gca Al a acc Thr cag G n gga Gy 300 ttc Phe ct c Leu ttg Leu at c SIle 125 gt g Val ttg Leu aag Lys aat Asn cgc Ar g 205 ct c Leu acc Thr ct c Leu acc Thr at g Met 285 agc Ser tta Leu t cc Ser gcg Al a 110 aac Asn cac Hi s at c SIle caa G n gca Al a 190 acg Thr cca Pr o tgg Tr p ct g Leu ggt Gy 270 t ac Tyr cga Ar g ct a Leu ggt Gy 95 at g Met ggc Gy aaa Lys aaa Lys tgg Tr p 175 gt g Val at g Met ggc Gy ttc Phe aga Ar g 255 gt a Val t ac Tyr t cc Ser t cc Ser at g Met 335 tgg Tr p ct g Leu at c SIle aaa Lys ccc Pr o 160 gga Gy gcc Al a ccc Pr o ttg Leu ct c Leu 240 ttc Phe ct c Leu t cc Ser gt a Val cct Pr o 320 gaa G u gcg Al a aca Thr ct g Leu t cc Ser 145 at g Met tcg Ser tgg Tr p t ac Tyr gcc Al a 225 at c SIle cgt Ar g ct g Leu at g Met ttc Phe 305 gac Asp ttt Phe ct g Leu gaa G u ct g Leu 130 tgg Tr p ttc Phe ct c Leu ttc Phe ctt Leu 210 at c l Ie ttc Phe aac Asn act Thr at g Met 290 cac Hi s act Thr ttc Phe agc Ser 100 gt g Val at g let ggc Gy cca Pr o ct c Leu 180 gtt Val gaa G u ttc Phe gca Al a gag G u 260 gt a Val ttc Phe tgg Tr p acc Thr gcg Al a 340 at g Met caa G n ttg Leu gga Gy ctt Leu 165 ggc Gy ccg Pr o act Thr gga Gy at g Met 245 cca Pr o ttc Phe cct Pr o gtt Val t cc Ser 325 acc Thr 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 cag cga cta ccc Sn Arg Leu Pro gta gcc cgc tgg Val Ala Arg Trp Page 728 seqI i st car r ect ed. t xt gt t ggt t gg gga ct a t tg at a gt g gt t aca ttt gt c t cg gcg ct a at c Val G31y Tr p G31y Leu Leu Ilie Val Val Thr Phe Val Ser Al a Leu I Ie 345 350 355 t gg t tt at t ggt gat at c cga gcc aag gga act ccg agc t ca ccc att Tr p Phe Ilie G31y Asp Ilie Ar g Al a Lys G31y Thr Pr o Ser Ser Pro I Ie 360 365 370 acc act gat cca acg cac gac cat ctt gag agg aca gca tgacagactt Thr Thr Asp Pr o Thr Hi s Asp Hi s Leu G31u Ar g Thr Al a 375 380 385 caaact cat c agc <210> 586 <211> 386 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 1171 1219 1268 1281 <400> 586 I'bt Leu Leu I'bt Ala His Arg Phe Phe Val Leu Ala IlieAsn ValI G31u Tyr I Ie Le u Al a Thr Le u Ser 145 vb t Ser Tr p Tyr Al a 225 li1e As p I Ie Tyr Hi s I Ie Ar g Thr 115 Le u Le u Le u I Ie Le u 1 95 G31y Tyr (31y Ph e ValI Pr o Thr Le u (31n Le u Le u 1 65 Pr o Thr 245 Thr Tyr G31y Le u 70 Al a ValI As n Al a Le u 150 Le u I Ie G31y Ar g vb t 230 ValI Thr As n Al a Al a Ph e Tr p Thr I Ie 135 ValI Ph e Le u Al a As p 215 Pr o (31y 10 Tyr Ser Val Ty r Leu Leu Tr p I'b t Leu Leu 90 1I e Al a 1 1 1 e Phe Leu Trp 03 y Leu Pro Leu 1 70 Pr o Val 1 (3 u Ty r Al a As n Tr p I'b t Al a Val 250 Al a Hi s Le u Ph e 75 Thr I Ie Ser Cys Al a 1 55 ValI I Ie ValI Ser G31u 235 Le u Ph e Al a li1e Ar g Al a As n ValI 140 li1e Lys5 Ph e Thr Ser 220 li1e Ar g ValI Pr o Al a Le u Le u I Ie 125 ValI Le u Lys5 As n Ar g 205 Le u Thr 03 y Al a Phe Val Pr o Hi s (3 y Ty r Asn Leu G31y Tr p I'b t Leu G31y I Ie Lys Lys Lys Pr o 1 Tr p G31y 1 Val Al a I'b t Pr o 03 y Leu Phe Leu 240 Ar g Phe 255 Leu Al a Leu Leu Page 729 seql i st cor r ec Arg Asn Thr u Pro Tyr Phe Trp Ala Ala Thr 260 265 Leu Thr G y Val Phe Phe Leu Ser Ser Leu G y 275 280 Met Met I e Phe Pro Mbt IIe Phe Thr Leu Leu 290 295 Phe His Asn Trp Val Ala Trp Val Ala Ala Tyr 305 310 315 Asp Thr Phe Thr Ser G n Arg Leu Pro Asp Val 325 330 Phe Phe Ser Ala Thr Val Gy Trp y Leu Leu 340 345 Val Ser Ala Leu lie Trp Phe Ile y Asp Ile 355 360 Pro Ser Ser Pro lie Thr Thr Asp Pro Thr His 370 375 Thr Al a 385 <210> 587 <211> 777 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(754) <223> RXC02380 <400> 587 gcagattttt gacgaagtcg catcgtggat tgaagctgcc tgctcagcaa ggtggtgtgc gaacactata ctgtccact c ted. txt Thr Thr Sn let 285 G y Ser 300 Phe Leu Al a Ar g Ile Val Arg Ala 365 Asp Hi s 380 Val Tyr Ser Ser Met 335 Thr Gy G u aaa Lys cat Hi s ctt Leu aac Asn gcc Al a cgg Ar g cgc Ar g ggt Gy ct g Leu aaa Lys gcc Al a ggg Gy cgc Ar g ctt Leu gt g Val agc Ser aat Asn act Thr at t SIle gt g Val tac Tyr cca Pr o cca Pr o 75 tac Tyr ggc Gy gcg Al a gtt Val gcg Al a cgg Ar g acg Thr at c SIle ggc Gy ccc Pr o 45 gaa G u ttt Phe at c SIle at g Met t ca Ser acc Thr gat Asp at g Met gat Asp ggt ggc G y G y 15 gag gta G u Val gga cag G y G n cgt tac Arg Tyr gta t cg Val Ser 80 acc ct g Thr Leu Page 73C taaaaactcg cgaggacgca atg act aca acg gtg Met Thr Thr Thr Val 1 aca ttt gac ccc att Thr Phe Asp Pro lie gcg gat cga ttc gat Ala Asp Arg Phe Asp cca tgg caa aag gcg Pro Trp G n Lys Ala ctg atg acg gtg atc Leu bet Thr Val lie cgg gtt gat att gat Arg Val Asp Ile Asp caa gat ttg agc aag G n Asp Leu Ser Lys caa tac ccg gac gcc cag G n Tyr Pro Asp Ala G n 105 gca cag atc gtg acg tgg Ala n Ile Val Thr Trp 120 cac ttc gtt gga gtg act His Phe Val G y Val Thr 135 att ccg gaa atg cac caa Ile Pro u bet His n 150 155 atg gct att tcc tcc acg Met Al a I e Ser Ser Thr 170 cct gtt tgg tat ctt gtc Pro Val Trp Tyr Leu Val 185 cgc caa ctc tat cga cct Arg G n Leu Tyr Arg Pro 200 ggc caa aac caa gca taaa G y n Asn n Ala 215 <210> 588 <211> 218 <212> PRT <213> Corynebacter i um gl <400> 588 Met Thr Thr Thr Val Lys 1 5 Thr Phe Asp Pro lie His Ala Asp Arg Phe Asp Leu Pro Trp G n Lys Ala Asn Leu IVet Thr Val I e Al a 70 Arg Val Asp Ile Asp Arg G n Asp Leu Ser Lys G n 100 G y Al a Asp Al a Leu Al a 115 Thr Phe G u Leu Ala Hi s 130 seql i st cor r ect ed. t xt 95 ctg tac ttc atc acc ggt gcc Leu Tyr Phe Ile Thr y Al a 110 cgc gat tgg gag aaa acc ttc Arg Asp Trp G u Lys Thr Phe 125 130 cga ccc ggt tat gaa ttg gat Arg Pro y Tyr G u Leu Asp 140 145 gat cga gtc tca ttg gtg gat Asp Arg Val Ser Leu Val Asp 160 gac tgc aga gaa cgc tcc agc Asp Cys Arg G u Arg Ser Ser 175 cct gat ggc gtg gtg caa tac Pro Asp y Val Val G n Tyr 190 gaa gga tcc gat aag gat at g G u G y Ser Asp Lys Asp IVet 205 210 .tctagt caaaagtttc aac gat Asp 115 gaa G u gga Sy at c SIle gaa G u at t SIle 195 gat Asp bet Ser Thr Asp Met Asp Phe 110 Tr p Sy 100 gca Al a ctt Leu aac Asn ccc Pr o gaa G u 180 gcc Al a ccc Pr o ut ari cum Arg Arg Ala Asn G y Hi s 25 Asp Leu Val Lys Lys Val 55 Thr Al a Ser G y y Asp Tyr Pro Asp 105 G n Ile Val 120 Phe Val G y 135 Arg lie G y 10 Leu Val Al a Val Tyr Val Ser Pro Al a Asn Pro Ar g Thr Tyr Thr 90 Al a G n Leu Thr Trp Ar g Val Thr Arg 140 Page 731 Sy G u Sy Ar g Val Thr I e G u Tyr seqI i st car r ect ed. t xt Leu Asp Gy Asn Ilie Ilie Pro Ou N/bt His On Asp Arg Val Ser Leu 145 150 155 160 Val Asp I Ie Pr o Ala I'b t Ala I Ie Ser Ser Thr Asp Cys Ar g O31u Ar g 165 170 175 Ser Ser O31u O31u Ar g Pr o Val Tr p Ty r Leu Val Pr o Asp G31y Val Val 180 185 190 O31n Ty r I Ie Ala Lys Ar g O31n Leu Ty r Ar g Pr o O31u O31y Ser As p Lys 195 200 205 As p I'bt As p Pr o Lys G3 y O31n As n O31n A a 210 215 <210O> 589 <21 1> 777 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (754) <223> FRXA02380 <400> 589 gcagatt t tt gacgaagt cg cat cgt ggat t gaagct gcc t aaaaact cg cgaggacgca t gct cagcaa ggt ggt gt gc gaacact at a ct gt ccact c aaa cat Hi s ct t Le u aac As n gcc A a cgg Ar g c aa O31n gca A a c ac Hi s cgc Ar g ggt O31y ct g Le u aaa Lys5 gcc A a ggg O31y ccg Pr o at c I Ie 120 gt t ValI cgc Ar g ct t Le u gt g ValI ag c Ser aat As n act Thr gcc A a ac g Thr gt g ValI at t I Ie gt g ValI t ac Tyr cca Pr o cca Pr o 75 t ac Tyr cag O31n t gg Tr p act Thr ggc O31y gcg A a gt t ValI gcg A a cgg Ar g ac g Thr ct g Le u cgc Ar g cga Ar g 140 at g vb t t ca Ser ac c Thr g at As p at g vb t g at As p tt c Ph e 110 t gg Tr p ggt (31y ggt O31y 15 gag O31u gga O31y cgt Ar g gt a ValI ac c Thr 95 at c I Ie gag O31u ggc O31y gt a ValI c ag O31n t ac Tyr t cg Ser ct g Le u ac c Thr aaa Lys5 at g act I'b t Thr 1 aca ttt Thr Phe gcg gat A a As p cca t gg Pr o Tr p ct g at g Leu I'b t cgg gtt Ar g Val caa gat O31n As p ggt gcc O3 y A a acc tt c Thr Phe 130 g ac As p cga Ar g c aa O31n ac g Thr g at As p tt g Le u g at As p 115 g aa O31u aca acg gt g Thr Thr Val ccc Pr a tt c Ph e aag gt g ValI at t I Ie ag c Ser 1 00 gca A a ct t Le u tat gaa ttg gat gga aac atc Tyr 0 u Leu Asp y Asn Il e 145 Page 732 seqI i st car r ect ed. t xt at t ccg gaa atg cac caa gat cga gt ctca t tggt ggat Ilie Pro O31u Nibt Hi s O31n Asp Ar g Val Ser Leu Val Asp 150 155 160 at ggct at t t cc tcc acg gac tgc aga gaa cgc tcc agc I'b t Ala Ilie Ser Ser Thr Asp Cys Arg O31u Ar g Ser Ser 170 175 cct gt t t gg t at ct t gt c cct gat ggc gt g gt g caa tac Pr o Val Tr p Tyr Leu Val Pr o Asp O31y Val Val O31n Tyr 185 190 cgc caa ct c t at cga cct gaa gga t cc gat aag gat at g Ar g O31n Leu Tyr Ar g Pr o O31u O31y Ser Asp Lys Asp I'b t 200 205 210 ggc caa aac caa gca t aaat ct agt caaaagt t tc aac 03 y 03 n Asn 03 n A a 215 <210> 590 <211> 218 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ari cumn at c ccc gcc Ilie Pr o Ala 1 gaa gaa cgc O31u O31u Ar g 1 att gcc aaa Ile A a Lys 1 gat ccc aag Asp Pro Lys <400> 590 I'bt Thr Thr Thr Val Lys Ar g Ar Thr A a Pr o Le u Ar g O31n O31y Thr Le u 145 ValI Ser O31n As p Ar g O31n Thr As p Le u As p 115 O31u O31y I Ie O31u I Ie 1 95 le As p A a I Ie As p Lys5 Le u A a I Ie A a 1 65 Ar g Lys5 As n As p Lys5 Thr O31y Tyr O31n Ph e 135 Pr o A a ValI O31n A a Hi s ValI ValI Ser As p As p 1 ValI O31y vb t Ser Tyr 1 Tyr I Ie ValI Tyr Pr o Pr a Tyr O31n Tr p Thr O31n 1 Thr ValI Pr o O31y A a ValI A a Ar g Thr Le u Ar g Ar g 140 As p As p Pr a O31u I Ie O31y Pr a O31u Ph e I Ie Tyr As p 125 Pr a Ar g Cys As p O31y 205 O31y O31u O31y Ar g ValI Thr lie O31u Tyr Se r O31u 1 ValI As p Asp I'bt Asp Pro Lys 03 y O31n Asn O31n A a Page 733 seqi i st car r ect ed. t xt 210 215 <210O> 591 <21 1> 594 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (571) <223> RXC02921 <400> 591 t ggaaaact g ggaagggt t g acgt t gcgga at ct ct ccgc aaaagggt ga ggaaccacat gagct gt t tt aaggaat tt t agcgt cggt t cggaccct aa gt g t ct gca ct t gaa 11! Val Ser Al a Leu O31u gag O31u aag Ly s ac c Thr gcc Al a cct Pr o t ac Tyr t ac Tyr gga G31y at c I Ie t ac Tyr 1 50 at c I Ie g at As p t gc cys gt t ValI cgc Ar g tt g Le u ct g Le u g aa O31u 120 aac As n cct Pr o at c lIe at c I Ie gt t ValI gag O31u g aa O31u gt t ValI cgt Ar g tt c Ph e g ac As p gcg Al a gct Al a gt t ValI at c G31y 75 at c I Ie ct g Le u aag Lys ag c Ser ac c Thr gt c ValI gca Al a g aa (31u aac As n c ac Hi s t ac Tyr cgt Ar g at t I Ie 140 gcg gcg Al a Al a 15 gat gt c As p Val gct gac Al a As p gag at g (31u db t gaa aac 31u As n cag cga O31n Ar g 95 gac t gc As p Cys 110 t cc t cc Ser Ser gaa ct C 03 u Leu aaa Ly s t ct Ser aat As n ac c Thr cgt Ar g 80 c ag O31n cca Pr o t gg Tr p cca Pr o 1 gca Al a g ac As p gag O31u aag Ly s 65 t gg Tr p gca Al a ct c Le u t ct Ser cct Pr o gcg Al a at g vb t cgc Ar g 50 gct Al a gt t ValI gag O31u at t I Ie g at As p 130 tt g Le u g at As p at c lIe c ag O31n ggt G31y ct c Le u cgc Ar g g aa O31u 115 gag O31u cca Pr a g aa O31u 20 gca Al a gt g ValI tt c Ph e ct t Le u gag O31u 1 00 at t I Ie gcg Al a gct Al a aag Ly s at c G31y gag O31u g ac As p tt c Ph e g aa O31u g at As p g aa O31u ggc t ac gag gac gat O y Tyr 0 u Asp Asp 155 t aagaggt ag t cct gt gact cgt <210O> 592 <211> 157 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 592 Val Ser Ala Leu Ou Ou Ser lieArg lieAla Thr lie Ala Ala Lys Page 734 seqI i st car r ec 10 Al a Al a Asp G3 u Lys Lys Al aAsp Asp Il e Al a 25 As p N/t I Ie Al a I Ie Thr Asp Cys Phe Val Val 40 31u Ar g 1n Val 31y Al aIlIe Val O31u (1u I Ie 55 Lys Al a O31y Phe O31u Pr o Lys Ar g Ar g O31u O31y 70 Tr p Val Leu Leu As p Ty r O31y Leu Val Val I Ie 90 Ala O31u Ar g O31u Phe Ty r O31y Leu As p Ar g Leu 100 105 Leu I Ie O31u IIe O31u O31y Leu O31u Thr Phe Lys 115 120 Ser As p G3 u Al a As p I Ie Ar g As n I Ie As p Ser 130 135 Pr o Leu Pr o Al a O31u Ty r O31u Pr o G31y Ty r O31u 145 150 155 <210O> 593 <21 1> 3075 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (3052) <223> RXCO1434 <400> 593 ggt t t cct gc gcaccgt gat gat t ggt gcg gcgct gt cgc aacact gcca acacgct gcc caacct gat c act ggaaat c ted. t xt Val I Ie Al a Se r 31u As p Asn Arg Hi s Val Ty r Ar g Ar g O31u 125 1 1 e As p 140 As p As p As p Al a (31u (31u O31n As p 110 Ser a u ValI As p vb t As n Ar g Cys Ser Le u ct g Le u gcc Al a ac g Thr ct g Le u t cc Ser ct g t cg Ser ggc G31y ct g Le u cgg Ar g gcc Al a gcc gt t ValI t cc Ser ggt G31y at g vb t gcg Al a t tc ccg Pr o tt c Ph e ac c Thr t cc Ser t gg Tr p gct ct t Le u ag g Ar g ct g Le u gag G31u ct g Le u tt g acc cgc Thr Ar g 15 cgg ct g Arg Leu t cg at t Ser I Ie gga caa O31y (1n cca cag Pro O31n 80 aac acc Page 73E cggccat cgc t t cggcgt t c gt g t tg ggt gcg gt g 115 Val Leu G31y Al a Val 1 gcg gaa aaa gaa gac 163 Al a 03 u Lys 03 u Asp ct c acc ct g t cg gt g 211 Leu Thr Leu Ser Val atc ggc gcg ccg ctg 259 Ilie G31y Al a Pr o Leu gt c aac gt g gt c at g 307 Val Asn Val Val I'b t at t t tc t tc t ac ggc 355 Il e Phe Phe Tyr 03 y cgt gaa gt g t tc aaa 403 Leu Phe Ala Leu Phe Mbt Ala seql i st cor r ect ed. t xt Val Leu Asn Thr Arg G u Val Phe Lys 100 ccc Pr o ct g Leu gt g Val ctt Leu 150 gcg Al a caa G n cag G n gct Al a t at Tyr 230 t cc Ser cag G n ttc Phe ggc Gy ttc Phe 310 cgt Ar g gcc Al a gcg Al a gt g Val 120 at t SIle gt g Val at t l Ie ggt Gy ggt Sy 200 ccg Pr o at c SIle aac Asn ggt Sy acc Thr 280 ttc Phe gct Al a ttt Phe at c l Ie gca Al a at g Met gat Asp gca Al a at g Met 170 at g let at c SIle at t SIle ggc G y gca Al a 250 aag Lys ttc Phe gcc Al a acg Thr gcg Al a 330 gcc Al a cct Pr o gt g Val ccg Pr o cag G n 155 cgc Ar g gcg Al a at c SIle t at Ty r gt c Val 235 gac Asp ct a Leu ggt G y aac Asn ct g Leu 315 cgt Ar g acc Thr gtt Val ct g Leu cag G n 140 tgt Cys cct Pr o at g let acc Thr cag G n 220 acc Thr ggc G y acc Thr gt c Val gcc Al a 300 at t SIle gaa G u aag Lys gt c Val cct Pr o 125 at c SIle ct a Leu ct g Leu gcg Al a act Thr 205 cag G n ttg Leu gat Asp ttc Phe cct Pr o 285 gcc Al a cct Pr o gag G u gt c Val aac Asn 110 gcg Al a at t SIle at c SIle tgg Tr p at c l Ie 190 cgc Ar g cac Hi s ct c Leu gat Asp at c SIle 270 at t SIle aac Asn t ac Tyr gt c Val gt g Val 350 aat Asn cgt Ar g ttc Phe at g Met ggt G y 175 at c SIle at t SIle tgg Tr p acc Thr agg Ar g 255 gca Al a gcc Al a at c SIle gct Al a tgg Tr p 335 ctt Leu gt g Val ttg Leu ct c Leu at t SI e 160 at c SIle gt g Val gcg Al a at g Met gcg Al a 240 gca Al a ct g Leu aat Asn ctt Leu ttg Leu 320 acc Thr t cc Ser acc Thr ccg Pr o 130 gt g Val t ac Tyr gcg Al a gt g Val at t l Ie 210 ct g Leu at g let gt c Val ccc Pr o ctt Leu 290 tgg Tr p ct g Leu acc Thr ttg Leu ttg Leu 115 cat Hi s ggc G y ct g Leu cgt Ar g gca Al a 195 gca Al a caa G n ccg Pr o tct Ser at c SIle 275 ttt Phe act Thr ct a Leu ttc Phe gca Al a 355 acc Thr gag G u acc Thr cgt Ar g ttg Leu 180 at c SIe gac Asp gtt Val cga Ar g gac Asp 260 gt g Val gcc Al a ct g Leu cat Hi s at c SIe 340 ccg Pr o 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 ctg tcg agc tcc ccg gag cgt gtg gtg gtg ctt ctt ggt gcg gcc aac Page 736 Leu Ser Ser Ser Pro G3 u Ar g 360 seqi i st car r ect ed. t xt Val Val Val Leu Leu G3 y 365 370 A a A a As n ggt G31y aac As n 390 t gg Tr p ggg G31y ag c Ser at c I Ie c aa O31n 470 at t I Ie c ag O31n cct Pr o gt t ValI c ac Hi s 550 ac c Thr cca Pr o gt g ValI gcc A a agt Ser ct c Le u tt g Le u ct g Le u gt a ValI 440 gt c ValI tt g Le u ccg Pr o tt c Ph e ccg Pr o 520 ggc G31y ggc G31y aag Ly s gcg A a cgc Ar g 600 aac As n at c I Ie ct g Le u t ct Ser 410 c aa O31n t ac Tyr G31y c ag O31n ac c Thr 490 ac c Thr ccg Pr a cca Pr a c aa O31n gt c ValI 570 ct g Le u ac c Thr c ac Hi s acc Thr t t g Le u 395 gcg A a gcc A a t t g Le u at c I Ie gca A a 475 aag Ly s cag O31n at g vb t gt c ValI ggc O31y 555 gcg A a t ct Ser aag Ly s t cc Ser ggc O31y 380 ggt O31y gcg A a gt c ValI tt g Le u gt g ValI 460 ct g Le u at c I Ie ct g Le u t cc Ser ggc O31y 540 gcg A a ct g Le u t cg Ser aag Ly s gag O31u 620 gcg A a at g vb t gt t ValI gt g ValI aac As n 445 tt g Le u ac c Thr t ct Ser gt c ValI gcc A a 525 g ac As p cgt Ar g at c I Ie gca A a ct g Le u 605 gcg A a gt c ValI cgc Ar g ggt O31y ggc O31y 430 ct g Le u t ca Ser cgc Ar g tt g Le u gcg A a 510 ggt O31y ggt O31y Ph e tt t Ph e gcc A a 590 gcc A a t ac Tyr at c I Ie t ct Ser gca A a 415 g at As p gct A a cgt Ar g at c I Ie g at As p 495 cca Pr a at t I Ie cgt Ar g t gg Tr p gt g ValI 575 gca A a ag c Ser cgc Ar g ggc O31y tt g Le u 400 gca A a tt c Ph e gt g ValI t ct Ser cca Pr a 480 gt c ValI ag c Ser gt c ValI tt c Ph e c ag O31n 560 g at As p gcg A a tt g Le u aac As n gcg A a 385 gct A a gca A a tt g Le u tt g Le u ggt O31y 465 ggt O31y ggc O31y gag O31u cgc Ar g cgt Ar g 545 gcc A a act Thr ggc O31y ggc O31y ggt O31y 625 t at Tyr aaa Ly s gca A a ct g Le u ggt O31y 450 tt g Le u tt g Le u g aa O31u tt c Ph e gga O31y 530 ct g Le u cgc Ar g t cc Ser at c I Ie ag c Ser 610 t gc Cys ct g Le u ac c Thr t gg Tr p ggc O31y 435 gt c ValI ccg Pr a ag c Ser gt c ValI gca A a 515 cct Pr a ct c Le u gag O31u O31y gcc A a 595 tt g Le u ct c Le u tt g Le u t cc Ser gcg A a 420 act Thr tt c Ph e gag O31u cgg Ar g t cc Ser 500 gca A a cgc Ar g gcc A a at c I Ie aac As n 580 t ac Tyr gcg A a at t I Ie cgc Ar g ct g Le u 405 tt g Le u ct a Le u tt c Ph e gt c ValI tt t Ph e 485 gag O31u ac c Thr ct g Le u g at As p gcc A a 565 gcc A a gag O31u gt a ValI gt g ValI 1267 1315 1363 1411 1459 1 507 1 555 1 603 1651 1 699 1 747 1 795 1 843 1891 1 939 1 987 2035 gcc gat t gg gt gcct ggc tcc agct t gagc gcc gt cgcg gaa tcc ggt Page 737 seqi i st car r ect ed. t xt r Leu Ser A a Val A Ala Asp Trp Val Pro 630 G3 y Se r Se 635 a G3 u Se r G3 y 645 gcc A a at c I Ie t gc cys gcg A a t cg Ser 710 aag Ly s g at As p gag (31u gcg A a gca A a 790 ggt G31y t cc Ser gt g ValI g ac As p ac c Thr 870 ac g Thr ccc Pr o gag (31u at t I Ie 680 tt g Le u gcc A a at g vb t gca A a cca Pr o 760 cgt Ar g at c I Ie gcg A a tt g Le u cca Pr o 840 gca A a t gg Tr p gcc A a gcc A a 650 c ac Hi s at c I Ie g at As p at g vb t act Thr 730 ct t Le u c ac Hi s ggt G31y gct A a ct c Le u 810 t ct Ser gt c ValI ac a Thr t cc Ser ct c Le u 890 gcc A a gag (31u aac As n gcc A a ct t Le u 71 5 gaa (31u gcc A a ct t Le u t t c Ph e gt g ValI 795 acc Thr gt t ValI t ac Tyr g at As p acc Thr 875 gac As p gcg A a at g vb t ac c Thr t ca Ser 700 at c I Ie gcc A a at g vb t gt g ValI ggt G31y 780 gt g ValI ag c Ser g aa (31u ct g Le u gt c ValI 860 ggc G31y cgc Ar g tt c Ph e ggt G31y g ac As p 685 gag (31u g ac As p c ag O31n gcg A a gt g ValI 765 gcc A a at c I Ie tt c Ph e gcc A a g at As p 845 ac c Thr ggc G31y gt c ValI gcg A a at c I Ie 670 ggc G31y ct c Le u gcg A a ggg G31y at g vb t 750 aag Ly s t cc Ser at c I Ie tt c Ph e ac c Thr 830 ct g Le u g ac As p g ac As p at c I Ie 640 gcg A a gcc A a gcc A a g ac As p ct c Le u 720 gct A a ac c Thr g aa G31u t ac Tyr gt t ValI 800 ag c Ser t ct Ser c aa O31n ac c Thr ct c Le u 880 ac c Thr g aa O31u G31y gt c ValI gcc A a 705 gct A a ac a Thr t gc cys aag Ly s ac c Thr 785 t cc Ser ag c Ser gca A a gcc A a g ac As p 865 ct a Le u ac c Thr ct a Le u tt g Le u ct c Le u 690 aag Ly s ccc Pr o g aa O31u gga G31y ac a Thr 770 gt c ValI ct g Le u ac c Thr ac a Thr cgc Ar g 850 ggc G31y gt t ValI G31y act Thr g ac As p 675 gcc A a t cc Ser ag c Ser g ac As p ct g Le u 755 cca Pr a aag Ly s gt g ValI aac As n cca Pr a 835 ac g Thr aac As n g ac As p ac c Thr g aa O31u 660 aac As n tt g Le u ct g Le u g ac As p aat As n 740 tt c Ph e aag Ly s ggc G31y gcc A a g aa O31u 820 g aa O31u t gg Tr p ac c Thr ct g Le u ggc G31y 900 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 gac agc aac gtg acc tcg acc gtg aag atc tac gca ttc aac gac gcc Page 738 As p t ca Ser t cc Ser ggt G31y 950 c aa O31n gt t ValI As n c ac Hi s 920 cgc Ar g gt g ValI t ca Ser t gg Tr p seqI i st car r ect ed. t xt Val Thr Ser Thr Val Lys iie Tyr Al a Phe 905 910 tcc ctg tcg gaa ggc atc gag atc ggc acc Ser Leu Ser O31u 31y Ilie O31u Ilie 31y Thr 925 930 agt ct c agc cac agc at c cgc gat t cc t cc Ser Leu Ser Hi s Ser Ilie Ar g Asp Ser Ser 940 945 gaa t cc gt g gt g at t ct g gt c gat gag gt t O31u Ser Val Val Ilie Leu Val Asp O31u Val 955 960 gac acc aat cca cag at g cag at c gct gaa Asp Thr Asn Pro O31n b t O31n Ilie Ala O31u 970 975 t aaat tacgc gt t tgt gat t gac As n 915 gt g ValI aag Ly s cgt Ar g gt a ValI As p g at As p ct t Le u t cc Ser c aa O31n 980 2899 2947 2995 3043 3075 <210O> 594 <21 1> 984 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 594 ValI Al a Le u I Ie ValI li1e Ar g I Ie Hi s O31y 145 Pr a As p Tyr Leu 03 y Al a Val O3 u Lys 03 u Thr Leu Ser O31y Al a Pro As n Val Val Phe Phe Tyr O31u Val Phe 1 00 Thr Leu Thr 115 Pr o Hi s O31u 130 Val O31y Thr Tyr Leu Arg Al a Ar g Leu 1 80 Val Al a Ilie 1 95 As p ValI Le u vb t O31y Ly s ValI O31n Thr Ar g 1 65 Ly s Ser Leu Thr Ser Leu Val 10 Al a As p 03 y 03 y Se r Thr Leu Leu O31y O31y 40 Leu Thr Ar g 'b t I'b t Ser Thr Al a Phe Al a 70 Leu Phe Al a Leu Phe 90 Pro O31y Al a Tr p Al a 1 Leu 03 y Val Ty r Nib t 120 Val O31y I Ie Phe As p 135 Leu O31y Val Val Al a 150 Al a 03 y I I e As p I'b t 1 70 O3 n Phe 03 y 03 y I'b t 1 (31n Phe (31y Ty r I Ie 200 I Ie O31y ValI Le u Tyr vb t Pr a ValI Pr a O31n 1 Ar g Al a ValI Ph e I Ie Ser Le u ValI ValI Pr a 125 li1e Le u Le u Al a Thr Ar g Ser O31y Pr a As n As n Ar g Ph e vb t O31y 1 li1e I Ie Th r Th r Ar g I Ie Al a 205 Page 739 seqI i st car r ect ed. t xt Ser Ilie Ala Asp Asp Ala Ala Pro Phe Ilie Tyr O31n O31n Hi s Tr p I'bt 210 Le u 225 li1e ValI I Ie O31y O31y 305 ValI Pr o Le u Le u A a 385 A a A a Le u Le u O31y 465 O31y O31y O31u Ar g Ar g 545 O31n Pr o Ser I Ie 275 Ph e Thr Le u Ph e A a 355 A a Le u Thr Tr p G31y 435 ValI Pr o Ser ValI A a 515 Pr o Le u Pr a Le u 245 Le u ValI Tyr Ser Le u 325 li1e Le u As n Ar g Le u 405 Le u Le u Ph e ValI Ph e 485 (31u Thr Le u As p Tyr 230 Ser O31n Ph e O31y Ph e 310 Ar g A a Le u O31y As n 390 Tr p O31y Ser I Ie O31n 470 li1e O31n Pr a ValI Hi s 550 215 O31y Ar g Le u Ph e O31n 295 Ser ValI G y Ser Ph e 375 Lys5 A a Tr p Ser Ph e 455 As n Ar g As p ValI Pr a 535 O31y I Ie As n O31y Thr 280 Ph e A a Ph e I Ie Ser 360 Ser Le u Le u Le u ValI 440 ValI Le u Pr a Ph e Pr a 520 O31y O31y I Ie A a Ser 265 A a As p Ph e Tyr Thr 345 Ser Ph e O31y O31y I Ie 425 O31y Thr O31y As n Ser 505 Pr a A a ValI O31y A a 250 Lys5 Ph e A a Thr A a 330 A a Pr a I Ie Le u Ser 410 O31n Tyr O31y O31n Thr 490 Thr Pr a Pr a O31n ValI 235 As p Le u O31y As n Le u 315 Ar g Thr O31u Thr Le u 395 A a A a Le u I Ie A a 475 Lys5 O31n vb t ValI O31y 555 220 Thr O31y Thr ValI A a 300 li1e O31u Ly s Ar g O31y 380 O31y A a ValI Le u ValI 460 Le u I Ie Le u Ser O31y 540 A a Le u As p Ph e Pr a 285 A a Pr a O31u ValI ValI 365 A a vb t ValI ValI As n 445 Le u Thr Ser ValI A a 525 As p Ar g Thr Ar g 255 A a A a I Ie A a Tr p 335 Le u ValI I Ie Ser A a 415 As p A a Ar g I Ie As p 495 Pr a I Ie Ar g Tr p Ala Arg Ou Ilie Ala Thr Oy Lys Ou Val Ala Leu I Ie Phe Val Asp Page 740 seql i st cor r ect ed. t xt 565 Ala Pro Phe Ala Thr Gy Gy Gy 625 Val G u Gy Val Al a 705 Al a Thr Cys Lys Thr 785 Ser Ser Al a Al a Asp 865 Leu Thr Al a Gy Al a 595 Leu Leu G u Thr Asp 675 Al a Ser Ser Asp Leu 755 Pr o Lys Val Asn Pr o 835 Thr Asn Asp Thr Asn 915 u Val Val Gy 645 Thr Lys Pr o Al a Val 725 Pr o Thr Pr o vbt Al a 805 G n Pr o Asp Ser Ser 885 Ser Al a Val Al a Al a 630 Al a SIle Cys Al a Ser 710 Lys Asp G u Al a Al a 790 Gy Ser Val Asp Thr 870 Thr Asp Ser n Pr o 615 Asp Asp Gy Ar g l Ie 695 Al a Al a Tyr G u Thr 775 Al a Thr Pr o Gy Gy 855 Al a Pr o Ser Pr o Ar g 600 Asn Tr p Pr o G u SIle 680 Leu Al a b t Al a Pr o 760 Ar g l Ie Al a Leu Pr o 840 Al a Tr p Al a Asn Hi s 920 570 Pro Leu Ser 585 Arg Thr Lys Ile His Ser Val Pro G y 635 Ar g Al a Al a 650 Ala His u 665 Arg lie Asn Pro Asp Al a G u Iet Leu 715 Val Thr G u 730 Ser Leu Al a 745 Thr Hi s Leu Asp G y Phe Al a Al a Val 795 Phe Leu Thr 810 Al a Ser Val 825 Pro Val Tyr G y Thr Asp Thr Ser Thr 875 Arg Leu Asp 890 Val Thr Ser 905 Ser Leu Ser Ser Al a Al a 590 Lys Leu Al a 605 G u Al a Tyr 620 Ser Ser Leu Al a Phe Al a 'e t Gy lie 670 Thr Asp G y 685 Ser G u Leu 700 I e Asp Al a Al a G n y Mvt Al a MVet 750 Val Val Lys 765 G y Al a Ser 780 Val Ile lle Ser Phe Phe G u Al a Thr 830 Leu Asp Leu 845 Val Thr Asp 860 G y y Asp Arg Val lle Thr Val Lys 910 575 Al a Ser Ar g Ser Leu 655 Pr o Hi s Ar g Thr Leu 735 Ar g Lys Asp Leu Gy 815 Thr Asp Val Gy Leu 895 I le Al a Leu Asn Al a 640 Al a Al a Al a Asp Leu 720 Al a Thr G u Tyr Val 800 Ser Ser G n Thr Leu 880 Thr Tyr G u Gy 925 Il e u lie Page 741 G3 y Th r 930 Ser Ser 945 G3 u Val Al a G3 u seqI i st car r ect ed. t xt Val Asp Tyr Ser O31y Ar g Ser Leu Ser His Ser IlieArg Asp 935 940 Lys Leu Pr o O31y O31n Val O31u Se r Val Val I Ie Leu Val As p 950 955 960 Ar g Ser Ser O31n Thr Ser Asp Thr Asn Pro O31n I'bt O31n Ilie 965 970 975 Val O31n Leu Val O31y Tr p 980 <210O> 595 <21 1> 915 <21 2> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (892) <223> RXA01807 <400> 595 gct caccgag ct ggacacca agct ccgcgc agt gcaggaa gcagt ggct g gaact cggcg aggaaat cga gggct agt t c gaacacggcg agct ggaaat atg ccg tcg gca ggc 11! I'bt Pro Ser Al a O31v gag O31u gcc Al a ct c Le u gct Al a c ac Hi s ct g Le u t at Tyr aaa at g vb t at t I Ie gt g ValI gct Al a ccc Pr a c ag O31n g at As p ac c Thr cgc Ar g 120 g ac As p gag O31u at g vb t at t I Ie gt g ValI at g vb t ggg O31y gct Al a ac c Thr g ac As p cgc Ar g ggc O31y gt g ValI tt a Le u g ac As p at c I Ie c ag O31n ccg Pr a gga O31y 140 gca Al a ag c Ser at g vb t 45 agt Ser c aa O31n g at As p gt g ValI cat Hi s 125 at t I Ie c ag O31n c ag O31n c ac Hi s t cc Ser t gg Tr p gag O31u cgt Ar g 110 gt g ValI t at Tyr gag O31u gt t ValI g aa O31u ccg Pr a gcc Al a t cc Ser gt c ValI at c I Ie gcc Al a g at As p ggt O31y ct c Le u t at Tyr gcg Al a at t I Ie gcg Al a gt g ValI 130 gag O31u cgc Ar g gt g ValI gt g ValI agt Ser c aa O31n ac c Thr 1 00 ct g Le u ccc Pr a gca Al a gcc at c t gc cag gac Al a I e Cys 0 nAsp t ta t gc cct ct g gct acc gga Leu Cys Pro Leu Al a Thr O31y Page 742 at c at t ccc aat Ilie Ilie Pro Asn seql i st cor r ect ed. t xt 150 155 160 165 gct ttc gag ctc tcc cac atg gtt ggc tcc ggc gat ccg cgc tcg ctg 643 Al a Phe G u Leu Ser Hi s IVbt Val G y Ser G y Asp Pro Arg Ser Leu 170 175 180 ctc ggc ccg ttt ggc gag tgg atc atc atc acc agc gcc act gaa act 691 Leu y Pro Phe Gy u Trp Ile Ile I e Thr Ser Ala Thr u Thr 185 190 195 gtg ggc acc acc gtc acc cgc atc gtc acc cgt gac agc gtc cag gaa 739 Val y Thr Thr Val Thr Arg lie Val Thr Arg Asp Ser Val n G u 200 205 210 atc gcc tcc gcc acc gtc gat acc acg gcc aaa ggg gca ggc gac gtc 787 Ile Ala Ser Ala Thr Val Asp Thr Thr Ala Lys y Ala y Asp Val 215 220 225 tac gcc gca gca tta atc gcc gcc ctg cat aaa gat ttt tcg ctt atc 835 Tyr Ala Ala Ala Leu Ile Ala Ala Leu His Lys Asp Phe Ser Leu Ile 230 235 240 245 gac gcc gcc agc cac gca tcc aac acc gtc tgc gcc ggc ctg cag acc 883 Asp Ala Ala Ser His Al a Ser Asn Thr Val Cys Ala G y Leu G n Thr 250 255 260 aaa gcg ctt taggtttcgt ccgtctctga cag 915 Lys Ala Leu <210> 596 <211> 264 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 596 IVbt Pro Ser Ala Gy Ou Ou Ile Leu Ou n Arg Ala n Leu u 1 5 10 Phe Asp n Arg Arg Al a Asp Val Val IVbt I Ie G y Ser G n Val Val 25 Tyr G y Ser Val G y Leu Ser Ala Ala lie Pro Val IVbt His Asn G u 40 G y Leu Arg Val Val Ala Val Pro Thr Val Val Leu Ser Ser lVbt Pro 55 Arg Tyr Ala Ser Ser His Arg G n Pro IVbt Ser Asp G n Trp Leu Ala 70 75 Asp Ala Leu G n Asp Leu Val Asp Leu y lie lie Asp G u Val Ser 90 Thr lie Ser Thr y Tyr Phe Thr Ser Ala Ser G n Val Arg Val Val 100 105 110 Ala Ala Trp Leu G n Lys Ile Arg G u Thr His Pro His Val Arg Ile 115 120 125 Val Val Asp Pro Ile IVbt G y Asp Ser Asp Val G y Ile Tyr Val Al a 130 135 140 Asp G u Ile Ala Thr Al a lle Cys G n Asp Leu Cys Pro Leu Ala Thr 145 150 155 160 G y lie lie Pro Asn Ala Phe G u Leu Ser His IVbt Val G y Ser G y Page 743 Asp Ser Asp Gy 225 Asp Al a Ar g Thr 195 Val Gy Ser Leu 165 Leu Thr G u Val SIle 245 Thr Gy Gy Al a 215 Al a Al a Al a seql i st cor r ect ed. t xt 170 Pro Phe Gy Ou Trp lie 185 Thr Thr Val Thr Arg Il e 200 205 Ser Ala Thr Val Asp Thr 220 Ala Ala Leu i e Ala Al a 235 Ala Ser His Ala Ser Asn 250 Leu 175 I e Thr Al a Hi s Val 255 <210> 597 <211> 1461 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1438) <223> RXN02754 <400> 597 attatgaagc catcggagtt ggtgtggcct acaagggtga agttcactgt agctcccgct gattccgtag aatcaacaga tcatgcgtgg atagtggtgg gt g Val aat Asn tct Ser gag G u ccc Pr o t ac Tyr ttt Phe cgt Ar g ggc Gy gt g Val ttc Phe acc Thr acg Thr gt c Val ttc Phe ct g Leu cgc Ar g ggc Gy t ca Ser ctt Leu gag G u gca Al a gag G u t ac Tyr ggc Gy ttt Phe aac Asn caa G n gt c Val gga Gy gaa G u 75 ct c Leu gaa G u gca Al a cgt Ar g gca Al a ttt Phe aca Thr caa G n cgc Ar g at c SIle gaa G u t ca Ser gcg Al a agc Ser 45 gca Al a ct c Leu aac Asn t ac Tyr tgc Cys 1 aca gct ctc ctt act Thr Ala Leu Leu Thr 15 ctc gct gat ggt t ct Leu Al a Asp G y Ser cgc cgc ctc ccc aac Arg Arg Leu Pro Asn cga gtg ctg aag gcg Arg Val Leu Lys Al a gcc gat ctt gac ttt Ala Asp Leu Asp Phe 80 tac cga ttc acc ggc Tyr Arg Phe Thr y 95 ttc ccg cag tcc cct Phe Pro G n Ser Pro 110 gtc atc cta gaa act Val lie Leu G u Thr Page 744 acc aat ccg Thr Asn Pro gat aaa t at Asp Lys Tyr gca gaa cgc Al a G u Ar g gag cgc cga G u Arg Arg att cgt gac lie Arg Asp tta gac gac Leu Asp Asp caa gt t gat G n Val Asp 100 ctt ctg act Leu Leu Thr 115 gtc att ttg Val Ile Leu seql i st cor r ect ed. t xt t cc at c Ser Ile 135 atg aat gca gat IVet Asn Ala Asp gcc gtc gct tcc Ala Val Ala Ser 130 gct gcg cgc atg Ala Ala Arg IVet gcc Al a 145 gtc Val 150 acc Thr acc gca gct gat Thr Ala Ala Asp cac gaa tac tcg His G u Tyr Ser 170 cgc ccc atc atc Arg Pro Ile Ile gaa G u 160 atg gga tcc agg Met y Ser Arg gca gtc acc gca Ala Val Thr Ala cgc gca gca tac Arg Ala Ala Tyr ctc gct Leu Al a 180 gga ttc tcc G y Phe Ser cca gca t cc Pro Al a Ser 200 acc tcc aac ctc Thr Ser Asn Leu gag G u 190 gcg gcc tac cgc Ala Ala Tyr Arg t ac gga at t Tyr G y Ile 195 cac atc aac Hi s I e Asn gga acc tcc gcc G y Thr Ser Al a gca tgg act ttg Ala Trp Thr Leu ctg Leu 210 gat gac Asp Asp 215 ggc acc ccc aac Sy Thr Pro Asn gaa G u 220 gca gca gct ttc Al a Al a Al a Phe aaa Lys 225 gca cag gtt gaa Ala n Val u t cc Ser 230 ctc ggc gtg gac Leu y Val Asp acc ttg ctg gta Thr Leu Leu Val gat Asp 240 act tat gac atc Thr Tyr Asp Ile caa ggt gtg gcc Sn y Val Al a acc Thr 250 gcc att gaa gtt Al a lle G u Val gca Al a 255 ggt cca gac ctt G y Pro Asp Leu ggt ggc y260 260 gt a cgt at c Val Arg lle aag cag ct c Lys G n Leu 280 tcc ggc gac cta Ser G y Asp Leu ggt G0y 270 gtg ctt gcc cga Val Leu Ala Ar g aag gtc cgc Lys Val Arg 275 gtg gtc tcc Val Val Ser gac gat ctc aac Asp Asp Leu Asn gcc Al a 285 cac aac acc aag His Asn Thr Lys at t Ile 290 t cc gac Ser Asp 295 ctg gat gaa ttc Leu Asp G u Phe gcc Al a 300 atc gcg ggt ctt lle Al a y Leu ggc gaa cca gtt G y G u Pro Val gac Asp 310 gtc ttt ggc gtt Val Phe G y Val acc tcc gtt gtc Thr Ser Val Val aca ggt tct ggc gca cca Thr y Ser y Ala Pro 320 325 acc gct ggc ct c Thr Ala G y Leu gt g Val 330 tac aag atc ggg Tyr Lys Ile G y gaa G u 335 gtt gcc ggt cac Val Ala G y Hi s cct gtg Pro Val 340 gcc aag cgt Ala Lys Arg gtg cgc acc Val Arg Thr 360 cga aac aag gaa Arg Asn Lys G u agc tac ggt ggt ggc aag aag gct Ser Tyr G y G y G y Lys Lys Al a 350 355 1027 1075 1123 1171 1219 1267 1315 cac cgc aag tcc His Arg Lys Ser acc gca atc gaa Thr Ala lIe G u gaa G u 370 atc gtc tac Ile Val Tyr cca ttc Pro Phe 375 aat gcc gaa gca Asn Ala G u Al a cca gat act gga aag ctc gac act ttg agc Pro Asp Thr y Lys Leu Asp Thr Leu Ser 380 385 ctg acc atc cca ttg atg cgc gac ggt Leu Thr lie Pro Leu IVet Arg Asp y gaa atc gtt cca ggt ttg cct Su Ile Val Pro y Leu Pro Page 745 seqi i st car r ect ed. t xt 390 395 400 405 act ttg gaa gat t cc cga gcg tat t t ggcc aag caat t ggt c tct tt a Thr Leu O31u Asp Ser Ar g Ala Ty r Leu Ala Lys O31n Leu Val Ser Leu 410 415 420 cca t gg gaa ggc ct t gca ct g t ct cgc gat gag cct gt t t tg cac act Pr o Tr p O31u 31y Leu Ala Leu Ser Ar g Asp O31u Pr o Val Leu Hi s Thr 425 430 435 cgt t tc gt g ggt t tc ccg ccg gcc gct t agacaat tc ggt ct cacca Ar g Phe Val G31y Phe Pr o Pr o Ala Ala 440 445 aac <210> 598 <211> 446 <21 2> PRT <21 3> Cor ynebact er i urn gl ut ani cumn 1363 1411 1458 1461 <400> 598 Val Asn Thr Asn Pro Ser O u Phe Ser Le u G31y Pr o As p Thr Ser O31u A a 145 vb t A a Tyr Le u 225 As p A a O31u I Ie Le u O31n Le u 115 ValI A a Ser Tyr Tyr 1 95 Hi s O31n Tyr Ar g Ar g As p As p As p Thr Le u vb t Ar g 1 65 A a I Ie As n (31u Le u Ser G31y ValI Thr Tyr Ar g I Ie 135 Thr Hi s Ph e A a As p 215 Le u vb t Ph e ValI Thr (31u (31u 1 05 Thr As n A a Tyr Thr 1 85 G31y Thr ValI Ser 10 Le u (31u A a (31u Tyr 90 G31y Ph e A a As p Ser 1 70 Thr Thr Pr o As n O31n ValI O31y O31u Le u O31u A a As p O31y 1 A a Ser Ser As n Ar g A a Ph e Thr O31n Ar g I Ie O31u Ser 140 Ar g ValI As n A a O31u 220 Ser A a Ser A a Le u As n Tyr cys 125 A a Pr o Thr Le u Hi s 205 A a Thr Le u Ar g Ar g A a Tyr Ph e 110 ValI ValI I Ie A a O31u 1 A a A a A a A a Ar g ValI As p Ar g Pr a I Ie A a I Ie Ser 1 A a Tr p A a Asp Thr Thr Leu Leu Val 235 Page 746 seql i st cor r ect ed. t xt Val Ala Thr Ala lle u Thr Tyr Asp Ile Thr G n y 245 Val Ala y 255 Pr o Al a Lys Ar g 305 Sy Al a Sy G u Leu 385 Val G n Pr o Leu Lys 275 Val G u Gy Hi s Lys 355 I le Thr Gy Val Leu 435 Gy Ar g Ser Val Pr o 325 Val Al a Tyr Ser Pr o 405 Leu Thr Ar g G n Asp 295 Val Al a Lys Ar g Phe 375 Thr Leu Tr p Phe Asp 265 Asp Asp Gy Leu Ser 345 Hi s Al a Pr o Asp Gy 425 Gy Gy Leu Phe Gy 315 Tyr Asn Lys Al a Met 395 Ar g Al a Pr o Asp Asn Al a 300 Thr Lys Lys Ser Pr o 380 Ar g Al a Leu Pr o Leu Al a 285 l le Ser SIle G u Gy 365 Asp Asp Tyr Ser Al a 445 Gy 270 Hi s Al a Val Gy Ser 350 Thr Thr Gy Leu Ar g 430 Al a Val Asn Gy Val G u 335 Tyr Al a Gy G u Al a 415 Asp <210> 599 <211> 871 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(871) <223> FRXA02405 <400> 599 attatgaagc catcggagtt ggtgtggcct acaagggtga agttcactgt agctcccgct gattccgtag aatcaacaga tcatgcgtgg atagtggtgg gtg aat acc aat ccg tct gaa ttc Ser G u Phe gag ct g acc G u Leu Thr ccc tca acg Pro Ser Thr tcc tca aac cgt tca aca Ser Ser Asn Arg Ser Thr atg ctt caa gca gcg ctc IVet Leu G n Ala Ala Leu 30 ttt gag gtc ttt agc cgc Phe u Val Phe Ser Ar g 45 Val Asn Thr Asn Pro 1 gct ctc ctt act gat aaa tat Ala Leu Leu Thr Asp Lys Tyr 15 gct gat ggt t ct gca gaa cgc Ala Asp y Ser Ala G u Arg cgc ctc ccc aac gag cgc cga Arg Leu Pro Asn G u Arg Arg Page 747 seql i st cor r ect ed. t xt t ac Tyr ttt Phe cgt Ar g ggc G y gt g Val t cc Ser gt c Val 150 acc Thr gga Gy cca Pr o gat Asp t cc Ser 230 caa G n gt c Val ttc Phe ct g Leu cgc Ar g ggc G y 120 at g Met gca Al a gaa G u t cc Ser t cc Ser 200 ggc Gy ggc G y gt g Val gca Al a gag G u t ac Tyr ggc G y ttt Phe gca Al a gat Asp tcg Ser 170 acc Thr acc Thr ccc Pr o gac Asp gga Gy gaa G u 75 ct c Leu gaa G u gca Al a gat Asp ggt Gy 155 gca Al a t cc Ser t cc Ser aac Asn acc Thr 235 aca gca cga Thr Al a Ar g caa ct c gcc G n Leu Al a cgc aac tac Arg Asn Tyr atc tac ttc Ile Tyr Phe 110 gaa t gc gt c G u Cys Val 125 tct gcc gt c Ser Al a Val 140 cgc ccc at c Arg Pro Ile gtc acc gca Val Thr Al a aac ctc gag Asn Leu G u 190 gcc cac gca Al a Hi s Al a 205 gaa gca gca G u Ala Al a 220 acc ttg ctg Thr Leu Leu gt g Val gat Asp cga Ar g 95 ccg Pr o at c SIle gct Al a at c SIle t cc Ser 175 gcg Al a tgg Tr p gct Al a gt a Val ctg aag gcg Leu Lys Al a ctt gac ttt Leu Asp Phe 80 ttc acc ggc Phe Thr G y cag t cc cct G n Ser Pro ct a gaa act Leu G u Thr 130 t cc gcc gct Ser Al a Al a 145 gaa atg gga G u vet y 160 cgc gca gca Ar g Al a Al a gcc tac cgc Al a Tyr Ar g act ttg ctg Thr Leu Leu 210 ttc aaa gca Phe Lys Al a 225 gat act tat Asp Thr Tyr 240 at t l Ie tta Leu caa G n ctt Leu 115 gt c Val gcg Al a t cc Ser t ac Tyr t ac Tyr 195 cac Hi s cag G n gac Asp cgt Ar g gac Asp gtt Val 100 ct g Leu at t SIle cgc Ar g agg Ar g ct c Leu 180 gga Gy at c SIle gtt Val at c SIle gac Asp gac Asp gat Asp act Thr ttg Leu at g Met cgc Ar g 165 gct Al a att SIe aac Asn gaa G u acc Thr 245 gcc acc gcc att gaa gtt gca ggt cca Ala Thr Ala Ile G u Val Ala y Pro 250 255 <210> 600 <211> 257 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 600 Val Asn Thr Asn Pro Ser G u Phe Ser Ser Asn Arg Ser Thr Ala Leu 1 5 10 Leu Thr Asp Lys Tyr G u Leu Thr IVet Leu G n Ala Ala Leu Ala Asp 25 G y Ser Ala G u Arg Pro Ser Thr Phe G u Val Phe Ser Arg Arg Leu 40 Page 748 seqI i st car r ect ed. t xt Pr o As n (31u Ar g Ar g Tyr G31y Val Val Ala G31y Thr Ala Ar g Val Leu Ly s As p Thr Ser (31u A a 145 vb t A a Tyr Le u Ly s 225 Thr Pr o I Ie Le u O31n Le u 115 ValI A a Ser Tyr Tyr 1 95 Hi s O31n As p As p As p As p Thr Le u vb t Ar g 1 65 A a I Ie As n O31u Thr 245 ValI Thr Tyr Ar g I Ie 135 Thr Hi s Ph e A a As p 215 Le u G31y Ph e Le u Ar g G31y 120 vb t A a O31u Ser Ser 200 G31y G31y ValI Thr O31u O31u 1 05 Thr As n A a Tyr Thr 1 85 G31y Thr ValI A a O31u 75 Le u O31u A a As p G31y 1 55 A a Ser Ser As n Thr 235 A a Le u As n Tyr cys 125 A a Pr o Thr Le u Hi s 205 A a Le u O31u A a Tyr Ph e 110 ValI ValI I Ie A a G u 1 A a A a Le u ValI As p Ar g Pr o I Ie A a I Ie Ser 1 A a Tr p A a ValI A a 255 Le u Ph e O31n Le u Ser (31u 1 Ar g A a Thr Ph e As p 240 G31y <210O> 601 <21 1> 509 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (486) <223> FRXA02754 <400> 601 ccc aca aac acc aag at t gt ggt ct cc tcc gac ctg gat gaattc gcc Pr o Thr Asn Thr Lys I Ie Val Val Ser Ser Asp Leu Asp O31u Phe Ala 1 5 10 at c gcg ggt ct t cgc ggc gaa cca gt t gac g tc t tt ggc gt t ggc acc Ilie Ala G31y Leu Ar g 31y O31u Pr o Val Asp Val Phe G31y Val G31y Thr 25 t cc gt t gt c aca ggt t ct ggc gca cca acc gct ggc ct c gt g tac aag Ser Val Val Thr G31y Ser G31y Ala Pr o Thr Ala G31y Leu Val Tyr Lys 40 at c ggg gaa gt t gcc ggt cac cct gt g gcc aag cgt t cc cga aac aag Page 749 seql i st cor r ect ed. t xt IIe Gy G u Val Ala G y His Pro Val Ala Lys Arg Ser Arg Asn Lys 55 gaa agc tac ggt ggt ggc aag aag gct gtg cgc acc cac cgc aag tcc 240 Su Ser Tyr Gy Gy G y Lys Lys Ala Val Arg Thr His Arg Lys Ser 70 75 ggt acc gca atc gaa gaa atc gtc tac cca ttc aat gcc gaa gca cca 288 Sy Thr Ala Ile u u Ile Val Tyr Pro Phe Asn Ala u Ala Pro 90 gat act gga aag ctc gac act ttg agc ctg acc atc cca ttg atg cgc 336 Asp Thr y Lys Leu Asp Thr Leu Ser Leu Thr lie Pro Leu IVet Arg 100 105 110 gac ggt gaa atc gtt cca ggt ttg cct act ttg gaa gat tcc cga gcg 384 Asp y G u Ile Val Pro y Leu Pro Thr Leu G u Asp Ser Arg Ala 115 120 125 tat ttg gcc aag caa ttg gtc tct tta cca tgg gaa ggc ctt gca ctg 432 Tyr Leu Ala Lys G n Leu Val Ser Leu Pro Trp G u y Leu Ala Leu 130 135 140 tct cgc gat gag cct gtt ttg cac act cgt ttc gtg ggt ttc ccg ccg 480 Ser Arg Asp G u Pro Val Leu His Thr Arg Phe Val G y Phe Pro Pro 145 150 155 160 gcc gct tagacaattc ggtctcacca aac 509 Al a Al a <210> 602 <211> 162 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 602 Pro Thr Asn Thr Lys Ile Val Val Ser Ser Asp Leu Asp G u Phe Ala 1 5 10 Ile Ala y Leu Arg Gy u Pro Val Asp Val Phe y Val y Thr 25 Ser Val Val Thr y Ser y Ala Pro Thr Ala y Leu Val Tyr Lys 40 IIe G y G u Val Ala G y His Pro Val Ala Lys Arg Ser Arg Asn Lys 55 G u Ser Tyr G y G y G y Lys Lys Ala Val Arg Thr His Arg Lys Ser 70 75 Sy Thr Ala Ile Gu Gu Ile Val Tyr Pro Phe Asn Ala u Ala Pro 90 Asp Thr y Lys Leu Asp Thr Leu Ser Leu Thr lie Pro Leu IVet Arg 100 105 110 Asp y G u Ile Val Pro y Leu Pro Thr Leu G u Asp Ser Arg Ala 115 120 125 Tyr Leu Ala Lys G n Leu Val Ser Leu Pro Trp G u y Leu Ala Leu 130 135 140 Ser Arg Asp G u Pro Val Leu His Thr Arg Phe Val G y Phe Pro Pro 145 150 155 160 Page 750 seal i st cor r ect ed. t xt Al a Al a <210> 603 <211> 960 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(937) <223> RXA02112 <400> 603 tgaatccgtg gcggcaccgg cgcgagtcgc tttggaaagg agctcctgtt actcctagct cctcgaagga tgcgtaattt atgctatctg ttgttccagc at g act lMt Thr acc cat att Thr His Ile gac Asp at t SIle gtt Val gcc Al a gct Al a ggc Gy at t l Ie gag G u ccc Pr a ttt Phe 150 cat Hi s tcg Ser at c SIle t cc Ser gcc Al a ttc Phe ggt Gy gct Al a agg Ar g aaa Lys 120 ct g Leu cac Hi s gca Al a at g M\et ggc G y act Thr gag G u ct c Leu agc Ser agc Ser t cc Ser acc Thr at c SIle gcc Al a at c l Ie 170 gct Al a gca Al a ttt Phe cca Pr a gt c Val ttt Phe 75 at c SIle ggc G y aaa Lys at t SIle acc Thr 155 gca Al a aaa Lys gcg Al a at c SIle ggt Gy gat Asp gaa G u ct c Leu at c SIle gcc Al a gaa G u 140 ttg Leu t cc Ser ct c Leu tta Leu cca Pr a gt g Val 45 cct Pr a acc Thr cgt Ar g gct Al a cgc Ar g 125 cgc Ar g t cc Ser cag G n ccc Pr a t cc Ser gga Gy ttc Phe agg Ar g ggg Gy t ca Ser aca Thr 110 at t SIle caa G n gat Asp ggg G y cac Hi s 190 gag G u 15 tcg Ser agc Ser at a SIle gac Asp gag G u 95 ttg Leu gtt Val gct Al a gct Al a ct c Leu 175 acc Thr gat Asp gcg Al a ggg Gy aac Asn at c SIle 80 cgc Ar g aca Thr gat Asp gt c Val gt c Val 160 agc Ser acc Thr 1 gcg Al a cag G n cag G n gca Al a ct a Leu at t SIle tcg Ser acc Thr cgt Ar g 145 at g lbt at c SIle cat Hi s cca Pr a ct g Leu gcg Al a t cc Ser gga Gy gct Al a tgc Cys cgg Ar g 130 gac Asp gt g Val act Thr gt g Val tgg Tr p agc Ser ctt Leu ctt Leu aca Thr ct c Leu t at Tyr 115 aaa Lys ggt G y aaa Lys gaa G u gaa G u 195 ggc G y gcc Al a tta Leu aag Lys at t SIle aac Asn 100 gtt Val acc Thr ggc G y gat Asp gcg Al a 180 gt c Val gac Asp aag Lys gac Asp gtg Val acc Thr ttc Phe gca Al a aca Thr gga G y aac Asn 165 ctg Leu gaa G u Page 751 gt t ValI ac c Thr g at As p 230 ct c Le u gt t ValI at t I Ie seqI i st car r ect ed. t xt cat ata gag cag at c gaa cca gtt ct t gct gct Hi s IlIe (1u (1n I Ie (1u Pr o Val LeuAia Aia 200 205 210 at g t tg gat aat t tc acc at t gat cag ct c at c I'b t Leu Asp Asn Phe Thr I I e Asp 03 n Leu I I e 220 225 at t ggt gga cgt gca ct g gt g gaa gca t ct ggc Ilie 31y G31y Ar g Ala Leu Val O31u Ala Ser G31y 235 240 acc gcg gga aag att gca tca acc ggt gtc gac Thr Ala G31y Lys Ilie Ala Ser Thr G31y Val Asp 250 255 gcg ct t acc cat t ct gt g cat gca ct t gac ct a Ala Leu Thr Hi s Ser Val Hi s Ala Leu Asp Leu 265 270 t aat gct ct a cct t gat aat gca ggt G31y g aa O31u gga G31y gt c ValI gga G31y 275 gt g ValI ggc G31y gt c ValI at t I Ie 260 ct c Le u <210O> 604 <21 1> 279 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 604 I'bt Thr Thr His IlieAsp Arg IlieVal A a O31n O31n A a Le u I Ie Ser Thr Ar g 145 vb t I Ie Tr p Ser Le u Le u Thr Le u Tyr 115 Ly s G31y Ly s O31u As p Ly s As p ValI Thr Ph e A a Thr G31y As n 1 65 Le u I Ie ValI A a A a 70 G31y I Ie O31u Pr o Ph e 150 Hi s Ser Thr ValI Ser As p Ser O31n ValI G31y 135 As n Le u As n Ser A a 40 Ph e G31y A a Ar g Ly s 120 Le u Hi s A a vb t As p Ar g Ar g As p Ar g Thr 1 G31y Ar g Ar g A a Ly s 1 85 G31y 10 Thr (31u Le u Ser Ser 90 Ser Thr I Ie A a I Ie 1 70 A a A a Leu Ser 03 u Asp Ph e Pr o ValI Ph e 75 li1e G31y Ly s I Ie Thr 1 55 A a Pr o ValI Pr o Thr Ar g A a Ar g 125 Ar g Ser O31n Ser Ser I Ie As p O31u Le u ValI A a A a Le u 1 A a O31y As n I Ie Ar g Thr As p ValI ValI 1 Se r Ala Lys Leu Pro Hi s Thr Thr 1 Page 752 Hi s A a Le u 225 Ser ValI As p O31u 1 95 G31y O31u G31y ValI G31y 275 O31u As p ValI As n 245 Ser As p As p I Ie 215 Le u As n G31y Ph e seql i st car r ect ed. t xt His lle(3u On lle(3u Pro Val Leu 200 205 l'b t Leu Asp As n Phe Thr I Ie Asp O31n 220 li1e 31y G31y Ar g Ala Leu Val O31u Ala 235 240 Thr A a G3 y Lys I I e A a Ser Thr G3 y 250 255 Ala Leu Thr Hi s Ser Val Hi s Ala Leu 265 270 <210O> 605 <21 1> 1407 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1384) <223> RXA02111 <400> 605 gcttgcggga acaccgcacc gcccacccaa actgttcaga gct cat tcca gcccaccgt t t agaagaaaa gaccccaat c t tccaaagat aaat tct gac at g acc acc t ca at c 11! l'bt Thr Thr Ser I Ie ac c Thr g aa O31u gt c ValI ccg Pr a g aa O31u gt g ValI gca A a cga Ar g t ct Ser aaa Ly s ggc O31y c ag O31n cat Hi s at c I Ie tt t Ph e gag O31u 120 aac As n gga O31y ggc O31y gt t ValI ag g Ar g gga O31y ggt O31y g aa O31u gca A a t gg Tr p t ca Ser ccc Pr a cgg Ar g tt c Ph e t ct Ser at t I Ie tt g Le u tt c Ph e c aa O31n 45 gag (31u g aa (31u t ac Tyr tt c Ph e gt g ValI 125 aaa Ly s ct c Le u aac As n gag (31u gcg A a c ag O31n c aa O31n 110 tt c Ph e aat As n 15 g ac As p g at As p t ac Tyr aaa Ly s cgc Ar g 95 ct t Le u t gc Cys gcc A a c ag O31n ccg Pr a c ag O31n g ac As p 80 g at As p gcc A a ggt O31y ag c Ser gga O31y cct Pr a 50 gca A a ct g Le u gt t ValI gct A a c ac Hi s 130 t gc cys at g vb t 35 gcg A a agt Ser ggt O31y at c I Ie gcc A a 115 tt c Ph e aac As n 20 ccg Pr a cat Hi s g at As p g ac As p c aa O31n 1 00 aaa Ly s at g vb t agt Ser g at As p gct A a g ac As p aaa Ly s c ac Hi s ac c Th r gct A a Page 753 gaa acc gct gat G u Thr Ala Asp 135 ctg tta tcc acg Leu Leu Ser Thr eql i st cor r ect ed. t gat gaa caa t ca Asp G u n Ser gtg at c ctc ccc Val Ile Leu Pro aac Asn 150 gt c Val ct g Leu gt g Val gt a Val gat Asp 230 gat Asp gtt Val tct Ser gag G u gtt Val 310 gcc Al a at c SIle ttc Phe cac Hi s gt g Val 390 gcc Al a gac Asp cct Pr o gag G u 200 gag G u cac Hi s at g let gag G u cac Hi s 280 ccc Pr o gac Asp caa G n ttg Leu ct c Leu 360 ggt Gy aac Asn ggt Gy tgg Tr p 170 acc Thr ggc Gy gcg Al a ggt Gy ct g Leu 250 gaa G u cgc Ar g gtt Val gcc Al a gca Al a 330 cag G n cct Pr o ct g Leu at t SIle tgc Cys 155 gag G u tac Ty r gga Gy ttt Phe cga Ar g 235 tgg Tr p aac Asn ttt Phe cac Hi s gac Asp 315 ccg Pr o cgc Ar g gt c Val gcc Al a tct Ser 395 140 t cc Ser caa G n at g Met at t SIle gaa G u 220 aac Asn aat Asn gca Al a act Thr gt c Val 300 t ca Ser gca Al a ct g Leu at c SIle tgg Tr p 380 gt c Val at g NMt ct c Leu aat Asn gt a Val 205 cgc Ar g acc Thr ccc Pr o aag Lys gt c Val 285 at c SIle t cc Ser gga Gy gca Al a tgc Cys 365 gca Al a tct Ser gca Al a acc Thr t cc Ser 190 tgc Cys ggc Gy gcg Al a aac Asn gt a Val 270 gag G u gt g Val gga Sy tct Ser gcc Al a 350 cca Pr o ctt Leu gaa G u gac Asp t ca Ser 175 tct Ser acc Thr caa G n aaa Lys aaa Lys 255 ct g Leu cag G n cac Hi s t cc Ser acc Thr 335 cag G n tgc Cys gag G u t cc Ser at g NMt 160 at t SIle gca Al a t cc Ser cga Ar g gcc Al a 240 cca Pr o ct c Leu at c SIle cct Pr o act Thr 320 ttt Phe t ac Tyr t cc Ser gag G u gt g Val 400 gct Al a t at Tyr gcg Al a t ca Ser gt c Val 225 at g Met ct g Leu tgg Tr p aac Asn gaa G u 305 gac Asp gcg Al a ccg Pr o acc Thr ttg Leu 385 gcg Al a gac Asp ggc G y ct c Leu aat Asn 210 ct g Leu ggc Gy ggt Sy cat Hi s aaa Lys 290 t cc Ser ttc Phe at c l Ie cag G n at g Met 370 gt g Val gca Al a ctt Leu gat Asp aaa Lys 195 gca Al a ttc Phe at t SIle ggc Sy ggt Sy 275 gcc Al a ccc Pr o at t SIle ggc G y cac Hi s 355 t at Tyr gct Al a ccg Pr o gat Asp gac Asp 180 ggt G y cgt Ar g ttc Phe ggg Gy aac Asn 260 ttc Phe cgc Ar g at g Met gt g Val acc Thr 340 acc Thr cgc Ar g gga G y gcg Al a 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 Page 754 seqI i st car r ect ed. t xt gt c gct t tg gaa agg at g cta tct gtt gtt cca gca gct cct gtt act Val Al a Leu O31u Ar g I'bt Leu Ser Val Val Pro Ala Ala Pro Val Thr 410 415 420 cct agc t cc t cg aag gat gcg t aat t tat ga ct acccat at t ga Pr o Ser Ser Ser Lys As p Al a 425 <210O> 606 <21 1> 428 <21 2> PRT <21 3> Cor ynebact er i urn gl ut anri cumn 1363 1407 <400> 606 I'bt Thr Thr Ser Asn Ser Cys Asn Pro O31y I'bt Pr o li1e Pro Al a Hi s Ar g Al a Ser As p Thr Leu O31y As p O31u Val I Ie O31n 1 00 Ar g Al a Al a Lys 115 Val Hi s Phe I'b t 130 Ser Val I Ie Leu 145 Al a Asp Leu Asp Tyr 03 y Asp Asp 1 80 Al a Leu Lys 03 y 1 95 Ser Asn Al a Ar g 210 Val Leu Phe Phe 225 b t O31y Ilie (31y Leu 03 y 03 y Asn 260 Tr p Hi s (3y Phe 275 le Ser As p Al a As p Lys5 Hi s Thr Al a Pr a Ser 1 65 Thr Ph e Ser Pr a I Ie 245 Thr Cys Pr a Le u Tyr Ar g 55 Le u ValI As p Pr a Thr 135 Le u O31u I Ie O31y Le u 215 O31n O31n Ser ValI Ser Lys5 G31y 40 O31n Hi s I Ie Ph e O31u 120 Al a Al a As p Pr a O31u 200 O31u Hi s vb t O31u Hi s 280 Val Asn 10 As p (1y 25 Pro O31y O3 n Val Ar g Ar g Leu 03 y 90 Val O31y 1 05 Al a 03 u Asp Leu Al a 03 y Cys Tr p 1 70 Val Thr 1 85 Hi s 03 y Tr p Al a Leu 03 y Pro Leu 250 Leu 03 u 265 Lys Ar g Leu Al a Leu Lys As n Al a Pr a Al a Le u I Ie 75 Hi s As p Al a Le u Cys 1 55 O31u Tyr O31y Ph e Ar g 235 Tr p As n Tr p Ser Pr a Ar g Ph e Ser I Ie Ser 140 Ser O31n vb t I Ie O31u 220 As n As n Al a Ph e O31n O31u O31u Tyr Ph e ValI 125 Thr vb t Le u As n ValI 205 Ar g Thr Pr a Lys5 Le u As n O31u Al a O31n O31n 110 Ph e As p Al a Thr Ser 1 Cys O31y Al a As n ValI 270 As p As p Tyr Lys5 Ar g Le u Cys O31u As p Ser 1 Ser Thr O31n Ly s Ly s 255 Le u O31n Pr a O31n As p As p Al a O31y O31n vb t 1 li1e Al a Ser Ar g Al a 240 Pr a Le u Phe Thr Val cOu On Ilie 285 Page 755 Asn G u 305 Asp Al a Pr o Thr Leu 385 Al a Al a Al a Pr o SIle Gy Hi s 355 Tyr Al a Pr o Pr o Al a Pr o Lys 325 G u SIle SIle Asn Ar g 405 Thr G u Val 310 Al a SIle Phe Hi s Val 390 Val Pr o Tyr 295 Val l Ie Asn Cys Pr o 375 I le Al a Ser seql i st cor r ect ed. t xt Pro Asp Val His Val li e 300 Asp Ala Ala Asp Ser Ser 315 Sn Ala Ala Pro Ala y 330 Leu Val G n Arg Leu Ala 345 Leu Asp Pro Val lie Cys 360 365 Sy Tyr Leu Ala Trp Ala 380 Asn n lie Ser Val Ser 395 Leu G u Arg IVet Leu Ser 410 Ser Ser Lys Asp Ala 425 Hi s Ser Thr 335 G n Cys G u Ser Val 415 <210> 607 <211> 954 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(931) <223> RXA01073 <400> 607 taaccgactc cagcactaaa ctccaaaccc ttggcccgca ccaagacacc accgcgccat gtttgcctag gattaggtac acc Thr aag Lys t cc Ser act Thr gaa G u gcg Al a at c SIle gtt Val aca Thr gcg Al a aac Asn cag G n aat Asn ttc Phe ggc Sy cga Ar g acg Thr gat Asp gaa G u cgt Ar g ttt Phe ct c Leu gat Asp 75 gag G u ct a Leu gt g Val gtt Val acg Thr t at Tyr gac Asp gt g Val ttc Phe ggt Sy ct g Leu ttc Phe gcg Al a agc cca Ser Pro 15 ct c gt c Leu Val att tca Ile Ser gca gt a Al a Val tac gca Tyr Al a 80 caa gtt G n Val Page ccgccaaagt ttagcgcgcc atg aca aac act caa IVet Thr Asn Thr G n 1 gca atc gac gtg gcc Ala lie Asp Val Ala gat tac ctg cgg gct Asp Tyr Leu Arg Ala ggt ggc cag gat tcc G y G y G n Asp Ser gag cgc att cgt gcg Su Arg lie Arg Ala gtt cgc ctc ccc tac Val Arg Leu Pro Tyr gca ttg gaa ttc atc Ala Leu G u Phe Ile seqI i st car r ect ed. t xt gca A a ac c Thr t tc Ph e gca A a 1 50 gct A a g ac As p ct g Le u gct A a ggt G31y 230 gt c ValI c ag O31n g ac As p gca A a 120 cgg Ar g gca A a aac As n ct t Le u c ac Hi s 200 t tg Le u t cg Ser g aa O31u aag Ly s ag c Ser gt t ValI aat As n c ag O31n ac g Thr 1 70 t tg Le u ggt O31y gag O31u gcg A a gcc A a 250 c ac Hi s gt g ValI gca A a at t I Ie t t g Le u 155 ggg O31y gca A a gca A a g at As p gac As p 235 cag O31n ct c Le u ac c Thr gct A a aaa Ly s 140 ggc G31y tt c Ph e ggt G31y cct Pr o cgc Ar g 220 at c I Ie c ag O31n cct Pr o gt c ValI gct A a 125 gct A a tt g Le u tt c Ph e tt g Le u t ca Ser 205 cca Pr o g at As p cgc Ar g aac As n 110 tt g Le u cgc Ar g ct g Le u ac c Thr ag c Ser 1 90 ag c Ser gcg A a aat As n at t I Ie gt t ValI g aa O31u c aa O31n gt t ValI aaa Ly s 1 75 aag Ly s ac g Thr tt g Le u t ac Tyr gag O31u 255 aaa Ly s ct t Le u cgc Ar g at t I Ie 1 60 tt c Ph e cgt Ar g t gg Tr p cca Pr o ct g Le u 240 c ac Hi s gca A a gag O31u 130 gt t ValI act Thr g at As p gga O31y aag Ly s 210 gag O31u aac As n t gg Tr p ac g Thr 115 ct g Le u gcc A a g at As p ggc O31y gct A a 1 gt t ValI g aa O31u aag Ly s aag Ly s g ac As p ac c Thr c ag O31n c ac Hi s gca A a 1 gcc A a cct Pr o gca A a ccc Pr a gt g ValI 260 gcc A a g ac As p t ac Tyr gcg A a 1 gct A a at t I Ie ac c Thr ct t Le u g at As p 245 ggc O31y gct acc ccg cag gaa aat Ala Thr Pro On Ou Asn 270 t gg t gg cgt Trp Trp Arg 275 t aat ccaaca gt t tgagt gt cgc <210O> 608 <21 1> 277 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 608 I'bt Thr Asn Thr On Thr 0u Ilie Ilie 1 5 Ala I Ie Asp Val Ala Lys O31u Val O31u 25 Asp Tyr Leu Arg Ala Se r Hi s Th r Lys 40 O31y O31y O31n As p Ser Thr Leu Ala O31y 55 O31u Ar g I Ie Ar g Ala O31u O31u As n Ser Asn (31u Leu Lys Val Ser Pro 10 Phe Ar g Val O31n Phe Leu Val (31y Phe Val Leu O31y I Ie Ser Ar g Leu Thr O31n Leu Ala Val Thr Asp Tyr Val Phe Tyr A a Page 757 seql i st cor r ect ed. t xt Al a l e G n Ala G u Asp Asp Al a Val Al a Asp Pr o let 145 Gy Gy G n Thr Asp 225 G u Leu G u Leu G u Thr 115 Leu Al a Asp Gy Al a 195 Val G u Lys Lys Tr p 275 Tyr I e Al a Asp Tyr Al a 165 Al a l Ie Thr Leu Asp 245 Gy Ar g Al a Thr Phe Al a 150 Al a Asp Leu Al a Gy 230 Val G n Pr o G u Asn 135 l le G u Leu G u Asp 215 Val Ser Hi s Asp Al a 120 Ar g Al a Asn Leu Hi s 200 Leu Ser G u Lys Lys 105 Thr Gy Gy Val Pr o 185 Leu G u Tyr Lys Ar g 265 Asp 90 Ser Val Asn G n Thr 170 Leu Gy G u Al a Al a 250 Hi s Val Al a SIle Leu 155 Gy Al a Al a Asp Asp 235 G n Leu Thr Al a Lys 140 Sy Phe Sy Pr o Ar g 220 I le G n Pr o Val Al a 125 Al a Leu Phe Leu Ser 205 Pr o Asp Ar g Al a G n Val G u G n Val Lys 175 Lys Thr Leu Tyr G u 255 Pr o <210> 609 <211> 1461 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1438) <223> RXN02754 <400> 609 attatgaagc catcggagtt ggtgtggcct acaagggtga tcatgcgtgg atagtggtgg agt t cactgt agctcccgct gat t ccgtag aatcaacaga gtg aat acc aat ccg Val Asn Thr Asn Pro 1 tct gaa ttc tcc tca aac cgt tca aca gct ctc ctt act gat aaa tat Ser G u Phe Ser Ser Asn Arg Ser Thr Ala Leu Leu Thr Asp Lys Tyr 15 gag ctg acc atg ctt caa gca gcg ctc gct gat ggt tct gca gaa cgc G u Leu Thr IVet Leu G n Ala Ala Leu Ala Asp y Ser Ala G u Arg 30 ccc tca acg ttt gag gtc ttt agc cgc cgc ctc ccc aac gag cgc cga Page 758 s Pro Ser Thr Phe G u Val Phe Ser 45 eql i st cor r ect ed. t xt Arg Arg Leu Pro Asn G u Arg Arg t ac Tyr ttt Phe cgt Ar g ggc Gy gt g Val t cc Ser gt c Val 150 acc Thr gga G y cca Pr o gat Asp t cc Ser 230 caa G n gt a Val aag Lys t cc Ser gt c Val ttc Phe ct g Leu cgc Ar g ggc Gy 120 at g let gca Al a gaa G u t cc Ser t cc Ser 200 ggc G y ggc Gy gt g Val at c SIle ct c Leu 280 ct g Leu gca Al a gag G u t ac Tyr ggc Gy ttt Phe gca Al a gat Asp tcg Ser 170 acc Thr acc Thr ccc Pr o gac Asp acc Thr 250 t cc Ser gat Asp gaa G u gga G y gaa G u 75 ct c Leu gaa G u gca Al a gat Asp ggt G y 155 gca Al a t cc Ser t cc Ser aac Asn acc Thr 235 gcc Al a ggc Gy ct c Leu tt c Phe aca Thr caa G n cgc Ar g at c SIle gaa G u tct Ser 140 cgc Ar g gt c Val aac Asn gcc Al a gaa G u 220 acc Thr at t l Ie gac Asp aac Asn gcc Al a 300 gca Al a ct c Leu aac Asn t ac Tyr tgc Cys 125 gcc Al a ccc Pr o acc Thr ct c Leu cac Hi s 205 gca Al a ttg Leu gaa G u ct a Leu gcc Al a 285 at c l Ie cga Ar g gcc Al a t ac Tyr ttc Phe 110 gt c Val gt c Val at c SIle gca Al a gag G u 190 gca Al a gca Al a ct g Leu gtt Val ggt Gy 270 cac Hi s gcg Al a gt g Val gat Asp cga Ar g 95 ccg Pr o at c SIle gct Al a at c SIle t cc Ser 175 gcg Al a tgg Tr p gct Al a gt a Val gca Al a 255 gt g Val aac Asn ggt Gy ct g Leu ctt Leu ttc Phe cag G n ct a Leu t cc Ser gaa G u 160 cgc Ar g gcc Al a act Thr ttc Phe gat Asp 240 ggt Gy ctt Leu acc Thr ctt Leu aag Lys gac Asp acc Thr t cc Ser gaa G u gcc Al a 145 at g Met gca Al a t ac Tyr ttg Leu aaa Lys 225 act Thr cca Pr o gcc Al a aag Lys cgc Ar g 305 gcg Al a ttt Phe ggc Gy cct Pr o act Thr 130 gct Al a gga G y gca Al a cgc Ar g ct g Leu 210 gca Al a t at Tyr gac Asp cga Ar g at t SIle 290 ggc Gy at t l Ie tta Leu caa G n ctt Leu 115 gt c Val gcg Al a t cc Ser t ac Tyr t ac Tyr 195 cac Hi s cag G n gac Asp ctt Leu aag Lys 275 gt g Val gaa G u cgt Ar g gac Asp gtt Val 100 ct g Leu at t SIe cgc Ar g agg Ar g ct c Leu 180 gga G y at c SIe gtt Val at c SIe ggt Gy 260 gt c Val gt c Val cca Pr o 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 gac gtc ttt ggc gtt ggc acc tcc gtt gtc aca ggt tct ggc gca cca Page 759 Asp 310 Val Phe G y Val seql i st cor r ect ed. t xt G y Thr Ser Val Val Thr y Ser 315 320 G y Al a Pro 325 acc gct ggc ctc gtg tac aag atc ggg Thr Ala Gy Leu Val Tyr Lys Ile y 330 gcc aag cgt tcc cga aac aag gaa agc Ala Lys Arg Ser Arg Asn Lys u Ser 345 350 gtg cgc acc cac cgc aag tcc ggt acc Val Arg Thr His Arg Lys Ser G y Thr 360 365 cca ttc aat gcc gaa gca cca gat act Pro Phe Asn Ala G u Al a Pro Asp Thr 375 380 ctg acc atc cca ttg atg cgc gac ggt Leu Thr lie Pro Leu IVet Arg Asp y 390 395 act ttg gaa gat tcc cga gcg tat ttg Thr Leu G u Asp Ser Arg Ala Tyr Leu 410 cca tgg gaa ggc ctt gca ctg tct cgc Pro Trp G u G y Leu Al a Leu Ser Ar g 425 430 cgt ttc gtg ggt ttc ccg ccg gcc gct Arg Phe Val Gy Phe Pro Pro Ala Ala 440 445 aac <210> 610 <211> 446 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 610 Val Asn Thr Asn Pro Ser u Phe Ser 1 5 Leu Thr Asp Lys Tyr G u Leu Thr IVet Sy Ser Ala G u Arg Pro Ser Thr Phe 40 Pro Asn G u Arg Arg Tyr y Val Val 55 Lys Ala lie Arg Asp Phe Val Phe Thr 70 Asp Phe Leu Asp Asp Arg Thr Leu G u Thr Gy n Val Asp y Tyr Arg u 100 105 Ser Pro Leu Leu Thr Val Arg Gy Thr 115 120 gaa Gu 335 t ac Tyr gca Al a gga Gy gaa Gu gcc Al a 415 gat Asp gtt Val ggt Gy at c l Ie aag Lys at c SIle 400 aag Lys gag G u gcc Al a ggt Gy gaa Gu ct c Leu 385 gtt Val caa G n cct Pr o ggt G y ggc Gy gaa G u 370 gac Asp cca Pr o ttg Leu gtt Val cac cct His Pr o 340 aag aag Lys Lys 355 at c gt c Ile Val act ttg Thr Leu ggt ttg G y Leu gt c t ct Val Ser 420 ttg cac Leu Hi s 435 gt g Val gct Al a t ac Tyr agc Ser cct Pr o 405 tta Leu act Thr 1123 1171 1219 1267 1315 1363 1411 1458 1461 tagacaattc ggtctcacca Ser Asn Arg 10 Leu G n Al a G u Val Phe Al a G y Thr G u G u G n Tyr Leu Arg 90 G y u lie Phe Al a G u Page 760 Ser Al a Ser Al a Leu Asn Tyr Cys 125 Al a Al a Ar g Val Asp Ar g Pr o SIe seql i st cor r ect ed. t xt G u Thr Val lie Leu Ser lie IVet Asn Ala Asp Ser Al a Val Ala Ser 130 135 Al a 145 Met Al a Tyr Leu Lys 225 Thr Pr o Al a Lys Ar g 305 Gy Al a Gy G u Leu 385 Val G n Pr o Al a Ser Tyr Tyr 195 Hi s G n Asp Leu Lys 275 Val G u Gy Hi s Lys 355 I le Thr Gy Val Leu 435 lbet Ar g 165 Al a SIle Asn G u Thr 245 Gy Ar g Ser Val Pr o 325 Val Al a Tyr Ser Pr o 405 Leu Thr Thr Hi s Phe Al a Asp 215 Leu Gy Ar g G n Asp 295 Val Al a Lys Ar g Phe 375 Thr Leu Tr p Phe Al a G u Ser Ser 200 Gy Gy Val SIle Leu 280 Leu Phe Gy Ar g Thr 360 Asn SIle G u G u Val 440 Al a Tyr Thr 185 Gy Thr Val Al a Asp 265 Asp Asp Gy Leu Ser 345 Hi s Al a Pr o Asp Gy 425 Gy Gy 155 Al a Ser Ser Asn Thr 235 Al a Gy Leu Phe Gy 315 Tyr Asn Lys Al a let 395 Ar g Al a Pr o 140 Ar g Val Asn Al a G u 220 Thr l Ie Asp Asn Al a 300 Thr Lys Lys Ser Pr o 380 Ar g Al a Leu Pr o Pr o Thr Leu Hi s 205 Al a Leu G u Leu Al a 285 l le Ser SIle G u Gy 365 Asp Asp Tyr Ser Al a 445 SIle Al a G u 190 Al a Al a Leu Val Gy 270 Hi s Al a Val Gy Ser 350 Thr Thr Gy Leu Ar g 430 Al a SIe Ser 175 Al a Tr p Al a Val Al a 255 Val Asn Gy Val G u 335 Tyr Al a Gy G u Al a 415 Asp <210> 611 <211> 531 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS Page 761 seql i st cor r ect ed. t xt <222> (101)..(508) <223> RXA02299 <400> 611 acgcgggggt tgttgccgga tcgaaatatt cctttccttg taaaacttgc aggacaaccc ccataaggac accacaggac tcatctcacg ctatgatttc ctc gga agt aag att cac cga gcc act g Leu G y Ser Lys lie His Arg Ala Thr V tat gtt ggc tct gta acc atc gac gcc g Tyr Val y Ser Val Thr lie Asp Ala A ttg atc gaa ggc gaa aaa gtt gcc atc g Leu Ile u G y u Lys Val Ala Ile V 45 cgt ctg gaa act tat gtc att gtg ggc g Arg Leu G u Thr Tyr Val Ile Val Gy A 60 tgc atc aat ggt gcc gct gca cac ctt a Cys lie Asn G y Al a Al a Al a Hi s Leu I 75 atc atc atg agc tac ctt cag gca act g lie lie IVet Ser Tyr Leu n Ala Thr A gag cca aag att gtg cac gtg gac gcc g Su Pro Lys Ile Val His Val Asp Ala A 105 110 ggc aac gat ctt gcg gaa gca cta cct g Sy Asn Asp Leu Ala G u Ala Leu Pro C 120 125 aga agc att tagcgtttta gctcgccaat att Arg Ser Ile 135 <210> 612 <211> 136 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 612 NIet Leu Arg Thr Ile Leu Gy Ser Lys I 1 5 G n Ala Asp Leu Asp Tyr Val y Ser V 25 Val His Ala Ala G y Leu I I e u G y C 40 Ile Thr Asn Gy Ala Arg Leu u Thr T 55 G y Thr G y Asn I e Cys I e Asn G y A 70 F tc 'al 15 ac sp ta 'al ac sp tt le at sp 95 ac sp ga Sy act Thr ct g Leu gac Asp gcc Al a aat Asn 80 gcg Al a aac Asn t cc Ser at g Met 1 caa G n gtt Val at c SIle gga Gy cct Pr o gaa G u cgc Ar g ggg Gy gct Al a cac Hi s acc Thr acg Thr ggc Gy gcc Al a at c SIle ctt Leu 130 ctg cgc acc atc Leu Arg Thr Ile ct a Leu gcc Al a ggc Gy aat Asn ctt Leu gcg Al a 100 gcg Al a acg Thr gat Asp gga Gy gct Al a att SIe gtg Val t at Tyr ct c Leu tcg Ser I e Hi s 10 'al Thr Su Lys yr Val I a Al a 75 'age 762 Arg Ala Thr Val Thr Ile Asp Ala Asp Leu Val Ala lle Val Asp Ile Val Gy Asp Ala Ala His Leu Ile Asn 2 seqI i st car r ect ed. t xt Pro Oy Asp Leu Val Ilie Ilie Ibt Ser Tyr Leu O31n Ala Thr Asp Ala 90 O31u Ala Lys Ala Ty r O31u Pro Lys I Ie Val His Val Asp Ala Asp Asn 100 105 110 Ar g I Ie Val Ala Leu (1y As nAs p Leu Ala(1u Ala Leu Pro (1y Se r 115 120 125 G31y Leu Leu Thr Ser Ar g Ser I Ie 130 135 <210O> 613 <21 1> 960 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (937) <223> RXA01928 <400> 613 gcgagt acgc cacct tgggc gat tcct tgc acgacgccgc gcaggcct ac at cgccgat a t ccacgcggg t acct tccca ggcgaagcgg agt cctt tt a at g cag gt a gca acc I'bt O31n Val Ala Thr ac a Thr ct c Le u aaa aat As n t at Tyr gt g ValI tt g Le u ggt G31y aag g at As p c ag O31n ccc Pr a gca A a ct g Le u cgc Ar g at t I Ie ct a Le u ag c Ser 120 tt c Ph e c ag O31n ct t Le u at g vb t gct A a tt t Ph e ct c Le u tt c Ph e t gg Tr p cct Pr a tt g Le u gt t ValI at c I Ie ggt O31y gaa O31u gaa O31u gac As p 75 gca A a gcg A a ggc O31y gt g ValI gcg A a g ac As p gcg A a aac As n gca A a gcc A a ccc Pr a cgc Ar g cat Hi s cgc Ar g 140 gt g ValI gcc A a ct a Le u g ac As p 45 ct c Le u g at As p g at As p ac c Thr tt c Ph e 125 cct Pr a at t I Ie ct c ct c Leu Leu 15 cac agc Hi s Ser act gt t Thr Val ggt gat 31y As p tta gca Leu A a gt g gag Val O31u 95 ggt t cc O3 y Ser 110 gat ggt As p (1y gat cgt Asp Arg cgg cga Ar g Ar g c ac Hi s gga O31y gt a ValI t gc cys ct g Le u g aa O31u at c I Ie gt g ValI gca A a 1 c ac Hi s c ac Hi s gcc A a g at As p ct t Le u at g vb t gga O31y gct A a t at Tyr 145 aaa Lys5 gcc A a agt Ser g at As p g aa O31u t ac Tyr ac a Thr ac c Thr 130 tt t Ph e t cc Ser t cg Ser at t I Ie t ac Tyr gag O31u ccc Pr a aaa Lys5 115 gt g ValI gga O31y gt c ValI tt g Le u tt t Ph e cgc Ar g gca A a ggt O31y 1 00 tt g Le u gt g ValI c aa O31n ttg gtt gcc gat cta gac Leu Val Ala Asp Leu Asp Page 763 150 at t SIle tta Leu caa G n gca Al a agc Ser 230 acc Thr gt g Val gag Gu seql i st cor r ect ed. t xt 155 160 gtg gag att cgt ccc gtt ccg att att cgt ggc Val u Ile Arg Pro Val Pro Ile Ile Arg y 170 175 gaa tcc agc cgc aat caa cgt ctt tct gcg gat Su Ser Ser Arg Asn G n Arg Leu Ser Ala Asp 185 190 ctg gtg ctg ccg cag gtg ttg agt ggg ttg cag Leu Val Leu Pro G n Val Leu Ser G y Leu G n 200 205 210 ggt gaa gcg cta gat atc caa ggt gcg cgc gac G y G u Al a Leu Asp lie On G y Ala Arg Asp 220 225 gac ggc gtg cgc ttg gat cac ctg gaa att gtc Asp G y Val Arg Leu Asp His Leu G u IIe Val 235 240 gaa cca tta gaa atc gac ggc ctg ctc acc caa G u Pro Leu G u Ile Asp y Leu Leu Thr G n 250 255 ggc gcg att ttc gtg ggg ccg gtg cgg ttg atc Sy Ala lie Phe Val y Pro Val Arg Leu Ile 265 270 t agt accaac cctgcgttgc agc gcc Al a cag G n 195 cgt Ar g acc Thr gat Asp cca Pr o gac Asp 275 gat Asp 180 cga Ar g cga Ar g ttg Leu cca Pr o gcg Al a 260 aat Asn 165 ggc G y gcg Al a aaa Lys gcc Al a gcc Al a 245 ttg Leu at c SIe <210> 614 <211> 279 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 614 Met G n Val Ala Thr Thr Lys G n Ala 1 5 His Lys Ser Val G y Leu Val Pro Thr His Ala Ser Leu Val Lys Ala Ala Arg 40 Ala Ser Ile Phe Val Asn Pro Leu G n 55 Asp Asp Tyr Arg Asn Tyr Pro Arg G n 70 Leu u u Ala y Val Asp lie Val IVet Tyr Pro y y Leu Pro Leu Val 100 105 Sy Thr Lys Leu G u G y Ala Ser Arg 115 120 Ala Thr Val Val Ala Lys Leu Phe Asn 130 135 Leu Ile Asp 10 Mvt y Al a Al a u Asn Phe G u Al a Leu Asp Al a Phe Al a Pr o 90 Tr p Al a Ar g Pro G y Hi s Leu Val Arg 140 Page 764 Al a Leu Asp Leu Asp Asp Thr Phe 125 Pr o Leu Ser Val Asp Al a G u Ser Sy Ar g Tyr 145 Val Ar g Al a Leu Ar g 225 I le Thr Leu Gy Asp Al a G n 195 Ar g Thr Asp Pr o Asp 275 Lys Asp 165 Gy Al a Lys Al a Al a 245 Leu SIle Al a Pr o Al a Al a Al a 215 Al a Leu Val Leu seql i st cor r ect ed. t xt Sn n Val Ala Val lie 155 Val u Ile Arg Pro Val 170 Su Ser Ser Arg Asn G n 185 Leu Val Leu Pro G n Val 200 205 G y G u Al a Leu Asp Il e 220 Asp y Val Arg Leu Asp 235 Su Pro Leu u Ile Asp 250 Sy Ala lle Phe Val y 265 Ar g SIe 175 Leu Ser Sy Leu Leu 255 Val <210> 615 <211> 936 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(913) <223> RXN01929 <400> 615 aaaatttgac tccataacga gaacttaatc gagcaacacc ggaatttatt tattctgagc tggtcatcac atctatactc at t SIle aac Asn cgc Ar g gct Al a gat Asp gcg Al a gca Al a cag G n ttt Phe aac Asn at g Met gt g Val aaa Lys gtt Val gag G u gt g Val gt g Val gtt Val cgc Ar g gtt Val ggc G y ggt G y gcc Al a ct g Leu cgt Ar g acc Thr gat Asp gat Asp gcg Al a ttt Phe cat ttc Hi s Phe 15 agc t at Ser Tyr at g ct c IVet Leu acc acc Thr Thr gtg acg Val Thr 80 ggt acc G y Thr 95 Page 765 cctgaacagt gaatcaaatc atg ccc atg tca ggc 115 IVet Pro IVet Ser y 1 cgc gaa gct aaa gta 163 Arg G u Ala Lys Val gat gcg ctt tcg gcg 211 Asp Ala Leu Ser Ala ctt gtt ggt gat tcc 259 Leu Val G y Asp Ser ttg tcg atc acc ttg 307 Leu Ser Ile Thr Leu atc gct acg aag cgt 355 lie Ala Thr Lys Arg tat gag gtg agc cca 403 Tyr G u Val Ser Pro 100 seql i st cor r ect ed. t xt aat cag gcg gtg gag tcc gcg atc cgg gtc atg cgt Asn G n Ala Val u Ser Ala lie Arg Val IVet Arg 105 110 gct gcg gtg aag atc gag ggt ggc gtg gag atc gcg Al a Ala Val Lys I le Gu G y Gy Val Gu Ile Ala 120 125 cgc att gtt gat gct gga att ccg gtt gtc ggc cac Arg lie Val Asp Ala y IIe Pro Val Val G y Hi s 135 140 145 ccg cag tcc gag cat tcc ttg ggc ggc cac gtg gtt Pro G n Ser G u His Ser Leu G y G y His Val Val 150 155 160 gcg agt tct gga aag ctc atc gcc gat gcc cgc gcg Ala Ser Ser y Lys Leu lie Ala Asp Ala Arg Al a 170 175 ggt gcg ttt gcg gtt gtg ttg gag atg gtt cca gca G y Ala Phe Ala Val Val Leu G u bet Val Pro Al a 185 190 gag gtt acc gag gat ctt tcc atc acc act atc gga Su Val Thr u Asp Leu Ser Ile Thr Thr Ile y 200 205 aat ggc aca gat ggg cag gtt ttg gtg tgg cag gat Asn y Thr Asp G y n Val Leu Val Trp G n Asp 215 220 225 aac cgc ggc aag aag cca cgc ttc gtc cgc gag tac Asn Arg y Lys Lys Pro Arg Phe Val Arg G u Tyr 230 235 240 gat tcc ttg cac gac gcc gcg cag gcc tac atc gcc Asp Ser Leu His Asp Al a Ala G n Ala Tyr Ile Ala 250 255 ggt acc ttc cca ggc gaa gcg gag tcc ttt taatgca Sy Thr Phe Pro Gy G u Ala u Ser Phe 265 270 aag <210> 616 <211> 271 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 616 IVet Pro IVet Ser Gy IIe Asp Ala Lys Lys Ile Arg 1 5 10 Arg G u Ala Lys Val Asn Gy G n Lys Val Ser Val 25 Asp Ala Leu Ser Ala Arg lie Phe Asp u Ala y 40 Leu Val y Asp Ser Ala Ala Asn Val Val Leu y 55 Leu Ser lie Thr Leu Asp G u Iet Ile Val Leu Ala 70 75 Page 766 gaa acg G u Thr 115 cag acg G n Thr 130 atc ggg lie G y cag ggt G n y ttg gag Leu G u gag gca G u Al a 195 atc ggt I e G y 210 gcc ttc Al a Phe gcc acc Al a Thr gat at c Asp Il e ggt G y at t SIle t ac Tyr cgt Ar g cag G n 180 gcg Al a gcc Al a ggc G y ttg Leu cac Hi s gcg Al a cga Ar g acc Thr ggc G y 165 gcg Al a cgc Ar g ggc G y ct c Leu ggc G y 245 gcg Al a 260 ggt agcaaccaca Thr Arg His Phe Leu Thr Ser Tyr Val Asp IVet Leu Arg Asp Thr Thr Lys Al a Val Thr seql i st cor r ect ed. t xt lie Ala Thr Lys Arg Al a Leu Val Val Val Asp Leu Pro Phe Tyr Ar g Al a Hi s 145 Val Al a Al a Gy Asp 225 Tyr Al a Val Thr 115 Thr Gy Gy G u Al a 195 Gy Phe Thr l Ie Pr o Al a Ar g Thr Gy 165 Al a Ar g Gy Leu Gy 245 Al a Asn Al a Ar g Pr o 150 Al a Gy G u Asn Asn 230 Asp Gy G n Al a SIle 135 G n Ser Al a Val Gy 215 Ar g Ser Thr Al a Val 120 Val Ser Ser Phe Thr 200 Thr Gy Leu Phe Val 105 Lys Asp G u Gy Al a 185 G u Asp Lys Hi s Pr o 265 Ser G u Gy Ser 155 Leu Val Leu G n Pr o 235 Al a G u Al a Gy I e 140 Leu SIle Leu Ser Val 220 Ar g Al a Al a l Ie Gy 125 Pr o Gy Al a Su I e 205 Leu Phe G n G u Ar g 110 Val Val Gy Asp bet 190 Thr Val Val Al a Ser 270 G y Thr Val Vet G u lie Val G y His Val 160 Al a Ar g 175 Val Pr o Thr Ile Trp G n Arg G u 240 Tyr Ile 255 Phe <210> 617 <211> 930 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(907) <223> FRXA01929 <400> 617 tgactccata acgagaactt aatcgagcaa cac tatttattct gagctggtca tcacatctat act gca Al a cag G n ttt Phe aac aaa Lys gtt Val gag G u gt g cgc Ar g gtt Val ggc gty ggt cgt Ar g acc Thr gat Asp gat ttc Phe t at Tyr ct c Leu acc ccct gaa cagt gaat ca aat cggaat t catgccc atg tca ggc att gat IVet Ser Gy lie Asp 1 cgc gaa gct aaa gta aac ggc Arg G u Ala Lys Val Asn y 15 gat gcg ctt tcg gcg cgc att Asp Ala Leu Ser Ala Arg lie ctt gtt ggt gat tcc gct gcc Leu Val y Asp Ser Ala Ala ttg tcg atc acc ttg gat gag Page 767 seqi i st car r ect ed. t xt Asn Val Val Leu G31y Ar g Asp Thr Thr Leu Ser I Ie Thr 60 Leu Asp 03 u at g vb t gt g ValI gcg A a gt g ValI gt t ValI t cc Ser 1 50 t ct Ser tt t Ph e ac c Thr ac a Thr ggc G31y 230 tt g Le u tt c Ph e gt g ValI gt t ValI gag O31u at c I Ie 120 gct A a cat Hi s aag Ly s gt t ValI g at As p 200 ggg G31y aag Ly s g ac As p ggc G31y gcc A a ct g Le u gcg A a ggt G31y at t I Ie tt g Le u at c I Ie 1 70 tt g Le u t cc Ser gt t ValI cgc Ar g gcg A a 250 gcg A a tt t Ph e cgg Ar g gt g ValI gt t ValI 140 ggc G31y g at As p at g vb t ac c Thr gt g ValI 220 gt c ValI gcc A a ac g Thr ac c Thr at g vb t 110 at c G31y gt g ValI cgc Ar g cca Pr o 1 90 at c I Ie c ag O31n gag (31u at c I Ie at c I Ie t at Tyr 95 cgt Ar g gcg A a c ac Hi s gt t ValI gcg A a 1 75 gca A a gga G31y g at As p t ac Tyr gcc A a 255 gct A a gag O31u g aa O31u c ag O31n at c I Ie c ag O31n 1 tt g Le u gag (31u at c I Ie gcc A a gcc A a 240 g at As p aag Ly s ag c Ser ggt G31y at t I Ie 130 t ac Tyr cgt Ar g c ag O31n gcg A a gcc A a 210 ggc O31y tt g Le u c ac Hi s cgt Ar g cca Pr o gcg A a 115 cga Ar g ac c Thr ggc O31y gcg A a cgc Ar g 1 ggc O31y ct c Le u ggc O31y gcg A a gcg A a aat As n 1 00 gct A a cgc Ar g ccg Pr o gcg A a ggt O31y 1 gag O31u aat As n aac As n g at As p ggt O31y 260 gcg gag tcc ttt A a 03 u Ser Phe taatgcaggt agcaaccaca aag <210O> 618 <21 1> 269 <21 2> PRT <21 3> Cor ynebact er i urn g1 <400> 618 rvbt Ser O3y I Ie Asp Ala 1 5 Ala Lys Val Asn O31y O31n ut anm cumn Lys Lys lie Arg Thr Arg His Phe Arg Ou 10 Lys Val Ser Val Leu Thr Ser Tyr Asp A a 25 Page 768 Leu Ser Ala Ar g Il e Phe Asp 03 4 seqI i st car r ect ed. t xt u Ala Gy Val Asp I'bt Leu Leu Val 0 Gy le Thr ValI Thr Thr G31y 145 G31y O31u A a G31y Ph e 225 Thr I Ie Ser Le u Ar g Pr o A a 115 Ar g Thr G31y A a Ar g 1 95 G31y Le u G31y A a A a O31u Le u O31n A a I Ie O31n Ser 1 65 A a ValI G31y Ar g Ser 245 Thr ValI li1e ValI ValI Ly s As p 135 O31u G31y A a O31u As p 215 Ly s Hi s Pr o Le u Le u As p Ser 1 O31u G31y Ser Le u ValI 1 Le u O31n Pr o A a (31u 265 G31y A a Le u 90 A a G31y I Ie Le u I Ie 1 70 Le u Ser ValI Ar g A a 250 A a Ar g Ly s 75 Pr o I Ie G31y Pr a G31y 1 55 A a Ou le Le u Ph e 235 O31n (31u As p A a Ph e Ar g ValI ValI 140 O31y As p vb t Thr ValI 220 ValI A a Ser Thr ValI O31y ValI O31u 125 ValI Hi s A a ValI Thr 205 Tr p Ar g Tyr Ph e Le u I Ie Tyr Ar g A a Hi s ValI A a 1 A a O31y As p Tyr A a 255 Ser A a O31u O31u O31n I Ie O31n 1 Le u Ou le A a A a 240 As p <210O> 619 <21 1> 921 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (898) <223> RXA01521 <400> 619 accccggcag gcaacgcctt ttccgggatt tggcgcgcag gcaggcagag atttcccgcg cgcaagat at t gagct gtgg9 gcaat tcaga aggaggaccg t tg agt t tc acg cat Leu Ser Phe Thr Hi s 1 ggt cag ggc aga gt t t tt gat acc gt cgag cag at ccgc at gt t cggc Oy On Oy Arg Val Phe Asp Thr Val Ou On Ilie Ar g N/bt Phe O31y 15 agc gcc ctg cgc aaa acc ggc aaa cca gtg gtg ctc gta ccc ttg gga Page 769 Ser Ala Leu Arg Lys seql i st cor r ect ed. t xt Thr y Lys Pro Val Val Leu Val 30 Pro Leu G y aat Asn at c SIle cac Hi s ccc Pr o cca Pr o ttg Leu gag G u gt a Val 150 at t l Ie ttg Leu ggc G y ggc Gy gcc Al a 230 gat Asp aaa Lys ct c Leu ggt Gy cgt Ar g acg Thr ctt Leu at c SIle 120 gt g Val ct g Leu ct g Leu ct g Leu gca Al a 200 acc Thr gaa G u caa G n at c SIle gca Al a gt g Val agg Ar g tgg Tr p cca Pr o gga Gy ct a Leu tct Ser aat Asn 170 gca Al a gtt Val gat Asp gga Gy cac Hi s 250 ggc G y gt c Val gaa 75 cct Pr o caa G n gca Al a gt c Val gtt Val 155 at t SIle gcc Al a aaa Lys t at Ty r gac Asp 235 gac Asp cat at t His Il e 45 gtc gt c Val Val gag ctt G u Leu cac ggc Hi s G y ggt gcg G y Al a act gat Thr Asp 125 cag cgc G n Arg 140 cca acc Pro Thr t ca gt g Ser Val ctc acc Leu Thr gaa aca G u Thr 205 gta gaa Val G u 220 gcc cga Al a Ar g aac gt c Asn Val gcg Al a gcc Al a at c SIle at c SIle gaa G u 110 gt g Val gcc Al a gt g Val ccc Pr o gcc Al a 190 gt c Val at c SIle ct c Leu ggc Gy ct c Leu t at Tyr gac Asp cgg Ar g 95 gt a Val gt g Val ctt Leu cgc Ar g gaa G u 175 ggt G y acg Thr cgt Ar g ttc Phe ct a at c SIle gcc Al a gcg Al a 80 gt g Val acc Thr ct c Leu aat Asn at g let 160 gac Asp gcg caa G n ggc Gy gca Al a 240 ccc cgc Ar g ggc Gy at c l Ie gaa G u aag Lys ggt Gy gat Asp 145 cca Pr o t cc Ser cat Hi s gt g Val ct g Leu 225 gcc Al a ctt gca Al a ccg Pr o ttc Phe gtt Val gt g Val gaa G u 130 ct g Leu gat Asp cgc Ar g tcg Ser ct c Leu 210 gat Asp at c l Ie gga Gy gca Al a gaa G u ccg Pr o aca Thr ct g Leu 115 aag Lys cat Hi s gga G y gaa G u gca Al a 195 aaa Lys ctt Leu acg Thr at c SIle aaa Lys tcg Ser ttc Phe ggt Gy 100 ggg Gy gac Asp at t SIle ct a Leu acg Thr 180 gaa G u gcc Al a gga Gy ctt Leu ggc G0y 260 cgc Ar g gat Asp aat Asn ggc Gy ctg Leu t at Tyr cca Pr o gcc Al a 165 gca Al a cac Hi s gca Al a cca Pr o ggc Gy 245 ttc Phe Leu Pro Leu gaa ggc 2u G 265 tgatcccggt ttacccagtt cgc <210> 620 <211> 266 <212> PRT <213> Corynebacteri um gl utami cum Page 770 seql i st cor r ect ed. t xt <400> 620 Leu Ile Leu Ar g Gy I le G u Lys Gy Asp 145 Pr o Ser Hi s Val Leu 225 Al a Leu Ser Phe Thr Arg Met Phe Val Pro Leu Al a Al a Lys Pro u Ser Phe Pro Phe Val Thr G y 100 Val Leu G y 115 G u Lys Asp 130 Leu His Il e Asp G y Leu Arg G u Thr 180 Ser Al a G u 195 Leu Lys Al a 210 Asp Leu G y IIe Thr Leu G v lie v Hi s Gy Sy Ar g Asp Asn Sy Leu Tyr Pr o Al a 165 Al a Hi s Al a Pr o Sy 245 Phe Sy n y Arg Ser Asn SIle Hi s 70 Pr o Pr o Leu G u Val 150 l le Leu Sy Sy Al a 230 Asp Lys Al a Sy Pr o Al a G u Thr Sy Leu 135 Lys Ser Ser G u Val 215 Pr o Val Asn Ar g 25 Hi s Al a Leu Leu Thr 105 Thr Val Hi s Ar g Al a 185 Val Pr o SIle Leu G u 265 Val 10 Lys Al a Val Ar g Tr p 90 Pr o Sy Leu Ser Asn 170 Al a Val Asp Sy Hi s 250 Sy Phe Asp Thr Val Thr Sy Val G u 75 Pr o G n Al a Val Val 155 I le Al a Lys Tyr Asp 235 Lys I e Val Leu Sy Al a Asp 125 Ar g Thr Val Thr Thr 205 a u Ar g Pr o Al a Al a SIle SIle G u 110 Val Al a Val Pr o Al a 190 Val SIle Leu G u G n Val Val Leu II e Tyr Al a Asp Al a Arg Val Val Thr Val Leu Leu Asn Arg IVet 160 Su Asp 175 G y Al a Thr G n Arg G y Phe Al a 240 Leu Pro 255 Asp Asn Val y <210> 621 <211> 1137 <212> DNA <213> Corynebacter i um gl utami cum <220> <221> CDS <222> (101)..(1114) <223> RXS01145 <400> 621 taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat ttacagtggg gggcagacat cttttcacca aaatttttac gaaaggcgag attttctccc atg gct att gaa ctg 115 Page 771 seqi i st car r ect ed. t xt [vb t Al 1 a I Ie 3 u Leu ctt tat gat gct Leu Tyr Asp Al a gac Asp gct gac ct C Al a Asp Leu 10 gcc Al a cgc Ar g t cc Ser gag O31u t cc Ser ggc G31y at c I Ie cca Pr o t gc Cys 150 acc Thr cca Pr o gct Al a gt t Val ct t Leu 230 agc Ser gt t Val t ct Ser gag O31u gca Al a gca Al a gca Al a cca Pr o 120 cac Hi s at c l Ie t cc Ser acc Thr ct C Leu 200 acc Thr gag O31u at g lvb t t ac Tyr gt t Val gca Al a t gg Tr p at c l Ie ct g Leu gac Asp gt t Val gt c Val gca Al a 170 gaa O31u ggt G31y gct Al a aag Lys t ac Tyr 250 ggc G31y gag O31u aag Lys gct Al a 75 tt C Phe tt C Phe gac Asp cgc Ar g gac Asp 155 gca Al a gct Al a ggc G31y ggc G31y ct C Leu 235 t ct Ser t cc Ser gt t Val gaa O31u gac Asp acc Thr ggc G31y at c l Ie cgt Ar g 140 cag O31n gca Al a gag (31u acc Thr t ac Tyr 220 at c l Ie gt t Val t cc Ser ggc G31y at t l Ie ggc G31y at c l Ie gac Asp ggc G31y 110 gt t Val ttc Phe cca Pr o ggt G31y gt c Val 190 gaa (31u cca Pr a gac Asp gac Asp at c l Ie gca Al a ct g Leu gag (31u ct C Leu 80 gag (31u aac Asn at g lvb t gat Asp gga G31y 160 gca Al a gac Asp gt c Val at g lvb t at g lvb t 240 gct Al a ggc G31y t cc Ser gag (31u aag Lys gct Al a aac Asn Cac Hi s gcg Al a 130 aag Lys gca Al a gca Al a ttc Phe gt t Val 210 t ac Tyr gaa (31u ttc Ph e cgt Ar g Cag O31n ggc G31y acc Thr cca Pr a ct g Leu ttc Phe 115 cca Pr a ggt G31y Cag O31n ggc G31y ggc G31y 195 ggc G31y ttc Phe ggt gy ggt G31y aag Lys aac Asn t cc Ser acc Thr gac Asp aac Asn 100 gac Asp aag Lys gt t Val gct Al a gt t Val 180 gag O31u ttc Phe gag O31u ggc G31y ggc 31y 260 gt t Val ct C Leu aag Lys gct Al a acc Thr gca Al a ct g Leu ggc G31y cct Pr a ct g Leu 165 at c l Ie Cag O31n gag O31u gt t Val at c l Ie 245 t ac Ty r ctc tcc ggc cca cgc gtc atc gat gca gac acc aag tcc cgc atg aag Page 772 seqI i st car r ect ed. t xt Leu Ser O31y Pro Arg Val lieAsp Ala Asp Thr Lys Ser 265 270 gac atc ctg acc gat atc cag gac ggc acc ttc acc aag Asp Ilie Leu Thr Asp Ilie O31n Asp O31y Thr Phe Thr Lys 280 285 290 gca aac gtt gag aac ggc aac acc gag ctt gag ggc ctt A a Asn Val (1u Asn(1y Asn Thr(1u Leu (1u (1y Leu 295 300 305 ac aac aac cac cca at c gag gag acc ggc gct aag ct C Tyr Asn Asn Hi s Pro Ilie O31u O31u Thr O31y Ala Lys Leu 310 315 320 at g agc t gg gt c aag gt t gac gct cgc gca gaa acc gct I'b t Ser Tr p Val Lys Val Asp Ala Ar g Ala O31u Thr Ala 330 335 ccct t tgacg gct <210> 622 <211> 338 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn Ar g I'bt Lys 275 cgc ct c at c Ar g Leu I Ie cgt gct t cc Ar g Ala Ser cgc gac ct C Arg Asp Leu 325 t aagt t t cac 979 1 027 1 075 1124 1137 <400> 622 I'b t A a I I e 03 u Leu Leu Tyr Asp A a O31n Hi s Ar g ValI Le u Pr a I Ie ValI O31y 145 Thr Ar g Le u Ar g O31n G31y Thr Pr a Le u Ph e 115 Pr a G31y O31n G31y O31y 1 95 ValI Le u Lys5 A a Thr A a Le u O31y Pr a Le u 1 65 li1e O31n A a Ar g Ser O31u 70 Ser O31y I Ie Pr a Cy s 150 Thr Pr a A a I Ie As p A a A a O31n As p Lys5 O31y 135 Le u Le u Thr ValI ValI Ser 40 O31u A a A a A a Pr a 120 Hi s I Ie Ser Thr Le u 200 O31y O31y Lys5 A a O31u Le u 1 A a Le u A a Tyr Ph e 1 Cys As p 10 Tyr ValI A a Tr p I Ie 90 Le u As p ValI ValI A a 1 70 O31u O31y A a O31y O31u Lys5 A a 75 Ph e Ph e As p Ar g As p 1 55 A a A a O31y As p Ser ValI O31u As p Thr O31y I Ie Ar g 140 O31n A a O31u Thr Le u O31n ValI A a ValI As n Hi s I Ie 125 O31n As p I Ie Thr O31u 205 Le u Hi s O31y Ph e vb t I Ie Le u O31y ValI Thr O31y 1 Thr Le u I Ie A a Le u O31u Le u O31u As n vb t As p O31y 1 A a As p ValI Lys Val O31y Phe O31u Val Leu Thr 03 u A a 03 y Tyr Ou Pro Ou I'bt Page 773 Al a 225 Ph e (31u Ly s Thr G31y 305 Ly s Thr Ph e G31y G31y Ar g 275 Ar g Ar g Ar g ValI I Ie 245 Tyr Ly s I Ie Ser Le u 325 Le u 230 Ser Le u As p Al a Tyr 310 vb t 215 Hi s As n Ser I Ie As n 295 As n Ser seql i st car r ect ed. t xt 220 G3 u Leu Lys Leu I I e Val 235 N/t As n Ty r Ser Val Ser 250 G31y Pr o Ar g Val I Ie As p 265 Leu Thr As p I I e G3 n As p 280 285 Val O31u As n G31y As n Thr 300 As n Hi s Pr o I Ie (31u (31u 315 Tr p Val Lys Val As p Al a 330 As p As p Al a 270 G31y O31u Th r Ar g Le u Th r 255 As p Th r Le u G31y Al a 335 <210O> 623 <21 1> 556 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (556) <223> FRXA01145 <400> 623 t aat gt agt t gt ct gcccaa gcgagt t aaa ct cccacgat ctt t tcacca aaat tt t tac gaaaggcgag att t tct ccc ct t Le u gcc Al a cgc Ar g t cc Ser gag (31u t cc Ser g at As p gt t ValI t ct Ser gag (31u gca Al a gca Al a g ac As p t ac Tyr gt t ValI gca Al a t gg Tr p at c I Ie gct Al a ggC G31y gag (31u aag Ly s gct Al a 75 t t c Ph e g ac As p t cc Ser gt t ValI g aa (31u g ac As p ac c Thr ct c Le u c ag O31n gt c ValI 45 gca Al a gt c ValI aac As n t cc Ser ggC G31y at t G31y at c I Ie g ac As p t tg at c Leu I I e 15 cac gca Hi s Al a ggt ct g 03 y Leu ttc gag Phe 03 u at g ct c l'b t Leu 80 at c gag I Ie G31u 95 Page 77z t tacagt ggg gggcagacat at g gct at t gaa ct g l'b t Al a I I e 03 u Leu 1 cag ggc cgt aag gtt O31n G31y Ar g Lys Val cac t cc cag aac ct C Hi s Ser 03 n Asn Leu cgc gag ggc t cc aag Ar g O31u G31y Ser Lys gt c aag acc acc gct Val Lys Thr Thr Al a ct g gct cca gac acc Leu Ala Pro Asp Thr cca aac ctg aac gca Pro Asn Leu Asn Ala 1 00 seql i st cor r ect ed. t xt ggc gac gca ctg ctg ttc ggc cac ggc ctg aac att cac ttc gac ctg 451 G y Asp Al a Leu Leu Phe G y Hi s G y Leu Asn lie Hi s Phe Asp Leu 105 110 115 atc aag cca gct gac gac atc atc gtt ggc atg gtt gcg cca aag ggc 499 Ile Lys Pro Ala Asp Asp Ile Ile Val Gy ebt Val Ala Pro Lys y 120 125 130 cca ggc cac ttg gtt cgc cgt cag ttc gtt gat ggc aag ggt gtt cct 547 Pro G y His Leu Val Arg Arg G n Phe Val Asp G y Lys G y Val Pro 135 140 145 tgc ctc atc 556 Cys Leu Il e 150 <210> 624 <211> 152 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 624 IVet Ala lie G u Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 1 5 10 Sn G y Arg Lys Val Ala I I e Val G y Tyr G y Ser n G y His Ala 25 His Ser G n Asn Leu Arg Asp Ser G y Val G u Val Val I I e G y Leu 40 Arg G u y Ser Lys Ser Ala G u Lys Ala Lys G u Ala y Phe G u 55 Val Lys Thr Thr Ala G u Ala Ala Ala Trp Ala Asp Val lie IVet Leu 70 75 Leu Ala Pro Asp Thr Ser n Ala u lie Phe Thr Asn Asp Ile u 90 Pro Asn Leu Asn Ala G y Asp Ala Leu Leu Phe G y His G y Leu Asn 100 105 110 lie His Phe Asp Leu IIe Lys Pro Ala Asp Asp IIe IIe Val G y IVet 115 120 125 Val Ala Pro Lys G y Pro G y His Leu Val Arg Arg G n Phe Val Asp 130 135 140 Sy Lys y Val Pro Cys Leu Ile 145 150 <210> 625 <211> 1389 <212> DNA <213> Corynebact er i um gl ut am cum <220> <221> CDS <222> (101)..(1366) <223> RXA02239 <400> 625 ctatgcacag cggaggtcga gggagagcgt ggggtggcgt cgaaaagcat cttgtagagt gtgtgggaac ccagccagcc tcttactttg aaggattgtt gtg ccc atg act cat 115 Page 775 seql i st cor r ect ed. t xt Val Pr o 1 lMt Thr His gtt tca agc ccc tcc gca ccc cgc Val Ser Ser Pro Ser Ala Pro Arg gga Gy gcg Al a gt g Val acg Thr gaa G u cgt Ar g gt g Val tgg Tr p ggg G y 150 aca Thr aat Asn aaa Lys cgc Ar g gaa G u 230 gct Al a gca Al a gat Asp aag Lys ttt Phe gat Asp gt g Val acg Thr 120 aat Asn acc Thr cct Pr o gt g Val ct g Leu 200 at t SIle gca Al a gag G u t ac Tyr gt g Val acg Thr gcg Al a at c SIle ggt Sy ccc Pr o gct Al a at t SIle cgg Ar g 170 gcc Al a act Thr aat Asn cag G n ccc Pr o 250 aag Lys cgg Ar g ttt Phe gt g Val 75 gt g Val ct c Leu gt g Val acc Thr gag G u 155 ct g Leu ggg Gy gct Al a agt Ser cga Ar g 235 act Thr gcg Al a gt g Val gaa G u gat Asp at t SIle ggt G y gtt Val gt a Val 140 cct Pr o ccg Pr o gcg Al a ggt Gy t cc Ser 220 ggt Gy ccg Pr o tgt Cys gtt Val 45 gcg Al a tcg Ser gcg Al a gac Asp at t SIle 125 gcc Al a gcg Al a gat Asp agg Ar g ggc Gy 205 tcg Ser gct Al a gca Al a aac Asn cac Hi s cct Pr o ttg Leu gt g Val ccg Pr o gat Asp 110 gcg Al a aat Asn cat Hi s cca Pr o ttg Leu 190 acg Thr ggc Gy cat Hi s ggc Gy gt g Val 15 at c SIle acg Thr tct Ser cag G n gcg Al a 95 ct g Leu ccg Pr o gt g Val ggt G y gag G u 175 cct Pr o cat Hi s cgt Ar g gt c Val gca Al a 255 gtt Val gt g Val gaa G u ggc Gy cat Hi s 80 aca Thr ttg Leu gcc Al a gca Al a cga Ar g 160 cag G n cag G n gag G u caa G n agc Ser 240 gag G u gtt Val cgc Ar g t cc Ser aat Asn gt g Val gcc Al a gcg Al a at g Met acg Thr 145 ct c Leu at t SIle gat Asp cat Hi s ggt Gy 225 at c SIle at c SIle ggt G y gcg Al a gcg Al a ccg Pr o aaa Lys gat Asp gcg Al a cat Hi s 130 ct g Leu acc Thr gtt Val ttg Leu at t SIle 210 ttt Phe gt g Val gt g Val gtt Val ttt Phe ttg Leu gt g Val gtt Val ttg Leu acg Thr 115 acg Thr agg Ar g ggt G y gat Asp gcg Al a 195 gat Asp gcg Al a gcg Al a ccg Pr o gcc Al a aaa Lys aag Lys tct Ser ggc Gy at g Met 100 ct g Leu gag G u cag G n aaa Lys tta Leu 180 ggc G y cct Pr o ttg Leu gga G y gt g Val 260 ggc G y gaa G u ttt Phe aca Thr cag G n gcg Al a ctg Leu at g let cgg Ar g gat Asp 165 gcc Al a aag Lys gtg Val ggt G y aat Asn 245 gtg Val tcc aca caa gac atg ttt gat gca gtc cag gaa cga gct ggc caa tct Page 776 Ser Thr G3 n [Vbt Phe Asp Al a eqI i st car r ect ed. t xt Val On Ou Arg Al a G3 y G3 n Se r 275 g at As p c ag O31n tt g Le u 310 gt g ValI ggt G31y gcg Al a gag O31u at t I Ie 390 at c I Ie gt c ValI at c I Ie 280 ac a Thr ac c Thr cgt Ar g gct Al a aag Ly s 360 ggc G31y g at As p gt t ValI at g vb t aag Ly s t cg Ser g aa O31u 330 g aa O31u ct g Le u ggc G31y c ac Hi s gcg Al a 410 gcg Al a t t g Le u t t g Le u 315 gca Al a act Thr cag O31n aaa Ly s ggt G31y 395 caa O31n gca Al a aag Ly s 300 gt g ValI gga O31y gga O31y aag Ly s gt g ValI 380 ggg O31y at t I Ie gcg Al a 285 aag Ly s g aa O31u gag O31u g ac As p aag Ly s 365 tt t Ph e gt a ValI t gg Tr p 270 gt a ValI O31y aac As n ct g Le u gag O31u 350 99c O31y O31y gt c ValI g ac As p gct Al a t ca Ser ccg Pr o g ac As p 335 c ac Hi s t gc cys c aa O31n g at As p gcg Al a 415 g at As p g at As p g at As p 320 agt Ser ac c Thr g ac As p aag Ly s gt g ValI 400 gca Al a ac g Thr 290 g at As p tt g Le u cct Pr o gcc Al a ct c Le u 370 aat As n c ac Hi s gcg Al a ccc Pr o g aa O31u gct Al a gt c ValI tt g Le u 355 at g vb t gag O31u O31y t at Tyr gca Al a g ac As p ac c Thr at c I Ie 340 gag O31u t gt cys ggc O31y ag c Ser cgc Ar g 420 t cg Ser gca Al a ac g Thr 325 gt g ValI t at Tyr aat As n t gg Tr p aaa Ly s 405 g aa O31u 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1389 t agaaaaat c cagct agacc act <210O> 626 <21 1> 422 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 626 Val Pro l'bt Thr His Val Ser Ser Pro 1 5 Val 31y ValI Ala Oy Oy Ile AlaAla Ar g Al a Phe Lys O31u Al a O31y Asp As n 40 Ser Al a Leu Lys Phe Val O31y Lys Al a 55 As n Pr o Val Ser Thr Thr Val Phe Asp 70 Val Lys Val O31y O31n O31u Ala Asp Leu Se r Al a Pro 10 Ty r Lys Al a Val Ar g Val Thr Phe 03 u Al a Val As p li1e Val I Ie 90 Page 777 Ar g cys ValI Al a Ser Al a ValI lie Th r Se r O31n Al a Ala Asp Leu IVet 100 Al a Ar g Val seql i st cor r ect ed. t xt Val Ala Gy Leu Gy Asp 105 Asp Leu Leu 110 Al a et Thr 145 Leu SIle Asp Hi s Gy 225 I e I e Ar g Phe Ser 305 I le Asn Thr Leu Hi s 385 G u Leu Thr 115 Thr Ar g Sy Asp Al a 195 Asp Al a Al a Pr o Sy 275 Pr o G u Al a Val Leu 355 bet G u Gy Ty r Leu Leu Val G u Met Tr p G n Arg G y 150 Lys Asp Thr 165 Leu Al a Asn 180 G y Lys Lys Pro Val Ar g Leu G y G u 230 G y Asn Al a 245 Val Val Ser 260 G n Ser Asp Al a Ser G n Asp Al a Leu 310 Thr Thr Val 325 Ile Val y 340 G u Tyr Al a Cys Asn u Sy Trp lle 390 Ser Lys Ile 405 Arg u Val 420 Al a Phe 135 I le Sy Al a Val Phe 215 I le Al a Thr Phe Al a 295 Ser Lys Phe Ar g Val 375 Leu Thr 120 Asn Thr Pr o Val Leu 200 I le Al a G u G n SIle 280 Thr Thr Ar g Al a Lys 360 Sy Asp Cys Pr o Val Sy Hi s 185 I le Sy Al a Leu Asp 265 Val Ser SIle Ar g Al a 345 Lys bet Al a Pr o Al a SIle Ar g 170 Al a Thr Asn G n Pr o 250 Met bet Lys Ser G u 330 G u Leu Sy Hi s Al a 410 Val Thr G u 155 Leu Sy Al a Ser Ar g 235 Thr Phe Al a Leu Leu 315 Al a Thr G n Lys Sy 395 SIle 125 Al a Al a Asp Ar g Sy 205 Ser Al a Al a Al a Al a 285 Lys G u G u Asp Lys 365 Phe Val Al a Asn Hi s Pr o Leu 190 Thr Sy Hi s Sy Val 270 Val Sy Asn Leu G u 350 Sy Sy Val Pr o Val Sy G u 175 Pr o Hi s Ar g Val Al a 255 G n Al a Ser Pr o Asp 335 Hi s Cys G n Asp Su Val Ala Sn lie Trp Asp Al a Al a 415 <210> 627 <211> 1092 <212> DNA <213> Corynebacter i um gl ut ai cum Page 778 seqi i st car r ect ed. t xt <220> <221 OIDS <222> (1069) <223> RXA00581 <400> 627 gcat gagt tt act cacgt gc ccacgt ct tt t agccaccca cgat cacact agt ggagt ag ct aaggt gca caat ggat t c t tgaagt gaa aaaat aaccc atg gca gag caa aac [vbt A a G3 u G3 n As n gct A a ag c Ser t ca Ser at c I Ie ct g Le u gca A a tt t Ph e gcc A a gt g ValI at c I Ie 1 50 c aa O31n g aa O31u O31y tt a Le u ag c Ser t ac Tyr cct Pr o g aa O31u cgt Ar g ac c Thr at t I Ie ct c Le u 120 ct c Le u cgc Ar g gca A a aac As n tt c Ph e 200 gt c ValI act Thr g at As p gt g ValI at t I Ie at t I Ie ac c Thr gt c ValI c aa O31n ac c Thr tt a Le u ct c Le u 1 70 cct Pr o ac a Thr c aa (31n gt g ValI g ac As p ac c Thr ct c Le u ct c Le u ct c Le u gga O31y ct g Le u g ac As p 140 t cc Ser tt t Ph e t ac Tyr cgc Ar g ggc (31y 220 aaa Ly s cgc Ar g c aa O31n 45 gct A a c ag O31n gga O31y t cc Ser ct t Le u 125 gga O31y cga Ar g gt c ValI t cc Ser c aa O31n 205 cca Pr o cct Pr o gca A a aaa Ly s g aa O31u gt a ValI act Thr gt c ValI 110 c ag O31n tt c Ph e aaa Ly s ac c Thr c ac Hi s 1 90 ccc Pr a ac a Thr t cc Ser 15 c aa O31n g aa O31u gt g ValI gcg A a t cc Ser 95 gcc A a cgc Ar g ct c Le u gga O31y g ac As p 1 75 ac c Thr g ac As p cca Pr a t gg Tr p gt c ValI gca A a gcc A a 80 ccc Pr a gt c ValI t gg Tr p t at Tyr tt c Ph e 1 60 gt a ValI gcg A a at t I Ie 1 cgc Ar g cgc Ar g at t I Ie g aa O31u 65 cga Ar g t ct Ser ggt O31y aat As n ccc Pr a 145 ccc Pr a aaa Ly s t ac Tyr tt g Le u ac a Thr gag O31u g aa O31u 50 gt c ValI c ag O31n at c I Ie aaa Ly s t cc Ser 130 ggc O31y g aa O31u t cc Ser g ac As p at c I Ie 210 cca Pr a ct g Le u 35 ct t Le u t ac Tyr c aa O31n t ct Ser t ca Ser 115 c ac Hi s gcg A a ag c Ser gga O31y cga Ar g 1 gt c ValI g at As p 20 ag a Ar g cga Ar g ct t Le u ct t Le u gt g ValI 1 00 ac c Thr ccc Pr a g aa O31u t ac Tyr aaa Ly s 1 gt t ValI g aa O31u tt c Ph e aac As n ggc O31y ccg Pr a act Thr ccg Pr a ac c Thr cgc Ar g ct a Le u g ac As p 1 ct c Le u cca Pr a O31y t tg at g gt c agt gac ct t tt c Leu N/t Val Ser Asp Leu Phe 225 Page 779 gac t tc agc gt c t ac gt a g~ As p Phe Ser Val Ty r Val 230 235 t ac at c gac cgc t tc ct c a~ Ty r I Ie As p Ar g Phe Leu L~ 250 ggt gcc cac t tc t cc cat t~ G31y Al a Hi s Phe Ser Hi s T~ 265 gcc gt c gct cga gaa ct g t~ Al a Val Al a Ar g O31u Leu Tr 280 gag aat at t ct t ccc acc c~ O31u As n I Ie Leu Pr o Thr Ar 2953 ggt agc gat cac t tg gt g g~ G31y Ser As p Hi s Leu Val 03 310 315 t aggggt tct t gctggttt t gag <210> 628 <211> 323 <21 2> PRT <21 3> Cor ynebact er i urn gl ut 3p ts g U seqI i st car r ect ed. t xt gcc cgc acc gaa gat at c Al a Ar g Thr O31u Asp I Ie 240 ct c cgc gac act gca tt c Leu Arg Asp Thr Ala Phe 255 gcc gac at g gct gat cca Al a Asp I'b t Al a Asp Pr o 270 caa t cg at c aac ct g ccc O31n Ser Ilie Asn Leu Pr o 285 290 gt t cgc gcg t cg t tg gt a Val Ar g Al a Ser Leu Val 305 cgg gt g agg at g cgc aag Ar g Val Ar g I'b t Ar g Lys 320 g aa O31u cgt Ar g gag O31u 275 aac As n ct g Le u at c I Ie aaa cgc Ar g 260 t cc Ser tt g Le u aaa 835 883 931 979 1 027 1 069 1 092 ani cumn <400> 628 [vb t Al a 03 u 03 n As n Al a Ar1 Ar g O31u Ar g Ser G31y As n Pr a 145 Pr a Pr a Le u Le u Tyr O31n Ser Ser 115 Hi s Al a Ser Ph e As n G31y Pr a Thr Pr a Thr Ar g Le u As p 1 65 Ser Ser I Ie Le u 70 Al a Ph e Al a ValI I e 150 O31n Al a Se r Thr Pr o Tyr Leu 25 I'b t Pr o O31n 40 O31y 1u As n Ser Ar g Leu Al a Thr 03 u Val I Ie O31y 1 05 Arg Leu Leu 120 Asp Leu Val 135 Ar g Ar g O31y Ar g Al a Leu O31y Ph e Le u As p Hi s Ph e Al a ValI Thr vb t 1 Ar g Lys5 Ar g O31n Al a O31n O31y Ser Le u 125 O31y Ar g ValI Pr a Al a Lys5 O31u ValI Thr ValI 110 O31n Ph e Lys5 Thr Ser O31n O31u ValI Al a Ser Al a Ar g Le u O31y As p 1 Lys Ser O31y Lys Leu O31u Val Asn Al a Pro Val Tyr Ser Hi sThr Al a Page 780 Tyr Leu Val 225 Asp Al a Asp Leu Leu 305 Ar g Ar g 195 Val Asp G u Ar g G u 275 Asn Leu SIe Pr o Gy Phe Tr p 245 Pr o SIle Val Lys Gy Leu Asp 230 Tyr Gy Al a G u Gy 310 G u Asn 215 Phe SIle Al a Val Asn 295 Ser seql i st cor r ect ed. t xt 185 Phe Thr Thr Val Arg G n 200 205 Val Leu G n Thr G y Pro 220 Ser Val Tyr Val Asp Al a 235 Asp Arg Phe Leu Lys Leu 250 His Phe Ser His Tyr Ala 265 Ala Arg G u Leu Trp G n 280 285 Ile Leu Pro Thr Arg Val 300 Asp His Leu Val G u Arg 315 Asp Leu Thr Asp 255 bet SIle Al a Ar g SIe NMt G u 240 Thr Al a Asn Ser bet 320 <210> 629 <211> 1023 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1000) <223> RXS00838 <400> 629 tcgtctaata gtgctgccaa tccaccggcc at t gatgact tgcttacaaa tcttatctgt gctcaggcaa gatagcaggt gtt Val tct Ser ttg Leu gt a Val gt a Val gaa gct Al a gca Al a tct Ser at t SIle at g Met ttg gca Al a at c l Ie gga Gy gca Al a gct Al a ggg ggt Gy at g Met cac Hi s gcg Al a aaa Lys cct gga Gy gtt Val at c SIle 45 agc Ser gca Al a gcg t at Tyr gca Al a aac Asn gt g Val tta Leu aat ttc gga Phe G y 15 cgt gga Arg G y gat gca Asp Al a caa gaa G n u tcg cgg Ser Arg 80 tcg gtg Page 781 cct t t gtaga gaaggggt ag atg aaa att gcg atc IVet Lys Ile Ala lie 1 gcg ttg tta caa gaa Ala Leu Leu G n G u cga aca tta gaa gcc Arg Thr Leu G u Ala aga ggc gaa cgc tac Arg Gy G u Arg Tyr cta aaa gat gca gat Leu Lys Asp Ala Asp tct tta gat ctc gct Ser Leu Asp Leu Ala gtc gcg att act cag (3 u Leu Leu G3 y G31y seqI i st car r ect ed. t xt Ilie Pro Ala Asn Ser Val Val Ala 95 I I e Th r G3 n 1 00 aat As n cgt Ar g gcc Al a t ct Ser 1 50 at t I Ie gcc Al a c aa O31n at g vb t ag c Ser 230 aat As n gag O31u ggt G31y aaa Ly s at t gaa t ct gct I I e 03 u Ser Al a 1 05 t gg cct ggt gt g Tr p Pr o G31y Val 120 gt g t ca t ac aag Val Ser Ty r Lys gaa ct t t ct agg (31u Leu Ser Ar g 155 gga gt t ct g cat G31y Val Leu Hi s 1 70 t tc gt a gag gt t Phe Val 03 u Val 1 85 ggt acc t tg cgt G31y Thr Leu Ar g 200 gag gt g gct gag (31u Val Al a (31u gcg gtg gag cgc Al a Val (31u Ar g 235 acg agt t cg at g Thr Ser Ser I'b t 250 gag gct cag aca 03 u Al a 03 n Thr 265 aaa act ccg ct t Lys Thr Pro Leu 280 gag gaa aat t ca 03 u 03 u Asn Ser g at As p gt t ValI gga G31y 140 c aa O31n ccc Pr o tt c Ph e ac g Thr gt g ValI 220 ac c Thr c ag O31n ggt G31y cat Hi s ct a Le u cgt Ar g 125 ggc G31y Ph e g at As p ggc G31y cat Hi s 205 gct Al a at g vb t cgt Ar g gca Al a g ac As p 285 gca Al a 110 ggg G31y cca Pr o gca Al a at t I Ie ggg G31y 1 tt t Ph e cgc Ar g aat As n g at As p at t I Ie 270 ct t Le u gcg Al a tt c Ph e ct g Le u ag c Ser tt g Le u 1 75 tt g Le u ag g Ar g gcg Al a tt t Ph e tt g Le u 255 gt g ValI at t I Ie aag Lys5 tt t Ph e t cc Ser act Thr 1 60 gt g ValI ggg G31y gct Al a gca Al a gcg Al a 240 gcc Al a cgg Ar g t at Tyr agt Ser gt t ValI t ac Tyr 145 ct t Le u g at As p gct Al a t cc Ser ggt G31y 225 g at As p gcg Al a gca Al a gct Al a at c I Ie cat Hi s 130 ac g Thr g aa (31u gt g ValI tt c Ph e ct g Le u 210 gt t ValI cgg Ar g gga G31y gcg Al a ggt G31y 290 gct Al a ggg G31y ggt G31y gcc Al a gag (31u 1 g aa (31u gcc Al a tt g Le u cct Pr o gct Al a 260 aaa Lys5 aag Lys5 g at As p cca Pr o g at As p ggt G31y 1 aaa aag tt g Le u ccg Pr a gag (31u 245 agt Ser gt g ValI ct g Le u ct t t agggat aga at caagat cc at g 1 023 <210O> 630 <21 1> 300 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 630 I'bt Lys IleAla IleVal Gy Ala(3y AlaVal GyOy Tyr Phe Oy 1 5 10 Al a Leu Leu O31n 031u Ser Gy Ala Asp Ilie Thr I'bt Val Ala Arg Gy 25 Page 782 Ar g Ar g Leu Ser Val Ser Val Tyr 145 Leu Asp Al a Ser Gy 225 Asp Al a Al a Al a s Thr Leu G u Ala Leu Lys Ser 40 eql i st cor r ect ed. t xt Lys G y Leu His II 4 e Asn Asp Ala G u Asp Asp SIle Gy 115 G u Phe G n Tr p Val 195 G u Al a Met Val Hi s 275 Leu Tyr Asp Al a G n Asp Pr o Asp Gy 165 Lys Lys Leu Pr o G u 245 Ser Val Leu Pr o Val Leu Ser Val Ser 135 Gy Asp Met Leu G u 215 Asp Ser Leu Val G u 295 SIle let Leu SIle Tr p 120 Val G u Gy Phe Gy 200 G u Al a Thr G u Lys 280 G u Pr o SI e Gy G u 105 Pr o Ser Leu Val Val 185 Thr Val Val Ser Al a 265 Thr G u Al a Al a Gy 90 Ser Gy Tyr Ser Leu 170 G u Leu Al a G u Ser 250 G n Pr o Asn Val Thr 75 I le Al a Val Lys Ar g 155 Hi s Val Ar g G u Ar g 235 Met Thr Leu Ser Ser Al a Al a Leu Ar g 125 Gy Phe Asp Gy Hi s 205 Al a lbet Ar g Al a Asp 285 G n Ser Ser Al a Phe Leu Ser Leu 175 Leu Ar g Al a Phe Leu 255 Val SIle G u Ar g Val Lys Phe Ser Thr 160 Val Gy Al a Al a Al a 240 Al a Ar g Tyr <210> 631 <211> 408 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(385) <223> RXC02238 <400> 631 ggcgcttagc caaaacatag agcggtaggg tatgcttatc cgattgagca acctttcccg ctcttaacac tactgtccat atacttttga aaaggtgtca gtg acc aac gtg agc 115 Val Thr Asn Val Ser 1 Page 783 seql i aac gag acc aac gcc acc aag gcc gtc Asn G u Thr Asn Ala Thr Lys Ala Val acc gct cct ccg atc gat gaa ctg ct g Thr Ala Pro Pro lie Asp G u Leu Leu 30 gcc ctc gtg atc ttc gca gcc aag cgt Ala Leu Val lie Phe Ala Ala Lys Arg 45 tac cat cag gca gat gag gga gta ttc Tyr His G n Ala Asp G u G y Val Phe 60 act ccg cag cca ggc gaa aag cca ctt Thr Pro n Pro Gy G u Lys Pro Leu 75 aat gca ggt ctg ttg gac cac gag gaa Asn Al a G y Leu Leu Asp His G u G u cac <210> 632 <211> <212> PRT <213> Corynebacter i um gl ut ai cum <400> 632 Val Thr Asn Val Ser Asn G u Thr Asn 1 5 Pro Pro Val Oy lie Thr Ala Pro Pro Val Thr Ser Lys Tyr Al a Leu Val lle 40 Sn lie Asn Ser Phe Tyr His n Ala 55 lie y Pro Leu Val Thr Pro n Pro 70 Ala Leu Arg u lie Asn Ala y Leu st cor r ect ed. t xt ttc gat ccg cca gtg ggc att Phe Asp Pro Pro Val Gy lie 15 gat aag gtc act tcc aag tac Asp Lys Val Thr Ser Lys Tyr gcg cgc cag atc aac agc ttc Ala Arg n lie Asn Ser Phe gag ttc atc gga cca ttg gtt Su Phe Ile y Pro Leu Val tct att gct ctg cgt gag atc Ser lie Ala Leu Arg u lie 80 ggt taaaagacct tataacttca Sy Al a 10 SIle Phe Asp Sy Leu 90 Thr Asp Al a G u G u Asp Lys G u Al a Sy Lys Hi s Al a Leu Lys Val Pr o G u Phe Asp Al a G u Ser Sy <210> 633 <211> 606 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(583) <223> RXN03058 <400> 633 acgagcttcc gctctgcaca agccgctaga agccccgcat agccctaatg tagagctcat gcccatttgg aatcacaaca ccgcatatcg gccatggctg gtg tca aag ctc aaa 115 Val Ser Lys Leu Lys Page 784 seql i st cor r ect ed. t xt ggc tca aga tcg Sy Ser Arg Ser gac ctg gcc ggc Asp Leu Al a G y att aat aca acc Ile Asn Thr Thr atc acg ttc atc Ile Thr Phe Ile aac aaa gca gtc Asn Lys Ala Val ggc ctc gcc aaa G y Leu Al a Lys caa gct ttt ctc G n Ala Phe Leu 105 atc tac ccc ttc lle Tyr Pro Phe 120 cga caa ctc acc Arg G n Leu Thr 135 atc cac ttc cga Ile His Phe Arg 150 t at aaaccaa aaa <210> 634 <211> 161 <212> PRT <213> Corynebact <400> 634 Val Ser Lys Leu 1 Asp His Ser Phe Val Val Asp Pro G n Ser Tyr Asp Ala G u u G y Lys Leu Leu Ile ctt Leu cgc Ar g ttt Phe gcg Al a cga Ar g aac Asn t cc Ser cct Pr o aag Lys ct c Leu cag G n gaa G u t cc Ser at c I e 75 aaa Lys cga Ar g acc Thr cag G n gac Asp gt c Val gaa G u ct c Leu 60 ct c Leu acc Thr cca Pr o aaa Lys ct c Leu 140 gt c Val gcg Al a ttc Phe 45 cat Hi s aat Asn gcc Al a at c SIle gaa G u 125 ct c Leu ggc Gy cat Hi s 30 cag G n cac Hi s cct Pr o t cc Ser agc Ser 110 ccc Pr o cct Pr o t cc Ser 15 gt c Val ccg Pr o at g Met ggc Gy gac Asp 95 ct c Leu t ca Ser cac Hi s ggc Gy gat Asp acc Thr aac Asn ggc Gy 80 tgg Tr p at c SIle gag G u cgc Ar g 1 gat Asp gt c Val caa G n gcg Al a aag Lys at c SIle aat Asn agt Ser cgt Ar g 145 cac Hi s gt g Val agc Ser gaa G u ct c Leu at c SIle agg Ar g ct c Leu 130 at t SIle tcc ttc gcc Ser Phe Al a gat cct ctt Asp Pro Leu t ac gat gcc Tyr Asp Al a gaa gga ctt G u G y Leu ct c at c gt a Leu Ile Val t cc gga ct a Ser G y Leu 100 gaa caa caa Su Gn n 115 cac gaa ata His G u Ile cgc cgt gga Arg Arg G y tac ctc ctc gag tgg aca aag cct Tyr Leu Leu G u Trp Thr Lys Pro 155 160 taaacagccc er i um gl ut ari cum Lys G y Ser Arg Ser 5 Ala Asp Leu Ala y Leu I e Asn Thr Thr 40 Ala lie Thr Phe Ile Leu Asn Lys Ala Val 70 Val G y Leu Ala Lys Leu Leu 10 Arg G n Phe Gu Al a Ser I Arg Ile I Asn Lys- 90 Page 785 Val Al a Phe Hi s Asn Al a Ser Val Pr o Met Sy Asp seql i st cor r ect ed. t xt lie lie Ser y Leu On Ala Phe Leu Ser Arg Pro lie Ser Leu Ile 100 105 110 Asn Arg G u n G n I e Tyr Pro Phe Pro Thr Lys G u Pro Ser G u 115 120 125 Ser Leu His G u lie Arg n Leu Thr Lys G n Leu Leu Pro His Arg 130 135 140 Arg lie Arg Arg G y I e His Phe Arg Tyr Leu Leu G u Trp Thr Lys 145 150 155 160 Pro <210> 635 <211> 606 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(583) <223> FRXA02903 <400> 635 acgagcttcc gctctgcaca agccgctaga agccccgcat gcccatttgg aatcacaaca ccgcatatcg gccatggctg agccctaatg tagagctcat ggc Sy gac Asp at t SIle at c SIle aac Asn ggc Sy caa G n at c l Ie cga Ar g aga Ar g gcc Al a aca Thr ttc Phe gca Al a gcc Al a ttt Phe ccc Pr o 120 ct c Leu ctt Leu cgc Ar g ttt Phe gcg Al a cga Ar g aac Asn t cc Ser cct Pr o aag Lys ct c Leu cag G n gaa G u t cc Ser at c I e 75 aaa Lys cga Ar g acc Thr cag G n gac Asp gt c Val gaa G u ct c Leu ct c Leu acc Thr cca Pr o aaa Lys ct c gt c Val gcg Al a ttc Phe 45 cat Hi s aat Asn gcc Al a at c SIle gaa G u 125 ct c ggc G y cat Hi s cag G n cac Hi s cct Pr o t cc Ser agc Ser 110 ccc Pr o cct t cc Ser 15 gt c Val ccg Pr o at g Met ggc Sy gac Asp 95 ct c Leu t ca Ser ggc G y gat Asp acc Thr aac Asn ggc Sy 80 tgg Tr p at c SIle gag G u gt g Val 1 gat Asp gt c Val caa G n gcg Al a aag Lys at c SIle aat Asn agt Ser tca aag ctc aaa Ser Lys Leu Lys cac tcc ttc gcc His Ser Phe Ala gtg gat cct ctt Val Asp Pro Leu agc tac gat gcc Ser Tyr Asp Ala gaa gaa gga ctt G u G u G y Leu ct c ct c at c gt a Leu Leu Ile Val atc tcc gga cta I e Ser G y Leu 100 agg gaa caa caa Arg u n n 115 ctc cac gaa ata Leu His G u Ile 130 Leu Leu Pro cac cgc cgt att cgc cgt gga His Arg Arg lie Arg Arg y Page 786 seqI i st car r ect ed. t xt 135 140 145 atc cac t tc cga t ac ct c ct c gag tgg aca aag cct taaacagccc I Ie Hi s Phe Arg Tyr Leu Leu Gu Trp Thr Lys Pr o 150 155 160 t at aaaccaa aaa <210> 636 <211> 161 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 636 Val Ser Lys As p Hi s Ser Val Val As p G3 n Ser Ty r A a G3 u G3 u Lys Leu Leu Il e Il e Ser Asn Ar g G31u 115 Ser Leu Hi s 130 Ar g Ilie Ar g 145 Pr o Leu Lys Phe A a Pro Leu As p A a G3 y Leu I e Val a3 y Leu 1 00 a31na(1n a31u I Ie Ar g a31y a31y Se r Ar g Se r Le u As n Thr Lys5 Le u A a Tyr a31n 135 Hi s A a Thr 40 Ph e A a A a Ph e Pr o 120 Le u Ph e a31y Thr I Ie ValI Lys5 Le u 1 Ph e Thr Ar g Le u 10 Ar g Ph e A a Ar g As n 90 Ser Pr o Lys5 Tyr Leu Asp Val a31y a31n a31u Ser lIe Lys5 Ar g Thr a31n Le u 1 A a Ph e Hi s As n A a I Ie a31u 125 Le u a31u Ser ValI Pr o vb t a31y As p Le u Ser Hi s Thr <210O> 637 <21 1> 783 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (760) <223> RXA00166 <400> 637 ggcgt t tagc gat ct tcaac at cgagcaac cagcgccagc gctt t taccc aaggcagcac gact t at cac gat gt ccgac ct ggat at cc ggct gaggcc gt g gag t t a gcc cgt 115 Val a31u Leu Ala Ar g 1 ggg t tt ggc cga gt c ct g gat gt c ggt gca ggt acc gga aaa cta acc 163 ay Phe ay Arg Val Leu Asp Val ay Ala a1y Thr a31y Lys Leu Thr Page 787 seql i st cor r ect ed. t xt agt gag cta aca gct gat Ser G u Leu Thr Ala Asp atg ttg cgg gtg ttt cgc IVet Leu Arg Val Phe Ar g gcg aca gca gaa cac aca Ala Thr Ala G u His Thr acg tgc gca caa acg tgg Thr Cys Ala G n Thr Trp 75 gaa ttt gat cgg gtg att G u Phe Asp Arg Val Ile aat aac ctg gac acc tcc Asn Asn Leu Asp Thr Ser 105 atg cat gcc ggc gat gta Vet His Ala G y Asp Val 120 gct ccc tgg ata att gat Ala Pro Trp Ile Ile Asp 135 ctc acc cct gaa gaa atc Leu Thr Pro u u lie 150 155 tta aac gcg tca gag aaa Leu Asn Ala Ser G u Lys 170 tgg tat ctc tac gag cat Trp Tyr Leu Tyr G u His 185 ctt ccc tat cgc tgt gat Leu Pro Tyr Arg Cys Asp 200 gca ggc aga tct tcc aat Ala y Arg Ser Ser Asn 215 <210> 638 <211> 220 <212> PRT <213> Corynebacter i um gl <400> 638 Val G u Leu Ala Arg G y 1 5 Thr G y Lys Leu Thr Ser Asp Pro Ser IVet Asp IVet cag gtc G n Val tcc gcg Ser Al a 45 gga at a G y lle 60 cat tgg His Tr p gca cct Ala Pro atc gcg I e Al a ctc aag Leu Lys 125 cga gaa Arg G u 140 atc cag Ile G n atc aaa I e Lys ttg ggt Leu G y gca ttt Al a Phe 205 ctt tags Leu 220 ct a Leu 30 ctt Leu cgt Ar g gtt Val gag G u tgg Tr p 110 ccg Pr o at t SIle ct c Leu gag G u ttc Phe 190 tta gcc Al a ccg Pr o gac Asp gac Asp ggt Gy 95 gt a Val gga Gy cgc Ar g gct Al a cgt Ar g 175 agt Ser ctt ctt Leu gcg Al a aac Asn gt g Val gca Al a cac Hi s ttc Phe acc Thr cac Hi s 160 gtt Val ccc Pr o t ca gat cca Asp Pro gtt ccc Val Pr o gcg gtt Al a Val acg gct Thr Al a gtc ct g Val Leu cga ct c Arg Leu acc cca Thr Pro 130 acg tgg Thr Trp 145 acg agg Thr Arg gat cag Asp n gac aat Asp Asn cgt t cc Arg Ser 210 agc Ser tgc Cys gat Asp gcc Al a ct c Leu agt Ser 115 gaa G u aat Asn t cc Ser aac Asn cca Pr o 195 ggt Gy at g Met tgg Tr p ctg Leu t ca Ser gtg Val 100 cgc Ar g acc Thr cag G n t ac Tyr ctt Leu 180 gtg Val acc Thr Leu Leu Ser gagccct cgccatgtac ctg ut ari cum Phe y Arg Val Leu Asp Val y Ala y 10 G u Leu Thr Ala Asp G n Val Leu Ala Leu 25 Leu Arg Val Phe Arg Ser Ala Leu Pro Ala Page 788 seql i st cor r ect ed. t xt Ala G u His Thr Val Al a Thr Val Ar g Thr Thr 145 Thr Asp Asp Ar g Cys Asp Al a Leu Ser 115 G u Asn Ser Asn Pr o 195 Gy G n SIle Al a Tr p SIle Al a Hi s Tr p 165 G n G u Leu Al a Thr 70 G u Asn bet Al a Leu 150 Leu Tr p Leu Al a Thr Cys Phe Asn Hi s Pr o 135 Thr Asn Tyr Pr o Gy 215 Al a Asp Leu Al a 120 Tr p Pr o Al a Leu Tyr 200 Ar g n Ar g Asp 105 Gy I e Su Ser Tyr 185 Ar g Ser Tr p 75 I le Ser Val Asp SIle 155 Lys Hi s Asp Asn Gy Hi s Al a SIle Leu Ar g 140 I e I e Leu Al a Leu 220 li e Ar g Trp Val Pro G u Al a Tr p 110 Lys Pro 125 G u Ile G n Leu Lys G u G y Phe 190 Phe Leu 205 Asp Asp Gy Val Gy Ar g Al a Ar g 175 Ser Leu Asn Val Al a Hi s Phe Thr Hi s 160 Val Pr o Ser <210> 639 <211> 1392 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1369) <223> RXA00633 <400> 639 aaagctgcgg taattaaaaa cacttagcgc caaaaattga tacactgcag atatacatcc aaaccaagtg acggaggaaa ttg Leu ggc G y ct g Leu gca Al a gag G u cgc Ar g gat Asp cat Hi s gat Asp aga Ar g agc Ser cac Hi s cac Hi s ct c Leu acc Thr gga G y cga Ar g gt c Val gt g Val cac Hi s at c SIle aaa Lys gac Asp cga Ar g tgg cac Trp Hi s 15 acc gat Thr Asp gcg atg Al a lVet ctg aaa Leu Lys Page 789 acact gt t ca at t aacct at atg gaa aac ccc agc let G u Asn Pro Ser 1 ccg tat gcc gcg ccg Pro Tyr Ala Ala Pro gga gtg ttt ttg acg G y Val Phe Leu Thr agc tcc tgg tgg tcg Ser Ser Trp Trp Ser gct gcc gcc caa aaa Al a Al a Al a G n Lys seql i st cor r ect ed. t xt caa atc gac G n Ile Asp ccc gcc att Pro Al a l e ttt gac cac Phe Asp Hi s gcc atc aaa Al a I e Lys 120 cgg aca aaa Arg Thr Lys 135 acc gcg atg Thr Al a 'bet 150 aaa ggc aca Lys G y Thr ggg t ca t cg G y Ser Ser ctt atc gac Leu Ile Asp 200 ggc gct gga G y Al a G y 215 gcc aca ct g Al a Thr Leu 230 gcc act ggt Al a Thr G y ggc ct a caa G y Leu G n ttc atg tcc Phe Met Ser 280 atc agc acc Ile Ser Thr 295 at g gct aat Ibet Al a Asn 310 att gag acc Ile G u Thr acc atg agt cac gtc atg ttt Thr Met Ser His Val Met Phe 75 aag ctc acc cac aaa ctc ctc Lys Leu Thr His Lys Leu Leu 95 gtc ttt tat tcc gat tcg ggc Val Phe Tyr Ser Asp Ser G y 105 110 atg gca ctg cag gcc tcc aaa IVet Ala Leu G n Ala Ser Lys 125 ctc ctc acc tgg cgg tcc ggc Leu Leu Thr Trp Arg Ser y 140 agc gtg tgc gac cca gaa aat Ser Val Cys Asp Pro G u Asn 155 ctc ccc gag cag att ttc gcc Leu Pro u n lie Phe Ala 170 175 ccg cag gcg att tcc gag tac Pro G n Al a lie Ser G u Tyr 185 190 gag gcg gtc tcc gca atc at c Su Ala Val Ser Ala lle Ile 205 ggc atg cgc ttt cac gat gtc G y IVet Arg Phe His Asp Val 220 tgc aag aag cac gat cgt ttc Cys Lys Lys His Asp Arg Phe 235 ttc ggc cgc acc ggt gaa cta Phe G y Arg Thr y G u Leu 250 255 cca gac atc atg tgt gtg ggc Pro Asp Ile IVet Cys Val y 265 270 ttc gcc gct act tta tgc acg Phe Ala Ala Thr Leu Cys Thr 285 cca aat ggc gga ggt gcg ctg Pro Asn G y G y G y Ala Leu 300 cct ctg gcc tgt gcg gtt tcg Pro Leu Ala Cys Ala Val Ser 315 ggc atg tgg cag aaa cag gta Gy Iet Trp G n Lys G n Val 330 335 ggc G y 80 aat Asn tcg Ser gga G y t ac Tyr ggc G y 160 ccc Pr o ct g Leu at c SIle gca Al a ttg Leu 240 ttt Phe aag Lys gac Asp at g Met cat Hi s 320 aaa Lys gga ct a G y Leu acc cac ct c Leu gt c Val caa G n cac Hi s 145 at g Met gcc Al a cgc Ar g gaa G u ct c Leu 225 at c SIle gcc Al a gcc Al a aag Lys cac Hi s 305 gct Al a aga Ar g act Thr t ca Ser ggc G y 130 gga G y cat Hi s cca Pr o agc Ser ccg Pr o 210 at t SIle gt c Val acg Thr ct c Leu gt g Val 290 ggc G y t ca Ser at c SIle Thr gga G y gt g Val 115 cac Hi s gac Asp agc Ser cca Pr o at g Met 195 at c SIle gaa G u gat Asp tta Leu acc Thr 275 gct Al a ccc Pr o tta Leu gaa G u Hi s aat Asn 100 gag G u ccg Pr o aca Thr ct c Leu gtt Val 180 gaa G u gt c Val gga G y gaa G u agc Ser 260 ggt G y caa G n act Thr gaa G u gcc Al a 340 gag G u t cc Ser gt c Val gaa G u ttc Phe tgg Tr p 165 cgg Ar g ttg Leu caa G n gt c Val at t SIe 245 aat Asn gga G y tta Leu ttt Phe at c SIe 325 gaa G u 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 Page 790 ct t Le u gt c ValI aat As n cgc Ar g 390 t ca Ser ggg O31y seqI i st car r ect ed. t xt gca ggc ct t t cc cca ct t caa cac ct t cca ggg Ala O31y Leu Ser Pr o Leu O31n Hi s Leu Pro O31y 345 350 gt t ct c ggc gcg at t ggt gt c at c gaa at g gaa Val Leu (1y AlaIIe (1y Val IlIe (1u 'b t O31u 360 365 370 gaa gaa gct act cag gct gca t t a gat cac ggt G3 u G3 u A a Thr a3 n A a A a Leu Asp Hi s G3 y 380 385 t tt gga cgc t tg ct c t at gt c at g cct cca t at Phe O31y Ar g Leu Leu Tyr Val I'b t Pr o Pr o Tyr 395 400 cag t gc gca cag at c t gc act gcg ct t cat gct 03 n Cys A a 03 n IlIe Cys Thr A a Leu Hi s A a 410 415 t aaaccat gc cattt t tat t t gt gt t ValI 355 c aa O31n gt g ValI at c I Ie gca A a gcc A a aat As n t gg Tr p ac c Thr gt t ValI 420 1171 1219 1267 1315 1363 1392 <210O> 640 <21 1> 423 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 640 I'bt O31u As n Pro Se r Leu Arg O31u Leu His Arg Asn Ilie Tr p Hi s Pr o a31y Ser A a a31y Le u ValI O31n Hi s 145 vb t A a A a Ph e Tr p A a Thr a31y ValI 115 Hi s As p Ser Pr o Pr o Thr Ser Lys5 O31u Ser ValI O31u Ph e Tr p 1 65 Ar g a31y Le u A a O31n 70 Pr a Ph e A a Ar g Thr 150 Lys5 (31y ValI O31u I Ie li1e A a As p I Ie Thr 135 A a O31y Ser As n O31y O31y Thr Lys5 ValI 1 vb t Le u Ser Le u Pr a 1 85 Le u Thr O31y Ser Thr Tyr Le u Thr Cys 1 (31u Thr I Ie Pr a ValI Lys5 As p A a 125 Ar g Pr a I Ie Thr A a Le u Ph e Le u O31y O31y As n A a 1 O31n Ala I Ie Ser O31u Tyr Leu 1 Page 791 Ar g G u Leu 225 I le Al a Al a Lys Hi s 305 Al a Ar g Pr o bet Hi s 385 Pr o Hi s Ser IVet G u Leu Leu Ile 195 seql i st cor r ect ed. t xt p O u Ala Val Ser Ala lle Ile Ile 0 205 SIle G u Asp Leu Thr 275 Al a Pr o Leu G u Val 355 G n Val SIle Al a G n Val SIle 245 Asn Gy Leu Phe SIle 325 G u Asp Val SIle Thr 405 Lys Gy Al a 230 Al a Gy Phe SIle Met 310 I le Leu Val Asn Ar g 390 Ser Gy Al a 215 Thr Thr Leu bet Ser 295 Al a Su I e Ar g Val 375 Pr o G u Lys Gy Cys Phe Pr o 265 Phe Pr o Pr o Gy Gy 345 Leu G u Gy Cys bet Lys Gy 250 Asp Al a Asn Leu Met 330 Leu Gy Al a Ar g Al a 410 Ar g Lys 235 Ar g SIle Al a Gy Al a 315 Tr p Ser Al a Thr Leu 395 G n Hi s Asp Gy Cys Leu 285 Gy Al a Lys Leu Gy 365 Al a Tyr Cys Asp Ar g G u Val 270 Cys Al a Val G n G n 350 Val Al a Val Thr Val Phe Leu 255 Gy Thr Leu Ser Val 335 Hi s SIe Leu bet Al a 415 <210> 641 <211> 795 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(772) <223> RXA00632 <400> 641 tggacgcttg ctctatgtca tgcctccata tatcaccacg tcagagcagt gcgcacagat ctgcactgcg cttcatgctg cagttaaagg gaaataaacc atg cca ttt tta ttt IVet Pro Phe Leu Phe 1 gtc agc ggt acc gga act ggg gtt ggg aaa acc ttc tcc aca gcc gtt Val Ser y Thr y Thr y Val y Lys Thr Phe Ser Thr Ala Val 15 ttg gtt cga tac tta gcc gat caa gga cac gat gtt ctg ccc gta aag Page 792 Leu Val Arg Tyr seql i st cor r ect ed. t xt Leu Al a Asp n G y His Asp Val Leu 30 Pro Val Lys cta gtc caa acc ggt gaa ctt Leu Val G n Thr Gy G u Leu gaa cgc ttg act gga att gct Su Arg Leu Thr Gy Ile Al a 60 cct ctt gcg cca aat ctg gca Pro Leu Ala Pro Asn Leu Ala 75 cag ttt gat cag att atc tcg G n Phe Asp n Ile Ile Ser cgc atc att gtg gtg gag ggc Arg Ile Ile Val Val u y 105 gaa gat ttc acc ctg gca gat G u Asp Phe Thr Leu Al a Asp 120 gtg att gtg aca agc acc gga Val lie Val Thr Ser Thr G y 135 140 agc gtt gag gca gca aac cgc Ser Val G u Ala Ala Asn Arg 150 155 ggc ggt tcg atc cct caa aat Gy Gy Ser lie Pro n Asn 170 gaa gaa ttt gag aga gtc acc G u G u Phe G u Arg Val Thr 185 gaa ggg ttg tca cgg gtg gag G u Gy Leu Ser Arg Val G u 200 gcc ctt gat gcc ttt aag aaa Ala Leu Asp Ala Phe Lys Lys 215 220 tcg <210> 642 <211> 224 <212> PRT <213> Corynebacter i um gl ut a <400> 642 IVet Pro Phe Leu Phe Val Ser 1 5 Phe Ser Thr Ala Val Leu Val Val Leu Pro Val Lys Leu Val cca Pr o 45 gga Gy gcc Al a tgg Tr p gct Al a gtt Val 125 ttg Leu cga Ar g cct Pr o ggc G y ggg G y 205 ggc G y gag G u cga Ar g ctt Leu ggt Gy 110 gcc Al a gga G y gga G y gat Asp gt g Val 190 ttc Phe gag G u gaa G u cga Ar g cgt Ar g 95 ggc Gy t cc Ser agc Ser ct c Leu ct a Leu 175 ccc Pr o gt c Val gga G y ttt Phe gag G u ggt Gy ct g Leu gct Al a ct c Leu aca Thr 160 gct Al a ttt Phe gaa G u gac Asp gct Al a ggg Gy ttt Phe ct g Leu ttg Leu aac Asn 145 gt g Val acg Thr tgg Tr p aag Lys at c I e cgt Ar g gt c Val gac Asp gt c Val aat Asn 130 gct Al a ttg Leu at g Met gga G y caa G n 210 ttt Phe ttc Phe gag G u gac Asp aga Ar g 115 gca Al a gct Al a gga G y ctt Leu gct Al a 195 tct Ser aac Asn aaa Lys cca Pr o cca Pr o 100 tta Leu ccc Pr o gaa G u gtc Val aat Asn 180 ttg Leu ttt Phe ccg ccg gca agg Pro Pro Ala Arg t gat cgt gaa caccgt gcct 792 ri cum y Thr y Thr y Val y Lys Thr 10 Arg Tyr Leu Ala Asp G n Gy His Asp 25 G n Thr G y G u Leu Pro G y G u G y 40 Page 793 seql i st cor r ect ed. t xt Asp Ile Phe Asn lie u Arg Leu Thr G y Ile Al a Gy G u G u Phe Al a Sy Phe Leu Leu Asn 145 Val Thr Tr p Phe G u Asp Ar g 115 Al a Al a Sy Leu Al a 195 Asp SIle Asp Sy Leu Leu Leu 165 Leu Pr o Leu Phe SIle Asp SIle 135 Val Sy G u Sy Leu 215 Al a Asp SIle Phe 120 Val G u Ser Phe Leu 200 Pr o G n Val 105 Thr Thr Al a SIle G u 185 Ser Asn SIle 90 Val Leu Ser Al a Pr o 170 Ar g Ar g Leu 75 I e G u Al a Thr Asn 155 G n Val Val Al a Ser Sy Asp Sy 140 Ar g Asn Thr G u Al a Tr p Al a Val 125 Leu Ar g Pr o Gy Sy 205 Ar g Leu Sy 110 Al a Sy Sy Asp Val 190 Phe Ar g Ar g Sy Ser Ser Leu Leu 175 Pr o Val G u Sy Leu Al a Leu Thr 160 Al a Phe G u Lys G n 210 Ser Phe Pro Ala Asp Ala Phe Lys Pro Pro Ala Arg <210> 643 <211> 1125 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1102) <223> RXA00295 <400> 643 gtttaaggca cgtgtcattt tgcatgcgcg tgcccaattc ttaagcgcgc agttattgac aaccagcctc taggagatcc ttcttaagtg tgctcaattt atg acc atc ccc ggc acc Thr ctt Leu at c SIle ggg ctt Leu cag G n gac Asp gaa acc Thr cag G n at g Met gag gcc Al a tt g Leu gaa t u gt c acc Thr gag G u gcc Al a 45 ggc caa G n gtt Val cac Hi s at t IVet Thr Ile Pro Gy 1 gtt ctg gaa cag gga att ggc Val Leu Gu Gn Gy lie y 15 ctc acc ttg cct gaa gag caa Leu Thr Leu Pro u G u n cag gtt cgg ttg aag tgg tgt G n Val Arg Leu Lys Trp Cys att tcc ctc aaa act ggc ggt Page 794 seql i st cor r ect ed. t xt Gy Gu u u le Ou Val Ou Gy Ile Ile Ser Leu Lys 60 Thr G y G y tgc Cys ccg Pr o aaa Lys gt c Val ct c Leu acg Thr 150 cgc Ar g gt c Val gca Al a gaa G u gat Asp 230 cca Pr o at t SIle ggc Gy ct g Leu ggc 31y 310 gaa G u cgt Ar g acc Thr ggg Gy 120 at t SIle aat Asn aac Asn act Thr gct Al a 200 tta Leu cac Hi s gcc Al a gcg Al a cgc Ar g 280 ggc Gy cca Pr o tgt Cys gt g Val aaa Lys gat Asp tct Ser gaa G u aat Asn 170 aca Thr at g Met cag G n gt c Val agg Ar g 250 cgc Ar g ct g Leu aat Asn gaa G u cat Hi s 75 tgg Tr p act Thr gag G u gaa G u cag G n 155 tt g Leu tgg Tr p gaa G u cgc Ar g ccc Pr o 235 gaa G u ctt Leu act Thr gcg Al a gat Asp 315 ttc Phe ct g Leu ggc Gy agg Ar g gtt Val 140 gt g Val gaa G u gaa G u gt c Val gcc Al a 220 at g Met ttg Leu gcg Al a ttg Leu at g Met 300 gac Asp tgc Cys gat Asp gct Al a ct c Leu 125 gaa G u gat Asp act Thr gag G u tgt Cys 205 gag G u aac Asn at g Met at g Met ggc Gy 285 at c SIle ct c Leu t ca Ser at t SIle acc Thr 110 at g bet at t SIle cgc Ar g gcg Al a cgc Ar g 190 t cc Ser ttt Phe ttc Phe gac Asp cct Pr o 270 gac Asp gt c Val gac Asp cag tct G n Ser 80 ccg aat Pro Asn 95 gaa ttc G u Phe acc cag Thr G n gaa gt c G u Val ct c gct Leu Al a 160 cgt t cc Arg Ser 175 cgc gaa Arg u ggc gga G y G y gcc gtg Al a Val ctt gat Leu Asp 240 agc cgt Ser Arg 255 cac acc Hi s Thr aag ggt Lys G y gga aac Sy Asn at g at g vet vet 320 ggg G y ct g Leu tgt Cys ct g Leu gca Al a 145 gct Al a t at Tyr act Thr at c SIle cag G n 225 cct Pr o gac Asp at g Met t cc Ser t ac Tyr 305 gat Asp ttg Leu gtt Val at c SIle gag G u 130 gca Al a gcc Al a ttc Phe ttg Leu tta Leu 210 ct g Leu cgc Ar g gct Al a ctt Leu gag G u 290 ct g Leu cgt Ar g ttt Phe gaa G u gt c Val 115 gaa G u tcg Ser ggc Gy cct Pr o cgc Ar g 195 gga G y gcg Al a ccg Pr o ct g Leu cgt Ar g 275 caa G n act Thr ct c Leu gaa G u gcc Al a 100 gcc Al a gca Al a at c SIle gt g Val gaa G u 180 ct g Leu at g Met gag G u ggc G y cgc Ar g 260 ttt Phe gcc Al a acg Thr cag G n tcg Ser gct Al a gca Al a gt c Val gga G y cac Hi s 165 gtt Val gt g Val ggc G y ctt Leu acc Thr 245 tct Ser gct Al a ct c Leu ct c Leu ct g Leu 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1122 ccc atc aaa gtc ctt aat aag gtc atc taagaagcac gcgcatgaac Page 795 seqI i st car r ect ed. t xt Pro Ilie Lys Val Leu Asn Lys Val Ilie 330 g ac <210O> 644 <21 1> 334 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1125 <400> 644 I'bt Th r I Ie Pro O31u Le u Ar g Le u G31y Le u CYS Le u Al a 145 Al a Tyr Thr I Ie O31n 225 Pr a As p vb t Ser G31y O31u Lys Thr Ph e O31u ValI 115 O31u Ser G31y Pr a Ar g 1 95 G31y Al a Pr a Le u Ar g 275 (31n O31y O31y O31n cys O31y Ser Al a Al a ValI O31y Hi s 1 65 ValI ValI O31y Le u Thr 245 Ser Al a Le u As n Pr a O31u Pr a ValI O31n Ly s Al a 135 Le u Tyr Thr (31u Thr 215 Pr a Ph e G31y G31y (31y 295 O31n As p 40 O31u O31u Ar g Thr O31y 120 li1e As n As n Thr Al a 200 Le u Hi s Al a Al a Ar g 280 (31y Thr I Ie Leu Asp O31n Le u I Ie As p Ser Al a 1 Pr a Hi s Ly s Hi s Hi s 1 O31y O31u O31u As p Ph e 265 Ou le Al a Le u O31u ValI Hi s Tr p Thr O31u O31u O31n 1 Le u Tr p O31u Ar g Pr a 235 O31u Le u Thr Ar g vb t Le u O31u Ph e Le u O31y Ar g ValI 140 ValI O31u O31u ValI Al a 220 vb t Le u Al a Le u Thr O31u Al a O31y cys As p Al a Le u 125 O31u As p Thr O31u cys 205 O31u As n vb t vb t O31y 285 ValI Le u O31n I Ie O31n Pr a O31u Thr O31u Le u Ar g 1 Ar g O31y Al a Le u Se r 255 Hi s Ly s Asn Ala N/t lieVal cOy Asn 300 Page 796 seql i st cor r ect ed. t xt Tyr Leu Thr Thr Leu G y Arg Pro IVet G u Asp Asp Leu Asp IVet IVet 305 310 315 320 Asp Arg Leu G n Leu Pro lie Lys Val Leu Asn Lys Val lie 325 330 <210> 645 <211> 1212 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1189) <223> RXA00223 <400> 645 gcgacctctt tgacatcgcc cctgcgctca tcgaagagat tttgaacact ttttatctgg accatgcagc caccacacca caacaagcgc aagtaggagt atg cgt gag gtg gcc 11! lbt Arg O u Val Ala gca Al a t ac Tyr gaa G u gcg Al a gca Al a at t SIle ct c Leu gac Asp ggc G y ggc G y 150 gt c Val gcg Al a tcg Ser gct Al a ggc Gy cct Pr o gaa G u ccg Pr o cct Pr o 120 at t SIle cca Pr o ttt Phe at g Met cgt Ar g ttg Leu gag G u aat Asn gt c Val ggt Sy gcg Al a ccg Pr o cac Hi s gag Su 170 gaa G u aag Lys ct g Leu gca Al a cgg Ar g 75 aag Lys cca Pr o gtt Val gtt Val at c SIle 155 ct c Leu aac Asn gcg Al a ggc Gy gat Asp at t SIle gct Al a gat Asp gcc Al a tct Ser 140 gat Asp ggc Gy gcg Al a cgc Ar g tgt Cys 45 aac Asn at t SIle ct a Leu gga G y act Thr 125 gag G u gcg Al a gcc Al a cag G n agc Ser gaa G u ct c Leu tct Ser gaa G u cga Ar g 110 at g Met ttc Phe gtt Val acc Thr gca Al a 15 gtt Val cct Pr o gct Al a acg Thr ctt Leu 95 gt g Val at g Met at c SIle cag G n act Thr 175 ttg Leu gcg Al a at c SIle gt g Val ccg Pr o ggc G y tct Ser tgg Tr p gcc Al a gt c Val 160 ttg Leu 1 aat Asn gat Asp gag G u cag G n at c SIle ggg G y t cc Ser gcg Al a gcc Al a 145 gtt Val gct Al a ccc Pr o tcg Ser gtt Val ggg G y gag G u gca Al a ttc Phe aac Asn 130 gcg Al a ggc G y gcc Al a gcg Al a gct Al a gt g Val tta Leu cac Hi s gag G u gaa G u 115 aat Asn cag G n cat Hi s t cc Ser agt Ser cgt Ar g ttt Phe ttc Phe ccc Pr o gcg Al a 100 gcg Al a gag G u gcg Al a ctg Leu gcg Al a 180 Page 797 seql i st cor r ect ed. t xt aaa ttc ggt gga cca cgt ggc gtc ggc ctg ctg ttg gtg agg cgc tca 691 Lys Phe G y y Pro Arg y Val y Leu Leu Leu Val Arg Arg Ser 185 190 195 cca gca cct tca gcc gta ttg cac gga ggt ggt cag gag cgc ggc atc 739 Pro Ala Pro Ser Ala Val Leu His G y G y G y n Gu Arg y lie 200 205 210 cgt cca ggc acc ctt gat gtc gcc ggc gca gct gcc acc gca gcc gca 787 Arg Pro Gy Thr Leu Asp Val Ala Gy Ala Ala Ala Thr Ala Ala Ala 215 220 225 tta cgc gaa gca gtg gcc gag ctt gac ggc gaa gcc acc cgc ctg cgc 835 Leu Arg G u Ala Val Al a u Leu Asp Gy G u Ala Thr Arg Leu Arg 230 235 240 245 gga ctt aaa aag atg ctt ctc gac gcc atc ctc cac acc atc ccc aac 883 Gy Leu Lys Lys IVet Leu Leu Asp Ala lie Leu His Thr lie Pro Asn 250 255 260 gta ctg gtc cac acc acc gaa cca tcc ctg cca gga cac ctg cat ctc 931 Val Leu Val His Thr Thr G u Pro Ser Leu Pro Gy His Leu His Leu 265 270 275 tcc ttc cca gga gca gaa ggc gat agt ttg atc atg ctg ctc gac tcc 979 Ser Phe Pro Gy Ala Gu Gy Asp Ser Leu Ile IVet Leu Leu Asp Ser 280 285 290 ttg cgg atc gaa gcc tcc aca ggt tcg gcc tgc tcc aac ggt gta aac 1027 Leu Arg lie G u Ala Ser Thr y Ser Ala Cys Ser Asn y Val Asn 295 300 305 cgt gcc agc cac gtc ctt ttg gcc atg gga att tcc gaa acc gac gcc 1075 Arg Ala Ser His Val Leu Leu Ala IVet Gy Ile Ser G u Thr Asp Ala 310 315 320 325 cgt ggt gcc atc cga ttc acc ctc gga aga acc acc act gaa gaa tcc 1123 Arg y Ala lie Arg Phe Thr Leu y Arg Thr Thr Thr Gu u uSer 330 335 340 atc aag gca gtg atc gcc gtg atc gaa gac gta gtg acc agg gct cgt 1171 Ile Lys Ala Val Ile Al a Val Ile G u Asp Val Val Thr Arg Ala Arg 345 350 355 act gcg gga atg gct ttt tagcgaccgt aaatcgcata gtg 1212 Thr Al a G y IVet Al a Phe 360 <210> 646 <211> 363 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 646 IVet Arg u Val Ala Ala Ala Ala Trp IVet Gu Asn Ala Gn Ala Leu 1 5 10 Asn Pro Ala Ser G n Tyr y Ser y Arg Lys Ala Arg Ser Val Ala 25 Asp Ser Ala Arg Gu Gu I e Ala Ser Leu Leu y Cys u Pro lie 40 G u Val Val Phe Thr Al a Ser y Thr G u Ala Asp Asn Leu Ala Val 55 Page 798 seqI i st car r ect ed. t xt On Oy Leu Phe His Ala Ser Pro Leu Asn Arg Ilie 11 70 e Ser Thr Pr o I Ie G31y Ser A a A a 145 ValI A a Le u O31n A a 225 A a Hi s G31y vb t Ser 305 Ser Thr ValI Hi s O31u O31u 115 As n O31n Hi s Ser Ar g 1 95 Ar g A a Ar g I Ie Le u 275 Le u G31y Thr (31u Ar g 355 G31y (31u Le u Thr Ser Pr o 1 65 Hi s Ser I Ie A a Ar g 245 As n Le u Ser As n A a 325 Ser Ar g Le u vb t Ly s A a 135 Thr As n Ph e A a Pr o 215 Ar g Le u Le u Ph e Ar g 295 A a G31y Ly s A a (31u Pr o Pr o 120 li1e Pr a Ph e G31y Pr a 200 G31y (31u Ly s ValI Pr a 280 li1e Ser A a A a G31y 360 Thr I Ie 1 A a O31n Thr As p O31y 1 Ser Thr A a Lys5 Hi s 265 O31y O31u Hi s I Ie ValI 345 vb t ValI 90 O31y A a Pr a Hi s O31u 1 70 Pr a A a Le u ValI vb t 250 Thr A a A a ValI Ar g 330 li1e A a Lys5 Pr a ValI ValI I Ie 1 Le u Ar g ValI As p A a 235 Le u Thr O31u Ser Le u 315 Ph e A a Ph e A a As p A a Ser 140 As p O31y O31y Le u ValI 220 O31u Le u O31u O31y Thr 300 Le u Thr ValI Le u O31y Thr 125 O31u A a A a ValI Hi s 205 A a Le u As p Pr a As p 285 O31y A a Le u I Ie Le u ValI vb t I Ie O31n Thr 1 Le u O31y A a Oy le 255 Le u Le u A a O31y Ar g 335 As p <210O> 647 <21 1> 1197 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 OIDS <222> (1174) <223> RXN00262 Page 799 seql i st cor r ect ed. t xt <400> 647 acaccgcggg aaagattgca tcaaccggtg tcgacgtcat attctgtgca tgcacttgac ctaggactcg atattttcta ttccgttgga gcgcttaccc atg ctc tac ctt IVet Leu Tyr Leu aat Asn cct Pr o gga Gy cgc Ar g t ca Ser cgt Ar g gaa G u ct a Leu gca Al a gag G u 150 acg Thr ttg Leu aaa Lys cat Hi s gcg Al a 230 gcc Al a ct c Leu ct c Leu at c SIle gcc Al a cgg Ar g gct Al a ccc Pr o 120 agg Ar g gga G y ttt Phe gtt Val at t SIle 200 ggc Gy gct Al a acc Thr gga Gy tct Ser gga Gy aac Asn ct c Leu t at Tyr cac Hi s gac Asp at t SIle acc Thr 170 gcg Al a gt g Val cag G n gcc Al a agt Ser gcg Al a gcg Al a cgc Ar g ct c Leu 75 at c SIle ctt Leu act Thr acc Thr cag G n 155 gat Asp tta Leu tta Leu gaa G u ttt Phe 235 gt g Val ttt Phe ggg Gy ccc Pr o gct Al a acc Thr gaa G u ggg G y aca Thr 140 ccg Pr o gca Al a agt Ser tgg Tr p aaa Lys 220 gcc Al a cgc Ar g ggc Gy ct g Leu 45 aca Thr at c l Ie acc Thr agg Ar g ct g Leu 125 ttg Leu at a SIle gt g Val ttg Leu t ca Ser 205 ggg Gy act Thr aat Asn aat Asn gag G u cag G n aaa Lys ccg Pr o ttt Phe 110 at c SIle at c SIle gct Al a caa G n tcg Ser 190 aag Lys cgg Ar g gcc Al a gaa G u 15 ccg Pr o gat Asp gt g Val gga G y at c SIle 95 cat Hi s t cc Ser agc Ser gag G u gct Al a 175 ggt G y ctt Leu cgt Ar g ttg Leu gca Al a t ca Ser gct Al a acg Thr gcg Al a 80 gag G u gat Asp ccg Pr o at t SIle ttg Leu 160 gca Al a cat Hi s ccc Pr o agt Ser gaa G0u 240 1 ctt Leu agt Ser cga Ar g ttt Phe tgc Cys cat Hi s ttc Phe gag G u ggt G y 145 gcg Al a cat Hi s aaa Lys ct g Leu ggc G y 225 ttg Leu gag G u ccc Pr o act Thr acg Thr tta Leu gac Asp gag G u ggt G y 130 t at Tyr gcg Al a tta Leu ttc Phe gag G u 210 acg Thr gcc Al a gcc Al a cat Hi s cgg Ar g tcg Ser gct Al a agt Ser at c SIle 115 ct c Leu gcc Al a gt a Val acc Thr ggt G y 195 ccg Pr o gaa G u agg Ar g at g Met gag G u gt g Val ggt G y aat Asn gt c Val 100 acc Thr cgc Ar g aac Asn agc Ser ttt Phe 180 gcg Al a gt a Val aac Asn gcg Al a gat Asp tgg Tr p gtg Val gcc Al a gga G y cct Pr o ct a Leu t ac Tyr aaa Lys aat Asn agt Ser 165 gac Asp cct Pr o at c SIe gtt Val gaa 245 tcc tat cca gat ctt ggc gaa ttc atc gag gaa gtt ctc act atc ccg Page 800 seql i st cor r ect ed. t xt Ser Tyr Pro Asp Leu G y u Phe lie G u G u Val Leu Thr lie Pro 250 255 260 gga gca cac ctg act gga cat cct agg atg cgc att gat gga cac gca 931 Sy Ala His Leu Thr Gy His Pro Arg IVet Arg lie Asp G y His Ala 265 270 275 tct ttt ctc ttc gac agc ata gga tct gaa act gtt ctt ctg gaa ttg 979 Ser Phe Leu Phe Asp Ser Ile y Ser G u Thr Val Leu Leu G u Leu 280 285 290 gaa cgc caa ggc att gtg tgc tcc cct ggt tct gcc tgt ggt tcc gga 1027 Su Arg n Gy lie Val Cys Ser Pro y Ser Ala Cys y Ser y 295 300 305 gag gta tcc cat gtg ttg ctg gcg ttg ggg ctt gag gag gat caa gca 1075 G u Val Ser His Val Leu Leu Ala Leu y Leu G u G u Asp G n Ala 310 315 320 325 cga acg gct gtg cgc tgt act ttt agt aca aca cac agc cgt gaa gat 1123 Arg Thr Ala Val Arg Cys Thr Phe Ser Thr Thr His Ser Arg G u Asp 330 335 340 gcg ctc gtg gca gcc tct gct ctt aaa tcc gcg gtc gcc tta atc aga 1171 Ala Leu Val Ala Ala Ser Ala Leu Lys Ser Ala Val Ala Leu lie Arg 345 350 355 ggg tgacgctagt cagaggttta cgg 1197 Gy <210> 648 <211> 358 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 648 IVet Leu Tyr Leu Asp Asn Ala Ala Thr Thr Ser Val Arg Asn u Ala 1 5 10 Leu G u Ala IVet Trp Pro Tyr Leu Thr y Ala Phe Gy Asn Pro Ser 25 Ser Pro His G u Val G y Arg Leu Ala Ser Ala G y Leu G u Asp Ala 40 Arg Thr Arg Val Ala Arg Ile Ile Gy y Arg Pro Thr G n Val Thr 55 Phe Thr Ser G y G y Ser G u Al a Asn Asn Leu Al a lie Lys G y Al a 70 75 Cys Leu Ala Asn Pro Arg G y Arg His Leu IIe Thr Thr Pro lie G u 90 His Asp Ser Val Leu G u Thr Ala Ala Tyr Leu G u Arg Phe His Asp 100 105 110 Phe G u IIe Thr Tyr Leu Ser Pro Asp His Thr G y Leu lie Ser Pro 115 120 125 G u y Leu Arg Lys Al a Val Arg Pro Asp Thr Thr Leu I e Ser Ile 130 135 140 Sy Tyr Ala Asn Asn u Val y Thr lie On Pro lie Ala u Leu 145 150 155 160 Page 801 seql i st cor r ect ed. t xt Ala Ala Val Ser Ser Thr Pro Phe His Thr Asp Ala Val 165 170 G n Ala Ala 175 Hi s Lys Leu Gy 225 Leu Val SIle Val Al a 305 G u Hi s Val Thr Phe Asp Leu 180 Gy Ala Pro Lys 195 Pro Val Ile His Su Asn Val Ala 230 Arg Ala u Ser 245 Thr Ile Pro y 260 G y Hi s Al a Ser 275 Leu G u Leu G u G y Ser G y G u 310 Asp G n Ala Ar g 325 Arg G u Asp Al a 340 Leu lie Arg y 355 Sy Sy Sy 215 Sy Tyr Al a Phe Ar g 295 Val Thr Leu Val SIle 200 Sy Al a Pr o Hi s Leu 280 G n Ser Al a Asp 185 Sy Gy l e Asp Leu 265 Phe Sy Hi s Val Al a Val G n Al a Leu 250 Thr Asp SIle Val Ar g 330 Leu Leu G u Phe 235 Sy Sy Ser Val Leu 315 Cys Leu Ser 205 Sy Thr Phe Pr o Sy 285 Ser Al a Phe G y Hi s Leu Pro Arg Ser Leu G u 240 G u G u 255 Ibet Arg G u Thr G y Ser G y Leu 320 Thr Thr 335 Ser Al a Val Ala Ala Ser Ala Leu 345 <210> 649 <211> 920 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (79)..(897) <223> FRXA00262 <400> 649 cacacaggtg acgtttacgt cgggtggatc agaagccaac aacctcgctt atcaaaggag cgtgcttagc taatccta gtg gcc ggg cac ctc atc acc acc ccg atc gag Val Ala y His Leu Ile Thr Thr Pro Ile u 1 5 cat gac agt gtc cta gaa act gct gct tat ctt gaa agg ttt cat gat His Asp Ser Val Leu G u Thr Ala Ala Tyr Leu G u Arg Phe His Asp 20 ttc gag atc acc tac cta tcc ccc gat cac act ggg ctg atc tcc ccg Phe G u Ile Thr Tyr Leu Ser Pro Asp His Thr y Leu lie Ser Pro 35 gag ggt ctc cgc aaa gca gtc agg ccg gac acc aca ttg atc agc att G u G y Leu Arg Lys Ala Val Arg Pro Asp Thr Thr Leu Ile Ser lie 50 Page 802 seql i st cor r ect ed. t xt ggt Gy gcg Al a cat Hi s aaa Lys ct g Leu ggc G y 140 ttg Leu gtt Val at t SIle gtt Val gcc Al a 220 gag Gu cac Hi s gt c Val gcc Al a gt a Val acc Thr ggt Sy 110 ccg Pr o gaa Gu agg Ar g act Thr gga Gy 190 ct g Leu ggt Gy gat Asp cgt Ar g tta Leu 270 aat Asn agt Ser gac Asp cct Pr o at c SIle gtt Val gaa Gu 160 ccg Pr o gca Al a ttg Leu gga Gy gca Al a 240 gat Asp gag gtg gga acc G u Val y Thr 65 acg cct ttt cac Thr Pro Phe His ttg gga gtt gac Leu Gy Val Asp 100 aaa ggg att gga Lys G y lle G y 115 cat ggc ggc ggc His G y G y G y 130 gcg ggg gct atc Al a G y Al a l le 145 tcc tat cca gat Ser Tyr Pro Asp gga gca cac ctg G y Al a His Leu 180 tct ttt ctc ttc Ser Phe Leu Phe 195 gaa cgc caa ggc G u Arg n y 210 gag gt a t cc cat G u Val Ser His 225 cga acg gct gtg Arg Thr Ala Val gcg ctc gtg gca Ala Leu Val Ala att cag ccg ata Ile n Pro Ile 70 gat Asp tta Leu tta Leu gaa G u ttt Phe 150 ggc G y gga G y agc Ser gt g Val ttg Leu 230 tgt Cys tct Ser gca Al a agt Ser tgg Tr p aaa Lys 135 gcc Al a gaa G u cat Hi s at a SIle tgc Cys 215 ct g Leu act Thr gct Al a gt g Val ttg Leu t ca Ser 120 ggg G y act Thr ttc Phe cct Pr o gga G y 200 t cc Ser gcg Al a ttt Phe ctt Leu gct Al a caa G n tcg Ser 105 aag Lys cgg Ar g gcc Al a at c SIle agg Ar g 185 tct Ser cct Pr o ttg Leu agt Ser aaa Lys 265 gag G u gct Al a ggt G y ctt Leu cgt Ar g ttg Leu gag G u 170 at g Met gaa G u ggt G y ggg G y aca Thr 250 t cc Ser ttg Leu gca Al a cat Hi s ccc Pr o agt Ser gaa G u 155 gaa G u cgc Ar g act Thr tct Ser ctt Leu 235 aca Thr gcg Al a atc aga ggg lie Arg G y tgacgctagt cagaggttta cgg <210> 650 <211> 273 <212> PRT <213> Corynebacter i um gl utam cum <400> 650 Val Ala y His Leu I e Thr Thr Pro IIe G u His Asp Ser Val Leu 1 5 10 G u Thr Ala Ala Tyr Leu u Arg Phe His Asp Phe u Ile Thr Tyr Page 803 seqi i st car r ect ed. t xt Asp Hi sThr 0 y 25 li1e Ser Pr o (31u Le u A a O31u Thr Le u Ly s Hi s A a 145 Ser G31y Ser O31u O31u 225 Ar g A a G31y Pr o Ar g G31y Ph e ValI I Ie 115 G31y A a Pr o Hi s Le u 1 95 O31n Ser A a ValI As p I Ie Thr A a ValI O31n A a Le u 1 65 Thr As p I Ie ValI Ar g 245 A a Thr Pr o A a Ser Tr p Ly s 135 A a O31u Hi s I Ie cys 215 Le u Thr A a Le u 40 Le u I Ie ValI Le u Ser 120 G31y Thr Ph e Pr o G31y 200 Ser A a Ph e Le u I Ie A a O31n Ser 1 05 Ly s Ar g A a I Ie Ar g 1 85 Ser Pr a Le u Ser Ly s 265 I Ie Le u A a Hi s Pr a Ser (31u 1 (31u Ar g Thr Ser Le u 235 Thr A a G31y A a Hi s Ly s Le u G31y 140 Le u ValI I Ie ValI A a 220 (31u Hi s ValI G31y Tyr A a Le u Ph e (31u 125 Thr A a Le u As p Le u 205 cys (31u Ser A a A a ValI Thr G31y 110 Pr a (31u Ar g Thr G31y 1 Le u G31y As p Ar g Le u 270 Leu Arg Lys As n Ser Ph e A a ValI As n A a I Ie 1 Hi s O31u Ser O31n O31u 255 li1e <210O> 651 <21 1> 1296 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1273) <223> RXN00435 <400> 651 cgacaggt ga at t cat gcac gt t t gagt gt cccgt gt gt g gggt aat gt t gt ccaagaga gt ggaaggaa at gct gt ggc ggt tgaaagg agt gcct t tc gt g ggt t tt gat gt g 115 Val 03 y Phe Asp Val 1 Page 804 gcc agg gtt cgg ggg Ala Arg Val Arg Gy seqi i st car r ect ed. t xt ctt tat acc tct ttg ggc gat ggc tgg acg tac Leu Tyr Thr Ser Leu G31y Asp G31y Tr p Thr Tyr ct t Le u gt t ValI c ag O31n t cg Ser c ag O31n cga Ar g gcg A a tt g Le u ggc G31y 1 50 ac g Thr cgt Ar g ct g Le u gag O31u at g vb t 230 t cc Ser t ca Ser gcg A a at t I Ie gcg A a tt g Le u gcg A a tt c Ph e 120 ac c Thr ac g Thr gcc A a t gg Tr p ct a Le u 200 ggc G31y ccg Pr o ccg Pr o c aa O31n tt c Ph e gt g ValI gt g ValI cat Hi s gt g ValI t cc Ser at g vb t ct t Le u gt g ValI 1 70 ct t Le u gag O31u ccg Pr o ct g Le u ggg G31y 250 ccg Pr o ac g Thr c ag O31n ccg Pr o tt g Le u tt g Le u g ac As p ccg Pr a 140 gt g ValI aag Ly s g ac As p g aa O31u at t I Ie 220 cgc Ar g ct c Le u c ag O31n cat Hi s 45 tt g Le u g ac As p gcg A a t cg Ser ggc G31y 125 g at As p ct c Le u at t I Ie gcc A a gcc A a 205 t cg Ser ac c Thr ggc G31y gt t ValI gcg A a gag O31u t gt cys cgc Ar g cgc Ar g 110 gt t ValI t gg Tr p ag c Ser gt g ValI ac c Thr 1 90 g at As p gcg A a gt t ValI G31y ccg Pr o c ag O31n gct A a gt t ValI ggt O31y 95 gcg A a tt t Ph e c ag O31n gcc A a g at As p 1 75 ac c Thr at c I Ie tt g Le u cca Pr a gt g ValI 255 t cc Ser gag (31u at t I Ie gct A a gt g ValI 80 tt g Le u g ac As p ag c Ser t ac Tyr gcg A a 1 60 aaa Ly s t ac Tyr gt c ValI at t I Ie ct c Le u 240 ccc Pr a gt c ValI cgg Ar g t ct Ser cgc Ar g 65 ct g Le u ggt O31y gcg A a t gg Tr p gag O31u 145 c ac Hi s gt g ValI gca A a at g vb t tt c Ph e 225 g aa O31u aac As n gt g ValI gag O31u 50 gag O31u ggt O31y ggg O31y g ac As p gcc A a 130 aag Ly s ccg Pr a Ar g gcc A a ct t Le u 210 cgt Ar g ct g Le u ct g Le u gcg A a 35 gt g ValI gca A a ccc Pr a tt t Ph e t gg Tr p 115 gag O31u ct t Le u ct g Le u gcg A a t ac Tyr 1 g at As p g at As p ccc Pr a gt g ValI t cg Ser ac g Thr gt t ValI ac g Thr gt a ValI 1 00 ct g Le u ccc Pr a gt t ValI ct c Le u cgt Ar g 1 cgc Ar g ct c Le u ac c Thr gca A a cgg Ar g 260 gga (31y t cg Ser gcg A a ag g Ar g cgt Ar g ac c Thr g at As p g ac As p ggc O31y 1 t cg Ser ccc Pr a ggc O31y t cg Ser ag c Ser 245 c ac Hi s ct g gga aac Leu 03 y Asn ct g gat gaa aac gcc ccg Leu Asp 03 u Asn A a Pro 265 270 gt t gag gcg at g ggg Val 3u Ala l'bt Oy 275 Page 805 seql i st cor r ect ed. t xt gag atg gcg aaa ttc cac aag gga ctt ttt gag cat ctt gtg gaa tcg 979 G u Met Ala Lys Phe His Lys G y Leu Phe G u His Leu Val G u Ser 280 285 290 ctc gaa gga ctt cac gcg gtg cat atc gtg gga att tcc ggc gat gcc 1027 Leu G u G y Leu His Ala Val His IIe Val G y IIe Ser G y Asp Ala 295 300 305 gca ggt caa gac gcc ccg ttc ctg gat cga gtg ccc cgc ttg acc ttc 1075 Ala Gy G n Asp Ala Pro Phe Leu Asp Arg Val Pro Arg Leu Thr Phe 310 315 320 325 acc atg gaa ggc gtg ccc gca gat atg gtg tac cgc cga ttg gtg gac 1123 Thr IVet G u y Val Pro Ala Asp IVet Val Tyr Arg Arg Leu Val Asp 330 335 340 aat cgt ttg atc act acc gtc agc cct gct gac ccg ctg ctc gaa gca 1171 Asn Arg Leu Ile Thr Thr Val Ser Pro Ala Asp Pro Leu Leu G u Ala 345 350 355 atg ggt gtg act gaa gct ggc gga tcg atc act atc gga cta agc ccg 1219 IVet y Val Thr u Ala Gy y Ser Ile Thr Ile y Leu Ser Pro 360 365 370 ttt agc acc tac tat gaa gtg gat cag ctg acc agg gtg ctg gca tcg 1267 Phe Ser Thr Tyr Tyr G u Val Asp G n Leu Thr Arg Val Leu Al a Ser 375 380 385 ctt gcc taaaccgcaa gcacgagctt gcc 1296 Leu Al a 390 <210> 652 <211> 391 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 652 Val y Phe Asp Val Al a Arg Val Arg y Leu Tyr Thr Ser Leu y 1 5 10 Asp G y Trp Thr Tyr Leu Asn Ser His n I I e Pro G n Val Pro G u 25 Arg Val Ala Ser G y Val Ala Ala Ala Phe Arg Thr His Ala n I I e 40 Ser G u Val Thr Ser G n Pro Ile Ala Val Asp G n Leu G u Ala Ala 55 Arg G u Ala Val Ala Ser Leu Ala y Val Asp Pro Asp Cys Val Val 70 75 Leu G y Pro Thr Arg G n Phe Leu Ala His Thr Leu Ala Arg G y Leu 90 Sy y Phe Val Arg Arg Lys Ala y Val Val Leu Ser Arg Ala Asp 100 105 110 Ala Asp Trp Leu Thr Al a Pro Phe Arg Ser Leu Asp y Val Phe Ser 115 120 125 Trp Ala G u Pro Asp Leu y Thr y IVet Leu Pro Asp Trp G n Tyr 130 135 140 G u Lys Leu Val Asp G y Ser Thr Arg Leu Val Val Leu Ser Ala Ala 145 150 155 160 Page 806 seqI i st car r ect ed. t xt Hi s Pr o Leu Leu ValI Al a Ivbt Ph e 225 (31u As n ValI Hi s I Ie 305 Pr o Ar g Pr o I Ie Ar g 385 Al a Tyr 1 95 As p As p Pr o ValI Al a 275 ValI G31y Le u Le u Le u 355 Le u Le u G31y 1 65 Ser Pr o G31y Ser Ser 245 Hi s G31y Ser Al a Ph e 325 As p Al a Pr o Ser Thr Val Ala Pro Val Gy Lys lieVal Al a Ar g Le u 215 Ph e Le u G31y vb t G u 295 G31y vb t Ar g G31y Ser 375 Al a ValI 1 As p G31y Ar g Hi s Le u 265 Lys5 Le u As p Gy le 345 Th r Ty r ValI Tr p O31n As p 235 Le u G31u Hi s Al a Pr a 315 Pr a Th r Al a a u Al a Al a 205 Ser Th r G31y Al a G31y 285 Hi s Le u As p Ser G31y 365 As p Asp Lys 1 Thr Tyr I e Val Leu I I e Pro Leu 240 Val Pr o 255 Ser Val Phe 03 u Val G y Ar g Val 320 Val Ty r 335 Al a As p I I e Thr Leu Thr <210O> 653 <21 1> 638 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (615) <223> FRXA00435 <400> 653 gt c gac gcc acc acc t ac gca gcc t ac cgc ccc ct g cgc ct a gac gag Val As p Al a Th r Thr Tyr Al a Al a Tyr Ar g Pro Leu Arg Leu Asp G31u 1 5 10 t gg gaa gcc gat at c gt c at g ct t gat ct c ggc gag t tg ggc ggc ccg Tr p (31u Al a Asp Ilie Val N/b t Leu Asp Leu G31y (31u Leu G31y G31y Pr o 25 cag at t t cg gcgt t gat t t t ccgt gat acc tcg at gt t cccg cgc ct g O31n I Ie Ser Al a Leu Ilie Phe Ar g Asp Thr Ser I'bt Phe Pr o Ar g Leu Page 807 seql i st cor r ect ed. t xt gat cgc acc gtt Asp Arg Thr Val ctg ctc ggc ggc Leu Leu G y G y gaa aac gcc ccg G u Asn Ala Pro cac aag gga ctt Hi s Lys G y Leu 100 gcg gtg cat atc Ala Val His Ile 115 ccg ttc ctg gat Pro Phe Leu Asp 130 ccc gca gat atg Pro Ala Asp IVet 145 acc gtc agc cct Thr Val Ser Pro gct ggc gga tcg Al a G y G y Ser 180 gaa gtg gat cag Su Val Asp n 195 gcacgagctt gcc <210> 654 <211> 205 <212> PRT <213> Corynebact <400> 654 Val Asp Al a Thr 1 Trp G u Ala Asp G n I e Ser Ala Asp Arg Thr Val Leu Leu G y G y G u Asn Ala Pro cca Pr o gt g Val t cc Ser ttt Phe gt g Val cga Ar g gt g Val gct Al a 165 at c SIle gaa G u 55 aac Asn gtt Val cat Hi s at t SIle ccc Pr o 135 cgc Ar g ccg Pr o at c SIle ct g Leu ct g Leu gag G u ctt Leu t cc Ser 120 cgc Ar g cga Ar g ct g Leu gga G y ccc Pr o gt g Val gcg Al a gt g Val 105 ggc Sy ttg Leu ttg Leu ct c Leu ct a Leu 185 gca Al a cgg Ar g at g Met 90 gaa G u gat Asp acc Thr gt g Val gaa G u 170 agc Ser agc Ser cac Hi s 75 ggg Sy tcg Ser gcc Al a ttc Phe gac Asp 155 gca Al a ccg Pr o t cc Ser ct g Leu gag G u ct c Leu gca Al a acc Thr 140 aat Asn at g Met tt t ttt Phe ct g Leu gga Sy at g Met gaa G u ggt Sy 125 at g Met cgt Ar g ggt G y agc Ser ccg Pr o aac Asn gcg Al a gga Sy 110 caa G n gaa G u ttg Leu gt g Val acc Thr 190 cat Hi s ct g Leu aaa Lys ctt Leu gac Asp ggc Sy at c SIle act Thr 175 t ac Tyr ggg G y gat Asp ttc Phe cac Hi s gcc Al a gtg Val act Thr 160 gaa G u t at Tyr ctg acc agg gtg ctg gca tcg ctt gcc Leu Thr Arg Val Leu Ala Ser Leu Ala 200 205 taaaccgcaa er i um gl ut ari cum Thr Tyr Ala Ala Tyr 5 lie Val IVet Leu Asp Leu Ile Phe Arg Asp 40 Pro Leu G u Leu Pro Val Pro Asn Leu Val 70 Ser Val Val G u Ala Arg Pro I 10 Leu G y I Thr Ser I Al a Ser Arg Hi s I M\t G y 90 Page 808 Ar g Leu Phe Leu G y Met Asp Sy Ar g Hi s Leu Lys Hi s Al a Pr o Pr o 145 Thr Al a O31u G31y Hi s 115 Le u As p Ser G31y As p 1 95 Ph e ValI Ar g ValI Al a 1 65 li1e Le u Hi s I Ie Pr o 135 Ar g Pr o I Ie Ar g seql i st car r ect ed. t xt Leu Val O31u Ser Leu O31u 1 Ser G3 y Asp Al a Al a a3 y 120 125 Ar g Leu Thr Phe Thr l'b t 140 Arg Leu Val Asp Asn Arg 1 Leu Leu O31u Al a 'b t G31y 1 70 G31y Leu Ser Pr o Phe Ser 1 Val Leu Ala Ser Leu Ala 200 205 Le u As p G31y I Ie Th r 1 Ty r <210O> 655 <21 1> 535 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (535) <223> FRXA02801 <400> 655 cgacaggt ga at t cat gcac gt t t gagt gt cccgt gt gt g gt ggaaggaa at gct gt ggc ggt t gaaagg agt gcct t t c gggt aat gt t gt ccaagaga ggt t tt gat G3 y Phe Asp gcc Al a ct t Le u gt t ValI c ag O31n t cg Ser c ag O31n cga Ar g gt t ValI t ca Ser gcg Al a at t I Ie gcg Al a tt g Le u gcg Al a ggg G31y c aa O31n tt c Ph e gt g ValI gt g ValI cat Hi s gt g ValI ct t Le u at t SI e cgc Ar g g at As p g at As p 75 aca Thr gt g ValI t at Tyr ccg Pr a ac g Thr c ag O31n ccg Pr a tt g Le u tt g Le u ac c Thr c ag O31n cat Hi s 45 tt g Le u g ac As p gcg Al a t cg Ser t ct Ser gt t ValI gcg Al a gag O31u t gt cys cgc Ar g cgc Ar g 110 ggc O31y gag O31u at t I Ie gct Al a gt g ValI tt g Le u g ac As p ggc O31y gt g ValI gag O31u gag O31u ggt O31y ggg O31y g ac As p t gg Tr p gcg Al a gt g ValI gca Al a CCC Pr a tt t Ph e t gg Tr p 115 ac g Thr t cg Ser ac g Thr gt t ValI ac g Th r gt a ValI 1 00 ct g Le u Page 809 seql i st cor r ect ed. t xt gcg ccg ttc cgc tcc ctc gac ggc gtt ttt agc tgg gcc gag ccc gat 499 Ala Pro Phe Arg Ser Leu Asp y Val Phe Ser Trp Ala G u Pro Asp 120 125 130 ttg ggc acc ggc atg ctg ccg gat tgg cag tac cag 535 Leu G y Thr G y Iet Leu Pro Asp Trp G n Tyr G n 135 140 145 <210> 656 <211> 145 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 656 Val y Phe Asp Val Al a Arg Val Arg y Leu Tyr Thr Ser Leu y 1 5 10 Asp G y Trp Thr Tyr Leu Asn Ser His n I I e Pro G n Val Pro G u 25 Arg Val Ala Ser G y Val Ala Ala Ala Phe Arg Thr His Ala n I I e 40 Ser G u Val Thr Ser G n Pro Ile Ala Val Asp G n Leu G u Ala Ala 55 Arg G u Ala Val Ala Ser Leu Ala y Val Asp Pro Asp Cys Val Val 70 75 Leu G y Pro Thr Arg G n Phe Leu Ala His Thr Leu Ala Arg G y Leu 90 Gy y Phe Val Arg Arg Lys Ala y Val Val Leu Ser Arg Ala Asp 100 105 110 Ala Asp Trp Leu Thr Al a Pro Phe Arg Ser Leu Asp y Val Phe Ser 115 120 125 Trp Ala G u Pro Asp Leu y Thr Gy IVet Leu Pro Asp Trp G n Tyr 130 135 140 G n 145 <210> 657 <211> 1386 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1363) <223> RXA02516 <400> 657 ttcattcacg ttttcgcgaa tggccagatt gtgaccaccg gtggcgctga gcttgctgac aagctcgagg ctgacggcta cgaccagttc atcaagtaac atg tcc gat ttc ctc 115 Iet Ser Asp Phe Leu 1 aat gca gat gga tcc ctc aat gtg gat aag gtg cgg gaa gaa ttc cca 163 Asn Ala Asp y Ser Leu Asn Val Asp Lys Val Arg G u G u Phe Pro 15 atc ctg aag cgc act gtt agg gat ggg aaa ccg ctt gct tac ctg gac 211 Page 810 Ile Leu Lys Arg Thr Val Arg Asp eql i st cor r ect ed. t xt Sy Lys Pro Leu Al a Tyr Leu Asp t ca Ser cac Hi s ct g Leu gct Al a gca Al a t cc Ser ct a Leu acc Thr 150 gat Asp act Thr gtt Val cag G n ttc Phe 230 gtt Val act Thr gct Al a gcg Al a gt g Val gag G u ttt Phe gaa G u aag Lys 120 cac Hi s gcg Al a gat Asp cag G n cgt Ar g 200 gtt Val gca Al a t at Tyr ggt G y gca Al a 280 tcg Ser cac Hi s gca Al a ggt G y ct c Leu cgt Ar g gca Al a ttg Leu ct c Leu 170 aat Asn aag Lys cat Hi s tct Ser aag Lys 250 at g Met caa G n cga Ar g aac Asn gat Asp gag G u ctt Leu cag G n ttg Leu 140 tgg Tr p ctt Leu acc Thr gt c Val cca Pr o 220 cat Hi s cca Pr o gaa G u ttt Phe ccc Pr o 45 gcc Al a gct Al a cag G n gtt Val gcc Al a 125 gt g Val t ac Tyr gat Asp ggt G y ggc G y 205 gt g Val aag Lys at c I le gtt Val gag G5u 285 gag G u ccc Pr o t at Tyr cat Hi s gcg Al a 110 ggg cca Pr o aag Lys gaa G u gct Al a 190 gct Al a aat Asn at g Met ttg Leu gt c Val 270 gcc Al a cgg Ar g gt g Val gaa G u gaa G u 95 t ac Tyr gat Asp tgg Tr p gt g Val act Thr 175 gt g Val ct c Leu ttc Phe ct g Leu gat Asp 255 acc Thr ggc G y gt g Val cac Hi s ggt G y at t SIle acc Thr acc Thr cag G n act Thr 160 gt c Val gct Al a acg Thr cac Hi s gga G y 240 gaa G u at g Met acg Thr tgg Tr p cgc Ar g gcc Al a gcg Al a ttg Leu gt g Val gag G u 145 gaa G u aag Lys gat Asp gt g Val gag G u 225 cct Pr o ct g Leu gag G u cag G n cgt Ar g ggt G y cgc Ar g ttc Phe ggt G y gt c Val 130 ct g Leu gat Asp gt c Val gtt Val ctt Leu 210 ct g Leu gca Al a cca Pr o ggt G y at g Met 290 gca Al a gcc Al a gag G u act Thr gat Asp 115 at c SIle tgc Cys ggt G y gtt Val cca Pr o 195 gat Asp gat Asp ggc G y cca Pr o t cc Ser 275 acc Thr gag G u t ac Tyr aag Lys aag Lys 100 gac Asp acg Thr cgt Ar g cgc Ar g gcc Al a 180 gag G u gcg Al a gta Val gtg Val ttt Phe 260 acc Thr agc Ser gtt gtg ggc ttg ggt gct gcc gtg gac atg ctg aat gaa atc ggt atg Page 811 1027 seql i st cor r ect ed. t xt Val Val G y Leu G y Al a Al a Val Asp Iet Leu Asn G u I le y Iet 295 300 305 gaa gca atc gca gcg cat gag cac gca ttg act gct tac gcg ttg gaa 1075 G u Al a le Al a Al a Hi s G u Hi s Ala Leu Thr Ala Tyr Ala Leu G u 310 315 320 325 aag ctc acg gca att aag gga cta acc att gct ggt cct ttg act gca 1123 Lys Leu Thr Ala lie Lys y Leu Thr lie Ala y Pro Leu Thr Ala 330 335 340 gag cag cgc ggc ggt gca atc agc ttc ggt gtc gag ggc att cac cca 1171 G u n Arg G y G y Ala lle Ser Phe G y Val G u G y Ile His Pro 345 350 355 cac gat cta ggc caa gtg ctt gac gat cag ggc gtg aat atc cgc gtc 1219 Hi s Asp Leu G y G n Val Leu Asp Asp G n y Val Asn lle Arg Val 360 365 370 ggc cac cac tgc gcg tgg ccc gtg cac cgc agc atg aac gta caa tcg 1267 G y Hi s Hi s Cys Al a Trp Pro Val His Arg Ser IVet Asn Val G n Ser 375 380 385 aca gca aga gca tct ttc tat ctc tat aac acc ttc gaa gaa atc gac 1315 Thr Ala Arg Ala Ser Phe Tyr Leu Tyr Asn Thr Phe G u G u lie Asp 390 395 400 405 cgc ctc gcg gca gcg atc gag aag gcc aag caa ttc ttt gga gtt gag 1363 Arg Leu Ala Ala Ala IIe G u Lys Ala Lys G n Phe Phe Gy Val G u 410 415 420 taatgaacct tgagcagatg tac 1386 <210> 658 <211> 421 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 658 IVet Ser Asp Phe Leu Asn Al a Asp G y Ser Leu Asn Val Asp Lys Val 1 5 10 Arg G u u Phe Pro Ile Leu Lys Arg Thr Val Arg Asp y Lys Pro 25 Leu Ala Tyr Leu Asp Ser y Ala Thr Ser G n Arg Pro G u Arg Val 40 Trp Arg Ala G u G u His Phe Val Leu His Thr Asn Ala Pro Val His 55 Arg y Ala Tyr n Leu Ala u u Ala Thr Asp Ala Tyr u y 70 75 Ala Arg G u Lys IIe Ala Ala Phe Val G y Al a u G n Hi s u IIe 90 Ala Phe Thr Lys Asn Al a Thr G u Ala Leu Asn Leu Val Ala Tyr Thr 100 105 110 Leu y Asp Asp Arg Ser y Lys Tyr Arg Val G n Ala y Asp Thr 115 120 125 Val Val lie Thr G u Leu G u His His Ala Asn Leu Val Pro Trp G n 130 135 140 G u Leu Cys Arg Arg Thr y Ala Thr Leu Lys Trp Tyr Lys Val Thr Page 812 seqi i st car r ect ed. t xt 150 Asp Leu Asp Ser O31u Ly s As p ValI O31u 225 Pr o Le u O31u O31n As n 305 Al a G31y O31u ValI vb t 385 Ph e Ph e G31y ValI Pr o 1 95 As p As p G31y Pr o Ser 275 Thr I Ie Al a Le u I Ie 355 li1e ValI O31u G31y I Ie 1 65 Ph e Le u cys As p O31y 245 Le u Tyr O31n vb t O31u 325 Al a Pr o ValI Ser As p 405 O31u Hi s Ar g Ser 215 Ser ValI O31y Al a ValI 295 Al a Le u O31n As p Hi s 375 Al a (31n Ar g 200 ValI Al a Tyr G31y Al a 280 G31y I Ie Thr Ar g Le u 360 Hi s Ar g Ser 1 85 Al a Pr a Ph e Al a Ser 265 Pr a Le u Al a Al a G31y 345 G31y cys Al a Le u 1 70 As n Ly s Hi s Ser Ly s 250 vb t O31n O31y Al a I Ie 330 O31y O31n Al a Ser Al a 410 (3 u Leu Asp 03 u Thr Val ValI Al a vb t O31y 235 Ser I Ie Ar g Al a Hi s 315 Ly s Al a ValI Tr p Ph e 395 G31y O31y 205 ValI Ly s I Ie ValI O31u 285 ValI Hi s Le u Ser As p 365 ValI Le u Al a 1 Al a As n vb t Le u ValI 270 Al a As p Al a Thr Ph e 350 As p Hi s Tyr 1 ValI Le u Ph e Le u As p 255 Thr O31y vb t Le u I Ie 335 O31y O31n Ar g As n Leu Al a Al a I e 0 u Lys Al a Lys 03 n 415 <210O> 659 <21 1> 570 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (547) <223> RXA02517 <400> 659 cgacagcaag agcatct t tc t at ct ctata acacct tcga agaaat cgac cgcct cgcgg cagcgat cga gaaggccaag caat tct t tg gagt tgagt a at g aac ct t gag cag 115 Page 813 seqi i st car r ect ed. t xt l'bt As n Leu G3 u G3 n at g O31y t gt cys ac c Thr gcc A a aag Ly s ggt O31y t cc Ser t gg Tr p c ag O31n Ar g g ac As p gag O31u ac g Thr ct g Le u tt t Ph e gt c ValI 120 gct A a gt g ValI cct Pr o tt g Le u gt c ValI gt t ValI aag Ly s ggc G31y aag Ly s c ag O31n at c I Ie t t c Ph e act Thr t cc Ser at g vb t 75 ct c Le u g at As p t ac Tyr gcg A a ct g Le u g at As p ct g Le u t ac Tyr gcc A a ac a Thr g aa O31u ccg Pr o gca A a 140 g ac As p gct A a cgc Ar g 45 g aa O31u gag O31u g aa O31u g at As p gca A a 125 ac c Thr c ac Hi s gag O31u gt g ValI gca A a gag O31u tt t Ph e ct c Le u 110 cgc Ar g gct A a aaa Ly s c ac Hi s ct g Le u ggt G31y gt g ValI aag Ly s gga G31y aag Ly s gct A a cca Pr o gt c ValI gag O31u t ca Ser c aa O31n at c G31y gcg A a 130 gcg A a c ac Hi s cct Pr o G31y ag c Ser gt c ValI t cc Ser 1 00 gct A a ct t Le u gca A a t agcccgct g t at taat tgg agg <210O> 660 <211> 149 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 660 l'bt Asn Leu 0 u On l'bt Tyr On 0u 1 5 Asn Pro O1n His Lys Gy Leu Arg Asp 25 Hi s Val As n Pr o Se r Cy s G31y As p O31u 40 Ser O31u As p G31y Ser Thr Val O31u As p 55 Cys Ser I Ie Ser O31n Ala Ser Thr Ser 70 O31y O31n Pr o Val As p Lys Ala Leu O31u N/t I Ie Val Ser Ar g 31y (31n Phe Val 100 105 Val I Ie Leu 10 Pro Phe Asp Leu Thr Leu Val Ser Tyr Val l'b t Ala Lys Leu Thr 90 31y Asp (1u Page 814 As p A a Ar g O31u O31u O31u As p Hi s O31u ValI A a O31u Ph e Le u 110 Tyr ValI ValI I Ie O31u lie seqi i st car r ect ed. t xt Asp G31y Val Al a Phe Ser G31y Val Al a Lys Tyr Pro Al a Ar g Val Lys 115 120 125 Cys Ala Leu Leu O31y Tr p Lys Al a Ph e O31n Al a Al a Thr Ala Asp Ala 130 135 140 Val Ala Hi s Al a Hi s 145 <210> 661 <211> 1167 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> ODS <222> (1144) <223> RXA01747 <400> 661 cgaagt agag ccgat tgcag aat cggcgga at gagacgt c gaaaagcgt t t aagct t tcc ct aaaaat at cact aact cg aaagat gt aa ggt tgcatt t g aa O31u at t I Ie gag O31u gt g ValI cgt Ar g g at As p gag O31u t cc Ser t gg Tr p ac c Thr 1 50 cga Ar g ac g Thr c ag O31n c ag O31n gca Al a t gc Cys ct g Le u at c I Ie 120 t ac Tyr gt g ValI ct g Le u cct Pr o at g vb t gct Al a tt c Ph e at c I Ie gt c ValI ggt G31y g aa O31u aac As n ct a Le u cga Ar g aag Lys5 G31y ct c Le u 75 aac As n gct Al a gt t ValI gt g ValI ct g Le u 155 cgc Ar g t gg Tr p g aa O31u t gt Cys at t I Ie t cg Ser gag O31u ac c Thr gt t ValI 140 gt g ValI g aa O31u ag a Ar g gt c ValI aat As n 99c G31y cgc Ar g gt t ValI 110 g at As p aag Lys5 g at As p gct Al a 15 aac As n gct Al a at c I Ie gcc Al a cct Pr o 95 cgt Ar g g at As p at c I Ie tt c Ph e cga Ar g c ag O31n O31y cat Hi s aac As n 80 gag O31u gag O31u ct g Le u c ac Hi s t cc Ser 1 60 gt g ValI 1 aac As n gt c ValI gca Al a gag O31u t gc Cys cca Pr o at g vb t g at As p gag O31u 145 ggc (31y act at c gca Th r I Ie Al a t ca Ser aaa Lys5 t ca Ser t gt Cys t ct Ser ct c Le u c ag O31n g at As p 130 ct g Le u aag Lys5 g aa O31u aac As n ct a Le u t gg Tr p cgc Ar g g ac As p ct g Le u 115 g aa O31u aac As n aag Lys5 ac c Thr gga O31y c ac Hi s g aa O31u cgc Ar g cgc Ar g 1 00 aat As n ggc O31y cca Pr o g at As p cct Pr a ccg Pr a cct Pr a act Thr t cc Ser t gt Cys ggt O31y t ac Tyr gca Al a c ac Hi s ct g Le u 1 Page 815 seqi i st car r ect ed. t xt ct g cag gaa gt t t tt gaa Leu O n O u Val Phe O u t cc cgc cca Ser Ar g Pr o gag gtt 0 u Val t tc gct cac aac gt g Phe A a Hi s Asn Val g aa G31u gag O31u ac c Thr ac c Thr 230 at c I Ie t gg Tr p at g vb t cgt Ar g at c I Ie 310 gcc A a ac c Thr gt g ValI t ca Ser 200 t cc Ser gcg A a c ag O31n aag Ly s tt c Ph e 280 ggc G31y gca A a g aa O31u gt g ValI 1 70 cgt Ar g g at As p ct g Le u c ag O31n ct g Le u 250 gag (31u gct A a ct g Le u ct c Le u t ct Ser 330 at t I Ie gt g ValI at t I Ie g at As p 235 cgt Ar g gag G u gt t ValI t ac Tyr gcg A a 315 aca Thr tt c Ph e at c I Ie tt g Le u 220 ct g Le u cct Pr o tt c Ph e at g vb t gcg A a 300 c ac Hi s ct t Le u cgc Ar g 1 90 c ag O31n at g vb t g ac As p cct Pr o gag (31u 270 ggc O31y gcc A a aag Ly s gag (31u cgc cca Ar g Pr o cgc gat Ar g As p gaa acc O3 u Thr 225 ggc t gt 31y cys 240 ttc cac Phe Hi s gct gat A a As p t tg gt t Leu Val gag tt c O3 u Phe 305 act t cc Thr Ser 320 t ac ggt Ty r 03 y gca A a tt c Ph e 210 aag Ly s g ac As p ccc Pr o gct A a cgt Ar g 290 cgt Ar g gga O31y gct A a tt c Ph e 1 95 ggt O31y g aa O31u at c I Ie at c I Ie gca A a 275 t cc Ser ggc O31y gga O31y t cc Ser 1 cgc Ar g ct g Le u g aa O31u at c I Ie gag O31u 260 aag Ly s t ct Ser gag O31u t cc Ser g aa O31u 340 t ac Tyr gt g ValI at c I Ie ac c Thr 245 cgt Ar g g aa G u t ac Tyr g aa O3u ac c Thr 325 g ac As p 643 691 739 787 835 883 931 979 1 027 1 075 1123 1167 t cc t tc aac Ser Phe Asn taagcccgaa gttttttaac cgc <210O> 662 <21 1> 348 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 662 Val Thr lieAla Pro Olu Oy Arg Arg 1 5 Asn Ser 31u Th r Pr o IIe O31u Th r Lys 25 Val Lys Asn O31y Pro O31u Tyr O31n As p 40 Ala Se r Leu Hi s Thr Val Cys O31n O31u 55 O31u Cy s Tr p O31u Ser Ar g O31u Ala Thr Leu Leu Arg Val Ou Ala Arg 10 Pr o Ar g Tr p I Ie Ar g As n O31n l'b t Lys O31u Ar g Val Ala O31y Ala O31y Cy s Pr o As n I Ie Hi s Phe Leu I I e 03 y 03 y A a As n Page 816 seqI i st car r ect ed. t xt CYS Pr o vb t As p (31u 145 G31y Ph e Pr o As p Thr 225 cys Hi s As p ValI Ph e 305 Ser G31y Ar g As p Le u 115 (31u As n Ly s Hi s Ph e 1 95 G31y Ou le le Al a 275 Ser G31y G31y Ser cys G31y Tyr Al a Hi s Le u 1 65 ValI Tyr ValI I Ie Thr 245 Ar g (31u Tyr (31u Thr 325 As p Asp Phe Cys [Vbt G31u Ser Tr p Thr 150 Le u (31u (31u Thr Thr 230 l 1e Tr p vb t Ar g SI e 310 Al a Thr Pr o Thr Le u 135 G31y O31n Thr Ar g Ly s 215 (31u Thr ValI G31y Al a 295 Pr o O31n Pr o Le u I Ie 120 Tyr ValI O31u ValI Ser 200 Ser Al a O31n Ly s Ph e 280 G31y Al a (31u ValI Ar g 1 05 Thr Ser (31u ValI Pr o 1 85 Le u As n Le u Tyr Pr o 265 Al a Ar g Hi s Al a ValI 345 Asn Ser Ala Arg Pro Ala G31u Ser Val Ar g 110 Val Thr Ar g As p As p 125 Val Val Ar g Lys I Ie 140 Leu Val Pro Asp Phe 1 55 O31u Ser Ar g Pr o O31u 1 Ilie Phe Lys Ar g Ilie 1 Val Ilie Arg O31n Al a 205 Ilie Leu G31y 'b t G31y 220 Asp Leu Hi s Asp Al a 235 Ar g Pr o G31y Pr o Leu 255 O3 u Phe Leu 03 u Hi s 270 Val I'b t Ser G31y Pr o 285 Tyr Al a 03 n Al a I'b t 300 Al a Hi s Leu Lys Asp 315 Thr Leu Leu O31u Ar g 335 Phe Asn O31u G31u Le u Hi s Ser 1 ValI Ar g Ar g O31u G31y 240 Ph e Al a Le u O31u Th r 320 Ty r <210O> 663 <21 1> 876 <212> DNA <213> Cor ynebact er i urn gI ut anm cumn <220> <221 OIDS <222> (853) <223> RXA01746 <400> 663 aaaccgccac t cccct t t ca ct ggggagt g gcggt t t t gt cgt t t cat gc at gcagt gt g t gact tat ca acct tgt tag ggct agggt g gat at ctat c at g act gca cca aga 115 Page 817 seql i st cor r ect ed. t xt lVbt Thr Al a Pro Ar g gat Asp at t SIle t at Tyr cag G n acc Thr gat Asp t at Tyr aga Ar g gcc Al a cat Hi s 150 act Thr ttg Leu ttg Leu tta Leu ct g Leu 230 ttt Phe at t SIle gca Al a ctt Leu ccg Pr o ggt Gy at c SIle ct c Leu 120 gct Al a ggt Sy ggt Sy ttc Phe aca Thr 200 gag G u gtt Val ccc Pr o cgg Ar g ctt Leu ttg Leu gat Asp cgc Ar g aaa Lys gaa G u cgc Ar g gt g Val gca Al a 170 gag G u tcg Ser tcg Ser gat Asp gca Al a tt g Leu gct Al a gag G u ctt Leu 75 at c SIle tta Leu gcg Al a at t SIle gac Asp 155 at g bet cac Hi s agg Ar g at c SIle cat Hi s 235 gat Asp ggt Gy acc Thr cac Hi s ccc Pr o acg Thr gcc Al a ct c Leu gat Asp 140 agc Ser cac Hi s at c SIle gaa G u cgc Ar g 220 tct Ser ctt Leu ttg Leu cgt Ar g 45 ccg Pr o acc Thr tgg Tr p gat Asp at c SIle 125 ggg G y aag Lys ggt G y at t SIle ct g Leu 205 gca Al a ttc Phe tct Ser at c I e agg Ar g tcg Ser aac Asn cat Hi s ccg Pr o 110 caa G n cgt Ar g gtt Val gt g Val ccg Pr o 190 aaa Lys ttg Leu ggc Gy at c I e 15 gat Asp gct Al a gt g Val gga Gy ggt Gy 95 at c SIle gtt Val tcg Ser gcg Al a gcc Al a 175 tgt Cys agg Ar g gat Asp agc Ser cgc Ar g t at Tyr aat Asn t at Tyr ct g Leu 80 cct Pr o gat Asp gt c Val ggt G y gcc Al a 160 at c l Ie ggc Gy gac Asp gat Asp gcg Al a 240 gcg Al a caa G n gat Asp acc Thr ccg Pr o ggc Gy gt g Val ggc G y gt g Val 145 at c l Ie aac Asn at t SIle gtt Val gct Al a 225 ccc Pr o tct Ser gag G u gaa G u gca Al a gt g Val caa G n gtt Val gat Asp 130 tgg Tr p ggc G y tgc Cys gct Al a t ca Ser 210 ttg Leu gac Asp gca Al a gcc Al a at c SIle ggt G y at c SIle ttg Leu gat Asp 115 at g Met gt g Val at t SIle aac Asn gat Asp 195 gtt Val gct Al a cca Pr o gag G u tgg Tr p cct Pr o aag Lys aat Asn gt g Val 100 t ac Tyr ggt G y cca Pr o cga Ar g aac Asn 180 gca Al a gag G u ggt G y act Thr ccc Pr o gat Asp gat Asp cgc Ar g gct Al a at c SIe gta Val gtt Val gct Al a at a SIe 165 acg Thr ggc G y gaa G u cgg Ar g aag Lys 245 aat ctc cct aaa Asn Leu Pro Lys cgg ggg Arg G y 250 tagtacgagg aattttgtcg gtg Page 818 seql i st cor r ect ed. t xt <210> 664 <211> 251 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 664 let Thr Al a 1 Al a G n Asp Thr Pr o Gy Val Gy Val 145 I le Asn SIle Val Al a 225 Pr o Al a Al a I le Gy SIle Leu Asp 115 Met Val SIle Asn Asp 195 Val Al a Pr o Pro Arg 5 G u Pro Trp Asp Pro Asp Lys Ar g Asn Ala Val lile 100 Tyr Val G y Val Pr o Al a Arg Ile 165 Asn Thr 180 Al a G y G u G u G y Arg Thr Lys 245 SIle Ty r G n Thr 70 Asp Ty r Ar g Al a Hi s 150 Thr Leu Leu Leu Leu 230 Asn G u G n Leu G n Ar g Pr o Ar g Gy 135 Asp Ar g Asp Ser Val 215 Val Leu Asp Pro Phe Phe SIle Al a 40 Leu Pr o Gy SIle Leu 120 Al a Gy Gy Phe Thr 200 G u Val Pr o G n G u SIle G u Gy SIle 105 G u Gy Tr p Val Tyr 185 Leu Pr o Ser Lys Pr o 10 Ar g Leu Leu Asp Ar g 90 Lys G u Ar g Val Al a 170 G u Ser Ser Asp Ar g 250 Al a Leu Al a G u Leu I le Leu Al a SIle Asp 155 let Hi s Ar g SIle Hi s 235 Gy Asp Gy Thr Hi s Pr o Thr Al a Leu Asp 140 Ser Hi s SIle G u Ar g 220 Ser Leu Leu Ar g Pr o Thr Tr p Asp SIle 125 Gy Lys Gy I e Leu 205 Al a Phe I e Asp Al a Val Gy Gy I e Val Ser Al a Al a 175 Cys Ar g Asp Ser <210> 665 <211> 1179 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1156) <223> RXA02106 <400> 665 ggtcgccgct tttcgacgcc cgcctgcggc gggatgactg tgatggaggg gcgcgtcgac Page 819 seql i st cor r ect aagcaaaatc tctttagcaa attcggttac tgtggggcgc ed. txt gag G u gat Asp gaa G u tcg Ser ttg Leu at t SIle gat Asp aac Asn cgt Ar g at c SIle 150 aat Asn at g Met gaa G u gat Asp t ac Tyr 230 gct Al a aaa Lys gat Asp gat Asp ggt Gy gaa G u tta Leu gaa G u gcg Al a 120 ccg Pr o agt Ser cat Hi s at g Met ct c Leu 200 tgg Tr p cct Pr o cag G n cct Pr o at t SIle gca Al a aac Asn gat Asp gt g Val gaa G u gat Asp at g Met cag G n gt g Val 170 ggc Gy gct Al a at t SIle aat Asn cgt Ar g 250 ggt Gy gct Al a tt c Phe act Thr gac Asp 75 cgt Ar g at c SIle gag G u cgc Ar g t ca Ser 155 acc Thr ggc Gy ggt Gy gct Al a gac Asp 235 cgc Ar g gga Gy gac Asp ttc Phe gat Asp gtt Val cgg Ar g ct g Leu ct c Leu at t SIle 140 t at Tyr gt g Val aac Asn ttg Leu gcg Al a 220 at c SIle agt Ser aag Lys gt g Val gcc Al a 45 cgt Ar g gag G u gca Al a cac Hi s ctt Leu 125 tgg Tr p gt c Val gt a Val tgc Cys agc Ser 205 ct g Leu acc Thr ggc Gy ctt Leu aag Lys ctt Leu ttg Leu ct a Leu gt c Val cca Pr o 110 ttg Leu gat Asp aat Asn cga Ar g at c SIle 190 t at Tyr aag Lys t cc Ser gca Al a gt c Val t cc Ser cgg Ar g gca Al a ggc Gy 80 ggc Gy gt g Val caa G n gat Asp at c SIle 160 at g Met t at Tyr cag G n cac Hi s ggc Gy 240 ct c Leu at g Met 1 gtt Val ggc Gy gcg Al a aag Lys ttt Phe gcg Al a ctt Leu gtt Val gat Asp 145 aac Asn tct Ser t cc Ser t cc Ser gat Asp 225 gga Gy cac Hi s gat Asp gat Asp ct g Leu ttg Leu agc Ser caa G n cct Pr o gcc Al a 130 cgc Ar g caa G n ggt Gy ct g Leu t at Tyr 210 gtt Val aaa Lys cac Hi s gt g Val ttc Phe cag G n gat Asp act Thr gat Asp acc Thr 115 agt Ser gcc Al a gaa G u ggc Gy t at Tyr 195 gaa G u gac Asp at c SIle gt g Val acc Thr ttc Phe ggg Gy gca Al a gcg Al a ttc Phe 100 cca Pr o ggt Gy aca Thr ggc Gy ggt Gy 180 gca Al a t at Tyr gct Al a ggt Gy acc Thr 260 acc Thr ct c Leu gcg Al a gcg Al a gat Asp acc Thr ctt Leu cag G n gtg Val gtt Val 165 gca Al a ccg Pr o ttg Leu tgg Tr p ggc Gy 245 at g let aat aac cat ttt Asn Asn His Phe Page 820 t cc tat gac Ser Tyr Asp aag gtg aag Lys Val Lys 280 gat cct ct g Asp Pro Leu 295 acc cta aag Thr Leu Lys 310 ct c agc gat Leu Ser Asp tac gcc acc Tyr Al a Thr aag <210> 666 <211> 352 <212> PRT gat Asp t cc Ser cgc Ar g aca Thr gat Asp 330 gag G u gcg Al a gac Asp caa G n tt c Phe 315 tt c Phe tgg Tr p gac Asp aag Lys aca Thr 300 agt Ser gcg Al a act Thr seql i st cor r ect ed. t xt atg atg acc cag gtg ttg cgc att gga 931 'e t bet Thr G n Val Leu Arg lie y 270 275 ggt ctt cgc agc gca aag aag cgc gtt 979 G y Leu Arg Ser Al a Lys Lys Arg Val 285 290 ggt gca tca cgt gag caa atc atc gac 1027 G y Al a Ser Arg G u n Ile Ile Asp 305 gct agg tac ggc gcg caa gaa gta gag 1075 Ala Arg Tyr y Ala n u Val u 320 325 gca ggc cac gac cta gta aaa acc aaa 1123 Ala G y His Asp Leu Val Lys Thr Lys 335 340 aag cga gtt caa tagtttctat ggatctgcac 1176 Lys Arg Val G n 350 1179 <213> Corynebacter i um gl ut ai cum <400> 666 IVet Asn Asn 1 Val Gy Al a Lys Phe Al a Leu Val Asp 145 Asn Ser Val Phe G n Asp Thr Asp Thr 115 Ser Al a G u Gy Hi s Phe G u 5 Thr Thr Asp Phe Leu G u G y Al a Ser Al a Al a Leu 70 Al a Asp I e Phe Thr Asp 100 Pro Leu Asn G y G n Ar g Thr Val I le 150 Sy Val Asn 165 G v Al a Mbet Leu Leu Pr o Val Al a Al a Tyr Val Gy 135 Gy G u Phe Val Ser G u Asp Tyr Al a Tr p 105 Leu Thr Phe Gy G u 185 Pr o 10 SIle Al a Asn Asp Val 90 G u Asp Met G n Val 170 Gy Al a Phe Thr Asp Ar g SIle G u Ar g Ser 155 Thr Lys Val Al a Ar g G u Al a Hi s Leu 125 Tr p Val Val Val I e Gy G n Hi s Thr Gy Asp Tr p G u Ar g 175 Sy y Asn Cys Ile Thr Tyr 190 Page 821 Ser Ser Asp 225 Gy Hi s Val Al a G u 305 Al a Leu Tyr 195 G u Asp SIle Val Ar g 275 Lys SIle G u Lys Pr o Leu Tr p Gy 245 Met Gy Val Asp G u 325 Lys Ser Ar g 215 Val Al a Tyr Val Pr o 295 Leu Ser Al a seql i st cor r ect ed. t xt Leu Val Ala y Leu Ser 200 205 Trp Val Ile Ala Ala Leu 220 Pro Ile Asn Asp Ile Thr 235 G n Lys Arg Arg Ser y 250 Asp Ile Asp Ala Asp IVet 265 Lys lie Ser Asp Lys G y 280 285 Leu Arg Arg G n Thr y 300 Lys Ser Thr Phe Ser Al a 315 Asp G u Asp Phe Al a Al a 330 Thr G u G u Trp Thr Lys 345 Tyr Lys Ser Al a Met 270 Leu Al a Ar g Gy Ar g 350 G u Thr Thr Val 255 Thr Ar g Ser Tyr Hi s 335 Val <210> 667 <211> 403 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(403) <223> RXS01183 <400> 667 cttgatatga cccgaacacc acacatcaca aattgaatcg ttccgtttta acgacacgac ttgcgaggag tcttaaaata gag G u tgg Tr p gag G u ggt Gy ccc Pr o aag Lys t ca Ser at c SIle ct g Leu gtt Val gac Asp gag G u gaa G u gac Asp gt c Val aag Lys t ca Ser act Thr gac Asp 45 gct Al a gt a Val gtt Val acc Thr gaa G u acc gaa Thr G u 15 gag gta G u Val gag att G u Ile gag gat Su Asp Page 822 gtatcctttg gggtattagt atg gcg ttc tcc gta IVet Ala Phe Ser Val 1 ggc acg atc acc cag Sy Thr Ile Thr n gat gag ccg ttg ctc Asp G u Pro Leu Leu ccc tct cct gtc gcc Pro Ser Pro Val Ala gac acc gtc gac gtc Asp Thr Val Asp Val seqI i st car r ect ed. t xt ggc ggt gt c at t gca at a at c ggc gat gct gat gag act cct gcc aac G31yOy Val IleAla Ilie IleOy AspAlaAspOu Thr Pro Al aAs n 75 80 gaa gct cct gcc gac gag gca cca gct cct gcc gaa gag gaa gaa cca O3 u Ala Pro Ala Asp 0 u Ala Pro Ala Pro Al a 03 u 03 u 03 u 03 u Pr o 95 100 <210O> 668 <21 1> 101 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 668 I'b t Al a Phe Ser Val G31u I'bt Pro (31u 1 5 G31y Th r I Ie Thr O31n Tr p Leu Lys Ser 25 Asp G31u Pro Leu Leu G31u Val Ser Thr 40 Pr o Ser Pr o Val Al a G31y Val Ilie Leu 55 Asp Thr Val Asp Val G31y G31y Val Ilie 70 03 u Thr Pr o Al a Asn 03 u Al a Pr o Al a G31u G31u 31u G31u Pr o 1 00 Le u 10 ValI As p G31u Al a As p 90 G31y G31y Ly s I Ie I Ie 75 (31u G31u As p ValI Ly s lie Al a Ser Thr As p Al a G31y Pr o ValI ValI Thr G31u As p Al a Thr G31u G31u G31u Al a Pr o G31u ValI I Ie As p As p Al a <210O> 669 <21 1> 1305 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (1282) <223> RXS01260 <400> 669 ct aaacgt gg gct gcat t cc t t ccaaagt c t ct gat caaa ct t tacccat gagaagaaga cct tcggcat caat ggcgaa gag G31u G31y ct t Le u g at As p gct Al a cat Hi s aac As n ggc (31y aag Ly s tt g Le u aag Ly s ac c Thr t cc Ser aag Ly s gct Al a ac c Thr ggc G31y aac As n act Thr g at As p gt t t cc Val Ser 15 aag at c Lys I I e ct t gag Leu 03 u gac t gc As p Cys Page 82,: aacgct gaag t t gcccat ac gt g acc t tc aac t at Val Thr Phe Asn Ty r 1 gac aag at c gt t gga As p Lys I Ie Val G31y at c gaa at t cat ggt I e 03 u I I e Hi s 03 y gt c acc gac ggt aag Val Thr Asp 03 y Lys at c at c gca acc ggt Il e Il e Al a Thr 03 y seql i st cor r ect ed. t xt 60 tcg gta gtc aac acc ctc cgt ggc gtt gac ttc tca gag aac gtt gtg 355 Ser Val Val Asn Thr Leu Arg Gy Val Asp Phe Ser G u Asn Val Val 75 80 tct ttt gaa gag cag att ctt aac cct gtt gcg cca aag aag atg gtc 403 Ser Phe G u G u G n Ile Leu Asn Pro Val Ala Pro Lys Lys Mbt Val 95 100 att gtt ggt gca ggc gca att gga atg gaa ttc gcc tac gtt ctt ggt 451 Ile Val y Al a y Ala Ile y lVbt u Phe Ala Tyr Val Leu y 105 110 115 aac tac ggt gta gat gta acc gtc atc gag ttc atg gat cgt gtg ctt 499 Asn Tyr y Val Asp Val Thr Val Ile G u Phe IVet Asp Arg Val Leu 120 125 130 cca aat gaa gat gct gaa gtc tcc aag gtt att gca aag gcc tac aag 547 Pro Asn G u Asp Ala G u Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 135 140 145 aag at g ggc gtt aag ctt ctt cct ggc cat gca acc act gct gtt cgg 595 Lys IVbt G y Val Lys Leu Leu Pro G y His Ala Thr Thr Ala Val Arg 150 155 160 165 gac aac ggt gac ttt gtc gag gtt gat tac cag aag aag ggc tct gac 643 Asp Asn y Asp Phe Val G u Val Asp Tyr G n Lys Lys y Ser Asp 170 175 180 aag aca gag act ctt act gtt gat cga gtc atg gtt tcc gtt ggt ttc 691 Lys Thr G u Thr Leu Thr Val Asp Arg Val IVbt Val Ser Val G y Phe 185 190 195 cgt cca cgc gtt gag gga ttt ggt ctt gaa aac act ggc gtt aag ctc 739 Arg Pro Arg Val G u G y Phe y Leu G u Asn Thr y Val Lys Leu 200 205 210 acc gag cgt ggc gca atc gag atc gat gat tac atg cgt acc aac gtc 787 Thr u Arg Gy Ala lie u lie Asp Asp Tyr IVbt Arg Thr Asn Val 215 220 225 gat ggc att tac gcc atc ggt gac gtg acc gcc aag ctt cag ctt gct 835 Asp Gy Ile Tyr Ala lie y Asp Val Thr Ala Lys Leu n Leu Ala 230 235 240 245 cac gtc gca gaa gca cag ggc att gtt gcc gca gag act att gct ggt 883 His Val Ala G u Al a Gn G y IIe Val Ala Al a u Thr Ile Ala G y 250 255 260 gca gaa act cag act ctt ggt gat tac atg atg atg cca cgt gca acc 931 Al a G u Thr G n Thr Leu G y Asp Tyr IVbt IVbt IVbt Pro Arg Al a Thr 265 270 275 ttc tgc aac cca cag gtt tct tcc ttt ggt tac acc gaa gag cag gcc 979 Phe Cys Asn Pro G n Val Ser Ser Phe y Tyr Thr G u G u G n Ala 280 285 290 aag gag aag tgg cca gat cgt gag atc aag gtt gct tcc ttc cca ttc 1027 Lys G u Lys Trp Pro Asp Arg G u lie Lys Val Ala Ser Phe Pro Phe 295 300 305 tct gca aac ggt aaa gca gtt ggc ctg gca gaa act gat ggt ttc gca 1075 Ser Ala Asn y Lys Al a Val y Leu Ala G u Thr Asp y Phe Ala 310 315 320 325 aag atc gtt gct gat gca gaa ttc ggt gag ctg ctc ggt gca cac ctg 1123 Lys IIe Val Ala Asp Al a G u Phe G y G u Leu Leu G y Ala His Leu Page 824 seqI i st car r ect ed. t xt 330 335 340 gtt gga gca aat gca tca gag ct cat caat gaat t ggt gct t gct cag Val G31y Al a Asn Al a Ser (31u Leu I Ie Asn (31u Leu Val Leu Al a O31n 345 350 355 aac t gg gat ct c acc act gaa gag at c t ct cgt agc gt c cat at t cac Asn Tr p Asp Leu Thr Thr O31u 31u Ilie Ser Ar g Ser Val Hi s Ilie Hi s 360 365 370 cca acg ct a t ct gag gca gt t aag gaa gct gca cac ggt at c t ct gga Pr o Thr Leu Ser O31u Al a Val Lys O31u Al a Al a Hi s 31y Ilie Ser G31y 375 380 385 cac at g at c aac t tc t agaat ccac ct cgt tggcc ct g His 'b t Ilie Asn Phe 390 <210> 670 <211> 394 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 1171 1219 1267 1305 <400> 670 Val Thr Phe Asn Asp Lys I I e Val Ile 0 u IlIe Hi s Val Thr As p G31y 1 1 e I I e Al a Th r Ser 03 u As n Val Pr o Lys Lys I'b t 1 00 Al a Ty r Val Leu 115 I'b t As p Ar g Val 130 Al a Lys Al a Ty r 145 Thr Thr Al a Val Lys Lys 03 y Ser 1 80 Val Ser Val 03 y 1 95 Thr 03 y Val Lys 210 Tyr G31y G31y Lys5 G31y ValI ValI G31y Le u Lys5 Ar g 1 65 As p Ph e Le u G uAs p 0 y Val Leu 0 y As p Al a 55 Ser Val 70 Ser Phe I e Val Asn Tyr Pr o As n 135 Lys I'b t 150 As p As n Lys Thr Ar g Pr o Thr 0 u 215 Al a Hi s As n 40 G31y ValI 031u G31y G31y 120 031u G31y G31y 031u Ar g 200 Ar g Hi s Tyr Ph e Lys5 As n 031u Al a 1 ValI As p ValI As p Thr 1 ValI (31y Ly s 10 Le u Lys5 Thr Thr O31n 90 G31y As p Al a Lys5 Ph e 1 70 Le u O31u Al a Ar g vb t As p I Ie Le u 75 li1e Al a ValI O31u Le u 1 55 ValI Thr Gy le Ser Ly s Al a Thr Ar g Le u I Ie Thr ValI 140 Le u O31u ValI Ph e O31u 220 Ar g Ly s Ly s Ph e G31y As n G31y ValI 125 Ser Pr o ValI As p G31y 205 li1e ValI Ly s Le u As p As p ValI O31u O31u ValI Hi s Tyr 1 ValI O31u As p Ser I Ie O31u cys Ph e Al a Ph e Ph e I Ie Al a 1 O31n vb t As n Ty r I'bt Arg Thr Asn Val Asp Gy IlieTyr Ala Ilie Gy Asp Val Thr Ala Page 825 seql i st cor r ec 225 230 235 Lys Leu G n Leu Ala His Val Ala u Ala G n 245 250 Su Thr Ile Al a y Al a u Thr n Thr Leu 260 265 IVet Pro Arg Ala Thr Phe Cys Asn Pro G n Val 275 280 Thr G u G u n Ala Lys G u Lys Trp Pro Asp 290 295 Ala Ser Phe Pro Phe Ser Ala Asn y Lys Al a 305 310 315 Thr Asp y Phe Ala Lys Ile Val Ala Asp Al a 325 330 Leu G y Ala His Leu Val G y Ala Asn Ala Ser 340 345 Leu Val Leu Ala G n Asn Trp Asp Leu Thr Thr 355 360 Ser Val His Ile His Pro Thr Leu Ser G u Ala 370 375 His G y IIe Ser G y His IVet Ile Asn Phe 385 390 <210> 671 <211> 294 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(271) <223> RXS01261 <400> 671 gtgggtgttt ttcattttct tccactctaa aattaagtat atgcacgaca atgacccact aaacacgtat ccttgaatgc ted. txt G y lle Sy Asp Ser Ser 285 Arg G u 300 Val G y G u Phe G u Leu G u G u 365 Val Lys 380 Al a 255 Mt Sy Lys Al a G u 335 Asn Ser Al a 240 Al a Mt Tyr Val G u 320 Leu G u Ar g Al a gac gt a gt a Asp Val Val cgt gca gcg Ar g Al a Al a tgg ggt ggt Trp G y y gat caa aaa Asp G n Lys <210> 672 ctc gga gcc ggc ccc ggt ggc Leu y Ala G y Pro G y G y 15 ctt ggc aag aag gtt gct gta Leu G y Lys Lys Val Ala Val tgc cta aac gtg ggc tgc att Cys Leu Asn Val y Cys Ile 45 tgaagt t gcc cataccttta ccc ggaaaaccaa ccgcacccgg gtg act gaa cat tat Val Thr G u His Tyr 1 tat gtc tcc gcc atc Tyr Val Ser Ala lle att gag aag cag tac Ile G u Lys G n Tyr cct tcc aaa gtc tct Pro Ser Lys Val Ser Page 826 seqi i st car r ect ed. t xt <21 1> 57 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 672 ValI Th r G31u Hi s Ty r As p ValI ValI ValI L eu G31y Ala G31y Pr o 31y G31y 1 5 10 Ty r Val Se r Ala I Ie Ar g Ala Ala O31n L eu O31y Lys Lys Val Ala Val 25 li1e O31u Lys O31n Ty r Tr p 31y G31y Val Cys Leu Asn Val G31y Cys I Ie 40 Pr o Ser Lys Val Ser Asp O31n Lys Ar g <210O> 673 <21 1> 1005 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (982) <223> RXA02717 <400> 673 aggcat gt cc ct aacgaaca t cccagcct c at ct caat gg gcaat tagcg acgtt t tgaa gcgt cct tca cccggccgag t acct tt t tc t gt cgagtt t at g cca ccc cgc l'bt Pro Pro Arg g at As p ct c Le u cgt Ar g ac c Thr aag Ly s gcg A a ac c Thr t cc Ser gct A a gca A a ag a Ar g ct g Le u at t I Ie cga Ar g gga G31y cct Pr a 120 gag O31u tt t Ph e t ca Ser c ac Hi s cgg Ar g g at As p ct g Le u tt c Ph e ct t Le u t cc Ser at t I Ie ac c Thr t ac Tyr cct Pr a t at Tyr g aa O31u t ac Tyr gt g ValI gct A a 45 ct g Le u ct a Le u gga G31y gcc A a tt c Ph e 125 cgc Ar g act Thr cga Ar g gt t ValI gag O31u g ac As p t ct Ser 110 g ac As p gca A a 15 t at Tyr cga Ar g aac As n ct g Le u cca Pr a 95 gag O31u ct c Le u gca A a ggt G31y Le u c ac Hi s gga G31y tt a Le u ct c Le u ggt G31y 1 gag O31u gct A a gcg A a act Thr tt a Le u ggc G31y at c I Ie at c I Ie gt c ValI ggc G31y aaa Ly s cgc Ar g ac a Thr g at As p g at As p gcc A a 130 tt c Ph e gga G31y c aa O31n g aa O31u aac As n t gg Tr p ct t Le u 115 t cc Ser cat Hi s t ca Ser ccg Pr a gag O31u ct g Le u gt g ValI 1 00 at t I Ie tt c Ph e gaa ggg cat ttc cgg gcg aaa act cta gaa gaa gac acc aaa tac act Page 827 seql i st cor r ect ed. t xt G u y His Phe Arg Ala Lys Thr Leu G u G u Asp Thr Lys Tyr Thr 135 140 145 ctg gcg aag ctg cgt gga ggg gca gag tac tcc atc acg cag atg ttc 595 Leu Ala Lys Leu Arg G y Gy Ala G u Tyr Ser Ile Thr G n IVet Phe 150 155 160 165 ttt gat gtg gaa gac tac ctg cga ctt cgt gat cgc ctt gtc gct gca 643 Phe Asp Val G u Asp Tyr Leu Arg Leu Arg Asp Arg Leu Val Ala Ala 170 175 180 gac ccc att cat ggt gcg aag cca atc att cct ggc atc atg ccc att 691 Asp Pro li e His G y Ala Lys Pro li e li e Pro G y I I e It Pro li e 185 190 195 acg agc ctg cgg tct gtg cgt cga cag gtc gaa ctc tct ggt gct caa 739 Thr Ser Leu Arg Ser Val Arg Arg G n Val G u Leu Ser y Ala G n 200 205 210 ttg ccg agc caa cta gaa gaa tca ctt gtt cga gct gca aac ggc aat 787 Leu Pro Ser G n Leu u u G uSer Leu Val Arg Ala Ala Asn y Asn 215 220 225 gaa gaa gcg aac aaa gac gag atc cgc aag gtg ggc att gaa tat tcc 835 Su u Ala Asn Lys Asp u lie Arg Lys Val y Ile u Tyr Ser 230 235 240 245 acc aat atg gca gag cga ctc att gcc gaa ggt gcg gaa gat ctg cac 883 Thr Asn IVet Ala u Arg Leu lie Ala u Gy Ala u Asp Leu His 250 255 260 ttc atg acg ctt aac ttc acc cgt gca acc caa gaa gtg ttg tac aac 931 Phe IVet Thr Leu Asn Phe Thr Arg Ala Thr G n u Val Leu Tyr Asn 265 270 275 ctt ggc atg gcg cct gct tgg gga gca gag cac ggc caa gac gcg gtg 979 Leu G y Iet Al a Pro Al a Trp G y Al a u His G y n Asp Al a Val 280 285 290 cgt taagccctct taggaatcat gaa 1005 Ar g <210> 674 <211> 294 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 674 IVet Pro Pro Arg Asp Asp Ala Ala u u Arg Leu Tyr Arg Ala Ala 1 5 10 G u Val Phe His Asp Leu G y Ala Ser Phe Val Ser Val Thr Tyr G y 25 Al a y G y Ser Thr Arg G u Arg Thr Ser Arg lie Al a Arg Arg Leu 40 Ala Lys G n Pro Leu Thr Thr Leu Val His Leu Thr Leu Val Asn His 55 Thr Arg u u IVet Lys Ala lie Leu Arg u Tyr Leu u Leu y 70 75 Leu Thr Asn Leu Leu Ala Leu Arg y Asp Pro Pro y Asp Pro Leu 90 Page 828 seqI i st car r ect ed. t xt 31y As pTr pVa I Se rTh rAs p(1y (1y L eu As nTy rA 100 105 a Ser 03 u Leu 110 le le As p 145 li1e Ar g G31y Le u A a 225 G31y A a O31u G31y Le u 115 Ser Ly s O31n ValI vb t 1 95 G31y As n O31u As p Le u 275 As p Ly s Pr o Thr Ph e 1 65 A a I Ie O31n As n Ser 245 Hi s As n ValI Thr G31y 135 A a As p Pr o Ser Pr o 215 O31u As n vb t G31y Pr o 120 Hi s Ly s ValI I Ie Le u 200 Ser A a vb t Thr vb t 280 O31u Ph e Le u O31u Hi s 1 85 Ar g O31n As n A a Le u 265 A a Ar g A a G31y 1 Tyr A a ValI (31u As p 235 Ar g Ph e A a Ph e 125 Thr A a Ar g Pr a Ar g 205 Ser le le Ar g G31y 285 As p Le u (31u Le u I Ie 1 O31n Le u Ar g A a A a 270 A a Le u O31u Tyr Ar g 1 li1e ValI ValI Ly s O31u 255 Thr (31u <210O> 675 <21 1> 601 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (601) <223> RXN02027 <400> 675 t cacgt gct c at cgat aggc aaact cat gg aaccaagagt t cgt cgt acc cct ccgaaaa t aacggt tat cct tagatt t cag gaa ct g 03 n 03 u Leu gct t ct cgc Ala Ser Ar g t ac at c cgc Tyr Ilie Ar g cgc acg aag ctt cgg gaa Ar g Thr Lys Leu Ar g O31u acg cga gaa aac gca gcc Thr Ar g 03 u As n A a A a 30 tca aag cag cca aaa agg Ser Lys (31n Pr o Lys Ar g 45 gcg cgc Ala Ar g 15 at c at t I Ie I Ie at t gcc I e A a Page M2 acct gccccc gcaaccccaa atg agc caa act aag I'b t Ser 03 n Th r Lys 1 acc aat at g gat gag Thr Asn I'b t Asp 03 u gcc aac gt t t ct t at A a Asn Val Ser Tyr gct t ac gt g ccg gt g A a Tyr Val Pr o Val seql i st cor r ect ed. t xt cgt acc gaa cct ggt ggg cga ttg ctt ctt gac gcc ctc cac gcc gaa 307 Arg Thr G u Pro G y G y Arg Leu Leu Leu Asp Ala Leu His Ala G u 60 act tcc gcg ctt att ttg cca gtc tcc ctc gag gat cga cgc ctc gac 355 Thr Ser Ala Leu Ile Leu Pro Val Ser Leu G u Asp Arg Arg Leu Asp 75 80 tgg gct ctt tat gaa ggc cca acc agc ctt gtt cct ggc gca ttt ggc 403 Trp Ala Leu Tyr G u G y Pro Thr Ser Leu Val Pro G y Ala Phe G y 95 100 atc cag gaa ccc ggt ggc act cgc ctt gga cct gaa gct ctc aac ttc 451 lie On Gu Pro Gy Gy Thr Arg Leu G y Pro G u Ala Leu Asn Phe 105 110 115 tgc gac ctt gtc atc gcc ccc gca ctg gca tgc acc ccc agt gga atc 499 Cys Asp Leu Val Ile Al a Pro Ala Leu Ala Cys Thr Pro Ser Gy Ile 120 125 130 agg cta ggt aaa ggt ggc ggt ttc tac gac cgc gct cta gcc acc gga 547 Arg Leu Gy Lys Gy Gy y Phe Tyr Asp Arg Ala Leu Ala Thr y 135 140 145 gtc aag gct gac gtg att act ttg ctc ttc aac gga gaa atc cgc gac 595 Val Lys Ala Asp Val I e Thr Leu Leu Phe Asn Gy G u lie Arg Asp 150 155 160 165 gat atc 601 Asp I e <210> 676 <211> 167 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 676 IVet Ser G n Thr Lys n G u Leu Arg Thr Lys Leu Arg G u Ala Arg 1 5 10 Thr Asn IVet Asp G u Al a Ser Arg Thr Arg u Asn Ala Ala lie lie 25 Ala Asn Val Ser Tyr Tyr lie Arg Ser Lys n Pro Lys Arg lie Ala 40 Ala Tyr Val Pro Val Arg Thr u Pro Gy Gy Arg Leu Leu Leu Asp 55 Ala Leu His Ala G u Thr Ser Ala Leu Ile Leu Pro Val Ser Leu G u 70 75 Asp Arg Arg Leu Asp Trp Ala Leu Tyr G u y Pro Thr Ser Leu Val 90 Pro y Ala Phe y Ile n u Pro y y Thr Arg Leu y Pro 100 105 110 G u Ala Leu Asn Phe Cys Asp Leu Val Ile Ala Pro Ala Leu Ala Cys 115 120 125 Thr Pro Ser y lie Arg Leu y Lys y y y Phe Tyr Asp Arg 130 135 140 Ala Leu Ala Thr y Val Lys Ala Asp Val lie Thr Leu Leu Phe Asn Page 830 seql i st cor r ect ed. t xt 155 145 150 Gy Ou lie Arg Asp Asp Ile 165 <210> <211> <212> <213> <220> <221> <222> <223> 677 595 DNA Cor ynebact eri um gl ut anm cum CDS (595) FRXA02027 <400> 677 tcacgtgctc atcgataggc aaactcatgg aaccaagagt acctgccccc gcaaccccaa tcgtcgtacc cctccgaaaa taacggttat ccttagattt agc caa act Ser G n Thr cag gaa ctg cgc Sn G u Leu Arg acg Thr aag ctt cgg gaa Lys Leu Arg G u gcg Al a 15 cgc acc aat atg Arg Thr Asn IVet gat gag Asp u gct tct cgc Ala Ser Ar g tac atc cgc Tyr li e Ar g cga gaa aac gca Arg G u Asn Ala atc att gcc aac lie Ile Ala Asn gtt tct tat Val Ser Tyr gtg ccg gtg Val Pro Val tca aag cag cca Ser Lys G n Pro aaa Lys agg att gcc gct Arg Ile Ala Ala cgt acc Arg Thr gaa cct ggt ggg G u Pro G y G y cga Ar g 60 ttg ctt ctt gac Leu Leu Leu Asp gcc Al a ctc cac gcc gaa Leu His Ala G u act Thr tcc gcg ctt att Ser Ala Leu Il e cca gtc t cc ct c Pro Val Ser Leu gag G u gat cga cgc ctc Asp Arg Arg Leu tgg gct ctt tat Trp Ala Leu Tyr gaa Su ggc cca acc agc G y Pro Thr Ser gtt cct ggc gca Val Pro G y Ala ttt ggc Phe G y 100 atc cag gaa lie Gn u ccc ggt ggc act cgc ctt gga cct gaa gct ctc aac ttc Pro Gy G y Thr Arg Leu y Pro G u Ala Leu Asn Phe 105 110 115 tgc gac ctt gtc atc gcc ccc gca ctg gca tgc acc ccc agt gga atc Cys Asp Leu Val Ile Ala Pro Ala Leu Ala Cys Thr Pro Ser Gy lie 120 125 130 agg ct a Arg Leu 135 ggt aaa ggt ggc G y Lys G y G y gct gac gtg att Ala Asp Val li e 155 ggt G1y 140 ttc tac gac cgc Phe Tyr Asp Arg gct Al a 145 cta gcc acc gga Leu Al a Thr G y gt c Val 150 aag Lys act ttg ctc ttc Thr Leu Leu Phe aac Asn 160 gga gaa atc cgc Gy u lie Arg <210> 678 <211> 165 <212> PRT <213> Corynebacteri um gl utam cum Page 831 <400> 678 l'bt Ser G3 n Thr As n Wt Al a As n Val Al a Ty r Val Al a Leu Hi s As p Ar g Ar g Pro G31y Ala (3 u A a Leu 115 Thr Pr o Ser 130 A a Leu A a 145 G31y O31u I Ie Ly s G31u Tyr ValI O31u As p G31y Ph e I Ie G31y As p 1 O31n A a Tyr Ar g Thr 70 Tr p SI e Cy s Ar g ValI 150 O31u Ser I Ie Thr 55 Ser A a O31n As p Le u 135 Ly s seql i st car r ect ed. t xt Leu Arg Thr Lys Leu Arg 10 Ar g Thr Arg G31u As n Ala Ar g Ser Lys O31n Pr o Lys 40 O31u Pr o G31y G31y Ar g Leu Ala Leu I Ie Leu Pr o Val 75 Leu Ty r 31u G31y Pr o Thr 90 O31u Pr o G31y G31y Thr Ar g 1 05 Leu Val I Ie Ala Pr o Ala 120 125 03 y Lys 03 y 03 y 03 y Phe 140 Ala As p Val I Ie Thr Leu 1 55 O31u A a Ar g Le u Ser Ser Le u 110 Le u Ty r Le u A a lie lIe Le u Le u Le u G31y A a As p Ph e Ar g I Ie A a As p O31u ValI Pr o cys Ar g As n 1 <210O> 679 <21 1> 579 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (556) <223> RXA00106 <400> 679 t ggagct caa caaggcagcg t acat gt t tg agt acagct t gct acgat cc acacccat tg at ccgcggca aggt cgccgt t gg Tr p t gg Tr p c ag O31n ccg Pr o c aa O31n at c I Ie gt c ValI CCC Pr o cgt Ar g g aa O31u at g vb t cgc Ar g ggC O31y ct c Le u cgt Ar g aac As n at c I Ie aaa Ly s cgc Ar g 45 tt c Ph e at c I Ie c ac Hi s ac g Thr at t I Ie ggc gac 31y As p 15 ttc aag Phe Lys t gg gag Tr p O31u ct c t cc Leu Ser Page 83,, cgat gacat c accgt gt ccg at g at c ggt gcg at t l'b t I Ie O31y Ala I Ie 1 ggc acc gac at g ccc O31y Thr Asp l'b t Pr o aaa acc acc atg ggc Lys Thr Thr l'b t 03 y t ct t tg ccg t tc aag Ser Leu Pro Phe Lys t ca cgc gag ccc ggc Ser Ar g (3u Pr o (3y seqI i st car r ect ed. t xt gac tgg tcc gcc ggc ggc aca gtg gtc acc gaa atc cct Asp Tr p Ser Al a 31y G31y Thr Val Val Thr O31u I Ie Pro 75 80 tgg atc atg ggc ggc ggc gag gtc tac aag gcc acc gtc Tr p IIe 'b t G31y G31y 31y (31u Val Tyr Lys Al a Thr Val 95 gac gt t t ta gaa at a acg ct t at c gac gcc acc t tc gat Asp Val Leu (31u Ilie Thr Leu Ilie Asp Al a Thr Phe Asp 105 110 ccc gtc tac gca ccc gaa atc ccg gcg aac ttc aac ctc Pr o Val Tyr Al a Pr o 31u Ilie Pr o Al a Asn Phe Asn Leu 120 125 130 t cc gag tgg t tt acc tca ggc gag tat cgt t ac aag tt c Ser (31u Tr p Phe Thr Ser G31y (31u Tyr Ar g Tyr Lys Phe 135 140 145 atc aag gtt taaggagcaa acaacatgag caa Ile Lys Val 1 <210> 680 <211> 152 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn aaa Ly s ggc G31y gt t ValI 115 g at As p c ag 0 n ag c Ser ag c Ser 1 00 t cc Ser g ac As p cgc Ar g ggc G31y gcc Al a act Thr g aa G31u t ac Tyr <400> 680 I'b t I Ie 03y Al a IlieTrp Al a 0n 0 y G31y Ly s Ser Ser li1e Thr Ph e As n Ly s 145 As p Thr Pr o G31u Ly s G31y ValI 115 As p O31n Pr o G31y Ly s G31y G31y Al a Thr O31u Tyr Hi s Pr o Le u Tr p I Ie ValI ValI O31u 135 Ly s Pr o li1e G31y Al a G31y O31u 1 05 Al a Ph e As p As p G31y O31u G31y 75 G31y Thr O31u Ser I Ie Ly s Ar g Ph e ValI ValI I Ie Pr a 125 (31u I Ie Hi s Thr I Ie ValI Tyr As p 110 Al a Tyr G31y Ph e Tr p Le u Thr Ly s Al a As n Ar g As p Ly s O31u Ser O31u Al a Thr Ph e Ty r <210O> 681 <21 1> 1044 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn Page 833 seql i st cor r ect ed. t xt <220> <221> CDS <222> (101)..(1021) <223> RXN01321 <400> 681 ggagggtgta gtcaaggatt tgggccatgg tggagcggga acttagacct gacttagtgt gggaaaattt ccagggtaga aat cgt cat a t ccat accct ccg Pr o cgt Ar g gcg Al a gct Al a gcg Al a gag G u ttc Phe gag G u t at Tyr t at Tyr 150 aag Lys gt g Val at t SIle at t SIle tct Ser ttt Phe ttg Leu t at Tyr cgc Ar g gct Al a gac Asp cac Hi s 120 at g Met gcg Al a gcg Al a ggt Gy ccg Pr o 200 cac Hi s gaa G u ct c Leu tcg Ser acg Thr gag G u ctt Leu gcg Al a ttg Leu gtt Val aac Asn ggt Gy 170 gat Asp gat Asp ttc Phe cgt Ar g ttt Phe ct a Leu cct Pr o at t SIle gag G u gt g Val gat Asp gcg Al a 140 aat Asn cgg Ar g gat Asp tgt Cys ccg Pr o 220 aat Asn ggc Gy gct Al a 45 gat Asp gat Asp gag G u aag Lys ttg Leu 125 gtt Val gtt Val aag Lys gcg Al a gag G u 205 tcg Ser cgt Ar g tgc Cys gag G u tcg Ser acc Thr ttc Phe aag Lys 110 tta Leu gt g Val ccg Pr o gcg Al a at t l Ie 190 at g Met tt t ttt Phe agc Ser gac Asp ggg Gy tgg Tr p at t I e ccg Pr o gt g Val cgt Ar g aac Asn ttc Phe 160 gac Asp ttg Leu gct Al a ggt Gy at g let 1 gct Al a t cc Ser ggt Gy ttc Phe gag G u agg Ar g ttg Leu gt g Val cat Hi s 145 cat Hi s cag G n gct Al a ggt Gy gcg Al a 225 gcg Al a act Thr tgg Tr p ttt Phe cag G n gct Al a ttg Leu gct Al a 130 gag G u gt g Val gt c Val cgt Ar g cgt Ar g 210 cgc Ar g gaa G u att SIe act Thr cgt Ar g cgg Ar g tgg Tr p 100 tct Ser aat Asn ttg Leu ttt Phe gag G u 180 at g Met ttg Leu t at Tyr caa cga atg Sn Arg lIet Page 834 seql i st cor r ect ed. t xt cag gcg tat agc cgt ggt gtg aag ttg att ggt gcg acc G n Ala Tyr Ser Arg G y Val Lys Leu lie y Ala Thr 230 235 240 gcg act ggg gat ctg gat gat ggt ccg atc att gag cag Ala Thr y Asp Leu Asp Asp y Pro lie lie 3 u n 250 255 cgt gtg acg cat aag gat acg ccg act gag atg cag cgt Arg Val Thr His Lys Asp Thr Pro Thr G u Vet G n Arg 265 270 gat gcg gag aag cag gtg ctg gct cgc ggt ttg cgt ttc Asp Ala G u Lys G n Val Leu Ala Arg y Leu Arg Phe 280 285 290 gac cgg gtg ctg gtt tac ggt aac cgc acg gtt gtc ttt Asp Arg Val Leu Val Tyr G y Asn Arg Thr Val Val Phe 295 300 305 taaggctttt tgcttttcga cgc <210> 682 <211> 307 <212> PRT <213> Corynebacter i um gl ut ai cum cat tat Hi s Tyr 245 gtt att Val I e 260 ggc cgc G y Arg ttg gag Leu G u 1021 1044 <400> 682 Met 1 Al a Ser Gy Phe G u Ar g Leu Val Hi s 145 Hi s G n Al a Sn Arg IVet Ala Pro G u Thr G y li e Trp Il e Thr Phe Thr Ar g G n Leu Ar g Ala Lys Trp 100 Leu Val Ser 115 Al a u Asn 130 G u Asn Leu Val Pro Phe Val Ala u 180 Arg Phe IVet 195 Thr Pro Ser Ser Pro G u 5 10 G u Arg G n Phe Val Leu 25 Val Al a Lys Leu Ser Ser 40 G u Al a y Tyr Phe Thr Sn Ala lie Arg Ala u 70 G u G u Phe Ala Pro Leu 90 Ser Phe Thr Asp Thr Ala 105 Lys G u G y Hi s Cys Leu 120 Asp Tyr Pro Iet u Val 135 Arg Tyr Ile Ala u Asn 150 Pro Lys Asp Ala Val y 165 170 lie Val Asn y Tyr Asp 185 G n I e Leu Pro Pro Asp 200 Val Thr Phe Asp Ser Al a G n Hi s Val Hi s 155 Lys Pr o Asn Sy Al a Asp Asp G u Lys Leu 125 Val Val Lys Al a Ar g Cys G u Ser Thr Phe Lys 110 Leu Val Pr o Al a l Ie 190 Pr o Pr o Ar g Asn Thr Sy Al a Sy Gy Phe Phe 175 Val Leu Cys G u Iet Trp Ala 205 Page 835 seql i st cor r ect ed. t xt G y Arg Val Leu Asn I e His His Ser Phe Leu Pro Ser Phe Met G y 210 215 220 Ala Arg Pro Tyr His G n Ala Tyr Ser Arg G y Val Lys Leu I I e G y 225 230 235 240 Ala Thr Cys His Tyr Ala Thr G y Asp Leu Asp Asp G y Pro lie lie 245 250 255 G u G n Asp Val Ile Arg Val Thr His Lys Asp Thr Pro Thr G u IVet 260 265 270 G n Arg Leu y Arg Asp Ala G u Lys G n Val Leu Ala Arg y Leu 275 280 285 Arg Phe His Leu G u Asp Arg Val Leu Val Tyr y Asn Arg Thr Val 290 295 300 Val Phe Asp 305 <210> 683 <211> 582 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (23)..(559) <223> FRXA01321 <400> 683 cttgcacgat ttgttaggtc gt gtg gct gag aat gat tat ccg atg gaa gtt 52 Val Ala u Asn Asp Tyr Pro IVet u Val 1 5 gtt gcg gtt gtg ggt aac cat gag aac ttg cgt tat att gcg gag aac 100 Val Ala Val Val G y Asn His G u Asn Leu Arg Tyr lie Ala G u Asn 20 cat aat gtt ccg ttt ttc cat gtg ccg ttt cct aag gat gcg gtt ggt 148 His Asn Val Pro Phe Phe His Val Pro Phe Pro Lys Asp Ala Val y 35 aag cgg aag gcg ttt gac cag gtc gct gag att gtg aat ggt tat gat 196 Lys Arg Lys Ala Phe Asp G n Val Ala G u Ile Val Asn y Tyr Asp 50 ccg gat gcg att gtt ttg gct cgt ttt atg cag att ttg ccg ccg gat 244 Pro Asp Ala lie Val Leu Ala Arg Phe IVet n Ile Leu Pro Pro Asp 65 ttg tgt gag atg tgg gct ggt cgt gtg ttg aat att cat cac agt ttc 292 Leu Cys G u IVet Trp Al a y Arg Val Leu Asn lie His His Ser Phe 80 85 ttg ccg tcg ttt atg ggt gcg cgc ccg tat cat cag gcg tat agc cgt 340 Leu Pro Ser Phe Iet G y Ala Arg Pro Tyr His G n Ala Tyr Ser Arg 100 105 ggt gtg aag ttg att ggt gcg acc tgc cat tat gcg act ggg gat ctg 388 Sy Val Lys Leu lie G y Ala Thr Cys His Tyr Ala Thr y Asp Leu 110 115 120 gat gat ggt ccg atc att gag cag gat gtt att cgt gtg acg cat aag 436 Asp Asp y Pro lie lie G u n Asp Val Ile Arg Val Thr His Lys 125 130 135 Page 836 seql i st cor r ect ed. t xt gat acg ccg act gag atg cag cgt ttg ggc cgc gat gcg gag aag cag Asp Thr Pro Thr G u Vet G n Arg Leu y Arg Asp Ala G u Lys n 140 145 150 gtg ctg gct cgc ggt ttg cgt ttc cac ttg gag gac cgg gtg ctg gtt Val Leu Ala Arg G y Leu Arg Phe His Leu G u Asp Arg Val Leu Val 155 160 165 170 tac ggt aac cgc acg gtt gtc ttt gat taaggctttt tgcttttcga Tyr Gy Asn Arg Thr Val Val Phe Asp 175 cgc <210> 684 <211> 179 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 684 Val Ala Gu 1 Hi s Hi s G n Al a Gy Al a Al a G u G n 145 Ar g Val Asn Pr o Al a Phe Val Pr o Cys 115 Asp Leu Hi s Asp Asn Asp Tyr 5 Leu Arg Tyr Phe Pro Lys G u Ile Val e t n lie 70 Leu Asn II e Tyr His G n 100 Hi s Tyr Al a Val IIe Ar g G y Arg Asp 150 Leu G u Asp 165 Pr o SIle Asp Asn Leu Hi s Al a Thr Val 135 Al a Ar g Met Al a Al a 40 Sy Pr o Hi s Tyr Sy 120 Thr G u Val Su Val 10 Gu Asn 25 Val G y Tyr Asp Pro Asp Ser Phe 90 Ser Arg 105 Asp Leu Hi s Lys Lys G n Leu Val 170 Val Ala Val Val Gy Asn Hi s Lys Pr o Leu Leu Sy Asp Asp Val 155 Tyr Val Lys Al a G u Ser Lys Sy 125 Pr o Al a Asn Pr o Al a l Ie Met Phe Leu 110 Pr o Thr Ar g Ar g Phe Phe Val Tr p bet I e SIe G u Sy Thr 175 <210> 685 <211> 975 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(952) <223> RXA00461 Page 837 seql i st cor r ect ed. t xt <400> 685 tgttgggagg gatgacagga ttgtcgaaga taacgtgaag tgggtgttcc ggcatgtgtt tgattgtaag gccttggaaa agggtggaat aatagcgggc gtg act gca atc aaa Val Thr Ala lle Lys ctt Leu cgc Ar g gt g Val cat Hi s cct Pr o aac Asn aag Lys gat Asp gag G u cgt Ar g 150 cgt Ar g t cc Ser gct Al a ccg Pr o gat Asp 230 gga Gy gct Al a gt g Val gac Asp gat Asp gat Asp ttg Leu gat Asp 120 gct Al a ttc Phe gt c Val aac Asn gag G u 200 at g let ggc Gy tta Leu ttg Leu gat Asp gag G u acg Thr tct Ser gaa G u ct g Leu ct g Leu gtt Val gtt Val 170 acg Thr cgt Ar g acc Thr t cc Ser tac Ty r aag Lys gac Asp cag G n cag G n 75 tgc Cys aac Asn cac Hi s cca Pr o gag G u 155 ggt Gy gtt Val gcg Al a gca Al a cgc Ar g 235 cgc Ar g gag Gu cca Pr o at t SIle gaa Gu act Thr gct Al a cct Pr o tgc Cys 140 ctt Leu cgc Ar g act Thr gct Al a gac Asp 220 aag Lys ggg Gy aaa Lys gcg Al a 45 ggt Gy gag Gu ggt Gy gt g Val gt a Val 125 acc Thr gat Asp cca Pr o ttg Leu gac Asp 205 at g let gac Asp gaa Gu ggg Gy agc Ser gt g Val ctt Leu t ac Tyr ct g Leu 110 aac Asn ccg Pr o ggc Gy at t SIle tgc Cys 190 gt c Val gt c Val ggc Gy ttc Phe gt g Val tct Ser tcg Ser gct Al a gt g Val cgc Ar g ggc Gy ggt Gy aag Lys 160 ct g Leu act Thr at t SIle cca Pr o ttg Leu 240 1 gcc Al a ccg Pr o t ac Tyr at c SIle gt c Val cag G n at t SIle aag Lys t cc Ser 145 gtt Val at g Met ggc Gy gct Al a ggc Gy 225 ctt Leu gac Asp ggg Gy gt g Val cgt Ar g at c SIle ctt Leu gat Asp ctt Leu 130 at c SIle gtt Val ct g Leu acg Thr gca Al a 210 gca Al a ggc Gy ttg Leu ctt Leu aag Lys aag Lys gat Asp cct Pr o cca Pr o 115 gt g Val agc Ser gt c Val acc Thr aag Lys 195 gct Al a gcg Al a gac Asp gaa G u gcc Al a at g Met gat Asp gaa Gu ttg Leu 100 gct Al a ct c Leu ttg Leu att SIe cgc Ar g 180 gat Asp ggt Gy gtg Val gtc Val Page 838 ccc gac gtg Pro Asp Val gtt ggc cct Val G y Pro gct gaa aag Al a G u Lys 280 gaa G u 250 acc Thr gct Al a gt c Val cgt Ar g gga Gy gcc Al a gca Al a ct c Leu seql i st cor r ect ed. t xt ggc gcg gtc tca cca aac cca ggc ggc Sy Ala Val Ser Pro Asn Pro Gy y 255 260 ttc ttg gtg cac aat gtt gtc gag cgc Phe Leu Val His Asn Val Val G u Arg 270 275 t aaaaacaca t gact aat cc cgg <210> 686 <211> 284 <212> PRT <213> Corynebacteri um gl utam cum <400> 686 Val 1 Al a Pr o Tyr SIle Val G n SIle Lys Ser 145 Val Met Gy Al a Gy 225 Leu Thr Ala li e Asp Leu G u G y Leu Al a Val Lys Met Arg Lys Asp Ile Asp G u Leu Pro Leu 100 Asp Pro Al a 115 Leu Val Leu 130 Ile Ser Leu Val Val I le Leu Thr Arg 180 Thr Lys Asp 195 Al a Al a G y 210 Al a Al a Val G y Asp Val Lys 5 G n Thr Lys Leu Leu Pr o Lys Asn Leu Gy 165 Ar g Leu G n Leu Hi s 245 Leu Asp Arg Val Val Leu His Ar g 55 Pr o Al a 70 Asn Asn Lys Hi s Asp Al a G u Pro 135 Arg Arg 150 Arg G y Ser G u Al a Al a Pro Hi s 215 Asp Val 230 Pro Asp Gy Al a Val 40 Asp Asp Asp Leu Asp 120 Al a Phe Val Asn G u 200 bet Gy Val Asn Al a 25 Gy Cys Val Asp Asp 105 Gy Pr o Gy Thr Ser 185 Thr Leu Val Tr p Tyr Lys Asp G n G n Cys Asn Hi s Pr o G u 155 Gy Val Al a Al a Ar g 235 Val Ar g G u Pr o I e G u Thr Al a Pr o Cys 140 Leu Ar g Thr Al a Asp 220 Lys Al a Gy Lys Al a Gy G u Gy Val Val 125 Thr Asp Pr o Leu Asp 205 bet Asp Gy G u Gy Ser Val Leu Tyr Leu 110 Asn Pr o Gy SIle Cys 190 Val Val Gy Al a I e I e Hi s Asn Phe SIe G u Leu Asn Al a Gy 175 Hi s SIe Lys Lys Val 255 Pro Asn Pro Gy Gy Val Gy Pro Leu Thr Arg Ala Phe Leu Val His Page 839 seql i st cor r ect ed. txt 260 265 Asn Val Val G u Arg Ala G u Lys Leu Ala y Leu 275 280 <210> 687 <211> 711 <212> DNA <213> Corynebacteri um gl utam cum <220> <221> CDS <222> (101)..(688) <223> RXA01514 <400> 687 accacagaaa tgcctgtcgt tccagatcag cccatcgat g gagggcacac aggagaatcc ggaaaatgaa ggagacaacc gtgattccgg gaagtccgct gca Al a ttg Leu acc Thr cat Hi s gaa G u cac Hi s ggt Gy gat Asp at t SIle gt g Val 150 cct Pr o cgc Ar g at c SIle gct Al a gat Asp ttg Leu ttg Leu t cc Ser ttt Phe 120 gat Asp gaa G u gct Al a ttc Phe gt g Val gt g Val acc Thr ct g Leu ccg Pr o aag Lys aag Lys ct c Leu gag G u acc Thr 170 gag G u gag G u agg Ar g gt g Val cgt Ar g ttt Phe act Thr cct Pr o gaa G u 140 ct g Leu acg Thr cgc Ar g cca Pr o t ac Tyr gag G u at c lile gt g Val ct g Leu 110 gtt Val ctt Leu at g let gcg Al a gca Al a 15 gat Asp aag Lys aag Lys ccg Pr o gcg Al a 95 t cc Ser cag G n gat Asp gcc Al a gt g Val 175 aca Thr cgc Ar g gaa Gu acg Thr at t SIle cac Hi s aag Lys gag Gu gcc Al a at g let 160 cgc Ar g gt g Val 1 gct Al a gaa Gu act Thr ttc Phe t ac Tyr at t SIle ct g Leu cgc Ar g cag G n 145 cgc Ar g ggc Gy gcg Al a G y ttc Phe tct Ser t cc Ser ggt G y gcg Al a ttg Leu 130 gcc Al a gga gty ggt Gy cgt Ar g ttg Leu ggt G y ggc G y tgt Cys att SIe 100 tta Leu t cc Ser gcc Al a cgt Ar g aag Lys 180 gat aac cac Asp Asn Hi s aac gct gcc tcc cgc gct gag gtg ttc tcc ctg att cgg ggg cac Page 840 seql i st cor r ect ed. t xt Asn Ala Ala Ser Arg Al a u Val Phe Ser Leu IIe Arg Gy His 185 190 195 taaatgaacg tatcctcttt gac <210> 688 <211> 196 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 688 Val Asp Asn His Ala Ala 1 Al a G u Thr Phe Tyr SIle Leu Ar g G n 145 Ar g Gy lie l Ie Leu Al a G u Met Tyr Ar g 115 Thr Val SIle Phe Gy 195 5 G u G u Leu Hi s G u Pr o Al a G n Val Lys 165 Asn Val Leu Pr o G u G u Hi s Lys Mbet Al a 135 I le Gy Al a Ar g SIle Al a 40 Asp Leu Leu Ser Phe 120 Asp G u Al a Al a G u Al a Ar g Pr o Val Val Gy 105 Al a Al a Al a Val Ser 185 Asp Gy Al a Thr Val 75 Phe Val Ar g Val Hi s 155 Thr Al a G u Asp Al a Leu G u Gy Gy G n 125 Lys Cys Ser Val Ar g Pr o Tyr G u SIle Val Leu 110 Val Leu Met Al a Phe 190 Al a Asp Lys Lys Pr o Al a Ser G n Asp Al a Val 175 Ser Thr Ar g G u Thr SIe Hi s Lys G u Al a Met 160 Ar g Leu <210> 689 <211> 513 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(490) <223> RXA01516 <400> 689 tctgcacata tgggagcatg gggtgtgcgc gtgcacgatg tcccagtatc aagggacgct gttgatgttg ccgcattgtg gcgaagtgga ggaactcacc atg gct gat cgt att 115 Iet Ala Asp Arg Ile 1 Page 841 seql i st cor r ect ed. t xt gaa ctt aaa ggc G u Leu Lys G y gaa aaa gag caa G u Lys G u G n gat ttc gat gcc Asp Phe Asp Al a tac ggc gcg ttg Tyr G y Ala Leu agg gat ttg atc Arg Asp Leu Ile gct aaa ttt gat Ala Lys Phe Asp aaa gca ccg at c Lys Ala Pro Ile 105 cgt tcc agg aaa Arg Ser Arg Lys 120 ctt gaa tgc ttc gga Leu G u Cys Phe G y ggc cag ccc ttc att Sy n Pro Phe lie 30 gca ggt gcc agc gat Al a y Ala Ser Asp 45 gca ttg ttg gtt gct Ala Leu Leu Val Ala 60 gag acg gtg gcc acg G u Thr Val Ala Thr 75 gcg ctt cat gcg gt g Ala Leu His Ala Val cca cgt act ttt gct Pro Arg Thr Phe Ala 110 t cc atg gct gct gga Ser Net Al a Al a G y 125 cac cac ggt Hi s Hi s G y 15 gtg gat gtc Val Asp Val gac ctt tcc Asp Leu Ser gaa at c gt g G u I Ie Val gaa t ct gcg G u Ser Al a 80 gaa gta acc G u Val Thr 95 gac gtc gcg Asp Val Al a agg agc aac Arg Ser Asn gt g Val acc Thr gac Asp gaa G u gat Asp at c SIle gt g Val gcc Al a 130 ttc gac ttt Phe Asp Phe tgc tgg atg Cys Trp IVet acc gt a gat Thr Val Asp ggc cca t cc G y Pro Ser gct gtg atg Al a Val Met cat aag ccc His Lys Pro 100 gtt gcc cga Val Al a Arg 115 t aat gcat gc agttttgtcc atc <210> 690 <211> 130 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 690 IVet Ala Asp Arg lie u Leu Lys y 1 5 Sy Val Phe Asp Phe G u Lys G u G n 25 Val Thr Cys Trp IVet Asp Phe Asp Ala 40 Ser Asp Thr Val Asp Tyr y Ala Leu 55 Val G u y Pro Ser Arg Asp Leu Ile Ala Asp Ala Val IVet Ala Lys Phe Asp Thr Ile His Lys Pro Lys Ala Pro Ile 100 105 Ala Val Val Ala Arg Arg Ser Arg Lys 115 120 Asn Ala 130 Leu 10 Sy Al a Al a G u Al a 90 Pr o Ser Cys Pr o Al a Leu Val Hi s Thr Al a Phe Phe Ser Val Al a Al a Phe Al a 125 Gy I e Asp Al a Thr Val Al a 110 Sy Hi s Val Asp G u G u G u Asp Ar g Page 842