CN1370234A - 编码参与内环境稳定和适应的蛋白质的谷氨酸棒杆菌基因 - Google Patents
编码参与内环境稳定和适应的蛋白质的谷氨酸棒杆菌基因 Download PDFInfo
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Abstract
本发明描述了命名为HA核酸分子的分离的核酸分子,它编码谷氨酸棒杆菌的新型HA蛋白。本发明也提供反义核酸分子、含有HA核酸分子的重组表达载体和已经导入所述表达载体的宿主细胞。本发明还提供分离的HA蛋白、突变型HA蛋白、融合蛋白、抗原性肽和用于基于谷氨酸棒杆菌中HA基因的基因工程而改进谷氨酸棒杆菌的所需化合物生产的方法。
Description
相关申请
本申请要求于1999年6月25日申请的美国临时专利申请顺序号60/141031的优先权。本申请也要求以下专利申请的优先权:1999年7月8日申请的德国专利申请号19931636.8、1999年7月9日申请的德国专利申请号19932125.6、1999年7月9日申请的德国专利申请号19932126.4、1999年7月9日申请的德国专利申请号19932127.2、1999年7月9日申请的德国专利申请号19932128.0、1999年7月9日申请的德国专利申请号19932129.9、1999年7月9日申请的德国专利申请号19932226.0、1999年7月14日申请的德国专利申请号19932920.6、1999年7月14日申请的德国专利申请号19932922.2、1999年7月14日申请的德国专利申请号19932924.9、1999年7月14日申请的德国专利申请号19932928.1、1999年7月14日申请的德国专利申请号19932930.3、1999年7月14日申请的德国专利申请号19932933.8、1999年7月14日申请的德国专利申请号19932935.4、1999年7月14日申请的德国专利申请号19932973.7、1999年7月14日申请的德国专利申请号19933002.6、1999年7月14日申请的德国专利申请号19933003.4、1999年7月14日申请的德国专利申请号19933005.0、1999年7月14日申请的德国专利申请号19933006.9、1999年8月31日申请的德国专利申请号19941378.9、1999年8月31日申请的德国专利申请号19941379.7、1999年8月31日申请的德国专利申请号19941390.8、1999年8月31日申请的德国专利申请号19941391.6和1999年9月3日申请的德国专利申请号19942088.2。所有上述申请的全部内容均通过引用结合到本文中。
发明背景
在细胞中天然发生的代谢过程的某些产物和副产物在多种多样的工业中具有实用性,所述工业包括食品工业、饲料工业、化妆品工业和制药业。这些分子统称为“精细化学品”,包括有机酸、生成蛋白质的(proteinogenic)和非生成蛋白质的氨基酸、核苷酸和核苷、脂质和脂肪酸、二元醇、糖类、芳族化合物、维生素和辅因子和酶。它们的生产最为方便的是通过开发用以生产和分泌大量的一种或多种所需分子的细菌的大规模培养来进行。用于此目的的一种特别有用的生物是谷氨酸棒杆菌(Corynebacterium glutamicum),这是一种革兰氏阳性的非致病菌。通过菌株的选择,已经开发了许多产生一系列所需化合物突变型菌株。然而,在特定分子生产方面改进的菌株的选择是一个费时且困难的过程。
发明概要
本发明提供具有多种用途的新型细菌核酸分子。这些用途包括可以用来生产精细化学品的微生物的鉴定、谷氨酸棒杆菌或相关细菌中精细化学品生产的调节、谷氨酸棒杆菌或相关细菌的定型或鉴定、作为谷氨酸棒杆菌基因组作图的参比点以及作为转化的标记。这些新型核酸分子编码本文中称为稳态和适应(homeostasis and adaptation)(HA)蛋白的蛋白质。
谷氨酸棒杆菌是一种革兰氏阳性需氧菌,通常用于工业中以大规模生产种类繁多的精细化学品,也用于糖类降解(例如用于石油溢出)以及用于萜类化合物的氧化。因此,本发明的HA核酸分子可以用来鉴定可以用以例如通过发酵过程生产精细化学品的微生物。本发明HA核酸表达的调节或本发明HA核酸分子序列的修饰,可以用以调节得自微生物的一种或多种精细化学品的生产(例如以改进得自棒杆菌或短杆菌菌种的一种或多种精细化学品的收率或产量)。
本发明的HA核酸也可以用来将生物鉴定为谷氨酸棒杆菌或其密切相关菌种,或用来鉴定混合微生物群体中谷氨酸棒杆菌或其相关菌种的存在。本发明提供多种谷氨酸棒杆菌基因的核酸序列;通过用跨越谷氨酸棒杆菌基因区的探针,在严格条件下探测特有微生物或混合微生物群体的培养物的提取的基因组DNA,人们可以确定该生物是否存在。虽然谷氨酸棒杆菌本身是非致病性的,但它与人类中的致病菌种例如白喉棒杆菌(Corynebacterium diphtheriae)(白喉的病原体)相关;这类生物的检测具有重大的临床意义。
本发明的HA核酸分子也可以用作谷氨酸棒杆菌基因组或相关生物基因组作图的参比点。同样,这些分子或其变异体或其部分可以用作基因工程棒杆菌或短杆菌菌种的标记。
由本发明新型核酸分子编码的HA蛋白能够例如执行参与维持谷氨酸棒杆菌内稳态或参与该微生物适应不同环境条件的能力的功能。已知可得到用于谷氨酸棒杆菌的克隆载体例如Sinskey等的美国专利号4,649,119中的克隆载体以及用于谷氨酸棒杆菌和相关短杆菌菌种(例如乳发酵短杆菌(Brevibacterium lactofermentum))遗传操作技术(Yoshihama等,J.Bacteriol.162:591-597(1985);Katsumata等,J.Bacteriol.159:306-311(1984);和Santamaria等,J.Gen.Microbiol.130:2237-2246(1984)),因此本发明的核酸分子可以用于这种生物的基因工程,以使其成为一种或多种精细化学品的更好或更有效的生产者。精细化学品的这种改进的生产或产率可能是由于本发明基因的操作的直接效应引起的,或者它可能是由这类操作的间接效应引起的。
有许多机制使得改变本发明HA蛋白可以直接影响掺入这种改变的蛋白的谷氨酸棒杆菌菌株生产精细化学品的收率、产量或产率。例如,通过对修饰或降解芳族化合物或脂族化合物的酶进行工程改造,使得这些酶在活性或数量上增加或减少,有可能对作为这些化合物的修饰或降解产物的一种或多种精细化学品的产生进行调节。同样,参与所述无机化合物代谢的酶可为诸如氨基酸、维生素和核酸之类的这类精细化学品的生物合成提供关键分子(例如磷、硫和氮分子)。通过改变谷氨酸棒杆菌中这些酶的活性或数量,有可能增加这些无机化合物的转化(或使用替代无机化合物),由此使得这些精细化学品中无机原子掺入的速率提高。对参与普遍性细胞过程的谷氨酸棒杆菌酶进行基因工程,也可以直接改进精细化学品的产生,因为这些酶中的许多酶直接修饰精细化学品(例如氨基酸)或参与精细化学品合成或分泌的酶。对细胞蛋白酶活性或数量的调节也可能对精细化学品的产生具有直接影响,因为许多蛋白酶可以降解精细化学品或参与精细化学品产生或分解的酶。
此外,参与芳族/脂族化合物修饰或降解、一般性生物催化、无机化合物代谢或蛋白水解的上述酶本身均是精细化学品,在各种体外工业应用中需要其活性。通过改变谷氨酸棒杆菌中一种或多种这些酶的基因的拷贝数,有可能增加细胞产生的这些蛋白质的数量,由此提高大规模谷氨酸棒杆菌或相关细菌培养物生产这些蛋白质的潜在收率或产率。
本发明HA蛋白的改变也可能间接影响掺入这类经改变蛋白的谷氨酸棒杆菌菌株生产精细化学品的收率、产量和/或产率。例如,通过对参与细胞壁构建或重排的那些蛋白质的活性和/或数量进行调节,有可能修饰细胞壁本身的结构,致使细胞能够更好地经受住大规模发酵培养期间存在的机械应力和其它应力。另外,谷氨酸棒杆菌的大规模生长需要大量的细胞壁产生。对细胞壁生物合成酶或降解酶的活性或数量进行调节,可以使得细胞壁生物合成的速率更加快速,这进而可以使得培养物中该微生物的生长速率提高,由此增加生产所需精细化学品的细胞数量。
通过修饰本发明HA酶,也可以间接影响谷氨酸棒杆菌生产一种或多种精细化学品的收率、产量或产率。例如,谷氨酸棒杆菌中许多通用酶对通用(global)细胞过程(例如调节过程)具有重大影响,这进而对精细化学品代谢具有重大影响。同样,蛋白酶、修饰或降解可能的毒性芳族或脂族化合物的酶以及促进无机化合物代谢的酶均可用来提高谷氨酸棒杆菌的生存力。这些蛋白酶有助于选择性地除去错折叠或经错误调节的蛋白质,例如在大规模发酵罐培养期间可能遇到的相对胁迫的环境条件下存在的那些蛋白质。通过改变这些蛋白质,有可能进一步增强这种活性以及提高培养物中谷氨酸棒杆菌的生存力。芳族/脂族修饰或降解蛋白质不仅可以用来将这些废物化合物(这些化合物可能作为培养基中的杂质或细胞自身的废物而遇到)解毒,而且允许细胞在培养物中最适碳源有限时可利用替代碳源。通过增加其数量和/或活性,可以提高培养物中谷氨酸棒杆菌细胞的存活。本发明的无机代谢蛋白质为细胞供应所有蛋白质和核苷酸(这只是其中之一)合成所需的无机分子,因此对于细胞的总生存力是至关重要的。增加大规模培养物中生产一种或多种所需精细化学品的活细胞的数量,应该导致同时提高所述培养物中所述精细化学品生产的收率、产量和/或产率。
本发明提供编码本文称为HA蛋白的新型核酸分子,所述HA蛋白能够例如执行参与维持谷氨酸棒杆菌内稳态或参与该微生物适应不同环境条件的能力的功能。编码HA蛋白的核酸分子在本文中称为HA核酸分子。在一个优选实施方案中,所述HA蛋白参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种谷氨酸棒杆菌酶活性或蛋白水解活性。这类蛋白的实例包括由表1中所述基因编码的那些蛋白。
因此,本发明的一个方面涉及分离的核酸分子(例如cDNA、DNA或RNA),所述分离的核酸分子包含编码HA蛋白或其生物活性部分的核苷酸序列,还涉及适合作为检测或扩增HA编码核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在特别优选的实施方案中,所述分离的核酸分子包含序列表中奇数SEQ ID NO(例如SEQ IDNO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7…)中所述核苷酸序列之一、或这些核苷酸序列之一的编码区或其互补序列。在其它特别优选的实施方案中,本发明的分离的核酸分子包含这样的核苷酸序列或其部分,所述核苷酸序列与一种序列表奇数SEQ ID NO(例如SEQID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7…)中所述核苷酸序列杂交或有至少约50%、优选至少约60%、更优选至少约70%、80%或90%、甚至更优选至少约95%、96%、97%、98%、99%或更高同源性。在其它优选实施方案中,所述分离的核酸分子编码序列表偶数SEQ ID NO(例如SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQID NO:8…)中所述氨基酸序列之一。本发明优选的HA蛋白也最好具有至少一种本文所述的HA活性。
在另一实施方案中,所述分离的核酸分子编码一种蛋白质或其部分,其中所述蛋白或其部分包括足以与本发明氨基酸序列(例如具有序列表中偶数SEQ ID NO的序列)同源的氨基酸序列,例如足以与本发明的氨基酸序列同源,使得所述蛋白质或其部分保留HA活性。最好是,由所述核酸分子编码的蛋白质或其部分保留参与维持谷氨酸棒杆菌内稳态或执行参与该微生物适应不同环境条件的功能的能力。在一个实施方案中,由所述核酸分子编码的蛋白质与本发明氨基酸序列(例如选自具有序列表中偶数SEQ ID NO的完整的氨基酸序列)的同源性为至少约50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。在另一优选实施方案中,所述蛋白质是与本发明完整氨基酸序列(由序列表中相应的奇数SEQ ID NO(例如SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQID NO:7…)中所示的可读框编码)基本上同源的全长谷氨酸棒杆菌蛋白质。
在另一优选实施方案中,所述分离的核酸分子得自谷氨酸棒杆菌,并且编码一种包括一个生物活性结构域的蛋白质(例如HA融合蛋白),所述生物活性结构域与一种本发明的氨基酸序列(例如序列表中一种偶数SEQ ID NO的序列)有至少约50%或更高的同源性,并且能够参与谷氨酸棒杆菌中的DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌,或具有表1中所述的一种或多种活性,所述分离的核酸分子也包括编码异源多肽或调节区的异源核酸序列。
在另一实施方案中,所述分离的核酸分子至少长15个核苷酸,并且在严格条件下与包含本发明核苷酸序列(例如序列表中奇数SEQ IDNO的序列)的核酸分子杂交。优选所述分离的核酸分子对应于天然存在的核酸分子。更优选所述分离的核酸编码天然存在的谷氨酸棒杆菌HA蛋白或其生物活性部分。
本发明的另一方面涉及含有本发明核酸分子的载体,例如重组表达载体,涉及已经导入这类载体的宿主细胞。在一个实施方案中,这样一种宿主细胞用来通过在合适的培养基中培养所述宿主细胞而生产HA蛋白。然后从所述培养基或所述宿主细胞中分离出所述HA蛋白。
本发明的再一方面涉及其中已经导入HA基因或改变HA基因的遗传改变的微生物。在一个实施方案中,通过导入作为转基因的编码野生型或突变型HA序列的本发明核酸分子,已经改变了所述微生物的基因组。在另一实施方案中,所述微生物基因组内的内源HA基因通过与改变的HA基因同源重组而已经被改变,例如功能性断裂。在另一实施方案中,微生物中内源的或所引入的HA基因通过一个或多个点突变、缺失或倒位而被改变,但仍编码功能性HA蛋白。在再一实施方案中,微生物中HA基因的一个或多个调节区(例如启动子、阻抑蛋白或诱导物)已经被改变(例如通过缺失、截短、倒位或点突变),使得所述HA基因的表达得到调节。在一个优选实施方案,所述微生物属于棒杆菌属或短杆菌属,特别优选谷氨酸棒杆菌。在一个优选实施方案中,所述微生物也用于生产所需化合物,例如氨基酸,特别优选赖氨酸。
另一方面,本发明提供一种鉴定受治疗者体内白喉棒杆菌存在或其活性的方法。该方法包括检测受治疗者体内的一种或多种本发明的核酸序列或氨基酸序列(例如序列表SEQ ID NO 1-440中所述序列),由此检测所述受治疗者体内白喉棒杆菌的存在或其活性。
本发明的又一方面涉及分离的HA蛋白或其部分,例如生物活性部分。在一个优选实施方案中,所述分离HA蛋白或其部分可以参与维持谷氨酸棒杆菌内稳态或可以执行参与该微生物对不同环境条件适应的功能。在另一优选实施方案中,所述分离的HA蛋白或其部分与本发明的氨基酸序列(例如序列表中偶数SEQ ID NO的序列)足够同源,使得所述蛋白质或其部分保留参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能的能力。
本发明也提供HA蛋白的分离制剂。在优选实施方案中,所述HA蛋白包含本发明的氨基酸序列(例如序列表中偶数SEQ ID NO的序列)。在另一优选实施方案中,本发明涉及与本发明的完整氨基酸序列(例如序列表中偶数SEQ ID NO的序列)(由序列表A中相应奇数SEQID NO所述可读框编码)基本上同源的分离的全长蛋白质。在再一实施方案中,所述蛋白质与本发明完整氨基酸序列(例如序列表中偶数SEQID NO的序列)的同源性为至少约50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。在其它实施方案中,所述分离的HA蛋白包含与本发明的一种氨基酸序列(例如序列表中偶数SEQ ID NO的序列)的同源性至少约50%或更高的氨基酸序列,并且能够参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能,或具有表1中所述的一种或多种活性。
或者,所述分离的HA蛋白可以包含这样一种核苷酸序列编码的氨基酸序列,所述核苷酸序列与序列表中一种偶数SEQ ID NO所述的核苷酸序列杂交,例如在严格条件下杂交,或与序列表中一种奇数SEQID NO所示的核苷酸序列的同源性至少约50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。也优选HA蛋白的优选形式也具有本文所述一种或多种HA生物活性。
所述HA多肽或其生物活性部分可以与一种非HA多肽有效地连接,形成融合蛋白。在优选实施方案中,这种融合蛋白具有不同于单独的所述HA蛋白的活性。在其它优选实施方案中,这种融合蛋白参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能。在特别优选的实施方案中,这种融合蛋白整合到宿主细胞中,调节所述细胞的所需化合物产生。
另一方面,本发明提供用于筛选调节HA蛋白活性的分子的方法,所述分子或者通过与HA蛋白本身相互作用或者作为所述HA蛋白的底物或结合配偶体,或者通过调节本发明HA核酸分子的转录或翻译,调节HA蛋白活性。
本发明的另一方面涉及用于生产精细化学品的方法。该方法包括培养含指导本发明HA核酸分子表达的载体的细胞,使得可生产精细化学品。在一个优选实施方案中,该方法还包括获得含这种载体的细胞的步骤,其中用指导HA核酸表达的载体转染细胞。在另一优选实施方案中,该方法还包括从培养物中回收所述精细化学品的步骤。在一个特别优选的实施方案中,所述细胞得自棒杆菌属或短杆菌属,或选自表3中所述的那些菌株。
本发明的另一方面涉及用于调节由微生物生产分子的方法。这类方法包括使所述细胞与调节HA蛋白活性或HA核酸表达的因子接触,使得细胞相关活性相对于在缺乏所述因子时的所述活性而言被改变。在一个优选实施方案中,调节所述细胞参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或酶活性或蛋白水解活性的一个或多个过程。调节HA蛋白活性的因子可以是刺激HA蛋白活性或HA核酸表达的因子。刺激HA蛋白活性或HA核酸表达的因子的实例包括小分子、活性HA蛋白和已被导入所述细胞的编码HA蛋白的核酸。抑制HA活性或表达的因子的实例包括小分子和反义HA核酸分子。
本发明的另一方面涉及调节细胞的所需化合物收率的方法,所述方法包括将野生型或突变型HA基因导入细胞中,或者保留在单独的质粒上或整合到宿主细胞的基因组中。如果整合到基因组中,则这种整合可以是随机的,或它可以通过同源重组而发生,使得所述天然基因被所导入的拷贝取代,导致从待调节的细胞产生所述所需化合物。在一个优选实施方案中,所述收率增加。在另一优选实施方案中,所述化学品是一种精细化学品。在一个特别优选的实施方案中,所述精细化学品是一种氨基酸。在尤其优选的实施方案中,所述氨基酸是L-赖氨酸。发明详述
本发明提供HA核酸分子和HA蛋白分子,所述分子参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种谷氨酸棒杆菌酶活性或蛋白水解活性。本发明的分子可以用于或者直接调节微生物例如谷氨酸棒杆菌的精细化学品生产(例如其中参与精细化学品(例如一种酶)生产的蛋白质的过量表达或其活性的优化对于经修饰的谷氨酸棒杆菌的精细化学品产生的收率、产量和/或产率有直接影响),或可以具有间接影响,无论如何这都导致所需化合物生产的收率、产量和/或产率的提高(例如其中对谷氨酸棒杆菌芳族或脂族修饰或降解蛋白的活性或拷贝数的调节,导致谷氨酸棒杆菌细胞生存力的提高,这进而使得大规模培养环境中产量的增加)。以下进一步说明本发明的各个方面。I.精细化学品
术语“精细化学品”是本领域公知的,包括由生物产生的在各种工业(例如但不限于制药业、农业和化妆品工业)上具有多种应用的分子。这类化合物包括有机酸,例如酒石酸、衣康酸和二氨基庚二酸;生成蛋白质的和非生成蛋白质的氨基酸;嘌呤和嘧啶碱基、核苷和核苷酸(如描述于例如Kuninaka,A.(1996)核苷酸和相关化合物,载于Biotechnology,第6卷,第561-612页,Rehm等编著,VCH:Weinheim以及其中含有的参考文献);脂质、饱和和不饱和脂肪酸(例如花生四烯酸);二元醇(例如丙二醇和丁二醇);糖类(例如透明质酸和海藻糖);芳族化合物(例如芳香胺、香草醛和靛蓝);维生素和辅因子(如描述于Ullmann’s Encyclopedia of Industrial Chemistry,第A27卷,“Vitamins”,第443-613页(1996)VCH:Weinheim以及其中的参考文献;和Ong,A.S.,Niki,E.和Packer,L.(1995)“Nutrition,Lipids,Health,and Disease”Proceedings of the UNESCO/Confederation of Scientific and TechnologicalAssociations in Malaysia,and the Society for Free Radical Research-Asia,于1994年9月1-3日在马来西亚Penang举行,AOCS Press,(1995));酶;聚酮化合物(Cane等(1998)Science 282:63-68);和描述于Gutcho(1983)Chemicals by Fermentation,Noyes Data Corporation,ISBN:0818805086中以及其中的参考文献的所有其它化学品。以下进一步详细描述这些精细化学品中的某些的代谢和应用。A.氨基酸代谢和应用
氨基酸包括所有蛋白质的基本结构单元,因此是所有生物的正常细胞功能必不可少的。术语“氨基酸”是本领域公知的。生成蛋白质的氨基酸有20种,用作蛋白质的结构单元,在蛋白质中它们通过肽键连接;而非蛋白生成的氨基酸(已知数百种所述氨基酸)不是在蛋白质中正常发现的(参见Ulmann’s Encyclopedia of Industrial Chemistry,第A2卷,第57-97页VCH:Weinheim(1985))。氨基酸可以具有D-或L-光学构型,虽然L-氨基酸一般是天然存在的蛋白质中发现的唯一类型。20种生成蛋白质的氨基酸中每种的生物合成和降解途径已经在原核细胞和真核细胞中很好地表征了(参见例如Stryer,L.Biochemistry,第3版,第578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)如此命名,是因为它们的生物合成复杂,一般是营养上需要的,所述“必需”氨基酸容易通过简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物的确保留合成这些氨基酸中的某些氨基酸,但必需氨基酸必需从饮食中供应,以便进行正常的蛋白质合成。
除了这些氨基酸在蛋白质生物合成中的功能外,这些氨基酸自身还是令人感兴趣的化学品,已经发现其中许多氨基酸在食品工业、饲料工业、化学工业、化妆品工业、农业和制药业中具有各种应用。赖氨酸不仅是人类营养的重要氨基酸,而且也是单胃动物(例如家禽和猪)营养的重要氨基酸。谷氨酸最常用作调味添加剂(谷氨酸一钠,MSG),并且在食品工业广泛使用,天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸都用于制药业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸在制药业和化妆品工业上有价值。苏氨酸、色氨酸和D/L-甲硫氨酸是常用的饲料添加剂。(Leuchtenberger,W.(1996)氨基酸-技术生产和应用,载于Rehm等(编著)Biotechnology,第6卷,第14a章,第466-502页,VCH:Weinheim)。另外,已经发现这些氨基酸可用作合成氨基酸和蛋白质合成的前体,例如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟基色氨酸以及描述于Ulmann’s Encyclopedia of IndustrialChemistry,第A2卷,第57-97页,VCH:Weinheim,1985的其它氨基酸。
在能够生产这些天然氨基酸的生物(例如细菌)体内的这些天然氨基酸的生物合成已经很好地表征(有关细菌氨基酸生物合成及其调节的综述,参见Umbarger,H.E.(1978)Ann.Rev.Biochem.47:533-606)。通过柠檬酸循环中的中间体α-酮戊二酸的还原酰胺化,合成谷氨酸。随后由谷氨酸分别产生谷氨酰胺、脯氨酸和精氨酸。丝氨酸的生物合成是一个三步骤过程,以3-磷酸甘油酸(糖酵解中的中间体)开始,在氧化、转氨基作用和水解步骤后,产生这种氨基酸。半胱氨酸和甘氨酸都由丝氨酸产生,前者通过高半胱氨酸和丝氨酸缩合而产生,而后者通过在一个由丝氨酸转羟甲基酶催化的反应中将侧链β-碳原子转移至四氢叶酸而产生。苯丙氨酸和酪氨酸在预苯酸合成后在仅最后两个步骤不同的9步骤生物合成途径中,由糖酵解和戊糖磷酸途径前体4-磷酸赤藓糖和磷酸烯醇丙酮酸合成。色氨酸也由这两种起始分子产生,但其合成是一个11步骤途径。酪氨酸也可以在一个由苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸都是糖酵解终产物-丙酮酸的生物合成产物。天冬氨酸由柠檬酸循环的中间体草酰乙酸生成。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸分别通过天冬氨酸的转化而产生。异亮氨酸由苏氨酸生成。一个复杂的9步骤途径导致由一种活化糖-5-磷酸核糖-1-焦磷酸产生组氨酸。
细胞的过量蛋白质合成需求中的氨基酸不能被贮存,而是被降解,为细胞的主要代谢途径提供中间体(有关综述参见Stryer,L.Biochemistry,第3版,第21章,“氨基酸降解和尿素循环”,第495-516页(1988))。虽然细胞能够将不想要的氨基酸转化为有用的代谢中间体,但就能量、前体分子和合成氨基酸所必需的酶而言,氨基酸生产是昂贵的。因此,氨基酸生物合成受反馈抑制调节是不奇怪的,在反馈抑制中,特定氨基酸的存在起减慢或完全终止其自身产生的作用(有关氨基酸生物合成途径中的反馈机制的综述,参见Stryer,L.Biochemistry,第3版,第24章,“氨基酸和血红素的生物合成”,第575-600页(1988))。因此,任何特定氨基酸的输出受细胞中存在的氨基酸量的限制。B.维生素、辅因子和营养药的代谢和应用
维生素、辅因子和营养药包括高等动物已经丧失合成能力并且因此必须摄入的另外一组分子,尽管它们容易由其它生物例如细菌合成。这些分子或者本身是生物活性物质,或者是可以用作用作电子载体或多种代谢途径的中间体的生物活性物质的前体。这些化合物除了具有营养价值外,也具有作为着色剂、抗氧化剂和催化剂或其它加工助剂的重要工业价值。(有关这些化合物的结构、活性和工业应用的概述,参见例如Ullman’s Encyclopedia of Industrial Chemistry,“Vitamins”,第A27卷,第443-613页,VCH:Weinheim,1996)。术语“维生素”是本领域公知的,包括生物正常功能所需、但生物自身不能合成的营养物。维生素类可以包括辅因子和营养药化合物。用语“辅因子”包括发生正常酶活性所需的非蛋白性化合物。这类化合物可以是有机化合物或无机化合物;本发明的辅因子分子最好是有机分子。术语“营养药”包括在植物和动物、特别是人类中具有健康益处的食物增补剂。这类分子的实例是维生素、抗氧化剂以及某些脂质(例如多不饱和脂肪酸)。
已经大量表征了这些分子在能够生产其的生物例如细菌中的生物合成(Ullman’s Encyclopedia of Industrial Chemistry,“Vitamins”,第A27卷,第443-613页,VCH:Weinheim,1996;Michal,G.(1999)Biochemical Pathways:An Atlas of Biochemistry and Molecular Biology,John Wiley & Sons;Ong,A.S.,Niki,E和Packer,L.(1995)“Nutrition,Lipids,Health,and Disease”Proceedings of the UNESCO/Confederationof Scientific and Technological Associations in Malaysia,and the Societyfor Free Radical Research-Asia,于1994年9月1-3日在马来西亚Penang举行,AOCS Press:Champaign,IL X,374 S)。
通过将嘧啶和噻唑部分化学偶联,产生硫胺素(维生素B1)。核黄素(维生素B2)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素进而用来合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。统称为“维生素B6”的化合物家族(例如吡哆醇、吡哆胺、吡哆醛-5’-磷酸以及商业上使用的盐酸吡哆醇)都是具有共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸((R)-(+)-N-(2,4-二羟基-3,3-二甲基-1-氧代丁基)-β-丙氨酸)可以或者通过化学合成或者通过发酵生产。泛酸生物合成中的最后的步骤由ATP驱动的β-丙氨酸和泛解酸缩合组成。负责转化为泛解酸、β-丙氨酸和转化为泛酸的缩合的生物合成步骤的酶是已知的。代谢活性形式的泛酸是辅酶A,其生物合成以5个酶促步骤进行。泛酸、吡哆醛-5’-磷酸、半胱氨酸和ATP是辅酶A的前体。这些酶不仅催化泛酸的生成,而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(前维生素B5)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。
已经详细研究了微生物中由前体分子庚二酰辅酶A开始的生物素生物合成,并且已经鉴定出所涉及的几种基因。已经发现许多相应的蛋白质也参与铁簇合成,并且是nifS类蛋白质的成员。硫辛酸衍生自辛酸,用作能量代谢中的辅酶,在能量代谢中它成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸类是一类叶酸衍生物的物质,叶酸又衍生自L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已经在某些微生物中详细地研究了叶酸及其衍生物的生物合成,所述生物合成起始于代谢中间体鸟苷-5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸。
类咕啉(例如钴胺素、特别是维生素B12)和卟啉属于一类特征为四吡咯环系的化学物质。维生素B12的生物合成足够复杂,以致尚未被完全鉴定,但现在已知许多所涉及的酶和底物。烟酸和烟酰胺是吡啶衍生物,也称为“尼克酸”。烟酸是重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)及其还原形式的前体。
这些化合物的大规模生产一直主要依靠无细胞化学合成,尽管这些化学品中的某些也已经通过大规模微生物培养来生产,例如核黄素、维生素B6、泛酸和生物素。仅维生素B12由于其合成的复杂性通过发酵生产。体外方法花费大量原料和时间,通常费用巨大。C.嘌呤、嘧啶、核苷和核苷酸的代谢和应用
嘌呤和嘧啶代谢基因及其相应的蛋白质是肿瘤疾病和病毒感染的重要的治疗靶。用语“嘌呤”或“嘧啶”包括含氮碱基,它们是核酸、辅酶和核苷酸的组分。术语“核苷酸”包括核酸分子的基本结构单元,它们由一个含氮碱基、一个戊糖(在RNA的情况下,所述糖是核糖;在DNA的情况下,所述糖是D-脱氧核糖)和磷酸构成。用语“核苷”包括用作核苷酸前体、但缺乏核苷酸所具有的磷酸部分的分子。通过抑制这些分子的生物合成或抑制其形成核酸分子的转移,有可能抑制RNA和DNA的合成;通过以靶向癌细胞的方式抑制这种活性,可以抑制肿瘤细胞分裂和复制的能力。另外,有不形成核酸分子、而是用作能量贮存(即AMP)或用作辅酶(即FAD和NAD)的核苷酸。
几个出版物已经描述了通过影响嘌呤和/或嘧啶代谢,将这些化学物质用于这些医学适应征(例如Christopherson,R.I.和Lyons,S.D.(1990)“作为化疗药的嘧啶和嘌呤从头生物合成的有效抑制剂”,Med.Res.Reviews 10:505-548)。对参与嘌呤和嘧啶代谢的酶的研究一直集中于可以用作例如免疫抑制剂或抗增殖药的新药的开发(Smith,J.L.,(1995)“核苷酸合成中的酶”,Curr.Opin.Struct.Biol.5:752-757;(1995)Biochem Soc.Transact.23:877-902)。然而,嘌呤和嘧啶碱基、核苷和核苷酸具有其它用途:用作几种精细化学品(例如硫胺素、S-腺苷-甲硫氨酸、叶酸或核黄素)生物合成的中间体、用作细胞的能量载体(例如ATP或GTP)和用于化学品自身,通常用作黄素增强剂(例如IMP或GMP)或用于几种药物应用(参见例如Kuninaka,A.(1996)Nucleotidesand Related Compounds in Biotechnology,第6卷,Rehm等编著,VCH:Weinheim,第561-612页)。此外,参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作开发用于保护作物的化学品的靶,所述化学品包括杀真菌剂、除草剂和杀虫剂。
已经鉴定了这些化合物在细菌中的代谢(有关综述参见例如Zalkin,H.和Dixon,J.E.(1992)“嘌呤核苷酸的从头生物合成”,载于:Progress in Nucleic Acid Research and Molecular Biology,第42卷,Academic Press,第259-287页;和Michal,G.(1999)“核苷酸和核苷”,载于:Biochemical Pathways:An Atlas of Biochemistry and MolecularBiology,第8章,Wiley:New York)。嘌呤代谢一直是广泛研究的主题,并且对于细胞的正常功能是必不可少的。在高等动物中嘌呤代谢受损可以引起严重的疾病,例如痛风。嘌呤核苷酸由核糖-5-磷酸开始合成,通过中间体化合物肌苷-5’-磷酸(IMP)的一系列步骤,导致鸟苷-5’-一磷酸(GMP)或腺苷-5’-一磷酸(AMP)的产生,由GMP或AMP容易生成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存,因为其降解为细胞中的许多不同生物化学过程提供能量。嘧啶生物合成通过由核糖-5-磷酸形成尿苷-5’-一磷酸(UMP)而进行。UMP进而转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式在一步还原反应中产生,从所述核苷酸的二磷酸核糖形式还原反应为所述核苷酸的二磷酸脱氧核糖形式。在磷酸化后,这些分子能够参与DNA合成。D.海藻糖的代谢和应用
海藻糖由以α,α-1,1-键合连接的两个葡萄糖分子组成。海藻糖在食品工业中通常用作甜味剂,即用于干燥食品或冷冻食品和饮料中的一种添加剂。然而,它在制药业、化妆品工业和生物技术工业中也具有许多应用(参见例如Nishimoto等,(1998)美国专利号5,759,610;Singer,M.A.和Lindquist,S.(1998)Trends Biotech.16:460-467;Paiva,C.L.A.和Panek,A.D.(1996)Biotech.Ann.Rev.2:293-314;和Shiosaka,M.(1997)J.Japan 172:97-102)。海藻糖用来自许多微生物的酶来生产,并且天然被释放到周围介质中,可以用本领域已知的方法从所述周围介质中收集海藻糖。II.谷氨酸棒杆菌内稳态的维持和对环境的适应
细胞进行的代谢过程和其它生化过程对环境条件例如温度、压力、溶质浓度和氧可得性敏感。当一种或多种这些环境条件被干扰或改变的方式与这些细胞过程正常功能不相容时,细胞必须采取行动以维持胞内环境,无论胞外环境有多么不利,这都将使得它们能够生存。革兰氏阳性细菌细胞例如谷氨酸棒杆菌细胞有许多机制可以维持内环境稳定,无论胞外环境有多不利。这些包括细胞壁、能够降解可能的毒性芳族和脂族化合物的蛋白质、可以快速破坏错折叠或受错误调节的蛋白质的蛋白水解机制以及允许在细菌生长最适条件下通常不发生的胞内反应发生的催化剂。
除了仅在不利环境中生存外,细菌细胞(例如谷氨酸棒杆菌细胞)也通常能够适应,使得它们能够利用这类条件。例如,在缺乏所需碳源的环境中细胞能够适应在不太适合的碳源上生长。此外,当通常利用的无机化合物不可得到时,细胞可能能够利用不太理想的无机化合物。谷氨酸棒杆菌细胞具有大量基因,使得它们能够适应以利用它们在最适生长条件下通常不会遇到的无机分子和有机分子,作为养分和代谢前体。以下进一步阐述参与稳态和适应的细胞过程的各个方面。A.芳族化合物和脂族化合物的修饰和降解
细菌细胞通常接触自然界中各种各样的芳族化合物和脂族化合物。芳族化合物是具有环状环结构的有机分子,而脂族化合物是具有开链结构而非具有环结构的有机分子。这类化合物可以作为工业加工的副产物(例如苯或甲苯)出现,但也可以由某些微生物产生(例如醇类)。这些化合物中的许多对细胞有毒,特别是芳族化合物,芳族化合物由于高能环结构而具有高度反应性。因此,某些细菌已经发展出它们能够修饰或降解这些化合物的机制,使得所述化合物对细胞不再有毒。细胞可能具有能够将芳族或脂族化合物例如羟化、异构化或甲基化的酶,使得它们或者毒性降低,或者使得经修饰的形式能够通过标准细胞废物和降解途径加工。另外,细胞可能具有能够特异性降解一种或多种这类潜在有害物质的酶,由此保护细胞。细菌中这些类型的修饰和降解过程的原理和实例在几个出版物中有描述,例如Sahm,H.(1999)“工业生产中的原核生物”,载于Lengeler,J.W.等编著,Biologyof the Procaryotes,Thieme Verlag:Stuttgart;和Schlegel,H.G.(1992)Allgemeine Mikrobiologie,Thieme:Stuttgart)。
除了简单地将有害芳族或脂族化合物失活外,许多细菌已经进化得使其在细胞的优选碳源不能得到时能够利用这些化合物作为碳源进行连续代谢。例如,已知假单胞菌属菌株能够利用甲苯、苯和1,10-二氯癸烷作为碳源(Chang,B.V.等(1997)Chemosphere 35(12):2807-2815;Wischnak,C.等(1998)Appl.Environ.Microbiol.64(9):3507-3511;Churchill,S.A.等(1999)Appl.Environ.Microbiol.65(2):549-552)。本领域已知有来自许多其它细菌菌种的相似实例。
某些细菌修饰或降解芳族和脂族化合物的能力已经开始得到利用。石油是一种复杂的化学物质的混合物,它包括脂族分子和芳族化合物。例如通过应用有能力将这些有毒化合物降解或修饰成为浮油的细菌,有可能高效率和低成本地消除许多环境损害(参见例如Smith,M.R.(1990)“细菌对芳烃的生物降解”Biodegradation 1(2-3):191-206;和Suyama,T.等(1998)“降解脂族聚碳酸酯的细菌分离物”,FEMSMicrobiol.Lett.161(2):255-261)。B.无机化合物的代谢
细胞(例如细菌细胞)含有大量的不同分子,例如水、无机离子和有机物质(例如蛋白质、糖类和其它大分子)。大多数典型的细胞物质仅由四种类型的原子-碳、氧、氢和氮构成。虽然无机物质代表较小百分比的细胞内容物,但它们与细胞的正常功能等同重要。这类分子包括磷、硫、钙、镁、铁、锌、锰、铜、钼、钨和钴。这些化合物中的许多对于重要分子例如核苷酸(磷)和氨基酸(氮和硫)的构成至关重要。这些无机离子中的其它离子用作酶促反应的辅因子或影响渗透压。所有这类分子必须由细菌从周围环境中吸收。
对于每种这些无机化合物,理想的是细菌吸收可以通过细胞的标准代谢机器最为快速利用的形式。然而,细菌可能遭遇这些优选形式不容易获得的环境。为了在这些环境下生存,对于细菌而言重要的是具有额外的能够将这些无机化合物代谢上活性较低但容易获得的形式转化为在细胞代谢中可以使用形式的生化机制。细菌通常具有大量编码用于这种目的的酶的基因,这些基因在不能获得所需无机种类时才表达。因此,各种无机化合物代谢的这些基因作为细菌可以用来适应亚适环境条件的另一工具。
在碳之后,细胞中最为重要的元素是氮。典型的细菌细胞含有12-15%的氮。它是氨基酸和核苷酸以及细胞中许多其它重要分子的组分。此外,氮可以替代氧作为能量代谢中的末端电子受体。好的氮源包括许多有机化合物和无机化合物,例如氨气或铵盐(例如
、
或
)、硝酸盐、尿素、氨基酸或复合氮源如玉米浆、大豆粉、大豆蛋白、酵母膏、肉膏等。氨的氮通过特定酶的作用而得以固定:谷氨酸脱氢酶、谷氨酰胺合酶和谷氨酰胺-2-酮戊二酸氨基转移酶。氨基氮从一种有机分子转移至另一种有机分子由氨基转移酶来完成,氨基转移酶是一类将一个氨基从α-氨基酸转移至α-酮酸的酶。硝酸盐可以通过硝酸还原酶、亚硝酸还原酶和其它氧化还原酶来还原,直至它转化为它可以被细胞在标准代谢途径中容易利用的分子氮或氨。
磷通常在细胞内以有机形式和无机形式发现,并且可以由细胞以或这些形式中的任一种吸收,尽管大多数微生物优先吸收无机磷酸。有机磷酸转化为细胞可以利用的形式,需要磷酸酶(例如肌醇六磷酸酶,它水解肌醇六磷酸,产生磷酸和肌醇衍生物)的作用。磷酸是核酸合成中的关键成分,在细胞能量代谢中也具有重要作用(例如在ATP、ADP和AMP的合成中)。
硫是氨基酸(例如甲硫氨酸和半胱氨酸)、维生素(例如硫胺素、生物素和硫辛酸)和铁硫蛋白合成所需的。细菌主要从无机硫酸获得硫,尽管也通常利用硫代硫酸、亚硫酸和硫化物。在这些化合物不容易获得的条件下,许多细菌表达能够使其利用磺酸化合物例如2-氨基磺酸(牛磺酸)的基因(Kertesz,M.A.(1993)“大肠杆菌、恶臭假单胞菌(Pseudomonas putida)或金黄色葡萄球菌(Staphylococcus aureus)中由硫酸限制诱导的蛋白质”,J.Bacteriol.175:1187-1190)。
其它无机原子,例如金属离子或钙离子,对于细胞生存力也是重要的。铁例如在氧化还原反应中具有重要作用,并且是铁硫蛋白、血红素蛋白和细胞色素的辅因子。将铁吸收到细菌细胞中可以通过铁载体的作用来完成,铁载体是结合胞外铁离子并且将它们转运至细胞内部的螯合剂。有关铁和其它无机化合物代谢的参考文献,参见:Lengeler等(1999)Biology of Prokaryotes,Thieme Verlag:Stuttgart;Neidhardt,F.C.;等编著Escherichia coli and Salmonella,ASM Press:Washington,D.C.;Sonenshein,A.L.等编著(199?)Bacillus subtilis and Other Gram-PositiveBacteria,ASM Press:Washington,D.C.;Voet,D.和Voet,J.G.(1992)Biochemie,VCH:Weinheim;Brock,T.D.和Madigan,M.T.(1991)Biologyof Microorgansisms,第6版,Prentice Hall:Englewood Cliffs,第267-269页;Rhodes,P.M.和Stanbury,P.F.Applied Microbial Physiology-APractical Approach,Oxford Univ.Press:Oxford。C.酶和蛋白水解
对于细菌例如谷氨酸棒杆菌优化的胞内条件通常不是许多生化反应通常发生的条件。为了使这些反应在生理条件下进行,细胞利用酶。酶是蛋白性生物催化剂,是空间上定向反应的分子,或提供特化环境,使得生化反应的能障降低。不同的酶催化不同的反应,每种酶可以是转录调节、翻译调节或翻译后调节的主体,使得该反应仅在合适的条件和特定时间发生。酶可以导致化合物降解(例如蛋白酶)、合成(例如合酶)或修饰(例如转移酶或异构酶),所有这些都使得在细胞内能够产生必需化合物。这进而导致维持细胞的自动调节。
然而,酶对于细菌最具活力的生理条件下的活性是最适的,这一事实意味着当环境条件受到干扰时,很有可能也将干扰该酶的活性。例如,温度的改变可能导致异常折叠的蛋白质,pH的改变也是如此-蛋白质折叠主要取决于多肽链内氨基酸的静电作用和疏水作用,因此多肽链内各个氨基酸电荷的任何改变(可能由于细胞pH的改变而引起)对于蛋白质正确折叠的能力可以具有深远的影响。温度的改变有效地改变多肽分子所具动能的量,这影响多肽成为正确折叠的、能量学稳定构型的能力。错折叠蛋白质可能对细胞有害有两个原因。第一,异常折叠的蛋白质可能具有同样异常活性,或根本没有活性。第二,错折叠蛋白质可能缺乏受其它细胞系统正常调节所必需的构象区,因此可能继续有活性,但是以一种不受控制的方式有活性。
细胞有一种机制可以在发生对细胞的任何损害之前快速破坏错折叠的酶和调节蛋白:蛋白水解。蛋白质例如la/lon家族的那些蛋白质和Clp家族的那些蛋白质特异性地识别和降解错折叠蛋白质(参见例如Sherman,M.Y.,Goldberg,A.L.(1999)EXS 77:57-78和其中的参考文献以及Porankiewicz J.(1999)Molec.Microbiol.32(3):449-58和其中的参考文献;Neidhardt,F.C.等(1996)E.coli and Salmonella,ASM Press:Washington,D.C.和其中的参考文献;和Pritchard,G.G.和Coolbear,T.(1993)FEMS Microbiol.Rev.12(1-3):179-206和其中的参考文献)。这些酶与错折叠或未折叠的蛋白质结合并且以ATP依赖性方式将其降解。因此,蛋白水解用作细胞用来防止环境改变时损害正常细胞功能的重要机制,它还在于其它情况下由于受错误调节和/或异常酶活性或调节活性而有毒的条件和环境中使得细胞能够存活。
蛋白水解在细胞中在最适环境条件下也具有重要功能。在正常代谢过程中,蛋白酶帮助肽键的水解、复杂分子的分解代谢,以提供必需的降解产物,以及帮助蛋白修饰。分泌的蛋白酶甚至在外部养分进入细胞之前在这些化合物分解代谢中具有重要作用。此外,蛋白酶活性本身可以作为调节功能;已知枯草杆菌中的孢子形成和柄杆菌(Caulobacter spp.)中的细胞周期进程受每一这些菌种中关键蛋白水解事件的调节(Gottesman,S.(1999)Curr.Opin.Microbiol.2(2):142-147)。因此,蛋白水解过程对于亚适和最适环境条件下细胞的生存都是重要的,并且影响细胞内稳态的总体维持。D.细胞壁的产生和重排
虽然细胞的生化机器能够容易地适应不同的可能不利的环境,但细胞仍需要可以保护它们抵抗环境的通用机制。对于许多细菌而言,细胞壁提供这种保护,也在附着、细胞生长和分裂以及所需溶质和废物转运方面起作用。
为了执行其功能,细胞需要代谢物和其它分子的胞内浓度基本上高于周围介质的浓度。因为这些代谢物由于疏水膜的存在而基本上不能离开细胞,所以该系统的倾向使水分子从外部介质进入细胞,使得溶质的内部浓度与外部浓度匹配。水分子能够容易地越过细胞膜,该膜不能抵抗所引起的膨胀和压力,这可能导致细胞的渗透裂解。细胞壁的刚性大大提高了细胞耐受这些压力的能力,并且提供了对这些代谢物和所需溶质从细胞中不希望有的扩散的另一道屏障。同样,细胞壁也用来防止不想要的物质进入细胞。
细胞壁也参与许多其它细胞过程,例如附着以及细胞生长和细胞分裂。由于细胞壁完全围绕细胞的这一事实,所以细胞与其周围物质的任何相互作用必定由细胞壁介导。因此,细胞壁必须参与细胞与其它细胞以及所需表面的任何附着。此外,如果细胞壁没有同时改变,则细胞不能生长或分裂。由于细胞壁所提供的这种保护需要在生长、形态发生和繁殖期间细胞壁存在,因此细胞分裂中的关键步骤之一是细胞内的细胞壁合成,使得从老细胞分裂出新细胞。因此,通常细胞壁的生物合成与细胞生长和细胞分裂受到衔接调节(参见例如Sonenshein,A.L.等编著(1993)Bacillus subtilis and Other Gram-PositiveBacteria,ASM:Washington,D.C.)。
细胞壁的结构在革兰氏阳性细菌和革兰氏阴性细菌之间是不同的。然而,在这两种类型中,细胞壁的基本结构单位仍是相似的:两种多糖-N-乙酰葡糖胺(NAG)和N-乙酰胞壁酸(NAM)的重叠网格,这两种多糖通过氨基酸(最常为L-丙氨酸、D-谷氨酸、二氨基庚二酸和D-丙氨酸)交联,称为“肽聚糖”。已知参与细胞壁合成的过程(参见例如Michal,G.编著(1999)Biochemical Pathways:An Atlas ofBiochemistry and Molecular Biology,Wiley:New York)。
在革兰氏阴性细菌中,细胞内膜包有单层肽聚糖(约10nm厚),称为胞壁质-小囊。这种肽聚糖结构非常坚硬,其结构决定了该生物的形状。胞壁质-小囊的外表面覆盖一层含有膜孔蛋白和其它膜蛋白、磷脂和脂多糖的外膜。为了维持与外膜的紧密结合,革兰氏阴性细菌的细胞壁也具有散布的脂质分子,用来将其锚定至环绕的膜上。
在革兰氏阳性细菌例如谷氨酸棒杆菌中,质膜覆盖有多层的肽聚糖,其厚度为20-80nm不等(参见例如Lengeler等(1999)BiologyProkaryotes Thieme Verlag:Stuttgart,第913-918页、第875-899页和第88-109页以及其中的参考文献)。革兰氏阳性细菌的细胞壁也含有磷壁酸,这是一种通过磷酸酯基连接的甘油或核糖醇的聚合物。磷壁酸也能够与氨基酸连接,而与胞壁酸形成共价键。在细胞壁中也可能存在脂磷壁酸和糖醛酸磷壁酸。诸如鞭毛或夹膜之类的细胞表面结构如果有的话,也将锚定在该层中。III.本发明的元件和方法
本发明至少部分基于本文称为HA核酸分子和HA蛋白分子的新分子的发现,所述HA分子参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能。在一个实施方案中,HA分子参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种酶活性或蛋白水解活性。在一个优选实施方案中,本发明的HA分子有关谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或酶活性或蛋白水解活性的活性对该生物产生所需精细化学品有影响。在一个特别优选的实施方案中,本发明的HA分子在活性上受调节,使得所述HA分子参与的谷氨酸棒杆菌细胞过程(例如谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或酶活性或蛋白水解活性)也在活性上被改变,导致或者直接或者间接地调节谷氨酸棒杆菌生产所需精细化学品的收率、产量和/或产率。
用语“HA蛋白”或“HA多肽”包括参与同谷氨酸棒杆菌稳态或谷氨酸棒杆菌细胞对不利环境条件适应能力有关的多种细胞过程的蛋白质。例如,HA蛋白可以参与谷氨酸棒杆菌细胞壁生物合成或重排、谷氨酸棒杆菌中无机化合物的代谢、谷氨酸棒杆菌中芳族或脂族化合物的修饰或降解、或具有一种谷氨酸棒杆菌酶活性或蛋白水解活性。HA蛋白的实例包括由表1中所述的HA基因和奇数SEQ ID NO编码的那些蛋白质。术语“HA基因”或“HA核酸序列”包括编码HA蛋白、由一个编码区以及相应的非翻译5’和3’序列区组成的核酸序列。HA基因的实例包括表1中所述的那些基因。术语“产量”或“生产力”是本领域公知的,包括在给定时间和给定发酵体积内生成的发酵产物(例如所需精细化学品)的浓度(例如每小时每升的kg产物)。术语“产率”包括待达到的特定生产水平所需的时间(例如细胞达到特定精细化学品产出率需要多长时间)。术语“收率”或“产物/碳收率”是本领域公知的,包括碳源转化为产物(即精细化学品)的效率。这通常表示为例如每kg碳源的kg产物。通过增加所述化合物的收率或产量,增加在给定时间给定量的培养物中该化合物的回收分子的量或可用回收分子的量。术语“生物合成”或“生物合成途径”是本领域公知的,包括细胞在可以多步骤且高度受调节的过程中从中间体化合物开始的化合物、最好是有机化合物的合成。术语“降解”或“降解途径”是本领域公知的,包括细胞在可以是多步骤且高度受调节的过程中将化合物、最好是有机化合物分解为降解产物(一般而言,是较小或复杂度较低的分子)。用语“代谢”是本领域公知的,包括生物体内发生的全部生物化学反应。特定化合物的代谢(例如诸如甘氨酸的氨基酸的代谢)则包括细胞中与该化合物有关的全部生物合成、修饰和降解途径。术语“稳态”是本领域公知的,包括细胞所用的无论普遍的胞外环境条件如何都可维持恒定的胞内环境的所有机制。这类过程的非限制性实例是利用细胞壁防止胞内高溶质浓度所致的渗透裂解。术语“适应”或“对环境条件的适应”是本领域公知的,包括细胞用来使细胞能够在非优选环境条件(一般而言,是其中一种或多种合适养分缺乏的那些环境条件,或其中诸如温度、pH、重量摩尔渗透压浓度、氧百分比等落在细胞最适生存范围之外的环境条件)存活的机制。许多细胞包括谷氨酸棒杆菌细胞具有编码在这类环境条件下表达并且使其在这类亚适条件下继续生长的蛋白质的基因。
在另一实施方案中,本发明的HA分子能够调节微生物例如谷氨酸棒杆菌中所需分子例如精细化学品的产生。有许多的机制使得本发明HA蛋白的改变可以直接影响掺入这种改变的蛋白的谷氨酸棒杆菌菌株生产精细化学品的收率、产量和/或产率。例如,通过对修饰或降解芳族或脂族化合物的酶进行工程改造,使得这些酶的活性或数量增加或降低,有可能对作为这些化合物的修饰产物或降解产物的一种或多种精细化学品的产生进行调节。同样,参与无机化合物代谢的酶为诸如氨基酸、维生素和核酸之类的精细化学品的生物合成提供关键分子(例如磷、硫和氮分子)。通过改变谷氨酸棒杆菌中这些酶的活性或数量,有可能增加这些无机化合物的转化(或使用替代无机化合物),由此使得这些精细化学品中无机原子掺入的速率提高。对参与普遍性细胞过程的谷氨酸棒杆菌酶进行基因工程,也可以直接改进精细化学品的产生,因为这些酶中的许多酶直接修饰精细化学品(例如氨基酸)或参与精细化学品合成或分泌的酶。对细胞蛋白酶活性或数量的调节也可能对精细化学品的产生具有直接影响,因为许多蛋白酶可以降解精细化学品或参与精细化学品产生或分解的酶。
此外,参与芳族/脂族化合物修饰或降解、一般性生物催化、无机化合物代谢或蛋白水解的上述酶本身均是精细化学品,在各种体外工业应用中需要其活性。通过改变谷氨酸棒杆菌中一种或多种这些酶的基因的拷贝数,有可能增加细胞产生的这些蛋白质的数量,由此提高大规模谷氨酸棒杆菌或相关细菌培养物生产这些蛋白质的潜在收率或产率。
本发明HA蛋白的改变也可能间接影响掺入这类经改变蛋白的谷氨酸棒杆菌菌株生产精细化学品的收率、产量和/或产率。例如,通过对参与细胞壁构建或重排的那些蛋白质的活性和/或数量进行调节,有可能修饰细胞壁本身的结构,致使细胞能够更好地经受住大规模发酵培养期间存在的机械应力和其它应力。另外,谷氨酸棒杆菌的大规模生长需要大量的细胞壁产生。对细胞壁生物合成酶或降解酶的活性或数量进行调节,可以使得细胞壁生物合成的速率更加快速,这进而可以使得培养物中该微生物的生长速率提高,由此增加生产所需精细化学品的细胞数量。
通过修饰本发明HA酶,也可以间接影响谷氨酸棒杆菌生产一种或多种精细化学品的收率、产量或产率。例如,谷氨酸棒杆菌中许多通用酶对通用(global)细胞过程(例如调节过程)具有重大影响,这进而对精细化学品代谢具有重大影响。同样,蛋白酶、修饰或降解可能的毒性芳族或脂族化合物的酶以及促进无机化合物代谢的酶均可用来提高谷氨酸棒杆菌的生存力。这些蛋白酶有助于选择性地除去错折叠或经错误调节的蛋白质,例如在大规模发酵罐培养期间可能遇到的相对胁迫的环境条件下存在的那些蛋白质。通过改变这些蛋白质,有可能进一步增强这种活性以及提高培养物中谷氨酸棒杆菌的生存力。芳族/脂族修饰或降解蛋白质不仅可以用来将这些废物化合物(这些化合物可能作为培养基中的杂质或细胞自身的废物而遇到)解毒,而且允许细胞在培养物中最适碳源有限时可利用替代碳源。通过增加其数量和/或活性,可以提高培养物中谷氨酸棒杆菌细胞的存活。本发明的无机代谢蛋白质为细胞供应所有蛋白质和核苷酸(这只是其中之一)合成所需的无机分子,因此对于细胞的总生存力是至关重要的。增加大规模培养物中生产一种或多种所需精细化学品的活细胞的数量,应该导致同时提高所述培养物中所述精细化学品生产的收率、产量和/或产率。
本发明的分离的核酸序列包含在谷氨酸棒杆菌菌株的基因组中,所述菌株可通过美国典型培养物保藏中心获得,保藏号为ATCC13032。所述分离的谷氨酸棒杆菌HA DNA的核苷酸序列和谷氨酸棒杆菌HA蛋白的预测氨基酸序列分别示于序列表中奇数SEQ ID NO和偶数SEQ ID NO中。进行计算分析,将这些核苷酸序列分类和/或鉴定为参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种谷氨酸棒杆菌酶活性或蛋白水解活性的蛋白质的编码序列。
本发明也涉及所具有的氨基酸与本发明氨基酸序列(例如序列表中的偶数SEQ ID NO的序列)基本上同源的蛋白质。本文所用的所具有的氨基酸序列与选定氨基酸序列基本上同源的蛋白质,与所选定氨基酸序列(例如所述完整的所选定氨基酸序列)至少约50%同源。所具有的氨基酸序列与选定氨基酸序列基本上同源的蛋白质,也可以与所选定氨基酸序列有至少约50-60%、优选至少约60-70%、更优选至少约70-80%、80-90%或90-95%、最优选至少约96%、97%、98%、99%或更高的同源性。
本发明的HA蛋白或其生物活性部分或片段可以参与维持谷氨酸棒杆菌内稳态或可以执行参与该微生物对不同环境条件适应的功能,或具有表1中所述的一种或多种活性。
在以下小节中更加详细地描述本发明的各个方面。A.分离的核酸分子
本发明的一个方面涉及编码HA多肽或其生物活性部分的分离的核酸分子、以及足以用作HA编码核酸(例如HA DNA)鉴定或扩增的杂交探针或引物的核酸片段。本文所用的术语“核酸分子”将是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)以及采用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’端和5’端的非翻译序列:基因编码区5’端上游的至少约100个核苷酸的序列和编码区3’端下游至少约20个核苷酸的序列。所述核酸分子可以是单链或双链,但最好是双链DNA。“分离的”核酸分子是与所述核酸天然来源中存在的其它核酸分子分离的核酸分子。最好是,“分离的”核酸不含所述核酸来源的生物的基因组DNA中天然邻接所述核酸的序列(即位于所述核酸5’端和3’端的序列)。例如,在各种实施方案中,分离的HA核酸分子可以含有小于约5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的在所述核酸来源的细胞(例如谷氨酸棒杆菌细胞)的基因组DNA中天然邻接所述核酸分子的核苷酸序列。此外,“分离的”核酸分子,例如DNA分子,可以基本上不含其它细胞物质,或当通过重组技术产生时不含培养基,或当化学合成时不含化学前体或其它化学物质。
本发明的核酸分子,例如具有序列表中奇数SEQ ID NO核苷酸序列的核酸分子或其部分,可以用标准分子生物学技术和本文提供的序列信息来分离。例如,可以用序列表中奇数SEQ ID NO序列之一的全部序列或部分序列作为杂交探针,采用用标准杂交技术(例如,描述于以下文献的技术:Sambrook,J.,Fritsh,E.F.和Maniatis,T.MolecularCloning:A Laboratory Manual.第2版,Cold Spring Harbor Laboratory,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,1989),可以从谷氨酸棒杆菌文库中分离出谷氨酸棒杆菌HA DNA。此外,包含本发明核酸序列(例如奇数SEQ ID NO)之一的全部或部分序列的核酸分子,可以采用基于该序列设计的寡核苷酸引物,通过聚合酶链式反应来分离(例如,包含本发明核酸序列之一的全部或部分序列的核酸分子(例如序列表的奇数SEQ ID NO)可以采用基于所述序列的设计的寡核苷酸引物通过聚合酶链式反应来分离)。例如,可以从正常的内皮细胞分离mRNA(例如通过Chirgwin等(1979)Biochemistry 18:5294-5299的异硫氰酸鈲萃取法),然后用反转录酶(例如莫洛尼MLV反转录酶,可得自Gibco/BRL,Bethesda,MD;或AMV反转录酶,可得自SeikagakuAmerica,Inc.,St.Petersburg,FL)制备DNA。可以基于序列表中所示的核苷酸序列之一,设计用于聚合酶链式反应扩增的合成寡核苷酸引物。用cDNA或者基因组DNA作为模板,用合适的寡核苷酸引物,按照标准PCR扩增技术,可以扩增本发明的核酸。将如此扩增的核酸克隆到合适的载体中,并且通过DNA序列分析进行鉴定。此外,对应于HA核苷酸序列的寡核苷酸可以通过标准合成技术,例如用自动DNA合成仪来制备。
在一个优选实施方案中,本发明的分离的核酸分子包含一种序列表中所示的核苷酸序列。序列表中所示的本发明的核酸序列对应于本发明的谷氨酸棒杆菌HA DNA。该DNA包含编码HA蛋白(即分别示于序列表奇数SEQ ID NO序列中的“编码区”)的序列以及5’非翻译序列和3’非翻译序列,所述非翻译序列也分别示于序列表中的奇数SEQ ID NO中。另一方面,所述核酸分子可以仅包含序列表核酸序列中任一序列的编码区。
为了进行这种应用,人们会理解,序列表中所示的每种核酸序列和氨基酸序列具有标识性RXA、RXN、RXS或RXC编号,所述编号具有后接5位数字的标识符“RXA”、“RXN”、“RXS”或“RXC”(即RXA02458、RXN00249、RXS00153或RXC00963)。每种所述核酸序列包含至多三个部分:一个5’上游区、一个编码区和一个下游区。这三个区中的每个区通过相同的RXA、RXN、RXS或RXC标识符来鉴别,以避免混淆。叙述“序列表中奇数序列之一”则是指序列表中任一核酸序列,它也可以根据其不同的RXA、RXN、RXS或RXC标识符来区分。这些序列中的每个序列的编码区被翻译为相应的氨基酸序列,所述氨基酸序列也示于序列表中,作为紧接相应核酸序列之后的偶数SEQ ID NO。例如,RXA02548的编码区示于SEQ ID NO:1中,而其编码的氨基酸序列示于SEQ ID NO:2中。本发明核酸分子的序列根据与它们所编码的氨基酸分子相同的RXA、RXN、RXS或RXC标识符来鉴别,使得可以容易地将它们相联系。例如,名为RXA02458、RXN00249、RXS00153和RXC00963的氨基酸序列分别是核酸分子RXA02458、RXN00249、RXS00153和RXC00963核苷酸序列编码区的翻译物。本发明的RXA、RXN、RXS和RXC核苷酸序列和氨基酸序列与其指定的SEQ ID NO之间的对应关系示于表1中。
本发明的几种基因是“F标志的基因”。F标志的基因包括表1中所示的在RXA、RXN、RXS或RXC标识符之前具有一个“F”的那些基因。例如,在表1中指定为“F RXA00249”的SEQ ID NO:5是一种F标志的基因,SEQ ID NO:11、15和33(在表1中分别指定为“F RXA02264”、“F RXA02274”和“F RXA00675”)也是如此。
在一个实施方案中,本发明的核酸分子不是指包括表2中编辑的那些核酸分子。就dapD基因而言,该基因的序列公布于Wehrmann,A.等(1998)J.Bacteriol.180(12):3159-3165中。然而,本申请发明人获得的序列显著比所公布的形式长。认为所公布的形式依赖于不正确的起始密码子,因此仅代表真实编码区的一个片段。
在另一优选实施方案中,本发明的分离的核酸分子包含作为本发明核苷酸序列(例如序列表中的奇数SEQ ID NO序列)之一的互补序列的核酸分子或其部分。作为本发明中核苷酸序列之一的互补序列的核酸分子是与序列表中所示的核苷酸序列(例如奇数SEQ ID NO序列)之一足够互补、使得可以与本发明核苷酸序列之一杂交并因此形成稳定双链体的核酸分子。
在再一优选实施方案中,本发明的分离的核酸分子包含这样的核苷酸序列或其部分,所述核苷酸序列与本发明核苷酸序列(例如序列表中的奇数SEQ ID NO序列)的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,将包括采用上述值作为上限和/或下限的任何组合的标识值范围。在另一优选实施方案中,本发明的分离的核酸分子包含与本发明核苷酸序列之一杂交、例如在严格条件下杂交的核苷酸序列或其部分。
此外,本发明的核酸分子可以仅包含序列表中一种奇数SEQ IDNO序列编码区的一部分,例如可以用作探针或引物的片段或编码HA蛋白的生物活性部分的片段。根据从谷氨酸棒杆菌克隆HA基因而测定的核苷酸序列,使得可以产生设计用于鉴定和/或克隆其它细胞类型和生物中的HA同系物以及来自其它棒杆菌或相关菌种的HA同系物的探针和引物。所述探针/引物通常包含基本上纯化的寡核苷酸。所述寡核苷酸通常包含一种核苷酸序列区,所述核苷酸序列区在严格条件下与本发明核苷酸序列之一(例如序列表中一种奇数SEQ ID NO序列)的有义链、这些序列之一的反义序列或其天然存在的突变体的至少约12个、优选约25个、更优选约40个、50个或75个连续核苷酸杂交。基于本发明核苷酸序列的引物可以用于PCR反应中,以克隆HA同系物。基于所述HA核苷酸序列的探针可以用来检测编码同一蛋白或同源蛋白的转录物或基因组序列。在优选实施方案中,所述探针还包含与其连接的一个标记基团,例如所述标记基团可以是放射性同位素、荧光化合物、酶或酶辅因子。这类探针可以用作诊断试验试剂盒的一部分,以鉴定错误表达(misexpress)HA蛋白的细胞,例如通过测定细胞样品中HA编码核酸的水平,例如检测HA mRNA水平或检测基因组HA基因是否已经突变或缺失。
在一个实施方案中,本发明的核酸分子编码一种包含与本发明氨基酸序列(例如序列表中偶数SEQ ID NO序列)足够同源的氨基酸序列的蛋白质或其部分,使得所述蛋白质或其部分保留参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能的能力。本文所用的用语“足够同源”是指这样的蛋白质或其部分,所述蛋白质或其部分所具有的氨基酸序列包含最小数目的与本发明氨基酸序列相同或等同(例如所具有的侧链与序列表中一种偶数SEQ ID NO序列中的氨基酸残基相似的氨基酸残基)氨基酸残基,使得所述蛋白质或其部分能够参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能。参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种本文所述的谷氨酸棒杆菌酶活性或蛋白水解活性的蛋白质,在一种或多种精细化学品的生产和分泌方面起作用。在本文中也描述了这类活性的实例。因此,“HA蛋白的功能”或者直接或者间接地影响一种或多种精细化学品生产的收率、产量和/或产率。HA蛋白活性的实例示于表1中。
在另一实施方案中,所述蛋白质与本发明的完整氨基酸序列(例如序列表偶数SEQ ID NO序列)的同源性为至少约50-60%,优选至少约60-70%,更优选至少约70-80%、80-90%、90-95%,最优选至少约96%、97%、98%、99%或更高。
本发明HA核酸分子所编码的蛋白质的部分最好是一种所述HA蛋白的生物活性部分。本文所用的术语“HA蛋白的生物活性部分”将包括可以参与维持谷氨酸棒杆菌内稳态或可以执行参与该微生物对不同环境条件适应的功能的HA蛋白的部分,例如结构域/基序,或具有表1中所述的一种活性。为了确定HA蛋白或其生物活性部分是否可以参与谷氨酸棒杆菌细胞壁生物合成或重排、无机化合物的代谢、芳族或脂族化合物的修饰或降解、或具有一种谷氨酸棒杆菌酶活性或蛋白水解活性,可以进行酶活性的测定。这类测定方法是本领域技术人员众所周知的,如实施例部分的实施例8中详细描述的。
通过分离一种本发明氨基酸序列(例如序列表中偶数SEQ ID NO序列)的一部分,表达所编码的所述HA蛋白或肽的一部分(例如通过体外重组表达),并且评价所编码的所述HA蛋白或肽部分的活性,可以制备另外的编码HA蛋白生物活性部分的核酸片段。
本发明还包括由于遗传密码的简并性而不同于本发明核苷酸序列(例如序列表中的奇数SEQ ID NO序列)之一(和其部分)并因此编码与本发明核苷酸序列所编码蛋白相同的HA蛋白的核酸分子。在另一实施方案中,本发明的分离的核酸分子所具有的核苷酸序列编码具有序列表中所示氨基酸序列(例如偶数SEQ ID NO)的蛋白质。在再一实施方案中,本发明的核酸分子编码与本发明氨基酸序列(由序列表中奇数SEQ ID NO中所示的可读框编码)基本上同源的全长谷氨酸棒杆菌蛋白。
本领域技术人员会理解,在一个实施方案中,本发明的序列不意味着包括现有技术的序列,例如表2或4中叙述的在本发明之前获得的Genbank序列的那些序列。在一个实施方案中,本发明包括与本发明的核苷酸序列或氨基酸序列具有的同一性百分比大于现有技术序列(例如表2或4中叙述的Genbank序列(或由这种序列编码的蛋白质))的同一性百分比的核苷酸序列和氨基酸序列。例如,本发明包括:与名为RXA00471(SEQ ID NO:293)的核苷酸序列的同一性大于39%和/或至少为39%的核苷酸序列;与名为RXA00500(SEQ ID NO:143)的核苷酸序列的同一性大于41%和/或至少为41%的核苷酸序列;以及与名为RXA00502(SEQ ID NO:147)的核苷酸序列的同一性大于35%和/或至少为35%的核苷酸序列。本领域技术人员通过检查任何给定的本发明序列三个最高命中各自的表4中所示GAP计算的同一性百分比分值,并且从100%中减去GAP计算的最高同一性百分比分值,能够计算出所述给定的本发明序列同一性百分比的下限。本领域技术人员也会认识到,本发明也包括同一性百分比大于如此计算的下限(例如同一性至少50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高)的核酸序列和氨基酸序列。
除了序列表中奇数SEQ ID NO中所示的谷氨酸棒杆菌HA核苷酸外,本领域技术人员会认识到,在群体(例如谷氨酸棒杆菌群体)中可能存在导致HA蛋白氨基酸序列改变的DNA序列多态性。由于自然变异,这类所述HA基因中的遗传多态性可能存在于群体的个体中。本文所用的术语“基因”和“重组基因”是指包含编码HA蛋白、最好是谷氨酸棒杆菌HA蛋白的可读框的核酸分子。这类天然变异通常可以导致所述HA基因核苷酸序列中1-5%的变异。由于自然变异的结果并且不改变HA蛋白功能活性的HA中的任何和所有这类核苷酸变异和所产生的氨基酸多态性将包括在本发明范围内。
根据对应于本发明谷氨酸棒杆菌HA DNA的天然变异体和非谷氨酸棒杆菌同系物的核酸分子与本文所公开的谷氨酸棒杆菌HA核酸的同源性,采用所述谷氨酸棒杆菌DNA或其部分作为严格杂交条件下按照标准杂交技术的杂交探针,可以分离出所述核酸分子。因此,在另一实施方案中,本发明的分离的核酸分子至少长15个核苷酸,并且在严格条件下与包含序列表中奇数SEQ ID NO核苷酸序列的核酸分子杂交。在其它实施方案中,所述核酸至少长30个、50个、100个、250个或更多个核苷酸。本文所用的术语“在严格条件下杂交”意在描述相互之间至少60%同源的核苷酸序列通常保留相互杂交的杂交和洗涤条件。最好是,所述条件使得相互之间同源性为至少约65%、更优选至少约70%、甚至更优选至少约75%或更高的序列通常保持相互杂交。这类严格条件是本领域技术人员已知的,并且可以在CurrentProtocols in Molecular Biology,John Wiley & Sons,N.Y.(1989),6.3.1-6.3.6中找到。严格杂交条件的一个优选的非限制性实例是在6X氯化钠/柠檬酸钠(SSC)中于约45℃杂交,然后在0.2XSSC,0.1%SDS中于50-65℃进行一次或多次洗涤。最好是,在严格条件下与本发明核苷酸序列杂交的本发明分离的核酸分子对应于天然存在的核酸分子。本文所用的“天然存在的”核酸分子是指具有天然存在的核苷酸序列(例如编码天然蛋白质)的RNA或DNA分子。在一个实施方案中,所述核酸编码一种天然谷氨酸棒杆菌HA蛋白。
除了在群体中可能存在的所述HA序列的天然存在的变异体外,本领域技术人员还会认识到,可以通过在本发明核苷酸序列中进行突变而导入改变,由此导致所编码HA蛋白氨基酸序列的改变,而不改变所述HA蛋白的功能能力。例如,可以在本发明核苷酸中进行导致在“非必需”氨基酸残基上氨基酸取代的核苷酸取代。“非必需”氨基酸残基是可以在所述HA蛋白之一的野生型序列(例如序列表的偶数SEQ ID NO)中被改变、而不改变所述HA蛋白活性的残基,而“必需”氨基酸残基是HA蛋白活性所需的。然而,其它氨基酸残基(例如在具有HA活性的结构域中不保守或仅半保守的那些残基)可能不是活性必需的,因此可能适合改变而不改变HA活性。
因此,本发明的另一方面涉及编码HA蛋白、含有HA活性非必需的氨基酸残基改变的核酸分子。这类HA蛋白在氨基酸序列上不同于序列表中偶数SEQ ID NO的序列,但仍保留至少一种本文所述的HA活性。在一个实施方案中,所述分离的核酸分子包含编码蛋白质的核苷酸序列,其中所述蛋白质包含与本发明氨基酸序列至少约50%同源的氨基酸序列,并且能够参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能,或具有表1中所述的一种或多种活性。最好是,所述核酸分子编码的蛋白质与序列表中一种奇数SEQ ID NO的氨基酸序列至少约50-60%同源,更优选与这些序列之一至少约60-70%同源,甚至更优选与这些序列之一至少约70-80%、80-90%、90-95%同源,最优选与一种本发明的氨基酸序列至少约96%、97%、98%或99%同源。
为了确定两种氨基酸序列(例如一种本发明的氨基酸序列和其突变体形式)或两种核酸的同源性百分比,将所述序列进行序列比对,以进行最佳比较(例如可以在一种蛋白质或核酸的序列引入空位,以与另一种蛋白质或核酸进行最佳序列比对)。然后比较对应氨基酸位置或核苷酸位置上的氨基酸残基或核苷酸。当一个序列(例如一种本发明的氨基酸序列)中的一个位置被另一个序列(例如所述氨基酸序列的突变体形式)中相应位置的同一氨基酸残基或核苷酸占据时,则所述两种分子在该位置是同源的(即本文所用的氨基酸或核酸“同源性”等同于氨基酸或核酸“同一性”)。这两个序列之间的同源性百分比随所述序列共享的相同位置数而变化(即同源性百分比=相同位置数/位置总数×100)。
编码本发明蛋白序列(例如序列表中偶数SEQ ID NO序列)同源的HA蛋白的分离的核酸分子可以通过以下步骤构建:在本发明的核苷酸序列中,导入一个或多个核苷酸取代、添加或缺失,使得在所编码的蛋白质中导入一个或多个氨基酸取代、添加或缺失。可以采用标准技术,例如定点诱变和PCR介导的诱变,将突变导入一种本发明的核苷酸序列中。最好是,在一个或多个预测的非必需氨基酸残基上进行保守氨基酸取代。“保守氨基酸取代”是其中所述氨基酸残基被具有相似侧链的一个氨基酸残基取代的氨基酸取代。在本领域中已经确定了具有相似侧链的氨基酸残基类别。这些类别包括具有碱性侧链的氨基酸(例如赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如苏氨酸、缬氨酸、异亮氨酸)和具有芳族侧链的氨基酸(例如酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此,HA蛋白中预测的非必需氨基酸残基最好被来自同一侧链类别的另一种氨基酸残基取代。或者,在另一实施方案中,可以沿全部或部分HA编码序列随机导入突变,例如通过饱和诱变导入突变,并且可以根据本文所述的HA活性对所产生的突变体进行筛选,以鉴定保留HA活性的突变体。对序列表中奇数SEQ ID NO之一的核苷酸序列诱变后,可以重组表达所编码的蛋白质,然后可以采用例如本文所述的测定(参见实施例部分的实施例8)测定所述蛋白质的活性。
除了上述编码HA蛋白的核酸分子外,本发明的另一方面涉及反义的分离的核酸分子。“反义”核酸包括与编码蛋白质的“有义”核酸互补的核苷酸序列,例如与双链DNA分子的编码链互补或与mRNA序列互补的核苷酸序列。因此,反义核酸可以与有义核酸形成氢键。所述反义核酸与完整的HA编码链或仅与其部分互补。在一个实施方案中,反义核酸分子是编码HA蛋白的核苷酸序列编码链的“编码区”的反义核酸分子。术语“编码区”是指包含被翻译为氨基酸残基的密码子的核苷酸序列区(例如SEQ ID NO:3(RXN00249)的完整编码区包含核苷酸1-957)。在另一实施方案中,所述反义核酸分子是编码HA的核苷酸序列编码链“非编码区”的反义核酸分子。术语“非编码区”是指邻接所述编码区、不被翻译为氨基酸的5’序列和3’序列(即也称为5’和3’非翻译区)。
已知本文公开的编码HA的编码链序列(例如序列表中奇数SEQID NO中所示的序列),因此可以依照Waston和Crick碱基配对原则,设计本发明的反义核酸。所述反义核酸分子可以与HA mRNA的完整编码区互补,但更优选是作为仅HA mRNA编码区或非编码区一部分的反义分子的寡核苷酸。例如,所述反义寡核苷酸可以与HA mRNA翻译起始位点周围的区域互补。所述反义寡核苷酸可以例如长约5个、10个、15个、20个、25个、30个、35个、40个、45个或50个核苷酸。可以采用化学合成以及采用本领域已知的方法的酶连接反应,构建本发明的反义核酸。例如,可以用天然存在的核苷酸或设计用以增加所述分子生物学稳定性或增加所述反义核酸和有义核酸之间所形成的双链体物理稳定性的各种修饰的核苷酸,化学合成反义核酸(例如反义寡核苷酸),例如可以使用硫代磷酸衍生物以及吖啶取代的核苷酸。可以用来产生所述反义核酸的经修饰的核苷酸的实例包括5-氟尿嘧啶、5-溴尿嘧啶、5-氯尿嘧啶、5-碘尿嘧啶、次黄嘌呤、黄嘌呤、4-乙酰胞嘧啶、5-(羧基羟甲基)尿嘧啶、5-羧甲基氨基甲基-2-硫代尿苷、5-羧甲基氨基甲基尿嘧啶、二氢尿嘧啶、β-D-半乳糖基Q核苷(queosine)、肌苷、N6-异戊烯基腺嘌呤、1-甲基鸟嘌呤、1-甲基肌苷、2,2-二甲基鸟嘌呤、2-甲基腺嘌呤、2-甲基鸟嘌呤、3-甲基胞嘧啶、5-甲基胞嘧啶、N6-腺嘌呤、7-甲基鸟嘌呤、5-甲基氨基甲基尿嘧啶、5-甲氧基氨基甲基-2-硫尿嘧啶、β-D-甘露糖基Q核苷、5’-甲氧基羧基甲基尿嘧啶、5-甲氧基尿嘧啶、2-甲硫基-N6-异戊烯基腺嘌呤、尿嘧啶-5-羟基乙酸(v)、wybutoxosine、假尿嘧啶、Q核苷、2-硫代胞嘧啶、5-甲基-2-硫尿嘧啶、2-硫尿嘧啶、4-硫尿嘧啶、5-甲基尿嘧啶、尿嘧啶-5-羟基乙酸甲酯、尿嘧啶-5-羟基乙酸(v)、5-甲基-2-硫尿嘧啶、3-(3-氨基-3-N-2-羧丙基)尿嘧啶、(acp3)w和2,6-二氨基嘌呤。另一方面,可以采用已经以反义方向亚克隆了一种核酸(即由所插入的核酸转录出的RNA将具有所感兴趣的靶核酸的反义方向,在以下小节中进一步描述)的表达载体,用生物学方法产生所述反义核酸。
通常将本发明的反义核酸分子给予细胞或使其原位产生,使得它们与编码HA蛋白的细胞mRNA和/或基因组DNA杂交或结合,以由此抑制所述蛋白的表达,例如通过抑制转录和/或翻译抑制表达。可以借助常规的核苷酸互补性进行杂交,以形成稳定的双链体,或例如在与DNA双链体结合的反义核酸分子的情况下,通过双螺旋主沟中的特定相互作用,而进行杂交。可以对所述反义分子进行修饰,使得它例如通过使所述反义核酸分子与结合于细胞表面受体或抗原的肽或抗体连接,而特异性地结合于所选定细胞表面表达的受体或抗原。也可以用本文描述的载体将所述反义核酸分子传递至细胞。为了获得足够胞内浓度的所述反义分子,优选其中所述反义核酸分子置于强原核生物、病毒或真核生物启动子控制之下的载体构建体。
在再一实施方案中,本发明的反义核酸分子是α-异头核酸分子。α-异头核酸分子与互补RNA形成特定的双链杂交体,其中与通常的β单位相反,所述链互补平行(Gaultier等(1987)Nucleic Acids.Res.15:6625-6641)。所述反义核酸分子也可以包含2’-o-甲基核糖核苷酸(Inoue等(1987)Nucleic Acids Res.15:6131-6148)或嵌合RNA-DNA类似物(Inoue等(1987)FEBS Lett.215:327-330)。
在又一实施方案中,本发明的反义核酸是一种核酶。核酶是具有核糖核酸酶活性的催化性RNA分子,它们能够切割与其具有互补区的单链核酸,例如mRNA。因此,可以用核酶(例如锤头核酶(描述于Haselhoff和Gerlach(1988)Nature 334:585-591)来催化切割HA mRNA转录物,由此抑制HA mRNA的翻译。可以根据本文公开的HA DNA分子的核苷酸序列(即SEQ ID NO.3(RXN00249)),设计对HA编码核酸具有特异性的核酶。例如,可以构建一种四膜虫属(Tetrahymena)L-19 IVS RNA的衍生物,其中活性部位的核苷酸序列与HA编码mRNA中待切割的核苷酸序列互补。参见例如Cech等的美国专利号4,987,071和Cech等的美国专利号5,116,742。或者,可以用HA mRNA,从RNA分子库中选择具有特定核糖核酸酶活性的催化性RNA。参见例如Bartel,D.和Szostak,J.W.(1993)Science 261:1411-1418。
另一方面,通过靶向与HA核苷酸序列调节区(例如HA启动子和/或增强子)互补的核苷酸序列,以形成阻止靶细胞中HA基因转录的三螺旋结构,可以抑制HA基因的表达。一般参见Helene,C.(1991)Anticancer Drug Des.6(6):569-84;Helene,C.等(1992)Ann.N.Y.Acad.Sci.660:27-36;和Maher,L.J.(1992)Bioassays 14(12):807-15。B.重组表达载体和宿主细胞
本发明的另一方面涉及含编码HA蛋白(或其部分)的核酸的载体,优选表达载体。本文所用的术语“载体”是指能够转运与其连接的另一种核酸的核酸分子。一种类型的载体是“质粒”,质粒是指其中可以连接另外的DNA区段的环状双链DNA环。另一种类型的载体是病毒载体,其中可以将另外的DNA区段连接到病毒基因组中。某些载体能够在它们所导入的宿主细胞中自主复制(例如具有细菌复制起点的细菌载体和附加型哺乳动物载体)。其它载体(例如非附加型哺乳动物载体)在导入到宿主细胞中后整合到宿主细胞的基因组中,由此与宿主基因组一起复制。此外,某些载体能够指导与其有效连接的基因的表达。这类载体在本文中称为“表达载体”。一般而言,在重组DNA技术中使用的表达载体通常是质粒形式的载体。在本说明书中,“质粒”和“载体”可以互换使用,因为质粒是最常使用的载体形式。然而,本发明将包括这类其它形式的表达载体,例如病毒载体(例如复制缺陷型反转录病毒、腺病毒和腺相关病毒),它们发挥等同的功能。
本发明的重组表达载体包含适合于在宿主细胞中表达本发明核酸的形式的本发明核酸,这是指所述重组表达载体包括根据用于表达的宿主细胞选择的一个或多个调节序列,所述调节序列有效连接于待表达的核酸序列。在重组表达载体中,“有效连接的”是指将所感兴趣的核苷酸序列以允许所述核苷酸序列表达的方式(例如在体外转录/翻译系统中或当将所述载体导入宿主细胞时在所述宿主细胞中)连接于所述调节序列。术语“调节序列”将包括启动子、增强子和其它表达控制元件(例如聚腺苷酸化信号)。这类调节序列描述于例如Goeddel;Gene Expression Technology: Methods in Enzymology 185,AcademicPress,San Diego,CA(1990)。调节序列包括在许多类型宿主细胞中指导核苷酸序列组成型表达的那些调节序列、以及在某些宿主细胞中指导所述核苷酸序列表达的那些调节序列。优选的调节序列是例如启动子,例如cos-、tac-、trp-、tet-、trp-tet、lpp-、lac-、lpp-lac、lacIq-、T7-、T5-、T3-、gal-、trc-、ara-、SP6-、amy、SPO2、λ-PR-或λPL,它们优选用于细菌中。其它调节序列是例如来自酵母和真菌的启动子,例如ADCl、MFα、AC、P-60、CYCl、GAPDH、TEF、rp28、ADH;来自植物的启动子,例如CaMV/35S、SSU、OCS、lib4、usp、STLS1、B33、nos或遍在蛋白启动子或菜豆蛋白启动子。也有可能使用人工启动子。本领域技术人员会认识到,所述表达载体的设计可取决于诸如待转化的宿主细胞的选择、所需蛋白的表达水平等的因素。可以将本发明的表达载体导入宿主细胞中,由此产生由本文所述的核酸编码的蛋白质或肽,包括融合蛋白或肽(例如HA蛋白、突变形式的HA蛋白、融合蛋白等)。
可以设计本发明的重组表达载体用于在原核细胞或真核细胞中表达HA蛋白。例如,可以在细菌细胞例如谷氨酸棒杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母和其它真菌细胞(参见Romanos,M.A.等(1992)“酵母中的外源基因表达:综述”,Yeast 8:423-488;van den Hondel,C.A.M.J.J.等(1991)“丝状真菌中的异源基因表达”,载于:More GeneManipulations in Fungi,J.W.Bennet和L.L.Lasure编著,第396-428页:Academic Press:San Diego;和van den Hondel,C.A.M.J.J.和Punt,P.J.(1991),“用于丝状真菌的基因转移系统和载体开发”,载于:AppliedMolecular Genetics of Fungi,Peberdy,J.F.等编著,第1-28页,CambridgeUniversity Press:Cambridge)、藻类和多细胞植物细胞(参见Schmidt,R.和Willmitzer,L.(1988)“高效根癌农杆菌(Agrobacterium tumefaciens)介导的拟南芥(Arabidopsis thaliana)和子叶外植体的转化”Plant CellRep.:583-586)或哺乳动物细胞中,表达HA基因。合适的宿主细胞在Goeddel,Gene Expression Technology:Methods in Enzymology 185,Academic Press,San Diego,CA(1990)中有进一步的描述。或者,所述重组表达载体可以在体外例如用T7启动子调节序列和T7聚合酶进行转录和翻译。
原核生物中的蛋白表达最常用含有指导或者融合蛋白或者非融合蛋白表达的组成型或诱导型启动子的载体来进行。融合载体将多个氨基酸加至其中所编码蛋白上,通常加至所述重组蛋白的氨基末端,但也加至C末端,或在所述蛋白质中的合适区中融合。这类融合载体通常用于三个目的:1)为了增加重组蛋白的表达;2)为了增加所述重组蛋白的溶解性;和3)为了通过用作亲和纯化中的配体而有助于所述重组蛋白的纯化。通常,在融合表达载体中,在融合部分和重组蛋白的接点处引入一个蛋白酶切割位点,以使得能够将重组蛋白与融合部分分开,以随后纯化所述融合蛋白。这类酶及其相关的识别序列包括因子Xa、凝血酶和肠激酶。
典型的融合表达载体包括pGEX(Pharmacia Biotech Inc;Smith,D.B.和Johnson,K.S.(1988)Gene 67:31-40)、pMAL(New EnglandBiolabs,Beverly,MA)和pRIT5(Pharmacia,Piscataway,NJ),它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白或A蛋白与靶重组蛋白融合。在一个实施方案中,将所述HA蛋白的编码序列克隆到pGEX表达载体中,以产生编码融合蛋白的载体,所述融合蛋白从N末端至C末端包含GST-凝血酶切割位点-X蛋白。所述融和蛋白可以用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。不与GST融合的重组HA蛋白可以通过用凝血酶切割所述融合蛋白进行回收。
合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等,(1988)Gene 69:301-315)、pLG338、pACYC184、pBR322、pUC18、pUC19,pKC30、pRep4、pHS1、pHS2、pPLc236、pMBL24、pLG200、pUR290、pIN-III113-B1、λgt11、pBdC1和pET 11d(Studier等,GeneExpression Technology:Methods in Enzymology 185,Academic Press,SanDiego,California(1990)60-89;和Pouwels等编著(1985)Cloning Vectors.Elsevier:New York IBSN 0 444 904018)。来自pTrc载体的靶基因的表达依赖于来自杂种trp-lac融合启动子的宿主RNA聚合酶转录。来自pET11d载体的靶基因的表达依赖于通过共同表达的病毒RNA聚合酶(T7 gn1)介导的T7 gn10-lac融合启动子的转录。这种病毒聚合酶由来自含有lacUV5启动子转录控制下的T7 gn1基因的居留(resident)λ原噬菌体的宿主菌株BL21(DE3)或HMS174(DE3)供应。关于其它细菌变种的转化,可以选择合适的载体。例如,已知质粒pIJ01、pIJ364、pIJ702和pIJ361可用于转化链霉菌属(Streptomyces),质粒pUB110、pC194或pBD214适用于转化芽孢杆菌属(Bacillus)菌种。用于将遗传信息转移到棒杆菌属的几种质粒包括pHM1519、pBL1、pSA77或pAJ667(Pouwels等编著(1985)Cloning Vectors.Elsevier:New York IBSN 0 444904018)。
使重组蛋白表达最大化的一种策略是在蛋白酶剪切所述重组蛋白能力受损的宿主细菌中表达所述蛋白(Gottesman,S.,GeneExpression Technology:Methods in Enzymology 185,Academic Press,SanDiego,California(1990)119-128)。另一种策略是改变待插入到表达载体中的核酸的核酸序列,使得每个氨基酸的各个密码子是在选定用于表达的细菌(例如谷氨酸棒杆菌)中优先使用的密码子(Wada等(1992)Nucleic Acids Res.20:2111-2118)。这种本发明核酸序列的改变可以用标准DNA合成技术来进行。
在另一实施方案中,所述HA蛋白表达载体是一种酵母表达载体。用于在酵母酿酒酵母(S.cerevisiae)中表达的载体的实例包括pYepSecl(Baldari等(1987)Embo J.6:229-234)、2μ、pAG-1、Yep6、Yep13、pEMBLYe23、pMFa(Kurjan和Herskowitz(1982)Cell 30:933-943)、pJRY88(Schultz等,(1987)Gene 54:113-123)和pYES2(InvitrogenCorporation,San Diego,CA)。适用于其它真菌例如丝状真菌的载体以及载体的构建方法包括在以下文献中详述的载体和方法:van denHondel,C.A.M.J.J.和Punt,P.J.(1991)“用于丝状真菌的基因转移系统和载体开发”,载于:Applied Molecular Genetics of Fungi,J.F.Peberdy等编著,第1-28页,Cambridge University Press:Cambridge,和Pouwels等编著(1985)Cloning Vectors.Elsevier:New York(IBSN 0 444904018)。
另一方面,本发明的HA蛋白可以用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf9细胞)中表达蛋白的杆状病毒载体包括pAc系列(Smith等(1983)Mol.Cell Biol.3:2156-2165)和pVL系列(Lucklow和Summers(1989)Virology 170:31-39)。
在另一实施方案中,本发明的HA蛋白可以在单细胞植物细胞(例如藻类)或在来自高等植物(例如种子植物,诸如作物)的植物细胞中表达。植物表达载体的实例包括详述于以下文献中的表达载体:Becker,D.,Kemper,E.,Schell,J.和Masterson,R.(1992)“具有位于左边界邻近的选择标记的新型植物双元载体”,Plant Mol.Biol.20:1195-1197;和Bevan,M.W.(1984)“用于植物转化的农杆菌双元载体”,Nucl.Acid.Res.12:8711-8721,并且包括pLGV23、pGHlac+、pBIN19、pAK2004和pDH51(Pouwels等编著(1985)Cloning Vectors.Elsevier:New YorkIBSN 0 444 904018)。
在再一实施方案中,本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed,B.(1987)Nature 329:840)和pMT2PC(Kaufman等(1987)EMBO J.6:187-195)。当用于哺乳动物细胞中时,所述表达载体的控制功能通常由病毒调节元件提供。例如,常用的启动子得自多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。对于用于原核细胞和真核细胞的其它合适的表达系统,参见Sambrook,J.,Fritsh,E.F.和Maniatis,T.Molecular Cloning:ALaboratory Manual.第2版的第16和17章,Cold Spring HarborLaboratory,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,1989。
在另一实施方案中,哺乳动物重组表达载体能够指导所述核酸优先在特定的细胞类型中表达(例如用组织特异性调节元件来表达所述核酸)。组织特异性调节元件是本领域已知的。合适的组织特异性启动子的非限制性实例包括清蛋白启动子(肝特异性;Pinkert等(1987)Genes Dev.1:268-277)、淋巴(lymphoid)特异性启动子(Calame和Eaton(1988)Adv.Immunol.43:235-275)、特别是T细胞受体的启动子(Winoto和Baltimore(1989)EMBO J.8:729-733)和免疫球蛋白的启动子(Banerji等(1983)Cell 33:729-740;Queen和Baltimore(1983)Cell 33:741-748)、神经元特异性启动子(例如神经丝启动子;Byrne和Ruddle(1989)PNAS86:5473-5477)、胰腺特异性启动子(Edlund等(1985)Science 230:912-916)和乳腺特异性启动子(例如乳清启动子;美国专利号4,873,316和欧洲申请公布号264,166)。也包括发育调节型启动子,例如鼠类hox启动子(Kessel和Gruss(1990)Science 249:374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)Genes Dev.3:537-546)。
本发明还提供包含以反义方向克隆到表达载体中的本发明DNA分子的重组表达载体。亦即所述DNA分子与调节序列有效地连接,其连接方式提供HA mRNA的反义RNA分子的表达(通过转录所述DNA分子)。可以选择有效连接于反义方向克隆的核酸的、指导在多种细胞类型中连续表达所述反义RNA分子的调节序列,例如病毒启动子和/或增强子,或者可以选择指导反义RNA的组成型、组织特异性或细胞类型特异性表达的调节序列。反义表达载体可以为重组质粒、噬菌粒或减毒病毒的形式,其中反义核酸在高效调节区的控制之下产生,其活性可以由所述载体所导入的细胞类型决定。对于使用反义基因的基因表达调节的讨论,参见Weintraub,H等,作为遗传分析分子工具的反义RNA,Reviews-Trends in Genetics,第1(1)卷1986。
本发明的另一方面涉及已经导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在本文中可互换使用。不言而喻,这种术语不仅是指特定的题述细胞,而且也指这种细胞的子代或潜在的子代。因为在连续世代中由于或者突变或者环境影响而可能存在某些修饰,所以这类子代事实上可能与亲代细胞不完全相同,但仍包括在本文所述的术语范围内。
宿主细胞可以是任何原核细胞或真核细胞。例如,HA蛋白可以在细菌细胞例如谷氨酸棒杆菌、昆虫细胞、酵母细胞或哺乳动物细胞(例如中国仓鼠卵巢细胞(CHO)或COS细胞)中表达。其它合适的宿主细胞是本领域技术人员已知的。与谷氨酸棒杆菌相关的、可以方便地用作本发明核酸和蛋白分子的宿主细胞的微生物示于表3中。
可以通过常规转化或转染技术,将载体DNA导入原核细胞或真核细胞中。本文所用的术语“转化”和“转染”、“接合”和“转导”是指本领域公知的用于将外源核酸(例如,线性DNA或RNA(例如线性化载体或无载体的单独的基因构建体))或载体形式(例如质粒、噬菌体、噬菌粒(phasmid)、噬菌粒(phagemid)、转座子或其它DNA)的核酸导入宿主细胞的多种技术,包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖介导的转染、脂转染、天然感受态、化学物质介导的转移或电穿孔。用于转化或转染宿主细胞的合适方法可以在Sambrook等(MolecularCloning:A Laboratory Manual.第2版,Cold Spring Harbor Laboratory,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,1989)和其它实验指南中找到。
关于哺乳动物细胞的稳定转染,已知根据所用的表达载体和转染技术,仅一小部分细胞可以将外源DNA整合到其基因组中。为了鉴定和选择这些整合子,通常将编码选择标记(例如抗生素抗性)的基因与所感兴趣的基因一起导入宿主细胞中。优选的选择标记包括赋予对药物例如G418、潮霉素和氨甲蝶呤的抗性的那些选择标记。可以将编码选择标记的核酸在编码HA蛋白的同一载体上导入宿主细胞,或者可以将其在单独的载体上导入。用所导入核酸稳定转染的细胞可以通过例如药物选择进行鉴定(例如已经掺入选择标记基因的细胞将存活,而其它细胞死亡)。
为了产生同源重组微生物,制备含有其它已经引入缺失、添加或取代以由此改变(例如功能性破坏)所述HA基因的至少一部分HA基因的载体。最好是,该HA基因是谷氨酸棒杆菌HA基因,但它可以是来自相关细菌或甚至来自哺乳动物、酵母或昆虫来源的同系物。在一个优选实施方案中,设计所述载体,使得在同源重组后,所述内源HA基因被功能性破坏(即不再编码功能性蛋白;也称为“剔除”载体)。或者,可以设计所述载体,使得在同源重组后,所述内源HA基因被突变或者被改变,但仍编码功能性蛋白(例如可以改变上游调节区,以由此改变所述内源HA蛋白的表达)。在所述同源重组载体中,所述HA基因的被改变的部分在其5’端和3’端邻接另一种HA基因的核酸,使得能够在所述载体所携带的外源HA基因和微生物中的内源HA基因之间发生同源重组。所述另一种侧翼HA核酸具有足以与内源基因成功同源重组的长度。通常,在所述载体中包含数千碱基的侧翼DNA(5’端以及3’端)(有关同源重组载体的描述,参见例如Thomas,K.R.和Capecchi,M.R.(1987)Cell 51:503)。将所述载体导入微生物中(例如通过电穿孔),然后用本领域已知的技术选择其中所导入的HA基因与内源HA基因同源重组过的细胞。
在另一实施方案中,可以产生含有选定系统的重组微生物,所述选定系统提供受调节的所导入基因的表达。例如,在载体上包含在置于lac操纵子控制之下的HA基因,允许所述HA基因仅在IPTG存在时表达。这类调节系统是本领域众所周知的。
在另一实施方案中,破坏宿主细胞中的内源HA基因(例如通过同源重组或本领域已知的其它遗传方法),使得不发生其蛋白产物的表达。在另一实施方案中,通过一个或多个点突变、缺失或倒位,已经改变了宿主细胞中内源的或所导入的HA基因,但它仍编码功能性HA蛋白。在再一实施方案中,改变了微生物中HA基因的一个或多个调节区(例如启动子、阻抑蛋白或诱导物)(例如通过缺失、截短、倒位或点突变),使得调节所述HA基因的表达。本领域技术人员会认识到,含有一种以上的所述HA基因和蛋白修饰的宿主细胞可以采用本发明的方法容易地产生,并且意味着将包括在本发明内。
本发明的宿主细胞(例如培养物中的原核宿主细胞或真核宿主细胞)可以用来产生(即表达)HA蛋白。因此,本发明还提供使用本发明的宿主细胞生产HA蛋白的方法。在一个实施方案中,所述方法包括在合适的培养基中培养本发明的宿主细胞(其中已经导入了编码HA蛋白的重组表达载体,或其中基因组已经导入了编码野生型的或改变的HA蛋白的基因),直至产生HA蛋白。在另一实施方案中,所述方法还包括从培养基中或从宿主细胞中分离HA蛋白。C.分离的HA蛋白
本发明的另一方面涉及分离的HA蛋白及其生物活性部分。“分离的”或“纯化的”蛋白或其生物活性部分当通过重组DNA技术产生时基本上不含细胞物质,或在化学合成时基本上不含化学前体或其它化学物质。用语“基本上不含细胞物质”包括其中所述蛋白质与细胞中天然产生或重组产生的细胞组分分离的HA蛋白制备物。在一个实施方案中,用语“基本上不含细胞物质”包括所具有非HA蛋白(本文中也称为“污染蛋白”)低于约30%(以干重计)、更优选低于20%、再更优选低于约10%、最优选低于约5%的HA蛋白制备物。当HA蛋白或其生物活性部分重组产生时,也优选基本上不含培养基,即培养基低于所述蛋白制备物体积的约20%,更优选低于约10%,最优选低于约5%。用语“基本上不含化学前体或其它化学物质”包括其中HA蛋白与参与所述蛋白合成的化学前体或其它化学物质分离的HA蛋白制备物。在一个实施方案中,用语“基本上不含化学前体或其它化学物质”包括所具有的化学前体或非HA化学物质低于约30%(以干重计)、更优选低于20%、再更优选低于约10%、最优选低于约5%的HA蛋白制备物。在优选实施方案中,分离的蛋白质或其生物活性部分没有来自所述HA蛋白所来源的同一生物的污染蛋白。通常,这类蛋白通过在微生物例如谷氨酸棒杆菌中重组表达例如谷氨酸棒杆菌HA蛋白来产生。
本发明的分离的HA蛋白或其部分可以参与谷氨酸棒杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌,或具有表1中所述的一种或多种活性。在优选实施方案中,所述蛋白质或其部分包含与一种本发明氨基酸序列(例如序列表中一种偶数SEQ ID NO序列)足够同源的氨基酸序列,使得所述蛋白质或其部分保留参与维持谷氨酸棒杆菌内稳态或执行参与该微生物对不同环境条件适应的功能的能力。所述蛋白的所述部分最好是本文所述的生物活性部分。在另一优选实施方案中,本发明的HA蛋白具有序列表中一个偶数SEQ ID NO中所示的氨基酸序列。在再一优选实施方案中,所述HA蛋白具有与一种本发明核苷酸序列(例如序列表中的奇数SEQ ID NO序列)杂交(例如在严格条件下杂交)的核苷酸序列所编码的氨基酸序列。在又一优选实施方案中,所述HA蛋白具有由下述的核苷酸序列编码的氨基酸序列或其部分,其中所述核苷酸序列与一种本发明核酸序列的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,将包括采用上述值作为上限和/或下限的任何组合的标识值范围。本发明优选的HA蛋白也最好具有本文所述的至少一种HA活性。例如,本发明的优选HA蛋白包括由与一种本发明核苷酸序列杂交、例如在严格条件下杂交的核苷酸序列编码的氨基酸序列,并且可以参与维持谷氨酸棒杆菌内稳态或可以执行参与该微生物对不同环境条件适应的功能,或者具有表1中所述的一种或多种活性。
在其它实施方案中,所述HA蛋白与一种本发明的氨基酸序列(例如序列表中的偶数SEQ ID NO序列)基本上同源,并且保留本发明氨基酸序列之一的蛋白的功能活性,但由于天然变异或诱变在氨基酸序列有所不同,如以上I小节中详细描述的。因此,在另一实施方案中,所述HA蛋白是这样一种蛋白质,所述蛋白质所具有的氨基序列与一种本发明的完整氨基酸序列的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高,并且具有本文所述的至少一种HA活性。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,将包括采用上述值作为上限和/或下限的任何组合的标识值范围。在另一实施方案中,本发明涉及与一种本发明的完整氨基酸序列基本上同源的全长的谷氨酸棒杆菌蛋白。
HA蛋白的生物活性部分包括包含衍生自HA蛋白的氨基酸序列(例如序列表中偶数SEQ ID NO的氨基酸序列)的氨基酸序列、或与HA蛋白同源的蛋白的氨基酸序列的肽,这包括小于全长HA蛋白或与HA蛋白同源的全长蛋白质、并且表现出至少一种HA蛋白活性的氨基酸。通常,生物活性部分(肽,例如长度例如为5个、10个、15个、20个、30个、35个、36个、37个、38个、39个、40个、50个、100个或更多个氨基酸的肽)包含具有至少一种HA蛋白的活性的结构域或基序。此外,其中所述蛋白质的其它区缺失的其它生物活性部分可以通过重组技术制备,并且根据本文所述的一种或多种活性进行评价。最好是,HA蛋白的生物活性部分包括具有生物活性的一种或多种选定结构域/基序或其部分。
HA蛋白最好通过重组DNA技术产生。例如,将编码所述蛋白质的核酸分子克隆到表达载体(如上所述)中,将所述表达载体导入宿主细胞(如上所述),然后在所述宿主细胞中表达所述HA蛋白。然后可以通过合适的纯化方案,采用标准蛋白质纯化技术,从所述细胞中分离出所述HA蛋白。作为重组表达的替代方法,可以用标准肽合成技术,化学合成HA蛋白、多肽或肽。此外,可以例如采用通过标准技术、利用本发明的HA蛋白或其片段产生的抗HA抗体,从细胞(例如内皮细胞)中分离天然HA蛋白。
本发明也提供HA嵌合蛋白或融合蛋白。本文所用的HA“嵌合蛋白”或“融合蛋白”包含与非HA多肽有效连接的HA多肽。“HA多肽”是指具有对应于HA蛋白的氨基酸序列的多肽,而“非HA多肽”是指所具有的氨基酸序列对应于与所述HA蛋白基本上不同源的蛋白质的多肽,例如不同于所述HA蛋白并且得自同一生物或不同生物的蛋白质。在所述融合蛋白中,术语“有效连接的”甚至是指所述HA多肽和所述非HA多肽相互符合读框地融合。所述非HA多肽可以融合至所述HA多肽的N末端或C末端。例如,在一个实施方案中,所述融合蛋白是其中HA序列融合至GST序列的C末端的GST-HA融合蛋白。这类融合蛋白可以便于纯化重组HA蛋白。在另一实施方案中,所述融合蛋白是在其N末端含有一个异源信号序列的HA蛋白。在某些宿主细胞(例如哺乳动物宿主细胞)中,可以通过利用异源信号序列增加HA蛋白的表达和/或分泌。
最好是,本发明的HA嵌合蛋白或融合蛋白采用标准重组DNA技术来生产。例如,依照常规技术,例如通过利用平端或交错切口末端进行连接,进行限制性酶消化以提供合适的末端,适当地补平粘性末端,进行碱性磷酸酶处理以避免不希望有的连接,然后进行酶连接,将编码不同多肽序列的DNA片段符合读框地连接在一起。在另一实施方案中,通过常规技术,包括自动DNA合成仪,可以合成所述融合基因。或者,采用锚定引物进行基因片段的PCR扩增,所述锚定引物在两个连续基因片段之间产生互补突出端,随后将其退火并重新扩增,产生嵌合基因序列(参见例如Current Protocols in Molecular Biology,Ausubel等编著,John Wiley & Sons:1992)。此外,许多表达载体是市售的,已经编码一个融合部分(例如GST多肽)。可以将HA编码核酸克隆到这样一种表达载体中,使得所述融合部分与所述HA蛋白符合读框地连接。
通过对所述HA蛋白进行诱变,例如离散的点突变或截短,可以产生所述HA蛋白的同系物。本文所用的术语“同系物”是指用作所述HA蛋白活性激动剂或拮抗剂的所述HA蛋白的变异形式。所述HA蛋白的激动剂可以基本上保留所述HA蛋白的相同生物活性或一个亚类的生物活性。所述HA蛋白的拮抗剂通过例如竞争性地与包括所述HA蛋白在内的生物化学级联下游或上游成员结合,或通过结合于HA蛋白与之相互作用的靶分子,使得不可能发生功能性相互作用,或通过直接与HA蛋白结合并且抑制其正常活性,可以抑制天然存在形式的所述HA蛋白的一种或多种活性。
在一个替代实施方案中,通过筛选具有HA蛋白激动剂或拮抗剂活性的所述HA蛋白的突变体例如截短突变体的组合文库,可以鉴定出所述HA蛋白的同系物。在一个实施方案中,通过在核酸水准上进行联合诱变,产生HA变异体的一个花斑文库(variegated library),所述花斑文库由一个花斑基因文库编码。通过例如酶法将合成的寡核苷酸混合物连接到基因序列中,使得一组简并潜在的HA序列可作为单个多肽表达,或者作为其中含有一组HA序列的一组较大的融合蛋白表达(例如用于噬菌体展示),可以产生HA变异体花斑文库。有多种方法可以用来由简并的寡核苷酸序列产生潜在的HA同系物文库。可以在自动DNA合成仪中进行简并基因序列的化学合成,然后将所述合成基因连接到合适的表达载体中。应用一组简并基因,使得可以在一种混合物中提供所有编码一组所需潜在HA序列的序列。合成简并寡核苷酸的方法是本领域已知的(参见例如Narang,S.A.(1983)Tetrahedron39:3;Itakura等(1984)Annu.Rev.Biochem.53:323;Itakura等(1984)Science 198:1056;Ike等(1983)Nucleic Acid Res.11:477)。
另外,所述HA蛋白编码的片段的文库可以用来产生HA片段的花斑群,用来筛选和随后选择HA蛋白的同系物。在一个实施方案中,通过在每个分子仅发生一个缺口的条件下用核酸酶处理HA编码序列的双链PCR片段,使所述双链DNA变性,将所述DNA复性,以形成可以包括来自不同缺口产物的有义/反义对的双链DNA,通过用S1核酸酶处理从重新形成的双链体中去除单链部分,然后将所产生的片段文库连接到表达载体中,可以产生一个编码序列片段文库。用这种方法,可以得到一个表达文库,所述表达文库编码各种大小的所述HA蛋白的N末端片段、C末端片段和内部片段。
本领域已知几种技术用于筛选通过点突变或截短制备的组合文库的基因产物、以及筛选具有选定特性的基因产物的cDNA文库。这类技术适用于快速筛选通过组合诱变HA同系物产生的基因文库。适合于高通量分析、用于筛选大基因文库的最为广泛使用的技术,通常包括将所述基因文库克隆到复制型表达载体中,用所产生的载体文库转化合适的细胞,并且使所述组合基因在所需活性的检测有助于载体分离的条件下表达,而所述载体编码其基因产物被检测的基因。循环总体诱变(REM)是一种提高文库中功能型突变体频率的新技术,可以与鉴定HA同系物的筛选测定结合使用(Arkin和Yourvan(1992)PNAS89:7811-7815;Delgrave等(1993)Protein Engineering 6(3):327-331)。
在另一实施方案中,可以采用本领域中众所周知的方法,用基于细胞的测定来分析花斑HA文库。D.本发明的应用和方法
本文所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可以用于一种或多种以下方法中:谷氨酸棒杆菌和相关生物的鉴定;与谷氨酸棒杆菌相关的生物的基因组作图;所感兴趣的谷氨酸棒杆菌序列的鉴定和定位;进化研究;功能所需的HA蛋白区的测定;HA蛋白活性的调节;一种或多种无机化合物代谢的调节;对一种或多种芳族或脂族化合物修饰或降解的调节;对细胞壁合成或重排的调节;对酶活性或蛋白水解的调节;以及细胞产生所需化合物(例如精细化学品)的调节。
本发明的HA核酸分子具有多种用途。首先,它们可以用来鉴定作为谷氨酸棒杆菌或其密切相关菌种的生物。此外,它们可以用来鉴定微生物混合群体中谷氨酸棒杆菌或其相关菌种的存在。本发明提供许多谷氨酸棒杆菌基因的核酸序列;通过在严格条件下用跨越该生物特有的谷氨酸棒杆菌基因区域的探针,探测特有微生物群体或混合微生物群体培养物的所提取的基因组DNA,人们可以确定该生物是否存在。虽然谷氨酸棒杆菌自身是非致病性的,但它与致病菌种例如白喉棒杆菌相关。白喉棒杆菌是一种快速发展的、涉及局部和系统病理学的急性热性感染一白喉的病原体。在这种疾病中,局部病变在上呼吸道中发展,包括对上皮细胞的坏死性损伤;所述杆菌分泌毒素,毒素通过这种损害弥散到机体的远端易感组织。所产生的变性性改变通过抑制包括心脏、肌肉、外周神经、肾上腺、肾、肝和脾在内的这些组织中的蛋白质合成,导致该疾病的系统病理学。白喉在世界许多地区包括非洲、亚洲、欧洲东部和前苏联许多独立的州中发病率一直很高。自从1990年以来,在后两个地区的一次白喉流行已经导致至少5,000人死亡。
在一个实施方案中,本发明提供一种鉴定受治疗者体内白喉棒杆菌的存在或活性的方法。这种方法包括检测受治疗者体内一种或多种本发明核酸序列或氨基酸序列(例如分别为序列表中奇数或偶数SEQID NO中所示的序列),由此检测所述受治疗者体内白喉棒杆菌的存在或活性。谷氨酸棒杆菌和白喉棒杆菌是相关细菌,谷氨酸棒杆菌中的许多核酸和蛋白质分子与白喉棒杆菌核酸和蛋白质分子同源,因此可以用来检测受治疗者体内的白喉棒杆菌。
本发明的核酸分子和蛋白质分子也可以用作基因组特定区的标记。这不仅可应用于基因组作图中,也可应用于谷氨酸棒杆菌蛋白的功能研究。例如,为了鉴定基因组中特定谷氨酸棒杆菌DNA结合蛋白所结合的区域,可以将谷氨酸棒杆菌基因组消化,然后将所述片段与所述DNA结合蛋白一起温育。结合所述蛋白的那些区域可以另外用本发明的核酸分子来探测,最好是用可容易检测的标记来探测;这种核酸分子与所述基因组片段的结合使得能够将上述片段定位至谷氨酸棒杆菌的基因组图谱上,并且当用不同酶进行多次时,有助于所述蛋白所结合的核酸序列的快速测定。此外,本发明的核酸分子可能与相关菌种的序列具有足够的同源性,因此这些核酸分子可以用作在相关细菌例如乳发酵短杆菌中构建基因组图谱的标记。
本发明的HA核酸分子也可用于进化研究和蛋白质结构研究。涉及本发明分子所参与的适应和稳态维持的过程为种类繁多的物种所利用;通过将本发明核酸分子的序列与来自其他生物的编码相似酶的那些序列进行比较,可以评价所述生物的进化相关性。同样,这种比较允许评价所述序列的哪些区保守、哪些区不保守,这有助于确定酶功能所必需的蛋白区。这种类型的测定对于蛋白质工程研究是有价值的,并且可以给出蛋白质在诱变方面可以耐受而不丧失功能的指标。
对本发明的HA核酸分子的操作可以导致产生功能上不同于野生型HA蛋白的HA蛋白。这些蛋白质可能效率方面或活性方面得到改进,在细胞中的存在数量可能大于通常的数量,或可能效率或活性降低。
本发明提供筛选方法,筛选或者通过与蛋白质自身或所述HA蛋白的底物或者结合配偶体相互作用、或者通过调节本发明HA核酸分子的转录或翻译而调节HA蛋白活性的分子。在这类方法中,使表达一种或多种本发明HA蛋白的微生物与一种或多种试验化合物接触,并且评价每种试验化合物对所述HA蛋白活性或表达水平的影响。
对参与细胞壁生物合成或重排的HA蛋白活性或数量进行调节,可能影响谷氨酸棒杆菌细胞生产一种或多种精细化学品的产量、收率和/或产率。例如,通过改变这些蛋白质的活性,有可能调节细胞壁的结构或厚度。细胞壁主要用作抗渗透裂解和外源损伤的保护性装置;通过修饰细胞壁,有可能提高谷氨酸棒杆菌抵抗在大规模发酵罐培养期间该微生物遇到的机械应力和剪切应力的能力。此外,每个谷氨酸棒杆菌细胞被厚厚的细胞壁围绕,因此,大规模培养物中存在的生物质的相当大的一部分由细胞壁组成。通过提高细胞壁的合成速率,或通过活化细胞壁的合成(通过对本发明HA细胞壁蛋白进行基因工程),有可能提高所述微生物的生长速率。同样,通过降低参与细胞壁降解的蛋白质的活性或数量,或通过降低对细胞壁生物合成的阻抑,可以达到细胞壁产生的整体增加。活谷氨酸棒杆菌细胞数量的增加(这可以通过任何上述蛋白质的改变来完成),应该导致大规模发酵罐培养物中生产所需精细化学品的细胞数量增加,这应该使得得自所述培养物的这些化合物生产的收率或产率提高。
对参与修饰或降解芳族或脂族化合物的谷氨酸棒杆菌HA蛋白活性或数量进行调节,也可以对这些细胞的一种或多种精细化学品的产生具有直接或间接的影响。某些芳族或脂族修饰或降解产物是理想的精细化学品(例如有机酸或经修饰的芳族和脂族化合物);因此,通过修饰进行这些修饰(例如羟化、甲基化或异构化)或降解反应的酶,有可能提高这些所需化合物的收率。同样,通过降低参与进一步降解上述反应的经修饰或分解产物的途径的蛋白质的活性或数量,有可能提高得自培养物中谷氨酸棒杆菌细胞的这些精细化学品的收率。
这些芳族和脂族修饰酶和降解酶本身是精细化学品。纯化形式的这些酶可以用来降解芳族和脂族化合物(例如有毒化学物质,例如石油产品),或者用于污染现场的生物除污,用于废物的工程分解,或用于大规模和经济上可行的所需经修饰的芳族或脂族化合物或其分解产物的生产,某些上述物质可以方便地用作培养物中其它精细化学品生产化合物的碳源或能源(参见例如Feber,K.(1995)Biotransformations inOrganic Chemistry,Springer:Berlin和其中的参考文献;和Roberts,S.M.编著(1992-1996)Preparative Biotransformations,Wiley:Chichester和其中的参考文献)。通过对这些蛋白质进行遗传改变,使得其它细胞机制对它们的调节得以减轻或被消除,有可能增加这些蛋白质的总体数量或活性,由此不仅提高这些精细化学品的收率,而且提高这些收获的蛋白质的活性。
对这些芳族和脂族修饰酶和降解酶进行修饰,也可以对一种或多种精细化学品的产生具有间接影响。许多芳族和脂族化合物(例如可能作为培养基中的杂质或作为来自细胞代谢的废物而遇到的那些化合物)对细胞有毒性;通过修饰和/或降解这些化合物,使得它们可以被容易地除去或破坏,应该提高细胞的生存力。此外,这些酶可以修饰或降解这些化合物,其修饰或降解方式使得所得产物可以进入细胞正常的碳代谢途径,因此使得细胞能够利用这些化合物作为替代碳源或能源。在大规模培养情况下,当存在限制量的最适碳源时,这些酶提供了细胞可以利用芳族或脂族化合物作为养分继续生长和分裂的方法。在这两种情况下,所导致的培养物中生产所需精细化学品的谷氨酸棒杆菌细胞数量增加,应该进而导致所述精细化学品生产的收率或产率的提高。
对参与无机化合物代谢的HA蛋白的活性或数量进行修饰,也可以直接或间接地影响谷氨酸棒杆菌或相关细菌培养物的一种或多种精细化学品的生产。例如,许多理想的精细化学品例如核酸、氨基酸、辅因子和维生素(例如硫胺素、生物素和硫辛酸)在无无机分子例如磷、硝酸盐、硫酸盐和铁的情况下不能合成。本发明的无机代谢蛋白使得细胞可以从各种各样的无机化合物获得这些分子,并且将它们转移到各种精细化学品生物合成途径中。因此,通过提高参与这些无机化合物代谢的酶的活性或数量,有可能增加这些可能的限制性无机分子的供应,由此直接提高含这类经改变蛋白质的谷氨酸棒杆菌细胞生产各种精细化学品的产量或产率。对本发明无机代谢酶的活性或数量进行修饰,也可以使得谷氨酸棒杆菌能够更好地利用有限的无机化合物供应,或利用非最适无机化合物来合成氨基酸、维生素、辅因子或核酸,所有这些都是细胞继续生长和复制必不可少的。通过提高大规模培养物中这些细胞的生存力,也可以增加培养物中生产一种或多种精细化学品的谷氨酸棒杆菌细胞的数量,进而提高一种或多种精细化学品生产的收率或产率。
通用过程的谷氨酸棒杆菌酶本身是理想的精细化学品。酶的特定特性(即其中包括区域专一性和立体专一性)使其成为体外化学反应的有用催化剂。或者可以将谷氨酸棒杆菌全细胞与合适的底物一起温育,使得在所述细胞中通过酶生产所需产物,或者可以使谷氨酸棒杆菌培养物(或相关细菌的培养物)过量产生所需酶并且将其纯化,随后用于工业环境的体外反应中(或者用于溶液中或者固定化在合适的固定相上)。在两种情况下,所述酶可以或者是天然的谷氨酸棒杆菌蛋白质,或者可以将其诱变,以具有改变的活性;这类酶的典型的工业应用包括在化学工业中作为催化剂(例如用于合成有机化学)、作为食品添加剂、饲料组分、用于水果加工、制革、去垢剂、用于分析和药物以及用于纺织工业(参见例如Yamada,H.(1993)“用于生产有用的有机化合物的微生物反应”,Chimica 47:5-10;Roberts,S.M.(1998)“制备性生物转化:酶和全细胞在合成化学中的应用”,J.Chem.Soc.PerkinTrans.1:157-169;Zaks,A.和Dodds,D.R.(1997)“应用生物催化和生物转化合成药物”,DDT 2:513-53l;Roberts,S.M.和Williamson,N.M.(1997)“酶在生物活性天然产物和旋光活性形式的类似物制备中的应用”,Curr.Organ.Chemistry 1:1-20;Faber,K.(1995)Biotransformationsin Organic Chemistry,Springer:Berlin;Roberts,S.M.编著(1992-96)Preparative Biotransformations,Wiley:Chichester;Cheetham,P.S.J.(1995)“工业中的酶应用”,载于:Handbook of Enzyme Biotechnology,第3版,Wiseman,A.编著,Elis:Horwood,第419-552页;和Ullmann’sEncyclopedia of Industrial Chemistry(1987)第A9卷,Enzymes,第390-457页)。因此,通过提高这些酶的活性或数量,有可能也提高了细胞将所供应的底物转化为所需产物的能力,或有可能过量产生这些酶以提高大规模培养物中的收率。此外,通过对这些蛋白质进行诱变,有可能去除反馈抑制或其它阻抑性细胞调节控制,使得细胞可以产生并且活化更大量的这些酶,由此导致得自大规模培养的这些精细化学品蛋白质生产的收率、产量或产率更高。而且,对这些酶的操纵可以改变一种或多种谷氨酸棒杆菌代谢途径、例如一种或多种精细化学品生物合成或分泌的途径的活性。
对本发明蛋白水解酶进行诱变使得它们在活性或数量上改变,也可以直接或间接地影响谷氨酸棒杆菌生产一种或多种精细化学品的收率、产量和/或产率。例如,通过增加这些蛋白质的活性或数量,有可能由于该细胞快速降解对高温度、非最适pH和发酵罐培养物期间遭遇的其它胁迫而应答错折叠的蛋白质的能力增加,而提高该细菌在大规模培养中的生存能力。这些培养物中细胞数量的增加由于所述培养物中生产一种或多种精细化学品的细胞数量相对较大,因此可以导致这些化合物生产的收率或产率提高。另外,谷氨酸棒杆菌细胞具有多种细胞表面蛋白酶,它们用来将外来养分分解为细胞容易将其作为碳源/能源或其它种类的养分掺入的分子。这些酶的活性或数量的增加,可以改进这种周转(turnover),并且提高有效养分的水平,由此改进细胞生长或生产。因此,对本发明蛋白酶的修饰,可以间接地影响谷氨酸棒杆菌的精细化学品生产。
当本发明蛋白酶参与所需精细化学品的产生或降解时,可以对响应于一种或多种本发明蛋白酶修饰的精细化学品生产产生更为直接的影响。通过降低降解精细化学品或降解参与精细化学品合成的蛋白质的蛋白酶的活性,有可能提高该精细化学品的水平(由于所述化合物的降解降低或合成增加)。同样,通过将降解产生精细化学品或产生参与精细化学品降解的蛋白质的化合物的蛋白酶的活性提高,应该达到相似的结果:得自含有这些工程改造的蛋白质的谷氨酸棒杆菌细胞的所需精细化学品水平增加。
导致得自谷氨酸棒杆菌的精细化学品收率提高的上述HA蛋白诱变策略,并不意味着是限制性的;这些策略的变化对于本领域技术人员是显而易见的。采用这些策略并且加入本文公开的机制,可以利用本发明的核酸分子和蛋白质分子来产生表达突变型HA核酸分子和蛋白质分子的谷氨酸棒杆菌或相关细菌菌株,使得所需化合物生产的收率、产量和/或产率得以改善。这种所需化合物可以是谷氨酸棒杆菌产生的任何产物,包括生物合成途径的终产物和天然存在的代谢途径的中间体、以及在谷氨酸棒杆菌代谢中不是天然存在的、但由本发明的谷氨酸棒杆菌菌株产生的分子。
本发明通过以下实施例进一步说明,所述实施例不应解释为是限制性的。在本申请中引用的所有参考文献、专利申请、专利、公布的专利申请、表和序列表的内容,通过引用结合到本文中。
表1:本申请中的基因核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO1 2 RXA02548 GR00727 3 293 硫酸腺苷酸转移酶亚基2(EC 2.7.7.4)3 4 RXN00249 VV0057 36825 35869 腺苷酰硫酸激酶(EC 2.7.1.25)5 6 FRXA00249 GR00037 8837 7884 腺苷酰硫酸激酶(EC 2.7.1.25)7 8 RXA01073 GR00300 1274 2104 NH(3)依赖性NAD(+)合成酶(EC 6.3.5.1)脲酶核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO9 10 RXN02913 VV0020 8998 8513 脲酶β亚基(EC 3.5.1.5)11 12 FRXA02264 GR00655 123 4 脲酶α亚基(EC 3.5.1.5)13 14 RXN02274 VV0020 8509 6800 脲酶α亚基(EC 3.5.1.5)15 16 FRXA02274 GR00656 3 1604 脲酶α亚基(EC 3.5.1.5)17 18 RXA02265 GR00655 452 153 脲酶γ亚基(EC 3.5.1.5)19 20 RXA02278 GR00656 3420 4268 脲酶操纵子URED蛋白21 22 RXA02275 GR00656 1632 2102 脲酶辅助蛋白UREE23 24 RXA02276 GR00656 2105 2782 脲酶辅助蛋白UREF25 26 RXA02277 GR00656 2802 3416 脲酶辅蛋白UREG27 28 RXA02603 GR00742 7742 8737 4-羟基苯甲酸八异戊二烯基转移酶(EC 2.5.1.-)29 30 RXA01385 GR00406 5320 3440 酚2单加氧酶(EC 1.14.13.7)蛋白水解核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO31 32 RXN00675 VV0005 33258 34049 甲硫氨酸氨肽酶(EC 3.4.11.18)33 34 FRXA00675 GR00178 2 484 甲硫氨酸氨肽酶(EC 3.4.11.18)35 36 RXA01609 GR00449 2740 3612 甲硫氨酸氨肽酶(EC 3.4.11.18)37 38 RXA01358 GR00393 5337 6857 ATP依赖性蛋白酶LA(EC 3.4.21.53)39 40 RXA01458 GR00420 3225 2176 ATP依赖性蛋白酶LA(EC 3.4.21.53)41 42 RXA01654 GR00459 986 1981 (AL022121)推定的碱性丝氨酸蛋白酶[结核分枝杆菌]43 44 RXN01868 VV0127 9980 11905 锌金属蛋白酶(EC 3.4.24.-)45 46 FRXA01868 GR00534 1640 30 锌金属蛋白酶(EC 3.4.24.-)47 48 FRXA01869 GR00534 1954 1652 锌金属蛋白酶(EC 3.4.24-)49 50 RXN03028 VV0008 41156 43930 ATP依赖性CLP蛋白酶ATP结合亚基CLPA51 52 FRXA02470 GR00715 2216 3196 ATP依赖性CLP蛋白酶ATP结合亚基CLPA
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO53 54 FRXA02471 GR00715 3159 4991 ATP依赖性CLP蛋白酶ATP结合亚基CLPA55 56 RXA02630 GR00748 2654 1332 (AL021999)推定的丝氨酸蛋白酶[结核分枝杆菌]57 58 RXA02834 GR00823 3 497 具有侣伴蛋白活性的ATP酶,ATP依赖性蛋白酶亚基59 60 RXA00112 GR00016 3687 2497 可能的周质丝氨酸蛋白酶DO样前体61 62 RXA00566 GR00152 742 137 ATP依赖性CLP蛋白酶蛋白水解亚基(EC 3.4.21.92)63 64 RXA00567 GR00152 1388 798 ATP依赖性CLP蛋白酶蛋白水解亚基(EC 3.4.21.92)65 66 RXN03094 VV0057 1794 43 CLPB蛋白67 68 FRXA01668 GR00464 2205 3920 CLPB蛋白69 70 RXN01120 VV0182 5678 4401 ATP依赖性CLP蛋白酶ATP结合亚基CLPX71 72 FRXA01120 GR00310 2349 1072 ATP依赖性CLP蛋白酶ATP结合亚基CLPX73 74 RXA00744 GR00202 10722 9781 周质丝氨酸蛋白酶75 76 RXA00844 GR00228 3620 4453 假定的分泌型丝氨酸蛋白酶(EC 3.4.21.-)77 78 RXA01151 GR00324 862 5 ATP依赖性Zn蛋白酶79 80 RXA02317 GR00665 9664 9053 肽酶E(EC 3.4.-.-)81 82 RXA02644 GR00751 767 117 Xaa-Pro二肽酶(EC 3.4.13.9)83 84 RXN02820 VV0131 4799 6109 γ-谷氨酰转肽酶(EC 2.3.2.2)85 86 FRXA02820 GR00801 1 507 γ-谷氨酰转肽酶(EC 2.3.2.2)87 88 FRXA02000 GR00589 3430 3933 γ-谷氨酰转肽酶(EC 2.3.2.2)89 90 RXN03178 VV0334 921 121 青霉素结合蛋白5*前体(D-丙氨酰-D-丙氨酸羧肽酶)(EC 3.4.16.4)91 92 FRXA02859 GR10005 846 121 青霉素结合蛋白5*前体(D-丙氨酰-D-丙氨酸羧肽酶)(EC 3.4.16.4)93 94 RXA00137 GR00022 738 1826 Xaa-Pro氨基肽酶(EC 3.4.11.9)95 96 RXN00499 VV0086 8158 9438 脯氨酸亚氨基肽酶(EC 3.4.11.5)97 98 FRXA00499 GR00125 3 959 脯氨酸亚氨基肽酶99 100 RXN00877 VV0099 2221 3885 肽基二肽酶DCP(EC 3.4.15.5)101 102 FRXA00877 GR00242 3 1067 肽基二肽酶DCP(EC 3.4.15.5)103 104 RXN01014 VV0209 13328 10728 氨基肽酶N(EC 3.4.11.2)105 106 FRXA01014 GR00289 3 1580 氨基肽酶N(EC 3.4.11.2)107 108 FRXA01018 GR00290 2289 3152 氨基肽酶N(EC 3.4.11.2)109 110 RXA01147 GR00323 1353 94 液泡氨基肽酶I前体(EC 3.4.11.1)111 112 RXA01161 GR00329 1253 117 Xaa-Pro氨基肽酶(EC 3.4.11.9)113 114 RXN01181 VV0065 1 957 氨基肽酶A/I(Ec 3.4.11.1)115 116 FRXA01181 GR00337 1 957 氨基肽酶117 118 RXN01277 VV0009 32155 34158 脯氨酰内肽酶(EC 3.4.21.26)119 120 FRXA01277 GR00368 1738 50 脯氨酰内肽酶(EC 3.4.21.26)121 122 RXA01914 GR00548 125 550 氨基肽酶123 124 RXA02048 GR00624 207 1580 氨基肽酶N(EC 3.4.11.2)125 126 RXN00621 VV0135 5853 5071 蛋白酶II(EC 3.4.21.83)127 128 FRXA00621 GR00163 4075 4857 PTRB周质蛋白酶129 130 RXN00622 VV0135 5150 3735 蛋白酶II(EC 3.4.21.83)131 132 FRXA00622 GR00163 4778 6193 PTRB周质蛋白酶133 134 RXN00982 VV0149 7596 6091 (L42758)蛋白酶[浅青紫链霉菌]135 136 FRXA00977 GR00275 1647 2660 (L42758)蛋白酶[浅青紫链霉菌]137 138 FRXA00982 GR00276 5194 4949 (L42758)蛋白酶[浅青紫链霉菌]139 140 RXA00152 GR00023 7175 5880 HFLC蛋白(EC 3.4.-.-)141 142 RXA02558 GR00731 4939 3965 HFLC蛋白(EC 3.4.-.-)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO143 144 RXA00500 GR00125 969 1643 O-唾液酸糖蛋白内肽酶(EC 3.4.24.57)145 146 RXA00501 GR00125 1643 2149 O-唾液酸糖蛋白内肽酶(EC 3.4.24.57)147 148 RXA00502 GR00125 2156 3187 O-唾液酸糖蛋白内肽酶(EC 3.4.24.57)通用的酶核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO149 150 RXN02589 VV0098 16346 17110 假定的甲基转移酶(EC 2.1.1.-)151 152 FRXA02589 GR00741 13804 13040 预测的S-腺苷甲硫氨酸依赖性甲基转移酶153 154 RXA00226 GR00032 26836 26012 SAM依赖性甲基转移酶155 156 RXN01885 VV0184 2004 2804 假定的甲基转移酶(EC 2.1.1.-)157 158 FRXA01885 GR00539 1589 2389 SAM依赖性甲基转移酶159 160 RXA02592 GR00741 18477 17707 SAM依赖性甲基转移酶161 162 RXN01795 VV0093 722 1318 修饰性甲基化酶(EC 2.1.1.73)163 164 FRXA01795 GR00507 706 1140 修饰性甲基化酶(EC 2.1.1.73)165 166 RXA01214 GR00351 1640 3130 漆酶1前体(EC 1.10.3.2)167 168 RXA01250 GR00364 592 5 漆酶1前体(EC 1.10.3.2)169 170 RXA02477 GR00715 10581 11201 碳酸酐酶(EC 4.2.1.1)171 172 RXN00833 GR00225 374 6 硫醇过氧化物酶(EC 1.11.1.-)173 174 FRXA00833 GR00225 374 6 硫醇过氧化物酶(EC 1.11.1.-)175 176 RXA01224 GR00354 4186 5208 2-硝基丙烷双加氧酶(EC 1.13.11.32)177 178 RXA01182 GR00337 1363 971 假定的氧化还原酶179 180 RXA02531 GR00726 1226 1936 假定的氧化还原酶181 182 RXN00689 VV0005 22416 20926 甜菜碱-醛脱氢酶前体(EC 1.2.1.8)183 184 FRXA00689 GR00180 1401 775 甜菜碱-醛脱氢酶前体(EC 1.2.1.8)185 186 RXN03128 VV0120 3 857 吗啡6-脱氢酶(EC 1.1.1.218)187 188 FRXA02192 GR00643 2 523 吗啡6-脱氢酶(EC 1.1.1.218)189 190 RXA02351 GR00679 132 1070 次氮基三乙酸单加氧酶组分A(EC 1.14.13.-)191 192 RXN00905 VV0238 8075 8875 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)193 194 FRXA00905 GR00247 2 694 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)195 196 RXA00906 GR00247 630 1133 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)197 198 RXA00907 GR00247 1143 1265 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)199 200 RXA02101 GR00631 3104 1842 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)201 202 RXN02565 VV0154 14299 13034 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)203 204 FRXA02565 GR00733 1 342 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)205 206 FRXA02567 GR00734 3 740 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)207 208 RXN03077 VV0043 1729 2913 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)209 210 FRXA02855 GR10002 1693 2877 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14),马尿酸水解酶211 212 RXA00026 GR00003 3657 5042 假定的酰胺水解酶(EC 3.5.1.-)213 214 RXA01971 GR00569 963 133 假定的金属依赖性水解酶215 216 RXA01802 GR00509 3461 4291 预测的水解酶(HAD超家族)217 218 RXN00866 VV0258 3557 4522 预测的Zn依赖性水解酶219 220 FRXA00866 GR00236 3555 4499 预测的Zn依赖性水解酶221 222 RXA02410 GR00703 792 127 预测的Zn依赖性水解酶
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO223 224 RXA00961 GR00267 2 433 水杨酸羟化酶(EC 1.14.13.1)225 226 RXA00111 GR00016 930 1922 可溶性环氧化物酶(SHE)(EC 3.3.2.3)227 228 RXA01932 GR00555 6479 5583 乙酰水解酶(EC 3.1.1.-)229 230 RXA02574 GR00739 833 1840 推定的分泌型水解酶231 232 RXN00983 VV0231 1796 321 唾液酸酶前体(EC 3.2.1.18)233 234 FRXA00983 GR00278 1200 4 唾液酸酶前体(EC 3.2.1.18)235 236 RXA00984 GR00278 1716 1300 唾液酸酶前体(EC 3.2.1.18)237 238 RXN02513 VV0193 737 6 唾液酸酶前体(EC 3.2.1.18)239 240 FRXA02513 GR00722 93 824 唾液酸酶前体(EC 3.2.1.18)241 242 RXA00903 GR00246 637 5 推定的差向异构酶243 244 RXA01224 GR00354 4186 5208 2-硝基丙烷双加氧酶(EC 1.13.11.32)245 246 RXA01571 GR00438 1360 1959 醇脱氢酶(EC 1.1.1.1)247 248 RXN02478 VV0119 7564 6350 唾液酸酶前体(EC 3.2.1.18)249 250 RXN00343 VV0125 1118 6 3-氧类固醇1-脱氢酶(EC 1.3.99.4)251 252 RXN01555 VV0135 29820 28861 3-氧类固醇1-脱氢酶(EC 1.3.99.4)253 254 RXN01166 VV0117 18142 16838 胞外脂酶前体(EC 3.1.1.3)255 256 RXN02001 VV0326 630 1787 N-酰基-L-氨基酸酰胺水解酶(EC 3.5.1.14)257 258 RXN03145 VV0142 7561 7115 4-草酰巴豆酸互变异构酶(EC 5.3.2.-)259 260 RXN01466 VV0019 7050 6091 芳基酯酶(EC 3.1.1.2)261 262 RXN01145 VV0077 7538 6525 酮酸还原异构酶(EC 1.1.1.86)263 264 RXN03088 VV0052 3431 3817 假定的甲基转移酶(EC 2.1.1.-)265 266 RXN02952 VV0320 1032 1547 推定的还原酶267 268 RXN00513 VV0092 1573 653 羧基乙烯基-羟基膦酸磷酸变位酶(EC 2.7.8.23)269 270 RXN01152 VV0136 1740 907 蛋白质-L-异天冬氨酸O-甲基转移酶(EC 2.1.1.77)271 272 RXN00787 VV0321 3736 5637 D-氨基酸脱氢酶大亚基(EC 1.4.99.1)N-代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO273 274 RXN01302 VV0148 2837 2385 硝酸还原酶α链(EC 1.7.99.4)275 276 FRXA01302 GR00376 370 5 硝酸还原酶α链(EC 1.7.99.4)277 278 RXN01308 VV0148 2406 4 硝酸还原酶α链(EC 1.7.99.4)279 280 FRXA01307 GR00377 686 6 硝酸还原酶α链(EC 1.7.99.4)281 282 FRXA01308 GR00378 1211 6 硝酸还原酶α链(EC 1.7.99.4)283 284 RXN01309 VV0158 1 801 硝酸还原酶α链(EC 1.7.99.4)285 286 FRXA01309 GR00379 719 51 硝酸还原酶α链(EC 1.7.99.4)287 288 RXA02017 GR00610 1731 1048 硝酸还原酶α链(EC 1.7.99.4)289 290 RXA02018 GR00610 2788 1739 硝酸还原酶β链(EC 1.7.99.4)291 292 RXA02016 GR00610 1036 260 硝酸还原酶γ链(EC 1.7.99.4)293 294 RXA00471 GR00119 2997 3686 硝酸/亚硝酸效应调节蛋白NARL295 296 RXA00133 GR00021 201 1013 硝酸/亚硝酸效应调节蛋白NARP297 298 RXA00650 GR00169 4017 3382 硝酸/亚硝酸效应调节蛋白NARP
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO299 300 RXA01189 GR00339 2545 1937 硝酸/亚硝酸效应调节蛋白NARP301 302 RXA01607 GR00449 123 752 硝酸/亚硝酸效应调节蛋白NARP303 304 RXN00470 VV0086 27401 28669 硝酸/亚硝酸感应蛋白NARX(EC 2.7.3.-)305 306 FRXA00470 GR00119 1752 2951 硝酸/亚硝酸感应蛋白NARX(EC 2.7.3.-)307 308 RXA00756 GR00203 2932 1937 N利用物质蛋白A309 310 RXA00139 GR00022 2514 3224 N利用物质蛋白B311 312 RXA01303 GR00376 1724 390 硝酸挤出蛋白313 314 RXA01412 GR00412 620 417 固氮蛋白FIXI(可能的E1-E2型阳离子ATP酶)(EC 3.6.1.-)315 316 RXA00773 GR00205 3208 4350 氮调节蛋白NIFR3317 318 RXA02746 GR00764 1 267 氮调节蛋白P-II319 320 RXA02745 GR00763 15350 14472 结瘤ATP结合蛋白I321 322 RXN00820 VV0054 19455 19817 结瘤蛋白N323 324 FRXA00820 GR00221 1007 1369 结瘤蛋白N325 326 RXA01059 GR00296 8782 9390 氧不敏感性NAD(P)H硝酸还原酶(EC 1.-.-.-)327 328 RXN01386 VV0008 39246 38317 腈水解酶调节蛋白329 330 RXN00073 VV0154 2369 687 铁氧还蛋白-亚硝酸还原酶(EC 1.7.7.1)331 332 RXN03131 VV0127 276 4 根霉促进素分解代谢蛋白MOCC333 334 RXS00153 VV0167 4195 4620 结瘤蛋白脲酶核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO磷酸和膦酸代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO335 336 RXN01716 VV0319 3259 2774 聚磷酸外切酶(EC 3.6.1.11)337 338 RXN02972 VV0319 2763 2353 聚磷酸外切酶(EC 3.6.1.11)339 340 RXN00663 VV0142 10120 11493 PHOH蛋白同系物341 342 RXN00778 VV0103 18126 19250 磷酸结合周质蛋白了前体343 344 RXN00250 VV0189 286 1032 DEDA蛋白-碱性磷酸酶样蛋白硫代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO345 346 RXA00072 GR00012 446 6 磷酸腺苷-磷酰硫酸还原酶(EC 1.8.99.4)347 348 RXA00793 GR00211 1469 2644 硫酸饥饿诱导蛋白6349 350 RXA01192 GR00342 161 733 硫酸饥饿诱导蛋白6
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO351 352 RXA00715 GR00188 2120 2914 硫代硫酸转硫酶(EC 2.8.1.1)353 354 RXA01664 GR00463 1306 485 硫代硫酸转硫酶(EC 2.8.1.1)355 356 RXN02334 VV0141 7939 7217 硫代硫酸转硫酶(EC 2.8.1.1)357 358 FRXA02334 GR00672 2 355 硫代硫酸转硫酶(EC 2.8.1.1)Fe-代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO359 360 RXN01499 VV0008 7034 3213 肠杆菌素合成酶组分F361 362 RXN01997 VV0084 33308 33793 铁蛋白Mg代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO363 364 RXA01848 GR00524 1532 789 镁-螯合酶亚基CHLI365 366 RXN01849 VV0139 16415 17515 镁-螯合酶亚基CHLI367 368 FRXA01849 GR00524 2004 1555 镁-螯合酶亚基CHLI369 370 FRXA01691 GR00474 570 4 镁-螯合酶亚基CHLI371 372 RXN00665 VV0252 135 635 Mg2+/柠檬酸复合体次级转运蛋白(secondary transporter)芳族化合物的修饰和降解核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO373 374 RXN03026 VV0007 28635 28901 3-脱氢奎尼酸脱水酶(EC 4.2.1.10)375 376 RXN02908 VV0025 8507 8247 O-琥珀酰苯甲酸-CoA连接酶(EC 6.2.1.26)377 378 RXN03000 VV0235 570 4 水杨酸羟化酶(EC 1.14.13.1)379 380 RXN03036 VV0014 671 6 原儿茶酸3,4-双加氧酶β链(EC 1.13.11.3)381 382 RXN02974 VV0229 12631 12437 4-硝基苯基磷酸酶(EC 3.1.3.41)383 384 RXN00393 VV0025 7241 6348 1,4-二羟基-2-萘甲酸八异戊二烯基转移酶(EC 2.5.-.-)385 386 RXN00948 VV0107 4266 5384 12-氧植二烯酸(oxophytodienoate)还原酶(EC 1.3.1.42)387 388 RXN01923 VV0020 3384 4133 2-羟基-6-氧-6-苯基己-2,4-二烯酸水解酶(EC 3.7.1.-)389 390 RXN00398 VV0025 14633 13884 2-吡喃酮-4,6-二羧酸内酯酶(EC 3.1.1.57)391 392 RXN02813 VV0128 13120 14118 3-羧基-顺式,顺式-粘康酸环异构酶同系物(EC 5.5.1.2)393 394 RXN00136 VV0134 13373 14467 3-脱氢奎尼酸合酶(EC 4.6.1.3)395 396 RXN02508 VV0007 26733 28586 3-脱氢莽草酸脱水酶(EC 4.2.1.-)397 398 RXN02839 VV0362 3 449 4-羟基苯甲酸八异戊二烯基转移酶(EC 2.5.1.-)399 400 RXN00639 VV0128 7858 8712 儿茶酚1,2-双加氧酶(EC 1.13.11.1)401 402 RXN02530 VV0057 5469 6125 二甲基苯胺单加氧酶(N-氧化物形成)1(EC 1.14.13.8)403 404 RXN00434 VV0112 12078 11212 醌氧化还原酶(EC 1.6.5.5)405 406 RXN01619 VV0050 24649 23675 醌氧化还原酶(EC 1.6.5.5)407 408 RXN01842 VV0234 1615 2532 醌氧化还原酶(EC 1.6.5.5)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO409 410 RXN00641 VV0128 7440 5950 甲苯甲酸1,2-双加氧酶α亚基(EC 1.14.12.-)411 412 RXN01993 VV0182 16 1143 香草酸脱甲基酶(EC 1.14.-.-)413 414 RXN00658 VV0083 15705 16397 酚2-单加氧酶(EC 1.14.13.7)415 416 RXN00178 VV0174 14670 15554 偏苯三酚1,2-双加氧酶(EC 1.13.11.37)417 418 RXN01461 VV0128 12414 13025 原儿茶酸3,4-双加氧酶α链(EC 1.13.11.3)419 420 RXN01653 VV0321 12867 11407 二苯并噻吩脱硫酶A421 422 RXN02053 VV0009 39448 40026 DRGA蛋白423 424 RXN00177 VV0174 13589 14656 马来酰乙酸还原酶(EC 1.3.1.32)425 426 RXC00963 VV0249 1816 2652 参与芳族化合物降解的蛋白质脂族化合物的修饰和降解核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO427 428 RXN00299 VV0176 43379 42402 链烷醛单加氧酶α链(EC 1.14.14.3)429 430 FRXA00299 GR00048 7376 6633 链烷醛单加氧酶α链(EC 1.14.14.3)431 432 RXN00332 GR00057 16086 15385 链烷醛单加氧酶α链(EC 1.14.14.3)433 434 RXA01838 GR00519 2 820 链烷醛单加氧酶α链(EC 1.14.14.3)435 436 RXA02643 GR00750 1603 560 链烷醛单加氧酶α链(EC 1.14.14.3)437 438 RXA01933 GR00555 6590 7192 2-卤代链烷酸脱卤素酶I(EC 3.8.1.2)439 440 RXA02351 GR00679 132 1070 次氮基三乙酸单加氧酶组分A(EC 1.14.13.-)
表2-排除的基因 | |||
GenBankTM登记号 | 基因名称 | 基因功能 | 参考文献 |
A09073 | ppg | 磷酸烯醇丙酮酸羧化酶 | Bachmann,B.等“编码磷酸烯醇丙酮酸羧化酶的DNA片段、携带所述片段的重组DNA、带有所述重组DNA的菌株和采用所述菌株生产L-氨基酸的方法”,专利:EP 0358940-A 3 03/21/90 |
A45579,A45581,A45583,A45585A45587 | 苏氨酸脱水酶 | Moeckel,B.等“利用具有去调节苏氨酸脱水酶的重组微生物生产L-异亮氨酸”专利:WO 9519442-A 5 07/20/95 | |
AB003132 | murC;ftsQ;fisZ | Kobayashi,M.等“来自棒状细菌的ftsZ基因的克隆、测序和特征鉴定”Biochem.Biophys.Res.Commun.,236(2):383-388(1997) | |
AB015023 | murC;ftsQ | Wachi,M.等“来自棒杆菌的murC基因”Appl.Microbiol.Biotechnol.,51(2):223-228(1999) | |
AB018530 | dtsR | Kimura,E.等“拯救衍生自乳发酵短杆菌的突变体去垢剂敏感性的新型基因dtsR的分子克隆”,Biosci.Biotechnol.Biochem.,60(10):1565-1570(1996) | |
AB018531 | dtsR1;dtsR2 | ||
AB020624 | murI | D-谷氨酸消旋酶 | |
AB023377 | tkt | 转羟乙醛酶 | |
AB024708 | gltB;gltD | 谷氨酰胺2-酮戊二酸氨基转移酶大亚基和小亚基 | |
AB025424 | acn | 顺乌头酸酶 | |
AB027714 | rep | 复制蛋白 | |
AB027715 | rep;aad | 复制蛋白;氨基糖苷腺苷酸转移酶 | |
AF005242 | argC | N-乙酰谷氨酸-5-半醛脱氢酶 | |
AF005635 | glnA | 谷氨酰胺合成酶 | |
AF030405 | hisF | 环化酶 | |
AF030520 | argG | 精氨琥珀酸合成酶 | |
AF031518 | argF | 鸟氨酸氨甲酰基转移酶 | |
AF036932 | aroD | 3-脱氢奎尼酸脱水酶 | |
AF038548 | pyc | 丙酮酸羧化酶 | |
表2(续) | |||
AF038651 | dciAE;apt;rel | 二肽结合蛋白;腺嘌呤磷酸核糖转移酶;GTP焦磷酸激酶 | Wehmeier,L.等“谷氨酸棒杆菌rel基因在(p)ppGpp代谢中的作用”,Microbiology,144:1853-1862(1998) |
AF041436 | argR | 精氨酸阻抑蛋白 | |
AF045998 | impA | 肌醇单磷酸磷酸酶 | |
AF048764 | argH | 精氨琥珀酸裂合酶 | |
AF049897 | argC;argJ;argB;argD;argF;argR;argG;argH | N-乙酰谷氨酰磷酸还原酶;鸟氨酸乙酰基转移酶;N-乙酰谷氨酸激酶;乙酰鸟氨酸转氨酶;鸟氨酸氨甲酰转移酶;精氨酸阻抑蛋白;精氨琥珀酸合酶;精氨琥珀酸裂合酶 | |
AF050109 | inhA | 烯酰-酰基载体蛋白还原酶 | |
AF050166 | hisG | ATP磷酸核糖转移酶 | |
AF051846 | hisA | 甲酰亚氨基-5-氨基-1-磷酸核糖基-4-咪唑甲酰胺核苷酸异构酶 | |
AF052652 | metA | 高丝氨酸O-乙酰基转移酶 | Park,S.等“metA-一种谷氨酸棒杆菌中编码高丝氨酸乙酰基转移酶的甲硫氨酸生物合成基因的分离与分析”,Mol.Cells.,8(3):286-294(1998) |
AF053071 | aroB | 脱氢奎尼酸合成酶 | |
AF060558 | hisH | 谷氨酰胺转酰胺酶 | |
AF086704 | hisE | 磷酸核糖基-ATP-焦磷酸水解酶 | |
AF114233 | aroA | 5-烯醇丙酮酸莽草酸3-磷酸合酶 | |
AF116184 | panD | L-天冬氨酸-α-脱羧酶前体 | Dusch,N.等“编码L-天冬氨酸-α-脱羧酶的谷氨酸棒杆菌panD基因的表达导致在大肠杆菌中过量产生泛酸”,Appl.Environ.Microbiol.,65(4)1530-1539(1999) |
AF124518 | aroD;aroE | 3-脱氢奎尼酸酶(dehydroquinase);莽草酸脱氢酶 | |
AF124600 | aroC;aroK;aroB;pepQ | 分支酸合酶;莽草酸激酶;3-脱氢奎尼酸合酶;推定的胞质肽酶 | |
AF145897 | inhA | ||
AF145898 | inhA |
表2(续) | |||
AJ001436 | ectP | ectoine、甜菜碱、脯氨酸的转运 | Peter,H.等“谷氨酸棒杆菌备有四种匹配溶质的第二载体:脯氨酸/ectoine摄入系统ProP和ectoine/脯氨酸/甜菜碱载体EctP的鉴定、测序和特征鉴定”,J.Bacteriol.,180(22):6005-6012(1998) |
AJ004934 | dapD | 四氢2,6-吡啶二羧酸琥珀酰化酶(不完整的i) | Wehrmann,A.等“二氨基庚二酸合成的差别模式及其在细胞壁完整性方面的作用:用谷氨酸棒杆菌的研究”,J.Bacteriol.,180(12):3159-3165(1998) |
AJ007732 | ppc;secG;amt;ocd;soxA | 磷酸烯醇丙酮酸羧化酶;?;高亲合性铵摄入蛋白;推定的鸟氨酸-环脱羧酶;肌氨酸氧化酶 | |
AJ010319 | ftsY,glnB,glnD;srp;amtP | 参与细胞分裂;PH蛋白;尿苷酰基转移酶(尿苷酰基释去酶);信号识别颗粒;低亲合性铵摄入蛋白 | Jakoby,M.等“谷氨酸棒杆菌中的氮调节;涉及相应蛋白质的生物化学鉴定的基因的分离”,FEMS Microbiol.,173(2);303-310(1999) |
AJ132968 | cat | 氯霉素乙酰基转移酶 | |
AJ224946 | mqo | L-苹果酸;醌氧化还原酶 | Molenaar,D.等“得自谷氨酸棒杆菌的膜相关苹果酸脱氢酶(受体)的生物化学和遗传学特征鉴定”,Eur.J.Biochem.,254(2):395-403(1998) |
AJ238250 | ndh | NADH脱氢酶 | |
AJ238703 | porA | 膜孔蛋白 | Lichtinger,T.等“谷氨酸棒杆菌细胞壁膜孔蛋白的生物化学和生物物理学特征鉴定:该通道由低分子量多肽形成”,Biochemistry,37(43):15024-15032(1998) |
D17429 | 转座因子IS31831 | Vertes等“得自谷氨酸棒杆菌的转座因子IS31831的分离和特征鉴定”,Mol.Microbiol.,11(4):739-746(1994) | |
D84102 | odhA | 2-酮戊二酸脱氢酶 | Usuda,Y.等“编码新型2-酮戊二酸脱氢酶的谷氨酸棒杆菌(乳发酵短杆菌AJ12036)odhA基因的分子克隆”,Microbiology,142:3347-3354(1996) |
E01358 | hdh;hk | 高丝氨酸脱氢酶;高丝氨酸激酶 | Katsumata,R.等“L-苏氨酸和L-异亮氨酸的生产”,专利:JP 1987232392-A1 10/12/87 |
E01359 | 高丝氨酸激酶基因起始密码子上游 | Katsumata,R.等“L-苏氨酸和L-异亮氨酸的生产”,专利:JP 1987232392-A 210/12/87 | |
E01375 | 色氨酸操纵子 | ||
E01376 | trpL;trpE | 前导肽;邻氨基苯甲酸合酶 | Matsui,K.等“色氨酸操纵子、由其编码的肽和蛋白质、色氨酸操纵子基因表达的利用和色氨酸的生产”,专利:JP 1987244382-A 1 10/24/87 |
表2(续) | |||
E01377 | 色氨酸操纵子的启动子和操纵基因区 | Matsui,K.等“色氨酸操纵子、由其编码的肽和蛋白质、色氨酸操纵子基因表达的利用和色氨酸的生产”,专利:JP 1987244382-A 1 10/24/87 | |
E03937 | 生物素合酶 | Hatakeyama,K.等“含能够编码生物素合成酶的基因的DNA片段及其应用”,专利:JP 1992278088-A 1 10/02/92 | |
E04040 | 二氨基壬酸氨基转移酶 | Kohama,K.等“编码二氨基壬酸氨基转移酶和脱硫生物素合成酶的基因及其应用”,专利:JP 1992330284-A 1 11/18/92 | |
E04041 | 脱硫生物素合成酶 | Kohama,K.等“编码二氨基壬酸氨基转移酶和脱硫生物素合成酶的基因及其应用”,专利:JP 1992330284-A 1 11/18/92 | |
E04307 | Flavum天冬氨酸酶 | Kurusu,Y.等“编码天冬氨酸酶的基因DNA及其应用”,专利:JP1993030977-A 1 02/09/93 | |
E04376 | 异柠檬酸裂合酶 | Katsumata,R.等“基因表现控制性DNA”,专利:JP 1993056782-A 3 03/09/93 | |
E04377 | 异柠檬酸裂合酶N末端片段 | Katsumata,R.等“基因表现控制性DNA”,专利:JP 1993056782-A 3 03/09/93 | |
E04484 | 预苯酸脱水酶 | Sotouchi,N.等“通过发酵生产L-苯丙氨酸”,专利:JP 1993076352-A 203/30/97 | |
E05108 | 天冬氨酸激酶 | Fugono,N.等“编码天冬氨酸激酶的基因DNA及其应用”,专利:JP1993184366-A 1 07/27/93 | |
E05112 | 二氢-dipichorinate合成酶 | Hatakeyama,K.等“编码二氢2,6-吡啶二羧酸合成酶的基因DNA及其应用”,专利:JP 1993184371-A 1 07/27/93 | |
E05776 | 二氨基庚二酸脱氢酶 | Kobayashi,M.等“编码二氨基庚二酸脱氢酶的基因DNA及其应用”,专利:JP 1993284970-A 1 11/02/93 | |
E05779 | 苏氨酸合酶 | Kohama,K.等“编码苏氨酸合酶的基因DNA及其应用”,专利:JP1993284972-A 1 11/02/93 | |
E06110 | 预苯酸脱水酶 | Kikuchi,T.等“通过发酵法生产L-苯丙氨酸”,专利:JP 1993344881-A 112/27/93 | |
E06111 | 突变型预苯酸脱水酶 | Kikuchi,T.等“通过发酵法生产L-苯丙氨酸”,专利:JP 1993344881-A 112/27/93 | |
E06146 | 乙酰羟酸合成酶 | Inui,M.等“能够编码乙酰羟酸合成酶的基因及其应用”,专利:JP1993344893-A 1 12/27/93 | |
E06825 | 天冬氨酸激酶 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 103/08/94 | |
E06826 | 突变型天冬氨酸激酶α亚基 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 1 |
03/08/94 | |||
表2(续) | |||
E06827 | 突变型天冬氨酸激酶α亚基 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 103/08/94 | |
E07701 | secY | Honno,N.等“参与膜蛋白与膜整合的基因DNA”,专利:JP 1994169780-A 106/21/94 | |
E08177 | 天冬氨酸激酶 | Sato,Y.等“能够编码从反馈抑制释放的天冬氨酸激酶的基因DNA及其应用”,专利:JP 1994261766-A 1 09/20/94 | |
E08178,E08179,E08180,E08181,E08182 | 反馈抑制释放的天冬氨酸激酶 | Sato,Y.等“能够编码从反馈抑制释放的天冬氨酸激酶的基因DNA及其应用”,专利:JP 1994261766-A 1 09/20/94 | |
E08232 | 乙酰羟酸异构还原酶 | Inui,M.等“编码乙酰羟酸异构还原酶的基因DNA”,专利:JP 1994277067-A1 10/04/94 | |
E08234 | secE | Asai,Y.等“编码蛋白质转运机器的基因DNA”,专利:JP 1994277073-A 110/04/94 | |
E08643 | FT氨基转移酶和脱硫生物素合成酶启动子区 | Hatakeyama,K.等“棒状细菌中具有启动子功能的DNA片段”,专利:JP1995031476-A 1 02/03/95 | |
E08646 | 生物素合成酶 | Hatakeyama,K.等“棒状细菌中具有启动子功能的DNA片段”,专利:JP1995031476-A 1 02/03/95 | |
E08649 | 天冬氨酸酶 | Kohama,K.等“棒状细菌中具有启动子功能的DNA片段”,专利:JP1995031478-A 1 02/03/95 | |
E08900 | 二氢2,6-吡啶二羧酸还原酶 | Madori,M.等“含有编码二氢2,6-吡啶二羧酸还原酶的基因的DNA片段及其应用”,专利:JP 1995075578-A 1 03/20/95 | |
E08901 | 二氨基庚二酸脱羧酶 | Madori,M.等“含有编码二氨基庚二酸脱羧酶的基因的DNA片段及其应用”,专利:JP 1995075579-A 1 03/20/95 | |
E12594 | 丝氨酸羟甲基转移酶 | Hatakeyama,K.等“L-色氨酸的生产”,专利:JP 1997028391-A 1 02/04/97 | |
E12760,E12759,E12758 | 转座酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12764 | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12767 | 二氢2,6-吡啶二羧酸合成酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 |
表2(续) | |||
E12770 | 天冬氨酸激酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12773 | 二氢2,6-吡啶二羧酸还原酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E13655 | 葡萄糖-6-磷酸脱氢酶 | Hatakeyama,K.等“葡萄糖-6-磷酸脱氢酶以及能够编码其的DNA”,专利:JP 1997224661-A 1 09/02/97 | |
L01508 | IlvA | 苏氨酸脱水酶 | Moeckel,B.等“谷氨酸棒杆菌苏氨酸脱水酶的功能和结构分析”,J.Bacteriol.,174:8065-8072(1992) |
L07603 | EC 4.2.1.15 | 3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶 | Chen,C.等“谷氨酸棒杆菌3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶基因的克隆和核苷酸序列”,FEMS Microbiol.Lett.,107:223-230(1993) |
L09232 | IlvB;ilvN;ilvC | 乙酰羟酸合酶大亚基;乙酰羟酸合酶小亚基;乙酰羟酸异构还原酶 | Keilhauer,C.等“谷氨酸棒杆菌中的异亮氨酸合成:ilvB-ilvN-ilvC操纵子的分子分析”,J.Bacteriol.,175(17):5595-5603(1993) |
L18874 | PtsM | 磷酸烯醇丙酮酸糖磷酸转移酶 | Fouet,A等“枯草杆菌磷酸转移酶系统的蔗糖特异性酶II:在大肠杆菌中的表达以及与来自肠细菌的酶II的同源性”,PNAS USA,84(24):8773-8777(1987);Lee,J.K.等“编码谷氨酸棒杆菌甘露糖酶II的基因的核苷酸序列和推导的蛋白质序列的分析”,FEMS Microbiol.Lett.,119(1-2):137-145(1994) |
L27123 | aceB | 苹果酸合酶 | Lee,H-S.等“aceB-一种谷氨酸棒杆菌中编码苹果酸合酶的基因的分子特征鉴定”,J.Microbiol.Biotechnol.,4(4):256-263(1994) |
L27126 | 丙酮酸激酶 | Jetten,M.S.等“来自谷氨酸棒杆菌的丙酮酸激酶的结构与功能分析”,Appl.Environ.Microbiol.,60(7):2501-2507(1994) | |
L28760 | aceA | 异柠檬酸裂合酶 | |
L35906 | dtxr | 白喉毒素阻抑蛋白 | Oguiza,J.A.等“来自乳发酵短杆菌的白喉棒杆菌dtxR的分子克隆、DNA序列分析和特征鉴定”,J.Bacteriol.,177(2):465-467(1995) |
M13774 | 预苯酸脱水酶 | Follettie,M.T.等“谷氨酸棒杆菌pheA基因的分子克隆和核苷酸序列”,J.Bacteriol.,167:695-702(1986) | |
M16175 | 5S rRNA | Park,Y-H.等“通过56种rRNA序列对棒状细菌进行的种系发生分析”,J.Bacteriol.,169:1801-1806(1987) | |
M16663 | trpE | 邻氨基苯甲酸合酶,5’端 | Sano,K.等“一种谷氨酸生产细菌-乳发酵短杆菌trp操纵子控制区的结构和功能”,Gene,52:191-200(1987) |
M16664 | trpA | 色氨酸合酶,3’端 | Sano,K.等“一种谷氨酸生产细菌-乳发酵短杆菌trp操纵子控制区的结构和功能”,Gene,52:191-200(1987) |
表2(续) | |||
M25819 | 磷酸烯醇丙酮酸羧化酶 | O’Regan,M.等“谷氨酸棒杆菌ATCC13032的磷酸烯醇丙酮酸羧化酶编码基因的克隆和核苷酸序列”,Gene,77(2):237-251(1989) | |
M85106 | 23S rRNA基因插入序列 | Roller,C.等“具有高DNAG+C含量的革兰氏阳性细菌的特征为其23SrRNA基因内的一个共同插入”,J.Gen.Microbiol.,138:1167-1175(1992) | |
M85107,M85108 | 23S rRNA基因插入序列 | Roller,C.等“具有高DNAG+C含量的革兰氏阳性细菌的特征为其23SrRNA基因内的一个共同插入”,J.Gen.Microbiol.,138:1167-1175(1992) | |
M89931 | aecD;brnQ;yhbw | βC-S裂合酶;支链氨基酸摄入载体;假定的蛋白yhbw | Rossol,I.等“谷氨酸棒杆菌aecD基因编码一种具有降解氨乙基半胱氨酸的α,β-消除活性的C-S裂合酶”,J.Bacteriol.174(9):2968-2977(1992);Tauch,A.等“谷氨酸棒杆菌ATCC 13032中的异亮氨酸摄入由brnQ基因产物指导”,Arch.Microbiol.,169(4):303-312(1998) |
S59299 | trp | 前导基因(启动子) | Herry,D.M.等“来自谷氨酸棒杆菌色氨酸高生产菌株的trp基因簇的克隆:trp前导序列中一个突变的鉴定”,Appl.Environ.Microbiol.,59(3):791-799(1993) |
U11545 | trpD | 邻氨基苯甲酸磷酸核糖基转移酶 | O’Gara,J.P.和Dunican,L.K.(1994)“谷氨酸棒杆菌ATCC 21850 tpD基因的完整核苷酸序列”,论文,Microbiology Department,University College Galway,Ireland |
U13922 | cglIM;cglIR;clgIIR | 推定的II型5-胞嘧啶甲基转移酶;推定的II型限制性内切核酸酶;推定的I型或III型限制性内切核酸酶 | Schafer,A.等“来自谷氨酸棒杆菌ATCC 13032的编码胁迫敏感性限制系统的DNA区的克隆和特征鉴定及其在与大肠杆菌的属间接合方面的作用分析”,J.Bacterol.176(23):7309-7319(1994);Schafer,A.等“谷氨酸棒杆菌编码McrBC缺陷型大肠杆菌菌株中的5-胞嘧啶的cglIM基因”,Gene,203(2):95-101(1997) |
U14965 | recA | ||
U31224 | ppx | Ankri,S.等“谷氨酸棒杆菌脯氨酸生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) | |
U31225 | proC | L-脯氨酸:NADP+5-氧化还原酶 | Ankri,S.等“谷氨酸棒杆菌脯氨酸生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) |
U31230 | obg;proB;unkdh | ?;γ-谷氨酰激酶;与D-异构体特异性的2-羟酸脱氢酶相似 | Ankri,S.等“谷氨酸棒杆菌脯氨酸生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) |
表2(续) | |||
U31281 | bioB | 生物素合酶 | Serebriiskii,I.G.,“bioB超家族的两个新成员:Methylobacillus flagellatum和谷氨酸棒杆菌的bio B基因的克隆、测序和表达”,Gene,175:15-22(1996) |
U35023 | thtR;accBC | 硫代硫酸硫转移酶;酰基CoA羧化酶 | Jager,W.等“编码类似于生物素羧化酶和生物素羧基载体蛋白的二结构域蛋白的谷氨酸棒杆菌基因”,Arch.Microbiol.,166(2);76-82(1996) |
U43535 | cmr | 多种药物抗性蛋白 | Jager,W.等“在异源宿主大肠杆菌中赋予多种药物抗性的谷氨酸棒杆菌基因”,J.Bacteriol.,179(7):2449-2451(1997) |
U43536 | clpB | 热激ATP结合蛋白 | |
U53587 | aphA-3 | 3’5”-氨基糖苷磷酸转移酶 | |
U89648 | 谷氨酸棒杆菌中参与组氨酸生物合成的未鉴定序列、部分序列 | ||
X04960 | trpA;trpB;trpC;trpD;TrpE;trpG;trpL | 色氨酸操纵子 | Matsui,K.等“乳发酵短杆菌色氨酸操纵子的完整核苷酸序列和推导的氨基酸序列”,Nucleic Acids Res.,14(24):10113-10114(1986) |
X07563 | lys A | DAP脱羧酶(中-二氨基庚二酸脱羧酶,EC 4.1.1.20) | Yeh,P.等“谷氨酸棒杆菌的lysA基因的核酸序列以及其表达调节的可能机制”,Mol.Gen.Genet.,212(1):112-119(1988) |
X14234 | EC 4.1.1.31 | 磷酸烯醇丙酮酸羧化酶 | Eikmanns,B.J.等“谷氨酸棒杆菌的磷酸烯醇丙酮酸羧化酶基因:分子克隆、核苷酸序列和表达”,Mol.Gen.Genet.,218(2):330-339(1989);Lepiniec,L.等“高粱磷酸烯醇丙酮酸羧化酶基因家族:结构、功能和分子进化”,Plant.Mol.Biol.,21(3):487-502(1993) |
X17313 | fda | 果糖二磷酸醛缩酶 | Von der Osten,C.H.等“谷氨酸棒杆菌fda基因的分子克隆、核苷酸序列和精细结构分析:谷氨酸棒杆菌果糖-1,6-二磷酸醛缩酶与I类和II类醛缩酶的结构比较”,Mol.Microbiol., |
X53993 | dapA | L-2,3-二氢2,6-吡啶二羧酸合成酶(EC 4.2.1.52) | Bonnassie,S.等“来自谷氨酸棒杆菌的dapA基因的核酸序列”,Nucleic AcidsRes.,18(21):6421(1990) |
X54223 | AttB相关位点 | Cianciotto,N.等“白喉棒杆菌、溃疡棒杆菌(Corynebacterium ulcerans)和谷氨酸棒杆菌att B相关位点和λ棒杆菌噬菌体的attP位点之间的DNA序列同源性”,FEMS,Microbiol,Lett.,66:299-302(1990) | |
X54740 | argS;lysA | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶 | Marcel,T.等“谷氨酸棒杆菌lysA基因上游区的核苷酸序列和组构”,Mol.Microbiol.,4(11):1819-1830(1990) |
表2(续) | |||
X55994 | trpL;trpE | 推定的前导肽;邻氨基苯甲酸合酶组分I | Heery,D.M.等“谷氨酸棒杆菌trpE基因的核苷酸序列”,Nucleic Acids Res.,18(23):7138(1990) |
X56037 | thrC | 苏氨酸合酶 | Han,K.S.等“谷氨酸棒杆菌苏氨酸合酶基因的分子结构”,Mol.Microbiol.,4(10):1693-1702(1990) |
X56075 | attB-相关位点 | 附着位点 | Cianciotto,N.等“白喉棒杆菌、溃疡棒杆菌(Corynebacterium ulcerans)和谷氨酸棒杆菌att B相关位点和λ棒杆菌噬菌体的attP位点之间的DNA序列同源性”,FEMS.Microbiol,Lett.,66:299-302(1990) |
X57226 | lysC-α;lysC-β;asd | 天冬氨酸激酶α亚基;天冬氨酸激酶β亚基;天冬氨酸β半醛脱氢酶 | Kalinowski,J.等“来自谷氨酸棒杆菌的天冬氨酸激酶的遗传学和生物化学分析”,Mol.Microbiol.,5(5):1197-1204(1991);Kalinowski,J.等“天冬氨酸激酶基因lysCα和lysCβ重叠并且在谷氨酸棒杆菌中与天冬氨酸β半醛脱氢酶基因asd相邻”,Mol.Gen.Genet.,224(3):317-324(1990) |
X59403 | gap;pgk;tpi | 甘油醛-3-磷酸;磷酸甘油酸激酶;丙糖磷酸异构酶 | Eikmanns,B.J.“一种编码三种糖酵解酶甘油醛-3-磷酸脱氢酶、磷酸甘油酸激酶和丙糖磷酸异构酶的谷氨酸棒杆菌基因簇的鉴定、序列分析和表达”,J.Bacteriol.,174(19):6076-6086(1992) |
X59404 | gdh | 谷氨酸脱氢酶 | Bormann,E.R.等“编码谷氨酸脱氢酶的谷氨酸棒杆菌gdh基因的分子分析”,Mol.Microbiol.,6(3):317-326(1992) |
X60312 | Lysl | L-赖氨酸通透酶 | Seep-Feldhaus,A.H.等“参与赖氨酸摄入的谷氨酸棒杆菌lysI基因的分子分析”,Mol.Microbiol.,5(12):2995-3005(1991) |
X66078 | Copl | Psl蛋白 | Joliff,G.等“编码谷氨酸棒杆菌两种主要分泌蛋白之一PS1的csp 1基因的克隆和核苷酸序列:PS1的推导的N末端区与分枝杆菌属抗原85复合体相似”,Mol.Microbiol.,6(16):2349-2362(1992) |
X66112 | glt | 柠檬酸合酶 | Eikmanns,B.J.等“编码柠檬酸合酶的谷氨酸棒杆菌gltA基因的克隆序列、表达和转录分析”,Microbiol.,140:1817-1828(1994) |
X67737 | dapB | 二氢2,6-吡啶二羧酸还原酶 | |
X69103 | csp2 | 表层蛋白PS2 | Peyret,J.L.等“编码谷氨酸棒杆菌中一种有序表层蛋白PS2的cspB基因的特征鉴定”,Mol.Microbiol.,9(1):97-109(1993) |
X69104 | IS3相关插入元件 | Bonamy,C.等“一种谷氨酸棒杆菌IS3相关插入序列IS 1206的鉴定以及种系发生分析”,Mol.Microbiol.,14(3):571-581(1994) |
表2(续) | |||
X70959 | leuA | 异丙基苹果酸合酶 | Patek,M.等“谷氨酸棒杆菌中的亮氨酸合成:leuA的酶活性、结构以及leuA失活对赖氨酸合成的影响”,Appl.Environ.Microbiol.,60(1):133-140(1994) |
X71489 | icd | 异柠檬酸脱氢酶(NADP+) | Eikmanns,B.J.等“编码异柠檬酸脱氢酶的谷氨酸棒杆菌icd基因的克隆序列分析、表达和失活以及该酶的生化特征鉴定”,J.Bacteriol.,177(3):774-782(1995) |
X72855 | GDHA | 谷氨酸脱氢酶(NADP+) | |
X75083,X70584 | mtrA | 5-甲基色氨酸抗性 | Heery,D.M.等“来自谷氨酸棒杆菌色氨酸高生产菌株编码5-甲基色氨酸抗性的序列”,Biochem.Biophys.Res.Commun.,201(3):1255-1262(1994) |
X75085 | recA | Fitzpatrick,R.等“谷氨酸棒杆菌和乳发酵短杆菌的recA突变株的构建和特征鉴定”,Appl.Microbiol.Biotechnol.,42(4):575-580(1994) | |
X75504 | aceA;thiX | 部分异柠檬酸裂合酶;? | Reinscheid,D.J.等“来自谷氨酸棒杆菌的异柠檬酸裂合酶基因的特征鉴定以及该酶的生化分析”,J.Bacteriol.,176(12):3474-3483(1994) |
X76875 | ATP酶β亚基 | Ludwig,W.等“基于延伸因子Tu和ATP合酶β亚基基因比较序列分析的细菌种系发生关系”,Antonie Van Leeuwenhoek,64:285-305(1993) | |
X77034 | tuf | 延伸因子Tu | Ludwig,W.等“基于延伸因子Tu和ATP合酶β亚基基因比较序列分析的细菌种系发生关系”,Antonie Van Leeuwenhoek,64:285-305(1993) |
X77384 | recA | Billman-Jacobe,H.“来自谷氨酸棒杆菌的recA基因的核苷酸序列”,DNASeq.,4(6):403-404(1994) | |
X78491 | aceB | 苹果酸合酶 | Reinscheid,D.J.等“来自编码磷酸转乙酰基酶的谷氨酸棒杆菌pta-ack操纵子的苹果酸合酶:序列分析”,Microbiology,140:3099-3108(1994) |
X80629 | 16S rDNA | 16S核糖体RNA | Rainey,F.A.等“红球菌属(Rhodococcus)和诺卡氏菌属(Norcardia)的种系发生分析和诺卡氏菌属来自红球菌辐射的进化起源的证据”,Microbiol.,141:523-528(1995) |
X81191 | gluA;gluB;gluC;gluD | 谷氨酸摄入系统 | Kronemeyer,W.等“编码谷氨酸棒杆菌谷氨酸摄入系统的gluABCD簇的结构”,J.Bacteriol.,177(5):1152-1158(1995) |
X81379 | dapE | 琥珀酰二氨基庚二酸脱琥珀酰基酶 | Wehrmann,A.等“互补大肠杆菌dapE的谷氨酸棒杆菌的不同DNA片段的分析”,Microbiology,40:3349-56(1994) |
表2(续) | |||
X82061 | 16S rDNA | 16S核糖体RNA | Ruimy,R.等“由小亚基核糖体DNA序列分析推导的棒杆菌属的种系发生”,Int.J.Syst.Bacteriol.,45(4):740-746(1995) |
X82928 | asd;lysC | 天冬氨酸半醛脱氢酶;? | Serebrijski,I.等“proA突变体中由asd基因引起的多拷贝抑制和由异源proA引起的渗透胁迫依赖性互补”,J.Bacteriol.,177(24):7255-7260(1995) |
X82929 | proA | γ-谷氨酰磷酸还原酶 | Serebrijski,I.等“proA突变体中由asd基因引起的多拷贝抑制和由异源proA引起的渗透胁迫依赖性互补”,J.Bacteriol.,177(24):7255-7260(1995) |
X84257 | 16S rDNA | 16S核糖体RNA | Pascual,C.等“基于16S rRNA基因序列的棒杆菌属的种系发生分析”,Int.J.Syst.Bacteriol,45(4):724-728(1995) |
X85965 | aroP;dapE | 芳族氨基酸通透酶;? | Wehrmann等“谷氨酸棒杆菌脯氨酸dapE的相邻序列的功能分析揭示编码芳族氨基酸转运蛋白的arop的存在”,J.Bacteriol.,177(20):5991-5993(1995) |
X86157 | argB;argC;argD;argF;argJ | 乙酰谷氨酸激酶;N-乙酰-γ-谷氨酰磷酸还原酶;乙酰鸟氨酸氨基转移酶;鸟氨酸氨甲酰转移酶;谷氨酸N-乙酰基转移酶 | Sakanyan,V.等“谷氨酸棒杆菌中精氨酸生物合成乙酰基循环的基因和酶:精氨酸途径早期步骤中的酶进化”,Microbiology,142:99-108(1996) |
X89084 | pta;ackA | 磷酸乙酰基转移酶;乙酸激酶 | Reinscheid,D.J.等“编码磷酸转乙酰基酶和乙酸激酶的谷氨酸棒杆菌pta-ack操纵子的克隆、序列分析、表达和失活”,Microbiology,145:503-513(1999) |
X89850 | attB | 附着位点 | Le Marrec,C.等“感染“金黄节杆菌(Arthrobacter aureus)C70”的phi AAU2定点整合功能的遗传特征鉴定”,J.Bacteriol.,178(7):1996-2004(1996) |
X90356 | 启动子片段F1 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90357 | 启动子片段F2 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90358 | 启动子片段F10 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90359 | 启动子片段F13 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) |
表2(续) | |||
X90360 | 启动子片段F22 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90361 | 启动子片段F34 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90362 | 启动子片段F37 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90363 | 启动子片段F45 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90364 | 启动子片段F64 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90365 | 启动子片段F75 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90366 | 启动子片段PF101 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90367 | 启动子片段PF104 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90368 | 启动子片段PF109 | Patek,M.等“来自谷氨酸棒杆菌的启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X93513 | amt | 铵转运系统 | Siewe,R.M.等“谷氨酸棒杆菌(甲基)铵摄入载体的功能和遗传特征鉴定”,J.Biol.Chem.,271(10):5398-5403(1996) |
X93514 | betP | 甜菜碱转运系统 | Peter,H.等“编码匹配溶质甜菜碱转运系统的谷氨酸棒杆菌betP基因分离、特征鉴定和表达”,J.Bacteriol,178(17):5229-5234(1996) |
X95649 | orf4 | Patek,M.等“编码参与L-赖氨酸合成的两种酶的谷氨酸棒杆菌dapB-ORF2-dapA-ORF4操纵子的鉴定和转录分析”,Biotechnol.Lett.,19:1113-1117(1997) | |
X96471 | lysE;lysG | 赖氨酸输出蛋白;赖氨酸输出调节蛋白 | Vrljic,M.等“具有新类型细胞功能的新类型转运蛋白:来自谷氨酸棒杆菌的L-赖氨酸输出”,Mol.Microbiol.,22(5):815-826(1996) |
表2(续) | |||
X96580 | panB;panC;xylB | 3-甲基-2-酮丁酸羟甲基转移酶;泛解酸-β-丙氨酸连接酶;木酮糖激酶 | Sahm,H.等“谷氨酸棒杆菌中的D-泛酸合成以及将panBC和编码L-缬氨酸合成的基因应用于D-泛酸的过量生产”,Appl.Environ.Microbiol.,65(5):1973-1979(1999) |
X96962 | 插入序列IS1207和转座酶 | ||
X99289 | 延伸因子P | Ramos,A.等“编码氨基酸生产菌乳发酵短杆菌(谷氨酸棒杆菌ATCC 13869)中延伸因子P的基因的克隆、测序和表达”,Gene,198:217-222(1997) | |
Y00140 | thrB | 高丝氨酸激酶 | Mateos,L.M.等“乳发酵短杆菌的高丝氨酸激酶(thrB)基因的核苷酸序列”,Nucleic Acids Res.,15(9):3922(1987) |
Y00151 | ddh | 中-二氨基庚二酸D-脱氢酶(EC 1.4.1.16) | Ishino,S.等“来自谷氨酸棒杆菌的中-二氨基庚二酸D-脱氢酶基因的核苷酸序列”,Nucleic Acids Res.,15(9):3917(1987) |
Y00476 | thrA | 高丝氨酸脱氢酶 | Mateos,L.M.等“乳发酵短杆菌的高丝氨酸脱氢酶(thrA)基因的核苷酸序列”,Nucleic Acids Res.,15(24):10598(1987) |
Y00546 | hom;thrB | 高丝氨酸脱氢酶;高丝氨酸激酶 | Peoples,O.P.等“谷氨酸棒杆菌hom-thrB操纵子的核苷酸序列和精细结构分析”,Mol.Microbiol.,2(1):63-72(1988) |
Y08964 | murC;ftsQ/divD;ftsZ | UPD-N-乙酰胞壁酸-丙氨酸连接酶;分裂起始蛋白或细胞分裂蛋白;细胞分裂蛋白 | Honrubia,M.P.等“来自乳发酵短杆菌的ftsZ基因的鉴定、特征鉴定和染色体组构”,Mol.Gen.Genet.,259(1):97-104(1998) |
Y09163 | putP | 高亲合性脯氨酸转运系统 | Peter,H.等“谷氨酸棒杆菌脯氨酸的putP基因的分离和匹配溶质的低亲合性摄入系统的特征鉴定”,Arch.Microbiol.,168(2):143-151(1997) |
Y09548 | pyc | 丙酮酸羧化酶 | Peters-Wendisch,P.G.等“来自谷氨酸棒杆菌的丙酮酸羧化酶:pyc基因的特征鉴定、表达和失活”,Microbiology,144:915-927(1998) |
Y09578 | leuB | 3-异丙基苹果酸脱氢酶 | Patek,M.等“来自谷氨酸棒杆菌的leuB基因的分析”,Appl.Microbiol,Biotechnol.,50(1):42-47(1998) |
Y12472 | 附着位点噬菌体Phi-16 | Moreau,S.等“棒杆菌噬菌体Phi-16的定点整合:整合载体的构建”,Microbiol.,145:539-548(1999) | |
Y12537 | proP | 脯氨酸/ectoine摄入系统蛋白 | Peter,H.等“谷氨酸棒杆菌备有四种匹配溶质的第二载体:脯氨酸/ectoine摄入系统ProP以及ectoine/脯氨酸/甜菜碱载体EctP的鉴定、测序和特征鉴定”,J.Bacteriol.,180(22):6005-6012(1998) |
表2(续) | |||
Y13221 | glnA | 谷氨酰胺合成酶I | Jakoby,M.等“编码谷氨酰胺合成酶I的谷氨酸棒杆菌glnA基因的分离”,FEMS Microbiol.Lett.,154(1):81-88(1997) |
Y16642 | lpd | 二氢硫辛酰胺脱氢酶 | |
Y18059 | 附着位点棒杆菌噬菌体304L | Moreau,S.等“&phi;304L整合功能的分析:棒杆菌噬菌体中的一种整合酶组件”,Virology,255(1):150-159(1999) | |
Z21501 | argS;lysA | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶(部分) | Oguiza,J.A.等“编码精氨酰-tRNA合成酶的基因位于乳发酵短杆菌lysA基因的上游区中:通过精氨酸调节argS-lysA簇表达”,J.Bacteriol.,175(22):7356-7362(1993) |
Z21502 | dapA;dapB | 二氢2,6-吡啶二羧酸合酶;二氢2,6-吡啶二羧酸还原酶 | Pisabarro,A.等“乳发酵短杆菌三种基因(dapA、orf2和dapB)的基因簇编码二氢2,6-吡啶二羧酸还原酶和一种功能未知的第三种多肽”,J.Bacteriol.,175(9):2743-2749(1993) |
Z29563 | thrC | 苏氨酸合酶 | Malumbres,M.等“所编码苏氨酸合酶的thrC基因的分析和表达”,Appl.Environ.Microbiol.,60(7)2209-2219(1994) |
ZA6753 | 16S rDNA | 16S核糖体RNA的基因 | |
ZA9822 | sigA | SigAσ因子 | Oguiza,J.A.等“乳发酵短杆菌中的多个σ因子基因:sigA和sigB的特征鉴定”,J.Bacteriol.,178(2):550-553(1996) |
Z49823 | galE;dtxR | 催化活性UDP-半乳糖4-差向异构酶;白喉毒素调节蛋白 | Oguiza,J.A.等“乳发酵短杆菌编码UDP-半乳糖4-差向异构酶的galE基因与dmdR基因转录偶联”,Gene,177:103-107(1996) |
Z49824 | orfI;sigB | ?;SigBσ因子 | Oguiza,J.A.等“乳发酵短杆菌中的多个σ因子基因:sigA和sigB的特征鉴定”,J.Bacteriol.,178(2):550-553(1996) |
Z66534 | 转座酶 | Correia,A.等“存在于乳发酵短杆菌ATCC 13869基因组中的IS样元件的克隆和特征鉴定”,Gene,170(1):91-94(1996) | |
1该基因的序列公布于所指明的参考文献中。然而,本申请的发明人获得的序列明显长于所公布的形式。认为所公布的形式依赖于不正确的起始密码子,因此仅代表真实编码区的片段。 |
表3:可以用于实施本发明的棒杆菌属和短杆菌属菌株
菌种 | ATCC | FERM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
产氨短杆菌(Brevibacterium ammoniagenes) | 21054 | |||||||
产氨短杆菌 | 19350 | |||||||
产氨短杆菌 | 19351 | |||||||
产氨短杆菌 | 19352 | |||||||
产氨短杆菌 | 19353 | |||||||
产氨短杆菌 | 19354 | |||||||
产氨短杆菌 | 19355 | |||||||
产氨短杆菌 | 19356 | |||||||
产氨短杆菌 | 21055 | |||||||
产氨短杆菌 | 21077 | |||||||
产氨短杆菌 | 21553 | |||||||
产氨短杆菌 | 21580 | |||||||
产氨短杆菌 | 39101 | |||||||
Brevibacterium butanicum | 21196 | |||||||
谷氨酸棒杆菌(Brevibacterium divaricatum) | 21792 | P928 | ||||||
黄色短杆菌(Brevibacterium flavum) | 21474 | |||||||
黄色短杆菌 | 21129 | |||||||
黄色短杆菌 | 21518 | |||||||
黄色短杆菌 | B11474 | |||||||
黄色短杆菌 | B11472 | |||||||
黄色短杆菌 | 21127 | |||||||
黄色短杆菌 | 21128 | |||||||
黄色短杆菌 | 21427 | |||||||
黄色短杆菌 | 21475 | |||||||
黄色短杆菌 | 21517 | |||||||
黄色短杆菌 | 21528 | |||||||
黄色短杆菌 | 21529 | |||||||
黄色短杆菌 | B11477 | |||||||
黄色短杆菌 | B11478 | |||||||
黄色短杆菌 | 21127 | |||||||
黄色短杆菌 | B11474 | |||||||
希氏短杆菌(Brevibacterium healii) | 15527 | |||||||
酮戊二酸短杆菌(Brevibacterium ketoglutamicum) | 21004 | |||||||
酮戊二酸短杆菌 | 21089 | |||||||
酮戊二酸短杆菌 | 21914 | |||||||
乳发酵短杆菌 | 70 | |||||||
乳发酵短杆菌 | 74 | |||||||
乳发酵短杆菌 | 77 | |||||||
乳发酵短杆菌 | 21798 | |||||||
乳发酵短杆菌 | 21799 | |||||||
乳发酵短杆菌 | 21800 | |||||||
乳发酵短杆菌 | 21801 | |||||||
乳发酵短杆菌 | B11470 | |||||||
乳发酵短杆菌 | B11471 | |||||||
乳发酵短杆菌 | 21086 | |||||||
乳发酵短杆菌 | 21420 | |||||||
乳发酵短杆菌 | 21086 |
乳发酵短杆菌 | 31269 | |||||||
扩展短杆菌(Brevibacterium linens) | 9174 | |||||||
扩展短杆菌 | 19391 | |||||||
扩展短杆菌 | 8377 | |||||||
Brevibacterium paraffinolyticum | 11160 | |||||||
短杆菌(Brevibacterium spec.) | 717.73 | |||||||
短杆菌 | 717.73 | |||||||
短杆菌 | 14604 | |||||||
短杆菌 | 21860 | |||||||
短杆菌 | 21864 | |||||||
短杆菌 | 21865 | |||||||
短杆菌 | 21866 | |||||||
短杆菌 | 19240 | |||||||
嗜乙酰乙酸棒杆菌(Corynebacterium acetoacidophilum) | 21476 | |||||||
嗜乙酰乙酸棒杆菌 | 13870 | |||||||
醋谷棒杆菌(Corynebacteriumacetoglutamiphilum) | B11473 | |||||||
醋谷棒杆菌 | B11475 | |||||||
醋谷棒杆菌 | 15806 | |||||||
醋谷棒杆菌 | 21491 | |||||||
醋谷棒杆菌 | 31270 | |||||||
嗜乙酰棒杆菌(Corynebacterium acetophilum) | B3671 | |||||||
产氨棒杆菌 | 6872 | 2399 | ||||||
产氨棒杆菌 | 15511 | |||||||
Corynebacterium fujiokense | 21496 | |||||||
谷氨酸棒杆菌 | 14067 | |||||||
谷氨酸棒杆菌 | 39137 | |||||||
谷氨酸棒杆菌 | 21254 | |||||||
谷氨酸棒杆菌 | 21255 | |||||||
谷氨酸棒杆菌 | 31830 | |||||||
谷氨酸棒杆菌 | 13032 | |||||||
谷氨酸棒杆菌 | 14305 | |||||||
谷氨酸棒杆菌 | 15455 | |||||||
谷氨酸棒杆菌 | 13058 | |||||||
谷氨酸棒杆菌 | 13059 | |||||||
谷氨酸棒杆菌 | 13060 | |||||||
谷氨酸棒杆菌 | 21492 | |||||||
谷氨酸棒杆菌 | 21513 | |||||||
谷氨酸棒杆菌 | 21526 | |||||||
谷氨酸棒杆菌 | 21543 | |||||||
谷氨酸棒杆菌 | 13287 | |||||||
谷氨酸棒杆菌 | 21851 | |||||||
谷氨酸棒杆菌 | 21253 | |||||||
谷氨酸棒杆菌 | 21514 | |||||||
谷氨酸棒杆菌 | 21516 | |||||||
谷氨酸棒杆菌 | 21299 | |||||||
谷氨酸棒杆菌 | 21300 | |||||||
谷氨酸棒杆菌 | 39684 | |||||||
谷氨酸棒杆菌 | 21488 | |||||||
谷氨酸棒杆菌 | 21649 |
谷氨酸棒杆菌 | 21650 | |||||||
谷氨酸棒杆菌 | 19223 | |||||||
谷氨酸棒杆菌 | 13869 | |||||||
谷氨酸棒杆菌 | 21157 | |||||||
谷氨酸棒杆菌 | 21158 | |||||||
谷氨酸棒杆菌 | 21159 | |||||||
谷氨酸棒杆菌 | 21355 | |||||||
谷氨酸棒杆菌 | 31808 | |||||||
谷氨酸棒杆菌 | 21674 | |||||||
谷氨酸棒杆菌 | 21562 | |||||||
谷氨酸棒杆菌 | 21563 | |||||||
谷氨酸棒杆菌 | 21564 | |||||||
谷氨酸棒杆菌 | 21565 | |||||||
谷氨酸棒杆菌 | 21566 | |||||||
谷氨酸棒杆菌 | 21567 | |||||||
谷氨酸棒杆菌 | 21568 | |||||||
谷氨酸棒杆菌 | 21569 | |||||||
谷氨酸棒杆菌 | 21570 | |||||||
谷氨酸棒杆菌 | 21571 | |||||||
谷氨酸棒杆菌 | 21572 | |||||||
谷氨酸棒杆菌 | 21573 | |||||||
谷氨酸棒杆菌 | 21579 | |||||||
谷氨酸棒杆菌 | 19049 | |||||||
谷氨酸棒杆菌 | 19050 | |||||||
谷氨酸棒杆菌 | 19051 | |||||||
谷氨酸棒杆菌 | 19052 | |||||||
谷氨酸棒杆菌 | 19053 | |||||||
谷氨酸棒杆菌 | 19054 | |||||||
谷氨酸棒杆菌 | 19055 | |||||||
谷氨酸棒杆菌 | 19056 | |||||||
谷氨酸棒杆菌 | 19057 | |||||||
谷氨酸棒杆菌 | 19058 | |||||||
谷氨酸棒杆菌 | 19059 | |||||||
谷氨酸棒杆菌 | 19060 | |||||||
谷氨酸棒杆菌 | 19185 | |||||||
谷氨酸棒杆菌 | 13286 | |||||||
谷氨酸棒杆菌 | 21515 | |||||||
谷氨酸棒杆菌 | 21527 | |||||||
谷氨酸棒杆菌 | 21544 | |||||||
谷氨酸棒杆菌 | 21492 | |||||||
谷氨酸棒杆菌 | B8183 | |||||||
谷氨酸棒杆菌 | B8182 | |||||||
谷氨酸棒杆菌 | B12416 | |||||||
谷氨酸棒杆菌 | B12417 | |||||||
谷氨酸棒杆菌 | B12418 | |||||||
谷氨酸棒杆菌 | B11476 | |||||||
谷氨酸棒杆菌 | 21608 | |||||||
百合花棒杆菌(Corynebacterium lilium) | P973 | |||||||
Corynebacterium nitrilophilus | 21419 | 11594 | ||||||
棒杆菌(Corynebacterium spec.) | P4445 | |||||||
棒杆菌 | P4446 | |||||||
棒杆菌 | 31088 |
棒杆菌 | 31089 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 15954 | 20145 | ||||||
棒杆菌 | 21857 | |||||||
棒杆菌 | 21862 | |||||||
棒杆菌 | 21863 |
ATCC:美国典型培养物保藏中心,Rockville,MD,USA
FERM:发酵研究所,Chiba,日本
NRRL:农业研究机构培养物保藏中心,Northern Regional ResearchLaboratory,Peoria,IL,USA
CECT:西班牙典型培养物保藏中心,Valencia,西班牙
NCIMB:国立工业和海洋微生物保藏有限公司,Aberdeen,英国
CBS:真菌菌种保藏中心,Baarn,荷兰
NCTC:国立典型培养物保藏中心,London,英国
DSMZ:德意志微生物保藏中心,Braunschweig,德国
有关参考文献参见Sugawara,H.等(1993)World directory of collections ofcultures of microorganisms:Bacteria,fungi and yeasts(第4版),World federation forculture collections world data center on microorganisms,Saimata,Japan。
表4:序列比对结果ID# 长度 Genbank命中 长度 登记号 Genbank命中的名称 Genbank命中 同源性% 录入日期
(NT) 的来源 (GAP)rxa00026 1509 GB_RO:MMHC310M6 158405 AF109906 小家鼠MHC类III区RD基因,部分编码区;Rf、C2、G9A、NG22、 小家鼠 38,003 1998-12-10
G9、HSP70、HSP70、HSC70t和smRNP基因,完整编码区;G7A (Mus musculus)
基因,部分编码区;和未知基因
GB_HTG2:AC007029 119007 AC007029 人克隆DJ0855F16,***测序正在进行之中***,1个非顺序片段。 人(Homo sapiens) 37,943 99-04-07
GB_HTG2:AC007029 119007 AC007029 人克隆DJ0855F16,***测序正在进行之中***,1个非顺序片段。 人 37,943 99-04-07rxa00072rxa00111 1116 GB_BA1:SAUSIGA 2748 M94370 橙色标桩菌σ因子(sigA)基因,完整编码区。 橙色标桩菌 40,435 94-08-16
(Stigmatella aurantiaca)
GB_BA1:SC5B8 28500 AL022374 天蓝色链霉菌粘粒5B8。 天蓝色链霉菌 40,090 98-04-22
(Streptomyces coelicolor)
GB_BA2:AE001767 9086 AE001767 海栖热袍菌完整基因组的区域79/136(section 79 of 136) 海栖热袍菌(Thermotoga maritima) 35,091 99-06-02rxa00112 1314 GB_EST35:AU075536 418 AU075536 AU075536水稻苗稻cDNA克隆S0028_2Z,mRNA序列。 稻(Oryza sativa) 39,423 99-07-07
GB_GSS9:AQ157585 647 AQ157585 nbxb0009B 16r CUGI水稻BAC文库稻基因组克隆nbxb0009B 16r, 稻 40,867 98-09-12
基因组综述序列。
GB_GSS14:AQ510314 542 AQ510314 nbxb0095O05f CUGI水稻BAC文库稻基因组克隆nbxb0095O05f, 稻 39,372 1999-05-04
基因组综述序列。rxa00133 936 GB_BA1:SC2G5 38404 AL035478 天蓝色链霉菌粘粒2G5。 天蓝色链霉菌 41,170 99-06-11
GB_EST7:W64291 515 W64291 md98h12.r1 Soares小鼠胚胎NbME13.5 14.5小家鼠cDNA克隆 小家鼠 35,306 96-06-10
IMAGE:386087类似于gb:L26528小家鼠Rab11b mRNA的5’,完
整编码区(小鼠);mRNA序列。
GB_PR3:AC005624 39594 AC005624 人19号染色体,粘粒R30017,完整序列。 人 39,054 98-09-06rxa00137 1212 GB_BA2:AF124600 4115 AF124600 谷氨酸棒杆菌分支酸合酶(aroC)、莽草酸激酶(aroK)和3-脱氢奎尼 谷氨酸棒杆菌 99,867 1999-05-04
酸合酶(aroB)基因,完整编码区;和推定的胞质肽酶(pepQ)基因,
部分编码区。
GB_BA1:MTCY159 33818 Z83863 结核分枝杆菌H37Rv完整基因组;区段111/162。 结核分枝杆菌 40,959 98-06-17
(Mycobacterium tuberculosis)
GB_BA1:MT3DEHQ 3437 X59509 结核分枝杆菌,3-脱氢奎尼酸合酶和3-dehydroquinase的基因。 结核分枝杆菌 52,583 93-06-30rxa00139 834 GB_BA1:BLELONP 738 X99289 乳发酵短杆菌编码延伸因子P的基因。 谷氨酸棒杆菌 100,000 97-11-01
GB_PL1:SPAC24C9 38666 Z98601 粟酒裂殖酵母I染色体粘粒c24C9。 粟酒裂殖酵母 35,230 99-02-24
(Schizosaccharomyces pombe)
GB_HTG1:CEY102A5 110000 Z99711 Caenorhabditis elegans V染色体克隆Y102A5,***测序正在进行之 Caenorhabditis elegans 37,775 Z99711
_1 中***,为非顺序片段。
表4(续)rxa00152 1419 GB_BA1:MTCY277 38300 Z79701 结核分枝杆菌H37Rv完整基因组;区段65/162。 结核分枝杆菌 58,500 98-06-17
GB_BA1:MSGY456 37316 AD000001 得自克隆y456的结核分枝杆菌序列。 结核分枝杆菌 38,913 1996-12-03
GB_BA2:AF002133 15437 AF002133 鸟分枝杆菌菌株GIR10转录调节物(mav81)基因,部分编码区,顺 鸟分枝杆菌 64,009 1998-03-26
乌头酸酶(acn)、侵染素1(inv1)、侵染素2(inv2)、转录调节物 (Mycobacterium avium)
(moxR)、酮酰基还原酶(fabG)、烯酰醇还原酶(inhA)和亚铁螯合酶
(mav272)基因,完整编码区。rxa00226 948 GB_PR3:AC005756 43299 AC005756 人19号染色体,fosmid 39347,完整序列。 人 36,209 1998-10-12
GB_GSS5:AQ818463 413 AQ818463 HS_5250_A2_B08_SP6E RPCI-11人类男性BAC文库人基因组克隆 人 37,288 99-08-26
平板=826 列=16 行=C,基因组综述序列。
GB_GSS5:AQ782337 832 AQ782337 HS_3184_B1_H12_T7C CIT批准的人类基因组精子文库D人基因 人 35,917 99-08-02
组克隆平板=3184 列=23 行=P,基因组综述序列。rxa00249 980 GB_BA2:AF035608 3614 AF035608 铜绿假单胞菌ATP硫酸化酶小亚基(cysD)和ATP硫酸化酶GTP结 铜绿假单胞菌 50,205 98-06-01
合亚基/APS激酶(cysN)基因,完整编码区。 (Pseudomonas aeruginosa)
GB_BA1:AB017641 17101 AB017641 灰色小单孢菌聚酮化合物合酶的基因,完整编码区。 灰色小单孢菌 40,266 99-04-02
(Micromonospora griseorubida)
GB_BA2:AF002133 15437 AF002133 鸟分枝杆菌菌株GIR10转录调节物(mav81)基因,部分编码区,顺 鸟分枝杆菌 38,429 1998-03-26
乌头酸酶(acn)、侵染素1(inv1)、侵染素2(inv2)、转录调节物
(moxR)、酮酰基还原酶(fabG)、烯酰醇还原酶(inhA)和亚铁螯合酶
(mav272)基因,完整编码区。rxa00299 1101 GB_BA2:CORCSLYS 2821 M89931 谷氨酸棒杆菌βC-S裂合酶(aecD)和支链氨基酸摄入载体(bmQ)基 谷氨酸棒杆菌 100,000 98-06-04
因,完整编码区,和假定的蛋白Yhbw(yhbw)基因,部分编码区。
GB_BA1:CGECTP 2719 AJ001436 谷氨酸棒杆菌ectP基因。 谷氨酸棒杆菌 41,143 98-11-20
GB_BA2:AF181035 5922 AF181035 类球红细菌糖原利用操纵子,完整序列。 类球红细菌 36,701 99-09-07
(Rhodobacter sphaeroides)rxa00332 825 GB_BA1:CGTHRC 3120 X56037 谷氨酸棒杆菌苏氨酸合酶(EC 4.2.99.2)的thrC基因。 谷氨酸棒杆菌 37,730 97-06-17
GB_PAT:109078 3146 109078 得自专利WO8809819的序列4。 未知的 38,700 1994-12-02
GB_PR3:HSJ333B15 73666 AL109954 得自克隆333B15的20号染色体的人类DNA序列,完整序列。 人 37,203 99-11-23rxa00470 1392 GB_PL2:DCPCNAM 865 X62977 胡萝卜增殖细胞核抗原(PCNA)的mRNA。 胡萝卜(Daucus carota) 37,914 99-09-30
表4(续)
GB_PL2:AC006267 101644 AC006267 拟南芥BAC F9M13来自IV染色体的21.5cM附近,完整序列。 拟南芥 36,158 99-04-27
(Arabidopsis thaliana)
GB_BA1:TT10SARNA 721 Y15063 嗜热栖热菌10Sa RNA基因。 嗜热栖热菌(Thermus thermophilus) 39,494 98-08-18rxa00471 813 GB_BA1:SERERYAA 11219 M63676 红色糖多孢菌eryA基因的第一个ORF,完整编码区。 红色糖多孢菌 38,781 93-04-26
(Saccharopolyspora erythraea)
GB_PAT:AR049367 11219 AR049367 得自专利US 5824513的序列1。 未知的 38,781 99-09-29
GB_BA1:SERERYAA 11219 M63676 红色糖多孢菌eryA基因的第一个ORF,完整编码区。 红色糖多孢菌 38,205 93-04-26rxa00499 1404 GB_PR4:AC007206 42732 AC007206 人19号染色体,粘粒R27370,完整序列。 人 34,982 99-04-04
GB_EST26:AI344735 462 AI344735 qp05a10.xl NCI_CGAP_Kid5人cDNA克隆IMAGE:1917114 3’,类 人 42,675 99-02-02
似于gb:M15800 T淋巴细胞成熟相关蛋白(人类);mRNA序列。
GB_PR4:AC006479 161837 AC006479 人克隆DJ1051J04,完整序列。 人 38,462 99-11-11rxa00500 798 GB_PR4:AC006111 190825 AC006111 人16号染色体克隆RPCI-11_461A8,完整序列。 人 40,736 99-07-03
GB_HTG2:AF128834 196589 AF128834 人8号染色体克隆BAC 57G24 map 8p12,***测序正在进行之中 人 34,062 99-02-28
***,为非顺序片段。
GB_HTG2:AF128834 196589 AF128834 人8号染色体克隆BAC 57G24 map 8p12,***测序正在进行之中 人 34,062 99-02-28
***,为非顺序片段。rxa00501 630 GB_BA1:D86429 5925 D86429 直逗号糖多孢菌β半乳糖苷酶的基因,完整编码区。 直逗号糖多孢菌 53,871 1998-12-09
(Saccharopolyspora rectivirgula)
GB_HTG1:HS1099D15 1301 AL035456 人20号染色体克隆RP5-1099D15,***测序正在进行之中***,为非 人 33,546 99-11-23
顺序片段。
GB_HTG1:HS1099D15 1301 AL035456 人20号染色体克隆RP5-1099D15,***测序正在进行之中***,为非 人 33,546 99-11-23
顺序片段。rxa00502 1155 GB_BA2:U00015 42325 U00015 麻风分枝杆菌粘粒B1620。 麻风分枝杆菌 34,783 1994-03-01
(Mycobacterium leprae)
GB_BA1:U00020 36947 U00020 麻风分枝杆菌粘粒B229。 麻风分枝杆菌 34,900 1994-03-01
GB_HTG1:HS179I15 210672 Z84464 人13号染色体克隆179I15,***测序正在进行之中***,为非顺序片 人 32,898 97-01-22
段。rxa00566 729 GB_BA1:MTV008 63033 AL021246 结核分枝杆菌H37Rv完整基因组;区段108/162。 结核分枝杆菌 37,011 98-06-17
GB_BA2:AF071885 2188 AF071885 天蓝色链霉菌ATP依赖性Clp蛋白酶蛋白水解亚基1(clpP1)和 天蓝色链霉菌 62,963 99-06-29
ATP依赖性Clp蛋白酶蛋白水解亚基2(clpP2)基因,完整编码区;
和ATP依赖性Clp蛋白酶ATP结合亚基Clpx(clpX)基因,部分编
码区。
GB_BA2:AF013216 15742 AF013216 黄色粘球菌Dog(dog)、异柠檬酸裂合酶(icl)、Mls(mls)、Ufo(ufo)、 黄色粘球菌(Myxococcus xanthus) 54,683 98-01-28
延胡索酸水合酶(fhy)和蛋白体主要亚基(major subunit)(clpP)基
因,完整编码区;和酰基-CoA氧化酶(aco)基因,部分编码区。
表4(续)rxa00567 714 GB_BA1:MTV008 63033 AL021246 结核分枝杆菌H37Rv完整基因组;区段108/162。 结核分枝杆菌 42,090 98-06-17
GB_BA1:CGBPHI16 962 Y12472 谷氨酸棒杆菌DNA,附着位点噬菌体Phi-16。 谷氨酸棒杆菌 40,000 1999-03-05
GB_BA1:ECOCLPPA 1236 J05534 大肠杆菌ATP依赖性clp蛋白酶蛋白水解组分(clpP)基因,完整编 大肠杆菌 52,119 93-04-26
码区。rxa00621 906 GB_EST1:D36491 360 D36491 CELK033GYFY Yuji Kohara未公布的cDNA Caenorhabditis elegans Caenorhabditis elegans 40,390 94-08-08
cDNA克隆yk33g11 5’,mRNA序列。
GB_IN2:CELC16A3 34968 U41534 Caenorhabditis elegans粘粒C16A3。 Caenorhabditis elegans 35,477 1999-05-18
GB_HTG3:AC009311 160198 AC009311 人克隆NH0311L03,***测序正在进行之中***,3个非顺序片段。 人 38,636 99-08-13rxa00622 1539 GB_BA1:AB004795 3039 AB004795 假单胞菌二肽基氨基肽酶的基因,完整编码区。 假单胞菌(Pseudomonas sp.) 54,721 99-02-05
GB_BA1:MBOPII 2392 D38405 腔隙莫拉氏菌蛋白酶II的基因,完整编码区。 腔隙莫拉氏菌(Moraxella lacunata) 50,167 99-02-08
GB_IN2:AF078916 2960 AF078916 布氏布氏锥虫寡肽酶B(opb)基因,完整编码区。 布氏布氏锥虫 48,076 1999-10-08
(Trypanosoma brucei brucei)rxa00650 759 GB_BA2:AF161327 2021 AF161327 白喉棒杆菌组氨酸激酶ChrS(chrS)和效应调节物ChrA(chrA)基 白喉棒杆菌 51,319 99-09-09
因,完整编码区。
GB_PL2:ATAC006533 99188 AC006533 拟南芥II染色体BAC F20M17基因组序列,完整序列。 拟南芥 38,051 1999-05-26
GB_PL2:ATAC006533 99188 AC006533 拟南芥II染色体BAC F20M17基因组序列,完整序列。 拟南芥 35,403 1999-05-26rxa00675 915 GB_BA1:SC3C8 33095 AL023861 天蓝色链霉菌粘粒3C8。 天蓝色链霉菌 36,836 99-01-15
GB_PR3:AC005736 215441 AC005736 人16号染色体,BAC克隆462G18(LANL)、完整序列。 人 42,027 1998-10-01
GB_IN2:AC005719 188357 AC005719 黑腹果蝇,21号染色体,区域38A5-38B4,BAC克隆BACR48M05, 黑腹果蝇 35,531 1999-10-27
完整序列。 (Drosophila melanogaster)rxa00689 1614 GB_PAT:E07294 2975 E07294 嗜热脂肪芽孢杆菌编码脱氢酶的基因组DNA。 嗜热脂肪芽孢杆菌(Bacillus 45,677 97-09-29
stearotherophilus)
GB_BA1:BACALDHT 1975 D13846 嗜热脂肪芽孢杆菌醛脱氢酶的aldhT基因,完整编码区。 嗜热脂肪芽孢杆菌 45,677 99-02-20
GB_BA2:PPU96338 5276 U96338 恶臭假单胞菌NCIMB 9866质粒pRA4000对甲酚降解途径基因、 恶臭假单胞菌 44,317 1999-05-13
对羟基苯甲醛脱氢酶(pchA)、对甲酚甲基羟化酶、细胞色素亚基前 (Pseudomonas putida)
体(pchC)、未知的(pchX)和对甲酚甲基羟化酶、黄色蛋白亚基(pchF)
基因,完整编码区。rxa00715 918 GB_EST30:AI647104 218 AI647104 vn15c01.y1 Stratagene小鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 58,511 99-04-29
IMAGE:1021248 5’,mRNA序列。
GB_EST17:AA636159 447 AA636159 vn15c01.r1 Stratagene小鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 41,195 1997-10-22
IMAGE:1021248 5’,mRNA序列。
表4(续)
GB_EST10:AA184468 583 AA184468 mt52h05.r1 Stratagene小鼠胚胎癌(#937317)小家鼠cDNA克隆 小家鼠 40,426 97-02-12
IMAGE:633561 5’,类似于gb:D10918小鼠遍在蛋白样蛋白的
mRNA,部分序列(小鼠);mRNA序列。rxa00744 1065 GB_HTG3:AC009855 167592 AC009855 人克隆1_C_5,***测序正在进行之中***,13个非顺序片段。 人 36,673 99-09-03
GB_HTG3:AC009855 167592 AC009855 人克隆1_C_5,***测序正在进行之中***,13个非顺序片段。 人 36,673 99-09-03
GB_PR4:AC005082 169737 AC005082 人克隆RG271G13,完整序列。 人 39,557 99-09-08rxa00756 1119 GB_BA1:MLCB596 38426 AL035472 麻风分枝杆菌粘粒B596。 麻风分枝杆菌 54,562 99-08-27
(Mycobacterium leprae)
GB_GSS12:AQ368028 652 AQ368028 toxb0001N11rCUGI番茄BAC文库番茄基因组克隆 番茄 42,657 99-02-05
toxb0001N11r,基因组综述序列。 (Lycopersicon esculentum)
GB_HTG3:AC008067 151242 AC008067 人克隆NH0303I04,***测序正在进行之中***,2个非顺序片段。 人 37,239 99-09-08rxa00773 1266 GB_BA1:MLU15182 40123 U15182 麻风分枝杆菌粘粒B2266。 麻风分枝杆菌 36,616 1995-03-09
GB_BA1:MSGL611CS 37769 L78822 麻风分枝杆菌粘粒L611 DNA序列。 麻风分枝杆菌 35,714 96-06-15
GB_GSS14:AQ578181 728 AQ578181 nbxb0083P08r CUGI水稻BAC文库稻基因组克隆nbxb0083P08r, 稻(Oryza sativa) 39,246 99-06-02
基因组综述序列rxa00793 1299 GB_GSS5:AQ769737 519 AQ769737 HS_3160_A2_G04_T7C CIT批准的人类基因组精子文库D人基因 人 37,765 99-07-28
组克隆平板=3160 列=8 行=M,基因组综述序列。
GB_BA1:RTU08434 2400 U08434 Rhizobium trifolii乳清酸磷酸核糖基转移酶(pyrE)和果糖激酶(frk)基 Rhizobium trifolii 40,700 97-04-16
因,完整编码区。
GB_EST31:F33810 243 F33810 HSPD27491 HM3人cDNA克隆s3000041E12,mRNA序列。 人 41,564 1999-05-13rxa00820 486 GB_PR4:AC005868 96180 AC005868 人12q24.2PAC RPCI5-944M2(Roswell Park Cancer Institute人类 人 32,298 99-02-27
PAC文库)完整序列。
GB_EST8:AA000903 396 AA000903 mg38b04.r1 Soares小鼠胚胎NbME13.5 14.5小家鼠cDNA克隆 小家鼠 42,045 96-07-18
IMAGE:426031 5’,mRNA序列。
GB_EST25:AI317789 696 AI317789 uj20g09.y1 Sugano小鼠胚胎mewa小家鼠cDNA克隆 小家鼠 38,557 1998-12-17
IMAGE:1920544 5’,类似于WP:C13C4.5 CE08130糖转运蛋白;
mRNA序列。rxa0833 618 GB_PH:BPH6589 41489 AJ006589 噬菌体phi-C31完整基因组。 噬菌体phi-C13 41,806 99-04-29
GB_HTG2:AC006887 215801 AC006887 Caenorhabditis elegans克隆Y59H11,***测序正在进行之中***,3个 Caenorhabditis elegans 35,798 99-02-24
非顺序片段。
GB_HTG2:AC006887 215801 AC006887 Caenorhabditis elegans克隆Y59H11,***测序正在进行之中***,3个 Caenorhabditis elegans 35,798 99-02-24
非顺序片段。rxa00844 957 GB_GSS15:AQ605195 459 AQ605195 HS_2136_B1_C12_T7C CIT批准的人类基因组精子文库D人基因 人 38,074 99-06-10
组克隆平板=2136 列=23 行=F,基因组综述序列。
表4(续)
GB_HTG1:CNS00M8S 214599 AL079302 人14号染色体克隆R-1089B7,***测序正在进行之中***,为顺序 人 38,120 1999-10-15
片段。
GB_HTG1:CNS00M8S 214599 AL079302 人14号染色体克隆R-1089B7,***测序正在进行之中***,为顺序 人 38,120 1999-10-15
片段。rxa00866 1066 GB_BA1:CGORF4GE 2398 X95649 谷氨酸棒杆菌ORF4基因。 谷氨酸棒杆菌 99,273 1998-03-10
N
GB_BA1:BLDAPAB 3572 Z21502 乳发酵短杆菌二氢2,6-吡啶二羧酸合酶和二氢2,6-吡啶二羧酸还 谷氨酸棒杆菌 99,301 93-08-16
原酶的dapA和dapB基因。
GB_PAT:E14517 1411 E14517 编码短杆菌属二氢2,6-吡啶二羧酸还原酶的DNA。 谷氨酸棒杆菌 99,659 99-07-28rxa00877 1788 GB_PAT:I92050 567 I92050 得自专利US 5726299的序列17。 未知的 62,787 1998-12-01
GB_PAT:I78760 567 I78760 得自专利US 5693781的序列16。 未知的 62,787 98-04-03
GB_BA2:AE000426 10240 AE000426 大肠杆菌K-12 MG1655完整基因组的区域316/400。 大肠杆菌 36,456 98-11-12rxa00903 733 GB_BA2:AE001598 11136 AE001598 肺炎衣原体完整基因组的区域14/103。 32,782 1999-03-06
Chlamydiophila pneumoniae
GB_PL2:AF079370 2897 AF079370 乳酸克鲁维酵母转化酶(INV1)基因,完整编码区。 乳酸克鲁维酵母 35,849 99-08-04
(Kluyveromyces lactis)
GB_BA2:AE001598 11136 AE001598 肺炎衣原体完整基因组的区域14/103。 肺炎衣原体 40,138 1999-03-08rxa00905 924 GB_PR2:HSQ15C24 73192 AJ239325 人21号染色体,得自粘粒LLNLc116 1C16和LLNLc116 15C24 map 人 35,076 99-09-28
21q22.3区域D21S171-LA161,完整序列。
GB_GSS4:AQ691923 446 AQ691923 HS_5400_B2_G04_SP6E RPCI-11人类男性BAC文库人基因组克隆 人 33,500 99-07-06
平板=976 列=8 行=N,基因组综述序列。
GB_EST37:AI967802 479 AI967802 Ljimpest 12-930-d6 LjimpλHybriZap双杂种文库Lotus japonicus Lotus japonicus 41,127 99-08-24
cDNA克隆LP930-12-d6 5’,类似于60S核糖体蛋白L7A,mRNA序
列。rxa00906 627 GB_PAT:I78750 588 I78750 得自专利US 5693781的序列6。 未知的 97,071 98-04-03
GB_PAT:I92039 588 I92039 得自专利US 5726299的序列6。 未知的 97,071 1998-12-01
GB_PR3:HS929C8 139190 AL020994 得自克隆929C8的染色体22q12.1-12.3的人类DNA序列。含有 人 39,016 99-11-23
CA重复序列、GSS、STS,完整序列。rxa00907 246 GB_PAT:I78750 588 I78750 得自专利US 5693781的序列6。 未知的 97,561 98-04-03
GB_PAT:192039 588 I92039 得自专利US 5726299的片列6。 未知的 97,561 1998-12-01
GB_PAT:178750 588 178750 得自专利US 5693781的序列6。 未知的 37,222 98-04-03rxa00961 455 GB_BA1:AB032799 9077 AB032799 紫色色杆菌紫色杆素生物合成基因簇(vioA、vioB、vioC、vioD), 紫色色杆菌 39,868 1999-10-02
完整编码区。 (Chromobacterium violaceum)
GB_BA2:AF172851 10094 AF172851 紫色色杆菌紫色杆素生物合成基因簇,完整序列。 紫色色杆菌 42,760 99-08-30
GB_BA1:AB032799 9077 AB032799 紫色色杆菌紫色杆素生物合成基因簇(vioA、vioB、vioC、vioD), 紫色色杆菌 39,551 1999-10-02
完整编码区。
表4(续)rxa00982 1629 GB_BA1:BLARGS 2501 Z21501 乳发酵短杆菌精氨酰-tRNA合成酶和二氨基庚二酸脱羧酶(部分) 谷氨酸棒杆菌 39,003 1993-12-28
的argS和lysA基因。
GB_BA1:CGXLYSA 2344 X54740 谷氨酸棒杆菌精氨酰-tRNA合成酶和二氨基庚二酸脱羧酶(EC 谷氨酸棒杆菌 41,435 93-06-30
4.1.1.20)上游区的argS-lysA操纵子基因。
GB_PAT:E14508 3579 E14508 编码短杆菌属二氨基庚二酸脱羧酶和精氨酰-tRNA合酶的DNA。 谷氨酸棒杆菌 40,566 99-07-28rxa00983 1599 GB_HTG2:AC008152 24000 AC008152 大型利什曼原虫35号染色体克隆L7936 strain Friedlin,***测序正 大型利什曼原虫 38,658 99-07-28
在进行之中***,4个非顺序片段。 (Leishmania major)
GB_HTG2:AC008152 24000 AC008152 大型利什曼原虫35号染色体克隆L7936 strain Friedlin,***测序正 大型利什曼原虫 38,658 99-07-28
在进行之中***,4个非顺序片段。
GB_HTG3:AC008648 87249 AC008648 人5号染色体克隆CIT978SKB_186E14,***测序正在进行之中***, 人 36,102 99-08-03
22个非顺序片段。rxa00984 440 GB_BA1:MVINED 3098 D01045 绿色小单胞菌nedR蛋白和神经氨酸酶的DNA,完整编码区。 绿色小单胞菌 59,226 99-02-02
(Micromonospora viridifaciens)
GB_PAT:E02375 1881 E02375 神经氨酸酶基因。 绿色小单胞菌 59,226 97-09-29
GB_PR4:HUAC00451 101311 AC004513 人16号染色体BAC克隆CIT987SK-A-926E7,完整序列。 人 41,204 99-11-23
3rxa01014 2724 GB_BA1:MTV008 63033 AL021246 结核分枝杆菌H37Rv完整基因组;区段108/162。 结核分枝杆菌 56,167 98-06-17
GB_BA1:STMAMPEP 2849 L23172 浅青紫链霉菌氨肽酶N基因,完整编码区。 浅青紫链霉菌 57,067 1994-05-18
N (Streptomyces lividans)
GB_BA1:SC7H2 42655 AL109732 天蓝色链霉菌粘粒7H2。 天蓝色链霉菌A3(2) 37,551 99-08-02rxa01059 732 GB_HTG3:AC008154 172241 AC008154 人7号染色体,***测序正在进行之中***,26个非顺序片段。 人 39,499 99-09-08
GB_HTG3:AC008154 172241 AC008154 人7号染色体,***测序正在进行之中***,26个非顺序片段。 人 39,499 99-09-08
GB_EST32:AI756574 299 AI756574 ea02f10.y1 Eimeria M5-6裂殖子阶段禽艾美球虫cDNA 5’,mRNA 禽艾美球虫(Eimeria tenella) 37,793 99-06-23
序列。rxa01073 954 GB_BA1:BACOUTB 1004 M15811 枯草杆菌编码孢子形成蛋白的outB基因,完整编码区。 枯草杆菌 53,723 93-04-26
GB_PR4:AC007938 167237 AC007938 来自7q31的人克隆UWGC:djs201,完整序列。 人 34,322 99-07-01
GB_PL2:ATAC006282 92577 AC006282 拟南芥II染色体BAC F13K3基因组序列,完整序列。 拟南芥 36,181 1999-03-13rxa01120 1401 GB_BA1:MTV008 63033 AL021246 结核分枝杆菌HB7Rv完整基因组;区段108/162。 结核分枝杆菌 36,715 98-06-17
GB_BA1:CAJ10321 6710 AJ010321 新月柄杆菌部分tig基因和clpP、cicA、clpX、Ion基因。 新月柄杆菌 63,311 1998-10-01
(Caulobacter crescentus)
表4(续)
GB_BA2:AF150957 4440 AF150957 巴西固氮螺菌触发因子(tig)、热激蛋白ClpP(clpP)和热激蛋白ClpX 巴西固氮螺菌 60,613 99-06-07
(clpX)基因,完整编码区;和Lon蛋白酶(lon)基因,部分编码区。 (Azospirillum brasilense)rxa01147 1383 GB_PR3:HS408N23 97916 Z98048 得自PAC 408N23的染色体22q13上的人类DNA序列。含有HIP、 人 34,567 99-11-23
HSC70-相互作用蛋白(孕酮受体相关P48蛋白)、EST和STS。
GB_BA2:AE001227 26849 AE001227 苍白密螺旋体完整基因组的区域43/87. 苍白密螺旋体 37,564 98-07-16
(Treponema pallidum)
GB_PR3:HS408N23 97916 Z98048 得自PAC 408N23的染色体22q13上的人类DNA序列。含有HIP、 人 34,911 99-11-23
HSC70-相互作用蛋白(孕酮受体相关P48蛋白)、EST和STS。rxa01151 958 GB_BA1:MTCY261 27322 Z97559 结核分枝杆菌H37Rv完整基因组;区段95/162。 结核分枝杆菌 38,789 98-06-17
GB_HTG4:AC009849 114993 AC009849 黑腹果蝇2号染色体克隆BACR07H08(D864)RPCI-98 07.H.8 map 黑腹果蝇 39,213 1999-10-25
31B-31C strain y;cn bw sp,***测序正在进行之中***,55个非顺序
片段。
GB_HTG4:AC009849 114993 AC009849 黑腹果蝇2号染色体克隆BACR07H08(D864)RPCI-98 07.H.8 map 黑腹果蝇 39,213 1999-10-25
31B-31C strain y;cn bw sp,***测序正在进行之中***,55个非顺序
片段。rxa01161 1260 GB_BA2:AF176799 2943 AF176799 戊糖乳杆菌PepQ(pepQ)和分解代谢控制蛋白A(ccpA)基因,完整 戊糖乳杆菌 37,043 99-09-05
编码区。 (Lactobacillus pentosus)
GB_BA2:AF012084 3082 AF012084 瑞士乳杆菌氨酰基脯氨酸二肽酶(pepQ)基因,完整编码区 瑞士乳杆菌 46,796 98-07-01
(Lactobacillus helveticus)
GB_EST32:AI728955 611 AI728955 BNLGHi12114 6天棉花纤维陆地棉cDNA 5’,类似于(AC004481) 陆地棉 37,647 99-06-11
推定的通透酶[拟南芥],mRNA序列。 (Gossypium hirsutum)rxa01181 980 GB_BA1:MLCB22 40281 Z98741 麻风分枝杆菌粘粒B22。 麻风分枝杆菌 61,570 97-08-22
GB_BA1:MTCY190 34150 Z70283 结核分枝杆菌H37Rv完整基因组;区段98/162。 结核分枝杆菌 60,434 98-06-17
GB_BA1:SC5F7 40024 AL096872 天蓝色链霉菌粘粒5F7。 天蓝色链霉菌A3(2) 57,011 99-07-22rxa01182 516 GB_HTG1:CEY116A8 2110000 Z98858 Caenorhabditis elegans IV染色体克隆Y116A8,***测序正在进行之 Caenorhabditis elegans 34,843 99-10-26
- 中***,为非顺序片段。
GB_HTG1:CEY116A8 2110000 Z98858 Caenorhabditis elegans IV染色体克隆Y116A8,***测序正在进行之 Caenorhabditis elegans 34,843 99-10-26
- 中***,为非顺序片段。
GB_IN1:CEY116A8C 260341 AL117204 Caenorhabditis elegans粘粒Y116A8C,完整序列。 Caenorhabditis elegans 34,843 99-11-19rxa01189 732 GB_BA1:D90915 130001 D90915 Synechocystis sp、PCC6803完整基因组,17/27,2137259-2267259。 Synechocystis sp. 36,538 99-02-07
GB_BA1:D90915 130001 D90915 Synechocystis sp、PCC6803完整基因组,17/27,2137259-2267259。 Synechocystis sp. 34,512 99-02-07
表4(续)
GB_HTG3:AC010515 41038 AC010515 人19号染色体克隆LLNL-R_249H9,***测序正在进行之中***,31 人 33,564 99-09-15
个非顺序片段。rxa01192 681 GB_OM:CFP180RRC 5425 X87224 家犬核糖体受体的mRNA,p180 家犬(Canis familiaris) 41,229 99-01-22
GB_OM:CFP180RRC 5425 X87224 家犬核糖体受体的mRNA,p180 家犬 38,187 99-01-22rxa01214 1614 GB_IN1:CEY47D3A 199814 AL117202 Caenorhabditis elegans粘粒Y47D3A,完整序列。 Caenorhabditis elegans 36,604 99-011-19
GB_PR4:AC006039 176257 AC006039 人克隆NH0319F03,完整序列。 人 34,984 1999-05-05
GB_PR4:AC006039 176257 AC006039 人克隆NH0319F03,完整序列。 人 35,951 1999-05-05rxa01224 1146 GB_EST22:AI070047 479 AI070047 UI-R-C1-In-f-08-0-UI.s1 UI-R-C1褐鼠cDNA克隆UI-R-C1-In-f-08-0- 褐鼠(Rattus norvegicus) 36,975 99-07-05
UI 3’,mRNA序列。
GB_RO:S75965 625 S75965 THP=Tamm-Horsfall蛋白{启动子}[大鼠,基因组,625 nt]。 鼠(Rattus sp.) 34,400 95-07-27
GB_EST5:H96951 459 H96951 yu01g03.r1 Soares_pineal_gland_N3HPG人cDNA克隆 人 32,969 1995-12-11
IMAGE:232564 5’,mRNA序列。rxa01250 588 GB_PL1:NEULCCB 2656 M18334 粗糙脉孢霉(菌株TS)漆酶基因,完整编码区。 粗糙脉孢霉(Neurospora crassa) 44,330 1994-05-03
GB_OV:MTRACOMP 16714 Y16884 Rhea americana完整线粒体基因组。 Mitochondroin Rhea americana 35,094 99-07-19
L
GB_OV:AF090339 16704 AF090339 Rhea americana完整线粒体基因组。 Mitochondroin Rhea americana 35,094 1999-05-27rxa01277 2127 GB_PL2:AF111709 52684 AF111709 Oryza sativa subsp.indica Retrosat I反转录转座子和Ty3-Gypsy型 Oryza sativa subsp.indica 37,410 99-04-26
Retrosat 2反转录转座子,完整序列;和未知基因。
GB_IN1:CELZC250 34372 AF003383 Caenorhabditis elegans粘粒ZC250。 Caenorhabditis elegans 35,506 1997-05-14
GB_EST1:Z14808 331 Z14808 CEL5E4 Chris Martin分类的cDNA文库Caenorhabditis elegans Caenorhabditis elegans 36,890 97-06-19
cDNA克隆cm5e4 5’,mNRA序列。rxa01302 576 GB_BA1:MTCI65 34331 Z95584 结核分枝杆菌H37Rv完整基因组;区段50/162。 结核分枝杆菌 59,298 98-06-17
GB_BA1:MSGY348 40056 AD000020 得自克隆y348的结核分枝杆菌序列。 结核分枝杆菌 59,227 1996-12-10
GB_BA1:SC5C7 41906 AL031515 天蓝色链霉菌粘粒5C7。 天蓝色链霉菌 39,261 98-09-07rxa01303 1458 GB_BA1:TTAJ5043 837 AJ225043 嗜热栖热菌部分narK基因。 嗜热栖热菌 55,245 98-06-18
GB_PL2:AC010675 84723 AC010675 拟南芥I染色体BAC T17F3基因组序列,完整序列。 拟南莽 37,058 99-11-11
GB_GSS9:AQ170862 518 AQ170862 HS_3165_B2_F03_T7 CIT批准的人类基因组精子文库D人基因组 人 38,610 1998-10-17
克隆平板=3165 列=6 行=L,基因组综述序列。rxa01308 2503 GB_BA1:D90757 17621 D90757 大肠杆菌基因组DNA。(27.3-27.7分钟)。 大肠杆菌 55,445 99-02-07
GB_BA1:D90787 15942 D90787 大肠杆菌基因组DNA,Kohara克隆#276(33.0-33.3分钟)。 大肠杆菌 36,815 1997-05-29
GB_BA1:D90758 13860 D90758 大肠杆菌基因组DNA。(27.6-27.9分钟)。 大肠杆菌 54,942 99-02-07rxa01309 824 GB_BA1:SCJ12 35302 AL109989 天蓝色链霉菌粘粒J12。 天蓝色链霉菌A3(2) 62,423 99-08-24
GB_BA1:BSNARYWI 12450 Z49884 枯草杆菌nar[G,H,I,J,K]、ywi[C,D,E]和argS基因。 枯草杆菌 57,447 98-06-24
表4(续)
GB_BA1:BSUB0020 212150 Z99123 枯草杆菌完整基因组(区域20/21):3798401-4010550。 枯草杆菌 37,129 97-11-26rxa01358 1644 GB_GSS11:AQ260413 453 AQ260413 CITBI-E1-2510B12.TF CITBI-E1人基因组克隆2510B12,基因组综 人 41,531 1998-10-24
述序列。
GB_EST20:AA840582 326 AA840582 vw77h07.r1 Stratagene小鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 42,901 98-02-27
IMAGE:1261021 5’,类似于gb:J04181小鼠A-X肌动蛋白mRNA,
完整编码区(小鼠);mRNA序列。
GB_PAT:A39944 3836 A39944 得自专利WO9421807的序列1。 未鉴定的 38,764 1997-03-05rxa01385 2004 GB_BA1:FVBPENTA 2519 M98557 黄杆菌五氯苯酚4-单加氧酶基因,完整的mRNA。 黄杆菌(Flavobacterium sp.) 40,855 93-04-26
GB_PAT:I19994 2516 119994 得自专利US 5512478的序列2。 未知的 40,855 1996-10-07
GB_BA2:AF059680 2410 AF059680 鞘氨醇单胞菌UG30五氯苯酚4-单加氧酶(pcpB)基因,完整编码 鞘氨醇单胞菌(Sphingomonas sp.) 42,993 99-04-27
区;和五氯苯酚4-单加氧酶还原酶(pcpD)基因,部分编码区。 UG30rxa01412 327 GB_GSS12:AQ332469 459 AQ332469 HS_5003_A1_H08_SP6E RPCI11人类男性BAC文库人基因组克隆 人 38,208 1999-03-06
平板=579 列=15 行=O,基因组综述序列。
GB_EST27:AA998532 453 AA998532 UI-R-C0-ic-d-11-0-UI.s1 UI-R-C0褐鼠cDNA克隆UI-R-C0-ic-d-11- 褐鼠 39,336 1999-03-09
0-UI 3’,mRNA序列。
GB_HTG1:HSA342D1 178183 AL121748 人10号染色体克隆RP11-342D11,***测序正在进行之中***,为非 人 40,550 99-11-23
1 顺序片段。rxa01458 1173 GB_BA2:AE000745 15085 AE000745 Aquifex aeolicus完整基因组的区域77/109。 Aquifex aeolicus 37,694 1998-03-25
GB_BA2:AE000745 15085 AE000745 Aquifex aeolicus完整基因组的区域77/109。 Aquifex aeolicus 35,567 1998-03-25rxa01571 723 GB_BA1:AB011413 12070 AB011413 灰色链霉菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因,部分和 灰色链霉菌(Streptomyces griseus) 57,500 98-08-07
完整的编码区。
GB_BA1:AB011413 12070 AB011413 灰色链霉菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因,部分和 灰色链霉菌 35,655 98-08-07
完整的编码区。rxa01607 753 GB_PR4:AC005005 133893 AC005005 得自22的人PAC克隆DJ412A9,完整序列。 人 38,399 1999-03-02
GB_HTG3:AC008257 109187 AC008257 黑腹果蝇2号染色体克隆BACR08A11(D916)RPCI-98 08.A.11 map 黑腹果蝇 33,741 1999-10-08
42A-42A strain y;cn bw sp,***测序正在进行之中***,93个非顺序
片段。
GB_HTG3:AC008257 109187 AC008257 黑腹果蝇2号染色体克隆BACR08A11(D916)RPCI-98 08.A.11 map 黑腹果蝇 33,741 1999-10-08
42A-42A strain y;cn bw sp,***测序正在进行之中***,93个非顺序
片段。rxa01609 996 GB_BA1:MTV003 13246 AL008883 结核分枝杆菌H37Rv完整基因组;区段125/162。 结核分枝杆菌 39,369 98-06-17
GB_BA1:MSGB1529C 36985 L78824 麻风分枝杆菌粘粒B1529 DNA序列。 麻风分枝杆菌 60,624 96-06-15
S
GB_BA1:AB024601 14807 AB024601 铜绿假单胞菌四氢2,6-吡啶二羧酸N-琥珀酰基转移酶的dapD基 铜绿假单胞菌 41,603 1999-03-12
因,完整编码区,菌株PAO1。 (Pseudomonas aeruginosa)
表4(续)rxa01654 1119 GB_GSS4:AQ704352 532 AQ704352 HS_2147_A2_H04_MR CIT批准的人类基因组精子文库D人基因 人 37,838 99-07-07
组克隆平板=2147 列=8 行=O,基因组综述序列。
GB_RO:MMAE000663 250611 AE000663 小家鼠TCRβ基因座,所述完整序列的碱基1-250611(区域1/3)。 小家鼠 35,799 97-09-04
GB_EST23:AI158428 511 AI158428 ud24f12.f1 Soares 2NbMT小家鼠cDNA克隆IMAGE:1446863 5’, 小家鼠 41,337 98-09-30
mRNA序列。rxa01664 945 GB_OV:AF026198 63155 AF026198 Fugu rubripes神经细胞粘着分子L1同系物(L1-CAM)基因,完整编 Fugu rubripes 35,187 1998-05-02
码区;推定的蛋白1(PUT1)基因,部分编码区;有丝分裂特异性
染色体分离蛋白SMC1同系物(SMC1)基因,部分编码区;和钙通
道α-1亚基同系物(CCA1)和推定的蛋白2(PUT2)基因,部分编码
区,完整序列。
GB_PR3:AC004466 122186 AC004466 人12q13.1 PAC RPCI5-1057I20(Roswell Park Cancer Institute人类 人 37,382 98-09-17
PAC文库)完整序列。
GB_PR3:AC004466 122186 AC004466 人12q13.1 PAC RPCI5-1057I20(Roswell Park Cancer Institute人类 人 37,325 98-09-17
PAC文库)完整序列。rxa01795 720 GB_BA2:CGU13922 4412 U13922 谷氨酸棒杆菌推定的II型5-胞嘧啶甲基转移酶(cgIIM)和推定的II 谷氨酸棒杆菌 99,444 98-02-03
型限制性内切核酸酶(cgIIR)和推定的I型或III型限制性内切核酸
酶(clgIIR)基因,完整编码区。
GB_BA1:S86113 1044 S86113 ORF 1[淋病奈瑟氏球菌,基因组,1044nt]。 淋病奈瑟氏球菌 58,320 1993-05-07
(Neisseria gonorrhoeae)
GB_PAT:I22080 850 I22080 得自专利US 5525717的序列1。 未知的 57,722 1996-10-07rxa01802 954 GB_BA2:AE001519 14062 AE001519 幽门螺杆菌菌株J99完整基因组的区域80/132。 幽门螺杆菌(Helicobacter pylori) 33,510 99-01-20
J99
GB_GSS5:AQ774071 552 AQ774071 HS_2269_B1_C10_T7C CIT批准的人类基因组精子文库D人基因 人 37,967 99-07-29
组克隆平板=2269 列=19 行=F,基因组综述序列。
GB_PR4:AC007459 40907 AC007459 人16号染色体克隆306C6,完整序列。 人 39,140 1999-05-04rxa01838 842 GB_BA1:SCE15 26440 AL049707 天蓝色链霉菌粘粒E15。 天蓝色链霉菌 36,297 99-04-22
GB_HTG3:AC009545 165042 AC009545 人11号染色体克隆131_J_04 map 11,***测序正在进行之中***,8 人 37,651 1999-10-01
个非顺序片段。
GB_HTG3:AC009545 165042 AC009545 人11号染色体克隆131_J_04 map 11,***测序正在进行之中***,8 人 37,651 1999-10-01
个非顺序片段。rxa01848 867 GB_BA1:MTCY24A1 20270 Z95207 结核分枝杆菌H37Rv完整基因组;区段124/162。 结核分枝杆菌 38,270 98-06-17
GB_EST21:C89252 587 C89252 C89252小鼠早期胚泡cDNA小家鼠cDNA克隆01B00061JC08, 小家鼠 37,219 1998-05-28
mRNA序列。
GB_EST14:AA423340 457 AA423340 ve39d04.r1 Soares小鼠乳腺NbMMG小家鼠cDNA克隆 小家鼠 38,377 1997-10-16
IMAGE:820519 5’,mRNA序列。
表4(续)rxa01849 1224 GB_BA1:MTCY24A1 20270 Z95207 结核分枝杆菌H37Rv完整基因组;区段124/162。 结核分枝杆菌 39,950 98-06-17
GB_BA2:RCPHSYNG 45959 Z11165 夹膜红细菌的完整光合作用基因簇。 夹膜红细菌 37,344 99-09-02
(Rhodobacter capsulatus)
GB_BA1:RSP010302 40707 AJ010302 类球红细菌光合作用基因簇。 类球红细菌 40,898 99-08-22rxa01868 2049 GB_BA1:MTV033 21620 AL021928 结核分枝杆菌H37Rv完整基因组;区段11/162。 结核分枝杆菌 38,679 98-06-17
GB_BA1:MLCL622 42498 Z95398 麻风分枝杆菌粘粒L622。 麻风分枝杆菌 38,911 97-06-24
GB_BA1:MSGB983CS 36788 L78828 麻风分枝杆菌粘粒B983 DNA序列。 麻风分枝杆菌 38,933 96-06-15rxa01885 924 GB_BA1:MTCY1A10 25949 Z95387 结核分枝杆菌H37Rv完整基因组;区段117/162。 结核分枝杆菌 51,094 98-06-17
GB_PR3:HSU220B11 41247 Z69908 得自粘粒cU220B11的X染色体上标记DXS6791和DXS8038之间 人 39,038 99-11-23
的人类DNA序列。
GB_BA1:PDU17435 993 U17435 脱氮副球菌Fnr样转录激活蛋白(nnr)基因,完整编码区。 脱氮副球菌 39,390 95-07-19
(Paracoccus denitrificans)rxa01914 526 GB_PR3:AC005796 43843 AC005796 人19号染色体,粘粒R31408,完整序列。 人 34,961 1998-10-06
GB_PR3:HS390C10 114231 AL008721 得白BAC 390C10的染色体22q11.21-12.1上的人DNA序列。含有 人 39,600 99-11-23
免疫球蛋白样基因和类似于β晶体蛋白的假基因。含有EST、STS、
GSS和taga以及tat重复多态性,完整序列。
GB_PR3:AC005796 43843 AC005796 人19号染色体,粘粒R31408,完整序列。 人 37,725 1998-10-06rxa01932 1020 GB_PR3:AC003025 112309 AC003025 人类染色体11p12.2PAC克隆pDJ466a11,完整序列。 人 35,585 98-07-23
GB_GSS3:B78728 312 B78728 CIT-HSP-431E3.TV CIT-HSP人基因组克隆431E3,基因组综述序 人 38,907 98-06-25
列。
GB_PR3:AC003025 112309 AC003025 人类染色体11p12.2PAC克隆pDJ466a11,完整序列。 人 35,859 98-07-23rxa01933 726 GB_HTG1:HS74O16 169401 AL110119 人21号染色体克隆RPCIP704O1674 map 21q21,***测序正在进行 人 35,302 99-08-27
之中***,为非顺序片段。
GB_HTG1:HS74O16 169401 AL110119 人21号染色体克隆RPCIP704O1674 map 21q21,***测序正在进行 人 35,302 99-08-27
之中***,为非顺序片段。
GB_PR4:AC006032 170282 AC006032 得自Y的人BAC克隆NH0115E20,完整序列。 人 37,640 99-02-27rxa01971 954 GB_HTG3:AC008230 108469 AC008230 黑腹果蝇2号染色体克隆BACR17I17(D934)RPCI-98 17.I.17 map 黑腹果蝇 35,466 99-08-10
53A-53C strain y;cn bw sp,***测序正在进行之中***,108个非顺序
片段。
GB_HTG3:AC008230 108469 AC008230 黑腹果蝇2号染色体克隆BACR17I17(D934)RPCI-98 17.I.17 map 黑腹果蝇 35,466 99-08-10
53A-53C strain y;cn bw sp,***测序正在进行之中***,108个非顺序
片段。
表4(续)
GB_PR3:AF064860 165382 AF064860 人染色体21q22.3PAC 70I24,完整序列。 人 39,716 98-06-02rxa02016 900 GB_EST2:D48846 459 D48846 RICS15292A水稻绿苗稻cDNA,mRNA序列。 稻 37,118 95-08-02
GB_GSS10:AQ195886 595 AQ195886 RPCI11-66O13.TJ RPCI-11人基因组克隆RPCI-11-66O13,基因组 人 41,000 99-04-20
综述序列。
GB_GSS10:AQ195886 595 AQ195886 RPCI11-66O13.TJ RPCI-11人基因组克隆RPCI-11-66O13,基因组 人 34,790 99-04-20
综述序列。rxa02017 807 GB_EST20:AA855266 406 AA855266 vw70b08.r1 Stratagene小鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 42,638 1998-03-06
IMAGE:1260279 5’,mRNA序列。
GB_EST20:AA855266 406 AA855266 vw70b08.r1 Stratagene小鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 37,183 1998-03-06
IMAGE:1260279 5’,mRNA序列。rxa02018 1073 GB_BA1:SC5C7 41906 AL031515 天蓝色链霉菌粘粒5C7。 天蓝色链霉菌 41,732 98-09-07
GB_BA1:MTC165 34331 Z95584 结核分枝杆菌H37Rv完整基因组;区段50/162。 结核分枝杆菌 62,395 98-06-17
GB_BA1:SCJ12 35302 AL109989 天蓝色链霉菌粘粒J12。 天蓝色链霉菌A3(2) 61,603 99-08-24rxa02048 1497 GB_PAT:E15823 2323 E15823 得自产氨棒杆菌的编码细胞表面蛋白的DNA。 产氨棒杆菌 53,942 99-07-28
(Corynebacterium ammoniagenes)
GB_OM:SSAMPTDN 3387 Z29522 S.scrofa氨肽酶N的mRNA。 Sus scrofa 42,672 94-09-26
GB_OV:D87992 3181 D87992 原鸡氨肽酶Ey的mRNA,完整编码区。 原鸡(Gallus gallus) 41,554 99-06-05rxa02101 1386 GB_BA1:AP000064 247695 AP000064 Aeropyrum pernix基因组DNA,区域7/7。 Aeropyrum pernix 39,882 99-06-22
GB_PL2:ATAC006587 79262 AC006587 拟南芥II染色体BAC T17D12基因组序列,完整序列。 拟南芥 38,490 1999-03-23
GB_PL2:ATAC006587 79262 AC006587 拟南芥II染色体BAC T17D12基因组序列,完整序列。 拟南芥 34,863 1999-03-23rxa02265 423 GB_BA2:AF120718 4137 AF120718 发酵乳杆菌脲酶操纵子,部分序列。 发酵乳杆菌 56,265 1999-03-31
(Lactobacillus fermentum)
GB_PAT:E03531 2896 E03531 编码酸性脲酶的DNA序列。 发酵乳杆菌 56,265 97-09-29
GB_BA1:LBAAURE 2896 D10605 发酵乳杆菌酸性脲酶的基因。 发酵乳杆菌 56,265 99-02-02rxa02276 801 GB_GSS10:AQ242920 451 AQ242920 HS_2061_A1_B08_MR CIT批准的人类基因组精子之库D人基因 人 37,916 1998-10-03
组克隆平板=2061 列=15 行=I,基因组综述序列。
GB_IN1:SLMMTPMF 14503 D29637 多头绒泡菌线粒体DNA。 多头绒泡菌线粒体(Mitochondrion 40,335 1999-05-12
Physarum Polycephalum)
GB_IN2:AF012249 5542 AF012249 得自mF质粒的多头绒泡菌菌株线粒体aux2-S区,包括URFA’、 多头绒泡菌线粒体 40,335 1998-05-08
URFC、URFD、URFE、URFF和URFG基因,完整编码区,和URFH
基因,部分编码区。rxa02277 738 GB_BA2:AF048784 681 AF048784 内氏放线菌脲酶辅助蛋白(ureG)基因,完整编码区。 内氏放线菌 66,814 99-02-09
(Actinomyces naeslundii)
表4(续)
GB_BA2:AF056321 5482 AF056321 内氏放线菌脲酶γ亚基UreA(ureA)、脲酶β亚基UreB(ureB)、脲酶α 内氏放线菌 63,686 99-02-09
亚基UreC(ureC)、脲酶辅助蛋白UreE(ureE)、脲酶辅助蛋白UreF
(ureF)、脲酶辅助蛋白UreG(ureG)和脲酶辅助蛋白UreD(ureD)基
因,完整编码区。
GB_BA2:SSU35248 5773 U35248 唾液链球菌ure簇镍转运蛋白同系物(ureI)基因,部分编码区,和脲 唾液链球菌 61,931 96-01-26
酶β亚基(ureA)、γ亚基(ureB)、α亚基(ureC)和辅助蛋白(ureE)、 (Streptococcus salivarius)
(ureF)、(ureG)和(ureD)基因,完整编码区。rxa02278 972 GB_GSS3:B49054 543 B49054 RPCI11-4I13.TV RPCI-11人基因组克隆RPCI-11-4I13,基因组综述 人 39,161 99-04-08
序列。
GB_PL1:PMCMSGI 3363 L27092 卡氏肺囊虫B细胞受体(msgI)基因,3’端。 卡氏肺囊虫 39,819 94-09-26
(Pneumocystis carinii)
GB_PL2:AF038556 12792 AF038556 卡氏肺囊虫人致病变种主要表面糖蛋白(msg1,msg3,msg4)基因的 卡氏肺囊虫人致病变种 33,832 98-09-10
变异区,部分编码区。 (Pneumocystis carinii f.sp.hominis)rxa02317 735 GB_GSS8:AQ051031 914 AQ051031 nbxb0004dG10r CUGI水稻BAC文库稻基因组克隆nbxb0004N20r, 稻 32,299 1999-03-24
基因组综述序列。
GB_GSS8:AQ051031 914 AQ051031 nbxb0004dG10r CUGI水稻BAC文库稻基因组克隆nbxb0004N20r, 稻 34,573 1999-03-24
基因组综述序列。rxa02334 746 GB_BA1:CGU35023 3195 U35023 谷氨酸棒杆菌硫代硫酸转硫酶(thtR)基因,部分编码区,酰基CoA 谷氨酸棒杆菌 100,000 97-01-16
羧化酶(accBC)基因,完整编码区。
GB_BA1:MTCY71 42729 Z92771 结核分枝杆菌H37Rv完整基因组;区段141/162。 结核分枝杆菌 60,380 99-02-10
GB_BA1:U00012 33312 U00012 麻风分枝杆菌粘粒B1308。 麻风分枝杆菌 37,660 96-01-30rxa02351 1039 GB_HTG2:HS225E12 126464 AL031772 人6号染色体克隆RP1-225E12 map q24,***测序正在进行之中***, 人 35,973 1999-12-03
为非顺序片段。
GB_HTG2:HS225E12 126464 AL031772 人6号染色体克隆RP1-225E12 map q24,***测序正在进行之中***, 人 35,973 1999-12-03
为非顺序片段。
GB_HTG2:HS225E12 126464 AL031772 人6号染色体克隆RP1-225E12 map q24,***测序正在进行之中***, 人 36,992 1999-12-03
为非顺序片段。rxa02410 789 GB_BA1:AB020624 1605 AB020624 谷氨酸棒杆菌D-谷氨酸消旋酶的murl基因,完整编码区。 谷氨酸棒杆菌 99,227 99-07-24
GB_EST4:H51527 294 H51527 yo33b09.s1 Soares成人脑N2b4HB55Y人cDNA克隆IMAGE:179705 人 40,411 95-09-18
3’,mRNA序列。
GB_GSS1:CNS003CM 1101 AL064136 黑腹果蝇(果蝇)的RPCI-98文库的BAC#BACR08C19的黑腹果蝇 黑腹果蝇 37,674 99-06-03
基因组综述序列T7末端,基因组综述序列。
表4(续)rxa02477 744 GB_HTG4:AC010054 130191 AC010054 黑腹果蝇染色体3L/74E2克隆RPCI98-15E10,***测序正在进行之 黑腹果蝇 37,466 1999-10-16
中***,70个非顺序片段。
GB_HTG4:AC010054 130191 AC010054 黑腹果蝇染色体3L/74E2克隆RPCI98-15E10,***测序正在进行之 黑腹果蝇 37,466 1999-10-16
中***,70个非顺序片段。
GB_HTG4:AC009375 137069 AC009375 黑腹果蝇染色体3L/75AI克隆RPCI98-44L18,***测序正在进行之 黑腹果蝇 39,18 1999-10-16
中***,59个非顺序片段。rxa02513 832 GB_BA1:MTER260 373 X92572 土分枝杆菌32kDa蛋白的基因(部分)。 土分枝杆菌(Mycobacterium terrae) 42,895 98-01-15
GB_PL1:AB019229 84294 AB019229 拟南芥基因组DNA,3号染色体,P1克隆:MDC16,完整序列, 拟南芥 36,084 99-11-20
GB_PL1:AB019229 84294 AB019229 拟南芥基因组DNA,3号染色体,P1克隆:MDC16,完整序列, 拟南芥 35,244 99-11-20rxa02531 834 GB_BA1:CGLATTB 271 X89850 谷氨酸棒杆菌attB区的DNA。 谷氨酸棒杆菌 40,590 96-08-08
GB_EST11:AA239557 423 AA239557 mv25f04.r1 GuayWoodford Beier小鼠肾第0天小家鼠cDNA克隆 小家鼠 38,760 1997-03-12
IMAGE:656095 5’,类似于gb:X52634鼠类tlm蛋白的tlm癌基因(小
鼠);mRNA序列。
GB_BA1:RSPYPPCL 6500 AL002398 类球红细菌pyp和pcl基因和orfA、orfB、orfC、orfD、orfE、orfF。 类球红细菌 37,091 1998-12-17rxa02548 314 GB_BA2:AF127374 63734 AF127374 淡紫灰链霉菌LinA同系物、细胞色素P450羟化酶ORF4、细胞色 淡紫灰链霉菌 66,242 1999-05-27
素P450羟化酶ORF3、MitT(mitT)、MitS(mitS)、MitR(mitR)、mitQ (Streptomyces lavendulae)
(mitQ)、MitP(mitP)、MitO(mitO)、MitN(mitN)、MitM(mitM)、
MitL(mitL)、MitK(mitK)、MitJ(mitJ)、MitI(mitI)、MitH(mitH)、
MitG(mitG)、MitF(mitF)、MitE(mitE)、MitD(mitD)、MitC(mitC)、
MitB(mitB)、MitA(mitA)、MmcA(mmcA)、MmcB(mmcB)、MmcC
(mmcC)、MmcD(mmcD)、MmcE(mmcE)、MmcF(mmcF)、MmcG
(mmcG)、MmcH(mmcH)、MmcI(mmcI)、MmcJ(mmcJ)、MmcK
(mmcK)、MmcL(mmcL)、MmcM(mmcM)、MmcN(mmcN)、MmcO
(mmcO)、Mrd(mrd)、MmcP(mmcP)、MmcQ(mmcQ)、MmcR
(mmcR)、MmcS(mmcS)、MmcT(mmcT)、MmcU(mmcU)、MmcV
(mmcV)、Mct(mct)、Mmcw(mmcW)、MmcX(mmcX)和MmcY
(mmcY)基因,完整编码区;和未知基因。
表4(续)
GB_BA2:AF127374 63734 AF127374 淡紫灰链霉菌LinA同系物、细胞色素P450羟化酶ORF4、细胞色 淡紫灰链霉菌 38,411 1999-05-27
素P450羟化酶ORF3、MitT(mitT)、MitS(mitS)、MitR(mitR)、mitQ
(mitQ)、MitP(mitP)、MitO(mitO)、MitN(mitN)、MitM(mitM)、
MitL(mitL)、MitK(mitK)、MitJ(mitJ)、MitI(mitI)、MitH(mitH)、
MitG(mitG)、MitF(mitF)、MitE(mitE)、MitD(mitD)、MitC(mitC)、
MitB(mitB)、MitA(mitA)、MmcA(mmcA)、MmcB(mmcB)、MmcC
(mmcC)、MmcD(mmcD)、MmcE(mmcE)、MmcF(mmcF)、MmcG
(mmcG)、MmcH(mmcH)、MmcI(mmcI)、MmcJ(mmcJ)、MmcK
(mmcK)、MmcL(mmcL)、MmcM(mmcM)、MmcN(mmcN)、MmcO
(mmcO)、Mrd(mrd)、MmcP(mmcP)、MmcQ(mmcQ)、MmcR
(mmcR)、MmcS(mmcS)、MmcT(mmcT)、MmcU(mmcU)、MmcV
(mmcV)、Mct(mct)、MmcW(mmcW)、MmcX(mmcX)和MmcY
(mmcY)基因,完整编码区;和未知基因。
GB_GSS4:AQ741886 742 AQ741886 HS_5569_B2_B02_SP6 RPCI-11人类男性BAC文库人基因组克隆 人 38,907 9907-16
平板=1145 列=4 行=D,基因组综述序列。rxa02558 1098 GB_EST18:AA567307 741 AA567307 HL01004.5prime HL黑腹果蝇头BlueScript黑腹果蝇cDNA克隆 黑腹果蝇 38,736 98-11-28
HL01004 5prime,mRNA序列。
GB_EST27:AI402394 630 AI402394 GH21610.5prime GH黑腹果蝇头pOT2黑腹果蝇cDNA克隆 黑腹果蝇 41,308 99-02-08
GH21610 5prime,mRNA序列。
GB_GSS10:AQ237646 715 AQ237646 RPCI11-61I9.TJB RPCI-11人基因组克隆RPCI-11-61I9,基因组综 人 44,340 99 04-21
述序列。rxa02565 1389 GB_EST32:AI726448 562 AI726448 BNLGHi5854 6天棉花纤维陆地棉cDNA 5’,类似于(U53418)UDP- 陆地棉 37,003 99-06-11
葡萄糖脱氢酶[大豆],mRNA序列。
GB_EST32:AI726198 608 AI726198 BNLGHi5243 6天棉花纤维陆地棉cDNA 5’,类似于(U53418)UDP- 陆地棉 40,925 99-06-11
葡萄糖脱氢酶[大豆],mRNA序列。
GB_PR4:AC002992 154848 AC002992 人Y染色体,克隆203M13,完整序列。 人 38,039 1999-10-13rxa02574 1311 GB_EST4:H29653 415 H29653 ym58f01.r1 Soares婴儿脑1NIB人cDNA克隆IMAGE:52678 5’,类 人 39,036 95-07-17
似于SP:OXDD_BOVIN P31228 D-天冬氨酸氧化酶;mRNA序列。
GB_PR3:HSDJ261K5 131974 AL050350 得自克隆261K5的染色体6q21-22.1的人类DNA序列。含有新型 人 35,957 99-11-23
有机阳离子转运蛋白基因(BAC ORF RG331P03)的3’部分、D-天冬
氨酸氧化酶(EC 1.4.3.1)的DDO基因、EST、STS、GSS和两个推定
的CpG岛,完整序列。
表4(续)
GB_EST2:R20147 494 R20147 yg18h02.r1 Soares婴儿脑1NIB人cDNA克隆IMAGE:32866 5’,类 人 36,437 95-04-17
似于SP:OXDD_BOVIN P31228 D-天冬氨酸氧化酶;mRNA序列。rxa02589 888 GB_HTG1:CEY6E2 186306 Z96799 Caenorhabditis elegans V染色体克隆Y6E2,***测序正在进行之中 Caenorhabditis elegans 37,979 1997-10-02
***,为非顺序片段。
GB_HTG1:CEY6E2 186306 Z96799 Caenorhabditis elegans V染色体克隆Y6E2,***测序正在进行之中 Caenorhabditis elegans 37,979 1997-10-02
***, 为非顺序片段。
GB_HTG3:AC011690 72277 AC011690 人克隆17_E_13,低通序列采样(LOW-PASS SEQUENCE 人 35,814 1999-10-10
SAMPLING)。rxa02592 894 GB_BA1:MSGB983CS 36788 L78828 麻风分枝杆菌粘粒B983DNA序列。 麻风分枝杆菌 53,235 96-06-15
GB_GSS9:AQ170723 487 AQ170723 HS_2270_B2_F05_MR CIT批准的人类基因组精子文库D人基因组 人 39,666 1998-10-16
克隆平板=2270 列=10 行=L,基因组综述序列。
GB_GSS12:AQ349397 791 AQ349397 RPCI11-118H16.TJ RPCI-11人基因组克隆RPCI-11-118H16,基因 人 34,204 1999-05-07
组综述序列。rxa02603 1119 GB_BA1:MTV026 23740 AL022076 结核分枝杆菌H37Rv完整基因组;区段157/162。 结核分枝杆菌 37,975 99-06-24
GB_IN2:AC005714 177740 AC005714 黑腹果蝇,2R染色体,区域58D4-58E2,BAC克隆BACR48M13, 黑腹果蝇 41,226 1999-05-01
完整序列。
GB_EST19:AA775050 218 AA775050 ac76e10.s1 Stratagene肺(#937210)人cDNA克隆IMAGE:868554 3’, 人 40,826 98-02-05
类似于gb:Y00371_mal热激同源物(cognate)71KD蛋白(人类);
mRNA序列。rxa02630 1446 GB_BA1:MLCL373 37304 AL035500 麻风分枝杆菌粘粒L373。 麻风分枝杆菌 49,015 99-08-27
GB_BA1:MTV044 16150 AL021999 结核分枝杆菌H37Rv完整基因组;区段45/162。 结核分枝杆菌 49,192 98-06-17
GB_BA1:MLU15180 38675 U15180 麻风分枝杆菌粘粒B1756。 麻风分枝杆菌 45,621 1995-03-09rxa02643 1167 GB_EST37:AI950576 308 AI950576 wx52e08.x1 NCI_CGAP_Lu28人cDNA克隆IMAGE:2547302 3’, 人 40,909 99-09-06
mRNA序列。
GB_EST37:AI950576 308 AI950576 wx52e08.x1 NCI_CGAP_Lu28人cDNA克隆IMAGE:2547302 3’, 人 40,288 99-09-06
mRNA序列。rxa02644 774 GB_EST34:AV149547 302 AV149547 AV149547小家鼠C57BL/6J 10-11天胚胎小家鼠cDNA克隆 小家鼠 38,627 99-07-05
2810489D03,mRNA序列。
GB_EST35:AV156221 271 AV156221 AV156221小家鼠头C57BL/6J 12天胚胎小家鼠cDNA克隆 小家鼠 33,990 99-07-07
3000001C24,mRNA序列。
GB_EST32:AV054919 274 AV054919 AV054919小家鼠胰C57BL/6J成年小家鼠cDNA克隆 小家鼠 36,585 99-06-23
1810033C08,mRNA序列。rxa02745 902 GB_BA1:MTV007 32806 AL021184 结核分枝杆菌H37Rv完整基因组;区段64/162。 结核分枝杆菌 39,298 98-06-17
表4(续)
GB_BA2:AF027770 30683 AF027770 耻垢分枝杆菌FxbA(fxbA)基因,部分编码区;FxbB(fxbB)、FxbC 耻垢分枝杆菌 55,125 1998-12-03
(fxbC)和FxuD(fxtD)基因,完整编码区和未知的基因。 (Mycobacterium smegmatis)
GB_BA2:SAU43537 3938 U43537 Streptomyces argillaceus光神霉素抗性决定簇、ATP结合蛋白(mtrA) Streptomyces argillaceus 46,868 96-09-05
和膜蛋白(mtrB)基因,完整编码区。rxa02746 290 GB_BA1:CAJ10319 5368 AJ010319 谷氨酸棒杆菌amtP、glnB、glnD基因和部分ftsY和srp基因。 谷氨酸棒杆菌 100,000 1999-05-14
GB_BA1:MTCY338 29372 Z74697 结核分枝杆菌H37Rv完整基因组;区段127/162。 结核分枝杆菌 39,785 98-06-17
GB_HTG3:AC008733 216140 AC008733 人19号染色体克隆CITB-E1_2525J15,***测序正在进行之中***, 人 35,688 99-08-03
72个非顺序片段。rxa02820 1411 GB_BA1:BFU64514 3837 U64514 坚强芽孢杆菌dppABC操纵子、二肽转运蛋白dppA基因,部分编 坚强芽孢杆菌 36,859 97-02-01
码区,和二肽转运蛋白dppB和dppC基因,完整编码区。 (Bacillus firmus)
GB_IN1:CET04C10 20958 Z69885 Caenorhabditis elegans粘粒T04C210。完整序列。 Caenorhabditis elegans 35,934 99-09-02
GB_EST35:AI823090 720 AI823090 L30-944T3 Ice植物λUni-Zap XR表达文库,30小时NaCl处理 Mesembryanthemum crystallinum 35,770 99-07-21
Mesembryanthemum crystallinum cDNA克隆L30-944 5’,类似于60S
核糖体蛋白L36(AC004684)[拟南芥],mRNA序列。rxa02834 518 GB_BA1:CJY13333 3315 Y13333 空肠弯曲杆菌clpB基因 空肠弯曲杆菌 53,400 99-04-12
(Campylobacter jejuni)
GB_BA2:AF065404 181654 AF065404 炭疽芽孢杆菌毒性质粒PX01,完整序列。 炭疽芽孢杆菌(Bacillus anthracis) 45,168 1999-10-20
GB_PL2:AC006601 110684 AC006601 拟南芥V染色体60.5cM附近的图谱,完整序列。 拟南芥 36,680 99-02-22实施例部分实施例1:谷氨酸棒杆菌ATCC13032的总基因组DNA的制备
让谷氨酸棒杆菌(ATCC 13032)的培养物于30℃下在BHI培养基(Difco)中剧烈振荡生长过夜。通过离心收集细胞,弃去上清液,将细胞重悬于5ml缓冲液I(培养物原始体积的5%--所有指定的体积均已经根据100ml培养物体积来计算)。缓冲液I的组成:140.34g/l蔗糖、2.46g/l
×7
、10ml/l
溶液(100g/l,用
调至pH6.7)、50ml/l M12浓缩物(10g/l
、1g/l
、2g/l
×7
、0.2g/l
、0.5g/l酵母膏(Difco)、10ml/l微量元素混合物(200mg/l
×
、10mg/l
×7
、3mg/l
×4
、30mg/l
、20mg/l
×6
、1mg/l
×6
、3mg/l ×2
、500mg/l络合剂(EDTA或柠檬酸)、100ml/l维生素混合物(0.2mg/l生物素、0.2mg/l叶酸、20mg/对氨基苯甲酸、20mg/l核黄素、40mg/l泛酸钙(ca-panthothenate)、140mg/烟酸、40mg/l盐酸吡哆醇、200mg/l肌醇)。将溶菌酶加入悬浮液中至终浓度2.5mg/ml。于37℃温育约4小时后,细胞壁被降解,通过离心收获所产生的原生质体。沉淀用5ml缓冲液I洗涤1次,用5ml TE缓冲液(10mM
、1mMEDTA,pH 8)洗涤1次。将沉淀重悬于4ml TE缓冲液中,加入0.5mlSDS溶液(10%)和0.5ml
溶液(5M)。加入蛋白酶K至终浓度200μg/ml后,将悬浮液于37℃温育约18小时。采用标准方法,通过用苯酚、苯酚-氯仿-异戊醇和氯仿-异戊醇抽提,纯化DNA。然后,通过加入1/50体积的3M乙酸钠和2体积乙醇,然后于-20℃温育30分钟,在高速离心机中用SS34转子(Sorvall)以12,000rpm离心30分钟,沉淀所述DNA。将DNA溶于1ml含有20μg/ml RNA酶A的TE缓冲液中,于4℃对1000ml TE缓冲液透析至少3小时。在此期间,更换缓冲液3次。向0.4ml等份的经透析的DNA溶液中,加入0.4ml 2M
和0.8ml乙醇。于-20℃温育30分钟后,通过离心(13,000rpm,BiofugeFresco,Heraeus,Hanau,德国)收集DNA。将DNA沉淀溶于TE缓冲液中。用该方法制备的DNA可以用于所有目的,包括DNA印迹法或基因组文库的构建。实施例2:构建谷氨酸棒杆菌ATCC13032在大肠杆菌中的基因组文库
采用实施例1所述制备的DNA按照已知的明确方法(参见例如Sambrook,J.等,(1989)“Molecular Cloning:A Laboratory Manual”,ColdSpring Harbor Laboratory Press,或Ausubel,F.M.等(1994)“CurrentProtocols in Molecular Biology”,John Wiley & Sons.)构建粘粒和质粒文库。
可以使用任何质粒或粘粒。具体使用的质粒是质粒pBR322(Sutcliffe,J.G.(1979)Proc.Natl.Acad.Sci.USA,75:3737-3741)、pACYC177(Change & Cohen(1978)J.Bacteriol 134:1141-1156)、pBS质粒系列(pBSSK+、pBSSK-和其它系列;Stratagene,LaJolla,USA)或粘粒如SuperCosl(Stratagene,LaJolla,USA)或Lorist6(Gibson,T.J.,Rosenthal A.和Waterson,R.H.(1987)Gene 53:283-286)。使用质粒pSL109可构建特别用于谷氨酸棒杆菌的基因文库(Lee,H-S.和A.J.Sinskey(1994)J.Microbiol.Biotechnol.4:256-263)。实施例3:DNA测序和计算功能分析
应用实施例2描述的基因组文库按照标准方法、尤其是利用ABI377测序仪的链终止法(参见例如Fleischman,R.D.等(1995)“流感嗜血杆菌Rd.的随机全基因组测序和装配”,Science,269:496-512)进行DNA测序。使用具有以下核苷酸序列的测序引物:5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。实施例4:体内诱变
将质粒(或其它载体)DNA通过保持其遗传信息完整性的能力受损的大肠杆菌或其它微生物(例如芽孢杆菌或酵母如酿酒酵母)进行传代,从而可在体内诱变谷氨酸棒杆菌。典型增变株的DNA修复系统基因存在突变(例如mutHLS、mutD、mutT等;参考文献见Rupp,W.D.(1996)DNA修复机制,载于:Escherichia coli and Salmonella,第2277-2294页,ASM:Washington.)。这类菌株是本领域技术人员周知的。例如Greener,A.和Callahan,M.(1994)Strategies 7:32-34中介绍了所述菌株的使用。实施例5:大肠杆菌和谷氨酸棒杆菌之间的DNA转移
若干棒杆菌属和短杆菌属菌种含有自主复制(综述参见例如Martin,J.F.等(1987)Biotechnology,5:137-146)的内源性质粒(例如pHM1519或pBL1)。采用其中加入复制起点和谷氨酸棒杆菌合适标记的大肠杆菌标准载体(Sambrook,J.等(1989),“Molecular Cloning:ALaboratory Manual”,Cold Spring Harbor Laboratory Press或Ausubel,F.M.等(1994)“Current Protocols in Molecular Biology”,John Wiley & Sons)能够容易地构建大肠杆菌和谷氨酸棒杆菌的穿梭载体。所述复制起点优选取自分离自棒杆菌属和短杆菌属菌种的内源性质粒。具体使用的这些菌种的转化标记为卡那霉素抗性基因(例如Tn5或Tn903转座子产生的卡那霉素抗性基因)或氯霉素抗性基因(Winnacker,E.L.(1987),From Genes to Clones-Introduction to Gene Technology,VCH,Weinheim)。所述文献中列举了许多关于构建各种穿梭载体的实施例,所述穿梭载体可在大肠杆菌和谷氨酸棒杆菌中复制而且可用于若干目的,包括基因过量表达(参考文献参见例如Yoshihama,M.等(1985)J.Bacteriol.162:591-597,Martin J.F.等(1987)Biotechnology,5:137-146和Eikmanns,B.J.等(1991)Gene,102:93-98)。
应用标准方法可将目的基因克隆到一种上述穿梭载体并将这种杂合载体导入谷氨酸棒杆菌菌株中。可采用以下方法转化谷氨酸棒杆菌:原生质体转化(Kastsumata,R.等(1984)J.Bacteriol.159306-311)、电穿孔(Liebl,E.等(1989)FEMS Microbiol.Letters,53:399-303),如果使用特殊载体,也可以采用接合方法(见例如Sch_fer,A等(1990)J.Bacteriol.172:1663-1666)。通过制备谷氨酸棒杆菌质粒DNA(利用本领域周知的标准方法)并将其转化入大肠杆菌,也可以使穿梭载体从谷氨酸棒杆菌转移到大肠杆菌。采用标准方法可进行这种转化步骤,但是最好使用Mcr缺陷型大肠杆菌菌株如NM522(Gough和Murray(1983)J.Mol.Biol.166:1-19)。
可使用包含pCG1(美国专利号4,617,267)或其片段和任选包含TN903卡那霉素抗性基因(Grindley,N.D.和Joyce,C.M.(1980)Proc.Natl.Acad.Sci.USA 77(12):7176-7180)的质粒在谷氨酸棒杆菌菌株中过量表达各种基因。此外,也可以使用质粒pSL109在谷氨酸棒杆菌菌株中过量表达各种基因(Lee,H.-S.和A.J.Sinskey(1994)J.Microbiol.Biotechnol.4:256-263)。
除了使用复制型质粒之外,也可以通过整合入基因组实现基因过量表达。利用周知的方法可实现在谷氨酸棒杆菌或其它棒杆菌属或短杆菌属菌种中的基因组整合,例如与基因组区的同源重组、限制性内切核酸酶介导的整合(REMI)(参见例如DE专利19823834)或应用转座子。也可以采取以下措施修饰调节区(例如启动子、阻抑蛋白和/或增强子)而调节目的基因的活性:利用定点方法(例如同源重组)或基于随机事件的方法(例如转座子诱变或REMI)进行序列修饰、插入或缺失。也可以在本发明一个或多个基因编码区的3’插入转录终止子作用的核酸序列;所述终止子是本领域周知的,例如在Winnacker,E.L.(1987)FromGenes to Clones-Introduction to Gene Technology.VCH:Weinheim)中有其介绍。实施例6:评价突变蛋白的表达
观测转化宿主细胞中的突变蛋白活性依赖于这样的事实:突变蛋白以与野生型蛋白相似的方式和相似的量表达。一种确定突变基因转录水平(有效翻译出基因产物的mRNA量的指标)的有用方法是进行RNA印迹(参考文献见例如Ausubel等(1988)Current Protocols inMolecular Biology,Wiley:纽约),其中用可检测标记(通常为放射性或化学发光)标记结合目的基因的设计引物,使得当提取所述生物培养物的总RNA、在凝胶上电泳、转移至稳定基体上以及与所述探针温育时,所述探针的结合以及结合量可说明所述基因的存在而且指示该基因的mRNA量。这样的信息是突变基因转录程度的证据。应用若干本领域周知的方法可从谷氨酸棒杆菌制备总细胞RNA,例如Bormann,E.R.等(1992)Mol.Microbiol.6:317-326介绍的方法。
为了评价所述mRNA翻译蛋白的存在或相对量,可使用标准技术例如蛋白质印迹(参见例如Ausubel等(1988)Current Protocols inMolecular Biology,Wiley:纽约)。在此过程中,提取总细胞蛋白,经凝胶电泳分离,转移至如硝酸纤维素的基体上以及与特异性结合所需蛋白的探针如抗体温育。这种探针一般用容易检测的化学发光或比色标记物标记。观测到标记物存在和标记物量说明所述细胞中存在所需突变蛋白及其量。实施例7:遗传改良的谷氨酸棒杆菌的培养-培养基和培养条件
用合成或天然生长培养基培养遗传改良棒杆菌。用于棒杆菌的许多不同生长培养基是众所周知的而且容易获得(Lieb等(1989)Appl.Microbiol.Biotechnol.,32:205-210;von der Osten等(1998)Biotechnology Letters,11:11-16;专利DE 4,120,867;Liebl(1992)“棒杆菌属”,载于:The Procaryotes,第II卷,Balows,A.等编著,Springer-Verlag)。这些培养基的组成是:一种或多种碳源、氮源、无机盐、维生素和微量元素。优选碳源为糖如单糖、二糖或多糖。例如葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉或纤维素为非常好的碳源。也可以通过复合化合物如糖蜜或精制糖的其它副产品为培养基提供糖。提供不同碳源的混合物也可能是有益的。其它可能的碳源为醇类和有机酸,例如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物,或者含有这些化合物的物质。典型氮源包括氨气或铵盐,例如氯化铵或硫酸铵、氢氧化铵、硝酸盐、尿素、氨基酸或复合氮源如玉米浆、大豆粉、大豆蛋白、酵母膏、肉膏等。
培养基可含有的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的盐酸盐、亚磷酸盐或硫酸盐。所述培养基中可加入螯合化合物,以保持溶液中的金属离子。特别有效的螯合化合物包括二羟基酚(例如儿茶酚或原儿茶酸)或有机酸(例如柠檬酸)。所述培养基还常规含有其它生长因子,例如维生素或生长促进剂,其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和吡哆醇。生长因子和盐通常源自复合培养基组分,例如酵母膏、糖蜜、玉米浆等。培养基化合物的确切组成与直接实验密切相关而且具体取决于各特定情况。关于培养基优化的信息可在教科书“Applied Microbiol.Physiology,A PracticalApproach(P.M.Rhodes,P.F.Stanbury编著,IRL Press(1997),第53-73页,ISBN 0 19 963577 3)中获得。也可以从商业供应商选择生长培养基,例如标准1号(Merck)或BHI(grain heart infusion,DIFCO)等。
所有培养基组分通过加热灭菌(于1.5帕和121℃ 20分钟)或过滤除菌。所述组分可一起灭菌,如果需要可分别灭菌。所有培养基组分可培养开始时就存在,或者可以任选连续或分批加入。
每个实验分别规定培养条件。温度应为15℃-45℃。培养温度可保持恒定或实验中改变。培养基的pH应为5-8.5、优选约7.0,可在培养基中加入缓冲剂维持pH。用于该目的的典型缓冲剂为磷酸钾缓冲剂。可选择或同时使用合成缓冲剂如MOPS、HEPES、ACES等。也可以在培养期间加入氢氧化钠或氢氧化铵维持恒定培养pH。如果使用复合培养基组分如酵母膏,额外缓冲剂需要可能降低,因为实际上许多复合化合物具有高缓冲能力。如果使用发酵罐培养所述微生物,也可以使用氨气控制pH。
培养时间通常为数小时至数天。选择培养时间以使液体培养基累积的产物量最多。可用各种容器进行所公开的生长实验,例如微量滴定板、玻璃试管、玻璃烧瓶或不同大小的玻璃或金属发酵罐。为了筛选大量克隆,培养微生物应使用微量滴定板、玻璃试管或摇瓶(带有或没有挡板)。优选使用100ml摇瓶,其中装有10%(体积)需要的生长培养基。培养瓶应在使用100-300rpm速度范围的旋转式振摇器(振幅为25mm)上振摇。维持湿润气氛使蒸发丧失最少;或者应进行数学校正蒸发损失。
如果测试经遗传修饰的克隆,也应该测试未经修饰的对照克隆或含没有任何插入片段的基础质粒的对照克隆。利用已经于30℃温育的琼脂平板如CM平板(10g/l葡萄糖、2.5g/l氯化钠、2g/l尿素、10g/l聚胨、5g/l酵母膏、5g/l肉膏、22g/l氯化钠、2g/l尿素、10g/l聚胨、5g/l酵母膏、5g/l肉膏、22g/l琼脂、pH 6.8,2M氢氧化钠)上生长的细胞接种培养基至OD600为0.5-1.5。加入得自CM平板的谷氨酸棒杆菌细胞的盐水悬浮液或加入该菌的液体预培养物,从而完成培养基接种。实施例8:体外分析突变蛋白的功能
本领域已有明确的酶活性和动力学参数测定法。必须使测定任何给定改变酶活性的实验适合野生型酶的比活,这完全在本领域一般技术人员能力范围内。关于酶的一般概述以及涉及结构、动力学、原理、方法、应用的具体细节和许多酶活性测定实例可见于例如以下文献:Dixon,M.和Webb,E.C.,(1979)Enzymes,Longmans:London;Fersht,(1985)Enzyme Structure and Mechanism,Freeman:纽约;Walsh,(1979)Enzymatic Reaction Mechanisms.Freeman:San Francisco;Price,N.C.,Stevens,L.(1982)Fundamentals of Enzymology.Oxford Univ.Press:Oxford;Boyer,P.D.编著(1983)The Enzymes,第三版,Academic Press:纽约;Bisswanger,H.,(1994)Enzymkinetik,第二版,VCH:Weinheim(ISBN 3527300325);Bergmeyer,H.U.,Bergmeyer,J.,Graβ1,M.编著(1983-1986)Methods of Enzymatic Analysis,第三版,第I-XII卷,VerlagChemie:Weinheim;以及Ullmann’s Encyclopedia of Industrial Chemistry(1987),第A9卷,“Enzymes”,VCH:Weinheim,第352-363页。
可应用数种明确的方法如DNA条带移位测定(也称为凝胶阻滞测定)测定结合DNA的蛋白活性。可用报道基因测定(例如Kolmar,H.等(1995)EMBO J.14:3895-3904以及其中的引用文献介绍的测定)测量所述蛋白对其它分子表达的影响。报道基因测试系统是众所周知的而且明确用于原核生物细胞和真核生物细胞,利用酶如β半乳糖苷酶、绿色荧光蛋白等。
按照例如Gennis,R.B.(1989)“膜孔、通道与转运蛋白”,载于:Biomembranes,Molecular Structure and Function,Springer:Heidelberg,第85-137、199-234和270-322页介绍的技术可测定膜转运蛋白的活性。实施例9:分析突变蛋白对所需产物产量的影响
可如下评价谷氨酸棒杆菌的遗传改进对所需化合物(例如氨基酸)产量的影响:使经修饰的微生物在合适条件(例如上述条件)下生长,然后分析所述培养基和/或细胞组分中的所需产物(即氨基酸)的产量增加情况。所述分析技术是本领域一般技术人员周知的,包括光谱检测法、薄层层析、各种染色法、酶法以及微生物学方法、以及分析型层析法如高效液相层析(参见例如Ullman,Encyclopedia of IndustrialChemistry,第A2卷,第89-90和443-613页,VCH:Weinheim(1985);Fallon,A.等,(1987)“HPLC在生物化学中的应用”,载于:LaboratoryTechniques in Biochemistry and Molecular Biology,第17卷;Rehm等(1993)Biotechnology,第3卷,第III章:“产物的回收纯化”,第469-714页,VCH:Weinheim;Belter,P.A.等(1988)Bioseparations:downstream processing for biotechnology,John Wiley和Sons;Kennedy,J.F.和Cabral,J.M.S.(1992)Recovery processes for biological materials,John Wiley和Sons;Shaeiwitz,J.A.和Henry,J.D.(1988)生物化学分离纯化(Biochemical separations),载于:Ulmann’s Encyclopedia of IndustrialChemistry,第B3卷,第11章,第1-27页,VCH:Weinheim;以及Dechow,F.J.(1989)Separation and purification techniques inbiotechnology,Noyes Publications)。
除了检测发酵终产物之外,也可以分析利用产生所需产物的代谢途径的其它组分,例如中间体和副产物,以确定产生所述化合物的总体效率。分析方法包括测量培养基中的营养物水平(例如糖、烃类、氮源、磷酸盐和其它离子)、检测生物量组成和生长、分析生物合成途径共同代谢物的产量以及发酵期间产生的气体。这些检测的标准方法概述于Applied Microbial Physiology,A Practical Approach,P.M.Rhodes和P.F.Stanbury编著,IRL Press,第103-129、131-163和165-192页(ISBN:0199635773)以及其中引用的参考文献。实施例10:从谷氨酸棒杆菌培养物纯化所需产物
可用本领域周知的各种方法从上述培养物的谷氨酸棒杆菌细胞或上清液中回收所需产物。如果所需产物不从细胞中分泌出来,可以通过低速离心从培养物收获细胞,应用标准技术裂解细胞,例如机械力或超声。离心去除细胞碎片,保留含有所述可溶性蛋白的上清液部分供进一步纯化所需要的化合物。如果产物从谷氨酸棒杆菌细胞分泌出来,则通过低速离心从所述培养物中去除细胞,保留上清液部分供进一步纯化。
使得自任一纯化方法的上清液部分在合适树脂上进行层析,其中所需分子保留在层析树脂上而样品中的大多数杂质被洗出,或者杂质保留在树脂上而所述样品被洗出。根据需要可重复所述层析步骤,使用相同或不同层析树脂。本领域一般技术人员在选择合适层析树脂和最有效利用待纯化的特定分子方面是非常精通的。纯化产物可通过过滤或超滤浓缩,贮藏在产物最稳定的温度状态下。
有许多本领域已知的纯化方法,上述纯化方法仅仅是举例说明本发明。这样的纯化技术描述于例如Bailey,J.E.& Ollis,D.F.,BiochemicalEngineering Fundamentals,McGraw-Hill:纽约(1986)。
应用本领域的标准技术可评价分离化合物的身份和纯度。所述标准技术包括高效液相层析(HPLC)、光谱法、染色法、薄层层析、NIRS、酶测定法或微生物学测定法。所述分析方法的综述见:Patek等(1994)Appl.Environ.Microbiol.60:133-140;Malakhova等(1996)Biotekhnologiya 11:27-32;以及Schmidt等(1998)BioprocessEngineer,19:67-70;Ulmann’s Encyclopedia of Industrial Chemistry,(1996)第A27卷,VCH:Weinheim,第89-90、521-540、540-547、559-566、575-581和581-587页;Michal,G.(1999)Biochemical Pathways:An Atlas of Biochemistry and Molecular Biology,John Wiley and Sons;Fallon,A.等(1987)HPLC在生物化学中的应用,载于:LaboratoryTechniques in Biochemistry and Molecular Biology,第17卷。实施例11:分析本发明的基因序列
比较序列并确定两种序列的同源性百分率是本领域已知的技术,可以采用数学算法完成,例如Karlin和Altschul的算法((1990)Proc.Natl.Acad.Sci.USA 87:2264-68)及其改良算法(Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-77。这种算法掺入了Altschul等(1990)J.Mol.Biol.215:403-10的NBLAST和XBLAST程序(2.0版)。可应用NBLAST程序,分值=100,字节长度=12进行BLAST核苷酸检索,以便获得与本发明HA核酸分子同源的核苷酸序列。可应用XBLAST程序,分值=50,字节长度=3进行BLAST蛋白质检索,以便获得与本发明HA蛋白分子同源的氨基酸序列。为了获得比较目的的空位对比序列,可按照Altschnl等,(1997)Nucleic Acids Res.25(17):3389-3402所述,使用引入空位的(Gapped)BLAST。当使用BLAST和引入空位的BLAST程序时,本领域技术人员将知道如何优化用于分析特定序列的程序(例如XBLAST和NBLAST)参数。
用于序列比较的另一个数学算法实例是Meyers和Miller算法((1988)Comput.Appl.Biosci.4:11-17)。这种算法掺入了ALIGN程序(2.0版),ALIGN程序是GCG序列比对软件包的组成部分。当使用ALIGN程序比较氨基酸序列时,可使用PAM120加权残基表、空位长度罚分12和空位罚分4。其它序列分析算法是本领域已知的,包括Torelli和Robotti(1994)Comput.Appl.Biosci.10:3-5介绍的ADVANCE和ADAM;以及Pearson和Lipman(1988)P.N.A.S.85:2444-8介绍的FASTA。
也可以使用GCG软件包中的GAP程序(可在http://www.gcg.com获得),选用Blosum 62矩阵或PAM250矩阵以及空位加权为12、10、8、6或4而长度加权为2、3或4,获得两种氨基酸序列的同源性百分率。应用GCG软件包中的GAP程序,使用标准参数,例如空位加权为50而长度加权为3,可获得两种核酸序列的同源性百分率。
采用本领域已知的技术(参见例如Bexevanis和Ouellette编著(1998)Bioinformatics:A Practical Guide to the Analysis of Genes and Proteins,John Wiley和Sons:纽约),可进行本发明基因序列与Genbank中的序列的比较分析。以3步骤方法比较本发明基因序列与Genbank中的基因。第一步,对本发明各序列与Genbank核苷酸序列进行BLASTN分析(例如局部比对分析),保留前500个命中序列供进一步分析。接下来,对500个命中序列进行FASTA检索(例如联合的局部和全局序列比对分析,其中比对序列的限定区)。最后,采用GCG软件包中的GAP程序(使用标准参数),对本发明各基因序列与各前3个FASTA命中序列进行全局性序列比对。为了获得正确的结果,应用本领域周知的方法使从Genbank取出的序列长度调整为查询序列长度。分析结果见表4。获得的数据与单独对本发明各基因与Genbank各参考序列进行GAP(全局)分析获得的数据相同,但是与这样的数据库-宽GAP(全局)分析相比,需要显著减少计算时间。没有获得高于阈值的序列比对的本发明序列在表4中指示为没有序列比对信息。本领域技术人员进而应该知道的是,表4表头“同源性(%)(GAP)”下的GAP序列比对同源性百分率是以欧洲数字格式列出的,其中“,”代表小数点。例如该栏中的数值“40,345”是指“40.345%”。实施例12:构建和操作DNA微阵列
此外,本发明序列可以用于构建和应用DNA微阵列(DNA阵列的设计、方法学以及使用是本领域众所周知的,例如在以下文献中有其介绍:Schena,M等(1995)Science 270:467-470;Wodicka,L.等(1997)Nature Biotechnology 15:1359-1367;DeSaizieu,A.等(1998)NatureBiotechnology 16:45-48;以及DeRisi,J.L.等(1997)Science 278:680-686)。
DNA微阵列是固体或柔性支持物,例如硝酸纤维素、尼龙、玻璃、硅酮或其它物质构成的材料。核酸分子与支持物表面有序结合。适当标记后,其它核酸或核酸混合物可与固定化核酸分子杂交,标记物可用来监测和测量限定区域中的杂交分子的各信号强度。该方法可同时定量所施用的核酸样品或混合物中全部或选定核酸的相对量或绝对量。因此,DNA微阵列可平行分析多种(多达6800种或更多)核酸的表达(参见例如Schena,M.(1996)BioEssays 18(5):427-431)。
本发明序列可用来设计寡核苷酸引物,该寡核苷酸引物能够通过诸如聚合酶链式反应的核酸扩增反应扩增一种或多种谷氨酸棒杆菌基因的规定区域。选择和设计5’或3’寡核苷酸引物或合适接头,可使所产生的PCR产物与上述支持物介质表面共价连接(也参见例如Schena,M.等(1995)Science 270:467-470介绍)。
按照Wodicka,L.等(1997)Nature Biotechnology 15:1359-1367介绍,也可以通过原位寡核苷酸合成构建核酸微阵列。应用光刻法使基体精确限定的区域曝光。由此活化光不稳定性保护基团而加入核苷酸,而避光区则不会发生任何改变。随后的保护和光活化循环使得在限定位置合成不同的寡核苷酸。通过固相合成寡核苷酸,可在微阵列上合成所限定区域的本发明基因。
样品或核苷酸混合物中存在的本发明核酸分子可与所述微阵列杂交。按照标准方法可标记这些核酸分子。简而言之,例如逆转录或DNA合成时通过掺入同位素或荧光标记核苷酸而标记核酸分子(例如mRNA分子或DNA分子)。已有关于标记核酸与微阵列杂交的介绍(例如载于:Schena,M等(1995)同上;Wodicka,L等(1997),同上;以及DeSaizieu A.等(1998),同上)。杂交分子的检测和定量须适合具体掺入的标记物。例如按照Schena,M等(1995)(同上)介绍可检测放射性标记物,而例如按照Shalon等((1996)Genome Research 6:639-645)的方法可检测荧光标记物。
将本发明序列用于上述DNA微阵列技术可比较分析谷氨酸棒杆菌或其它棒杆菌属的不同菌株。核酸阵列方法学有助于例如根据各个转录物分布研究菌株间变异和鉴定特定和/或所需菌株特性如致病性、生产能力和胁迫耐受性的重要基因。此外,利用核酸阵列技术可以比较发酵反应期间本发明基因的表达分布。实施例13:分析细胞蛋白群动力学(蛋白质组学(Proteomics))
本发明基因、组合物和方法可用于研究蛋白群相互作用和动力学,称为“蛋白质组学”。目的蛋白群包括但不限于谷氨酸棒杆菌总蛋白群(例如与其它生物的蛋白群比较)、在特定环境或代谢条件下(例如发酵期间的高温或低温,或者高pH或低pH)具有活性的蛋白、在生长和发育的特定时期具有活性的蛋白。
可用本领域周知的各种技术如凝胶电泳分析蛋白质群。例如通过裂解或提取可获得细胞蛋白,应用各种电泳技术可使细胞蛋白彼此分开。十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)主要根据蛋白质的分子量分离蛋白。等电聚焦聚丙烯酰胺凝胶电泳(IEF-PAGE)通过其等电点(它不仅反映氨基酸序列,而且反映所述蛋白翻译后修饰)分离蛋白。另一个更优选的蛋白分析方法是连续组合的IEF-PAGE和SDS-PAGE,称为2-D-gel电泳(例如见以下文献介绍:Hermann等(1998)Electrophoresis 19:3217-3221;Fountoulakis等(1998)Electrophoresis 19:1193-1202;Langen等(1997)Electrophoresis 18:1184-1192;Antelmann等(1997)Electrophoresis 18:1451-1463)。也可以使用其它蛋白分离技术进行蛋白质分离,例如毛细管凝胶电泳;所述技术是本领域周知的技术。
可用标准技术如染色或标记显现所述方法分开的蛋白。合适染色法是本领域已知的,包括考马斯亮蓝、银染色或荧光染料如Sypro Ruby(Molecular Probes)。在谷氨酸棒杆菌培养基中含有放射性标记氨基酸或其它蛋白前体(例如35S-甲硫氨酸、35S-半胱氨酸、14C-标记氨基酸、15N-氨基酸、
或
或13C-标记氨基酸)可在其分离前标记这些细胞的蛋白。同样可使用荧光标记物。可按照前述技术提取、分开和分离所述标记蛋白。
通过测量所用染料或标记物量可进一步分析所述技术显现的蛋白。采用例如光学方法可定量测定给定蛋白量,而且可以与相同凝胶或其它凝胶上的其它蛋白的量比较。例如采用光学比较、光谱法、图象扫描和凝胶分析、或者通过应用照相胶片和筛选可进行凝胶上的蛋白比较。这样的技术产本领域周知的。
为了确定任何给定蛋白的身份,可采用直接测序或其它标准技术。可使用例如N-和/或C-末端氨基酸测序(例如Edman降解法),也可以使用质谱法(尤其是MALDI或ESI技术(参见例如Langen等(1997)Electrophoresis 18:1184-1192))。本文提供的蛋白序列可用于通过所述技术鉴定谷氨酸棒杆菌蛋白。
所述方法获得的信息可用于比较各种生物条件(例如不同生物、发酵时间点、培养基条件或不同群落生境等)的不同样品之间的蛋白存在、活性或修饰的模式。这些实验单独或与其它技术联合获得的资料可用于各种用途,例如比较给定(例如代谢)情况的各种生物行为、提高生产精细化学品的菌株生产能力或提高生产精细化学品的产率。等同实施方案
本领域技术人员知道或者仅采用常规实验就能够确定本文介绍的本发明的具体实施方案的许多等同实施方案。以下的权利要求书包括这样的等同实施方案。
序列表<110>BASF公司(BASF Aktiengesellschaft)<120>编码参与体内稳态和适应的蛋白质的谷氨酸棒杆菌基因<130>BGI-128CPPC<140>PCT/IB00/00911<141>2000-06-23<160>440<210>1<211>314<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(291)<223>RXA02548<400>1cca ccg atc tac ttc tcc cac gac cgc gaa gtt ttc gag cgc gac ggc 48Pro Pro Ile Tyr Phe Ser His Asp Arg Glu Val Phe Glu Arg Asp Gly1 5 10 15atg tgg ctg acc gca ggc gag tgg ggt gga cca aag aag ggc gag gag 96Met Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Lys Lys Gly Glu Glu
20 25 30atc gtc acc aag act gtc cgc tac cgc acc gtc ggc gat atg tcc tgc 144Ile Val Thr Lys Thr Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys
35 40 45acc ggt gct gtg ctc tcc gaa gcc cgc acc att gac gat gtg atc gaa 192Thr Gly Ala Val Leu Ser Glu Ala Arg Thr Ile Asp Asp Val Ile Glu
50 55 60gag atc gcc acc tcc acc ctt acc gaa cgt ggc gca acc cgc gcc gat 240Glu Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp65 70 75 80gac cgc ctc agc gaa tcc gca atg gaa gac cgc aag aag gaa ggc tac 288Asp Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr
85 90 95ttc tgatgactgc tccaaccttg aat 314Phe<210>2<211>97<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>2Pro Pro Ile Tyr Phe Ser His Asp Arg Glu Val Phe Glu Arg Asp Gly1 5 10 15Met Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Lys Lys Gly Glu Glu
20 25 30Ile Val Thr Lys Thr Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys
35 40 45Thr Gly Ala Val Leu Ser Glu Ala Arg Thr Ile Asp Asp Val Ile Glu
50 55 60Glu Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp65 70 75 80Asp Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr
85 90 95Phe<210>3<211>980<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(957)<223>RXN00249<400>3acc ggc gtc tcc acc tcc cag gtt gta gtt ttg ctt gtc gac gcc cgc 48Thr Gly Val Ser Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg1 5 10 15cac ggc gtc gtc gag cag acc cgc cgc cac ctg tcc gta tcg gct ctg 96His Gly Val Val Glu Gln Thr Arg Arg His Leu Ser Val Ser Ala Leu
20 25 30ctg ggc gta cgc acg gtg atc ctc gca gtc aac aaa att gac ctt gtt 144Leu Gly Val Arg Thr Val Ile Leu Ala Val Asn Lys Ile Asp Leu Val
35 40 45gat tac agc gaa gaa gtc ttc cgc aac att gaa aag gaa ttc gtt ggc 192Asp Tyr Ser Glu Glu Val Phe Arg Asn Ile Glu Lys Glu Phe Val Gly
50 55 60ctg gca tct gca ctt gat gtc aca gac acc cac gtt gtt cca atc tct 240Leu Ala Ser Ala Leu Asp Val Thr Asp Thr His Val Val Pro Ile Ser65 70 75 80gcg ctc aag ggc gac aac gtt gca gaa cct tcc acc cac atg gat tgg 288Ala Leu Lys Gly Asp Asn Val Ala Glu Pro Ser Thr His Met Asp Trp
85 90 95tac acc gga cca acc gtg ctg gaa atc ctg gaa aac gta gaa gtt tcc 336Tyr Thr Gly Pro Thr Val Leu Glu Ile Leu Glu Asn Val Glu Val Ser
100 105 110cac ggc cgt gca cac gac ctg ggc ttc cgc ttc cca atc cag tac gtc 384His Gly Arg Ala His Asp Leu Gly Phe Arg Phe Pro Ile Gln Tyr Val
115 120 125atc cgc gag cac gcc acc gac tac cgt ggc tac gcc ggc acc atc aac 432lle Arg Glu His Ala Thr Asp Tyr Arg Gly Tyr Ala Gly Thr Ile Asn
130 135 140gct ggt tcc gtc tcc gtg ggc gat acc gtg tac cta cct gaa ggc cgc 480Ala Gly Ser Val Ser Val Gly Asp Thr Val Tyr Leu Pro Glu Gly Arg145 150 155 160acc acc cag gtc acc cac atc gat tcc gct gac gga tcc ctc cag acc 528Thr Thr Gln Val Thr His Ile Asp Ser Ala Asp Gly Ser Leu Gln Thr
165 170 175gca tca gtt gga gaa gcc gtt gtc ctg cgc cta gcc cag gaa atc gac 576Ala Ser Val Gly Glu Ala Val Val Leu Arg Leu Ala Gln Glu Ile Asp
180 185 190ctc atc cgc ggc gaa ctc atc gct ggc gaa gac cgc cca gaa tcc gtt 624Leu Ile Arg Gly Glu Leu Ile Ala Gly Glu Asp Arg Pro Glu Ser Val
195 200 205cgc tcc ttc aac gcc act gtt gtt ggc ttg gcc gat cgc acc atc aaa 672Arg Ser Phe Asn Ala Thr Val Val Gly Leu Ala Asp Arg Thr Ile Lys
210 215 220cca ggt gca gca gtc aag gtt cgc tac ggc acc gag ctg gtc cgc gga 720Pro Gly Ala Ala Val Lys Val Arg Tyr Gly Thr Glu Leu Val Arg Gly225 230 235 240cgc gtc gca gcc atc gaa cga gtc ctc gac atc gac ggc gtc aac gac 768Arg Val Ala Ala Ile Glu Arg Val Leu Asp Ile Asp Gly Val Asn Asp
245 250 255aac gaa gca cca gaa acc tac ggc ctc aac gac atc gca cac gtg cgc 816Asn Glu Ala Pro Glu Thr Tyr Gly Leu Asn Asp Ile Ala His Val Arg
260 265 270atc gac gtt gca ggc gaa ctc gaa gtt gaa gat tac gct gcc cgc ggc 864Ile Asp Val Ala Gly Glu Leu Glu Val Glu Asp Tyr Ala Ala Arg Gly
275 280 285gcc atc gga tcc ttc ctc ctc atc gac caa tcc tcc ggc gat acc ctc 912Ala Ile Gly Ser Phe Leu Leu Ile Asp Gln Ser Ser Gly Asp Thr Leu
290 295 300gca gct ggc ttg gtt ggc cac cgc cta cgc aat aac tgg tcg atc 957Ala Ala Gly Leu Val Gly His Arg Leu Arg Asn Asn Trp Ser Ile305 310 315tagaccagtg tcttaggcaa gac 980<210>4<211>319<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>4Thr Gly Val Ser Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg1 5 10 15His Gly Val Val Glu Gln Thr Arg Arg His Leu Ser Val Ser Ala Leu
20 25 30Leu Gly Val Arg Thr Val Ile Leu Ala Val Asn Lys Ile Asp Leu Val
35 40 45Asp Tyr Ser Glu Glu Val Phe Arg Asn Ile Glu Lys Glu Phe Val Gly
50 55 60Leu Ala Ser Ala Leu Asp Val Thr Asp Thr His Val Val Pro Ile Ser65 70 75 80Ala Leu Lys Gly Asp Asn Val Ala Glu Pro Ser Thr His Met Asp Trp
85 90 95Tyr Thr Gly Pro Thr Val Leu Glu Ile Leu Glu Asn Val Glu Val Ser
100 105 110His Gly Arg Ala His Asp Leu Gly Phe Arg Phe Pro Ile Gln Tyr Val
115 120 125Ile Arg Glu His Ala Thr Asp Tyr Arg Gly Tyr Ala Gly Thr Ile Asn
130 135 140Ala Gly Ser Val Ser Val Gly Asp Thr Val Tyr Leu Pro Glu Gly Arg145 150 155 160Thr Thr Gln Val Thr His Ile Asp Ser Ala Asp Gly Ser Leu Gln Thr
165 170 175Ala Ser Val Gly Glu Ala Val Val Leu Arg Leu Ala Gln Glu Ile Asp
180 185 190Leu Ile Arg Gly Glu Leu Ile Ala Gly Glu Asp Arg Pro Glu Ser Val
195 200 205Arg Ser Phe Asn Ala Thr Val Val Gly Leu Ala Asp Arg Thr Ile Lys
210 215 220Pro Gly Ala Ala Val Lys Val Arg Tyr Gly Thr Glu Leu Val Arg Gly225 230 235 240Arg Val Ala Ala Ile Glu Arg Val Leu Asp Ile Asp Gly Val Asn Asp
245 250 255Asn Glu Ala Pro Glu Thr Tyr Gly Leu Asn Asp Ile Ala His Val Arg
260 265 270Ile Asp Val Ala Gly Glu Leu Glu Val Glu Asp Tyr Ala Ala Arg Gly
275 280 285Ala Ile Gly Ser Phe Leu Leu Ile Asp Gln Ser Ser Gly Asp Thr Leu
290 295 300Ala Ala Gly Leu Val Gly His Arg Leu Arg Asn Asn Trp Ser Ile305 310 315<210>5<211>977<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(954)<223>FRXA00249<400>5ggt gtt ttc acc ttc caa ggt gta gtt ttg ctt gtc gac gcc cgc cac 48Gly Val Phe Thr Phe Gln Gly Val Val Leu Leu Val Asp Ala Arg His1 5 10 15ggc gtc gtc gag cag acc cgc cgc cac ctg tcc gta tcg gct ctg ctg 96Gly Val Val Glu Gln Thr Arg Arg His Leu Ser Val Ser Ala Leu Leu
20 25 30ggc gta cgc acg gtg atc ctc gca gtc aac aaa att gac ctt gtt gat 144Gly Val Arg Thr Val Ile Leu Ala Val Asn Lys Ile Asp Leu Val Asp
35 40 45tac agc gaa gaa gtc ttc cgc aac att gaa aag gaa ttc gtt ggc ctg 192Tyr Ser Glu Glu Val Phe Arg Asn Ile Glu Lys Glu Phe Val Gly Leu
50 55 60gca tct gca ctt gat gtc aca gac acc cac gtt gtt cca atc tct gcg 240Ala Ser Ala Leu Asp Val Thr Asp Thr His Val Val Pro Ile Ser Ala65 70 75 80ctc aag ggc gac aac gtt gca gaa cct tcc acc cac atg gat tgg tac 288Leu Lys Gly Asp Asn Val Ala Glu Pro Ser Thr His Met Asp Trp Tyr
85 90 95acc gga cca acc gtg ctg gaa atc ctg gaa aac gta gaa gtt tcc cac 336Thr Gly Pro Thr Val Leu Glu Ile Leu Glu Asn Val Glu Val Ser His
100 105 110ggc cgt gca cac gac ctg ggc ttc cgc ttc cca atc cag tac gtc atc 384Gly Arg Ala His Asp Leu Gly Phe Arg Phe Pro Ile Gln Tyr Val Ile
115 120 125cgc gag cac gcc acc gac tac cgt ggc tac gcc ggc acc atc aac gct 432Arg Glu His Ala Thr Asp Tyr Arg Gly Tyr Ala Gly Thr Ile Asn Ala
130 135 140ggt tcc gtc tcc gtg ggc gat acc gtg tac cta cct gaa ggc cgc acc 480Gly Ser Val Ser Val Gly Asp Thr Val Tyr Leu Pro Glu Gly Arg Thr145 150 155 160acc cag gtc acc cac atc gat tcc gct gac gga tcc ctc cag acc gca 528Thr Gln Val Thr His Ile Asp Ser Ala Asp Gly Ser Leu Gln Thr Ala
165 170 175tca gtt gga gaa gcc gtt gtc ctg cgc cta gcc cag gaa atc gac ctc 576Ser Val Gly Glu Ala Val Val Leu Arg Leu Ala Gln Glu Ile Asp Leu
180 185 190atc cgc ggc gaa ctc atc gct ggc gaa gac cgc cca gaa tcc gtt cgc 624Ile Arg Gly Glu Leu Ile Ala Gly Glu Asp Arg Pro Glu Ser Val Arg
195 200 205tcc ttc aac gcc act gtt gtt ggc ttg gcc gat cgc acc atc aaa cca 672Ser Phe Asn Ala Thr Val Val Gly Leu Ala Asp Arg Thr Ile Lys Pro
210 215 220ggt gca gca gtc aag gtt cgc tac ggc acc gag ctg gtc cgc gga cgc 720Gly Ala Ala Val Lys Val Arg Tyr Gly Thr Glu Leu Val Arg Gly Arg225 230 235 240gtc gca gcc atc gaa cga gtc ctc gac atc gac ggc gtc aac gac aac 768Val Ala Ala Ile Glu Arg Val Leu Asp Ile Asp Gly Val Asn Asp Asn
245 250 255gaa gca cca gaa acc tac ggc ctc aac gac atc gca cac gtg cgc atc 816Glu Ala Pro Glu Thr Tyr Gly Leu Asn Asp Ile Ala His Val Arg Ile
260 265 270gac gtt gca ggc gaa ctc gaa gtt gaa gat tac gct gcc cgc ggc gcc 864Asp Val Ala Gly Glu Leu Glu Val Glu Asp Tyr Ala Ala Arg Gly Ala
275 280 285atc gga tcc ttc ctc ctc atc gac caa tcc tcc ggc gat acc ctc gca 912Ile Gly Ser Phe Leu Leu Ile Asp Gln Ser Ser Gly Asp Thr Leu Ala
290 295 300gct ggc ttg gtt ggc cac cgc cta cgc aat aac tgg tcg atc 954Ala Gly Leu Val Gly His Arg Leu Arg Asn Asn Trp Ser Ile305 310 315tagaccagtg tcttaggcaa gac 977<210>6<211>318<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>6Gly Val Phe Thr Phe Gln Gly Val Val Leu Leu Val Asp Ala Arg His1 5 10 15Gly Val Val Glu Gln Thr Arg Arg His Leu Ser Val Ser Ala Leu Leu
20 25 30Gly Val Arg Thr Val Ile Leu Ala Val Asn Lys Ile Asp Leu Val Asp
35 40 45Tyr Ser Glu Glu Val Phe Arg Asn Ile Glu Lys Glu Phe Val Gly Leu
50 55 60Ala Ser Ala Leu Asp Val Thr Asp Thr His Val Val Pro Ile Ser Ala65 70 75 80Leu Lys Gly Asp Asn Val Ala Glu Pro Ser Thr His Met Asp Trp Tyr
85 90 95Thr Gly Pro Thr Val Leu Glu Ile Leu Glu Asn Val Glu Val Ser His
100 105 110Gly Arg Ala His Asp Leu Gly Phe Arg Phe Pro Ile Gln Tyr Val Ile
115 120 125Arg Glu His Ala Thr Asp Tyr Arg Gly Tyr Ala Gly Thr Ile Asn Ala
130 135 140Gly Ser Val Ser Val Gly Asp Thr Val Tyr Leu Pro Glu Gly Arg Thr145 150 155 160Thr Gln Val Thr His Ile Asp Ser Ala Asp Gly Ser Leu Gln Thr Ala
165 170 175Ser Val Gly Glu Ala Val Val Leu Arg Leu Ala Gln Glu Ile Asp Leu
180 185 190Ile Arg Gly Glu Leu Ile Ala Gly Glu Asp Arg Pro Glu Ser Val Arg
195 200 205Ser Phe Asn Ala Thr Val Val Gly Leu Ala Asp Arg Thr Ile Lys Pro
210 215 220Gly Ala Ala Val Lys Val Arg Tyr Gly Thr Glu Leu Val Arg Gly Arg225 230 235 240Val Ala Ala Ile Glu Arg Val Leu Asp Ile Asp Gly Val Asn Asp Asn
245 250 255Glu Ala Pro Glu Thr Tyr Gly Leu Asn Asp Ile Ala His Val Arg Ile
260 265 270Asp Val Ala Gly Glu Leu Glu Val Glu Asp Tyr Ala Ala Arg Gly Ala
275 280 285Ile Gly Ser Phe Leu Leu Ile Asp Gln Ser Ser Gly Asp Thr Leu Ala
290 295 300Ala Gly Leu Val Gly His Arg Leu Arg Asn Asn Trp Ser Ile305 310 315<210>7<211>954<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(931)<223>RXA01073<400>7taaccgactc cagcactaaa ctccaaaccc ttggcccgca ccgccaaagt ttagcgcgcc 60ccaagacacc accgcgccat gtttgcctag gattaggtac atg aca aac act caa 115
Met Thr Asn Thr Gln
1 5acc gag atc att aat gaa cta aag gtg agc cca gca atc gac gtg gcc 163Thr Glu Ile Ile Asn Glu Leu Lys Val Ser Pro Ala Ile Asp Val Ala
10 15 20aag gaa gtt gaa ttc cgt gtg cag ttc ctc gtc gat tac ctg cgg gct 211Lys Glu Val Glu Phe Arg Val Gln Phe Leu Val Asp Tyr Leu Arg Ala
25 30 35tcc cat aca aaa ggc ttt gtt ctt ggt att tca ggt ggc cag gat tcc 259Ser His Thr Lys Gly Phe Val Leu Gly Ile Ser Gly Gly Gln Asp Ser
40 45 50act ctt gcg gga cga ctc acg cag ctg gca gta gag cgc att cgt gcg 307Thr Leu Ala Gly Arg Leu Thr Gln Leu Ala Val Glu Arg Ile Arg Ala
55 60 65gaa gaa aac agc acg gat tat gtc ttc tac gca gtt cgc ctc ccc tac 355Glu Glu Asn Ser Thr Asp Tyr Val Phe Tyr Ala Val Arg Leu Pro Tyr70 75 80 85gcg atc cag gca gat gag gac gat gcg caa gtt gca ttg gaa ttc atc 403Ala Ile Gln Ala Asp Glu Asp Asp Ala Gln Val Ala Leu Glu Phe Ile
90 95 100gca cct gac aag agc gtg acc gtc aac gtt aaa gac gca acg gac gcc 451Ala Pro Asp Lys Ser Val Thr Val Asn Val Lys Asp Ala Thr Asp Ala
105 110 115acc gaa gca act gtt gca gct gct ttg gaa ctt cct gag ctg acc gac 499Thr Glu Ala Thr Val Ala Ala Ala Leu Glu Leu Pro Glu Leu Thr Asp
120 125 130ttc aat cgg ggc aat att aaa gct cgc caa cgc atg gtt gcc cag tac 547Phe Asn Arg Gly Asn Ile Lys Ala Arg Gln Arg Met Val Ala Gln Tyr
135 140 145gca atc gca ggc cag ttg ggc ttg ctg gtt att ggc act gat cac gcg 595Ala Ile Ala Gly Gln Leu Gly Leu Leu Val Ile Gly Thr Asp His Ala150 155 160 165gct gaa aac gtc acg ggg ttc ttc acc aaa ttc ggt gat ggc gca gct 643Ala Glu Asn Val Thr Gly Phe Phe Thr Lys Phe Gly Asp Gly Ala Ala
170 175 180gac ctg ctt cct ttg gca ggt ttg agc aag cgt caa gga gct gcc att 691Asp Leu Leu Pro Leu Ala Gly Leu Ser Lys Arg Gln Gly Ala Ala Ile
185 190 195ctg gag cac ctg ggt gca cct tca agc acg tgg acc aag gtt cct acc 739Leu Glu His Leu Gly Ala Pro Ser Ser Thr Trp Thr Lys Val Pro Thr
200 205 210gct gat ttg gaa gag gat cgc cca gcg ttg cca gat gag gaa gca ctt 787Ala Asp Leu Glu Glu Asp Arg Pro Ala Leu Pro Asp Glu Glu Ala Leu
215 220 225ggt gtg tcg tat gcg gac atc gat aat tac ctg gaa aac aag ccc gat 835Gly Val Ser Tyr Ala Asp Ile Asp Asn Tyr Leu Glu Asn Lys Pro Asp230 235 240 245gtc agt gaa aaa gcc cag cag cgc att gag cac ctg tgg aag gtg ggc 883Val Ser Glu Lys Ala Gln Gln Arg Ile Glu His Leu Trp Lys Val Gly
250 255 260cag cac aag cgc cac ctc cct gct acc ccg cag gaa aat tgg tgg cgt 931Gln His Lys Arg His Leu Pro Ala Thr Pro Gln Glu Asn Trp Trp Arg
265 270 275taatccaaca gtttgagtgt cgc 954<210>8<211>277<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>8Met Thr Asn Thr Gln Thr Glu Ile Ile Asn Glu Leu Lys Val Ser Pro1 5 10 15Ala Ile Asp Val Ala Lys Glu Val Glu Phe Arg Val Gln Phe Leu Val
20 25 30Asp Tyr Leu Arg Ala Ser His Thr Lys Gly Phe Val Leu Gly Ile Ser
35 40 45Gly Gly Gln Asp Ser Thr Leu Ala Gly Arg Leu Thr Gln Leu Ala Val
50 55 60Glu Arg Ile Arg Ala Glu Glu Asn Ser Thr Asp Tyr Val Phe Tyr Ala65 70 75 80Val Arg Leu Pro Tyr Ala Ile Gln Ala Asp Glu Asp Asp Ala Gln Val
85 90 95Ala Leu Glu Phe Ile Ala Pro Asp Lys Ser Val Thr Val Asn Val Lys
100 105 110Asp Ala Thr Asp Ala Thr Glu Ala Thr Val Ala Ala Ala Leu Glu Leu
115 120 125Pro Glu Leu Thr Asp Phe Asn Arg Gly Asn Ile Lys Ala Arg Gln Arg
130 135 140Met Val Ala Gln Tyr Ala Ile Ala Gly Gln Leu Gly Leu Leu Val Ile145 150 155 160Gly Thr Asp His Ala Ala Glu Asn Val Thr Gly Phe Phe Thr Lys Phe
165 170 175Gly Asp Gly Ala Ala Asp Leu Leu Pro Leu Ala Gly Leu Ser Lys Arg
180 185 190Gln Gly Ala Ala Ile Leu Glu His Leu Gly Ala Pro Ser Ser Thr Trp
195 200 205Thr Lys Val Pro Thr Ala Asp Leu Glu Glu Asp Arg Pro Ala Leu Pro
210 215 220Asp Glu Glu Ala Leu Gly Val Ser Tyr Ala Asp Ile Asp Asn Tyr Leu225 230 235 240Glu Asn Lys Pro Asp Val Ser Glu Lys Ala Gln Gln Arg Ile Glu His
245 250 255Leu Trp Lys Val Gly Gln His Lys Arg His Leu Pro Ala Thr Pro Gln
260 265 270Glu Asn Trp Trp Arg
275<210>9<211>609<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(586)<223>RXN02913<400>9tccctgacat ccaggttgaa gcaacgtttg atgacggcac caagctcgtc accgtgcaca 60atcccatccg ataacccttg atgtttttag gagttttgtc atg atc cca ggc gag 115
Met Ile Pro Gly Glu
1 5tac atc ctg tcc agc gaa tca ctc acc gga aat gtt ggg cgc gag gcc 163Tyr Ile Leu Ser Ser Glu Ser Leu Thr Gly Asn Val Gly Arg Glu Ala
10 15 20aaa acc atc gaa atc atc aac acc ggt gat agg cct gtg cag att ggt 211Lys Thr Ile Glu Ile Ile Asn Thr Gly Asp Arg Pro Val Gln Ile Gly
25 30 35tcg cat ttc cac ttc gct gaa gta aac ccc agc atc agc ttt gat cgc 259Ser His Phe His Phe Ala Glu Val Asn Pro Ser Ile Ser Phe Asp Arg
40 45 50agt gaa ggt tac ggc ttc cgc ctt gat att cca tcc ggc acc gcg gtg 307Ser Glu Gly Tyr Gly Phe Arg Leu Asp Ile Pro Ser Gly Thr Ala Val
55 60 65cgc ctc gag cca ggc gat gcc cgc acc gtc aac ctt gtt gcc att ggc 355Arg Leu Glu Pro Gly Asp Ala Arg Thr Val Asn Leu Val Ala Ile Gly70 75 80 85ggt gac cgc att gtt gca ggt ttc cga gat ctc gtc gat ggg ccg ttg 403Gly Asp Arg Ile Val Ala Gly Phe Arg Asp Leu Val Asp Gly Pro Leu
90 95 100gag gac ctc aaa gtc aac gtg tgg gag gga cgc gaa gac ggt tgg cgt 451Glu Asp Leu Lys Val Asn Val Trp Glu Gly Arg Glu Asp Gly Trp Arg
105 110 115cgt tcc tca gct gct ggt gat gct cca caa gaa ttg cca cag gtc gaa 499Arg Ser Ser Ala Ala Gly Asp Ala Pro Gln Glu Leu Pro Gln Val Glu
120 125 130gct gct gaa cgt ggc cgg aaa cta gat gac gcc act gat gtg gac aca 547Ala Ala Glu Arg Gly Arg Lys Leu Asp Asp Ala Thr Asp Val Asp Thr
135 140 145aat gtg ggc aca gaa gaa ggc ttt gaa gaa ggt cga aat taaatgagtt 596Asn Val Gly Thr Glu Glu Gly Phe Glu Glu Gly Arg Asn150 155 160ttgagatttc ccg 609<210>10<211>162<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>10Met Ile Pro Gly Glu Tyr Ile Leu Ser Ser Glu Ser Leu Thr Gly Asn1 5 10 15Val Gly Arg Glu Ala Lys Thr Ile Glu Ile Ile Asn Thr Gly Asp Arg
20 25 30Pro Val Gln Ile Gly Ser His Phe His Phe Ala Glu Val Asn Pro Ser
35 40 45Ile Ser Phe Asp Arg Ser Glu Gly Tyr Gly Phe Arg Leu Asp Ile Pro
50 55 60Ser Gly Thr Ala Val Arg Leu Glu Pro Gly Asp Ala Arg Thr Val Asn65 70 75 80Leu Val Ala Ile Gly Gly Asp Arg Ile Val Ala Gly Phe Arg Asp Leu
85 90 95Val Asp Gly Pro Leu Glu Asp Leu Lys Val Asn Val Trp Glu Gly Arg
100 105 110Glu Asp Gly Trp Arg Arg Ser Ser Ala Ala Gly Asp Ala Pro Gln Glu
115 120 125Leu Pro Gln Val Glu Ala Ala Glu Arg Gly Arg Lys Leu Asp Asp Ala
l30 135 140Thr Asp Val Asp Thr Asn Val Gly Thr Glu Glu Gly Phe Glu Glu Gly145 150 155 160Arg Asn<210>11<211>220<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(220)<223>FRXA02264<400>11tccctgacat ccaggttgaa gcaacgtttg atgacggcac caagctcgtc accgtgcaca 60atcccatccg ataacccttg atgtttttag gagttttgtc atg atc cca ggc gag 115
Met Ile Pro Gly Glu
1 5tac atc ctg tcc agc gaa tca ctc acc gga aat gtt ggg cgc gag gcc 163Tyr Ile Leu Ser Ser Glu Ser Leu Thr Gly Asn Val Gly Arg Glu Ala
10 15 20aaa acc atc gaa atc atc aac acc ggt gat agg cct gtg cag att ggt 211Lys Thr Ile Glu Ile Ile Asn Thr Gly Asp Arg Pro Val Gln Ile Gly
25 30 35tcg cat ttc 220Ser His Phe
40<210>12<211>40<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>12Met Ile Pro Gly Glu Tyr Ile Leu Ser Ser Glu Ser Leu Thr Gly Asn1 5 10 15Val Gly Arg Glu Ala Lys Thr Ile Glu Ile Ile Asn Thr Gly Asp Arg
20 25 30Pro Val Gln Ile Gly Ser His Phe
35 40<210>13<211>1833<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1810)<223>RXN02274<400>13acaggtcgaa gctgctgaac gtggccggaa actagatgac gccactgatg tggacacaaa 60tgtgggcaca gaagaaggct ttgaagaagg tcgaaattaa atg agt ttt gag att 115
Met Ser Phe Glu Ile
1 5tcc cgc aag cag tac acc gac ctt tat ggt cca acc gtt ggc gat tca 163Ser Arg Lys Gln Tyr Thr Asp Leu Tyr Gly Pro Thr Val Gly Asp Ser
10 15 20gta cgt ctt gct gat act gag ctt ttt ctc tgt gtg gaa aaa gat tac 211Val Arg Leu Ala Asp Thr Glu Leu Phe Leu Cys Val Glu Lys Asp Tyr
25 30 35gca gca atc ggc gaa gaa gta gca ttc ggc ggt ggc aag gtc att cgt 259Ala Ala Ile Gly Glu Glu Val Ala Phe Gly Gly Gly Lys Val Ile Arg
40 45 50gat ggc atg ggc caa aat ggc acc ttg gtt cgc gat gta gat att ccc 307Asp Gly Met Gly Gln Asn Gly Thr Leu Val Arg Asp Val Asp Ile Pro
55 60 65gat acc gtc atc acc aac gtc atc gtc ctt gac tat acg ggt gtg tac 355Asp Thr Val Ile Thr Asn Val Ile Val Leu Asp Tyr Thr Gly Val Tyr70 75 80 85aaa gct gac gtt gcg ctt cga gat ggc aaa atc ttc cga atc gga aag 403Lys Ala Asp Val Ala Leu Arg Asp Gly Lys Ile Phe Arg Ile Gly Lys
90 95 100gcc gga aac ccg aat gtc atg gaa aac gtc gac atc gtc atc ggc gtt 451Ala Gly Asn Pro Asn Val Met Glu Asn Val Asp Ile Val Ile Gly Val
105 110 115gcc acc gac atc att gct ggt gaa ggc aaa atc ctt acc gca ggt ggc 499Ala Thr Asp Ile Ile Ala Gly Glu Gly Lys Ile Leu Thr Ala Gly Gly
120 125 130atc gac acg cac gtg cac ttc ttg ggc aca gac cag gtc aac act gca 547Ile Asp Thr His Val His Phe Leu Gly Thr Asp Gln Val Asn Thr Ala
135 140 145tta gca tca ggt atc acc acg atg atc ggt gga ggc acc ggc cca agc 595Leu Ala Ser Gly Ile Thr Thr Met Ile Gly Gly Gly Thr Gly Pro Ser150 155 160 165cag gcg tcg atg gct aca act gtc acg cca ggt cag tgg aat acc tac 643Gln Ala Ser Met Ala Thr Thr Val Thr Pro Gly Gln Trp Asn Thr Tyr
170 175 180aac atg ctt agt gct ttt gaa ggc atg ccc atg aac ttt ggc att ttg 691Asn Met Leu Ser Ala Phe Glu Gly Met Pro Met Asn Phe Gly Ile Leu
185 190 195ggt aaa ggc cat ggt tct tcc aaa tct ccg ctg gct gag cag gtt cgt 739Gly Lys Gly His Gly Ser Ser Lys Ser Pro Leu Ala Glu Gln Val Arg
200 205 210gcg ggt gca atc ggt ctg aaa att cac gag gac tgg ggt gcc aca cca 787Ala Gly Ala Ile Gly Leu Lys Ile His Glu Asp Trp Gly Ala Thr Pro
215 220 225tcg tcg atc aac act gcc cta gaa gta gcc gat gac atg gac atc cag 835Ser Ser Ile Asn Thr Ala Leu Glu Val Ala Asp Asp Met Asp Ile Gln230 235 240 245gtg gca ctc cac tcc gat acc ttg aat gag gcc ggt ttt gtg gaa gac 883Val Ala Leu His Ser Asp Thr Leu Asn Glu Ala Gly Phe Val Glu Asp
250 255 260acc att gaa gcc att gcg ggc cga gtc atc cat acc ttc cac acc gaa 931Thr Ile Glu Ala Ile Ala Gly Arg Val Ile His Thr Phe His Thr Glu
265 270 275ggt gct ggt ggt gga cac gct cct gac cta atc cga gtg gct gct ctg 979Gly Ala Gly Gly Gly His Ala Pro Asp Leu Ile Arg Val Ala Ala Leu
280 285 290cca aac gtg ttg cct gca tcc acc aac cca acg ctc cca tac acc cga 1027Pro Asn Val Leu Pro Ala Ser Thr Asn Pro Thr Leu Pro Tyr Thr Arg
295 300 305aac act gtt gaa gag cac ctg gac atg gtg atg gtt gcc cac cac ctc 1075Asn Thr Val Glu Glu His Leu Asp Met Val Met Val Ala His His Leu310 315 320 325aac cca gat att cca gaa gac gtg gct ttt gcg gat tcc cga att cgt 1123Asn Pro Asp Ile Pro Glu Asp Val Ala Phe Ala Asp Ser Arg Ile Arg
330 335 340gcc gaa acg att gca gcc gaa gat gtg ctt cac gat atg ggt atc ttc 1171Ala Glu Thr Ile Ala Ala Glu Asp Val Leu His Asp Met Gly Ile Phe
345 350 355tct atc acc tct tcg gat tcc cag gcg atg ggc cga gta gga gag acc 1219Ser Ile Thr Ser Ser Asp Ser Gln Ala Met Gly Arg Val Gly Glu Thr
360 365 370atc acg cgc acg tgg cag gtc gcc gac cat atg aaa cgc acc cgt gga 1267Ile Thr Arg Thr Trp Gln Val Ala Asp His Met Lys Arg Thr Arg Gly
375 380 385tca cta acg gga gat gct cca tac aac gac aac aac cgc ttg cgt cga 1315Ser Leu Thr Gly Asp Ala Pro Tyr Asn Asp Asn Asn Arg Leu Arg Arg390 395 400 405ttc atc gca aaa tac acc atc aac cct gcg att gcg cac ggt gtg gat 1363Phe Ile Ala Lys Tyr Thr Ile Asn Pro Ala Ile Ala His Gly Val Asp
410 415 420tat gtt gtt cgt tca gtg gag gaa ggc aag ttc gct gac ctc gtg ctg 1411Tyr Val Val Arg Ser Val Glu Glu Gly Lys Phe Ala Asp Leu Val Leu
425 430 435tgg gat cca aag ttc ttt ggt gtg aaa cct gat ctg gtg atc aag ggt 1459Trp Asp Pro Lys Phe Phe Gly Val Lys Pro Asp Leu Val Ile Lys Gly
440 445 450ggg ttg atg gtc aat tcc ctc atg ggt gat tcc aac ggt tcc att cca 1507Gly Leu Met Val Asn Ser Leu Met Gly Asp Ser Asn Gly Ser Ile Pro
455 460 465act ccg cag ccc cgc acc ctg cgc aat act tgg ggt gcg ttt ggc cag 1555Thr Pro Gln Pro Arg Thr Leu Arg Asn Thr Trp Gly Ala Phe Gly Gln470 475 480 485gca gtt tcc aga agc tcc att aca ttc cta tcc cag gac gct atc gat 1603Ala Val Ser Arg Ser Ser Ile Thr Phe Leu Ser Gln Asp Ala Ile Asp
490 495 500gca aat gtt cct gat ctg ctg aat ctg agg aag cag atc cgg ggc gtt 1651Ala Asn Val Pro Asp Leu Leu Asn Leu Arg Lys Gln Ile Arg Gly Val
505 510 515cga ggt gta agg aat ctg acc aaa cga gac atg aaa ctc aat gca gaa 1699Arg Gly Val Arg Asn Leu Thr Lys Arg Asp Met Lys Leu Asn Ala Glu
520 525 530atg cct gat att cgt gtc gat cca gag acc tac cag gtg ttt gtc aac 1747Met Pro Asp Ile Arg Val Asp Pro Glu Thr Tyr Gln Val Phe Val Asn
535 540 545ggt gag ttg atc acc agc aag cca gca gag aca gtg cca atg gca cgt l795Gly Glu Leu Ile Thr Ser Lys Pro Ala Glu Thr Val Pro Met Ala Arg550 555 560 565cgc tac ttc ttg ttc taatccgcca acaaggaagg aag 1833Arg Tyr Phe Leu Phe
570<210>14<211>570<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>14Met Ser Phe Glu Ile Ser Arg Lys Gln Tyr Thr Asp Leu Tyr Gly Pro1 5 10 15Thr Val Gly Asp Ser Val Arg Leu Ala Asp Thr Glu Leu Phe Leu Cys
20 25 30Val Glu Lys Asp Tyr Ala Ala Ile Gly Glu Glu Val Ala Phe Gly Gly
35 40 45Gly Lys Val Ile Arg Asp Gly Met Gly Gln Asn Gly Thr Leu Val Arg
50 55 60Asp Val Asp Ile Pro Asp Thr Val Ile Thr Asn Val Ile Val Leu Asp65 70 75 80Tyr Thr Gly Val Tyr Lys Ala Asp Val Ala Leu Arg Asp Gly Lys Ile
85 90 95Phe Arg Ile Gly Lys Ala Gly Asn Pro Asn Val Met Glu Asn Val Asp
100 105 110Ile Val Ile Gly Val Ala Thr Asp Ile Ile Ala Gly Glu Gly Lys Ile
115 120 125Leu Thr Ala Gly Gly Ile Asp Thr His Val His Phe Leu Gly Thr Asp
130 135 140Gln Val Asn Thr Ala Leu Ala Ser Gly Ile Thr Thr Met Ile Gly Gly145 150 155 160Gly Thr Gly Pro Ser Gln Ala Ser Met Ala Thr Thr Val Thr Pro Gly
165 170 175Gln Trp Asn Thr Tyr Asn Met Leu Ser Ala Phe Glu Gly Met Pro Met
180 185 190Asn Phe Gly Ile Leu Gly Lys Gly His Gly Ser Ser Lys Ser Pro Leu
195 200 205Ala Glu Gln Val Arg Ala Gly Ala Ile Gly Leu Lys Ile His Glu Asp
210 215 220Trp Gly Ala Thr Pro Ser Ser Ile Asn Thr Ala Leu Glu Val Ala Asp225 230 235 240Asp Met Asp Ile Gln Val Ala Leu His Ser Asp Thr Leu Asn Glu Ala
245 250 255Gly Phe Val Glu Asp Thr Ile Glu Ala Ile Ala Gly Arg Val Ile His
260 265 270Thr Phe His Thr Glu Gly Ala Gly Gly Gly His Ala Pro Asp Leu Ile
275 280 285Arg Val Ala Ala Leu Pro Asn Val Leu Pro Ala Ser Thr Asn Pro Thr
290 295 300Leu Pro Tyr Thr Arg Asn Thr Val Glu Glu His Leu Asp Met Val Met305 310 315 320Val Ala His His Leu Asn Pro Asp Ile Pro Glu Asp Val Ala Phe Ala
325 330 335Asp Ser Arg Ile Arg Ala Glu Thr Ile Ala Ala Glu Asp Val Leu His
340 345 350Asp Met Gly Ile Phe Ser Ile Thr Ser Ser Asp Ser Gln Ala Met Gly
355 360 365Arg Val Gly Glu Thr Ile Thr Arg Thr Trp Gln Val Ala Asp His Met
370 375 380Lys Arg Thr Arg Gly Ser Leu Thr Gly Asp Ala Pro Tyr Asn Asp Asn385 390 395 400Asn Arg Leu Arg Arg Phe Ile Ala Lys Tyr Thr Ile Asn Pro Ala Ile
405 410 415Ala His Gly Val Asp Tyr Val Val Arg Ser Val Glu Glu Gly Lys Phe
420 425 430Ala Asp Leu Val Leu Trp Asp Pro Lys Phe Phe Gly Val Lys Pro Asp
435 440 445Leu Val Ile Lys Gly Gly Leu Met Val Asn Ser Leu Met Gly Asp Ser
450 455 460Asn Gly Ser Ile Pro Thr Pro Gln Pro Arg Thr Leu Arg Asn Thr Trp465 470 475 480Gly Ala Phe Gly Gln Ala Val Ser Arg Ser Ser Ile Thr Phe Leu Ser
485 490 495Gln Asp Ala Ile Asp Ala Asn Val Pro Asp Leu Leu Asn Leu Arg Lys
500 505 510Gln Ile Arg Gly Val Arg Gly Val Arg Asn Leu Thr Lys Arg Asp Met
515 520 525Lys Leu Asn Ala Glu Met Pro Asp Ile Arg Val Asp Pro Glu Thr Tyr
530 535 540Gln Val Phe Val Asn Gly Glu Leu Ile Thr Ser Lys Pro Ala Glu Thr545 550 555 560Val Pro Met Ala Arg Arg Tyr Phe Leu Phe
565 570<210>15<211>1625<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(1602)<223>FRXA02274<400>15tac gca gca atc ggc gaa gaa gta gca ttc ggc ggt ggc aag gtc att 48Tyr Ala Ala Ile Gly Glu Glu Val Ala Phe Gly Gly Gly Lys Val Ile1 5 10 15cgt gat ggc atg ggc caa aat ggc acc ttg gtt cgc gat gta gat att 96Arg Asp Gly Met Gly Gln Asn Gly Thr Leu Val Arg Asp Val Asp Ile
20 25 30ccc gat acc gtc atc acc aac gtc atc gtc ctt gac tat acg ggt gtg 144Pro Asp Thr Val Ile Thr Asn Val Ile Val Leu Asp Tyr Thr Gly Val
35 40 45tac aaa gct gac gtt gcg ctt cga gat ggc aaa atc ttc cga atc gga 192Tyr Lys Ala Asp Val Ala Leu Arg Asp Gly Lys Ile Phe Arg Ile Gly
50 55 60aag gcc gga aac ccg aat gtc atg gaa aac gtc gac atc gtc atc ggc 240Lys Ala Gly Asn Pro Asn Val Met Glu Asn Val Asp Ile Val Ile Gly65 70 75 80gtt gcc acc gac atc att gct ggt gaa ggc aaa atc ctt acc gca ggt 288Val Ala Thr Asp Ile Ile Ala Gly Glu Gly Lys Ile Leu Thr Ala Gly
85 90 95ggc atc gac acg cac gtg cac ttc ttg ggc aca gac cag gtc aac act 336Gly Ile Asp Thr His Val His Phe Leu Gly Thr Asp Gln Val Asn Thr
100 105 110gca tta gca tca ggt atc acc acg atg atc ggt gga ggc acc ggc cca 384Ala Leu Ala Ser Gly Ile Thr Thr Met Ile Gly Gly Gly Thr Gly Pro
115 120 125agc cag gcg tcg atg gct aca act gtc acg cca ggt cag tgg aat acc 432Ser Gln Ala Ser Met Ala Thr Thr Val Thr Pro Gly Gln Trp Asn Thr
130 135 140tac aac atg ctt agt gct ttt gaa ggc atg ccc atg aac ttt ggc att 480Tyr Asn Met Leu Ser Ala Phe Glu Gly Met Pro Met Asn Phe Gly Ile145 150 155 160ttg ggt aaa ggc cat ggt tct tcc aaa tct ccg ctg gct gag cag gtt 528Leu Gly Lys Gly His Gly Ser Ser Lys Ser Pro Leu Ala Glu Gln Val
165 170 175cgt gcg ggt gca atc ggt ctg aaa att cac gag gac tgg ggt gcc aca 576Arg Ala Gly Ala Ile Gly Leu Lys Ile His Glu Asp Trp Gly Ala Thr
180 185 190cca tcg tcg atc aac act gcc cta gaa gta gcc gat gac atg gac atc 624Pro Ser Ser Ile Asn Thr Ala Leu Glu Val Ala Asp Asp Met Asp Ile
195 200 205cag gtg gca ctc cac tcc gat acc ttg aat gag gcc ggt ttt gtg gaa 672Gln Val Ala Leu His Ser Asp Thr Leu Asn Glu Ala Gly Phe Val Glu
210 215 220gac acc att gaa gcc att gcg ggc cga gtc atc cat acc ttc cac acc 720Asp Thr Ile Glu Ala Ile Ala Gly Arg Val Ile His Thr Phe His Thr225 230 235 240gaa ggt gct ggt ggt gga cac gct cct gac cta atc cga gtg gct gct 768Glu Gly Ala Gly Gly Gly His Ala Pro Asp Leu Ile Arg Val Ala Ala
245 250 255ctg cca aac gtg ttg cct gca tcc acc aac cca acg ctc cca tac acc 816Leu Pro Asn Val Leu Pro Ala Ser Thr Asn Pro Thr Leu Pro Tyr Thr
260 265 270cga aac act gtt gaa gag cac ctg gac atg gtg atg gtt gcc cac cac 864Arg Asn Thr Val Glu Glu His Leu Asp Met Val Met Val Ala His His
275 280 285ctc aac cca gat att cca gaa gac gtg gct ttt gcg gat tcc cga att 912Leu Asn Pro Asp Ile Pro Glu Asp Val Ala Phe Ala Asp Ser Arg Ile
290 295 300cgt gcc gaa acg att gca gcc gaa gat gtg ctt cac gat atg ggt atc 960Arg Ala Glu Thr Ile Ala Ala Glu Asp Val Leu His Asp Met Gly Ile305 310 315 320ttc tct atc acc tct tcg gat tcc cag gcg atg ggc cga gta gga gag 1008Phe Ser Ile Thr Ser Ser Asp Ser Gln Ala Met Gly Arg Val Gly Glu
325 330 335acc atc acg cgc acg tgg cag gtc gcc gac cat atg aaa cgc acc cgt 1056Thr Ile Thr Arg Thr Trp Gln Val Ala Asp His Met Lys Arg Thr Arg
340 345 350gga tca cta acg gga gat gct cca tac aac gac aac aac cgc ttg cgt 1104Gly Ser Leu Thr Gly Asp Ala Pro Tyr Asn Asp Asn Asn Arg Leu Arg
355 360 365cga ttc atc gca aaa tac acc atc aac cct gcg att gcg cac ggt gtg 1152Arg Phe Ile Ala Lys Tyr Thr Ile Asn Pro Ala Ile Ala His Gly Val
370 375 380gat tat gtt gtt cgt tca gtg gag gaa ggc aag ttc gct gac ctc gtg 1200Asp Tyr Val Val Arg Ser Val Glu Glu Gly Lys Phe Ala Asp Leu Val385 390 395 400ctg tgg gat cca aag ttc ttt ggt gtg aaa cct gat ctg gtg atc aag 1248Leu Trp Asp Pro Lys Phe Phe Gly Val Lys Pro Asp Leu Val Ile Lys
405 410 415ggt ggg ttg atg gtc aat tcc ctc atg ggt gat tcc aac ggt tcc att 1296Gly Gly Leu Met Val Asn Ser Leu Met Gly Asp Ser Asn Gly Ser Ile
420 425 430cca act ccg cag ccc cgc acc ctg cgc aat act tgg ggt gcg ttt ggc 1344Pro Thr Pro Gln Pro Arg Thr Leu Arg Asn Thr Trp Gly Ala Phe Gly
435 440 445cag gca gtt tcc aga agc tcc att aca ttc cta tcc cag gac gct atc 1392Gln Ala Val Ser Arg Ser Ser Ile Thr Phe Leu Ser Gln Asp Ala Ile
450 455 460gat gca aat gtt cct gat ctg ctg aat ctg agg aag cag atc cgg ggc 1440Asp Ala Asn Val Pro Asp Leu Leu Asn Leu Arg Lys Gln Ile Arg Gly465 470 475 480gtt cga ggt gta agg aat ctg acc aaa cga gac atg aaa ctc aat gca 1488Val Arg Gly Val Arg Asn Leu Thr Lys Arg Asp Met Lys Leu Asn Ala
485 490 495gaa atg cct gat att cgt gtc gat cca gag acc tac cag gtg ttt gtc 1536Glu Met Pro Asp Ile Arg Val Asp Pro Glu Thr Tyr Gln Val Phe Val
500 505 510aac ggt gag ttg atc acc agc aag cca gca gag aca gtg cca atg gca 1584Asn Gly Glu Leu Ile Thr Ser Lys Pro Ala Glu Thr Val Pro Met Ala
515 520 525cgt cgc tac ttc ttg ttc taatccgcca acaaggaagg aag 1625Arg Arg Tyr Phe Leu Phe
530<210>16<211>534<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>16Tyr Ala Ala Ile Gly Glu Glu Val Ala Phe Gly Gly Gly Lys Val Ile1 5 10 15Arg Asp Gly Met Gly Gln Asn Gly Thr Leu Val Arg Asp Val Asp Ile
20 25 30Pro Asp Thr Val Ile Thr Asn Val Ile Val Leu Asp Tyr Thr Gly Val
35 40 45Tyr Lys Ala Asp Val Ala Leu Arg Asp Gly Lys Ile Phe Arg Ile Gly
50 55 60Lys Ala Gly Asn Pro Asn Val Met Glu Asn Val Asp Ile Val Ile Gly65 70 75 80Val Ala Thr Asp Ile Ile Ala Gly Glu Gly Lys Ile Leu Thr Ala Gly
85 90 95Gly Ile Asp Thr His Val His Phe Leu Gly Thr Asp Gln Val Asn Thr
100 105 110Ala Leu Ala Ser Gly Ile Thr Thr Met Ile Gly Gly Gly Thr Gly Pro
115 120 125Ser Gln Ala Ser Met Ala Thr Thr Val Thr Pro Gly Gln Trp Asn Thr
130 135 140Tyr Asn Met Leu Ser Ala Phe Glu Gly Met Pro Met Asn Phe Gly Ile145 150 155 160Leu Gly Lys Gly His Gly Ser Ser Lys Ser Pro Leu Ala Glu Gln Val
165 170 175Arg Ala Gly Ala Ile Gly Leu Lys Ile His Glu Asp Trp Gly Ala Thr
180 185 190Pro Ser Ser Ile Asn Thr Ala Leu Glu Val Ala Asp Asp Met Asp Ile
195 200 205Gln Val Ala Leu His Ser Asp Thr Leu Asn Glu Ala Gly Phe Val Glu
210 215 220Asp Thr Ile Glu Ala Ile Ala Gly Arg Val Ile His Thr Phe His Thr225 230 235 240Glu Gly Ala Gly Gly Gly His Ala Pro Asp Leu Ile Arg Val Ala Ala
245 250 255Leu Pro Asn Val Leu Pro Ala Ser Thr Asn Pro Thr Leu Pro Tyr Thr
260 265 270Arg Asn Thr Val Glu Glu His Leu Asp Met Val Met Val Ala His His
275 280 285Leu Asn Pro Asp Ile Pro Glu Asp Val Ala Phe Ala Asp Ser Arg Ile
290 295 300Arg Ala Glu Thr Ile Ala Ala Glu Asp Val Leu His Asp Met Gly Ile305 310 315 320Phe Ser Ile Thr Ser Ser Asp Ser Gln Ala Met Gly Arg Val Gly Glu
325 330 335Thr Ile Thr Arg Thr Trp Gln Val Ala Asp His Met Lys Arg Thr Arg
340 345 350Gly Ser Leu Thr Gly Asp Ala Pro Tyr Asn Asp Asn Asn Arg Leu Arg
355 360 365Arg Phe Ile Ala Lys Tyr Thr Ile Asn Pro Ala Ile Ala His Gly Val
370 375 380Asp Tyr Val Val Arg Ser Val Glu Glu Gly Lys Phe Ala Asp Leu Val385 390 395 400Leu Trp Asp Pro Lys Phe Phe Gly Val Lys Pro Asp Leu Val Ile Lys
405 410 415Gly Gly Leu Met Val Asn Ser Leu Met Gly Asp Ser Asn Gly Ser Ile
420 425 430Pro Thr Pro Gln Pro Arg Thr Leu Arg Asn Thr Trp Gly Ala Phe Gly
435 440 445Gln Ala Val Ser Arg Ser Ser Ile Thr Phe Leu Ser Gln Asp Ala Ile
450 455 460Asp Ala Asn Val Pro Asp Leu Leu Asn Leu Arg Lys Gln Ile Arg Gly465 470 475 480Val Arg Gly Val Arg Asn Leu Thr Lys Arg Asp Met Lys Leu Asn Ala
485 490 495Glu Met Pro Asp Ile Arg Val Asp Pro Glu Thr Tyr Gln Val Phe Val
500 505 510Asn Gly Glu Leu Ile Thr Ser Lys Pro Ala Glu Thr Val Pro Met Ala
515 520 525Arg Arg Tyr Phe Leu Phe
530<210>17<211>423<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(400)<223>RXA02265<400>17tcaaaggaaa gcagtatatt gtcggttttt tagaggctct cccaaatatt gcgttggggg 60agctaaacta atttctgtta cctgacagaa aggggcaaaa ttg cat atc act cct 115
Leu His Ile Thr Pro
1 5cgt gaa caa gaa aaa ctg atg atc gtg gtg gcg gct gac ctt gca cgt 163Arg Glu Gln Glu Lys Leu Met Ile Val Val Ala Ala Asp Leu Ala Arg
10 15 20cgc cgt aaa gat cgc ggc cta aaa ctt aac cac cca gag gcc gtc gcc 211Arg Arg Lys Asp Arg Gly Leu Lys Leu Asn His Pro Glu Ala Val Ala
25 30 35ctc atc acg tat gaa ctg att gaa ggc gcc cgt gac gga cgc aca gtc 259Leu Ile Thr Tyr Glu Leu Ile Glu Gly Ala Arg Asp Gly Arg Thr Val
40 45 50gca gac ctt atg agc tgg gga agc acc att ttg act agg gat gat gtc 307Ala Asp Leu Met Ser Trp Gly Ser Thr Ile Leu Thr Arg Asp Asp Val
55 60 65tta gaa ggc atc cca gag atg atc cct gac atc cag gtt gaa gca acg 355Leu Glu Gly Ile Pro Glu Met Ile Pro Asp Ile Gln Val Glu Ala Thr70 75 80 85ttt gat gac ggc acc aag ctc gtc acc gtg cac aat ccc atc cga 400Phe Asp Asp Gly Thr Lys Leu Val Thr Val His Asn Pro Ile Arg
90 95 100taacccttga tgtttttagg agt 423<210>18<211>100<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>18Leu His Ile Thr Pro Arg Glu Gln Glu Lys Leu Met Ile Val Val Ala1 5 10 15Ala Asp Leu Ala Arg Arg Arg Lys Asp Arg Gly Leu Lys Leu Asn His
20 25 30Pro Glu Ala Val Ala Leu Ile Thr Tyr Glu Leu Ile Glu Gly Ala Arg
35 40 45Asp Gly Arg Thr Val Ala Asp Leu Met Ser Trp Gly Ser Thr Ile Leu
50 55 60Thr Arg Asp Asp Val Leu Glu Gly Ile Pro Glu Met Ile Pro Asp Ile65 70 75 80Gln Val Glu Ala Thr Phe Asp Asp Gly Thr Lys Leu Val Thr Val His
85 90 95Asn Pro Ile Arg
100<210>19<211>972<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(949)<223>RXA02278<400>19accattctgc ctgactaatc tgcgcaccga tgatggtttg gataaggtct tggaatggat 60ccgccatgag gtgatgatgc aggacttgca ggaagcctaa atg aca caa acc caa 115
Met Thr Gln Thr Gln
1 5cca gtg gga acc ctg cga ctg acc atc gat gat caa gga ccc caa ggt 163Pro Val Gly Thr Leu Arg Leu Thr Ile Asp Asp Gln Gly Pro Gln Gly
10 15 20caa agc cgt gcg gtg gag caa ttt cac cag ggt gcg ctt cga gtc atc 211Gln Ser Arg Ala Val Glu Gln Phe His Gln Gly Ala Leu Arg Val Ile
25 30 35cgg cca cac tac ttg gat gat tcc gga cag gtt tgc tac acc atc att 259Arg Pro His Tyr Leu Asp Asp Ser Gly Gln Val Cys Tyr Thr Ile Ile
40 45 50gcc att ggt ggc gga tac ctg ggc ggc gat gtg tat gag cag caa ttc 307Ala Ile Gly Gly Gly Tyr Leu Gly Gly Asp Val Tyr Glu Gln Gln Phe
55 60 65acg atc aaa gac aac gca aaa gct ttg atc acc acg caa tcg gcc acc 355Thr Ile Lys Asp Asn Ala Lys Ala Leu Ile Thr Thr Gln Ser Ala Thr70 75 80 85aag att tat cgc aca ccg caa gga cca gcc acg cag cac acc gaa atc 403Lys Ile Tyr Arg Thr Pro Gln Gly Pro Ala Thr Gln His Thr Glu Ile
90 95 100aac gtc ggt gaa aat gct gtg ctg gaa tac ttg gcg gat caa acc atc 451Asn Val Gly Glu Asn Ala Val Leu Glu Tyr Leu Ala Asp Gln Thr Ile
105 110 115gcg tac cgg gag gcc acc tat cat caa ttc acc aag gtg gcg ctg cac 499Ala Tyr Arg Glu Ala Thr Tyr His Gln Phe Thr Lys Val Ala Leu His
120 125 130ccg agc gca acg ttt gtg atg agc gaa caa atc acc cca ggc tgg cac 547Pro Ser Ala Thr Phe Val Met Ser Glu Gln Ile Thr Pro Gly Trp His
135 140 145ccc gac ggc aaa cac ttt gct tac gat gaa atg cgt cta cac acc gaa 595Pro Asp Gly Lys His Phe Ala Tyr Asp Glu Met Arg Leu His Thr Glu150 155 160 165atc acg gac tcc acc aca ggg cga ctc gtg ctc ttg gat aat tta ctg 643Ile Thr Asp Ser Thr Thr Gly Arg Leu Val Leu Leu Asp Asn Leu Leu
170 175 180ctc cgg ccg gac tcc cga gag gga agt ttt ggg tgg acg gaa cag tac 691Leu Arg Pro Asp Ser Arg Glu Gly Ser Phe Gly Trp Thr Glu Gln Tyr
185 190 195aca cat tca ggg cag atg att gtg atg ggg gaa ggc gtc gat aag cag 739Thr His Ser Gly Gln Met Ile Val Met Gly Glu Gly Val Asp Lys Gln
200 205 210ctt gtt gct gag ctg aat gag caa ctt gcc gcg cac cct gat gtg tac 787Leu Val Ala Glu Leu Asn Glu Gln Leu Ala Ala His Pro Asp Val Tyr
215 220 225ggc gcc gtc aat ttc tta agc gcg ccg ggc acg tta ctg cgc gga ttt 835Gly Ala Val Asn Phe Leu Ser Ala Pro Gly Thr Leu Leu Arg Gly Phe230 235 240 245att gcg cgc acg ctg agc aac cgc act gag gag ttg att aac ctg cac 883Ile Ala Arg Thr Leu Ser Asn Arg Thr Glu Glu Leu Ile Asn Leu His
250 255 260gaa cac att gcg tcg ctg ttg cgc ggg cgg tgg cgc ggg cag gaa ccg 931Glu His Ile Ala Ser Leu Leu Arg Gly Arg Trp Arg Gly Gln Glu Pro
265 270 275gtg aat ttg cgg aag tac tagacggcgt cgagaaatcg aag 972Val Asn Leu Arg Lys Tyr
280<210>20<211>283<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>20Met Thr Gln Thr Gln Pro Val Gly Thr Leu Arg Leu Thr Ile Asp Asp1 5 10 15Gln Gly Pro Gln Gly Gln Ser Arg Ala Val Glu Gln Phe His Gln Gly
20 25 30Ala Leu Arg Val Ile Arg Pro His Tyr Leu Asp Asp Ser Gly Gln Val
35 40 45Cys Tyr Thr Ile Ile Ala Ile Gly Gly Gly Tyr Leu Gly Gly Asp Val
50 55 60Tyr Glu Gln Gln Phe Thr Ile Lys Asp Asn Ala Lys Ala Leu Ile Thr65 70 75 80Thr Gln Ser Ala Thr Lys Ile Tyr Arg Thr Pro Gln Gly Pro Ala Thr
85 90 95Gln His Thr Glu Ile Asn Val Gly Glu Asn Ala Val Leu Glu Tyr Leu
100 105 110Ala Asp Gln Thr Ile Ala Tyr Arg Glu Ala Thr Tyr His Gln Phe Thr
115 120 125Lys Val Ala Leu His Pro Ser Ala Thr Phe Val Met Ser Glu Gln Ile
130 135 140Thr Pro Gly Trp His Pro Asp Gly Lys His Phe Ala Tyr Asp Glu Met145 150 155 160Arg Leu His Thr Glu Ile Thr Asp Ser Thr Thr Gly Arg Leu Val Leu
165 170 175Leu Asp Asn Leu Leu Leu Arg Pro Asp Ser Arg Glu Gly Ser Phe Gly
180 185 190Trp Thr Glu Gln Tyr Thr His Ser Gly Gln Met Ile Val Met Gly Glu
195 200 205Gly Val Asp Lys Gln Leu Val Ala Glu Leu Asn Glu Gln Leu Ala Ala
210 215 220His Pro Asp Val Tyr Gly Ala Val Asn Phe Leu Ser Ala Pro Gly Thr225 230 235 240Leu Leu Arg Gly Phe Ile Ala Arg Thr Leu Ser Asn Arg Thr Glu Glu
245 250 255Leu Ile Asn Leu His Glu His Ile Ala Ser Leu Leu Arg Gly Arg Trp
260 265 270Arg Gly Gln Glu Pro Val Asn Leu Arg Lys Tyr
275 280<210>21<211>594<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(571)<223>RXA02275<400>21gtttgtcaac ggtgagttga tcaccagcaa gccagcagag acagtgccaa tggcacgtcg 60ctacttcttg ttctaatccg ccaacaagga aggaagatcc atg att atc act gcg 115
Met Ile Ile Thr Ala
1 5atc gac acc aac atc tac gat gaa ccg gag ttt gtt gaa gga cgc gat 163Ile Asp Thr Asn Ile Tyr Asp Glu Pro Glu Phe Val Glu Gly Arg Asp
10 15 20gtc atc ggt gtg cgc ttt gaa gat tta gtt ttg gat aag cgc att caa 211Val Ile Gly Val Arg Phe Glu Asp Leu Val Leu Asp Lys Arg I1e Gln
25 30 35cgg gtt gca ctc ccc gga gga gaa gaa ctg ggg ttg cgg tta aac cac 259Arg Val Ala Leu Pro Gly Gly Glu Glu Leu Gly Leu Arg Leu Asn His
40 45 50ggg cat ccg att ctg cgt gaa ggt gat gtg ttg aaa gct gat gat aag 307Gly His Pro Ile Leu Arg Glu Gly Asp Val Leu Lys Ala Asp Asp Lys
55 60 65acg gta ttt gtg gtg gag att atc ccc acg gat gtt tta gtt atc acg 355Thr Val Phe Val Val Glu Ile Ile Pro Thr Asp Val Leu Val Ile Thr70 75 80 85cca agc gat att cac cag atg gga ttt gtg gcg cac tcc ctg gga aac 403Pro Ser Asp Ile His Gln Met Gly Phe Val Ala His Ser Leu Gly Asn
90 95 100agg cac ctg cca gca cag ttt tcc aag cca ggt gaa ttg aca gag aag 451Arg His Leu Pro Ala Gln Phe Ser Lys Pro Gly Glu Leu Thr Glu Lys
105 110 115gca gcc atg atc gtg caa tac gat cac acg gtg gtc agc ttc ttg gat 499Ala Ala Met Ile Val Gln Tyr Asp His Thr Val Val Ser Phe Leu Asp
120 125 130gag cac ggc atc gag tat cag cgc acc gaa ctt gtt ccg cca att cct 547Glu His Gly Ile Glu Tyr Gln Arg Thr Glu Leu Val Pro Pro Ile Pro
135 140 145ttc agg cat agc ggg cac aca cat tgatggatct tgacgctgat ttt 594Phe Arg His Ser Gly His Thr His150 155<210>22<211>157<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>22Met Ile Ile Thr Ala Ile Asp Thr Asn Ile Tyr Asp Glu Pro Glu Phe1 5 10 15Val Glu Gly Arg Asp Val Ile Gly Val Arg Phe Glu Asp Leu Val Leu
20 25 30Asp Lys Arg Ile Gln Arg Val Ala Leu Pro Gly Gly Glu Glu Leu Gly
35 40 45Leu Arg Leu Asn His Gly His Pro Ile Leu Arg Glu Gly Asp Val Leu
50 55 60Lys Ala Asp Asp Lys Thr Val Phe Val Val Glu Ile Ile Pro Thr Asp65 70 75 80Val Leu Val Ile Thr Pro Ser Asp Ile His Gln Met Gly Phe Val Ala
85 90 95His Ser Leu Gly Asn Arg His Leu Pro Ala Gln Phe Ser Lys Pro Gly
100 105 110Glu Leu Thr Glu Lys Ala Ala Met Ile Val Gln Tyr Asp His Thr Val
115 120 125Val Ser Phe Leu Asp Glu His Gly Ile Glu Tyr Gln Arg Thr Glu Leu
130 135 140Val Pro Pro Ile Pro Phe Arg His Ser Gly His Thr His145 150 155<210>23<211>801<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(778)<223>RXA02276<400>23atcacacggt ggtcagcttc ttggatgagc acggcatcga gtatcagcgc accgaacttg 60ttccgccaat tcctttcagg catagcgggc acacacattg atg gat ctt gac gct 115
Met Asp Leu Asp Ala
1 5gat ttt ctg ctg ttg cat tta tcg gat tca gca ctt cca acg gga gcg 163Asp Phe Leu Leu Leu His Leu Ser Asp Ser Ala Leu Pro Thr Gly Ala
10 15 20ttt gcg cac tca ttt gga ttt gaa act tat atg gat gca gag cga atc 211Phe Ala His Ser Phe Gly Phe Glu Thr Tyr Met Asp Ala Glu Arg Ile
25 30 35acc aat gca gag gag ttc caa gac tgg ctg aaa gtc ctg ctt aag gtg 259Thr Asn Ala Glu Glu Phe Gln Asp Trp Leu Lys Val Leu Leu Lys Val
40 45 50caa ttg acc agc tct gat gct ttg gca atg agg atg ttt tac gcc acc 307Gln Leu Thr Ser Ser Asp Ala Leu Ala Met Arg Met Phe Tyr Ala Thr
55 60 65ccg acg gtg tct gag ctg aaa cgg ctg gat gag cgc ctt ttt gct gga 355Pro Thr Val Ser Glu Leu Lys Arg Leu Asp Glu Arg Leu Phe Ala Gly70 75 80 85act ccg gcg aga gaa att cgg gaa gct aat gct cga atg ggt acg cgc 403Thr Pro Ala Arg Glu Ile Arg Glu Ala Asn Ala Arg Met Gly Thr Arg
90 95 100atg gca gag atc gtg gct gaa acc tac tcc gtg ccc ctg att gtt gag 451Met Ala Glu Ile Val Ala Glu Thr Tyr Ser Val Pro Leu Ile Val Glu
105 110 115tat ctc gaa ttg att caa cat cga gag cta tca ggg cac ccg gct ttg 499Tyr Leu Glu Leu Ile Gln His Arg Glu Leu Ser Gly His Pro Ala Leu
120 125 130gct ttg gct ctt gcc acc cac agc gcg ggg att gat gtg gat cga gca 547Ala Leu Ala Leu Ala Thr His Ser Ala Gly Ile Asp Val Asp Arg Ala
135 140 145atc cac gct cac ctc acg gca acg gtg agt tcg ctg atc caa aat gcg 595Ile His Ala His Leu Thr Ala Thr Val Ser Ser Leu Ile Gln Asn Ala150 155 160 165gtt cgt ggc atc cca ctg ggg caa atg gca ggt cag cgg gtg atg ttc 643Val Arg Gly Ile Pro Leu Gly Gln Met Ala Gly Gln Arg Val Met Phe
170 175 180gcc atg cgt gag cat atc ggt gcg gcc gtg aaa cgt agc gcg aac ttg 691Ala Met Arg Glu His Ile Gly Ala Ala Val Lys Arg Ser Ala Asn Leu
185 190 195gat gag att gat ttc tgt tcg ggt gat cca ggc ttg gat att tca caa 739Asp Glu Ile Asp Phe Cys Ser Gly Asp Pro Gly Leu Asp Ile Ser Gln
200 205 210atg gtt cat gaa acc caa cgc gca cga cta ttt atg agt taagaaggag 788Met Val His Glu Thr Gln Arg Ala Arg Leu Phe Met Ser
215 220 225aaaagaaaca tgg 801<210>24<211>226<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>24Met Asp Leu Asp Ala Asp Phe Leu Leu Leu His Leu Ser Asp Ser Ala1 5 10 15Leu Pro Thr Gly Ala Phe Ala His Ser Phe Gly Phe Glu Thr Tyr Met
20 25 30Asp Ala Glu Arg Ile Thr Asn Ala Glu Glu Phe Gln Asp Trp Leu Lys
35 40 45Val Leu Leu Lys Val Gln Leu Thr Ser Ser Asp Ala Leu Ala Met Arg
50 55 60Met Phe Tyr Ala Thr Pro Thr Val Ser Glu Leu Lys Arg Leu Asp Glu65 70 75 80Arg Leu Phe Ala Gly Thr Pro Ala Arg Glu Ile Arg Glu Ala Asn Ala
85 90 95Arg Met Gly Thr Arg Met Ala Glu Ile Val Ala Glu Thr Tyr Ser Val
100 105 110Pro Leu Ile Val Glu Tyr Leu Glu Leu Ile Gln His Arg Glu Leu Ser
115 120 125Gly His Pro Ala Leu Ala Leu Ala Leu Ala Thr His Ser Ala Gly Ile
130 135 140Asp Val Asp Arg Ala Ile His Ala His Leu Thr Ala Thr Val Ser Ser145 150 155 160Leu Ile Gln Asn Ala Val Arg Gly Ile Pro Leu Gly Gln Met Ala Gly
165 170 175Gln Arg Val Met Phe Ala Met Arg Glu His Ile Gly Ala Ala Val Lys
180 185 190Arg Ser Ala Asn Leu Asp Glu Ile Asp Phe Cys Ser Gly Asp Pro Gly
195 200 205Leu Asp Ile Ser Gln Met Val His Glu Thr Gln Arg Ala Arg Leu Phe
210 215 220Met Ser225<210>25<211>738<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(715)<223>RXA02277<400>25attgatttct gttcgggtga tccaggcttg gatatttcac aaatggttca tgaaacccaa 60cgcgcacgac tatttatgag ttaagaagga gaaaagaaac atg ggt cca atc aga 115
Met Gly Pro Ile Arg
1 5atc ggc gta ggc ggg ccg gtc ggc gcc gga aaa acg cag ctg gta gag 163Ile Gly Val Gly Gly Pro Val Gly Ala Gly Lys Thr Gln Leu Val Glu
10 15 20cgg att acg cga gcg ctt atc gac gaa gtc agc atg gct gca atc act 211Arg Ile Thr Arg Ala Leu Ile Asp Glu Val Ser Met Ala Ala Ile Thr
25 30 35aac gat atc tac acc att gaa gac gcc aag att ctt gcc gcc aat gga 259Asn Asp Ile Tyr Thr Ile Glu Asp Ala Lys Ile Leu Ala Ala Asn Gly
40 45 50gtg ctg cca gaa gaa cgc att gtt ggc att gaa act gga gga tgc cca 307Val Leu Pro Glu Glu Arg Ile Val Gly Ile Glu Thr Gly Gly Cys Pro
55 60 65cac act gcg att cgt gaa gac acc tcc atg aat gat gca gcg atc aaa 355His Thr Ala Ile Arg Glu Asp Thr Ser Met Asn Asp Ala Ala Ile Lys70 75 80 85gac ctt gtg gaa cgc ttc cca gat ctg gaa ctc atc ttt gtg gaa tct 403Asp Leu Val Glu Arg Phe Pro Asp Leu Glu Leu Ile Phe Val Glu Ser
90 95 100ggt gga gat aat ctc tct gca acg ttc tcg cca gag ctg gtg gat ttt 451Gly Gly Asp Asn Leu Ser Ala Thr Phe Ser Pro Glu Leu Val Asp Phe
105 110 115tcc atc tac atc atc gat gtt gcc caa ggt gag aag atc ccg agg aaa 499Ser Ile Tyr Ile Ile Asp Val Ala Gln Gly Glu Lys Ile Pro Arg Lys
120 125 130gct ggc caa ggc atg att aag tcg gat ttg ttt atc att aat aaa act 547Ala Gly Gln Gly Met Ile Lys Ser Asp Leu Phe Ile Ile Asn Lys Thr
135 140 145gac ctt gcc cca tat gtt ggt gcc aac cta gat gtc atg gtg gaa gat 595Asp Leu Ala Pro Tyr Val Gly Ala Asn Leu Asp Val Met Val Glu Asp150 155 160 165gcc aaa gca ttc cgc aag aac aaa cca ttc tgc ctg act aat ctg cgc 643Ala Lys Ala Phe Arg Lys Asn Lys Pro Phe Cys Leu Thr Asn Leu Arg
170 175 180acc gat gat ggt ttg gat aag gtc ttg gaa tgg atc cgc cat gag gtg 691Thr Asp Asp Gly Leu Asp Lys Val Leu Glu Trp Ile Arg His Glu Val
185 190 195atg atg cag gac ttg cag gaa gcc taaatgacac aaacccaacc agt 738Met Met Gln Asp Leu Gln Glu Ala
200 205<210>26<211>205<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>26Met Gly Pro Ile Arg Ile Gly Val Gly Gly Pro Val Gly Ala Gly Lys1 5 10 15Thr Gln Leu Val Glu Arg Ile Thr Arg Ala Leu Ile Asp Glu Val Ser
20 25 30Met Ala Ala Ile Thr Asn Asp Ile Tyr Thr Ile Glu Asp Ala Lys Ile
35 40 45Leu Ala Ala Asn Gly Val Leu Pro Glu Glu Arg Ile Val Gly Ile Glu
50 55 60Thr Gly Gly Cys Pro His Thr Ala Ile Arg Glu Asp Thr Ser Met Asn65 70 75 80Asp Ala Ala Ile Lys Asp Leu Val Glu Arg Phe Pro Asp Leu Glu Leu
85 90 95Ile Phe Val Glu Ser Gly Gly Asp Asn Leu Ser Ala Thr Phe Ser Pro
100 105 110Glu Leu Val Asp Phe Ser Ile Tyr Ile Ile Asp Val Ala Gln Gly Glu
115 120 125Lys Ile Pro Arg Lys Ala Gly Gln Gly Met Ile Lys Ser Asp Leu Phe
130 135 140Ile Ile Asn Lys Thr Asp Leu Ala Pro Tyr Val Gly Ala Asn Leu Asp145 150 155 160Val Met Val Glu Asp Ala Lys Ala Phe Arg Lys Asn Lys Pro Phe Cys
165 170 175Leu Thr Asn Leu Arg Thr Asp Asp Gly Leu Asp Lys Val Leu Glu Trp
180 185 190Ile Arg His Glu Val Met Met Gln Asp Leu Gln Glu Ala
195 200 205<210>27<211>1119<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1096)<223>RXA02603<400>27gcatggtgct tggcgtgcat tacccaaccg atgtgctagc cggcgcgttg ttgggagcag 60cgaccgcaga ggccgtccat aagatcgaaa gggctacgaa gtg agc gaa cac gcc 115
Val Ser Glu His Ala
1 5gct gaa cat cac cgc gat acc caa aat ttc tta acc tcc gaa ccg cac 163Ala Glu His His Arg Asp Thr Gln Asn Phe Leu Thr Ser Glu Pro His
10 15 20acc acg gca atc gaa gac aac aag aag cgc caa ccg ccg aaa aac ctt 211Thr Thr Ala Ile Glu Asp Asn Lys Lys Arg Gln Pro Pro Lys Asn Leu
25 30 35gct gac ggc atg atc aag gcg ctg cgc ccc aag cag tgg gtc aag aac 259Ala Asp Gly Met Ile Lys Ala Leu Arg Pro Lys Gln Trp Val Lys Asn
40 45 50gtt ctt gtg cta gca gca cca ctt gct gct ggt gca gat gcg atc ttc 307Val Leu Val Leu Ala Ala Pro Leu Ala Ala Gly Ala Asp Ala Ile Phe
55 60 65aac cag cgc acg atc atc gac gtt gct atc gca ttc gta gtg ttc tgc 355Asn Gln Arg Thr Ile Ile Asp Val Ala Ile Ala Phe Val Val Phe Cys70 75 80 85ttc ggt gca tca gcc att tac ttg gtt aat gat gcc cgt gac gtg gaa 403Phe Gly Ala Ser Ala Ile Tyr Leu Val Asn Asp Ala Arg Asp Val Glu
90 95 100gct gac cgc gag cac cca acc aag cgt ttc cgc ccc atc gct gca gga 451Ala Asp Arg Glu His Pro Thr Lys Arg Phe Arg Pro Ile Ala Ala Gly
105 110 115gtc ctg cca gta gga atg gca tac ggc atg gcc gtg gcg ctc att gca 499Val Leu Pro Val Gly Met Ala Tyr Gly Met Ala Val Ala Leu Ile Ala
120 125 130cta tcc atc gga ctg tct ttc ctc gcc acc gac ggc gtg gca ctt gcc 547Leu Ser Ile Gly Leu Ser Phe Leu Ala Thr Asp Gly Val Ala Leu Ala
135 140 145tgc gtg att ggc gtg tac att gcg ctg cag ctg gga tac tgc ttc ggt 595Cys Val Ile Gly Val Tyr Ile Ala Leu Gln Leu Gly Tyr Cys Phe Gly150 155 160 165tgg aag cac atg cca gtg atc gat att gcg ctt gtc tcc tcc gga ttc 643Trp Lys His Met Pro Val Ile Asp Ile Ala Leu Val Ser Ser Gly Phe
170 175 180atg ctc cgc gca atg gca ggt ggt gtc gca gca ggc atc gag cta tcc 691Met Leu Arg Ala Met Ala Gly Gly Val Ala Ala Gly Ile Glu Leu Ser
185 190 195cag tgg ttc ctg cta gtc gct gcg ttt ggt tcc ctg ttc atg gca tct 739Gln Trp Phe Leu Leu Val Ala Ala Phe Gly Ser Leu Phe Met Ala Ser
200 205 210gga aag cgc tac gca gaa atc ctt ctg cac gag cgc acc ggc gct aag 787Gly Lys Arg Tyr Ala Glu Ile Leu Leu His Glu Arg Thr Gly Ala Lys
215 220 225atc cgc aag tcc ctg gaa agc tac acc ccc acc tac ctg cgc ttc gtt 835Ile Arg Lys Ser Leu Glu Ser Tyr Thr Pro Thr Tyr Leu Arg Phe Val230 235 240 245tgg acc atg gca gca aca gca gtg gtc atg tcc tac gca ctg tgg ggc 883Trp Thr Met Ala Ala Thr Ala Val Val Met Ser Tyr Ala Leu Trp Gly
250 255 260ttc gac ctt tcc caa cac tcc acc gac gca ggt ccg tgg tac caa atc 931Phe Asp Leu Ser Gln His Ser Thr Asp Ala Gly Pro Trp Tyr Gln Ile
265 270 275tcc atg gtt cca ttc acc atc gcc atc ctg cgc tac gca gcc ggc gta 979Ser Met Val Pro Phe Thr Ile Ala Ile Leu Arg Tyr Ala Ala Gly Val
280 285 290gac acc ggc gac ggc ggt gcc cct gac gaa gtg gca ctc agc gac aaa 1027Asp Thr Gly Asp Gly Gly Ala Pro Asp Glu Val Ala Leu Ser Asp Lys
295 300 305gtt ctg cag gta cta gcc cta gca tgg gtt ttc tgc atc gtg atg gct 1075Val Leu Gln Val Leu Ala Leu Ala Trp Val Phe Cys Ile Val Met Ala310 315 320 325gtg tac atc atg ccg atg ttt tgaatattta ccaatgaaca tgc 1119Val Tyr Ile Met Pro Met Phe
330<210>28<211>332<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>28Val Ser Glu His Ala Ala Glu His His Arg Asp Thr Gln Asn Phe Leu1 5 10 15Thr Ser Glu Pro His Thr Thr Ala Ile Glu Asp Asn Lys Lys Arg Gln
20 25 30Pro Pro Lys Asn Leu Ala Asp Gly Met Ile Lys Ala Leu Arg Pro Lys
35 40 45Gln Trp Val Lys Asn Val Leu Val Leu Ala Ala Pro Leu Ala Ala Gly
50 55 60Ala Asp Ala Ile Phe Asn Gln Arg Thr Ile Ile Asp Val Ala Ile Ala65 70 75 80Phe Val Val Phe Cys Phe Gly Ala Ser Ala Ile Tyr Leu Val Asn Asp
85 90 95Ala Arg Asp Val Glu Ala Asp Arg Glu His Pro Thr Lys Arg Phe Arg
100 105 110Pro Ile Ala Ala Gly Val Leu Pro Val Gly Met Ala Tyr Gly Met Ala
115 120 125Val Ala Leu Ile Ala Leu Ser Ile Gly Leu Ser Phe Leu Ala Thr Asp
130 135 140Gly Val Ala Leu Ala Cys Val Ile Gly Val Tyr Ile Ala Leu Gln Leu145 150 155 160Gly Tyr Cys Phe Gly Trp Lys His Met Pro Val Ile Asp Ile Ala Leu
165 170 175Val Ser Ser Gly Phe Met Leu Arg Ala Met Ala Gly Gly Val Ala Ala
180 185 190Gly Ile Glu Leu Ser Gln Trp Phe Leu Leu Val Ala Ala Phe Gly Ser
195 200 205Leu Phe Met Ala Ser Gly Lys Arg Tyr Ala Glu Ile Leu Leu His Glu
210 215 220Arg Thr Gly Ala Lys Ile Arg Lys Ser Leu Glu Ser Tyr Thr Pro Thr225 230 235 240Tyr Leu Arg Phe Val Trp Thr Met Ala Ala Thr Ala Val Val Met Ser
245 250 255Tyr Ala Leu Trp Gly Phe Asp Leu Ser Gln His Ser Thr Asp Ala Gly
260 265 270Pro Trp Tyr Gln Ile Ser Met Val Pro Phe Thr Ile Ala Ile Leu Arg
275 280 285Tyr Ala Ala Gly Val Asp Thr Gly Asp Gly Gly Ala Pro Asp Glu Val
290 295 300Ala Leu Ser Asp Lys Val Leu Gln Val Leu Ala Leu Ala Trp Val Phe305 310 315 320Cys Ile Val Met Ala Val Tyr Ile Met Pro Met Phe
325 330<210>29<211>2004<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1981)<223>RXA01385<400>29atcacacttt cattcgcagt gtgatctgaa ctacatttct ggttactgta cggaacacgc 60tccgtgaatg agataggaaa tcccctcgaa aggaccagac atg cag ttt cat tat 115
Met Gln Phe His Tyr
1 5gaa gga tac gca acc ggt gac cca atg gag atg cgc gcg gaa ggt agc 163Glu Gly Tyr Ala Thr Gly Asp Pro Met Glu Met Arg Ala Glu Gly Ser
10 15 20gga atc aac cgc ccg gac gat ctc ccc gag gtc atg gat gtt ctc atc 211Gly Ile Asn Arg Pro Asp Asp Leu Pro Glu Val Met Asp Val Leu Ile
25 30 35gtt ggt gca ggt ccg gct ggc acc atc gca gcg gct cag ctt tcc cga 259Val Gly Ala Gly Pro Ala Gly Thr Ile Ala Ala Ala Gln Leu Ser Arg
40 45 50ttc ccc aat gtg acc acc cgc ctc gta gag aga agc gac cgt cgc ctc 307Phe Pro Asn Val Thr Thr Arg Leu Val Glu Arg Ser Asp Arg Arg Leu
55 60 65gaa cta gcc aat gca gat ggc gtg cac tcc cga acc att gaa act ttc 355Glu Leu Ala Asn Ala Asp Gly Val His Ser Arg Thr Ile Glu Thr Phe70 75 80 85cag gca ttt ggt ttc gcc cac gag atc ctc gcc gaa gct cat gaa atc 403Gln Ala Phe Gly Phe Ala His Glu Ile Leu Ala Glu Ala His Glu Ile
90 95 100acc gac atg gcg ttc tgg aag ccg gac ccg caa aac cct cgt gag atc 451Thr Asp Met Ala Phe Trp Lys Pro Asp Pro Gln Asn Pro Arg Glu Ile
105 110 115att cgc gac aac agc acc cgc gag ctg cca cag cac atc agt gaa ttt 499Ile Arg Asp Asn Ser Thr Arg Glu Leu Pro Gln His Ile Ser Glu Phe
120 125 130ccg atg gcg ttg ctc acc cag acc cgc atc atc gac cac ttc aac cgg 547Pro Met Ala Leu Leu Thr Gln Thr Arg Ile Ile Asp His Phe Asn Arg
135 140 145ttc atg aag aac tcc cca acc agg atg aag cct gac tat gga tac gag 595Phe Met Lys Asn Ser Pro Thr Arg Met Lys Pro Asp Tyr Gly Tyr Glu150 155 160 165ttc gtg gac ttt gaa gta gaa gaa gac gca gaa tat ccg gta att gtc 643Phe Val Asp Phe Glu Val Glu Glu Asp Ala Glu Tyr Pro Val Ile Val
170 175 180acc ctc cgc cgc acc agt ggc gag caa act ggc gaa ttg gtc acc gtc 691Thr Leu Arg Arg Thr Ser Gly Glu Gln Thr Gly Glu Leu Val Thr Val
185 190 195cga acc aag tac ctg gtc ggt gcc gat ggt gca cga agc caa gtg cgc 739Arg Thr Lys Tyr Leu Val Gly Ala Asp Gly Ala Arg Ser Gln Val Arg
200 205 210aaa tca ctg gga tac cga ctc caa ggt aag cag gct aac cac gct tgg 787Lys Ser Leu Gly Tyr Arg Leu Gln Gly Lys Gln Ala Asn His Ala Trp
215 220 225ggt gtg atg gat att cac gcg aac acc gag ttc ccc gac gtg cgc aag 835Gly Val Met Asp Ile His Ala Asn Thr Glu Phe Pro Asp Val Arg Lys230 235 240 245aag tgc acc atc aaa tct gat tcg ggt cgc acc atc ttg ctc atc cca 883Lys Cys Thr Ile Lys Ser Asp Ser Gly Arg Thr Ile Leu Leu Ile Pro
250 255 260cgt gag ggt ggc ttc ctc ttc cgt ctc tac gtt gac ctg ggc gaa gta 931Arg Glu Gly Gly Phe Leu Phe Arg Leu Tyr Val Asp Leu Gly Glu Val
265 270 275cct gat gat ggc agc aag gct gtt cgt gat acc cca ctc cag gat gtc 979Pro Asp Asp Gly Ser Lys Ala Val Arg Asp Thr Pro Leu Gln Asp Val
280 285 290atc gac acc gcg aac cag atc atg gct cca ttc acc ctc gac gtg aaa 1027Ile Asp Thr Ala Asn Gln Ile Met Ala Pro Phe Thr Leu Asp Val Lys
295 300 305aac gtt gtg tgg aac tcc atc tac gag gta ggc cac cgc gtc gca gac 1075Asn Val Val Trp Asn Ser Ile Tyr Glu Val Gly His Arg Val Ala Asp310 315 320 325cat ttc gat gac cgt gtt tca gaa aaa acc tcg agc gaa cac cca cgc 1123His Phe Asp Asp Arg Val Ser Glu Lys Thr Ser Ser Glu His Pro Arg
330 335 340att ttc att gct ggc gac gcc tgc cac acc cac agc gct aag gct ggc 1171Ile Phe Ile Ala Gly Asp Ala Cys His Thr His Ser Ala Lys Ala Gly
345 350 355cag ggc atg aac gtg tcc atg cag gac gga ttc aac ctt ggc tgg aag 1219Gln Gly Met Asn Val Ser Met Gln Asp Gly Phe Asn Leu Gly Trp Lys
360 365 370ctt gga cat gtg gcc agc gga aat agc cca cgc gaa cta ctt cag acc 1267Leu Gly His Val Ala Ser Gly Asn Ser Pro Arg Glu Leu Leu Gln Thr
375 380 385tac gct gaa gag cgc gaa gac att gcc tac aag ctc atc gag tac gac 1315Tyr Ala Glu Glu Arg Glu Asp Ile Ala Tyr Lys Leu Ile Glu Tyr Asp390 395 400 405aag aac tgg tca aca ctc atg gca aag cca agc agc gaa atg ggc agt 1363Lys Asn Trp Ser Thr Leu Met Ala Lys Pro Ser Ser Glu Met Gly Ser
410 415 420gcc caa gac ctt gag gat ttc tac cgc gcg aac tct gag ttc aat gcc 1411Ala Gln Asp Leu Glu Asp Phe Tyr Arg Ala Asn Ser Glu Phe Asn Ala
425 430 435ggc tac atg acc cac tat cct cct tct tcc atc aca atg gat ggc agc 1459Gly Tyr Met Thr His Tyr Pro Pro Ser Ser Ile Thr Met Asp Gly Ser
440 445 450aac caa gat ctg gca aag ggc tac cca att ggc cga cgc ttc aag tca 1507Asn Gln Asp Leu Ala Lys Gly Tyr Pro Ile Gly Arg Arg Phe Lys Ser
455 460 465gcg atg gtt ggt cga gtc tgc gac ttc acc gaa aca cac ctc ggt cac 1555Ala Met Val Gly Arg Val Cys Asp Phe Thr Glu Thr His Leu Gly His470 475 480 485caa gca aca gcc gac gga cgc atg cgc gca tac gtc ttc gca gga tcc 1603Gln Ala Thr Ala Asp Gly Arg Met Arg Ala Tyr Val Phe Ala Gly Ser
490 495 500gat gca ctt aac ggc gag ggt tct gag cta gac cgc tgg gca gaa tgg 1651Asp Ala Leu Asn Gly Glu Gly Ser Glu Leu Asp Arg Trp Ala Glu Trp
505 510 515gca gag gcg aac ctt gac ccc acg ctt gtc gac gcc aag gtg att tac 1699Ala Glu Ala Asn Leu Asp Pro Thr Leu Val Asp Ala Lys Val Ile Tyr
520 525 530caa agc cct tat acc gag ctc gac acc cgc cag gtt cca tcc gtg ttc 1747Gln Ser Pro Tyr Thr Glu Leu Asp Thr Arg Gln Val Pro Ser Val Phe
535 540 545aaa cct gca gtc ggg atc ttc gaa ctg acc aat gtg gaa aac tcc ttc 1795Lys Pro Ala Val Gly Ile Phe Glu Leu Thr Asn Val Glu Asn Ser Phe550 555 560 565ggt atc acc acg gac tcc gac atc ttt gat agt cgc gag atc tcc cgc 1843Gly Ile Thr Thr Asp Ser Asp Ile Phe Asp Ser Arg Glu Ile Ser Arg
570 575 580gat ggt gtc gtg gtg gta gtc cga cca gac caa tac gtt tcc gga atc 1891Asp Gly Val Val Val Val Val Arg Pro Asp Gln Tyr Val Ser Gly Ile
585 590 595ttc cca ctc act gat acc caa ggg ctt ggc gaa ttc ctc acc gga tac 1939Phe Pro Leu Thr Asp Thr Gln Gly Leu Gly Glu Phe Leu Thr Gly Tyr
600 605 610ttc ccc aaa atg aaa ggc gca cat cag cta atc aac gcg aac 1981Phe Pro Lys Met Lys Gly Ala His Gln Leu Ile Asn Ala Asn
615 620 625taaggcacag ctgttaaaac agt 2004<210>30<211>627<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>30Met Gln Phe His Tyr Glu Gly Tyr Ala Thr Gly Asp Pro Met Glu Met1 5 10 15Arg Ala Glu Gly Ser Gly Ile Asn Arg Pro Asp Asp Leu Pro Glu Val
20 25 30Met Asp Val Leu Ile Val Gly Ala Gly Pro Ala Gly Thr Ile Ala Ala
35 40 45Ala Gln Leu Ser Arg Phe Pro Asn Val Thr Thr Arg Leu Val Glu Arg
50 55 60Ser Asp Arg Arg Leu Glu Leu Ala Asn Ala Asp Gly Val His Ser Arg65 70 75 80Thr Ile Glu Thr Phe Gln Ala Phe Gly Phe Ala His Glu Ile Leu Ala
85 90 95Glu Ala His Glu Ile Thr Asp Met Ala Phe TTp Lys Pro Asp Pro Gln
100 105 110Asn Pro Arg Glu Ile Ile Arg Asp Asn Ser Thr Arg Glu Leu Pro Gln
115 120 125His Ile Ser Glu Phe Pro Met Ala Leu Leu Thr Gln Thr Arg Ile Ile
130 135 140Asp His Phe Asn Arg Phe Met Lys Asn Ser Pro Thr Arg Met Lys Pro145 150 155 160Asp Tyr Gly Tyr Glu Phe Val Asp Phe Glu Val Glu Glu Asp Ala Glu
165 170 175Tyr Pro Val Ile Val Thr Leu Arg Arg Thr Ser Gly Glu Gln Thr Gly
180 185 190Glu Leu Val Thr Val Arg Thr Lys Tyr Leu Val Gly Ala Asp Gly Ala
195 200 205Arg Ser Gln Val Arg Lys Ser Leu Gly Tyr Arg Leu Gln Gly Lys Gln
210 215 220Ala Asn His Ala Trp Gly Val Met Asp Ile His Ala Asn Thr Glu Phe225 230 235 240Pro Asp Val Arg Lys Lys Cys Thr Ile Lys Ser Asp Ser Gly Arg Thr
245 250 255Ile Leu Leu Ile Pro Arg Glu Gly Gly Phe Leu Phe Arg Leu Tyr Val
260 265 270Asp Leu Gly Glu Val Pro Asp Asp Gly Ser Lys Ala Val Arg Asp Thr
275 280 285Pro Leu Gln Asp Val Ile Asp Thr Ala Asn Gln Ile Met Ala Pro Phe
290 295 300Thr Leu Asp Val Lys Asn Val Val Trp Asn Ser Ile Tyr Glu Val Gly305 310 315 320His Arg Val Ala Asp His Phe Asp Asp Arg Val Ser Glu Lys Thr Ser
325 330 335Ser Glu His Pro Arg Ile Phe Ile Ala Gly Asp Ala Cys His Thr His
340 345 350Ser Ala Lys Ala Gly Gln Gly Met Asn Val Ser Met Gln Asp Gly Phe
355 360 365Asn Leu Gly Trp Lys Leu Gly His Val Ala Ser Gly Asn Ser Pro Arg
370 375 380Glu Leu Leu Gln Thr Tyr Ala Glu Glu Arg Glu Asp Ile Ala Tyr Lys385 390 395 400Leu Ile Glu Tyr Asp Lys Asn Trp Ser Thr Leu Met Ala Lys Pro Ser
405 410 415Ser Glu Met Gly Ser Ala Gln Asp Leu Glu Asp Phe Tyr Arg Ala Asn
420 425 430Ser Glu Phe Asn Ala Gly Tyr Met Thr His Tyr Pro Pro Ser Ser Ile
435 440 445Thr Met Asp Gly Ser Asn Gln Asp Leu Ala Lys Gly Tyr Pro Ile Gly
450 455 460Arg Arg Phe Lys Ser Ala Met Val Gly Arg Val Cys Asp Phe Thr Glu465 470 475 480Thr His Leu Gly His Gln Ala Thr Ala Asp Gly Arg Met Arg Ala Tyr
485 490 495Val Phe Ala Gly Ser Asp Ala Leu Asn Gly Glu Gly Ser Glu Leu Asp
500 505 510Arg Trp Ala Glu Trp Ala Glu Ala Asn Leu Asp Pro Thr Leu Val Asp
515 520 525Ala Lys Val Ile Tyr Gln Ser Pro Tyr Thr Glu Leu Asp Thr Arg Gln
530 535 540Val Pro Ser Val Phe Lys Pro Ala Val Gly Ile Phe Glu Leu Thr Asn545 550 555 560Val Glu Asn Ser Phe Gly Ile Thr Thr Asp Ser Asp Ile Phe Asp Ser
565 570 575Arg Glu Ile Ser Arg Asp Gly Val Val Val Val Val Arg Pro Asp Gln
580 585 590Tyr Val Ser Gly Ile Phe Pro Leu Thr Asp Thr Gln Gly Leu Gly Glu
595 600 605Phe Leu Thr Gly Tyr Phe Pro Lys Met Lys Gly Ala His Gln Leu Ile
610 615 620Asn Ala Asn625<210>31<211>915<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(892)<223>RXN00675<400>31gtggaagtac tcacctcaag gtggccggaa ccgcatgcat tgtgcggtgc cggccacctt 60tggttttaac agtttaattt gaagaaagag acgtggaagc atg ggt ttc cgt tcc 115
Met Gly Phe Arg Ser
1 5aag aag aag gtt att gcg gca aag acc gcc gct gag ctg gac gcg atg 163Lys Lys Lys Val Ile Ala Ala Lys Thr Ala Ala Glu Leu Asp Ala Met
10 15 20cag gcg gcg ggt gag atc gtc ggc aag gct ttg cag gct gtg cgc gct 211Gln Ala Ala Gly Glu Ile Val Gly Lys Ala Leu Gln Ala Val Arg Ala
25 30 35gag gct aaa gct ggc atg agc acg tgg gat ctg gat cag atc gcg gag 259Glu Ala Lys Ala Gly Met Ser Thr Trp Asp Leu Asp Gln Ile Ala Glu
40 45 50cag gtt atc cgc gat gct ggc gcc gtt cct aca ttc ctg ggt tac cag 307Gln Val Ile Arg Asp Ala Gly Ala Val Pro Thr Phe Leu Gly Tyr Gln
55 60 65ggt ttt ccg gca tca gtg tgc gct tcg gtc aat gag gtg att gtt cac 355Gly Phe Pro Ala Ser Val Cys Ala Ser Val Asn Glu Val Ile Val His70 75 80 85ggc att cca tcc aag gag acc atc ttg gag gaa ggc gat ctg gtg tcc 403Gly Ile Pro Ser Lys Glu Thr Ile Leu Glu Glu Gly Asp Leu Val Ser
90 95 100atc gac tgc ggc gca acc ttt gat ggt tgg gtc ggc gat tcc gcg tgg 451Ile Asp Cys Gly Ala Thr Phe Asp Gly Trp Val Gly Asp Ser Ala Trp
105 110 115agc ttc ggc atc ggc gag ctg gac gag gac gtc cag ggt ctc aac ttg 499Ser Phe Gly Ile Gly Glu Leu Asp Glu Asp Val Gln Gly Leu Asn Leu
120 125 130gct acc gag tgg gtc ctc atg gaa ggc atg aag gcc atg gtt cca ggc 547Ala Thr Glu Trp Val Leu Met Glu Gly Met Lys Ala Met Val Pro Gly
135 140 145aac cgt ttg acc gat gtc tcc cac gct ctc gag gtc gca acc cgc aag 595Asn Arg Leu Thr Asp Val Ser His Ala Leu Glu Val Ala Thr Arg Lys150 155 160 165gct gag tcc aag ttc ggc gtc gcg ctc ggc atc gtc gat ggc tac ggc 643Ala Glu Ser Lys Phe Gly Val Ala Leu Gly Ile Val Asp Gly Tyr Gly
170 175 180gga cac ggc att ggc cgc cac atg cac gag gag cca tac ttg gct aat 691Gly His Gly Ile Gly Arg His Met His Glu Glu Pro Tyr Leu Ala Asn
185 190 195gag ggc aag gcc ggc aag ggc cct gtg att cag gag ggc tcc gtg ctc 739Glu Gly Lys Ala Gly Lys Gly Pro Val Ile Gln Glu Gly Ser Val Leu
200 205 210gcc att gag cct atg ctc acc ctc ggc acc gaa gat tcc gca gtg ctg 787Ala Ile Glu Pro Met Leu Thr Leu Gly Thr Glu Asp Ser Ala Val Leu
215 220 225gaa gat gat tgg act gtc gtg act ctc gac ggt tca tgg gca tca cac 835Glu Asp Asp Trp Thr Val Val Thr Leu Asp Gly Ser Trp Ala Ser His230 235 240 245tgg gag cac acc gtt gca gcc acc aag ggc ggc ccg cgc atc ctc acg 883Trp Glu His Thr Val Ala Ala Thr Lys Gly Gly Pro Arg Ile Leu Thr
250 255 260ccg cgt tat taaaatgatg cttttcgacg cat 915Pro Arg Tyr<210>32<211>264<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>32Met Gly Phe Arg Ser Lys Lys Lys Val Ile Ala Ala Lys Thr Ala Ala1 5 10 15Glu Leu Asp Ala Met Gln Ala Ala Gly Glu Ile Val Gly Lys Ala Leu
20 25 30Gln Ala Val Arg Ala Glu Ala Lys Ala Gly Met Ser Thr Trp Asp Leu
35 40 45Asp Gln Ile Ala Glu Gln Val Ile Arg Asp Ala Gly Ala Val Pro Thr
50 55 60Phe Leu Gly Tyr Gln Gly Phe Pro Ala Ser Val Cys Ala Ser Val Asn65 70 75 80Glu Val Ile Val His Gly Ile Pro Ser Lys Glu Thr Ile Leu Glu Glu
85 90 95Gly Asp Leu Val Ser Ile Asp Cys Gly Ala Thr Phe Asp Gly Trp Val
100 105 110Gly Asp Ser Ala Trp Ser Phe Gly Ile Gly Glu Leu Asp Glu Asp Val
115 120 125Gln Gly Leu Asn Leu Ala Thr Glu Trp Val Leu Met Glu Gly Met Lys
130 135 140Ala Met Val Pro Gly Asn Arg Leu Thr Asp Val Ser His Ala Leu Glu145 150 155 160Val Ala Thr Arg Lys Ala Glu Ser Lys Phe Gly Val Ala Leu Gly Ile
165 170 175Val Asp Gly Tyr Gly Gly His Gly Ile Gly Arg His Met His Glu Glu
180 185 190Pro Tyr Leu Ala Asn Glu Gly Lys Ala Gly Lys Gly Pro Val Ile Gln
195 200 205Glu Gly Ser Val Leu Ala Ile Glu Pro Met Leu Thr Leu Gly Thr Glu
210 215 220Asp Ser Ala Val Leu Glu Asp Asp Trp Thr Val Val Thr Leu Asp Gly225 230 235 240Ser Trp Ala Ser His Trp Glu His Thr Val Ala Ala Thr Lys Gly Gly
245 250 255Pro Arg Ile Leu Thr Pro Arg Tyr
260<210>33<211>506<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(483)<223>FRXA00675<400>33tgc ggc gca acc ttt gat ggt tgg gtc ggc gat tcc gcg tgg agc ttc 48Cys Gly Ala Thr Phe Asp Gly Trp Val Gly Asp Ser Ala Trp Ser Phe1 5 10 15ggc atc ggc gag ctg gac gag gac gtc cag ggt ctc aac ttg gct acc 96Gly Ile Gly Glu Leu Asp Glu Asp Val Gln Gly Leu Asn Leu Ala Thr
20 25 30gag tgg gtc ctc atg gaa ggc atg aag gcc atg gtt cca ggc aac cgt 144Glu Trp Val Leu Met Glu Gly Met Lys Ala Met Val Pro Gly Asn Arg
35 40 45ttg acc gat gtc tcc cac gct ctc gag gtc gca acc cgc aag gct gag 192Leu Thr Asp Val Ser His Ala Leu Glu Val Ala Thr Arg Lys Ala Glu
50 55 60tcc aag ttc ggc gtc gcg ctc ggc atc gtc gat ggc tac ggc gga cac 240Ser Lys Phe Gly Val Ala Leu Gly Ile Val Asp Gly Tyr Gly Gly His65 70 75 80ggc att ggc cgc cac atg cac gag gag cca tac ttg gct aat gag ggc 288Gly Ile Gly Arg His Met His Glu Glu Pro Tyr Leu Ala Asn Glu Gly
85 90 95aag gcc ggc aag ggc cct gtg att cag gag ggc tcc gtg ctc gcc att 336Lys Ala Gly Lys Gly Pro Val Ile Gln Glu Gly Ser Val Leu Ala Ile
100 105 110gag cct atg ctc acc ctc ggc acc gaa gat tcc gca gtg ctg gaa gat 384Glu Pro Met Leu Thr Leu Gly Thr Glu Asp Ser Ala Val Leu Glu Asp
115 120 125gat tgg act gtc gtg act ctc gac ggt tca tgg gca tca cac tgg gag 432Asp Trp Thr Val Val Thr Leu Asp Gly Ser Trp Ala Ser His Trp Glu
130 135 140cac acc gtt gca gcc acc aag ggc ggc ccg cgc atc ctc acg ccg cgt 480His Thr Val Ala Ala Thr Lys Gly Gly Pro Arg Ile Leu Thr Pro Arg145 150 155 160tat taaaatgatg cttttcgacg cat 506Tyr<210>34<211>161<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>34Cys Gly Ala Thr Phe Asp Gly Trp Val Gly Asp Ser Ala Trp Ser Phe1 5 10 15Gly Ile Gly Glu Leu Asp Glu Asp Val Gln Gly Leu Asn Leu Ala Thr
20 25 30Glu Trp Val Leu Met Glu Gly Met Lys Ala Met Val Pro Gly Asn Arg
35 40 45Leu Thr Asp Val Ser His Ala Leu Glu Val Ala Thr Arg Lys Ala Glu
50 55 60Ser Lys Phe Gly Val Ala Leu Gly Ile Val Asp Gly Tyr Gly Gly His65 70 75 80Gly Ile Gly Arg His Met His Glu Glu Pro Tyr Leu Ala Asn Glu Gly
85 90 95Lys Ala Gly Lys Gly Pro Val Ile Gln Glu Gly Ser Val Leu Ala Ile
100 105 110Glu Pro Met Leu Thr Leu Gly Thr Glu Asp Ser Ala Val Leu Glu Asp
115 120 125Asp Trp Thr Val Val Thr Leu Asp Gly Ser Trp Ala Ser His Trp Glu
130 135 140His Thr Val Ala Ala Thr Lys Gly Gly Pro Arg Ile Leu Thr Pro Arg145 150 155 160Tyr<210>35<211>996<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(973)<223>RXA01609<400>35gttgcagtca gtgaagctga agagcagcca gtcggctaaa aaatagcctc catccaaccc 60gctttacctg tcatcgcttc aggcgactag catggtgacc atg tct aaa atg cgc 115
Met Ser Lys Met Arg
1 5gca cca ctt gta ccc gga att cct acc cca atc agg gaa gta cct gca 163Ala Pro Leu Val Pro Gly Ile Pro Thr Pro Ile Arg Glu Val Pro Ala
10 15 20cat att gaa cgt cca gaa tat gtg tgg aag gac gaa gtc caa gaa gca 211His Ile Glu Arg Pro Glu Tyr Val Trp Lys Asp Glu Val Gln Glu Ala
25 30 35atc ggt gag cct ttt gtg cag gcc cct gag gtc atc gag aag atg cgt 259Ile Gly Glu Pro Phe Val Gln Ala Pro Glu Val Ile Glu Lys Met Arg
40 45 50gag aca tct cgc atc gct gca aac tca ctg aaa atc gcg ggc gaa gcc 307Glu Thr Ser Arg Ile Ala Ala Asn Ser Leu Lys Ile Ala Gly Glu Ala
55 60 65gtc aag cca ggc gtg acc act gat gaa ctt gat cgc att gtg cat gag 355Val Lys Pro Gly Val Thr Thr Asp Glu Leu Asp Arg Ile Val His Glu70 75 80 85tac acc tgc gat atg ggc gca tac cct tca gat ctt ggt tac cgg gga 403Tyr Thr Cys Asp Met Gly Ala Tyr Pro Ser Asp Leu Gly Tyr Arg Gly
90 95 100ttc acc aag tcc tca tgc att tcc ctc aat gag atc gtg tgc cac ggt 451Phe Thr Lys Ser Ser Cys Ile Ser Leu Asn Glu Ile Val Cys His Gly
105 110 115att cct gat tcc acc gtc att gaa gag ggc gat att gtt aac atc gat 499Ile Pro Asp Ser Thr Val Ile Glu Glu Gly Asp Ile Val Asn Ile Asp
120 125 130gtc acc gcg ttc aag cac ggc gtc cac ggc gac tgc aat gcc acc ttc 547Val Thr Ala Phe Lys His Gly Val His Gly Asp Cys Asn Ala Thr Phe
135 140 145tta gcg ggt gat gtt tct gaa gaa cac cgc ctg ctg gtt gag cgc acc 595Leu Ala Gly Asp Val Ser Glu Glu His Arg Leu Leu Val Glu Arg Thr150 155 160 165gaa gaa gcc atg atg cgt tcc atc cgt gca gca aag cct gga cgt gaa 643Glu Glu Ala Met Met Arg Ser Ile Arg Ala Ala Lys Pro Gly Arg Glu
170 175 180atc aac gtc att ggg cgt gtc att gag tct tac gcc aag cgt ttt ggc 691Ile Asn Val Ile Gly Arg Val Ile Glu Ser Tyr Ala Lys Arg Phe Gly
185 190 195tac aac gtg gtc cgc gat ttc acc gga cac ggc atc ggc cca act ttc 739Tyr Asn Val Val Arg Asp Phe Thr Gly His Gly Ile Gly Pro Thr Phe
200 205 210cac aac ggc ctt gtg gtg ctg cac tac gac aac act cag tac cgc gat 787His Asn Gly Leu Val Val Leu His Tyr Asp Asn Thr Gln Tyr Arg Asp
215 220 225ctg ctc gtg cca ggc atg acc ttg acc atc gag cca atg atc aac ctt 835Leu Leu Val Pro Gly Met Thr Leu Thr Ile Glu Pro Met Ile Asn Leu230 235 240 245ggt tcc ctc gac tac gag atc tgg gaa gat gat tgg act gtc caa aac 883Gly Ser Leu Asp Tyr Glu Ile Trp Glu Asp Asp Trp Thr Val Gln Asn
250 255 260gtt gac cgt aag ttc agc gcg cag ttc gag cac acc att gtc atc acc 931Val Asp Arg Lys Phe Ser Ala Gln Phe Glu His Thr Ile Val Ile Thr
265 270 275gaa gac ggc aat gag atc ctc acc ctc cca gac gat tcc gtc 973Glu Asp Gly Asn Glu Ile Leu Thr Leu Pro Asp Asp Ser Val
280 285 290taaaaacgcc taggccacaa gcc 996<210>36<211>291<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>36Met Ser Lys Met Arg Ala Pro Leu Val Pro Gly Ile Pro Thr Pro Ile1 5 10 15Arg Glu Val Pro Ala His Ile Glu Arg Pro Glu Tyr Val Trp Lys Asp
20 25 30Glu Val Gln Glu Ala Ile Gly Glu Pro Phe Val Gln Ala Pro Glu Val
35 40 45Ile Glu Lys Met Arg Glu Thr Ser Arg Ile Ala Ala Asn Ser Leu Lys
50 55 60Ile Ala Gly Glu Ala Val Lys Pro Gly Val Thr Thr Asp Glu Leu Asp65 70 75 80Arg Ile Val His Glu Tyr Thr Cys Asp Met Gly Ala Tyr Pro Ser Asp
85 90 95Leu Gly Tyr Arg Gly Phe Thr Lys Ser Ser Cys Ile Ser Leu Asn Glu
100 105 110Ile Val Cys His Gly Ile Pro Asp Ser Thr Val Ile Glu Glu Gly Asp
115 120 125Ile Val Asn Ile Asp Val Thr Ala Phe Lys His Gly Val His Gly Asp
130 135 140Cys Asn Ala Thr Phe Leu Ala Gly Asp Val Ser Glu Glu His Arg Leu145 150 155 160Leu Val Glu Arg Thr Glu Glu Ala Met Met Arg Ser Ile Arg Ala Ala
165 170 175Lys Pro Gly Arg Glu Ile Asn Val Ile Gly Arg Val Ile Glu Ser Tyr
180 185 190Ala Lys Arg Phe Gly Tyr Asn Val Val Arg Asp Phe Thr Gly His Gly
195 200 205Ile Gly Pro Thr Phe His Asn Gly Leu Val Val Leu His Tyr Asp Asn
210 215 220Thr Gln Tyr Arg Asp Leu Leu Val Pro Gly Met Thr Leu Thr Ile Glu225 230 235 240Pro Met Ile Asn Leu Gly Ser Leu Asp Tyr Glu Ile Trp Glu Asp Asp
245 250 255Trp Thr Val Gln Asn Val Asp Arg Lys Phe Ser Ala Gln Phe Glu His
260 265 270Thr Ile Val Ile Thr Glu Asp Gly Asn Glu Ile Leu Thr Leu Pro Asp
275 280 285Asp Ser Val
290<210>37<211>1644<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1621)<223>RXA01358<400>37gagggtaaac cttatactcc gattcgtttt gatgtggtgg caatcgtttt ggatccgcac 60acggggcgcc cagagatcac tgtttatgag gatgtggaac atg gcg ctc ggt aga 115
Met Ala Leu Gly Arg
1 5act ata tcc act gcg caa tta ggt gtg cag gca aaa atc gtt cgt gtg 163Thr Ile Ser Thr Ala Gln Leu Gly Val Gln Ala Lys Ile Val Arg Val
10 15 20gag gct aat gtt ggc cca gga ttg cct ggt acc tac att gtt gga tta 211Glu Ala Asn Val Gly Pro Gly Leu Pro Gly Thr Tyr Ile Val Gly Leu
25 30 35gca gat act gcc att tca gaa tct cgg gat cgt att aaa act gcg gtg 259Ala Asp Thr Ala Ile Ser Glu Ser Arg Asp Arg Ile Lys Thr Ala Val
40 45 50caa aac agt ggc ttg atg tgg cca aag acc aaa gtg atc att aac ctc 307Gln Asn Ser Gly Leu Met Trp Pro Lys Thr Lys Val Ile Ile Asn Leu
55 60 65tcg ccg gct tcc atg cgt aaa caa ggt tcg cag tgt gat ctc gcg atg 355Ser Pro Ala Ser Met Arg Lys Gln Gly Set Gln Cys Asp Leu Ala Met70 75 80 85acc gtt gca gtt ctc gtt gcc cat ggc tct aac ccc aaa gcg aag ttt 403Thr Val Ala Val Leu Val Ala His Gly Ser Asn Pro Lys Ala Lys Phe
90 95 100cat gcg cag aac acg tta ttt ctg ggt gag gtg gcg ctt gat gga acc 451His Ala Gln Asn Thr Leu Phe Leu Gly Glu Val Ala Leu Asp Gly Thr
105 110 115ttg ctc cct gtt act gga gtt ctt cca gcg ctg ttg gcc gcg aag gag 499Leu Leu Pro Val Thr Gly Val Leu Pro Ala Leu Leu Ala Ala Lys Glu
120 125 130gaa ggt att ggc aag att gtg atc ccg gag gga aat gcc caa gaa gca 547Glu Gly Ile Gly Lys Ile Val Ile Pro Glu Gly Asn Ala Gln Glu Ala
135 140 145gga ctg gtt gag gat cct tca gtc ttt ttg gca cat tcc atc gac cag 595Gly Leu Val Glu Asp Pro Ser Val Phe Leu Ala His Ser Ile Asp Gln150 155 160 165gtc ttg cga tgg ctt gat ggg gaa gag gcg ttg cct cag cct gga cta 643Val Leu Arg Trp Leu Asp Gly Glu Glu Ala Leu Pro Gln Pro Gly Leu
170 175 180ttc aat gat gaa aat agc ctc aaa ctg cct gat atg cgt gat gtg gtg 691Phe Asn Asp Glu Asn Ser Leu Lys Leu Pro Asp Met Arg Asp Val Val
185 190 195gga caa cca gaa gct agg ttt gct gca gaa gta gct gct gcc ggt ggt 739Gly Gln Pro Glu Ala Arg Phe Ala Ala Glu Val Ala Ala Ala Gly Gly
200 205 210cac cac atg ctg atg att ggc cct ccc ggt tct gga aaa tcc atg atc 787His His Met Leu Met Ile Gly Pro Pro Gly Ser Gly Lys Ser Met Ile
215 220 225gcc gag cgg att ccc agc ttg ctt ccc gaa ctg tcg ccc caa caa atg 835Ala Glu Arg Ile Pro Ser Leu Leu Pro Glu Leu Ser Pro Gln Gln Met230 235 240 245atc gag gcg acg gca gtg cat tcc gtt gtg ggg cga acc ttt tca ggg 883Ile Glu Ala Thr Ala Val His Ser Val Val Gly Arg Thr Phe Ser Gly
250 255 260ccg gtg tcg agg gct ccg ttt att tcc cca cac cac aat gtc agc aag 931Pro Val Ser Arg Ala Pro Phe Ile Ser Pro His His Asn Val Ser Lys
265 270 275gct gct ctg ctc gga ggt ggc tcg gga tct cct ctt ccg ggt gct atc 979Ala Ala Leu Leu Gly Gly Gly Ser Gly Ser Pro Leu Pro Gly Ala Ile
280 285 290agc ctg gcg cat cat ggt gtg ttg ttc ctg gat gag gtt agt gag att 1027Ser Leu Ala His His Gly Val Leu Phe Leu Asp Glu Val Ser Glu Ile
295 300 305cca gcg tca atc ctt gat tct ttg agg act cca ttg gaa tac ggc tcg 1075Pro Ala Ser Ile Leu Asp Ser Leu Arg Thr Pro Leu Glu Tyr Gly Ser310 315 320 325atc cgc atc atc aga tcc cgc cat gat gtc acc ttc ccc gca cag ttc 1123Ile Arg Ile Ile Arg Ser Arg His Asp Val Thr Phe Pro Ala Gln Phe
330 335 340cag ctc atc ctc gcg gcc aat ccg tgt aga tgc ggt gca gaa cag cct 1171Gln Leu Ile Leu Ala Ala Asn Pro Cys Arg Cys Gly Ala Glu Gln Pro
345 350 355caa gaa tgt gtc tgt tct ggc tca gct cgc gcg acg tac ctt aat aat 1219Gln Glu Cys Val Cys Ser Gly Ser Ala Arg Ala Thr Tyr Leu Asn Asn
360 365 370ctt tcg ggt ccg ttg agg gat cgc ttg gac atg gtt gtt gcc acc cac 1267Leu Ser Gly Pro Leu Arg Asp Arg Leu Asp Met Val Val Ala Thr His
375 380 385tct aaa ggt gca gtg ctg cgt agt gat gac gtt gag gca tct gct ccc 1315Ser Lys Gly Ala Val Leu Arg Ser Asp Asp Val Glu Ala Ser Ala Pro390 395 400 405att gct gat cgg gtg gca caa gct cgt gag agg gca gct ttc cga tgg 1363Ile Ala Asp Arg Val Ala Gln Ala Arg Glu Arg Ala Ala Phe Arg Trp
410 415 420cgc cgt tct gga ctg gga aat ctt gtt aat gca cac gta gat cca cac 1411Arg Arg Ser Gly Leu Gly Asn Leu Val Asn Ala His Val Asp Pro His
425 430 435ttc ttg cgg agg aat ttt gcc gca aca gaa gac gcc atg gtg tac ctc 1459Phe Leu Arg Arg Asn Phe Ala Ala Thr Glu Asp Ala Met Val Tyr Leu
440 445 450ggc gcg ttc ctg gcg gaa gga aca atc tcc caa cgt ggc tgc gat cgg 1507Gly Ala Phe Leu Ala Glu Gly Thr Ile Ser Gln Arg Gly Cys Asp Arg
455 460 465gcc ata aaa ctg ggt tgg acc ttg tgc gat ttg gat ggg gaa cag cag 1555Ala Ile Lys Leu Gly Trp Thr Leu Cys Asp Leu Asp Gly Glu Gln Gln470 475 480 485ccc aat ctt gac cat att gcg cga gcc atg gag ctt cgg ggc act aca 1603Pro Asn Leu Asp His Ile Ala Arg Ala Met Glu Leu Arg Gly Thr Thr
490 495 500tac agt gag gta gca gca tgattgactc ccgcttgctg gca 1644Tyr Ser Glu Val Ala Ala
505<210>38<211>507<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>38Met Ala Leu Gly Arg Thr Ile Ser Thr Ala Gln Leu Gly Val Gln Ala1 5 10 15Lys Ile Val Arg Val Glu Ala Asn Val Gly Pro Gly Leu Pro Gly Thr
20 25 30Tyr Ile Val Gly Leu Ala Asp Thr Ala Ile Ser Glu Ser Arg Asp Arg
35 40 45Ile Lys Thr Ala Val Gln Asn Ser Gly Leu Met Trp Pro Lys Thr Lys
50 55 60Val Ile Ile Asn Leu Ser Pro Ala Ser Met Arg Lys Gln Gly Ser Gln65 70 75 80Cys Asp Leu Ala Met Thr Val Ala Val Leu Val Ala His Gly Ser Asn
85 90 95Pro Lys Ala Lys Phe His Ala Gln Asn Thr Leu Phe Leu Gly Glu Val
100 105 110Ala Leu Asp Gly Thr Leu Leu Pro Val Thr Gly Val Leu Pro Ala Leu
115 120 125Leu Ala Ala Lys Glu Glu Gly Ile Gly Lys Ile Val Ile Pro Glu Gly
130 135 140Asn Ala Gln Glu Ala Gly Leu Val Glu Asp Pro Ser Val Phe Leu Ala145 150 155 160His Ser Ile Asp Gln Val Leu Arg Trp Leu Asp Gly Glu Glu Ala Leu
165 170 175Pro Gln Pro Gly Leu Phe Asn Asp Glu Asn Ser Leu Lys Leu Pro Asp
180 185 190Met Arg Asp Val Val Gly Gln Pro Glu Ala Arg Phe Ala Ala Glu Val
195 200 205Ala Ala Ala Gly Gly His His Met Leu Met Ile Gly Pro Pro Gly Ser
210 215 220Gly Lys Ser Met Ile Ala Glu Arg Ile Pro Ser Leu Leu Pro Glu Leu225 230 235 240Ser Pro Gln Gln Met Ile Glu Ala Thr Ala Val His Ser Val Val Gly
245 250 255Arg Thr Phe Ser Gly Pro Val Ser Arg Ala Pro Phe Ile Ser Pro His
260 265 270His Asn Val Ser Lys Ala Ala Leu Leu Gly Gly Gly Ser Gly Ser Pro
275 280 285Leu Pro Gly Ala Ile Ser Leu Ala His His Gly Val Leu Phe Leu Asp
290 295 300Glu Val Ser Glu Ile Pro Ala Ser Ile Leu Asp Ser Leu Arg Thr Pro305 310 315 320Leu Glu Tyr Gly Ser Ile Arg Ile Ile Arg Ser Arg His Asp Val Thr
325 330 335Phe Pro Ala Gln Phe Gln Leu Ile Leu Ala Ala Asn Pro Cys Arg Cys
340 345 350Gly Ala Glu Gln Pro Gln Glu Cys Val Cys Ser Gly Ser Ala Arg Ala
355 360 365Thr Tyr Leu Asn Asn Leu Ser Gly Pro Leu Arg Asp Arg Leu Asp Met
370 375 380Val Val Ala Thr His Ser Lys Gly Ala Val Leu Arg Ser Asp Asp Val385 390 395 400Glu Ala Ser Ala Pro Ile Ala Asp Arg Val Ala Gln Ala Arg Glu Arg
405 410 415Ala Ala Phe Arg Trp Arg Arg Ser Gly Leu Gly Asn Leu Val Asn Ala
420 425 430His Val Asp Pro His Phe Leu Arg Arg Asn Phe Ala Ala Thr Glu Asp
435 440 445Ala Met Val Tyr Leu Gly Ala Phe Leu Ala Glu Gly Thr Ile Ser Gln
450 455 460Arg Gly Cys Asp Arg Ala Ile Lys Leu Gly Trp Thr Leu Cys Asp Leu465 470 475 480Asp Gly Glu Gln Gln Pro Asn Leu Asp His Ile Ala Arg Ala Met Glu
485 490 495Leu Arg Gly Thr Thr Tyr Ser Glu Val Ala Ala
500 505<210>39<211>1173<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1150)<223>RXA01458<400>39gccattagag ttcatattta ccaatctaca aggcaactat ttcctattgc tacgatccga 60acaacgctgt aagcgaacac aggttgtagg gtggatattc gtg aat cgc cga atc 115
Val Asn Arg Arg Ile
1 5aag act ctg acg tgg ggt gct atc cct ttg gtg ctg ctg gca tcg ttg 163Lys Thr Leu Thr Trp Gly Ala Ile Pro Leu Val Leu Leu Ala Ser Leu
10 15 20gta agc att gac cat att ccg gga aca aac atc aac ttg agc gtg cct 211Val Ser Ile Asp His Ile Pro Gly Thr Asn Ile Asn Leu Ser Val Pro
25 30 35tat gcc gct gaa ggc cca ggt cct acg atc aat acg ctt ggt cag gtc 259Tyr Ala Ala Glu Gly Pro Gly Pro Thr Ile Asn Thr Leu Gly Gln Val
40 45 50gac ggc gag gat gtt gtg tcc atc agt agt gct gat ctg gat gag acc 307Asp Gly Glu Asp Val Val Ser Ile Ser Ser Ala Asp Leu Asp Glu Thr
55 60 65gaa ggt aac ctg aac atg acc act gtg tcg gtt cgt tcc ggc atg aca 355Glu Gly Asn Leu Asn Met Thr Thr Val Ser Val Arg Ser Gly Met Thr70 75 80 85ttg tcg cag gta att tcc cga tgg ctg ttt acc gat gac aca atc gtt 403Leu Ser Gln Val Ile Ser Arg Trp Leu Phe Thr Asp Asp Thr Ile Val
90 95 100ccc atc gag cag gtt ttc cct ccc ggc caa tcc acc gag gaa gtc gaa 451Pro Ile Glu Gln Val Phe Pro Pro Gly Gln Ser Thr Glu Glu Val Glu
105 110 115gaa tcc aac cgc acc gcg ttc atc tct tcg gag tct tcc gca acg atc 499Glu Ser Asn Arg Thr Ala Phe Ile Ser Ser Glu Ser Ser Ala Thr Ile
120 125 130gcc gcg atg aat tac ctc aac att ccc gtc gaa gtt gaa gtt gca gaa 547Ala Ala Met Asn Tyr Leu Asn Ile Pro Val Glu Val Glu Val Ala Glu
135 140 145gtc ctc acc gac agc gcc gca acc gga att ttc gaa ccc ggc gac aaa 595Val Leu Thr Asp Ser Ala Ala Thr Gly Ile Phe Glu Pro Gly Asp Lys150 155 160 165ctt ctc agc atc gac ggc acc gca atc tcc act ccc ggc gat gca caa 643Leu Leu Ser Ile Asp Gly Thr Ala Ile Ser Thr Pro Gly Asp Ala Gln
170 175 180acc atc gtg cga tcg aaa gct ccc ggc gat gag atc acg att tcc tac 691Thr Ile Val Arg Ser Lys Ala Pro Gly Asp Glu Ile Thr Ile Ser Tyr
185 190 195gag aga aac gat gcg gaa tct caa gca acc atc act ttg agg gaa cac 739Glu Arg Asn Asp Ala Glu Ser Gln Ala Thr Ile Thr Leu Arg Glu His
200 205 210ccg gat gat tct tcg gtg gcg ttg ttg ggt att tca atg ttg tcg gtg 787Pro Asp Asp Ser Ser Val Ala Leu Leu Gly Ile Ser Met Leu Ser Val
215 220 225cct tcg agc gcg att gag gtt gat tac aac ttg gaa gat atc ggt ggt 835Pro Ser Ser Ala Ile Glu Val Asp Tyr Asn Leu Glu Asp Ile Gly Gly230 235 240 245ccg agc gct ggc atg atg ttt tcg ttg gcg gtc gtc gat aag ctt tcg 883Pro Ser Ala Gly Met Met Phe Ser Leu Ala Val Val Asp Lys Leu Ser
250 255 260cct ggc gcg ctg aat ggt ggc aag ttt gtc gct ggc act ggc acc atc 931Pro Gly Ala Leu Asn Gly Gly Lys Phe Val Ala Gly Thr Gly Thr Ile
265 270 275gcg gag gac ggg tcg gtg ggc ccg att ggc ggt att gcg cac aag gtg 979Ala Glu Asp Gly Ser Val Gly Pro Ile Gly Gly Ile Ala His Lys Val
280 285 290cgc gct gcg gag gac gcg ggc gcg gaa gtg ttt ttg agc cct gcg gac 1027Arg Ala Ala Glu Asp Ala Gly Ala Glu Val Phe Leu Ser Pro Ala Asp
295 300 305aat tgc gcg gag gcg atg agt gcg aag cct cag gat atg acg atc ttg 1075Asn Cys Ala Glu Ala Met Ser Ala Lys Pro Gln Asp Met Thr Ile Leu310 315 320 325aag gtg gat tcg ttg tct cag gca atc gat cag atg gct gcc tac aac 1123Lys Val Asp Ser Leu Ser Gln Ala Ile Asp Gln Met Ala Ala Tyr Asn
330 335 340gag ggc tct gat ttc cag acg tgt ggc tagtttttag acgctgaggt 1170Glu Gly Ser Asp Phe Gln Thr Cys Gly
345 350ttc1173
<210>40<211>350<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>40Val Asn Arg Arg Ile Lys Thr Leu Thr Trp Gly Ala Ile Pro Leu Val1 5 10 15Leu Leu Ala Ser Leu Val Ser Ile Asp His Ile Pro Gly Thr Asn Ile
20 25 30Asn Leu Ser Val Pro Tyr Ala Ala Glu Gly Pro Gly Pro Thr Ile Asn
35 40 45Thr Leu Gly Gln Val Asp Gly Glu Asp Val Val Ser Ile Ser Ser Ala
50 55 60Asp Leu Asp Glu Thr Glu Gly Asn Leu Asn Met Thr Thr Val Ser Val65 70 75 80Arg Ser Gly Met Thr Leu Ser Gln Val Ile Ser Arg Trp Leu Phe Thr
85 90 95Asp Asp Thr Ile Val Pro Ile Glu Gln Val Phe Pro Pro Gly Gln Ser
100 105 110Thr Glu Glu Val Glu Glu Ser Asn Arg Thr Ala Phe Ile Ser Ser Glu
115 120 125Ser Ser Ala Thr Ile Ala Ala Met Asn Tyr Leu Asn Ile Pro Val Glu
130 135 140Val Glu Val Ala Glu Val Leu Thr Asp Ser Ala Ala Thr Gly Ile Phe145 150 155 160Glu Pro Gly Asp Lys Leu Leu Ser Ile Asp Gly Thr Ala Ile Ser Thr
165 170 175Pro Gly Asp Ala Gln Thr Ile Val Arg Ser Lys Ala Pro Gly Asp Glu
180 185 190Ile Thr Ile Ser Tyr Glu Arg Asn Asp Ala Glu Ser Gln Ala Thr Ile
195 200 205Thr Leu Arg Glu His Pro Asp Asp Ser Ser Val Ala Leu Leu Gly Ile
210 215 220Ser Met Leu Ser Val Pro Ser Ser Ala Ile Glu Val Asp Tyr Asn Leu225 230 235 240Glu Asp Ile Gly Gly Pro Ser Ala Gly Met Met Phe Ser Leu Ala Val
245 250 255Val Asp Lys Leu Ser Pro Gly Ala Leu Asn Gly Gly Lys Phe Val Ala
260 265 270Gly Thr Gly Thr Ile Ala Glu Asp Gly Ser Val Gly Pro Ile Gly Gly
275 280 285Ile Ala His Lys Val Arg Ala Ala Glu Asp Ala Gly Ala Glu Val Phe
290 295 300Leu Ser Pro Ala Asp Asn Cys Ala Glu Ala Met Ser Ala Lys Pro Gln305 310 315 320Asp Met Thr Ile Leu Lys Val Asp Ser Leu Ser Gln Ala Ile Asp Gln
325 330 335Met Ala Ala Tyr Asn Glu Gly Ser Asp Phe Gln Thr Cys Gly
340 345 350<210>41<211>1119<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1096)<223>RXA01654<400>41cttttgctgt tatgaaagtg aactaaagat atctatgcgc agaccggtct cacgccgcgc 60catttttgca acatctgttt tggttgcggg ggtgagcatc atg tca cct tcg gcc 115
Met Ser Pro Ser Ala
1 5aac gca gct gag gct ccg gca tcg gaa tgg gtg aat acg aca gcg atc 163Asn Ala Ala Glu Ala Pro Ala Ser Glu Trp Val Asn Thr Thr Ala Ile
10 15 20gta gat caa gcg aat gct cag ttg tcg cag ttt ggc gtg agt ctt gac 211Val Asp Gln Ala Asn Ala Gln Leu Ser Gln Phe Gly Val Ser Leu Asp
25 30 35cga agt gca gca gaa ctt ttt gat gat cag gca aac tcc caa att gat 259Arg Ser Ala Ala Glu Leu Phe Asp Asp Gln Ala Asn Ser Gln Ile Asp
40 45 50gca gcg ctt tca ccg tat gcc gat aag gtt cca acc tct ggc ggc cag 307Ala Ala Leu Ser Pro Tyr Ala Asp Lys Val Pro Thr Ser Gly Gly Gln
55 60 65gta gtc gag caa agt ctt cag gtt gtg gag cag gaa gtt caa aag gca 355Val Val Glu Gln Ser Leu Gln Val Val Glu Gln Glu Val Gln Lys Ala70 75 80 85ctg ccc aac tat gaa atc cgt acc gat ctg caa tcc cag gtg atg ggt 403Leu Pro Asn Tyr Glu Ile Arg Thr Asp Leu Gln Ser Gln Val Met Gly
90 95 100gca act cta gga gag gtg ctg cac cga gtt cct gga tca tgg ttt gat 451Ala Thr Leu Gly Glu Val Leu His Arg Val Pro Gly Ser Trp Phe Asp
105 110 115gcg cca gca gtt cct gaa gaa tcc agg att gta gag gaa cag ggt aaa 499Ala Pro Ala Val Pro Glu Glu Ser Arg Ile Val Glu Glu Gln Gly Lys
120 125 130tcc ctg tat ggg ccc ggt acc ccg atc tat ctc aac gga aat tcc atg 547Ser Leu Tyr Gly Pro Gly Thr Pro Ile Tyr Leu Asn Gly Asn Ser Met
135 140 145tgc acg ctt gcg gtg act gga act gat gca gat ggg cgc aag atc ggt 595Cys Thr Leu Ala Val Thr Gly Thr Asp Ala Asp Gly Arg Lys Ile Gly150 155 160 165atc act gca ggt cac tgt gga aaa tcg ggc gat gca gtc cgt tcg gct 643Ile Thr Ala Gly His Cys Gly Lys Ser Gly Asp Ala Val Arg Ser Ala
170 175 180gac tcc ttc tgg gtc ggc gat acc gga aca gtg gtg tac aac gcg cct 691Asp Ser Phe Trp Val Gly Asp Thr Gly Thr Val Val Tyr Asn Ala Pro
185 190 195aac gct gac tac tcc gtg atc gag ttc ggt tcc aat gca gag ttg agc 739Asn Ala Asp Tyr Ser Val Ile Glu Phe Gly Ser Asn Ala Glu Leu Ser
200 205 210aat acc tac aac ggt gtc acc gcg aat gct gtc ggc ggt ggc gtg act 787Asn Thr Tyr Asn Gly Val Thr Ala Asn Ala Val Gly Gly Gly Val Thr
215 220 225aat ggc caa gaa gta tgc aaa aac gga gtt gct act ggc tac acc tgt 835Asn Gly Gln Glu Val Cys Lys Asn Gly Val Ala Thr Gly Tyr Thr Cys230 235 240 245ggt ttg gtg tgg act gct gat gag cgc atg acg atg tct cag gtg tgt 883Gly Leu Val Trp Thr Ala Asp Glu Arg Met Thr Met Ser Gln Val Cys
250 255 260gcg ggt cgt ggt gat tcg ggt gct ccg ctg att gca gat ggt cgt gtg 931Ala Gly Arg Gly Asp Ser Gly Ala Pro Leu Ile Ala Asp Gly Arg Val
265 270 275gtt ggt ctt gta tct ggt ggt gta att cct gat tac aac ctg gca tgc 979Val Gly Leu Val Ser Gly Gly Val Ile Pro Asp Tyr Asn Leu Ala Cys
280 285 290gcc act ccg ttg cag gga cct ttc ttc atg cca acg ctg tca gtg aac 1027Ala Thr Pro Leu Gln Gly Pro Phe Phe Met Pro Thr Leu Ser Val Asn
295 300 305atg gat act gtc cta act gat ttg gat tcg cag gat ctt ccc ggt cga 1075Met Asp Thr Val Leu Thr Asp Leu Asp Ser Gln Asp Leu Pro Gly Arg310 315 320 325ggt ttt cag cca act gct gga tagaatttag aaaatccgcc gtt 1119Gly Phe Gln Pro Thr Ala Gly
330<210>42<211>332<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400> 42Met Ser Pro Ser Ala Asn Ala Ala Glu Ala Pro Ala Ser Glu Trp Val1 5 10 15Asn Thr Thr Ala Ile Val Asp Gln Ala Asn Ala Gln Leu Ser Gln Phe
20 25 30Gly Val Ser Leu Asp Arg Ser Ala Ala Glu Leu Phe Asp Asp Gln Ala
35 40 45Ash Ser Gln Ile Asp Ala Ala Leu Ser Pro Tyr Ala Asp Lys Val Pro
50 55 60Thr Ser Gly Gly Gln Val Val Glu Gln Ser Leu Gln Val Val Glu Gln65 70 75 80Glu Val Gln Lys Ala Leu Pro Asn Tyr Glu Ile Arg Thr Asp Leu Gln
85 90 95Ser Gln Val Met Gly Ala Thr Leu Gly Glu Val Leu His Arg Val Pro
100 105 110Gly Ser Trp Phe Asp Ala Pro Ala Val Pro Glu Glu Ser Arg Ile Val
115 120 125Glu Glu Gln Gly Lys Ser Leu Tyr Gly Pro Gly Thr Pro Ile Tyr Leu
130 135 140Asn Gly Asn Ser Met Cys Thr Leu Ala Val Thr Gly Thr Asp Ala Asp145 150 155 160Gly Arg Lys Ile Gly Ile Thr Ala Gly His Cys Gly Lys Ser Gly Asp
165 170 175Ala Val Arg Ser Ala Asp Ser Phe Trp Val Gly Asp Thr Gly Thr Val
180 185 190Val Tyr Asn Ala Pro Asn Ala Asp Tyr Ser Val Ile Glu Phe Gly Ser
195 200 205Asn Ala Glu Leu Ser Asn Thr Tyr Asn Gly Val Thr Ala Asn Ala Val
210 215 220Gly Gly Gly Val Thr Asn Gly Gln Glu Val Cys Lys Asn Gly Val Ala225 230 235 240Thr Gly Tyr Thr Cys Gly Leu Val Trp Thr Ala Asp Glu Arg Met Thr
245 250 255Met Ser Gln Val Cys Ala Gly Arg Gly Asp Ser Gly Ala Pro Leu Ile
260 265 270Ala Asp Gly Arg Val Val Gly Leu Val Ser Gly Gly Val Ile Pro Asp
275 280 285Tyr Asn Leu Ala Cys Ala Thr Pro Leu Gln Gly Pro Phe Phe Met Pro
290 295 300Thr Leu Ser Val Asn Met Asp Thr Val Leu Thr Asp Leu Asp Ser Gln305 310 315 320Asp Leu Pro Gly Arg Gly Phe Gln Pro Thr Ala Gly
325 330<210>43<211>2049<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (2026)<223>RXN01868<400>43tgacaggcta ccttctgggg tggacatgat ccccaacgct caacccactt gtggcaccaa 60ccacaaaccc tgtggcggta aatcccctag agtaggccac atg aag gat ctt tat 115
Met Lys Asp Leu Tyr
1 5cgc ttt gtc aat ggc ctg tgg ctt gac acc cac atc att ccc gac gat 163Arg Phe Val Asn Gly Leu Trp Leu Asp Thr His Ile Ile Pro Asp Asp
10 15 20cgc gcg gtg gac ggc acg ttc cac aag ctg cgc gat gat gct gaa gaa 211Arg Ala Val Asp Gly Thr Phe His Lys Leu Arg Asp Asp Ala Glu Glu
25 30 35gac gtc cat gag atc gtc aag gaa gac act gga cgc gca ggc aca ctt 259Asp Val His Glu Ile Val Lys Glu Asp Thr Gly Arg Ala Gly Thr Leu
40 45 50tat gcc tca ttt atg gat act gac gcc atc aac gct gct ggt gtt gca 307Tyr Ala Ser Phe Met Asp Thr Asp Ala Ile Asn Ala Ala Gly Val Ala
55 60 65ccg ctc gat gcg gat ctg aac agg ctg tct gtt gct aac tca tcg ttt 355Pro Leu Asp Ala Asp Leu Asn Arg Leu Ser Val Ala Asn Ser Ser Phe70 75 80 85ttc gca gct gct ctc ggc gaa ctg gac cgt gaa ggc gtt ggc gcg cca 403Phe Ala Ala Ala Leu Gly Glu Leu Asp Arg Glu Gly Val Gly Ala Pro
90 95 100gta ggt ttc tgg gtg gag aag gat tct tcc tcc aac gaa tcc gtc gcc 451Val Gly Phe Trp Val Glu Lys Asp Ser Ser Ser Asn Glu Ser Val Ala
105 110 115tat gtc atc cag tcc ggc ctc ggc ctg ccc gat gag gct tat tac cgc 499Tyr Val Ile Gln Ser Gly Leu Gly Leu Pro Asp Glu Ala Tyr Tyr Arg
120 125 130gag gag gca cac gcc gaa act ctc gcg gcc tac aaa gag cac gtt gag 547Glu Glu Ala His Ala Glu Thr Leu Ala Ala Tyr Lys Glu His Val Glu
135 140 145cgc atg ctc ggc tac ttg gat aac agc cgc ctc ttc ggt ctg tcg gct 595Arg Met Leu Gly Tyr Leu Asp Asn Ser Arg Leu Phe Gly Leu Ser Ala150 155 160 165gct tcc gct gcc gca cga att gtc gcc ctg gaa acg gaa atc gct gct 643Ala Ser Ala Ala Ala Arg Ile Val Ala Leu Glu Thr Glu Ile Ala Ala
170 175 180ggc cac tgg gat gtc gtg aag acc cgc gac gcc gta gcc acc tac aac 691Gly His Trp Asp Val Val Lys Thr Arg Asp Ala Val Ala Thr Tyr Asn
185 190 195ccc acc gaa ctc ggc gcg ctg cca cca aag gtc cgc acg ctg ctc agt 739Pro Thr Glu Leu Gly Ala Leu Pro Pro Lys Val Arg Thr Leu Leu Ser
200 205 210tcc gca ggc ctc ccg gac cag cgc ctg gta tcg atg atg ccg tca tac 787Ser Ala Gly Leu Pro Asp Gln Arg Leu Val Ser Met Met Pro Ser Tyr
215 220 225ctc gac cac ctc aac ggc ttg ctt gtc gac gac cgc ctc ccc gat tgg 835Leu Asp His Leu Asn Gly Leu Leu Val Asp Asp Arg Leu Pro Asp Trp230 235 240 245cag cta tgg gca acc tgg cac atc ttg agg tct cga gca gga ctg ttg 883Gln Leu Trp Ala Thr Trp His Ile Leu Arg Ser Arg Ala Gly Leu Leu
250 255 260acc gag gaa att agc caa gca aac ttc gac ttc tat ggc acc aaa ctg 931Thr Glu Glu Ile Ser Gln Ala Asn Phe Asp Phe Tyr Gly Thr Lys Leu
265 270 275tcc ggc gcc acc gag caa aaa gat cga tgg aag cgt gct gtc ggc ctg 979Ser Gly Ala Thr Glu Gln Lys Asp Arg Trp Lys Arg Ala Val Gly Leu
280 285 290gca gag cgc atg gtg ggc gag gaa atc ggg caa cga ttc gtc gaa agg 1027Ala Glu Arg Met Val Gly Glu Glu Ile Gly Gln Arg Phe Val Glu Arg
295 300 305cat ttt cct gca agc tcc aag gag cac atg ctt gag ctc gtc gac tac 1075His Phe Pro Ala Ser Ser Lys Glu His Met Leu Glu Leu Val Asp Tyr310 315 320 325ctg gtt gcc gcc tac cgt gat cgc att tcc aac ctc gaa tgg atg acg 1123Leu Val Ala Ala Tyr Arg Asp Arg Ile Ser Asn Leu Glu Trp Met Thr
330 335 340ccc gcc acc cgc gag cgt gcc ctg gaa aag ttg ggc aaa ttc aac gcg 1171Pro Ala Thr Arg Glu Arg Ala Leu Glu Lys Leu Gly Lys Phe Asn Ala
345 350 355aaa atc ggc tac ccc gac aag tgg cgc tcc tac gaa ggc ctc gaa ttc 1219Lys Ile Gly Tyr Pro Asp Lys Trp Arg Ser Tyr Glu Gly Leu Glu Phe
360 365 370ggc tcc gac ctg gtg gac aac tcc cgc aag ggc tcc gcg ttc ctc cat 1267Gly Ser Asp Leu Val Asp Asn Ser Arg Lys Gly Ser Ala Phe Leu His
375 380 385gac tat gag ctg ggc aag atc ggc aaa cca gcc gac cgc gac gaa tgg 1315Asp Tyr Glu Leu Gly Lys Ile Gly Lys Pro Ala Asp Arg Asp Glu Trp390 395 400 405gtc acc acc cca caa acc gtc aac gcc ttc tac aac ccc gtg gtc aac 1363Val Thr Thr Pro Gln Thr Val Asn Ala Phe Tyr Asn Pro Val Val Asn
410 415 420gac atc acc ttc ccc gca gcc atc ctg cgc gca cca ttc ttc gac ccc 1411Asp Ile Thr Phe Pro Ala Ala Ile Leu Arg Ala Pro Phe Phe Asp Pro
425 430 435gaa gca gaa gcc gca gaa aac ttc ggt gca atc ggt gct gtg atc gga 1459Glu Ala Glu Ala Ala Glu Asn Phe Gly Ala Ile Gly Ala Val Ile Gly
440 445 450cac gaa atc ggc cac ggc ttt gac gat caa ggc agc caa tac gac ggc 1507His Glu Ile Gly His Gly Phe Asp Asp Gln Gly Ser Gln Tyr Asp Gly
455 460 465gac ggc aac ctc aac tcc tgg tgg acc gac gaa gac cgc tcc gca ttc 1555Asp Gly Asn Leu Asn Ser Trp Trp Thr Asp Glu Asp Arg Ser Ala Phe470 475 480 485gag cag ctc acc tca cgt ctg gtc acc caa ttc agc gga ctc gtc cct 1603Glu Gln Leu Thr Ser Arg Leu Val Thr Gln Phe Ser Gly Leu Val Pro
490 495 500gcc gtc ctg acc tct gaa gga atc gac acc gac ggc gtc aac ggt gaa 1651Ala Val Leu Thr Ser Glu Gly Ile Asp Thr Asp Gly Val Asn Gly Glu
505 510 515ttc act ctc ggc gaa aac atc ggt gac ctc ggc gga ttg ggc atc gct 1699Phe Thr Leu Gly Glu Asn Ile Gly Asp Leu Gly Gly Leu Gly Ile Ala
520 525 530gtc gtt gcc tac gaa aag tac ctc gca gac cgt ggc caa acc ttt gaa 1747Val Val Ala Tyr Glu Lys Tyr Leu Ala Asp Arg Gly Gln Thr Phe Glu
535 540 545acc tca cca gtc caa aaa ttc gaa gca gaa ggc gcc gag gaa ggc ctg 1795Thr Ser Pro Val Gln Lys Phe Glu Ala Glu Gly Ala Glu Glu Gly Leu550 555 560 565gcc gag caa gaa ttc aac ggt ctc caa cgc ctc ttc ctg tcc tgg gct 1843Ala Glu Gln Glu Phe Asn Gly Leu Gln Arg Leu Phe Leu Ser Trp Ala
570 575 580cgc gtg tgg cgc acc aaa atc cgc cca cag atg gcc gtc caa tac ctg 1891Arg Val Trp Arg Thr Lys Ile Arg Pro Gln Met Ala Val Gln Tyr Leu
585 590 595gcc atc gac cca cac tcc cct gca gaa ttc cgc tgc aat gtc atc gcc 1939Ala Ile Asp Pro His Ser Pro Ala Glu Phe Arg Cys Asn Val Ile Ala
600 605 610gga aac gtc gct gaa ttc tac gaa gca ttc gac gtc ccc gaa gat gca 1987Gly Asn Val Ala Glu Phe Tyr Glu Ala Phe Asp Val Pro Glu Asp Ala
615 620 625cct gtg tac atc aag cca gaa gag cgc cta gct atc tgg tagttgttag 2036Pro Val Tyr Ile Lys Pro Glu Glu Arg Leu Ala Ile Trp630 635 640ttggtattga aaa 2049<210>44<211>642<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>44Met Lys Asp Leu Tyr Arg Phe Val Asn Gly Leu Trp Leu Asp Thr His1 5 10 15Ile Ile Pro Asp Asp Arg Ala Val Asp Gly Thr Phe His Lys Leu Arg
20 25 30Asp Asp Ala Glu Glu Asp Val His Glu Ile Val Lys Glu Asp Thr Gly
35 40 45Arg Ala Gly Thr Leu Tyr Ala Ser Phe Met Asp Thr Asp Ala Ile Asn
50 55 60Ala Ala Gly Val Ala Pro Leu Asp Ala Asp Leu Asn Arg Leu Ser Val65 70 75 80Ala Asn Ser Ser Phe Phe Ala Ala Ala Leu Gly Glu Leu Asp Arg Glu
85 90 95Gly Val Gly Ala Pro Val Gly Phe Trp Val Glu Lys Asp Ser Ser Ser
100 105 110Asn Glu Ser Val Ala Tyr Val Ile Gln Ser Gly Leu Gly Leu Pro Asp
115 120 125Glu Ala Tyr Tyr Arg Glu Glu Ala His Ala Glu Thr Leu Ala Ala Tyr
130 135 140Lys Glu His Val Glu Arg Met Leu Gly Tyr Leu Asp Asn Ser Arg Leu145 150 155 160Phe Gly Leu Ser Ala Ala Ser Ala Ala Ala Arg Ile Val Ala Leu Glu
165 170 175Thr Glu Ile Ala Ala Gly His Trp Asp Val Val Lys Thr Arg Asp Ala
180 185 190Val Ala Thr Tyr Asn Pro Thr Glu Leu Gly Ala Leu Pro Pro Lys Val
195 200 205Arg Thr Leu Leu Ser Ser Ala Gly Leu Pro Asp Gln Arg Leu Val Ser
210 215 220Met Met Pro Ser Tyr Leu Asp His Leu Asn Gly Leu Leu Val Asp Asp225 230 235 240Arg Leu Pro Asp Trp Gln Leu Trp Ala Thr Trp His Ile Leu Arg Ser
245 250 255Arg Ala Gly Leu Leu Thr Glu Glu Ile Ser Gln Ala Asn Phe Asp Phe
260 265 270Tyr Gly Thr Lys Leu Ser Gly Ala Thr Glu Gln Lys Asp Arg Trp Lys
275 280 285Arg Ala Val Gly Leu Ala Glu Arg Met Val Gly Glu Glu Ile Gly Gln
290 295 300Arg Phe Val Glu Arg His Phe Pro Ala Ser Ser Lys Glu His Met Leu305 310 315 320Glu Leu Val Asp Tyr Leu Val Ala Ala Tyr Arg Asp Arg lle Ser Asn
325 330 335Leu Glu Trp Met Thr Pro Ala Thr Arg Glu Arg Ala Leu Glu Lys Leu
340 345 350Gly Lys Phe Asn Ala Lys Ile Gly Tyr Pro Asp Lys Trp Arg Ser Tyr
355 360 365Glu Gly Leu Glu Phe Gly Ser Asp Leu Val Asp Asn Ser Arg Lys Gly
370 375 380Ser Ala Phe Leu His Asp Tyr Glu Leu Gly Lys Ile Gly Lys Pro Ala385 390 395 400Asp Arg Asp Glu Trp Val Thr Thr Pro Gln Thr Val Asn Ala Phe Tyr
405 410 415Asn Pro Val Val Asn Asp Ile Thr Phe Pro Ala Ala Ile Leu Arg Ala
420 425 430Pro Phe Phe Asp Pro Glu Ala Glu Ala Ala Glu Asn Phe Gly Ala Ile
435 440 445Gly Ala Val Ile Gly His Glu Ile Gly His Gly Phe Asp Asp Gln Gly
450 455 460Ser Gln Tyr Asp Gly Asp Gly Asn Leu Asn Ser Trp Trp Thr Asp Glu465 470 475 480Asp Arg Ser Ala Phe Glu Gln Leu Thr Ser Arg Leu Val Thr Gln Phe
485 490 495Ser Gly Leu Val Pro Ala Val Leu Thr Ser Glu Gly Ile Asp Thr Asp
500 505 510Gly Val Asn Gly Glu Phe Thr Leu Gly Glu Asn Ile Gly Asp Leu Gly
515 520 525Gly Leu Gly Ile Ala Val Val Ala Tyr Glu Lys Tyr Leu Ala Asp Arg
530 535 540Gly Gln Thr Phe Glu Thr Ser Pro Val Gln Lys Phe Glu Ala Glu Gly545 550 555 560Ala Glu Glu Gly Leu Ala Glu Gln Glu Phe Asn Gly Leu Gln Arg Leu
565 570 575Phe Leu Ser Trp Ala Arg Val Trp Arg Thr Lys Ile Arg Pro Gln Met
580 585 590Ala Val Gln Tyr Leu Ala Ile Asp Pro His Ser Pro Ala Glu Phe Arg
595 600 605Cys Asn Val Ile Ala Gly Asn Val Ala Glu Phe Tyr Glu Ala Phe Asp
610 615 620Val Pro Glu Asp Ala Pro Val Tyr Ile Lys Pro Glu Glu Arg Leu Ala625 630 635 640Ile Trp<210>45<211>1734<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1711)<223>FRXA01868<400>45atgcggatct gaacaggctg tctgttgcta actcatcgtt ttcgcagctg ctctcggcga 60actggaccgt gaaggcgttg gcgcgccagt aggtttctgg gtg gag aag gat tct 115
Val Glu Lys Asp Ser
1 5tcc tcc aac gaa tcc gtc gcc tat gtc atc cag tcc ggc ctc ggc ctg 163Ser Ser Asn Glu Ser Val Ala Tyr Val Ile Gln Ser Gly Leu Gly Leu
10 15 20ccc gat gag gct tat tac cgc gag gag gca cac gcc gaa act ctc gcg 211Pro Asp Glu Ala Tyr Tyr Arg Glu Glu Ala His Ala Glu Thr Leu Ala
25 30 35gcc tac aaa gag cac gtt gag cgc atg ctc ggc tac ttg gat aac agc 259Ala Tyr Lys Glu His Val Glu Arg Met Leu Gly Tyr Leu Asp Asn Ser
40 45 50cgc ctc ttc ggt ctg tcg gct gct tcc gct gcc gca cga att gtc gcc 307Arg Leu Phe Gly Leu Ser Ala Ala Ser Ala Ala Ala Arg Ile Val Ala
55 60 65ctg gaa acg gaa atc gct gct ggc cac tgg gat gtc gtg aag acc cgc 355Leu Glu Thr Glu Ile Ala Ala Gly His Trp Asp Val Val Lys Thr Arg70 75 80 85gac gcc gta gcc acc tac aac ccc acc gaa ctc ggc gcg ctg cca cca 403Asp Ala Val Ala Thr Tyr Asn Pro Thr Glu Leu Gly Ala Leu Pro Pro
90 95 100aag gtc cgc acg ctg ctc agt tcc gca ggc ctc ccg gac cag cgc ctg 451Lys Val Arg Thr Leu Leu Ser Ser Ala Gly Leu Pro Asp Gln Arg Leu
105 110 115gta tcg atg atg ccg tca tac ctc gac cac ctc aac ggc ttg ctt gtc 499Val Ser Met Met Pro Ser Tyr Leu Asp His Leu Asn Gly Leu Leu Val
120 125 130gac gac cgc ctc ccc gat tgg cag cta tgg gca acc tgg cac atc ttg 547Asp Asp Arg Leu Pro Asp Trp Gln Leu Trp Ala Thr Trp His Ile Leu
135 140 145agg tct cga gca gga ctg ttg acc gag gaa att agc caa gca aac ttc 595Arg Ser Arg Ala Gly Leu Leu Thr Glu Glu Ile Ser Gln Ala Asn Phe150 155 160 165gac ttc tat ggc acc aaa ctg tcc ggc gcc acc gag caa aaa gat cga 643Asp Phe Tyr Gly Thr Lys Leu Ser Gly Ala Thr Glu Gln Lys Asp Arg
170 175 180tgg aag cgt gct gtc ggc ctg gca gag cgc atg gtg ggc gag gaa atc 691Trp Lys Arg Ala Val Gly Leu Ala Glu Arg Met Val Gly Glu Glu Ile
185 190 195ggg caa cga ttc gtc gaa agg cat ttt cct gca agc tcc aag gag cac 739Gly Gln Arg Phe Val Glu Arg His Phe Pro Ala Ser Ser Lys Glu His
200 205 210atg ctt gag ctc gtc gac tac ctg gtt gcc gcc tac cgt gat cgc att 787Met Leu Glu Leu Val Asp Tyr Leu Val Ala Ala Tyr Arg Asp Arg Ile
215 220 225tcc aac ctc gaa tgg atg acg ccc gcc acc cgc gag cgt gcc ctg gaa 835Ser Asn Leu Glu Trp Met Thr Pro Ala Thr Arg Glu Arg Ala Leu Glu230 235 240 245aag ttg ggc aaa ttc aac gcg aaa atc ggc tac ccc gac aag tgg cgc 883Lys Leu Gly Lys Phe Asn Ala Lys Ile Gly Tyr Pro Asp Lys Trp Arg
250 255 260tcc tac gaa ggc ctc gaa ttc ggc tcc gac ctg gtg gac aac tcc cgc 931Ser Tyr Glu Gly Leu Glu Phe Gly Ser Asp Leu Val Asp Asn Ser Arg
265 270 275aag ggc tcc gcg ttc ctc cat gac tat gag ctg ggc aag atc ggc aaa 979Lys Gly Ser Ala Phe Leu His Asp Tyr Glu Leu Gly Lys Ile Gly Lys
280 285 290cca gcc gac cgc gac gaa tgg gtc acc acc cca caa acc gtc aac gcc 1027Pro Ala Asp Arg Asp Glu Trp Val Thr Thr Pro Gln Thr Val Asn Ala
295 300 305ttc tac aac ccc gtg gtc aac gac atc acc ttc ccc gca gcc atc ctg 1075Phe Tyr Asn Pro Val Val Asn Asp Ile Thr Phe Pro Ala Ala Ile Leu310 315 320 325cgc gca cca ttc ttc gac ccc gaa gca gaa gcc gca gaa aac ttc ggt 1123Arg Ala Pro Phe Phe Asp Pro Glu Ala Glu Ala Ala Glu Asn Phe Gly
330 335 340gca atc ggt gct gtg atc gga cac gaa atc ggc cac ggc ttt gac gat 1171Ala Ile Gly Ala Val Ile Gly His Glu Ile Gly His Gly Phe Asp Asp
345 350 355caa ggc agc caa tac gac ggc gac ggc aac ctc aac tcc tgg tgg acc 1219Gln Gly Ser Gln Tyr Asp Gly Asp Gly Asn Leu Asn Ser Trp Trp Thr
360 365 370gac gaa gac cgc tcc gca ttc gag cag ctc acc tca cgt ctg gtc acc 1267Asp Glu Asp Arg Ser Ala Phe Glu Gln Leu Thr Ser Arg Leu Val Thr
375 380 385caa ttc agc gga ctc gtc cct gcc gtc ctg acc tct gaa gga atc gac 1315Gln Phe Ser Gly Leu Val Pro Ala Val Leu Thr Ser Glu Gly Ile Asp390 395 400 405acc gac ggc gtc aac ggt gaa ttc act ctc ggc gaa aac atc ggt gac 1363Thr Asp Gly Val Asn Gly Glu Phe Thr Leu Gly Glu Asn Ile Gly Asp
410 415 420ctc ggc gga ttg ggc atc gct gtc gtt gcc tac gaa aag tac ctc gca 1411Leu Gly Gly Leu Gly Ile Ala Val Val Ala Tyr Glu Lys Tyr Leu Ala
425 430 435gac cgt ggc caa acc ttt gaa acc tca cca gtc caa aaa ttc gaa gca 1459Asp Arg Gly Gln Thr Phe Glu Thr Ser Pro Val Gln Lys Phe Glu Ala
440 445 450gaa ggc gcc gag gaa ggc ctg gcc gag caa gaa ttc aac ggt ctc caa 1507Glu Gly Ala Glu Glu Gly Leu Ala Glu Gln Glu Phe Asn Gly Leu Gln
455 460 465cgc ctc ttc ctg tcc tgg gct cgc gtg tgg cgc acc aaa atc cgc cca 1555Arg Leu Phe Leu Ser Trp Ala Arg Val Trp Arg Thr Lys Ile Arg Pro470 475 480 485cag atg gcc gtc caa tac ctg gcc atc gac cca cac tcc cct gca gaa 1603Gln Met Ala Val Gln Tyr Leu Ala Ile Asp Pro His Ser Pro Ala Glu
490 495 500ttc cgc tgc aat gtc atc gcc gga aac gtc gct gaa ttc tac gaa gca 1651Phe Arg Cys Asn Val Ile Ala Gly Asn Val Ala Glu Phe Tyr Glu Ala
505 510 515ttc gac gtc ccc gaa gat gca cct gtg tac atc aag cca gaa gag cgc 1699Phe Asp Val Pro Glu Asp Ala Pro Val Tyr Ile Lys Pro Glu Glu Arg
520 525 530cta gct atc tgg tagttgttag ttggtattga aaa 1734Leu Ala Ile Trp
535<210>46<211>537<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>46Val Glu Lys Asp Ser Ser Ser Asn Glu Ser Val Ala Tyr Val Ile Gln1 5 10 15Ser Gly Leu Gly Leu Pro Asp Glu Ala Tyr Tyr Arg Glu Glu Ala His
20 25 30Ala Glu Thr Leu Ala Ala Tyr Lys Glu His Val Glu Arg Met Leu Gly
35 40 45Tyr Leu Asp Asn Ser Arg Leu Phe Gly Leu Ser Ala Ala Ser Ala Ala
50 55 60Ala Arg Ile Val Ala Leu Glu Thr Glu Ile Ala Ala Gly His Trp Asp65 70 75 80Val Val Lys Thr Arg Asp Ala Val Ala Thr Tyr Asn Pro Thr Glu Leu
85 90 95Gly Ala Leu Pro Pro Lys Val Arg Thr Leu Leu Ser Ser Ala Gly Leu
100 105 110Pro Asp Gln Arg Leu Val Ser Met Met Pro Ser Tyr Leu Asp His Leu
115 120 125Asn Gly Leu Leu Val Asp Asp Arg Leu Pro Asp Trp Gln Leu Trp Ala
130 135 140Thr Trp His Ile Leu Arg Ser Arg Ala Gly Leu Leu Thr Glu Glu Ile145 150 155 160Ser Gln Ala Asn Phe Asp Phe Tyr Gly Thr Lys Leu Ser Gly Ala Thr
165 170 175Glu Gln Lys Asp Arg Trp Lys Arg Ala Val Gly Leu Ala Glu Arg Met
180 185 190Val Gly Glu Glu Ile Gly Gln Arg Phe Val Glu Arg His Phe Pro Ala
195 200 205Ser Ser Lys Glu His Met Leu Glu Leu Val Asp Tyr Leu Val Ala Ala
210 215 220Tyr Arg Asp Arg Ile Ser Asn Leu Glu Trp Met Thr Pro Ala Thr Arg225 230 235 240Glu Arg Ala Leu Glu Lys Leu Gly Lys Phe Asn Ala Lys Ile Gly Tyr
245 250 255Pro Asp Lys Trp Arg Ser Tyr Glu Gly Leu Glu Phe Gly Ser Asp Leu
260 265 270Val Asp Asn Ser Arg Lys Gly Ser Ala Phe Leu His Asp Tyr Glu Leu
275 280 285Gly Lys Ile Gly Lys Pro Ala Asp Arg Asp Glu Trp Val Thr Thr Pro
290 295 300Gln Thr Val Asn Ala Phe Tyr Asn Pro Val Val Asn Asp Ile Thr Phe305 310 315 320Pro Ala Ala Ile Leu Arg Ala Pro Phe Phe Asp Pro Glu Ala Glu Ala
325 330 335Ala Glu Asn Phe Gly Ala Ile Gly Ala Val Ile Gly His Glu Ile Gly
340 345 350His Gly Phe Asp Asp Gln Gly Ser Gln Tyr Asp Gly Asp Gly Asn Leu
355 360 365Asn Ser Trp Trp Thr Asp Glu Asp Arg Ser Ala Phe Glu Gln Leu Thr
370 375 380Ser Arg Leu Val Thr Gln Phe Ser Gly Leu Val Pro Ala Val Leu Thr385 390 395 400Ser Glu Gly Ile Asp Thr Asp Gly Val Asn Gly Glu Phe Thr Leu Gly
405 410 415Glu Asn Ile Gly Asp Leu Gly Gly Leu Gly Ile Ala Val Val Ala Tyr
420 425 430Glu Lys Tyr Leu Ala Asp Arg Gly Gln Thr Phe Glu Thr Ser Pro Val
435 440 445Gln Lys Phe Glu Ala Glu Gly Ala Glu Glu Gly Leu Ala Glu Gln Glu
450 455 460Phe Asn Gly Leu Gln Arg Leu Phe Leu Ser Trp Ala Arg Val Trp Arg465 470 475 480Thr Lys Ile Arg Pro Gln Met Ala Val Gln Tyr Leu Ala Ile Asp Pro
485 490 495His Ser Pro Ala Glu Phe Arg Cys Asn Val Ile Ala Gly Asn Val Ala
500 505 510Glu Phe Tyr Glu Ala Phe Asp Val Pro Glu Asp Ala Pro Val Tyr Ile
515 520 525Lys Pro Glu Glu Arg Leu Ala Ile Trp
530 535<210>47<211>426<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(403)<223>FRXA01869<400>47tgacaggcta ccttctgggg tggacatgat ccccaacgct caacccactt gtggcaccaa 60ccacaaaccc tgtggcggta aatcccctag agtaggccac atg aag gat ctt tat 115
Met Lys Asp Leu Tyr
1 5cgc ttt gtc aat ggc ctg tgg ctt gac acc cac atc att ccc gac gat 163Arg Phe Val Asn Gly Leu Trp Leu Asp Thr His Ile Ile Pro Asp Asp
10 15 20cgc gcg gtg gac ggc acg ttc cac aag ctg cgc gat gat gct gaa gaa 211Arg Ala Val Asp Gly Thr Phe His Lys Leu Arg Asp Asp Ala Glu Glu
25 30 35gac gtc cat gag atc gtc aag gaa gac act gga cgc gca ggc aca ctt 259Asp Val His Glu Ile Val Lys Glu Asp Thr Gly Arg Ala Gly Thr Leu
40 45 50tat gcc tca ttt atg gat act gac gcc atc aac gct gct ggt gtt gca 307Tyr Ala Ser Phe Met Asp Thr Asp Ala Ile Asn Ala Ala Gly Val Ala
55 60 65ccg ctc gat gcg gat ctg aac agg ctg tct gtt gct aac tca tcg ttt 355Pro Leu Asp Ala Asp Leu Asn Arg Leu Ser Val Ala Asn Ser Ser Phe70 75 80 85tcg cag ctg ctc tcg gcg aac tgg acc gtg aag gcg ttg gcg cgc cag 403Ser Gln Leu Leu Ser Ala Asn Trp Thr Val Lys Ala Leu Ala Arg Gln
90 95 100taggtttctg ggtggagaag gat 426<210>48<211>101<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>48Met Lys Asp Leu Tyr Arg Phe Val Asn Gly Leu Trp Leu Asp Thr His1 5 10 15Ile Ile Pro Asp Asp Arg Ala Val Asp Gly Thr Phe His Lys Leu Arg
20 25 30Asp Asp Ala Glu Glu Asp Val His Glu Ile Val Lys Glu Asp Thr Gly
35 40 45Arg Ala Gly Thr Leu Tyr Ala Ser Phe Met Asp Thr Asp Ala Ile Asn
50 55 60Ala Ala Gly Val Ala Pro Leu Asp Ala Asp Leu Asn Arg Leu Ser Val65 70 75 80Ala Asn Ser Ser Phe Ser Gln Leu Leu Ser Ala Asn Trp Thr Val Lys
85 90 95Ala Leu Ala Arg Gln
100<210>49<211>2898<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(2875)<223>RXN03028<400>49ctaattccaa gccgagctga aaaagtctgg aagttttgcc caataagggc gttaaagtgg 60gtgaaagcga atttagaaat aaagaattaa ggggagagac atg ttc gag agg ttt 115
Met Phe Glu Arg Phe
1 5acc gat cgt gca cgc cgc gtg att gtg ctc gcg cag gaa gag gcg cgc 163Thr Asp Arg Ala Arg Arg Val Ile Val Leu Ala Gln Glu Glu Ala Arg
10 15 20atg ctc aac cac aat tac atc ggc acg gag cac att ctc ctc ggc ctc 211Met Leu Asn His Asn Tyr Ile Gly Thr Glu His Ile Leu Leu Gly Leu
25 30 35att cac gag ggc gag ggc gtt gca gcc aag gct ttg gaa tcc atg gga 259Ile His Glu Gly Glu Gly Val Ala Ala Lys Ala Leu Glu Ser Met Gly
40 45 50att tcc ctg gac gcc gtc cgc cag gaa gtc gaa gag att atc ggc cag 307Ile Ser Leu Asp Ala Val Arg Gln Glu Val Glu Glu Ile Ile Gly Gln
55 60 65ggc tcc cag ccc acc acc ggc cac att cct ttt act cca cgt gcc aag 355Gly Ser Gln Pro Thr Thr Gly His Ile Pro Phe Thr Pro Arg Ala Lys70 75 80 85aag gtc ctg gag ctc agc ctc cgc gaa ggc cta caa atg gga cac aag 403Lys Val Leu Glu Leu Ser Leu Arg Glu Gly Leu Gln Met Gly His Lys
90 95 100tac atc ggt act gag ttc ctg ctt ctc ggt ttg atc cgt gag ggc gag 451Tyr Ile Gly Thr Glu Phe Leu Leu Leu Gly Leu Ile Arg Glu Gly Glu
105 110 115ggc gtt gct gcc cag gtc ctg gtc aag ctt ggt gct gat ctg cca cgc 499Gly Val Ala Ala Gln Val Leu Val Lys Leu Gly Ala Asp Leu Pro Arg
120 125 130gtg cgt cag caa gtt att cag ctt ctc tcc ggc tac gaa ggt ggc cag 547Val Arg Gln Gln Val Ile Gln Leu Leu Ser Gly Tyr Glu Gly Gly Gln
135 140 145ggc gga tcc cca gag ggc ggc cag ggc gcc cct act ggc ggt gac gct 595Gly Gly Ser Pro Glu Gly Gly Gln Gly Ala Pro Thr Gly Gly Asp Ala150 155 160 165gtt ggt gca gga gct gct cct ggc ggt cgt cca tct tcg ggc agc cca 643Val Gly Ala Gly Ala Ala Pro Gly Gly Arg Pro Ser Ser Gly Ser Pro
170 175 180ggc gag cgt tct acc tct ttg gtc ctt gac cag ttc ggc cgc aac ctc 691Gly Glu Arg Ser Thr Ser Leu Val Leu Asp Gln Phe Gly Arg Asn Leu
185 190 195acc cag gct gca aag gac ggc aag ctg gat cca gtt gtt ggt cgc gat 739Thr Gln Ala Ala Lys Asp Gly Lys Leu Asp Pro Val Val Gly Arg Asp
200 205 210aag gaa atc gag cgc atc atg cag gtg ctc tcc cgt cgt acc aag aac 787Lys Glu Ile Glu Arg Ile Met Gln Val Leu Ser Arg Arg Thr Lys Asn
215 220 225aac cca gtt ctt att ggt gag cca ggt gtt ggt aag acc gca gtt gtt 835Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys Thr Ala Val Val230 235 240 245gaa ggt ctt gca cta gac att gtt aac ggc aag gtt cca gag acc ctc 883Glu Gly Leu Ala Leu Asp Ile Val Asn Gly Lys Val Pro Glu Thr Leu
250 255 260aag gac aag cag gtt tac tcc ctt gac tta ggt tcc ctg gtt gca ggt 931Lys Asp Lys Gln Val Tyr Ser Leu Asp Leu Gly Ser Leu Val Ala Gly
265 270 275tcc cgt tac cgc ggt gac ttc gaa gag cga ctg aag aag gtc ctc aag 979Ser Arg Tyr Arg Gly Asp Phe Glu Glu Arg Leu Lys Lys Val Leu Lys
280 285 290gag att aac cag cgc ggc gac atc atc ctg ttt atc gat gag atc cac 1027Glu Ile Asn Gln Arg Gly Asp Ile Ile Leu Phe Ile Asp Glu Ile His
295 300 305acc ctc gtg ggt gca ggt gca gca gaa ggc gca atc gac gct gcc tcc 1075Thr Leu Val Gly Ala Gly Ala Ala Glu Gly Ala Ile Asp Ala Ala Ser310 315 320 325ctg ctt aag cca aag ctt gcc cgc ggt gaa ctg cag acc att ggt gca 1123Leu Leu Lys Pro Lys Leu Ala Arg Gly Glu Leu Gln Thr Ile Gly Ala
330 335 340acc acc ctg gat gag tac cgt aag cac att gaa aag gac gca gct ctt 1171Thr Thr Leu Asp Glu Tyr Arg Lys His Ile Glu Lys Asp Ala Ala Leu
345 350 355gag cgt cgt ttc cag cca gtg cag gtt cca gag cct tcg gtt gat ctc 1219Glu Arg Arg Phe Gln Pro Val Gln Val Pro Glu Pro Ser Val Asp Leu
360 365 370acc gtt gag atc ttg aag ggt ctg cgc gac cgc tac gaa gct cac cac 1267Thr Val Glu Ile Leu Lys Gly Leu Arg Asp Arg Tyr Glu Ala His His
375 380 385cgc gta tcc atc acc gat ggt gct ctt act gca gca gct cag ctt gct 1315Arg Val Ser Ile Thr Asp Gly Ala Leu Thr Ala Ala Ala Gln Leu Ala390 395 400 405gat cgc tac atc aac gac cgc ttc ttg cca gat aag gcc gtt gac ctc 1363Asp Arg Tyr Ile Asn Asp Arg Phe Leu Pro Asp Lys Ala Val Asp Leu
410 415 420atc gat gag gct ggc gcc cgc atg cgc ate aag cgc atg acc gca cct 1411Ile Asp Glu Ala Gly Ala Arg Met Arg Ile Lys Arg Met Thr Ala Pro
425 430 435tcc tcc ctc cgc gag gtt gat gag cgt atc gct gat gtt cgc cgt gag 1459Ser Ser Leu Arg Glu Val Asp Glu Arg Ile Ala Asp Val Arg Arg Glu
440 445 450aag gaa gca gcg atc gat gct cag gac ttt gag aag gca gca ggt ctt 1507Lys Glu Ala Ala Ile Asp Ala Gln Asp Phe Glu Lys Ala Ala Gly Leu
455 460 465cgc gat aag gag cgc aag ctc ggc gaa gag cgt tca gag aag gaa aag 1555Arg Asp Lys Glu Arg Lys Leu Gly Glu Glu Arg Ser Glu Lys Glu Lys470 475 480 485cag tgg cgc tcc ggc gac ctc gag gac atc gct gag gtt ggc gaa gag 1603Gln Trp Arg Ser Gly Asp Leu Glu Asp Ile Ala Glu Val Gly Glu Glu
490 495 500cag atc gca gaa gta ctg gcc aac tgg act ggt att cct gtc ttc aag 1651Gln Ile Ala Glu Val Leu Ala Asn Trp Thr Gly Ile Pro Val Phe Lys
505 510 515ctc acc gaa gct gaa tct tca cgc ctg ctc aac atg gaa gaa gag ttg 1699Leu Thr Glu Ala Glu Ser Ser Arg Leu Leu Asn Met Glu Glu Glu Leu
520 525 530cac aag cgc atc atc gga cag gat gaa gct gtc aag gct gtc tcc cgt 1747His Lys Arg Ile Ile Gly Gln Asp Glu Ala Val Lys Ala Val Ser Arg
535 540 545gcg atc cgt cgt acc cgt gca ggt ctg aag gat cct aag cgt cct tcc 1795Ala Ile Arg Arg Thr Arg Ala Gly Leu Lys Asp Pro Lys Arg Pro Ser550 555 560 565ggc tcc ttc atc ttc gct ggt cca tcc ggc gtt ggt aag acc gag ctg 1843Gly Ser Phe Ile Phe Ala Gly Pro Ser Gly Val Gly Lys Thr Glu Leu
570 575 580tcc aag gct ctc gca gga ttc ctc ttc ggt gac gat gat tcc ctc atc 1891Ser Lys Ala Leu Ala Gly Phe Leu Phe Gly Asp Asp Asp Ser Leu Ile
585 590 595caa atc gac atg ggt gag ttc cac gac cgc ttc acc gcg tcc cga ctt 1939Gln Ile Asp Met Gly Glu Phe His Asp Arg Phe Thr Ala Ser Arg Leu
600 605 610ttc ggt gcc cct ccg gga tac gtt ggc tac gaa gaa ggt ggc cag ctg 1987Phe Gly Ala Pro Pro Gly Tyr Val Gly Tyr Glu Glu Gly Gly Gln Leu
615 620 625acc gag aag gtt cgc cgt aag cca ttc tcc gtt gtg ctt ttc gac gaa 2035Thr Glu Lys Val Arg Arg Lys Pro Phe Ser Val Val Leu Phe Asp Glu630 635 640 645atc gag aag gcc cac aag gag atc tac aac acc ttg ctg cag gtg ttg 2083Ile Glu Lys Ala His Lys Glu Ile Tyr Asn Thr Leu Leu Gln Val Leu
650 655 660gaa gat ggt cgc ctt acc gat ggt cag gga cgc atc gtg gac ttc aag 2131Glu Asp Gly Arg Leu Thr Asp Gly Gln Gly Arg Ile Val Asp Phe Lys
665 670 675aac acc gtc ctg atc ttc acc tcc aac ctg ggc acc gct gac atc tcc 2179Asn Thr Val Leu Ile Phe Thr Ser Asn Leu Gly Thr Ala Asp Ile Ser
680 685 690aag gct gtt ggc ctg ggc ttc tcc gga tcc tcc gag act gac agc gat 2227Lys Ala Val Gly Leu Gly Phe Ser Gly Ser Ser Glu Thr Asp Ser Asp
695 700 705gct cag tac gac cgc atg aag aac aag gtc cac gac gag ctg aag aag 2275Ala Gln Tyr Asp Arg Met Lys Asn Lys Val His Asp Glu Leu Lys Lys710 715 720 725cac ttc cgc cct gag ttc ctg aac cgt att gat gag atc gtg gtc ttc 2323His Phe Arg Pro Glu Phe Leu Asn Arg Ile Asp Glu Ile Val Val Phe
730 735 740cac cag ctc acc aag gat cag atc gtt cag atg gtc gac ctt ctt atc 2371His Gln Leu Thr Lys Asp Gln Ile Val Gln Met Val Asp Leu Leu Ile
745 750 755ggt cgc gtt tcc aac gca ctg gct gag aag gac atg agc atc gaa ctg 2419Gly Arg Val Ser Asn Ala Leu Ala Glu Lys Asp Met Ser Ile Glu Leu
760 765 770act gag aag gcc aag gac ctc ctg gct aac cga ggc ttc gat cca gtt 2467Thr Glu Lys Ala Lys Asp Leu Leu Ala Asn Arg Gly Phe Asp Pro Val
775 780 785ctg ggt gca cga cca ttg cgt cgc acc atc cag cgc gaa att gaa gac 2515Leu Gly Ala Arg Pro Leu Arg Arg Thr Ile Gln Arg Glu Ile Glu Asp790 795 800 805cag atg tcc gag aag atc ctc ttc ggt gaa atc ggc gca ggc gag atc 2563Gln Met Ser Glu Lys Ile Leu Phe Gly Glu Ile Gly Ala Gly Glu Ile
810 815 820gtc acc gtt gac gtc gaa ggc tgg gac ggc gag tcc aag gac acc gac 2611Val Thr Val Asp Val Glu Gly Trp Asp Gly Glu Ser Lys Asp Thr Asp
825 830 835cgt gcg aag ttc acc ttc aca cca cgt cca aag cca atg cca gaa ggt 2659Arg Ala Lys Phe Thr Phe Thr Pro Arg Pro Lys Pro Met Pro Glu Gly
840 845 850aag ttc tct gag atc tct gtc gag gct gcg gaa gca att caa gat gta 2707Lys Phe Ser Glu Ile Ser Val Glu Ala Ala Glu Ala Ile Gln Asp Val
855 860 865gat tct gca gct gac ggc gat gtc cca gaa acc gat tca ctt tcc gac 2755Asp Ser Ala Ala Asp Gly Asp Val Pro Glu Thr Asp Ser Leu Ser Asp870 875 880 885att gac ctt gaa acc ctt gaa aag ttt gag gaa gat gta gaa aac ggc 2803Ile Asp Leu Glu Thr Leu Glu Lys Phe Glu Glu Asp Val Glu Asn Gly
890 895 900acc gac att gat cag gtg tcc ggt gac tac tac ggc acc gat gat cag 2851Thr Asp Ile Asp Gln Val Ser Gly Asp Tyr Tyr Gly Thr Asp Asp Gln
905 910 915gga ggc act gct cca agc aag gag tagcaacctt ttgaaaaagg gcc 2898Gly Gly Thr Ala Pro Ser Lys Glu
920 925<210>50<211>925<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>50Met Phe Glu Arg Phe Thr Asp Arg Ala Arg Arg Val Ile Val Leu Ala1 5 10 15Gln Glu Glu Ala Arg Met Leu Asn His Asn Tyr Ile Gly Thr Glu His
20 25 30Ile Leu Leu Gly Leu Ile His Glu Gly Glu Gly Val Ala Ala Lys Ala
35 40 45Leu Glu Ser Met Gly Ile Ser Leu Asp Ala Val Arg Gln Glu Val Glu
50 55 60Glu Ile Ile Gly Gln Gly Ser Gln Pro Thr Thr Gly His Ile Pro Phe65 70 75 80Thr Pro Arg Ala Lys Lys Val Leu Glu Leu Ser Leu Arg Glu Gly Leu
85 90 95Gln Met Gly His Lys Tyr Ile Gly Thr Glu Phe Leu Leu Leu Gly Leu
100 105 110Ile Arg Glu Gly Glu Gly Val Ala Ala Gln Val Leu Val Lys Leu Gly
115 120 125Ala Asp Leu Pro Arg Val Arg Gln Gln Val Ile Gln Leu Leu Ser Gly
130 135 140Tyr Glu Gly Gly Gln Gly Gly Ser Pro Glu Gly Gly Gln Gly Ala Pro145 150 155 160Thr Gly Gly Asp Ala Val Gly Ala Gly Ala Ala Pro Gly Gly Arg Pro
165 170 175Ser Ser Gly Ser Pro Gly Glu Arg Ser Thr Ser Leu Val Leu Asp Gln
180 185 190Phe Gly Arg Asn Leu Thr Gln Ala Ala Lys Asp Gly Lys Leu Asp Pro
195 200 205Val Val Gly Arg Asp Lys Glu Ile Glu Arg Ile Met Gln Val Leu Ser
210 215 220Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly225 230 235 240Lys Thr Ala Val Val Glu Gly Leu Ala Leu Asp Ile Val Asn Gly Lys
245 250 255Val Pro Glu Thr Leu Lys Asp Lys Gln Val Tyr Ser Leu Asp Leu Gly
260 265 270Ser Leu Val Ala Gly Ser Arg Tyr Arg Gly Asp Phe Glu Glu Arg Leu
275 280 285Lys Lys Val Leu Lys Glu Ile Asn Gln Arg Gly Asp Ile Ile Leu Phe
290 295 300Ile Asp Glu lle His Thr Leu Val Gly Ala Gly Ala Ala Glu Gly Ala305 310 315 320Ile Asp Ala Ala Ser Leu Leu Lys Pro Lys Leu Ala Arg Gly Glu Leu
325 330 335Gln Thr Ile Gly Ala Thr Thr Leu Asp Glu Tyr Arg Lys His Ile Glu
340 345 350Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Pro Val Gln Val Pro Glu
355 360 365Pro Ser Val Asp Leu Thr Val Glu Ile Leu Lys Gly Leu Arg Asp Arg
370 375 380Tyr Glu Ala His His Arg Val Ser Ile Thr Asp Gly Ala Leu Thr Ala385 390 395 400Ala Ala Gln Leu Ala Asp Arg Tyr Ile Asn Asp Arg Phe Leu Pro Asp
405 410 415Lys Ala Val Asp Leu Ile Asp Glu Ala Gly Ala Arg Met Arg Ile Lys
420 425 430Arg Met Thr Ala Pro Ser Ser Leu Arg Glu Val Asp Glu Arg Ile Ala
435 440 445Asp Val Arg Arg Glu Lys Glu Ala Ala Ile Asp Ala Gln Asp Phe Glu
450 455 460Lys Ala Ala Gly Leu Arg Asp Lys Glu Arg Lys Leu Gly Glu Glu Arg465 470 475 480Ser Glu Lys Glu Lys Gln Trp Arg Ser Gly Asp Leu Glu Asp Ile Ala
485 490 495Glu Val Gly Glu Glu Gln Ile Ala Glu Val Leu Ala Asn Trp Thr Gly
500 505 510Ile Pro Val Phe Lys Leu Thr Glu Ala Glu Ser Ser Arg Leu Leu Asn
515 520 525Met Glu Glu Glu Leu His Lys Arg Ile Ile Gly Gln Asp Glu Ala Val
530 535 540Lys Ala Val Ser Arg Ala Ile Arg Arg Thr Arg Ala Gly Leu Lys Asp545 550 555 560Pro Lys Arg Pro Ser Gly Ser Phe Ile Phe Ala Gly Pro Ser Gly Val
565 570 575Gly Lys Thr Glu Leu Ser Lys Ala Leu Ala Gly Phe Leu Phe Gly Asp
580 585 590Asp Asp Ser Leu Ile Gln Ile Asp Met Gly Glu Phe His Asp Arg Phe
595 600 605Thr Ala Ser Arg Leu Phe Gly Ala Pro Pro Gly Tyr Val Gly Tyr Glu
610 615 620Glu Gly Gly Gln Leu Thr Glu Lys Val Arg Arg Lys Pro Phe Ser Val625 630 635 640Val Leu Phe Asp Glu Ile Glu Lys Ala His Lys Glu Ile Tyr Asn Thr
645 650 655Leu Leu Gln Val Leu Glu Asp Gly Arg Leu Thr Asp Gly Gln Gly Arg
660 665 670Ile Val Asp Phe Lys Asn Thr Val Leu Ile Phe Thr Ser Asn Leu Gly
675 680 685Thr Ala Asp Ile Ser Lys Ala Val Gly Leu Gly Phe Ser Gly Ser Ser
690 695 700Glu Thr Asp Ser Asp Ala Gln Tyr Asp Arg Met Lys Asn Lys Val His705 710 715 720Asp Glu Leu Lys Lys His Phe Arg Pro Glu Phe Leu Asn Arg Ile Asp
725 730 735Glu Ile Val Val Phe His Gln Leu Thr Lys Asp Gln Ile Val Gln Met
740 745 750Val Asp Leu Leu Ile Gly Arg Val Ser Asn Ala Leu Ala Glu Lys Asp
755 760 765Met Ser Ile Glu Leu Thr Glu Lys Ala Lys Asp Leu Leu Ala Asn Arg
770 775 780Gly Phe Asp Pro Val Leu Gly Ala Arg Pro Leu Arg Arg Thr Ile Gln785 790 795 800Arg Glu Ile Glu Asp Gln Met Ser Glu Lys Ile Leu Phe Gly Glu Ile
805 810 815Gly Ala Gly Glu Ile Val Thr Val Asp Val Glu Gly Trp Asp Gly Glu
820 825 830Ser Lys Asp Thr Asp Arg Ala Lys Phe Thr Phe Thr Pro Arg Pro Lys
835 840 845Pro Met Pro Glu Gly Lys Phe Ser Glu Ile Ser Val Glu Ala Ala Glu
850 855 860Ala Ile Gln Asp Val Asp Ser Ala Ala Asp Gly Asp Val Pro Glu Thr865 870 875 880Asp Ser Leu Ser Asp Ile Asp Leu Glu Thr Leu Glu Lys Phe Glu Glu
885 890 895Asp Val Glu Asn Gly Thr Asp Ile Asp Gln Val Ser Gly Asp Tyr Tyr
900 905 910Gly Thr Asp Asp Gln Gly Gly Thr Ala Pro Ser Lys Glu
915 920 925<210>51<211>1104<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1081)<223>FRXA02470<400> 51ctaattccaa gccgagctga aaaagtctgg aagttttgcc caataagggc gttaaagtgg 60gtgaaagcga atttagaaat aaagaattaa ggggagagac atg ttc gag agg ttt 115
Met Phe Glu Arg Phe
1 5acc gat cgt gca cgc cgc gtg att gtg ctc gcg cag gaa gag gcg cgc 163Thr Asp Arg Ala Arg Arg Val Ile Val Leu Ala Gln Glu Glu Ala Arg
10 15 20atg ctc aac cac aat tac atc ggc acg gag cac att ctc ctc ggc ctc 211Met Leu Asn His Asn Tyr Ile Gly Thr Glu His Ile Leu Leu Gly Leu
25 30 35att cac gag ggc gag ggc gtt gca gcc aag gct ttg gaa tcc atg gga 259Ile His Glu Gly Glu Gly Val Ala Ala Lys Ala Leu Glu Ser Met Gly
40 45 50att tcc ctg gac gcc gtc cgc cag gaa gtc gaa gag att atc ggc cag 307Ile Ser Leu Asp Ala Val Arg Gln Glu Val Glu Glu Ile Ile Gly Gln
55 60 65ggc tcc cag ccc acc acc ggc cac att cct ttt act cca cgt gcc aag 355Gly Ser Gln Pro Thr Thr Gly His Ile Pro Phe Thr Pro Arg Ala Lys70 75 80 85aag gtc ctg gag ctc agc ctc cgc gaa ggc cta caa atg gga cac aag 403Lys Val Leu Glu Leu Ser Leu Arg Glu Gly Leu Gln Met Gly His Lys
90 95 100tac atc ggt act gag ttc ctg ctt ctc ggt ttg atc cgt gag ggc gag 451Tyr Ile Gly Thr Glu Phe Leu Leu Leu Gly Leu Ile Arg Glu Gly Glu
105 110 115ggc gtt gct gcc cag gtc ctg gtc aag ctt ggt gct gat ctg cca cgc 499Gly Val Ala Ala Gln Val Leu Val Lys Leu Gly Ala Asp Leu Pro Arg
120 125 130gtg cgt cag caa gtt att cag ctt ctc tcc ggc tac gaa ggt ggc cag 547Val Arg Gln Gln Val Ile Gln Leu Leu Ser Gly Tyr Glu Gly Gly Gln
135 140 145ggc gga tcc cca gag ggc ggc cag ggc gcc cct act ggc ggt gac gct 595Gly Gly Ser Pro Glu Gly Gly Gln Gly Ala Pro Thr Gly Gly Asp Ala150 155 160 165gtt ggt gca gga gct gct cct ggc ggt cgt cca tct tcg ggc agc cca 643Val Gly Ala Gly Ala Ala Pro Gly Gly Arg Pro Ser Ser Gly Ser Pro
170 175 180ggc gag cgt tct acc tct ttg gtc ctt gac cag ttc ggc cgc aac ctc 691Gly Glu Arg Ser Thr Ser Leu Val Leu Asp Gln Phe Gly Arg Asn Leu
185 190 195acc cag gct gca aag gac ggc aag ctg gat cca gtt gtt ggt cgc gat 739Thr Gln Ala Ala Lys Asp Gly Lys Leu Asp Pro Val Val Gly Arg Asp
200 205 210aag gaa atc gag cgc atc atg cag gtg ctc tcc cgt cgt acc aag aac 787Lys Glu Ile Glu Arg Ile Met Gln Val Leu Ser Arg Arg Thr Lys Asn
215 220 225aac cca gtt ctt att ggt gag cca ggt gtt ggt aag acc gca gtt gtt 835Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys Thr Ala Val Val230 235 240 245gaa ggt ctt gca cta gac att gtt aac ggc aag gtt cca gag acc ctc 883Glu Gly Leu Ala Leu Asp Ile Val Asn Gly Lys Val Pro Glu Thr Leu
250 255 260aag gac aag cag gtt tac tcc ctt gac tta ggt tcc ctg gtt gca ggt 931Lys Asp Lys Gln Val Tyr Ser Leu Asp Leu Gly Ser Leu Val Ala Gly
265 270 275tcc cgt tac cgc ggt gac ttc gaa gag cga ctg aag aag gtc ctc aag 979Ser Arg Tyr Arg Gly Asp Phe Glu Glu Arg Leu Lys Lys Val Leu Lys
280 285 290gag att aac cag cgc ggc gac atc atc ctg ttt atc gat gag atc cac 1027Glu Ile Asn Gln Arg Gly Asp Ile Ile Leu Phe Ile Asp Glu Ile His
295 300 305acc ctc gtg ggt gca ggt gca gca cga agg cgc aat cga cgc tgc ctc 1075Thr Leu Val Gly Ala Gly Ala Ala Arg Arg Arg Asn Arg Arg Cys Leu310 315 320 325cct gct taagccaaag cttgcccgcg gtg 1104Pro Ala<210>52<211>327<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>52Met Phe Glu Arg Phe Thr Asp Arg Ala Arg Arg Val Ile Val Leu Ala1 5 10 15Gln Glu Glu Ala Arg Met Leu Asn His Asn Tyr Ile Gly Thr Glu His
20 25 30Ile Leu Leu Gly Leu Ile His Glu Gly Glu Gly Val Ala Ala Lys Ala
35 40 45Leu Glu Ser Met Gly Ile Ser Leu Asp Ala Val Arg Gln Glu Val Glu
50 55 60Glu Ile Ile Gly Gln Gly Ser Gln Pro Thr Thr Gly His Ile Pro Phe65 70 75 80Thr Pro Arg Ala Lys Lys Val Leu Glu Leu Ser Leu Arg Glu Gly Leu
85 90 95Gln Met Gly His Lys Tyr Ile Gly Thr Glu Phe Leu Leu Leu Gly Leu
100 105 110Ile Arg Glu Gly Glu Gly Val Ala Ala Gln Val Leu Val Lys Leu Gly
115 120 125Ala Asp Leu Pro Arg Val Arg Gln Gln Val Ile Gln Leu Leu Ser Gly
130 135 140Tyr Glu Gly Gly Gln Gly Gly Ser Pro Glu Gly Gly Gln Gly Ala Pro145 150 155 160Thr Gly Gly Asp Ala Val Gly Ala Gly Ala Ala Pro Gly Gly Arg Pro
165 170 175Ser Ser Gly Ser Pro Gly Glu Arg Ser Thr Ser Leu Val Leu Asp Gln
180 185 190Phe Gly Arg Asn Leu Thr Gln Ala Ala Lys Asp Gly Lys Leu Asp Pro
195 200 205Val Val Gly Arg Asp Lys Glu Ile Glu Arg Ile Met Gln Val Leu Ser
210 215 220Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly225 230 235 240Lys Thr Ala Val Val Glu Gly Leu Ala Leu Asp Ile Val Asn Gly Lys
245 250 255Val Pro Glu Thr Leu Lys Asp Lys Gln Val Tyr Ser Leu Asp Leu Gly
260 265 270Ser Leu Val Ala Gly Ser Arg Tyr Arg Gly Asp Phe Glu Glu Arg Leu
275 280 285Lys Lys Val Leu Lys Glu Ile Asn Gln Arg Gly Asp Ile Ile Leu Phe
290 295 300Ile Asp Glu Ile His Thr Leu Val Gly Ala Gly Ala Ala Arg Arg Arg305 310 315 320Asn Arg Arg Cys Leu Pro Ala
325<210>53<211>1956<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1933)<223>FRXA02471<400>53ggtgacttcg aagagcgact gaagaaggtc ctcaaggaga ttaaccagcg cggcgacatc 60atcctgttta tcgatgagat ccacaccctc gtgggtgcag gtg cag cac gaa ggc 115
Val Gln His Glu Gly
1 5gca atc gac gct gcc tcc ctg ctt aag cca aag ctt gcc cgc ggt gaa 163Ala Ile Asp Ala Ala Ser Leu Leu Lys Pro Lys Leu Ala Arg Gly Glu
10 15 20ctg cag acc att ggt gca acc acc ctg gat gag tac cgt aag cac att 211Leu Gln Thr Ile Gly Ala Thr Thr Leu Asp Glu Tyr Arg Lys His Ile
25 30 35gaa aag gac gca gct ctt gag cgt cgt ttc cag cca gtg cag gtt cca 259Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Pro Val Gln Val Pro
40 45 50gag cct tcg gtt gat ctc acc gtt gag atc ttg aag ggt ctg cgc gac 307Glu Pro Ser Val Asp Leu Thr Val Glu Ile Leu Lys Gly Leu Arg Asp
55 60 65cgc tac gaa gct cac cac cgc gta tcc atc acc gat ggt gct ctt act 355Arg Tyr Glu Ala His His Arg Val Ser Ile Thr Asp Gly Ala Leu Thr70 75 80 85gca gca gct cag ctt gct gat cgc tac atc aac gac cgc ttc ttg cca 403Ala Ala Ala Gln Leu Ala Asp Arg Tyr Ile Asn Asp Arg Phe Leu Pro
90 95 100gat aag gcc gtt gac ctc atc gat gag gct ggc gcc cgc atg cgc atc 451Asp Lys Ala Val Asp Leu Ile Asp Glu Ala Gly Ala Arg Met Arg Ile
105 110 115aag cgc atg acc gca cct tcc tcc ctc cgc gag gtt gat gag cgt atc 499Lys Arg Met Thr Ala Pro Ser Ser Leu Arg Glu Val Asp Glu Arg Ile
120 125 130gct gat gtt cgc cgt gag aag gaa gca gcg atc gat gct cag gac ttt 547Ala Asp Val Arg Arg Glu Lys Glu Ala Ala Ile Asp Ala Gln Asp Phe
135 140 145gag aag gca gca ggt ctt cgc gat aag gag cgc aag ctc ggc gaa gag 595Glu Lys Ala Ala Gly Leu Arg Asp Lys Glu Arg Lys Leu Gly Glu Glu150 155 160 165cgt tca gag aag gaa aag cag tgg cgc tcc ggc gac ctc gag gac atc 643Arg Ser Glu Lys Glu Lys Gln Trp Arg Ser Gly Asp Leu Glu Asp Ile
170 175 180gct gag gtt ggc gaa gag cag atc gca gaa gta ctg gcc aac tgg act 691Ala Glu Val Gly Glu Glu Gln Ile Ala Glu Val Leu Ala Asn Trp Thr
185 190 195ggt att cct gtc ttc aag ctc acc gaa gct gaa tct tca cgc ctg ctc 739Gly Ile Pro Val Phe Lys Leu Thr Glu Ala Glu Ser Ser Arg Leu Leu
200 205 210aac atg gaa gaa gag ttg cac aag cgc atc atc gga cag gat gaa gct 787Asn Met Glu Glu Glu Leu His Lys Arg Ile Ile Gly Gln Asp Glu Ala
215 220 225gtc aag gct gtc tcc cgt gcg atc cgt cgt acc cgt gca ggt ctg aag 835Val Lys Ala Val Ser Arg Ala Ile Arg Arg Thr Arg Ala Gly Leu Lys230 235 240 245gat cct aag cgt cct tcc ggc tcc ttc atc ttc gct ggt cca tcc ggc 883Asp Pro Lys Arg Pro Ser Gly Ser Phe Ile Phe Ala Gly Pro Ser Gly
250 255 260gtt ggt aag acc gag ctg tcc aag gct ctc gca gga ttc ctc ttc ggt 931Val Gly Lys Thr Glu Leu Ser Lys Ala Leu Ala Gly Phe Leu Phe Gly
265 270 275gac gat gat tcc ctc atc caa atc gac atg ggt gag ttc cac gac cgc 979Asp Asp Asp Ser Leu Ile Gln Ile Asp Met Gly Glu Phe His Asp Arg
280 285 290ttc acc gcg tcc cga ctt ttc ggt gcc cct ccg gga tac gtt ggc tac 1027Phe Thr Ala Ser Arg Leu Phe Gly Ala Pro Pro Gly Tyr Val Gly Tyr
295 300 305gaa gaa ggt ggc cag ctg acc gag aag gtt cgc cgt aag cca ttc tcc 1075Glu Glu Gly Gly Gln Leu Thr Glu Lys Val Arg Arg Lys Pro Phe Ser310 315 320 325gtt gtg ctt ttc gac gaa atc gag aag gcc cac aag gag atc tac aac 1123Val Val Leu Phe Asp Glu Ile Glu Lys Ala His Lys Glu Ile Tyr Asn
330 335 340acc ttg ctg cag gtg ttg gaa gat ggt cgc ctt acc gat ggt cag gga 1171Thr Leu Leu Gln Val Leu Glu Asp Gly Arg Leu Thr Asp Gly Gln Gly
345 350 355cgc atc gtg gac ttc aag aac acc gtc ctg atc ttc acc tcc aac ctg 1219Arg Ile Val Asp Phe Lys Asn Thr Val Leu Ile Phe Thr Ser Asn Leu
360 365 370ggc acc gct gac atc tcc aag gct gtt ggc ctg ggc ttc tcc gga tcc 1267Gly Thr Ala Asp Ile Ser Lys Ala Val Gly Leu Gly Phe Ser Gly Ser
375 380 385tcc gag act gac agc gat gct cag tac gac cgc atg aag aac aag gtc 1315Ser Glu Thr Asp Ser Asp Ala Gln Tyr Asp Arg Met Lys Asn Lys Val390 395 400 405cac gac gag ctg aag aag cac ttc cgc cct gag ttc ctg aac cgt att 1363His Asp Glu Leu Lys Lys His Phe Arg Pro Glu Phe Leu Asn Arg Ile
410 415 420gat gag atc gtg gtc ttc cac cag ctc acc aag gat cag atc gtt cag 1411Asp Glu Ile Val Val Phe His Gln Leu Thr Lys Asp Gln Ile Val Gln
425 430 435atg gtc gac ctt ctt atc ggt cgc gtt tcc aac gca ctg gct gag aag 1459Met Val Asp Leu Leu Ile Gly Arg Val Ser Asn Ala Leu Ala Glu Lys
440 445 450gac atg agc atc gaa ctg act gag aag gcc aag gac ctc ctg gct aac 1507Asp Met Ser Ile Glu Leu Thr Glu Lys Ala Lys Asp Leu Leu Ala Asn
455 460 465cga ggc ttc gat cca gtt ctg ggt gca cga cca ttg cgt cgc acc atc 1555Arg Gly Phe Asp Pro Val Leu Gly Ala Arg Pro Leu Arg Arg Thr Ile470 475 480 485cag cgc gaa att gaa gac cag atg tcc gag aag atc ctc ttc ggt gaa 1603Gln Arg Glu Ile Glu Asp Gln Met Ser Glu Lys Ile Leu Phe Gly Glu
490 495 500atc ggc gca ggc gag atc gtc acc gtt gac gtc gaa ggc tgg gac ggc 1651Ile Gly Ala Gly Glu Ile Val Thr Val Asp Val Glu Gly Trp Asp Gly
505 510 515gag tcc aag gac acc gac cgt gcg aag ttc acc ttc aca cca cgt cca 1699Glu Ser Lys Asp Thr Asp Arg Ala Lys Phe Thr Phe Thr Pro Arg Pro
520 525 530aag cca atg cca gaa ggt aag ttc tct gag atc tct gtc gag gct gcg 1747Lys Pro Met Pro Glu Gly Lys Phe Ser Glu Ile Ser Val Glu Ala Ala
535 540 545gaa gca att caa gat gta gat tct gca gct gac ggc gat gtc cca gaa 1795Glu Ala Ile Gln Asp Val Asp Ser Ala Ala Asp Gly Asp Val Pro Glu550 555 560 565acc gat tca ctt tcc gac att gac ctt gaa acc ctt gaa aag ttt gag 1843Thr Asp Ser Leu Ser Asp Ile Asp Leu Glu Thr Leu Glu Lys Phe Glu
570 575 580gaa gat gta gaa aac ggc acc gac att gat cag gtg tcc ggt gac tac 1891Glu Asp Val Glu Asn Gly Thr Asp Ile Asp Gln Val Ser Gly Asp Tyr
585 590 595tac ggc acc gat gat cag gga ggc act gct cca agc aag gag 1933Tyr Gly Thr Asp Asp Gln Gly Gly ThrAla Pro Ser Lys Glu
600 605 610tagcaacctt ttgaaaaagg gcc 1956<210>54<211>611<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400> 54Val Gln His Glu Gly Ala Ile Asp Ala Ala Ser Leu Leu Lys Pro Lys1 5 10 15Leu Ala Arg Gly Glu Leu Gln Thr Ile Gly Ala Thr Thr Leu Asp Glu
20 25 30Tyr Arg Lys His Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln
35 40 45Pro Val Gln Val Pro Glu Pro Ser Val Asp Leu Thr Val Glu Ile Leu
50 55 60Lys Gly Leu Arg Asp Arg Tyr Glu Ala His His Arg Val Ser Ile Thr65 70 75 80Asp Gly Ala Leu Thr Ala Ala Ala Gln Leu Ala Asp Arg Tyr Ile Asn
85 90 95Asp Arg Phe Leu Pro Asp Lys Ala Val Asp Leu Ile Asp Glu Ala Gly
100 105 110Ala Arg Met Arg Ile Lys Arg Met Thr Ala Pro Ser Ser Leu Arg Glu
115 120 125Val Asp Glu Arg Ile Ala Asp Val Arg Arg Glu Lys Glu Ala Ala Ile
130 135 140Asp Ala Gln Asp Phe Glu Lys Ala Ala Gly Leu Arg Asp Lys Glu Arg145 150 155 160Lys Leu Gly Glu Glu Arg Ser Glu Lys Glu Lys Gln Trp Arg Ser Gly
165 170 175Asp Leu Glu Asp Ile Ala Glu Val Gly Glu Glu Gln Ile Ala Glu Val
180 185 190Leu Ala Asn Trp Thr Gly Ile Pro Val Phe Lys Leu Thr Glu Ala Glu
195 200 205Ser Ser Arg Leu Leu Asn Met Glu Glu Glu Leu His Lys Arg Ile Ile
210 215 220Gly Gln Asp Glu Ala Val Lys Ala Val Ser Arg Ala Ile Arg Arg Thr225 230 235 240Arg Ala Gly Leu Lys Asp Pro Lys Arg Pro Ser Gly Ser Phe Ile Phe
245 250 255Ala Gly Pro Ser Gly Val Gly Lys Thr Glu Leu Ser Lys Ala Leu Ala
260 265 270Gly Phe Leu Phe Gly Asp Asp Asp Ser Leu Ile Gln Ile Asp Met Gly
275 280 285Glu Phe His Asp Arg Phe Thr Ala Ser Arg Leu Phe Gly Ala Pro Pro
290 295 300Gly Tyr Val Gly Tyr Glu Glu Gly Gly Gln Leu Thr Glu Lys Val Arg305 310 315 320Arg Lys Pro Phe Ser Val Val Leu Phe Asp Glu Ile Glu Lys Ala His
325 330 335Lys Glu Ile Tyr Asn Thr Leu Leu Gln Val Leu Glu Asp Gly Arg Leu
340 345 350Thr Asp Gly Gln Gly Arg Ile Val Asp Phe Lys Asn Thr Val Leu Ile
355 360 365Phe Thr Ser Asn Leu Gly Thr Ala Asp Ile Ser Lys Ala Val Gly Leu
370 375 380Gly Phe Ser Gly Ser Ser Glu Thr Asp Ser Asp Ala Gln Tyr Asp Arg385 390 395 400Met Lys Asn Lys Val His Asp Glu Leu Lys Lys His Phe Arg Pro Glu
405 410 415Phe Leu Asn Arg Ile Asp Glu Ile Val Val Phe His Gln Leu Thr Lys
420 425 430Asp Gln Ile Val Gln Met Val Asp Leu Leu Ile Gly Arg Val Ser Asn
435 440 445Ala Leu Ala Glu Lys Asp Met Ser Ile Glu Leu Thr Glu Lys Ala Lys
450 455 460Asp Leu Leu Ala Asn Arg Gly Phe Asp Pro Val Leu Gly Ala Arg Pro465 470 475 480Leu Arg Arg Thr Ile Gln Arg Glu Ile Glu Asp Gln Met Ser Glu Lys
485 490 495Ile Leu Phe Gly Glu Ile Gly Ala Gly Glu Ile Val Thr Val Asp Val
500 505 510Glu Gly Trp Asp Gly Glu Ser Lys Asp Thr Asp Arg Ala Lys Phe Thr
515 520 525Phe Thr Pro Arg Pro Lys Pro Met Pro Glu Gly Lys Phe Ser Glu Ile
530 535 540Ser Val Glu Ala Ala Glu Ala Ile Gln Asp Val Asp Ser Ala Ala Asp545 550 555 560Gly Asp Val Pro Glu Thr Asp Ser Leu Ser Asp Ile Asp Leu Glu Thr
565 570 575Leu Glu Lys Phe Glu Glu Asp Val Glu Asn Gly Thr Asp Ile Asp Gln
580 585 590Val Ser Gly Asp Tyr Tyr Gly Thr Asp Asp Gln Gly Gly Thr Ala Pro
595 600 605Ser Lys Glu
610<210>55<211>1446<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1423)<223>RXA02630<400>55gcgggttcga aaatgtcgat gattaaacca ctaaagagct cacaggaagt gttcagacta 60cttagagtga cgccccagcc acagggttca taatcaaatc atg aca aat caa ttc 115
Met Thr Asn Gln Phe
1 5ccc aca aac aac ggt gag aac ccg gac cgt gca tcg gaa act cca tca 163Pro Thr Asn Asn Gly Glu Asn Pro Asp Arg Ala Ser Glu Thr Pro Ser
10 15 20gaa acc aac tcc ttc gaa cat gtg cgt agt tca tat ccg cag tgg ggt 211Glu Thr Asn Ser Phe Glu His Val Arg Ser Ser Tyr Pro Gln Trp Gly
25 30 35aac act gct tcc aat caa aac ccc tat cct ggt gcg ggc ttc ggc tct 259Asn Thr Ala Ser Asn Gln Asn Pro Tyr Pro Gly Ala Gly Phe Gly Ser
40 45 50gaa caa aac act caa caa gga aat gag caa caa gct cca gcc tgg acc 307Glu Gln Asn Thr Gln Gln Gly Asn Glu Gln Gln Ala Pro Ala Trp Thr
55 60 65agt tgg gat aat cag cct cta agc aca gat gta aag cca gct aaa gaa 355Ser Trp Asp Asn Gln Pro Leu Ser Thr Asp Val Lys Pro Ala Lys Glu70 75 80 85aag cga aaa gtt ggc atc gga acg gca ctc gcg tta atg ctt gtt ggt 403Lys Arg Lys Val Gly Ile Gly Thr Ala Leu Ala Leu Met Leu Val Gly
90 95 100tct att gct acc ggt agc gtt gtg ggt gtt gca gca acc cag ctt ggt 451Ser Ile Ala Thr Gly Ser Val Val Gly Val Ala Ala Thr Gln Leu Gly
105 110 115tcg gac tct tca acc cca gtt aat gct ctt gag cag ccc agc gtg cag 499Ser Asp Ser Ser Thr Pro Val Asn Ala Leu Glu Gln Pro Ser Val Gln
120 125 130cgc acc act aat gct gaa cca ggt tca gcg gaa cag gtt gct gcc gca 547Arg Thr Thr Asn Ala Glu Pro Gly Ser Ala Glu Gln Val Ala Ala Ala
135 140 145gtt ttg cct tct gtc gtc tct att cag gcc att act agg acg tct gct 595Val Leu Pro Ser Val Val Ser Ile Gln Ala Ile Thr Arg Thr Ser Ala150 155 160 165tct gag ggc tct gga tcc att att tcc tct gat ggt tac gtc atg acc 643Ser Glu Gly Ser Gly Ser Ile Ile Ser Ser Asp Gly Tyr Val Met Thr
170 175 180aat aat cac gtc gtg gca ggc att gaa caa tct ggt gtg tta gaa gta 691Asn Asn His Val Val Ala Gly Ile Glu Gln Ser Gly Val Leu Glu Val
185 190 195agt ttc tcc gat gga act aca gcg caa gct gat ttt att gct ggt gat 739Ser Phe Ser Asp Gly Thr Thr Ala Gln Ala Asp Phe Ile Ala Gly Asp
200 205 210cct tcc aca gat att gct gtg atc aag att agg gat gtg tcc aac ctt 787Pro Ser Thr Asp Ile Ala Val Ile Lys Ile Arg Asp Val Ser Asn Leu
215 220 225cca gtt atg agc ttt gga gat tcg gac gca tta ggc gtt gga caa agt 835Pro Val Met Ser Phe Gly Asp Ser Asp Ala Leu Gly Val Gly Gln Ser230 235 240 245gtg atg gct gtt ggt tct cca ctg ggt ctg agc tcc act gtg acc acc 883Val Met Ala Val Gly Ser Pro Leu Gly Leu Ser Ser Thr Val Thr Thr
250 255 260ggt att gtg tcg gcc gtg aac cgt cct gtg cga gct tct ggt gat ggc 931Gly Ile Val Ser Ala Val Asn Arg Pro Val Arg Ala Ser Gly Asp Gly
265 270 275gga gag tcg tcc ctc atc gat gct atc cag acc gat gct gcg atc aac 979Gly Glu Ser Ser Leu Ile Asp Ala Ile Gln Thr Asp Ala Ala Ile Asn
280 285 290cct ggt aac tct ggt ggt ccg ctg gtt gat atg gat ggc aac ctc att 1027Pro Gly Asn Ser Gly Gly Pro Leu Val Asp Met Asp Gly Asn Leu Ile
295 300 305ggc atg aat tcg gta att gca tcg att tcg agc acc agc gat tcc gca 1075Gly Met Asn Ser Val Ile Ala Ser Ile Ser Ser Thr Ser Asp Ser Ala310 315 320 325ggt tcc att ggt ctt ggt ttt tct atc cca tcc aac ttt gcc aag cgc 1123Gly Ser Ile Gly Leu Gly Phe Ser Ile Pro Ser Asn Phe Ala Lys Arg
330 335 340gtg gcc gat caa ttg atc agc acc ggc cag gta act cag ccg atg atc 1171Val Ala Asp Gln Leu Ile Ser Thr Gly Gln Val Thr Gln Pro Met Ile
345 350 355ggt gtg cag gtt ggc act gac aac tca gtg aca ggc gct gtg att gcc 1219Gly Val Gln Val Gly Thr Asp Asn Ser Val Thr Gly Ala Val Ile Ala
360 365 370agt gtt caa gat ggt gga ccg gcc gca gat gct gga ctt cag cca ggc 1267Ser Val Gln Asp Gly Gly Pro Ala Ala Asp Ala Gly Leu Gln Pro Gly
375 380 385gat atc gtg acc aag ctc aat gat cga gtt att gat agc cca gac tcc 1315Asp Ile Val Thr Lys Leu Asn Asp Arg Val Ile Asp Ser Pro Asp Ser390 395 400 405ttg atc gct gct gtt cgt tcg cat gat ttt ggc gaa acc gtc act tta 1363Leu Ile Ala Ala Val Arg Ser His Asp Phe Gly Glu Thr Val Thr Leu
410 415 420aca att aca cag cca gat acc tcg cag agc cgg gag gta gag gtt act 1411Thr Ile Thr Gln Pro Asp Thr Ser Gln Ser Arg Glu Val Glu Val Thr
425 430 435ctg acg agt gag taggtttaaa agagttaatc tgc 1446Leu Thr Ser Glu
440<210>56<211>441<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>56Met Thr Asn Gln Phe Pro Thr Asn Asn Gly Glu Asn Pro Asp Arg Ala1 5 10 15Ser Glu Thr Pro Ser Glu Thr Asn Ser Phe Glu His Val Arg Ser Ser
20 25 30Tyr Pro Gln Trp Gly Asn Thr Ala Ser Asn Gln Asn Pro Tyr Pro Gly
35 40 45Ala Gly Phe Gly Ser Glu Gln Asn Thr Gln Gln Gly Asn Glu Gln Gln
50 55 60Ala Pro Ala Trp Thr Ser Trp Asp Asn Gln Pro Leu Ser Thr Asp Val65 70 75 80Lys Pro Ala Lys Glu Lys Arg Lys Val Gly Ile Gly Thr Ala Leu Ala
85 90 95Leu Met Leu Val Gly Ser Ile Ala Thr Gly Ser Val Val Gly Val Ala
100 105 110Ala Thr Gln Leu Gly Ser Asp Ser Ser Thr Pro Val Asn Ala Leu Glu
115 120 125Gln Pro Ser Val Gln Arg Thr Thr Asn Ala Glu Pro Gly Ser Ala Glu
130 135 140Gln Val Ala Ala Ala Val Leu Pro Ser Val Val Ser Ile Gln Ala Ile145 150 155 160Thr Arg Thr Ser Ala Ser Glu Gly Ser Gly Ser Ile Ile Ser Ser Asp
165 170 175Gly Tyr Val Met Thr Asn Asn His Val Val Ala Gly Ile Glu Gln Ser
180 185 190Gly Val Leu Glu Val Ser Phe Ser Asp Gly Thr Thr Ala Gln Ala Asp
195 200 205Phe Ile Ala Gly Asp Pro Ser Thr Asp Ile Ala Val Ile Lys Ile Arg
210 215 220Asp Val Ser Asn Leu Pro Val Met Ser Phe Gly Asp Ser Asp Ala Leu225 230 235 240Gly Val Gly Gln Ser Val Met Ala Val Gly Ser Pro Leu Gly Leu Ser
245 250 255Ser Thr Val Thr Thr Gly Ile Val Ser Ala Val Asn Arg Pro Val Arg
260 265 270Ala Ser Gly Asp Gly Gly Glu Ser Ser Leu Ile Asp Ala Ile Gln Thr
275 280 285Asp Ala Ala Ile Asn Pro Gly Asn Ser Gly Gly Pro Leu Val Asp Met
290 295 300Asp Gly Asn Leu Ile Gly Met Asn Ser Val Ile Ala Ser Ile Ser Ser305 310 315 320Thr Ser Asp Ser Ala Gly Ser Ile Gly Leu Gly Phe Ser Ile Pro Ser
325 330 335Asn Phe Ala Lys Arg Val Ala Asp Gln Leu Ile Ser Thr Gly Gln Val
340 345 350Thr Gln Pro Met Ile Gly Val Gln Val Gly Thr Asp Asn Ser Val Thr
355 360 365Gly Ala Val Ile Ala Ser Val Gln Asp Gly Gly Pro Ala Ala Asp Ala
370 375 380Gly Leu Gln Pro Gly Asp Ile Val Thr Lys Leu Asn Asp Arg Val Ile385 390 395 400Asp Ser Pro Asp Ser Leu Ile Ala Ala Val Arg Ser His Asp Phe Gly
405 410 415Glu Thr Val Thr Leu Thr Ile Thr Gln Pro Asp Thr Ser Gln Ser Arg
420 425 430Glu Val Glu Val Thr Leu Thr Ser Glu
435 440<210>57<211>518<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(495)<223>RXA02834<400>57gat tct aaa ggt aga agt gtt gac ttt aaa aat acc att atc atc atg 48Asp Ser Lys Gly Arg Ser Val Asp Phe Lys Asn Thr Ile Ile Ile Met1 5 10 15act agt aat att ggt tca caa gta tta ctt gaa aat gta aaa gat gct 96Thr Ser Asn Ile Gly Ser Gln Val Leu Leu Glu Asn Val Lys Asp Ala
20 25 30ggt gaa att agt gat gat aca gag aaa gca gtt atg gac agt cta cat 144Gly Glu Ile Ser Asp Asp Thr Glu Lys Ala Val Met Asp Ser Leu His
35 40 45gca tac ttc aaa cct gaa ata tta aat cgt atg gat gac atc gtg tta 192Ala Tyr Phe Lys Pro Glu Ile Leu Asn Arg Met Asp Asp Ile Val Leu
50 55 60ttt aaa cca tta tca gtt aat gat atg agt atg att gta gat aaa att 240Phe Lys Pro Leu Ser Val Asn Asp Met Ser Met Ile Val Asp Lys Ile65 70 75 80tta aca caa tta aat atg aga tta tta gat caa cat atc tca att gaa 288Leu Thr Gln Leu Asn Met Arg Leu Leu Asp Gln His Ile Ser Ile Glu
85 90 95gtg aca gaa gaa gcg aaa aaa tgg cta ggt gaa gaa gcg tat gaa cca 336Val Thr Glu Glu Ala Lys Lys Trp Leu Gly Glu Glu Ala Tyr Glu Pro
100 105 110caa ttt ggt gca aga cca tta aaa cgc ttt gtt caa cga caa ata gaa 384Gln Phe Gly Ala Arg Pro Leu Lys Arg Phe Val Gln Arg Gln Ile Glu
115 120 125act cca att gca cgt atg atg att aaa gaa agt cta cct gaa ggt aca 432Thr Pro Ile Ala Arg Met Met Ile Lys Glu Ser Leu Pro Glu Gly Thr
130 135 140ata att aaa gta gat tta aat gac aat aaa gaa ctt gat ttt aag gtt 480Ile Ile Lys Val Asp Leu Asn Asp Asn Lys Glu Leu Asp Phe Lys Val145 150 155 160gtt aaa cct acg tct taatctagca aaatattaat ttg 518Val Lys Pro Thr Ser
165<210>58<211>165<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>58Asp Ser Lys Gly Arg Ser Val Asp Phe Lys Asn Thr Ile Ile Ile Met1 5 10 15Thr Ser Asn Ile Gly Ser Gln Val Leu Leu Glu Asn Val Lys Asp Ala
20 25 30Gly Glu Ile Ser Asp Asp Thr Glu Lys Ala Val Met Asp Ser Leu His
35 40 45Ala Tyr Phe Lys Pro Glu Ile Leu Asn Arg Met Asp Asp Ile Val Leu
50 55 60Phe Lys Pro Leu Ser Val Asn Asp Met Ser Met Ile Val Asp Lys Ile65 70 75 80Leu Thr Gln Leu Asn Met Arg Leu Leu Asp Gln His Ile Ser Ile Glu
85 90 95Val Thr Glu Glu Ala Lys Lys Trp Leu Gly Glu Glu Ala Tyr Glu Pro
100 105 110Gln Phe Gly Ala Arg Pro Leu Lys Arg Phe Val Gln Arg Gln Ile Glu
115 120 125Thr Pro Ile Ala Arg Met Met Ile Lys Glu Ser Leu Pro Glu Gly Thr
130 135 140Ile Ile Lys Val Asp Leu Asn Asp Asn Lys Glu Leu Asp Phe Lys Val145 150 155 160Val Lys Pro Thr Ser
165<210>59<211>1314<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1291)<223>RXA00112<400>59gcgttaggct gacgggctcc ggtggcctaa aatcgaagcg atctaaaaat ttggcagtta 60atattatcga ttttcgaaag cgtagaacac caaaccattg ttg agc ccc agc ctg 115
Leu Ser Pro Ser Leu
1 5gtc gtc gat gcc gtc atc gtc ctc gtt atg gca ttc gcc ctg tgg ggt 163Val Val Asp Ala Val Ile Val Leu Val Met Ala Phe Ala Leu Trp Gly
10 15 20ggt tgg cgt caa ggc gcc ttc acc tcg ctg ctg tcc acc gtc ggc gtc 211Gly Trp Arg Gln Gly Ala Phe Thr Ser Leu Leu Ser Thr Val Gly Val
25 30 35gtt tct ggc ctg gta gtt ggc gca gca gca gct cca ttt gtc atg ggt 259Val Ser Gly Leu Val Val Gly Ala Ala Ala Ala Pro Phe Val Met Gly
40 45 50ctc acc gat tcc acc gcg ctt cgc ttc ctc ctg gcg atc ggc acc gtg 307Leu Thr Asp Ser Thr Ala Leu Arg Phe Leu Leu Ala Ile Gly Thr Val
55 60 65gtg ctg ctg gtt ggt ttg gga aat ctc atc ggc gcc cac ttg ggt gct 355Val Leu Leu Val Gly Leu Gly Asn Leu Ile Gly Ala His Leu Gly Ala70 75 80 85gcg att aga gac aac atc aaa ttc cga agt tcc agg acc tta gat tct 403Ala Ile Arg Asp Asn Ile Lys Phe Arg Ser Ser Arg Thr Leu Asp Ser
90 95 100ggg ctc ggc gcc att ttc caa gta ttg gcc acc ttg atc gtg gtg tgg 451Gly Leu Gly Ala Ile Phe Gln Val Leu Ala Thr Leu Ile Val Val Trp
105 110 115ctc gtc gca att ccc ctg gcc aca ggc ctc ccc gga act gtc gcc agc 499Leu Val Ala Ile Pro Leu Ala Thr Gly Leu Pro Gly Thr Val Ala Ser
120 125 130gga att aga gac tcc cgc atc ctg ggc ttt gta gac aaa tac acc ccg 547Gly Ile Arg Asp Ser Arg Ile Leu Gly Phe Val Asp Lys Tyr Thr Pro
135 140 145caa ggc cta gat acc ctg ccc tcc aaa atc gct gcg atg ctc agc gaa 595Gln Gly Leu Asp Thr Leu Pro Ser Lys Ile Ala Ala Met Leu Ser Glu150 155 160 165tcc ggc ctc cca cca ctg att tcc ccc ttc acc ggc gga tcc tcg gtg 643Ser Gly Leu Pro Pro Leu Ile Ser Pro Phe Thr Gly Gly Ser Ser Val
170 175 180gaa gtg gac gcc ccc gaa atc aac gtc acc aac gtt gac cta gtc gaa 691Glu Val Asp Ala Pro Glu Ile Asn Val Thr Asn Val Asp Leu Val Glu
185 190 195gca atg cgc ccg tcc gtc atc cac gtg atg ggt gac gcc caa gaa tgc 739Ala Met Arg Pro Ser Val Ile His Val Met Gly Asp Ala Gln Glu Cys
200 205 210agc cgc cga ctc atg ggt tct ggc ttt gtg gca tcc ccc gac tac gtt 787Ser Arg Arg Leu Met Gly Ser Gly Phe Val Ala Ser Pro Asp Tyr Val
215 220 225gtg acc aac gcc cac gtt gtt gca ggt acc tcc acc gtc agc ctg gat 835Val Thr Asn Ala His Val Val Ala Gly Thr Ser Thr Val Ser Leu Asp230 235 240 245acc atg atc gga acc cgc tcc gca gag gta gtg ttc tac gac ccg aac 883Thr Met Ile Gly Thr Arg Ser Ala Glu Val Val Phe Tyr Asp Pro Asn
250 255 260ctg gac atc gca gtc ctt tac agc cct gac ctc ggc ttg gat cca ctg 931Leu Asp Ile Ala Val Leu Tyr Ser Pro Asp Leu Gly Leu Asp Pro Leu
265 270 275ccg tgg gca tcc act ccg cta gac act ggc gat gaa gca atc gtc atg 979Pro Trp Ala Ser Thr Pro Leu Asp Thr Gly Asp Glu Ala Ile Val Met
280 285 290gga ttc cca cag tcc gga cct ttc aac gcc tcc cca gcc agg gtc cgc 1027Gly Phe Pro Gln Ser Gly Pro Phe Asn Ala Ser Pro Ala Arg Val Arg
295 300 305gaa cgc atc atg atc acc ggc agc aac att tac gcc aac ggc cag cac 1075Glu Arg Ile Met Ile Thr Gly Ser Asn Ile Tyr Ala Asn Gly Gln His310 315 320 325gaa cgc gaa gcc tat tca gtc cgc gga tcc atc caa tct gga aac tcc 1123Glu Arg Glu Ala Tyr Ser Val Arg Gly Ser Ile Gln Ser Gly Asn Ser
330 335 340ggc ggc cca atg acc aac gaa atg ggt gaa gtg gtt ggt gtt gtc ttc 1171Gly Gly Pro Met Thr Asn Glu Met Gly Glu Val Val Gly Val Val Phe
345 350 355ggc gca gcg atc gac ggc tcc gat acc ggt tac gtt ctc act gcc gaa 1219Gly Ala Ala Ile Asp Gly Ser Asp Thr Gly Tyr Val Leu Thr Ala Glu
360 365 370gag gta cag gag cgg atc ggc gac atc acc gcg ctg act cag cct gtc 1267Glu Val Gln Glu Arg Ile Gly Asp Ile Thr Ala Leu Thr Gln Pro Val
375 380 385gat acg atg cag tgc gcg gtt tct tagtcgtcgg gagctaggac cag 1314Asp Thr Met Gln Cys Ala Val Ser390 395<210>60<211>397212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>60Leu Ser Pro Ser Leu Val Val Asp Ala Val Ile Val Leu Val Met Ala1 5 10 15Phe Ala Leu Trp Gly Gly Trp Arg Gln Gly Ala Phe Thr Ser Leu Leu
20 25 30Ser Thr Val Gly Val Val Ser Gly Leu Val Val Gly Ala Ala Ala Ala
35 40 45Pro Phe Val Met Gly Leu Thr Asp Ser Thr Ala Leu Arg Phe Leu Leu
50 55 60Ala Ile Gly Thr Val Val Leu Leu Val Gly Leu Gly Asn Leu Ile Gly65 70 75 80Ala His Leu Gly Ala Ala Ile Arg Asp Asn Ile Lys Phe Arg Ser Ser
85 90 95Arg Thr Leu Asp Ser Gly Leu Gly Ala Ile Phe Gln Val Leu Ala Thr
100 105 110Leu Ile Val Val Trp Leu Val Ala Ile Pro Leu Ala Thr Gly Leu Pro
115 120 125Gly Thr Val Ala Ser Gly Ile Arg Asp Ser Arg Ile Leu Gly Phe Val
130 135 140Asp Lys Tyr Thr Pro Gln Gly Leu Asp Thr Leu Pro Ser Lys Ile Ala145 150 155 160Ala Met Leu Ser Glu Ser Gly Leu Pro Pro Leu Ile Ser Pro Phe Thr
165 170 175Gly Gly Ser Ser Val Glu Val Asp Ala Pro Glu Ile Asn Val Thr Asn
180 185 190Val Asp Leu Val Glu Ala Met Arg Pro Ser Val Ile His Val Met Gly
195 200 205Asp Ala Gln Glu Cys Ser Arg Arg Leu Met Gly Ser Gly Phe Val Ala
210 215 220Ser Pro Asp Tyr Val Val Thr Asn Ala His Val Val Ala Gly Thr Ser225 230 235 240Thr Val Ser Leu Asp Thr Met Ile Gly Thr Arg Ser Ala Glu Val Val
245 250 255Phe Tyr Asp Pro Asn Leu Asp Ile Ala Val Leu Tyr Ser Pro Asp Leu
260 265 270Gly Leu Asp Pro Leu Pro Trp Ala Ser Thr Pro Leu Asp Thr Gly Asp
275 280 285Glu Ala Ile Val Met Gly Phe Pro Gln Ser Gly Pro Phe Asn Ala Ser
290 295 300Pro Ala Arg Val Arg Glu Arg Ile Met Ile Thr Gly Ser Asn Ile Tyr305 310 315 320Ala Asn Gly Gln His Glu Arg Glu Ala Tyr Ser Val Arg Gly Ser Ile
325 330 335Gln Ser Gly Asn Ser Gly Gly Pro Met Thr Asn Glu Met Gly Glu Val
340 345 350Val Gly Val Val Phe Gly Ala Ala Ile Asp Gly Ser Asp Thr Gly Tyr
355 360 365Val Leu Thr Ala Glu Glu Val Gln Glu Arg Ile Gly Asp Ile Thr Ala
370 375 380Leu Thr Gln Pro Val Asp Thr Met Gln Cys Ala Val Ser385 390 395<210>61<211>729<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(706)<223>RXA00566<400>61cttgttgacc acgtgattac cttggctgaa ggcccaatca gcaactaggc gcacggaaaa 60ctttaaagga gagaataaga ttatgagcag cggattccaa atg cca acg tcc cgt 115
Met Pro Thr Ser Arg
1 5tac gtg ctg cct tcc ttc att gag cag tcc gca tac ggc acc aaa gag 163Tyr Val Leu Pro Ser Phe Ile Glu Gln Ser Ala Tyr Gly Thr Lys Glu
10 15 20acc aac cct tac gca aaa ctc ttt gaa gag cgc atc atc ttc ctg ggc 211Thr Asn Pro Tyr Ala Lys Leu Phe Glu Glu Arg Ile Ile Phe Leu Gly
25 30 35acc cag gtc gac gac acc tct gca aac gac atc atg gcg cag ctc ctt 259Thr Gln Val Asp Asp Thr Ser Ala Asn Asp Ile Met Ala Gln Leu Leu
40 45 50gtc ctc gaa ggc atg gac cca gac cgc gat atc acc ctg tac atc aac 307Val Leu Glu Gly Met Asp Pro Asp Arg Asp Ile Thr Leu Tyr Ile Asn
55 60 65tca cct ggt gga tcc ttc acc gcg ttg atg gca att tac gac acc atg 355Ser Pro Gly Gly Ser Phe Thr Ala Leu Met Ala Ile Tyr Asp Thr Met70 75 80 85cag tac gtc cgc cca gac gtt cag acc gtc tgc ctt ggt cag gca gca 403Gln Tyr Val Arg Pro Asp Val Gln Thr Val Cys Leu Gly Gln Ala Ala
90 95 100tcc gca gcc gca gtt ctt ctt gca gcc ggt gca cca ggt aag cgc gct 451Ser Ala Ala Ala Val Leu Leu Ala Ala Gly Ala Pro Gly Lys Arg Ala
105 110 115gtt ctt cct aac tcc cgc gtg ctg atc cac cag cca gca acc cag ggc 499Val Leu Pro Asn Ser Arg Val Leu Ile His Gln Pro Ala Thr Gln Gly
120 125 130acc cag ggt cag gtt tct gac ctc gag atc cag gca gct gaa atc gag 547Thr Gln Gly Gln Val Ser Asp Leu Glu Ile Gln Ala Ala Glu Ile Glu
135 140 145cgc atg cgt cgt ttg atg gaa acc acc ttg gct gag cac acc ggc aag 595Arg Met Arg Arg Leu Met Glu Thr Thr Leu Ala Glu His Thr Gly Lys150 155 160 165acc gcg gag cag atc cgc atc gat acc gac cgt gac aag atc ctc acc 643Thr Ala Glu Gln Ile Arg Ile Asp Thr Asp Arg Asp Lys Ile Leu Thr
170 175 180gct gag gaa gca ctc gag tat ggc atc gtt gac cag gtc ttc gat tac 691Ala Glu Glu Ala Leu Glu Tyr Gly Ile Val Asp Gln Val Phe Asp Tyr
185 190 195cgc aag ctc aag cgc tagagttttt taaagattcg ggt 729Arg Lys Leu Lys Arg
200<210>62<211>202<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>62Met Pro Thr Ser Arg Tyr Val Leu Pro Ser Phe Ile Glu Gln Ser Ala1 5 10 15Tyr Gly Thr Lys Glu Thr Asn Pro Tyr Ala Lys Leu Phe Glu Glu Arg
20 25 30Ile Ile Phe Leu Gly Thr Gln Val Asp Asp Thr Ser Ala Asn Asp Ile
35 40 45Met Ala Gln Leu Leu Val Leu Glu Gly Met Asp Pro Asp Arg Asp Ile
50 55 60Thr Leu Tyr Ile Asn Ser Pro Gly Gly Ser Phe Thr Ala Leu Met Ala65 70 75 80Ile Tyr Asp Thr Met Gln Tyr Val Arg Pro Asp Val Gln Thr Val Cys
85 90 95Leu Gly Gln Ala Ala Ser Ala Ala Ala Val Leu Leu Ala Ala Gly Ala
100 105 110Pro Gly Lys Arg Ala Val Leu Pro Asn Ser Arg Val Leu Ile His Gln
115 120 125Pro Ala Thr Gln Gly Thr Gln Gly Gln Val Ser Asp Leu Glu Ile Gln
130 135 140Ala Ala Glu Ile Glu Arg Met Arg Arg Leu Met Glu Thr Thr Leu Ala145 150 155 160Glu His Thr Gly Lys Thr Ala Glu Gln Ile Arg Ile Asp Thr Asp Arg
165 170 175Asp Lys Ile Leu Thr Ala Glu Glu Ala Leu Glu Tyr Gly Ile Val Asp
180 185 190Gln Val Phe Asp Tyr Arg Lys Leu Lys Arg
195 200<210>63<211>714<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(691)<223>RXA00567<400>63cgaacagagg cggtttcatg gaaataccgc cgggtagtct ggtgacattg aaccaaatga 60acgtacccaa gatttaagaa tgtaggagtt gactgttttc atg agc gat att cgt 115
Met Ser Asp Ile Arg
1 5atg gca gcc cag ggt ggg cct ggt ttc gga aat gac gtc ttt gat cgc 163Met Ala Ala Gln Gly Gly Pro Gly Phe Gly Asn Asp Val Phe Asp Arg
10 15 20ctc atg agt gag cgc atc att ttc ctg gga agc cag gta gac gat gag 211Leu Met Ser Glu Arg Ile Ile Phe Leu Gly Ser Gln Val Asp Asp Glu
25 30 35att gca aac aag cta tgc gct cag atc ctg ctg ctg tcc gct gag gat 259Ile Ala Asn Lys Leu Cys Ala Gln Ile Leu Leu Leu Ser Ala Glu Asp
40 45 50cca acc agg gac atc tcc ctg tac att aac tcc cca ggt ggc tcc gtc 307Pro Thr Arg Asp Ile Ser Leu Tyr Ile Asn Ser Pro Gly Gly Ser Val
55 60 65acc gca ggc atg gca att tat gac acc atg aaa tac tcc cca tgc gac 355Thr Ala Gly Met Ala Ile Tyr Asp Thr Met Lys Tyr Ser Pro Cys Asp70 75 80 85atc gca acc tac ggc atg ggc ctg gca gca tcc atg ggc cag ttc ctg 403Ile Ala Thr Tyr Gly Met Gly Leu Ala Ala Ser Met Gly Gln Phe Leu
90 95 100ctt tcc ggt ggc act aag ggc aag cgt ttc gca ttg cca cac gca cgt 451Leu Ser Gly Gly Thr Lys Gly Lys Arg Phe Ala Leu Pro His Ala Arg
105 110 115atc atg atg cac cag cct tcc gct ggt gtt ggt ggt acc gca gct gat 499Ile Met Met His Gln Pro Ser Ala Gly Val Gly Gly Thr Ala Ala Asp
120 125 130atc gct atc cag gct gag cag ttc gca gcc acc aag cgt gaa atg gcc 547Ile Ala Ile Gln Ala Glu Gln Phe Ala Ala Thr Lys Arg Glu Met Ala
135 140 145cag ctg atc gct gag cac acc ggc cag acc ttt gag cag atc tcc aag 595Gln Leu Ile Ala Glu His Thr Gly Gln Thr Phe Glu Gln Ile Ser Lys150 155 160 165gat tcc gat cgt gac cgc tgg ttc act gca cag gaa gct aag gat tac 643Asp Ser Asp Arg Asp Arg Trp Phe Thr Ala Gln Glu Ala Lys Asp Tyr
170 175 180gga ctt gtt gac cac gtg att acc ttg gct gaa ggc cca atc agc aac 691Gly Leu Val Asp His Val Ile Thr Leu Ala Glu Gly Pro Ile Ser Asn
185 190 195taggcgcacg gaaaacttta aag 714<210>64<211>197<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>64Met Ser Asp Ile Arg Met Ala Ala Gln Gly Gly Pro Gly Phe Gly Asn1 5 10 15Asp Val Phe Asp Arg Leu Met Ser Glu Arg Ile Ile Phe Leu Gly Ser
20 25 30Gln Val Asp Asp Glu Ile Ala Asn Lys Leu Cys Ala Gln Ile Leu Leu
35 40 45Leu Ser Ala Glu Asp Pro Thr Arg Asp Ile Ser Leu Tyr Ile Asn Ser
50 55 60Pro Gly Gly Ser Val Thr Ala Gly Met Ala Ile Tyr Asp Thr Met Lys65 70 75 80Tyr Ser Pro Cys Asp Ile Ala Thr Tyr Gly Met Gly Leu Ala Ala Ser
85 90 95Met Gly Gln Phe Leu Leu Ser Gly Gly Thr Lys Gly Lys Arg Phe Ala
100 105 110Leu Pro His Ala Arg Ile Met Met His Gln Pro Ser Ala Gly Val Gly
115 120 125Gly Thr Ala Ala Asp Ile Ala Ile Gln Ala Glu Gln Phe Ala Ala Thr
130 135 140Lys Arg Glu Met Ala Gln Leu Ile Ala Glu His Thr Gly Gln Thr Phe145 150 155 160Glu Gln Ile Ser Lys Asp Ser Asp Arg Asp Arg Trp Phe Thr Ala Gln
165 170 175Glu Ala Lys Asp Tyr Gly Leu Val Asp His Val Ile Thr Leu Ala Glu
180 185 190Gly Pro Ile Ser Asn
195<210>65<211>1875<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1852)<223>RXN03094<400>65atagatatta gagagttaaa taatggcgct tgacctgcag gaaattgaga tcaacactga 60ttgtgtaggt tggcgcccaa caaagaaagg gcgttgaaag atg agt tca ttc aat 115
Met Ser Ser Phe Asn
1 5cca act acc aaa acc aat gaa gcc atg cag gct gct ctt cag cag gca 163Pro Thr Thr Lys Thr Asn Glu Ala Met Gln Ala Ala Leu Gln Gln Ala
10 15 20tcc tcg gct ggc aac cct gat att cgt cca gct cac ctg ttg gct gcc 211Ser Ser Ala Gly Asn Pro Asp Ile Arg Pro Ala His Leu Leu Ala Ala
25 30 35atc ttg gag caa act gat ggc gta gca gcg cca gtc ctc atg gct act 259Ile Leu Glu Gln Thr Asp Gly Val Ala Ala Pro Val Leu Met Ala Thr
40 45 50ggt gtg gat cct aag gag atc ctc gca gag gcc aag aag ttg gtt gct 307Gly Val Asp Pro Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Val Ala
55 60 65tct tac ccc aag gct tct ggc gcc aat atg gct aat cca aac ttc aac 355Ser Tyr Pro Lys Ala Ser Gly Ala Asn Met Ala Asn Pro Asn Phe Asn70 75 80 85cgg gat gcc ctc aat gcg ttc act gca gct cag gag ctt gcc ggt gag 403Arg Asp Ala Leu Asn Ala Phe Thr Ala Ala Gln Glu Leu Ala Gly Glu
90 95 100ttg ggc gat gag tac gtc tca acc gaa gta ctt ctt gcc ggt atc gct 451Leu Gly Asp Glu Tyr Val Ser Thr Glu Val Leu Leu Ala Gly Ile Ala
105 110 115cgc gga aag tct gat gct gcg gat ctg ttg acc aac aag ggt gca acc 499Arg Gly Lys Ser Asp Ala Ala Asp Leu Leu Thr Asn Lys Gly Ala Thr
120 125 130tat gac gcc atc aaa gag gct ttc cct tcg gtt cgt gga tct cag cgt 547Tyr Asp Ala Ile Lys Glu Ala Phe Pro Ser Val Arg Gly Ser Gln Arg
135 140 145gtc acc act cag gat cca gag gga cag ttc cag gct ttg gaa aag tac 595Val Thr Thr Gln Asp Pro Glu Gly Gln Phe Gln Ala Leu Glu Lys Tyr150 155 160 165tcc act gac ctg acc aag ctt gct cgt gaa ggc aag att gat cct gtt 643Ser Thr Asp Leu Thr Lys Leu Ala Arg Glu Gly Lys Ile Asp Pro Val
170 175 180att ggc cgt gac cag gaa att cgt cgc gtc gtt cag gtg ctt agc cgt 691Ile Gly Arg Asp Gln Glu Ile Arg Arg Val Val Gln Val Leu Ser Arg
185 190 195cgt acc aag aac aac cct gtt ctg atc ggt gag cca ggt gtc ggt aaa 739Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys
200 205 210acc gcc atc gtg gaa ggc ctt gca cgc cgc atc gtt gct ggt gac gtt 787Thr Ala Ile Val Glu Gly Leu Ala Arg Arg Ile Val Ala Gly Asp Val
215 220 225cca gaa tcc ctc aag ggc aaa act ctg atc agt ctt gat ctt ggt tcc 835Pro Glu Ser Leu Lys Gly Lys Thr Leu Ile Ser Leu Asp Leu Gly Ser230 235 240 245atg gtt gcc ggc gct aag tat cgc ggt gaa ttc gag gag cga ctg aag 883Met Val Ala Gly Ala Lys Tyr Arg Gly Glu Phe Glu Glu Arg Leu Lys
250 255 260gct gtt ctg gat gag atc aag gga gct aac ggc gaa gtc gtt acc ttc 931Ala Val Leu Asp Glu Ile Lys Gly Ala Asn Gly Glu Val Val Thr Phe
265 270 275atc gat gag ctg cac acc atc gtc ggc gct ggt gct tcg ggt gaa tcc 979Ile Asp Glu Leu His Thr Ile Val Gly Ala Gly Ala Ser Gly Glu Ser
280 285 290gcc atg gat gcc gga aac atg att aag cca ctg ctt gcc cgc ggt gag 1027Ala Met Asp Ala Gly Asn Met Ile Lys Pro Leu Leu Ala Arg Gly Glu
295 300 305ctg cgc ttg gtt ggt gcc acc acg ctg aat gag tac cgc aag tac atc 1075Leu Arg Leu Val Gly Ala Thr Thr Leu Asn Glu Tyr Arg Lys Tyr Ile310 315 320 325gaa aag gac gct gcc ctg gag cgt agg ttc cag cag gtt tat gtc ggt 1123Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Gln Val Tyr Val Gly
330 335 340gag cca acg gta gaa gat gcc atc ggt att ctt cgt gga ttg aag gaa 1171Glu Pro Thr Val Glu Asp Ala Ile Gly Ile Leu Arg Gly Leu Lys Glu
345 350 355cgc tac gag gtc cat cac ggt gtc cgc atc cag gac tcc gca ctg gtc 1219Arg Tyr Glu Val His His Gly Val Arg Ile Gln Asp Ser Ala Leu Val
360 365 370gcc gca gct gaa ctc tca aac cgc tat atc acc agc cgt ttc ctt cct 1267Ala Ala Ala Glu Leu Ser Asn Arg Tyr Ile Thr Ser Arg Phe Leu Pro
375 380 385gat aag gct att gac tta gtt gat gag gca gca tca cgc ctg cgc atg 1315Asp Lys Ala Ile Asp Leu Val Asp Glu Ala Ala Ser Arg Leu Arg Met390 395 400 405gag att gat tct tca cct cag gaa atc gat gag ctg gag cgt atc gtc 1363Glu Ile Asp Ser Ser Pro Gln Glu Ile Asp Glu Leu Glu Arg Ile Val
410 415 420cgc cgc ctc gag atc gaa gag atg gcg ctg tcc aag gaa tcc gat gca 1411Arg Arg Leu Glu Ile Glu Glu Met Ala Leu Ser Lys Glu Ser Asp Ala
425 430 435gct tcc aag gaa cgt cta gaa aag ctg cgc tcg gaa ctt gct gat gaa 1459Ala Ser Lys Glu Arg Leu Glu Lys Leu Arg Ser Glu Leu Ala Asp Glu
440 445 450cgc gaa aag ctc tct gag ttg aag gct cgt tgg cag aat gag aaa act 1507Arg Glu Lys Leu Ser Glu Leu Lys Ala Arg Trp Gln Asn Glu Lys Thr
455 460 465gct att gac gat gtc cgg gag atg aaa gaa gag ctg gaa gcg ctg cgt 1555Ala Ile Asp Asp Val Arg Glu Met Lys Glu Glu Leu Glu Ala Leu Arg470 475 480 485tct gag tcg gat att gca aaa cgt gac ggc aat tat tgt cgt gtc gca 1603Ser Glu Ser Asp Ile Ala Lys Arg Asp Gly Asn Tyr Cys Arg Val Ala
490 495 500aag ctt cgc tac ggc cga atc cct gag ctg gaa aag cag atc gag gat 1651Lys Leu Arg Tyr Gly Arg Ile Pro Glu Leu Glu Lys Gln Ile Glu Asp
505 510 515gca gaa tcc aag gtc gag gtc aat gaa aat gcc atg ctc act gag gag 1699Ala Glu Ser Lys Val Glu Val Asn Glu Asn Ala Met Leu Thr Glu Glu
520 525 530gtc acg cca gac acg atc gcc gat gtg gtt tcc gca tgg acg ggc att 1747Val Thr Pro Asp Thr Ile Ala Asp Val Val Ser Ala Trp Thr Gly Ile
535 540 545cct gca ggc aag atg atg cag ggt gag acc gag aag ctg ctc aac atg 1795Pro Ala Gly Lys Met Met Gln Gly Glu Thr Glu Lys Leu Leu Asn Met550 555 560 565gag cgc gtc ttg ggc aac cgt gtg gtc ggt cag cta gaa agc ggt aac 1843Glu Arg Val Leu Gly Asn Arg Val Val Gly Gln Leu Glu Ser Gly Asn
570 575 580tgc agt gtc tgacgcggtg cgtcgttcac gcg 1875Cys Ser Val<210>66<211>584<212>PRT<213>谷氨酸棒杆菌(corynebacterium glutamicum)<400> 66Met Ser Ser Phe Asn Pro Thr Thr Lys Thr Asn Glu Ala Met Gln Ala1 5 10 15Ala Leu Gln Gln Ala Ser Ser Ala Gly Asn Pro Asp Ile Arg Pro Ala
20 25 30His Leu Leu Ala Ala Ile Leu Glu Gln Thr Asp Gly Val Ala Ala Pro
35 40 45Val Leu Met Ala Thr Gly Val Asp Pro Lys Glu Ile Leu Ala Glu Ala
50 55 60Lys Lys Leu Val Ala Ser Tyr Pro Lys Ala Ser Gly Ala Asn Met Ala65 70 75 80Asn Pro Asn Phe Asn Arg Asp Ala Leu Asn Ala Phe Thr Ala Ala Gln
85 90 95Glu Leu Ala Gly Glu Leu Gly Asp Glu Tyr Val Ser Thr Glu Val Leu
100 105 110Leu Ala Gly Ile Ala Arg Gly Lys Ser Asp Ala Ala Asp Leu Leu Thr
115 120 125Asn Lys Gly Ala Thr Tyr Asp Ala Ile Lys Glu Ala Phe Pro Ser Val
130 135 140Arg Gly Ser Gln Arg Val Thr Thr Gln Asp Pro Glu Gly Gln Phe Gln145 150 155 160Ala Leu Glu Lys Tyr Ser Thr Asp Leu Thr Lys Leu Ala Arg Glu Gly
165 170 175Lys Ile Asp Pro Val Ile Gly Arg Asp Gln Glu Ile Arg Arg Val Val
180 185 190Gln Val Leu Ser Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu
195 200 205Pro Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu Ala Arg Arg Ile
210 215 220Val Ala Gly Asp Val Pro Glu Ser Leu Lys Gly Lys Thr Leu Ile Ser225 230 235 240Leu Asp Leu Gly Ser Met Val Ala Gly Ala Lys Tyr Arg Gly Glu Phe
245 250 255Glu Glu Arg Leu Lys Ala Val Leu Asp Glu Ile Lys Gly Ala Asn Gly
260 265 270Glu Val Val Thr Phe Ile Asp Glu Leu His Thr Ile Val Gly Ala Gly
275 280 285Ala Ser Gly Glu Ser Ala Met Asp Ala Gly Asn Met Ile Lys Pro Leu
290 295 300Leu Ala Arg Gly Glu Leu Arg Leu Val Gly Ala Thr Thr Leu Asn Glu305 310 315 320Tyr Arg Lys Tyr Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln
325 330 335Gln Val Tyr Val Gly Glu Pro Thr Val Glu Asp Ala Ile Gly Ile Leu
340 345 350Arg Gly Leu Lys Glu Arg Tyr Glu Val His His Gly Val Arg Ile Gln
355 360 365Asp Ser Ala Leu Val Ala Ala Ala Glu Leu Ser Asn Arg Tyr Ile Thr
370 375 380Ser Arg Phe Leu Pro Asp Lys Ala Ile Asp Leu Val Asp Glu Ala Ala385 390 395 400Ser Arg Leu Arg Met Glu Ile Asp Ser Ser Pro Gln Glu Ile Asp Glu
405 410 415Leu Glu Arg Ile Val Arg Arg Leu Glu Ile Glu Glu Met Ala Leu Ser
420 425 430Lys Glu Ser Asp Ala Ala Ser Lys Glu Arg Leu Glu Lys Leu Arg Ser
435 440 445Glu Leu Ala Asp Glu Arg Glu Lys Leu Ser Glu Leu Lys Ala Arg Trp
450 455 460Gln Asn Glu Lys Thr Ala Ile Asp Asp Val Arg Glu Met Lys Glu Glu465 470 475 480Leu Glu Ala Leu Arg Ser Glu Ser Asp Ile Ala Lys Arg Asp Gly Asn
485 490 495Tyr Cys Arg Val Ala Lys Leu Arg Tyr Gly Arg Ile Pro Glu Leu Glu
500 505 510Lys Gln Ile Glu Asp Ala Glu Ser Lys Val Glu Val Asn Glu Asn Ala
515 520 525Met Leu Thr Glu Glu Val Thr Pro Asp Thr Ile Ala Asp Val Val Ser
530 535 540Ala Trp Thr Gly Ile Pro Ala Gly Lys Met Met Gln Gly Glu Thr Glu545 550 555 560Lys Leu Leu Asn Met Glu Arg Val Leu Gly Asn Arg Val Val Gly Gln
565 570 575Leu Glu Ser Gly Asn Cys Ser Val
580<210>67<211>1816<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1816)<223>FRXA01668<400>67atagatatta gagagttaaa taatggcgct tgacctgcag gaaattgaga tcaacactga 60ttgtgtaggt tggcgcccaa caaagaaagg gcgttgaaag atg agt tca ttc aat 115
Met Ser Ser Phe Asn
1 5cca act acc aaa acc aat gaa gcc atg cag gct gct ctt cag cag gca 163Pro Thr Thr Lys Thr Asn Glu Ala Met Gln Ala Ala Leu Gln Gln Ala
10 15 20tcc tcg gct ggc aac cct gat att cgt cca gct cac ctg ttg gct gcc 211Ser Ser Ala Gly Asn Pro Asp Ile Arg Pro Ala His Leu Leu Ala Ala
25 30 35atc ttg gag caa act gat ggc gta gca gcg cca gtc ctc atg gct act 259Ile Leu Glu Gln Thr Asp Gly Val Ala Ala Pro Val Leu Met Ala Thr
40 45 50ggt gtg gat cct aag gag atc ctc gca gag gcc aag aag ttg gtt gct 307Gly Val Asp Pro Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Val Ala
55 60 65tct tac ccc aag gct tct ggc gcc aat atg gct aat cca aac ttc aac 355Ser Tyr Pro Lys Ala Ser Gly Ala Asn Met Ala Asn Pro Asn Phe Asn70 75 80 85cgg gat gcc ctc aat gcg ttc act gca gct cag gag ctt gcc ggt gag 403Arg Asp Ala Leu Asn Ala Phe Thr Ala Ala Gln Glu Leu Ala Gly Glu
90 95 100ttg ggc gat gag tac gtc tca acc gaa gta ctt ctt gcc ggt atc gct 451Leu Gly Asp Glu Tyr Val Ser Thr Glu Val Leu Leu Ala Gly Ile Ala
105 110 115cgc gga aag tct gat gct gcg gat ctg ttg acc aac aag ggt gca acc 499Arg Gly Lys Ser Asp Ala Ala Asp Leu Leu Thr Asn Lys Gly Ala Thr
120 125 130tat gac gcc atc aaa gag gct ttc cct tcg gtt cgt gga tct cag cgt 547Tyr Asp Ala Ile Lys Glu Ala Phe Pro Ser Val Arg Gly Ser Gln Arg
135 140 145gtc acc act cag gat cca gag gga cag ttc cag gct ttg gaa aag tac 595Val Thr Thr Gln Asp Pro Glu Gly Gln Phe Gln Ala Leu Glu Lys Tyr150 155 160 165tcc act gac ctg acc aag ctt gct cgt gaa ggc aag att gat cct gtt 643Ser Thr Asp Leu Thr Lys Leu Ala Arg Glu Gly Lys Ile Asp Pro Val
170 175 180att ggc cgt gac cag gaa att cgt cgc gtc gtt cag gtg ctt agc cgt 691Ile Gly Arg Asp Gln Glu Ile Arg Arg Val Val Gln Val Leu Ser Arg
185 190 195cgt acc aag aac aac cct gtt ctg atc ggt gag cca ggt gtc ggt aaa 739Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys
200 205 210acc gcc atc gtg gaa ggc ctt gca cgc cgc atc gtt gct ggt gac gtt 787Thr Ala Ile Val Glu Gly Leu Ala Arg Arg Ile Val Ala Gly Asp Val
215 220 225cca gaa tcc ctc aag ggc aaa act ctg atc agt ctt gat ctt ggt tcc 835Pro Glu Ser Leu Lys Gly Lys Thr Leu Ile Ser Leu Asp Leu Gly Ser230 235 240 245atg gtt gcc ggc gct aag tat cgc ggt gaa ttc gag gag cga ctg aag 883Met Val Ala Gly Ala Lys Tyr Arg Gly Glu Phe Glu Glu Arg Leu Lys
250 255 260gct gtt ctg gat gag atc aag gga gct aac ggc gaa gtc gtt acc ttc 931Ala Val Leu Asp Glu Ile Lys Gly Ala Asn Gly Glu Val Val Thr Phe
265 270 275atc gat gag ctg cac acc atc gtc ggc gct ggt gct tcg ggt gaa tcc 979Ile Asp Glu Leu His Thr Ile Val Gly Ala Gly Ala Ser Gly Glu Ser
280 285 290gcc atg gat gcc gga aac atg att aag cca ctg ctt gcc cgc ggt gag 1027Ala Met Asp Ala Gly Asn Met Ile Lys Pro Leu Leu Ala Arg Gly Glu
295 300 305ctg cgc ttg gtt ggt gcc acc acg ctg aat gag tac cgc aag tac atc 1075Leu Arg Leu Val Gly Ala Thr Thr Leu Asn Glu Tyr Arg Lys Tyr Ile310 315 320 325gaa aag gac gct gcc ctg gag cgt agg ttc cag cag gtt tat gtc ggt 1123Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Gln Val Tyr Val Gly
330 335 340gag cca acg gta gaa gat gcc atc ggt att ctt cgt gga ttg aag gaa 1171Glu Pro Thr Val Glu Asp Ala Ile Gly Ile Leu Arg Gly Leu Lys Glu
345 350 355cgc tac gag gtc cat cac ggt gtc cgc atc cag gac tcc gca ctg gtc 1219Arg Tyr Glu Val His His Gly Val Arg Ile Gln Asp Ser Ala Leu Val
360 365 370gcc gca gct gaa ctc tca aac cgc tat atc acc agc cgt ttc ctt cct 1267Ala Ala Ala Glu Leu Ser Asn Arg Tyr Ile Thr Ser Arg Phe Leu Pro
375 380 385gat aag gct att gac tta gtt gat gag gca gca tca cgc ctg cgc atg 1315Asp Lys Ala Ile Asp Leu Val Asp Glu Ala Ala Ser Arg Leu Arg Met390 395 400 405gag att gat tct tca cct cag gaa atc gat gag ctg gag cgt atc gtc 1363Glu Ile Asp Ser Ser Pro Gln Glu Ile Asp Glu Leu Glu Arg Ile Val
410 415 420cgc cgc ctc gag atc gaa gag atg gcg ctg tcc aag gaa tcc gat gca 1411Arg Arg Leu Glu Ile Glu Glu Met Ala Leu Ser Lys Glu Ser Asp Ala
425 430 435gct tcc aag gaa cgt cta gaa aag ctg cgc tcg gaa ctt gct gat gaa 1459Ala Ser Lys Glu Arg Leu Glu Lys Leu Arg Ser Glu Leu Ala Asp Glu
440 445 450cgc gaa aag ctc tct gag ttg aag gct cgt tgg cag aat gag aaa act 1507Arg Glu Lys Leu Ser Glu Leu Lys Ala Arg Trp Gln Asn Glu Lys Thr
455 460 465gct att gac gat gtc cgg gag atg aaa gaa gag ctg gaa gcg ctg cgt 1555Ala Ile Asp Asp Val Arg Glu Met Lys Glu Glu Leu Glu Ala Leu Arg470 475 480 485tct gag tcg gat att gca aaa cgt gac ggc aat tat tgt cgt gtc gca 1603Ser Glu Ser Asp Ile Ala Lys Arg Asp Gly Asn Tyr Cys Arg Val Ala
490 495 500aag ctt cgc tac ggc cga atc cct gag ctg gaa aag cag atc gag gat 1651Lys Leu Arg Tyr Gly Arg Ile Pro Glu Leu Glu Lys Gln Ile Glu Asp
505 510 515gca gaa tcc aag gte gag gtc aat gaa aat gcc atg ctc act gag gag 1699Ala Glu Ser Lys Val Glu Val Asn Glu Asn Ala Met Leu Thr Glu Glu
520 525 530gtc acg cca gac acg atc gcc gat gtg gtt tcc gca tgg acg ggc att 1747Val Thr Pro Asp Thr Ile Ala Asp Val Val Ser Ala Trp Thr Gly Ile
535 540 545cct gca ggc aag atg atg cag ggt gag acc gag aag ctg ctc aac atg 1795Pro Ala Gly Lys Met Met Gln Gly Glu Thr Glu Lys Leu Leu Asn Met550 555 560 565gag cgc gtc ttg ggc aac ccg 1816Glu Arg Val Leu Gly Asn Pro
570<210>68<211>572<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>68Met Ser Ser Phe Asn Pro Thr Thr Lys Thr Asn Glu Ala Met Gln Ala1 5 10 15Ala Leu Gln Gln Ala Ser Ser Ala Gly Asn Pro Asp Ile Arg Pro Ala
20 25 30His Leu Leu Ala Ala Ile Leu Glu Gln Thr Asp Gly Val Ala Ala Pro
35 40 45Val Leu Met Ala Thr Gly Val Asp Pro Lys Glu Ile Leu Ala Glu Ala
50 55 60Lys Lys Leu Val Ala Ser Tyr Pro Lys Ala Ser Gly Ala Asn Met Ala65 70 75 80Asn Pro Asn Phe Asn Arg Asp Ala Leu Asn Ala Phe Thr Ala Ala Gln
85 90 95Glu Leu Ala Gly Glu Leu Gly Asp Glu Tyr Val Ser Thr Glu Val Leu
100 105 110Leu Ala Gly Ile Ala Arg Gly Lys Ser Asp Ala Ala Asp Leu Leu Thr
115 120 125Asn Lys Gly Ala Thr Tyr Asp Ala Ile Lys Glu Ala Phe Pro Ser Val
130 135 140Arg Gly Ser Gln Arg Val Thr Thr Gln Asp Pro Glu Gly Gln Phe Gln145 150 155 160Ala Leu Glu Lys Tyr Ser Thr Asp Leu Thr Lys Leu Ala Arg Glu Gly
165 170 175Lys Ile Asp Pro Val Ile Gly Arg Asp Gln Glu Ile Arg Arg Val Val
180 185 190Gln Val Leu Ser Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu
195 200 205Pro Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu Ala Arg Arg Ile
210 215 220Val Ala Gly Asp Val Pro Glu Ser Leu Lys Gly Lys Thr Leu Ile Ser225 230 235 240Leu Asp Leu Gly Ser Met Val Ala Gly Ala Lys Tyr Arg Gly Glu Phe
245 250 255Glu Glu Arg Leu Lys Ala Val Leu Asp Glu Ile Lys Gly Ala Asn Gly
260 265 270Glu Val Val Thr Phe Ile Asp Glu Leu His Thr Ile Val Gly Ala Gly
275 280 285Ala Ser Gly Glu Ser Ala Met Asp Ala Gly Asn Met Ile Lys Pro Leu
290 295 300Leu Ala Arg Gly Glu Leu Arg Leu Val Gly Ala Thr Thr Leu Asn Glu305 310 315 320Tyr Arg Lys Tyr Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln
325 330 335Gln Val Tyr Val Gly Glu Pro Thr Val Glu Asp Ala Ile Gly Ile Leu
340 345 350Arg Gly Leu Lys Glu Arg Tyr Glu Val His His Gly Val Arg Ile Gln
355 360 365Asp Ser Ala Leu Val Ala Ala Ala Glu Leu Ser Asn Arg Tyr Ile Thr
370 375 380Ser Arg Phe Leu Pro Asp Lys Ala Ile Asp Leu Val Asp Glu Ala Ala385 390 395 400Ser Arg Leu Arg Met Glu Ile Asp Ser Ser Pro Gln Glu Ile Asp Glu
405 410 415Leu Glu Arg Ile Val Arg Arg Leu Glu Ile Glu Glu Met Ala Leu Ser
420 425 430Lys Glu Ser Asp Ala Ala Ser Lys Glu Arg Leu Glu Lys Leu Arg Ser
435 440 445Glu Leu Ala Asp Glu Arg Glu Lys Leu Ser Glu Leu Lys Ala Arg Trp
450 455 460Gln Asn Glu Lys Thr Ala Ile Asp Asp Val Arg Glu Met Lys Glu Glu465 470 475 480Leu Glu Ala Leu Arg Ser Glu Ser Asp Ile Ala Lys Arg Asp Gly Asn
485 490 495Tyr Cys Arg Val Ala Lys Leu Arg Tyr Gly Arg Ile Pro Glu Leu Glu
500 505 510Lys Gln Ile Glu Asp Ala Glu Ser Lys Val Glu Val Asn Glu Asn Ala
515 520 525Met Leu Thr Glu Glu Val Thr Pro Asp Thr Ile Ala Asp Val Val Ser
530 535 540Ala Trp Thr Gly Ile Pro Ala Gly Lys Met Met Gln Gly Glu Thr Glu545 550 555 560Lys Leu Leu Asn Met Glu Arg Val Leu Gly Asn Pro
565 570<210>69<211>1401<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1378)<223>RXN01120<400>69acaggtaaag cgctaagatg gaacaaccca ttgccaatat tgttggttag agttgtacgc 60agtaaatctt ttcaatcgtg gaagcgggtc tcacagtcta atg gca cgt atg cag 115
Met Ala Arg Met Gln
1 5gaa agc gcc gat ctg ctc aaa tgt tcc ttc tgc gga aag agc caa aag 163Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys Gly Lys Ser Gln Lys
10 15 20cag gta aaa aaa ctc atc gcg ggt ggc gcc gta tat atc tgt gat gag 211Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val Tyr Ile Cys Asp Glu
25 30 35tgc att gag ctg tgc aac gag att att gaa gaa gaa ctc ggt caa gct 259Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu Glu Leu Gly Gln Ala
40 45 50caa cac gac gag cag gag cgc aac gag ctc ccc aag ccg tcg gag att 307Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro Lys Pro Ser Glu Ile
55 60 65tca gcc ttc ctt gat act tat gtc atc ggg cag gac cca gca aaa cgt 355Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln Asp Pro Ala Lys Arg70 75 80 85atc ctg tcg gtt gcg gtg tac aac cat tac aag cgt ctc cgc gca tcg 403Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys Arg Leu Arg Ala Ser
90 95 100gaa acc atc ggt cgt cgc agg aat gac gag cct gaa acc gaa ctg gtt 451Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro Glu Thr Glu Leu Val
105 110 115aag tcc aat att ttg atg ctc ggc ccc act ggc tcc ggc aag act ttc 499Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly Ser Gly Lys Thr Phe
120 125 130ctt gcc cag act ttg gca aag ctg ctg gat gtt cct ttt gct atc gcg 547Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val Pro Phe Ala Ile Ala
135 140 145gat gcc acc tca ctg acc gag gct ggt tat gtg ggc gag gat gtg gaa 595Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu150 155 160 165aac atc ttg ctc aag ctg ctt cag gct gct gat ttt gat gtg gaa cgt 643Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp Phe Asp Val Glu Arg
170 175 180gca cag cgc ggc atc att tac atc gat gaa gtg gac aag att tcc cgc 691Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val Asp Lys Ile Ser Arg
185 190 195aag tct gaa aac cca tcg atc act cgc gat gtt tcc ggt gaa ggc gtg 739Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val
200 205 210cag cag gca ctg ctg aaa att ttg gaa ggc act gtc gcc gca atc cca 787Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr Val Ala Ala Ile Pro
215 220 225ccg cag gga gga cgc aag cac ccc aac cag gat ttc atc cag ctg gat 835Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp Phe Ile Gln Leu Asp230 235 240 245acc acc aac att ttg ttc atc gtt gct ggt gcg ttc tct ggt ctg gag 883Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala Phe Ser Gly Leu Glu
250 255 260aag gtc atc gcg gac cgc aat ggc aag aaa ggc ttg ggc ttc ggt gtg 931Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly Leu Gly Phe Gly Val
265 270 275gag gtc tct tcc aag aag gaa gaa gcc aac att gtg gat atc ttc aag 979Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile Val Asp Ile Phe Lys
280 285 290gat gtc ctc cct gag gac ctg gtg aag ttt ggt ctc atc cca gaa ttc 1027Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly Leu Ile Pro Glu Phe
295 300 305att ggg cgt ctg cca gtc gtt gcc acc gta tcc aac ctg gat cag aaa 1075Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser Asn Leu Asp Gln Lys310 315 320 325tct ctg gtc aag gtt ctc acg gag cct cgt aac tca ttg gtg aag cag 1123Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn Ser Leu Val Lys Gln
330 335 340tat cga cgt ctg ttt gaa atg gat gac gct gtg ttg acc ttt act gat 1171Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val Leu Thr Phe Thr Asp
345 350 355gat gct ttg gag gag atc gct aat cag gca ctc gag cgc aaa act ggc 1219Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu Glu Arg Lys Thr Gly
360 365 370gcc cgt ggc ctg cgc gcg atc atg gaa gag atc ctg gtt ccg atc atg 1267Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile Leu Val Pro Ile Met
375 380 385tat gac ctc cca gac cgt aaa gac gtt ggc gaa gtc atc atc aac ggt 1315Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu Val Ile Ile Asn Gly390 395 400 405gcc gtt gcc cgt ggc gaa gcc gaa cca gag atg ttg gaa gct gtc gca 1363Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met Leu Glu Ala Val Ala
410 415 420gaa gaa aag acc gcg tagttggcag gagttatcac cgg 1401Glu Glu Lys Thr Ala
425<210>70<211>426<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>70Met Ala Arg Met Gln Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys1 5 10 15Gly Lys Ser Gln Lys Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val
20 25 30Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu
35 40 45Glu Leu Gly Gln Ala Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro
50 55 60Lys Pro Ser Glu Ile Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln65 70 75 80Asp Pro Ala Lys Arg Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys
85 90 95Arg Leu Arg Ala Ser Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro
100 105 110Glu Thr Glu Leu Val Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly
115 120 125Ser Gly Lys Thr Phe Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val
130 135 140Pro Phe Ala Ile Ala Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val145 150 155 160Gly Glu Asp Val Glu Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp
165 170 175Phe Asp Val Glu Arg Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val
180 185 190Asp Lys Ile Ser Arg Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val
195 200 205Ser Gly Glu Gly Val Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr
210 215 220Val Ala Ala Ile Pro Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp225 230 235 240Phe Ile Gln Leu Asp Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala
245 250 255Phe Ser Gly Leu Glu Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly
260 265 270Leu Gly Phe Gly Val Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile
275 280 285Val Asp Ile Phe Lys Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly
290 295 300Leu Ile Pro Glu Phe Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser305 310 315 320Asn Leu Asp Gln Lys Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn
325 330 335Ser Leu Val Lys Gln Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val
340 345 350Leu Thr Phe Thr Asp Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu
355 360 365Glu Arg Lys Thr Gly Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile
370 375 380Leu Val Pro Ile Met Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu385 390 395 400Val Ile Ile Asn Gly Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met
405 410 415Leu Glu Ala Val Ala Glu Glu Lys Thr Ala
420 425<210>71<211>1401<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1378)<223>FRXA01120<400>71acaggtaaag cgctaagatg gaacaaccca ttgccaatat tgttggttag agttgtacgc 60agtaaatctt ttcaatcgtg gaagcgggtc tcacagtcta atg gca cgt atg cag 115
Met Ala Arg Met Gln
1 5gaa agc gcc gat ctg ctc aaa tgt tcc ttc tgc gga aag agc caa aag 163Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys Gly Lys Ser Gln Lys
10 15 20cag gta aaa aaa ctc atc gcg ggt ggc gcc gta tat atc tgt gat gag 211Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val Tyr Ile Cys Asp Glu
25 30 35tgc att gag ctg tgc aac gag att att gaa gaa gaa ctc ggt caa gct 259Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu Glu Leu Gly Gln Ala
40 45 50caa cac gac gag cag gag cgc aac gag ctc ccc aag ccg tcg gag att 307Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro Lys Pro Ser Glu Ile
55 60 65tca gcc ttc ctt gat act tat gtc atc ggg cag gac cca gca aaa cgt 355Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln Asp Pro Ala Lys Arg70 75 80 85atc ctg tcg gtt gcg gtg tac aac cat tac aag cgt ctc cgc gca tcg 403Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys Arg Leu Arg Ala Ser
90 95 100gaa acc atc ggt cgt cgc agg aat gac gag cct gaa acc gaa ctg gtt 451Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro Glu Thr Glu Leu Val
105 110 115aag tcc aat att ttg atg ctc ggc ccc act ggc tcc ggc aag act ttc 499Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly Ser Gly Lys Thr Phe
120 125 130ctt gcc cag act ttg gca aag ctg ctg gat gtt cct ttt gct atc gcg 547Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val Pro Phe Ala Ile Ala
135 140 145gat gcc acc tca ctg acc gag gct ggt tat gtg ggc gag gat gtg gaa 595Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu150 155 160 165aac atc ttg ctc aag ctg ctt cag gct gct gat ttt gat gtg gaa cgt 643Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp Phe Asp Val Glu Arg
170 175 180gca cag cgc ggc atc att tac atc gat gaa gtg gac aag att tcc cgc 691Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val Asp Lys Ile Ser Arg
185 190 195aag tct gaa aac cca tcg atc act cgc gat gtt tcc ggt gaa ggc gtg 739Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val
200 205 210cag cag gca ctg ctg aaa att ttg gaa ggc act gtc gcc gca atc cca 787Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr Val Ala Ala Ile Pro
215 220 225ccg cag gga gga cgc aag cac ccc aac cag gat ttc atc cag ctg gat 835Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp Phe Ile Gln Leu Asp230 235 240 245acc acc aac att ttg ttc atc gtt gct ggt gcg ttc tct ggt ctg gag 883Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala Phe Ser Gly Leu Glu
250 255 260aag gtc atc gcg gac cgc aat ggc aag aaa ggc ttg ggc ttc ggt gtg 931Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly Leu Gly Phe Gly Val
265 270 275gag gtc tct tcc aag aag gaa gaa gcc aac att gtg gat atc ttc aag 979Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile Val Asp Ile Phe Lys
280 285 290gat gtc ctc cct gag gac ctg gtg aag ttt ggt ctc atc cca gaa ttc 1027Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly Leu Ile Pro Glu Phe
295 300 305att ggg cgt ctg cca gtc gtt gcc acc gta tcc aac ctg gat cag aaa 1075Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser Asn Leu Asp Gln Lys310 315 320 325tct ctg gtc aag gtt ctc acg gag cct cgt aac tca ttg gtg aag cag 1123Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn Ser Leu Val Lys Gln
330 335 340tat cga cgt ctg ttt gaa atg gat gac gct gtg ttg acc ttt act gat 1171Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val Leu Thr Phe Thr Asp
345 350 355gat gct ttg gag gag atc gct aat cag gca ctc gag cgc aaa act ggc 1219Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu Glu Arg Lys Thr Gly
360 365 370gcc cgt ggc ctg cgc gcg atc atg gaa gag atc ctg gtt ccg atc atg 1267Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile Leu Val Pro Ile Met
375 380 385tat gac ctc cca gac cgt aaa gac gtt ggc gaa gtc atc atc aac ggt 1315Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu Val Ile Ile Asn Gly390 395 400 405gcc gtt gcc cgt ggc gaa gcc gaa cca gag atg ttg gaa gct gtc gca 1363Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met Leu Glu Ala Val Ala
410 415 420gaa gaa aag acc gcg tagttggcag gagttatcac cgg 1401Glu Glu Lys Thr Ala
425<210>72<211>426<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>72Met Ala Arg Met Gln Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys1 5 10 15Gly Lys Ser Gln Lys Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val
20 25 30Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu
35 40 45Glu Leu Gly Gln Ala Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro
50 55 60Lys Pro Ser Glu Ile Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln65 70 75 80Asp Pro Ala Lys Arg Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys
85 90 95Arg Leu Arg Ala Ser Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro
100 105 110Glu Thr Glu Leu Val Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly
115 120 125Ser Gly Lys Thr Phe Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val
130 135 140Pro Phe Ala Ile Ala Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val145 150 155 160Gly Glu Asp Val Glu Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp
165 170 175Phe Asp Val Glu Arg Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val
180 185 190Asp Lys Ile Ser Arg Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val
195 200 205Ser Gly Glu Gly Val Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr
210 215 220Val Ala Ala Ile Pro Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp225 230 235 240Phe Ile Gln Leu Asp Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala
245 250 255Phe Ser Gly Leu Glu Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly
260 265 270Leu Gly Phe Gly Val Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile
275 280 285Val Asp Ile Phe Lys Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly
290 295 300Leu Ile Pro Glu Phe Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser305 310 315 320Asn Leu Asp Gln Lys Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn
325 330 335Ser Leu Val Lys Gln Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val
340 345 350Leu Thr Phe Thr Asp Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu
355 360 365Glu Arg Lys Thr Gly Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile
370 375 380Leu Val Pro Ile Met Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu385 390 395 400Val Ile Ile Asn Gly Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met
405 410 415Leu Glu Ala Val Ala Glu Glu Lys Thr Ala
420 425<210>73<211>1065<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1042)<223>RXA00744<400>73tctgagtcgg tagaagtatt acccagtgac ttaagtttct tagatttttt tgagcaacag 60cgaccagcca cgttagtgtg gtcgagtaga ggatagctac atg ggg aac tgg gca 115
Met Gly Asn Trp Ala
1 5gag att act gat gaa att tct aag att tac caa gat aat cag tac aag 163Glu Ile Thr Asp Glu Ile Ser Lys Ile Tyr Gln Asp Asn Gln Tyr Lys
10 15 20att aga caa ata aat gat gtt gac gca gta agc gat aaa cgt aga gaa 211Ile Arg Gln Ile Asn Asp Val Asp Ala Val Ser Asp Lys Arg Arg Glu
25 30 35gcg cta caa gca ctg ttt gaa cat act ggt cga aat gta atc gtc tat 259Ala Leu Gln Ala Leu Phe Glu His Thr Gly Arg Asn Val Ile Val Tyr
40 45 50tat tca gcg tgg tta gaa aat ggt cga cga ttt tcc ggg caa tct acg 307Tyr Ser Ala Trp Leu Glu Asn Gly Arg Arg Phe Ser Gly Gln Ser Thr
55 60 65gat ttt tcg gta aat gat act gat aaa aac agt ttt atg act gcg ctc 355Asp Phe Ser Val Asn Asp Thr Asp Lys Asn Ser Phe Met Thr Ala Leu70 75 80 85cat aag ttg gat cag agt aaa ggt ctc gat ctt atc ctc cac act ccg 403His Lys Leu Asp Gln Ser Lys Gly Leu Asp Leu Ile Leu His Thr Pro
90 95 100ggt gga gat gtt gct gcg aca gag tcg tta gta gat tac att cac gca 451Gly Gly Asp Val Ala Ala Thr Glu Ser Leu Val Asp Tyr Ile His Ala
105 110 115ctc ttt ggt caa gat ttc aga gtc att gtc ccc caa ctc gca atg tca 499Leu Phe Gly Gln Asp Phe Arg Val Ile Val Pro Gln Leu Ala Met Ser
120 125 130gca gga aca atg atc gca ctt tcg tcc aaa gag att gtt atg ggg aag 547Ala Gly Thr Met Ile Ala Leu Ser Ser Lys Glu Ile Val Met Gly Lys
135 140 145cat tct agt ctt ggc ccc att gat cct cag ttt aac ggc cta ccg gca 595His Ser Ser Leu Gly Pro Ile Asp Pro Gln Phe Asn Gly Leu Pro Ala150 155 160 165cac ggg tta ttg gaa gaa ttt gag caa gcg aag aaa gag gtc tct gag 643His Gly Leu Leu Glu Glu Phe Glu Gln Ala Lys Lys Glu Val Ser Glu
170 175 180aat ccg cag act gct cat ata tgg cag gtg atc ttg aat aaa tac aac 691Asn Pro Gln Thr Ala His Ile Trp Gln Val Ile Leu Asn Lys Tyr Asn
185 190 195ccc acg atg ttg ggt gaa gct aaa aaa gct att cag tgg tcc aac tcg 739Pro Thr Met Leu Gly Glu Ala Lys Lys Ala Ile Gln Trp Ser Asn Ser
200 205 210atg gtt aag cag tgg ctt gaa aag ggt atg ttt tta gac gag cct gac 787Met Val Lys Gln Trp Leu Glu Lys Gly Met Phe Leu Asp Glu Pro Asp
215 220 225aaa gaa gaa aaa gcc act cgc gct atc aaa gag ctc gct gat cat tcc 835Lys Glu Glu Lys Ala Thr Arg Ala Ile Lys Glu Leu Ala Asp His Ser230 235 240 245gtt act ctt gcg cat aat cga cac att tcg gtc agt aaa gca ctt gag 883Val Thr Leu Ala His Asn Arg His Ile Ser Val Ser Lys Ala Leu Glu
250 255 260ctg gga ttg aat atc aaa gaa ctt gag agc gat cca aag ctt caa gat 931Leu Gly Leu Asn Ile Lys Glu Leu Glu Ser Asp Pro Lys Leu Gln Asp
265 270 275tta gtt ctt act ctt cac cac ctg tcc gtt att gct gcg caa cga gga 979Leu Val Leu Thr Leu His His Leu Ser Val Ile Ala Ala Gln Arg Gly
280 285 290cca tta att aag ttt gtc gtc aat cat gac aac cgt ggc act ttt ctg 1027Pro Leu Ile Lys Phe Val Val Asn His Asp Asn Arg Gly Thr Phe Leu
295 300 305cag ggg cat gaa aac taattaagtg atgcaatagt cta 1065Gln Gly His Glu Asn310<210>74<211>314<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>74Met Gly Asn Trp Ala Glu Ile Thr Asp Glu Ile Ser Lys Ile Tyr Gln1 5 10 15Asp Asn Gln Tyr Lys Ile Arg Gln Ile Asn Asp Val Asp Ala Val Ser
20 25 30Asp Lys Arg Arg Glu Ala Leu Gln Ala Leu Phe Glu His Thr Gly Arg
35 40 45Asn Val Ile Val Tyr Tyr Ser Ala Trp Leu Glu Asn Gly Arg Arg Phe
50 55 60Ser Gly Gln Ser Thr Asp Phe Ser Val Asn Asp Thr Asp Lys Asn Ser65 70 75 80Phe Met Thr Ala Leu His Lys Leu Asp Gln Ser Lys Gly Leu Asp Leu
85 90 95Ile Leu His Thr Pro Gly Gly Asp Val Ala Ala Thr Glu Ser Leu Val
100 105 110Asp Tyr Ile His Ala Leu Phe Gly Gln Asp Phe Arg Val Ile Val Pro
115 120 125Gln Leu Ala Met Ser Ala Gly Thr Met Ile Ala Leu Ser Ser Lys Glu
130 135 140Ile Val Met Gly Lys His Ser Ser Leu Gly Pro Ile Asp Pro Gln Phe145 150 155 160Asn Gly Leu Pro Ala His Gly Leu Leu Glu Glu Phe Glu Gln Ala Lys
165 170 175Lys Glu Val Ser Glu Asn Pro Gln Thr Ala His Ile Trp Gln Val Ile
180 185 190Leu Asn Lys Tyr Asn Pro Thr Met Leu Gly Glu Ala Lys Lys Ala Ile
195 200 205Gln Trp Ser Asn Ser Met Val Lys Gln Trp Leu Glu Lys Gly Met Phe
210 215 220Leu Asp Glu Pro Asp Lys Glu Glu Lys Ala Thr Arg Ala Ile Lys Glu225 230 235 240Leu Ala Asp His Ser Val Thr Leu Ala His Asn Arg His Ile Ser Val
245 250 255Ser Lys Ala Leu Glu Leu Gly Leu Asn Ile Lys Glu Leu Glu Ser Asp
260 265 270Pro Lys Leu Gln Asp Leu Val Leu Thr Leu His His Leu Ser Val Ile
275 280 285Ala Ala Gln Arg Gly Pro Leu Ile Lys Phe Val Val Asn His Asp Asn
290 295 300Arg Gly Thr Phe Leu Gln Gly His Glu Asn305 310<210>75<211>957<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(934)<223>RXA00844<400>75ggtgacggcc aaaggcgcgc gcattgcgcg ccccaaaatc gacgtcattg acagcatctt 60gtgacaacat tttgtagagc aaccatctag actgttcttt atg tct tct gcg tca 115
Met Ser Ser Ala Ser
1 5ttt acc acc aaa gca ctg tcc gta ctc gca gct tta acg gct gcg tct 163Phe Thr Thr Lys Ala Leu Ser Val Leu Ala Ala Leu Thr Ala Ala Ser
10 15 20gcc ccc tta gtg gcg gcg tca cct gca cat gct ttg gca aat gct cgc 211Ala Pro Leu Val Ala Ala Ser Pro Ala His Ala Leu Ala Asn Ala Arg
25 30 35aac gtt acg ggt tca agc acc act tca gat tca att gtt cgt ctg cac 259Asn Val Thr Gly Ser Ser Thr Thr Ser Asp Ser Ile Val Arg Leu His
40 45 50atc ggt aac act gca tgt aca gga acc atg atc acc cca acg tgg gcg 307Ile Gly Asn Thr Ala Cys Thr Gly Thr Met Ile Thr Pro Thr Trp Ala
55 60 65atc acc gcc cgc cac tgt atc cct gag ggc ggt att gcc ggt gca gct 355Ile Thr Ala Arg His Cys Ile Pro Glu Gly Gly Ile Ala Gly Ala Ala70 75 80 85att ggt tca agc act ttg agc caa ttt cag cag gtg tcc caa gcg atc 403Ile Gly Ser Ser Thr Leu Ser Gln Phe Gln Gln Val Ser Gln Ala Ile
90 95 100ttg cac cct act gcg gac tta gct ctc gtt gag ctt ccc aat cag gca 451Leu His Pro Thr Ala Asp Leu Ala Leu Val Glu Leu Pro Asn Gln Ala
105 110 115agt tcc aac acg gtt gat ctc tac ggt gca cac gtg cag cct ggt gaa 499Ser Ser Asn Thr Val Asp Leu Tyr Gly Ala His Val Gln Pro Gly Glu
120 125 130aat ggt caa gca gcc ggc tgg ggt ggg tac tct gcc ttt ggc caa aat 547Asn Gly Gln Ala Ala Gly Trp Gly Gly Tyr Ser Ala Phe Gly Gln Asn
135 140 145gtt gca cag caa gcc gat gtg caa att caa cgc agg gta gtc aat gtg 595Val Ala Gln Gln Ala Asp Val Gln Ile Gln Arg Arg Val Val Asn Val150 155 160 165cca agc ccc gac cgc acc gct gtg ctg ctt gaa ggc act gtt tct aac 643Pro Ser Pro Asp Arg Thr Ala Val Leu Leu Glu Gly Thr Val Ser Asn
170 175 180ggt cgt ctc gta cca ggc gat tcc ggc gga cct ttg tac atc aat ggt 691Gly Arg Leu Val Pro Gly Asp Ser Gly Gly Pro Leu Tyr Ile Asn Gly
185 190 195caa ctg gct ggt gtg ctc agc atg tcc act gac gta gaa aac gat gca 739Gln Leu Ala Gly Val Leu Ser Met Ser Thr Asp Val Glu Asn Asp Ala
200 205 210cta gac ggc acc gtc ggc tgg tac atc ccc gtt gct gaa cac gcc gag 787Leu Asp Gly Thr Val Gly Trp Tyr Ile Pro Val Ala Glu His Ala Glu
215 220 225tgg atc gcc tac tac acc ggc aag cac att gcc ccc att gct ggt gcg 835Trp Ile Ala Tyr Tyr Thr Gly Lys His Ile Ala Pro Ile Ala Gly Ala230 235 240 245ccc gca gaa ctt gtt gac gcc acc gcc aac ccc acc ttc atc cct gct 883Pro Ala Glu Leu Val Asp Ala Thr Ala Asn Pro Thr Phe Ile Pro Ala
250 255 260cca cag cct ttc acc ggt tca tcc atc ggt ggt tgg gcg ctg ggc agc 931Pro Gln Pro Phe Thr Gly Ser Ser Ile Gly Gly Trp Ala Leu Gly Ser
265 270 275tcc tagaatatgc tgatctccct gct 957Ser<210>76<211>278<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>76Met Ser Ser Ala Ser Phe Thr Thr Lys Ala Leu Ser Val Leu Ala Ala1 5 10 15Leu Thr Ala Ala Ser Ala Pro Leu Val Ala Ala Ser Pro Ala His Ala
20 25 30Leu Ala Asn Ala Arg Asn Val Thr Gly Ser Ser Thr Thr Ser Asp Ser
35 40 45Ile Val Arg Leu His Ile Gly Asn Thr Ala Cys Thr Gly Thr Met Ile
50 55 60Thr Pro Thr Trp Ala Ile Thr Ala Arg His Cys Ile Pro Glu Gly Gly65 70 75 80Ile Ala Gly Ala Ala Ile Gly Ser Ser Thr Leu Ser Gln Phe Gln Gln
85 90 95Val Ser Gln Ala Ile Leu His Pro Thr Ala Asp Leu Ala Leu Val Glu
100 105 110Leu Pro Asn Gln Ala Ser Ser Asn Thr Val Asp Leu Tyr Gly Ala His
115 120 125Val Gln Pro Gly Glu Asn Gly Gln Ala Ala Gly Trp Gly Gly Tyr Ser
130 135 140Ala Phe Gly Gln Asn Val Ala Gln Gln Ala Asp Val Gln Ile Gln Arg145 150 155 160Arg Val Val Asn Val Pro Ser Pro Asp Arg Thr Ala Val Leu Leu Glu
165 170 175Gly Thr Val Ser Asn Gly Arg Leu Val Pro Gly Asp Ser Gly Gly Pro
180 185 190Leu Tyr Ile Asn Gly Gln Leu Ala Gly Val Leu Ser Met Ser Thr Asp
195 200 205Val Glu Asn Asp Ala Leu Asp Gly Thr Val Gly Trp Tyr Ile Pro Val
210 215 220Ala Glu His Ala Glu Trp Ile Ala Tyr Tyr Thr Gly Lys His Ile Ala225 230 235 240Pro Ile Ala Gly Ala Pro Ala Glu Leu Val Asp Ala Thr Ala Asn Pro
245 250 255Thr Phe Ile Pro Ala Pro Gln Pro Phe Thr Gly Ser Ser Ile Gly Gly
260 265 270Trp Ala Leu Gly Ser Ser
275<210>77<211>958<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(958)<223>RXA01151<400>77ccaggcgttt ggctgatggc gtggagcctc ctcgtccgca gcgtaaggca cgtcgataaa 60aagacctagt tggagggcgt aaagggttag agtggtgacc atg agt tca cca act 115
Met Ser Ser Pro Thr
1 5gat tct tcg ccc tct aat tct ttt agc gac ttc aac cgg gag gaa cag 163Asp Ser Ser Pro Ser Asn Ser Phe Ser Asp Phe Asn Arg Glu Glu Gln
10 15 20tcc cgg tta tct gat gag gtg cgc cag ctc aag cgc acc aac tct gat 211Ser Arg Leu Ser Asp Glu Val Arg Gln Leu Lys Arg Thr Asn Ser Asp
25 30 35ctt ggg gca cgt aat gcc aag ctc gcg gag atg ctg aag tcg tct cgg 259Leu Gly Ala Arg Asn Ala Lys Leu Ala Glu Met Leu Lys Ser Ser Arg
40 45 50gat aaa ttg tct gtg ctg ttt tct cag ttg gag gat atg gct cag ccg 307Asp Lys Leu Ser Val Leu Phe Ser Gln Leu Glu Asp Met Ala Gln Pro
55 60 65cca tcg gtg tat ggc act ttc ttg gaa acc gcg aaa gac ggt tct aat 355Pro Ser Val Tyr Gly Thr Phe Leu Glu Thr Ala Lys Asp Gly Ser Asn70 75 80 85gcg gag atc ttt gct ggt gga cgt cgc atg cgt gtg gct gtt tct cct 403Ala Glu Ile Phe Ala Gly Gly Arg Arg Met Arg Val Ala Val Ser Pro
90 95 100atg ctg tgt gcc gcg gat ttg atg ccg ggt gtg cag gtt cgt ttg ggt 451Met Leu Cys Ala Ala Asp Leu Met Pro Gly Val Gln Val Arg Leu Gly
105 110 115gaa ggc aat caa gtt ctt gag gcc tgt gat ttt gaa caa acc ggt gaa 499Glu Gly Asn Gln Val Leu Glu Ala Cys Asp Phe Glu Gln Thr Gly Glu
120 125 130tta gcc acg ttg atg gaa atg att ggc cgg gat cgt gct ttg gtt tca 547Leu Ala Thr Leu Met Glu Met Ile Gly Arg Asp Arg Ala Leu Val Ser
135 140 145gat cgc tcg ggg gag gag cgc gtc gtc aag ctt gct ggt ccg ttg atg 595Asp Arg Ser Gly Glu Glu Arg Val Val Lys Leu Ala Gly Pro Leu Met150 155 160 165gat cgc acc gca aag ctg ccg cgc ccc ggt gac acc ctg ctt gtt gac 643Asp Arg Thr Ala Lys Leu Pro Arg Pro Gly Asp Thr Leu Leu Val Asp
170 175 180cgc aaa gcg ggc tac gct ttt gag gcg att gcc aag acg gaa att tcg 691Arg Lys Ala Gly Tyr Ala Phe Glu Ala Ile Ala Lys Thr Glu Ile Ser
185 190 195agg ctt gcg ctg gaa gag gcg cca gat gtg tct tat cag gat att ggt 739Arg Leu Ala Leu Glu Glu Ala Pro Asp Val Ser Tyr Gln Asp Ile Gly
200 205 210ggc ttg gat gat cag att gaa ttg att caa gat gcc gtt gag ctg cca 787Gly Leu Asp Asp Gln Ile Glu Leu Ile Gln Asp Ala Val Glu Leu Pro
215 220 225ttt ttg cac ccg gag atg tac cgc gcc tac aac ctg cat cca cca aag 835Phe Leu His Pro Glu Met Tyr Arg Ala Tyr Asn Leu His Pro Pro Lys230 235 240 245ggc gtg ctg ctg tac ggc cct ccc ggc tgt gga aag acg ctg att gct 883Gly Val Leu Leu Tyr Gly Pro Pro Gly Cys Gly Lys Thr Leu Ile Ala
250 255 260aag gct gtg gct aat tct ttg gcc aac cgc atc ggt gag act ggc acc 931Lys Ala Val Ala Asn Ser Leu Ala Asn Arg Ile Gly Glu Thr Gly Thr
265 270 275tcg tac ttc atc aac gtc aag ggg cca 958Ser Tyr Phe Ile Asn Val Lys Gly Pro
280 285<210>78<211>286<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>78Met Ser Ser Pro Thr Asp Ser Ser Pro Ser Asn Ser Phe Ser Asp Phe1 5 10 15Asn Arg Glu Glu Gln Ser Arg Leu Ser Asp Glu Val Arg Gln Leu Lys
20 25 30Arg Thr Asn Ser Asp Leu Gly Ala Arg Asn Ala Lys Leu Ala Glu Met
35 40 45Leu Lys Ser Ser Arg Asp Lys Leu Ser Val Leu Phe Ser Gln Leu Glu
50 55 60Asp Met Ala Gln Pro Pro Ser Val Tyr Gly Thr Phe Leu Glu Thr Ala65 70 75 80Lys Asp Gly Ser Asn Ala Glu Ile Phe Ala Gly Gly Arg Arg Met Arg
85 90 95Val Ala Val Ser Pro Met Leu Cys Ala Ala Asp Leu Met Pro Gly Val
100 105 110Gln Val Arg Leu Gly Glu Gly Asn Gln Val Leu Glu Ala Cys Asp Phe
115 120 125Glu Gln Thr Gly Glu Leu Ala Thr Leu Met Glu Met Ile Gly Arg Asp
130 135 140Arg Ala Leu Val Ser Asp Arg Ser Gly Glu Glu Arg Val Val Lys Leu145 150 155 160Ala Gly Pro Leu Met Asp Arg Thr Ala Lys Leu Pro Arg Pro Gly Asp
165 170 175Thr Leu Leu Val Asp Arg Lys Ala Gly Tyr Ala Phe Glu Ala Ile Ala
180 185 190Lys Thr Glu Ile Ser Arg Leu Ala Leu Glu Glu Ala Pro Asp Val Ser
195 200 205Tyr Gln Asp Ile Gly Gly Leu Asp Asp Gln Ile Glu Leu Ile Gln Asp
210 215 220Ala Val Glu Leu Pro Phe Leu His Pro Glu Met Tyr Arg Ala Tyr Asn225 230 235 240Leu His Pro Pro Lys Gly Val Leu Leu Tyr Gly Pro Pro Gly Cys Gly
245 250 255Lys Thr Leu Ile Ala Lys Ala Val Ala Asn Ser Leu Ala Asn Arg Ile
260 265 270Gly Glu Thr Gly Thr Ser Tyr Phe Ile Asn Val Lys Gly Pro
275 280 285<210>79<211>735<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(712)<223>RXA02317<400>79gaattttccg gtgttaaaat gtgcggtgag cttggcgttg gcggaggacc agtgtgggga 60gacgtcgaaa agcgaattca tggccccatc ttgccttaaa atg gcg cac atg cgc 115
Met Ala His Met Arg
1 5tta ctg ctg acc tcc ttt ggc cat gat cat att cgg gat ttt gta cgc 163Leu Leu Leu Thr Ser Phe Gly His Asp His Ile Arg Asp Phe Val Arg
10 15 20ggt acc gtg gcg tat atc cct gat gcg acc agg ctt ttt gct gat agt 211Gly Thr Val Ala Tyr Ile Pro Asp Ala Thr Arg Leu Phe Ala Asp Ser
25 30 35ccc gag gct gct cct ttt atg gag acg gag cga aat atg ctg cgc gag 259Pro Glu Ala Ala Pro Phe Met Glu Thr Glu Arg Asn Met Leu Arg Glu
40 45 50cac ggc ttg agc att cgt gag ctg ccg att tcc acg tcg act ccg gag 307His Gly Leu Ser Ile Arg Glu Leu Pro Ile Ser Thr Ser Thr Pro Glu
55 60 65gaa gtg gat cgg gtg ctt ggt gag gtt gat ggg gtg tat gtg gcg ggc 355Glu Val Asp Arg Val Leu Gly Glu Val Asp Gly Val Tyr Val Ala Gly70 75 80 85ggt gag act ttt gat ctg atg tgg ctg ctg cgt tcc aca ggc aat gat 403Gly Glu Thr Phe Asp Leu Met Trp Leu Leu Arg Ser Thr Gly Asn Asp
90 95 100gag gtg ttg att aag cat gtt cgc gct ggt cta ccg tat att gga acg 451Glu Val Leu Ile Lys His Val Arg Ala Gly Leu Pro Tyr Ile Gly Thr
105 110 115agc gcc ggc gcg gta att gca ggt cct tcg att gaa ccg atc agc ttt 499Ser Ala Gly Ala Val Ile Ala Gly Pro Ser Ile Glu Pro Ile Ser Phe
120 125 130ttg gat agc ccc gat gtc gcg ccg aat tta agc gac tat tca ggt cta 547Leu Asp Ser Pro Asp Val Ala Pro Asn Leu Ser Asp Tyr Ser Gly Leu
135 140 145ggc ctg tgc gag cat gtc gtg gtg ccc cat gct ggt ggc acg atc ccg 595Gly Leu Cys Glu His Val Val Val Pro His Ala Gly Gly Thr Ile Pro150 155 160 165caa ttt ccc atc gat gtg ttt gcg gaa acc gtg cgc acc tac ggc gcc 643Gln Phe Pro Ile Asp Val Phe Ala Glu Thr Val Arg Thr Tyr Gly Ala
170 175 180gaa ttc ccg ctg gtc ctg ctt aaa gat gga cag gca ctg ctt atc gac 691Glu Phe Pro Leu Val Leu Leu Lys Asp Gly Gln Ala Leu Leu Ile Asp
185 190 195gac cac ggc gtc cac cta att taggatggtt ccccatgagc acc 735Asp His Gly Val His Leu Ile
200<210>80<211>204<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>80Met Ala His Met Arg Leu Leu Leu Thr Ser Phe Gly His Asp His Ile1 5 10 15Arg Asp Phe Val Arg Gly Thr Val Ala Tyr Ile Pro Asp Ala Thr Arg
20 25 30Leu Phe Ala Asp Ser Pro Glu Ala Ala Pro Phe Met Glu Thr Glu Arg
35 40 45Asn Met Leu Arg Glu His Gly Leu Ser Ile Arg Glu Leu Pro Ile Ser
50 55 60Thr Ser Thr Pro Glu Glu Val Asp Arg Val Leu Gly Glu Val Asp Gly65 70 75 80Val Tyr Val Ala Gly Gly Glu Thr Phe Asp Leu Met Trp Leu Leu Arg
85 90 95Ser Thr Gly Asn Asp Glu Val Leu Ile Lys His Val Arg Ala Gly Leu
100 105 110Pro Tyr Ile Gly Thr Ser Ala Gly Ala Val Ile Ala Gly Pro Ser Ile
115 120 125Glu Pro Ile Ser Phe Leu Asp Ser Pro Asp Val Ala Pro Asn Leu Ser
130 135 140Asp Tyr Ser Gly Leu Gly Leu Cys Glu His Val Val Val Pro His Ala145 150 155 160Gly Gly Thr Ile Pro Gln Phe Pro Ile Asp Val Phe Ala Glu Thr Val
165 170 175Arg Thr Tyr Gly Ala Glu Phe Pro Leu Val Leu Leu Lys Asp Gly Gln
180 185 190Ala Leu Leu Ile Asp Asp His Gly Val His Leu Ile
195 200<210>81<211>774<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(751)<223> RXA02644<400>81cgtggggaag tggagcccac actgaaaagc tttgcgaggt caacatggcc accaggctag 60tgaaaatgct gccggttggc gagctaagat gtgagatgct atg gca cat ctc acg 115
Met Ala His Leu Thr
1 5caa tac caa ctc cct cag gcc ggt caa gtc ttt gag cat gaa cta gag 163Gln Tyr Gln Leu Pro Gln Ala Gly Gln Val Phe Glu His Glu Leu Glu
10 15 20atc aag cgc tcg cga ttt ctg acc tat atc acg cgt gtg caa gat cag 211Ile Lys Arg Ser Arg Phe Leu Thr Tyr Ile Thr Arg Val Gln Asp Gln
25 30 35gag cag gct cgc gaa ttt att cac tcc atc aag gag ctg tat ccg gat 259Glu Gln Ala Arg Glu Phe Ile His Ser Ile Lys Glu Leu Tyr Pro Asp
40 45 50gcg cgt cat cat tgc agt gcc ttt att ttc cat gtg gat ggc tca aat 307Ala Arg His His Cys Ser Ala Phe Ile Phe His Val Asp Gly Ser Asn
55 60 65gat gtg gag cgt tcc tct gat gat ggc gaa cct tcc ggt acc gcg gga 355Asp Val Glu Arg Ser Ser Asp Asp Gly Glu Pro Ser Gly Thr Ala Gly70 75 80 85aaa ccc atg cta gag gcg ttg cgt ggc tcg gga atg aaa gat att gcc 403Lys Pro Met Leu Glu Ala Leu Arg Gly Ser Gly Met Lys Asp Ile Ala
90 95 100gct gtg gtg gtg cgt tat ttc ggt ggc gta aaa ctg ggc act ggc gga 451Ala Val Val Val Arg Tyr Phe Gly Gly Val Lys Leu Gly Thr Gly Gly
105 110 115tta gtt aat gcc tac acc aac gcg gtg acg gag ctt cta cct gag gtt 499Leu Val Asn Ala Tyr Thr Asn Ala Val Thr Glu Leu Leu Pro Glu Val
120 125 130ttg caa gtc acc cgc tct gtt cgg gag att ttc aag att gac ctt ccg 547Leu Gln Val Thr Arg Ser Val Arg Glu Ile Phe Lys Ile Asp Leu Pro
135 140 145cat tct gat gcg ggg cgc att gaa gcg aat ctg cgc ggc atg ggc atc 595His Ser Asp Ala Gly Arg Ile Glu Ala Asn Leu Arg Gly Met Gly Ile150 155 160 165atc att act gac act gaa tat ggt gca gaa gtc aca tac acc ttg gct 643Ile Ile Thr Asp Thr Glu Tyr Gly Ala Glu Val Thr Tyr Thr Leu Ala
170 175 180tta ttg cct ggt gaa cag gct gcg gtg gaa tct caa ttg tca tcc atg 691Leu Leu Pro Gly Glu Gln Ala Ala Val Glu Ser Gln Leu Ser Ser Met
185 190 195atg ggt gca gaa att gaa ttg aaa gaa tcc ggg cac atg tgg gtg gaa 739Met Gly Ala Glu Ile Glu Leu Lys Glu Ser Gly His Met Trp Val Glu
200 205 210tcc ccg agt gac tagtgcggtg taagagcact aga 774Ser Pro Ser Asp
215<210>82<211>217<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>82Met Ala His Leu Thr Gln Tyr Gln Leu Pro Gln Ala Gly Gln Val Phe1 5 10 15Glu His Glu Leu Glu Ile Lys Arg Ser Arg Phe Leu Thr Tyr Ile Thr
20 25 30Arg Val Gln Asp Gln Glu Gln Ala Arg Glu Phe Ile His Ser Ile Lys
35 40 45Glu Leu Tyr Pro Asp Ala Arg His His Cys Ser Ala Phe Ile Phe His
50 55 60Val Asp Gly Ser Asn Asp Val Glu Arg Ser Ser Asp Asp Gly Glu Pro65 70 75 80Ser Gly Thr Ala Gly Lys Pro Met Leu Glu Ala Leu Arg Gly Ser Gly
85 90 95Met Lys Asp Ile Ala Ala Val Val Val Arg Tyr Phe Gly Gly Val Lys
100 105 110Leu Gly Thr Gly Gly Leu Val Asn Ala Tyr Thr Asn Ala Val Thr Glu
115 120 125Leu Leu Pro Glu Val Leu Gln Val Thr Arg Ser Val Arg Glu Ile Phe
130 135 140Lys Ile Asp Leu Pro His Ser Asp Ala Gly Arg Ile Glu Ala Asn Leu145 150 155 160Arg Gly Met Gly Ile Ile Ile Thr Asp Thr Glu Tyr Gly Ala Glu Val
165 170 175Thr Tyr Thr Leu Ala Leu Leu Pro Gly Glu Gln Ala Ala Val Glu Ser
180 185 190Gln Leu Ser Ser Met Met Gly Ala Glu Ile Glu Leu Lys Glu Ser Gly
195 200 205His Met Trp Val Glu Ser Pro Ser Asp
210 215<210>83<211>1411<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1411)<223>RXN02820<400>83caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aattaaattt tctttcagaa 60aatatctccc cacataaaag ttccttgata ggctcgagag atg aaa gtg acc caa 115
Met Lys Val Thr Gln
1 5agc aca ttc ctt aaa tcg gta gct gcg ttc act gtc gca gcc tta acc 163Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr Val Ala Ala Leu Thr
10 15 20ctg acc atc tct tcg tgt tcc agc ggt gaa gac acc tcc gca agc tcc 211Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp Thr Ser Ala Ser Ser
25 30 35acg gat act gaa aac tcc tca acc caa gca gca gcg tct ccc cca ctt 259Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala Ala Ser Pro Pro Leu
40 45 50gcg cct tgt gaa ctt ccc gcc gac gct tct gct gaa gag gaa gta gaa 307Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala Glu Glu Glu Val Glu
55 60 65ggc act cac aca ggt gaa gat att tct gtt gcc ccg gaa atc ggt acc 355Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala Pro Glu Ile Gly Thr70 75 80 85ggc tac cgc gag ggc atg acc cct gtt caa acc caa ggt tat gcg gtg 403Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr Gln Gly Tyr Ala Val
90 95 100gca act gca aac ccc atc gct tct gaa gca gcc tgc gcg gtg tta aga 451Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala Cys Ala Val Leu Arg
105 110 115gaa ggc ggc act gca gct gat gct ctt gtc acc gcg cag ttt gtt ttg 499Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr Ala Gln Phe Val Leu
120 125 130gga ctg acg gaa ccg cag tcg tct ggc ctt ggt ggt ggc gga tac att 547Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly Gly Gly Gly Tyr Ile
135 140 145ctg tac tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc cgt 595Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr Ala Ile Asp Gly Arg150 155 160 165gaa aca gcg cca gtt gct gct gat gaa aac tat ctc att cat gtt tct 643Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr Leu Ile His Val Ser
170 175 180gca gag gat caa acg gca cct gtt cct gat gcc cga cgt tcc ggc agg 691Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala Arg Arg Ser Gly Arg
185 190 195tca att ggt gtg cca gga atc gtg gca gcc ctt gga cag ctg cat gat 739Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu Gly Gln Leu His Asp
200 205 210tca ttc gga aag acc tcc tgg cag gac gtg ctg aca act ccg cag cag 787Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu Thr Thr Pro Gln Gln
215 220 225ctc gca act gat ggt ttt tcc atc agc cct cgc atg tca gca tca att 835Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg Met Ser Ala Ser Ile230 235 240 245gct aac tcc gct gag gat ctc tcc cac gat ccg gaa gct gcc gca tat 883Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro Glu Ala Ala Ala Tyr
250 255 260ttc ctt gat gaa aac ggt gat gcg aag gca ccc ggc aca ctt tta caa 931Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro Gly Thr Leu Leu Gln
265 270 275aac cct gac tat gca gaa acg att cgt ctc atc tct gaa ggt ggc ccc 979Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile Ser Glu Gly Gly Pro
280 285 290gat gcg ttc tac acg ggt gag att gca gca gac atc gtg gaa cgc gcc 1027Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp Ile Val Glu Arg Ala
295 300 305acc cgt gag gtt gac ggt ttc aca cca tca ctg atg agc acg gca gat 1075Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu Met Ser Thr Ala Asp310 315 320 325ttg gct gcc tac act ccg gaa act cgt gaa gct ttg tgt gct ccc tac 1123Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala Leu Cys Ala Pro Tyr
330 335 340cgc gac aag att gtt tgt ggc atg cca ccg tca tca tcg ggt ggc gtc 1171Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser Ser Ser Gly Gly Val
345 350 355aca gtg atg gaa acc ctg ggt atc ttg aac aac ttt gat ctc gcc caa 1219Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn Phe Asp Leu Ala Gln
360 365 370tac cca ccc act gag gtt ggt ttg gat ggc gga ttg cca aat gcg gaa 1267Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly Leu Pro Asn Ala Glu
375 380 385gct gtt cac ctg att tca gag gct gag cgc ctg gct tat gct gat cgc 1315Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu Ala Tyr Ala Asp Arg390 395 400 405gat gct tac atc ggt gat cct gct ttc gtg gaa gtt cca gca ggt ggt 1363Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu Val Pro Ala Gly Gly
410 415 420gtc caa cag tgg atc aac cat gtc cac acg ggc gaa cac tcc aaa ctt 1411Val Gln Gln Trp Ile Asn His Val His Thr Gly Glu His Ser Lys Leu
425 430 435<210>84<211>437<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>84Met Lys Val Thr Gln Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr1 5 10 15Val Ala Ala Leu Thr Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp
20 25 30Thr Ser Ala Ser Ser Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala
35 40 45Ala Ser Pro Pro Leu Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala
50 55 60Glu Glu Glu Val Glu Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala65 70 75 80Pro Glu Ile Gly Thr Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr
85 90 95Gln Gly Tyr Ala Val Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala
100 105 110Cys Ala Val Leu Arg Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr
115 120 125Ala Gln Phe Val Leu Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly
130 135 140Gly Gly Gly Tyr Ile Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr145 150 155 160Ala Ile Asp Gly Arg Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr
165 170 175Leu Ile His Val Ser Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala
180 185 190Arg Arg Ser Gly Arg Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu
195 200 205Gly Gln Leu His Asp Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu
210 215 220Thr Thr Pro Gln Gln Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg225 230 235 240Met Ser Ala Ser Ile Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro
245 250 255Glu Ala Ala Ala Tyr Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro
260 265 270Gly Thr Leu Leu Gln Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile
275 280 285Ser Glu Gly Gly Pro Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp
290 295 300Ile Val Glu Arg Ala Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu305 310 315 320Met Ser Thr Ala Asp Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala
325 330 335Leu Cys Ala Pro Tyr Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser
340 345 350Ser Ser Gly Gly Val Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn
355 360 365Phe Asp Leu Ala Gln Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly
370 375 380Leu Pro Asn Ala Glu Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu385 390 395 400Ala Tyr Ala Asp Arg Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu
405 410 415Val Pro Ala Gly Gly Val Gln Gln Trp Ile Asn His Val His Thr Gly
420 425 430Glu His Ser Lys Leu
435<210>85<211>507<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(507)<223>FRXA02820<400>85gct aac tcc gct gag gat ctc tcc cac gat ccg gaa gct gcc gca tat 48Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro Glu Ala Ala Ala Tyr1 5 10 15ttc ctt gat gaa aac ggt gat gcg aag gca ccc ggc aca ctt tta caa 96Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro Gly Thr Leu Leu Gln
20 25 30aac cct gac tat gca gaa acg att cgt ctc atc tct gaa ggt ggc ccc 144Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile Ser Glu Gly Gly Pro
35 40 45gat gcg ttc tac acg ggt gag att gca gca gac atc gtg gaa cgc gcc 192Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp Ile Val Glu Arg Ala
50 55 60acc cgt gag gtt gac ggt ttc aca cca tca ctg atg agc acg gca gat 240Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu Met Ser Thr Ala Asp65 70 75 80ttg gct gcc tac act ccg gaa act cgt gaa gct ttg tgt gct ccc tac 288Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala Leu Cys Ala Pro Tyr
85 90 95cgc gac aag att gtt tgt ggc atg cca ccg tca tca tcg ggt ggc gtc 336Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser Ser Ser Gly Gly Val
100 105 110aca gtg atg gaa acc ctg ggt atc ttg aac aac ttt gat ctc gcc caa 384Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn Phe Asp Leu Ala Gln
115 120 125tac cca ccc act gag gtt ggt ttg gat ggc gga ttg cca aat gcg gaa 432Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly Leu Pro Asn Ala Glu
130 135 140gct gtt cac ctg att tca gag gct gag cgc ctg gct tat gct gat cgc 480Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu Ala Tyr Ala Asp Arg145 150 155 160gat gct tac atc ggt gat cct gct ttc 507Asp Ala Tyr Ile Gly Asp Pro Ala Phe
165<210>86<211>169<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>86Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro Glu Ala Ala Ala Tyr1 5 10 15Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro Gly Thr Leu Leu Gln
20 25 30Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile Ser Glu Gly Gly Pro
35 40 45Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp Ile Val Glu Arg Ala
50 55 60Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu Met Ser Thr Ala Asp65 70 75 80Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala Leu Cys Ala Pro Tyr
85 90 95Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser Ser Ser Gly Gly Val
100 105 110Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn Phe Asp Leu Ala Gln
115 120 125Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly Leu Pro Asn Ala Glu
130 135 140Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu Ala Tyr Ala Asp Arg145 150 155 160Asp Ala Tyr Ile Gly Asp Pro Ala Phe
165<210>87<211>604<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(604)<223>FRXA02000<400>87caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aattaaattt tctttcagaa 60aatatctccc cacataaaag ttccttgata ggctcgagag atg aaa gtg acc caa 115
Met Lys Val Thr Gln
1 5agc aca ttc ctt aaa tcg gta gct gcg ttc act gtc gca gcc tta acc 163Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr Val Ala Ala Leu Thr
10 15 20ctg acc atc tct tcg tgt tcc agc ggt gaa gac acc tcc gca agc tcc 211Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp Thr Ser Ala Ser Ser
25 30 35acg gat act gaa aac tcc tca acc caa gca gca gcg tct ccc cca ctt 259Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala Ala Ser Pro Pro Leu
40 45 50gcg cct tgt gaa ctt ccc gcc gac gct tct gct gaa gag gaa gta gaa 307Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala Glu Glu Glu Val Glu
55 60 65ggc act cac aca ggt gaa gat att tct gtt gcc ccg gaa atc ggt acc 355Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala Pro Glu Ile Gly Thr70 75 80 85ggc tac cgc gag ggc atg acc cct gtt caa acc caa ggt tat gcg gtg 403Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr Gln Gly Tyr Ala Val
90 95 100gca act gca aac ccc atc gct tct gaa gca gcc tgc gcg gtg tta aga 451Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala Cys Ala Val Leu Arg
105 110 115gaa ggc ggc act gca gct gat gct ctt gtc acc gcg cag ttt gtt ttg 499Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr Ala Gln Phe Val Leu
120 125 130gga ctg acg gaa ccg cag tcg tct ggc ctt ggt ggt ggc gga tac att 547Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly Gly Gly Gly Tyr Ile
135 140 145ctg tac tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc cgt 595Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr Ala Ile Asp Gly Arg150 155 160 165gaa aca gcg 604Glu Thr Ala<210>88<211>168<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>88Met Lys Val Thr Gln Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr1 5 10 15Val Ala Ala Leu Thr Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp
20 25 30Thr Ser Ala Ser Ser Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala
35 40 45Ala Ser Pro Pro Leu Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala
50 55 60Glu Glu Glu Val Glu Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala65 70 75 80Pro Glu lle Gly Thr Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr
85 90 95Gln Gly Tyr Ala Val Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala
100 105 110Cys Ala Val Leu Arg Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr
115 120 125Ala Gln Phe Val Leu Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly
130 135 140Gly Gly Gly Tyr Ile Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr145 150 155 160Ala Ile Asp Gly Arg Glu Thr Ala
165<210>89<211>824<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(801)<223>RXN03178<400>89ccc acc acc gtg gtc acg ggc acg atg gaa gcc gcc aac atc gag ggc 48Pro Thr Thr Val Val Thr Gly Thr Met Glu Ala Ala Asn Ile Glu Gly1 5 10 15tcc cgc gtg ggt gtc ggc gag gcc ggc cag tat acc gtt gat cag ctg 96Ser Arg Val Gly Val Gly Glu Ala Gly Gln Tyr Thr Val Asp Gln Leu
20 25 30ctg cac ggt ctt ctt tta gcc agc ggt aac gat gcg gcg tat atg ttg 144Leu His Gly Leu Leu Leu Ala Ser Gly Asn Asp Ala Ala Tyr Met Leu
35 40 45gct cag gaa ctt ggt ggg gat caa gca acc ctg gag aaa gta aac gcg 192Ala Gln Glu Leu Gly Gly Asp Gln Ala Thr Leu Glu Lys Val Asn Ala
50 55 60ctg gcc aag gag ttg ggc act caa gac acc ttc gtt gcc act tat tcc 240Leu Ala Lys Glu Leu Gly Thr Gln Asp Thr Phe Val Ala Thr Tyr Ser65 70 75 80ggt ttg gat gcg ccg gga atg tcg acc tcc gca tac gac atg tca ttg 288Gly Leu Asp Ala Pro Gly Met Ser Thr Ser Ala Tyr Asp Met Ser Leu
85 90 95att tat cag cat gcg tgg cag aac ccg gtc ttc gag tcg att atc tcc 336Ile Tyr Gln His Ala Trp Gln Asn Pro Val Phe Glu Ser Ile Ile Ser
100 105 110acc gat cac att gat ttc cct ggt tgg ggc gac aat gag ggt ttc caa 384Thr Asp His Ile Asp Phe Pro Gly Trp Gly Asp Asn Glu Gly Phe Gln
115 120 125gtc tgg aac gat aac gcc ttg ttc atg aac gat cct gat ggc atc ggc 432Val Trp Asn Asp Asn Ala Leu Phe Met Asn Asp Pro Asp Gly Ile Gly
130 135 140ggc aag acc ggc tac acc gac gac gcg aac cac acc ttt gtc ggc ggt 480Gly Lys Thr Gly Tyr Thr Asp Asp Ala Asn His Thr Phe Val Gly Gly145 150 155 160ctc gat cgg ggt ggt cgc cgc ctc gcc gcc gta ctc ttg gat tcc acc 528Leu Asp Arg Gly Gly Arg Arg Leu Ala Ala Val Leu Leu Asp Ser Thr
165 170 175gtc agc gac att cgt ccg tgg gaa caa gca cga ttg ctt atc gac gcc 576Val Ser Asp Ile Arg Pro Trp Glu Gln Ala Arg Leu Leu Ile Asp Ala
180 185 190tcc ctc ccc atc acg ccg ggg tcc ggc gtg ggc cag ctg ggc tcc ggc 624Ser Leu Pro Ile Thr Pro Gly Ser Gly Val Gly Gln Leu Gly Ser Gly
195 200 205agc gcg aac gat gtg gca ccg gcg acc cca gaa tta cca gaa ccc acc 672Ser Ala Asn Asp Val Ala Pro Ala Thr Pro Glu Leu Pro Glu Pro Thr
210 215 220gac aac ctg act tca ggt gag ggt ggg tcg cag aac acg ctt ctt aag 720Asp Asn Leu Thr Ser Gly Glu Gly Gly Ser Gln Asn Thr Leu Leu Lys225 230 235 240ctc gtg gtg ccc atc gga atc atc gtg ctg ttg cta atc gcc gca cta 768Leu Val Val Pro Ile Gly Ile Ile Val Leu Leu Leu Ile Ala Ala Leu
245 250 255gcg tgg aca ttc aga tct ccc aag aaa aag aac taggtgttct tcttcacgac 821Ala Trp Thr Phe Arg Ser Pro Lys Lys Lys Asn
260 265ctc 824<210>90<211>267<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>90Pro Thr Thr Val Val Thr Gly Thr Met Glu Ala Ala Asn Ile Glu Gly1 5 10 15Ser Arg Val Gly Val Gly Glu Ala Gly Gln Tyr Thr Val Asp Gln Leu
20 25 30Leu His Gly Leu Leu Leu Ala Ser Gly Asn Asp Ala Ala Tyr Met Leu
35 40 45Ala Gln Glu Leu Gly Gly Asp Gln Ala Thr Leu Glu Lys Val Asn Ala
50 55 60Leu Ala Lys Glu Leu Gly Thr Gln Asp Thr Phe Val Ala Thr Tyr Ser65 70 75 80Gly Leu Asp Ala Pro Gly Met Ser Thr Ser Ala Tyr Asp Met Ser Leu
85 90 95Ile Tyr Gln His Ala Trp Gln Asn Pro Val Phe Glu Ser Ile Ile Ser
100 105 110Thr Asp His Ile Asp Phe Pro Gly Trp Gly Asp Asn Glu Gly Phe Gln
115 120 125Val Trp Asn Asp Asn Ala Leu Phe Met Asn Asp Pro Asp Gly Ile Gly
130 135 140Gly Lys Thr Gly Tyr Thr Asp Asp Ala Asn His Thr Phe Val Gly Gly145 150 155 160Leu Asp Arg Gly Gly Arg Arg Leu Ala Ala Val Leu Leu Asp Ser Thr
165 170 175Val Ser Asp Ile Arg Pro Trp Glu Gln Ala Arg Leu Leu Ile Asp Ala
180 185 190Ser Leu Pro Ile Thr Pro Gly Ser Gly Val Gly Gln Leu Gly Ser Gly
195 200 205Ser Ala Asn Asp Val Ala Pro Ala Thr Pro Glu Leu Pro Glu Pro Thr
210 215 220Asp Asn Leu Thr Ser Gly Glu Gly Gly Ser Gln Asn Thr Leu Leu Lys225 230 235 240Leu Val Val Pro Ile Gly Ile Ile Val Leu Leu Leu Ile Ala Ala Leu
245 250 255Ala Trp Thr Phe Arg Ser Pro Lys Lys Lys Asn
260 265<210>91<211>749<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(726)<223>FRXA02859<400>91cag tat acc gtt gat cag ctg ctg cac ggt ctt ctt tta gcc agc ggt 48Gln Tyr Thr Val Asp Gln Leu Leu His Gly Leu Leu Leu Ala Ser Gly1 5 10 15aac gat gcg gcg tat ctg ttg gct cag gaa ctt ggt ggg gat caa gca 96Asn Asp Ala Ala Tyr Leu Leu Ala Gln Glu Leu Gly Gly Asp Gln Ala
20 25 30acc ctg gag aaa gta aac gcg ctg gcc aag gag ttg ggc act caa gac 144Thr Leu Glu Lys Val Asn Ala Leu Ala Lys Glu Leu Gly Thr Gln Asp
35 40 45acc ttc gtt gcc act tat tcc ggt ttg gat gcg ccg gga atg tcg acc 192Thr Phe Val Ala Thr Tyr Ser Gly Leu Asp Ala Pro Gly Met Ser Thr
50 55 60tcc gca tac gac atg tca ttg att tat cag cat gcg tgg cag aac ccg 240Ser Ala Tyr Asp Met Ser Leu Ile Tyr Gln His Ala Trp Gln Asn Pro65 70 75 80gtt ttc gag tcg att atc tcc acc gat cac att gat ttc cct ggt tgg 288Val Phe Glu Ser Ile Ile Ser Thr Asp His Ile Asp Phe Pro Gly Trp
85 90 95ggc gac aat gag ggt ttc caa gtc tgg aac gat aac gcc ttg ttc atg 336Gly Asp Asn Glu Gly Phe Gln Val Trp Asn Asp Asn Ala Leu Phe Met
100 105 110aac gat cct gat ggc atc ggc ggc aag acc ggc tac acc gac gac gcg 384Asn Asp Pro Asp Gly Ile Gly Gly Lys Thr Gly Tyr Thr Asp Asp Ala
115 120 125aac cac acc ttt gtc ggc ggt ctc gat cgg ggt ggt cgc cgc ctc gcc 432Asn His Thr Phe Val Gly Gly Leu Asp Arg Gly Gly Arg Arg Leu Ala
130 135 140gcc gta ctc ttg gat tcc acc gtc agc gac att cgt ccg tgg gaa caa 480Ala Val Leu Leu Asp Ser Thr Val Ser Asp Ile Arg Pro Trp Glu Gln145 150 155 160gca cga ttg ctt atc gac gcc tcc ctc ccc atc acg ccg ggg tcc ggc 528Ala Arg Leu Leu Ile Asp Ala Ser Leu Pro Ile Thr Pro Gly Ser Gly
165 170 175gtg ggc cag ctg ggc tcc ggc agc gcg aac gat gtg gca ccg gcg acc 576Val Gly Gln Leu Gly Ser Gly Ser Ala Asn Asp Val Ala Pro Ala Thr
180 185 190cca gaa tta cca gaa ccc acc gac aac ctg act tca ggt gag ggt ggg 624Pro Glu Leu Pro Glu Pro Thr Asp Asn Leu Thr Ser Gly Glu Gly Gly
195 200 205tcg cag aac acg ctg ctt aag ctc gtg gtg ccc atc gga atc atc gtg 672Ser Gln Asn Thr Leu Leu Lys Leu Val Val Pro Ile Gly Ile Ile Val
210 215 220ctg ttg cta atc gcc gca cta gcg tgg aca ttc aga tct ccc aag aaa 720Leu Leu Leu Ile Ala Ala Leu Ala Trp Thr Phe Arg Ser Pro Lys Lys225 230 235 240aag aac taggtgttct tcttcacgac ctc 749Lys Asn<210>92<211>242<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>92Gln Tyr Thr Val Asp Gln Leu Leu His Gly Leu Leu Leu Ala Ser Gly1 5 10 15Asn Asp Ala Ala Tyr Leu Leu Ala Gln Glu Leu Gly Gly Asp Gln Ala
20 25 30Thr Leu Glu Lys Val Asn Ala Leu Ala Lys Glu Leu Gly Thr Gln Asp
35 40 45Thr Phe Val Ala Thr Tyr Ser Gly Leu Asp Ala Pro Gly Met Ser Thr
50 55 60Ser Ala Tyr Asp Met Ser Leu Ile Tyr Gln His Ala Trp Gln Asn Pro65 70 75 80Val Phe Glu Ser Ile Ile Ser Thr Asp His Ile Asp Phe Pro Gly Trp
85 90 95Gly Asp Asn Glu Gly Phe Gln Val Trp Asn Asp Asn Ala Leu Phe Met
100 105 110Asn Asp Pro Asp Gly Ile Gly Gly Lys Thr Gly Tyr Thr Asp Asp Ala
115 120 125Asn His Thr Phe Val Gly Gly Leu Asp Arg Gly Gly Arg Arg Leu Ala
130 135 140Ala Val Leu Leu Asp Ser Thr Val Ser Asp Ile Arg Pro Trp Glu Gln145 150 155 160Ala Arg Leu Leu Ile Asp Ala Ser Leu Pro Ile Thr Pro Gly Ser Gly
165 170 175Val Gly Gln Leu Gly Ser Gly Ser Ala Asn Asp Val Ala Pro Ala Thr
180 185 190Pro Glu Leu Pro Glu Pro Thr Asp Asn Leu Thr Ser Gly Glu Gly Gly
195 200 205Ser Gln Asn Thr Leu Leu Lys Leu Val Val Pro Ile Gly Ile Ile Val
210 215 220Leu Leu Leu Ile Ala Ala Leu Ala Trp Thr Phe Arg Ser Pro Lys Lys225 230 235 240Lys Asn<210>93<211>1212<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1189)<223>RXA00137<400>93tttacagagt gcttatgagg caatcagcca ctaagtgttg agtaatctac tagtttggac 60tagaagttac ccactttcag tgaattttta aggagagaac atg gct ttg gct gat 115
Met Ala Leu Ala Asp
l 5acc cga ttt gcc act cgt cgt cgc gca ctt gcc gca aaa ctg gca gct 163Thr Arg Phe Ala Thr Arg Arg Arg Ala Leu Ala Ala Lys Leu Ala Ala
10 15 20caa cgg atc gac tca att ttg gtg aca agc ccg atc cat gtt cgc tat 211Gln Arg Ile Asp Ser Ile Leu Val Thr Ser Pro Ile His Val Arg Tyr
25 30 35ctc agc gga ttc acc ggc tcc aac ggc gca ctg atc gtg aac aaa gat 259Leu Ser Gly Phe Thr Gly Ser Asn Gly Ala Leu Ile Val Asn Lys Asp
40 45 50ctc tcc gcg cag atc tgc acc gac ggt cgc tac acc acc cag atc gca 307Leu Ser Ala Gln Ile Cys Thr Asp Gly Arg Tyr Thr Thr Gln Ile Ala
55 60 65gaa gaa gtc ccg gac atc gag gcg ctg att gag cgt gcc tcg gca acg 355Glu Glu Val Pro Asp Ile Glu Ala Leu Ile Glu Arg Ala Ser Ala Thr70 75 80 85acg ctg cta gcg cag gtc gaa ggg ccg cgt cgt ata gca atc gaa gcc 403Thr Leu Leu Ala Gln Val Glu Gly Pro Arg Arg Ile Ala Ile Glu Ala
90 95 100gca caa acc acc ctg gac cag cta gac agc ctg cgt gaa gca acc cag 451Ala Gln Thr Thr Leu Asp Gln Leu Asp Ser Leu Arg Glu Ala Thr Gln
105 110 115gaa gac gtc gag ctg atc ccc gtg tca ggt gtt gtg gaa tcc att cgc 499Glu Asp Val Glu Leu Ile Pro Val Ser Gly Val Val Glu Ser Ile Arg
120 125 130ctg acc aaa gac agc ttc gaa ctc gac cgc ctc cgc gat gtc gca gcg 547Leu Thr Lys Asp Ser Phe Glu Leu Asp Arg Leu Arg Asp Val Ala Ala
135 140 145ctg gct tcc caa gca ttc gaa gat tta ctc gca gca gga gaa ctc gcc 595Leu Ala Ser Gln Ala Phe Glu Asp Leu Leu Ala Ala Gly Glu Leu Ala150 155 160 165gaa ggc cga tca gag cgc caa gtc gcc gcc gat ctg gaa tac cgc atg 643Glu Gly Arg Ser Glu Arg Gln Val Ala Ala Asp Leu Glu Tyr Arg Met
170 175 180cgc ctg ttg gga gca gaa cgc ccc agc ttc gac acc atc gtg gcc tct 691Arg Leu Leu Gly Ala Glu Arg Pro Ser Phe Asp Thr Ile Val Ala Ser
185 190 195gga cct aac tcc gcg aaa cca cac cac ggc gca ggc gac cgc atc ctc 739Gly Pro Asn Ser Ala Lys Pro His His Gly Ala Gly Asp Arg Ile Leu
200 205 210cag cgc ggc gat cta gtc acc atc gat ttc ggc gca cac gca cgc gga 787Gln Arg Gly Asp Leu Val Thr Ile Asp Phe Gly Ala His Ala Arg Gly
215 220 225ttc aac tcc gat atg acc cgc acc ctc gtt atg ggc gaa gca ggg gag 835Phe Asn Ser Asp Met Thr Arg Thr Leu Val Met Gly Glu Ala Gly Glu230 235 240 245ttc gaa gca gaa atc tac gac atc gtc ctg cgc tcc caa ctc gct ggt 883Phe Glu Ala Glu Ile Tyr Asp Ile Val Leu Arg Ser Gln Leu Ala Gly
250 255 260gtt gaa gca gcc tac tca ggc gcc aac ctc ttc gac atc gac gca gca 931Val Glu Ala Ala Tyr Ser Gly Ala Asn Leu Phe Asp Ile Asp Ala Ala
265 270 275tgc cgc aaa atc atc gaa gac gca ggc tac ggc gaa tac ttc gtg cac 979Cys Arg Lys Ile Ile Glu Asp Ala Gly Tyr Gly Glu Tyr Phe Val His
280 285 290tcc acc ggc cac ggc atc gga ctt gaa gtc cac gaa gcc cca agc gca 1027Ser Thr Gly His Gly Ile Gly Leu Glu Val His Glu Ala Pro Ser Ala
295 300 305tcc aaa acc tca caa gga gtc cta gaa acc ggc tcc aca ctg acc atc 1075Ser Lys Thr Ser Gln Gly Val Leu Glu Thr Gly Ser Thr Leu Thr Ile310 315 320 325gaa ccc gga att tac gtc ccc gga aag ggc ggc gta cgc atc gaa gac 1123Glu Pro Gly Ile Tyr Val Pro Gly Lys Gly Gly Val Arg Ile Glu Asp
330 335 340acc ctg att att acc tca gga gca ccg gaa atc atc acc aag gtg agt 1171Thr Leu Ile Ile Thr Ser Gly Ala Pro Glu Ile Ile Thr Lys Val Ser
345 350 355aag gac ctc atc gtg gtg taatctaggt gagctaatcg gtc 1212Lys Asp Leu Ile Val Val
360<210>94<211>363<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>94Met Ala Leu Ala Asp Thr Arg Phe Ala Thr Arg Arg Arg Ala Leu Ala1 5 10 15Ala Lys Leu Ala Ala Gln Arg Ile Asp Ser Ile Leu Val Thr Ser Pro
20 25 30Ile His Val Arg Tyr Leu Ser Gly Phe Thr Gly Ser Asn Gly Ala Leu
35 40 45Ile Val Asn Lys Asp Leu Ser Ala Gln Ile Cys Thr Asp Gly Arg Tyr
50 55 60Thr Thr Gln Ile Ala Glu Glu Val Pro Asp Ile Glu Ala Leu Ile Glu65 70 75 80Arg Ala Ser Ala Thr Thr Leu Leu Ala Gln Val Glu Gly Pro Arg Arg
85 90 95Ile Ala Ile Glu Ala Ala Gln Thr Thr Leu Asp Gln Leu Asp Ser Leu
100 105 110Arg Glu Ala Thr Gln Glu Asp Val Glu Leu Ile Pro Val Ser Gly Val
115 120 125Val Glu Ser Ile Arg Leu Thr Lys Asp Ser Phe Glu Leu Asp Arg Leu
130 135 140Arg Asp Val Ala Ala Leu Ala Ser Gln Ala Phe Glu Asp Leu Leu Ala145 150 155 160Ala Gly Glu Leu Ala Glu Gly Arg Ser Glu Arg Gln Val Ala Ala Asp
165 170 175Leu Glu Tyr Arg Met Arg Leu Leu Gly Ala Glu Arg Pro Ser Phe Asp
180 185 190Thr Ile Val Ala Ser Gly Pro Asn Ser Ala Lys Pro His His Gly Ala
195 200 205Gly Asp Arg Ile Leu Gln Arg Gly Asp Leu Val Thr Ile Asp Phe Gly
210 215 220Ala His Ala Arg Gly Phe Asn Ser Asp Met Thr Arg Thr Leu Val Met225 230 235 240Gly Glu Ala Gly Glu Phe Glu Ala Glu Ile Tyr Asp Ile Val Leu Arg
245 250 255Ser Gln Leu Ala Gly Val Glu Ala Ala Tyr Ser Gly Ala Asn Leu Phe
260 265 270Asp Ile Asp Ala Ala Cys Arg Lys Ile Ile Glu Asp Ala Gly Tyr Gly
275 280 285Glu Tyr Phe Val His Ser Thr Gly His Gly Ile Gly Leu Glu Val His
290 295 300Glu Ala Pro Ser Ala Ser Lys Thr Ser Gln Gly Val Leu Glu Thr Gly305 310 315 320Ser Thr Leu Thr Ile Glu Pro Gly Ile Tyr Val Pro Gly Lys Gly Gly
325 330 335Val Arg Ile Glu Asp Thr Leu Ile Ile Thr Ser Gly Ala Pro Glu Ile
340 345 350Ile Thr Lys Val Ser Lys Asp Leu Ile Val Val
355 360<210>95<211>1404<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1381)<223>RXN00499<400>95tgccaacagg ggatatgcca ctgtgtaccc cacggcgatg atcggtaaaa tcctcggcgc 60gcagatattg ttcttgctgc tctaaggtga tttttgggca gtg gtg ggg gtg gtg 115
Val Val Gly Val Val
1 5tcc acc cct gcg cgt aac ctg gga agc atg act aaa aca ctt ggt tcc 163Ser Thr Pro Ala Arg Asn Leu Gly Ser Met Thr Lys Thr Leu Gly Ser
10 15 20ctt cag ctg gaa gaa atc acg ctg acc ctc cct ctg act gaa gat gtg 211Leu Gln Leu Glu Glu Ile Thr Leu Thr Leu Pro Leu Thr Glu Asp Val
25 30 35gcc gat gaa cgc acc att gat gtg ttc gca cgc att gcc aca cgc gtc 259Ala Asp Glu Arg Thr Ile Asp Val Phe Ala Arg Ile Ala Thr Arg Val
40 45 50ggt ggg gaa gac ctt cca tat tta gta ttc ctg cag ggt ggg cct ggc 307Gly Gly Glu Asp Leu Pro Tyr Leu Val Phe Leu Gln Gly Gly Pro Gly
55 60 65aat gaa gct cca cgt cca agc ctt aat ccc ctc aac ccc aat tgg ttg 355Asn Glu Ala Pro Arg Pro Ser Leu Asn Pro Leu Asn Pro Asn Trp Leu70 75 80 85ggc gtg gcc ttg gag gaa tac cgc gtg gtc atg ttg gat caa cgt ggc 403Gly Val Ala Leu Glu Glu Tyr Arg Val Val Met Leu Asp Gln Arg Gly
90 95 100acc ggc cgt tcc acc cca gtg ggt aat gat att ttg gaa aaa ccc aca 451Thr Gly Arg Ser Thr Pro Val Gly Asn Asp Ile Leu Glu Lys Pro Thr
105 110 115gca gaa gta gtg gag tac tta tcc cac ctg cgc gca gat ggc att gtg 499Ala Glu Val Val Glu Tyr Leu Ser His Leu Arg Ala Asp Gly Ile Val
120 125 130cga gat gct gaa gcc ctg cgt aag cat ttg ggt gtg aat cag tgg aac 547Arg Asp Ala Glu Ala Leu Arg Lys His Leu Gly Val Asn Gln Trp Asn
135 140 145ctt tta ggc cag tcc ttc gga ggt ttc acc acc ctg cat tac ttg tcc 595Leu Leu Gly Gln Ser Phe Gly Gly Phe Thr Thr Leu His Tyr Leu Ser150 155 160 165cgg cac gcc gat tcc ttg gac aac gtg ttt att acc ggc ggt ctc agc 643Arg His Ala Asp Ser Leu Asp Asn Val Phe Ile Thr Gly Gly Leu Ser
170 175 180gct att gat cgc cca gca gaa gac gtg tat gcc aac tgt tac aac cgc 691Ala Ile Asp Arg Pro Ala Glu Asp Val Tyr Ala Asn Cys Tyr Asn Arg
185 190 195atg cgc cga aac tct gag gaa ttc tac cgt cgc ttc ccg caa tta cgg 739Met Arg Arg Asn Ser Glu Glu Phe Tyr Arg Arg Phe Pro Gln Leu Arg
200 205 210gaa act ttc cga ggg ttg gtt aat cgt gct cgc gcc ggg gag att gtg 787Glu Thr Phe Arg Gly Leu Val Asn Arg Ala Arg Ala Gly Glu Ile Val
215 220 225ctt ccc acc ggc gaa gtt gtg tca gaa acc agg ctg cga tcc ctt ggt 835Leu Pro Thr Gly Glu Val Val Ser Glu Thr Arg Leu Arg Ser Leu Gly230 235 240 245cac ttg ttg ggt agc aat gac ggc tgg ttt gat ctg tac aac ctg ctg 883His Leu Leu Gly Ser Asn Asp Gly Trp Phe Asp Leu Tyr Asn Leu Leu
250 255 260gaa tta gat ccc acc tcc aac gct ttt gtc cat gac ctg gca gga ctt 931Glu Leu Asp Pro Thr Ser Asn Ala Phe Val His Asp Leu Ala Gly Leu
265 270 275ttg cct ttc ggc aac cgc aac cca att tat tac gtg ctc cat gag tcc 979Leu Pro Phe Gly Asn Arg Asn Pro Ile Tyr Tyr Val Leu His Glu Ser
280 285 290tct tac gcc gac ggt gtg gtg aca aat tgg gca gca gag cgt gtg ctt 1027Ser Tyr Ala Asp Gly Val Val Thr Asn Trp Ala Ala Glu Arg Val Leu
295 300 305cca gag gat ttc cgc gag gat cca aca ctg ctc acc ggt gag cac gtg 1075Pro Glu Asp Phe Arg Glu Asp Pro Thr Leu Leu Thr Gly Glu His Val310 315 320 325ttc cag gag tgg aca gac acc gtg ccg tcg ctc aag ccg tgg aag gac 1123Phe Gln Glu Trp Thr Asp Thr Val Pro Ser Leu Lys Pro Trp Lys Asp
330 335 340gtt gcc ctg gca ttg gct cag cag gaa tgg ccc aag ctt tat gat gcg 1171Val Ala Leu Ala Leu Ala Gln Gln Glu Trp Pro Lys Leu Tyr Asp Ala
345 350 355aag gca ttg gaa aac tca cag gcc aag ggc gct gca gca gtg tat gcc 1219Lys Ala Leu Glu Asn Ser Gln Ala Lys Gly Ala Ala Ala Val Tyr Ala
360 365 370aat gac gtt ttc gtc cca gtg gat tac tct ctg gaa acc gca caa cac 1267Asn Asp Val Phe Val Pro Val Asp Tyr Ser Leu Glu Thr Ala Gln His
375 380 385ctg ccc ggt gtg cag ctg ttt atc acc agc cag cat gaa cac aat gga 1315Leu Pro Gly Val Gln Leu Phe Ile Thr Ser Gln His Glu His Asn Gly390 395 400 405ctt cgt gcc agc tca ggc gca gta ctg aag cac ctt ttc gat ctg gcc 1363Leu Arg Ala Ser Ser Gly Ala Val Leu Lys His Leu Phe Asp Leu Ala
410 415 420cac ggc cga gag gta cgc tgattcctcg tgttagtact agc 1404His Gly Arg Glu Val Arg
425<210>96<211>427<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>96Val Val Gly Val Val Ser Thr Pro Ala Arg Asn Leu Gly Ser Met Thr1 5 10 15Lys Thr Leu Gly Ser Leu Gln Leu Glu Glu Ile Thr Leu Thr Leu Pro
20 25 30Leu Thr Glu Asp Val Ala Asp Glu Arg Thr Ile Asp Val Phe Ala Arg
35 40 45Ile Ala Thr Arg Val Gly Gly Glu Asp Leu Pro Tyr Leu Val Phe Leu
50 55 60Gln Gly Gly Pro Gly Asn Glu Ala Pro Arg Pro Ser Leu Asn Pro Leu65 70 75 80Asn Pro Asn Trp Leu Gly Val Ala Leu Glu Glu Tyr Arg Val Val Met
85 90 95Leu Asp Gln Arg Gly Thr Gly Arg Ser Thr Pro Val Gly Asn Asp Ile
100 105 110Leu Glu Lys Pro Thr Ala Glu Val Val Glu Tyr Leu Ser His Leu Arg
115 120 125Ala Asp Gly Ile Val Arg Asp Ala Glu Ala Leu Arg Lys His Leu Gly
130 135 140Val Asn Gln Trp Asn Leu Leu Gly Gln Ser Phe Gly Gly Phe Thr Thr145 150 155 160Leu His Tyr Leu Ser Arg His Ala Asp Ser Leu Asp Asn Val Phe Ile
165 170 175Thr Gly Gly Leu Ser Ala Ile Asp Arg Pro Ala Glu Asp Val Tyr Ala
180 185 190Asn Cys Tyr Asn Arg Met Arg Arg Asn Ser Glu Glu Phe Tyr Arg Arg
195 200 205Phe Pro Gln Leu Arg Glu Thr Phe Arg Gly Leu Val Asn Arg Ala Arg
210 215 220Ala Gly Glu Ile Val Leu Pro Thr Gly Glu Val Val Ser Glu Thr Arg225 230 235 240Leu Arg Ser Leu Gly His Leu Leu Gly Ser Asn Asp Gly Trp Phe Asp
245 250 255Leu Tyr Asn Leu Leu Glu Leu Asp Pro Thr Ser Asn Ala Phe Val His
260 265 270Asp Leu Ala Gly Leu Leu Pro Phe Gly Asn Arg Asn Pro Ile Tyr Tyr
275 280 285Val Leu His Glu Ser Ser Tyr Ala Asp Gly Val Val Thr Asn Trp Ala
290 295 300Ala Glu Arg Val Leu Pro Glu Asp Phe Arg Glu Asp Pro Thr Leu Leu305 310 315 320Thr Gly Glu His Val Phe Gln Glu Trp Thr Asp Thr Val Pro Ser Leu
325 330 335Lys Pro Trp Lys Asp Val Ala Leu Ala Leu Ala Gln Gln Glu Trp Pro
340 345 350Lys Leu Tyr Asp Ala Lys Ala Leu Glu Asn Ser Gln Ala Lys Gly Ala
355 360 365Ala Ala Val Tyr Ala Asn Asp Val Phe Val Pro Val Asp Tyr Ser Leu
370 375 380Glu Thr Ala Gln His Leu Pro Gly Val Gln Leu Phe Ile Thr Ser Gln385 390 395 400His Glu His Asn Gly Leu Arg Ala Ser Ser Gly Ala Val Leu Lys His
405 410 415Leu Phe Asp Leu Ala His Gly Arg Glu Val Arg
420 425<210> 97<211>980<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(957)<223>FRXA00499<400>97ggt aat gat att ttg gaa aaa ccc aca gca gaa gta gtg gag tac tta 48Gly Asn Asp Ile Leu Glu Lys Pro Thr Ala Glu Val Val Glu Tyr Leu1 5 10 15tcc cac ctg cgc gca gat ggc att gtg cga gat gct gaa gcc ctg cgt 96Ser His Leu Arg Ala Asp Gly Ile Val Arg Asp Ala Glu Ala Leu Arg
20 25 30aag cat ttg ggt gtg aat cag tgg aac ctt tta ggc cag tcc ttc gga 144Lys His Leu Gly Val Asn Gln Trp Asn Leu Leu Gly Gln Ser Phe Gly
35 40 45ggt ttc acc acc ctg cat tac ttg tcc cgg cac gcc gat tcc ttg gac 192Gly Phe Thr Thr Leu His Tyr Leu Ser Arg His Ala Asp Ser Leu Asp
50 55 60aac gtg ttt att acc ggc ggt ctc agc gct att gat cgc cca gca gaa 240Asn Val Phe Ile Thr Gly Gly Leu Ser Ala Ile Asp Arg Pro Ala Glu65 70 75 80gac gtg tat gcc aac tgt tac aac cgc atg cgc cga aac tct gag gaa 288Asp Val Tyr Ala Asn Cys Tyr Asn Arg Met Arg Arg Asn Ser Glu Glu
85 90 95ttc tac cgt cgc ttc ccg caa tta cgg gaa act ttc cga ggg ttg gtt 336Phe Tyr Arg Arg Phe Pro Gln Leu Arg Glu Thr Phe Arg Gly Leu Val
100 105 110aat cgt gct cgc gcc ggg gag att gtg ctt ccc acc ggc gaa gtt gtg 384Asn Arg Ala Arg Ala Gly Glu Ile Val Leu Pro Thr Gly Glu Val Val
115 120 125tca gaa acc agg ctg cga tcc ctt ggt cac ttg ttg ggt agc aat gac 432Ser Glu Thr Arg Leu Arg Ser Leu Gly His Leu Leu Gly Ser Asn Asp
130 135 140ggc tgg ttt gat ctg tac aac ctg ctg gaa tta gat ccc acc tcc aac 480Gly Trp Phe Asp Leu Tyr Asn Leu Leu Glu Leu Asp Pro Thr Ser Asn145 150 155 160gct ttt gtc cat gac ctg gca gga ctt ttg cct ttc ggc aac cgc aac 528Ala Phe Val His Asp Leu Ala Gly Leu Leu Pro Phe Gly Asn Arg Asn
165 170 175cca att tat tac gtg ctc cat gag tcc tct tac gcc gac ggt gtg gtg 576Pro Ile Tyr Tyr Val Leu His Glu Ser Ser Tyr Ala Asp Gly Val Val
180 185 190aca aat tgg gca gca gag cgt gtg ctt cca gag gat ttc cgc gag gat 624Thr Asn Trp Ala Ala Glu Arg Val Leu Pro Glu Asp Phe Arg Glu Asp
195 200 205cca aca ctg ctc acc ggt gag cac gtg ttc cag gag tgg aca gac acc 672Pro Thr Leu Leu Thr Gly Glu His Val Phe Gln Glu Trp Thr Asp Thr
210 215 220gtg ccg tcg ctc aag ccg tgg aag gac gtt gcc ctg gca ttg gct cag 720Val Pro Ser Leu Lys Pro Trp Lys Asp Val Ala Leu Ala Leu Ala Gln225 230 235 240cag gaa tgg ccc aag ctt tat gat gcg aag gca ttg gaa aac tca cag 768Gln Glu Trp Pro Lys Leu Tyr Asp Ala Lys Ala Leu Glu Asn Ser Gln
245 250 255gcc aag ggc gct gca gca gtg tat gcc aat gac gtt ttc gtc cca gtg 816Ala Lys Gly Ala Ala Ala Val Tyr Ala Asn Asp Val Phe Val Pro Val
260 265 270gat tac tct ctg gaa acc gca caa cac ctg ccc ggt gtg cag ctg ttt 864Asp Tyr Ser Leu Glu Thr Ala Gln His Leu Pro Gly Val Gln Leu Phe
275 280 285atc acc agc cag cat gaa cac aat gga ctt cgt gcc agc tca ggc gca 912Ile Thr Ser Gln His Glu His Asn Gly Leu Arg Ala Ser Ser Gly Ala
290 295 300gta ctg aag cac ctt ttc gat ctg gcc cac ggc cga gag gta cgc 957Val Leu Lys His Leu Phe Asp Leu Ala His Gly Arg Glu Val Arg305 310 315tgattcctcg tgttagtact agc 980<210>98<211>319<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>98Gly Asn Asp Ile Leu Glu Lys Pro Thr Ala Glu Val Val Glu Tyr Leu1 5 10 15Ser His Leu Arg Ala Asp Gly Ile Val Arg Asp Ala Glu Ala Leu Arg
20 25 30Lys His Leu Gly Val Asn Gln Trp Asn Leu Leu Gly Gln Ser Phe Gly
35 40 45Gly Phe Thr Thr Leu His Tyr Leu Ser Arg His Ala Asp Ser Leu Asp
50 55 60Asn Val Phe Ile Thr Gly Gly Leu Ser Ala Ile Asp Arg Pro Ala Glu65 70 75 80Asp Val Tyr Ala Asn Cys Tyr Asn Arg Met Arg Arg Asn Ser Glu Glu
85 90 95Phe Tyr Arg Arg Phe Pro Gln Leu Arg Glu Thr Phe Arg Gly Leu Val
100 105 110Asn Arg Ala Arg Ala Gly Glu Ile Val Leu Pro Thr Gly Glu Val Val
115 120 125Ser Glu Thr Arg Leu Arg Ser Leu Gly His Leu Leu Gly Ser Asn Asp
130 135 140Gly Trp Phe Asp Leu Tyr Asn Leu Leu Glu Leu Asp Pro Thr Ser Asn145 150 155 160Ala Phe Val His Asp Leu Ala Gly Leu Leu Pro Phe Gly Asn Arg Asn
165 170 175Pro Ile Tyr Tyr Val Leu His Glu Ser Ser Tyr Ala Asp Gly Val Val
180 185 190Thr Asn Trp Ala Ala Glu Arg Val Leu Pro Glu Asp Phe Arg Glu Asp
195 200 205Pro Thr Leu Leu Thr Gly Glu His Val Phe Gln Glu Trp Thr Asp Thr
210 215 220Val Pro Ser Leu Lys Pro Trp Lys Asp Val Ala Leu Ala Leu Ala Gln225 230 235 240Gln Glu Trp Pro Lys Leu Tyr Asp Ala Lys Ala Leu Glu Asn Ser Gln
245 250 255Ala Lys Gly Ala Ala Ala Val Tyr Ala Asn Asp Val Phe Val Pro Val
260 265 270Asp Tyr Ser Leu Glu Thr Ala Gln His Leu Pro Gly Val Gln Leu Phe
275 280 285Ile Thr Ser Gln His Glu His Asn Gly Leu Arg Ala Ser Ser Gly Ala
290 295 300Val Leu Lys His Leu Phe Asp Leu Ala His Gly Arg Glu Val Arg305 310 315<210>99<211>1788<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1765)<223>RXN00877<400>99aaataatggg gctcgccggt gatggttccc gccgggcatt caacggtgac ggaagaggtg 60gcagacatga tgaaaactct agcaactagt atcggtcact atg act gtt gaa cac 115
Met Thr Val Glu His
1 5ctg ctc aag ccc agc acc ttg ccc tac cag ctg ccc gat ttc gca gcg 163Leu Leu Lys Pro Ser Thr Leu Pro Tyr Gln Leu Pro Asp Phe Ala Ala
10 15 20atc aag gtg gct gat ttc ccg ccc gcc ttc gaa ctc gca tta gct gaa 211Ile Lys Val Ala Asp Phe Pro Pro Ala Phe Glu Leu Ala Leu Ala Glu
25 30 35cac gat gct gaa att aca gcg atc gct acc aat gag gac gct cct acc 259His Asp Ala Glu Ile Thr Ala Ile Ala Thr Asn Glu Asp Ala Pro Thr
40 45 50tgg gag aac acc att gag gcc ctg gaa cgc gca ggc ctg tcc ctc aac 307Trp Glu Asn Thr Ile Glu Ala Leu Glu Arg Ala Gly Leu Ser Leu Asn
55 60 65cgc gtc gcc gcc gta ttc ttc aac ttg cag ggc acc gat tcc tcc cct 355Arg Val Ala Ala Val Phe Phe Asn Leu Gln Gly Thr Asp Ser Ser Pro70 75 80 85gaa atg gat gaa atc gca gcc act atc gcg ccg aaa ctc tcc gcg cat 403Glu Met Asp Glu Ile Ala Ala Thr Ile Ala Pro Lys Leu Ser Ala His
90 95 100tcg gat gcg att ttc cac aat gct gcg ctt ttc gcg cgc att gag gcc 451Ser Asp Ala Ile Phe His Asn Ala Ala Leu Phe Ala Arg Ile Glu Ala
105 110 115gta gaa gca ccg gcc gac gag gaa tcg caa cgc ctg ttg tcc cac acc 499Val Glu Ala Pro Ala Asp Glu Glu Ser Gln Arg Leu Leu Ser His Thr
120 125 130aag cgc gct ttt cga cgt cgc ggt gca gca ctc aac gcc gac ggc aag 547Lys Arg Ala Phe Arg Arg Arg Gly Ala Ala Leu Asn Ala Asp Gly Lys
135 140 145gcc cga ctg agc acc atc aac cag cgc cta tcg gca ctg tcc gaa cag 595Ala Arg Leu Ser Thr Ile Asn Gln Arg Leu Ser Ala Leu Ser Glu Gln150 155 160 165ttc ggc cgc aac ctg ctt cag gac acc cgc gat ctg gcg gtc aac ttt 643Phe Gly Arg Asn Leu Leu Gln Asp Thr Arg Asp Leu Ala Val Asn Phe
170 175 180gaa gaa tct gaa ctt gcc ggt ttt agc gaa gcc cgc ata tcc gcc gcc 691Glu Glu Ser Glu Leu Ala Gly Phe Ser Glu Ala Arg Ile Ser Ala Ala
185 190 195gct gac tac gca gca gca gtt ggc acc gaa ggc tac gtg gtt cca ctg 739Ala Asp Tyr Ala Ala Ala Val Gly Thr Glu Gly Tyr Val Val Pro Leu
200 205 210gaa ctg ccc acc gtg cag tca gag cag gca gta tta acc gaa tcc gcc 787Glu Leu Pro Thr Val Gln Ser Glu Gln Ala Val Leu Thr Glu Ser Ala
215 220 225tcg cgt gca aag ctt tat gaa gcc tcc cag aag cgt ggc gcc agc ctg 835Ser Arg Ala Lys Leu Tyr Glu Ala Ser Gln Lys Arg Gly Ala Ser Leu230 235 240 245aac aag gac gtg ctg ctc gaa acc gtg cgt ctg cgt gct gaa cgc gcc 883Asn Lys Asp Val Leu Leu Glu Thr Val Arg Leu Arg Ala Glu Arg Ala
250 255 260aca ctt tta ggc tac gac acc cac gcc gat tac gtc atc gaa gaa gaa 931Thr Leu Leu Gly Tyr Asp Thr His Ala Asp Tyr Val Ile Glu Glu Glu
265 270 275acc gcc gat gac gtc gca gcc gtg cgc gcc ttg ctt tat gat ctc gcc 979Thr Ala Asp Asp Val Ala Ala Val Arg Ala Leu Leu Tyr Asp Leu Ala
280 285 290cca gcc gcc tct gcc aat gcg aaa gcc gaa tac aaa ctc tcc gca gaa 1027Pro Ala Ala Ser Ala Asn Ala Lys Ala Glu Tyr Lys Leu Ser Ala Glu
295 300 305gaa gca gaa gaa cac ggc caa aaa gtc ggc gca gct gac tgg agc ttc 1075Glu Ala Glu Glu His Gly Gln Lys Val Gly Ala Ala Asp Trp Ser Phe310 315 320 325tgg gaa gcc aaa gtc cgc gcc cgc gac tac gcc ctg gac gaa acc gaa 1123Trp Glu Ala Lys Val Arg Ala Arg Asp Tyr Ala Leu Asp Glu Thr Glu
330 335 340ctg cgc aac tac ttc cca ttg aac caa gta ctc cgt gac ggc gtc ttc 1171Leu Arg Asn Tyr Phe Pro Leu Asn Gln Val Leu Arg Asp Gly Val Phe
345 350 355ttc gct gct aac cgc ctc tac gga atc acc gtg gaa cca cgc cct gac 1219Phe Ala Ala Asn Arg Leu Tyr Gly Ile Thr Val Glu Pro Arg Pro Asp
360 365 370ctg cgc ggt tac gcc gag ggc gtg gac gtc tgg gaa gtc ctc gat tct 1267Leu Arg Gly Tyr Ala Glu Gly Val Asp Val Trp Glu Val Leu Asp Ser
375 380 385gac ggc tcc ggc atc ggc ctg atc ctt acc gac tac tac ggc cga cca 1315Asp Gly Ser Gly Ile Gly Leu Ile Leu Thr Asp Tyr Tyr Gly Arg Pro390 395 400 405tcc aag cgg ggc ggc gct tgg atg tcc agc ttt gtc gac caa tcc gag 1363Ser Lys Arg Gly Gly Ala Trp Met Ser Ser Phe Val Asp Gln Ser Glu
410 415 420ctg cta ggc acc aag cca gtc gtg gtc aac gtt atg ggt att acc aaa 1411Leu Leu Gly Thr Lys Pro Val Val Val Asn Val Met Gly Ile Thr Lys
425 430 435cca acc acc ggc gaa gca cta ctc agc ctc gat gaa gta acc acc atc 1459Pro Thr Thr Gly Glu Ala Leu Leu Ser Leu Asp Glu Val Thr Thr Ile
440 445 450ttc cac gaa ttc ggc cac ggc ctg cac ggc ttg ctg tcc aag gtg cgc 1507Phe His Glu Phe Gly His Gly Leu His Gly Leu Leu Ser Lys Val Arg
455 460 465tac cca agc ttc tcc gga acc tcc gtg ccc cgc gac tac gta gaa ttc 1555Tyr Pro Ser Phe Ser Gly Thr Ser Val Pro Arg Asp Tyr Val Glu Phe470 475 480 485ccc tcc cag atc aac gaa aac tgg gca ttc gac cct gca gta gtc cgc 1603Pro Ser Gln Ile Asn Glu Asn Trp Ala Phe Asp Pro Ala Val Val Arg
490 495 500aac tac gcc cgc cac gtg gac acc ggc gac atc att cca gac tcc ctg 1651Asn Tyr Ala Arg His Val Asp Thr Gly Asp Ile Ile Pro Asp Ser Leu
505 510 515ctt gag gca gtg gaa gca tgt ggc att tca gac aga gtg gtg gaa cat 1699Leu Glu Ala Val Glu Ala Cys Gly Ile Ser Asp Arg Val Val Glu His
520 525 530gtg agt act tgt ccc cat cta tta tcg acc tgc cct gtc tct ccc tgt 1747Val Ser Thr Cys Pro His Leu Leu Ser Thr Cys Pro Val Ser Pro Cys
535 540 545cca cag cgg atg ccg cac tagtcaatga cattgaccaa tta 1788Pro Gln Arg Met Pro His550 555<210>100<211>555<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>100Met Thr Val Glu His Leu Leu Lys Pro Ser Thr Leu Pro Tyr Gln Leu1 5 10 15Pro Asp Phe Ala Ala Ile Lys Val Ala Asp Phe Pro Pro Ala Phe Glu
20 25 30Leu Ala Leu Ala Glu His Asp Ala Glu Ile Thr Ala Ile Ala Thr Asn
35 40 45Glu Asp Ala Pro Thr Trp Glu Asn Thr Ile Glu Ala Leu Glu Arg Ala
50 55 60Gly Leu Ser Leu Asn Arg Val Ala Ala Val Phe Phe Asn Leu Gln Gly65 70 75 80Thr Asp Ser Ser Pro Glu Met Asp Glu Ile Ala Ala Thr Ile Ala Pro
85 90 95Lys Leu Ser Ala His Ser Asp Ala Ile Phe His Asn Ala Ala Leu Phe
100 105 110Ala Arg Ile Glu Ala Val Glu Ala Pro Ala Asp Glu Glu Ser Gln Arg
115 120 125Leu Leu Ser His Thr Lys Arg Ala Phe Arg Arg Arg Gly Ala Ala Leu
130 135 140Asn Ala Asp Gly Lys Ala Arg Leu Ser Thr Ile Asn Gln Arg Leu Ser145 150 155 160Ala Leu Ser Glu Gln Phe Gly Arg Asn Leu Leu Gln Asp Thr Arg Asp
165 170 175Leu Ala Val Asn Phe Glu Glu Ser Glu Leu Ala Gly Phe Ser Glu Ala
180 185 190Arg Ile Ser Ala Ala Ala Asp Tyr Ala Ala Ala Val Gly Thr Glu Gly
195 200 205Tyr Val Val Pro Leu Glu Leu Pro Thr Val Gln Ser Glu Gln Ala Val
210 215 220Leu Thr Glu Ser Ala Ser Arg Ala Lys Leu Tyr Glu Ala Ser Gln Lys225 230 235 240Arg Gly Ala Ser Leu Asn Lys Asp Val Leu Leu Glu Thr Val Arg Leu
245 250 255Arg Ala Glu Arg Ala Thr Leu Leu Gly Tyr Asp Thr His Ala Asp Tyr
260 265 270Val Ile Glu Glu Glu Thr Ala Asp Asp Val Ala Ala Val Arg Ala Leu
275 280 285Leu Tyr Asp Leu Ala Pro Ala Ala Ser Ala Asn Ala Lys Ala Glu Tyr
290 295 300Lys Leu Ser Ala Glu Glu Ala Glu Glu His Gly Gln Lys Val Gly Ala305 310 315 320Ala Asp Trp Ser Phe Trp Glu Ala Lys Val Arg Ala Arg Asp Tyr Ala
325 330 335Leu Asp Glu Thr Glu Leu Arg Asn Tyr Phe Pro Leu Asn Gln Val Leu
340 345 350Arg Asp Gly Val Phe Phe Ala Ala Asn Arg Leu Tyr Gly Ile Thr Val
355 360 365Glu Pro Arg Pro Asp Leu Arg Gly Tyr Ala Glu Gly Val Asp Val Trp
370 375 380Glu Val Leu Asp Ser Asp Gly Ser Gly Ile Gly Leu Ile Leu Thr Asp385 390 395 400Tyr Tyr Gly Arg Pro Ser Lys Arg Gly Gly Ala Trp Met Ser Ser Phe
405 410 415Val Asp Gln Ser Glu Leu Leu Gly Thr Lys Pro Val Val Val Asn Val
420 425 430Met Gly Ile Thr Lys Pro Thr Thr Gly Glu Ala Leu Leu Ser Leu Asp
435 440 445Glu Val Thr Thr Ile Phe His Glu Phe Gly His Gly Leu His Gly Leu
450 455 460Leu Ser Lys Val Arg Tyr Pro Ser Phe Ser Gly Thr Ser Val Pro Arg465 470 475 480Asp Tyr Val Glu Phe Pro Ser Gln Ile Asn Glu Asn Trp Ala Phe Asp
485 490 495Pro Ala Val Val Arg Asn Tyr Ala Arg His Val Asp Thr Gly Asp Ile
500 505 510Ile Pro Asp Ser Leu Leu Glu Ala Val Glu Ala Cys Gly Ile Ser Asp
515 520 525Arg Val Val Glu His Val Ser Thr Cys Pro His Leu Leu Ser Thr Cys
530 535 540Pro Val Ser Pro Cys Pro Gln Arg Met Pro His545 550 555<210>101<211>1088<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(1065)<223>FRXA00877<400>101gca gca gca gtt ggc acc gaa ggc tac gtg gtt cca ctg gaa ctg ccc 48Ala Ala Ala Val Gly Thr Glu Gly Tyr Val Val Pro Leu Glu Leu Pro1 5 10 15acc gtg cag tca gag cag gca gta tta acc gaa tcc gcc tcg cgt gca 96Thr Val Gln Ser Glu Gln Ala Val Leu Thr Glu Ser Ala Ser Arg Ala
20 25 30aag ctt tat gaa gcc tcc cag aag cgt ggc gcc agc ctg aac aag gac 144Lys Leu Tyr Glu Ala Ser Gln Lys Arg Gly Ala Ser Leu Asn Lys Asp
35 40 45gtg ctg ctc gaa acc gtg cgt ctg cgt gct gaa cgc gcc aca ctt tta 192Val Leu Leu Glu Thr Val Arg Leu Arg Ala Glu Arg Ala Thr Leu Leu
50 55 60ggc tac gac acc cac gcc gat tac gtc atc gaa gaa gaa acc gcc gat 240Gly Tyr Asp Thr His Ala Asp Tyr Val Ile Glu Glu Glu Thr Ala Asp65 70 75 80gac gtc gca gcc gtg cgc gcc ttg ctt tat gat ctc gcc cca gcc gcc 288Asp Val Ala Ala Val Arg Ala Leu Leu Tyr Asp Leu Ala Pro Ala Ala
85 90 95tct gcc aat gcg aaa gcc gaa tac aaa ctc tcc gca gaa gaa gca gaa 336Ser Ala Asn Ala Lys Ala Glu Tyr Lys Leu Ser Ala Glu Glu Ala Glu
100 105 110gaa cac ggc caa aaa gtc ggc gca gct gac tgg agc ttc tgg gaa gcc 384Glu His Gly Gln Lys Val Gly Ala Ala Asp Trp Ser Phe Trp Glu Ala
115 120 125aaa gtc cgc gcc cgc gac tac gcc ctg gac gaa acc gaa ctg cgc aac 432Lys Val Arg Ala Arg Asp Tyr Ala Leu Asp Glu Thr Glu Leu Arg Asn
130 135 140tac ttc cca ttg aac caa gta ctc cgt gac ggc gtc ttc ttc gct gct 480Tyr Phe Pro Leu Asn Gln Val Leu Arg Asp Gly Val Phe Phe Ala Ala145 150 155 160aac cgc ctc tac gga atc acc gtg gaa cca cgc cct gac ctg cgc ggt 528Asn Arg Leu Tyr Gly Ile Thr Val Glu Pro Arg Pro Asp Leu Arg Gly
165 170 175tac gcc gag ggc gtg gac gtc tgg gaa gtc ctc gat tct gac ggc tcc 576Tyr Ala Glu Gly Val Asp Val Trp Glu Val Leu Asp Ser Asp Gly Ser
180 185 190ggc atc ggc ctg atc ctt acc gac tac tac ggc cga cca tcc aag cgg 624Gly Ile Gly Leu Ile Leu Thr Asp Tyr Tyr Gly Arg Pro Ser Lys Arg
195 200 205ggc ggc gct tgg atg tcc agc ttt gtc gac caa tcc gag ctg cta ggc 672Gly Gly Ala Trp Met Ser Ser Phe Val Asp Gln Ser Glu Leu Leu Gly
210 215 220acc aag cca gtc gtg gtc aac gtt atg ggt att acc aaa cca acc acc 720Thr Lys Pro Val Val Val Asn Val Met Gly Ile Thr Lys Pro Thr Thr225 230 235 240ggc gaa gca cta ctc agc ctc gat gaa gta acc acc atc ttc cac gaa 768Gly Glu Ala Leu Leu Ser Leu Asp Glu Val Thr Thr Ile Phe His Glu
245 250 255ttc ggc cac ggc ctg cac ggc ttg ctg tcc aag gtg cgc tac cca agc 816Phe Gly His Gly Leu His Gly Leu Leu Ser Lys Val Arg Tyr Pro Ser
260 265 270ttc tcc gga acc tcc gtg ccc cgc gac tac gta gaa ttc ccc tcc cag 864Phe Ser Gly Thr Ser Val Pro Arg Asp Tyr Val Glu Phe Pro Ser Gln
275 280 285atc aac gaa aac tgg gca ttc gac cct gca gta gtc cgc aac tac gcc 912Ile Asn Glu Asn Trp Ala Phe Asp Pro Ala Val Val Arg Asn Tyr Ala
290 295 300cgc cac gtg gac acc ggc gac atc att cca gac tcc ctg ctt gag gca 960Arg His Val Asp Thr Gly Asp Ile Ile Pro Asp Ser Leu Leu Glu Ala305 310 315 320gtg gaa gca tgt ggc att tca gac aga gtg gtg gaa cat gtg agt act 1008Val Glu Ala Cys Gly Ile Ser Asp Arg Val Val Glu His Val Ser Thr
325 330 335tgt ccc cat cta tta tcg acc tgc cct gtc tct ccc tgt cca cag cgg 1056Cys Pro His Leu Leu Ser Thr Cys Pro Val Ser Pro Cys Pro Gln Arg
340 345 350atg ccg cac tagtcaatga cattgaccaa tta 1088Met Pro His
355<210>102<211>355<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>102Ala Ala Ala Val Gly Thr Glu Gly Tyr Val Val Pro Leu Glu Leu Pro1 5 10 15Thr Val Gln Ser Glu Gln Ala Val Leu Thr Glu Ser Ala Ser Arg Ala
20 25 30Lys Leu Tyr Glu Ala Ser Gln Lys Arg Gly Ala Ser Leu Asn Lys Asp
35 40 45Val Leu Leu Glu Thr Val Arg Leu Arg Ala Glu Arg Ala Thr Leu Leu
50 55 60Gly Tyr Asp Thr His Ala Asp Tyr Val Ile Glu Glu Glu Thr Ala Asp65 70 75 80Asp Val Ala Ala Val Arg Ala Leu Leu Tyr Asp Leu Ala Pro Ala Ala
85 90 95Ser Ala Asn Ala Lys Ala Glu Tyr Lys Leu Ser Ala Glu Glu Ala Glu
100 105 110Glu His Gly Gln Lys Val Gly Ala Ala Asp Trp Ser Phe Trp Glu Ala
115 120 125Lys Val Arg Ala Arg Asp Tyr Ala Leu Asp Glu Thr Glu Leu Arg Asn
130 135 140Tyr Phe Pro Leu Asn Gln Val Leu Arg Asp Gly Val Phe Phe Ala Ala145 150 155 160Asn Arg Leu Tyr Gly Ile Thr Val Glu Pro Arg Pro Asp Leu Arg Gly
165 170 175Tyr Ala Glu Gly Val Asp Val Trp Glu Val Leu Asp Ser Asp Gly Ser
180 185 190Gly Ile Gly Leu Ile Leu Thr Asp Tyr Tyr Gly Arg Pro Ser Lys Arg
195 200 205Gly Gly Ala Trp Met Ser Ser Phe Val Asp Gln Ser Glu Leu Leu Gly
210 215 220Thr Lys Pro Val Val Val Asn Val Met Gly Ile Thr Lys Pro Thr Thr225 230 235 240Gly Glu Ala Leu Leu Ser Leu Asp Glu Val Thr Thr Ile Phe His Glu
245 250 255Phe Gly His Gly Leu His Gly Leu Leu Ser Lys Val Arg Tyr Pro Ser
260 265 270Phe Ser Gly Thr Ser Val Pro Arg Asp Tyr Val Glu Phe Pro Ser Gln
275 280 285Ile Asn Glu Asn Trp Ala Phe Asp Pro Ala Val Val Arg Asn Tyr Ala
290 295 300Arg His Val Asp Thr Gly Asp Ile Ile Pro Asp Ser Leu Leu Glu Ala305 310 315 320Val Glu Ala Cys Gly Ile Ser Asp Arg Val Val Glu His Val Ser Thr
325 330 335Cys Pro His Leu Leu Ser Thr Cys Pro Val Ser Pro Cys Pro Gln Arg
340 345 350Met Pro His
355<210>103<211>2724<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(2701)<223>RXN01014<400>103tcttaaagtt ttctagcaat ccacactagg cgcgaactat cgtggtgtca ttgcgcacct 60tctaagggta gcgccccctc aaatttcaag gagcattaaa ttg acg tcc act aat 115
Leu Thr Ser Thr Asn
1 5ctc acc cga cag gaa gct tcg gat cgt tcg agg tta ctg agt gta gaa 163Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg Leu Leu Ser Val Glu
10 15 20aac tat gac att gca ctt gat ctc aac aac ggt gat gag ttt ttt agt 211Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly Asp Glu Phe Phe Ser
25 30 35tcc tcc acc gtt gtc agc ttc act gtc agg aag gct ggc gat acc ttt 259Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys Ala Gly Asp Thr Phe
40 45 50att gat ctc cgc gca gca agc gtt gag gag gtt cgc ctg gac aat gtg 307Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val Arg Leu Asp Asn Val
55 60 65tcc atc aaa gat gag gct cta acc ctt ggc aag aac ggc tac gac gag 355Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys Asn Gly Tyr Asp Glu70 75 80 85acg ttc ggc atc gcc ctg aag ggt ctt act ccc ggc gcg cac acc ttg 403Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro Gly Ala His Thr Leu
90 95 100cgg gta acg gcg tct atc ccc tat tcc cgc acc ggt gaa ggc ctg cac 451Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr Gly Glu Gly Leu His
105 110 115cgc atg gtg gat cca gca gac aat gag gtg tat ttg tac acc cag ttt 499Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr Leu Tyr Thr Gln Phe
120 125 130gag acc gcc gat gcc aag cgt atg ttc gcg tgt ttc gat cag cca gac 547Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys Phe Asp Gln Pro Asp
135 140 145ctc aag gct acc tat gat ctg aac atc aaa act cct aag ggt tgg aag 595Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr Pro Lys Gly Trp Lys150 155 160 165atc att tcc aac tct gag cag cag gtt tcc act cag cac act gat tac 643Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr Gln His Thr Asp Tyr
170 175 180gat acc cac att tcc cga gtg gac tat ccc ctc tcc acc tac ctg att 691Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu Ser Thr Tyr Leu Ile
185 190 195gcg gtg tgc gcg ggt cgt tac cac gag gtg tgc gat gtc tgg aag ggt 739Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys Asp Val Trp Lys Gly
200 205 210acg ctc acc cac cat gca gaa aca cct gcc gat cag cca act gag ctg 787Thr Leu Thr His His Ala Glu Thr Pro Ala Asp Gln Pro Thr Glu Leu
215 220 225act gtt ccg ctt gct ctc tac tgc cgc agt tct ttg gct aaa gat ctt 835Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser Leu Ala Lys Asp Leu230 235 240 245gat gcg gtg cgt ctg ttt acc gaa acg aag cag ggc ttt gat tgg tac 883Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln Gly Phe Asp Trp Tyr
250 255 260cac cgc aac ttc ggt gtg gcg tac cca ttc ggc aag tac gat cag atc 931His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly Lys Tyr Asp Gln Ile
265 270 275ttc gtc cct gaa ttt aat gct ggc gcg atg gag aac gcc ggc gct gtc 979Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu Asn Ala Gly Ala Val
280 285 290acc atc cgc gat gag tac gtt ttt gca tcc aag gca acc cgt tac cgc 1027Thr Ile Arg Asp Glu Tyr Val Phe Ala Ser Lys Ala Thr Arg Tyr Arg
295 300 305tac gag cgc cgc gct gaa acc atc ctt cac gag ctc gct cac atg tgg 1075Tyr Glu Arg Arg Ala Glu Thr Ile Leu His Glu Leu Ala His Met Trp310 315 320 325ttc ggt gtg ctg gtg acc atg cag tgg tgg gat gat ctg tgg ctc aac 1123Phe Gly Val Leu Val Thr Met Gln Trp Trp Asp Asp Leu Trp Leu Asn
330 335 340gag tcc ttc gcc act tgg tcc gcg gca att tct cag gct gag gaa act 1171Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile Ser Gln Ala Glu Glu Thr
345 350 355gaa tac aac act gca tgg gtg act ttc gcc aat gtg gag aag tcg tgg 1219Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala Asn Val Glu Lys Ser Trp
360 365 370gcg tac cag cag gat cag ctg cct tcc acc cac ccg gtg ttc tct gac 1267Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr His Pro Val Phe Ser Asp
375 380 385gga tac gac att gag act gtc gac cag aac ttc gac ggc atc acc tac 1315Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn Phe Asp Gly Ile Thr Tyr390 395 400 405gca aag ggc gcc tcg gtg ctc aag cag ctg cag gca tac gtt ggc cgt 1363Ala Lys Gly Ala Ser Val Leu Lys Gln Leu Gln Ala Tyr Val Gly Arg
410 415 420gag gaa ttc ctg gca ggc gta cgc agg cac ttt gcc aac cac gca tgg 1411Glu Glu Phe Leu Ala Gly Val Arg Arg His Phe Ala Asn His Ala Trp
425 430 435ggc aac gcc agc ttt gat gat ctg ctc ggc gcc ctc gag cag tcc tcc 1459Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly Ala Leu Glu Gln Ser Ser
440 445 450ggc cgc gac ctc tcc gac tgg gca aac cag tgg ctc aag acc acc ggc 1507Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln Trp Leu Lys Thr Thr Gly
455 460 465atc aac acc ctc ggc gca aag ttc acc acc gac aac ggc aaa tac acc 1555Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr Asp Asn Gly Lys Tyr Thr470 475 480 485tcc ttc tcc gtc acc cag acc ggc gcc gcg ccg ggt gcc ggt gag ctg 1603Ser Phe Ser Val Thr Gln Thr Gly Ala Ala Pro Gly Ala Gly Glu Leu
490 495 500cgg act cac cgc atc gcg gtg ggt ctt tat aag ctt gtc gac gga tcc 1651Arg Thr His Arg Ile Ala Val Gly Leu Tyr Lys Leu Val Asp Gly Ser
505 510 515ctc aac cgc tac gca cga gta gaa ctt gac tgc agt ggc gcg tcg aca 1699Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp Cys Ser Gly Ala Ser Thr
520 525 530agc gtt gaa gag atc gtt gga ctt gag cag gct gac ttc gtg ctg gtc 1747Ser Val Glu Glu Ile Val Gly Leu Glu Gln Ala Asp Phe Val Leu Val
535 540 545aac gat gat gat ctg acg tat gcg ctg ctg gat ctg gat gat gat tca 1795Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu Asp Leu Asp Asp Asp Ser550 555 560 565cgc aat ttt gtc atc gac aat att gat aag ttc agc gac cct atg cct 1843Arg Asn Phe Val Ile Asp Asn Ile Asp Lys Phe Ser Asp Pro Met Pro
570 575 580cgc acg ctg gtg tgg tcc gct gcg tgg gag atg act cgc gct ggt cag 1891Arg Thr Leu Val Trp Ser Ala Ala Trp Glu Met Thr Arg Ala Gly Gln
585 590 595atg aag gct cgt gat ttc atc gcg ctg gtt gct cgt ggc gct gct gcg 1939Met Lys Ala Arg Asp Phe Ile Ala Leu Val Ala Arg Gly Ala Ala Ala
600 605 610gaa act gaa att gct gtg ctg gag cgc att ctc gcg cag gct acc tct 1987Glu Thr Glu Ile Ala Val Leu Glu Arg Ile Leu Ala Gln Ala Thr Ser
615 620 625gcg ctg aag agc tac gcc gac cca gcg tgg gca gaa gca act gga aat 2035Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp Ala Glu Ala Thr Gly Asn630 635 640 645gac ctg ctg gcc gat gct ttc ctt gag ggt gct cgc tcc gca gaa cca 2083Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly Ala Arg Ser Ala Glu Pro
650 655 660gac tcc gac act cag ttg gcg ttc att cag gct ctg gca aaa gca acg 2131Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln Ala Leu Ala Lys Ala Thr
665 670 675ctc aat gat gct gct gcc gat tac ttc cgc gac att ctt gcc ggc aac 2179Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg Asp Ile Leu Ala Gly Asn
680 685 690gtc gaa ggc ctg acc gtg gat cct gac ctg cgt tgg tgg gca ctg act 2227Val Glu Gly Leu Thr Val Asp Pro Asp Leu Arg Trp Trp Ala Leu Thr
695 700 705gcg ctt atc gcc cgt ggt gac atc gag gct gtc gaa gat gca atc gcc 2275Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala Val Glu Asp Ala Ile Ala710 715 720 725gct gaa ctt tcc cgc gac aac tcc agt gcc tcc ttc ctc gca tca ctt 2323Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala Ser Phe Leu Ala Ser Leu
730 735 740cga gcc ggt gcc gct gtg aac act gaa gaa gtg aag gct gct gca tac 2371Arg Ala Gly Ala Ala Val Asn Thr Glu Glu Val Lys Ala Ala Ala Tyr
745 750 755aag cat gtc acg gca gtt gat agt ggc cta tcc aac ctg gag ctg cgc 2419Lys His Val Thr Ala Val Asp Ser Gly Leu Ser Asn Leu Glu Leu Arg
760 765 770cac aag att gaa ggc ctc aca ttc act ggc tct tct gaa ctg ctg caa 2467His Lys Ile Glu Gly Leu Thr Phe Thr Gly Ser Ser Glu Leu Leu Gln
775 780 785gcc tac aac gag cag tac ttc gaa atc ctt gat gat gtg tgg gcg aac 2515Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu Asp Asp Val Trp Ala Asn790 795 800 805ttc tcc ggc gaa atg gca cag cag atc gtc ctc gga ctg ttc cct tca 2563Phe Ser Gly Glu Met Ala Gln Gln Ile Val Leu Gly Leu Phe Pro Ser
810 815 820tgg aac gtt tcc gaa gag ggt ctc aag cgt acc gac gag ttt ctt gat 2611Trp Asn Val Ser Glu Glu Gly Leu Lys Arg Thr Asp Glu Phe Leu Asp
825 830 835ggc gaa cat gtc gca ggc atc aag cga att gtt tcc gaa tcc ctc gac 2659Gly Glu His Val Ala Gly Ile Lys Arg Ile Val Ser Glu Ser Leu Asp
840 845 850cgc act gcc cgt gct ctg cgc aac cgt gcg gca gat gct gcg 2701Arg Thr Ala Arg Ala Leu Arg Asn Arg Ala Ala Asp Ala Ala
855 860 865taagtaaaag attctcaatc cca 2724<210>104<211>867<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>104Leu Thr Ser Thr Asn Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg1 5 10 15Leu Leu Ser Val Glu Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly
20 25 30Asp Glu Phe Phe Ser Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys
35 40 45Ala Gly Asp Thr Phe Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val
50 55 60Arg Leu Asp Asn Val Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys65 70 75 80Asn Gly Tyr Asp Glu Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro
85 90 95Gly Ala His Thr Leu Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr
100 105 110Gly Glu Gly Leu His Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr
115 120 125Leu Tyr Thr Gln Phe Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys
130 135 140Phe Asp Gln Pro Asp Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr145 150 155 160Pro Lys Gly Trp Lys Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr
165 170 175Gln His Thr Asp Tyr Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu
180 185 190Ser Thr Tyr Leu Ile Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys
195 200 205Asp Val Trp Lys Gly Thr Leu Thr His His Ala Glu Thr Pro Ala Asp
210 215 220Gln Pro Thr Glu Leu Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser225 230 235 240Leu Ala Lys Asp Leu Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln
245 250 255Gly Phe Asp Trp Tyr His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly
260 265 270Lys Tyr Asp Gln Ile Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu
275 280 285Asn Ala Gly Ala Val Thr Ile Arg Asp Glu Tyr Val Phe Ala Ser Lys
290 295 300Ala Thr Arg Tyr Arg Tyr Glu Arg Arg Ala Glu Thr Ile Leu His Glu305 310 315 320Leu Ala His Met Trp Phe Gly Val Leu Val Thr Met Gln Trp Trp Asp
325 330 335Asp Leu Trp Leu Asn Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile Ser
340 345 350Gln Ala Glu Glu Thr Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala Asn
355 360 365Val Glu Lys Ser Trp Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr His
370 375 380Pro Val Phe Ser Asp Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn Phe385 390 395 400Asp Gly lle Thr Tyr Ala Lys Gly Ala Ser Val Leu Lys Gln Leu Gln
405 410 415Ala Tyr Val Gly Arg Glu Glu Phe Leu Ala Gly Val Arg Arg His Phe
420 425 430Ala Asn His Ala Trp Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly Ala
435 440 445Leu Glu Gln Ser Ser Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln Trp
450 455 460Leu Lys Thr Thr Gly Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr Asp465 470 475 480Asn Gly Lys Tyr Thr Ser Phe Ser Val Thr Gln Thr Gly Ala Ala Pro
485 490 495Gly Ala Gly Glu Leu Arg Thr His Arg Ile Ala Val Gly Leu Tyr Lys
500 505 510Leu Val Asp Gly Ser Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp Cys
515 520 525Ser Gly Ala Ser Thr Ser Val Glu Glu Ile Val Gly Leu Glu Gln Ala
530 535 540Asp Phe Val Leu Val Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu Asp545 550 555 560Leu Asp Asp Asp Ser Arg Asn Phe Val Ile Asp Asn Ile Asp Lys Phe
565 570 575Ser Asp Pro Met Pro Arg Thr Leu Val Trp Ser Ala Ala Trp Glu Met
580 585 590Thr Arg Ala Gly Gln Met Lys Ala Arg Asp Phe Ile Ala Leu Val Ala
595 600 605Arg Gly Ala Ala Ala Glu Thr Glu Ile Ala Val Leu Glu Arg Ile Leu
610 615 620Ala Gln Ala Thr Ser Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp Ala625 630 635 640Glu Ala Thr Gly Asn Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly Ala
645 650 655Arg Ser Ala Glu Pro Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln Ala
660 665 670Leu Ala Lys Ala Thr Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg Asp
675 680 685Ile Leu Ala Gly Asn Val Glu Gly Leu Thr Val Asp Pro Asp Leu Arg
690 695 700Trp Trp Ala Leu Thr Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala Val705 710 715 720Glu Asp Ala Ile Ala Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala Ser
725 730 735Phe Leu Ala Ser Leu Arg Ala Gly Ala Ala Val Asn Thr Glu Glu Val
740 745 750Lys Ala Ala Ala Tyr Lys His Val Thr Ala Val Asp Ser Gly Leu Ser
755 760 765Asn Leu Glu Leu Arg His Lys Ile Glu Gly Leu Thr Phe Thr Gly Ser
770 775 780Ser Glu Leu Leu Gln Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu Asp785 790 795 800Asp Val Trp Ala Asn Phe Ser Gly Glu Met Ala Gln Gln Ile Val Leu
805 810 815Gly Leu Phe Pro Ser Trp Asn Val Ser Glu Glu Gly Leu Lys Arg Thr
820 825 830Asp Glu Phe Leu Asp Gly Glu His Val Ala Gly Ile Lys Arg Ile Val
835 840 845Ser Glu Ser Leu Asp Arg Thr Ala Arg Ala Leu Arg Asn Arg Ala Ala
850 855 860Asp Ala Ala865<210>105<211>1578<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(1578)<223>FRXA01014<400>105gat gat ctg tgg ctc aac gag tcc ttc gcc act tgg tcc gcg gca att 48Asp Asp Leu Trp Leu Asn Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile1 5 10 15tct cag gct gag gaa act gaa tac aac act gca tgg gtg act ttc gcc 96Ser Gln Ala Glu Glu Thr Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala
20 25 30aat gtg gag aag tcg tgg gcg tac cag cag gat cag ctg cct tcc acc 144Asn Val Glu Lys Ser Trp Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr
35 40 45cac ccg gtg ttc tct gac gga tac gac att gag act gtc gac cag aac 192His Pro Val Phe Ser Asp Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn
50 55 60ttc gac ggc atc acc tac gca aag ggc gcc tcg gtg ctc aag cag ctg 240Phe Asp Gly Ile Thr Tyr Ala Lys Gly Ala Ser Val Leu Lys Gln Leu65 70 75 80cag gca tac gtt ggc cgt gag gaa ttc ctg gca ggc gta cgc agg cac 288Gln Ala Tyr Val Gly Arg Glu Glu Phe Leu Ala Gly Val Arg Arg His
85 90 95ttt gcc aac cac gca tgg ggc aac gcc agc ttt gat gat ctg ctc ggc 336Phe Ala Asn His Ala Trp Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly
100 105 110gcc ctc gag cag tcc tcc ggc cgc gac ctc tcc gac tgg gca aac cag 384Ala Leu Glu Gln Ser Ser Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln
115 120 125tgg ctc aag acc acc ggc atc aac acc ctc ggc gca aag ttc acc acc 432Trp Leu Lys Thr Thr Gly Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr
130 135 140gac aac ggc aaa tac acc tcc ttc tcc gtc acc cag acc ggc gcc gcg 480Asp Asn Gly Lys Tyr Thr Ser Phe Ser Val Thr Gln Thr Gly Ala Ala145 150 155 160ccg ggt gcc ggt gag ctg cgg act cac cgc atc gcg gtg ggt ctt tat 528Pro Gly Ala Gly Glu Leu Arg Thr His Arg Ile Ala Val Gly Leu Tyr
165 170 175aag ctt gtc gac gga tcc ctc aac cgc tac gca cga gta gaa ctt gac 576Lys Leu Val Asp Gly Ser Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp
180 185 190tgc agt ggc gcg tcg aca agc gtt gaa gag atc gtt gga ctt gag cag 624Cys Ser Gly Ala Ser Thr Ser Val Glu Glu Ile Val Gly Leu Glu Gln
195 200 205gct gac ttc gtg ctg gtc aac gat gat gat ctg acg tat gcg ctg ctg 672Ala Asp Phe Val Leu Val Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu
210 215 220gat ctg gat gat gat tca cgc aat ttt gtc atc gac aat att gat aag 720Asp Leu Asp Asp Asp Ser Arg Asn Phe Val Ile Asp Asn Ile Asp Lys225 230 235 240ttc agc gac cct atg cct cgc acg ctg gtg tgg tcc gct gcg tgg gag 768Phe Ser Asp Pro Met Pro Arg Thr Leu Val Trp Ser Ala Ala Trp Glu
245 250 255atg act cgc gct ggt cag atg aag gct cgt gat ttc atc gcg ctg gtt 816Met Thr Arg Ala Gly Gln Met Lys Ala Arg Asp Phe Ile Ala Leu Val
260 265 270gct cgt ggc gct gct gcg gaa act gaa att gct gtg ctg gag cgc att 864Ala Arg Gly Ala Ala Ala Glu Thr Glu Ile Ala Val Leu Glu Arg Ile
275 280 285ctc gcg cag gct acc tct gcg ctg aag agc tac gcc gac cca gcg tgg 912Leu Ala Gln Ala Thr Ser Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp
290 295 300gca gaa gca act gga aat gac ctg ctg gcc gat gct ttc ctt gag ggt 960Ala Glu Ala Thr Gly Asn Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly305 310 315 320gct cgc tcc gca gaa cca gac tcc gac act cag ttg gcg ttc att cag 1008Ala Arg Ser Ala Glu Pro Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln
325 330 335gct ctg gca aaa gca acg ctc aat gat gct gct gcc gat tac ttc cgc 1056Ala Leu Ala Lys Ala Thr Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg
340 345 350gac att ctt gcc ggc aac gtc gaa ggc ctg acc gtg gat cct gac ctg 1104Asp Ile Leu Ala Gly Asn Val Glu Gly Leu Thr Val Asp Pro Asp Leu
355 360 365cgt tgg tgg gca ctg act gcg ctt atc gcc cgt ggt gac atc gag gct 1152Arg Trp Trp Ala Leu Thr Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala
370 375 380gtc gaa gat gca atc gcc gct gaa ctt tcc cgc gac aac tcc agt gcc 1200Val Glu Asp Ala Ile Ala Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala385 390 395 400tcc ttc ctc gca tca ctt cga gcc ggt gcc gct gtg aac act gaa gaa 1248Ser Phe Leu Ala Ser Leu Arg Ala Gly Ala Ala Val Asn Thr Glu Glu
405 410 415gtg aag gct gct gca tac aag cat gtc ccg gca gtt gat agt ggc cta 1296Val Lys Ala Ala Ala Tyr Lys His Val Pro Ala Val Asp Ser Gly Leu
420 425 430tcc aac ctg gag ctg cgc cac aag att gaa ggc ctc aca ttc act ggc 1344Ser Asn Leu Glu Leu Arg His Lys Ile Glu Gly Leu Thr Phe Thr Gly
435 440 445tct ttt gaa ctg ctg caa gcc tac aac gag cag tac ttc gaa atc ctt 1392Ser Phe Glu Leu Leu Gln Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu
450 455 460gat gat gtg tgg gcg aac ttc tcc ggc gaa atg gca cag cag atc gtc 1440Asp Asp Val Trp Ala Asn Phe Ser Gly Glu Met Ala Gln Gln Ile Val465 470 475 480ctc gga ctg ttc cct tca tgg aac gtt tcc gaa gag ggt ctc aag cgt 1488Leu Gly Leu Phe Pro Ser Trp Asn Val Ser Glu Glu Gly Leu Lys Arg
485 490 495acc gac gag ttt ctt gat ggc gaa cat gtc gca ggc atc aag cga att 1536Thr Asp Glu Phe Leu Asp Gly Glu His Val Ala Gly Ile Lys Arg Ile
500 505 510gtt tcc gaa tcc ctc gac cgc act gcc cgt gct ctg cgc aac 1578Val Ser Glu Ser Leu Asp Arg Thr Ala Arg Ala Leu Arg Asn
515 520 525<210>106<211>526<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>106Asp Asp Leu Trp Leu Asn Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile1 5 10 15Ser Gln Ala Glu Glu Thr Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala
20 25 30Asn Val Glu Lys Ser Trp Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr
35 40 45His Pro Val Phe Ser Asp Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn
50 55 60Phe Asp Gly Ile Thr Tyr Ala Lys Gly Ala Ser Val Leu Lys Gln Leu65 70 75 80Gln Ala Tyr Val Gly Arg Glu Glu Phe Leu Ala Gly Val Arg Arg His
85 90 95Phe Ala Asn His Ala Trp Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly
100 105 110Ala Leu Glu Gln Ser Ser Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln
115 120 125Trp Leu Lys Thr Thr Gly Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr
130 135 140Asp Asn Gly Lys Tyr Thr Ser Phe Ser Val Thr Gln Thr Gly Ala Ala145 150 155 160Pro Gly Ala Gly Glu Leu Arg Thr His Arg Ile Ala Val Gly Leu Tyr
165 170 175Lys Leu Val Asp Gly Ser Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp
180 185 190Cys Ser Gly Ala Ser Thr Ser Val Glu Glu Ile Val Gly Leu Glu Gln
195 200 205Ala Asp Phe Val Leu Val Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu
210 215 220Asp Leu Asp Asp Asp Ser Arg Asn Phe Val Ile Asp Asn Ile Asp Lys225 230 235 240Phe Ser Asp Pro Met Pro Arg Thr Leu Val Trp Ser Ala Ala Trp Glu
245 250 255Met Thr Arg Ala Gly Gln Met Lys Ala Arg Asp Phe Ile Ala Leu Val
260 265 270Ala Arg Gly Ala Ala Ala Glu Thr Glu Ile Ala Val Leu Glu Arg Ile
275 280 285Leu Ala Gln Ala Thr Ser Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp
290 295 300Ala Glu Ala Thr Gly Asn Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly305 310 315 320Ala Arg Ser Ala Glu Pro Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln
325 330 335Ala Leu Ala Lys Ala Thr Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg
340 345 350Asp Ile Leu Ala Gly Asn Val Glu Gly Leu Thr Val Asp Pro Asp Leu
355 360 365Arg Trp Trp Ala Leu Thr Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala
370 375 380Val Glu Asp Ala Ile Ala Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala385 390 395 400Ser Phe Leu Ala Ser Leu Arg Ala Gly Ala Ala Val Asn Thr Glu Glu
405 410 415Val Lys Ala Ala Ala Tyr Lys His Val Pro Ala Val Asp Ser Gly Leu
420 425 430Ser Asn Leu Glu Leu Arg His Lys Ile Glu Gly Leu Thr Phe Thr Gly
435 440 445Ser Phe Glu Leu Leu Gln Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu
450 455 460Asp Asp Val Trp Ala Asn Phe Ser Gly Glu Met Ala Gln Gln Ile Val465 470 475 480Leu Gly Leu Phe Pro Ser Trp Asn Val Ser Glu Glu Gly Leu Lys Arg
485 490 495Thr Asp Glu Phe Leu Asp Gly Glu His Val Ala Gly Ile Lys Arg Ile
500 505 510Val Ser Glu Ser Leu Asp Arg Thr Ala Arg Ala Leu Arg Asn
515 520 525<210>107<211>964<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(964)<223>FRXA01018<400>107tcttaaagtt ttctagcaat ccacactagg cgcgaactat cgtggtgtca ttgcgcacct 60tctaagggta gcgccccctc aaatttcaag gagcattaaa ttg acg tcc act aat 115
Leu Thr Ser Thr Asn
1 5ctc acc cga cag gaa gct tcg gat cgt tcg agg tta ctg agt gta gaa 163Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg Leu Leu Ser Val Glu
10 15 20aac tat gac att gca ctt gat ctc aac aac ggt gat gag ttt ttt agt 211Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly Asp Glu Phe Phe Ser
25 30 35tcc tcc acc gtt gtc agc ttc act gtc agg aag gct ggc gat acc ttt 259Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys Ala Gly Asp Thr Phe
40 45 50att gat ctc cgc gca gca agc gtt gag gag gtt cgc ctg gac aat gtg 307Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val Arg Leu Asp Asn Val
55 60 65tcc atc aaa gat gag gct cta acc ctt ggc aag aac ggc tac gac gag 355Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys Asn Gly Tyr Asp Glu70 75 80 85acg ttc ggc atc gcc ctg aag ggt ctt act ccc ggc gcg cac acc ttg 403Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro Gly Ala His Thr Leu
90 95 100cgg gta acg gcg tct atc ccc tat tcc cgc acc ggt gaa ggc ctg cac 451Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr Gly Glu Gly Leu His
105 110 115cgc atg gtg gat cca gca gac aat gag gtg tat ttg tac acc cag ttt 499Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr Leu Tyr Thr Gln Phe
120 125 130gag acc gcc gat gcc aag cgt atg ttc gcg tgt ttc gat cag cca gac 547Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys Phe Asp Gln Pro Asp
135 140 145ctc aag gct acc tat gat ctg aac atc aaa act cct aag ggt tgg aag 595Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr Pro Lys Gly Trp Lys150 155 160 165atc att tcc aac tct gag cag cag gtt tcc act cag cac act gat tac 643Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr Gln His Thr Asp Tyr
170 175 180gat acc cac att tcc cga gtg gac tat ccc ctc tcc acc tac ctg att 691Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu Ser Thr Tyr Leu Ile
185 190 195gcg gtg tgc gcg ggt cgt tac cac gag gtg tgc gat gtc tgg aag ggt 739Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys Asp Val Trp Lys Gly
200 205 210acg ctc acc cac cat gca gaa aca cct gcc gat cag cca act gag ctg 787Thr Leu Thr His His Ala Glu Thr Pro Ala Asp Gln Pro Thr Glu Leu
215 220 225act gtt ccg ctt gct ctc tac tgc cgc agt tct ttg gct aaa gat ctt 835Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser Leu Ala Lys Asp Leu230 235 240 245gat gcg gtg cgt ctg ttt acc gaa acg aag cag ggc ttt gat tgg tac 883Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln Gly Phe Asp Trp Tyr
250 255 260cac cgc aac ttc ggt gtg gcg tac cca ttc ggc aag tac gat cag atc 931His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly Lys Tyr Asp Gln Ile
265 270 275ttc gtc cct gaa ttt aat gct ggc gcg atg gag 964Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu
280 285<210>108<211>288<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>108Leu Thr Ser Thr Asn Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg1 5 10 15Leu Leu Ser Val Glu Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly
20 25 30Asp Glu Phe Phe Ser Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys
35 40 45Ala Gly Asp Thr Phe Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val
50 55 60Arg Leu Asp Asn Val Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys65 70 75 80Asn Gly Tyr Asp Glu Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro
85 90 95Gly Ala His Thr Leu Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr
100 105 110Gly Glu Gly Leu His Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr
115 120 125Leu Tyr Thr Gln Phe Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys
130 135 140Phe Asp Gln Pro Asp Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr145 150 155 160Pro Lys Gly Trp Lys Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr
165 170 175Gln His Thr Asp Tyr Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu
180 185 190Ser Thr Tyr Leu Ile Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys
195 200 205Asp Val Trp Lys Gly Thr Leu Thr His His Ala Glu Thr Pro Ala Asp
210 215 220Gln Pro Thr Glu Leu Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser225 230 235 240Leu Ala Lys Asp Leu Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln
245 250 255Gly Phe Asp Trp Tyr His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly
260 265 270Lys Tyr Asp Gln Ile Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu
275 280 285<210>109<211>1383<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1360)<223>RXA01147<400>109ataatgatca cctactttaa cggcttcagg tgacattgtg gattcgcatt gtggattcgg 60gggcccgcgc tgtttccaag aatttggcta cccttgttct atg cat gta act gac 115
Met His Val Thr Asp
1 5gat ttc tta agt ttt att gcc cta agc cca agt tcc tat cac gcg gcc 163Asp Phe Leu Ser Phe Ile Ala Leu Ser Pro Ser Ser Tyr His Ala Ala
10 15 20gcg gcg gtg gag cgc agg ttg ctc cat gag ggg ttc att cgt cag gaa 211Ala Ala Val Glu Arg Arg Leu Leu His Glu Gly Phe Ile Arg Gln Glu
25 30 35gat acc gat gaa tgg gat gcc cgc cct ggt ggg cat gtg acg gtg cgt 259Asp Thr Asp Glu Trp Asp Ala Arg Pro Gly Gly His Val Thr Val Arg
40 45 50ggg gga gca gta gtg gcg tgg tgg gtg cct gag gat gct tcg cca gat 307Gly Gly Ala Val Val Ala Trp Trp Val Pro Glu Asp Ala Ser Pro Asp
55 60 65tcc ggg ttc cgc atc att ggg tca cat act gat tca ccg ggt ttc aag 355Ser Gly Phe Arg Ile Ile Gly Ser His Thr Asp Ser Pro Gly Phe Lys70 75 80 85tta aag ccc cgt ggg gat ctt tcc tca cac ggt tgg cag cag gcc ggc 403Leu Lys Pro Arg Gly Asp Leu Ser Ser His Gly Trp Gln Gln Ala Gly
90 95 100gtc gag gtt tac ggc gga ccg atc ctg cca agc tgg ctg gat cgc gag 451Val Glu Val Tyr Gly Gly Pro Ile Leu Pro Ser Trp Leu Asp Arg Glu
105 110 115ctg gcc tta gca ggc cgc att gtg ctt gcc gac ggg tcc gtc aag ctt 499Leu Ala Leu Ala Gly Arg Ile Val Leu Ala Asp Gly Ser Val Lys Leu
120 125 130gtc aac acc ggc ccg att ctg cgc atc ccg cac gtg gct att cat ttg 547Val Asn Thr Gly Pro Ile Leu Arg Ile Pro His Val Ala Ile His Leu
135 140 145gac cgt act gtt aat tcc caa ctc acc ctt aat cca cag cgt cac ctg 595Asp Arg Thr Val Asn Ser Gln Leu Thr Leu Asn Pro Gln Arg His Leu150 155 160 165cag cct gtg ttt gct gtt ggt gag ccc gac gta tca att ctg gat gtc 643Gln Pro Val Phe Ala Val Gly Glu Pro Asp Val Ser Ile Leu Asp Val
170 175 180att gct ggt gct gcg gta gtg gat cct gca gat att gtc agc cat gat 691Ile Ala Gly Ala Ala Val Val Asp Pro Ala Asp Ile Val Ser His Asp
185 190 195ctg atc acg gtg gct acc caa gat gct gaa gta ttt ggc gca cat ggg 739Leu Ile Thr Val Ala Thr Gln Asp Ala Glu Val Phe Gly Ala His Gly
200 205 210gat ttc ttg gcg tct ggt cgc ctg gat aac ctg agc agc gtg cat cca 787Asp Phe Leu Ala Ser Gly Arg Leu Asp Asn Leu Ser Ser Val His Pro
215 220 225tcc atg act gca ttg att gcg gct tcg caa tct gac gat act ggt tcg 835Ser Met Thr Ala Leu Ile Ala Ala Ser Gln Ser Asp Asp Thr Gly Ser230 235 240 245gat att ttg gtt ctt gct gca ttc gat cat gaa gaa gtg gga agt aat 883Asp Ile Leu Val Leu Ala Ala Phe Asp His Glu Glu Val Gly Ser Asn
250 255 260tcc acc tcg ggt gcc ggg ggc ccc ctg ttg gag gat gtg ctc aac cgt 931Ser Thr Ser Gly Ala Gly Gly Pro Leu Leu Glu Asp Val Leu Asn Arg
265 270 275act gct cgt gcg ttg ggt gca gat gaa gat gag cga cgc cgg atg ttt 979Thr Ala Arg Ala Leu Gly Ala Asp Glu Asp Glu Arg Arg Arg Met Phe
280 285 290aac cgt tcc acc atg gtc tca gct gac gcg gca cac tcc att cac ccc 1027Asn Arg Ser Thr Met Val Ser Ala Asp Ala Ala His Ser Ile His Pro
295 300 305aac ttc ccc gag aag cat gat caa gct aat tac ccc atc att ggt aaa 1075Asn Phe Pro Glu Lys His Asp Gln Ala Asn Tyr Pro Ile Ile Gly Lys310 315 320 325ggt cct gta ttg aag gtc aac gcc aac cag cgc tac acc tcc gat gca 1123Gly Pro Val Leu Lys Val Asn Ala Asn Gln Arg Tyr Thr Ser Asp Ala
330 335 340gtc act tca ggc atg tgg atc agg gca tgt cag att gcc ggt gtg cca 1171Val Thr Ser Gly Met Trp Ile Arg Ala Cys Gln Ile Ala Gly Val Pro
345 350 355cac cag gtg ttt gcc ggc aac aac gat gtg ccg tgt ggt tcc acc atc 1219His Gln Val Phe Ala Gly Asn Asn Asp Val Pro Cys Gly Ser Thr Ile
360 365 370ggc ccg atc agt gcg act cgc ctg ggt atc gat tct gtc gat gtc ggt 1267Gly Pro Ile Ser Ala Thr Arg Leu Gly Ile Asp Ser Val Asp Val Gly
375 380 385att cca ttg ctg tcc atg cac tcc gca cgc gaa atg gcc gga gtg aag 1315Ile Pro Leu Leu Ser Met His Ser Ala Arg Glu Met Ala Gly Val Lys390 395 400 405gat ctg atg tgg ttt gaa caa gcc ctg gaa gcc tat ctg gta aat 1360Asp Leu Met Trp Phe Glu Gln Ala Leu Glu Ala Tyr Leu Val Asn
410 415 420taacgccgag ttcaatcaag aca 1383<210>110<211>420<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>110Met His Val Thr Asp Asp Phe Leu Ser Phe Ile Ala Leu Ser Pro Ser1 5 10 15Ser Tyr His Ala Ala Ala Ala Val Glu Arg Arg Leu Leu His Glu Gly
20 25 30Phe Ile Arg Gln Glu Asp Thr Asp Glu Trp Asp Ala Arg Pro Gly Gly
35 40 45His Val Thr Val Arg Gly Gly Ala Val Val Ala Trp Trp Val Pro Glu
50 55 60Asp Ala Ser Pro Asp Ser Gly Phe Arg Ile Ile Gly Ser His Thr Asp65 70 75 80Ser Pro Gly Phe Lys Leu Lys Pro Arg Gly Asp Leu Ser Ser His Gly
85 90 95Trp Gln Gln Ala Gly Val Glu Val Tyr Gly Gly Pro Ile Leu Pro Ser
100 105 110Trp Leu Asp Arg Glu Leu Ala Leu Ala Gly Arg Ile Val Leu Ala Asp
115 120 125Gly Ser Val Lys Leu Val Asn Thr Gly Pro Ile Leu Arg Ile Pro His
130 135 140Val Ala Ile His Leu Asp Arg Thr Val Asn Ser Gln Leu Thr Leu Asn145 150 155 160Pro Gln Arg His Leu Gln Pro Val Phe Ala Val Gly Glu Pro Asp Val
165 170 175Ser Ile Leu Asp Val Ile Ala Gly Ala Ala Val Val Asp Pro Ala Asp
180 185 190Ile Val Ser His Asp Leu Ile Thr Val Ala Thr Gln Asp Ala Glu Val
195 200 205Phe Gly Ala His Gly Asp Phe Leu Ala Ser Gly Arg Leu Asp Asn Leu
210 215 220Ser Ser Val His Pro Ser Met Thr Ala Leu Ile Ala Ala Ser Gln Ser225 230 235 240Asp Asp Thr Gly Ser Asp Ile Leu Val Leu Ala Ala Phe Asp His Glu
245 250 255Glu Val Gly Ser Asn Ser Thr Ser Gly Ala Gly Gly Pro Leu Leu Glu
260 265 270Asp Val Leu Asn Arg Thr Ala Arg Ala Leu Gly Ala Asp Glu Asp Glu
275 280 285Arg Arg Arg Met Phe Asn Arg Ser Thr Met Val Ser Ala Asp Ala Ala
290 295 300His Ser Ile His Pro Asn Phe Pro Glu Lys His Asp Gln Ala Asn Tyr305 310 315 320Pro Ile Ile Gly Lys Gly Pro Val Leu Lys Val Asn Ala Asn Gln Arg
325 330 335Tyr Thr Ser Asp Ala Val Thr Ser Gly Met Trp Ile Arg Ala Cys Gln
340 345 350Ile Ala Gly Val Pro His Gln Val Phe Ala Gly Asn Asn Asp Val Pro
355 360 365Cys Gly Ser Thr Ile Gly Pro Ile Ser Ala Thr Arg Leu Gly Ile Asp
370 375 380Ser Val Asp Val Gly Ile Pro Leu Leu Ser Met His Ser Ala Arg Glu385 390 395 400Met Ala Gly Val Lys Asp Leu Met Trp Phe Glu Gln Ala Leu Glu Ala
405 410 415Tyr Leu Val Asn
420<210>111<211>1260<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1237)<223>RXA01161<400>111tttttcgtga tcaacaatcc gctggcatag cgtccagcag atttgattct gacagtgtgg 60tttgatcgca cacctgccta ggctactagg gttggagact atg agt gat cct tca 115
Met Ser Asp Pro Ser
1 5aca aac aat ttc ccc aca tcg gta tat gca cag cgt ctt gcg gat gca 163Thr Asn Asn Phe Pro Thr Ser Val Tyr Ala Gln Arg Leu Ala Asp Ala
10 15 20caa gaa ggc gca cgc aag gct ggc ttg aac ggt ttg atc atc ggt aca 211Gln Glu Gly Ala Arg Lys Ala Gly Leu Asn Gly Leu Ile Ile Gly Thr
25 30 35ggc gca gaa ctt gcg tat cta acc ggc agc tgg atc tcc acc cat gag 259Gly Ala Glu Leu Ala Tyr Leu Thr Gly Ser Trp Ile Ser Thr His Glu
40 45 50cgt cta acc gct ttg gtg atc ccc agc gaa gga acc gca acc att gtt 307Arg Leu Thr Ala Leu Val Ile Pro Ser Glu Gly Thr Ala Thr Ile Val
55 60 65ctt ccc gct gta gac cgc gga gac tta gca ctg tct gct att cca gga 355Leu Pro Ala Val Asp Arg Gly Asp Leu Ala Leu Ser Ala Ile Pro Gly70 75 80 85cta gac atc aat gtg gcc gga tgg gtt gat ggc gat aat gcc cat gag 403Leu Asp Ile Asn Val Ala Gly Trp Val Asp Gly Asp Asn Ala His Glu
90 95 100ttg gcc gta gat gct ctc ggt gtt tca gag ttc gaa gca ttg ggt att 451Leu Ala Val Asp Ala Leu Gly Val Ser Glu Phe Glu Ala Leu Gly Ile
105 110 115ggt tcc tcc atc acg gca gat cac ctg att cct atc cag aac ctg gtg 499Gly Ser Ser Ile Thr Ala Asp His Leu Ile Pro Ile Gln Asn Leu Val
120 125 130ggc tcc acc tgc cgc atg gag ttg gca gtt caa gtg ctg aaa gaa ttg 547Gly Ser Thr Cys Arg Met Glu Leu Ala Val Gln Val Leu Lys Glu Leu
135 140 145ttt gtc tct aaa gac gaa gca gag atc gag cag ctt cgc ggc gca ggt 595Phe Val Ser Lys Asp Glu Ala Glu Ile Glu Gln Leu Arg Gly Ala Gly150 155 160 165gca gcc att gac cgt gtc cac gcc aaa gtc ccg gag ctt ctt caa gac 643Ala Ala Ile Asp Arg Val His Ala Lys Val Pro Glu Leu Leu Gln Asp
170 175 180gga cgc acc gaa gca gag gtt gca gca cag ctc aac gat ctc atc ttg 691Gly Arg Thr Glu Ala Glu Val Ala Ala Gln Leu Asn Asp Leu Ile Leu
185 190 195gaa gag cac tct gag gtg gac ttc gtg att gtg gga tcc gct gaa aac 739Glu Glu His Ser Glu Val Asp Phe Val Ile Val Gly Ser Ala Glu Asn
200 205 210ggc gcg aac cct cac cac ggt ttc tct gac cga gtc ctc cgc aat ggc 787Gly Ala Asn Pro His His Gly Phe Ser Asp Arg Val Leu Arg Asn Gly
215 220 225gac atc gtg gtg gtt gat ata gga ggc acc ttc ggc cct ggt tac cac 835Asp Ile Val Val Val Asp Ile Gly Gly Thr Phe Gly Pro Gly Tyr His230 235 240 245tct gac tgc aca cgc acc tac att gtg ggc gga aac cct gac gat gcg 883Ser Asp Cys Thr Arg Thr Tyr Ile Val Gly Gly Asn Pro Asp Asp Ala
250 255 260gat cca gag ttc gct aag ttc tac caa gtg ctc tac gaa gca cag ctc 931Asp Pro Glu Phe Ala Lys Phe Tyr Gln Val Leu Tyr Glu Ala Gln Leu
265 270 275gca gcc gtt gcg cat gtt cgc cct ggc gtt act gca gaa tca gtg gac 979Ala Ala Val Ala His Val Arg Pro Gly Val Thr Ala Glu Ser Val Asp
280 285 290gct gtt gct cgc gat cac att gct gcg gct gga tac ggc gaa tac ttc 1027Ala Val Ala Arg Asp His Ile Ala Ala Ala Gly Tyr Gly Glu Tyr Phe
295 300 305att cac cgc aca gga cac ggc att ggt cta tcc acc cat gag gag cca 1075Ile His Arg Thr Gly His Gly Ile Gly Leu Ser Thr His Glu Glu Pro310 315 320 325ttc atc atg gcg ggt aac tca ctc gtg ttg gaa gcc gga atg gcc ttt 1123Phe Ile Met Ala Gly Asn Ser Leu Val Leu Glu Ala Gly Met Ala Phe
330 335 340tcc att gag cct ggc atc tac att gaa gga atc cac gga gcg cgc atc 1171Ser Ile Glu Pro Gly Ile Tyr Ile Glu Gly Ile His Gly Ala Arg Ile
345 350 355gaa gac atc gtt gtg gtg aat gaa gac ggt tgt gaa acc ctc aac aac 1219Glu Asp Ile Val Val Val Asn Glu Asp Gly Cys Glu Thr Leu Asn Asn
360 365 370cag ccc aag gaa ctg cgt tgagcattct tctcctaggc gga 1260Gln Pro Lys Glu Leu Arg
375<210>112<211>379<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>112Met Ser Asp Pro Ser Thr Asn Asn Phe Pro Thr Ser Val Tyr Ala Gln1 5 10 15Arg Leu Ala Asp Ala Gln Glu Gly Ala Arg Lys Ala Gly Leu Asn Gly
20 25 30Leu Ile Ile Gly Thr Gly Ala Glu Leu Ala Tyr Leu Thr Gly Ser Trp
35 40 45Ile Ser Thr His Glu Arg Leu Thr Ala Leu Val Ile Pro Ser Glu Gly
50 55 60Thr Ala Thr Ile Val Leu Pro Ala Val Asp Arg Gly Asp Leu Ala Leu65 70 75 80Ser Ala Ile Pro Gly Leu Asp Ile Asn Val Ala Gly Trp Val Asp Gly
85 90 95Asp Asn Ala His Glu Leu Ala Val Asp Ala Leu Gly Val Ser Glu Phe
100 105 110Glu Ala Leu Gly Ile Gly Ser Ser Ile Thr Ala Asp His Leu Ile Pro
115 120 125Ile Gln Asn Leu Val Gly Ser Thr Cys Arg Met Glu Leu Ala Val Gln
130 135 140Val Leu Lys Glu Leu Phe Val Ser Lys Asp Glu Ala Glu Ile Glu Gln145 150 155 160Leu Arg Gly Ala Gly Ala Ala Ile Asp Arg Val His Ala Lys Val Pro
165 170 175Glu Leu Leu Gln Asp Gly Arg Thr Glu Ala Glu Val Ala Ala Gln Leu
180 185 190Asn Asp Leu Ile Leu Glu Glu His Ser Glu Val Asp Phe Val Ile Val
195 200 205Gly Ser Ala Glu Asn Gly Ala Asn Pro His His Gly Phe Ser Asp Arg
210 215 220Val Leu Arg Asn Gly Asp Ile Val Val Val Asp Ile Gly Gly Thr Phe225 230 235 240Gly Pro Gly Tyr His Ser Asp Cys Thr Arg Thr Tyr Ile Val Gly Gly
245 250 255Asn Pro Asp Asp Ala Asp Pro Glu Phe Ala Lys Phe Tyr Gln Val Leu
260 265 270Tyr Glu Ala Gln Leu Ala Ala Val Ala His Val Arg Pro Gly Val Thr
275 280 285Ala Glu Ser Val Asp Ala Val Ala Arg Asp His Ile Ala Ala Ala Gly
290 295 300Tyr Gly Glu Tyr Phe Ile His Arg Thr Gly His Gly Ile Gly Leu Ser305 310 315 320Thr His Glu Glu Pro Phe Ile Met Ala Gly Asn Ser Leu Val Leu Glu
325 330 335Ala Gly Met Ala Phe Ser Ile Glu Pro Gly Ile Tyr Ile Glu Gly Ile
340 345 350His Gly Ala Arg Ile Glu Asp Ile Val Val Val Asn Glu Asp Gly Cys
355 360 365Glu Thr Leu Asn Asn Gln Pro Lys Glu Leu Arg
370 375<210>113<211>980<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(957)<223>RXN01181<400>113tct gta ctg ctc gct cgc gac ttg gtg aac acc cct tca tca cac ctg 48Ser Val Leu Leu Ala Arg Asp Leu Val Asn Thr Pro Ser Ser His Leu1 5 10 15tac cca gag tcc tac tca gta att gca tcc aac gaa gcg tcc aag cac 96Tyr Pro Glu Ser Tyr Ser Val Ile Ala Ser Asn Glu Ala Ser Lys His
20 25 30ggc ttg cag acc acc atc ctg gat gag aag cag ctt gct gat caa ggt 144Gly Leu Gln Thr Thr Ile Leu Asp Glu Lys Gln Leu Ala Asp Gln Gly
35 40 45ttc ggc ggc atc ctc gca gtc ggt aac ggc tcc tcc cgc aag cct cgt 192Phe Gly Gly Ile Leu Ala Val Gly Asn Gly Ser Ser Arg Lys Pro Arg
50 55 60ctg ctg cgc atc gat tgg aag cca cgc aag gct aag aag tcg atc gct 240Leu Leu Arg Ile Asp Trp Lys Pro Arg Lys Ala Lys Lys Ser Ile Ala65 70 75 80ttg gtt ggc aag ggc atc acc ttt gac acc ggc gga att tcc atc aag 288Leu Val Gly Lys Gly Ile Thr Phe Asp Thr Gly Gly Ile Ser Ile Lys
85 90 95cct ggc gca agc atg gag aac atg atc tcc gac atg ggt gga tcc gca 336Pro Gly Ala Ser Met Glu Asn Met Ile Ser Asp Met Gly Gly Ser Ala
100 105 110tcc gta ttg gcc acc att atc gct gca gct cgt ttg aac ctg tcg atc 384Ser Val Leu Ala Thr Ile Ile Ala Ala Ala Arg Leu Asn Leu Ser Ile
115 120 125aac gtc ttc gcg ttc cta cca atg gct gag aac atg cca tcc ggt gac 432Asn Val Phe Ala Phe Leu Pro Met Ala Glu Asn Met Pro Ser Gly Asp
130 135 140gct ttc cgc ccc ggc gat gtc atc act cat ttc ggt ggt atc acc tcc 480Ala Phe Arg Pro Gly Asp Val Ile Thr His Phe Gly Gly Ile Thr Ser145 150 155 160gaa atc ttg aac acc gac gct gaa ggc cgc ctc att ctg gca gat gcc 528Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg Leu Ile Leu Ala Asp Ala
165 170 175att gct tac gct tct gaa gat aag cct gac tac ctc att gat gcg gca 576Ile Ala Tyr Ala Ser Glu Asp Lys Pro Asp Tyr Leu Ile Asp Ala Ala
180 185 190acc ctg act ggt gct caa tta gtc gct tta ggc ctg cgg act tca ggt 624Thr Leu Thr Gly Ala Gln Leu Val Ala Leu Gly Leu Arg Thr Ser Gly
195 200 205gtc atg ggt acc gat gag ttc cgc gac agc gtt gcc aag act ggc cgc 672Val Met Gly Thr Asp Glu Phe Arg Asp Ser Val Ala Lys Thr Gly Arg
210 215 220gag gtt ggc gag caa gca tgg gca atg cct ctt cct gaa gag ctc gat 720Glu Val Gly Glu Gln Ala Trp Ala Met Pro Leu Pro Glu Glu Leu Asp225 230 235 240gag cag gtt aag tcc cct gtc gct gac ctg cgc aat gtc acc aat tcc 768Glu Gln Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val Thr Asn Ser
245 250 255cgt ttc gca gga atg tct gct gcg ggt cgt tac ttg cag gaa ttc gtt 816Arg Phe Ala Gly Met Ser Ala Ala Gly Arg Tyr Leu Gln Glu Phe Val
260 265 270ggt gcc gac atc gag tgg gct cac gtc gat atc gct ggc cct gca tac 864Gly Ala Asp Ile Glu Trp Ala His Val Asp Ile Ala Gly Pro Ala Tyr
275 280 285aac act gct ggt gaa ttc ggt tac acg cca aag cgc gca acc gga caa 912Asn Thr Ala Gly Glu Phe Gly Tyr Thr Pro Lys Arg Ala Thr Gly Gln
290 295 300cca gtg cgc acc ttc gtt cag gtt ctg aag gat ctg tcg gaa agc 957Pro Val Arg Thr Phe Val Gln Val Leu Lys Asp Leu Ser Glu Ser305 310 315taaacgctag ttaaagatca gga 980<210>114<211>319<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>114Ser Val Leu Leu Ala Arg Asp Leu Val Asn Thr Pro Ser Ser His Leu1 5 10 15Tyr Pro Glu Ser Tyr Ser Val Ile Ala Ser Asn Glu Ala Ser Lys His
20 25 30Gly Leu Gln Thr Thr Ile Leu Asp Glu Lys Gln Leu Ala Asp Gln Gly
35 40 45Phe Gly Gly Ile Leu Ala Val Gly Asn Gly Ser Ser Arg Lys Pro Arg
50 55 60Leu Leu Arg Ile Asp Trp Lys Pro Arg Lys Ala Lys Lys Ser Ile Ala65 70 75 80Leu Val Gly Lys Gly Ile Thr Phe Asp Thr Gly Gly Ile Ser Ile Lys
85 90 95Pro Gly Ala Ser Met Glu Asn Met Ile Ser Asp Met Gly Gly Ser Ala
100 105 110Ser Val Leu Ala Thr Ile Ile Ala Ala Ala Arg Leu Asn Leu Ser Ile
115 120 125Asn Val Phe Ala Phe Leu Pro Met Ala Glu Asn Met Pro Ser Gly Asp
130 135 140Ala Phe Arg Pro Gly Asp Val Ile Thr His Phe Gly Gly Ile Thr Ser145 150 155 160Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg Leu Ile Leu Ala Asp Ala
165 170 175Ile Ala Tyr Ala Ser Glu Asp Lys Pro Asp Tyr Leu Ile Asp Ala Ala
180 185 190Thr Leu Thr Gly Ala Gln Leu Val Ala Leu Gly Leu Arg Thr Ser Gly
195 200 205Val Met Gly Thr Asp Glu Phe Arg Asp Ser Val Ala Lys Thr Gly Arg
210 215 220Glu Val Gly Glu Gln Ala Trp Ala Met Pro Leu Pro Glu Glu Leu Asp225 230 235 240Glu Gln Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val Thr Asn Ser
245 250 255Arg Phe Ala Gly Met Ser Ala Ala Gly Arg Tyr Leu Gln Glu Phe Val
260 265 270Gly Ala Asp Ile Glu Trp Ala His Val Asp Ile Ala Gly Pro Ala Tyr
275 280 285Asn Thr Ala Gly Glu Phe Gly Tyr Thr Pro Lys Arg Ala Thr Gly Gln
290 295 300Pro Val Arg Thr Phe Val Gln Val Leu Lys Asp Leu Ser Glu Ser305 310 315<210>115<211>980<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(957)<223>FRXA01181<400>115tct gta ctg ctc gct cgc gac ttg gtg aac acc cct tca tca cac ctg 48Ser Val Leu Leu Ala Arg Asp Leu Val Asn Thr Pro Ser Ser His Leu1 5 10 15tac cca gag tcc tac tca gta att gca tcc aac gaa gcg tcc aag cac 96Tyr Pro Glu Ser Tyr Ser Val Ile Ala Ser Asn Glu Ala Ser Lys His
20 25 30ggc ttg cag acc acc atc ctg gat gag aag cag ctt gct gat caa ggt 144Gly Leu Gln Thr Thr Ile Leu Asp Glu Lys Gln Leu Ala Asp Gln Gly
35 40 45ttc ggc ggc atc ctc gca gtc ggt aac ggc tcc tcc cgc aag cct cgt 192Phe Gly Gly Ile Leu Ala Val Gly Asn Gly Ser Ser Arg Lys Pro Arg
50 55 60ctg ctg cgc atc gat tgg aag cca cgc aag gct aag aag tcg atc gct 240Leu Leu Arg Ile Asp Trp Lys Pro Arg Lys Ala Lys Lys Ser Ile Ala65 70 75 80ttg gtt ggc aag ggc atc acc ttt gac acc ggc gga att tcc atc aag 288Leu Val Gly Lys Gly Ile Thr Phe Asp Thr Gly Gly Ile Ser Ile Lys
85 90 95cct ggc gca agc atg gag aac atg atc tcc gac atg ggt gga tcc gca 336Pro Gly Ala Ser Met Glu Asn Met Ile Ser Asp Met Gly Gly Ser Ala
100 105 110tcc gta ttg gcc acc att atc gct gca gct cgt ttg aac ctg tcg atc 384Ser Val Leu Ala Thr Ile Ile Ala Ala Ala Arg Leu Asn Leu Ser Ile
115 120 125aac gtc ttc gcg ttc cta cca atg gct gag aac atg cca tcc ggt gac 432Asn Val Phe Ala Phe Leu Pro Met Ala Glu Asn Met Pro Ser Gly Asp
130 135 140gct ttc cgc ccc ggc gat gtc atc act cat ttc ggt ggt atc acc tcc 480Ala Phe Arg Pro Gly Asp Val Ile Thr His Phe Gly Gly Ile Thr Ser145 150 155 160gaa atc ttg aac acc gac gct gaa ggc cgc ctc att ctg gca gat gcc 528Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg Leu Ile Leu Ala Asp Ala
165 170 175att gct tac gct tct gaa gat aag cct gac tac ctc att gat gcg gca 576Ile Ala Tyr Ala Ser Glu Asp Lys Pro Asp Tyr Leu Ile Asp Ala Ala
180 185 190acc ctg act ggt gct caa tta gtc gct tta ggc ctg cgg act tca ggt 624Thr Leu Thr Gly Ala Gln Leu Val Ala Leu Gly Leu Arg Thr Ser Gly
195 200 205gtc atg ggt acc gat gag ttc cgc gac agc gtt gcc aag act ggc cgc 672Val Met Gly Thr Asp Glu Phe Arg Asp Ser Val Ala Lys Thr Gly Arg
210 215 220gag gtt ggc gag caa gca tgg gca atg cct ctt cct gaa gag ctc gat 720Glu Val Gly Glu Gln Ala Trp Ala Met Pro Leu Pro Glu Glu Leu Asp225 230 235 240gag cag gtt aag tcc cct gtc gct gac ctg cgc aat gtc acc aat tcc 768Glu Gln Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val Thr Asn Ser
245 250 255cgt ttc gca gga atg tct gct gcg ggt cgt tac ttg cag gaa ttc gtt 816Arg Phe Ala Gly Met Ser Ala Ala Gly Arg Tyr Leu Gln Glu Phe Val
260 265 270ggt gcc gac atc gag tgg gct cac gtc gat atc gct ggc cct gca tac 864Gly Ala Asp Ile Glu Trp Ala His Val Asp Ile Ala Gly Pro Ala Tyr
275 280 285aac act gct ggt gaa ttc ggt tac acg cca aag cgc gca acc gga caa 912Asn Thr Ala Gly Glu Phe Gly Tyr Thr Pro Lys Arg Ala Thr Gly Gln
290 295 300cca gtg cgc acc ttc gtt cag gtt ctg aag gat ctg tcg gaa agc 957Pro Val Arg Thr Phe Val Gln Val Leu Lys Asp Leu Ser Glu Ser305 310 315taaacgctag ttaaagatca gga 980<210>116<211>319<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>116Ser Val Leu Leu Ala Arg Asp Leu Val Asn Thr Pro Ser Ser His Leu1 5 10 15Tyr Pro Glu Ser Tyr Ser Val Ile Ala Ser Asn Glu Ala Ser Lys His
20 25 30Gly Leu Gln Thr Thr Ile Leu Asp Glu Lys Gln Leu Ala Asp Gln Gly
35 40 45Phe Gly Gly Ile Leu Ala Val Gly Asn Gly Ser Ser Arg Lys Pro Arg
50 55 60Leu Leu Arg Ile Asp Trp Lys Pro Arg Lys Ala Lys Lys Ser Ile Ala65 70 75 80Leu Val Gly Lys Gly Ile Thr Phe Asp Thr Gly Gly Ile Ser Ile Lys
85 90 95Pro Gly Ala Ser Met Glu Asn Met Ile Ser Asp Met Gly Gly Ser Ala
100 105 110Ser Val Leu Ala Thr Ile Ile Ala Ala Ala Arg Leu Asn Leu Ser Ile
115 120 125Asn Val Phe Ala Phe Leu Pro Met Ala Glu Asn Met Pro Ser Gly Asp
130 135 140Ala Phe Arg Pro Gly Asp Val Ile Thr His Phe Gly Gly Ile Thr Ser145 150 155 160Glu Ile Leu Asn Thr Asp Ala Glu Gly Arg Leu Ile Leu Ala Asp Ala
165 170 175Ile Ala Tyr Ala Ser Glu Asp Lys Pro Asp Tyr Leu Ile Asp Ala Ala
180 185 190Thr Leu Thr Gly Ala Gln Leu Val Ala Leu Gly Leu Arg Thr Ser Gly
195 200 205Val Met Gly Thr Asp Glu Phe Arg Asp Ser Val Ala Lys Thr Gly Arg
210 215 220Glu Val Gly Glu Gln Ala Trp Ala Met Pro Leu Pro Glu Glu Leu Asp225 230 235 240Glu Gln Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val Thr Asn Ser
245 250 255Arg Phe Ala Gly Met Ser Ala Ala Gly Arg Tyr Leu Gln Glu Phe Val
260 265 270Gly Ala Asp Ile Glu Trp Ala His Val Asp Ile Ala Gly Pro Ala Tyr
275 280 285Asn Thr Ala Gly Glu Phe Gly Tyr Thr Pro Lys Arg Ala Thr Gly Gln
290 295 300Pro Val Arg Thr Phe Val Gln Val Leu Lys Asp Leu Ser Glu Ser305 310 315<210>117<211>2127<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(2104)<223>RXN01277<400>117tactactcgg ttcacgttta cgtcggctga tccaattgga ggcgccctcg gaagccgcct 60taaaaaacct gccggtcaaa agatcactaa cctgaacttc atg act gat tac acg 115
Met Thr Asp Tyr Thr
1 5ttc ctc gaa gac att gac acc ccg gaa gcg ctc gcg tgg gcg gaa aaa 163Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala Leu Ala Trp Ala Glu Lys
10 15 20tgg tcg ggg gaa agc gtc gaa aag cta aaa agc cca gcc aag gac gcc 211Trp Ser Gly Glu Ser Val Glu Lys Leu Lys Ser Pro Ala Lys Asp Ala
25 30 35ctg gaa gcc agg ctg ctg gct gcg ttg gac acc gat gat cgc att gcc 259Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp Thr Asp Asp Arg Ile Ala
40 45 50tac gtg agc cgg cgc ggt gag aag ctg tac aac ttt tgg cgg gac gcg 307Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr Asn Phe Trp Arg Asp Ala
55 60 65cag cat ccg cgt gga gtg tgg cgc acg acc acg ttg gag tcg tat gaa 355Gln His Pro Arg Gly Val Trp Arg Thr Thr Thr Leu Glu Ser Tyr Glu70 75 80 85agt gac cag ccg gag tgg gac gtg ctc att gat gtg gat gcg ttg gcg 403Ser Asp Gln Pro Glu Trp Asp Val Leu Ile Asp Val Asp Ala Leu Ala
90 95 100gag gat gag ggc gaa aac tgg gta tgg aag ggc gcg gtt gtg cgc tcg 451Glu Asp Glu Gly Glu Asn Trp Val Trp Lys Gly Ala Val Val Arg Ser
105 110 115ccg gag ttt gat cgg gcg ttg gtg aag ttc tcg cgg ggc ggg gct gat 499Pro Glu Phe Asp Arg Ala Leu Val Lys Phe Ser Arg Gly Gly Ala Asp
120 125 130gcg acg gtg att agg gag ttt gat ctg gcc acg gct gct ttc gtg gat 547Ala Thr Val Ile Arg Glu Phe Asp Leu Ala Thr Ala Ala Phe Val Asp
135 140 145gat tcg ccg ttt gaa ttg aag gag gcg aag tcc gat gtc acg tgg gtt 595Asp Ser Pro Phe Glu Leu Lys Glu Ala Lys Ser Asp Val Thr Trp Val150 155 160 165gat ctg gat acg ttg ctg gtg ggc acg gat acc ggc gag ggg tca ctg 643Asp Leu Asp Thr Leu Leu Val Gly Thr Asp Thr Gly Glu Gly Ser Leu
170 175 180acg gat tct ggg tac ccg gcg egg gtg ctc acg tgg aag cgt ggg act 691Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu Thr Trp Lys Arg Gly Thr
185 190 195ccg ctt gag cag gcg gag ttg ttc ttt gag ggg tcg cgt cag gat gtg 739Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu Gly Ser Arg Gln Asp Val
200 205 210gcg act cat gcg tgg cgg gat tca aca cct ggt ttt gag cgg acg ttt 787Ala Thr His Ala Trp Arg Asp Ser Thr Pro Gly Phe Glu Arg Thr Phe
215 220 225gtg tca agg tcg ttg gat ttc tat aat tcg gag acg tcg ctg gaa acc 835Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser Glu Thr Ser Leu Glu Thr230 235 240 245gag ggt ggc ctg gtc aag ctt gat gtg ccg acc gat tgc gat gtc att 883Glu Gly Gly Leu Val Lys Leu Asp Val Pro Thr Asp Cys Asp Val Ile
250 255 260gtg aag aag cag tgg att ttt gtg agt cct cgg acg gat ttc gct ggg 931Val Lys Lys Gln Trp Ile Phe Val Ser Pro Arg Thr Asp Phe Ala Gly
265 270 275att cca gca ggt ggc ttg gga gtg ctg ctg tta aag gag ttc ctt gag 979Ile Pro Ala Gly Gly Leu Gly Val Leu Leu Leu Lys Glu Phe Leu Glu
280 285 290ggc ggg cgc gat ttt cag cct gtg ttt acg cct act gag tcg acg tcg 1027Gly Gly Arg Asp Phe Gln Pro Val Phe Thr Pro Thr Glu Ser Thr Ser
295 300 305ctg cag gga ttg gcc acg aca aag aat ttc ctg gtt tta acg ctc ctt 1075Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe Leu Val Leu Thr Leu Leu310 315 320 325aat aat gtc tcc aca gaa atc gtc aca gtg ccg ctc aat gat ccg aca 1123Asn Asn Val Ser Thr Glu Ile Val Thr Val Pro Leu Asn Asp Pro Thr
330 335 340acg gag cat gaa cac att gac ctc cca gag cat gtc acc gcg cat gtg 1171Thr Glu His Glu His Ile Asp Leu Pro Glu His Val Thr Ala His Val
345 350 355gtt gct acc tcc ccg ttg gat ggc gat gaa att tgg gtg cag gca gcg 1219Val Ala Thr Ser Pro Leu Asp Gly Asp Glu Ile Trp Val Gln Ala Ala
360 365 370agt ttc acc gaa gcg cca acg ttg ctg cgt gcg gag ctg cct ggt gcg 1267Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg Ala Glu Leu Pro Gly Ala
375 380 385ctt gag gct gtg aag aag gcg ccg ttg cag ttt gaa aat gct ggt cag 1315Leu Glu Ala Val Lys Lys Ala Pro Leu Gln Phe Glu Asn Ala Gly Gln390 395 400 405gag act cgt cag cat tgg gca acc tcg gcg gat gga acg aag att ccg 1363Glu Thr Arg Gln His Trp Ala Thr Ser Ala Asp Gly Thr Lys Ile Pro
410 415 420tac ttt att aca gga gcc ttc gag gag gaa cca caa aac acc ctg gtc 1411Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu Pro Gln Asn Thr Leu Val
425 430 435cac gcc tac ggc ggc ttc gag gtt tcc ctt acc cca agc cac tcc ccg 1459His Ala Tyr Gly Gly Phe Glu Val Ser Leu Thr Pro Ser His Ser Pro
440 445 450acc cgc ggc atc gca tgg ttg gaa aag ggc tac tac ttt gtg gaa gcc 1507Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly Tyr Tyr Phe Val Glu Ala
455 460 465aac ctg cgt ggt ggc ggt gaa ttc ggt ccg gaa tgg cat tcg cag gca 1555Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro Glu Trp His Ser Gln Ala470 475 480 485acc aag ctg aac cgc atg aag gtg tgg gag gat cac cgc gcg gtg ctc 1603Thr Lys Leu Asn Arg Met Lys Val Trp Glu Asp His Arg Ala Val Leu
490 495 500gcc gac ctt gtg gag cgc ggc tac gca acg ccg gag cag att gcg att 1651Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr Pro Glu Gln Ile Ala Ile
505 510 515cgt ggc gga tcc aac ggt ggt ttg ctg aca agt ggc gcg tta act cag 1699Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr Ser Gly Ala Leu Thr Gln
520 525 530tac cca gaa gca ttc ggt gcg gca gtt gtg cag gtg ccg ttg gct gat 1747Tyr Pro Glu Ala Phe Gly Ala Ala Val Val Gln Val Pro Leu Ala Asp
535 540 545atg ttg cgc tat cac acc tgg tca gcg ggt gct tcg tgg atg gcg gag 1795Met Leu Arg Tyr His Thr Trp Ser Ala Gly Ala Ser Trp Met Ala Glu550 555 560 565tac ggc aac cct gac gat ccg gag gaa cgg gcg gtg att gag cag tac 1843Tyr Gly Asn Pro Asp Asp Pro Glu Glu Arg Ala Val Ile Glu Gln Tyr
570 575 580tcg ccg gtg cag gcg gtg gtg ggc gtc gag aag cga att tat cca ccc 1891Ser Pro Val Gln Ala Val Val Gly Val Glu Lys Arg Ile Tyr Pro Pro
585 590 595gca ttg gtg acg acc tca acc cgg gac gac cgc gtc cac ccc gcg cac 1939Ala Leu Val Thr Thr Ser Thr Arg Asp Asp Arg Val His Pro Ala His
600 605 610gcg cgc ctt ttt gct caa gct ttg ctt gat gcg ggc cag gcc gtg gat 1987Ala Arg Leu Phe Ala Gln Ala Leu Leu Asp Ala Gly Gln Ala Val Asp
615 620 625tac tac gaa aac acc gag ggc ggc cat gcc ggc gcg gcg gat aac aag 2035Tyr Tyr Glu Asn Thr Glu Gly Gly His Ala Gly Ala Ala Asp Asn Lys630 635 640 645cag acc gcg ttt gtg gaa tcg ctg atc tac acc tgg atc gag aag act 2083Gln Thr Ala Phe Val Glu Ser Leu Ile Tyr Thr Trp Ile Glu Lys Thr
650 655 660ttg gat cag cag ggt agc att taatacctat gattatgcga agg 2127Leu Asp Gln Gln Gly Ser Ile
665<210>118<211>668<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>118Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala Leu1 5 10 15Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser Val Glu Lys Leu Lys Ser
20 25 30Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp Thr
35 40 45Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr Asn
50 55 60Phe Trp Arg Asp Ala Gln His Pro Arg Gly Val Trp Arg Thr Thr Thr65 70 75 80Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu Trp Asp Val Leu Ile Asp
85 90 95Val Asp Ala Leu Ala Glu Asp Glu Gly Glu Asn Trp Val Trp Lys Gly
100 105 110Ala Val Val Arg Ser Pro Glu Phe Asp Arg Ala Leu Val Lys Phe Ser
115 120 125Arg Gly Gly Ala Asp Ala Thr Val Ile Arg Glu Phe Asp Leu Ala Thr
130 135 140Ala Ala Phe Val Asp Asp Ser Pro Phe Glu Leu Lys Glu Ala Lys Ser145 150 155 160Asp Val Thr Trp Val Asp Leu Asp Thr Leu Leu Val Gly Thr Asp Thr
165 170 175Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu Thr
180 185 190Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu Gly
195 200 205Ser Arg Gln Asp Val Ala Thr His Ala Trp Arg Asp Ser Thr Pro Gly
210 215 220Phe Glu Arg Thr Phe Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser Glu225 230 235 240Thr Ser Leu Glu Thr Glu Gly Gly Leu Val Lys Leu Asp Val Pro Thr
245 250 255Asp Cys Asp Val Ile Val Lys Lys Gln Trp Ile Phe Val Ser Pro Arg
260 265 270Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly Leu Gly Val Leu Leu Leu
275 280 285Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe Gln Pro Val Phe Thr Pro
290 295 300Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe Leu305 310 315 320Val Leu Thr Leu Leu Asn Asn Val Ser Thr Glu Ile Val Thr Val Pro
325 330 335Leu Asn Asp Pro Thr Thr Glu His Glu His Ile Asp Leu Pro Glu His
340 345 350Val Thr Ala His Val Val Ala Thr Ser Pro Leu Asp Gly Asp Glu Ile
355 360 365Trp Val Gln Ala Ala Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg Ala
370 375 380Glu Leu Pro Gly Ala Leu Glu Ala Val Lys Lys Ala Pro Leu Gln Phe385 390 395 400Glu Asn Ala Gly Gln Glu Thr Arg Gln His Trp Ala Thr Ser Ala Asp
405 410 415Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu Pro
420 425 430Gln Asn Thr Leu Val His Ala Tyr Gly Gly Phe Glu Val Ser Leu Thr
435 440 445Pro Ser His Ser Pro Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly Tyr
450 455 460Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro Glu465 470 475 480Trp His Ser Gln Ala Thr Lys Leu Asn Arg Met Lys Val Trp Glu Asp
485 490 495His Arg Ala Val Leu Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr Pro
500 505 510Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr Ser
515 520 525Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe Gly Ala Ala Val Val Gln
530 535 540Val Pro Leu Ala Asp Met Leu Arg Tyr His Thr Trp Ser Ala Gly Ala545 550 555 560Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp Asp Pro Glu Glu Arg Ala
565 570 575Val Ile Glu Gln Tyr Ser Pro Val Gln Ala Val Val Gly Val Glu Lys
580 585 590Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr Ser Thr Arg Asp Asp Arg
595 600 605Val His Pro Ala His Ala Arg Leu Phe Ala Gln Ala Leu Leu Asp Ala
610 615 620Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr Glu Gly Gly His Ala Gly625 630 635 640Ala Ala Asp Asn Lys Gln Thr Ala Phe Val Glu Ser Leu Ile Tyr Thr
645 650 655Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly Ser Ile
660 665<210>119<211>1812<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1789)<223>FRXA01277<400>119tactactcgg ttcacgttta cgtcggctga tccaattgga ggcgccctcg gaagccgcct 60taaaaaacct gccggtcaaa agatcactaa cctgaacttc atg act gat tac acg 115
Met Thr Asp Tyr Thr
1 5ttc ctc gaa gac att gac acc ccg gaa gcg ctc gcg tgg gcg gaa aaa 163Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala Leu Ala Trp Ala Glu Lys
10 15 20tgg tcg ggg gaa agc gtc gaa aag cta aaa agc cca gcc aag gac gcc 211Trp Ser Gly Glu Ser Val Glu Lys Leu Lys Ser Pro Ala Lys Asp Ala
25 30 35ctg gaa gcc agg ctg ctg gct gcg ttg gac acc gat gat cgc att gcc 259Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp Thr Asp Asp Arg Ile Ala
40 45 50tac gtg agc cgg cgc ggt gag aag ctg tac aac ttt tgg cgg gac gcg 307Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr Asn Phe Trp Arg Asp Ala
55 60 65cag cat ccg cgt gga gtg tgg cgc acg acc acg ttg gag tcg tat gaa 355Gln His Pro Arg Gly Val Trp Arg Thr Thr Thr Leu Glu Ser Tyr Glu70 75 80 85agt gac cag ccg gag tgg gac gtg ctc att gat gtg gat gcg ttg gcg 403Ser Asp Gln Pro Glu Trp Asp Val Leu Ile Asp Val Asp Ala Leu Ala
90 95 100gag gat gag ggc gaa aac tgg gta tgg aag ggc gcg gtt gtg cgc tcg 451Glu Asp Glu Gly Glu Asn Trp Val Trp Lys Gly Ala Val Val Arg Ser
105 110 115ccg gag ttt gat cgg gcg ttg gtg aag ttc tcg cgg ggc ggg gct gat 499Pro Glu Phe Asp Arg Ala Leu Val Lys Phe Ser Arg Gly Gly Ala Asp
120 125 130gcg acg gtg att agg gag ttt gat ctg gcc acg gct gct ttc gtg gat 547Ala Thr Val Ile Arg Glu Phe Asp Leu Ala Thr Ala Ala Phe Val Asp
135 140 145gat tcg ccg ttt gaa ttg aag gag gcg aag tcc gat gtc acg tgg gtt 595Asp Ser Pro Phe Glu Leu Lys Glu Ala Lys Ser Asp Val Thr Trp Val150 155 160 165gat ctg gat acg ttg ctg gtg ggc acg gat acc ggc gag ggg tca ctg 643Asp Leu Asp Thr Leu Leu Val Gly Thr Asp Thr Gly Glu Gly Ser Leu
170 175 180acg gat tct ggg tac ccg gcg cgg gtg ctc acg tgg aag cgt ggg act 691Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu Thr Trp Lys Arg Gly Thr
185 190 195ccg ctt gag cag gcg gag ttg ttc ttt gag ggg tcg cgt cag gat gtg 739Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu Gly Ser Arg Gln Asp Val
200 205 210gcg act cat gcg tgg cgg gat tca aca cct ggt ttt gag cgg acg ttt 787Ala Thr His Ala Trp Arg Asp Ser Thr Pro Gly Phe Glu Arg Thr Phe
215 220 225gtg tca agg tcg ttg gat ttc tat aat tcg gag acg tcg ctg gaa acc 835Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser Glu Thr Ser Leu Glu Thr230 235 240 245gag ggt ggc ctg gtc aag ctt gat gtg ccg acc gat tgc gat gtc att 883Glu Gly Gly Leu Val Lys Leu Asp Val Pro Thr Asp Cys Asp Val Ile
250 255 260gtg aag aag cag tgg att ttt gtg agt cct cgg acg gat ttc gct ggg 931Val Lys Lys Gln Trp Ile Phe Val Ser Pro Arg Thr Asp Phe Ala Gly
265 270 275att cca gca ggt ggc ttg gga gtg ctg ctg tta aag gag ttc ctt gag 979Ile Pro Ala Gly Gly Leu Gly Val Leu Leu Leu Lys Glu Phe Leu Glu
280 285 290ggc ggg cgc gat ttt cag cct gtg ttt acg cct act gag tcg acg tcg 1027Gly Gly Arg Asp Phe Gln Pro Val Phe Thr Pro Thr Glu Ser Thr Ser
295 300 305ctg cag gga ttg gcc acg aca aag aat ttc ctg gtt tta acg ctc ctt 1075Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe Leu Val Leu Thr Leu Leu310 315 320 325aat aat gtc tcc aca gaa atc gtc aca gtg ccg ctc aat gat ccg aca 1123Asn Asn Val Ser Thr Glu Ile Val Thr Val Pro Leu Asn Asp Pro Thr
330 335 340acg gag cat gaa cac att gac ctc cca gag cat gtc acc gcg cat gtg 1171Thr Glu His Glu His Ile Asp Leu Pro Glu His Val Thr Ala His Val
345 350 355gtt gct acc tcc ccg ttg gat ggc gat gaa att tgg gtg cag gca gcg 1219Val Ala Thr Ser Pro Leu Asp Gly Asp Glu Ile Trp Val Gln Ala Ala
360 365 370agt ttc acc gaa gcg cca acg ttg ctg cgt gcg gag ctg cct ggt gcg 1267Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg Ala Glu Leu Pro Gly Ala
375 380 385ctt gag gct gtg aag aag gcg ccg ttg cag ttt gaa aat gct ggt cag 1315Leu Glu Ala Val Lys Lys Ala Pro Leu Gln Phe Glu Asn Ala Gly Gln
395 400 405gag act cgt cag cat tgg gca acc tcg gcg gat gga acg aag att ccg 1363Glu Thr Arg Gln His Trp Ala Thr Ser Ala Asp Gly Thr Lys Ile Pro
410 415 420tac ttt att aca gga gcc ttc gag gag gaa cca caa aac acc ctg gtc 1411Tyr Phe lle Thr Gly Ala Phe Glu Glu Glu Pro Gln Asn Thr Leu Val
425 430 435cac gcc tac ggc ggc ttc gag gtt tcc ctt acc cca agc cac tcc ccg 1459His Ala Tyr Gly Gly Phe Glu Val Ser Leu Thr Pro Ser His Ser Pro
440 445 450acc cgc ggc atc gca tgg ttg gaa aag ggc tac tac ttt gtg gaa gcc 1507Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly Tyr Tyr Phe Val Glu Ala
455 460 465aac ctg cgt ggt ggc ggt gaa ttc ggt ccg gaa tgg cat tcg cag gca 1555Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro Glu Trp His Ser Gln Ala470 475 480 485acc aag ctg aac cgc atg aag gtg tgg gag gat cac cgc gcg gtg ctc 1603Thr Lys Leu Asn Arg Met Lys Val Trp Glu Asp His Arg Ala Val Leu
490 495 500gcc gac ctt gtg gag cgc ggc tac gca acg ccg gag cag att gcg att 1651Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr Pro Glu Gln Ile Ala Ile
505 510 515cgt ggc gga tcc aac ggt ggt ttg ctg aca agt ggc gcg tta act cag 1699Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr Ser Gly Ala Leu Thr Gln
520 525 530tac cca gaa gca ttc ggt gcg gca gtt gtg cag gtg ccg ttg gct gat 1747Tyr Pro Glu Ala Phe Gly Ala Ala Val Val Gln Val Pro Leu Ala Asp
535 540 545atg ttg cgc tat cac acc tgg tca gcg ggt acc tcg tgg atg 1789Met Leu Arg Tyr His Thr Trp Ser Ala Gly Thr Ser Trp Met550 555 560taggtgtcgg caaccatggg aac 1812<210>120<211>563<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>120Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala Leu1 5 10 15Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser Val Glu Lys Leu Lys Ser
20 25 30Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp Thr
35 40 45Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr Asn
50 55 60Phe Trp Arg Asp Ala Gln His Pro Arg Gly Val Trp Arg Thr Thr Thr65 70 75 80Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu Trp Asp Val Leu Ile Asp
85 90 95Val Asp Ala Leu Ala Glu Asp Glu Gly Glu Asn Trp Val Trp Lys Gly
100 105 110Ala Val Val Arg Ser Pro Glu Phe Asp Arg Ala Leu Val Lys Phe Ser
115 120 125Arg Gly Gly Ala Asp Ala Thr Val Ile Arg Glu Phe Asp Leu Ala Thr
130 135 140Ala Ala Phe Val Asp Asp Ser Pro Phe Glu Leu Lys Glu Ala Lys Ser145 150 155 160Asp Val Thr Trp Val Asp Leu Asp Thr Leu Leu Val Gly Thr Asp Thr
165 170 175Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu Thr
180 185 190Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu Gly
195 200 205Ser Arg Gln Asp Val Ala Thr His Ala Trp Arg Asp Ser Thr Pro Gly
210 215 220Phe Glu Arg Thr Phe Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser Glu225 230 235 240Thr Ser Leu Glu Thr Glu Gly Gly Leu Val Lys Leu Asp Val Pro Thr
245 250 255Asp Cys Asp Val Ile Val Lys Lys Gln Trp Ile Phe Val Ser Pro Arg
260 265 270Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly Leu Gly Val Leu Leu Leu
275 280 285Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe Gln Pro Val Phe Thr Pro
290 295 300Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe Leu305 310 315 320Val Leu Thr Leu Leu Asn Asn Val Ser Thr Glu Ile Val Thr Val Pro
325 330 335Leu Asn Asp Pro Thr Thr Glu His Glu His Ile Asp Leu Pro Glu His
340 345 350Val Thr Ala His Val Val Ala Thr Ser Pro Leu Asp Gly Asp Glu Ile
355 360 365Trp Val Gln Ala Ala Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg Ala
370 375 380Glu Leu Pro Gly Ala Leu Glu Ala Val Lys Lys Ala Pro Leu Gln Phe385 390 395 400Glu Asn Ala Gly Gln Glu Thr Arg Gln His Trp Ala Thr Ser Ala Asp
405 410 415Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu Pro
420 425 430Gln Asn Thr Leu Val His Ala Tyr Gly Gly Phe Glu Val Ser Leu Thr
435 440 445Pro Ser His Ser Pro Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly Tyr
450 455 460Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro Glu465 470 475 480Trp His Ser Gln Ala Thr Lys Leu Asn Arg Met Lys Val Trp Glu Asp
485 490 495His Arg Ala Val Leu Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr Pro
500 505 510Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr Ser
515 520 525Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe Gly Ala Ala Val Val Gln
530 535 540Val Pro Leu Ala Asp Met Leu Arg Tyr His Thr Trp Ser Ala Gly Thr545 550 555 560Ser Trp Met<210>121<211>526<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(526)<223>RXA01914<400>121ggttttgagg aatggctagg cttgttaaaa gttagtttca atttgatgcc tcccccaacc 60aaagcggaga cacaacttca acgagaggac tcagctttca atg gcg aaa aat gcc 115
Met Ala Lys Asn Ala
l 5tac agc aca aca gca cca acc aag gtg tcc aag gat gcc act ctt cca 163Tyr Ser Thr Thr Ala Pro Thr Lys Val Ser Lys Asp Ala Thr Leu Pro
10 15 20gtt cgt gga acg gtc gct gaa ctc aag ctc gaa aag aag ttg cca aag 211Val Arg Gly Thr Val Ala Glu Leu Lys Leu Glu Lys Lys Leu Pro Lys
25 30 35aag att gat gcc atc atc gtc gcg att ttt gaa ggc gaa gat tcc atc 259Lys Ile Asp Ala Ile Ile Val Ala Ile Phe Glu Gly Glu Asp Ser Ile
40 45 50gaa ctc gcc ggc ggc gaa atc ctc gat ttc atc ttc agt acc gag cag 307Glu Leu Ala Gly Gly Glu Ile Leu Asp Phe Ile Phe Ser Thr Glu Gln
55 60 65cag gcc gac atc ctc act cag ctc gaa gct gtc ggc gca aag gcc acc 355Gln Ala Asp Ile Leu Thr Gln Leu Glu Ala Val Gly Ala Lys Ala Thr70 75 80 85gca aac agc atc acc cgc gtc cca ggc acc gac gtt gcg cct gtc att 403Ala Asn Ser Ile Thr Arg Val Pro Gly Thr Asp Val Ala Pro Val Ile
90 95 100gcg gtt ggt ttg ggc aag gct gat ttg ctt gac gac gag acc ctc cgc 451Ala Val Gly Leu Gly Lys Ala Asp Leu Leu Asp Asp Glu Thr Leu Arg
105 110 115cgc gct tcc ggc acg gcg gcc cgc tcc ctc ggt ggt ttt gaa aat gtc 499Arg Ala Ser Gly Thr Ala Ala Arg Ser Leu Gly Gly Phe Glu Asn Val
120 125 130gcc acc acc att ggc gat ttg gga ctt 526Ala Thr Thr Ile Gly Asp Leu Gly Leu
135 140<210>122<211>142<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>122Met Ala Lys Asn Ala Tyr Ser Thr Thr Ala Pro Thr Lys Val Ser Lys1 5 10 15Asp Ala Thr Leu Pro Val Arg Gly Thr Val Ala Glu Leu Lys Leu Glu
20 25 30Lys Lys Leu Pro Lys Lys Ile Asp Ala Ile Ile Val Ala Ile Phe Glu
35 40 45Gly Glu Asp Ser Ile Glu Leu Ala Gly Gly Glu Ile Leu Asp Phe Ile
50 55 60Phe Ser Thr Glu Gln Gln Ala Asp Ile Leu Thr Gln Leu Glu Ala Val65 70 75 80Gly Ala Lys Ala Thr Ala Asn Ser Ile Thr Arg Val Pro Gly Thr Asp
85 90 95Val Ala Pro Val Ile Ala Val Gly Leu Gly Lys Ala Asp Leu Leu Asp
100 105 110Asp Glu Thr Leu Arg Arg Ala Ser Gly Thr Ala Ala Arg Ser Leu Gly
115 120 125Gly Phe Glu Asn Val Ala Thr Thr Ile Gly Asp Leu Gly Leu
130 135 140<210>123<211>1497<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1474)<223>RXA02048<400>123gcgcggcgga taacaagcag accgcgtttg tggaatcgct gatctacacc tggatcgaaa 60agactttgga tcagcagggt agcatttaat acctatgatt atg cga agg ctg cgc 115
Met Arg Arg Leu Arg
1 5tcc acc ccg gtc cct ggt aca cgc gat tcc tac aca gga att gat ttc 163Ser Thr Pro Val Pro Gly Thr Arg Asp Ser Tyr Thr Gly Ile Asp Phe
10 15 20aac tta ggc ttc cac atc cga cgc tac gag ctt gat ctc acc tac cgc 211Ash Leu Gly Phe His Ile Arg Arg Tyr Glu Leu Asp Leu Thr Tyr Arg
25 30 35gta gca ccc aac ctg ctc atg ggc acc gca acg ctg cac atg gat aat 259Val Ala Pro Asn Leu Leu Met Gly Thr Ala Thr Leu His Met Asp Asn
40 45 50tac cgt gcg ctc gac gcg ctg acc ctg gac ctc ggc ggc agc ctg cgc 307Tyr Arg Ala Leu Asp Ala Leu Thr Leu Asp Leu Gly Gly Ser Leu Arg
55 60 65gtg gaa aaa gtc acc gcc aaa ggc acc gcc ggc acc cac atc caa gtc 355Val Glu Lys Val Thr Ala Lys Gly Thr Ala Gly Thr His Ile Gln Val70 75 80 85gcg cgc ttc cgc cac gcc ggc cgc aaa ctg cgc atc acc ttc cgc aac 403Ala Arg Phe Arg His Ala Gly Arg Lys Leu Arg Ile Thr Phe Arg Asn
90 95 100caa atc ccg gtt gac cag gaa ttt tca ctc acc atc cgc tac cgc ggc 451Gln Ile Pro Val Asp Gln Glu Phe Ser Leu Thr Ile Arg Tyr Arg Gly
105 110 115aac ccg cgc ccc ctg cgc agc gaa tgg ggc atg atc ggc tgg gaa gag 499Asn Pro Arg Pro Leu Arg Ser Glu Trp Gly Met Ile Gly Trp Glu Glu
120 125 130ctc gac aac ggc gcc ctc gtc gcc gcc cag cca aac ggc gcg ccg agc 547Leu Asp Asn Gly Ala Leu Val Ala Ala Gln Pro Asn Gly Ala Pro Ser
135 140 145tgg ttc ccc tgc gac gac acg ccc gac gag aag gcg ctt ttc gac gtc 595Trp Phe Pro Cys Asp Asp Thr Pro Asp Glu Lys Ala Leu Phe Asp Val150 155 160 165cac ttc cac acc gac aac gga tac gcc gcc att atc acc ggt gat tta 643His Phe His Thr Asp Asn Gly Tyr Ala Ala Ile Ile Thr Gly Asp Leu
170 175 180atc tca aaa cac gtc agt ggc agc atg acc acc tgg cac tac caa tcc 691Ile Ser Lys His Val Ser Gly Ser Met Thr Thr Trp His Tyr Gln Ser
185 190 195cgc gaa ccc atg gcc acc tac ctc gca gcc gtc cac gtc gga gaa tac 739Arg Glu Pro Met Ala Thr Tyr Leu Ala Ala Val His Val Gly Glu Tyr
200 205 210gac act gta tcc ctg ggc gtt tcg gaa tcg ggc gtt gtg gtg gag gcg 787Asp Thr Val Ser Leu Gly Val Ser Glu Ser Gly Val Val Val Glu Ala
215 220 225tat gtg cct gtg ggg gat gcg gcc ttg cgg gct cgg att ttg gag gac 835Tyr Val Pro Val Gly Asp Ala Ala Leu Arg Ala Arg Ile Leu Glu Asp230 235 240 245ttt gcc aaa caa gtc gac atg tta gac gcc tac gaa aaa ctc ttc ggc 883Phe Ala Lys Gln Val Asp Met Leu Asp Ala Tyr Glu Lys Leu Phe Gly
250 255 260ccc tac cca ttc cgc agc tac cgc gta gtc atc acc gaa gac gaa ctc 931Pro Tyr Pro Phe Arg Ser Tyr Arg Val Val Ile Thr Glu Asp Glu Leu
265 270 275gaa atc cca ctc gaa gcc caa ggc ctc tcc agc ttc gga gcc aac cac 979Glu Ile Pro Leu Glu Ala Gln Gly Leu Ser Ser Phe Gly Ala Asn His
280 285 290gcc acc ggc gaa gga acc tgg gaa cga ctc atc gcc cac gaa ctc tcc 1027Ala Thr Gly Glu Gly Thr Trp Glu Arg Leu Ile Ala His Glu Leu Ser
295 300 305cac cag tgg ttt ggc aac tca ctc ggc ctc gcc caa tgg aac gac atc 1075His Gln Trp Phe Gly Asn Ser Leu Gly Leu Ala Gln Trp Asn Asp Ile310 315 320 325tgg ctc aac gaa ggc ttc gcc tgt tac gcg gaa tgg ctc tgg ttt gag 1123Trp Leu Asn Glu Gly Phe Ala Cys Tyr Ala Glu Trp Leu Trp Phe Glu
330 335 340gca gct gga gtt aag tcg gct gcg gaa agt gcg ttg gaa ttc tat cga 1171Ala Ala Gly Val Lys Ser Ala Ala Glu Ser Ala Leu Glu Phe Tyr Arg
345 350 355ggc ctg gag gcg ctg ccg aag gat att ttg ctg gcc aac ccc ggc gcg 1219Gly Leu Glu Ala Leu Pro Lys Asp Ile Leu Leu Ala Asn Pro Gly Ala
360 365 370aag gat atg ttc gac gac cgc gtc tac aag cgc ggc gct ctg act gtc 1267Lys Asp Met Phe Asp Asp Arg Val Tyr Lys Arg Gly Ala Leu Thr Val
375 380 385cat gca ttg cgg gaa ttg ctt ggc gat gat gca ttc ttc aaa gct gtg 1315His Ala Leu Arg Glu Leu Leu Gly Asp Asp Ala Phe Phe Lys Ala Val390 395 400 405cgc tcc tac gtt gcc gaa ggc cga cac gga ctc gtt gaa ccc cgc gac 1363Arg Ser Tyr Val Ala Glu Gly Arg His Gly Leu Val Glu Pro Arg Asp
410 415 420ctg aaa cga cac ctc tac gca gtc tcc aca gac cac gca gct tta gat 1411Leu Lys Arg His Leu Tyr Ala Val Ser Thr Asp His Ala Ala Leu Asp
425 430 435gca gtg tgg cag tcc tgg ctt cgc gat ctg gag ttg ccg gag ttt cct 1459Ala Val Trp Gln Ser Trp Leu Arg Asp Leu Glu Leu Pro Glu Phe Pro
440 445 450tct ggt ggt ttg gac tagtgcgcta tctgacgctg gcc 1497Ser Gly Gly Leu Asp
455<210>124<211>458<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>124Met Arg Arg Leu Arg Ser Thr Pro Val Pro Gly Thr Arg Asp Ser Tyr1 5 10 15Thr Gly Ile Asp Phe Asn Leu Gly Phe His Ile Arg Arg Tyr Glu Leu
20 25 30Asp Leu Thr Tyr Arg Val Ala Pro Asn Leu Leu Met Gly Thr Ala Thr
35 40 45Leu His Met Asp Asn Tyr Arg Ala Leu Asp Ala Leu Thr Leu Asp Leu
50 55 60Gly Gly Ser Leu Arg Val Glu Lys Val Thr Ala Lys Gly Thr Ala Gly65 70 75 80Thr His Ile Gln Val Ala Arg Phe Arg His Ala Gly Arg Lys Leu Arg
85 90 95Ile Thr Phe Arg Asn Gln Ile Pro Val Asp Gln Glu Phe Ser Leu Thr
100 105 110Ile Arg Tyr Arg Gly Asn Pro Arg Pro Leu Arg Ser Glu Trp Gly Met
115 120 125Ile Gly Trp Glu Glu Leu Asp Asn Gly Ala Leu Val Ala Ala Gln Pro
130 135 140Asn Gly Ala Pro Ser Trp Phe Pro Cys Asp Asp Thr Pro Asp Glu Lys145 150 155 160Ala Leu Phe Asp Val His Phe His Thr Asp Asn Gly Tyr Ala Ala Ile
165 170 175Ile Thr Gly Asp Leu Ile Ser Lys His Val Ser Gly Ser Met Thr Thr
180 185 190Trp His Tyr Gln Ser Arg Glu Pro Met Ala Thr Tyr Leu Ala Ala Val
195 200 205His Val Gly Glu Tyr Asp Thr Val Ser Leu Gly Val Ser Glu Ser Gly
210 215 220Val Val Val Glu Ala Tyr Val Pro Val Gly Asp Ala Ala Leu Arg Ala225 230 235 240Arg Ile Leu Glu Asp Phe Ala Lys Gln Val Asp Met Leu Asp Ala Tyr
245 250 255Glu Lys Leu Phe Gly Pro Tyr Pro Phe Arg Ser Tyr Arg Val Val Ile
260 265 270Thr Glu Asp Glu Leu Glu Ile Pro Leu Glu Ala Gln Gly Leu Ser Ser
275 280 285Phe Gly Ala Asn His Ala Thr Gly Glu Gly Thr Trp Glu Arg Leu Ile
290 295 300Ala His Glu Leu Ser His Gln Trp Phe Gly Asn Ser Leu Gly Leu Ala305 310 315 320Gln Trp Asn Asp Ile Trp Leu Asn Glu Gly Phe Ala Cys Tyr Ala Glu
325 330 335Trp Leu Trp Phe Glu Ala Ala Gly Val Lys Ser Ala Ala Glu Ser Ala
340 345 350Leu Glu Phe Tyr Arg Gly Leu Glu Ala Leu Pro Lys Asp Ile Leu Leu
355 360 365Ala Asn Pro Gly Ala Lys Asp Met Phe Asp Asp Arg Val Tyr Lys Arg
370 375 380Gly Ala Leu Thr Val His Ala Leu Arg Glu Leu Leu Gly Asp Asp Ala385 390 395 400Phe Phe Lys Ala Val Arg Ser Tyr Val Ala Glu Gly Arg His Gly Leu
405 410 415Val Glu Pro Arg Asp Leu Lys Arg His Leu Tyr Ala Val Ser Thr Asp
420 425 430His Ala Ala Leu Asp Ala Val Trp Gln Ser Trp Leu Arg Asp Leu Glu
435 440 445Leu Pro Glu Phe Pro Ser Gly Gly Leu Asp
450 455<210>125<211>906<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(883)<223>RXN00621<400>125aaatgaatcc gggtttttca gtttcggggt gcaaatcaga atgtcgccaa tggcgaacac 60acgagcgtgc agaagatgtg cgtgactaag atcgggggct atg tct gaa cgc cta 115
Met Ser Glu Arg Leu
1 5aac gct ccg caa gca cca atc cat ccc atc acc cga acc cac cac ggt 163Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr Arg Thr His His Gly
10 15 20att gat ttc gta gac aac tat gaa tgg ctg agg gat aaa gaa tcc caa 211Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg Asp Lys Glu Ser Gln
25 30 35gaa acc ttg gac tac ctg gag gcg gag aat gcg ttc acc aag cag gag 259Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala Phe Thr Lys Gln Glu
40 45 50act gaa cag cta gcc aca ctg cgg gac aac atc tat gaa gag att aag 307Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile Tyr Glu Glu Ile Lys
55 60 65tca cgc gtt aaa gaa acc gac atg tcc atc cca gtg cgt gcc gga aag 355Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro Val Arg Ala Gly Lys70 75 80 85cac tgg tat tac tct cgc act gaa gaa ggc aag agc tac ggc tat tcc 403His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys Ser Tyr Gly Tyr Ser
90 95 100tgc cgc att cca gtg act gaa ggg tcg gat gca tgg acc cct cct gtt 451Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala Trp Thr Pro Pro Val
105 110 115atc cct gag ggt gag cca gcg cag ggt gaa acc atc atc atg gat gcc 499Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr Ile Ile Met Asp Ala
120 125 130aac gag ttg gca gaa ggc cac gaa ttc ttc tcc atg ggt gca tca tct 547Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser Met Gly Ala Ser Ser
135 140 145gtc acc acc tct ggc cgc tac ctt gcg tat tcc acc gat gtc acg ggc 595Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser Thr Asp Val Thr Gly150 155 160 165gaa gag cgc ttt acg ttg cgc atc aag gat cta gaa act ggc gag ctg 643Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu Glu Thr Gly Glu Leu
170 175 180ctt cct gat acc ctg act ggc att ttc tac ggt gct act tgg gtg ggg 691Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly Ala Thr Trp Val Gly
185 190 195gag gag tac ctc ttt tac cag cgc gtt gat gat gcg tgg cgt cca gat 739Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp Ala Trp Arg Pro Asp
200 205 210act gtg tgg cgc cac aag gtg ggt acc ccg gtt gaa gaa gac gtg ttg 787Thr Val Trp Arg His Lys Val Gly Thr Pro Val Glu Glu Asp Val Leu
215 220 225gtg tac cac gag cct gat gaa cgt tat tcc acc tgg gtg ggc acc act 835Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr Trp Val Gly Thr Thr230 235 240 245cgt tca gaa aaa gtt cat cct ttt tgg ttg cgc ctc caa gat cac ctc 883Arg Ser Glu Lys Val His Pro Phe Trp Leu Arg Leu Gln Asp His Leu
250 255 260tgaagtacgc gtgcttcctt tcg 906<210>126<211>261<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>126Met Ser Glu Arg Leu Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr1 5 10 15Arg Thr His His Gly Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg
20 25 30Asp Lys Glu Ser Gln Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala
35 40 45Phe Thr Lys Gln Glu Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile
50 55 60Tyr Glu Glu Ile Lys Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro65 70 75 80Val Arg Ala Gly Lys His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys
85 90 95Ser Tyr Gly Tyr Ser Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala
100 105 110Trp Thr Pro Pro Val Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr
115 120 125Ile Ile Met Asp Ala Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser
130 135 140Met Gly Ala Ser Ser Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser145 150 155 160Thr Asp Val Thr Gly Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu
165 170 175Glu Thr Gly Glu Leu Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly
180 185 190Ala Thr Trp Val Gly Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp
195 200 205Ala Trp Arg Pro Asp Thr Val Trp Arg His Lys Val Gly Thr Pro Val
210 215 220Glu Glu Asp Val Leu Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr225 230 235 240Trp Val Gly Thr Thr Arg Ser Glu Lys Val His Pro Phe Trp Leu Arg
245 250 255Leu Gln Asp His Leu
260<210>127<211>906<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(883)<223>FRXA00621<400>127aaatgaatcc gggtttttca gtttcggggt gcaaatcaga atgtcgccaa tggcgaacac 60acgagcgtgc agaagatgtg cgtgactaag atcgggggct atg tct gaa cgc cta 115
Met Ser Glu Arg Leu
1 5aac gct ccg caa gca cca atc cat ccc atc acc cga acc cac cac ggt 163Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr Arg Thr His His Gly
10 15 20att gat ttc gta gac aac tat gaa tgg ctg agg gat aaa gaa tcc caa 211Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg Asp Lys Glu Ser Gln
25 30 35gaa acc ttg gac tac ctg gag gcg gag aat gcg ttc acc aag cag gag 259Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala Phe Thr Lys Gln Glu
40 45 50act gaa cag cta gcc aca ctg cgg gac aac atc tat gaa gag att aag 307Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile Tyr Glu Glu Ile Lys
55 60 65tca cgc gtt aaa gaa acc gac atg tcc atc cca gtg cgt gcc gga aag 355Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro Val Arg Ala Gly Lys70 75 80 85cac tgg tat tac tct cgc act gaa gaa ggc aag agc tac ggc tat tcc 403His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys Ser Tyr Gly Tyr Ser
90 95 100tgc cgc att cca gtg act gaa ggg tcg gat gca tgg acc cct cct gtt 451Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala Trp Thr Pro Pro Val
105 110 115atc cct gag ggt gag cca gcg cag ggt gaa acc atc atc atg gat gcc 499Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr Ile Ile Met Asp Ala
120 125 130aac gag ttg gca gaa ggc cac gaa ttc ttc tcc atg ggt gca tca tct 547Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser Met Gly Ala Ser Ser
135 140 145gtc acc acc tct ggc cgc tac ctt gcg tat tcc acc gat gtc acg ggc 595Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser Thr Asp Val Thr Gly150 155 160 165gaa gag cgc ttt acg ttg cgc atc aag gat cta gaa act ggc gag ctg 643Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu Glu Thr Gly Glu Leu
170 175 180ctt cct gat acc ctg act ggc att ttc tac ggt gct act tgg gtg ggg 691Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly Ala Thr Trp Val Gly
185 190 195gag gag tac ctc ttt tac cag cgc gtt gat gat gcg tgg cgt cca gat 739Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp Ala Trp Arg Pro Asp
200 205 210act gtg tgg cgc cac aag gtg ggt acc ccg gtt gaa gaa gac gtg ttg 787Thr Val Trp Arg His Lys Val Gly Thr Pro Val Glu Glu Asp Val Leu
215 220 225gtg tac cac gag cct gat gaa cgt tat tcc acc tgg gtg ggc acc act 835Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr Trp Val Gly Thr Thr230 235 240 245cgt tca gaa aaa gtt cat cct ttt tgg ttg cgc ctc caa gat cac ctc 883Arg Ser Glu Lys Val His Pro Phe Trp Leu Arg Leu Gln Asp His Leu
250 255 260tgaagtacgc gtgcttcctt tcg 906<210>128<211>261<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>128Met Ser Glu Arg Leu Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr1 5 10 15Arg Thr His His Gly Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg
20 25 30Asp Lys Glu Ser Gln Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala
35 40 45Phe Thr Lys Gln Glu Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile
50 55 60Tyr Glu Glu Ile Lys Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro65 70 75 80Val Arg Ala Gly Lys His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys
85 90 95Ser Tyr Gly Tyr Ser Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala
100 105 110Trp Thr Pro Pro Val Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr
115 120 125Ile Ile Met Asp Ala Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser
130 135 140Met Gly Ala Ser Ser Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser145 150 155 160Thr Asp Val Thr Gly Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu
165 170 175Glu Thr Gly Glu Leu Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly
180 185 190Ala Thr Trp Val Gly Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp
195 200 205Ala Trp Arg Pro Asp Thr Val Trp Arg His Lys Val Gly Thr Pro Val
210 215 220Glu Glu Asp Val Leu Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr225 230 235 240Trp Val Gly Thr Thr Arg Ser Glu Lys Val His Pro Phe Trp Leu Arg
245 250 255Leu Gln Asp His Leu
260<210>129<211>1539<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1516)<223>RXN00622<400>129ttttaccagc gcgttgatga tgcgtggcgt ccagatactg tgtggcgcca caaggtgggt 60accccggttg aagaagacgt gttggtgtac cacgagcctg atg aac gtt att cca 115
Met Asn Val Ile Pro
1 5cct ggg tgg gca cca ctc gtt cag aaa aag ttc atc ctt ttt ggt tgc 163Pro Gly Trp Ala Pro Leu Val Gln Lys Lys Phe Ile Leu Phe Gly Cys
10 15 20gcc tcc aag atc acc tct gaa gta cgc gtg ctt cct ttc gac cag cca 211Ala Ser Lys Ile Thr Ser Glu Val Arg Val Leu Pro Phe Asp Gln Pro
25 30 35gag ggc acc cct gag gtg ctg att ccg cgc gcg gag ggt gtg gaa tac 259Glu Gly Thr Pro Glu Val Leu Ile Pro Arg Ala Glu Gly Val Glu Tyr
40 45 50gac gtc gat cat gca gtc gta gac ggc tcc gat att tgg ttg gtc aca 307Asp Val Asp His Ala Val Val Asp Gly Ser Asp Ile Trp Leu Val Thr
55 60 65cac aac gcc gag ggc ccg aac ttt tcg gtg ggg tgg gct ggc gtc gac 355His Asn Ala Glu Gly Pro Asn Phe Ser Val Gly Trp Ala Gly Val Asp70 75 80 85aag ctc aat tct ttg gac gcg ctg gcg cca ctc gtc gcg cac aag gat 403Lys Leu Asn Ser Leu Asp Ala Leu Ala Pro Leu Val Ala His Lys Asp
90 95 100gac gtg cgc att gag ggt gtc gat acc tac cgc gat ttc atc atc ctg 451Asp Val Arg Ile Glu Gly Val Asp Thr Tyr Arg Asp Phe Ile Ile Leu
105 110 115ggc tac agg tcc ggc gcg atc ggc cag gtc gcg atc atg aag ctt atc 499Gly Tyr Arg Ser Gly Ala Ile Gly Gln Val Ala Ile Met Lys Leu Ile
120 125 130gac gga acc ttc ggc gat ttc caa cag ctg gaa ttt gac gag gaa atc 547Asp Gly Thr Phe Gly Asp Phe Gln Gln Leu Glu Phe Asp Glu Glu Ile
135 140 145tac acc gtc gca tcg ggc gga aac cca gaa tgg gac gcc ccc gtc att 595Tyr Thr Val Ala Ser Gly Gly Asn Pro Glu Trp Asp Ala Pro Val Ile150 155 160 165cgc ctt tct tac gga tca ttc acc acc ccg gcg cag ctg ttt aac tac 643Arg Leu Ser Tyr Gly Ser Phe Thr Thr Pro Ala Gln Leu Phe Asn Tyr
170 175 180tgg att gaa tcc ggc gaa cgc acg ctg ctg aag cag cag gaa gtg ctc 691Trp Ile Glu Ser Gly Glu Arg Thr Leu Leu Lys Gln Gln Glu Val Leu
185 190 195ggc gga tac aag ccg tca gac tat gtg gcc tcc cga ttg tgg gtc act 739Gly Gly Tyr Lys Pro Ser Asp Tyr Val Ala Ser Arg Leu Trp Val Thr
200 205 210gcg aaa gat ggc gcg cag att cca gtg tcc ttg gtg cac cgc acc gac 787Ala Lys Asp Gly Ala Gln Ile Pro Val Ser Leu Val His Arg Thr Asp
215 220 225ctg gat gta tcc aag ccc aac ccc acg ttg ctc tac ggc tat ggt tcc 835Leu Asp Val Ser Lys Pro Asn Pro Thr Leu Leu Tyr Gly Tyr Gly Ser230 235 240 245tac gaa tca tcc att gat cca ggc ttc tct atc gcg cgt ttg tca ctg 883Tyr Glu Ser Ser Ile Asp Pro Gly Phe Ser Ile Ala Arg Leu Ser Leu
250 255 260atg gat cgt ggc atg att ttt gcg att gcc cac gtt cgt ggc ggt ggc 931Met Asp Arg Gly Met Ile Phe Ala Ile Ala His Val Arg Gly Gly Gly
265 270 275gaa atg ggt cgt ggc tgg tac gac aac ggc aaa acc acc acg aag aaa 979Glu Met Gly Arg Gly Trp Tyr Asp Asn Gly Lys Thr Thr Thr Lys Lys
280 285 290aac acc ttc acc gac ttc att gat gtt gcc gac gcc ctc atc gag cag 1027Asn Thr Phe Thr Asp Phe Ile Asp Val Ala Asp Ala Leu Ile Glu Gln
295 300 305aag att tct gcc cct gaa atg ctg gtt gca gaa ggc ggc tca gct ggt 1075Lys Ile Ser Ala Pro Glu Mer Leu Val Ala Glu Gly Gly Ser Ala Gly310 315 320 325ggc atg ctc atg ggc gcc att gcc aac atg gcc ggt gac cgc ttc aag 1123Gly Met Leu Met Gly Ala Ile Ala Asn Met Ala Gly Asp Arg Phe Lys
330 335 340gcg atc gaa gcc aac gtg cca ttc gtc gat ccg ctg acc tct atg ctc 1171Ala Ile Glu Ala Asn Val Pro Phe Val Asp Pro Leu Thr Ser Met Leu
345 350 355atg ccg gaa ctg cca ctg acg gtt atc gaa tgg gat gag tgg ggc gat 1219Met Pro Glu Leu Pro Leu Thr Val Ile Glu Trp Asp Glu Trp Gly Asp
360 365 370cca ctc cac gat aag gac gtc tat gaa tac atg gcg tcg tat gcc cca 1267Pro Leu His Asp Lys Asp Val Tyr Glu Tyr Met Ala Ser Tyr Ala Pro
375 380 385tat gaa aac atc gag gca aag aac tac ccc aat atc ttg gcc gta aca 1315Tyr Glu Asn Ile Glu Ala Lys Asn Tyr Pro Asn Ile Leu Ala Val Thr390 395 400 405tcg ctc aac gac acc cga gtg ttg tac gtc gaa cca gcc aaa tgg gta 1363Ser Leu Asn Asp Thr Arg Val Leu Tyr Val Glu Pro Ala Lys Trp Val
410 415 420gcg cag ctt cgg gcg act gca acc ggt gga gaa ttc ctt ctg aaa act 1411Ala Gln Leu Arg Ala Thr Ala Thr Gly Gly Glu Phe Leu Leu Lys Thr
425 430 435gaa atg gtt gcc gga cac ggc ggt gtg tca gga cgc tac gaa aag tgg 1459Glu Met Val Ala Gly His Gly Gly Val Ser Gly Arg Tyr Glu Lys Trp
440 445 450cgt gag act gca ttt gag tac ggc tgg ttg atc aac caa gca acc ggt 1507Arg Glu Thr Ala Phe Glu Tyr Gly Trp Leu Ile Asn Gln Ala Thr Gly
455 460 465gtg acc gaa taaaacttgt tcgactagcg aac 1539Val Thr Glu470<210>130<211>472<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>130Met Asn Val Ile Pro Pro Gly Trp Ala Pro Leu Val Gln Lys Lys Phe1 5 10 15Ile Leu Phe Gly Cys Ala Ser Lys Ile Thr Ser Glu Val Arg Val Leu
20 25 30Pro Phe Asp Gln Pro Glu Gly Thr Pro Glu Val Leu Ile Pro Arg Ala
35 40 45Glu Gly Val Glu Tyr Asp Val Asp His Ala Val Val Asp Gly Ser Asp
50 55 60Ile Trp Leu Val Thr His Asn Ala Glu Gly Pro Asn Phe Ser Val Gly65 70 75 80Trp Ala Gly Val Asp Lys Leu Asn Ser Leu Asp Ala Leu Ala Pro Leu
85 90 95Val Ala His Lys Asp Asp Val Arg Ile Glu Gly Val Asp Thr Tyr Arg
100 105 110Asp Phe Ile Ile Leu Gly Tyr Arg Ser Gly Ala Ile Gly Gln Val Ala
115 120 125Ile Met Lys Leu Ile Asp Gly Thr Phe Gly Asp Phe Gln Gln Leu Glu
130 135 140Phe Asp Glu Glu Ile Tyr Thr Val Ala Ser Gly Gly Asn Pro Glu Trp145 150 155 160Asp Ala Pro Val Ile Arg Leu Ser Tyr Gly Ser Phe Thr Thr Pro Ala
165 170 175Gln Leu Phe Asn Tyr Trp Ile Glu Ser Gly Glu Arg Thr Leu Leu Lys
180 185 190Gln Gln Glu Val Leu Gly Gly Tyr Lys Pro Ser Asp Tyr Val Ala Ser
195 200 205Arg Leu Trp Val Thr Ala Lys Asp Gly Ala Gln Ile Pro Val Ser Leu
210 215 220Val His Arg Thr Asp Leu Asp Val Ser Lys Pro Asn Pro Thr Leu Leu225 230 235 240Tyr Gly Tyr Gly Ser Tyr Glu Ser Ser Ile Asp Pro Gly Phe Ser Ile
245 250 255Ala Arg Leu Ser Leu Met Asp Arg Gly Met Ile Phe Ala Ile Ala His
260 265 270Val Arg Gly Gly Gly Glu Met Gly Arg Gly Trp Tyr Asp Asn Gly Lys
275 280 285Thr Thr Thr Lys Lys Asn Thr Phe Thr Asp Phe Ile Asp Val Ala Asp
290 295 300Ala Leu Ile Glu Gln Lys Ile Ser Ala Pro Glu Met Leu Val Ala Glu305 310 315 320Gly Gly Ser Ala Gly Gly Met Leu Met Gly Ala Ile Ala Asn Met Ala
325 330 335Gly Asp Arg Phe Lys Ala Ile Glu Ala Asn Val Pro Phe Val Asp Pro
340 345 350Leu Thr Ser Met Leu Met Pro Glu Leu Pro Leu Thr Val Ile Glu Trp
355 360 365Asp Glu Trp Gly Asp Pro Leu His Asp Lys Asp Val Tyr Glu Tyr Met
370 375 380Ala Ser Tyr Ala Pro Tyr Glu Asn Ile Glu Ala Lys Asn Tyr Pro Asn385 390 395 400Ile Leu Ala Val Thr Ser Leu Asn Asp Thr Arg Val Leu Tyr Val Glu
405 410 415Pro Ala Lys Trp Val Ala Gln Leu Arg Ala Thr Ala Thr Gly Gly Glu
420 425 430Phe Leu Leu Lys Thr Glu Met Val Ala Gly His Gly Gly Val Ser Gly
435 440 445Arg Tyr Glu Lys Trp Arg Glu Thr Ala Phe Glu Tyr Gly Trp Leu Ile
450 455 460Asn Gln Ala Thr Gly Val Thr Glu465 470<210>131<211>1539<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1516)<223>FRXA00622<400>131ttttaccagc gcgttgatga tgcgtggcgt ccagatactg tgtggcgcca caaggtgggt 60accccggttg aagaagacgt gttggtgtac cacgagcctg atg aac gtt att cca 115
Met Asn Val Ile Pro
1 5cct ggg tgg gca cca ctc gtt cag aaa aag ttc atc ctt ttt ggt tgc 163Pro Gly Trp Ala Pro Leu Val Gln Lys Lys Phe Ile Leu Phe Gly Cys
10 15 20gcc tcc aag atc acc tct gaa gta cgc gtg ctt cct ttc gac cag cca 211Ala Ser Lys Ile Thr Ser Glu Val Arg Val Leu Pro Phe Asp Gln Pro
25 30 35gag ggc acc cct gag gtg ctg att ccg cgc gcg gag ggt gtg gaa tac 259Glu Gly Thr Pro Glu Val Leu Ile Pro Arg Ala Glu Gly Val Glu Tyr
40 45 50gac gtc gat cat gca gtc gta gac ggc tcc gat att tgg ttg gtc aca 307Asp Val Asp His Ala Val Val Asp Gly Ser Asp Ile Trp Leu Val Thr
55 60 65cac aac gcc gag ggc ccg aac ttt tcg gtg ggg tgg gct ggc gtc gac 355His Asn Ala Glu Gly Pro Asn Phe Ser Val Gly Trp Ala Gly Val Asp70 75 80 85aag ctc aat tct ttg gac gcg ctg gcg cca ctc gtc gcg cac aag gat 403Lys Leu Asn Ser Leu Asp Ala Leu Ala Pro Leu Val Ala His Lys Asp
90 95 100gac gtg cgc att gag ggt gtc gat acc tac cgc gat ttc atc atc ctg 451Asp Val Arg Ile Glu Gly Val Asp Thr Tyr Arg Asp Phe Ile Ile Leu
105 110 115ggc tac agg tcc ggc gcg atc ggc cag gtc gcg atc atg aag ctt atc 499Gly Tyr Arg Ser Gly Ala Ile Gly Gln Val Ala Ile Met Lys Leu Ile
120 125 130gac gga acc ttc ggc gat ttc caa cag ctg gaa ttt gac gag gaa atc 547Asp Gly Thr Phe Gly Asp Phe Gln Gln Leu Glu Phe Asp Glu Glu Ile
135 140 145tac acc gtc gca tcg ggc gga aac cca gaa tgg gac gcc ccc gtc att 595Tyr Thr Val Ala Ser Gly Gly Asn Pro Glu Trp Asp Ala Pro Val Ile150 155 160 165cgc ctt tct tac gga tca ttc acc acc ccg gcg cag ctg ttt aac tac 643Arg Leu Ser Tyr Gly Ser Phe Thr Thr Pro Ala Gln Leu Phe Asn Tyr
170 175 180tgg att gaa tcc ggc gaa cgc acg ctg ctg aag cag cag gaa gtg ctc 691Trp Ile Glu Ser Gly Glu Arg Thr Leu Leu Lys Gln Gln Glu Val Leu
185 190 195ggc gga tac aag ccg tca gac tat gtg gcc tcc cga ttg tgg gtc act 739Gly Gly Tyr Lys Pro Ser Asp Tyr Val Ala Ser Arg Leu Trp Val Thr
200 205 210gcg aaa gat ggc gcg cag att cca gtg tcc ttg gtg cac cgc acc gac 787Ala Lys Asp Gly Ala Gln Ile Pro Val Ser Leu Val His Arg Thr Asp
215 220 225ctg gat gta tcc aag ccc aac ccc acg ttg ctc tac ggc tat ggt tcc 835Leu Asp Val Ser Lys Pro Asn Pro Thr Leu Leu Tyr Gly Tyr Gly Ser230 235 240 245tac gaa tca tcc att gat cca ggc ttc tct atc gcg cgt ttg tca ctg 883Tyr Glu Ser Ser Ile Asp Pro Gly Phe Ser Ile Ala Arg Leu Ser Leu
250 255 260atg gat cgt ggc atg att ttt gcg att gcc cac gtt cgt ggc ggt ggc 931Met Asp Arg Gly Met Ile Phe Ala Ile Ala His Val Arg Gly Gly Gly
265 270 275gaa atg ggt cgt ggc tgg tac gac aac ggc aaa acc acc acg aag aaa 979Glu Met Gly Arg Gly Trp Tyr Asp Asn Gly Lys Thr Thr Thr Lys Lys
280 285 290aac acc ttc acc gac ttc att gat gtt gcc gac gcc ctc atc gag cag 1027Asn Thr Phe Thr Asp Phe Ile Asp Val Ala Asp Ala Leu Ile Glu Gln
295 300 305aag att tct gcc cct gaa atg ctg gtt gca gaa ggc ggc tca gct ggt 1075Lys Ile Ser Ala Pro Glu Met Leu Val Ala Glu Gly Gly Ser Ala Gly310 315 320 325ggc atg ctc atg ggc gcc att gcc aac atg gcc ggt gac cgc ttc aag 1123Gly Met Leu Met Gly Ala Ile Ala Asn Met Ala Gly Asp Arg Phe Lys
330 335 340gcg atc gaa gcc aac gtg cca ttc gtc gat ccg ctg acc tct atg ctc 1171Ala Ile Glu Ala Asn Val Pro Phe Val Asp Pro Leu Thr Ser Met Leu
345 350 355atg ccg gaa ctg cca ctg acg gtt atc gaa tgg gat gag tgg ggc gat 1219Met Pro Glu Leu Pro Leu Thr Val Ile Glu Trp Asp Glu Trp Gly Asp
360 365 370cca ctc cac gat aag gac gtc tat gaa tac atg gcg tcg tat gcc cca 1267Pro Leu His Asp Lys Asp Val Tyr Glu Tyr Met Ala Ser Tyr Ala Pro
375 380 385tat gaa aac atc gag gca aag aac tac ccc aat atc ttg gcc gta aca 1315Tyr Glu Asn Ile Glu Ala Lys Asn Tyr Pro Asn Ile Leu Ala Val Thr390 395 400 405tcg ctc aac gac acc cga gtg ttg tac gtc gaa cca gcc aaa tgg gta 1363Ser Leu Asn Asp Thr Arg Val Leu Tyr Val Glu Pro Ala Lys Trp Val
410 415 420gcg cag ctt cgg gcg act gca acc ggt gga gaa ttc ctt ctg aaa act 1411Ala Gln Leu Arg Ala Thr Ala Thr Gly Gly Glu Phe Leu Leu Lys Thr
425 430 435gaa atg gtt gcc gga cac ggc ggt gtg tca gga cgc tac gaa aag tgg 1459Glu Met Val Ala Gly His Gly Gly Val Ser Gly Arg Tyr Glu Lys Trp
440 445 450cgt gag act gca ttt gag tac ggc tgg ttg atc aac caa gca acc ggt 1507Arg Glu Thr Ala Phe Glu Tyr Gly Trp Leu Ile Asn Gln Ala Thr Gly
455 460 465gtg acc gaa taaaacttgt tcgactagcg aac 1539Val Thr Glu470<210>132<211>472<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>132Met Asn Val Ile Pro Pro Gly Trp Ala Pro Leu Val Gln Lys Lys Phe1 5 10 15Ile Leu Phe Gly Cys Ala Ser Lys Ile Thr Ser Glu Val Arg Val Leu
20 25 30Pro Phe Asp Gln Pro Glu Gly Thr Pro Glu Val Leu Ile Pro Arg Ala
35 40 45Glu Gly Val Glu Tyr Asp Val Asp His Ala Val Val Asp Gly Ser Asp
50 55 60Ile Trp Leu Val Thr His Asn Ala Glu Gly Pro Asn Phe Ser Val Gly65 70 75 80Trp Ala Gly Val Asp Lys Leu Asn Ser Leu Asp Ala Leu Ala Pro Leu
85 90 95Val Ala His Lys Asp Asp Val Arg Ile Glu Gly Val Asp Thr Tyr Arg
100 105 110Asp Phe Ile Ile Leu Gly Tyr Arg Ser Gly Ala Ile Gly Gln Val Ala
115 120 125Ile Met Lys Leu Ile Asp Gly Thr Phe Gly Asp Phe Gln Gln Leu Glu
130 135 140Phe Asp Glu Glu Ile Tyr Thr Val Ala Ser Gly Gly Asn Pro Glu Trp145 150 155 160Asp Ala Pro Val Ile Arg Leu Ser Tyr Gly Ser Phe Thr Thr Pro Ala
165 170 175Gln Leu Phe Asn Tyr Trp Ile Glu Ser Gly Glu Arg Thr Leu Leu Lys
180 185 190Gln Gln Glu Val Leu Gly Gly Tyr Lys Pro Ser Asp Tyr Val Ala Ser
195 200 205Arg Leu Trp Val Thr Ala Lys Asp Gly Ala Gln Ile Pro Val Ser Leu
210 215 220Val His Arg Thr Asp Leu Asp Val Ser Lys Pro Asn Pro Thr Leu Leu225 230 235 240Tyr Gly Tyr Gly Ser Tyr Glu Ser Ser Ile Asp Pro Gly Phe Ser Ile
245 250 255Ala Arg Leu Ser Leu Met Asp Arg Gly Met Ile Phe Ala Ile Ala His
260 265 270Val Arg Gly Gly Gly Glu Met Gly Arg Gly Trp Tyr Asp Asn Gly Lys
275 280 285Thr Thr Thr Lys Lys Asn Thr Phe Thr Asp Phe Ile Asp Val Ala Asp
290 295 300Ala Leu Ile Glu Gln Lys Ile Ser Ala Pro Glu Met Leu Val Ala Glu305 310 315 320Gly Gly Ser Ala Gly Gly Met Leu Met Gly Ala Ile Ala Asn Met Ala
325 330 335Gly Asp Arg Phe Lys Ala Ile Glu Ala Asn Val Pro Phe Val Asp Pro
340 345 350Leu Thr Ser Met Leu Met Pro Glu Leu Pro Leu Thr Val Ile Glu Trp
355 360 365Asp Glu Trp Gly Asp Pro Leu His Asp Lys Asp Val Tyr Glu Tyr Met
370 375 380Ala Ser Tyr Ala Pro Tyr Glu Asn Ile Glu Ala Lys Asn Tyr Pro Asn385 390 395 400Ile Leu Ala Val Thr Ser Leu Asn Asp Thr Arg Val Leu Tyr Val Glu
405 410 415Pro Ala Lys Trp Val Ala Gln Leu Arg Ala Thr Ala Thr Gly Gly Glu
420 425 430Phe Leu Leu Lys Thr Glu Met Val Ala Gly His Gly Gly Val Ser Gly
435 440 445Arg Tyr Glu Lys Trp Arg Glu Thr Ala Phe Glu Tyr Gly Trp Leu Ile
450 455 460Asn Gln Ala Thr Gly Val Thr Glu465 470<210>133<211>1629<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1606)<223>RXN00982<400>133gaaaacaaac gtccttgaag ccgtaatgcc ccgttcgaca ataaaaaggg tagtagcagt 60tcttgccgcc tcgactgcgc ttagcccctt tttggtatca atg ccc act gca gca 115
Met Pro Thr Ala Ala
1 5gcg caa gaa aac atc cgc tgg gaa gaa tgc cca cct cag gta gat att 163Ala Gln Glu Asn Ile Arg Trp Glu Glu Cys Pro Pro Gln Val Asp Ile
10 15 20gcc tcc gct caa tgt ggc agc atc gac gtg ccc atg cac tat tct gat 211Ala Ser Ala Gln Cys Gly Ser Ile Asp Val Pro Met His Tyr Ser Asp
25 30 35ccc tca ctt ggc gat atc agc gtg ggc ttt gtc aag gtc cct gcc caa 259Pro Ser Leu Gly Asp Ile Ser Val Gly Phe Val Lys Val Pro Ala Gln
40 45 50ggc gaa aag cac ggc acc atc ttc ggt aac tcc ggt ggc cct ggt ggc 307Gly Glu Lys His Gly Thr Ile Phe Gly Asn Ser Gly Gly Pro Gly Gly
55 60 65gat gcc tat agc ttc ttc ggc agc caa tcc atg aac tgg cca gaa gcc 355Asp Ala Tyr Ser Phe Phe Gly Ser Gln Ser Met Asn Trp Pro Glu Ala70 75 80 85atg tac caa aac tac gac ctc gtt gca gtg cag cct cgc gga atg gtc 403Met Tyr Gln Asn Tyr Asp Leu Val Ala Val Gln Pro Arg Gly Met Val
90 95 100ggc tcc aca ccg gtt aac tgc gac aac atc gca cca gga tac gat ttc 451Gly Ser Thr Pro Val Asn Cys Asp Asn Ile Ala Pro Gly Tyr Asp Phe
105 110 115ctc tcg ctg ctc acc cgc gaa ggc gct ttc gtt aaa gaa tcc tgc gag 499Leu Ser Leu Leu Thr Arg Glu Gly Ala Phe Val Lys Glu Ser Cys Glu
120 125 130atc ggc acc ccc ggc tac acc tcc agc ctg acc acc gac aac acc gcc 547Ile Gly Thr Pro Gly Tyr Thr Ser Ser Leu Thr Thr Asp Asn Thr Ala
135 140 145aac gac tgg gag cgc gtc cgc caa gca ctt ggc gat gac aag atc tcc 595Asn Asp Trp Glu Arg Val Arg Gln Ala Leu Gly Asp Asp Lys Ile Ser150 155 160 165atc ttc gga ctg tcc tac gga acc tac ctc gga tcg gtc tac gcc acc 643Ile Phe Gly Leu Ser Tyr Gly Thr Tyr Leu Gly Ser Val Tyr Ala Thr
170 175 180cgc tac cca cag cac acc gac aag gtt gtc ctc gat tcc gca atg gcg 691Arg Tyr Pro Gln His Thr Asp Lys Val Val Leu Asp Ser Ala Met Ala
185 190 195ccc agc ctg gca tgg aac ggc atc atg gcc tcc caa gaa cag ggc tac 739Pro Ser Leu Ala Trp Asn Gly Ile Met Ala Ser Gln Glu Gln Gly Tyr
200 205 210aaa aac tcc ctc aac gac ttc ttc acc tgg gtt gca gaa aac aac gac 787Lys Asn Ser Leu Asn Asp Phe Phe Thr Trp Val Ala Glu Asn Asn Asp
215 220 225acg tat ggc ctc ggc act acc cca cta gcc gtg tac caa aac tgg tca 835Thr Tyr Gly Leu Gly Thr Thr Pro Leu Ala Val Tyr Gln Asn Trp Ser230 235 240 245aac aag atc gtc gcc gaa acc gga acc aac cca acc gtt gct cca cca 883Asn Lys Ile Val Ala Glu Thr Gly Thr Asn Pro Thr Val Ala Pro Pro
250 255 260cca gca caa gtt ggc gat gtc cca cca gca ttc gca tgg gcc ggc caa 931Pro Ala Gln Val Gly Asp Val Pro Pro Ala Phe Ala Trp Ala Gly Gln
265 270 275gca ggc gca gac atg atg acc gcc acc aac cca acc tcc gtg caa ctc 979Ala Gly Ala Asp Met Met Thr Ala Thr Asn Pro Thr Ser Val Gln Leu
280 285 290cag ggc ctt gcc acc cag ctc cta aac cct gga tcc aac cag tca ctg 1027Gln Gly Leu Ala Thr Gln Leu Leu Asn Pro Gly Ser Asn Gln Ser Leu
295 300 305agc cct ctg ctc aac gtc acc cgc gcc tac att cca cag cca tca acc 1075Ser Pro Leu Leu Asn Val Thr Arg Ala Tyr Ile Pro Gln Pro Ser Thr310 315 320 325tgg ccc atg ctc gca ggc gcc atc tca ggg caa aca ccc atc cct gac 1123Trp Pro Met Leu Ala Gly Ala Ile Ser Gly Gln Thr Pro Ile Pro Asp
330 335 340gta act gac acc ggc gac gac cca tac gtc atc gaa agc atc aac gcc 1171Val Thr Asp Thr Gly Asp Asp Pro Tyr Val Ile Glu Ser Ile Asn Ala
345 350 355agc gtc aac atg cag cgc atg gtc atg tgc aac gaa aac acc gtc gca 1219Ser Val Asn Met Gln Arg Met Val Met Cys Asn Glu Asn Thr Val Ala
360 365 370cca gac cca gta gca atg gca cgc atg gcc tgg aca agc atg gtc acc 1267Pro Asp Pro Val Ala Met Ala Arg Met Ala Trp Thr Ser Met Val Thr
375 380 385ggc gac gtc ttt gac att tac tcc gtt aaa ttc agc tcc gga caa gcc 1315Gly Asp Val Phe Asp Ile Tyr Ser Val Lys Phe Ser Ser Gly Gln Ala390 395 400 405tgc tcc ggc atc acc cca aca agc ggc cgc cag cca acc gac gga tct 1363Cys Ser Gly Ile Thr Pro Thr Ser Gly Arg Gln Pro Thr Asp Gly Ser
410 415 420caa cta gca gtc caa cca cta ctc ctc cag gga acc agc gac cca caa 1411Gln Leu Ala Val Gln Pro Leu Leu Leu Gln Gly Thr Ser Asp Pro Gln
425 430 435acc cca tac tgg acc cac aac gag ctt gcc gac gcc atg aac gcc cac 1459Thr Pro Tyr Trp Thr His Asn Glu Leu Ala Asp Ala Met Asn Ala His
440 445 450gtg gtc acc gtc aac gga cca gga cac ggc caa tcc atc ggc ggc acc 1507Val Val Thr Val Asn Gly Pro Gly His Gly Gln Ser Ile Gly Gly Thr
455 460 465aac caa gca atc aac gac att gtt gtg gac tac ctc cgc acc gga cac 1555Asn Gln Ala Ile Asn Asp Ile Val Val Asp Tyr Leu Arg Thr Gly His470 475 480 485acc gac gcc acc tgg gtc gaa ggc aac aca ccc acc cca att acg gct 1603Thr Asp Ala Thr Trp Val Glu Gly Asn Thr Pro Thr Pro Ile Thr Ala
490 495 500ggc taattgcttt ccacttagta gat 1629Gly<210>134<211>502<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>134Met Pro Thr Ala Ala Ala Gln Glu Asn Ile Arg Trp Glu Glu Cys Pro1 5 10 15Pro Gln Val Asp Ile Ala Ser Ala Gln Cys Gly Ser Ile Asp Val Pro
20 25 30Met His Tyr Ser Asp Pro Ser Leu Gly Asp Ile Ser Val Gly Phe Val
35 40 45Lys Val Pro Ala Gln Gly Glu Lys His Gly Thr Ile Phe Gly Asn Ser
50 55 60Gly Gly Pro Gly Gly Asp Ala Tyr Ser Phe Phe Gly Ser Gln Ser Met65 70 75 80Asn Trp Pro Glu Ala Met Tyr Gln Asn Tyr Asp Leu Val Ala Val Gln
85 90 95Pro Arg Gly Met Val Gly Ser Thr Pro Val Asn Cys Asp Asn Ile Ala
100 105 110Pro Gly Tyr Asp Phe Leu Ser Leu Leu Thr Arg Glu Gly Ala Phe Val
115 120 125Lys Glu Ser Cys Glu Ile Gly Thr Pro Gly Tyr Thr Ser Ser Leu Thr
130 135 140Thr Asp Asn Thr Ala Asn Asp Trp Glu Arg Val Arg Gln Ala Leu Gly145 150 155 160Asp Asp Lys Ile Ser Ile Phe Gly Leu Ser Tyr Gly Thr Tyr Leu Gly
165 170 175Ser Val Tyr Ala Thr Arg Tyr Pro Gln His Thr Asp Lys Val Val Leu
180 185 190Asp Ser Ala Met Ala Pro Ser Leu Ala Trp Asn Gly Ile Met Ala Ser
195 200 205Gln Glu Gln Gly Tyr Lys Asn Ser Leu Asn Asp Phe Phe Thr Trp Val
210 215 220Ala Glu Asn Asn Asp Thr Tyr Gly Leu Gly Thr Thr Pro Leu Ala Val225 230 235 240Tyr Gln Asn Trp Ser Asn Lys Ile Val Ala Glu Thr Gly Thr Asn Pro
245 250 255Thr Val Ala Pro Pro Pro Ala Gln Val Gly Asp Val Pro Pro Ala Phe
260 265 270Ala Trp Ala Gly Gln Ala Gly Ala Asp Met Met Thr Ala Thr Asn Pro
275 280 285Thr Ser Val Gln Leu Gln Gly Leu Ala Thr Gln Leu Leu Asn Pro Gly
290 295 300Ser Asn Gln Ser Leu Ser Pro Leu Leu Asn Val Thr Arg Ala Tyr Ile305 310 315 320Pro Gln Pro Ser Thr Trp Pro Met Leu Ala Gly Ala Ile Ser Gly Gln
325 330 335Thr Pro Ile Pro Asp Val Thr Asp Thr Gly Asp Asp Pro Tyr Val Ile
340 345 350Glu Ser Ile Asn Ala Ser Val Asn Met Gln Arg Met Val Met Cys Asn
355 360 365Glu Asn Thr Val Ala Pro Asp Pro Val Ala Met Ala Arg Met Ala Trp
370 375 380Thr Ser Met Val Thr Gly Asp Val Phe Asp Ile Tyr Ser Val Lys Phe385 390 395 400Ser Ser Gly Gln Ala Cys Ser Gly Ile Thr Pro Thr Ser Gly Arg Gln
405 410 415Pro Thr Asp Gly Ser Gln Leu Ala Val Gln Pro Leu Leu Leu Gln Gly
420 425 430Thr Ser Asp Pro Gln Thr Pro Tyr Trp Thr His Asn Glu Leu Ala Asp
435 440 445Ala Met Asn Ala His Val Val Thr Val Asn Gly Pro Gly His Gly Gln
450 455 460Ser Ile Gly Gly Thr Asn Gln Ala Ile Asn Asp Ile Val Val Asp Tyr465 470 475 480Leu Arg Thr Gly His Thr Asp Ala Thr Trp Val Glu Gly Asn Thr Pro
485 490 495Thr Pro Ile Thr Ala Gly
500<210>135<211>1114<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1114)<223>FRXA00977<400>135gaaaacaaac gtccttgaag ccgtaatgcc ccgttcgaca ataaaaaggg tagtagcagt 60tcttgccgcc tcgactgcgc ttagcccctt tttggtatca atg ccc act gca gca 115
Met Pro Thr Ala Ala
1 5gcg caa gaa aac atc cgc tgg gaa gaa tgc cca cct cag gta gat att 163Ala Gln Glu Asn Ile Arg Trp Glu Glu Cys Pro Pro Gln Val Asp Ile
10 15 20gcc tcc gct caa tgt ggc agc atc gac gtg ccc atg cac tat tct gat 211Ala Ser Ala Gln Cys Gly Ser Ile Asp Val Pro Met His Tyr Ser Asp
25 30 35ccc tca ctt ggc gat atc agc gtg ggc ttt gtc aag gtc cct gcc caa 259Pro Ser Leu Gly Asp Ile Ser Val Gly Phe Val Lys Val Pro Ala Gln
40 45 50ggc gaa aag cac ggc acc atc ttc ggt aac tcc ggt ggc cct ggt ggc 307Gly Glu Lys His Gly Thr Ile Phe Gly Asn Ser Gly Gly Pro Gly Gly
55 60 65gat gcc tat agc ttc ttc ggc agc caa tcc atg aac tgg cca gaa gcc 355Asp Ala Tyr Ser Phe Phe Gly Ser Gln Ser Met Asn Trp Pro Glu Ala70 75 80 85atg tac caa aac tac gac ctc gtt gca gtg cag cct cgc gga atg gtc 403Met Tyr Gln Asn Tyr Asp Leu Val Ala Val Gln Pro Arg Gly Met Val
90 95 100ggc tcc aca ccg gtt aac tgc gac aac atc gca cca gga tac gat ttc 451Gly Ser Thr Pro Val Asn Cys Asp Asn Ile Ala Pro Gly Tyr Asp Phe
105 110 115ctc tcg ctg ctc acc cgc gaa ggc gct ttc gtt aaa gaa tcc tgc gag 499Leu Ser Leu Leu Thr Arg Glu Gly Ala Phe Val Lys Glu Ser Cys Glu
120 125 130atc ggc acc ccc ggc tac acc tcc agc ctg acc acc gac aac acc gcc 547Ile Gly Thr Pro Gly Tyr Thr Ser Ser Leu Thr Thr Asp Asn Thr Ala
135 140 145aac gac tgg gag cgc gtc cgc caa gca ctt ggc gat gac aag atc tcc 595Asn Asp Trp Glu Arg Val Arg Gln Ala Leu Gly Asp Asp Lys Ile Ser150 155 160 165atc ttc gga ctg tcc tac gga acc tac ctc gga tcg gtc tac gcc acc 643Ile Phe Gly Leu Ser Tyr Gly Thr Tyr Leu Gly Ser Val Tyr Ala Thr
170 175 180cgc tac cca cag cac acc gac aag gtt gtc ctc gat tcc gca atg gcg 691Arg Tyr Pro Gln His Thr Asp Lys Val Val Leu Asp Ser Ala Met Ala
185 190 195ccc agc ctg gca tgg aac ggc atc atg gcc tcc caa gaa cag ggc tac 739Pro Ser Leu Ala Trp Asn Gly Ile Met Ala Ser Gln Glu Gln Gly Tyr
200 205 210aaa aac tcc ctc aac gac ttc ttc acc tgg gtt gca gaa aac aac gac 787Lys Asn Ser Leu Asn Asp Phe Phe Thr Trp Val Ala Glu Asn Asn Asp
215 220 225acg tat ggc ctc ggc act acc cca cta gcc gtg tac caa aac tgg tca 835Thr Tyr Gly Leu Gly Thr Thr Pro Leu Ala Val Tyr Gln Asn Trp Ser230 235 240 245aac aag atc gtc gcc gaa acc gga acc aac cca acc gtt gct cca cca 883Asn Lys Ile Val Ala Glu Thr Gly Thr Asn Pro Thr Val Ala Pro Pro
250 255 260cca gca caa gtt ggc gat gtc cca cca gca ttc gca tgg gcc ggc caa 931Pro Ala Gln Val Gly Asp Val Pro Pro Ala Phe Ala Trp Ala Gly Gln
265 270 275gca ggc gca gac atg atg acc gcc acc aac cca acc tcc gtg caa ctc 979Ala Gly Ala Asp Met Met Thr Ala Thr Asn Pro Thr Ser Val Gln Leu
280 285 290cag ggc ctt gcc acc cag ctc cta aac cct gga tcc aac cag tca ctg 1027Gln Gly Leu Ala Thr Gln Leu Leu Asn Pro Gly Ser Asn Gln Ser Leu
295 300 305agc cct ctg ctc aac gtc acc cgc gcc tac att cca cag cca tca acc 1075Ser Pro Leu Leu Asn Val Thr Arg Ala Tyr Ile Pro Gln Pro Ser Thr310 315 320 325tgg ccc atg ctc gca ggc gcc atc tca ggg caa aca ccc 1114Trp Pro Met Leu Ala Gly Ala Ile Ser Gly Gln Thr Pro
330 335<210>136<211>338<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>136Met Pro Thr Ala Ala Ala Gln Glu Asn Ile Arg Trp Glu Glu Cys Pro1 5 10 15Pro Gln Val Asp Ile Ala Ser Ala Gln Cys Gly Ser Ile Asp Val Pro
20 25 30Met His Tyr Ser Asp Pro Ser Leu Gly Asp Ile Ser Val Gly Phe Val
35 40 45Lys Val Pro Ala Gln Gly Glu Lys His Gly Thr Ile Phe Gly Asn Ser
50 55 60Gly Gly Pro Gly Gly Asp Ala Tyr Ser Phe Phe Gly Ser Gln Ser Met65 70 75 80Asn Trp Pro Glu Ala Met Tyr Gln Asn Tyr Asp Leu Val Ala Val Gln
85 90 95Pro Arg Gly Met Val Gly Ser Thr Pro Val Asn Cys Asp Asn Ile Ala
100 105 110Pro Gly Tyr Asp Phe Leu Ser Leu Leu Thr Arg Glu Gly Ala Phe Val
115 120 125Lys Glu Ser Cys Glu Ile Gly Thr Pro Gly Tyr Thr Ser Ser Leu Thr
130 135 140Thr Asp Asn Thr Ala Asn Asp Trp Glu Arg Val Arg Gln Ala Leu Gly145 150 155 160Asp Asp Lys Ile Ser Ile Phe Gly Leu Ser Tyr Gly Thr Tyr Leu Gly
165 170 175Ser Val Tyr Ala Thr Arg Tyr Pro Gln His Thr Asp Lys Val Val Leu
180 185 190Asp Ser Ala Met Ala Pro Ser Leu Ala Trp Asn Gly Ile Met Ala Ser
195 200 205Gln Glu Gln Gly Tyr Lys Asn Ser Leu Asn Asp Phe Phe Thr Trp Val
210 215 220Ala Glu Asn Asn Asp Thr Tyr Gly Leu Gly Thr Thr Pro Leu Ala Val225 230 235 240Tyr Gln Asn Trp Ser Asn Lys Ile Val Ala Glu Thr Gly Thr Asn Pro
245 250 255Thr Val Ala Pro Pro Pro Ala Gln Val Gly Asp Val Pro Pro Ala Phe
260 265 270Ala Trp Ala Gly Gln Ala Gly Ala Asp Met Met Thr Ala Thr Asn Pro
275 280 285Thr Ser Val Gln Leu Gln Gly Leu Ala Thr Gln Leu Leu Asn Pro Gly
290 295 300Ser Asn Gln Ser Leu Ser Pro Leu Leu Asn Val Thr Arg Ala Tyr Ile305 310 315 320Pro Gln Pro Ser Thr Trp Pro Met Leu Ala Gly Ala Ile Ser Gly Gln
325 330 335Thr Pro<210>137<211>269<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(246)<223>FRXA00982<400>137tct caa cta gca gtc caa cca cta ctc ctc cag gga acc agc gac cca 48Ser Gln Leu Ala Val Gln Pro Leu Leu Leu Gln Gly Thr Ser Asp Pro1 5 10 15caa acc cca tac tgg acc cac aac gag ctt gcc gac gcc atg aac gcc 96Gln Thr Pro Tyr Trp Thr His Asn Glu Leu Ala Asp Ala Met Asn Ala
20 25 30cac gtg gtc acc gtc aac gga cca gga cac ggc caa tcc atc ggc ggc 144His Val Val Thr Val Asn Gly Pro Gly His Gly Gln Ser Ile Gly Gly
35 40 45acc aac caa gca atc aac gac att gtt gtg gac tac ctc cgc acc gga 192Thr Asn Gln Ala Ile Asn Asp Ile Val Val Asp Tyr Leu Arg Thr Gly
50 55 60cac acc gac gcc acc tgg gtc gaa ggc aac aca ccc acc cca att acg 240His Thr Asp Ala Thr Trp Val Glu Gly Asn Thr Pro Thr Pro Ile Thr65 70 75 80gct ggc taattgcttt ccacttagta gat 269Ala Gly<210>138<211>82<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>138Ser Gln Leu Ala Val Gln Pro Leu Leu Leu Gln Gly Thr Ser Asp Pro1 5 10 15Gln Thr Pro Tyr Trp Thr His Asn Glu Leu Ala Asp Ala Met Asn Ala
20 25 30His Val Val Thr Val Asn Gly Pro Gly His Gly Gln Ser Ile Gly Gly
35 40 45Thr Asn Gln Ala Ile Asn Asp Ile Val Val Asp Tyr Leu Arg Thr Gly
50 55 60His Thr Asp Ala Thr Trp Val Glu Gly Asn Thr Pro Thr Pro Ile Thr65 70 75 80Ala Gly<210>139<211>1419<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1396)<223>RXA00152<400>139gtcattgata tccaaggcac gaccgcgatt gtatggaaag aagcctaaat ttttaacaat 60caaatagtac tggccattcc caactaaaac tggagtaacg atg aca gga cta atc 115
Met Thr Gly Leu Ile
1 5ctc gcc ata gtt ttc ctg gtc ttt gtc gcc gtc gtg gtg atc aag tcc 163Leu Ala Ile Val Phe Leu Val Phe Val Ala Val Val Val Ile Lys Ser
10 15 20ata gcc ctg att ccc cag ggt gaa gcc gcc gtc att gaa cgc ctt ggt 211Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val Ile Glu Arg Leu Gly
25 30 35agc tac acc cgc acc gtt tca ggt ggc ctg acc ctg ctg gtt cca ttc 259Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr Leu Leu Val Pro Phe
40 45 50gtg gac cga gta cgc gca agg atc gac acc cgt gag cgc gtg gtc tca 307Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg Glu Arg Val Val Ser
55 60 65ttc cca ccg cag gct gtt att acc caa gac aac ctg acc gtg gcc atc 355Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn Leu Thr Val Ala Ile70 75 80 85gat atc gtg gtg acc ttc caa atc aac gaa cca gag cgc gcc atc tac 403Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro Glu Arg Ala Ile Tyr
90 95 100ggc gtg gac aac tac atc gtc ggt gtg gag cag att tct gta gca aca 451Gly Val Asp Ash Tyr Ile Val Gly Val Glu Gln Ile Ser Val Ala Thr
105 110 115ctt cga gac gtt gtc ggt ggc atg acc ctg gaa gaa acc ctc act tca 499Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu Glu Thr Leu Thr Ser
120 125 130cgt gac gtg atc aac cgc cgc ctc cgt ggc gag ctc gat gca gca acc 547Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu Leu Asp Ala Ala Thr
135 140 145acc aaa tgg ggc ctg cgc atc agc cgt gtg gaa cta aag gca att gat 595Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu Leu Lys Ala Ile Asp150 155 160 165ccg cca cca tcc atc cag caa tcg atg gaa aag cag atg aag gca gac 643Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys Gln Met Lys Ala Asp
170 175 180cgt gaa aag cgc gcc acc att ttg acc gca gaa ggt cag cgc gaa gcc 691Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu Gly Gln Arg Glu Ala
185 190 195gac atc aaa act gcc gaa ggt gaa aag caa gcc aag atc ctc caa gct 739Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala Lys Ile Leu Gln Ala
200 205 210gag ggt gaa aag cac gcatcc atc ctg aac gca gaa gca gaa cgc caa 787Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala Glu Ala Glu Arg Gln
215 220 225gcg atg atc ctg cgc gcc gaa ggt gaa cgc gca gca cgc tac ctc cag 835Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala Ala Arg Tyr Leu Gln230 235 240 245gcg cag ggt gaa gcc cga gca atc caa aag gtc aac gca gca atc aag 883Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val Asn Ala Ala Ile Lys
250 255 260tct gcc aag ttg acc cca gag gtt ctt gct tat caa tac ctc gaa aag 931Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr Gln Tyr Leu Glu Lys
265 270 275ctt cct aag atc gca gag ggc aac gcc tcc aag atg tgg gtc atc cca 979Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys Met Trp Val Ile Pro
280 285 290agc cag ttc tcc gat tct ctg gaa ggt ttt gcg aag cag ttc ggc gca 1027Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala Lys Gln Phe Gly Ala
295 300 305aag gat gca gaa ggt gtc ttc cgc tac gaa cca aac acc gtg gat gaa 1075Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro Asn Thr Val Asp Glu310 315 320 325gaa acc cgc gac atc gca aac gcc gac aac gtg gaa gac tgg ttc tcc 1123Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val Glu Asp Trp Phe Ser
330 335 340acc gaa tca gac cct gaa atc gca gca gca gtc gcc gca gca aac gcc 1171Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val Ala Ala Ala Asn Ala
345 350 355gtg gcc aac aag cca gtc gat cca gaa ccc ggt gag atc ctt tcc aag 1219Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly Glu Ile Leu Ser Lys
360 365 370aag acc gca cga cgc gtt gaa cct gaa gca gta ttg gag gct ttg caa 1267Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val Leu Glu Ala Leu Gln
375 380 385aac gga acc act aca caa cct gag gtt gag gca gca cct cct acc gca 1315Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala Ala Pro Pro Thr Ala390 395 400 405aac ttc gcc caa gaa ttc cct gca cca cag gca aac cct gaa gat tac 1363Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala Asn Pro Glu Asp Tyr
410 415 420tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn
425 430cgg1419<210> 140<211> 432<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>140Met Thr Gly Leu Ile Leu Ala Ile Val Phe Leu Val Phe Val Ala Val1 5 10 15Val Val Ile Lys Ser Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val
20 25 30Ile Glu Arg Leu Gly Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr
35 40 45Leu Leu Val Pro Phe Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg
50 55 60Glu Arg Val Val Ser Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn65 70 75 80Leu Thr Val Ala Ile Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro
85 90 95Glu Arg Ala Ile Tyr Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln
100 105 110Ile Ser Val Ala Thr Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu
115 120 125Glu Thr Leu Thr Ser Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu
130 135 140Leu Asp Ala Ala Thr Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu145 150 155 160Leu Lys Ala Ile Asp Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys
165 170 175Gln Met Lys Ala Asp Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu
180 185 190Gly Gln Arg Glu Ala Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala
195 200 205Lys Ile Leu Gln Ala Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala
210 215 220Glu Ala Glu Arg Gln Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala225 230 235 240Ala Arg Tyr Leu Gln Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val
245 250 255Asn Ala Ala Ile Lys Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr
260 265 270Gln Tyr Leu Glu Lys Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys
275 280 285Met Trp Val Ile Pro Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala
290 295 300Lys Gln Phe Gly Ala Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro305 310 315 320Asn Thr Val Asp Glu Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val
325 330 335Glu Asp Trp Phe Ser Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val
340 345 350Ala Ala Ala Asn Ala Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly
355 360 365Glu Ile Leu Ser Lys Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val
370 375 380Leu Glu Ala Leu Gln Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala385 390 395 400Ala Pro Pro Thr Ala Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala
405 410 415Asn Pro Glu Asp Tyr Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn
420 425 430<210>141<211>1098<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1075)<223>RXA02558<400>141ccctcccgaa attagaaatc cccttttaat gtgatatcac tttctgttaa actgatatca 60cattcttttt cagcacccca gacttaaaag gagcaccacc atg agc acc ata gaa 115
Met Ser Thr Ile Glu
1 5gag cgc act cct gga gct gtc gcc aca gaa cca gtg gga cac gaa ggc 163Glu Arg Thr Pro Gly Ala Val Ala Thr Glu Pro Val Gly His Glu Gly
10 15 20gca cgc gtc agc att aat gag aag aac gtg tgg tct ttg ggc gca ggt 211Ala Arg Val Ser Ile Asn Glu Lys Asn Val Trp Ser Leu Gly Ala Gly
25 30 35cca gca gct ttc gca ctg ctc gca atg att gtg ctc atg att gcc agt 259Pro Ala Ala Phe Ala Leu Leu Ala Met Ile Val Leu Met Ile Ala Ser
40 45 50gga gtt ttc ttc gct caa tcc atc aac act tta gaa aac gat ggc ggt 307Gly Val Phe Phe Ala Gln Ser Ile Asn Thr Leu Glu Asn Asp Gly Gly
55 60 65gga aca ctt gcg gtt acg gga ctg att gcc agc atc gtc gtt ttc act 355Gly Thr Leu Ala Val Thr Gly Leu Ile Ala Ser Ile Val Val Phe Thr70 75 80 85gtt gca ttg gtg gtc acc ata act tcg gtg aag gtg gtc agc cct gga 403Val Ala Leu Val Val Thr Ile Thr Ser Val Lys Val Val Ser Pro Gly
90 95 100cat act ctg act gtg cag ttc ttt gga cga tac atc gga acc ctg cgt 451His Thr Leu Thr Val Gln Phe Phe Gly Arg Tyr Ile Gly Thr Leu Arg
105 110 115cga act ggg ttg tct ttc gtt ccc cca ctg tct gtg acg aag aaa gtg 499Arg Thr Gly Leu Ser Phe Val Pro Pro Leu Ser Val Thr Lys Lys Val
120 125 130tcc gtg agg gtc cga aac ttt gaa acc aac gaa gcc aaa gtt aat gac 547Ser Val Arg Val Arg Asn Phe Glu Thr Asn Glu Ala Lys Val Asn Asp
135 140 145tac aac ggc aac ccc atc aac att gca gcg atc atc gtg tgg cag gta 595Tyr Asn Gly Asn Pro Ile Asn Ile Ala Ala Ile Ile Val Trp Gln Val150 155 160 165gcc gat act gca cag gct agc ttc tct gtg gag gat ttc gaa gag ttc 643Ala Asp Thr Ala Gln Ala Ser Phe Ser Val Glu Asp Phe Glu Glu Phe
170 175 180ctg cac cag cag gcc gag tcc gca ctg cgt cac gtg gca acc cag cac 691Leu His Gln Gln Ala Glu Ser Ala Leu Arg His Val Ala Thr Gln His
185 190 195ccc tat gat tcc cca gtt gat ggt cgt gtt tcc ttg cgt ggc gct acc 739Pro Tyr Asp Ser Pro Val Asp Gly Arg Val Ser Leu Arg Gly Ala Thr
200 205 210gat gag gtc agt gaa gaa ctc gca gat gag gtg gca caa cga gca gct 787Asp Glu Val Ser Glu Glu Leu Ala Asp Glu Val Ala Gln Arg Ala Ala
215 220 225gtt gca ggt ctt gaa atc gtc gaa gcc cgc atc tct tcc ttg agc tac 835Val Ala Gly Leu Glu Ile Val Glu Ala Arg Ile Ser Ser Leu Ser Tyr230 235 240 245gca ccg gaa att gcc cag gcg atg ctg cag cgc cag cag gct tcc gcg 883Ala Pro Glu Ile Ala Gln Ala Met Leu Gln Arg Gln Gln Ala Ser Ala
250 255 260att gtt gat gcc cgc gaa aag atc gtc gag ggc gct gtc acc atg gtg 931Ile Val Asp Ala Arg Glu Lys Ile Val Glu Gly Ala Val Thr Met Val
265 270 275gaa acc gca ctt gac cag ctt gag caa cgt gaa att gtg gat ttg gat 979Glu Thr Ala Leu Asp Gln Leu Glu Gln Arg Glu Ile Val Asp Leu Asp
280 285 290cca gag cga cgc gcc gcg atg gtt tcc aac ctg ttg gtt gtg ttg tgt 1027Pro Glu Arg Arg Ala Ala Met Val Ser Asn Leu Leu Val Val Leu Cys
295 300 305tcc gac acc aat gct cag cca atc gtc aac gcc ggt agc ctc tac caa 1075Ser Asp Thr Asn Ala Gln Pro Ile Val Asn Ala Gly Ser Leu Tyr Gln310 315 320 325taagacaatg gcccgcaaac agg 1098<210>142<211>325<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>142Met Ser Thr Ile Glu Glu Arg Thr Pro Gly Ala Val Ala Thr Glu Pro1 5 10 15Val Gly His Glu Gly Ala Arg Val Ser Ile Asn Glu Lys Asn Val Trp
20 25 30Ser Leu Gly Ala Gly Pro Ala Ala Phe Ala Leu Leu Ala Met Ile Val
35 40 45Leu Met Ile Ala Ser Gly Val Phe Phe Ala Gln Ser Ile Asn Thr Leu
50 55 60Glu Asn Asp Gly Gly Gly Thr Leu Ala Val Thr Gly Leu Ile Ala Ser65 70 75 80Ile Val Val Phe Thr Val Ala Leu Val Val Thr Ile Thr Ser Val Lys
85 90 95Val Val Ser Pro Gly His Thr Leu Thr Val Gln Phe Phe Gly Arg Tyr
100 105 110Ile Gly Thr Leu Arg Arg Thr Gly Leu Ser Phe Val Pro Pro Leu Ser
115 120 125Val Thr Lys Lys Val Ser Val Arg Val Arg Asn Phe Glu Thr Asn Glu
130 135 140Ala Lys Val Asn Asp Tyr Asn Gly Asn Pro Ile Asn Ile Ala Ala Ile145 150 155 160Ile Val Trp Gln Val Ala Asp Thr Ala Gln Ala Ser Phe Ser Val Glu
165 170 175Asp Phe Glu Glu Phe Leu His Gln Gln Ala Glu Ser Ala Leu Arg His
180 185 190Val Ala Thr Gln His Pro Tyr Asp Ser Pro Val Asp Gly Arg Val Ser
195 200 205Leu Arg Gly Ala Thr Asp Glu Val Ser Glu Glu Leu Ala Asp Glu Val
210 215 220Ala Gln Arg Ala Ala Val Ala Gly Leu Glu Ile Val Glu Ala Arg Ile225 230 235 240Ser Ser Leu Ser Tyr Ala Pro Glu Ile Ala Gln Ala Met Leu Gln Arg
245 250 255Gln Gln Ala Ser Ala Ile Val Asp Ala Arg Glu Lys Ile Val Glu Gly
260 265 270Ala Val Thr Met Val Glu Thr Ala Leu Asp Gln Leu Glu Gln Arg Glu
275 280 285Ile Val Asp Leu Asp Pro Glu Arg Arg Ala Ala Met Val Ser Asn Leu
290 295 300Leu Val Val Leu Cys Ser Asp Thr Asn Ala Gln Pro Ile Val Asn Ala305 310 315 320Gly Ser Leu Tyr Gln
325<210>143<211>798<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(775)<223>RXA00500<400>143caccagccag catgaacaca atggacttcg tgccagctca ggcgcagtac tgaagcacct 60tttcgatctg gcccacggcc gagaggtacg ctgattcctc gtg tta gta cta gcc 115
Val Leu Val Leu Ala
1 5cta gac acc tca acc cct gac ctg atc gtc ggc gtc gtc gac tcc gac 163Leu Asp Thr Ser Thr Pro Asp Leu Ile Val Gly Val Val Asp Ser Asp
10 15 20acc gga aac acc cgc gcc gaa acc atc atc gag gac acc cgc gca cac 211Thr Gly Asn Thr Arg Ala Glu Thr Ile Ile Glu Asp Thr Arg Ala His
25 30 35aac gag cag ctc acg ccc acc gtc cag aag acg ctt ctc gac gcc aac 259Asn Glu Gln Leu Thr Pro Thr Val Gln Lys Thr Leu Leu Asp Ala Asn
40 45 50ttg agc ttt tca gat atc gac gcg atc gtc gtg ggt tgc ggc ccg gga 307Leu Ser Phe Ser Asp Ile Asp Ala Ile Val Val Gly Cys Gly Pro Gly
55 60 65ccg ttc act gga ctt cga gta ggc atg gtg tcc ggc gca gcg ttc ggt 355Pro Phe Thr Gly Leu Arg Val Gly Met Val Ser Gly Ala Ala Phe Gly70 75 80 85gat gcc ctg gga atc cct gtc tat gga gtc tgc tca ctc gac gcg atc 403Asp Ala Leu Gly Ile Pro Val Tyr Gly Val Cys Ser Leu Asp Ala Ile
90 95 100gct cac aat att ggt gca cgc aac atc ccg cac gca tta gtt gcc act 451Ala His Asn Ile Gly Ala Arg Asn Ile Pro His Ala Leu Val Ala Thr
105 110 115gat gcg cgc cgc cgt gaa atc tac tgg gca acc tac cgc tcc ggc gaa 499Asp Ala Arg Arg Arg Glu Ile Tyr Trp Ala Thr Tyr Arg Ser Gly Glu
120 125 130cgt gat cag gga cca gat gtc atc gca cca gca aac atc cag atc agc 547Arg Asp Gln Gly Pro Asp Val Ile Ala Pro Ala Asn Ile Gln Ile Ser
135 140 145ggc gct gta gac acc att tcg att cct gag cac ctg gtg gaa aaa ctc 595Gly Ala Val Asp Thr Ile Ser Ile Pro Glu His Leu Val Glu Lys Leu150 155 160 165cca gaa gaa ctc cag aat gtc acc atg cat agc ggc aaa cct gcc ccc 643Pro Glu Glu Leu Gln Asn Val Thr Met His Ser Gly Lys Pro Ala Pro
170 175 180gca agc ttg gtg gca gtg gct gat ttc agt gtg gaa cca caa cca ttg 691Ala Ser Leu Val Ala Val Ala Asp Phe Ser Val Glu Pro Gln Pro Leu
185 190 195gtt cct ctt tac ctg cgc cgc cca gat gcc aaa gaa cca aaa cca aaa 739Val Pro Leu Tyr Leu Arg Arg Pro Asp Ala Lys Glu Pro Lys Pro Lys
200 205 210cct aaa tct gca gcc atc ccc gag gtg gat ctt tca tgagtgaaca 785Pro Lys Ser Ala Ala Ile Pro Glu Val Asp Leu Ser
215 220 225attcgagcta cgg 798<210>144<211>225<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>144Val Leu Val Leu Ala Leu Asp Thr Ser Thr Pro Asp Leu Ile Val Gly1 5 10 15Val Val Asp Ser Asp Thr Gly Asn Thr Arg Ala Glu Thr Ile Ile Glu
20 25 30Asp Thr Arg Ala His Asn Glu Gln Leu Thr Pro Thr Val Gln Lys Thr
35 40 45Leu Leu Asp Ala Asn Leu Ser Phe Ser Asp Ile Asp Ala Ile Val Val
50 55 60Gly Cys Gly Pro Gly Pro Phe Thr Gly Leu Arg Val Gly Met Val Ser65 70 75 80Gly Ala Ala Phe Gly Asp Ala Leu Gly Ile Pro Val Tyr Gly Val Cys
85 90 95Ser Leu Asp Ala Ile Ala His Asn Ile Gly Ala Arg Asn Ile Pro His
100 105 110Ala Leu Val Ala Thr Asp Ala Arg Arg Arg Glu Ile Tyr Trp Ala Thr
115 120 125Tyr Arg Ser Gly Glu Arg Asp Gln Gly Pro Asp Val Ile Ala Pro Ala
130 135 140Asn Ile Gln Ile Ser Gly Ala Val Asp Thr Ile Ser Ile Pro Glu His145 150 155 160Leu Val Glu Lys Leu Pro Glu Glu Leu Gln Asn Val Thr Met His Ser
165 170 175Gly Lys Pro Ala Pro Ala Ser Leu Val Ala Val Ala Asp Phe Ser Val
180 185 190Glu Pro Gln Pro Leu Val Pro Leu Tyr Leu Arg Arg Pro Asp Ala Lys
195 200 205Glu Pro Lys Pro Lys Pro Lys Ser Ala Ala Ile Pro Glu Val Asp Leu
210 215 220Ser225<210>145<211>630<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(607)<223>RXA00501<400>145tggaaccaca accattggtt cctctttacc tgcgccgccc agatgccaaa gaaccaaaac 60caaaacctaa atctgcagcc atccccgagg tggatctttc atg agt gaa caa ttc 115
Met Ser Glu Gln Phe
1 5gag cta cgg gaa ctc cgc agg gaa gac gcg ggg cgc tgc gcc gac ctg 163Glu Leu Arg Glu Leu Arg Arg Glu Asp Ala Gly Arg Cys Ala Asp Leu
10 15 20gag caa atc ctg ttc cca ggt gat aac ccc tgg cca cgt gat gtc ttt 211Glu Gln Ile Leu Phe Pro Gly Asp Asn Pro Trp Pro Arg Asp Val Phe
25 30 35gcc gtg gag ttt tcc cac ccc acc aat ttc tac atc ggc gct ttc gac 259Ala Val Glu Phe Ser His Pro Thr Asn Phe Tyr Ile Gly Ala Phe Asp
40 45 50gaa gga tac ttg gtg gcg tac gca ggt ctt gcc atg atg gga cct gcg 307Glu Gly Tyr Leu Val Ala Tyr Ala Gly Leu Ala Met Met Gly Pro Ala
55 60 65gat gat cca gag ttt gaa atc cac acc att ggt gtc gat ccg gaa ttc 355Asp Asp Pro Glu Phe Glu Ile His Thr Ile Gly Val Asp Pro Glu Phe70 75 80 85caa aga aaa ggc ttg gga cgc gta ctc atg gat caa atg atg cat gca 403Gln Arg Lys Gly Leu Gly Arg Val Leu Met Asp Gln Met Met His Ala
90 95 100gcg gac agc cac gac ggt cca gtt ttc ttg gaa gtc cgc acc gac aac 451Ala Asp Ser His Asp Gly Pro Val Phe Leu Glu Val Arg Thr Asp Asn
105 110 115gta ccc gcg att tcc atg tac gag gct ttc ggc ttt aaa acc ttg gcc 499Val Pro Ala Ile Ser Met Tyr Glu Ala Phe Gly Phe Lys Thr Leu Ala
120 125 130gtg cgc aaa aac tac tac cgg cca tcc gga gct gac gcc tac acc atg 547Val Arg Lys Asn Tyr Tyr Arg Pro Ser Gly Ala Asp Ala Tyr Thr Met
135 140 145caa cgc cca cgc ttg agc gat cgc aaa gat caa cag aca gac aca gag 595Gln Arg Pro Arg Leu Ser Asp Arg Lys Asp Gln Gln Thr Asp Thr Glu150 155 160 165ggg aca ccc agc taaaccatga tcgttttggg aat 630Gly Thr Pro Ser<210>146<211>169<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>146Met Ser Glu Gln Phe Glu Leu Arg Glu Leu Arg Arg Glu Asp Ala Gly1 5 10 15Arg Cys Ala Asp Leu Glu Gln Ile Leu Phe Pro Gly Asp Asn Pro Trp
20 25 30Pro Arg Asp Val Phe Ala Val Glu Phe Ser His Pro Thr Asn Phe Tyr
35 40 45Ile Gly Ala Phe Asp Glu Gly Tyr Leu Val Ala Tyr Ala Gly Leu Ala
50 55 60Met Met Gly Pro Ala Asp Asp Pro Glu Phe Glu Ile His Thr Ile Gly65 70 75 80Val Asp Pro Glu Phe Gln Arg Lys Gly Leu Gly Arg Val Leu Met Asp
85 90 95Gln Met Met His Ala Ala Asp Ser His Asp Gly Pro Val Phe Leu Glu
100 105 110Val Arg Thr Asp Asn Val Pro Ala Ile Ser Met Tyr Glu Ala Phe Gly
115 120 125Phe Lys Thr Leu Ala Val Arg Lys Asn Tyr Tyr Arg Pro Ser Gly Ala
130 135 140Asp Ala Tyr Thr Met Gln Arg Pro Arg Leu Ser Asp Arg Lys Asp Gln145 150 155 160Gln Thr Asp Thr Glu Gly Thr Pro Ser
165<210>147<211>1155<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1132)<223>RXA00502<400>147ctaccggcca tccggagctg acgcctacac catgcaacgc ccacgcttga gcgatcgcaa 60agatcaacag acagacacag aggggacacc cagctaaacc atg atc gtt ttg gga 115
Met Ile Val Leu Gly
1 5att gaa agc tcc tgc gat gaa aca ggc gta ggc gta gtc aaa ctt gac 163Ile Glu Ser Ser Cys Asp Glu Thr Gly Val Gly Val Val Lys Leu Asp
10 15 20ggc gaa gga aac cta gag atc ctc gcc gac tca gtg gcc tcc tcc atg 211Gly Glu Gly Asn Leu Glu Ile Leu Ala Asp Ser Val Ala Ser Ser Met
25 30 35caa gaa cat gcc cgc ttt ggt ggc gtc gtg cca gaa atc gcc tcc cgg 259Gln Glu His Ala Arg Phe Gly Gly Val Val Pro Glu Ile Ala Ser Arg
40 45 50gcg cac ctg gaa tct atg gtc ccc gtg atg cgt gaa gcg ttg agg cag 307Ala His Leu Glu Ser Met Val Pro Val Met Arg Glu Ala Leu Arg Gln
55 60 65gcg ggc gtc gac agg cca gat gct gtg gct gca acc gtg ggc cct ggt 355Ala Gly Val Asp Arg Pro Asp Ala Val Ala Ala Thr Val Gly Pro Gly70 75 80 85ttg gcg ggc gcg ctg ctc gtt gga gcc agc gct gcg aag gcg tat gcc 403Leu Ala Gly Ala Leu Leu Val Gly Ala Ser Ala Ala Lys Ala Tyr Ala
90 95 100gct gcg tgg gga gtt ccg ttt tac gcg gtc aac cac ctg ggc gga cac 451Ala Ala Trp Gly Val Pro Phe Tyr Ala Val Asn His Leu Gly Gly His
105 110 115gtc gcc gtg gcc aat ctg gaa ggt gaa act ctt cca cac gcg gtg gct 499Val Ala Val Ala Asn Leu Glu Gly Glu Thr Leu Pro His Ala Val Ala
120 125 130ttg ctg gtt tcc ggc gga cac act caa ttg ttg gaa gtc gac gcg gtg 547Leu Leu Val Ser Gly Gly His Thr Gln Leu Leu Glu Val Asp Ala Val
135 140 145gga tta ccc atg aag gaa ttg gga tcc acc ctc gac gat gcc gct ggc 595Gly Leu Pro Met Lys Glu Leu Gly Ser Thr Leu Asp Asp Ala Ala Gly150 155 160 165gaa gcc tat gac aaa gtc tca agg ctg ttg gga ttg ggc tac cca ggc 643Glu Ala Tyr Asp Lys Val Ser Arg Leu Leu Gly Leu Gly Tyr Pro Gly
170 175 180ggc ccc atc att gat aaa ttg gcg cgc cgg ggt aat cca gag gcc att 691Gly Pro Ile Ile Asp Lys Leu Ala Arg Arg Gly Asn Pro Glu Ala Ile
185 190 195gct ttc ccc cgc gga ttg atg aaa aag tcg gat tct cgg cat gat ttc 739Ala Phe Pro Arg Gly Leu Met Lys Lys Ser Asp Ser Arg His Asp Phe
200 205 210agc ttt tcc ggt ttg aaa acc tcc gtt gcc cgc tac gtg gaa gct gcg 787Ser Phe Ser Gly Leu Lys Thr Ser Val Ala Arg Tyr Val Glu Ala Ala
215 220 225gaa aga aac ggt gaa gtt att tcc gtg gag gac gtc tgc gca tca ttc 835Glu Arg Asn Gly Glu Val Ile Ser Val Glu Asp Val Cys Ala Ser Phe230 235 240 245caa gaa gcg gtg tgt gat gtg ttg acg ttt aag gcc gtg cgt gcg tgc 883Gln Glu Ala Val Cys Asp Val Leu Thr Phe Lys Ala Val Arg Ala Cys
250 255 260cgc gat gtc ggt gcg aag gtg ctg ctg ttg ggt gga gga gtg gct gcc 931Arg Asp Val Gly Ala Lys Val Leu Leu Leu Gly Gly Gly Val Ala Ala
265 270 275aac tct cgt ctg cgg gag ctt gct caa gaa cgt tgc gat aaa gcc gac 979Asn Ser Arg Leu Arg Glu Leu Ala Gln Glu Arg Cys Asp Lys Ala Asp
280 285 290atc gaa ctc cgg gtt cct cgt ttc aat ttg tgc acc gat aat ggt gtc 1027Ile Glu Leu Arg Val Pro Arg Phe Asn Leu Cys Thr Asp Asn Gly Val
295 300 305atg att gca gcg ttg gcg gct caa aga atc cac gaa ggt gcc caa gaa 1075Met Ile Ala Ala Leu Ala Ala Gln Arg Ile His Glu Gly Ala Gln Glu310 315 320 325tca cca att tcg gtc gga act gat cct tct ttg tcc gtt gag acc cca 1123Ser Pro Ile Ser Val Gly Thr Asp Pro Ser Leu Ser Val Glu Thr Pro
330 335 340cag gtg ttt taaacattta gtattagttc cat 1155Gln Val Phe<210>148<211>344<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>148Met Ile Val Leu Gly Ile Glu Ser Ser Cys Asp Glu Thr Gly Val Gly1 5 10 15Val Val Lys Leu Asp Gly Glu Gly Asn Leu Glu Ile Leu Ala Asp Ser
20 25 30Val Ala Ser Ser Met Gln Glu His Ala Arg Phe Gly Gly Val Val Pro
35 40 45Glu Ile Ala Ser Arg Ala His Leu Glu Ser Met Val Pro Val Met Arg
50 55 60Glu Ala Leu Arg Gln Ala Gly Val Asp Arg Pro Asp Ala Val Ala Ala65 70 75 80Thr Val Gly Pro Gly Leu Ala Gly Ala Leu Leu Val Gly Ala Ser Ala
85 90 95Ala Lys Ala Tyr Ala Ala Ala Trp Gly Val Pro Phe Tyr Ala Val Asn
100 105 110His Leu Gly Gly His Val Ala Val Ala Asn Leu Glu Gly Glu Thr Leu
115 120 125Pro His Ala Val Ala Leu Leu Val Ser Gly Gly His Thr Gln Leu Leu
130 135 140Glu Val Asp Ala Val Gly Leu Pro Met Lys Glu Leu Gly Ser Thr Leu145 150 155 160Asp Asp Ala Ala Gly Glu Ala Tyr Asp Lys Val Ser Arg Leu Leu Gly
165 170 175Leu Gly Tyr Pro Gly Gly Pro Ile Ile Asp Lys Leu Ala Arg Arg Gly
180 185 190Asn Pro Glu Ala Ile Ala Phe Pro Arg Gly Leu Met Lys Lys Ser Asp
195 200 205Ser Arg His Asp Phe Ser Phe Ser Gly Leu Lys Thr Ser Val Ala Arg
210 215 220Tyr Val Glu Ala Ala Glu Arg Asn Gly Glu Val Ile Ser Val Glu Asp225 230 235 240Val Cys Ala Ser Phe Gln Glu Ala Val Cys Asp Val Leu Thr Phe Lys
245 250 255Ala Val Arg Ala Cys Arg Asp Val Gly Ala Lys Val Leu Leu Leu Gly
260 265 270Gly Gly Val Ala Ala Asn Ser Arg Leu Arg Glu Leu Ala Gln Glu Arg
275 280 285Cys Asp Lys Ala Asp Ile Glu Leu Arg Val Pro Arg Phe Asn Leu Cys
290 295 300Thr Asp Asn Gly Val Met Ile Ala Ala Leu Ala Ala Gln Arg Ile His305 310 315 320Glu Gly Ala Gln Glu Ser Pro Ile Ser Val Gly Thr Asp Pro Ser Leu
325 330 335Ser Val Glu Thr Pro Gln Val Phe
340<210>149<211>888<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(865)<223>RXN02589<400>149gcctaaattg cagcgagagg tctaaaaggt agtgctctag ggattcatcc aaactcacga 60atattgaagt tttaaagttg aacaggaaaa ataacaaata atg tct att tct gat 115
Met Ser Ile Ser Asp
1 5aat tcc cgc gat caa tta gga gaa ctg cca gct ggt cgg cct ctc caa 163Asn Ser Arg Asp Gln Leu Gly Glu Leu Pro Ala Gly Arg Pro Leu Gln
10 15 20tcc gat ttt gat aat gac ctc gac tac cca cgt cta ggc agt gtc acg 211Ser Asp Phe Asp Asn Asp Leu Asp Tyr Pro Arg Leu Gly Ser Val Thr
25 30 35ttt agg cgt ggc acc ctc act gaa aac cag caa acc atg tgg gat gaa 259Phe Arg Arg Gly Thr Leu Thr Glu Asn Gln Gln Thr Met Trp Asp Glu
40 45 50aag tgg cct gaa ctg ggt cgc gtc ctc gaa gat gag ctg att gat gtt 307Lys Trp Pro Glu Leu Gly Arg Val Leu Glu Asp Glu Leu Ile Asp Val
55 60 65gat gcg tgg ttc ggg cgc gaa ggc gca aaa acc atc gta gag atc ggc 355Asp Ala Trp Phe Gly Arg Glu Gly Ala Lys Thr Ile Val Glu Ile Gly70 75 80 85tct ggc act gga act tcg act gct gcc atg gct cca ctt gag gct gat 403Ser Gly Thr Gly Thr Ser Thr Ala Ala Met Ala Pro Leu Glu Ala Asp
90 95 100acc aac att gtc gcc gtc gaa cta tac aag ccg ggc ttg gcc aag ttg 451Thr Asn Ile Val Ala Val Glu Leu Tyr Lys Pro Gly Leu Ala Lys Leu
105 110 115atg ggc tct gtt gtc cgt gga gag atc gac aac gtg cgc atg gtc cgc 499Met Gly Ser Val Val Arg Gly Glu Ile Asp Asn Val Arg Met Val Arg
120 125 130gga gac ggc atc gag gtg ctc aac cgc atg ttt gcc gat ggg tcc ctg 547Gly Asp Gly Ile Glu Val Leu Asn Arg Met Phe Ala Asp Gly Ser Leu
135 140 145gac ggc atc cgc gta tac ttc ccg gac cct tgg cca aag gcg cgc cac 595Asp Gly Ile Arg Val Tyr Phe Pro Asp Pro Trp Pro Lys Ala Arg His150 155 160 165aac aag cgc cgc atc atc cag tct ggt ccg ctg aac ctg ttt gca aag 643Asn Lys Arg Arg Ile Ile Gln Ser Gly Pro Leu Asn Leu Phe Ala Lys
170 175 180aag ctc aag cca ggt gga gtt ctg cac gtt gct acc gac cac gct gat 691Lys Leu Lys Pro Gly Gly Val Leu His Val Ala Thr Asp His Ala Asp
185 190 195tac gca gag tgg atc aat gag cta gtt gag gtc gaa cca ctg ctt gag 739Tyr Ala Glu Trp Ile Asn Glu Leu Val Glu Val Glu Pro Leu Leu Glu
200 205 210tac aaa ggc tgg cca tgg gag gaa tgc cct cag ctg act gac cgt cag 787Tyr Lys Gly Trp Pro Trp Glu Glu Cys Pro Gln Leu Thr Asp Arg Gln
215 220 225gtc atc acc aag ttt gaa ggc aaa ggc ttg gaa aaa gat cac gtg atc 835Val Ile Thr Lys Phe Glu Gly Lys Gly Leu Glu Lys Asp His Val Ile230 235 240 245aat gag tac ttg tgg cag aag gtg caa aac taatgtctga tgtgcatgag 885Asn Glu Tyr Leu Trp Gln Lys Val Gln Asn
250 255gtc 888<210>150<211>255<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>150Met Ser Ile Ser Asp Asn Ser Arg Asp Gln Leu Gly Glu Leu Pro Ala1 5 10 15Gly Arg Pro Leu Gln Ser Asp Phe Asp Asn Asp Leu Asp Tyr Pro Arg
20 25 30Leu Gly Ser Val Thr Phe Arg Arg Gly Thr Leu Thr Glu Asn Gln Gln
35 40 45Thr Met Trp Asp Glu Lys Trp Pro Glu Leu Gly Arg Val Leu Glu Asp
50 55 60Glu Leu Ile Asp Val Asp Ala Trp Phe Gly Arg Glu Gly Ala Lys Thr65 70 75 80Ile Val Glu Ile Gly Ser Gly Thr Gly Thr Ser Thr Ala Ala Met Ala
85 90 95Pro Leu Glu Ala Asp Thr Asn Ile Val Ala Val Glu Leu Tyr Lys Pro
100 105 110Gly Leu Ala Lys Leu Met Gly Ser Val Val Arg Gly Glu Ile Asp Asn
115 120 125Val Arg Met Val Arg Gly Asp Gly Ile Glu Val Leu Asn Arg Met Phe
130 135 140Ala Asp Gly Ser Leu Asp Gly Ile Arg Val Tyr Phe Pro Asp Pro Trp145 150 155 160Pro Lys Ala Arg His Asn Lys Arg Arg Ile Ile Gln Ser Gly Pro Leu
165 170 175Asn Leu Phe Ala Lys Lys Leu Lys Pro Gly Gly Val Leu His Val Ala
180 185 190Thr Asp His Ala Asp Tyr Ala Glu Trp Ile Asn Glu Leu Val Glu Val
195 200 205Glu Pro Leu Leu Glu Tyr Lys Gly Trp Pro Trp Glu Glu Cys Pro Gln
210 215 220Leu Thr Asp Arg Gln Val Ile Thr Lys Phe Glu Gly Lys Gly Leu Glu225 230 235 240Lys Asp His Val Ile Asn Glu Tyr Leu Trp Gln Lys Val Gln Asn
245 250 255<210>151<211>888<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(865)<223>FRXA02589<400>151gcctaaattg cagcgagagg tctaaaaggt agtgctctag ggattcatcc aaactcacga 60atattgaagt tttaaagttg aacaggaaaa ataacaaata atg tct att tct gat 115
Met Ser Ile Ser Asp
1 5aat tcc cgc gat caa tta gga gaa ctg cca gct ggt cgg cct ctc caa 163Asn Ser Arg Asp Gln Leu Gly Glu Leu Pro Ala Gly Arg Pro Leu Gln
10 15 20tcc gat ttt gat aat gac ctc gac tac cca cgt cta ggc agt gtc acg 211Ser Asp Phe Asp Asn Asp Leu Asp Tyr Pro Arg Leu Gly Ser Val Thr
25 30 35ttt agg cgt ggc acc ctc act gaa aac cag caa acc atg tgg gat gaa 259Phe Arg Arg Gly Thr Leu Thr Glu Asn Gln Gln Thr Met Trp Asp Glu
40 45 50aag tgg cct gaa ctg ggt cgc gtc ctc gaa gat gag ctg att gat gtt 307Lys Trp Pro Glu Leu Gly Arg Val Leu Glu Asp Glu Leu Ile Asp Val
55 60 65gat gcg tgg ttc ggg cgc gaa ggc gca aaa acc atc gta gag atc ggc 355Asp Ala Trp Phe Gly Arg Glu Gly Ala Lys Thr Ile Val Glu Ile Gly70 75 80 85tct ggc act gga act tcg act gct gcc atg gct cca ctt gag gct gat 403Ser Gly Thr Gly Thr Ser Thr Ala Ala Met Ala Pro Leu Glu Ala Asp
90 95 100acc aac att gtc gcc gtc gaa cta tac aag ccg ggc ttg gcc aag ttg 451Thr Asn Ile Val Ala Val Glu Leu Tyr Lys Pro Gly Leu Ala Lys Leu
105 110 115atg ggc tct gtt gtc cgt gga gag atc gac aac gtg cgc atg gtc cgc 499Met Gly Ser Val Val Arg Gly Glu Ile Asp Asn Val Arg Met Val Arg
120 125 130gga gac ggc atc gag gtg ctc aac cgc atg ttt gcc gat ggg tcc ctg 547Gly Asp Gly Ile Glu Val Leu Asn Arg Met Phe Ala Asp Gly Ser Leu
135 140 145gac ggc atc cgc gta tac ttc ccg gac cct tgg cca aag gcg cgc cac 595Asp Gly Ile Arg Val Tyr Phe Pro Asp Pro Trp Pro Lys Ala Arg His150 155 160 165aac aag cgc cgc atc atc cag tct ggt ccg ctg aac ctg ttt gca aag 643Asn Lys Arg Arg Ile Ile Gln Ser Gly Pro Leu Asn Leu Phe Ala Lys
170 175 180aag ctc aag cca ggt gga gtt ctg cac gtt gct acc gac cac gct gat 691Lys Leu Lys Pro Gly Gly Val Leu His Val Ala Thr Asp His Ala Asp
185 190 195tac gca gag tgg atc aat gag cta gtt gag gtc gaa cca ctg ctt gag 739Tyr Ala Glu Trp Ile Asn Glu Leu Val Glu Val Glu Pro Leu Leu Glu
200 205 210tac aaa ggc tgg cca tgg gag gaa tgc cct cag ctg act gac cgt cag 787Tyr Lys Gly Trp Pro Trp Glu Glu Cys Pro Gln Leu Thr Asp Arg Gln
215 220 225gtc atc acc aag ttt gaa ggc aaa ggc ttg gaa aaa gat cac gtg atc 835Val Ile Thr Lys Phe Glu Gly Lys Gly Leu Glu Lys Asp His Val Ile230 235 240 245aat gag tac ttg tgg cag aag gtg caa aac taatgtctga tgtgcatgag 885Asn Glu Tyr Leu Trp Gln Lys Val Gln Asn
250 255gtc 888<210>152<211>255<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>152Met Ser Ile Ser Asp Asn Ser Arg Asp Gln Leu Gly Glu Leu Pro Ala1 5 10 15Gly Arg Pro Leu Gln Ser Asp Phe Asp Asn Asp Leu Asp Tyr Pro Arg
20 25 30Leu Gly Ser Val Thr Phe Arg Arg Gly Thr Leu Thr Glu Asn Gln Gln
35 40 45Thr Met Trp Asp Glu Lys Trp Pro Glu Leu Gly Arg Val Leu Glu Asp
50 55 60Glu Leu Ile Asp Val Asp Ala Trp Phe Gly Arg Glu Gly Ala Lys Thr65 70 75 80Ile Val Glu Ile Gly Ser Gly Thr Gly Thr Ser Thr Ala Ala Met Ala
85 90 95Pro Leu Glu Ala Asp Thr Asn Ile Val Ala Val Glu Leu Tyr Lys Pro
100 105 110Gly Leu Ala Lys Leu Met Gly Ser Val Val Arg Gly Glu Ile Asp Asn
115 120 125Val Arg Met Val Arg Gly Asp Gly Ile Glu Val Leu Asn Arg Met Phe
130 135 140Ala Asp Gly Ser Leu Asp Gly Ile Arg Val Tyr Phe Pro Asp Pro Trp145 150 155 160Pro Lys Ala Arg His Asn Lys Arg Arg Ile Ile Gln Ser Gly Pro Leu
165 170 175Asn Leu Phe Ala Lys Lys Leu Lys Pro Gly Gly Val Leu His Val Ala
180 185 190Thr Asp His Ala Asp Tyr Ala Glu Trp Ile Asn Glu Leu Val Glu Val
195 200 205Glu Pro Leu Leu Glu Tyr Lys Gly Trp Pro Trp Glu Glu Cys Pro Gln
210 215 220Leu Thr Asp Arg Gln Val Ile Thr Lys Phe Glu Gly Lys Gly Leu Glu225 230 235 240Lys Asp His Val Ile Asn Glu Tyr Leu Trp Gln Lys Val Gln Asn
245 250 255<210>153<211>948<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(925)<223>RXA00226<400>153ccgcctgcgg tgtcagcgga gcgcgcccgg cgtctgaaaa ctgcact tgg tgagaacgtg 60attgttcatg atgtcacctg ttccatcggt acggaggggc atg aac tta tcg atg 115
Met Asn Leu Ser Met
1 5ccg gcc ttc gct acc tgg gtt ctg atc cta gat ttc tca cgc acc ctc 163Pro Ala Phe Ala Thr Trp Val Leu Ile Leu Asp Phe Ser Arg Thr Leu
10 15 20atg gca gcc cac aat ctc cag ggc aaa aac gcc ctg att ttc cgc gcc 211Met Ala Ala His Asn Leu Gln Gly Lys Asn Ala Leu Ile Phe Arg Ala
25 30 35gac gcg ctc cag ccc gca agc agg gga gcc gac gtc atc atc gcg gac 259Asp Ala Leu Gln Pro Ala Ser Arg Gly Ala Asp Val Ile Ile Ala Asp
40 45 50cct gcc aga cgc gcc ggg ggc aag cgc att aca aat ccg gca cag ctc 307Pro Ala Arg Arg Ala Gly Gly Lys Arg Ile Thr Asn Pro Ala Gln Leu
55 60 65ctg cca cct ctg cct tcg ctt ctc gac gcc tgg atc aac caa cca ctc 355Leu Pro Pro Leu Pro Ser Leu Leu Asp Ala Trp Ile Asn Gln Pro Leu70 75 80 85gcc gtt aaa tgt gcc ccc ggc ctt gat ttt tcg gaa tgg cca ggt ctc 403Ala Val Lys Cys Ala Pro Gly Leu Asp Phe Ser Glu Trp Pro Gly Leu
90 95 100gtc agt att gcc agc gtt gat gga ggc gtg aaa gaa gca tgc ctc tac 451Val Ser Ile Ala Ser Val Asp Gly Gly Val Lys Glu Ala Cys Leu Tyr
105 110 115act acg gat ctg gca gat ggg gaa act cgc gaa gct atc gtg atc aaa 499Thr Thr Asp Leu Ala Asp Gly Glu Thr Arg Glu Ala Ile Val Ile Lys
120 125 130gat ggg ctc att gac cgc atc acc aac ttt gaa gac gat gcc acg gga 547Asp Gly Leu Ile Asp Arg Ile Thr Asn Phe Glu Asp Asp Ala Thr Gly
135 140 145caa gac ctt gcg gct gca cct ggt gag ttc atc atc gac cca gac ggt 595Gln Asp Leu Ala Ala Ala Pro Gly Glu Phe Ile Ile Asp Pro Asp Gly150 155 160 165gcc atc gtg cgc gcc ggg ttg gtt cgc cac tat gca gtg cgt gag cag 643Ala Ile Val Arg Ala Gly Leu Val Arg His Tyr Ala Val Arg Glu Gln
170 175 180ctg tgg atg ttg gat gag cgg atc gca tac ctt acg ggc aat cgg att 691Leu Trp Met Leu Asp Glu Arg Ile Ala Tyr Leu Thr Gly Asn Arg Ile
185 190 195cca gag ggt acc agc ggt ttt agg ttt att gaa gag gtt ccg ctg aag 739Pro Glu Gly Thr Ser Gly Phe Arg Phe Ile Glu Glu Val Pro Leu Lys
200 205 210aag ctg aaa tcg gcg atg gca gca cat gat gcg ggg gcg gtt gaa att 787Lys Leu Lys Ser Ala Met Ala Ala His Asp Ala Gly Ala Val Glu Ile
215 220 225tta gtg cgt ggt gtt gat gtt gat cct gat cag ttg cgg aaa aga ttg 835Leu Val Arg Gly Val Asp Val Asp Pro Asp Gln Leu Arg Lys Arg Leu230 235 240 245cag ctg aag ggt acc aag gcg atg tct gtg gtg atc act cga att ggc 883Gln Leu Lys Gly Thr Lys Ala Met Ser Val Val Ile Thr Arg Ile Gly
250 255 260agc cga ggg gtt gca ttg att tgt ggt cct cgc gag cgc gcc 925Ser Arg Gly Val Ala Leu Ile Cys Gly Pro Arg Glu Arg Ala
265 270 275taaagccgat gcaaataaaa ttg 948<210>154<211>275<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>154Met Asn Leu Ser Met Pro Ala Phe Ala Thr Trp Val Leu Ile Leu Asp1 5 10 15Phe Ser Arg Thr Leu Met Ala Ala His Asn Leu Gln Gly Lys Asn Ala
20 25 30Leu Ile Phe Arg Ala Asp Ala Leu Gln Pro Ala Ser Arg Gly Ala Asp
35 40 45Val Ile Ile Ala Asp Pro Ala Arg Arg Ala Gly Gly Lys Arg Ile Thr
50 55 60Asn Pro Ala Gln Leu Leu Pro Pro Leu Pro Ser Leu Leu Asp Ala Trp65 70 75 80Ile Asn Gln Pro Leu Ala Val Lys Cys Ala Pro Gly Leu Asp Phe Ser
85 90 95Glu Trp Pro Gly Leu Val Ser Ile Ala Ser Val Asp Gly Gly Val Lys
100 105 110Glu Ala Cys Leu Tyr Thr Thr Asp Leu Ala Asp Gly Glu Thr Arg Glu
115 120 125Ala Ile Val Ile Lys Asp Gly Leu Ile Asp Arg Ile Thr Asn Phe Glu
130 135 140Asp Asp Ala Thr Gly Gln Asp Leu Ala Ala Ala Pro Gly Glu Phe Ile145 150 155 160Ile Asp Pro Asp Gly Ala Ile Val Arg Ala Gly Leu Val Arg His Tyr
165 170 175Ala Val Arg Glu Gln Leu Trp Met Leu Asp Glu Arg Ile Ala Tyr Leu
180 185 190Thr Gly Asn Arg Ile Pro Glu Gly Thr Ser Gly Phe Arg Phe Ile Glu
195 200 205Glu Val Pro Leu Lys Lys Leu Lys Ser Ala Met Ala Ala His Asp Ala
210 215 220Gly Ala Val Glu Ile Leu Val Arg Gly Val Asp Val Asp Pro Asp Gln225 230 235 240Leu Arg Lys Arg Leu Gln Leu Lys Gly Thr Lys Ala Met Ser Val Val
245 250 255Ile Thr Arg Ile Gly Ser Arg Gly Val Ala Leu Ile Cys Gly Pro Arg
260 265 270Glu Arg Ala
275<210>155<211>924<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(901)<223>RXN01885<400>155gtggcgtcgc agggatgttc ctgcggcacc atttttgctg aggtggaact cacggattaa 60acacggattt ttctaaggtt aatcaagtaa ggtttacctt atg act acg aaa cct 115
Met Thr Thr Lys Pro
1 5atc atc cca gaa tca acc cac tcc gca gaa cgt gct ggt gga cat tgg 163Ile Ile Pro Glu Ser Thr His Ser Ala Glu Arg Ala Gly Gly His Trp
10 15 20atc ctt gcc agg ctt gga aag aaa gtg ctg cgc cct gga ggt cgt gaa 211Ile Leu Ala Arg Leu Gly Lys Lys Val Leu Arg Pro Gly Gly Arg Glu
25 30 35aca acg cag ttc ctg ctg gag aac ctt tct ttg acc ggt gct acc gtg 259Thr Thr Gln Phe Leu Leu Glu Asn Leu Ser Leu Thr Gly Ala Thr Val
40 45 50gtg gaa ttt gct cca gga ctt ggc gtg act gca cgt gac atc ctt ggc 307Val Glu Phe Ala Pro Gly Leu Gly Val Thr Ala Arg Asp Ile Leu Gly
55 60 65aag ggt ccg gct cgc tac atc gga gtg gat agc gac gcg gat gca tgc 355Lys Gly Pro Ala Arg Tyr Ile Gly Val Asp Ser Asp Ala Asp Ala Cys70 75 80 85gcg aat gta cgt gcg atc tta cct gct ggt cct cac gag gtg cgc aat 403Ala Asn Val Arg Ala Ile Leu Pro Ala Gly Pro His Glu Val Arg Asn
90 95 100aca aat gcc acc gat act ggc ctt gaa agc gac tcg ttt gat gtt gtc 451Thr Asn Ala Thr Asp Thr Gly Leu Glu Ser Asp Ser Phe Asp Val Val
105 110 115atc ggc gaa gcg atg ttg acc atg cag acc gat aag cac aag ttg gag 499Ile Gly Glu Ala Met Leu Thr Met Gln Thr Asp Lys His Lys Leu Glu
120 125 130ctg atg cgc gag gca gct cga att ctg aaa cca ggc ggg ctg tac ggc 547Leu Met Arg Glu Ala Ala Arg Ile Leu Lys Pro Gly Gly Leu Tyr Gly
135 140 145att cac gag ctg tcg ctg gtg cct gac aat gtc tcc act gcg gtg aaa 595Ile His Glu Leu Ser Leu Val Pro Asp Asn Val Ser Thr Ala Val Lys150 155 160 165gag gat att gct aag gcg ctg gct cgt tcc atc aaa gtc aat gcc cgc 643Glu Asp Ile Ala Lys Ala Leu Ala Arg Ser Ile Lys Val Asn Ala Arg
170 175 180ccc atc acg gtg ccg gaa tgg gct gcg ttg gcg cgt gag gca ggg ttc 691Pro Ile Thr Val Pro Glu Trp Ala Ala Leu Ala Arg Glu Ala Gly Phe
185 190 195gat gtg att aat att cgc caa gcc gac atg gcc ctt cta tcc ctc aag 739Asp Val Ile Asn Ile Arg Gln Ala Asp Met Ala Leu Leu Ser Leu Lys
200 205 210cgg aac ctg aag gat gaa ggg cta aaa ggt gtc ttc acg att gtg agg 787Arg Asn Leu Lys Asp Glu Gly Leu Lys Gly Val Phe Thr Ile Val Arg
215 220 225aac gtg att agc caa ccg gat ctg cgc aag cga gtg ctc gga atg cga 835Asn Val Ile Ser Gln Pro Asp Leu Arg Lys Arg Val Leu Gly Met Arg230 235 240 245aag act ttc acc gag cat aaa gat cac tta ggt gcg gtt ggc atc att 883Lys Thr Phe Thr Glu His Lys Asp His Leu Gly Ala Val Gly Ile Ile
250 255 260ttg cag aag aga gcc caa tagggatctg aaatggaggg gtg 924Leu Gln Lys Arg Ala Gln
265<210>156<211>267<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>156Met Thr Thr Lys Pro Ile Ile Pro Glu Ser Thr His Ser Ala Glu Arg1 5 10 15Ala Gly Gly His Trp Ile Leu Ala Arg Leu Gly Lys Lys Val Leu Arg
20 25 30Pro Gly Gly Arg Glu Thr Thr Gln Phe Leu Leu Glu Asn Leu Ser Leu
35 40 45Thr Gly Ala Thr Val Val Glu Phe Ala Pro Gly Leu Gly Val Thr Ala
50 55 60Arg Asp Ile Leu Gly Lys Gly Pro Ala Arg Tyr Ile Gly Val Asp Ser65 70 75 80Asp Ala Asp Ala Cys Ala Asn Val Arg Ala Ile Leu Pro Ala Gly Pro
85 90 95His Glu Val Arg Asn Thr Asn Ala Thr Asp Thr Gly Leu Glu Ser Asp
100 105 110Ser Phe Asp Val Val Ile Gly Glu Ala Met Leu Thr Met Gln Thr Asp
115 120 125Lys His Lys Leu Glu Leu Met Arg Glu Ala Ala Arg Ile Leu Lys Pro
130 135 140Gly Gly Leu Tyr Gly Ile His Glu Leu Ser Leu Val Pro Asp Asn Val145 150 155 160Ser Thr Ala Val Lys Glu Asp Ile Ala Lys Ala Leu Ala Arg Ser Ile
165 170 175Lys Val Asn Ala Arg Pro Ile Thr Val Pro Glu Trp Ala Ala Leu Ala
180 185 190Arg Glu Ala Gly Phe Asp Val Ile Asn Ile Arg Gln Ala Asp Met Ala
195 200 205Leu Leu Ser Leu Lys Arg Asn Leu Lys Asp Glu Gly Leu Lys Gly Val
210 215 220Phe Thr Ile Val Arg Asn Val Ile Ser Gln Pro Asp Leu Arg Lys Arg225 230 235 240Val Leu Gly Met Arg Lys Thr Phe Thr Glu His Lys Asp His Leu Gly
245 250 255Ala Val Gly Ile Ile Leu Gln Lys Arg Ala Gln
260 265<210>157<211>924<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(901)<223>FRXA01885<400>157gtggcgtcgc agggatgttc ctgcggcacc atttttgctg aggtggaact cacggattaa 60acacggattt ttctaaggtt aatcaagtaa ggtttacctt atg act acg aaa cct 115
Met Thr Thr Lys Pro
1 5atc atc cca gaa tca acc cac tcc gca gaa cgt gct ggt gga cat tgg 163Ile Ile Pro Glu Ser Thr His Ser Ala Glu Arg Ala Gly Gly His Trp
10 15 20atc ctt gcc agg ctt gga aag aaa gtg ctg cgc cct gga ggt cgt gaa 211Ile Leu Ala Arg Leu Gly Lys Lys Val Leu Arg Pro Gly Gly Arg Glu
25 30 35aca acg cag ttc ctg ctg gag aac ctt tct ttg acc ggt gct acc gtg 259Thr Thr Gln Phe Leu Leu Glu Asn Leu Ser Leu Thr Gly Ala Thr Val
40 45 50gtg gaa ttt gct cca gga ctt ggc gtg act gca cgt gac atc ctt ggc 307Val Glu Phe Ala Pro Gly Leu Gly Val Thr Ala Arg Asp Ile Leu Gly
55 60 65aag ggt ccg gct cgc tac atc gga gtg gat agc gac gcg gat gca tgc 355Lys Gly Pro Ala Arg Tyr Ile Gly Val Asp Ser Asp Ala Asp Ala Cys70 75 80 85gcg aat gta cgt gcg atc tta cct gct ggt cct cac gag gtg cgc aat 403Ala Asn Val Arg Ala Ile Leu Pro Ala Gly Pro His Glu Val Arg Asn
90 95 100aca aat gcc acc gat act ggc ctt gaa agc gac tcg ttt gat gtt gtc 451Thr Asn Ala Thr Asp Thr Gly Leu Glu Ser Asp Ser Phe Asp Val Val
105 110 115atc ggc gaa gcg atg ttg acc atg cag acc gat aag cac aag ttg gag 499Ile Gly Glu Ala Met Leu Thr Met Gln Thr Asp Lys His Lys Leu Glu
120 125 130ctg atg cgc gag gca gct cga att ctg aaa cca ggc ggg ctg tac ggc 547Leu Met Arg Glu Ala Ala Arg Ile Leu Lys Pro Gly Gly Leu Tyr Gly
135 140 145att cac gag ctg tcg ctg gtg cct gac aat gtc tcc act gcg gtg aaa 595Ile His Glu Leu Ser Leu Val Pro Asp Asn Val Ser Thr Ala Val Lys150 155 160 165gag gat att gct aag gcg ctg gct cgt tcc atc aaa gtc aat gcc cgc 643Glu Asp Ile Ala Lys Ala Leu Ala Arg Ser Ile Lys Val Asn Ala Arg
170 175 180ccc atc acg gtg ccg gaa tgg gct gcg ttg gcg cgt gag gca ggg trc 691Pro Ile Thr Val Pro Glu Trp Ala Ala Leu Ala Arg Glu Ala Gly Phe
185 190 195gat gtg att aat att cgc caa gcc gac atg gcc ctt cta tcc ctc aag 739Asp Val Ile Asn Ile Arg Gln Ala Asp Met Ala Leu Leu Ser Leu Lys
200 205 210cgg aac ctg aag gat gaa ggg cta aaa ggt gtc ttc acg att gtg agg 787Arg Asn Leu Lys Asp Glu Gly Leu Lys Gly Val Phe Thr Ile Val Arg
215 220 225aac gtg att agc caa ccg gat ctg cgc aag cga gtg ctc gga atg cga 835Asn Val Ile Ser Gln Pro Asp Leu Arg Lys Arg Val Leu Gly Met Arg230 235 240 245aag act ttc acc gag cat aaa gat cac tta ggt gcg gtt ggc atc att 883Lys Thr Phe Thr Glu His Lys Asp His Leu Gly Ala Val Gly Ile Ile
250 255 260ttg cag aag aga gcc caa tagggatctg aaatggaggg gtg 924Leu Gln Lys Arg Ala Gln
265<210>158<211>267<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>158Met Thr Thr Lys Pro Ile Ile Pro Glu Ser Thr His Ser Ala Glu Arg1 5 10 15Ala Gly Gly His Trp Ile Leu Ala Arg Leu Gly Lys Lys Val Leu Arg
20 25 30Pro Gly Gly Arg Glu Thr Thr Gln Phe Leu Leu Glu Asn Leu Ser Leu
35 40 45Thr Gly Ala Thr Val Val Glu Phe Ala Pro Gly Leu Gly Val Thr Ala
50 55 60Arg Asp Ile Leu Gly Lys Gly Pro Ala Arg Tyr Ile Gly Val Asp Ser65 70 75 80Asp Ala Asp Ala Cys Ala Asn Val Arg Ala Ile Leu Pro Ala Gly Pro
85 90 95His Glu Val Arg Asn Thr Asn Ala Thr Asp Thr Gly Leu Glu Ser Asp
100 105 110Ser Phe Asp Val Val Ile Gly Glu Ala Met Leu Thr Met Gln Thr Asp
115 120 125Lys His Lys Leu Glu Leu Met Arg Glu Ala Ala Arg Ile Leu Lys Pro
130 135 140Gly Gly Leu Tyr Gly Ile His Glu Leu Ser Leu Val Pro Asp Asn Val145 150 155 160Ser Thr Ala Val Lys Glu Asp Ile Ala Lys Ala Leu Ala Arg Ser Ile
165 170 175Lys Val Asn Ala Arg Pro Ile Thr Val Pro Glu Trp Ala Ala Leu Ala
180 185 190Arg Glu Ala Gly Phe Asp Val Ile Asn Ile Arg Gln Ala Asp Met Ala
195 200 205Leu Leu Ser Leu Lys Arg Asn Leu Lys Asp Glu Gly Leu Lys Gly Val
210 215 220Phe Thr Ile Val Arg Asn Val Ile Ser Gln Pro Asp Leu Arg Lys Arg225 230 235 240Val Leu Gly Met Arg Lys Thr Phe Thr Glu His Lys Asp His Leu Gly
245 250 255Ala Val Gly Ile Ile Leu Gln Lys Arg Ala Gln
260 265<210>159<211>894<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(871)<223>RXA02592<400>159aaacaccacc attgtttcct ccatcgacga tgccatcacc accattttgc gatggatgaa 60cggcgaagac atccgcgacc tcaactggac ccgcgcataa atg gcc tca ttt ccg 115
Met Ala Ser Phe Pro
1 5gag ctt ccg gct ctt cgt cgc ttg gct acc ttg ggc agg tcg tgg ggt 163Glu Leu Pro Ala Leu Arg Arg Leu Ala Thr Leu Gly Arg Ser Trp Gly
10 15 20tta ctg tct gat ttc aaa tac gaa caa acc cga cct gac atc ttt tac 211Leu Leu Ser Asp Phe Lys Tyr Glu Gln Thr Arg Pro Asp Ile Phe Tyr
25 30 35gga aac ctg gcc ctc gat acc tcg agt ctg gtg gcg gct ttg tct gaa 259Gly Asn Leu Ala Leu Asp Thr Ser Ser Leu Val Ala Ala Leu Ser Glu
40 45 50gat att tct ggc gcc gga tta aat gac ctg aaa gtt ctc gac gtc ggc 307Asp Ile Ser Gly Ala Gly Leu Asn Asp Leu Lys Val Leu Asp Val Gly
55 60 65ggc gga ccc gga tac ttc gcc gaa gcc ttt gag aca ctg ggc gcc acc 355Gly Gly Pro Gly Tyr Phe Ala Glu Ala Phe Glu Thr Leu Gly Ala Thr70 75 80 85tac ttc tcc gtc gaa ccc gac gtt ggc gaa atg tcc gca gct ggc atc 403Tyr Phe Ser Val Glu Pro Asp Val Gly Glu Met Ser Ala Ala Gly Ile
90 95 100gac gtc cac gga tca gtc cgc gga tcc ggc ctc gac ctg ccg ttt ctt 451Asp Val His Gly Ser Val Arg Gly Ser Gly Leu Asp Leu Pro Phe Leu
105 110 115ccc gat tcc ttt gac gtg gtg tac tcc tcc aac gtt gca gaa cat gtc 499Pro Asp Ser Phe Asp Val Val Tyr Ser Ser Asn Val Ala Glu His Val
120 125 130tcc gca ccg tgg gaa ttg gga gaa gaa atg ctc cgc gtc acc cgc agc 547Ser Ala Pro Trp Glu Leu Gly Glu Glu Met Leu Arg Val Thr Arg Ser
135 140 145ggc ggc ctg gca atc ctg agc tac acc att tgg tta ggg ccc ttc ggc 595Gly Gly Leu Ala Ile Leu Ser Tyr Thr Ile Trp Leu Gly Pro Phe Gly150 155 160 165ggc cat gaa acc gga ctg tgg gaa cac tac gtt ggc gga gaa ttt gcc 643Gly His Glu Thr Gly Leu Trp Glu His Tyr Val Gly Gly Glu Phe Ala
170 175 180cgc gat cgc tac acg aag aaa cac ggg cac ccg cct aag aac gtt ttc 691Arg Asp Arg Tyr Thr Lys Lys His Gly His Pro Pro Lys Asn Val Phe
185 190 195ggg gag tca ctg ttt aat gtg tcc tgc cgg gag ggg ctg gaa tgg gga 739Gly Glu Ser Leu Phe Asn Val Ser Cys Arg Glu Gly Leu Glu Trp Gly
200 205 210gcc tcc gtg ggc aat gcg gaa ttg gtt gcc gct ttt ccc cgc tac cac 787Ala Ser Val Gly Asn Ala Glu Leu Val Ala Ala Phe Pro Arg Tyr His
215 220 225ccg tat tgg gtc tgg tgg atg gtt aaa gtc cca gtg ctc cga gaa ttc 835Pro Tyr Trp Val Trp Trp Met Val Lys Val Pro Val Leu Arg Glu Phe230 235 240 245gcg gta agt aac ttg gtg ttg gtg ttt aaa aag cac tgaggttttg 881Ala Val Ser Asn Leu Val Leu Val Phe Lys Lys His
250 255aggaattcat cgc 894<210> 160<211>257<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>160Met Ala Ser Phe Pro Glu Leu Pro Ala Leu Arg Arg Leu Ala Thr Leu1 5 10 15Gly Arg Ser Trp Gly Leu Leu Ser Asp Phe Lys Tyr Glu Gln Thr Arg
20 25 30Pro Asp Ile Phe Tyr Gly Asn Leu Ala Leu Asp Thr Ser Ser Leu Val
35 40 45Ala Ala Leu Ser Glu Asp Ile Ser Gly Ala Gly Leu Asn Asp Leu Lys
50 55 60Val Leu Asp Val Gly Gly Gly Pro Gly Tyr Phe Ala Glu Ala Phe Glu65 70 75 80Thr Leu Gly Ala Thr Tyr Phe Ser Val Glu Pro Asp Val Gly Glu Met
85 90 95Ser Ala Ala Gly Ile Asp Val His Gly Ser Val Arg Gly Ser Gly Leu
100 105 110Asp Leu Pro Phe Leu Pro Asp Ser Phe Asp Val Val Tyr Ser Ser Asn
115 120 125Val Ala Glu His Val Ser Ala Pro Trp Glu Leu Gly Glu Glu Met Leu
130 135 140Arg Val Thr Arg Ser Gly Gly Leu Ala Ile Leu Ser Tyr Thr Ile Trp145 150 155 160Leu Gly Pro Phe Gly Gly His Glu Thr Gly Leu Trp Glu His Tyr Val
165 170 175Gly Gly Glu Phe Ala Arg Asp Arg Tyr Thr Lys Lys His Gly His Pro
180 185 190Pro Lys Asn Val Phe Gly Glu Ser Leu Phe Asn Val Ser Cys Arg Glu
195 200 205Gly Leu Glu Trp Gly Ala Ser Val Gly Asn Ala Glu Leu Val Ala Ala
210 215 220Phe Pro Arg Tyr His Pro Tyr Trp Val Trp Trp Met Val Lys Val Pro225 230 235 240Val Leu Arg Glu Phe Ala Val Ser Asn Leu Val Leu Val Phe Lys Lys
245 250 255His<210>161<211>720<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(697)<223>RXN01795<400>161agaccatatt gaagacctcg aagctgttga gcctggctac atcgtcaagc ctcgcctgta 60caacttcgct gaatacggtg tcccacaatt ccgcgaacgt gtg ctc att gtt ggc 115
Val Leu Ile Val Gly
1 5att cgc cgt gac acc ggc ttt gat ttc aag cac cca gct cct acc cat 163Ile Arg Arg Asp Thr Gly Phe Asp Phe Lys His Pro Ala Pro Thr His
10 15 20ggc cct cgc ggt gac atg ccg tat aag act gcc ggc gaa gcg ctc aaa 211Gly Pro Arg Gly Asp Met Pro Tyr Lys Thr Ala Gly Glu Ala Leu Lys
25 30 35ggc gtg aag gat gtc ccc aca aac aac aac cac atg aag atc atg cct 259Gly Val Lys Asp Val Pro Thr Asn Asn Asn His Met Lys Ile Met Pro
40 45 50cgc acc gtt gaa gtg ctt aag cgc atc cct gag ggc gaa aac ttc acc 307Arg Thr Val Glu Val Leu Lys Arg Ile Pro Glu Gly Glu Asn Phe Thr
55 60 65gcg atc ccc aaa gat gac ccc tac tac gtc aag ggc atg att agt cac 355Ala Ile Pro Lys Asp Asp Pro Tyr Tyr Val Lys Gly Met Ile Ser His70 75 80 85gtt tac cgt cgc ttg cac cgt gat gag cca tcc aaa acc ctt atc gcc 403Val Tyr Arg Arg Leu His Arg Asp Glu Pro Ser Lys Thr Leu Ile Ala
90 95 100ggt ggc ggc ggg ggt aca tgg gga tac cat tat gaa aaa aat cga gca 451Gly Gly Gly Gly Gly Thr Trp Gly Tyr His Tyr Glu Lys Asn Arg Ala
105 110 115ttg acc aac cgc gag cgg gct aga att caa tcg ttc ccc gat gac ttt 499Leu Thr Asn Arg Glu Arg Ala Arg Ile Gln Ser Phe Pro Asp Asp Phe
120 125 130gag ttt ttg gga tca aac acc gaa gtc cgc cgc caa atc ggt aat gct 547Glu Phe Leu Gly Ser Asn Thr Glu Val Arg Arg Gln Ile Gly Asn Ala
135 140 145gtt cct cct gta ggt atg cac gct gtg ggt gag cga ctg atg aac ctg 595Val Pro Pro Val Gly Met His Ala Val Gly Glu Arg Leu Met Asn Leu150 155 160 165tac acc ggg aat tac act ccc gtc gat cta gag gaa cag cac gcg tac 643Tyr Thr Gly Asn Tyr Thr Pro Val Asp Leu Glu Glu Gln His Ala Tyr
170 175 180ctg cag acg ctc tcc att aag gaa cgt ctc gcg ctg gct gat cag gaa 691Leu Gln Thr Leu Ser Ile Lys Glu Arg Leu Ala Leu Ala Asp Gln Glu
185 190 195gct gat taagtagata tatgaagccc acc 720Ala Asp<210>162<211>199<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>162Val Leu Ile Val Gly Ile Arg Arg Asp Thr Gly Phe Asp Phe Lys His1 5 10 15Pro Ala Pro Thr His Gly Pro Arg Gly Asp Met Pro Tyr Lys Thr Ala
20 25 30Gly Glu Ala Leu Lys Gly Val Lys Asp Val Pro Thr Asn Asn Asn His
35 40 45Met Lys Ile Met Pro Arg Thr Val Glu Val Leu Lys Arg Ile Pro Glu
50 55 60Gly Glu Asn Phe Thr Ala Ile Pro Lys Asp Asp Pro Tyr Tyr Val Lys65 70 75 80Gly Met Ile Ser His Val Tyr Arg Arg Leu His Arg Asp Glu Pro Ser
85 90 95Lys Thr Leu Ile Ala Gly Gly Gly Gly Gly Thr Trp Gly Tyr His Tyr
100 105 110Glu Lys Asn Arg Ala Leu Thr Asn Arg Glu Arg Ala Arg Ile Gln Ser
115 120 125Phe Pro Asp Asp Phe Glu Phe Leu Gly Ser Asn Thr Glu Val Arg Arg
130 135 140Gln Ile Gly Asn Ala Val Pro Pro Val Gly Met His Ala Val Gly Glu145 150 155 160Arg Leu Met Asn Leu Tyr Thr Gly Asn Tyr Thr Pro Val Asp Leu Glu
165 170 175Glu Gln His Ala Tyr Leu Gln Thr Leu Ser Ile Lys Glu Arg Leu Ala
180 185 190Leu Ala Asp Gln Glu Ala Asp
195<210>163<211>535<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(535)<223>FRXA01795<400>163agaccatatt gaagacctcg aagctgttga gcctggctac atcgtcaagc ctcgcctgta 60caacttcgct gaatacggtg tcccacaatt ccgcgaacgt gtg ctc att gtt ggc 115
Val Leu Ile Val Gly
1 5att cgc cgt gac acc ggc ttt gat ttc aag cac cca gct cct acc cat 163Ile Arg Arg Asp Thr Gly Phe Asp Phe Lys His Pro Ala Pro Thr His
10 15 20ggc cct cgc ggt gac atg ccg tat aag act gcc ggc gaa gcg ctc aaa 211Gly Pro Arg Gly Asp Met Pro Tyr Lys Thr Ala Gly Glu Ala Leu Lys
25 30 35ggc gtg aag gat gtc ccc aca aac aac aac cac atg aag atc atg cct 259Gly Val Lys Asp Val Pro Thr Asn Asn Asn His Met Lys Ile Met Pro
40 45 50cgc acc gtt gaa gtg ctt aag cgc atc cct gag ggc gaa aac ttc acc 307Arg Thr Val Glu Val Leu Lys Arg Ile Pro Glu Gly Glu Asn Phe Thr
55 60 65gcg atc ccc aaa gat gac ccc tac tac gtc aag ggc atg att agt cac 355Ala Ile Pro Lys Asp Asp Pro Tyr Tyr Val Lys Gly Met Ile Ser His70 75 80 85gtt tac cgt cgc ttg cac cgt gat gag cca tcc aaa acc ctt atc gcc 403Val Tyr Arg Arg Leu His Arg Asp Glu Pro Ser Lys Thr Leu Ile Ala
90 95 100ggt ggc ggc ggg ggt aca tgg gga tac cat tat gaa aaa aat cga gca 451Gly Gly Gly Gly Gly Thr Trp Gly Tyr His Tyr Glu Lys Asn Arg Ala
105 110 115ttg acc aac cgc gag cgg gct aga att caa tcg ttc ccc gat gac ttt 499Leu Thr Asn Arg Glu Arg Ala Arg Ile Gln Ser Phe Pro Asp Asp Phe
120 125 130gag ttt ttg gga tca aac acc caa gtc cgc cgc caa 535Glu Phe Leu Gly Ser Asn Thr Gln Val Arg Arg Gln
135 140 145<210>164<211>145<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>164Val Leu Ile Val Gly Ile Arg Arg Asp Thr Gly Phe Asp Phe Lys His1 5 10 15Pro Ala Pro Thr His Gly Pro Arg Gly Asp Met Pro Tyr Lys Thr Ala
20 25 30Gly Glu Ala Leu Lys Gly Val Lys Asp Val Pro Thr Asn Asn Asn His
35 40 45Met Lys Ile Met Pro Arg Thr Val Glu Val Leu Lys Arg Ile Pro Glu
50 55 60Gly Glu Asn Phe Thr Ala Ile Pro Lys Asp Asp Pro Tyr Tyr Val Lys65 70 75 80Gly Met Ile Ser His Val Tyr Arg Arg Leu His Arg Asp Glu Pro Ser
85 90 95Lys Thr Leu Ile Ala Gly Gly Gly Gly Gly Thr Trp Gly Tyr His Tyr
100 105 110Glu Lys Asn Arg Ala Leu Thr Asn Arg Glu Arg Ala Arg Ile Gln Ser
115 120 125Phe Pro Asp Asp Phe Glu Phe Leu Gly Ser Asn Thr Gln Val Arg Arg
130 135 140Gln145<210>165<211>1614<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1591)<223>RXA01214<400>165gttgatatga tgtggggtgt ttatgaaaat ttgtttgagg gagtgaaggc gcatgttgtt 60gccagagttg aatcgtcgga cttttttcaa aggggccggg gtg ctg gca gca acg 115
Val Leu Ala Ala Thr
1 5gtg gtg ggt gcg cag gtg ctg gtg gcg tgt tcc tca gat gat gtg cgt 163Val Val Gly Ala Gln Val Leu Val Ala Cys Ser Ser Asp Asp Val Arg
10 15 20ggt tat ggg gga gag ccg cgg acg ttg cct att cca cca gca gat tta 211Gly Tyr Gly Gly Glu Pro Arg Thr Leu Pro Ile Pro Pro Ala Asp Leu
25 30 35ggt acg cgt gag gga tct agc gtg cac ttt gcc ctg gag gct cag act 259Gly Thr Arg Glu Gly Ser Ser Val His Phe Ala Leu Glu Ala Gln Thr
40 45 50ggg gag agt cag att ttg ccg gat gtc aca acg aag acg tgg ggt ttc 307Gly Glu Ser Gln Ile Leu Pro Asp Val Thr Thr Lys Thr Trp Gly Phe
55 60 65aat ggc act cat ttg ggg ccg acg ttg gtg gtg aag aaa ggt gat gac 355Asn Gly Thr His Leu Gly Pro Thr Leu Val Val Lys Lys Gly Asp Asp70 75 80 85gtc cac gtt gat gtg ata aac aat ttg gat gaa atg acc act gtg cac 403Val His Val Asp Val Ile Asn Asn Leu Asp Glu Met Thr Thr Val His
90 95 100tgg cat ggc atg aag ttg ccg gcg att gct gat ggt ggt ccg cac tca 451Trp His Gly Met Lys Leu Pro Ala Ile Ala Asp Gly Gly Pro His Ser
105 110 115ccg atc ggg cct ggg cag acg tgg tca cca acg tgg act gtg gcc aat 499Pro Ile Gly Pro Gly Gln Thr Trp Ser Pro Thr Trp Thr Val Ala Asn
120 125 130gat gca gcc act ttg tgg tac cac ccg cac act cat ggc ctg aca ggt 547Asp Ala Ala Thr Leu Trp Tyr His Pro His Thr His Gly Leu Thr Gly
135 140 145ttg cat gcg tac cgt ggt ttg gcg ggg atg atc att gtg gaa gat gaa 595Leu His Ala Tyr Arg Gly Leu Ala Gly Met Ile Ile Val Glu Asp Glu150 155 160 165gca aca gac aag ctg gat ctg cca cgc gag tac ggt gtg gac gat att 643Ala Thr Asp Lys Leu Asp Leu Pro Arg Glu Tyr Gly Val Asp Asp Ile
170 175 180ccg ctg gtt tta atg gat cac cgc ttc tta gaa gac ggt tcc ctt gat 691Pro Leu Val Leu Met Asp His Arg Phe Leu Glu Asp Gly Ser Leu Asp
185 190 195gag gaa gac ctc ccc gat ctt ggg ctg ttg ggc gat acc ccc act gcc 739Glu Glu Asp Leu Pro Asp Leu Gly Leu Leu Gly Asp Thr Pro Thr Ala
200 205 210aat ggc att acc aat gcg cac ttt gat gcc acc acg cgc cgg gtt cgg 787Asn Gly Ile Thr Asn Ala His Phe Asp Ala Thr Thr Arg Arg Val Arg
215 220 225ttc cgc gtg ctc aac ggc tcc aat atg cgg ttc tat aac ttg gcg ttt 835Phe Arg Val Leu Asn Gly Ser Asn Met Arg Phe Tyr Asn Leu Ala Phe230 235 240 245tca gac acg cgc acc ttc caa gtc att gcc agc gat tcc ggt ttg ctg 883Ser Asp Thr Arg Thr Phe Gln Val Ile Ala Ser Asp Ser Gly Leu Leu
250 255 260gat gaa cct caa gac cgc acc acc ttg gct att ggc cca ggc gag cgg 931Asp Glu Pro Gln Asp Arg Thr Thr Leu Ala Ile Gly Pro Gly Glu Arg
265 270 275tgg gaa atc gtc gtg gag cta gag ccc ggc gag gac gtc acc ttg gaa 979Trp Glu Ile Val Val Glu Leu Glu Pro Gly Glu Asp Val Thr Leu Glu
280 285 290tct gta ggt ttt gag gac aac tac ggc gtc cct gat gat gag ttc gtg 1027Ser Val Gly Phe Glu Asp Asn Tyr Gly Val Pro Asp Asp Glu Phe Val
295 300 305ccc gat ttc ggc atg tca gat tcc ttc cag ctg ctc acc atc acc ggc 1075Pro Asp Phe Gly Met Ser Asp Ser Phe Gln Leu Leu Thr Ile Thr Gly310 315 320 325cct tcc gat gat gct gcg caa gca cct gct ttg ccg ggc gtg ctg gtg 1123Pro Ser Asp Asp Ala Ala Gln Ala Pro Ala Leu Pro Gly Val Leu Val
330 335 340aaa tcc acc gaa cct gac gtc atc gat gcc act gaa cgc acc ttc atc 1171Lys Ser Thr Glu Pro Asp Val Ile Asp Ala Thr Glu Arg Thr Phe Ile
345 350 355atg aac acc ttc tcc atc aac gat cta cag atg gac atg cag cgc gtt 1219Met Asn Thr Phe Ser Ile Asn Asp Leu Gln Met Asp Met Gln Arg Val
360 365 370gac gtg gtg att gac cat gac cag cca gaa gtg tgg att gtc acc aac 1267Asp Val Val Ile Asp His Asp Gln Pro Glu Val Trp Ile Val Thr Asn
375 380 385gac aac tcc gac tgg ccc cac aac ttc cat gtc cac gac gcc cgg ttt 1315Asp Asn Ser Asp Trp Pro His Asn Phe His Val His Asp Ala Arg Phe390 395 400 405aag gtg ctg aaa ttt gaa ggc acc gac gta gag ctc ttc aac gac ggc 1363Lys Val Leu Lys Phe Glu Gly Thr Asp Val Glu Leu Phe Asn Asp Gly
410 415 420tgg aaa gac acc gtc ggc ctg cca ccg gga gca acc gca act tta gcc 1411Trp Lys Asp Thr Val Gly Leu Pro Pro Gly Ala Thr Ala Thr Leu Ala
425 430 435gtg gaa ttt ggc cac tac cca gac ccg caa tgg ccc tac atg tat cac 1459Val Glu Phe Gly His Tyr Pro Asp Pro Gln Trp Pro Tyr Met Tyr His
440 445 450tgc cac atg ctc tac cac gag gat caa ggc atg atg ggg cag ttc gtc 1507Cys His Met Leu Tyr His Glu Asp Gln Gly Met Met Gly Gln Phe Val
455 460 465atc gtg gag cca ggc gac gag ccg gcg gcg gtg ctg ggg tcg ggc acg 1555Ile Val Glu Pro Gly Asp Glu Pro Ala Ala Val Leu Gly Ser Gly Thr470 475 480 485ggc tcc agc att gac tcc gcc ggc gga cat gcg cac taggggcgtg 1601Gly Ser Ser Ile Asp Ser Ala Gly Gly His Ala His
490 495gggcggcgtc gat 1614<210>166<211>497<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>166Val Leu Ala Ala Thr Val Val Gly Ala Gln Val Leu Val Ala Cys Ser1 5 10 15Ser Asp Asp Val Arg Gly Tyr Gly Gly Glu Pro Arg Thr Leu Pro Ile
20 25 30Pro Pro Ala Asp Leu Gly Thr Arg Glu Gly Ser Ser Val His Phe Ala
35 40 45Leu Glu Ala Gln Thr Gly Glu Ser Gln Ile Leu Pro Asp Val Thr Thr
50 55 60Lys Thr Trp Gly Phe Asn Gly Thr His Leu Gly Pro Thr Leu Val Val65 70 75 80Lys Lys Gly Asp Asp Val His Val Asp Val Ile Asn Asn Leu Asp Glu
85 90 95Met Thr Thr Val His Trp His Gly Met Lys Leu Pro Ala Ile Ala Asp
100 105 110Gly Gly Pro His Ser Pro Ile Gly Pro Gly Gln Thr Trp Ser Pro Thr
115 120 125Trp Thr Val Ala Asn Asp Ala Ala Thr Leu Trp Tyr His Pro His Thr
130 135 140His Gly Leu Thr Gly Leu His Ala Tyr Arg Gly Leu Ala Gly Met Ile145 150 155 160Ile Val Glu Asp Glu Ala Thr Asp Lys Leu Asp Leu Pro Arg Glu Tyr
165 170 175Gly Val Asp Asp Ile Pro Leu Val Leu Met Asp His Arg Phe Leu Glu
180 185 190Asp Gly Ser Leu Asp Glu Glu Asp Leu Pro Asp Leu Gly Leu Leu Gly
195 200 205Asp Thr Pro Thr Ala Asn Gly Ile Thr Asn Ala His Phe Asp Ala Thr
210 215 220Thr Arg Arg Val Arg Phe Arg Val Leu Asn Gly Ser Asn Met Arg Phe225 230 235 240Tyr Asn Leu Ala Phe Ser Asp Thr Arg Thr Phe Gln Val Ile Ala Ser
245 250 255Asp Ser Gly Leu Leu Asp Glu Pro Gln Asp Arg Thr Thr Leu Ala Ile
260 265 270Gly Pro Gly Glu Arg Trp Glu Ile Val Val Glu Leu Glu Pro Gly Glu
275 280 285Asp Val Thr Leu Glu Ser Val Gly Phe Glu Asp Asn Tyr Gly Val Pro
290 295 300Asp Asp Glu Phe Val Pro Asp Phe Gly Met Ser Asp Ser Phe Gln Leu305 310 315 320Leu Thr Ile Thr Gly Pro Ser Asp Asp Ala Ala Gln Ala Pro Ala Leu
325 330 335Pro Gly Val Leu Val Lys Ser Thr Glu Pro Asp Val Ile Asp Ala Thr
340 345 350Glu Arg Thr Phe Ile Met Asn Thr Phe Ser Ile Asn Asp Leu Gln Met
355 360 365Asp Met Gln Arg Val Asp Val Val Ile Asp His Asp Gln Pro Glu Val
370 375 380Trp Ile Val Thr Asn Asp Asn Ser Asp Trp Pro His Asn Phe His Val385 390 395 400His Asp Ala Arg Phe Lys Val Leu Lys Phe Glu Gly Thr Asp Val Glu
405 410 415Leu Phe Asn Asp Gly Trp Lys Asp Thr Val Gly Leu Pro Pro Gly Ala
420 425 430Thr Ala Thr Leu Ala Val Glu Phe Gly His Tyr Pro Asp Pro Gln Trp
435 440 445Pro Tyr Met Tyr His Cys His Met Leu Tyr His Glu Asp Gln Gly Met
450 455 460Met Gly Gln Phe Val Ile Val Glu Pro Gly Asp Glu Pro Ala Ala Val465 470 475 480Leu Gly Ser Gly Thr Gly Ser Ser Ile Asp Ser Ala Gly Gly His Ala
485 490 495His<210>167<211>588<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(588)<223>RXA01250<400>167ctg gat atc ggc ggc atc gaa gcc aag acg tgg gga tac gtc tct gac 48Leu Asp Ile Gly Gly Ile Glu Ala Lys Thr Trp Gly Tyr Val Ser Asp1 5 10 15acc ggg gat gcg gcc att gag gcc acc gcc ggc gac gtc ctc cag gtc 96Thr Gly Asp Ala Ala Ile Glu Ala Thr Ala Gly Asp Val Leu Gln Val
20 25 30gat atc acc aat gac ctg cct gag agc acc tcc atc cac tgg cat ggc 144Asp Ile Thr Asn Asp Leu Pro Glu Ser Thr Ser Ile His Trp His Gly
35 40 45atc gca ctc cac aac gca gcc gac ggt gtg ccc ggc atg acc cag gac 192Ile Ala Leu His Asn Ala Ala Asp Gly Val Pro Gly Met Thr Gln Asp
50 55 60ccc att gaa cct ggc gag tct ttc tcc tat gtt ttt gaa gtc ccc cac 240Pro Ile Glu Pro Gly Glu Ser Phe Ser Tyr Val Phe Glu Val Pro His65 70 75 80ggt ggc acc tac ttc tac cat tcc cac acc ggc ctg cag ctt gat cgc 288Gly Gly Thr Tyr Phe Tyr His Ser His Thr Gly Leu Gln Leu Asp Arg
85 90 95ggc ctc cac gcc cca ctg atc atc cgt gac ccg caa gac gct gag gac 336Gly Leu His Ala Pro Leu Ile Ile Arg Asp Pro Gln Asp Ala Glu Asp
100 105 110cag gac gtc gag tgg acc atc gtg ctc gac gac tgg gtc gat ggc att 384Gln Asp Val Glu Trp Thr Ile Val Leu Asp Asp Trp Val Asp Gly Ile
115 120 125cag ggc act ccc gac gat gag ctc gac aag ctc acc gga atg ggt tcg 432Gln Gly Thr Pro Asp Asp Glu Leu Asp Lys Leu Thr Gly Met Gly Ser
130 135 140ggc gac cat aac ggg agg atg gga atg gga ggt cac ggc cag atg atg 480Gly Asp His Asn Gly Arg Met Gly Met Gly Gly His Gly Gln Met Met145 150 155 160cac ggc acc ccg gac cgg gta ctg ggc ggg gat gtc ggc gat gtg atg 528His Gly Thr Pro Asp Arg Val Leu Gly Gly Asp Val Gly Asp Val Met
165 170 175tat ccg cac tac ctc atc aac gga cgt atc ccc cgt gct cac cgg acc 576Tyr Pro His Tyr Leu Ile Asn Gly Arg Ile Pro Arg Ala His Arg Thr
180 185 190ttc gag gct cgc 588Phe Glu Ala Arg
195<210>168<211>196<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>168Leu Asp Ile Gly Gly Ile Glu Ala Lys Thr Trp Gly Tyr Val Ser Asp1 5 10 15Thr Gly Asp Ala Ala Ile Glu Ala Thr Ala Gly Asp Val Leu Gln Val
20 25 30Asp Ile Thr Asn Asp Leu Pro Glu Ser Thr Ser Ile His Trp His Gly
35 40 45Ile Ala Leu His Asn Ala Ala Asp Gly Val Pro Gly Met Thr Gln Asp
50 55 60Pro Ile Glu Pro Gly Glu Ser Phe Ser Tyr Val Phe Glu Val Pro His65 70 75 80Gly Gly Thr Tyr Phe Tyr His Ser His Thr Gly Leu Gln Leu Asp Arg
85 90 95Gly Leu His Ala Pro Leu Ile Ile Arg Asp Pro Gln Asp Ala Glu Asp
100 105 110Gln Asp Val Glu Trp Thr Ile Val Leu Asp Asp Trp Val Asp Gly Ile
115 120 125Gln Gly Thr Pro Asp Asp Glu Leu Asp Lys Leu Thr Gly Met Gly Ser
130 135 140Gly Asp His Asn Gly Arg Met Gly Met Gly Gly His Gly Gln Met Met145 150 155 160His Gly Thr Pro Asp Arg Val Leu Gly Gly Asp Val Gly Asp Val Met
165 170 175Tyr Pro His Tyr Leu Ile Asn Gly Arg Ile Pro Arg Ala His Arg Thr
180 185 190Phe Glu Ala Arg
195<210>169<211>744<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(721)<223>RXA02477<400>169cgagcagggc tgtttgaaaa gctgtaaatg acatgaccta aatgattgta ctgactggca 60ctttaggtca tatgtcacac cgagtggaat aataaagctt atg cct ttg cgt aat 115
Met Pro Leu Arg Asn
1 5gtt gat aga act ccg ccc gca gta tgg gaa gca ttg ctt gcc gga aac 163Val Asp Arg Thr Pro Pro Ala Val Trp Glu Ala Leu Leu Ala Gly Asn
10 15 20gaa aga ttc atc agt ttc aac gaa gat cga cca aac cag gac gcc ccg 211Glu Arg Phe Ile Ser Phe Asn Glu Asp Arg Pro Asn Gln Asp Ala Pro
25 30 35cgc aga aga gaa ctt cgc aat gga caa acg cct gca gct gtt gtt att 259Arg Arg Arg Glu Leu Arg Asn Gly Gln Thr Pro Ala Ala Val Val Ile
40 45 50tcc tgt tca gat tct cga gtg cca gtt gag att att ttt gac gtc ggt 307Ser Cys Ser Asp Ser Arg Val Pro Val Glu Ile Ile Phe Asp Val Gly
55 60 65ctc ggt gac ctc ttt gtt gtc cgt act gcc gga gaa atc ctc gac caa 355Leu Gly Asp Leu Phe Val Val Arg Thr Ala Gly Glu Ile Leu Asp Gln70 75 80 85gca gtg ctt gcg tcc atc gaa tac gcc act gaa tcc atc ggc gtt cca 403Ala Val Leu Ala Ser Ile Glu Tyr Ala Thr Glu Ser Ile Gly Val Pro
90 95 100ttg gtt atc gtc atg ggc cac gaa tcc tgt ggt gca gtt gca gca act 451Leu Val Ile Val Met Gly His Glu Ser Cys Gly Ala Val Ala Ala Thr
105 110 115gca gca gca ctt gaa ggc ggt gca ctt ccc gga ggc tac caa cga gtt 499Ala Ala Ala Leu Glu Gly Gly Ala Leu Pro Gly Gly Tyr Gln Arg Val
120 125 130ttg gtt gaa aag gtt gca cca tcc att cta gaa gcc aag gca gag ggc 547Leu Val Glu Lys Val Ala Pro Ser Ile Leu Glu Ala Lys Ala Glu Gly
135 140 145ctg agc tcc atc aag gaa ttc gag gaa cac cac gtt gtg gca acg gta 595Leu Ser Ser Ile Lys Glu Phe Glu Glu His His Val Val Ala Thr Val150 155 160 165aac caa ctg ttg tcc cgt tct cca gag att cat cag aag gtc gaa acc 643Asn Gln Leu Leu Ser Arg Ser Pro Glu Ile His Gln Lys Val Glu Thr
170 175 180ggt gag ttg gga atc att ggt ttg cgc tac cga ctc tct gac ggt cgt 691Gly Glu Leu Gly Ile Ile Gly Leu Arg Tyr Arg Leu Ser Asp Gly Arg
185 190 195act gaa cct gta att agc aag aac gtg ggt tagttttcgg tctgagattg 741Thr Glu Pro Val Ile Ser Lys Asn Val Gly
200 205cct 744<210>170<211>207<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>170Met Pro Leu Arg Asn Val Asp Arg Thr Pro Pro Ala Val Trp Glu Ala1 5 10 15Leu Leu Ala Gly Asn Glu Arg Phe Ile Ser Phe Asn Glu Asp Arg Pro
20 25 30Asn Gln Asp Ala Pro Arg Arg Arg Glu Leu Arg Asn Gly Gln Thr Pro
35 40 45Ala Ala Val Val Ile Ser Cys Ser Asp Ser Arg Val Pro Val Glu Ile
50 55 60Ile Phe Asp Val Gly Leu Gly Asp Leu Phe Val Val Arg Thr Ala Gly65 70 75 80Glu Ile Leu Asp Gln Ala Val Leu Ala Ser Ile Glu Tyr Ala Thr Glu
85 90 95Ser Ile Gly Val Pro Leu Val Ile Val Met Gly His Glu Ser Cys Gly
100 105 110Ala Val Ala Ala Thr Ala Ala Ala Leu Glu Gly Gly Ala Leu Pro Gly
115 120 125Gly Tyr Gln Arg Val Leu Val Glu Lys Val Ala Pro Ser Ile Leu Glu
130 135 140Ala Lys Ala Glu Gly Leu Ser Ser Ile Lys Glu Phe Glu Glu His His145 150 155 160Val Val Ala Thr Val Asn Gln Leu Leu Ser Arg Ser Pro Glu Ile His
165 170 175Gln Lys Val Glu Thr Gly Glu Leu Gly Ile Ile Gly Leu Arg Tyr Arg
180 185 190Leu Ser Asp Gly Arg Thr Glu Pro Val Ile Ser Lys Asn Val Gly
195 200 205<210>171<211>618<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(595)<223>RXN00833<400>171agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct 60tttctagcac caaaacaaaa ctctccctag tatggggtcc atg gct aaa aca cat 115
Met Ala Lys Thr His
1 5ttt caa ggc aac gaa act gct acc tcc ggc gaa ctg cca cag gtc ggc 163Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu Leu Pro Gln Val Gly
10 15 20gac aac ctc gca gag ttc aac ctc gtc aac acc gaa ctg ggc gag gtc 211Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr Glu Leu Gly Glu Val
25 30 35tcc tca aag gac ttc cag ggc cgc aag ctt gtc ctg aac atc ttc cca 259Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val Leu Asn Ile Phe Pro
40 45 50tcc gtt gac acc ggc gtt tgt gca aca tca gtc cgc aag ttc aac gag 307Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val Arg Lys Phe Asn Glu
55 60 65gca gca gca agc ctg gaa aac acc acc gtg ctg tgc atc tcc aag gat 355Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu Cys Ile Ser Lys Asp70 75 80 85ctt cca ttc gca ctg ggc cgt ttc tgc tcc gca gaa ggc atc gag aac 403Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala Glu Gly Ile Glu Asn
90 95 100gtc acc cca gta tcc gca ttc cgt tcc acc ttc ggt gaa gac aac ggc 451Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe Gly Glu Asp Asn Gly
105 110 115atc gtg ctc gaa ggc tca cca ctt aag ggt ctt ctt gca cgc agc gtc 499Ile Val Leu Glu Gly Ser Pro Leu Lys Gly Leu Leu Ala Arg Ser Val
120 125 130atc gtc gtc gat gaa aac ggc aag gtt gct tac acc cag ttg gtt gat 547Ile Val Val Asp Glu Asn Gly Lys Val Ala Tyr Thr Gln Leu Val Asp
135 140 145gag atc ttc act gaa cct gat tac gac gct gca ctt gct ggg ctg aac 595Glu Ile Phe Thr Glu Pro Asp Tyr Asp Ala Ala Leu Ala Gly Leu Asn150 155 160 165taatttactt cgctcagggg aat 618<210>172<211>165<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>172Met Ala Lys Thr His Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu1 5 10 15Leu Pro Gln Val Gly Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr
20 25 30Glu Leu Gly Glu Val Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val
35 40 45Leu Asn Ile Phe Pro Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val
50 55 60Arg Lys Phe Asn Glu Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu65 70 75 80Cys Ile Ser Lys Asp Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala
85 90 95Glu Gly Ile Glu Asn Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe
100 105 110Gly Glu Asp Asn Gly Ile Val Leu Glu Gly Ser Pro Leu Lys Gly Leu
115 120 125Leu Ala Arg Ser Val Ile Val Val Asp Glu Asn Gly Lys Val Ala Tyr
130 135 140Thr Gln Leu Val Asp Glu Ile Phe Thr Glu Pro Asp Tyr Asp Ala Ala145 150 155 160Leu Ala Gly Leu Asn
165<210>173<211>469<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(469)<223>FRXA00833<400>173agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct 60tttctagcac caaaacaaaa ctctccctag tatggggtcc atg gct aaa aca cat 115
Met Ala Lys Thr His
1 5ttt caa ggc aac gaa act gct acc tcc ggc gaa ctg cca cag gtc ggc 163Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu Leu Pro Gln Val Gly
10 15 20gac aac ctc gca gag ttc aac ctc gtc aac acc gaa ctg ggc gag gtc 211Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr Glu Leu Gly Glu Val
25 30 35tcc tca aag gac ttc cag ggc cgc aag ctt gtc ctg aac atc ttc cca 259Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val Leu Asn Ile Phe Pro
40 45 50tcc gtt gac acc ggc gtt tgt gca aca tca gtc cgc aag ttc aac gag 307Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val Arg Lys Phe Asn Glu
55 60 65gca gca gca agc ctg gaa aac acc acc gtg ctg tgc atc tcc aag gat 355Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu Cys Ile Ser Lys Asp70 75 80 85ctt cca ttc gca ctg ggc cgt ttc tgc tcc gca gaa ggc atc gag aac 403Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala Glu Gly Ile Glu Asn
90 95 100gtc acc cca gta tcc gca ttc cgt tcc acc ttc ggt gaa gac aac ggc 451Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe Gly Glu Asp Asn Gly
105 110 115atc gtg ctc gaa ggc tca 469Ile Val Leu Glu Gly Ser
120<210>174<211>123<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>174Met Ala Lys Thr His Phe Gln Gly Asn Glu Thr Ala Thr Ser Gly Glu1 5 10 15Leu Pro Gln Val Gly Asp Asn Leu Ala Glu Phe Asn Leu Val Asn Thr
20 25 30Glu Leu Gly Glu Val Ser Ser Lys Asp Phe Gln Gly Arg Lys Leu Val
35 40 45Leu Asn Ile Phe Pro Ser Val Asp Thr Gly Val Cys Ala Thr Ser Val
50 55 60Arg Lys Phe Asn Glu Ala Ala Ala Ser Leu Glu Asn Thr Thr Val Leu65 70 75 80Cys Ile Ser Lys Asp Leu Pro Phe Ala Leu Gly Arg Phe Cys Ser Ala
85 90 95Glu Gly Ile Glu Asn Val Thr Pro Val Ser Ala Phe Arg Ser Thr Phe
100 105 110Gly Glu Asp Asn Gly Ile Val Leu Glu Gly Ser
115 120<210>175<211>1146<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1123)<223>RXA01224<400>175ttggcggcgg gaagttcagg cttgggggca aacagtgctt ggattttaga caaaaaactc 60acggaagtca tcctatggca ggcgcgccta ggatggtgcc atg agc atc ctt gac 115
Met Ser Ile Leu Asp
1 5acg ttg aaa act ccc gtg att gtc gcc ccg atg gct ggc ggc ccg tcc 163Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met Ala Gly Gly Pro Ser
10 15 20act ccc gcg ttg gtc aat gca gca gca gag gca ggt tcc ctc ggg ttc 211Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala Gly Ser Leu Gly Phe
25 30 35ttg gct ggt ggc gtc atg cct ctt gag cag ctg aaa cag gaa ttg tca 259Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu Lys Gln Glu Leu Ser
40 45 50gag gta aaa ggc gtc ttt ggc gtc aac ctg ttt cgc ccg cag acg gat 307Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe Arg Pro Gln Thr Asp
55 60 65gcg cct aag cct tca gac att gat gag ctg gcg gga ttg ttg tcc tcg 355Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala Gly Leu Leu Ser Ser70 75 80 85gcg ttt cgg caa ttt ggc ctc gat gag ccg acg gtg cct acg ccg gat 403Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr Val Pro Thr Pro Asp
90 95 100ttg agc aat ggg tgg gag gct aaa ttt gag gcc gtt ctt gcc gct aag 451Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala Val Leu Ala Ala Lys
105 110 115ccc gcc gtt ttt tcc tgc acc ttt ggt att ttt agc gct gaa gaa ttc 499Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe Ser Ala Glu Glu Phe
120 125 130gcc cgg atc aaa gcc acc gga att gag gcg tgg gtg acg gtg acc aat 547Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp Val Thr Val Thr Asn
135 140 145ccg gag gac gcg ctg gct gcg cag aaa gct ggc gcc aac gcg ctt gtc 595Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly Ala Asn Ala Leu Val150 155 160 165gtg caa ggc ccc gag gcg ggt ggg cac cgc tct acc tgg tcc att gaa 643Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser Thr Trp Ser Ile Glu
170 175 180gtg gag ccg gac gag cgc gac ctg aaa acc ctc ctc gca gct gtc aaa 691Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu Leu Ala Ala Val Lys
185 190 195caa gcg ggc gtt tac ctc ccg ctc atc gca gcc ggc ggc ctt tca acc 739Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala Gly Gly Leu Ser Thr
200 205 210tcc gca gac gtg gca gca att tta gaa gcc ggc gcc agc gct gcc tcc 787Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly Ala Ser Ala Ala Ser
215 220 225tgt ggt tcc gcc ttt ttg ctt agc gac gaa gcc ggc acc agc tca ctt 835Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala Gly Thr Ser Ser Leu230 235 240 245aac cgc gag atc ttg gac gcc gcc cca gca ctt ggt ttg gaa tcg gtg 883Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu Gly Leu Glu Ser Val
250 255 260tca tct cgc gca ttt tcg ggc cgt tat gcc agg gga gtg gaa acc agg 931Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg Gly Val Glu Thr Arg
265 270 275ttc acc cgt tcg aac gag ggg tta ccc ccg ttg tac cca tac ctc aac 979Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu Tyr Pro Tyr Leu Asn
280 285 290cca atg atc aca tct tta cgt aag gtg gcg gga agt gca ggg aac tgg 1027Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly Ser Ala Gly Asn Trp
295 300 305gat tac gcc tac tgc ctg gta gga gtc ggc ctg gaa tcg att gcg aag 1075Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu Glu Ser Ile Ala Lys310 315 320 325ggt agt gca aag cag ata ctg gaa tca tta aca cct tcc gct ttg ggc 1123Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr Pro Ser Ala Leu Gly
330 335 340taatgttggg gggagtgctt tca 1146<210>176<211>341<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>176Met Ser Ile Leu Asp Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met1 5 10 15Ala Gly Gly Pro Ser Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala
20 25 30Gly Ser Leu Gly Phe Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu
35 40 45Lys Gln Glu Leu Ser Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe
50 55 60Arg Pro Gln Thr Asp Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala65 70 75 80Gly Leu Leu Ser Ser Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr
85 90 95Val Pro Thr Pro Asp Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala
100 105 110Val Leu Ala Ala Lys Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe
115 120 125Ser Ala Glu Glu Phe Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp
130 135 140Val Thr Val Thr Asn Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly145 150 155 160Ala Asn Ala Leu Val Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser
165 170 175Thr Trp Ser Ile Glu Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu
180 185 190Leu Ala Ala Val Lys Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala
195 200 205Gly Gly Leu Ser Thr Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly
210 215 220Ala Ser Ala Ala Ser Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala225 230 235 240Gly Thr Ser Ser Leu Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu
245 250 255Gly Leu Glu Ser Val Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg
260 265 270Gly Val Glu Thr Arg Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu
275 280 285Tyr Pro Tyr Leu Asn Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly
290 295 300Ser Ala Gly Asn Trp Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu305 310 315 320Glu Ser Ile Ala Lys Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr
325 330 335Pro Ser Ala Leu Gly
340<210>177<211>516<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(493)<223>RXA01182<400>177gttaaaacgg aaactaatac cccaaaggat accgattcaa tttgtgatgt gtggtgttcg 60ggtcatatca agctaaacag atgcccccta caataggctt gtg ttc aat tta ttt 115
Val Phe Asn Leu Phe
1 5ggt cgt aaa act cct cgc tct aac ctc cgc cca cca cgc ggt ccg ggc 163Gly Arg Lys Thr Pro Arg Ser Asn Leu Arg Pro Pro Arg Gly Pro Gly
10 15 20gat act gtg cgc ccg gaa gat tta aaa ttc ttg atg caa tgg gtg cag 211Asp Thr Val Arg Pro Glu Asp Leu Lys Phe Leu Met Gln Trp Val Gln
25 30 35gat aag cca ttt gtt gag gca ttc gtt gaa ccg gaa acg ctg gtc aat 259Asp Lys Pro Phe Val Glu Ala Phe Val Glu Pro Glu Thr Leu Val Asn
40 45 50gag atg tct gtc gtt ttg gtt gat gct cat ggg gtt ttt gtc cgc cga 307Glu Met Ser Val Val Leu Val Asp Ala His Gly Val Phe Val Arg Arg
55 60 65agg atc ggc ggt ccc aaa ggg att gat gtt atc gcg aaa aag ctc ggc 355Arg Ile Gly Gly Pro Lys Gly Ile Asp Val Ile Ala Lys Lys Leu Gly70 75 80 85gtt ccg gtt tat gat gtt gag gag acc ggt tac ccc caa agg atg cgc 403Val Pro Val Tyr Asp Val Glu Glu Thr Gly Tyr Pro Gln Arg Met Arg
90 95 100gaa cgc att gaa tat gag cgc atc tta aga aag cgt gag gaa caa aaa 451Glu Arg Ile Glu Tyr Glu Arg Ile Leu Arg Lys Arg Glu Glu Gln Lys
105 110 115gct cgc cgc gct aaa ttt gag cgc ggc gag aat cct gat ctt 493Ala Arg Arg Ala Lys Phe Glu Arg Gly Glu Asn Pro Asp Leu
120 125 130taactagcgt ttagctttcc gac 516<210>178<211>131<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>178Val Phe Asn Leu Phe Gly Arg Lys Thr Pro Arg Ser Asn Leu Arg Pro1 5 10 15Pro Arg Gly Pro Gly Asp Thr Val Arg Pro Glu Asp Leu Lys Phe Leu
20 25 30Met Gln Trp Val Gln Asp Lys Pro Phe Val Glu Ala Phe Val Glu Pro
35 40 45Glu Thr Leu Val Asn Glu Met Ser Val Val Leu Val Asp Ala His Gly
50 55 60Val Phe Val Arg Arg Arg Ile Gly Gly Pro Lys Gly Ile Asp Val Ile65 70 75 80Ala Lys Lys Leu Gly Val Pro Val Tyr Asp Val Glu Glu Thr Gly Tyr
85 90 95Pro Gln Arg Met Arg Glu Arg Ile Glu Tyr Glu Arg Ile Leu Arg Lys
100 105 110Arg Glu Glu Gln Lys Ala Arg Arg Ala Lys Phe Glu Arg Gly Glu Asn
115 120 125Pro Asp Leu
130<210>179<211>834<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(811)<223>RXA02531<400>179cacttcgctc cccaaggtac atccccgatg ccacttcttg gagccatcat cggtgccacc 60aaacacattg aagtgggcac tggagtagtg gatatgcgtt atg aaa atc cct ttg 115
Met Lys Ile Pro Leu
1 5tat atg gcc gag gaa gca gct gct ctc aat ctg ctt gcc gac ggc cga 163Tyr Met Ala Glu Glu Ala Ala Ala Leu Asn Leu Leu Ala Asp Gly Arg
10 15 20cta gcc ctc gga gtt tcc agg gga tca ccc gaa cca gcc gag aag ggt 211Leu Ala Leu Gly Val Ser Arg Gly Ser Pro Glu Pro Ala Glu Lys Gly
25 30 35tgg gaa gct ttc ggc tac gac ggc ggt gat gat cct aaa gct gca ggc 259Trp Glu Ala Phe Gly Tyr Asp Gly Gly Asp Asp Pro Lys Ala Ala Gly
40 45 50atg gca cgg gag aaa ttc ctt cgc ttc ctc gat gcc atc gat ggt cgc 307Met Ala Arg Glu Lys Phe Leu Arg Phe Leu Asp Ala Ile Asp Gly Arg
55 60 65ccc atg tcc atc gct tcc gag aat caa tac cca cgc ctc tac cat ccg 355Pro Met Ser Ile Ala Ser Glu Asn Gln Tyr Pro Arg Leu Tyr His Pro70 75 80 85ggc act ccc ctg ccg atc ttc ccg cat gat ctt gac ttg ggt aaa tcc 403Gly Thr Pro Leu Pro Ile Phe Pro His Asp Leu Asp Leu Gly Lys Ser
90 95 100att tgg tgg ggc gcc ggt tcc cac aac acc gcc gaa caa gca gca cgc 451Ile Trp Trp Gly Ala Gly Ser His Asn Thr Ala Glu Gln Ala Ala Arg
105 110 115gat ggc gtt aac ttg atg agc tcc acc ctc gtc gcc gaa gcc acc ggc 499Asp Gly Val Asn Leu Met Ser Ser Thr Leu Val Ala Glu Ala Thr Gly
120 125 130caa tcc ttc ggg gat ctg caa gcc gat caa atc gcg ttc tac cgc caa 547Gln Ser Phe Gly Asp Leu Gln Ala Asp Gln Ile Ala Phe Tyr Arg Gln
135 140 145gct tgg aaa gaa gcc gga cac gat tgg acc cca cgt gtg tct gtc tcc 595Ala Trp Lys Glu Ala Gly His Asp Trp Thr Pro Arg Val Ser Val Ser150 155 160 165agg tcc atc ttt ccg atc gtc acc gac cgc gac cgt gag ctt ttc gga 643Arg Ser Ile Phe Pro Ile Val Thr Asp Arg Asp Arg Glu Leu Phe Gly
170 175 180ctt cag gga caa ggc ggt gac caa gta gga atc ctg gat gat acc cga 691Leu Gln Gly Gln Gly Gly Asp Gln Val Gly Ile Leu Asp Asp Thr Arg
185 190 195tcc acg ttc ggt cgc agc tac gcc gga agt ccc gat gaa ctc atc gac 739Ser Thr Phe Gly Arg Ser Tyr Ala Gly Ser Pro Asp Glu Leu Ile Asp
200 205 210cag ctc caa gga aga caa agc tgt gat gga agc cga cac ctt gat gct 787Gln Leu Gln Gly Arg Gln Ser Cys Asp Gly Ser Arg His Leu Asp Ala
215 220 225cac cgc ccc caa cca aat ggg tgt tgagatcaac gcgtcgatcc tga 834His Arg Pro Gln Pro Asn Gly Cys230 235<210>180<211>237<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>180Met Lys Ile Pro Leu Tyr Met Ala Glu Glu Ala Ala Ala Leu Asn Leu1 5 10 15Leu Ala Asp Gly Arg Leu Ala Leu Gly Val Ser Arg Gly Ser Pro Glu
20 25 30Pro Ala Glu Lys Gly Trp Glu Ala Phe Gly Tyr Asp Gly Gly Asp Asp
35 40 45Pro Lys Ala Ala Gly Met Ala Arg Glu Lys Phe Leu Arg Phe Leu Asp
50 55 60Ala Ile Asp Gly Arg Pro Met Ser Ile Ala Ser Glu Asn Gln Tyr Pro65 70 75 80Arg Leu Tyr His Pro Gly Thr Pro Leu Pro Ile Phe Pro His Asp Leu
85 90 95Asp Leu Gly Lys Ser Ile Trp Trp Gly Ala Gly Ser His Asn Thr Ala
100 105 110Glu Gln Ala Ala Arg Asp Gly Val Asn Leu Met Ser Ser Thr Leu Val
115 120 125Ala Glu Ala Thr Gly Gln Ser Phe Gly Asp Leu Gln Ala Asp Gln Ile
130 135 140Ala Phe Tyr Arg Gln Ala Trp Lys Glu Ala Gly His Asp Trp Thr Pro145 150 155 160Arg Val Ser Val Ser Arg Ser Ile Phe Pro Ile Val Thr Asp Arg Asp
165 170 175Arg Glu Leu Phe Gly Leu Gln Gly Gln Gly Gly Asp Gln Val Gly Ile
180 185 190Leu Asp Asp Thr Arg Ser Thr Phe Gly Arg Ser Tyr Ala Gly Ser Pro
195 200 205Asp Glu Leu Ile Asp Gln Leu Gln Gly Arg Gln Ser Cys Asp Gly Ser
210 215 220Arg His Leu Asp Ala His Arg Pro Gln Pro Asn Gly Cys225 230 235<210>181<211>1614<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1591)<223>RXN00689<400>181acagggaaat cctcccagaa ttaatcaccg aagctgcaca ccagatggct actgcagacc 60tcaatcgtgc aaaggccctg ttaagaacgg atgcgatccg atg aat gct gca acc 115
Met Asn Ala Ala Thr
1 5agg cgt gct tct ctg caa ctc ccc tat acc cat gtc gat gat ttc tac 163Arg Arg Ala Ser Leu Gln Leu Pro Tyr Thr His Val Asp Asp Phe Tyr
10 15 20atc aac ggt tcc tgg gtt aaa gca gaa gga aca caa cgc aac ccc gta 211Ile Asn Gly Ser Trp Val Lys Ala Glu Gly Thr Gln Arg Asn Pro Val
25 30 35gtt gat cct gcg gtc ggt caa gaa tgg gga tct gtt cca gaa gca acc 259Val Asp Pro Ala Val Gly Gln Glu Trp Gly Ser Val Pro Glu Ala Thr
40 45 50gca tct gaa ttg gac tct gcg gtg gga gct gca cgt aca gcg cta aag 307Ala Ser Glu Leu Asp Ser Ala Val Gly Ala Ala Arg Thr Ala Leu Lys
55 60 65tcg tgg agt gca ctt aca ggt gcg gaa cga aca ggc tac ctc ctg aaa 355Ser Trp Ser Ala Leu Thr Gly Ala Glu Arg Thr Gly Tyr Leu Leu Lys70 75 80 85atc gcg acg gaa att gaa tcc cgt tct gaa gct cta gca ctt act aat 403Ile Ala Thr Glu Ile Glu Ser Arg Ser Glu Ala Leu Ala Leu Thr Asn
90 95 100acc cgc gaa aat ggt tcc ccc att tcc gag acc cgt gga gct gcg tcc 451Thr Arg Glu Asn Gly Ser Pro Ile Ser Glu Thr Arg Gly Ala Ala Ser
105 110 115aat gca gca gga att ttc cgt tac ttt gcc act ctc gcg cct tgg tta 499Asn Ala Ala Gly Ile Phe Arg Tyr Phe Ala Thr Leu Ala Pro Trp Leu
120 125 130gac ggc gaa gac atc cgc cca ttt cct gcc ggt agc gcc gaa tcc atc 547Asp Gly Glu Asp Ile Arg Pro Phe Pro Ala Gly Ser Ala Glu Ser Ile
135 140 145gtg gat aaa gat ccc atc ggt gtc tgc gca ctc atc gcc cca tgg aat 595Val Asp Lys Asp Pro Ile Gly Val Cys Ala Leu Ile Ala Pro Trp Asn150 155 160 165ttc ccg atc aac ctt gta gtc atc aaa ctg gca cca gca ctt ctt gcc 643Phe Pro Ile Asn Leu Val Val Ile Lys Leu Ala Pro Ala Leu Leu Ala
170 175 180ggc tgt acc gtc atc atc aaa cca gcc tcc ccc acc cca ctg tcg atc 691Gly Cys Thr Val Ile Ile Lys Pro Ala Ser Pro Thr Pro Leu Ser Ile
185 190 195cgt ttc atc atc gaa gcc atc gaa gcc gcc gga gtg cca gca ggc gta 739Arg Phe Ile Ile Glu Ala Ile Glu Ala Ala Gly Val Pro Ala Gly Val
200 205 210gtc aac cta ctc acc ggt tca ggg cgt ttc ggt gat gcc ctt gtc cgc 787Val Asn Leu Leu Thr Gly Ser Gly Arg Phe Gly Asp Ala Leu Val Arg
215 220 225cac ccc gga gta gac aag gta gcg ttt acc gga tca acg cct gtt gga 835His Pro Gly Val Asp Lys Val Ala Phe Thr Gly Ser Thr Pro Val Gly230 235 240 245aag aag atc gct gcc gcc tgc gga gaa cta ctc cga cca gtg act tta 883Lys Lys Ile Ala Ala Ala Cys Gly Glu Leu Leu Arg Pro Val Thr Leu
250 255 260gag cta ggc gga aaa tct tcc gcg att atc ctt cct gat gca gac atg 931Glu Leu Gly Gly Lys Ser Ser Ala Ile Ile Leu Pro Asp Ala Asp Met
265 270 275tca gta ctc tcg acg cgg ttg att cga tcc tgt atg cgc aac act gga 979Ser Val Leu Ser Thr Arg Leu Ile Arg Ser Cys Met Arg Asn Thr Gly
280 285 290caa acc tgc tac atc agt acc cgg att att gcc cct agc tca cgc tat 1027Gln Thr Cys Tyr Ile Ser Thr Arg Ile Ile Ala Pro Ser Ser Arg Tyr
295 300 305gcg gaa gtc gta caa aca gtg gca agc act atc gct gca ggt aga caa 1075Ala Glu Val Val Gln Thr Val Ala Ser Thr Ile Ala Ala Gly Arg Gln310 315 320 325ggt gac ccc tat gat gaa gaa acg gtt ttt ggg cca gtt gcc agc gcc 1123Gly Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly Pro Val Ala Ser Ala
330 335 340tct cag tac tca acc gtc atg tct tac att gac tcc gca cga gag gaa 1171Ser Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp Ser Ala Arg Glu Glu
345 350 355ggt gca cga gtg gtt gca ggt gga acc cgg tca atc agc ctt tct gaa 1219Gly Ala Arg Val Val Ala Gly Gly Thr Arg Ser Ile Ser Leu Ser Glu
360 365 370ggt tta gaa tca ggc gag ttt atc caa cca acc gtg ttt gcc gat gtc 1267Gly Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr Val Phe Ala Asp Val
375 380 385acc ccc gac atg cgg ata tca cgc gaa gaa atc ttc ggc cct gtt att 1315Thr Pro Asp Met Arg Ile Ser Arg Glu Glu Ile Phe Gly Pro Val Ile390 395 400 405tcc atc cta aag tac gac gat aca aac ggt gtt tcc gaa gca atc gca 1363Ser Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val Ser Glu Ala Ile Ala
410 415 420cta gcc aac aac acg aaa ttc ggt ctc ggt ggc ttg gta ttt ggt gcg 1411Leu Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly Leu Val Phe Gly Ala
425 430 435gat gag gaa caa gca cta gaa gtc gcc cgt caa gtg gat tct ggt tcc 1459Asp Glu Glu Gln Ala Leu Glu Val Ala Arg Gln Val Asp Ser Gly Ser
440 445 450gta ggc atc aac ttc ttc ggt tcc aac cat tcc gcc cca ttt gga gga 1507Val Gly Ile Asn Phe Phe Gly Ser Asn His Ser Ala Pro Phe Gly Gly
455 460 465cgc cac gaa tcc ggt atg gga gtg gaa tac ggc atc gaa ggc ctc agt 1555Arg His Glu Ser Gly Met Gly Val Glu Tyr Gly Ile Glu Gly Leu Ser470 475 480 485gct tac ctg aca tac aag agt att cac cga acc att tagttactga 1601Ala Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr Ile
490 495aagttctcag cta 1614<210>182<211>497<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>182Met Asn Ala Ala Thr Arg Arg Ala Ser Leu Gln Leu Pro Tyr Thr His1 5 10 15Val Asp Asp Phe Tyr Ile Asn Gly Ser Trp Val Lys Ala Glu Gly Thr
20 25 30Gln Arg Asn Pro Val Val Asp Pro Ala Val Gly Gln Glu Trp Gly Ser
35 40 45Val Pro Glu Ala Thr Ala Ser Glu Leu Asp Ser Ala Val Gly Ala Ala
50 55 60Arg Thr Ala Leu Lys Ser Trp Ser Ala Leu Thr Gly Ala Glu Arg Thr65 70 75 80Gly Tyr Leu Leu Lys Ile Ala Thr Glu Ile Glu Ser Arg Ser Glu Ala
85 90 95Leu Ala Leu Thr Asn Thr Arg Glu Asn Gly Ser Pro Ile Ser Glu Thr
100 105 110Arg Gly Ala Ala Ser Asn Ala Ala Gly Ile Phe Arg Tyr Phe Ala Thr
115 120 125Leu Ala Pro Trp Leu Asp Gly Glu Asp Ile Arg Pro Phe Pro Ala Gly
130 135 140Ser Ala Glu Ser Ile Val Asp Lys Asp Pro Ile Gly Val Cys Ala Leu145 150 155 160Ile Ala Pro Trp Asn Phe Pro Ile Asn Leu Val Val Ile Lys Leu Ala
165 170 175Pro Ala Leu Leu Ala Gly Cys Thr Val Ile Ile Lys Pro Ala Ser Pro
180 185 190Thr Pro Leu Ser Ile Arg Phe Ile Ile Glu Ala Ile Glu Ala Ala Gly
195 200 205Val Pro Ala Gly Val Val Asn Leu Leu Thr Gly Ser Gly Arg Phe Gly
210 215 220Asp Ala Leu Val Arg His Pro Gly Val Asp Lys Val Ala Phe Thr Gly225 230 235 240Ser Thr Pro Val Gly Lys Lys Ile Ala Ala Ala Cys Gly Glu Leu Leu
245 250 255Arg Pro Val Thr Leu Glu Leu Gly Gly Lys Ser Ser Ala Ile Ile Leu
260 265 270Pro Asp Ala Asp Met Ser Val Leu Ser Thr Arg Leu Ile Arg Ser Cys
275 280 285Met Arg Asn Thr Gly Gln Thr Cys Tyr Ile Ser Thr Arg Ile Ile Ala
290 295 300Pro Ser Ser Arg Tyr Ala Glu Val Val Gln Thr Val Ala Ser Thr Ile305 310 315 320Ala Ala Gly Arg Gln Gly Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly
325 330 335Pro Val Ala Ser Ala Ser Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp
340 345 350Ser Ala Arg Glu Glu Gly Ala Arg Val Val Ala Gly Gly Thr Arg Ser
355 360 365Ile Ser Leu Ser Glu Gly Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr
370 375 380Val Phe Ala Asp Val Thr Pro Asp Met Arg Ile Ser Arg Glu Glu Ile385 390 395 400Phe Gly Pro Val Ile Ser Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val
405 410 415Ser Glu Ala Ile Ala Leu Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly
420 425 430Leu Val Phe Gly Ala Asp Glu Glu Gln Ala Leu Glu Val Ala Arg Gln
435 440 445Val Asp Ser Gly Ser Val Gly Ile Asn Phe Phe Gly Ser Asn His Ser
450 455 460Ala Pro Phe Gly Gly Arg His Glu Ser Gly Met Gly Val Glu Tyr Gly465 470 475 480Ile Glu Gly Leu Ser Ala Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr
485 490 495Ile<210>183<211>750<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(727)<223>FRXA00689<400>183actccgacca gtgactttag agctaggcgg aaaatcttcc gcgattatcc ttcctgatgc 60agacatgtca gtactctcga cgcggttgat tcgatcctgt atg cgc aac act gga 115
Met Arg Asn Thr Gly
1 5caa acc tgc tac atc agt acc cgg att att gcc cct agc tca cgc tat 163Gln Thr Cys Tyr Ile Ser Thr Arg Ile Ile Ala Pro Ser Ser Arg Tyr
10 15 20gcg gaa gtc gta caa aca gtg gca agc act atc gct gca ggt aga caa 211Ala Glu Val Val Gln Thr Val Ala Ser Thr Ile Ala Ala Gly Arg Gln
25 30 35ggt gac ccc tat gat gaa gaa acg gtt ttt ggg cca gtt gcc agc gcc 259Gly Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly Pro Val Ala Ser Ala
40 45 50tct cag tac tca acc gtc atg tct tac att gac tcc gca cga gag gaa 307Ser Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp Ser Ala Arg Glu Glu
55 60 65ggt gca cga gtg gtt gca ggt gga acc cgg tca atc agc ctt tct gaa 355Gly Ala Arg Val Val Ala Gly Gly Thr Arg Ser Ile Ser Leu Ser Glu70 75 80 85ggt tta gaa tca ggc gag ttt atc caa cca acc gtg ttt gcc gat gtc 403Gly Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr Val Phe Ala Asp Val
90 95 100acc ccc gac atg cgg ata tca cgc gaa gaa atc ttc ggc cct gtt att 451Thr Pro Asp Met Arg Ile Ser Arg Glu Glu Ile Phe Gly Pro Val Ile
105 110 115tcc atc cta aag tac gac gat aca aac ggt gtt tcc gaa gca atc gca 499Ser Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val Ser Glu Ala Ile Ala
120 125 130cta gcc aac aac acg aaa ttc ggt ctc ggt ggc ttg gta ttt ggt gcg 547Leu Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly Leu Val Phe Gly Ala
135 140 145gat gag gaa caa gca cta gaa gtc gcc cgt caa gtg gat tct ggt tcc 595Asp Glu Glu Gln Ala Leu Glu Val Ala Arg Gln Val Asp Ser Gly Ser150 155 160 165gta ggc atc aac ttc ttc ggt tcc aac cat tcc gcc cca ttt gga gga 643Val Gly Ile Asn Phe Phe Gly Ser Asn His Ser Ala Pro Phe Gly Gly
170 175 180cgc cac gaa tcc ggt atg gga gtg gaa tac ggc atc gaa ggc ctc agt 691Arg His Glu Ser Gly Met Gly Val Glu Tyr Gly Ile Glu Gly Leu Ser
185 190 195gct tac ctg aca tac aag agt att cac cga acc att tagttactga 737Ala Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr Ile
200 205aagttctcag cta 750<210>184<211>209<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>184Met Arg Asn Thr Gly Gln Thr Cys Tyr Ile Ser Thr Arg Ile Ile Ala1 5 10 15Pro Ser Ser Arg Tyr Ala Glu Val Val Gln Thr Val Ala Ser Thr Ile
20 25 30Ala Ala Gly Arg Gln Gly Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly
35 40 45Pro Val Ala Ser Ala Ser Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp
50 55 60Ser Ala Arg Glu Glu Gly Ala Arg Val Val Ala Gly Gly Thr Arg Ser65 70 75 80Ile Ser Leu Ser Glu Gly Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr
85 90 95Val Phe Ala Asp Val Thr Pro Asp Met Arg Ile Ser Arg Glu Glu Ile
100 105 110Phe Gly Pro Val Ile Ser Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val
115 120 125Ser Glu Ala Ile Ala Leu Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly
130 135 140Leu Val Phe Gly Ala Asp Glu Glu Gln Ala Leu Glu Val Ala Arg Gln145 150 155 160Val Asp Ser Gly Ser Val Gly Ile Asn Phe Phe Gly Ser Asn His Ser
165 170 175Ala Pro Phe Gly Gly Arg His Glu Ser Gly Met Gly Val Glu Tyr Gly
180 185 190Ile Glu Gly Leu Ser Ala Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr
195 200 205Ile<210>185<211>878<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(855)<223>RXN03128<400>185aac gga ctc gcc att ccc gac att gga ttt ggt gta ttc caa acc cca 48Asn Gly Leu Ala Ile Pro Asp Ile Gly Phe Gly Val Phe Gln Thr Pro1 5 10 15ccc gat gaa acc cga aac tcc gtt aac gct gct ctt gaa gcc ggc tat 96Pro Asp Glu Thr Arg Asn Ser Val Asn Ala Ala Leu Glu Ala Gly Tyr
20 25 30cgc cac atc gac acc gcc gcc gca tac ggc aat gaa cgt gaa gtc ggt 144Arg His Ile Asp Thr Ala Ala Ala Tyr Gly Asn Glu Arg Glu Val Gly
35 40 45gaa gca atc gca gca tcc ggc att ggc cgc gac gag atc acc atc gaa 192Glu Ala Ile Ala Ala Ser Gly Ile Gly Arg Asp Glu Ile Thr Ile Glu
50 55 60acc aaa atc tgg gtg acc gac tac ggc ttc gag gaa act ctc cac gca 240Thr Lys Ile Trp Val Thr Asp Tyr Gly Phe Glu Glu Thr Leu His Ala65 70 75 80ttc gac aag gcc aca ggc aag ctt ggt gtc gat aca ctg gac att ttg 288Phe Asp Lys Ala Thr Gly Lys Leu Gly Val Asp Thr Leu Asp Ile Leu
85 90 95atc ttg cac cag gca gtg cca agc agc ttt gat cgc acc atc gcc gcc 336Ile Leu His Gln Ala Val Pro Ser Ser Phe Asp Arg Thr Ile Ala Ala
100 105 110tac aag gcg cta gag aag ctg ctt ttc gac ggc gcg gtg cgg gca atc 384Tyr Lys Ala Leu Glu Lys Leu Leu Phe Asp Gly Ala Val Arg Ala Ile
115 120 125gga gtc agt aat ttc atg cca gag cac ctg gac aaa ctc ctt ttg gaa 432Gly Val Ser Asn Phe Met Pro Glu His Leu Asp Lys Leu Leu Leu Glu
130 135 140acc tcc att gtc cca gct ctg aac caa atc gaa tgc cac ccc tac ttc 480Thr Ser Ile Val Pro Ala Leu Asn Gln Ile Glu Cys His Pro Tyr Phe145 150 155 160cag cag cgt gac gtg ctt gcc cgc aat gag cag ctt ggc att ttg act 528Gln Gln Arg Asp Val Leu Ala Arg Asn Glu Gln Leu Gly Ile Leu Thr
165 170 175cag gcg tgg tca cca atc ggt ggc atc acc ttc tac cgc gac gga cag 576Gln Ala Trp Ser Pro Ile Gly Gly Ile Thr Phe Tyr Arg Asp Gly Gln
180 185 190ctt cca agc act cta gaa aat gag gtc atc gct gga atc gcc gca gaa 624Leu Pro Ser Thr Leu Glu Asn Glu Val Ile Ala Gly Ile Ala Ala Glu
195 200 205gtt ggc aaa aca cca gct caa gta atg ctg cgc tgg cac cta cag cgt 672Val Gly Lys Thr Pro Ala Gln Val Met Leu Arg Trp His Leu Gln Arg
210 215 220aga cgt cac gca att cca aag tct gtg acc cca tca cgc att gtg gaa 720Arg Arg His Ala Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Val Glu225 230 235 240aac ttt gag atc ttt gat ttc gaa ctc tcc gat gag caa cta cag caa 768Asn Phe Glu Ile Phe Asp Phe Glu Leu Ser Asp Glu Gln Leu Gln Gln
245 250 255atc gat gcc ctc aac acc gat ctg cgc ggt ggc cca gaa cca gag aac 816Ile Asp Ala Leu Asn Thr Asp Leu Arg Gly Gly Pro Glu Pro Glu Asn
260 265 270atc acc atg gaa aac tac tac cga gaa atc cca gaa gcc taaaggccct 865Ile Thr Met Glu Asn Tyr Tyr Arg Glu Ile Pro Glu Ala
275 280 285tagaggcgaa tgt 878<210>186<211>285<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>186Asn Gly Leu Ala Ile Pro Asp Ile Gly Phe Gly Val Phe Gln Thr Pro1 5 10 15Pro Asp Glu Thr Arg Asn Ser Val Asn Ala Ala Leu Glu Ala Gly Tyr
20 25 30Arg His Ile Asp Thr Ala Ala Ala Tyr Gly Asn Glu Arg Glu Val Gly
35 40 45Glu Ala Ile Ala Ala Ser Gly Ile Gly Arg Asp Glu Ile Thr Ile Glu
50 55 60Thr Lys Ile Trp Val Thr Asp Tyr Gly Phe Glu Glu Thr Leu His Ala65 70 75 80Phe Asp Lys Ala Thr Gly Lys Leu Gly Val Asp Thr Leu Asp Ile Leu
85 90 95Ile Leu His Gln Ala Val Pro Ser Ser Phe Asp Arg Thr Ile Ala Ala
100 105 110Tyr Lys Ala Leu Glu Lys Leu Leu Phe Asp Gly Ala Val Arg Ala Ile
115 120 125Gly Val Ser Asn Phe Met Pro Glu His Leu Asp Lys Leu Leu Leu Glu
130 135 140Thr Ser Ile Val Pro Ala Leu Asn Gln Ile Glu Cys His Pro Tyr Phe145 150 155 160Gln Gln Arg Asp Val Leu Ala Arg Asn Glu Gln Leu Gly Ile Leu Thr
165 170 175Gln Ala Trp Ser Pro Ile Gly Gly Ile Thr Phe Tyr Arg Asp Gly Gln
180 185 190Leu Pro Ser Thr Leu Glu Asn Glu Val Ile Ala Gly Ile Ala Ala Glu
195 200 205Val Gly Lys Thr Pro Ala Gln Val Met Leu Arg Trp His Leu Gln Arg
210 215 220Arg Arg His Ala Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Val Glu225 230 235 240Asn Phe Glu Ile Phe Asp Phe Glu Leu Ser Asp Glu Gln Leu Gln Gln
245 250 255Ile Asp Ala Leu Asn Thr Asp Leu Arg Gly Gly Pro Glu Pro Glu Asn
260 265 270Ile Thr Met Glu Asn Tyr Tyr Arg Glu Ile Pro Glu Ala
275 280 285<210>187<211>522<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(522)<223>FRXA02192<400>187att ccc gac att gga ttt ggt gtc ttc caa acc cca ccc gat gaa acc 48Ile Pro Asp Ile Gly Phe Gly Val Phe Gln Thr Pro Pro Asp Glu Thr1 5 10 15cga aac tcc gtt aac gct gct ctt gaa gcc ggc tat cgc cac atc gac 96Arg Asn Ser Val Asn Ala Ala Leu Glu Ala Gly Tyr Arg His Ile Asp
20 25 30acc gcg gcc gca tac ggc aat gaa cgt gaa gtc ggt gaa gca atc gca 144Thr Ala Ala Ala Tyr Gly Asn Glu Arg Glu Val Gly Glu Ala Ile Ala
35 40 45gca tcc ggc att ggc cgc gac gag atc acc atc gaa acc aaa atc tgg 192Ala Ser Gly Ile Gly Arg Asp Glu Ile Thr Ile Glu Thr Lys Ile Trp
50 55 60gtg acc gac tac ggc ttc gag gaa act ctc cac gca ttc gac aag gcc 240Val Thr Asp Tyr Gly Phe Glu Glu Thr Leu His Ala Phe Asp Lys Ala65 70 75 80aca ggc aag ctt ggt gtc gat aca ctg gac att ttg atc ttg cac cag 288Thr Gly Lys Leu Gly Val Asp Thr Leu Asp Ile Leu Ile Leu His Gln
85 90 95gca gtg cca agc agc ttt gat cgc acc atc gcc gcc tac aag gcg cta 336Ala Val Pro Ser Ser Phe Asp Arg Thr Ile Ala Ala Tyr Lys Ala Leu
100 105 110gag aag ctg ctt ttc gac ggc gcg gtg cgg gca atc gga gtc agt aat 384Glu Lys Leu Leu Phe Asp Gly Ala ValArg Ala Ile Gly Val Ser Asn
115 120 125ttc atg cca gag cac ctg gac aaa ctc ctt ttg gaa acc tcc att gtc 432Phe Met Pro Glu His Leu Asp Lys Leu Leu Leu Glu Thr Ser Ile Val
130 135 140cca gct ctg aac caa atc gaa tgc cac ccc tac ttc cag cag cgt gac 480Pro Ala Leu Asn Gln Ile Glu Cys His Pro Tyr Phe Gln Gln Arg Asp145 150 155 160gtg ctt gcc cgc aat gag cag ctt ggc att ttg act cag gcg 522Val Leu Ala Arg Asn Glu Gln Leu Gly Ile Leu Thr Gln Ala
165 170<210>188<211>174<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>188Ile Pro Asp Ile Gly Phe Gly Val Phe Gln Thr Pro Pro Asp Glu Thr1 5 10 15Arg Asn Ser Val Asn Ala Ala Leu Glu Ala Gly Tyr Arg His Ile Asp
20 25 30Thr Ala Ala Ala Tyr Gly Asn Glu Arg Glu Val Gly Glu Ala Ile Ala
35 40 45Ala Ser Gly Ile Gly Arg Asp Glu Ile Thr Ile Glu Thr Lys Ile Trp
50 55 60Val Thr Asp Tyr Gly Phe Glu Glu Thr Leu His Ala Phe Asp Lys Ala65 70 75 80Thr Gly Lys Leu Gly Val Asp Thr Leu Asp Ile Leu Ile Leu His Gln
85 90 95Ala Val Pro Ser Ser Phe Asp Arg Thr Ile Ala Ala Tyr Lys Ala Leu
100 105 110Glu Lys Leu Leu Phe Asp Gly Ala Val Arg Ala Ile Gly Val Ser Asn
115 120 125Phe Met Pro Glu His Leu Asp Lys Leu Leu Leu Glu Thr Ser Ile Val
130 135 140Pro Ala Leu Asn Gln Ile Glu Cys His Pro Tyr Phe Gln Gln Arg Asp145 150 155 160Val Leu Ala Arg Asn Glu Gln Leu Gly Ile Leu Thr Gln Ala
165 170<210>189<211>1039<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1039)<223>RXA02351<400>189tgacacttta cagactggtt ttcaactaat gacaccgaaa gaaatacacc tcaacctttt 60tgctttcggt gccgggcacc acgcggcggc gtggcgagcg gtg gag gga agc gtc 115
Val Glu Gly Ser Val
1 5gaa aag ctg ggt tta att tcc tgg tgg gag gaa ctc gcg cgc acc gct 163Glu Lys Leu Gly Leu Ile Ser Trp Trp Glu Glu Leu Ala Arg Thr Ala
10 15 20gag cgg ggc aag ctg gat gcg gtc ttt ttg gcc gat ggg cag gcg att 211Glu Arg Gly Lys Leu Asp Ala Val Phe Leu Ala Asp Gly Gln Ala Ile
25 30 35aat ccg gtc ggt ctg gag aat ggg ccg ggc tgg ttt ttg gag ccg gtg 259Asn Pro Val Gly Leu Glu Asn Gly Pro Gly Trp Phe Leu Glu Pro Val
40 45 50acc gcg ttg act gcg atg gcg cgg gcg acg aac aat att ggg ttg atc 307Thr Ala Leu Thr Ala Met Ala Arg Ala Thr Asn Asn Ile Gly Leu Ile
55 60 65agc aca att tcc agt acg ttt tgg cag ccg ttt cat gcg gcg cgg atg 355Ser Thr Ile Ser Ser Thr Phe Trp Gln Pro Phe His Ala Ala Arg Met70 75 80 85atc gcc agc ttg gat cat att tcg ggt ggg cgt gct gga atc aat gtg 403Ile Ala Ser Leu Asp His Ile Ser Gly Gly Arg Ala Gly Ile Asn Val
90 95 100gtg aca tcg atg acc gat gcg gag gcg cgt aac cac ggg atg gat gcg 451Val Thr Ser Met Thr Asp Ala Glu Ala Arg Asn His Gly Met Asp Ala
105 110 115ttg ccg ggt cac gat gtt cgc tat gcg cgc gct gcg gaa ttt att gaa 499Leu Pro Gly His Asp Val Arg Tyr Ala Arg Ala Ala Glu Phe Ile Glu
120 125 130acc atc act gcg ctg tgg gat tct tgg cct gcg gaa agt ttg gtg atg 547Thr Ile Thr Ala Leu Trp Asp Ser Trp Pro Ala Glu Ser Leu Val Met
135 140 145gat cgt gct gga aaa ttt gcg gac tcc tcg ctc att aaa tct atc gat 595Asp Arg Ala Gly Lys Phe Ala Asp Ser Ser Leu Ile Lys Ser Ile Asp150 155 160 165cat gat ggt gag ttc ttc caa gtc gct ggt ccg ctg aat atc ccc agt 643His Asp Gly Glu Phe Phe Gln Val Ala Gly Pro Leu Asn Ile Pro Ser
170 175 180cct ccg cag ggt cga ccc gta ctt ttt cag gct gga tcc tca ccg caa 691Pro Pro Gln Gly Arg Pro Val Leu Phe Gln Ala Gly Ser Ser Pro Gln
185 190 195gga cgg gaa atc gct gcg aaa tac gcc gag gca att tac tct gtg gcg 739Gly Arg Glu Ile Ala Ala Lys Tyr Ala Glu Ala Ile Tyr Ser Val Ala
200 205 210tgg gat ttg gag caa gcg caa gat tat cgc tct gat att cat gct cgt 787Trp Asp Leu Glu Gln Ala Gln Asp Tyr Arg Ser Asp Ile His Ala Arg
215 220 225gcc act gcc cag ggt cgc gag ccc atg ccg gtg ctt cct ggt ttg gtg 835Ala Thr Ala Gln Gly Arg Glu Pro Met Pro Val Leu Pro Gly Leu Val230 235 240 245act ttt gtt ggc acg acc gtg gaa gaa gcg cgt gca aaa cag cag gct 883Thr Phe Val Gly Thr Thr Val Glu Glu Ala Arg Ala Lys Gln Gln Ala
250 255 260ctt aat gcg ttg ctg ccg gtc aaa gac tca cta aat cag ttg agt ttc 931Leu Asn Ala Leu Leu Pro Val Lys Asp Ser Leu Asn Gln Leu Ser Phe
265 270 275ttt gtg ggt caa gat tgc tcg acg tgg gat ttg gat gca cct ccc cca 979Phe Val Gly Gln Asp Cys Ser Thr Trp Asp Leu Asp Ala Pro Pro Pro
280 285 290cca ctg cca ccg cta gaa gag ttt tcc ggt cct aaa ggc agg tac gaa 1027Pro Leu Pro Pro Leu Glu Glu Phe Ser Gly Pro Lys Gly Arg Tyr Glu
295 300 305acg gtc ctg cgg 1039Thr Val Leu Arg310<210>190<211>313<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>190Val Glu Gly Ser Val Glu Lys Leu Gly Leu Ile Ser Trp Trp Glu Glu1 5 10 15Leu Ala Arg Thr Ala Glu Arg Gly Lys Leu Asp Ala Val Phe Leu Ala
20 25 30Asp Gly Gln Ala Ile Asn Pro Val Gly Leu Glu Asn Gly Pro Gly Trp
35 40 45Phe Leu Glu Pro Val Thr Ala Leu Thr Ala Met Ala Arg Ala Thr Asn
50 55 60Asn Ile Gly Leu Ile Ser Thr Ile Ser Ser Thr Phe Trp Gln Pro Phe65 70 75 80His Ala Ala Arg Met Ile Ala Ser Leu Asp His Ile Ser Gly Gly Arg
85 90 95Ala Gly Ile Asn Val Val Thr Ser Met Thr Asp Ala Glu Ala Arg Asn
100 105 110His Gly Met Asp Ala Leu Pro Gly His Asp Val Arg Tyr Ala Arg Ala
115 120 125Ala Glu Phe Ile Glu Thr Ile Thr Ala Leu Trp Asp Ser Trp Pro Ala
130 135 140Glu Ser Leu Val Met Asp Arg Ala Gly Lys Phe Ala Asp Ser Ser Leu145 150 155 160Ile Lys Ser Ile Asp His Asp Gly Glu Phe Phe Gln Val Ala Gly Pro
165 170 175Leu Asn Ile Pro Ser Pro Pro Gln Gly Arg Pro Val Leu Phe Gln Ala
180 185 190Gly Ser Ser Pro Gln Gly Arg Glu Ile Ala Ala Lys Tyr Ala Glu Ala
195 200 205Ile Tyr Ser Val Ala Trp Asp Leu Glu Gln Ala Gln Asp Tyr Arg Ser
210 215 220Asp Ile His Ala Arg Ala Thr Ala Gln Gly Arg Glu Pro Met Pro Val225 230 235 240Leu Pro Gly Leu Val Thr Phe Val Gly Thr Thr Val Glu Glu Ala Arg
245 250 255Ala Lys Gln Gln Ala Leu Asn Ala Leu Leu Pro Val Lys Asp Ser Leu
260 265 270Asn Gln Leu Ser Phe Phe Val Gly Gln Asp Cys Ser Thr Trp Asp Leu
275 280 285Asp Ala Pro Pro Pro Pro Leu Pro Pro Leu Glu Glu Phe Ser Gly Pro
290 295 300Lys Gly Arg Tyr Glu Thr Val Leu Arg305 310<210>191<211>924<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(901)<223>RXN00905<400>191cgctgcccct ctatgctgct cctagttacc cctgcacaaa tagcggtttt tctcacgcat 60tctgcatcga gtcgggtcga cgtatataag gtggaaaggc atg acc caa ttc gaa 115
Met Thr Gln Phe Glu
1 5aac gcg caa gta ctt aaa gag aac atc gaa aac caa cgc gag cag atc 163Asn Ala Gln Val Leu Lys Glu Asn Ile Glu Asn Gln Arg Glu Gln Ile
10 15 20ttt acc cag ttg aaa gaa att gtg tct ttc aac tcc gtg cac agc gat 211Phe Thr Gln Leu Lys Glu Ile Val Ser Phe Asn Ser Val His Ser Asp
25 30 35cca aac cta ctg gag gac tac gcc ggc gcg aaa gaa tgg gta aaa gaa 259Pro Asn Leu Leu Glu Asp Tyr Ala Gly Ala Lys Glu Trp Val Lys Glu
40 45 50aca ctg acc aac gca ggt ctc acc gtc agc gaa ttc gct gcc gaa gat 307Thr Leu Thr Asn Ala Gly Leu Thr Val Ser Glu Phe Ala Ala Glu Asp
55 60 65gga acc acc aac ttc atc ggc acc cgc aag ggc tcc gaa ggt gca cca 355Gly Thr Thr Asn Phe Ile Gly Thr Arg Lys Gly Ser Glu Gly Ala Pro70 75 80 85aag gta ctg ctg tac agc cac ttc gac gtt gtc cca tcc ggc cct ttg 403Lys Val Leu Leu Tyr Ser His Phe Asp Val Val Pro Ser Gly Pro Leu
90 95 100gat ctc tgg gac acc aat cct ttt gaa ctc acc gag cgc gac gct ggc 451Asp Leu Trp Asp Thr Asn Pro Phe Glu Leu Thr Glu Arg Asp Ala Gly
105 110 115cac ggc acc cgc tgg tac ggc cgc ggc gcc gct gac tgc aag ggc aac 499His Gly Thr Arg Trp Tyr Gly Arg Gly Ala Ala Asp Cys Lys Gly Asn
120 125 130ctg gtc atg cac ctc gca gca ctg cgc gcc gtc gaa gcc agc ggc gac 547Leu Val Met His Leu Ala Ala Leu Arg Ala Val Glu Ala Ser Gly Asp
135 140 145acc aca ctc aac ctc acc tac gtg gtc gag ggc tcc gag gaa atg gga 595Thr Thr Leu Asn Leu Thr Tyr Val Val Glu Gly Ser Glu Glu Met Gly150 155 160 165ggc gga gcg ctc agc gcg ctc atc aag gac aag cct gag ctt ttc gac 643Gly Gly Ala Leu Ser Ala Leu Ile Lys Asp Lys Pro Glu Leu Phe Asp
170 175 180gca gat gtc atc ttg att gca gac agc gga aac gct tcc gtg ggc acc 691Ala Asp Val Ile Leu Ile Ala Asp Ser Gly Asn Ala Ser Val Gly Thr
185 190 195cca acc ttg acc act acc ctg cgc ggt ggc gga cag gtc acc gtc acc 739Pro Thr Leu Thr Thr Thr Leu Arg Gly Gly Gly Gln Val Thr Val Thr
200 205 210gtg gac acc ctt gaa ggc gct gtt cac tcc ggc cag aac ggt ggc gct 787Val Asp Thr Leu Glu Gly Ala Val His Ser Gly Gln Asn Gly Gly Ala
215 220 225gcc cca gat gct gtt gct gct ctc gtg cgc gtt ctg gat act ttg cgc 835Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val Leu Asp Thr Leu Arg230 235 240 245gat gaa cac gga cgc acc gtt atc gac ggc tgt caa cac cac cgc aaa 883Asp Glu His Gly Arg Thr Val Ile Asp Gly Cys Gln His His Arg Lys
250 255 260ctg gaa ggg cga gcc tta tgatccagag actttccgca gcg 924Leu Glu Gly Arg Ala Leu
265<210>192<211>267<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>192Met Thr Gln Phe Glu Asn Ala Gln Val Leu Lys Glu Asn Ile Glu Asn1 5 10 15Gln Arg Glu Gln Ile Phe Thr Gln Leu Lys Glu Ile Val Ser Phe Asn
20 25 30Ser Val His Ser Asp Pro Asn Leu Leu Glu Asp Tyr Ala Gly Ala Lys
35 40 45Glu Trp Val Lys Glu Thr Leu Thr Asn Ala Gly Leu Thr Val Ser Glu
50 55 60Phe Ala Ala Glu Asp Gly Thr Thr Asn Phe Ile Gly Thr Arg Lys Gly65 70 75 80Ser Glu Gly Ala Pro Lys Val Leu Leu Tyr Ser His Phe Asp Val Val
85 90 95Pro Ser Gly Pro Leu Asp Leu Trp Asp Thr Asn Pro Phe Glu Leu Thr
100 105 110Glu Arg Asp Ala Gly His Gly Thr Arg Trp Tyr Gly Arg Gly Ala Ala
115 120 125Asp Cys Lys Gly Asn Leu Val Met His Leu Ala Ala Leu Arg Ala Val
130 135 140Glu Ala Ser Gly Asp Thr Thr Leu Asn Leu Thr Tyr Val Val Glu Gly145 150 155 160Ser Glu Glu Met Gly Gly Gly Ala Leu Ser Ala Leu Ile Lys Asp Lys
165 170 175Pro Glu Leu Phe Asp Ala Asp Val Ile Leu Ile Ala Asp Ser Gly Asn
180 185 190Ala Ser Val Gly Thr Pro Thr Leu Thr Thr Thr Leu Arg Gly Gly Gly
195 200 205Gln Val Thr Val Thr Val Asp Thr Leu Glu Gly Ala Val His Ser Gly
210 215 220Gln Asn Gly Gly Ala Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val225 230 235 240Leu Asp Thr Leu Arg Asp Glu His Gly Arg Thr Val Ile Asp Gly Cys
245 250 255Gln His His Arg Lys Leu Glu Gly Arg Ala Leu
260 265<210>193<211>716<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(693)<223>FRXA00905<400>193gat cca aac cta ctg gag gac tac gcc ggc gcg aaa gaa tgg gta aaa 48Asp Pro Asn Leu Leu Glu Asp Tyr Ala Gly Ala Lys Glu Trp Val Lys1 5 10 15gaa aca ctg acc aac gca ggt ctc acc gtc agc gaa ttc gct gcc gaa 96Glu Thr Leu Thr Asn Ala Gly Leu Thr Val Ser Glu Phe Ala Ala Glu
20 25 30gat gga acc acc aac ttc atc ggc acc cgc aag ggc tcc gaa ggt gca 144Asp Gly Thr Thr Asn Phe Ile Gly Thr Arg Lys Gly Ser Glu Gly Ala
35 40 45cca aag gta ctg ctg tac agc cac ttc gac gtt gtc cca tcc ggc cct 192Pro Lys Val Leu Leu Tyr Ser His Phe Asp Val Val Pro Ser Gly Pro
50 55 60ttg gat ctc tgg gac acc aat cct ttt gaa ctc acc gag cgc gac gct 240Leu Asp Leu Trp Asp Thr Asn Pro Phe Glu Leu Thr Glu Arg Asp Ala65 70 75 80ggc cac ggc acc cgc tgg tac ggc cgc ggc gcc gct gac tgc aag ggc 288Gly His Gly Thr Arg Trp Tyr Gly Arg Gly Ala Ala Asp Cys Lys Gly
85 90 95aac ctg gtc atg cac ctc gca gca ctg cgc gcc gtc gaa gcc agc ggc 336Asn Leu Val Met His Leu Ala Ala Leu Arg Ala Val Glu Ala Ser Gly
100 105 110gac acc aca ctc aac ctc acc tac gtg gtc gag ggc tcc gag gaa atg 384Asp Thr Thr Leu Asn Leu Thr Tyr Val Val Glu Gly Ser Glu Glu Met
115 120 125gga ggc gga gcg ctc agc gcg ctc atc aag gac aag cct gag ctt ttc 432Gly Gly Gly Ala Leu Ser Ala Leu Ile Lys Asp Lys Pro Glu Leu Phe
130 135 140gac gca gat gtc atc ttg att gca gac agc gga aac gct tcc gtg ggc 480Asp Ala Asp Val Ile Leu Ile Ala Asp Ser Gly Asn Ala Ser Val Gly145 150 155 160acc cca acc ttg acc act acc ctg cgc ggt ggc gga cag gtc acc gtc 528Thr Pro Thr Leu Thr Thr Thr Leu Arg Gly Gly Gly Gln Val Thr Val
165 170 175acc gtg gac acc ctt gaa ggc gct gtt cac tcc ggc cag aac ggt ggc 576Thr Val Asp Thr Leu Glu Gly Ala Val His Ser Gly Gln Asn Gly Gly
180 185 190gct gcc cca gat gct gtt gct gct ctc gtg cgc gtt ctg gat act ttg 624Ala Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val Leu Asp Thr Leu
195 200 205cgc gat gaa cac gga cgc acc gtt atc gac ggc tgt caa cac cac cgc 672Arg Asp Glu His Gly Arg Thr Val Ile Asp Gly Cys Gln His His Arg
210 215 220aaa ctg gaa ggg cga gcc tta tgatccagag actttccgca gcg 716Lys Leu Glu Gly Arg Ala Leu225 230<210>194<211>231<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>194Asp Pro Asn Leu Leu Glu Asp Tyr Ala Gly Ala Lys Glu Trp Val Lys1 5 10 15Glu Thr Leu Thr Asn Ala Gly Leu Thr Val Ser Glu Phe Ala Ala Glu
20 25 30Asp Gly Thr Thr Asn Phe Ile Gly Thr Arg Lys Gly Ser Glu Gly Ala
35 40 45Pro Lys Val Leu Leu Tyr Ser His Phe Asp Val Val Pro Ser Gly Pro
50 55 60Leu Asp Leu Trp Asp Thr Asn Pro Phe Glu Leu Thr Glu Arg Asp Ala65 70 75 80Gly His Gly Thr Arg Trp Tyr Gly Arg Gly Ala Ala Asp Cys Lys Gly
85 90 95Asn Leu Val Met His Leu Ala Ala Leu Arg Ala Val Glu Ala Ser Gly
100 105 110Asp Thr Thr Leu Asn Leu Thr Tyr Val Val Glu Gly Ser Glu Glu Met
115 120 125Gly Gly Gly Ala Leu Ser Ala Leu Ile Lys Asp Lys Pro Glu Leu Phe
130 135 140Asp Ala Asp Val Ile Leu Ile Ala Asp Ser Gly Asn Ala Ser Val Gly145 150 155 160Thr Pro Thr Leu Thr Thr Thr Leu Arg Gly Gly Gly Gln Val Thr Val
165 170 175Thr Val Asp Thr Leu Glu Gly Ala Val His Ser Gly Gln Asn Gly Gly
180 185 190Ala Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val Leu Asp Thr Leu
195 200 205Arg Asp Glu His Gly Arg Thr Val Ile Asp Gly Cys Gln His His Arg
210 215 220Lys Leu Glu Gly Arg Ala Leu225 230<210>195<211>627<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(604)<223>RXA00906<400>195accgtggaca cccttgaagg cgctgttcac tccggccaga acggtggcgc tgccccagat 60gctgttgctg ctctcgtgcg cgttctggat actttgcgcg atg aac acg gac gca 115
Met Asn Thr Asp Ala
1 5ccg tta tcg acg gct gtc aac acc acc gca aac tgg aag ggc gag cct 163Pro Leu Ser Thr Ala Val Asn Thr Thr Ala Asn Trp Lys Gly Glu Pro
10 15 20tat gat cca gag act ttc cgc agc gat gcc ggc atc ctc gac ggt gta 211Tyr Asp Pro Glu Thr Phe Arg Ser Asp Ala Gly Ile Leu Asp Gly Val
25 30 35gac atc atg ggc gac ggc gac aac cca gca agc atg ctg tgg tcc agg 259Asp Ile Met Gly Asp Gly Asp Asn Pro Ala Ser Met Leu Trp Ser Arg
40 45 50cct gca atc tcc atc acc gga ttc act tcc acc cca gtg gca gaa gca 307Pro Ala Ile Ser Ile Thr Gly Phe Thr Ser Thr Pro Val Ala Glu Ala
55 60 65ctc aac gca gtg ccc gca acg gcg tcc gcc aag cta aac ctt cgc gtg 355Leu Asn Ala Val Pro Ala Thr Ala Ser Ala Lys Leu Asn Leu Arg Val70 75 80 85cca gca ggc ctg gaa gca aac gat gtg gcc gag aag ctg aag cag cac 403Pro Ala Gly Leu Glu Ala Asn Asp Val Ala Glu Lys Leu Lys Gln His
90 95 100ctg atc aat cac aca cct tgg ggc gca aag atc acg gtg gag atc gat 451Leu Ile Asn His Thr Pro Trp Gly Ala Lys Ile Thr Val Glu Ile Asp
105 110 115gac att aac caa ccg ttc tcc acc gat att acc ggc cct gca atg tcc 499Asp Ile Asn Gln Pro Phe Ser Thr Asp Ile Thr Gly Pro Ala Met Ser
120 125 130acc ctg gcg tcc tgc ctg agc gct gcg tac gag ggc aag gat ctt gtc 547Thr Leu Ala Ser Cys Leu Ser Ala Ala Tyr Glu Gly Lys Asp Leu Val
135 140 145acc gaa ggc agc ggc gga tcc att cca ctg tgt acc gaa ctg att gag 595Thr Glu Gly Ser Gly Gly Ser Ile Pro Leu Cys Thr Glu Leu Ile Glu150 155 160 165gtc aac cca taagcagaat tggcactcta cgg 627Val Asn Pro<210>196<211>168<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>196Met Asn Thr Asp Ala Pro Leu Ser Thr Ala Val Asn Thr Thr Ala Asn1 5 10 15Trp Lys Gly Glu Pro Tyr Asp Pro Glu Thr Phe Arg Ser Asp Ala Gly
20 25 30Ile Leu Asp Gly Val Asp Ile Met Gly Asp Gly Asp Asn Pro Ala Ser
35 40 45Met Leu Trp Ser Arg Pro Ala Ile Ser Ile Thr Gly Phe Thr Ser Thr
50 55 60Pro Val Ala Glu Ala Leu Asn Ala Val Pro Ala Thr Ala Ser Ala Lys65 70 75 80Leu Asn Leu Arg Val Pro Ala Gly Leu Glu Ala Asn Asp Val Ala Glu
85 90 95Lys Leu Lys Gln His Leu Ile Asn His Thr Pro Trp Gly Ala Lys Ile
100 105 110Thr Val Glu Ile Asp Asp Ile Asn Gln Pro Phe Ser Thr Asp Ile Thr
115 120 125Gly Pro Ala Met Ser Thr Leu Ala Ser Cys Leu Ser Ala Ala Tyr Glu
130 135 140Gly Lys Asp Leu Val Thr Glu Gly Ser Gly Gly Ser Ile Pro Leu Cys145 150 155 160Thr Glu Leu Ile Glu Val Asn Pro
165<210>197<211>246<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(223)<223>RXA00907<400>197cctgagcgct gcgtacgagg gcaaggatct tgtcaccgaa ggcagcggcg gatccattcc 60actgtgtacc gaactgattg aggtcaaccc ataagcagaa ttg gca ctc tac ggt 115
Leu Ala Leu Tyr Gly
1 5gtg gaa gaa ccc ctc acc gtt atc cac tcc gct aat gaa tct gtt gac 163Val Glu Glu Pro Leu Thr Val Ile His Ser Ala Asn Glu Ser Val Asp
10 15 20ccc aat gag att cgc gat atc gcc acc gca gaa gca ttg ttc ctg ctc 211Pro Asn Glu Ile Arg Asp Ile Ala Thr Ala Glu Ala Leu Phe Leu Leu
25 30 35aac tac acc aag tagacccaaa agcaggcgtt aac 246Asn Tyr Thr Lys
40<210>198<211>41<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>198Leu Ala Leu Tyr Gly Val Glu Glu Pro Leu Thr Val Ile His Ser Ala1 5 10 15Asn Glu Ser Val Asp Pro Asn Glu Ile Arg Asp Ile Ala Thr Ala Glu
20 25 30Ala Leu Phe Leu Leu Asn Tyr Thr Lys
35 40<210>199<211>1386<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1363)<223>RXA02101<400>199gccatggaat gctccgttga acgcaacagc cttaaataca atcccctcct ataagccaag 60agttttagtg tcgctgcgca ggtactctac tatctaatcc atg agc cgc att tca 115
Met Ser Arg Ile Ser
1 5gaa ctt cta aac aat cat ggt gtt gat ctg tcg tgg caa gag gcc gca 163Glu Leu Leu Asn Asn His Gly Val Asp Leu Ser Trp Gln Glu Ala Ala
10 15 20tat cag gat ttc cac gaa cat cct gag ctc tcc ggc ttc gaa tca gag 211Tyr Gln Asp Phe His Glu His Pro Glu Leu Ser Gly Phe Glu Ser Glu
25 30 35acc gca gat cgc att cag aaa tac ctc gag cgt ttt gat tgt gag gtg 259Thr Ala Asp Arg Ile Gln Lys Tyr Leu Glu Arg Phe Asp Cys Glu Val
40 45 50att cca aat gtt ggc ggt tac ggc att ctg gcc gtg ttc cga aat ggg 307Ile Pro Asn Val Gly Gly Tyr Gly Ile Leu Ala Val Phe Arg Asn Gly
55 60 65tcg aca gat cct ggt gcc cct gtt gcg tta atg cgc gca gat ttc gat 355Ser Thr Asp Pro Gly Ala Pro Val Ala Leu Met Arg Ala Asp Phe Asp70 75 80 85ggc ctt ccc gtc aag gaa atc acc gga gtt ccg ttt gct tcc act cgt 403Gly Leu Pro Val Lys Glu Ile Thr Gly Val Pro Phe Ala Ser Thr Arg
90 95 100atg cgt ccg cat gat ggg gca aat gtc cat gtc atg cac gca tgc ggc 451Met Arg Pro His Asp Gly Ala Asn Val His Val Met His Ala Cys Gly
105 110 115cac gat gtc cac gtc acc gcg ctg ctt ggt gcg tgt gcc att tta gat 499His Asp Val His Val Thr Ala Leu Leu Gly Ala Cys Ala Ile Leu Asp
120 125 130gag cgt cgc gat gca tgg gaa ggc acg ttc atc gcg ttg ttc cag cca 547Glu Arg Arg Asp Ala Trp Glu Gly Thr Phe Ile Ala Leu Phe Gln Pro
135 140 145tcg gag gaa aac tcc caa ggc gct aac aag atg gtc gcc ggc ggt tta 595Ser Glu Glu Asn Ser Gln Gly Ala Asn Lys Met Val Ala Gly Gly Leu150 155 160 165gtt gat ctg atc cca cgc cct gat gtg tgc ttt ggc cag cat gta gtc 643Val Asp Leu Ile Pro Arg Pro Asp Val Cys Phe Gly Gln His Val Val
170 175 180ccc ggt gct gca gga acc gtg atg agc atg cct ggc ggt gct ctc gct 691Pro Gly Ala Ala Gly Thr Val Met Ser Met Pro Gly Gly Ala Leu Ala
185 190 195gcc tgc gat tcc att gaa atc cgc att cag ggt cgc agc gcc cat ggt 739Ala Cys Asp Ser Ile Glu Ile Arg Ile Gln Gly Arg Ser Ala His Gly
200 205 210tcc atg cct cat aat tcc atc gat ccc act tat gtt gca gcg atg att 787Ser Met Pro His Asn Ser Ile Asp Pro Thr Tyr Val Ala Ala Met Ile
215 220 225gtc gtg cga ctc caa gga atc gtg ggc cgc gag gtt tct cca gag gat 835Val Val Arg Leu Gln Gly Ile Val Gly Arg Glu Val Ser Pro Glu Asp230 235 240 245ttc gcc gtt att tct gtg ggc acc ctc cag tcg ggc aac acc aac aac 883Phe Ala Val Ile Ser Val Gly Thr Leu Gln Ser Gly Asn Thr Asn Asn
250 255 260acc att cca gca agt gct cgt ttg gtg ttg aac tgc cgt ttc tac aac 931Thr Ile Pro Ala Ser Ala Arg Leu Val Leu Asn Cys Arg Phe Tyr Asn
265 270 275gac aaa gtc aag cac aag gtc tac cga gcc atc gaa cgt gtt gtc cgt 979Asp Lys Val Lys His Lys Val Tyr Arg Ala Ile Glu Arg Val Val Arg
280 285 290ggt gaa tgc ctt gct tcc ggt att gag gaa gaa cct gtc att gag tac 1027Gly Glu Cys Leu Ala Ser Gly Ile Glu Glu Glu Pro Val Ile Glu Tyr
295 300 305ttc gcc cac ggt gat ctc acc aac aac acc cct gtt gtc ttc gat act 1075Phe Ala His Gly Asp Leu Thr Asn Asn Thr Pro Val Val Phe Asp Thr310 315 320 325gtg cgc cct gtc ttc gac gat gtt ttc ggc gag gat tct att gac gct 1123Val Arg Pro Val Phe Asp Asp Val Phe Gly Glu Asp Ser Ile Asp Ala
330 335 340tac cgg tgg act gcg tcg gag gat ttc ccc tcc att cct aag gca ttc 1171Tyr Arg Trp Thr Ala Ser Glu Asp Phe Pro Ser Ile Pro Lys Ala Phe
345 350 355aac agc cct tac ctg tac tgg acg att ggt gtc acg ccg cgc gat cag 1219Asn Ser Pro Tyr Leu Tyr Trp Thr Ile Gly Val Thr Pro Arg Asp Gln
360 365 370tgg aca gaa gcc gta gaa aga gac cgc gtg gca tcg gat gtg cca gcc 1267Trp Thr Glu Ala Val Glu Arg Asp Arg Val Ala Ser Asp Val Pro Ala
375 380 385aat cac atg gga gat ttc ctc cct gat tat gcg ccg acg atg tcc gct 1315Asn His Met Gly Asp Phe Leu Pro Asp Tyr Ala Pro Thr Met Ser Ala390 395 400 405gcc acc cgc gca gcc gca gcc gcg ctg ctg acc tac ttg gga act aac 1363Ala Thr Arg Ala Ala Ala Ala Ala Leu Leu Thr Tyr Leu Gly Thr Asn
410 415 420taatcatcta gttttctgcg acg 1386<210>200<211>421<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>200Met Ser Arg Ile Ser Glu Leu Leu Asn Asn His Gly Val Asp Leu Ser1 5 10 15Trp Gln Glu Ala Ala Tyr Gln Asp Phe His Glu His Pro Glu Leu Ser
20 25 30Gly Phe Glu Ser Glu Thr Ala Asp Arg Ile Gln Lys Tyr Leu Glu Arg
35 40 45Phe Asp Cys Glu Val Ile Pro Asn Val Gly Gly Tyr Gly Ile Leu Ala
50 55 60Val Phe Arg Asn Gly Ser Thr Asp Pro Gly Ala Pro Val Ala Leu Met65 70 75 80Arg Ala Asp Phe Asp Gly Leu Pro Val Lys Glu Ile Thr Gly Val Pro
85 90 95Phe Ala Ser Thr Arg Met Arg Pro His Asp Gly Ala Asn Val His Val
100 105 110Met His Ala Cys Gly His Asp Val His Val Thr Ala Leu Leu Gly Ala
115 120 125Cys Ala Ile Leu Asp Glu Arg Arg Asp Ala Trp Glu Gly Thr Phe Ile
130 135 140Ala Leu Phe Gln Pro Ser Glu Glu Asn Ser Gln Gly Ala Asn Lys Met145 150 155 160Val Ala Gly Gly Leu Val Asp Leu Ile Pro Arg Pro Asp Val Cys Phe
165 170 175Gly Gln His Val Val Pro Gly Ala Ala Gly Thr Val Met Ser Met Pro
180 185 190Gly Gly Ala Leu Ala Ala Cys Asp Ser Ile Glu Ile Arg Ile Gln Gly
195 200 205Arg Ser Ala His Gly Ser Met Pro His Asn Ser Ile Asp Pro Thr Tyr
210 215 220Val Ala Ala Met Ile Val Val Arg Leu Gln Gly Ile Val Gly Arg Glu225 230 235 240Val Ser Pro Glu Asp Phe Ala Val Ile Ser Val Gly Thr Leu Gln Ser
245 250 255Gly Asn Thr Asn Asn Thr Ile Pro Ala Ser Ala Arg Leu Val Leu Asn
260 265 270Cys Arg Phe Tyr Asn Asp Lys Val Lys His Lys Val Tyr Arg Ala Ile
275 280 285Glu Arg Val Val Arg Gly Glu Cys Leu Ala Ser Gly Ile Glu Glu Glu
290 295 300Pro Val Ile Glu Tyr Phe Ala His Gly Asp Leu Thr Asn Asn Thr Pro305 310 315 320Val Val Phe Asp Thr Val Arg Pro Val Phe Asp Asp Val Phe Gly Glu
325 330 335Asp Ser Ile Asp Ala Tyr Arg Trp Thr Ala Ser Glu Asp Phe Pro Ser
340 345 350Ile Pro Lys Ala Phe Asn Ser Pro Tyr Leu Tyr Trp Thr Ile Gly Val
355 360 365Thr Pro Arg Asp Gln Trp Thr Glu Ala Val Glu Arg Asp Arg Val Ala
370 375 380Ser Asp Val Pro Ala Asn His Met Gly Asp Phe Leu Pro Asp Tyr Ala385 390 395 400Pro Thr Met Ser Ala Ala Thr Arg Ala Ala Ala Ala Ala Leu Leu Thr
405 410 415Tyr Leu Gly Thr Asn
420<210>201<211>1389<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1366)<223>RXN02565<400>201ggaaattcga tacagtgcga tgacgcgata ttagaaagaa aaagatgcgc tttacgacga 60aaccctcacc ctccttcagg aacttatccg caacgcctgc gtg aat gat cta acc 115
Val Asn Asp Leu Thr
1 5cca gat tca ggt cag gaa att aga aac gcg gaa agc cta gaa cgt ttc 163Pro Asp Ser Gly Gln Glu Ile Arg Asn Ala Glu Ser Leu Glu Arg Phe
10 15 20ttt gaa gga acc ccc aac gtt aaa atc acc aag ctg gaa ccg cat ccg 211Phe Glu Gly Thr Pro Asn Val Lys Ile Thr Lys Leu Glu Pro His Pro
25 30 35ggc cgg acc tca att atc gtg act gtt cca ggc agc gat cca gat gct 259Gly Arg Thr Ser Ile Ile Val Thr Val Pro Gly Ser Asp Pro Asp Ala
40 45 50gag cct tta aca ctg ctt gga cat act gat gtt gtg cct gtt gat ctg 307Glu Pro Leu Thr Leu Leu Gly His Thr Asp Val Val Pro Val Asp Leu
55 60 65cct aaa tgg act aaa gat cca ttc ggt gcg gag att tcg gat gga cag 355Pro Lys Trp Thr Lys Asp Pro Phe Gly Ala Glu Ile Ser Asp Gly Gln70 75 80 85att tgg ggt aga ggg tcc gtc gat atg ctc ttt att acc gca acc caa 403Ile Trp Gly Arg Gly Ser Val Asp Met Leu Phe Ile Thr Ala Thr Gln
90 95 100gcg gcc gtc acc cgt caa gta gcc cgt gaa ggc ggc ctg cgt ggc acg 451Ala Ala Val Thr Arg Gln Val Ala Arg Glu Gly Gly Leu Arg Gly Thr
105 110 115ctg aca ttc gtt ggc gtt gct gat gag gaa gcc cgc ggc gga ctc gga 499Leu Thr Phe Val Gly Val Ala Asp Glu Glu Ala Arg Gly Gly Leu Gly
120 125 130gcg aag tgg ctt tcc gaa gaa cac caa aac ctc ttc agc tgg aaa aac 547Ala Lys Trp Leu Ser Glu Glu His Gln Asn Leu Phe Ser Trp Lys Asn
135 140 145tgc ctc tcc gaa tcc ggt gga tcg cac ctt cca gtc cac gac ggc agc 595Cys Leu Ser Glu Ser Gly Gly Ser His Leu Pro Val His Asp Gly Ser150 155 160 165gac gca gta gta att aac gtt gga gaa aaa ggt gca gct caa cgt cgt 643Asp Ala Val Val Ile Asn Val Gly Glu Lys Gly Ala Ala Gln Arg Arg
170 175 180att cac gtc aat ggc gat gct ggt cat ggt tcc att cct ttc gac cgt 691Ile His Val Asn Gly Asp Ala Gly His Gly Ser Ile Pro Phe Asp Arg
185 190 195gac agc gct att gtc aag atc ggt gaa gtc gcc cgc cga atc gct gcc 739Asp Ser Ala Ile Val Lys Ile Gly Glu Val Ala Arg Arg Ile Ala Ala
200 205 210gcc gat ctg aag gta gcc aag gac gat atc tgg caa ggc ttc gtc caa 787Ala Asp Leu Lys Val Ala Lys Asp Asp Ile Trp Gln Gly Phe Val Gln
215 220 225gcg cac cgt ttc gac cca gaa acg gag cag gcg ctt ctt agc ggg acc 835Ala His Arg Phe Asp Pro Glu Thr Glu Gln Ala Leu Leu Ser Gly Thr230 235 240 245tcc cct gag gcc tac gca gag ttc ggc gga ctc tcc cgc ttc gcc cac 883Ser Pro Glu Ala Tyr Ala Glu Phe Gly Gly Leu Ser Arg Phe Ala His
250 255 260gcg gtg tct cat ctc acg atc gcc caa act gtg gtt cgt gca ggt caa 931Ala Val Ser His Leu Thr Ile Ala Gln Thr Val Val Arg Ala Gly Gln
265 270 275gcc atc aat gta ttg cca tcg cat gcg tac ttg gaa ctg gat atc cgt 979Ala Ile Asn Val Leu Pro Ser His Ala Tyr Leu Glu Leu Asp Ile Arg
280 285 290acc ctt cca ggc caa acc aat gac tat gtt gat gac acc ctg cgt gct 1027Thr Leu Pro Gly Gln Thr Asn Asp Tyr Val Asp Asp Thr Leu Arg Ala
295 300 305gct ctg ggc gat ctt gcc gat gaa gta gaa atc gaa cac ctc atc tct 1075Ala Leu Gly Asp Leu Ala Asp Glu Val Glu Ile Glu His Leu Ile Ser310 315 320 325gaa gaa gca acg gtg agc cca act gat tcc agg ttg tat aac acc ttg 1123Glu Glu Ala Thr Val Ser Pro Thr Asp Ser Arg Leu Tyr Asn Thr Leu
330 335 340gaa aaa gtt ctt ggt gat ttc ttc ccc gat gcg cct gtg gtc cca att 1171Glu Lys Val Leu Gly Asp Phe Phe Pro Asp Ala Pro Val Val Pro Ile
345 350 355att tcc tct ggt ggc tct gac ctg cgc ttt ggt cgt cga cta ggc ggt 1219Ile Ser Ser Gly Gly Ser Asp Leu Arg Phe Gly Arg Arg Leu Gly Gly
360 365 370gtt ggt tat ggt ttt gca gtt cat gca cgt gaa cga act ttg gcg gaa 1267Val Gly Tyr Gly Phe Ala Val His Ala Arg Glu Arg Thr Leu Ala Glu
375 380 385gca atg ggg caa ctt cac tcc cat gac gag gcg ctg tac ctg gaa gat 1315Ala Met Gly Gln Leu His Ser His Asp Glu Ala Leu Tyr Leu Glu Asp390 395 400 405ctt gaa ctg act gtt cgg ggt tat gac tcc gtc gtg cgt gaa ttc cta 1363Leu Glu Leu Thr Val Arg Gly Tyr Asp Ser Val Val Arg Glu Phe Leu
410 415 420ggc taaaaacatg aagcaggagt ctt 1389Gly<210>202<211>422<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>202Val Asn Asp Leu Thr Pro Asp Ser Gly Gln Glu Ile Arg Asn Ala Glu1 5 10 15Ser Leu Glu Arg Phe Phe Glu Gly Thr Pro Asn Val Lys Ile Thr Lys
20 25 30Leu Glu Pro His Pro Gly Arg Thr Ser Ile Ile Val Thr Val Pro Gly
35 40 45Ser Asp Pro Asp Ala Glu Pro Leu Thr Leu Leu Gly His Thr Asp Val
50 55 60Val Pro Val Asp Leu Pro Lys Trp Thr Lys Asp Pro Phe Gly Ala Glu65 70 75 80Ile Ser Asp Gly Gln Ile Trp Gly Arg Gly Ser Val Asp Met Leu Phe
85 90 95Ile Thr Ala Thr Gln Ala Ala Val Thr Arg Gln Val Ala Arg Glu Gly
100 105 110Gly Leu Arg Gly Thr Leu Thr Phe Val Gly Val Ala Asp Glu Glu Ala
115 120 125Arg Gly Gly Leu Gly Ala Lys Trp Leu Ser Glu Glu His Gln Asn Leu
130 135 140Phe Ser Trp Lys Asn Cys Leu Ser Glu Ser Gly Gly Ser His Leu Pro145 150 155 160Val His Asp Gly Ser Asp Ala Val Val Ile Asn Val Gly Glu Lys Gly
165 170 175Ala Ala Gln Arg Arg Ile His Val Asn Gly Asp Ala Gly His Gly Ser
180 185 190Ile Pro Phe Asp Arg Asp Ser Ala Ile Val Lys Ile Gly Glu Val Ala
195 200 205Arg Arg Ile Ala Ala Ala Asp Leu Lys Val Ala Lys Asp Asp Ile Trp
210 215 220Gln Gly Phe Val Gln Ala His Arg Phe Asp Pro Glu Thr Glu Gln Ala225 230 235 240Leu Leu Ser Gly Thr Ser Pro Glu Ala Tyr Ala Glu Phe Gly Gly Leu
245 250 255Ser Arg Phe Ala His Ala Val Ser His Leu Thr Ile Ala Gln Thr Val
260 265 270Val Arg Ala Gly Gln Ala Ile Asn Val Leu Pro Ser His Ala Tyr Leu
275 280 285Glu Leu Asp Ile Arg Thr Leu Pro Gly Gln Thr Asn Asp Tyr Val Asp
290 295 300Asp Thr Leu Arg Ala Ala Leu Gly Asp Leu Ala Asp Glu Val Glu Ile305 310 315 320Glu His Leu Ile Ser Glu Glu Ala Thr Val Ser Pro Thr Asp Ser Arg
325 330 335Leu Tyr Asn Thr Leu Glu Lys Val Leu Gly Asp Phe Phe Pro Asp Ala
340 345 350Pro Val Val Pro Ile Ile Ser Ser Gly Gly Ser Asp Leu Arg Phe Gly
355 360 365Arg Arg Leu Gly Gly Val Gly Tyr Gly Phe Ala Val His Ala Arg Glu
370 375 380Arg Thr Leu Ala Glu Ala Met Gly Gln Leu His Ser His Asp Glu Ala385 390 395 400Leu Tyr Leu Glu Asp Leu Glu Leu Thr Val Arg Gly Tyr Asp Ser Val
405 410 415Val Arg Glu Phe Leu Gly
420<210>203<211>365<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(342)<223>FRXA02565<400>203gct gct ctg ggc gat ctt gcc gat gaa gta gaa atc gaa cac ctc atc 48Ala Ala Leu Gly Asp Leu Ala Asp Glu Val Glu Ile Glu His Leu Ile1 5 10 15tct gaa gaa gca acg gtg agc cca act gat tcc agg ttg tat aac acc 96Ser Glu Glu Ala Thr Val Ser Pro Thr Asp Ser Arg Leu Tyr Asn Thr
20 25 30ttg gaa aaa gtt ctt ggt gat ttc ttc ccc gat gcg cct gtg gtc cca 144Leu Glu Lys Val Leu Gly Asp Phe Phe Pro Asp Ala Pro Val Val Pro
35 40 45att att tcc tct ggt ggc tct gac ctg cgc ttt ggt cgt cga cta ggc 192Ile Ile Ser Ser Gly Gly Ser Asp Leu Arg Phe Gly Arg Arg Leu Gly
50 55 60ggt gtt ggt tat ggt ttt gca gtt cat gca cgt gaa cga act ttg gcg 240Gly Val Gly Tyr Gly Phe Ala Val His Ala Arg Glu Arg Thr Leu Ala65 70 75 80gaa gca atg ggg caa ctt cac tcc cat gac gag gcg ctg tac ctg gaa 288Glu Ala Met Gly Gln Leu His Ser His Asp Glu Ala Leu Tyr Leu Glu
85 90 95gat ctt gaa ctg act gtt cgg ggt tat gac tcc gtc gtg cgt gaa ttc 336Asp Leu Glu Leu Thr Val Arg Gly Tyr Asp Ser Val Val Arg Glu Phe
100 105 110cta ggc taaaaacatg aagcaggagt ctt 365Leu Gly<210>204<211>114<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>204Ala Ala Leu Gly Asp Leu Ala Asp Glu Val Glu Ile Glu His Leu Ile1 5 10 15Ser Glu Glu Ala Thr Val Ser Pro Thr Asp Ser Arg Leu Tyr Asn Thr
20 25 30Leu Glu Lys Val Leu Gly Asp Phe Phe Pro Asp Ala Pro Val Val Pro
35 40 45Ile Ile Ser Ser Gly Gly Ser Asp Leu Arg Phe Gly Arg Arg Leu Gly
50 55 60Gly Val Gly Tyr Gly Phe Ala Val His Ala Arg Glu Arg Thr Leu Ala65 70 75 80Glu Ala Met Gly Gln Leu His Ser His Asp Glu Ala Leu Tyr Leu Glu
85 90 95Asp Leu Glu Leu Thr Val Arg Gly Tyr Asp Ser Val Val Arg Glu Phe
100 105 110Leu Gly<210>205<211>738<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(738)<223>FRXA02567<400>205ctt atc cgc aac gcc tgc gtg aat gat cta acc cca gat tca ggt cag 48Leu Ile Arg Asn Ala Cys Val Asn Asp Leu Thr Pro Asp Ser Gly Gln1 5 10 15gaa att aga aac gcg gaa agc cta gaa cgt ttc ttt gaa gga acc ccc 96Glu Ile Arg Asn Ala Glu Ser Leu Glu Arg Phe Phe Glu Gly Thr Pro
20 25 30aac gtt aaa atc acc aag ctg gaa ccg cat ccg ggc cgg acc tca att 144Asn Val Lys Ile Thr Lys Leu Glu Pro His Pro Gly Arg Thr Ser Ile
35 40 45atc gtg act gtt cca ggc agc gat cca gat gct gag cct tta aca ctg 192Ile Val Thr Val Pro Gly Ser Asp Pro Asp Ala Glu Pro Leu Thr Leu
50 55 60ctt gga cat act gat gtt gtg cct gtt gat ctg cct aaa tgg act aaa 240Leu Gly His Thr Asp Val Val Pro Val Asp Leu Pro Lys Trp Thr Lys65 70 75 80gat cca ttc ggt gcg gag att tcg gat gga cag att tgg ggt aga ggg 288Asp Pro Phe Gly Ala Glu Ile Ser Asp Gly Gln Ile Trp Gly Arg Gly
85 90 95tcc gtc gat atg ctc ttt att acc gca acc caa gcg gcc gtc acc cgt 336Ser Val Asp Met Leu Phe Ile Thr Ala Thr Gln Ala Ala Val Thr Arg
100 105 110caa gta gcc cgt gaa ggc ggc ctg cgt ggc acg ctg aca ttc gtt ggc 384Gln Val Ala Arg Glu Gly Gly Leu Arg Gly Thr Leu Thr Phe Val Gly
115 120 125gtt gct gat gag gaa gcc cgc ggc gga ctc gga gcg aag tgg ctt tcc 432Val Ala Asp Glu Glu Ala Arg Gly Gly Leu Gly Ala Lys Trp Leu Ser
130 135 140gaa gaa cac caa aac ctc ttc agc tgg aaa aac tgc ctc tcc gaa tcc 480Glu Glu His Gln Asn Leu Phe Ser Trp Lys Asn Cys Leu Ser Glu Ser145 150 155 160ggt gga tcg cac ctt cca gtc cac gac ggc agc gac gca gta gta att 528Gly Gly Ser His Leu Pro Val His Asp Gly Ser Asp Ala Val Val Ile
165 170 175aac gtt gga gaa aaa ggt gca gct caa cgt cgt att cac gtc aat ggc 576Asn Val Gly Glu Lys Gly Ala Ala Gln Arg Arg Ile His Val Asn Gly
180 185 190gat gct ggt cat ggt tcc att cct ttc gac cgt gac agc gct att gtc 624Asp Ala Gly His Gly Ser Ile Pro Phe Asp Arg Asp Ser Ala Ile Val
195 200 205aag atc ggt gaa gtc gcc cgc cga atc gct gcc gcc gat ctg aag gta 672Lys Ile Gly Glu Val Ala Arg Arg Ile Ala Ala Ala Asp Leu Lys Val
210 215 220gcc aag gac gat atc tgg caa ggc ttc gtc caa gcg cac cgt ttc gac 720Ala Lys Asp Asp Ile Trp Gln Gly Phe Val Gln Ala His Arg Phe Asp225 230 235 240cca gaa acg gag cag gcg 738Pro Glu Thr Glu Gln Ala
245<210>206<211>246<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>206Leu Ile Arg Asn Ala Cys Val Asn Asp Leu Thr Pro Asp Ser Gly Gln1 5 10 15Glu Ile Arg Asn Ala Glu Ser Leu Glu Arg Phe Phe Glu Gly Thr Pro
20 25 30Asn Val Lys Ile Thr Lys Leu Glu Pro His Pro Gly Arg Thr Ser Ile
35 40 45Ile Val Thr Val Pro Gly Ser Asp Pro Asp Ala Glu Pro Leu Thr Leu
50 55 60Leu Gly His Thr Asp Val Val Pro Val Asp Leu Pro Lys Trp Thr Lys65 70 75 80Asp Pro Phe Gly Ala Glu Ile Ser Asp Gly Gln Ile Trp Gly Arg Gly
85 90 95Ser Val Asp Met Leu Phe Ile Thr Ala Thr Gln Ala Ala Val Thr Arg
100 105 110Gln Val Ala Arg Glu Gly Gly Leu Arg Gly Thr Leu Thr Phe Val Gly
115 120 125Val Ala Asp Glu Glu Ala Arg Gly Gly Leu Gly Ala Lys Trp Leu Ser
130 135 140Glu Glu His Gln Asn Leu Phe Ser Trp Lys Asn Cys Leu Ser Glu Ser145 150 155 160Gly Gly Ser His Leu Pro Val His Asp Gly Ser Asp Ala Val Val Ile
165 170 175Asn Val Gly Glu Lys Gly Ala Ala Gln Arg Arg Ile His Val Asn Gly
180 185 190Asp Ala Gly His Gly Ser Ile Pro Phe Asp Arg Asp Ser Ala Ile Val
195 200 205Lys Ile Gly Glu Val Ala Arg Arg Ile Ala Ala Ala Asp Leu Lys Val
210 215 220Ala Lys Asp Asp Ile Trp Gln Gly Phe Val Gln Ala His Arg Phe Asp225 230 235 240Pro Glu Thr Glu Gln Ala
245<210>207<211>1308<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1285)<223>RXN03077<400>207accccgatcc tttgttttcg tgggatcact attagactcg actctaccgc gctgcaggtt 60ttcctgatac gcctgcggac aaaacagaaa ggtatttcac gtg atg gaa att ggt 115
Val Met Glu Ile Gly
1 5gtg cag gtt gcc tca tgg atg gac cgc cac cat gac gag gtc ata aag 163Val Gln Val Ala Ser Trp Met Asp Arg His His Asp Glu Val Ile Lys
10 15 20tgg cgc agg cat ttg cac agc cat cct gag ctc tcc cac atg gaa tac 211Trp Arg Arg His Leu His Ser His Pro Glu Leu Ser His Met Glu Tyr
25 30 35cgc acg act gag tat ttg gcc tcg gtt ctg aaa gat cac ggc atg gaa 259Arg Thr Thr Glu Tyr Leu Ala Ser Val Leu Lys Asp His Gly Met Glu
40 45 50cca cac ctg ttc cca gga acc ggt ttg atg gtg gat atc gga cca gaa 307Pro His Leu Phe Pro Gly Thr Gly Leu Met Val Asp Ile Gly Pro Glu
55 60 65ggg gac tcc cgc ctg gcg ttt cgc gct gat atc gat gcc ctt ccg ctg 355Gly Asp Ser Arg Leu Ala Phe Arg Ala Asp Ile Asp Ala Leu Pro Leu70 75 80 85ctt gaa tca acc ggc tta gag ttc tct tcc aca gcc act ggc gtt gcg 403Leu Glu Ser Thr Gly Leu Glu Phe Ser Ser Thr Ala Thr Gly Val Ala
90 95 100cat gcc tgc gga cat gac gtg cac acg gtg atc gct ttg gca ctt gcc 451His Ala Cys Gly His Asp Val His Thr Val Ile Ala Leu Ala Leu Ala
105 110 115tgt gca ctg aac acc atc gaa ctg ccc atc ggc att cgg gtg att ttc 499Cys Ala Leu Asn Thr Ile Glu Leu Pro Ile Gly Ile Arg Val Ile Phe
120 125 130cag ccg gca gaa gaa gtc atg act ggt ggc gca acg gac gtc att gcc 547Gln Pro Ala Glu Glu Val Met Thr Gly Gly Ala Thr Asp Val Ile Ala
135 140 145cac ggt ggc ctt gat ggt gtg gat gcg att tac gcc atc cac gtt gaa 595His Gly Gly Leu Asp Gly Val Asp Ala Ile Tyr Ala Ile His Val Glu150 155 160 165ccc aaa ttg aag gtc ggt cgc gtc ggt gta cgc gct ggc gcg att act 643Pro Lys Leu Lys Val Gly Arg Val Gly Val Arg Ala Gly Ala Ile Thr
170 175 180tct gcc tca gat gtg atc gaa atc aga gtc aag ggt gaa gga gga cat 691Ser Ala Ser Asp Val Ile Glu Ile Arg Val Lys Gly Glu Gly Gly His
185 190 195agc gca cgt cca cac ctc tcc gct gat gtt gtt tac gcc ttg agc aaa 739Ser Ala Arg Pro His Leu Ser Ala Asp Val Val Tyr Ala Leu Ser Lys
200 205 210ttg gtc gtt gat ctt ccc ggt ttg ctg tcc agg cgc gtc gat cca cgc 787Leu Val Val Asp Leu Pro Gly Leu Leu Ser Arg Arg Val Asp Pro Arg
215 220 225acc ggc acc gtg ctt gtt ttc ggc acc atc aac gcc ggc tat gcg ccc 835Thr Gly Thr Val Leu Val Phe Gly Thr Ile Asn Ala Gly Tyr Ala Pro230 235 240 245aac gcg atc cca gat tcc ggc atc gtg tca ggc acc ttg cgt aca gcc 883Asn Ala Ile Pro Asp Ser Gly Ile Val Ser Gly Thr Leu Arg Thr Ala
250 255 260gac atc tct acc tgg cgt gac atg cgt ccg ctt atc tct gag ctg gtg 931Asp Ile Ser Thr Trp Arg Asp Met Arg Pro Leu Ile Ser Glu Leu Val
265 270 275gaa cag gtg ctc gca ccc acc gga gtc acc cat gaa ctg atc tac aat 979Glu Gln Val Leu Ala Pro Thr Gly Val Thr His Glu Leu Ile Tyr Asn
280 285 290ccg ggt gtt cca cca gtg ctt aac gac gat gtc gcc acc gct ttg ttg 1027Pro Gly Val Pro Pro Val Leu Asn Asp Asp Val Ala Thr Ala Leu Leu
295 300 305gca agc gca gca cgc gac atg gac aca caa tct gtt gtc caa gcg ccg 1075Ala Ser Ala Ala Arg Asp Met Asp Thr Gln Ser Val Val Gln Ala Pro310 315 320 325cag tca tcc ggt gga gaa gac ttc tcg tgg tac ctt gaa cac gtc cca 1123Gln Ser Ser Gly Gly Glu Asp Phe Ser Trp Tyr Leu Glu His Val Pro
330 335 340gga tca atg gcc cgg ttg ggt tgc tgg ccg ggg cac gga ccc aag caa 1171Gly Ser Met Ala Arg Leu Gly Cys Trp Pro Gly His Gly Pro Lys Gln
345 350 355gac ctc cat caa agt gac ctg gtt gtg gat gag cga gcc atc gga gtt 1219Asp Leu His Gln Ser Asp Leu Val Val Asp Glu Arg Ala Ile Gly Val
360 365 370ggc gtc agg ctc ttt ggc tcc ctt gtg cag cag tac agt agc cga tct 1267Gly Val Arg Leu Phe Gly Ser Leu Val Gln Gln Tyr Ser Ser Arg Ser
375 380 385gaa gct ttc tta aat tcc taatgggggt agtgtgtagg gct 1308Glu Ala Phe Leu Asn Ser390 395<210>208<211>395<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>208Val Met Glu Ile Gly Val Gln Val Ala Ser Trp Met Asp Arg His His1 5 10 15Asp Glu Val Ile Lys Trp Arg Arg His Leu His Ser His Pro Glu Leu
20 25 30Ser His Met Glu Tyr Arg Thr Thr Glu Tyr Leu Ala Ser Val Leu Lys
35 40 45Asp His Gly Met Glu Pro His Leu Phe Pro Gly Thr Gly Leu Met Val
50 55 60Asp Ile Gly Pro Glu Gly Asp Ser Arg Leu Ala Phe Arg Ala Asp Ile65 70 75 80Asp Ala Leu Pro Leu Leu Glu Ser Thr Gly Leu Glu Phe Ser Ser Thr
85 90 95Ala Thr Gly Val Ala His Ala Cys Gly His Asp Val His Thr Val Ile
100 105 110Ala Leu Ala Leu Ala Cys Ala Leu Asn Thr Ile Glu Leu Pro Ile Gly
115 120 125Ile Arg Val Ile Phe Gln Pro Ala Glu Glu Val Met Thr Gly Gly Ala
130 135 140Thr Asp Val Ile Ala His Gly Gly Leu Asp Gly Val Asp Ala Ile Tyr145 150 155 160Ala Ile His Val Glu Pro Lys Leu Lys Val Gly Arg Val Gly Val Arg
165 170 175Ala Gly Ala Ile Thr Ser Ala Ser Asp Val Ile Glu Ile Arg Val Lys
180 185 190Gly Glu Gly Gly His Ser Ala Arg Pro His Leu Ser Ala Asp Val Val
195 200 205Tyr Ala Leu Ser Lys Leu Val Val Asp Leu Pro Gly Leu Leu Ser Arg
210 215 220Arg Val Asp Pro Arg Thr Gly Thr Val Leu Val Phe Gly Thr Ile Asn225 230 235 240Ala Gly Tyr Ala Pro Asn Ala Ile Pro Asp Ser Gly Ile Val Ser Gly
245 250 255Thr Leu Arg Thr Ala Asp Ile Ser Thr Trp Arg Asp Met Arg Pro Leu
260 265 270Ile Ser Glu Leu Val Glu Gln Val Leu Ala Pro Thr Gly Val Thr His
275 280 285Glu Leu Ile Tyr Asn Pro Gly Val Pro Pro Val Leu Asn Asp Asp Val
290 295 300Ala Thr Ala Leu Leu Ala Ser Ala Ala Arg Asp Met Asp Thr Gln Ser305 310 315 320Val Val Gln Ala Pro Gln Ser Ser Gly Gly Glu Asp Phe Ser Trp Tyr
325 330 335Leu Glu His Val Pro Gly Ser Met Ala Arg Leu Gly Cys Trp Pro Gly
340 345 350His Gly Pro Lys Gln Asp Leu His Gln Ser Asp Leu Val Val Asp Glu
355 360 365Arg Ala Ile Gly Val Gly Val Arg Leu Phe Gly Ser Leu Val Gln Gln
370 375 380Tyr Ser Ser Arg Ser Glu Ala Phe Leu Asn Ser385 390 395<210>209<211>1308<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1285)<223>FRXA02855<400>209accccgatcc tttgttttcg tgggatcact attagactcg actctaccgc gctgcaggtt 60ttcctgatac gcctgcggac aaaacagaaa ggtatttcac gtg atg gaa att ggt 115
Val Met Glu Ile Gly
1 5gtg cag gtt gcc tca tgg atg gac cgc cac cat gac gag gtc ata aag 163Val Gln Val Ala Ser Trp Met Asp Arg His His Asp Glu Val Ile Lys
10 15 20tgg cgc agg cat ttg cac agc cat cct gag ctc tcc cac atg gaa tac 211Trp Arg Arg His Leu His Ser His Pro Glu Leu Ser His Met Glu Tyr
25 30 35cgc acg act gag tat ttg gcc tcg gtt ctg aaa gat cac ggc atg gaa 259Arg Thr Thr Glu Tyr Leu Ala Ser Val Leu Lys Asp His Gly Met Glu
40 45 50cca cac ctg ttc cca gga acc ggt ttg atg gtg gat atc gga cca gaa 307Pro His Leu Phe Pro Gly Thr Gly Leu Met Val Asp Ile Gly Pro Glu
55 60 65ggg gac tcc cgc ctg gcg ttt cgc gct gat atc gat gcc ctt ccg ctg 355Gly Asp Ser Arg Leu Ala Phe Arg Ala Asp Ile Asp Ala Leu Pro Leu70 75 80 85ctt gaa tca acc ggc tta gag ttc tct tcc aca gcc act ggc gtt gcg 403Leu Glu Ser Thr Gly Leu Glu Phe Ser Ser Thr Ala Thr Gly Val Ala
90 95 100cat gcc tgc gga cat gac gtg cac acg gtg atc gct ttg gca ctt gcc 451His Ala Cys Gly His Asp Val His Thr Val Ile Ala Leu Ala Leu Ala
105 110 115tgt gca ctg aac acc atc gaa ctg ccc atc ggc att cgg gtg att ttc 499Cys Ala Leu Asn Thr Ile Glu Leu Pro Ile Gly Ile Arg Val Ile Phe
120 125 130cag ccg gca gaa gaa gtc atg act ggt ggc gca acg gac gtc att gcc 547Gln Pro Ala Glu Glu Val Met Thr Gly Gly Ala Thr Asp Val Ile Ala
135 140 145cac ggt ggc ctt gat ggt gtg gat gcg att tac gcc atc cac gtt gaa 595His Gly Gly Leu Asp Gly Val Asp Ala Ile Tyr Ala Ile His Val Glu150 155 160 165ccc aaa ttg aag gtc ggt cgc gtc ggt gta cgc gct ggc gcg att act 643Pro Lys Leu Lys Val Gly Arg Val Gly Val Arg Ala Gly Ala Ile Thr
170 175 180tct gcc tca gat gtg atc gaa atc aga gtc aag ggt gaa gga gga cat 691Ser Ala Ser Asp Val Ile Glu Ile Arg Val Lys Gly Glu Gly Gly His
185 190 195agc gca cgt cca cac ctc tcc gct gat gtt gtt tac gcc ttg agc aaa 739Ser Ala Arg Pro His Leu Ser Ala Asp Val Val Tyr Ala Leu Ser Lys
200 205 210ttg gtc gtt gat ctt ccc ggt ttg ctg tcc agg cgc gtc gat cca cgc 787Leu Val Val Asp Leu Pro Gly Leu Leu Ser Arg Arg Val Asp Pro Arg
215 220 225acc ggc acc gtg ctt gtt ttc ggc acc atc aac gcc ggc tat gcg ccc 835Thr Gly Thr Val Leu Val Phe Gly Thr Ile Asn Ala Gly Tyr Ala Pro230 235 240 245aac gcg atc cca gat tcc ggc atc gtg tca ggc acc ttg cgt aca gcc 883Asn Ala Ile Pro Asp Ser Gly Ile Val Ser Gly Thr Leu Arg Thr Ala
250 255 260gac atc tct acc tgg cgt gac atg cgt ccg ctt atc tct gag ctg gtg 931Asp Ile Ser Thr Trp Arg Asp Met Arg Pro Leu Ile Ser Glu Leu Val
265 270 275gaa cag gtg ctc gca ccc acc gga gtc acc cat gaa ctg atc tac aat 979Glu Gln Val Leu Ala Pro Thr Gly Val Thr His Glu Leu Ile Tyr Asn
280 285 290ccg ggt gtt cca cca gtg ctt aac gac gat gtc gcc acc gct ttg ttg 1027Pro Gly Val Pro Pro Val Leu Asn Asp Asp Val Ala Thr Ala Leu Leu
295 300 305gca agc gca gca cgc gac atg gac aca caa tct gtt gtc caa gcg ccg 1075Ala Ser Ala Ala Arg Asp Met Asp Thr Gln Ser Val Val Gln Ala Pro310 315 320 325cag tca tcc ggt gga gaa gac ttc tcg tgg tac ctt gaa cac gtc cca 1123Gln Ser Ser Gly Gly Glu Asp Phe Ser Trp Tyr Leu Glu His Val Pro
330 335 340gga tca atg gcc cgg ttg ggt tgc tgg ccg ggg cac gga ccc aag caa 1171Gly Ser Met Ala Arg Leu Gly Cys Trp Pro Gly His Gly Pro Lys Gln
345 350 355gac ctc cat caa agt gac ctg gtt gtg gat gag cga gcc atc gga gtt 1219Asp Leu His Gln Ser Asp Leu Val Val Asp Glu Arg Ala Ile Gly Val
360 365 370ggc gtc agg ctc ttt ggc tcc ctt gtg cag cag tac agt agc cga tct 1267Gly Val Arg Leu Phe Gly Ser Leu Val Gln Gln Tyr Ser Ser Arg Ser
375 380 385gaa gct ttc tta aat tcc taatgggggt agtgtgtagg gct 1308Glu Ala Phe Leu Asn Ser390 395<210>210<211>395<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>210Val Met Glu Ile Gly Val Gln Val Ala Ser Trp Met Asp Arg His His1 5 10 15Asp Glu Val Ile Lys Trp Arg Arg His Leu His Ser His Pro Glu Leu
20 25 30Ser His Met Glu Tyr Arg Thr Thr Glu Tyr Leu Ala Ser Val Leu Lys
35 40 45Asp His Gly Met Glu Pro His Leu Phe Pro Gly Thr Gly Leu Met Val
50 55 60Asp Ile Gly Pro Glu Gly Asp Ser Arg Leu Ala Phe Arg Ala Asp Ile65 70 75 80Asp Ala Leu Pro Leu Leu Glu Ser Thr Gly Leu Glu Phe Ser Ser Thr
85 90 95Ala Thr Gly Val Ala His Ala Cys Gly His Asp Val His Thr Val Ile
100 105 110Ala Leu Ala Leu Ala Cys Ala Leu Asn Thr Ile Glu Leu Pro Ile Gly
115 120 125Ile Arg Val Ile Phe Gln Pro Ala Glu Glu Val Met Thr Gly Gly Ala
130 135 140Thr Asp Val Ile Ala His Gly Gly Leu Asp Gly Val Asp Ala Ile Tyr145 150 155 160Ala Ile His Val Glu Pro Lys Leu Lys Val Gly Arg Val Gly Val Arg
165 170 175Ala Gly Ala Ile Thr Ser Ala Ser Asp Val Ile Glu Ile Arg Val Lys
180 185 190Gly Glu Gly Gly His Ser Ala Arg Pro His Leu Ser Ala Asp Val Val
195 200 205Tyr Ala Leu Ser Lys Leu Val Val Asp Leu Pro Gly Leu Leu Ser Arg
210 215 220Arg Val Asp Pro Arg Thr Gly Thr Val Leu Val Phe Gly Thr Ile Asn225 230 235 240Ala Gly Tyr Ala Pro Asn Ala Ile Pro Asp Ser Gly Ile Val Ser Gly
245 250 255Thr Leu Arg Thr Ala Asp Ile Ser Thr Trp Arg Asp Met Arg Pro Leu
260 265 270Ile Ser Glu Leu Val Glu Gln Val Leu Ala Pro Thr Gly Val Thr His
275 280 285Glu Leu Ile Tyr Asn Pro Gly Val Pro Pro Val Leu Asn Asp Asp Val
290 295 300Ala Thr Ala Leu Leu Ala Ser Ala Ala Arg Asp Met Asp Thr Gln Ser305 310 315 320Val Val Gln Ala Pro Gln Ser Ser Gly Gly Glu Asp Phe Ser Trp Tyr
325 330 335Leu Glu His Val Pro Gly Ser Met Ala Arg Leu Gly Cys Trp Pro Gly
340 345 350His Gly Pro Lys Gln Asp Leu His Gln Ser Asp Leu Val Val Asp Glu
355 360 365Arg Ala Ile Gly Val Gly Val Arg Leu Phe Gly Ser Leu Val Gln Gln
370 375 380Tyr Ser Ser Arg Ser Glu Ala Phe Leu Asn Ser385 390 395<210>211<211>1509<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1486)<223>RXA00026<400>211ccctttctgg ctagcctggg ctacattgtt ggcaatttgg ttctacgccg atttgccgct 60tggacctggc tctgcgatct tcctcgaagg ataagttttc atg agt act gac aat 115
Met Ser Thr Asp Asn
1 5ttt tct cca caa gtt ccg tcg act gtg tat ttg gat tac atg gag caa 163Phe Ser Pro Gln Val Pro Ser Thr Val Tyr Leu Asp Tyr Met Glu Gln
10 15 20ggg att gcc gcg cgc aaa gcg gag gca gaa tct aac gcc agc acg aag 211Gly Ile Ala Ala Arg Lys Ala Glu Ala Glu Ser Asn Ala Ser Thr Lys
25 30 35ggg gag agc ccg gat tat cca ggc cag cag gtt att tgg cgc ctg atc 259Gly Glu Ser Pro Asp Tyr Pro Gly Gln Gln Val Ile Trp Arg Leu Ile
40 45 50cag gaa gca ggg gag tcg ttg cgt gat gaa ctg cgc aca ctg gct ttc 307Gln Glu Ala Gly Glu Ser Leu Arg Asp Glu Leu Arg Thr Leu Ala Phe
55 60 65acg ctg cac gac cat ccg gaa gaa gcg ttc gag gag gtg ttc gcc acc 355Thr Leu His Asp His Pro Glu Glu Ala Phe Glu Glu Val Phe Ala Thr70 75 80 85gag gaa atc aca aaa ctt ctg caa aat cat ggt ttt gag gtt cag agt 403Glu Glu Ile Thr Lys Leu Leu Gln Asn His Gly Phe Glu Val Gln Ser
90 95 100gga gtt tat ggt gtt aaa acc gct cta gaa act agt ttt gaa acc cct 451Gly Val Tyr Gly Val Lys Thr Ala Leu Glu Thr Ser Phe Glu Thr Pro
105 110 115ggt tat gat cca gcg cag cac cca agc att gcg atc ttg gcg gaa tac 499Gly Tyr Asp Pro Ala Gln His Pro Ser Ile Ala Ile Leu Ala Glu Tyr
120 125 130gat gcc ctt cca gag atc ggc cat gca tgc ggg cac aat atc atc gca 547Asp Ala Leu Pro Glu Ile Gly His Ala Cys Gly His Asn Ile Ile Ala
135 140 145gca gct ggt gtt ggc gca ttt tta gct gtc acc aac atg atc aaa act 595Ala Ala Gly Val Gly Ala Phe Leu Ala Val Thr Asn Met Ile Lys Thr150 155 160 165gcc gaa gtg aaa ggc gtg gat cac ctc gac ttt gaa ggc cgg atc gtg 643Ala Glu Val Lys Gly Val Asp His Leu Asp Phe Glu Gly Arg Ile Val
170 175 180ctg ttg gga aca cct gct gag gag ggg cat tcc ggc aag gaa tac atg 691Leu Leu Gly Thr Pro Ala Glu Glu Gly His Ser Gly Lys Glu Tyr Met
185 190 195atc cga aat ggc gca ttc gat ggc att gat gcg tcg att atg atg cac 739Ile Arg Asn Gly Ala Phe Asp Gly Ile Asp Ala Ser Ile Met Met His
200 205 210ccc ttt ggc ttc gat ctg gcg gag cat gtt tgg gtg ggc aga cgt acc 787Pro Phe Gly Phe Asp Leu Ala Glu His Val Trp Val Gly Arg Arg Thr
215 220 225atg acg gcg acg ttc cac ggt gtc tct gca cac gcg tct tcg cag cct 835Met Thr Ala Thr Phe His Gly Val Ser Ala His Ala Ser Ser Gln Pro230 235 240 245ttc atg ggt aaa aat gcc ctc gac gct gca agt ttg gcg tac cag ggc 883Phe Met Gly Lys Asn Ala Leu Asp Ala Ala Ser Leu Ala Tyr Gln Gly
250 255 260ttc gga gtt ttg cgt cag caa atg cca ccg agc gac cgc ctt cac gcc 931Phe Gly Val Leu Arg Gln Gln Met Pro Pro Ser Asp Arg Leu His Ala
265 270 275att att acg gaa ggc gga aac cgg cca agc atc att cca gac act gca 979Ile Ile Thr Glu Gly Gly Asn Arg Pro Ser Ile Ile Pro Asp Thr Ala
280 285 290acg atg tcg ctg tac gtg cgt tct ttg ttg ccg gaa gca ctc aaa gac 1027Thr Met Ser Leu Tyr Val Arg Ser Leu Leu Pro Glu Ala Leu Lys Asp
295 300 305ata tcg aaa cgc gtg gat gat gtg ctc gat ggg gcg gcc ttg atg gcg 1075Ile Ser Lys Arg Val Asp Asp Val Leu Asp Gly Ala Ala Leu Met Ala310 315 320 325ggg gtt ggc gtc gaa aag caa tgg gat gtg cac cca gct agc ttg ccc 1123Gly Val Gly Val Glu Lys Gln Trp Asp Val His Pro Ala Ser Leu Pro
330 335 340gtg cgc aac aat cat gtg ttg gcg cgg cgt tgg gca aaa acg cag aat 1171Val Arg Asn Asn His Val Leu Ala Arg Arg Trp Ala Lys Thr Gln Asn
345 350 355ctg cgt ggt cga acg gcg ctt tcg gag ggt att ttg ccc gac act ctg 1219Leu Arg Gly Arg Thr Ala Leu Ser Glu Gly Ile Leu Pro Asp Thr Leu
360 365 370gca gca tcg act gat ttt ggc aat gtc tcg cac ctg gtt ccg ggc att 1267Ala Ala Ser Thr Asp Phe Gly Asn Val Ser His Leu Val Pro Gly Ile
375 380 385cat ccg atg gtg aaa att tct ccg gaa aac gtt gcg ctc cac acc aag 1315His Pro Met Val Lys Ile Ser Pro Glu Asn Val Ala Leu His Thr Lys390 395 400 405gaa ttc gcc gct tat gcg cgc acg gaa gag gcc atc gac gca gcc gtc 1363Glu Phe Ala Ala Tyr Ala Arg Thr Glu Glu Ala Ile Asp Ala Ala Val
410 415 420gac gcc gca atc ggg ctg gcg caa gtc gcc gtt gac gcg ctt gca gat 1411Asp Ala Ala Ile Gly Leu Ala Gln Val Ala Val Asp Ala Leu Ala Asp
425 430 435ccg caa atg ctt atc gac gcg acc ctc gag ttc acc aac tcc ggc gac 1459Pro Gln Met Leu Ile Asp Ala Thr Leu Glu Phe Thr Asn Ser Gly Asp
440 445 450gta ctt aaa gta ggg gac tat ttg gct taggcaacga ctccgaaacc 1506Val Leu Lys Val Gly Asp Tyr Leu Ala
455 460ttc1509<210>212<211>462<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>212Met Ser Thr Asp Asn Phe Ser Pro Gln Val Pro Ser Thr Val Tyr Leu1 5 10 15Asp Tyr Met Glu Gln Gly Ile Ala Ala Arg Lys Ala Glu Ala Glu Ser
20 25 30Asn Ala Ser Thr Lys Gly Glu Ser Pro Asp Tyr Pro Gly Gln Gln Val
35 40 45Ile Trp Arg Leu Ile Gln Glu Ala Gly Glu Ser Leu Arg Asp Glu Leu
50 55 60Arg Thr Leu Ala Phe Thr Leu His Asp His Pro Glu Glu Ala Phe Glu65 70 75 80Glu Val Phe Ala Thr Glu Glu Ile Thr Lys Leu Leu Gln Asn His Gly
85 90 95Phe Glu Val Gln Ser Gly Val Tyr Gly Val Lys Thr Ala Leu Glu Thr
100 105 110Ser Phe Glu Thr Pro Gly Tyr Asp Pro Ala Gln His Pro Ser Ile Ala
115 120 125Ile Leu Ala Glu Tyr Asp Ala Leu Pro Glu Ile Gly His Ala Cys Gly
130 135 140His Asn Ile Ile Ala Ala Ala Gly Val Gly Ala Phe Leu Ala Val Thr145 150 155 160Asn Met Ile Lys Thr Ala Glu Val Lys Gly Val Asp His Leu Asp Phe
165 170 175Glu Gly Arg Ile Val Leu Leu Gly Thr Pro Ala Glu Glu Gly His Ser
180 185 190Gly Lys Glu Tyr Met Ile Arg Asn Gly Ala Phe Asp Gly Ile Asp Ala
195 200 205Ser Ile Met Met His Pro Phe Gly Phe Asp Leu Ala Glu His Val Trp
210 215 220Val Gly Arg Arg Thr Met Thr Ala Thr Phe His Gly Val Ser Ala His225 230 235 240Ala Ser Ser Gln Pro Phe Met Gly Lys Asn Ala Leu Asp Ala Ala Ser
245 250 255Leu Ala Tyr Gln Gly Phe Gly Val Leu Arg Gln Gln Met Pro Pro Ser
260 265 270Asp Arg Leu His Ala Ile Ile Thr Glu Gly Gly Asn Arg Pro Ser Ile
275 280 285Ile Pro Asp Thr Ala Thr Met Ser Leu Tyr Val Arg Ser Leu Leu Pro
290 295 300Glu Ala Leu Lys Asp Ile Ser Lys Arg Val Asp Asp Val Leu Asp Gly305 310 315 320Ala Ala Leu Met Ala Gly Val Gly Val Glu Lys Gln Trp Asp Val His
325 330 335Pro Ala Ser Leu Pro Val Arg Asn Asn His Val Leu Ala Arg Arg Trp
340 345 350Ala Lys Thr Gln Asn Leu Arg Gly Arg Thr Ala Leu Ser Glu Gly Ile
355 360 365Leu Pro Asp Thr Leu Ala Ala Ser Thr Asp Phe Gly Asn Val Ser His
370 375 380Leu Val Pro Gly Ile His Pro Met Val Lys Ile Ser Pro Glu Asn Val385 390 395 400Ala Leu His Thr Lys Glu Phe Ala Ala Tyr Ala Arg Thr Glu Glu Ala
405 410 415Ile Asp Ala Ala Val Asp Ala Ala Ile Gly Leu Ala Gln Val Ala Val
420 425 430Asp Ala Leu Ala Asp Pro Gln Met Leu Ile Asp Ala Thr Leu Glu Phe
435 440 445Thr Asn Ser Gly Asp Val Leu Lys Val Gly Asp Tyr Leu Ala
450 455 460<210>213<211>954<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(931)<223>RXA01971<400>213aggtcttgtt tatttcggct actgattcag tagctgcgct ccgataggat tcttagtttt 60cagttcagta tctttgagcc acggctagaa tgtgaatcct atg tct aag aag aag 115
Met Ser Lys Lys Lys
1 5cct cgc ccc att ccg gtt cct gcc caa ttt atc cct ggt ctc att gat 163Pro Arg Pro Ile Pro Val Pro Ala Gln Phe Ile Pro Gly Leu Ile Asp
10 15 20gcg cat aca cat ttg gca tcg tgt gga gga gat ctt gca ggg ttg gtg 211Ala His Thr His Leu Ala Ser Cys Gly Gly Asp Leu Ala Gly Leu Val
25 30 35gaa agg gcc aag gag gcg ggc gtc gaa aag ctt tgt acc gtc ggt gat 259Glu Arg Ala Lys Glu Ala Gly Val Glu Lys Leu Cys Thr Val Gly Asp
40 45 50ggt ttg gct gag gcc gag ctt gcg ctg gag gcc gcg caa cag ttt ggc 307Gly Leu Ala Glu Ala Glu Leu Ala Leu Glu Ala Ala Gln Gln Phe Gly
55 60 65aat gtg ttt gct gcg tgt gcg att cat ccg acg aag gct gat cag ttg 355Asn Val Phe Ala Ala Cys Ala Ile His Pro Thr Lys Ala Asp Gln Leu70 75 80 85gat ggg gct gcg cgt gcg cgg ctg acg cag atg gcg gcg gat ccg aat 403Asp Gly Ala Ala Arg Ala Arg Leu Thr Gln Met Ala Ala Asp Pro Asn
90 95 100tgt gtg gcc att ggt gag act ggt ttg gat tcg tat tgg atc aag cac 451Cys Val Ala Ile Gly Glu Thr Gly Leu Asp Ser Tyr Trp Ile Lys His
105 110 115gat cca gag gac acg gcg gcg ttg gat gtg caa gag gag gcg ctg cgc 499Asp Pro Glu Asp Thr Ala Ala Leu Asp Val Gln Glu Glu Ala Leu Arg
120 125 130tgg cat att gat ttg gca att agt gcg gat aag ccg ttg atg att cac 547Trp His Ile Asp Leu Ala Ile Ser Ala Asp Lys Pro Leu Met Ile His
135 140 145aat cgt gag gcg gat gct gat ttg atg cga gtg ttg gcg gat gct cca 595Asn Arg Glu Ala Asp Ala Asp Leu Met Arg Val Leu Ala Asp Ala Pro150 155 160 165cct cca aaa gat acg att ctg cat tgt ttt tct tcg ccg ttg gac gtg 643Pro Pro Lys Asp Thr Ile Leu His Cys Phe Ser Ser Pro Leu Asp Val
170 175 180gcg aag gaa gcg ttg gat cgt gga tat gtg ttg agt ttt gcg ggc aat 691Ala Lys Glu Ala Leu Asp Arg Gly Tyr Val Leu Ser Phe Ala Gly Asn
185 190 195gtg acg ttt aag cgt aat gag gag ttg cgg gag gct gct cgt att gcg 739Val Thr Phe Lys Arg Asn Glu Glu Leu Arg Glu Ala Ala Arg Ile Ala
200 205 210ccg att tcc cag att ttg att gaa acc gat gcg ccg tat atg acg ccg 787Pro Ile Ser Gln Ile Leu Ile Glu Thr Asp Ala Pro Tyr Met Thr Pro
215 220 225gag ccg ttt cgg ggg agt agg aat gag ccg tcg ttg att ggt cat acg 835Glu Pro Phe Arg Gly Ser Arg Asn Glu Pro Ser Leu Ile Gly His Thr230 235 240 245gcg cta tgc att gcg gag gtt cgg ggg atg gct gtg gag gat gtt gcg 883Ala Leu Cys Ile Ala Glu Val Arg Gly Met Ala Val Glu Asp Val Ala
250 255 260gcg gct ttg aat gag aat ttt gat cgc gtt tat ggg gtc aca aat cta 931Ala Ala Leu Asn Glu Asn Phe Asp Arg Val Tyr Gly Val Thr Asn Leu
265 270 275taacgtgagg tagctcacag tca 954<210>214<211>277<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>214Met Ser Lys Lys Lys Pro Arg Pro Ile Pro Val Pro Ala Gln Phe Ile1 5 10 15Pro Gly Leu Ile Asp Ala His Thr His Leu Ala Ser Cys Gly Gly Asp
20 25 30Leu Ala Gly Leu Val Glu Arg Ala Lys Glu Ala Gly Val Glu Lys Leu
35 40 45Cys Thr Val Gly Asp Gly Leu Ala Glu Ala Glu Leu Ala Leu Glu Ala
50 55 60Ala Gln Gln Phe Gly Asn Val Phe Ala Ala Cys Ala Ile His Pro Thr65 70 75 80Lys Ala Asp Gln Leu Asp Gly Ala Ala Arg Ala Arg Leu Thr Gln Met
85 90 95Ala Ala Asp Pro Asn Cys Val Ala Ile Gly Glu Thr Gly Leu Asp Ser
100 105 110Tyr Trp Ile Lys His Asp Pro Glu Asp Thr Ala Ala Leu Asp Val Gln
115 120 125Glu Glu Ala Leu Arg Trp His Ile Asp Leu Ala Ile Ser Ala Asp Lys
130 135 140Pro Leu Met Ile His Asn Arg Glu Ala Asp Ala Asp Leu Met Arg Val145 150 155 160Leu Ala Asp Ala Pro Pro Pro Lys Asp Thr Ile Leu His Cys Phe Ser
165 170 175Ser Pro Leu Asp Val Ala Lys Glu Ala Leu Asp Arg Gly Tyr Val Leu
180 185 190Ser Phe Ala Gly Asn Val Thr Phe Lys Arg Asn Glu Glu Leu Arg Glu
195 200 205Ala Ala Arg Ile Ala Pro Ile Ser Gln Ile Leu Ile Glu Thr Asp Ala
210 215 220Pro Tyr Met Thr Pro Glu Pro Phe Arg Gly Ser Arg Asn Glu Pro Ser225 230 235 240Leu Ile Gly His Thr Ala Leu Cys Ile Ala Glu Val Arg Gly Met Ala
245 250 255Val Glu Asp Val Ala Ala Ala Leu Asn Glu Asn Phe Asp Arg Val Tyr
260 265 270Gly Val Thr Asn Leu
275<210>215<211>954<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(931)<223>RXA01802<400>215ggaattctgg acaaaagtgt tcactacgtt agacatgaga accagtgtgg cacatcacag 60gaaatcttcg cgggtgttta gacaacccgg atgtgacaga atg ggc gat caa gac 115
Met Gly Asp Gln Asp
1 5ata atc gga aag gaa tcc aaa caa atg gac ttt cgc ctc gtc gcg aca 163Ile Ile Gly Lys Glu Ser Lys Gln Met Asp Phe Arg Leu Val Ala Thr
10 15 20gac atg gac ggc aca ctt tta aac acc cac cac gaa gtc cca gag aaa 211Asp Met Asp Gly Thr Leu Leu Asn Thr His His Glu Val Pro Glu Lys
25 30 35ttt tgg gac atc ctg gaa caa atg cgt gcc aaa gga atc gcc ttc gca 259Phe Trp Asp Ile Leu Glu Gln Met Arg Ala Lys Gly Ile Ala Phe Ala
40 45 50cca gcc agc ggc cgt caa tta gcc acc ttg caa aaa caa ttc ggg cac 307Pro Ala Ser Gly Arg Gln Leu Ala Thr Leu Gln Lys Gln Phe Gly His
55 60 65gcg ggt gaa ccc att tct tac atc gca gaa aac ggc acc gtg gta gtc 355Ala Gly Glu Pro Ile Ser Tyr Ile Ala Glu Asn Gly Thr Val Val Val70 75 80 85cac gac ggc gaa att atc tcc ctg acc acc atc gac tcc gac acc gta 403His Asp Gly Glu Ile Ile Ser Leu Thr Thr Ile Asp Ser Asp Thr Val
90 95 100cac tcc atc atc gat gcc gtg cgc gca tcc gac atc gat atg gga gta 451His Ser Ile Ile Asp Ala Val Arg Ala Ser Asp Ile Asp Met Gly Val
105 110 115gtg gtc tgc cga cca gaa cgc gcc tac gtc gaa cgc aac gac gaa gct 499Val Val Cys Arg Pro Glu Arg Ala Tyr Val Glu Arg Asn Asp Glu Ala
120 125 130ttc cgc gcc gaa ggc ctg aaa tac tac gtc tcc atc gag gaa gtc caa 547Phe Arg Ala Glu Gly Leu Lys Tyr Tyr Val Ser Ile Glu Glu Val Gln
135 140 145gac ctc cac gaa gca gtc aac aat gaa gta atc aag gta gcg atc ttt 595Asp Leu His Glu Ala Val Asn Asn Glu Val Ile Lys Val Ala Ile Phe150 155 160 165aca ttc caa gat gcc gaa aag gac tgt gcc ccc atc atc cgc gca gcc 643Thr Phe Gln Asp Ala Glu Lys Asp Cys Ala Pro Ile Ile Arg Ala Ala
170 175 180tcc ccc aac gcc aac gtt gtt gtc tcc ggc cag cac tgg gtc gat gtc 691Ser Pro Asn Ala Asn Val Val Val Ser Gly Gln His Trp Val Asp Val
185 190 195atg gat cct tca gcc aac aag ggc caa gct ttg gct gct ctc cgc gat 739Met Asp Pro Ser Ala Asn Lys Gly Gln Ala Leu Ala Ala Leu Arg Asp
200 205 210gcc ctc gga ttg gaa gaa tcc caa act ctc gtg ttt ggc gac tac ctc 787Ala Leu Gly Leu Glu Glu Ser Gln Thr Leu Val Phe Gly Asp Tyr Leu
215 220 225aac gac act gaa ttg atc aag gcc gcc ggc aag tct tac gcc atg tcc 835Asn Asp Thr Glu Leu Ile Lys Ala Ala Gly Lys Ser Tyr Ala Met Ser230 235 240 245aat gcc cac ccg gac att ttg gaa ttg gcc gac gaa att gca cca tcc 883Asn Ala His Pro Asp Ile Leu Glu Leu Ala Asp Glu Ile Ala Pro Ser
250 255 260aac att gaa gag ggc gtt att gtg gtg ctg gag aag ttg ctt aac ggt 931Asn Ile Glu Glu Gly Val Ile Val Val Leu Glu Lys Leu Leu Asn Gly
265 270 275taacgattgc aggcagcagg ttc 954<210>216<211>277<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>216Met Gly Asp Gln Asp Ile Ile Gly Lys Glu Ser Lys Gln Met Asp Phe1 5 10 15Arg Leu Val Ala Thr Asp Met Asp Gly Thr Leu Leu Asn Thr His His
20 25 30Glu Val Pro Glu Lys Phe Trp Asp Ile Leu Glu Gln Met Arg Ala Lys
35 40 45Gly Ile Ala Phe Ala Pro Ala Ser Gly Arg Gln Leu Ala Thr Leu Gln
50 55 60Lys Gln Phe Gly His Ala Gly Glu Pro Ile Ser Tyr Ile Ala Glu Asn65 70 75 80Gly Thr Val Val Val His Asp Gly Glu Ile Ile Ser Leu Thr Thr Ile
85 90 95Asp Ser Asp Thr Val His Ser Ile Ile Asp Ala Val Arg Ala Ser Asp
100 105 110Ile Asp Met Gly Val Val Val Cys Arg Pro Glu Arg Ala Tyr Val Glu
115 120 125Arg Asn Asp Glu Ala Phe Arg Ala Glu Gly Leu Lys Tyr Tyr Val Ser
130 135 140Ile Glu Glu Val Gln Asp Leu His Glu Ala Val Asn Asn Glu Val Ile145 150 155 160Lys Val Ala Ile Phe Thr Phe Gln Asp Ala Glu Lys Asp Cys Ala Pro
165 170 175Ile Ile Arg Ala Ala Ser Pro Asn Ala Asn Val Val Val Ser Gly Gln
180 185 190His Trp Val Asp Val Met Asp Pro Ser Ala Asn Lys Gly Gln Ala Leu
195 200 205Ala Ala Leu Arg Asp Ala Leu Gly Leu Glu Glu Ser Gln Thr Leu Val
210 215 220Phe Gly Asp Tyr Leu Asn Asp Thr Glu Leu Ile Lys Ala Ala Gly Lys225 230 235 240Ser Tyr Ala Met Ser Asn Ala His Pro Asp Ile Leu Glu Leu Ala Asp
245 250 255Glu Ile Ala Pro Ser Asn Ile Glu Glu Gly Val Ile Val Val Leu Glu
260 265 270Lys Leu Leu Asn Gly
275<210>217<211>1066<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1066)<223>RXN00866<400>217gcatcaacgt aggagatcct cgacttccaa ttatggctcc aaatgagcag gaacttgagg 60ctctccgaga agacatgaaa aaagctggag ttctataaat atg aat gat tcc cga 115
Met Asn Asp Ser Arg
1 5aat cgc ggc cgg aag gtt acc cgc aag gcg ggc cca cca gaa gct ggt 163Asn Arg Gly Arg Lys Val Thr Arg Lys Ala Gly Pro Pro Glu Ala Gly
10 15 20cag gaa aac cat ctg gat acc cct gtc ttt cag gca cca gat gct tcc 211Gln Glu Asn His Leu Asp Thr Pro Val Phe Gln Ala Pro Asp Ala Ser
25 30 35tct aac cag agc gct gta aaa gct gag acc gcc gga aac gac aat cgg 259Ser Asn Gln Ser Ala Val Lys Ala Glu Thr Ala Gly Asn Asp Asn Arg
40 45 50gat gct gcg caa ggt gct caa gga tcc caa gat tct cag ggt tcc cag 307Asp Ala Ala Gln Gly Ala Gln Gly Ser Gln Asp Ser Gln Gly Ser Gln
55 60 65aac gct caa ggt tcc cag aac cgc gag tcc gga aac aac aac cgc aac 355Asn Ala Gln Gly Ser Gln Asn Arg Glu Ser Gly Asn Asn Asn Arg Asn70 75 80 85cgt tcc aac aac aac cgt cgc ggt ggt cgt gga cgt cgt gga tcc gga 403Arg Ser Asn Asn Asn Arg Arg Gly Gly Arg Gly Arg Arg Gly Ser Gly
90 95 100aac gcc aat gag ggc gcg aac aac aac agc ggt aac cag aac cgt cag 451Asn Ala Asn Glu Gly Ala Asn Asn Asn Ser Gly Asn Gln Asn Arg Gln
105 110 115ggc gga aac cgt ggc aac cgc ggt ggc gga cgc cga aac gtt gtt aag 499Gly Gly Asn Arg Gly Asn Arg Gly Gly Gly Arg Arg Asn Val Val Lys
120 125 130tcg atg cag ggt gcg gat ctg acc cag cgc ctg cca gag cca cca aag 547Ser Met Gln Gly Ala Asp Leu Thr Gln Arg Leu Pro Glu Pro Pro Lys
135 140 145gca ccg gca aac ggt ctg cgt att tac gca ctt ggt ggc att tcc gaa 595Ala Pro Ala Asn Gly Leu Arg Ile Tyr Ala Leu Gly Gly Ile Ser Glu150 155 160 165atc ggt cgc aac atg acc gtg ttt gag tac aac aac cgt ctg ctc atc 643Ile Gly Arg Asn Met Thr Val Phe Glu Tyr Asn Asn Arg Leu Leu Ile
170 175 180gtg gac tgt ggt gtg ctc ttc cca tct tca ggt gag cca ggc gtt gac 691Val Asp Cys Gly Val Leu Phe Pro Ser Ser Gly Glu Pro Gly Val Asp
185 190 195ctg att ctt cct gac ttc ggc cca att gag gat cac ctg cac cgc gtc 739Leu Ile Leu Pro Asp Phe Gly Pro Ile Glu Asp His Leu His Arg Val
200 205 210gat gca ttg gtg gtt act cac gga cac gaa gac cac att ggt gct att 787Asp Ala Leu Val Val Thr His Gly His Glu Asp His Ile Gly Ala Ile
215 220 225ccc tgg ctg ctg aag ctg cgc aac gat atc cca atc ttg gca tcc cgt 835Pro Trp Leu Leu Lys Leu Arg Asn Asp Ile Pro Ile Leu Ala Ser Arg230 235 240 245ttc acc ttg gct ctg att gca gct aag tgt aag gaa cac cgt cag cgt 883Phe Thr Leu Ala Leu Ile Ala Ala Lys Cys Lys Glu His Arg Gln Arg
250 255 260ccg aag ctg atc gag gtc aac gag cag tcc aat gag gac cgc gga ccg 931Pro Lys Leu Ile Glu Val Asn Glu Gln Ser Asn Glu Asp Arg Gly Pro
265 270 275ttc aac att cgc ttc tgg gct gtt aac cac tcc atc cca gac tgc ctt 979Phe Asn Ile Arg Phe Trp Ala Val Asn His Ser Ile Pro Asp Cys Leu
280 285 290ggt ctt gct atc aag act cct gct ggt ttg gtc atc cac acc ggt gac 1027Gly Leu Ala Ile Lys Thr Pro Ala Gly Leu Val Ile His Thr Gly Asp
295 300 305atc aag ctg gat cag act cct cct gat gga cgc cca act 1066Ile Lys Leu Asp Gln Thr Pro Pro Asp Gly Arg Pro Thr310 315 320<210>218<211>322<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>218Met Asn Asp Ser Arg Asn Arg Gly Arg Lys Val Thr Arg Lys Ala Gly1 5 10 15Pro Pro Glu Ala Gly Gln Glu Asn His Leu Asp Thr Pro Val Phe Gln
20 25 30Ala Pro Asp Ala Ser Ser Asn Gln Ser Ala Val Lys Ala Glu Thr Ala
35 40 45Gly Asn Asp Asn Arg Asp Ala Ala Gln Gly Ala Gln Gly Ser Gln Asp
50 55 60Ser Gln Gly Ser Gln Asn Ala Gln Gly Ser Gln Asn Arg Glu Ser Gly65 70 75 80Asn Asn Asn Arg Asn Arg Ser Asn Asn Asn Arg Arg Gly Gly Arg Gly
85 90 95Arg Arg Gly Ser Gly Asn Ala Asn Glu Gly Ala Asn Asn Asn Ser Gly
100 105 110Asn Gln Asn Arg Gln Gly Gly Asn Arg Gly Asn Arg Gly Gly Gly Arg
115 120 125Arg Asn Val Val Lys Ser Met Gln Gly Ala Asp Leu Thr Gln Arg Leu
130 135 140Pro Glu Pro Pro Lys Ala Pro Ala Asn Gly Leu Arg Ile Tyr Ala Leu145 150 155 160Gly Gly Ile Ser Glu Ile Gly Arg Asn Met Thr Val Phe Glu Tyr Asn
165 170 175Asn Arg Leu Leu Ile Val Asp Cys Gly Val Leu Phe Pro Ser Ser Gly
180 185 190Glu Pro Gly Val Asp Leu Ile Leu Pro Asp Phe Gly Pro Ile Glu Asp
195 200 205His Leu His Arg Val Asp Ala Leu Val Val Thr His Gly His Glu Asp
210 215 220His Ile Gly Ala Ile Pro Trp Leu Leu Lys Leu Arg Asn Asp Ile Pro225 230 235 240Ile Leu Ala Ser Arg Phe Thr Leu Ala Leu Ile Ala Ala Lys Cys Lys
245 250 255Glu His Arg Gln Arg Pro Lys Leu Ile Glu Val Asn Glu Gln Ser Asn
260 265 270Glu Asp Arg Gly Pro Phe Asn Ile Arg Phe Trp Ala Val Asn His Ser
275 280 285Ile Pro Asp Cys Leu Gly Leu Ala Ile Lys Thr Pro Ala Gly Leu Val
290 295 300Ile His Thr Gly Asp Ile Lys Leu Asp Gln Thr Pro Pro Asp Gly Arg305 310 315 320Pro Thr<210>219<211>1045<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1045)<223>FRXA00866<400>219gcatcaacgt aggagatcct cgacttccaa ttatggctcc aaatgagcag gaacttgagg 60ctctccgaga agacatgaaa aaagctggag ttctataaat atg aat gat tcc cga 115
Met Asn Asp Ser Arg
1 5aat cgc ggc cgg aag gtt acc cgc aag gcg ggc cca cca gaa gct ggt 163Asn Arg Gly Arg Lys ValThr Arg Lys Ala Gly Pro Pro Glu Ala Gly
10 15 20cag gaa aac cat ctg gat acc cct gtc ttt cag gca cca gat gct tcc 211Gln Glu Asn His Leu Asp Thr Pro Val Phe Gln Ala Pro Asp Ala Ser
25 30 35tct aac cag agc gct gta aaa gct gag acc gcc gga aac gac aat cgg 259Ser Asn Gln Ser Ala Val Lys Ala Glu Thr Ala Gly Asn Asp Asn Arg
40 45 50gat gct gcg caa ggt gct caa gga tcc caa gat tct cag ggt tcc cag 307Asp Ala Ala Gln Gly Ala Gln Gly Ser Gln Asp Ser Gln Gly Ser Gln
55 60 65aac gct caa ggt tcc cag aac cgc gag tcc gga aac aac aac cgc aac 355Asn Ala Gln Gly Ser Gln Asn Arg Glu Ser Gly Asn Asn Asn Arg Asn70 75 80 85cgt tcc aac aac aac cgt cgc ggt ggt cgt gga cgt cgt gga tcc gga 403Arg Ser Asn Asn Asn Arg Arg Gly Gly Arg Gly Arg Arg Gly Ser Gly
90 95 100aac gcc aat gag ggc gcg aac aac aac agc ggt aac cag aac cgt cag 451Asn Ala Asn Glu Gly Ala Asn Asn Asn Ser Gly Asn Gln Asn Arg Gln
105 110 115ggc gga aac cgt ggc aac cgc ggt ggc gga cgc cga aac gtt gtt aag 499Gly Gly Asn Arg Gly Asn Arg Gly Gly Gly Arg Arg Asn Val Val Lys
120 125 130tcg atg cag ggt gcg gat ctg acc cag cgc ctg cca gag cca cca aag 547Ser Met Gln Gly Ala Asp Leu Thr Gln Arg Leu Pro Glu Pro Pro Lys
135 140 145gca ccg gca aac ggt ctg cgt att tac gca ctt ggt ggc att tcc gaa 595Ala Pro Ala Asn Gly Leu Arg Ile Tyr Ala Leu Gly Gly Ile Ser Glu150 155 160 165atc ggt cgc aac atg acc gtg ttt gag tac aac aac cgt ctg ctc atc 643Ile Gly Arg Asn Met Thr Val Phe Glu Tyr Asn Asn Arg Leu Leu Ile
170 175 180gtg gac tgt ggt gtg ctc ttc cca tct tca ggt gag cca ggc gtt gac 691Val Asp Cys Gly Val Leu Phe Pro Ser Ser Gly Glu Pro Gly Val Asp
185 190 195ctg att ctt cct gac ttc ggc cca att gag gat cac ctg cac cgc gtc 739Leu Ile Leu Pro Asp Phe Gly Pro Ile Glu Asp His Leu His Arg Val
200 205 210gat gca ttg gtg gtt act cac gga cac gaa gac cac att ggt gct att 787Asp Ala Leu Val Val Thr His Gly His Glu Asp His Ile Gly Ala Ile
215 220 225ccc tgg ctg ctg aag ctg cgc aac gat atc cca atc ttg gca tcc cgt 835Pro Trp Leu Leu Lys Leu Arg Asn Asp Ile Pro Ile Leu Ala Ser Arg230 235 240 245ttc acc ttg gct ctg att gca gct aag tgt aag gaa cac cgt cag cgt 883Phe Thr Leu Ala Leu Ile Ala Ala Lys Cys Lys Glu His Arg Gln Arg
250 255 260ccg aag ctg atc gag gtc aac gag cag tcc aat gag gac cgc gga ccg 931Pro Lys Leu Ile Glu Val Asn Glu Gln Ser Asn Glu Asp Arg Gly Pro
265 270 275ttc aac att cgc ttc tgg gct gtt aac cac tcc atc cca gac tgc ctt 979Phe Asn Ile Arg Phe Trp Ala Val Asn His Ser Ile Pro Asp Cys Leu
280 285 290ggt ctt gct atc aag act cct gct ggt ttg gtc atc cac acc ggt gac 1027Gly Leu Ala Ile Lys Thr Pro Ala Gly Leu Val Ile His Thr Gly Asp
295 300 305atc aag ctg gat cag act 1045Ile Lys Leu Asp Gln Thr310 315<210>220<211>315<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>220Met Asn Asp Ser Arg Asn Arg Gly Arg Lys Val Thr Arg Lys Ala Gly1 5 10 15Pro Pro Glu Ala Gly Gln Glu Asn His Leu Asp Thr Pro Val Phe Gln
20 25 30Ala Pro Asp Ala Ser Ser Asn Gln Ser Ala Val Lys Ala Glu Thr Ala
35 40 45Gly Asn Asp Asn Arg Asp Ala Ala Gln Gly Ala Gln Gly Ser Gln Asp
50 55 60Ser Gln Gly Ser Gln Asn Ala Gln Gly Ser Gln Asn Arg Glu Ser Gly65 70 75 80Asn Asn Asn Arg Asn Arg Ser Asn Asn Asn Arg Arg Gly Gly Arg Gly
85 90 95Arg Arg Gly Ser Gly Asn Ala Asn Glu Gly Ala Asn Asn Asn Ser Gly
100 105 110Asn Gln Asn Arg Gln Gly Gly Asn Arg Gly Asn Arg Gly Gly Gly Arg
115 120 125Arg Asn Val Val Lys Ser Met Gln Gly Ala Asp Leu Thr Gln Arg Leu
130 135 140Pro Glu Pro Pro Lys Ala Pro Ala Asn Gly Leu Arg Ile Tyr Ala Leu145 150 155 160Gly Gly Ile Ser Glu Ile Gly Arg Asn Met Thr Val Phe Glu Tyr Asn
165 170 175Asn Arg Leu Leu Ile Val Asp Cys Gly Val Leu Phe Pro Ser Ser Gly
180 185 190Glu Pro Gly Val Asp Leu Ile Leu Pro Asp Phe Gly Pro Ile Glu Asp
195 200 205His Leu His Arg Val Asp Ala Leu Val Val Thr His Gly His Glu Asp
210 215 220His Ile Gly Ala Ile Pro Trp Leu Leu Lys Leu Arg Asn Asp Ile Pro225 230 235 240Ile Leu Ala Ser Arg Phe Thr Leu Ala Leu Ile Ala Ala Lys Cys Lys
245 250 255Glu His Arg Gln Arg Pro Lys Leu Ile Glu Val Asn Glu Gln Ser Asn
260 265 270Glu Asp Arg Gly Pro Phe Asn Ile Arg Phe Trp Ala Val Asn His Ser
275 280 285Ile Pro Asp Cys Leu Gly Leu Ala Ile Lys Thr Pro Ala Gly Leu Val
290 295 300Ile His Thr Gly Asp Ile Lys Leu Asp Gln Thr305 310 315<210>221<211>789<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(766)<223>RXA02410<400>221tatgagactg accatccttg gaagctctgg tagcgtgccc gctccaggta accccgcatc 60cggatatctg ttaacttctc cggacgcccc tgccgtgatt atg gac atg ggc cca 115
Met Asp Met Gly Pro
1 5ggt gtc ctt gca gca gtt caa gaa att caa gat cct gct gat gcg cat 163Gly Val Leu Ala Ala Val Gln Glu Ile Gln Asp Pro Ala Asp Ala His
10 15 20gtt att ttc tcc cat ttg cac acc gat cac tgc gct gat ttt gcg tcc 211Val Ile Phe Ser His Leu His Thr Asp His Cys Ala Asp Phe Ala Ser
25 30 35ttg atg gtg tgg cgc agg ttc cac cca acg ctg gcc gcc aag agc cgc 259Leu Met Val Trp Arg Arg Phe His Pro Thr Leu Ala Ala Lys Ser Arg
40 45 50aat ctt ttg ttt gga cct gaa gat acc ccc aac agg ctt ggt cgt ttg 307Asn Leu Leu Phe Gly Pro Glu Asp Thr Pro Asn Arg Leu Gly Arg Leu
55 60 65agc tcc gat gag cct gat ggc gtt gac gat atg tca gat act ttt gct 355Ser Ser Asp Glu Pro Asp Gly Val Asp Asp Met Ser Asp Thr Phe Ala70 75 80 85ttc gac gcc tgg gaa gag cgc aag cca gag ctc att gat aat ttc acg 403Phe Asp Ala Trp Glu Glu Arg Lys Pro Glu Leu Ile Asp Asn Phe Thr
90 95 100gtc acg ccg ttc cgc gtt gtg cac ccc att gag acc tac gcg ctt cgc 451Val Thr Pro Phe Arg Val Val His Pro Ile Glu Thr Tyr Ala Leu Arg
105 110 115gta gag gag cac cgc acc ggc gcc tca att acg tat tcc ggt gac agc 499Val Glu Glu His Arg Thr Gly Ala Ser Ile Thr Tyr Ser Gly Asp Ser
120 125 130gcg tac acc gaa gcg ctt atc gac gcc gcc cgc aac gtt gac att ttc 547Ala Tyr Thr Glu Ala Leu Ile Asp Ala Ala Arg Asn Val Asp Ile Phe
135 140 145ttg tgc gag gca act tgg ggc acc tct tgc gat gac aaa gca cca gga 595Leu Cys Glu Ala Thr Trp Gly Thr Ser Cys Asp Asp Lys Ala Pro Gly150 155 160 165atg cat atg tgt ggc caa gac gcc gga aga att gcg gca gca gct ggc 643Met His Met Cys Gly Gln Asp Ala Gly Arg Ile Ala Ala Ala Ala Gly
170 175 180gta aag aaa ctg att atc act cat gtt cca cca tgg att gat gca gag 691Val Lys Lys Leu Ile Ile Thr His Val Pro Pro Trp Ile Asp Ala Glu
185 190 195gcc aca gtg gca gca gct gcg gaa cac ttt gat ggt cct atc gaa ttg 739Ala Thr Val Ala Ala Ala Ala Glu His Phe Asp Gly Pro Ile Glu Leu
200 205 210gca cga tca gga atg gtt atc gag ttt tagtccgttt gtactaataa 786Ala Arg Ser Gly Met Val Ile Glu Phe
215 220ggt 789<210>222<211>222<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>222Met Asp Met Gly Pro Gly Val Leu Ala Ala Val Gln Glu Ile Gln Asp1 5 10 15Pro Ala Asp Ala His Val Ile Phe Ser His Leu His Thr Asp His Cys
20 25 30Ala Asp Phe Ala Ser Leu Met Val Trp Arg Arg Phe His Pro Thr Leu
35 40 45Ala Ala Lys Ser Arg Asn Leu Leu Phe Gly Pro Glu Asp Thr Pro Asn
50 55 60Arg Leu Gly Arg Leu Ser Ser Asp Glu Pro Asp Gly Val Asp Asp Met65 70 75 80Ser Asp Thr Phe Ala Phe Asp Ala Trp Glu Glu Arg Lys Pro Glu Leu
85 90 95Ile Asp Asn Phe Thr Val Thr Pro Phe Arg Val Val His Pro Ile Glu
100 105 110Thr Tyr Ala Leu Arg Val Glu Glu His Arg Thr Gly Ala Ser Ile Thr
115 120 125Tyr Ser Gly Asp Ser Ala Tyr Thr Glu Ala Leu Ile Asp Ala Ala Arg
130 135 140Asn Val Asp Ile Phe Leu Cys Glu Ala Thr Trp Gly Thr Ser Cys Asp145 150 155 160Asp Lys Ala Pro Gly Met His Met Cys Gly Gln Asp Ala Gly Arg Ile
165 170 175Ala Ala Ala Ala Gly Val Lys Lys Leu Ile Ile Thr His Val Pro Pro
180 185 190Trp Ile Asp Ala Glu Ala Thr Val Ala Ala Ala Ala Glu His Phe Asp
195 200 205Gly Pro Ile Glu Leu Ala Arg Ser Gly Met Val Ile Glu Phe
210 215 220<210>223<211>455<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(432)<223>RXA00961<400>223cta gag aac tgg cgt atc ggc cgc atg ttg ctg ctt ggc gac gcc gcc 48Leu Glu Asn Trp Arg Ile Gly Arg Met Leu Leu Leu Gly Asp Ala Ala1 5 10 15cac gca ccc ctc cag tac ctc gcc tca ggc gcg gtc atg gcc atg gaa 96His Ala Pro Leu Gln Tyr Leu Ala Ser Gly Ala Val Met Ala Met Glu
20 25 30gac gcc gag gct gtc gcc ctc ttc gct gcc gac gct gcg cgt gct ggc 144Asp Ala Glu Ala Val Ala Leu Phe Ala Ala Asp Ala Ala Arg Ala Gly
35 40 45aac ctc gat tgg gaa gag gta ctc gca gag gtg gaa gct gaa cgc cga 192Asn Leu Asp Trp Glu Glu Val Leu Ala Glu Val Glu Ala Glu Arg Arg
50 55 60cca cgc tgc agc cgc atc caa acc gta ggc cgt ttc tgg gga gag ctc 240Pro Arg Cys Ser Arg Ile Gln Thr Val Gly Arg Phe Trp Gly Glu Leu65 70 75 80tgg cat gtg gaa ggc acc gca cgt ctc atc cgc aac gaa gtt ttc cgc 288Trp His Val Glu Gly Thr Ala Arg Leu Ile Arg Asn Glu Val Phe Arg
85 90 95caa gca gac cgc aat ggc tgg ttc atc tat gca gac tgg ctg tgg ggt 336Gln Ala Asp Arg Asn Gly Trp Phe Ile Tyr Ala Asp Trp Leu Trp Gly
100 105 110tac gat gca tcc aag cgt gcc cac atc gcc aac cct gag ctc gga gaa 384Tyr Asp Ala Ser Lys Arg Ala His Ile Ala Asn Pro Glu Leu Gly Glu
115 120 125atg cca caa gca ctg aag gaa tgg cgc tac gcc ctc ctc gaa cag aaa 432Met Pro Gln Ala Leu Lys Glu Trp Arg Tyr Ala Leu Leu Glu Gln Lys
130 135 140tagcagcctc acctgttaag gga 455<210>224<211>144<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>224Leu Glu Asn Trp Arg Ile Gly Arg Met Leu Leu Leu Gly Asp Ala Ala1 5 10 15His Ala Pro Leu Gln Tyr Leu Ala Ser Gly Ala Val Met Ala Met Glu
20 25 30Asp Ala Glu Ala Val Ala Leu Phe Ala Ala Asp Ala Ala Arg Ala Gly
35 40 45Asn Leu Asp Trp Glu Glu Val Leu Ala Glu Val Glu Ala Glu Arg Arg
50 55 60Pro Arg Cys Ser Arg Ile Gln Thr Val Gly Arg Phe Trp Gly Glu Leu65 70 75 80Trp His Val Glu Gly Thr Ala Arg Leu Ile Arg Asn Glu Val Phe Arg
85 90 95Gln Ala Asp Arg Asn Gly Trp Phe Ile Tyr Ala Asp Trp Leu Trp Gly
100 105 110Tyr Asp Ala Ser Lys Arg Ala His Ile Ala Asn Pro Glu Leu Gly Glu
115 120 125Met Pro Gln Ala Leu Lys Glu Trp Arg Tyr Ala Leu Leu Glu Gln Lys
130 135 140<210>225<211>1116<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1093)<223>RXA00111<400>225ccgagaagct ggagaaggcc aacaagcgtg gcctctacac ctccgcgtcc ttccacagcc 60ccggcgccat cactggcgac cactaaaaaa ggagacttcg atg gcc ttt ttt agc 115
Met Ala Phe Phe Ser
1 5ttt tcg acg tct ccc ctc acc cgc ctc atc ccc ggc agc cgc tcc aaa 163Phe Ser Thr Ser Pro Leu Thr Arg Leu Ile Pro Gly Ser Arg Ser Lys
10 15 20gcc aca ggc gcc aaa cgg cgc ctg agc agc aca atc gcg tcg att gaa 211Ala Thr Gly Ala Lys Arg Arg Leu Ser Ser Thr Ile Ala Ser Ile Glu
25 30 35cgc tcc ccc ggc atc att gcc cta gac gga ccg ttc acc cac gat cac 259Arg Ser Pro Gly Ile Ile Ala Leu Asp Gly Pro Phe Thr His Asp His
40 45 50gtc tcc gta cgt ggc att cgc ctc cat tta gca gag gca ggc tcc ccc 307Val Ser Val Arg Gly Ile Arg Leu His Leu Ala Glu Ala Gly Ser Pro
55 60 65acc aaa ccc ctg gtt ctt ctg atc cac ggg gct ttc ggc ggt tgg tac 355Thr Lys Pro Leu Val Leu Leu Ile His Gly Ala Phe Gly Gly Trp Tyr70 75 80 85gac tac cgc gaa gtc atc ggc cca ctc gca gat gcc ggc ttc cac gtc 403Asp Tyr Arg Glu Val Ile Gly Pro Leu Ala Asp Ala Gly Phe His Val
90 95 100gcc gcc atc gat cta cgc ggc tac ggc atg tcc gac aaa ccc cca aca 451Ala Ala Ile Asp Leu Arg Gly Tyr Gly Met Ser Asp Lys Pro Pro Thr
105 110 115ggc tac gac ctc cgc cac gca gcc gga gaa ctc agc agc gtt atc gca 499Gly Tyr Asp Leu Arg His Ala Ala Gly Glu Leu Ser Ser Val Ile Ala
120 125 130gct ctc ggc cac gat gac gca ctt ctt gtc ggc tcc gac acc ggc gcc 547Ala Leu Gly His Asp Asp Ala Leu Leu Val Gly Ser Asp Thr Gly Ala
135 140 145agc atc gcc tgg gct atc gct tcc atg tac ccc gaa cgg gtc cgc ggc 595Ser Ile Ala Trp Ala Ile Ala Ser Met Tyr Pro Glu Arg Val Arg Gly150 155 160 165cta att tcc ctc ggc gcg atc cac ccc ctt gac atg cga cgc gcc atc 643Leu Ile Ser Leu Gly Ala Ile His Pro Leu Asp Met Arg Arg Ala Ile
170 175 180cga cga aaa ccc cac cta cac gtc tct gac ctc agc cga ctt gct cct 691Arg Arg Lys Pro His Leu His Val Ser Asp Leu Ser Arg Leu Ala Pro
185 190 195ttt cgg ttg ccc tca ttc ctg cat aac ctc ttc cac ttc gga atc acc 739Phe Arg Leu Pro Ser Phe Leu His Asn Leu Phe His Phe Gly Ile Thr
200 205 210agc gaa gct cga cgt gag atc gtc aac aac acg tcc tcg tcc tac cag 787Ser Glu Ala Arg Arg Glu Ile Val Asn Asn Thr Ser Ser Ser Tyr Gln
215 220 225cgc agc aac gca ttc aca gag aca gtg ctc ctc cgc aaa aaa gca cta 835Arg Ser Asn Ala Phe Thr Glu Thr Val Leu Leu Arg Lys Lys Ala Leu230 235 240 245tcg atc gac cac acc atc acc ccg atc atc cgc acc aac cgc tac ctc 883Ser Ile Asp His Thr Ile Thr Pro Ile Ile Arg Thr Asn Arg Tyr Leu
250 255 260gtt ggg tcg atc ccc agc aaa aca gtc tcc gca ccg gtg tgg ctg ctc 931Val Gly Ser Ile Pro Ser Lys Thr Val Ser Ala Pro Val Trp Leu Leu
265 270 275aga acc aac act cga cgc tgg gaa cat cta gcc aat act gcg cgc act 979Arg Thr Asn Thr Arg Arg Trp Glu His Leu Ala Asn Thr Ala Arg Thr
280 285 290cga acg aca ggg cca ttc acc acc atc gcg atc ccc ggc ggc tac gaa 1027Arg Thr Thr Gly Pro Phe Thr Thr Ile Ala Ile Pro Gly Gly Tyr Glu
295 300 305ctc ccc tac ctc gag aac cct tcc gaa ttt gca gca acc atc gca gag 1075Leu Pro Tyr Leu Glu Asn Pro Ser Glu Phe Ala Ala Thr Ile Ala Glu310 315 320 325ttc gcg cgc acc acg ttt taagcactgt ggctgaggcg ctg 1116Phe Ala Arg Thr Thr Phe
330<210>226<211>331<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>226Met Ala Phe Phe Ser Phe Ser Thr Ser Pro Leu Thr Arg Leu Ile Pro1 5 10 15Gly Ser Arg Ser Lys Ala Thr Gly Ala Lys Arg Arg Leu Ser Ser Thr
20 25 30Ile Ala Ser Ile Glu Arg Ser Pro Gly Ile Ile Ala Leu Asp Gly Pro
35 40 45Phe Thr His Asp His Val Ser Val Arg Gly Ile Arg Leu His Leu Ala
50 55 60Glu Ala Gly Ser Pro Thr Lys Pro Leu Val Leu Leu Ile His Gly Ala65 70 75 80Phe Gly Gly Trp Tyr Asp Tyr Arg Glu Val Ile Gly Pro Leu Ala Asp
85 90 95Ala Gly Phe His Val Ala Ala Ile Asp Leu Arg Gly Tyr Gly Met Ser
100 105 110Asp Lys Pro Pro Thr Gly Tyr Asp Leu Arg His Ala Ala Gly Glu Leu
115 120 125Ser Ser Val Ile Ala Ala Leu Gly His Asp Asp Ala Leu Leu Val Gly
130 135 140Ser Asp Thr Gly Ala Ser Ile Ala Trp Ala Ile Ala Ser Met Tyr Pro145 150 155 160Glu Arg Val Arg Gly Leu Ile Ser Leu Gly Ala Ile His Pro Leu Asp
165 170 175Met Arg Arg Ala Ile Arg Arg Lys Pro His Leu His Val Ser Asp Leu
180 185 190Ser Arg Leu Ala Pro Phe Arg Leu Pro Ser Phe Leu His Asn Leu Phe
195 200 205His Phe Gly Ile Thr Ser Glu Ala Arg Arg Glu Ile Val Asn Asn Thr
210 215 220Ser Ser Ser Tyr Gln Arg Ser Asn Ala Phe Thr Glu Thr Val Leu Leu225 230 235 240Arg Lys Lys Ala Leu Ser Ile Asp His Thr Ile Thr Pro Ile Ile Arg
245 250 255Thr Asn Arg Tyr Leu Val Gly Ser Ile Pro Ser Lys Thr Val Ser Ala
260 265 270Pro Val Trp Leu Leu Arg Thr Asn Thr Arg Arg Trp Glu His Leu Ala
275 280 285Asn Thr Ala Arg Thr Arg Thr Thr Gly Pro Phe Thr Thr Ile Ala Ile
290 295 300Pro Gly Gly Tyr Glu Leu Pro Tyr Leu Glu Asn Pro Ser Glu Phe Ala305 310 315 320Ala Thr Ile Ala Glu Phe Ala Arg Thr Thr Phe
325 330<210>227<211>1020<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(997)<223>RXA01932<400>227tttctaacct gcatccaagc ctaggtggaa ttgagatgac gcgtcgtaga gatcgaaaac 60tcaaccaaat ttcttgccta aggcttctag gattgtcgtt atg ctc ctt cac cca 115
Met Leu Leu His Pro
1 5gat gcg cag ttt tat atc gat acc ttg ccc act ctc agc gcg gag gag 163Asp Ala Gln Phe Tyr Ile Asp Thr Leu Pro Thr Leu Ser Ala Glu Glu
10 15 20cag gtg agt ttt ggt aaa gac gct cct gtt tca gag gct gat gca acc 211Gln Val Ser Phe Gly Lys Asp Ala Pro Val Ser Glu Ala Asp Ala Thr
25 30 35cat gtg gcg aca gat caa gat att gct ggg gtg ccg gtg agg gtt tat 259His Val Ala Thr Asp Gln Asp Ile Ala Gly Val Pro Val Arg Val Tyr
40 45 50acg cct tta tct ggg gct ggg gat ttg ccg tgt ttg gtg tac ttc cac 307Thr Pro Leu Ser Gly Ala Gly Asp Leu Pro Cys Leu Val Tyr Phe His
55 60 65ggc ggt ggc tgg tcc ggc ggc acc ctc aac atg atc gat gcc acg gtt 355Gly Gly Gly Trp Ser Gly Gly Thr Leu Asn Met Ile Asp Ala Thr Val70 75 80 85cac tct cta gtg gtt ggc ctg ccg atc atc gcc atc agc gtg gac tac 403His Ser Leu Val Val Gly Leu Pro Ile Ile Ala Ile Ser Val Asp Tyr
90 95 100cga ctt gca ccc gca cac cca ttt cca gcg gct atc gac gac gcg ttt 451Arg Leu Ala Pro Ala His Pro Phe Pro Ala Ala Ile Asp Asp Ala Phe
105 110 115gca gtg gtc agt gcc gta ttg gat ggg gtg tct ggg ctg agt att gat 499Ala Val Val Ser Ala Val Leu Asp Gly Val Ser Gly Leu Ser Ile Asp
120 125 130act tcc cga gtg gca att ggc ggt gac agt gcc ggt gga aat att gcc 547Thr Ser Arg Val Ala Ile Gly Gly Asp Ser Ala Gly Gly Asn Ile Ala
135 140 145gcg gtt act gca caa cag ctg cgt gaa cgg gct gtg ggt tct act cct 595Ala Val Thr Ala Gln Gln Leu Arg Glu Arg Ala Val Gly Ser Thr Pro150 155 160 165gta ttg gct cac cag gtg ctt att ttt ccg gta act gat gtt tcc act 643Val Leu Ala His Gln Val Leu Ile Phe Pro Val Thr Asp Val Ser Thr
170 175 180aca tct acg ccg agc tat ctc aca ttt ggc aaa gat tgc tac ctg aca 691Thr Ser Thr Pro Ser Tyr Leu Thr Phe Gly Lys Asp Cys Tyr Leu Thr
185 190 195aag gac gcg atg gaa cgc tac atc gaa caa tat gcc gat ggg cac gac 739Lys Asp Ala Met Glu Arg Tyr Ile Glu Gln Tyr Ala Asp Gly His Asp
200 205 210cgc acc gac cct cga ctc tca ccg cta ctg gca tct gat ttg agc gac 787Arg Thr Asp Pro Arg Leu Ser Pro Leu Leu Ala Ser Asp Leu Ser Asp
215 220 225ctc cca ccc acc acc att gtg tac ggc gaa tgc gac gtg tta gcc cat 835Leu Pro Pro Thr Thr Ile Val Tyr Gly Glu Cys Asp Val Leu Ala His230 235 240 245gaa gtg cga gcc tat gga caa gct cta cta gag gct gga aat tcc gtg 883Glu Val Arg Ala Tyr Gly Gln Ala Leu Leu Glu Ala Gly Asn Ser Val
250 255 260acg atg act gaa ttc aaa gga cag atc cac gcc ttt att aac cta ggg 931Thr Met Thr Glu Phe Lys Gly Gln Ile His Ala Phe Ile Asn Leu Gly
265 270 275gga atc agt tcc gat gcg cgg gct gct cga cga ctc atc cgc gcc gaa 979Gly Ile Ser Ser Asp Ala Arg Ala Ala Arg Arg Leu Ile Arg Ala Glu
280 285 290ttg gaa gca gca ctt tgt taaaggttga gatttaacat tcg 1020Leu Glu Ala Ala Leu Cys
295<210>228<211>299<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>228Met Leu Leu His Pro Asp Ala Gln Phe Tyr Ile Asp Thr Leu Pro Thr1 5 10 15Leu Ser Ala Glu Glu Gln Val Ser Phe Gly Lys Asp Ala Pro Val Ser
20 25 30Glu Ala Asp Ala Thr His Val Ala Thr Asp Gln Asp Ile Ala Gly Val
35 40 45Pro Val Arg Val Tyr Thr Pro Leu Ser Gly Ala Gly Asp Leu Pro Cys
50 55 60Leu Val Tyr Phe His Gly Gly Gly Trp Ser Gly Gly Thr Leu Asn Met65 70 75 80Ile Asp Ala Thr Val His Ser Leu Val Val Gly Leu Pro Ile Ile Ala
85 90 95Ile Ser Val Asp Tyr Arg Leu Ala Pro Ala His Pro Phe Pro Ala Ala
100 105 110Ile Asp Asp Ala Phe Ala Val Val Ser Ala Val Leu Asp Gly Val Ser
115 120 125Gly Leu Ser Ile Asp Thr Ser Arg Val Ala Ile Gly Gly Asp Ser Ala
130 135 140Gly Gly Asn Ile Ala Ala Val Thr Ala Gln Gln Leu Arg Glu Arg Ala145 150 155 160Val Gly Ser Thr Pro Val Leu Ala His Gln Val Leu Ile Phe Pro Val
165 170 175Thr Asp Val Ser Thr Thr Ser Thr Pro Ser Tyr Leu Thr Phe Gly Lys
180 185 190Asp Cys Tyr Leu Thr Lys Asp Ala Met Glu Arg Tyr Ile Glu Gln Tyr
195 200 205Ala Asp Gly His Asp Arg Thr Asp Pro Arg Leu Ser Pro Leu Leu Ala
210 215 220Ser Asp Leu Ser Asp Leu Pro Pro Thr Thr Ile Val Tyr Gly Glu Cys225 230 235 240Asp Val Leu Ala His Glu Val Arg Ala Tyr Gly Gln Ala Leu Leu Glu
245 250 255Ala Gly Asn Ser Val Thr Met Thr Glu Phe Lys Gly Gln Ile His Ala
260 265 270Phe Ile Asn Leu Gly Gly Ile Ser Ser Asp Ala Arg Ala Ala Arg Arg
275 280 285Leu Ile Arg Ala Glu Leu Glu Ala Ala Leu Cys
290 295<210>229<211>1131<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1108)<223>RXA02574<400>229tgtgctcctt gcgggctgcg cagaagagcc ggaacagcaa aaagcaataa gccgcttatc 60gacgtccccc tccacccctc ccgcaccgac cgcggaggat ttg gcg cgc gcg caa 115
Leu Ala Arg Ala Gln
1 5atc cct gaa cag caa cgc gac caa gtc gcg tcg ctg atg atg gtt gga 163Ile Pro Glu Gln Gln Arg Asp Gln Val Ala Ser Leu Met Met Val Gly
10 15 20gtt gcg aat tat gat cag gca ttg gat gcg ctc aat cag ggg gtg ggt 211Val Ala Asn Tyr Asp Gln Ala Leu Asp Ala Leu Asn Gln Gly Val Gly
25 30 35ggc atc ttt att ggt tcc tgg aca gat gaa aat ctg ctc acg gaa cct 259Gly Ile Phe Ile Gly Ser Trp Thr Asp Glu Asn Leu Leu Thr Glu Pro
40 45 50ggc cgt aat att gag gcg ctc cgc gaa gcc gtc ggc agg gat ttc tcc 307Gly Arg Asn Ile Glu Ala Leu Arg Glu Ala Val Gly Arg Asp Phe Ser
55 60 65gtc agc atc gac ttc gaa ggc ggc cgc gtc cag cgt gcc acc aat att 355Val Ser Ile Asp Phe Glu Gly Gly Arg Val Gln Arg Ala Thr Asn Ile70 75 80 85ctt ggt gat ttc ccc tca ccg cgc gtg atg gcg caa acc atg acg ccg 403Leu Gly Asp Phe Pro Ser Pro Arg Val Met Ala Gln Thr Met Thr Pro
90 95 100gaa caa gta gaa gat ctc gca gaa atc cta ggc act ggt tta gct gca 451Glu Gln Val Glu Asp Leu Ala Glu Ile Leu Gly Thr Gly Leu Ala Ala
105 110 115cat ggt gtg aca gtt aac ttt gca cct gtt gta gat gta gat gct tgg 499His Gly Val Thr Val Asn Phe Ala Pro Val Val Asp Val Asp Ala Trp
120 125 130ggt ctc ccc gtc gtt ggc gat cgt tcc ttt tcc aac gac cca gcc gta 547Gly Leu Pro Val Val Gly Asp Arg Ser Phe Ser Asn Asp Pro Ala Val
135 140 145gca gct act tat gcc aca gct ttt gca aag ggc tta agc aaa gta gga 595Ala Ala Thr Tyr Ala Thr Ala Phe Ala Lys Gly Leu Ser Lys Val Gly150 155 160 165att acc cca gta ttc aaa cat ttc cca ggt cac ggt cgt gca agt ggc 643Ile Thr Pro Val Phe Lys His Phe Pro Gly His Gly Arg Ala Ser Gly
170 175 180gat tcg cac acc caa gat gtg gtg acc ccc gca ctt gat gag ctt aaa 691Asp Ser His Thr Gln Asp Val Val Thr Pro Ala Leu Asp Glu Leu Lys
185 190 195act tac gac ctc atc cct tat ggt caa gca ctt tct gaa act gac gga 739Thr Tyr Asp Leu Ile Pro Tyr Gly Gln Ala Leu Ser Glu Thr Asp Gly
200 205 210gcc gtc atg gtg ggc cac atg att gtt cca ggt ctt ggc acc gac gga 787Ala Val Met Val Gly His Met Ile Val Pro Gly Leu Gly Thr Asp Gly
215 220 225gtt cca tcc tct atc gac ccc gcc acc tat caa ctg ctc cgc agt ggc 835Val Pro Ser Ser Ile Asp Pro Ala Thr Tyr Gln Leu Leu Arg Ser Gly230 235 240 245gat tac cca ggt ggc gtg cct ttc gat ggc gtg atc tac acc gac gat 883Asp Tyr Pro Gly Gly Val Pro Phe Asp Gly Val Ile Tyr Thr Asp Asp
250 255 260ctc tct gga atg agt gcc att tcc gcc acc cat tca ccc gca gaa gca 931Leu Ser Gly Met Ser Ala Ile Ser Ala Thr His Ser Pro Ala Glu Ala
265 270 275gtg ctt gcc tcc ctc aaa gca ggc gca gac caa gca cta tgg atc gac 979Val Leu Ala Ser Leu Lys Ala Gly Ala Asp Gln Ala Leu Trp Ile Asp
280 285 290tat ggg tcg ttg ggc tcc gcg att gat cgc gtt gat gct gcc gtt agc 1027Tyr Gly Ser Leu Gly Ser Ala Ile Asp Arg Val Asp Ala Ala Val Ser
295 300 305agc ggt gaa tac cct caa gaa caa atg ctg gca tct gcg tta aga gtc 1075Ser Gly Glu Tyr Pro Gln Glu Gln Met Leu Ala Ser Ala Leu Arg Val310 315 320 325caa ttg ctc tac atc aca cgt ctc gaa caa aag tgaagttacc agtccgtaac 1128Gln Leu Leu Tyr Ile Thr Arg Leu Glu Gln Lys
330 335ccc1131<210>230<211>336<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>230Leu Ala Arg Ala Gln Ile Pro Glu Gln Gln Arg Asp Gln Val Ala Ser1 5 10 15Leu Met Met Val Gly Val Ala Asn Tyr Asp Gln Ala Leu Asp Ala Leu
20 25 30Asn Gln Gly Val Gly Gly Ile Phe Ile Gly Ser Trp Thr Asp Glu Asn
35 40 45Leu Leu Thr Glu Pro Gly Arg Asn Ile Glu Ala Leu Arg Glu Ala Val
50 55 60Gly Arg Asp Phe Ser Val Ser Ile Asp Phe Glu Gly Gly Arg Val Gln65 70 75 80Arg Ala Thr Asn Ile Leu Gly Asp Phe Pro Ser Pro Arg Val Met Ala
85 90 95Gln Thr Met Thr Pro Glu Gln Val Glu Asp Leu Ala Glu Ile Leu Gly
100 105 110Thr Gly Leu Ala Ala His Gly Val Thr Val Asn Phe Ala Pro Val Val
115 120 125Asp Val Asp Ala Trp Gly Leu Pro Val Val Gly Asp Arg Ser Phe Ser
130 135 140Asn Asp Pro Ala Val Ala Ala Thr Tyr Ala Thr Ala Phe Ala Lys Gly145 150 155 160Leu Ser Lys Val Gly Ile Thr Pro Val Phe Lys His Phe Pro Gly His
165 170 175Gly Arg Ala Ser Gly Asp Ser His Thr Gln Asp Val Val Thr Pro Ala
180 185 190Leu Asp Glu Leu Lys Thr Tyr Asp Leu Ile Pro Tyr Gly Gln Ala Leu
195 200 205Ser Glu Thr Asp Gly Ala Val Met Val Gly His Met Ile Val Pro Gly
210 215 220Leu Gly Thr Asp Gly Val Pro Ser Ser Ile Asp Pro Ala Thr Tyr Gln225 230 235 240Leu Leu Arg Ser Gly Asp Tyr Pro Gly Gly Val Pro Phe Asp Gly Val
245 250 255Ile Tyr Thr Asp Asp Leu Ser Gly Met Ser Ala Ile Ser Ala Thr His
260 265 270Ser Pro Ala Glu Ala Val Leu Ala Ser Leu Lys Ala Gly Ala Asp Gln
275 280 285Ala Leu Trp Ile Asp Tyr Gly Ser Leu Gly Ser Ala Ile Asp Arg Val
290 295 300Asp Ala Ala Val Ser Ser Gly Glu Tyr Pro Gln Glu Gln Met Leu Ala305 310 315 320Ser Ala Leu Arg Val Gln Leu Leu Tyr Ile Thr Arg Leu Glu Gln Lys
325 330 335<210>231<211>1599<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1576)<223>RXN00983<400>231gtgagaaaac agtggctcaa atatcgacat cttctactca cagttcaacc tgtcgtggct 60ggaggccggc tgcattggtg tcgacgccga tgaaacgtcc gtg act gca ggt gaa 115
Val Thr Ala Gly Glu
1 5acc acc act atg aat gtc acg ttg acc aat cct ttc gac aac gca att 163Thr Thr Thr Met Asn Val Thr Leu Thr Asn Pro Phe Asp Asn Ala Ile
10 15 20ttt gac cga gca gtc tcc ctt gaa cgt ccc gaa gga tgg caa gct gag 211Phe Asp Arg Ala Val Ser Leu Glu Arg Pro Glu Gly Trp Gln Ala Glu
25 30 35gat gtt cgt gtg tcg atc cca tct gga gaa tct gtc aca atc cca gtc 259Asp Val Arg Val Ser Ile Pro Ser Gly Glu Ser Val Thr Ile Pro Val
40 45 50cag gtc aca gca ccg ctg gta gcc gac aac ggt gaa ctt cca gtg gag 307Gln Val Thr Ala Pro Leu Val Ala Asp Asn Gly Glu Leu Pro Val Glu
55 60 65gtg tcc att ctt gat gga gca gac cgc tac acg ggt cgt ctc aat ctc 355Val Ser Ile Leu Asp Gly Ala Asp Arg Tyr Thr Gly Arg Leu Asn Leu70 75 80 85act gtt cag ggt ggg caa gaa cct gca cca act tca gtg aag gtg agc 403Thr Val Gln Gly Gly Gln Glu Pro Ala Pro Thr Ser Val Lys Val Ser
90 95 100att cca aat ctc aag gac act tat gta gca ggg gag aag atc agc att 451Ile Pro Asn Leu Lys Asp Thr Tyr Val Ala Gly Glu Lys Ile Ser Ile
105 110 115aac ttt gcg gtc aac aac ccg ttt gac gtt acg gtt aat tcg gtg cca 499Asn Phe Ala Val Asn Asn Pro Phe Asp Val Thr Val Asn Ser Val Pro
120 125 130agc ctg ggg gaa ggc gag aac tgg atg cct gca aac cta cgc gga ttt 547Ser Leu Gly Glu Gly Glu Asn Trp Met Pro Ala Asn Leu Arg Gly Phe
135 140 145gat cca gag cag ggt act ccc aac tgt cgt tac aag aat tta ggc gcg 595Asp Pro Glu Gln Gly Thr Pro Asn Cys Arg Tyr Lys Asn Leu Gly Ala150 155 160 165aat aag agc tat gac tgc acc aca act acc tat gaa gtc agc gat ttg 643Asn Lys Ser Tyr Asp Cys Thr Thr Thr Thr Tyr Glu Val Ser Asp Leu
170 175 180gat gta gaa cgc gga tac gtg gat att cca acg gta tgg acg ttt act 691Asp Val Glu Arg Gly Tyr Val Asp Ile Pro Thr Val Trp Thr Phe Thr
185 190 195aac tcc gca ggc gaa acg gta tgg tcc aaa aac gtt gat gtg cct cga 739Asn Ser Ala Gly Glu Thr Val Trp Ser Lys Asn Val Asp Val Pro Arg
200 205 210gtt gaa ctc aat gga aca cag gat gct gtc act gat gca atc gta acg 787Val Glu Leu Asn Gly Thr Gln Asp Ala Val Thr Asp Ala Ile Val Thr
215 220 225gtt gat ccc atc aac cca gtt cat tcc aac ggc cag agc caa act gtt 835Val Asp Pro Ile Asn Pro Val His Ser Asn Gly Gln Ser Gln Thr Val230 235 240 245gag gtc cag gct aat gtc acc tca gag gga gat ctg cca gct gga tct 883Glu Val Gln Ala Asn Val Thr Ser Glu Gly Asp Leu Pro Ala Gly Ser
250 255 260aag gtg gcc ttt tat cta gat tca tcg ccc att gat acc gca gct gtt 931Lys Val Ala Phe Tyr Leu Asp Ser Ser Pro Ile Asp Thr Ala Ala Val
265 270 275gat gcg gaa ggg cat gcc agc atc tcg att gat gtg gac aac atc gca 979Asp Ala Glu Gly His Ala Ser Ile Ser Ile Asp Val Asp Asn Ile Ala
280 285 290agc gag cag cct gaa cgc aca ttt gag gtt cgc gcc cga ctc gtc gtt 1027Ser Glu Gln Pro Glu Arg Thr Phe Glu Val Arg Ala Arg Leu Val Val
295 300 305cca gaa gat gca cca cga tca atc gcg cgt gat gcc ttg gca cgt ttt 1075Pro Glu Asp Ala Pro Arg Ser Ile Ala Arg Asp Ala Leu Ala Arg Phe310 315 320 325aca gtc ctg tct gaa caa gtg cag cag aac tcc ttg gtg atc atg aat 1123Thr Val Leu Ser Glu Gln Val Gln Gln Asn Ser Leu Val Ile Met Asn
330 335 340cat cca gat gtg ttt tct gat gga caa aca aag act att gtc atc gca 1171His Pro Asp Val Phe Ser Asp Gly Gln Thr Lys Thr Ile Val Ile Ala
345 350 355gcg aag gcg aca gca cac gat gga tcg ccg gtg gct atc ggt act ctc 1219Ala Lys Ala Thr Ala His Asp Gly Ser Pro Val Ala Ile Gly Thr Leu
360 365 370att gca ttt cgc gtc aac ggt att gag cgg gac gtg gtt cca act aac 1267Ile Ala Phe Arg Val Asn Gly Ile Glu Arg Asp Val Val Pro Thr Asn
375 380 385gcg caa gga aca gca aag ctt cag cta gac ctc aag cca gta aat act 1315Ala Gln Gly Thr Ala Lys Leu Gln Leu Asp Leu Lys Pro Val Asn Thr390 395 400 405gaa gac gag gaa tat gaa gta aca gtt gaa gcc gag ctg gat gaa ttg 1363Glu Asp Glu Glu Tyr Glu Val Thr Val Glu Ala Glu Leu Asp Glu Leu
410 415 420act gct cag acc acg ttc aaa gta ctt gct ggt gag gaa gag gaa ccc 1411Thr Ala Gln Thr Thr Phe Lys Val Leu Ala Gly Glu Glu Glu Glu Pro
425 430 435acc agc acc gaa gaa caa ccg tca gaa act gag cag cct tct gaa cct 1459Thr Ser Thr Glu Glu Gln Pro Ser Glu Thr Glu Gln Pro Ser Glu Pro
440 445 450gaa gag gaa tcg act ggt gtt gct gga agc tct aac ggt ggc agt ttt 1507Glu Glu Glu Ser Thr Gly Val Ala Gly Ser Ser Asn Gly Gly Ser Phe
455 460 465gtc gcg ctt tta gcg ctg ctg gca gcg ctt ggt ggc atc gtc ggt gca 1555Val Ala Leu Leu Ala Leu Leu Ala Ala Leu Gly Gly Ile Val Gly Ala470 475 480 485gtc ctc gga ttg ctt aag ttg taggtggctg ggggcgtcga aaa 1599Val Leu Gly Leu Leu Lys Leu
490<210>232<211>492<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>232Val Thr Ala Gly Glu Thr Thr Thr Met Asn Val Thr Leu Thr Asn Pro1 5 10 15Phe Asp Asn Ala Ile Phe Asp Arg Ala Val Ser Leu Glu Arg Pro Glu
20 25 30Gly Trp Gln Ala Glu Asp Val Arg Val Ser Ile Pro Ser Gly Glu Ser
35 40 45Val Thr Ile Pro Val Gln Val Thr Ala Pro Leu Val Ala Asp Asn Gly
50 55 60Glu Leu Pro Val Glu Val Ser Ile Leu Asp Gly Ala Asp Arg Tyr Thr65 70 75 80Gly Arg Leu Asn Leu Thr Val Gln Gly Gly Gln Glu Pro Ala Pro Thr
85 90 95Ser Val Lys Val Ser Ile Pro Asn Leu Lys Asp Thr Tyr Val Ala Gly
100 105 110Glu Lys Ile Ser Ile Asn Phe Ala Val Asn Asn Pro Phe Asp Val Thr
115 120 125Val Asn Ser Val Pro Ser Leu Gly Glu Gly Glu Asn Trp Met Pro Ala
130 135 140Asn Leu Arg Gly Phe Asp Pro Glu Gln Gly Thr Pro Asn Cys Arg Tyr145 150 155 160Lys Asn Leu Gly Ala Asn Lys Ser Tyr Asp Cys Thr Thr Thr Thr Tyr
165 170 175Glu Val Ser Asp Leu Asp Val Glu Arg Gly Tyr Val Asp Ile Pro Thr
180 185 190Val Trp Thr Phe Thr Asn Ser Ala Gly Glu Thr Val Trp Ser Lys Asn
195 200 205Val Asp Val Pro Arg Val Glu Leu Asn Gly Thr Gln Asp Ala Val Thr
210 215 220Asp Ala Ile Val Thr Val Asp Pro Ile Asn Pro Val His Ser Asn Gly225 230 235 240Gln Ser Gln Thr Val Glu Val Gln Ala Asn Val Thr Ser Glu Gly Asp
245 250 255Leu Pro Ala Gly Ser Lys Val Ala Phe Tyr Leu Asp Ser Ser Pro Ile
260 265 270Asp Thr Ala Ala Val Asp Ala Glu Gly His Ala Ser Ile Ser Ile Asp
275 280 285Val Asp Asn Ile Ala Ser Glu Gln Pro Glu Arg Thr Phe Glu Val Arg
290 295 300Ala Arg Leu Val Val Pro Glu Asp Ala Pro Arg Ser Ile Ala Arg Asp305 310 315 320Ala Leu Ala Arg Phe Thr Val Leu Ser Glu Gln Val Gln Gln Asn Ser
325 330 335Leu Val Ile Met Asn His Pro Asp Val Phe Ser Asp Gly Gln Thr Lys
340 345 350Thr Ile Val Ile Ala Ala Lys Ala Thr Ala His Asp Gly Ser Pro Val
355 360 365Ala Ile Gly Thr Leu Ile Ala Phe Arg Val Asn Gly Ile Glu Arg Asp
370 375 380Val Val Pro Thr Asn Ala Gln Gly Thr Ala Lys Leu Gln Leu Asp Leu385 390 395 400Lys Pro Val Asn Thr Glu Asp Glu Glu Tyr Glu Val Thr Val Glu Ala
405 410 415Glu Leu Asp Glu Leu Thr Ala Gln Thr Thr Phe Lys Val Leu Ala Gly
420 425 430Glu Glu Glu Glu Pro Thr Ser Thr Glu Glu Gln Pro Ser Glu Thr Glu
435 440 445Gln Pro Ser Glu Pro Glu Glu Glu Ser Thr Gly Val Ala Gly Ser Ser
450 455 460Asn Gly Gly Ser Phe Val Ala Leu Leu Ala Leu Leu Ala Ala Leu Gly465 470 475 480Gly Ile Val Gly Ala Val Leu Gly Leu Leu Lys Leu
485 490<210>233<211>1297<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1297)<223>FRXA00983<400>233gtgagaaaac agtggctcaa atatcgacat cttctactca cagttcaacc tgtcgtggct 60ggaggccggc tgcattggtg tcgacgccga tgaaacgtcc gtg act gca ggt gaa 115
Val Thr Ala Gly Glu
1 5acc acc act atg aat gtc acg ttg acc aat cct ttc gac aac gca att 163Thr Thr Thr Met Asn Val Thr Leu Thr Asn Pro Phe Asp Asn Ala Ile
10 15 20ttt gac cga gca gtc tcc ctt gaa cgt ccc gaa gga tgg caa gct gag 211Phe Asp Arg Ala Val Ser Leu Glu Arg Pro Glu Gly Trp Gln Ala Glu
25 30 35gat gtt cgt gtg tcg atc cca tct gga gaa tct gtc aca atc cca gtc 259Asp Val Arg Val Ser Ile Pro Ser Gly Glu Ser Val Thr Ile Pro Val
40 45 50cag gtc aca gca ccg ctg gta gcc gac aac ggt gaa ctt cca gtg gag 307Gln Val Thr Ala Pro Leu Val Ala Asp Asn Gly Glu Leu Pro Val Glu
55 60 65gtg tcc att ctt gat gga gca gac cgc tac acg ggt cgt ctc aat ctc 355Val Ser Ile Leu Asp Gly Ala Asp Arg Tyr Thr Gly Arg Leu Asn Leu70 75 80 85act gtt cag ggt ggg caa gaa cct gca cca act tca gtg aag gtg agc 403Thr Val Gln Gly Gly Gln Glu Pro Ala Pro Thr Ser Val Lys Val Ser
90 95 100att cca aat ctc aag gac act tat gta gca ggg gag aag atc agc att 451Ile Pro Asn Leu Lys Asp Thr Tyr Val Ala Gly Glu Lys Ile Ser Ile
105 110 115aac ttt gcg gtc aac aac ccg ttt gac gtt acg gtt aat tcg gtg cca 499Asn Phe Ala Val Asn Asn Pro Phe Asp Val Thr Val Asn Ser Val Pro
120 125 130agc ctg ggg gaa ggc gag aac tgg atg cct gca aac cta cgc gga ttt 547Ser Leu Gly Glu Gly Glu Asn Trp Met Pro Ala Asn Leu Arg Gly Phe
135 140 145gat cca gag cag ggt act ccc aac tgt cgt tac aag aat tta ggc gcg 595Asp Pro Glu Gln Gly Thr Pro Asn Cys Arg Tyr Lys Asn Leu Gly Ala150 155 160 165aat aag agc tat gac tgc acc aca act acc tat gaa gtc agc gat ttg 643Asn Lys Ser Tyr Asp Cys Thr Thr Thr Thr Tyr Glu Val Ser Asp Leu
170 175 180gat gta gaa cgc gga tac gtg gat att cca acg gta tgg acg ttt act 691Asp Val Glu Arg Gly Tyr Val Asp Ile Pro Thr Val Trp Thr Phe Thr
185 190 195aac tcc gca ggc gaa acg gta tgg tcc aaa aac gtt gat gtg cct cga 739Asn Ser Ala Gly Glu Thr Val Trp Ser Lys Asn Val Asp Val Pro Arg
200 205 210gtt gaa ctc aat gga aca cag gat gct gtc act gat gca atc gta acg 787Val Glu Leu Asn Gly Thr Gln Asp Ala Val Thr Asp Ala Ile Val Thr
215 220 225gtt gat ccc atc aac cca gtt cat tcc aac ggc cag agc caa act gtt 835Val Asp Pro Ile Asn Pro Val His Ser Asn Gly Gln Ser Gln Thr Val230 235 240 245gag gtc cag gct aat gtc acc tca gag gga gat ctg cca gct gga tct 883Glu Val Gln Ala Asn Val Thr Ser Glu Gly Asp Leu Pro Ala Gly Ser
250 255 260aag gtg gcc ttt tat cta gat tca tcg ccc att gat acc gca gct gtt 931Lys Val Ala Phe Tyr Leu Asp Ser Ser Pro Ile Asp Thr Ala Ala Val
265 270 275gat gcg gaa ggg cat gcc agc atc tcg att gat gtg gac aac atc gca 979Asp Ala Glu Gly His Ala Ser Ile Ser Ile Asp Val Asp Asn Ile Ala
280 285 290agc gag cag cct gaa cgc aca ttt gag gtt cgc gcc cga ctc gtc gtt 1027Ser Glu Gln Pro Glu Arg Thr Phe Glu Val Arg Ala Arg Leu Val Val
295 300 305cca gaa gat gca cca cga tca atc gcg cgt gat gcc ttg gca cgt ttt 1075Pro Glu Asp Ala Pro Arg Ser Ile Ala Arg Asp Ala Leu Ala Arg Phe310 315 320 325aca gtc ctg tct gaa caa gtg cag cag aac tcc ttg gtg atc atg aat 1123Thr Val Leu Ser Glu Gln Val Gln Gln Asn Ser Leu Val Ile Met Asn
330 335 340cat cca gat gtg ttt tct gat gga caa aca aag act att gtc atc gca 1171His Pro Asp Val Phe Ser Asp Gly Gln Thr Lys Thr Ile Val Ile Ala
345 350 355gcg aag gcg aca gca cac gat gga tcg ccg gcg gct atc ggt act ctc 1219Ala Lys Ala Thr Ala His Asp Gly Ser Pro Ala Ala Ile Gly Thr Leu
360 365 370att gca ttt cgc gtc aac ggt att gag cgg gac gtg gtt cca act aac 1267Ile Ala Phe Arg Val Asn Gly Ile Glu Arg Asp Val Val Pro Thr Asn
375 380 385gcg caa gga aca gca aag ctt cag cta gac 1297Ala Gln Gly Thr Ala Lys Leu Gln Leu Asp390 395<210>234<211>399<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>234Val Thr Ala Gly Glu Thr Thr Thr Met Asn Val Thr Leu Thr Asn Pro1 5 10 15Phe Asp Asn Ala Ile Phe Asp Arg Ala Val Ser Leu Glu Arg Pro Glu
20 25 30Gly Trp Gln Ala Glu Asp Val Arg Val Ser Ile Pro Ser Gly Glu Ser
35 40 45Val Thr Ile Pro Val Gln Val Thr Ala Pro Leu Val Ala Asp Asn Gly
50 55 60Glu Leu Pro Val Glu Val Ser Ile Leu Asp Gly Ala Asp Arg Tyr Thr65 70 75 80Gly Arg Leu Asn Leu Thr Val Gln Gly Gly Gln Glu Pro Ala Pro Thr
85 90 95Ser Val Lys Val Ser Ile Pro Asn Leu Lys Asp Thr Tyr Val Ala Gly
100 105 110Glu Lys Ile Ser Ile Asn Phe Ala Val Asn Asn Pro Phe Asp Val Thr
115 120 125Val Asn Ser Val Pro Ser Leu Gly Glu Gly Glu Asn Trp Met Pro Ala
130 135 140Asn Leu Arg Gly Phe Asp Pro Glu Gln Gly Thr Pro Asn Cys Arg Tyr145 150 155 160Lys Asn Leu Gly Ala Asn Lys Ser Tyr Asp Cys Thr Thr Thr Thr Tyr
165 170 175Glu Val Ser Asp Leu Asp Val Glu Arg Gly Tyr Val Asp Ile Pro Thr
180 185 190Val Trp Thr Phe Thr Asn Ser Ala Gly Glu Thr Val Trp Ser Lys Asn
195 200 205Val Asp Val Pro Arg Val Glu Leu Asn Gly Thr Gln Asp Ala Val Thr
210 215 220Asp Ala Ile Val Thr Val Asp Pro Ile Asn Pro Val His Ser Asn Gly225 230 235 240Gln Ser Gln Thr Val Glu Val Gln Ala Asn Val Thr Ser Glu Gly Asp
245 250 255Leu Pro Ala Gly Ser Lys Val Ala Phe Tyr Leu Asp Ser Ser Pro Ile
260 265 270Asp Thr Ala Ala Val Asp Ala Glu Gly His Ala Ser Ile Ser Ile Asp
275 280 285Val Asp Asn Ile Ala Ser Glu Gln Pro Glu Arg Thr Phe Glu Val Arg
290 295 300Ala Arg Leu Val Val Pro Glu Asp Ala Pro Arg Ser Ile Ala Arg Asp305 310 315 320Ala Leu Ala Arg Phe Thr Val Leu Ser Glu Gln Val Gln Gln Asn Ser
325 330 335Leu Val Ile Met Asn His Pro Asp Val Phe Ser Asp Gly Gln Thr Lys
340 345 350Thr Ile Val Ile Ala Ala Lys Ala Thr Ala His Asp Gly Ser Pro Ala
355 360 365Ala Ile Gly Thr Leu Ile Ala Phe Arg Val Asn Gly Ile Glu Arg Asp
370 375 380Val Val Pro Thr Asn Ala Gln Gly Thr Ala Lys Leu Gln Leu Asp385 390 395<210>235<211>440<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(417)<223>RXA00984<400>235caa cgt ggt acc cca gtg ctc ctt ggg gaa act cca tgg atg aaa aca 48Gln Arg Gly Thr Pro Val Leu Leu Gly Glu Thr Pro Trp Met Lys Thr1 5 10 15aaa atc gtg gaa ctc agc gat gga acc ctg atg aac aac agt cgt tca 96Lys Ile Val Glu Leu Ser Asp Gly Thr Leu Met Asn Asn Ser Arg Ser
20 25 30tca gga gcc gat act tac cgc aag gtg tct tat tcc acc gac ggc ggc 144Ser Gly Ala Asp Thr Tyr Arg Lys Val Ser Tyr Ser Thr Asp Gly Gly
35 40 45gtc act tgg acc gag cca act ctt gat acc cag ctg ccg gat cct cgc 192
Val Thr Trp Thr Glu Pro Thr Leu Asp Thr Gln Leu Pro Asp Pro Arg
50 55 60aac aat gct tcc ctg att cga gta ttc ccg aca gca cct gag gga agt 240Asn Asn Ala Ser Leu Ile Arg Val Phe Pro Thr Ala Pro Glu Gly Ser65 70 75 80gcg cag gca aag gtt ctg ctg ttc tcc aac act gcc acc acg agt ggc 288Ala Gln Ala Lys Val Leu Leu Phe Ser Asn Thr Ala Thr Thr Ser Gly
85 90 95cgc acc aat ggc acc gtc cgc atg tcg tgt gat gat ggt cag acc tgg 336Arg Thr Asn Gly Thr Val Arg Met Ser Cys Asp Asp Gly Gln Thr Trp
100 105 110ccg gtg tct aag gtg ttt gaa cca gga gca atc caa tat acc tcg atg 384Pro Val Ser Lys Val Phe Glu Pro Gly Ala Ile Gln Tyr Thr Ser Met
115 120 125gca acg ctt ccc aac ggt gac atc ggc atg ctg tgagaaaaca gtggctcaaa 437Ala Thr Leu Pro Asn Gly Asp Ile Gly Met Leu
130 135tat 440<210>236<211>139<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>236Gln Arg Gly Thr Pro Val Leu Leu Gly Glu Thr Pro Trp Met Lys Thr1 5 10 15Lys Ile Val Glu Leu Ser Asp Gly Thr Leu Met Asn Asn Ser Arg Ser
20 25 30Ser Gly Ala Asp Thr Tyr Arg Lys Val Ser Tyr Ser Thr Asp Gly Gly
35 40 45Val Thr Trp Thr Glu Pro Thr Leu Asp Thr Gln Leu Pro Asp Pro Arg
50 55 60Asn Asn Ala Ser Leu Ile Arg Val Phe Pro Thr Ala Pro Glu Gly Ser65 70 75 80Ala Gln Ala Lys Val Leu Leu Phe Ser Asn Thr Ala Thr Thr Ser Gly
85 90 95Arg Thr Asn Gly Thr Val Arg Met Ser Cys Asp Asp Gly Gln Thr Trp
100 105 110Pro Val Ser Lys Val Phe Glu Pro Gly Ala Ile Gln Tyr Thr Ser Met
115 120 125Ala Thr Leu Pro Asn Gly Asp Ile Gly Met Leu
130 135<210>237<211>832<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(832)<223>RXN02513<400>237acagcaccgt tttgtaggat aagaaaatcc cgcacacaac ccgtcctggt gggtgaagtg 60ggggaggcat gtctatgccc ccaattagac atctgacatc atg ctt cca atc tgg 115
Met Leu Pro Ile Trp
1 5atg ggt ctt cca ttc aag aaa gca ggt gct ttg tct cgg cgt aaa gca 163Met Gly Leu Pro Phe Lys Lys Ala Gly Ala Leu Ser Arg Arg Lys Ala
10 15 20gta ttc tca gcg ctt ggt gca gcc gca ctc atg ggc gca gca cta ccc 211Val Phe Ser Ala Leu Gly Ala Ala Ala Leu Met Gly Ala Ala Leu Pro
25 30 35acc atc cca acg gcc caa gct caa aca ccc acg ggc tac gga ttc gat 259Thr Ile Pro Thr Ala Gln Ala Gln Thr Pro Thr Gly Tyr Gly Phe Asp
40 45 50gca aca gca agc atc agc gaa gaa cca gag ttt tca aca caa caa ctc 307Ala Thr Ala Ser Ile Ser Glu Glu Pro Glu Phe Ser Thr Gln Gln Leu
55 60 65gct gac ggc gga act ctc gga ttt gat tgc tac cgc atc cca tcg ctt 355Ala Asp Gly Gly Thr Leu Gly Phe Asp Cys Tyr Arg Ile Pro Ser Leu70 75 80 85ggc gtc gca ccc aac ggc aac gtc ctc gca tcg tgg gat ggt cgc cca 403Gly Val Ala Pro Asn Gly Asn Val Leu Ala Ser Trp Asp Gly Arg Pro
90 95 100aac aac tgt tca gat gct cca caa ccc aac tcc atc gtg ggc aag gta 451Asn Asn Cys Ser Asp Ala Pro Gln Pro Asn Ser Ile Val Gly Lys Val
105 110 115tcg acc gac aac gga gca acc tgg ggc gaa cag cac gac att tcc gca 499Ser Thr Asp Asn Gly Ala Thr Trp Gly Glu Gln His Asp Ile Ser Ala
120 125 130ggt atc acc gcc gaa ccc aaa act ggc tat tcc gat ccc agc atc gtt 547Gly Ile Thr Ala Glu Pro Lys Thr Gly Tyr Ser Asp Pro Ser Ile Val
135 140 145gtg gac tgg gag agg ggc gat gtc ttt aac ttc cac gtg aag tca ttc 595Val Asp Trp Glu Arg Gly Asp Val Phe Asn Phe His Val Lys Ser Phe150 155 160 165gat gca gga tac ttc acc tcc caa cca ggc acg gac ccg gat gat cgc 643Asp Ala Gly Tyr Phe Thr Ser Gln Pro Gly Thr Asp Pro Asp Asp Arg
170 175 180aac gtt gcc cat gtt gcc tac gcc aaa tca tca gat aac ggc tca acc 691Asn Val Ala His Val Ala Tyr Ala Lys Ser Ser Asp Asn Gly Ser Thr
185 190 195tgg gtt gca gac acc gtc att act gat caa gtg gtt gct cat gac acc 739Trp Val Ala Asp Thr Val Ile Thr Asp Gln Val Val Ala His Asp Thr
200 205 210tgg gac agc cga ttt gcc aca tcc gga aac ggc atc caa ctg caa tac 787Trp Asp Ser Arg Phe Ala Thr Ser Gly Asn Gly Ile Gln Leu Gln Tyr
215 220 225ggc gcg tac aag gga cga ttg gtc cag cca tcg gta act cgc atg 832Gly Ala Tyr Lys Gly Arg Leu Val Gln Pro Ser Val Thr Arg Met230 235 240<210>238<211>244<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>238Met Leu Pro Ile Trp Met Gly Leu Pro Phe Lys Lys Ala Gly Ala Leu1 5 10 15Ser Arg Arg Lys Ala Val Phe Ser Ala Leu Gly Ala Ala Ala Leu Met
20 25 30Gly Ala Ala Leu Pro Thr Ile Pro Thr Ala Gln Ala Gln Thr Pro Thr
35 40 45Gly Tyr Gly Phe Asp Ala Thr Ala Ser Ile Ser Glu Glu Pro Glu Phe
50 55 60Ser Thr Gln Gln Leu Ala Asp Gly Gly Thr Leu Gly Phe Asp Cys Tyr65 70 75 80Arg Ile Pro Ser Leu Gly Val Ala Pro Asn Gly Asn Val Leu Ala Ser
85 90 95Trp Asp Gly Arg Pro Asn Asn Cys Ser Asp Ala Pro Gln Pro Asn Ser
100 105 110Ile Val Gly Lys Val Ser Thr Asp Asn Gly Ala Thr Trp Gly Glu Gln
115 120 125His Asp Ile Ser Ala Gly Ile Thr Ala Glu Pro Lys Thr Gly Tyr Ser
130 135 140Asp Pro Ser Ile Val Val Asp Trp Glu Arg Gly Asp Val Phe Asn Phe145 150 155 160His Val Lys Ser Phe Asp Ala Gly Tyr Phe Thr Ser Gln Pro Gly Thr
165 170 175Asp Pro Asp Asp Arg Asn Val Ala His Val Ala Tyr Ala Lys Ser Ser
180 185 190Asp Asn Gly Ser Thr Trp Val Ala Asp Thr Val Ile Thr Asp Gln Val
195 200 205Val Ala His Asp Thr Trp Asp Ser Arg Phe Ala Thr Ser Gly Asn Gly
210 215 220Ile Gln Leu Gln Tyr Gly Ala Tyr Lys Gly Arg Leu Val Gln Pro Ser225 230 235 240Val Thr Arg Met<210>239<211>824<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(93)..(824)<223>FRXA02513<400>239gtcatgtagg ataagaaaat cccgcacaca acccgtcctg gtggggtaag tgagggaggc 60atgtctatgc ccccaattag acatctgaca tc atg ctt cca atc tgg atg ggt 113
Met Leu Pro Ile Trp Met Gly
1 5ctt cca ttc aag aaa gca ggg gct ttg tct cgg cgt aaa gca gta ttc 161Leu Pro Phe Lys Lys Ala Gly Ala Leu Ser Arg Arg Lys Ala Val Phe
10 15 20tca gcg ctt ggt gca gac gca ctc atg ggc gca gca cta ccc acc atc 209Ser Ala Leu Gly Ala Asp Ala Leu Met Gly Ala Ala Leu Pro Thr Ile
25 30 35cca acg gcc caa gct caa aca ccc acg ggc tac gga ttc gat gca aca 257Pro Thr Ala Gln Ala Gln Thr Pro Thr Gly Tyr Gly Phe Asp Ala Thr40 45 50 55gca agc atc agc gaa gaa cca gag ttt tca aca caa caa ctc gct gac 305Ala Ser Ile Ser Glu Glu Pro Glu Phe Ser Thr Gln Gln Leu Ala Asp
60 65 70ggc gga act ctc gga ttt gat tgc tac cgc atc cca tcg ctt ggc gtc 353Gly Gly Thr Leu Gly Phe Asp Cys Tyr Arg Ile Pro Ser Leu Gly Val
75 80 85gca ccc aac ggc aac gtc ctc gca tcg tgg gat ggt cgc cca aac aac 401Ala Pro Asn Gly Asn Val Leu Ala Ser Trp Asp Gly Arg Pro Asn Asn
90 95 100tgt tca gat gct cca caa ccc aac tcc atc gtg ggc aag gta tcg acc 449Cys Ser Asp Ala Pro Gln Pro Asn Ser Ile Val Gly Lys Val Ser Thr
105 110 115gac aac gga gca acc tgg ggc gaa cag cac gac att tcc gca ggt atc 497Asp Asn Gly Ala Thr Trp Gly Glu Gln His Asp Ile Ser Ala Gly Ile120 125 130 135acc gcc gaa ccc aaa act ggc tat tcc gat ccc agc atc gtt gtg gac 545Thr Ala Glu Pro Lys Thr Gly Tyr Ser Asp Pro Ser Ile Val Val Asp
140 145 150tgg gag agg ggc gat gtc ttt aac ttc cac gtg aag tca ttc gat gca 593Trp Glu Arg Gly Asp Val Phe Asn Phe His Val Lys Ser Phe Asp Ala
155 160 165gga tac ttc acc tcc caa cca ggc acg gac ccg gat gat cgc aac gtt 641Gly Tyr Phe Thr Ser Gln Pro Gly Thr Asp Pro Asp Asp Arg Asn Val
170 175 180gcc cat gtt gcc tac gcc aaa tca tca gat aac ggc tca acc tgg gtt 689Ala His Val Ala Tyr Ala Lys Ser Ser Asp Asn Gly Ser Thr Trp Val
185 190 195gca gac acc gtc att act gat caa gtg gtt gct cat gac acc tgg gac 737Ala Asp Thr Val Ile Thr Asp Gln Val Val Ala His Asp Thr Trp Asp200 205 210 215agc cga ttt gcc aca tcc gga aac ggc atc caa ctg caa tac ggc gcg 785Ser Arg Phe Ala Thr Ser Gly Asn Gly Ile Gln Leu Gln Tyr Gly Ala
220 225 230tac aag gga cga ttg gtc cag cca tcg gta act cgc atg 824Tyr Lys Gly Arg Leu Val Gln Pro Ser Val Thr Arg Met
235 240<210>240<211>244<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>240Met Leu Pro Ile Trp Met Gly Leu Pro Phe Lys Lys Ala Gly Ala Leu1 5 10 15Ser Arg Arg Lys Ala Val Phe Ser Ala Leu Gly Ala Asp Ala Leu Met
20 25 30Gly Ala Ala Leu Pro Thr Ile Pro Thr Ala Gln Ala Gln Thr Pro Thr
35 40 45Gly Tyr Gly Phe Asp Ala Thr Ala Ser Ile Ser Glu Glu Pro Glu Phe
50 55 60Ser Thr Gln Gln Leu Ala Asp Gly Gly Thr Leu Gly Phe Asp Cys Tyr65 70 75 80Arg Ile Pro Ser Leu Gly Val Ala Pro Asn Gly Asn Val Leu Ala Ser
85 90 95Trp Asp Gly Arg Pro Asn Asn Cys Ser Asp Ala Pro Gln Pro Asn Ser
100 105 110Ile Val Gly Lys Val Ser Thr Asp Asn Gly Ala Thr Trp Gly Glu Gln
115 120 125His Asp Ile Ser Ala Gly Ile Thr Ala Glu Pro Lys Thr Gly Tyr Ser
130 135 140Asp Pro Ser Ile Val Val Asp Trp Glu Arg Gly Asp Val Phe Asn Phe145 150 155 160His Val Lys Ser Phe Asp Ala Gly Tyr Phe Thr Ser Gln Pro Gly Thr
165 170 175Asp Pro Asp Asp Arg Asn Val Ala His Val Ala Tyr Ala Lys Ser Ser
180 185 190Asp Asn Gly Ser Thr Trp Val Ala Asp Thr Val Ile Thr Asp Gln Val
195 200 205Val Ala His Asp Thr Trp Asp Ser Arg Phe Ala Thr Ser Gly Asn Gly
210 215 220Ile Gln Leu Gln Tyr Gly Ala Tyr Lys Gly Arg Leu Val Gln Pro Ser225 230 235 240Val Thr Arg Met<210>241<211>733<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(733)<223>RXA00903<400>241gcactcggaa tcgtcgtcct agaaaagaaa gacgcctaaa atgccctcta aaatatcgcg 60gccctattac caagtagatg tattcagctc cgagccgttc atg gga aac ccg ctt 115
Met Gly Asn Pro Leu
1 5gct gtc atc gcc gat gct gat gac tta agt gcc gaa caa atg gcc cga 163Ala Val Ile Ala Asp Ala Asp Asp Leu Ser Ala Glu Gln Met Ala Arg
10 15 20atc gct agg tgg aca aac ctc tca gaa acc aca ttt ctt tta aag cca 211Ile Ala Arg Trp Thr Asn Leu Ser Glu Thr Thr Phe Leu Leu Lys Pro
25 30 35acc caa gaa ggt gct gac tac cgg gta cgc att ttc acc cca acc ggt 259Thr Gln Glu Gly Ala Asp Tyr Arg Val Arg Ile Phe Thr Pro Thr Gly
40 45 50gag ctc ccc ttc gct gga cac cca aca ctc gga acc gcc cac gtg ttt 307Glu Leu Pro Phe Ala Gly His Pro Thr Leu Gly Thr Ala His Val Phe
55 60 65agg gaa ctg cac ggt gaa cag gga acc cag ttg gtt cag gaa tgt gtc 355Arg Glu Leu His Gly Glu Gln Gly Thr Gln Leu Val Gln Glu Cys Val70 75 80 85gcc ggt tta gtt gct gtg cgc gct att gac ggg cca gca agt gga ttg 403Ala Gly Leu Val Ala Val Arg Ala Ile Asp Gly Pro Ala Ser Gly Leu
90 95 100gct ttt cag gct cca ccc aca ctc aaa gac ggg cca ttg gat gct tcc 451Ala Phe Gln Ala Pro Pro Thr Leu Lys Asp Gly Pro Leu Asp Ala Ser
105 110 115gac cta gac gca gct tgt gag gct tta gga atc agc ccc gac ttc att 499Asp Leu Asp Ala Ala Cys Glu Ala Leu Gly Ile Ser Pro Asp Phe Ile
120 125 130cga gcc cac caa tgg gta gac aac ggc ccc ggc tgg gca gta gtg gag 547Arg Ala His Gln Trp Val Asp Asn Gly Pro Gly Trp Ala Val Val Glu
135 140 145cta ccg agc gcc caa cac gta ttg gat ctg gaa ccc gat ttc agt gca 595Leu Pro Ser Ala Gln His Val Leu Asp Leu Glu Pro Asp Phe Ser Ala150 155 160 165cat cca aca ttg aaa ctc gga gtg att ggg gcc tat ccc gaa ggg gct 643His Pro Thr Leu Lys Leu Gly Val Ile Gly Ala Tyr Pro Glu Gly Ala
170 175 180ccc cac gcc ttt gaa gta cgg gca ttc gct caa gga atc ggt gaa gac 691Pro His Ala Phe Glu Val Arg Ala Phe Ala Gln Gly Ile Gly Glu Asp
185 190 195cca gtt aca gga agc ctc aat gca ttc att gcg cag tgg cta 733Pro Val Thr Gly Ser Leu Asn Ala Phe Ile Ala Gln Trp Leu
200 205 210<210>242<211>211<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>242Met Gly Asn Pro Leu Ala Val Ile Ala Asp Ala Asp Asp Leu Ser Ala1 5 10 15Glu Gln Met Ala Arg Ile Ala Arg Trp Thr Asn Leu Ser Glu Thr Thr
20 25 30Phe Leu Leu Lys Pro Thr Gln Glu Gly Ala Asp Tyr Arg Val Arg Ile
35 40 45Phe Thr Pro Thr Gly Glu Leu Pro Phe Ala Gly His Pro Thr Leu Gly
50 55 60Thr Ala His Val Phe Arg Glu Leu His Gly Glu Gln Gly Thr Gln Leu65 70 75 80Val Gln Glu Cys Val Ala Gly Leu Val Ala Val Arg Ala Ile Asp Gly
85 90 95Pro Ala Ser Gly Leu Ala Phe Gln Ala Pro Pro Thr Leu Lys Asp Gly
100 105 110Pro Leu Asp Ala Ser Asp Leu Asp Ala Ala Cys Glu Ala Leu Gly Ile
115 120 125Ser Pro Asp Phe Ile Arg Ala His Gln Trp Val Asp Asn Gly Pro Gly
130 135 140Trp Ala Val Val Glu Leu Pro Ser Ala Gln His Val Leu Asp Leu Glu145 150 155 160Pro Asp Phe Ser Ala His Pro Thr Leu Lys Leu Gly Val Ile Gly Ala
165 170 175Tyr Pro Glu Gly Ala Pro His Ala Phe Glu Val Arg Ala Phe Ala Gln
180 185 190Gly Ile Gly Glu Asp Pro Val Thr Gly Ser Leu Asn Ala Phe Ile Ala
195 200 205Gln Trp Leu
210<210> 243<211> 1146<212> DNA<213> 谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221> CDS<222> (101)..(1123)<223> RXA01224<400> 243ttggcggcgg gaagttcagg cttgggggca aacagtgctt ggattttaga caaaaaactc 60acggaagtca tcctatggca ggcgcgccta ggatggtgcc atg agc atc ctt gac 115
Met Ser Ile Leu Asp
1 5acg ttg aaa act ccc gtg att gtc gcc ccg atg gct ggc ggc ccg tcc 163Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met Ala Gly Gly Pro Ser
10 15 20act ccc gcg ttg gtc aat gca gca gca gag gca ggt tcc ctc ggg ttc 211Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala Gly Ser Leu Gly Phe
25 30 35ttg gct ggt ggc gtc atg cct ctt gag Gag ctg aaa cag gaa ttg tca 259Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu Lys Gln Glu Leu Ser
40 45 50gag gta aaa ggc gtc ttt ggc gtc aac ctg ttt cgc ccg cag acg gat 307Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe Arg Pro Gln Thr Asp
55 60 65gcg cct aag cct tca gac att gat gag ctg gcg gga ttg ttg tcc tcg 355Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala Gly Leu Leu Ser Ser70 75 80 85gcg ttt cgg caa ttt ggc ctc gat gag ccg acg gtg cct acg ccg gat 403Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr Val Pro Thr Pro Asp
90 95 100ttg agc aat ggg tgg gag gct aaa ttt gag gcc gtt ctt gcc gct aag 451Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala Val Leu Ala Ala Lys
105 110 115ccc gcc gtt ttt tcc tgc acc ttt ggt att ttt agc gct gaa gaa ttc 499Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe Ser Ala Glu Glu Phe
120 125 130gcc cgg atc aaa gcc acc gga att gag gcg tgg gtg acg gtg acc aat 547Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp Val Thr Val Thr Asn
135 140 145ccg gag gac gcg ctg gct gcg cag aaa gct ggc gcc aac gcg ctt gtc 595Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly Ala Asn Ala Leu Val150 155 160 165gtg caa ggc ccc gag gcg ggt ggg cac cgc tct acc tgg tcc att gaa 643Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser Thr Trp Ser Ile Glu
170 175 180gtg gag ccg gac gag cgc gac ctg aaa acc ctc ctc gca gct gtc aaa 691Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu Leu Ala Ala Val Lys
185 190 195caa gcg ggc gtt tac ctc ccg ctc atc gca gcc ggc ggc ctt tca acc 739Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala Gly Gly Leu Ser Thr
200 205 210tcc gca gac gtg gca gca att tta gaa gcc ggc gcc agc gct gcc tcc 787Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly Ala Ser Ala Ala Ser
215 220 225tgt ggt tcc gcc ttt ttg ctt agc gac gaa gcc ggc acc agc tca ctt 835Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala Gly Thr Ser Ser Leu230 235 240 245aac cgc gag atc ttg gac gcc gcc cca gca ctt ggt ttg gaa tcg gtg 883Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu Gly Leu Glu Ser Val
250 255 260tca tct cgc gca ttt tcg ggc cgt tat gcc agg gga gtg gaa acc agg 931Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg Gly Val Glu Thr Arg
265 270 275ttc acc cgt tcg aac gag ggg tta ccc ccg ttg tac cca tac ctc aac 979Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu Tyr Pro Tyr Leu Asn
280 285 290cca atg atc aca tct tta cgt aag gtg gcg gga agt gca ggg aac tgg 1027Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly Ser Ala Gly Asn Trp
295 300 305gat tac gcc tac tgc ctg gta gga gtc ggc ctg gaa tcg att gcg aag 1075Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu Glu Ser Ile Ala Lys310 315 320 325ggt agt gca aag cag ata ctg gaa tca tta aca cct tcc gct ttg ggc 1123Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr Pro Ser Ala Leu Gly
330 335 340taatgttggg gggagtgctt tca 1146<210>244<211>341<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>244Met Ser Ile Leu Asp Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met1 5 10 15Ala Gly Gly Pro Ser Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala
20 25 30Gly Ser Leu Gly Phe Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu
35 40 45Lys Gln Glu Leu Ser Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe
50 55 60Arg Pro Gln Thr Asp Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala65 70 75 80Gly Leu Leu Ser Ser Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr
85 90 95Val Pro Thr Pro Asp Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala
100 105 110Val Leu Ala Ala Lys Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe
115 120 125Ser Ala Glu Glu Phe Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp
130 135 140Val Thr Val Thr Asn Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly145 150 155 160Ala Asn Ala Leu Val Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser
165 170 175Thr Trp Ser Ile Glu Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu
180 185 190Leu Ala Ala Val Lys Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala
195 200 205Gly Gly Leu Ser Thr Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly
210 215 220Ala Ser Ala Ala Ser Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala225 230 235 240Gly Thr Ser Ser Leu Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu
245 250 255Gly Leu Glu Ser Val Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg
260 265 270Gly Val Glu Thr Arg Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu
275 280 285Tyr Pro Tyr Leu Asn Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly
290 295 300Ser Ala Gly Asn Trp Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu305 310 315 320Glu Ser Ile Ala Lys Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr
325 330 335Pro Ser Ala Leu Gly
340<210>245<211>723<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(700)<223>RXA01571<400>245aaactacctg ctgagagctt tgtaatttac ggtgtggttg tggaggggtg cgtcgagaag 60cgctcgtagg cgcttttgat ttttcggtag gctaactggg gtg agt atc tca gta 115
Val Ser Ile Ser Val
1 5aaa gca cta caa aag tcc ggc cca gaa gca cct ttc gag gtc aag atc 163Lys Ala Leu Gln Lys Ser Gly Pro Glu Ala Pro Phe Glu Val Lys Ile
10 15 20att gaa cgc cgt gac cca cgc gca gat gat gtg gtt att gat atc aaa 211Ile Glu Arg Arg Asp Pro Arg Ala Asp Asp Val Val Ile Asp Ile Lys
25 30 35gct gcg ggc atc tgc cac agc gat atc cac acc atc cgc aac gaa tgg 259Ala Ala Gly Ile Cys His Ser Asp Ile His Thr Ile Arg Asn Glu Trp
40 45 50ggc gag gcg cac ttc ccg ctc acc gtc ggc cac gaa atc gca ggc gtt 307Gly Glu Ala His Phe Pro Leu Thr Val Gly His Glu Ile Ala Gly Val
55 60 65gtc tct gcg gtt gga tcc gat gta acc aaa tgg aaa gtc ggc gac cgc 355Val Ser Ala Val Gly Ser Asp Val Thr Lys Trp Lys Val Gly Asp Arg70 75 80 85gtg ggc gtc ggc tgc ctc gtt aac tcc tgc ggc gaa tgc gaa cag tgc 403Val Gly Val Gly Cys Leu Val Asn Ser Cys Gly Glu Cys Glu Gln Cys
90 95 100gtc gca gga ttt gaa aac aac tgc ctt cgc gga aac gtc gga acc tac 451Val Ala Gly Phe Glu Asn Asn Cys Leu Arg Gly Asn Val Gly Thr Tyr
105 110 115aac tct aac gac gtc gac ggc acc atc acc caa ggc ggc tac gct gaa 499Asn Ser Asn Asp Val Asp Gly Thr Ile Thr Gln Gly Gly Tyr Ala Glu
120 125 130aag gta gtg gtc aac gaa cgt ttc ctg tgc agc atc cca gag gaa ctt 547Lys Val Val Val Asn Glu Arg Phe Leu Cys Ser Ile Pro Glu Glu Leu
135 140 145aac ttc gat gtc gca gca cca ctg ctg tgc gca ggc atc acc acc tac 595Asn Phe Asp Val Ala Ala Pro Leu Leu Cys Ala Gly Ile Thr Thr Tyr150 155 160 165tcc cca atc gct cgc tgg aac gtt aaa gaa ggc gac aaa gta gca gtc 643Ser Pro Ile Ala Arg Trp Asn Val Lys Glu Gly Asp Lys Val Ala Val
170 175 180atg ggc ctc ggc ggg act cgg aca cat ggg tgt cca gat cgc tgc agc 691Met Gly Leu Gly Gly Thr Arg Thr His Gly Cys Pro Asp Arg Cys Ser
185 190 195caa ggg tgc tgaggttacc gttctgtccc gtt 723Gln Gly Cys
200<210>246<211>200<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>246Val Ser Ile Ser Val Lys Ala Leu Gln Lys Ser Gly Pro Glu Ala Pro1 5 10 15Phe Glu Val Lys Ile Ile Glu Arg Arg Asp Pro Arg Ala Asp Asp Val
20 25 30Val Ile Asp Ile Lys Ala Ala Gly Ile Cys His Ser Asp Ile His Thr
35 40 45Ile Arg Asn Glu Trp Gly Glu Ala His Phe Pro Leu Thr Val Gly His
50 55 60Glu Ile Ala Gly Val Val Ser Ala Val Gly Ser Asp Val Thr Lys Trp65 70 75 80Lys Val Gly Asp Arg Val Gly Val Gly Cys Leu Val Asn Ser Cys Gly
85 90 95Glu Cys Glu Gln Cys Val Ala Gly Phe Glu Asn Asn Cys Leu Arg Gly
100 105 110Asn Val Gly Thr Tyr Asn Ser Asn Asp Val Asp Gly Thr Ile Thr Gln
115 120 125Gly Gly Tyr Ala Glu Lys Val Val Val Asn Glu Arg Phe Leu Cys Ser
130 135 140Ile Pro Glu Glu Leu Asn Phe Asp Val Ala Ala Pro Leu Leu Cys Ala145 150 155 160Gly Ile Thr Thr Tyr Ser Pro Ile Ala Arg Trp Asn Val Lys Glu Gly
165 170 175Asp Lys Val Ala Val Met Gly Leu Gly Gly Thr Arg Thr His Gly Cys
180 185 190Pro Asp Arg Cys Ser Gln Gly Cys
195 200<210>247<211>1338<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1315)<223>RXN02478<400>247gacatcgtcg aagcgctctc cagcggcaac atcgacgatt atcgcagcgc cgtgctcgct 60cactacgcgc cgtttcgccg catgatttcc aacatgctcg atg cgc act agc ctc 115
Met Arg Thr Ser Leu
1 5att gcg cgc ggg ttg tac cgc att ccc gcg ctg gtc tgg gat cag ggt 163Ile Ala Arg Gly Leu Tyr Arg Ile Pro Ala Leu Val Trp Asp Gln Gly
10 15 20ctt tta acg ctt ttc gac gcc cgc ctc agt gtt gac gac ctc ccc gca 211Leu Leu Thr Leu Phe Asp Ala Arg Leu Ser Val Asp Asp Leu Pro Ala
25 30 35ccc atc gac gtg gtg tca gcg cga tcc tca gac ggc atc acc tgg acc 259Pro Ile Asp Val Val Ser Ala Arg Ser Ser Asp Gly Ile Thr Trp Thr
40 45 50acc cca gaa cca gca atc gtc gaa act gaa cac cgc ggt gtg ggc gat 307Thr Pro Glu Pro Ala Ile Val Glu Thr Glu His Arg Gly Val Gly Asp
55 60 65gtc tgc ctt gtc acg ggc gat ctg tgc ttc cac gga ttg tcc aac ctc 355Val Cys Leu Val Thr Gly Asp Leu Cys Phe His Gly Leu Ser Asn Leu70 75 80 85gca gga ttt ttt gag gat ccc acc gac ctt gaa ccc cgg ctg gcg cgc 403Ala Gly Phe Phe Glu Asp Pro Thr Asp Leu Glu Pro Arg Leu Ala Arg
90 95 100cgg gat gtg agt ggg tgg acg tcg ata agc atg gcc cac tat ttt gcg 451Arg Asp Val Ser Gly Trp Thr Ser Ile Ser Met Ala His Tyr Phe Ala
105 110 115gat gtt gat gcc gcg ttc gcc tcg tcg ggg acg gga ctt gtc ctg gcg 499Asp Val Asp Ala Ala Phe Ala Ser Ser Gly Thr Gly Leu Val Leu Ala
120 125 130gat ggg cgg tgg att cag agt ttt gtg gtg cgg cgc ggg cgc gag att 547Asp Gly Arg Trp Ile Gln Ser Phe Val Val Arg Arg Gly Arg Glu Ile
135 140 145tcg ctt cgg att ctg cgc agc gat ggc cac atc acc gat att gcc ggc 595Ser Leu Arg Ile Leu Arg Ser Asp Gly His Ile Thr Asp Ile Ala Gly150 155 160 165ggt aac gaa tcc gcg atg acg cag ctg ccg agc ggt cgg att gtg ctg 643Gly Asn Glu Ser Ala Met Thr Gln Leu Pro Ser Gly Arg Ile Val Leu
170 175 180cat tcc agg ggg gtg gga cac cgt ctg agc agt gtg tcc gat gat ttc 691His Ser Arg Gly Val Gly His Arg Leu Ser Ser Val Ser Asp Asp Phe
185 190 195ggg gag aca ttc act ccg ctg gag cct gtg cct gaa cta atc gac ccc 739Gly Glu Thr Phe Thr Pro Leu Glu Pro Val Pro Glu Leu Ile Asp Pro
200 205 210ggc tgc aac ggc cac gtg ttc tac tgg aaa gcg gct gga atg ctc gcc 787Gly Cys Asn Gly His Val Phe Tyr Trp Lys Ala Ala Gly Met Leu Ala
215 220 225gca acg cac ctg gcg gac cct gat ctg cga cgc cac ttg gtg gtt gat 835Ala Thr His Leu Ala Asp Pro Asp Leu Arg Arg His Leu Val Val Asp230 235 240 245tta tcc agc gac gaa gga gcg acc tgg gcg cat cgc atc acc atc gag 883Leu Ser Ser Asp Glu Gly Ala Thr Trp Ala His Arg Ile Thr Ile Glu
250 255 260cgc gaa gaa gcc gcc tat tca acc gct gcg gaa atg ccc aac gga gat 931Arg Glu Glu Ala Ala Tyr Ser Thr Ala Ala Glu Met Pro Asn Gly Asp
265 270 275gtt gcc gtg gtg tgg gaa gca gag gga acg cgc gcg ata aaa tgc acg 979Val Ala Val Val Trp Glu Ala Glu Gly Thr Arg Ala Ile Lys Cys Thr
280 285 290gtg atc agc gta aat gat att tcg ctg cgg atc gat gag ccc att tcc 1027Val Ile Ser Val Asn Asp Ile Ser Leu Arg Ile Asp Glu Pro Ile Ser
295 300 305gat gcc ata tcc ctc cgc cat gtg gtg atc aac gat gac cat gac ggc 1075Asp Ala Ile Ser Leu Arg His Val Val Ile Asn Asp Asp His Asp Gly310 315 320 325atc gaa gtc gca ctg cct gac gca tcg caa tgg ggt gaa ggt gta ttc 1123Ile Glu Val Ala Leu Pro Asp Ala Ser Gln Trp Gly Glu Gly Val Phe
330 335 340aaa att gtg tcc aat cca gac gcg agc acc caa aaa atc cgc act cga 1171Lys Ile Val Ser Asn Pro Asp Ala Ser Thr Gln Lys Ile Arg Thr Arg
345 350 355ggc aag ccc gcg cga cag acc ctg gaa att ggg gat gaa ttg gtt ttt 1219Gly Lys Pro Ala Arg Gln Thr Leu Glu Ile Gly Asp Glu Leu Val Phe
360 365 370gat atc cgc aag ggt gga gaa gtg gct tac ggc gtc acg gtt cct tat 1267Asp Ile Arg Lys Gly Gly Glu Val Ala Tyr Gly Val Thr Val Pro Tyr
375 380 385gat ggt cgc tcg ttg ggg gaa gtt aaa cag gat ttt gga gtg ggg ctg 1315Asp Gly Arg Ser Leu Gly Glu Val Lys Gln Asp Phe Gly Val Gly Leu390 395 400 405tagaggccga tttgcggtcc ttt 1338<210>248<211>405<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>248Met Arg Thr Ser Leu Ile Ala Arg Gly Leu Tyr Arg Ile Pro Ala Leu1 5 10 15Val Trp Asp Gln Gly Leu Leu Thr Leu Phe Asp Ala Arg Leu Ser Val
20 25 30Asp Asp Leu Pro Ala Pro Ile Asp Val Val Ser Ala Arg Ser Ser Asp
35 40 45Gly Ile Thr Trp Thr Thr Pro Glu Pro Ala Ile Val Glu Thr Glu His
50 55 60Arg Gly Val Gly Asp Val Cys Leu Val Thr Gly Asp Leu Cys Phe His65 70 75 80Gly Leu Ser Asn Leu Ala Gly Phe Phe Glu Asp Pro Thr Asp Leu Glu
85 90 95Pro Arg Leu Ala Arg Arg Asp Val Ser Gly Trp Thr Ser Ile Ser Met
100 105 110Ala His Tyr Phe Ala Asp Val Asp Ala Ala Phe Ala Ser Ser Gly Thr
115 120 125Gly Leu Val Leu Ala Asp Gly Arg Trp Ile Gln Ser Phe Val Val Arg
130 135 140Arg Gly Arg Glu Ile Ser Leu Arg Ile Leu Arg Ser Asp Gly His Ile145 150 155 160Thr Asp Ile Ala Gly Gly Asn Glu Ser Ala Met Thr Gln Leu Pro Ser
165 170 175Gly Arg Ile Val Leu His Ser Arg Gly Val Gly His Arg Leu Ser Ser
180 185 190Val Ser Asp Asp Phe Gly Glu Thr Phe Thr Pro Leu Glu Pro Val Pro
195 200 205Glu Leu Ile Asp Pro Gly Cys Asn Gly His Val Phe Tyr Trp Lys Ala
210 215 220Ala Gly Met Leu Ala Ala Thr His Leu Ala Asp Pro Asp Leu Arg Arg225 230 235 240His Leu Val Val Asp Leu Ser Ser Asp Glu Gly Ala Thr Trp Ala His
245 250 255Arg Ile Thr Ile Glu Arg Glu Glu Ala Ala Tyr Ser Thr Ala Ala Glu
260 265 270Met Pro Asn Gly Asp Val Ala Val Val Trp Glu Ala Glu Gly Thr Arg
275 280 285Ala Ile Lys Cys Thr Val Ile Ser Val Asn Asp Ile Ser Leu Arg Ile
290 295 300Asp Glu Pro Ile Ser Asp Ala Ile Ser Leu Arg His Val Val Ile Asn305 310 315 320Asp Asp His Asp Gly Ile Glu Val Ala Leu Pro Asp Ala Ser Gln Trp
325 330 335Gly Glu Gly Val Phe Lys Ile Val Ser Asn Pro Asp Ala Ser Thr Gln
340 345 350Lys Ile Arg Thr Arg Gly Lys Pro Ala Arg Gln Thr Leu Glu Ile Gly
355 360 365Asp Glu Leu Val Phe Asp Ile Arg Lys Gly Gly Glu Val Ala Tyr Gly
370 375 380Val Thr Val Pro Tyr Asp Gly Arg Ser Leu Gly Glu Val Lys Gln Asp385 390 395 400Phe Gly Val Gly Leu
405<210>249<211>1213<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1213)<223>RXN00343<400>249ttcggtagaa tgggtaggtt gtcgtgcttg aggtgtggtg gataaccacc tctacaacac 60cacccaagct ctgttagaaa aaattgagga agcagtctaa atg aaa cac caa tat 115
Met Lys His Gln Tyr
1 5gat gtc atc gtt gtc ggt tcc ggc gct ggc gga tta tca gct gca gtc 163Asp Val Ile Val Val Gly Ser Gly Ala Gly Gly Leu Ser Ala Ala Val
10 15 20agt gca gct tac ggc ggt aag aaa gtc gct gta att gaa aag gcc tca 211Ser Ala Ala Tyr Gly Gly Lys Lys Val Ala Val Ile Glu Lys Ala Ser
25 30 35gta ctc ggt gga gcc acc acc tgg tcc ggc ggt tgg gct tgg act cct 259Val Leu Gly Gly Ala Thr Thr Trp Ser Gly Gly Trp Ala Trp Thr Pro
40 45 50gga acc agc ctt gcg cgc aaa gac gga gta gtg gaa tcc aaa gaa gaa 307Gly Thr Ser Leu Ala Arg Lys Asp Gly Val Val Glu Ser Lys Glu Glu
55 60 65ttc caa acc tac ctg caa gcg gta gtg ggg gag tac tac caa gaa gac 355Phe Gln Thr Tyr Leu Gln Ala Val Val Gly Glu Tyr Tyr Gln Glu Asp70 75 80 85aac atc tcc gcc ttc ttg gac gca gcc cct gaa atg gtc gat ttc ttt 403Asn Ile Ser Ala Phe Leu Asp Ala Ala Pro Glu Met Val Asp Phe Phe
90 95 100gaa aaa aac acc gac ctg cag tgg acc ccc ggc gcg aaa atc aac gac 451Glu Lys Asn Thr Asp Leu Gln Trp Thr Pro Gly Ala Lys Ile Asn Asp
105 110 115atc tac ggc aac ctc ccc ggt gct ggc act gga cac cgc tcc gtt ggg 499Ile Tyr Gly Asn Leu Pro Gly Ala Gly Thr Gly His Arg Ser Val Gly
120 125 130cca aaa cca ttc aac gga cgc aaa gta ccc aag agt gtt ctt cca aaa 547Pro Lys Pro Phe Asn Gly Arg Lys Val Pro Lys Ser Val Leu Pro Lys
135 140 145ctg cgc cac cag ctg tat gaa acc tcc ttc ctg gga atg ggc atc atg 595Leu Arg His Gln Leu Tyr Glu Thr Ser Phe Leu Gly Met Gly Ile Met150 155 160 165gct ggg cct gac ctg acg aaa ttc ctc tct gct tca cag ttc gat cca 643Ala Gly Pro Asp Leu Thr Lys Phe Leu Ser Ala Ser Gln Phe Asp Pro
170 175 180cgt ggt tgg gta cat gcc gcc agg cgc gtc atc gtg cac atg tgg gac 691Arg Gly Trp Val His Ala Ala Arg Arg Val Ile Val His Met Trp Asp
185 190 195atg gtc gtg cac aaa cgc aat atg cag atg gtc aac ggt gca gca ctc 739Met Val Val His Lys Arg Asn Met Gln Met Val Asn Gly Ala Ala Leu
200 205 210acc gct cga ctg gct acc tct gca gac aag ctg ggc gtt gat ctc ctg 787Thr Ala Arg Leu Ala Thr Ser Ala Asp Lys Leu Gly Val Asp Leu Leu
215 220 225gtc aat cac tcc gca gtg tcg ttg aat tac aaa aac gac cgc gtt acc 835Val Asn His Ser Ala Val Ser Leu Asn Tyr Lys Asn Asp Arg Val Thr230 235 240 245ggc gtg aaa gta caa acc cca cag ggc ttg gta gat ttc gaa gcc act 883Gly Val Lys Val Gln Thr Pro Gln Gly Leu Val Asp Phe Glu Ala Thr
250 255 260gcc ggc gtc gtg ctc gcc act ggt gga ttc ccc aac aac gtt gac ctg 931Ala Gly Val Val Leu Ala Thr Gly Gly Phe Pro Asn Asn Val Asp Leu
265 270 275cgc aag gaa ctc ttc cca cgc acc cca tca ggt caa gaa cac tgg acc 979Arg Lys Glu Leu Phe Pro Arg Thr Pro Ser Gly Gln Glu His Trp Thr
280 285 290ctc gcg cca gca gaa acc acc ggc gac gga cta tcc atg gct cgg gaa 1027Leu Ala Pro Ala Glu Thr Thr Gly Asp Gly Leu Ser Met Ala Arg Glu
295 300 305atc ggt gca ggt ttt gtc aac gac ctg aaa tcc cca gca gca tgg tgc 1075Ile Gly Ala Gly Phe Val Asn Asp Leu Lys Ser Pro Ala Ala Trp Cys310 315 320 325cct gtt tca ttg gtc cca tac ttc aac gga aaa gtc ggc acc ttc ccc 1123Pro Val Ser Leu Val Pro Tyr Phe Asn Gly Lys Val Gly Thr Phe Pro
330 335 340cac atc atg gac cgc gca aaa cca ggc tcc atc ggt gtt gtc tcc aca 1171His Ile Met Asp Arg Ala Lys Pro Gly Ser Ile Gly Val Val Ser Thr
345 350 355ggt aag cga ttc gtc aat gaa gcc aac ggc tac tac gac tac 1213Gly Lys Arg Phe Val Asn Glu Ala Asn Gly Tyr Tyr Asp Tyr
360 365 370<210>250<211>371<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>250Met Lys His Gln Tyr Asp Val Ile Val Val Gly Ser Gly Ala Gly Gly1 5 10 15Leu Ser Ala Ala Val Ser Ala Ala Tyr Gly Gly Lys Lys Val Ala Val
20 25 30Ile Glu Lys Ala Ser Val Leu Gly Gly Ala Thr Thr Trp Ser Gly Gly
35 40 45Trp Ala Trp Thr Pro Gly Thr Ser Leu Ala Arg Lys Asp Gly Val Val
50 55 60Glu Ser Lys Glu Glu Phe Gln Thr Tyr Leu Gln Ala Val Val Gly Glu65 70 75 80Tyr Tyr Gln Glu Asp Asn Ile Ser Ala Phe Leu Asp Ala Ala Pro Glu
85 90 95Met Val Asp Phe Phe Glu Lys Asn Thr Asp Leu Gln Trp Thr Pro Gly
100 105 110Ala Lys Ile Asn Asp Ile Tyr Gly Asn Leu Pro Gly Ala Gly Thr Gly
115 120 125His Arg Ser Val Gly Pro Lys Pro Phe Asn Gly Arg Lys Val Pro Lys
130 135 140Ser Val Leu Pro Lys Leu Arg His Gln Leu Tyr Glu Thr Ser Phe Leu145 150 155 160Gly Met Gly Ile Met Ala Gly Pro Asp Leu Thr Lys Phe Leu Ser Ala
165 170 175Ser Gln Phe Asp Pro Arg Gly Trp Val His Ala Ala Arg Arg Val Ile
180 185 190Val His Met Trp Asp Met Val Val His Lys Arg Asn Met Gln Met Val
195 200 205Asn Gly Ala Ala Leu Thr Ala Arg Leu Ala Thr Ser Ala Asp Lys Leu
210 215 220Gly Val Asp Leu Leu Val Asn His Ser Ala Val Ser Leu Asn Tyr Lys225 230 235 240Asn Asp Arg Val Thr Gly Val Lys Val Gln Thr Pro Gln Gly Leu Val
245 250 255Asp Phe Glu Ala Thr Ala Gly Val Val Leu Ala Thr Gly Gly Phe Pro
260 265 270Asn Asn Val Asp Leu Arg Lys Glu Leu Phe Pro Arg Thr Pro Ser Gly
275 280 285Gln Glu His Trp Thr Leu Ala Pro Ala Glu Thr Thr Gly Asp Gly Leu
290 295 300Ser Met Ala Arg Glu Ile Gly Ala Gly Phe Val Asn Asp Leu Lys Ser305 310 315 320Pro Ala Ala Trp Cys Pro Val Ser Leu Val Pro Tyr Phe Asn Gly Lys
325 330 335Val Gly Thr Phe Pro His Ile Met Asp Arg Ala Lys Pro Gly Ser Ile
340 345 350Gly Val Val Ser Thr Gly Lys Arg Phe Val Asn Glu Ala Asn Gly Tyr
355 360 365Tyr Asp Tyr
370<210>251<211>1083<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1060)<223>RXN01555<400>251ctggttttgt gccgaggatc agccagcaag ttaatgatcc ttacatggcg ctgttgttgg 60cgcggtagtc aatcatgggg gagtatccca ccgtatccgc gtg aac aag ggc gtg 115
Val Asn Lys Gly Val
1 5gtg ctg gta gca ggt gga ttc tcc cag aat cca gaa ctg cgc atg aag 163Val Leu Val Ala Gly Gly Phe Ser Gln Asn Pro Glu Leu Arg Met Lys
10 15 20tac atg cca gaa ccc acc cca cag ttc tcc cgc acc aac gaa agc gcc 211Tyr Met Pro Glu Pro Thr Pro Gln Phe Ser Arg Thr Asn Glu Ser Ala
25 30 35acc ggc gac acc atg gcc ctt gct gcg aaa gtg gga gca cgc cta ggc 259Thr Gly Asp Thr Met Ala Leu Ala Ala Lys Val Gly Ala Arg Leu Gly
40 45 50gac gac aac ggt gaa aac gca ctg tgg ttc cca tcg tcc atc ggc acc 307Asp Asp Asn Gly Glu Asn Ala Leu Trp Phe Pro Ser Ser Ile Gly Thr
55 60 65cgc gcc gac gga tcc acc gcg gtg tac cca cac att tgg gac cgt ggc 355Arg Ala Asp Gly Ser Thr Ala Val Tyr Pro His Ile Trp Asp Arg Gly70 75 80 85cgc ctc gga gtc atc gca gtc aac gca gca ggc gag cgt ttc gtc gat 403Arg Leu Gly Val Ile Ala Val Asn Ala Ala Gly Glu Arg Phe Val Asp
90 95 100gaa tcG gtc tcc tac cac cgc ttc gtg cgc gcc atg tac gaa tcc aac 451Glu Ser Val Ser Tyr His Arg Phe Val Arg Ala Met Tyr Glu Ser Asn
105 110 115aaa acc acc ccg act gtt tca gcc tgg ctc att gtt gat tcc cac acc 499Lys Thr Thr Pro Thr Val Ser Ala Trp Leu Ile Val Asp Ser His Thr
120 125 130ctg gca aaa tac ggc ctc ggc atg atc acc atg cca cac ctg cct aaa 547Leu Ala Lys Tyr Gly Leu Gly Met Ile Thr Met Pro His Leu Pro Lys
135 140 145ctc gct ctg caa aaa tac atc gac tcc gga tac ctg cac gca gga tca 595Leu Ala Leu Gln Lys Tyr Ile Asp Ser Gly Tyr Leu His Ala Gly Ser150 155 160 165tcc ttg gat gaa ttg gca cgc agc att ggt gtg gac gct cgc ggc ctg 643Ser Leu Asp Glu Leu Ala Arg Ser Ile Gly Val Asp Ala Arg Gly Leu
170 175 180gaa caa acc gtc aaa cgc tac aat acc ttc gct aaa acg ggt atc gac 691Glu Gln Thr Val Lys Arg Tyr Asn Thr Phe Ala Lys Thr Gly Ile Asp
185 190 195gaa gac ttc cac aag ggc gaa ctc ctc ttc ggt caa gcc gcc ggc gat 739Glu Asp Phe His Lys Gly Glu Leu Leu Phe Gly Gln Ala Ala Gly Asp
200 205 210cca gac aac aag cca aac ccc aac gtc gga cca atc aag aag gga ccg 787Pro Asp Asn Lys Pro Asn Pro Asn Val Gly Pro Ile Lys Lys Gly Pro
215 220 225ttc tac gca atc gct gta gtc cca acc cct ctg gcc act gcc ttt ggc 835Phe Tyr Ala Ile Ala Val Val Pro Thr Pro Leu Ala Thr Ala Phe Gly230 235 240 245atc agc atc aac ccc aac gga cag gtt gtt agt gaa gat ggg gag ccc 883Ile Ser Ile Asn Pro Asn Gly Gln Val Val Ser Glu Asp Gly Glu Pro
250 255 260atc att gga ctg tac tcc gca gga aat gat gcc caa tct gtc atg gct 931Ile Ile Gly Leu Tyr Ser Ala Gly Asn Asp Ala Gln Ser Val Met Ala
265 270 275tct gaa tat cct ggt gct ggt tca cag gtt ggt tcc gga atg acc ttt 979Ser Glu Tyr Pro Gly Ala Gly Ser Gln Val Gly Ser Gly Met Thr Phe
280 285 290ggt tgg atc gca gca cag cac gcg gtg ggg aaa gcg gga aaa tcc gga 1027Gly Trp Ile Ala Ala Gln His Ala Val Gly Lys Ala Gly Lys Ser Gly
295 300 305gga gct aag gca gga tat gcc gcg tct tct aag taattgcttg gtgggttgct 1080Gly Ala Lys Ala Gly Tyr Ala Ala Ser Ser Lys310 315 320tac1083<210>252<211>320<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>252Val Asn Lys Gly Val Val Leu Val Ala Gly Gly Phe Ser Gln Asn Pro1 5 10 15Glu Leu Arg Met Lys Tyr Met Pro Glu Pro Thr Pro Gln Phe Ser Arg
20 25 30Thr Asn Glu Ser Ala Thr Gly Asp Thr Met Ala Leu Ala Ala Lys Val
35 40 45Gly Ala Arg Leu Gly Asp Asp Asn Gly Glu Asn Ala Leu Trp Phe Pro
50 55 60Ser Ser Ile Gly Thr Arg Ala Asp Gly Ser Thr Ala Val Tyr Pro His65 70 75 80Ile Trp Asp Arg Gly Arg Leu Gly Val Ile Ala Val Asn Ala Ala Gly
85 90 95Glu Arg Phe Val Asp Glu Ser Val Ser Tyr His Arg Phe Val Arg Ala
100 105 110Met Tyr Glu Ser Asn Lys Thr Thr Pro Thr Val Ser Ala Trp Leu Ile
115 120 125Val Asp Ser His Thr Leu Ala Lys Tyr Gly Leu Gly Met Ile Thr Met
130 135 140Pro His Leu Pro Lys Leu Ala Leu Gln Lys Tyr Ile Asp Ser Gly Tyr145 150 155 160Leu His Ala Gly Ser Ser Leu Asp Glu Leu Ala Arg Ser Ile Gly Val
165 170 175Asp Ala Arg Gly Leu Glu Gln Thr Val Lys Arg Tyr Asn Thr Phe Ala
180 185 190Lys Thr Gly Ile Asp Glu Asp Phe His Lys Gly Glu Leu Leu Phe Gly
195 200 205Gln Ala Ala Gly Asp Pro Asp Asn Lys Pro Asn Pro Asn Val Gly Pro
210 215 220Ile Lys Lys Gly Pro Phe Tyr Ala Ile Ala Val Val Pro Thr Pro Leu225 230 235 240Ala Thr Ala Phe Gly Ile Ser Ile Asn Pro Asn Gly Gln Val Val Ser
245 250 255Glu Asp Gly Glu Pro Ile Ile Gly Leu Tyr Ser Ala Gly Asn Asp Ala
260 265 270Gln Ser Val Met Ala Ser Glu Tyr Pro Gly Ala Gly Ser Gln Val Gly
275 280 285Ser Gly Met Thr Phe Gly Trp Ile Ala Ala Gln His Ala Val Gly Lys
290 295 300Ala Gly Lys Ser Gly Gly Ala Lys Ala Gly Tyr Ala Ala Ser Ser Lys305 310 315 320<210>253<211>1428<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1405)<223>RXN01166<400>253accgtaccca cagacacacc agaattaaca gaaacagact gaaaaacaac atcgctcgac 60atgcgcgtaa tcctaacccg cgcacactaa tgtggccgat atg ggc tac acc aac 115
Met Gly Tyr Thr Asn
1 5ctc aac gac aca cgg gtc ttg cgc gcc ggg tca tgt gat gcc tgg tgg 163Leu Asn Asp Thr Arg Val Leu Arg Ala Gly Ser Cys Asp Ala Trp Trp
10 15 20cgc acg atg tct ccg cta gtg cag cag gga agt gag gca gtc ttt cgg 211Arg Thr Met Ser Pro Leu Val Gln Gln Gly Ser Glu Ala Val Phe Arg
25 30 35cgc atc atg ggt ctc tcg cgg cgt cct gat cgg aaa cct ggc ttt gac 259Arg Ile Met Gly Leu Ser Arg Arg Pro Asp Arg Lys Pro Gly Phe Asp
40 45 50gat gtc cca cat ttc ggc gca gct gtt cga gtt ccc ggt cta aaa cac 307Asp Val Pro His Phe Gly Ala Ala Val Arg Val Pro Gly Leu Lys His
55 60 65ggc acg ttg gtc aat gct gca ccc ttg aaa gtt ttg ggc gca cgg ggc 355Gly Thr Leu Val Asn Ala Ala Pro Leu Lys Val Leu Gly Ala Arg Gly70 75 80 85gag ccc aac ccc gcg agt tcg tac cgt ttt gaa tac atc acc ggt gat 403Glu Pro Asn Pro Ala Ser Ser Tyr Arg Phe Glu Tyr Ile Thr Gly Asp
90 95 100tcc gca ggt cga gcc atc act gcg acc ggc gct gtc ctc ttt tcc aca 451Ser Ala Gly Arg Ala Ile Thr Ala Thr Gly Ala Val Leu Phe Ser Thr
105 110 115cgc ccc tgg aca acc ggc ccg cgt ccc gcg atc gcc atg gct cca tcc 499Arg Pro Trp Thr Thr Gly Pro Arg Pro Ala Ile Ala Met Ala Pro Ser
120 125 130acc caa ggc gtc gca cag cac tgc gat ccc tcc cac acc tgc gcc atc 547Thr Gln Gly Val Ala Gln His Cys Asp Pro Ser His Thr Cys Ala Ile
135 140 145gga ctc aac gca ttc tat gac aaa ccc ttc gac gca atc att gct tac 595Gly Leu Asn Ala Phe Tyr Asp Lys Pro Phe Asp Ala Ile Ile Ala Tyr150 155 160 165gaa ctc ccc gtc atc ctc tgg ttt cta gct cac gga ctt gac gtt gtg 643Glu Leu Pro Val Ile Leu Trp Phe Leu Ala His Gly Leu Asp Val Val
170 175 180ttc atc gat tac ccc cgc gac ccc gca acc ggc gtc caa tac tat tgc 691Phe Ile Asp Tyr Pro Arg Asp Pro Ala Thr Gly Val Gln Tyr Tyr Cys
185 190 195gat tcc atc gct gca gct aaa tcg ctt ctc gac gcc gtc ctc gcc tcc 739Asp Ser Ile Ala Ala Ala Lys Ser Leu Leu Asp Ala Val Leu Ala Ser
200 205 210aga caa ctc ggc ctt tca ccg gaa gca ccg ctt ggc ctg tgg gga ttc 787Arg Gln Leu Gly Leu Ser Pro Glu Ala Pro Leu Gly Leu Trp Gly Phe
215 220 225tcc caa gga ggc ggc gcc act ggc tgg gct gca caa ttg cag gat tac 835Ser Gln Gly Gly Gly Ala Thr Gly Trp Ala Ala Gln Leu Gln Asp Tyr230 235 240 245gca cct gat gtc cgc cca aag gca gcg gtc gtg ggc gct cca cca gtg 883Ala Pro Asp Val Arg Pro Lys Ala Ala Val Val Gly Ala Pro Pro Val
250 255 260gat ctc ttc cgc gtc ttg gac act gtc gac ggc gga ttg ctc acc gga 931Asp Leu Phe Arg Val Leu Asp Thr Val Asp Gly Gly Leu Leu Thr Gly
265 270 275gtg att gcc tac gcc atc gcg gga ctt gca gtg aac tct tca gag atg 979Val Ile Ala Tyr Ala Ile Ala Gly Leu Ala Val Asn Ser Ser Glu Met
280 285 290ttt gag gaa atc atg tcg gtg tta aat gaa cgc gga gtc agt gat gtg 1027Phe Glu Glu Ile Met Ser Val Leu Asn Glu Arg Gly Val Ser Asp Val
295 300 305ctg aaa aat atc acc agc tgc gcg gga ggt tcc ttg ttg gcc agt ggc 1075Leu Lys Asn Ile Thr Ser Cys Ala Gly Gly Ser Leu Leu Ala Ser Gly310 315 320 325tac tcg tct tcc cgc ggg tgg aca cat cag ggc acg ccg ctg gca gac 1123Tyr Ser Ser Ser Arg Gly Trp Thr His Gln Gly Thr Pro Leu Ala Asp
330 335 340att ctg gac gat ctg cca ctt gtt gtc gct gag ttt ggg aag caa aag 1171Ile Leu Asp Asp Leu Pro Leu Val Val Ala Glu Phe Gly Lys Gln Lys
345 350 355ctg ggt cgt gtg gcg cca gaa atc cca gtg ctg ttg tgg ggc tct aaa 1219Leu Gly Arg Val Ala Pro Glu Ile Pro Val Leu Leu Trp Gly Ser Lys
360 365 370aat gat gat gtc att ccc att gat ccc att agg gaa ttg cgt gat agc 1267Asn Asp Asp Val Ile Pro Ile Asp Pro Ile Arg Glu Leu Arg Asp Ser
375 380 385tgg gcg gac aag ggt acg cca ttg acc tgg cat gaa tcc caa gcg ccg 1315Trp Ala Asp Lys Gly Thr Pro Leu Thr Trp His Glu Ser Gln Ala Pro390 395 400 405cgt gtg cca gga cgc aca ggt ctc aac cat ttc ggg ccc tat ttt aga 1363Arg Val Pro Gly Arg Thr Gly Leu Asn His Phe Gly Pro Tyr Phe Arg
410 415 420aac ctg gaa aag tac tcg gga tgg ctc ata gat cat ctt gtc 1405Asn Leu Glu Lys Tyr Ser Gly Trp Leu Ile Asp His Leu Val
425 430 435tgagtgccgt tttaaaggct cgg 1428<210>254<211>435<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>254Met Gly Tyr Thr Asn Leu Asn Asp Thr Arg Val Leu Arg Ala Gly Ser1 5 10 15Cys Asp Ala Trp Trp Arg Thr Met Ser Pro Leu Val Gln Gln Gly Ser
20 25 30Glu Ala Val Phe Arg Arg Ile Met Gly Leu Ser Arg Arg Pro Asp Arg
35 40 45Lys Pro Gly Phe Asp Asp Val Pro His Phe Gly Ala Ala Val Arg Val
50 55 60Pro Gly Leu Lys His Gly Thr Leu Val Asn Ala Ala Pro Leu Lys Val65 70 75 80Leu Gly Ala Arg Gly Glu Pro Asn Pro Ala Ser Ser Tyr Arg Phe Glu
85 90 95Tyr Ile Thr Gly Asp Ser Ala Gly Arg Ala Ile Thr Ala Thr Gly Ala
100 105 110Val Leu Phe Ser Thr Arg Pro Trp Thr Thr Gly Pro Arg Pro Ala Ile
115 120 125Ala Met Ala Pro Ser Thr Gln Gly Val Ala Gln His Cys Asp Pro Ser
130 135 140His Thr Cys Ala Ile Gly Leu Asn Ala Phe Tyr Asp Lys Pro Phe Asp145 150 155 160Ala Ile Ile Ala Tyr Glu Leu Pro Val Ile Leu Trp Phe Leu Ala His
165 170 175Gly Leu Asp Val Val Phe Ile Asp Tyr Pro Arg Asp Pro Ala Thr Gly
180 185 190Val Gln Tyr Tyr Cys Asp Ser Ile Ala Ala Ala Lys Ser Leu Leu Asp
195 200 205Ala Val Leu Ala Ser Arg Gln Leu Gly Leu Ser Pro Glu Ala Pro Leu
210 215 220Gly Leu Trp Gly Phe Ser Gln Gly Gly Gly Ala Thr Gly Trp Ala Ala225 230 235 240Gln Leu Gln Asp Tyr Ala Pro Asp Val Arg Pro Lys Ala Ala Val Val
245 250 255Gly Ala Pro Pro Val Asp Leu Phe Arg Val Leu Asp Thr Val Asp Gly
260 265 270Gly Leu Leu Thr Gly Val Ile Ala Tyr Ala Ile Ala Gly Leu Ala Val
275 280 285Asn Ser Ser Glu Met Phe Glu Glu Ile Met Ser Val Leu Asn Glu Arg
290 295 300Gly Val Ser Asp Val Leu Lys Asn Ile Thr Ser Cys Ala Gly Gly Ser305 310 315 320Leu Leu Ala Ser Gly Tyr Ser Ser Ser Arg Gly Trp Thr His Gln Gly
325 330 335Thr Pro Leu Ala Asp Ile Leu Asp Asp Leu Pro Leu Val Val Ala Glu
340 345 350Phe Gly Lys Gln Lys Leu Gly Arg Val Ala Pro Glu Ile Pro Val Leu
355 360 365Leu Trp Gly Ser Lys Asn Asp Asp Val Ile Pro Ile Asp Pro Ile Arg
370 375 380Glu Leu Arg Asp Ser Trp Ala Asp Lys Gly Thr Pro Leu Thr Trp His385 390 395 400Glu Ser Gln Ala Pro Arg Val Pro Gly Arg Thr Gly Leu Asn His Phe
405 410 415Gly Pro Tyr Phe Arg Asn Leu Glu Lys Tyr Ser Gly Trp Leu Ile Asp
420 425 430His Leu Val
435<210>255<211>1281<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1258)<223>RXN02001<400>255gcggtttcgt catggataag gactgtgttc gggaccattg cgatactcgt gtcaaaaggc 60gatagtccag catagaccgt gctttatcga aggtgaaccc atg ccc gtt atc aat 115
Met Pro Val Ile Asn
1 5agt atc gcc agt ttt tcc gac gag atg acc cgc tgg cgg cgt cac ctg 163Ser Ile Ala Ser Phe Ser Asp Glu Met Thr Arg Trp Arg Arg His Leu
10 15 20cat caa aac ccc gaa atc agc ttt gat tgt gtg gaa act gcg gcc ttc 211His Gln Asn Pro Glu Ile Ser Phe Asp Cys Val Glu Thr Ala Ala Phe
25 30 35gtg gcc gag cag ctg cgc agc ttc ggg gtg gat gaa att cac acc ggc 259Val Ala Glu Gln Leu Arg Ser Phe Gly Val Asp Glu Ile His Thr Gly
40 45 50atc gcg aaa acc ggt atc atc gcc ctg att cac ggg cgc gag gct ggc 307Ile Ala Lys Thr Gly Ile Ile Ala Leu Ile His Gly Arg Glu Ala Gly
55 60 65ccc gtc gtc ggc ctg cgc gcc gat atg gac gcg ctg ccg ctg acc gag 355Pro Val Val Gly Leu Arg Ala Asp Met Asp Ala Leu Pro Leu Thr Glu70 75 80 85att acc ggc gtc gac tat gcc tcg acc acc ccc gga aaa atg cac gcc 403Ile Thr Gly Val Asp Tyr Ala Ser Thr Thr Pro Gly Lys Met His Ala
90 95 100tgc ggc cac gac ggc cac acg acc atg ctg ctg ggc gcc gcc aaa tat 451Cys Gly His Asp Gly His Thr Thr Met Leu Leu Gly Ala Ala Lys Tyr
105 110 115ctg gcc gag acg cgc aat ttc gca ggt acc gtc gcg ctg atc ttc cag 499Leu Ala Glu Thr Arg Asn Phe Ala Gly Thr Val Ala Leu Ile Phe Gln
120 125 130cct gcg gaa gaa aac ggc ggc ggc gcg ggc gtt atg gtc gat gaa ggc 547Pro Ala Glu Glu Asn Gly Gly Gly Ala Gly Val Met Val Asp Glu Gly
135 140 145gtc ctc gac cgc ttt gcc atc gcc gaa gtc tac gcc ctg cac aac cag 595Val Leu Asp Arg Phe Ala Ile Ala Glu Val Tyr Ala Leu His Asn Gln150 155 160 165ccc ggc ctg ccg ctt ggc cat ttt atg acg aca gcc ggc ccg atc atg 643Pro Gly Leu Pro Leu Gly His Phe Met Thr Thr Ala Gly Pro Ile Met
170 175 180gcc gct gtc gac acg ttc gac atc aac att acc gga cgc ggc ggc cac 691Ala Ala Val Asp Thr Phe Asp Ile Asn Ile Thr Gly Arg Gly Gly His
185 190 195ggt gcc aaa ccg cac caa acc cgc gac ccc atc gtc gca gcc gtc gga 739Gly Ala Lys Pro His Gln Thr Arg Asp Pro Ile Val Ala Ala Val Gly
200 205 210att gtc caa gcg ttt caa acg ata gtc agc cgg aat cac aat ccg gtc 787Ile Val Gln Ala Phe Gln Thr Ile Val Ser Arg Asn His Asn Pro Val
215 220 225gag gac ctt gtc gtg tcg gtc acg caa atc cac acc ggc agc gcc gat 835Glu Asp Leu Val Val Ser Val Thr Gln Ile His Thr Gly Ser Ala Asp230 235 240 245aat atc atc ccc gaa acc gcc tat atc aac ggc act gtc cgc acc ttc 883Asn Ile Ile Pro Glu Thr Ala Tyr Ile Asn Gly Thr Val Arg Thr Phe
250 255 260aac aaa gac gtg cag gcc atg gtc atc acg cgg atg gaa gaa atc gtc 931Asn Lys Asp Val Gln Ala Met Val Ile Thr Arg Met Glu Glu Ile Val
265 270 275gcg ggc caa gct gca gcc tat ggg gtc gag gcg acg ctg acc tac aac 979Ala Gly Gln Ala Ala Ala Tyr Gly Val Glu Ala Thr Leu Thr Tyr Asn
280 285 290cgc aac tat ccc gcc acc att aac gac gcc gcc aaa gcc gcc atc gct 1027Arg Asn Tyr Pro Ala Thr Ile Asn Asp Ala Ala Lys Ala Ala Ile Ala
295 300 305gcc gaa gtc gcg ggc gag gtc ggc ctc ggg gtc aac ccg aac ggc tcg 1075Ala Glu Val Ala Gly Glu Val Gly Leu Gly Val Asn Pro Asn Gly Ser310 315 320 325cgc ggg atg ggg gcc gag gat ttc tcg tat ttc ctc gaa aag cgc ccg 1123Arg Gly Met Gly Ala Glu Asp Phe Ser Tyr Phe Leu Glu Lys Arg Pro
330 335 340ggt gcc tac ctg ttc gtc ggt aat ggc gac agc gcg ggc ctt cac aac 1171Gly Ala Tyr Leu Phe Val Gly Asn Gly Asp Ser Ala Gly Leu His Asn
345 350 355ccc gcc tat aat ttc aac gac gag gcc gcg ccc tac ggc gca tcg ttc 1219Pro Ala Tyr Asn Phe Asn Asp Glu Ala Ala Pro Tyr Gly Ala Ser Phe
360 365 370ttg gcc cgc atg gca gaa cgc ccc ttg ccg tta aag ggc tgatccatgg 1268Leu Ala Arg Met Ala Glu Arg Pro Leu Pro Leu Lys Gly
375 380 385cgctcgaaga tgc 1281<210>256<211>386<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>256Met Pro Val Ile Asn Ser Ile Ala Ser Phe Ser Asp Glu Met Thr Arg1 5 10 15Trp Arg Arg His Leu His Gln Asn Pro Glu Ile Ser Phe Asp Cys Val
20 25 30Glu Thr Ala Ala Phe Val Ala Glu Gln Leu Arg Ser Phe Gly Val Asp
35 40 45Glu Ile His Thr Gly Ile Ala Lys Thr Gly Ile Ile Ala Leu Ile His
50 55 60Gly Arg Glu Ala Gly Pro Val Val Gly Leu Arg Ala Asp Met Asp Ala65 70 75 80Leu Pro Leu Thr Glu Ile Thr Gly Val Asp Tyr Ala Ser Thr Thr Pro
85 90 95Gly Lys Met His Ala Cys Gly His Asp Gly His Thr Thr Met Leu Leu
100 105 110Gly Ala Ala Lys Tyr Leu Ala Glu Thr Arg Asn Phe Ala Gly Thr Val
115 120 125Ala Leu Ile Phe Gln Pro Ala Glu Glu Asn Gly Gly Gly Ala Gly Val
130 135 140Met Val Asp Glu Gly Val Leu Asp Arg Phe Ala Ile Ala Glu Val Tyr145 150 155 160Ala Leu His Asn Gln Pro Gly Leu Pro Leu Gly His Phe Met Thr Thr
165 170 175Ala Gly Pro Ile Met Ala Ala Val Asp Thr Phe Asp Ile Asn Ile Thr
180 185 190Gly Arg Gly Gly His Gly Ala Lys Pro His Gln Thr Arg Asp Pro Ile
195 200 205Val Ala Ala Val Gly Ile Val Gln Ala Phe Gln Thr Ile Val Ser Arg
210 215 220Asn His Asn Pro Val Glu Asp Leu Val Val Ser Val Thr Gln Ile His225 230 235 240Thr Gly Ser Ala Asp Asn Ile Ile Pro Glu Thr Ala Tyr Ile Asn Gly
245 250 255Thr Val Arg Thr Phe Asn Lys Asp Val Gln Ala Met Val Ile Thr Arg
260 265 270Met Glu Glu Ile Val Ala Gly Gln Ala Ala Ala Tyr Gly Val Glu Ala
275 280 285Thr Leu Thr Tyr Asn Arg Asn Tyr Pro Ala Thr Ile Asn Asp Ala Ala
290 295 300Lys Ala Ala Ile Ala Ala Glu Val Ala Gly Glu Val Gly Leu Gly Val305 310 315 320Asn Pro Asn Gly Ser Arg Gly Met Gly Ala Glu Asp Phe Ser Tyr Phe
325 330 335Leu Glu Lys Arg Pro Gly Ala Tyr Leu Phe Val Gly Asn Gly Asp Ser
340 345 350Ala Gly Leu His Asn Pro Ala Tyr Asn Phe Asn Asp Glu Ala Ala Pro
355 360 365Tyr Gly Ala Ser Phe Leu Ala Arg Met Ala Glu Arg Pro Leu Pro Leu
370 375 380Lys Gly385<210>257<211>570<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(547)<223>RXN03145<400>257agaggtgttt aaattcccgc ctctgcccca gatttaaaac tatgatgagg gatagcttct 60caattttcca ggttttccaa aatgaaagag gttcacgcac atg cct act tat act 115
Met Pro Thr Tyr Thr
1 5tgt tgg tcg caa aga att cgc att tct agg gaa gcc aag caa cgc atc 163Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala Lys Gln Arg Ile
10 15 20gct gag gca atc acc gat gcc cac cat gaa tta gcg cat gct ccc aag 211Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala His Ala Pro Lys
25 30 35tat ttg gtg cag gtg att ttc aat gag gtg gag cct gat tct tat ttc 259Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro Asp Ser Tyr Phe
40 45 50att gcg gcg cag tcg gcg tcg gaa aac cac att tgg gtc caa gca acg 307Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp Val Gln Ala Thr
55 60 65att cgt tcg ggg cgt aca gag aag caa aaa gag gaa ctt ctg ctt cgg 355Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu Leu Leu Leu Arg70 75 80 85ctg aca caa gag atc gcg ctg att ctt ggg atc ccc aat gaa gaa gta 403Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro Asn Glu Glu Val
90 95 100tgg gta tat ata ccg gag att cct ggt tcc aat atg acg gaa tat ggc 451Trp Val Tyr Ile Pro Glu Ile Pro Gly Ser Asn Met Thr Glu Tyr Gly
105 110 115cgt ctc ctc atg gaa cct ggc gaa gag gag aag tgg ttt aat tcg ctt 499Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp Phe Asn Ser Leu
120 125 130ccc gaa ggc ctg cgg gaa agg ttg acc gag cta gaa gga tcg tca gaa 547Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu Gly Ser Ser Glu
135 140 145tagctctcga ataggccatt tct 570<210>258<211>149<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>258Met Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu1 5 10 15Ala Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu
20 25 30Ala His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu
35 40 45Pro Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile
50 55 60Trp Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu65 70 75 80Glu Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile
85 90 95Pro Asn Glu Glu Val Trp Val Tyr Ile Pro Glu Ile Pro Gly Ser Asn
100 105 110Met Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys
115 120 125Trp Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu
130 135 140Glu Gly Ser Ser Glu145<210>259<211>1083<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1060)<223>RXN01466<400>259aatccatgat cccaaactac ctcaaagcgc ttgtaggcta agacttatgg atacacaacg 60cggctcattg cggggaaaag ctcataaagc aaggctaaag atg acg cca aat ggt 115
Met Thr Pro Asn Gly
1 5cgc agg caa ctc ctc ctg gag cgt ggc gca gca ttt agc aaa aac cgt 163Arg Arg Gln Leu Leu Leu Glu Arg Gly Ala Ala Phe Ser Lys Asn Arg
10 15 20acc ccg ggt cta aaa cac gtc gac cgc cac acc atc gtg gac tcc gac 211Thr Pro Gly Leu Lys His Val Asp Arg His Thr Ile Val Asp Ser Asp
25 30 35ggc ctc agc atc cac acg tac atg gtt ggc cat gcc gaa aat gcc acg 259Gly Leu Ser Ile His Thr Tyr Met Val Gly His Ala Glu Asn Ala Thr
40 45 50gca acg gtc gtg ttc atc cac ggc ttc acc ctc gcc gcc gaa gtg tat 307Ala Thr Val Val Phe Ile His Gly Phe Thr Leu Ala Ala Glu Val Tyr
55 60 65tac atg cag gtc gac tac cta caa acc ttt tac cca aat att aaa agc 355Tyr Met Gln Val Asp Tyr Leu Gln Thr Phe Tyr Pro Asn Ile Lys Ser70 75 80 85gtg ctt atc gac gcc cgc ggc cac ggc gcc acc ggc cag atc cgc cca 403Val Leu Ile Asp Ala Arg Gly His Gly Ala Thr Gly Gln Ile Arg Pro
90 95 100gag ctc tgc acc atc gaa gga aca gcg aac gat gtt ctc gca gcc atc 451Glu Leu Cys Thr Ile Glu Gly Thr Ala Asn Asp Val Leu Ala Ala Ile
105 110 115cac gaa cac gca ccg acc ggc ccg ctc att ttg gtt ggg cat tcc ctc 499His Glu His Ala Pro Thr Gly Pro Leu Ile Leu Val Gly His Ser Leu
120 125 130ggc gga ctc acg gca ctt aac ctg gtt aaa cgg gca gat cac tca ctt 547Gly Gly Leu Thr Ala Leu Asn Leu Val Lys Arg Ala Asp His Ser Leu
135 140 145cgg aag agg atc gtc ggc atg gtt cta gtc gcc aca tcg atc gaa tca 595Arg Lys Arg Ile Val Gly Met Val Leu Val Ala Thr Ser Ile Glu Ser150 155 160 165tta tcc acc caa ggt cta cca caa gtc ctg gca tca ccc ctt gcc gac 643Leu Ser Thr Gln Gly Leu Pro Gln Val Leu Ala Ser Pro Leu Ala Asp
170 175 180aac atc aaa aac gcc gtc gaa gca gcc ccc aac gat gcc caa aaa ttc 691Asn Ile Lys Asn Ala Val Glu Ala Ala Pro Asn Asp Ala Gln Lys Phe
185 190 195cgc caa tac gcc acc aca ttt cta gcc ccc acc ctg gcc acc gca gtc 739Arg Gln Tyr Ala Thr Thr Phe Leu Ala Pro Thr Leu Ala Thr Ala Val
200 205 210ttc caa cga gac aca aac gat gaa gtc atc gat ttc cac gcc gcc atg 787Phe Gln Arg Asp Thr Asn Asp Glu Val Ile Asp Phe His Ala Ala Met
215 220 225atc cac gaa acc ccc ttg gat acc ttc gtc ggt ttc ttc gac gac ctc 835Ile His Glu Thr Pro Leu Asp Thr Phe Val Gly Phe Phe Asp Asp Leu230 235 240 245caa gaa cac gac gaa ctc gat gcc gca cca gca ttg gaa ggc ctc aaa 883Gln Glu His Asp Glu Leu Asp Ala Ala Pro Ala Leu Glu Gly Leu Lys
250 255 260ggc tac gtc ctt gcc ggc gaa tta gat gat gtc acc cca att agc caa 931Gly Tyr Val Leu Ala Gly Glu Leu Asp Asp Val Thr Pro Ile Ser Gln
265 270 275gcc gac cgc atc tgc gaa gtc tgg ccc ggc gca cgc ctt caa atc gca 979Ala Asp Arg Ile Cys Glu Val Trp Pro Gly Ala Arg Leu Gln Ile Ala
280 285 290gaa gga gca ggt cat atg ctt ccg ctt gaa gcg cca gga atc ctc aat 1027Glu Gly Ala Gly His Met Leu Pro Leu Glu Ala Pro Gly Ile Leu Asn
295 300 305aat gcg atc ggc aac att ttg gac ggg ctg ggc tgaggaacct ggttcgggcg 1080Asn Ala Ile Gly Asn Ile Leu Asp Gly Leu Gly310 315 320tgg1083<210>260<211>320<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>260Met Thr Pro Asn Gly Arg Arg Gln Leu Leu Leu Glu Arg Gly Ala Ala1 5 10 15Phe Ser Lys Asn Arg Thr Pro Gly Leu Lys His Val Asp Arg His Thr
20 25 30Ile Val Asp Ser Asp Gly Leu Ser Ile His Thr Tyr Met Val Gly His
35 40 45Ala Glu Asn Ala Thr Ala Thr Val Val Phe Ile His Gly Phe Thr Leu
50 55 60Ala Ala Glu Val Tyr Tyr Met Gln Val Asp Tyr Leu Gln Thr Phe Tyr65 70 75 80Pro Asn Ile Lys Ser Val Leu Ile Asp Ala Arg Gly His Gly Ala Thr
85 90 95Gly Gln Ile Arg Pro Glu Leu Cys Thr Ile Glu Gly Thr Ala Asn Asp
100 105 110Val Leu Ala Ala Ile His Glu His Ala Pro Thr Gly Pro Leu Ile Leu
115 120 125Val Gly His Ser Leu Gly Gly Leu Thr Ala Leu Asn Leu Val Lys Arg
130 135 140Ala Asp His Ser Leu Arg Lys Arg Ile Val Gly Met Val Leu Val Ala145 150 155 160Thr Ser Ile Glu Ser Leu Ser Thr Gln Gly Leu Pro Gln Val Leu Ala
165 170 175Ser Pro Leu Ala Asp Asn Ile Lys Asn Ala Val Glu Ala Ala Pro Asn
180 185 190Asp Ala Gln Lys Phe Arg Gln Tyr Ala Thr Thr Phe Leu Ala Pro Thr
195 200 205Leu Ala Thr Ala Val Phe Gln Arg Asp Thr Asn Asp Glu Val Ile Asp
210 215 220Phe His Ala Ala Met Ile His Glu Thr Pro Leu Asp Thr Phe Val Gly225 230 235 240Phe Phe Asp Asp Leu Gln Glu His Asp Glu Leu Asp Ala Ala Pro Ala
245 250 255Leu Glu Gly Leu Lys Gly Tyr Val Leu Ala Gly Glu Leu Asp Asp Val
260 265 270Thr Pro Ile Ser Gln Ala Asp Arg Ile Cys Glu Val Trp Pro Gly Ala
275 280 285Arg Leu Gln Ile Ala Glu Gly Ala Gly His Met Leu Pro Leu Glu Ala
290 295 300Pro Gly Ile Leu Asn Asn Ala Ile Gly Asn Ile Leu Asp Gly Leu Gly305 310 315 320<210>261<211>1137<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1114)<223>RXN01145<400>261taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat ttacagtggg gggcagacat 60cttttcacca aaatttttac gaaaggcgag attttctccc atg gct att gaa ctg 115
Met Ala Ile Glu Leu
1 5ctt tat gat gct gac gct gac ctc tcc ttg atc cag ggc cgt aag gtt 163Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile Gln Gly Arg Lys Val
10 15 20gcc atc gtt ggc tac ggc tcc cag ggc cac gca cac tcc cag aac ctc 211Ala Ile Val Gly Tyr Gly Ser Gln Gly His Ala His Ser Gln Asn Leu
25 30 35cgc gat tct ggc gtt gag gtt gtc att ggt ctg cgc gag ggc tcc aag 259Arg Asp Ser Gly Val Glu Val Val Ile Gly Leu Arg Glu Gly Ser Lys
40 45 50tcc gca gag aag gca aag gaa gca ggc ttc gag gtc aag acc acc gct 307Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu Val Lys Thr Thr Ala
55 60 65gag gct gca gct tgg gct gac gtc atc atg ctc ctg gct cca gac acc 355Glu Ala Ala Ala Trp Ala Asp Val Ile Met Leu Leu Ala Pro Asp Thr70 75 80 85tcc cag gca gaa atc ttc acc aac gac atc gag cca aac ctg aac gca 403Ser Gln Ala Glu Ile Phe Thr Asn Asp Ile Glu Pro Asn Leu Asn Ala
90 95 100ggc gac gca ctg ctg ttc ggc cac ggc ctg aac att cac ttc gac ctg 451Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn Ile His Phe Asp Leu
105 110 115atc aag cca gct gac gac atc atc gtt ggc atg gtt gcg cca aag ggc 499Ile Lys Pro Ala Asp Asp Ile Ile Val Gly Met Val Ala Pro Lys Gly
120 125 130cca ggc cac ttg gtt cgc cgt cag ttc gtt gat ggc aag ggt gtt cct 547Pro Gly His Leu Val Arg Arg Gln Phe Val Asp Gly Lys Gly Val Pro
135 140 145tgc ctc atc gca gtc gac cag gac cca acc gga acc gca cag gct ctg 595Cys Leu Ile Ala Val Asp Gln Asp Pro Thr Gly Thr Ala Gln Ala Leu150 155 160 165acc ctg tcc tac gca gca gca atc ggt ggc gca cgc gca ggc gtt atc 643Thr Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala Arg Ala Gly Val Ile
170 175 180cca acc acc ttc gaa gct gag acc gtc acc gac ctc ttc ggc gag cag 691Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp Leu Phe Gly Glu Gln
185 190 195gct gtt ctc tgc ggt ggc acc gag gaa ctg gtc aag gtt ggc ttc gag 739Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val Lys Val Gly Phe Glu
200 205 210gtt ctc acc gaa gct ggc tac gag cca gag atg gca tac ttc gag gtt 787Val Leu Thr Glu Ala Gly Tyr Glu Pro Glu Met Ala Tyr Phe Glu Val
215 220 225ctt cac gag ctc aag ctc atc gtt gac ctc atg ttc gaa ggt ggc atc 835Leu His Glu Leu Lys Leu Ile Val Asp Leu Met Phe Glu Gly Gly Ile230 235 240 245agc aac atg aac tac tct gtt tct gac acc gct gag ttc ggt ggc tac 883Ser Asn Met Asn Tyr Ser Val Ser Asp Thr Ala Glu Phe Gly Gly Tyr
250 255 260ctc tcc ggc cca cgc gtc atc gat gca gac acc aag tcc cgc atg aag 931Leu Ser Gly Pro Arg Val Ile Asp Ala Asp Thr Lys Ser Arg Met Lys
265 270 275gac atc ctg acc gat atc cag gac ggc acc ttc acc aag cgc ctc atc 979Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe Thr Lys Arg Leu Ile
280 285 290gca aac gtt gag aac ggc aac acc gag ctt gag ggc ctt cgt gct tcc 1027Ala Asn Val Glu Asn Gly Asn Thr Glu Leu Glu Gly Leu Arg Ala Ser
295 300 305tac aac aac cac cca atc gag gag acc ggc gct aag ctc cgc gac ctc 1075Tyr Asn Asn His Pro Ile Glu Glu Thr Gly Ala Lys Leu Arg Asp Leu310 315 320 325atg agc tgg gtc aag gtt gac gct cgc gca gaa acc gct taagtttcac 1124Met Ser Trp Val Lys Val Asp Ala Arg Ala Glu Thr Ala
330 335ccctttgacg gct 1137<210>262<211>338<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>262Met Ala Ile Glu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile1 5 10 15Gln Gly Arg Lys Val Ala Ile Val Gly Tyr Gly Ser Gln Gly His Ala
20 25 30His Ser Gln Asn Leu Arg Asp Ser Gly Val Glu Val Val Ile Gly Leu
35 40 45Arg Glu Gly Ser Lys Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu
50 55 60Val Lys Thr Thr Ala Glu Ala Ala Ala Trp Ala Asp Val Ile Met Leu65 70 75 80Leu Ala Pro Asp Thr Ser Gln Ala Glu Ile Phe Thr Asn Asp Ile Glu
85 90 95Pro Asn Leu Asn Ala Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn
100 105 110Ile His Phe Asp Leu Ile Lys Pro Ala Asp Asp Ile Ile Val Gly Met
115 120 125Val Ala Pro Lys Gly Pro Gly His Leu Val Arg Arg Gln Phe Val Asp
130 135 140Gly Lys Gly Val Pro Cys Leu Ile Ala Val Asp Gln Asp Pro Thr Gly145 150 155 160Thr Ala Gln Ala Leu Thr Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala
165 170 175Arg Ala Gly Val Ile Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp
180 185 190Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val
195 200 205Lys Val Gly Phe Glu Val Leu Thr Glu Ala Gly Tyr Glu Pro Glu Met
210 215 220Ala Tyr Phe Glu Val Leu His Glu Leu Lys Leu Ile Val Asp Leu Met225 230 235 240Phe Glu Gly Gly Ile Ser Asn Met Asn Tyr Ser Val Ser Asp Thr Ala
245 250 255Glu Phe Gly Gly Tyr Leu Ser Gly Pro Arg Val Ile Asp Ala Asp Thr
260 265 270Lys Ser Arg Met Lys Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe
275 280 285Thr Lys Arg Leu Ile Ala Asn Val Glu Ash Gly Asn Thr Glu Leu Glu
290 295 300Gly Leu Arg Ala Ser Tyr Asn Asn His Pro Ile Glu Glu Thr Gly Ala305 310 315 320Lys Leu Arg Asp Leu Met Ser Trp Val Lys Val Asp Ala Arg Ala Glu
325 330 335Thr Ala<210>263<211>487<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(487)<223>RXN03088<400>263tcatggcacc gggacctggc atcgtagcta aggtttcttt tgatgatttc tccgacgtca 60ccggcggcga tgaactcctc gaattggagg caaagaacta atg ggt caa acc cgc 115
Met Gly Gln Thr Arg
1 5atc att tcc ggc gac gca cgc ggc cgc aag atc gaa gta cca cca gca 163Ile Ile Ser Gly Asp Ala Arg Gly Arg Lys Ile Glu Val Pro Pro Ala
10 15 20ggt acc cgc ccc acc tct gac cgc gca cgc gaa ggt ctc ttc tcc tca 211Gly Thr Arg Pro Thr Ser Asp Arg Ala Arg Glu Gly Leu Phe Ser Ser
25 30 35ctg cag gtc cgt ttc gga ttt gag ggc cag cgc gtc ctc gac att ttt 259Leu Gln Val Arg Phe Gly Phe Glu Gly Gln Arg Val Leu Asp Ile Phe
40 45 50gcc ggc tcc ggc gca ctc gga ttg gaa gct gcc tcc agg ggt gcc gat 307Ala Gly Ser Gly Ala Leu Gly Leu Glu Ala Ala Ser Arg Gly Ala Asp
55 60 65gag gta gtt ctg gtc gag tcg aat cct aag gcc gta gag gta att cga 355Glu Val Val Leu Val Glu Ser Asn Pro Lys Ala Val Glu Val Ile Arg70 75 80 85cgg aat gtg gac gtc gta aag cat cct cgc gta acc gtc gca gag atg 403Arg Asn Val Asp Val Val Lys His Pro Arg Val Thr Val Ala Glu Met
90 95 100aaa gca tcc acc tac ctt gcg tcc gca ccc gat aag ttt ttc acg atg 451Lys Ala Ser Thr Tyr Leu Ala Ser Ala Pro Asp Lys Phe Phe Thr Met
105 110 115gtg ctc gcc gac ccg ccc tat gag ctt gcg acg acg 487Val Leu Ala Asp Pro Pro Tyr Glu Leu Ala Thr Thr
120 125<210>264<211>129<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>264Met Gly Gln Thr Arg Ile Ile Ser Gly Asp Ala Arg Gly Arg Lys Ile1 5 10 15Glu Val Pro Pro Ala Gly Thr Arg Pro Thr Ser Asp Arg Ala Arg Glu
20 25 30Gly Leu Phe Ser Ser Leu Gln Val Arg Phe Gly Phe Glu Gly Gln Arg
35 40 45Val Leu Asp Ile Phe Ala Gly Ser Gly Ala Leu Gly Leu Glu Ala Ala
50 55 60Ser Arg Gly Ala Asp Glu Val Val Leu Val Glu Ser Asn Pro Lys Ala65 70 75 80Val Glu Val Ile Arg Arg Asn Val Asp Val Val Lys His Pro Arg Val
85 90 95Thr Val Ala Glu Met Lys Ala Ser Thr Tyr Leu Ala Ser Ala Pro Asp
100 105 110Lys Phe Phe Thr Met Val Leu Ala Asp Pro Pro Tyr Glu Leu Ala Thr
115 120 125Thr<210>265<211>639<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(616)<223> RXN02952<400>265tccaaaccgc tgtttctgct cgtggcgaat cctggatatt gaagtaattg aaatccgaga 60cctgatcttt gatctcgcta cctcattcac aagcgccggc atg agc tcc cca gca 115
Met Ser Ser Pro Ala
1 5ctt gac gct gca aaa cag cgc ctt gct gaa tcc gat ggc ctg atc gct 163Leu Asp Ala Ala Lys Gln Arg Leu Ala Glu Ser Asp Gly Leu Ile Ala
10 15 20gtt acc cca gta ttt acc gcg agc tac tcc ggc atc ttc aag atg ttc 211Val Thr Pro Val Phe Thr Ala Ser Tyr Ser Gly Ile Phe Lys Met Phe
25 30 35ttt gat gtc ctg gac ccc aag acc att gtg ggt ctg ccc acc atc att 259Phe Asp Val Leu Asp Pro Lys Thr Ile Val Gly Leu Pro Thr Ile Ile
40 45 50gcg gca tct gct gga acg gca cgc cac tca ttg gtt ctc gac cac gcc 307Ala Ala Ser Ala Gly Thr Ala Arg His Ser Leu Val Leu Asp His Ala
55 60 65atc cga cca ctg ttt acc tac ttg cga gca gtt gtc gta ccc acc ggc 355Ile Arg Pro Leu Phe Thr Tyr Leu Arg Ala Val Val Val Pro Thr Gly70 75 80 85gtg ttc gca gcc acg gaa gat ttc ggc act gaa gct ggc gca gac att 403Val Phe Ala Ala Thr Glu Asp Phe Gly Thr Glu Ala Gly Ala Asp Ile
90 95 100gaa cgt cgc gtg aac cgc gca gct ggc gaa tta gcg aca ctc atg ttg 451Glu Arg Arg Val Asn Arg Ala Ala Gly Glu Leu Ala Thr Leu Met Leu
105 110 115cag gat tac tcc agt gtg caa ggc ctt ggg ggc gca acc gcg aac caa 499Gln Asp Tyr Ser Ser Val Gln Gly Leu Gly Gly Ala Thr Ala Asn Gln
120 125 130gac gct gac ctt tcc ttc cgt cgc acc act ggc gtg acc ccg gga gag 547Asp Ala Asp Leu Ser Phe Arg Arg Thr Thr Gly Val Thr Pro Gly Glu
135 140 145aac ttc agc agc ttt gcc gat ctt tct caa agg aca cga cgg aaa cgg 595Asn Phe Ser Ser Phe Ala Asp Leu Ser Gln Arg Thr Arg Arg Lys Arg150 155 160 165cta aat tcg cgg atc tcc gtt taaggcattg aagcatttgg agg 639Leu Asn Ser Arg Ile Ser Val
170<210>266<211>172<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>266Met Ser Ser Pro Ala Leu Asp Ala Ala Lys Gln Arg Leu Ala Glu Ser1 5 10 15Asp Gly Leu Ile Ala Val Thr Pro Val Phe Thr Ala Ser Tyr Ser Gly
20 25 30Ile Phe Lys Met Phe Phe Asp Val Leu Asp Pro Lys Thr Ile Val Gly
35 40 45Leu Pro Thr Ile Ile Ala Ala Ser Ala Gly Thr Ala Arg His Ser Leu
50 55 60Val Leu Asp His Ala Ile Arg Pro Leu Phe Thr Tyr Leu Arg Ala Val65 70 75 80Val Val Pro Thr Gly Val Phe Ala Ala Thr Glu Asp Phe Gly Thr Glu
85 90 95Ala Gly Ala Asp Ile Glu Arg Arg Val Asn Arg Ala Ala Gly Glu Leu
100 105 110Ala Thr Leu Met Leu Gln Asp Tyr Ser Ser Val Gln Gly Leu Gly Gly
115 120 125Ala Thr Ala Asn Gln Asp Ala Asp Leu Ser Phe Arg Arg Thr Thr Gly
130 135 140Val Thr Pro Gly Glu Asn Phe Ser Ser Phe Ala Asp Leu Ser Gln Arg145 150 155 160Thr Arg Arg Lys Arg Leu Asn Ser Arg Ile Ser Val
165 170<210>267<211>1044<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1021)<223>RXN00513<400>267cacagcgtac gcacgagctt gaggatcttt cagaactcaa cattgaattg gatgccgata 60ttttggccaa ggctcctgtg attccggaag gactgttctg atg gcg ggt ttg ttt 115
Met Ala Gly Leu Phe
1 5tcc tct gct gtt gca cca acg gag cgt cga aaa gca tta cgc gcg gca 163Ser Ser Ala Val Ala Pro Thr Glu Arg Arg Lys Ala Leu Arg Ala Ala
10 15 20ctg gct gcg cct gaa att gcc cgc atg cct ggt gca ttc tcc ccg ctg 211Leu Ala Ala Pro Glu Ile Ala Arg Met Pro Gly Ala Phe Ser Pro Leu
25 30 35gcg gcg cgc gca atc cag gaa gcc gga ttt gaa ggc gtg tac gtc tcg 259Ala Ala Arg Ala Ile Gln Glu Ala Gly Phe Glu Gly Val Tyr Val Ser
40 45 50ggc gcc gtc gtg gcg gct gac ctt gca ttg ccg gat atc ggc ttg acc 307Gly Ala Val Val Ala Ala Asp Leu Ala Leu Pro Asp Ile Gly Leu Thr
55 60 65aca ttg acc gaa gtg gcg cac cgc tcc cgg cag atc gca cgc gtg aca 355Thr Leu Thr Glu Val Ala His Arg Ser Arg Gln Ile Ala Arg Val Thr70 75 80 85gac ttg ccc gtg ctg gtc gac gcc gac acc ggc ttc ggc gaa ccc atg 403Asp Leu Pro Val Leu Val Asp Ala Asp Thr Gly Phe Gly Glu Pro Met
90 95 100tcc gca gcg cgc acc gtc tcc gaa ctc gaa gat gca ggt gtc gcg ggc 451Ser Ala Ala Arg Thr Val Ser Glu Leu Glu Asp Ala Gly Val Ala Gly
105 110 115tgc cac ctg gaa gat caa gtc aac ccc aaa cgc tgt ggg cac ctg gac 499Cys His Leu Glu Asp Gln Val Asn Pro Lys Arg Cys Gly His Leu Asp
120 125 130gga aaa gaa gta gtg ggc acg gac atc atg gtt cgt cgc atc gcc gca 547Gly Lys Glu Val Val Gly Thr Asp Ile Met Val Arg Arg Ile Ala Ala
135 140 145gct gtc aac gag cgt cgc gat gag caa ttc gtc atc tgc gct cgc acc 595Ala Val Asn Glu Arg Arg Asp Glu Gln Phe Val Ile Cys Ala Arg Thr150 155 160 165gac gcc gcg gga gtg gaa ggc atc gac tcc gcg atc gag cgc gcc aaa 643Asp Ala Ala Gly Val Glu Gly Ile Asp Ser Ala Ile Glu Arg Ala Lys
170 175 180gct tac gcg gat gcc ggc gcc gac atg atc ttc acc gaa gcg ctg tac 691Ala Tyr Ala Asp Ala Gly Ala Asp Met Ile Phe Thr Glu Ala Leu Tyr
185 190 195agc cct gca gat ttt gaa aaa ttc cgc gcg gcc gtc gac att ccg ctg 739Ser Pro Ala Asp Phe Glu Lys Phe Arg Ala Ala Val Asp Ile Pro Leu
200 205 210ctg gcc aac atg acg gaa ttt ggc aaa acc gaa ctt ctg ccc gcg cag 787Leu Ala Asn Met Thr Glu Phe Gly Lys Thr Glu Leu Leu Pro Ala Gln
215 220 225ctt ctg gaa gac atc gga tac aac gca gtg atc tac cca gtg acc ctg 835Leu Leu Glu Asp Ile Gly Tyr Asn Ala Val Ile Tyr Pro Val Thr Leu230 235 240 245ctg cgc att gcg atg gga cag gtc gaa caa gct ctc ggc gac att gca 883Leu Arg Ile Ala Met Gly Gln Val Glu Gln Ala Leu Gly Asp Ile Ala
250 255 260aac acc gga atc caa acc gac tgg gtc gac cgg atg caa cac cga tcc 931Asn Thr Gly Ile Gln Thr Asp Trp Val Asp Arg Met Gln His Arg Ser
265 270 275agg ctg tat gag ctg ctg cgc tac aac gag tac aac gct ttc gac cag 979Arg Leu Tyr Glu Leu Leu Arg Tyr Asn Glu Tyr Asn Ala Phe Asp Gln
280 285 290caa gta ttc acc tat tcc gct gac agc tac aag ccc atc ttc 1021Gln Val Phe Thr Tyr Ser Ala Asp Ser Tyr Lys Pro Ile Phe
295 300 305taacccgcct atatataagg agt 1044<210>268<211>307<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>268Met Ala Gly Leu Phe Ser Ser Ala Val Ala Pro Thr Glu Arg Arg Lys1 5 10 15Ala Leu Arg Ala Ala Leu Ala Ala Pro Glu Ile Ala Arg Met Pro Gly
20 25 30Ala Phe Ser Pro Leu Ala Ala Arg Ala Ile Gln Glu Ala Gly Phe Glu
35 40 45Gly Val Tyr Val Ser Gly Ala Val Val Ala Ala Asp Leu Ala Leu Pro
50 55 60Asp Ile Gly Leu Thr Thr Leu Thr Glu Val Ala His Arg Ser Arg Gln65 70 75 80Ile Ala Arg Val Thr Asp Leu Pro Val Leu Val Asp Ala Asp Thr Gly
85 90 95Phe Gly Glu Pro Met Ser Ala Ala Arg Thr Val Ser Glu Leu Glu Asp
100 105 110Ala Gly Val Ala Gly Cys His Leu Glu Asp Gln Val Asn Pro Lys Arg
115 120 125Cys Gly His Leu Asp Gly Lys Glu Val Val Gly Thr Asp Ile Met Val
130 135 l40Arg Arg Ile Ala Ala Ala Val Asn Glu Arg Arg Asp Glu Gln Phe Val145 150 155 160Ile Cys Ala Arg Thr Asp Ala Ala Gly Val Glu Gly Ile Asp Ser Ala
165 170 175Ile Glu Arg Ala Lys Ala Tyr Ala Asp Ala Gly Ala Asp Met Ile Phe
180 185 190Thr Glu Ala Leu Tyr Ser Pro Ala Asp Phe Glu Lys Phe Arg Ala Ala
195 200 205Val Asp Ile Pro Leu Leu Ala Asn Met Thr Glu Phe Gly Lys Thr Glu
210 215 220Leu Leu Pro Ala Gln Leu Leu Glu Asp Ile Gly Tyr Asn Ala Val Ile225 230 235 240Tyr Pro Val Thr Leu Leu Arg Ile Ala Met Gly Gln Val Glu Gln Ala
245 250 255Leu Gly Asp Ile Ala Asn Thr Gly Ile Gln Thr Asp Trp Val Asp Arg
260 265 270Met Gln His Arg Ser Arg Leu Tyr Glu Leu Leu Arg Tyr Asn Glu Tyr
275 280 285Asn Ala Phe Asp Gln Gln Val Phe Thr Tyr Ser Ala Asp Ser Tyr Lys
290 295 300Pro Ile Phe305<210>269<211>957<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(934)<223>RXN01152<400>269agtgaaggat ctgatgtggt ttgaacaagc cctggaagcc tatctggtaa attaacgccg 60agttcaatca agacaagcac acagaagaaa gtgagggctc atg ccc tac tca ggt 115
Met Pro Tyr Ser Gly
1 5ccg ttc caa gca ggc gac cgc gtt cag ctc acc gac gct aaa cgc cgc 163Pro Phe Gln Ala Gly Asp Arg Val Gln Leu Thr Asp Ala Lys Arg Arg
10 15 20cat ttc acc atc att ttg gaa cca gga acc acc tac cac acc cac cgt 211His Phe Thr Ile Ile Leu Glu Pro Gly Thr Thr Tyr His Thr His Arg
25 30 35gga caa atc gca cac gat gac atc atc ggc gcc gat gag ggc act gtt 259Gly Gln Ile Ala His Asp Asp Ile Ile Gly Ala Asp Glu Gly Thr Val
40 45 50gtc cac tcc acc atg ggc tct gat tac ttg tgc ttc cgt cac ctc atg 307Val His Ser Thr Met Gly Ser Asp Tyr Leu Cys Phe Arg His Leu Met
55 60 65gtt gat cac gtg ctg agc atg cct cgt ggc gct gca gtt att tat cca 355Val Asp His Val Leu Ser Met Pro Arg Gly Ala Ala Val Ile Tyr Pro70 75 80 85aag gac tct gca cag att ctg gtc gag ggc gat att ttc cct ggc gcc 403Lys Asp Ser Ala Gln Ile Leu Val Glu Gly Asp Ile Phe Pro Gly Ala
90 95 100cga gtt ctg gaa gct ggc gct ggt tcc ggt gca ctg tcc atg gcg ctg 451Arg Val Leu Glu Ala Gly Ala Gly Ser Gly Ala Leu Ser Met Ala Leu
105 110 115ctt cgt gca gtg ggt gaa aag ggc aat gtc atc tcc tac gaa atc cgt 499Leu Arg Ala Val Gly Glu Lys Gly Asn Val Ile Ser Tyr Glu Ile Arg
120 125 130gag gat cac ctg gag tac gca gtc tcc aac gtg gag gag tac ttc ggt 547Glu Asp His Leu Glu Tyr Ala Val Ser Asn Val Glu Glu Tyr Phe Gly
135 140 145gag cgt cca gca acc tgg gat cca cgt ctt ggt gac ctg aaa gaa gtc 595Glu Arg Pro Ala Thr Trp Asp Pro Arg Leu Gly Asp Leu Lys Glu Val150 155 160 165acc gtt gag gat ctc ggc gga cct gtt gac cgc atc atc ttg gat atg 643Thr Val Glu Asp Leu Gly Gly Pro Val Asp Arg Ile Ile Leu Asp Met
170 175 180ctt gag ccg tgg gaa atg ctg gag acc tgc aag gat ctt ctc atc cct 691Leu Glu Pro Trp Glu Met Leu Glu Thr Cys Lys Asp Leu Leu Ile Pro
185 190 195ggt ggt gtg ttt atg acg tat gtg gcg acc gtg cca cag ctg atg aag 739Gly Gly Val Phe Met Thr Tyr Val Ala Thr Val Pro Gln Leu Met Lys
200 205 210gtc atg gaa ggc atc cgc gag caa aaa tgc ttc acg gag cca cgc gcg 787Val Met Glu Gly Ile Arg Glu Gln Lys Cys Phe Thr Glu Pro Arg Ala
215 220 225tgg gaa tct ttg gtt cgt gat tgg aag gtg gag ggc ttg gca aca cgc 835Trp Glu Ser Leu Val Arg Asp Trp Lys Val Glu Gly Leu Ala Thr Arg230 235 240 245cct gag cac cgc atg aat gcc cac acc gcg ttc ttg gtg ttg acc agg 883Pro Glu His Arg Met Asn Ala His Thr Ala Phe Leu Val Leu Thr Arg
250 255 260cgt ttg gct gat ggc gtg gag cct cct cgt ccg cag cgt aag gca cgt 931Arg Leu Ala Asp Gly Val Glu Pro Pro Arg Pro Gln Arg Lys Ala Arg
265 270 275cga taaaaagacc tagttggagg gcg 957Arg<210>270<211>278<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>270Met Pro Tyr Ser Gly Pro Phe Gln Ala Gly Asp Arg Val Gln Leu Thr1 5 10 15Asp Ala Lys Arg Arg His Phe Thr Ile Ile Leu Glu Pro Gly Thr Thr
20 25 30Tyr His Thr His Arg Gly Gln Ile Ala His Asp Asp Ile Ile Gly Ala
35 40 45Asp Glu Gly Thr Val Val His Ser Thr Met Gly Ser Asp Tyr Leu Cys
50 55 60Phe Arg His Leu Met Val Asp His Val Leu Ser Met Pro Arg Gly Ala65 70 75 80Ala Val Ile Tyr Pro Lys Asp Ser Ala Gln Ile Leu Val Glu Gly Asp
85 90 95Ile Phe Pro Gly Ala Arg Val Leu Glu Ala Gly Ala Gly Ser Gly Ala
100 105 110Leu Ser Met Ala Leu Leu Arg Ala Val Gly Glu Lys Gly Asn Val Ile
115 120 125Ser Tyr Glu Ile Arg Glu Asp His Leu Glu Tyr Ala Val Ser Asn Val
130 135 140Glu Glu Tyr Phe Gly Glu Arg Pro Ala Thr Trp Asp Pro Arg Leu Gly145 150 155 160Asp Leu Lys Glu Val Thr Val Glu Asp Leu Gly Gly Pro Val Asp Arg
165 170 175Ile Ile Leu Asp Met Leu Glu Pro Trp Glu Met Leu Glu Thr Cys Lys
180 185 190Asp Leu Leu Ile Pro Gly Gly Val Phe Met Thr Tyr Val Ala Thr Val
195 200 205Pro Gln Leu Met Lys Val Met Glu Gly Ile Arg Glu Gln Lys Cys Phe
210 215 220Thr Glu Pro Arg Ala Trp Glu Ser Leu Val Arg Asp Trp Lys Val Glu225 230 235 240Gly Leu Ala Thr Arg Pro Glu His Arg Met Asn Ala His Thr Ala Phe
245 250 255Leu Val Leu Thr Arg Arg Leu Ala Asp Gly Val Glu Pro Pro Arg Pro
260 265 270Gln Arg Lys Ala Arg Arg
275<210>271<211>2025<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(2002)<223>RXN00787<400>271ccagcccgcc caataaataa tttctctctt ctaattgcgg agcctcatat attgagtacg 60gtattttgaa acaccttcag cccccttttt aggagccaca gtg tct cag cct ctc 115
Val Ser Gln Pro Leu
1 5agc aag cgt ctc agc ata cga aaa gca ctc gcc agc gcc ttc ata gtt 163Ser Lys Arg Leu Ser Ile Arg Lys Ala Leu Ala Ser Ala Phe Ile Val
10 15 20gcg ctg gcg ttt tcg ctt tcc cca gta gcc aaa gcc caa gcc aat gaa 211Ala Leu Ala Phe Ser Leu Ser Pro Val Ala Lys Ala Gln Ala Asn Glu
25 30 35act ccg acg atg atc gtg ttg gac aat tca ggc tcc atg aca gct caa 259Thr Pro Thr Met Ile Val Leu Asp Asn Ser Gly Ser Met Thr Ala Gln
40 45 50gat gcc ggc gga cag acc cgt atc gat gca gca aaa caa gcc tcc act 307Asp Ala Gly Gly Gln Thr Arg Ile Asp Ala Ala Lys Gln Ala Ser Thr
55 60 65cag tta att aat gac atc tcc gac cgc acc gac gta ggt ctg acc tac 355Gln Leu Ile Asn Asp Ile Ser Asp Arg Thr Asp Val Gly Leu Thr Tyr70 75 80 85tac ggc gga aac acc ggc gaa aca gaa gca gac gtt gag atg gga tgc 403Tyr Gly Gly Asn Thr Gly Glu Thr Glu Ala Asp Val Glu Met Gly Cys
90 95 100caa gac gtc acc atc ctt ggc ggc ccc tcc cga gga aat gca gac acc 451Gln Asp Val Thr Ile Leu Gly Gly Pro Ser Arg Gly Asn Ala Asp Thr
105 110 115tta att gac acg atc aac agc ctg cag cct cga ggc ttc acc ccc atc 499Leu Ile Asp Thr Ile Asn Ser Leu Gln Pro Arg Gly Phe Thr Pro Ile
120 125 130ggc aaa gca ctc acc gat acc gcc gcc gag ctc ccc gaa ggc gga aac 547Gly Lys Ala Leu Thr Asp Thr Ala Ala Glu Leu Pro Glu Gly Gly Asn
135 140 145att gtg ttg gtc tcc gat ggc atc gcc aac tgc acc cca ccg gat gtc 595Ile Val Leu Val Ser Asp Gly Ile Ala Asn Cys Thr Pro Pro Asp Val150 155 160 165tgc gaa gta gcc caa gaa ctg gct caa agt gga atc aac ctg gtt atc 643Cys Glu Val Ala Gln Glu Leu Ala Gln Ser Gly Ile Asn Leu Val Ile
170 175 180aac acc atc gga cta aat gtt gat cca gca gcg cgc gaa gaa ctg gag 691Asn Thr Ile Gly Leu Asn Val Asp Pro Ala Ala Arg Glu Glu Leu Glu
185 190 195tgc atc gct gga gtc ggt ggt ggc act tac gcg gat gct tcc gac gcg 739Cys Ile Ala Gly Val Gly Gly Gly Thr Tyr Ala Asp Ala Ser Asp Ala
200 205 210cag agc ctt acc gat gcg ctg aca cga gcc gcc agt agg caa tac aac 787Gln Ser Leu Thr Asp Ala Leu Thr Arg Ala Ala Ser Arg Gln Tyr Asn
215 220 225tct tac acc tcc gat gtg aca aaa att gat ggg gca tcg gaa caa agc 835Ser Tyr Thr Ser Asp Val Thr Lys Ile Asp Gly Ala Ser Glu Gln Ser230 235 240 245gca gcc gta gaa att gat gag gat aca gaa cta ttc ctc acc gac ctg 883Ala Ala Val Glu Ile Asp Glu Asp Thr Glu Leu Phe Leu Thr Asp Leu
250 255 260cca caa gaa tcc cgc ttt tgg aaa atc cct gta gag cca ggt gaa acc 931Pro Gln Glu Ser Arg Phe Trp Lys Ile Pro Val Glu Pro Gly Glu Thr
265 270 275atc tca gtt tct gcc aac aca gtt acc gac cca aca gta ctc acc atg 979Ile Ser Val Ser Ala Asn Thr Val Thr Asp Pro Thr Val Leu Thr Met
280 285 290ggg caa ggc gga atc aag ctt gaa gcc caa ctc cat act gaa gag gct 1027Gly Gln Gly Gly Ile Lys Leu Glu Ala Gln Leu His Thr Glu Glu Ala
295 300 305cca caa tac ggc ctg cgt ggt cgg tgc act cgg gtc tca ttt gat aat 1075Pro Gln Tyr Gly Leu Arg Gly Arg Cys Thr Arg Val Ser Phe Asp Asn310 315 320 325ttc aag ccc ggc ctt ggt gta cgc gga atc caa aac gcg tcc gtt gca 1123Phe Lys Pro Gly Leu Gly Val Arg Gly Ile Gln Asn Ala Ser Val Ala
330 335 340tca aaa gaa gtg ggc acc aac aac tgt gac acc gat gcc atc tac ctc 1171Ser Lys Glu Val Gly Thr Asn Asn Cys Asp Thr Asp Ala Ile Tyr Leu
345 350 355gaa att tct aga agc gga gat tac ctc aac ggg cag gac att cca acg 1219Glu Ile Ser Arg Ser Gly Asp Tyr Leu Asn Gly Gln Asp Ile Pro Thr
360 365 370gaa atc acc atc gag cgc ttc gga aaa gta gat gaa tca aca atc gga 1267Glu Ile Thr Ile Glu Arg Phe Gly Lys Val Asp Glu Ser Thr Ile Gly
375 380 385aat gtc aca gag gaa cat agc tcc gtc gat ctt acc gag gct gca gca 1315Asn Val Thr Glu Glu His Ser Ser Val Asp Leu Thr Glu Ala Ala Ala390 395 400 405tca gag gca cac cct gtc aca cct ggc cag tgg ttc aca tcg gcc gct 1363Ser Glu Ala His Pro Val Thr Pro Gly Gln Trp Phe Thr Ser Ala Ala
410 415 420gat cta gat ccc gca ggt gag aaa gtc tcc tcc atc atc gtt cca gga 1411Asp Leu Asp Pro Ala Gly Glu Lys Val Ser Ser Ile Ile Val Pro Gly
425 430 435gaa acc cac ttc tat gcg ctg cct gtc gac tac ggc caa gaa ctg cgc 1459Glu Thr His Phe Tyr Ala Leu Pro Val Asp Tyr Gly Gln Glu Leu Arg
440 445 450gca gct gta gaa aca act ttt gac caa atc gac agt tcc gcg ctt ggc 1507Ala Ala Val Glu Thr Thr Phe Asp Gln Ile Asp Ser Ser Ala Leu Gly
455 460 465acg cat ctt tat atc caa gcg ttc agc cca aac cgg gca gag ata gag 1555Thr His Leu Tyr Ile Gln Ala Phe Ser Pro Asn Arg Ala Glu Ile Glu470 475 480 485ctc acc aat aga gat acg tca tat gcg gac gac aac ggg ctc aaa act 1603Leu Thr Asn Arg Asp Thr Ser Tyr Ala Asp Asp Asn Gly Leu Lys Thr
490 495 500ttt gga ttc ttc acc cca gtg agt gca gca aat ttg ttc gag aaa agt 1651Phe Gly Phe Phe Thr Pro Val Ser Ala Ala Asn Leu Phe Glu Lys Ser
505 510 515tct caa ggc ata tcg cta agg agc cca tgg caa ggt ggc acc caa tac 1699Ser Gln Gly Ile Ser Leu Arg Ser Pro Trp Gln Gly Gly Thr Gln Tyr
520 525 530ctc gca gtg aca tac cta cca agt ggt caa gat gaa gat gta tcc gca 1747Leu Ala Val Thr Tyr Leu Pro Ser Gly Gln Asp Glu Asp Val Ser Ala
535 540 545act gat cag ctg ccc aca ttg gaa tat gaa ctc gtg gca gaa gcg ttt 1795Thr Asp Gln Leu Pro Thr Leu Glu Tyr Glu Leu Val Ala Glu Ala Phe550 555 560 565gga gac cct gtt gac cca ccg gtt ttc gct tca ttg acg gga gca acc 1843Gly Asp Pro Val Asp Pro Pro Val Phe Ala Ser Leu Thr Gly Ala Thr
570 575 580cca agc acc tcc acc ccc cca tca gat gtt gcg gaa gat gaa caa atc 1891Pro Ser Thr Ser Thr Pro Pro Ser Asp Val Ala Glu Asp Glu Gln Ile
585 590 595tcc gag gca aca gaa gaa gac tca agc agt ttc ccc atc gtg tgg att 1939Ser Glu Ala Thr Glu Glu Asp Ser Ser Ser Phe Pro Ile Val Trp Ile
600 605 610ggg ctg ggt gtc att ggc tta ggc ata atc att ggt ttg atc ttt gcg 1987Gly Leu Gly Val Ile Gly Leu Gly Ile Ile Ile Gly Leu Ile Phe Ala
615 620 625ctg aga aga aag aat taagccctaa aagataaaga gtc 2025Leu Arg Arg Lys Asn630<210>272<211>634<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>272Val Ser Gln Pro Leu Ser Lys Arg Leu Ser Ile Arg Lys Ala Leu Ala1 5 10 15Ser Ala Phe Ile Val Ala Leu Ala Phe Ser Leu Ser Pro Val Ala Lys
20 25 30Ala Gln Ala Asn Glu Thr Pro Thr Met Ile Val Leu Asp Asn Ser Gly
35 40 45Ser Met Thr Ala Gln Asp Ala Gly Gly Gln Thr Arg Ile Asp Ala Ala
50 55 60Lys Gln Ala Ser Thr Gln Leu Ile Asn Asp Ile Ser Asp Arg Thr Asp65 70 75 80Val Gly Leu Thr Tyr Tyr Gly Gly Asn Thr Gly Glu Thr Glu Ala Asp
85 90 95Val Glu Met Gly Cys Gln Asp Val Thr Ile Leu Gly Gly Pro Ser Arg
100 105 110Gly Asn Ala Asp Thr Leu Ile Asp Thr Ile Asn Ser Leu Gln Pro Arg
115 120 125Gly Phe Thr Pro Ile Gly Lys Ala Leu Thr Asp Thr Ala Ala Glu Leu
130 135 140Pro Glu Gly Gly Asn Ile Val Leu Val Ser Asp Gly Ile Ala Asn Cys145 150 155 160Thr Pro Pro Asp Val Cys Glu Val Ala Gln Glu Leu Ala Gln Ser Gly
165 170 175Ile Asn Leu Val Ile Asn Thr Ile Gly Leu Asn Val Asp Pro Ala Ala
180 185 190Arg Glu Glu Leu Glu Cys Ile Ala Gly Val Gly Gly Gly Thr Tyr Ala
195 200 205Asp Ala Ser Asp Ala Gln Ser Leu Thr Asp Ala Leu Thr Arg Ala Ala
210 215 220Ser Arg Gln Tyr Asn Ser Tyr Thr Ser Asp Val Thr Lys Ile Asp Gly225 230 235 240Ala Ser Glu Gln Ser Ala Ala Val Glu Ile Asp Glu Asp Thr Glu Leu
245 250 255Phe Leu Thr Asp Leu Pro Gln Glu Ser Arg Phe Trp Lys Ile Pro Val
260 265 270Glu Pro Gly Glu Thr Ile Ser Val Ser Ala Asn Thr Val Thr Asp Pro
275 280 285Thr Val Leu Thr Met Gly Gln Gly Gly Ile Lys Leu Glu Ala Gln Leu
290 295 300His Thr Glu Glu Ala Pro Gln Tyr Gly Leu Arg Gly Arg Cys Thr Arg305 310 315 320Val Ser Phe Asp Asn Phe Lys Pro Gly Leu Gly Val Arg Gly Ile Gln
325 330 335Asn Ala Ser Val Ala Ser Lys Glu Val Gly Thr Asn Asn Cys Asp Thr
340 345 350Asp Ala Ile Tyr Leu Glu Ile Ser Arg Ser Gly Asp Tyr Leu Asn Gly
355 360 365Gln Asp Ile Pro Thr Glu Ile Thr Ile Glu Arg Phe Gly Lys Val Asp
370 375 380Glu Ser Thr Ile Gly Asn Val Thr Glu Glu His Ser Ser Val Asp Leu385 390 395 400Thr Glu Ala Ala Ala Ser Glu Ala His Pro Val Thr Pro Gly Gln Trp
405 410 415Phe Thr Ser Ala Ala Asp Leu Asp Pro Ala Gly Glu Lys Val Ser Ser
420 425 430Ile Ile Val Pro Gly Glu Thr His Phe Tyr Ala Leu Pro Val Asp Tyr
435 440 445Gly Gln Glu Leu Arg Ala Ala Val Glu Thr Thr Phe Asp Gln Ile Asp
450 455 460Ser Ser Ala Leu Gly Thr His Leu Tyr Ile Gln Ala Phe Ser Pro Asn465 470 475 480Arg Ala Glu Ile Glu Leu Thr Asn Arg Asp Thr Ser Tyr Ala Asp Asp
485 490 495Asn Gly Leu Lys Thr Phe Gly Phe Phe Thr Pro Val Ser Ala Ala Asn
500 505 510Leu Phe Glu Lys Ser Ser Gln Gly Ile Ser Leu Arg Ser Pro Trp Gln
515 520 525Gly Gly Thr Gln Tyr Leu Ala Val Thr Tyr Leu Pro Ser Gly Gln Asp
530 535 540Glu Asp Val Ser Ala Thr Asp Gln Leu Pro Thr Leu Glu Tyr Glu Leu545 550 555 560Val Ala Glu Ala Phe Gly Asp Pro Val Asp Pro Pro Val Phe Ala Ser
565 570 575Leu Thr Gly Ala Thr Pro Ser Thr Ser Thr Pro Pro Ser Asp Val Ala
580 585 590Glu Asp Glu Gln Ile Ser Glu Ala Thr Glu Glu Asp Ser Ser Ser Phe
595 600 605Pro Ile Val Trp Ile Gly Leu Gly Val Ile Gly Leu Gly Ile Ile Ile
610 615 620Gly Leu Ile Phe Ala Leu Arg Arg Lys Asn625 630<210>273<211>576<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(553)<223>RXN01302<400>273tggggctgcg tggtgttctt catcatcgcc accgctttga cctggatcta ctacgcccgc 60ccgaacgctc cattcccggg ataaaccgaa aggccaatcc atg act aca act act 115
Met Thr Thr Thr Thr
1 5tct tct ggg aag tct tct gaa aag atc aac ccc ctc ttc aag ctc ggc 163Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro Leu Phe Lys Leu Gly
10 15 20agt ttc cta aga aaa ggc acc gtc ggt tct gaa ggc cag cag att ttc 211Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu Gly Gln Gln Ile Phe
25 30 35ctt cag ggc gga cgc caa gcc gat gtg ttt tat cgc aac cga tgg gct 259Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr Arg Asn Arg Trp Ala
40 45 50ttc gat aaa gtc gtg cgc tcc aca cat ggc gtg aac tgc acg ggc tcc 307Phe Asp Lys Val Val Arg Ser Thr His Gly Val Asn Cys Thr Gly Ser
55 60 65tgc tcg tgg aaa gtg tat gta aaa gac ggt gtg atc acc tgg gaa tcc 355Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val Ile Thr Trp Glu Ser70 75 80 85cag gca gtg gat tac cca act acc ggt gcg gat atg ccc gac aat gaa 403Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp Met Pro Asp Asn Glu
90 95 100cca cgt ggc tgc cct cgt ggt gca tca ttt tcc tgg tac acc tac tcc 451Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser Trp Tyr Thr Tyr Ser
105 110 115cca acc cgc atc cgc tac cca tac atc ggt ggc gtg cta gtt gat atg 499Pro Thr Arg Ile Arg Tyr Pro Tyr Ile Gly Gly Val Leu Val Asp Met
120 125 130tcc gcg aag cca agg aac gcc tgg gcg atc cgg tgc tgg cgt ggc gcg 547Ser Ala Lys Pro Arg Asn Ala Trp Ala Ile Arg Cys Trp Arg Gly Ala
135 140 145aca ttg tagaaacccc agaaaagcgc aaa 576Thr Leu150<210>274<211>151<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>274Met Thr Thr Thr Thr Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro1 5 10 15Leu Phe Lys Leu Gly Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu
20 25 30Gly Gln Gln Ile Phe Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr
35 40 45Arg Asn Arg Trp Ala Phe Asp Lys Val Val Arg Ser Thr His Gly Val
50 55 60Asn Cys Thr Gly Ser Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val65 70 75 80Ile Thr Trp Glu Ser Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp
85 90 95Met Pro Asp Asn Glu Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser
100 105 110Trp Tyr Thr Tyr Ser Pro Thr Arg Ile Arg Tyr Pro Tyr Ile Gly Gly
115 120 125Val Leu Val Asp Met Ser Ala Lys Pro Arg Asn Ala Trp Ala Ile Arg
130 135 140Cys Trp Arg Gly Ala Thr Leu145 150<210>275<211>466<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(466)<223>FRXA01302<400>275tggggctgcg tggtgttctt catcatcgcc accgctttga cctggatcta ctacgcccgc 60ccgaacgctc cattcccggg ataaaccgaa aggccaatcc atg act aca act act 115
Met Thr Thr Thr Thr
1 5tct tct ggg aag tct tct gaa aag atc aac ccc ctc ttc aag ctc ggc 163Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro Leu Phe Lys Leu Gly
10 15 20agt ttc cta aga aaa ggc acc gtc ggt tct gaa ggc cag cag att ttc 211Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu Gly Gln Gln Ile Phe
25 30 35ctt cag ggc gga cgc caa gcc gat gtg ttt tat cgc aac cga tgg gct 259Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr Arg Asn Arg Trp Ala
40 45 50ttc gat aaa gtc gtg cgc tcc aca cat ggc gtg aac tgc acg ggc tcc 307Phe Asp Lys Val Val Arg Ser Thr His Gly Val Asn Cys Thr Gly Ser
55 60 65tgc tcg tgg aaa gtg tat gta aaa gac ggt gtg atc acc tgg gaa tcc 355Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val Ile Thr Trp Glu Ser70 75 80 85cag gca gtg gat tac cca act acc ggt gcg gat atg ccc gac aat gaa 403Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp Met Pro Asp Asn Glu
90 95 100cca cgt ggc tgc cct cgt ggt gca tca ttt tcc tgg tac acc tac tcc 451Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser Trp Tyr Thr Tyr Ser
105 110 115cca acc ggc atc cgc 466Pro Thr Gly Ile Arg
120<210>276<211>122<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>276Met Thr Thr Thr Thr Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro1 5 10 15Leu Phe Lys Leu Gly Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu
20 25 30Gly Gln Gln Ile Phe Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr
35 40 45Arg Asn Arg Trp Ala Phe Asp Lys Val Val Arg Ser Thr His Gly Val
50 55 60Asn Cys Thr Gly Ser Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val65 70 75 80Ile Thr Trp Glu Ser Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp
85 90 95Met Pro Asp Asn Glu Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser
100 105 110Trp Tyr Thr Tyr Ser Pro Thr Gly Ile Arg
115 120<210>277<211>2503<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(2503)<223>RXN01308<400>277tttcctggta cacctactcc ccaacccgca tccgctaccc atacatcggt ggcgtgctag 60ttgatatgtc cgcgaagcca aggaacgcct gggcgatccg gtg ctg gcg tgg cgc 115
Val Leu Ala Trp Arg
1 5gac att gta gaa acc cca gaa aag cgc aaa gca tat gta tcc cag cgg 163Asp Ile Val Glu Thr Pro Glu Lys Arg Lys Ala Tyr Val Ser Gln Arg
10 15 20ggc aaa ggt ggc ctc atc cgc gtt cag tat gag gaa gcc atg gag att 211Gly Lys Gly Gly Leu Ile Arg Val Gln Tyr Glu Glu Ala Met Glu Ile
25 30 35gct gcg gca gcc cat gtg tac acc atc cgc caa tac ggc ccc gac cgc 259Ala Ala Ala Ala His Val Tyr Thr Ile Arg Gln Tyr Gly Pro Asp Arg
40 45 50att cat gga ttc acc gtt att ccc gca atg tcg cag gtg tct tac ggt 307Ile His Gly Phe Thr Val Ile Pro Ala Met Ser Gln Val Ser Tyr Gly
55 60 65gct ggt act cgc ttc ttg cag atg atc ggc gga gtg gcg ctg tcc ttc 355Ala Gly Thr Arg Phe Leu Gln Met Ile Gly Gly Val Ala Leu Ser Phe70 75 80 85tac gat tgg tac gcc gac ctc cca cca gca tca cca caa act ttc ggc 403Tyr Asp Trp Tyr Ala Asp Leu Pro Pro Ala Ser Pro Gln Thr Phe Gly
90 95 100gat caa act gac gtt ccg gaa tct ggc gac tgg tac aac tcc agc tac 451Asp Gln Thr Asp Val Pro Glu Ser Gly Asp Trp Tyr Asn Ser Ser Tyr
105 110 115ctc atg atg tgg ggt tcc aac att ccg gtg acc cgc acg cct gac tcc 499Leu Met Met Trp Gly Ser Asn Ile Pro Val Thr Arg Thr Pro Asp Ser
120 125 130cac ttc atg gtg gaa gcc cgc tac aag ggc acc aag gtt gtt gtg gtt 547His Phe Met Val Glu Ala Arg Tyr Lys Gly Thr Lys Val Val Val Val
135 140 145tcc ccg gat ttc gct gac tcc acc aaa ttt gct gat gaa tgg gca cgc 595Ser Pro Asp Phe Ala Asp Ser Thr Lys Phe Ala Asp Glu Trp Ala Arg150 155 160 165atc cac cct ggt act gac ggc gca ctc gcc ttt gcc atg ggc cat gtg 643Ile His Pro Gly Thr Asp Gly Ala Leu Ala Phe Ala Met Gly His Val
170 175 180atc ttg aag gaa ttc cat gtt gac aag aag acg ccg tac ttc atg gac 691Ile Leu Lys Glu Phe His Val Asp Lys Lys Thr Pro Tyr Phe Met Asp
185 190 195tac atg cgc aaa tac acg gac tct cct ttc ctc gtg gaa tta gat gag 739Tyr Met Arg Lys Tyr Thr Asp Ser Pro Phe Leu Val Glu Leu Asp Glu
200 205 210cac ggc gat ggc acc tac acc cca ggt aaa ttc ctc act gca gac cgc 787His Gly Asp Gly Thr Tyr Thr Pro Gly Lys Phe Leu Thr Ala Asp Arg
215 220 225gca gct gat atc tcc cca gcg ctt gcc gcc act cca aat gcc acc cac 835Ala Ala Asp Ile Ser Pro Ala Leu Ala Ala Thr Pro Asn Ala Thr His230 235 240 245cgt ctc ctt gtg ctg caa aaa gat ggc tca gtt gta gat ccc ggt ggc 883Arg Leu Leu Val Leu Gln Lys Asp Gly Ser Val Val Asp Pro Gly Gly
250 255 260act gtc gcg gac cgt tgg ggt gaa gaa ggc atg ggt aag tgg aat ctg 931Thr Val Ala Asp Arg Trp Gly Glu Glu Gly Met Gly Lys Trp Asn Leu
265 270 275cgc tta gac ggc gta gat cca gtg atg act att gca gat gta cag act 979Arg Leu Asp Gly Val Asp Pro Val Met Thr Ile Ala Asp Val Gln Thr
280 285 290gac acc gaa act gcg gaa gtc ctc ttc ccc cgc ttc gat ctc cca gca 1027Asp Thr Glu Thr Ala Glu Val Leu Phe Pro Arg Phe Asp Leu Pro Ala
295 300 305act gcc acc caa gaa ggc ccc att ggt gct ggc acc atc agc cgg ggc 1075Thr Ala Thr Gln Glu Gly Pro Ile Gly Ala Gly Thr Ile Ser Arg Gly310 315 320 325gtt ccc acc atc acg ttg aat ggc cga aag tac acc act gtc ttt gat 1123Val Pro Thr Ile Thr Leu Asn Gly Arg Lys Tyr Thr Thr Val Phe Asp
330 335 340gtg ttg ctc gca cac tac ggt gtg aac cgc gaa gag ctc aac ctt cct 1171Val Leu Leu Ala His Tyr Gly Val Asn Arg Glu Glu Leu Asn Leu Pro
345 350 355ggt gag tgg cct aag gat ttc cag gat cca gtc atg ggt act cct gcg 1219Gly Glu Trp Pro Lys Asp Phe Gln Asp Pro Val Met Gly Thr Pro Ala
360 365 370tgg cag gaa gag ctc acg ggt gtt cct gct aat cag gcg att cgt ttg 1267Trp Gln Glu Glu Leu Thr Gly Val Pro Ala Asn Gln Ala Ile Arg Leu
375 380 385ggt cgg gaa ttt gct cag aat gct gat gat tcc aag ggc cgt tcc cag 1315Gly Arg Glu Phe Ala Gln Asn Ala Asp Asp Ser Lys Gly Arg Ser Gln390 395 400 405atc atc atg ggt gct ggt gtg aac cac tac ttc cat gcg gat tct att 1363Ile Ile Met Gly Ala Gly Val Asn His Tyr Phe His Ala Asp Ser Ile
410 415 420tat cgc aca ttc ttg gcg ctg acc tct atg tgt ggc acc caa ggt gtt 1411Tyr Arg Thr Phe Leu Ala Leu Thr Ser Met Cys Gly Thr Gln Gly Val
425 430 435aac ggt ggc ggt tgg gct cac tac gtt ggt cag gag aaa ctc cgt cca 1459Asn Gly Gly Gly Trp Ala His Tyr Val Gly Gln Glu Lys Leu Arg Pro
440 445 450atg aat ggt tgg gca cag tat gcc ttt gct aca gac tgg cag cgt cca 1507Met Asn Gly Trp Ala Gln Tyr Ala Phe Ala Thr Asp Trp Gln Arg Pro
455 460 465cca cgt cag atg atc acc act ggt ttc tac tac ctc acc acg gat cag 1555Pro Arg Gln Met Ile Thr Thr Gly Phe Tyr Tyr Leu Thr Thr Asp Gln470 475 480 485tgg agg tat gac aac act cgt gct aat cgt ctg gct tcc cca ctg gct 1603Trp Arg Tyr Asp Asn Thr Arg Ala Asn Arg Leu Ala Ser Pro Leu Ala
490 495 500aat cgt ggc acc gtg ggt gac aaa atg acg gcg gat acc ttg gtg gaa 1651Asn Arg Gly Thr Val Gly Asp Lys Met Thr Ala Asp Thr Leu Val Glu
505 510 515tcc atg aaa cgt gga tgg atg ccg tca ttc ccg caa ttc aac cgc aat 1699Ser Met Lys Arg Gly Trp Met Pro Ser Phe Pro Gln Phe Asn Arg Asn
520 525 530ccc ctc atc ttg agc cag gag gcg gaa gaa aag ggc gtg tct gtt tct 1747Pro Leu Ile Leu Ser Gln Glu Ala Glu Glu Lys Gly Val Ser Val Ser
535 540 545gac cat att gtt cag cag ctc acc gat ggt gac ttg cag ttc gcc tgc 1795Asp His Ile Val Gln Gln Leu Thr Asp Gly Asp Leu Gln Phe Ala Cys550 555 560 565gag gat ccg gat gca ccg gaa aac tgg cca cgc att ctg ctt aac tgg 1843Glu Asp Pro Asp Ala Pro Glu Asn Trp Pro Arg Ile Leu Leu Asn Trp
570 575 580cgc aca aac cta atg ggc tct tca gct aag ggc acg gag ttt ttc ttg 1891Arg Thr Asn Leu Met Gly Ser Ser Ala Lys Gly Thr Glu Phe Phe Leu
585 590 595cgc cat atg ttg ggt gtg gat tct gat gca tct gct gaa gaa aac gcg 1939Arg His Met Leu Gly Val Asp Ser Asp Ala Ser Ala Glu Glu Asn Ala
600 605 610ccg gag gat cgt cca agt tcc att gtg tgg agg gat gag gct ccg gaa 1987Pro Glu Asp Arg Pro Ser Ser Ile Val Trp Arg Asp Glu Ala Pro Glu
615 620 625gga aag ctc gat ttg atg ctg acc acg gat ttc cgc aac act tcc acc 2035Gly Lys Leu Asp Leu Met Leu Thr Thr Asp Phe Arg Asn Thr Ser Thr630 635 640 645acc ttg gtc tcg gat atc gtg ctg ccg gca gcc acc tgg tat gag aag 2083Thr Leu Val Ser Asp Ile Val Leu Pro Ala Ala Thr Trp Tyr Glu Lys
650 655 660cat gat ttg tcc acc acg gat atg cac ccc ttc atc cac tcg ttc aat 2131His Asp Leu Ser Thr Thr Asp Met His Pro Phe Ile His Ser Phe Asn
665 670 675gct gcg atc aac cca ccg tgg gag acg cgt act gac tgg gag gtc ttc 2179Ala Ala Ile Asn Pro Pro Trp Glu Thr Arg Thr Asp Trp Glu Val Phe
680 685 690cac gat ctc acc aaa gaa ttc tcc tca cag gca gca acc tgg ttg ggc 2227His Asp Leu Thr Lys Glu Phe Ser Ser Gln Ala Ala Thr Trp Leu Gly
695 700 705acc caa acc gat gtg atc acc gca ccg att gcc cat gac tcc ccg gat 2275Thr Gln Thr Asp Val Ile Thr Ala Pro Ile Ala His Asp Ser Pro Asp710 715 720 725gag ctc aat atg cct ggc ggt atc gtg cca gat att gat gag gtc ggg 2323Glu Leu Asn Met Pro Gly Gly Iie Val Pro Asp Ile Asp Glu Val Gly
730 735 740ctg atc cct ggc aag acg atg gcc aag atc atc ccg gtg gaa cgt gat 2371Leu Ile Pro Gly Lys Thr Met Ala Lys Ile Ile Pro Val Glu Arg Asp
745 750 755tac tcc aag gtg tat gaa aag tgg aca cac ttg gga cca ctc acc gcc 2419Tyr Ser Lys Val Tyr Glu Lys Trp Thr His Leu Gly Pro Leu Thr Ala
760 765 770aaa gcg ggt acc gga acc cac ggc act gcg ttt aac gtg acc aag caa 2467Lys Ala Gly Thr Gly Thr His Gly Thr Ala Phe Asn Val Thr Lys Gln
775 780 785acc gag gag ctg gcg ctg atc aac ggc acc tcc atc 2503Thr Glu Glu Leu Ala Leu Ile Asn Gly Thr Ser Ile790 795 800<210>278<211>801<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>278Val Leu Ala Trp Arg Asp Ile Val Glu Thr Pro Glu Lys Arg Lys Ala1 5 10 15Tyr Val Ser Gln Arg Gly Lys Gly Gly Leu Ile Arg Val Gln Tyr Glu
20 25 30Glu Ala Met Glu Ile Ala Ala Ala Ala His Val Tyr Thr Ile Arg Gln
35 40 45Tyr Gly Pro Asp Arg Ile His Gly Phe Thr Val Ile Pro Ala Met Ser
50 55 60Gln Val Ser Tyr Gly Ala Gly Thr Arg Phe Leu Gln Met Ile Gly Gly65 70 75 80Val Ala Leu Ser Phe Tyr Asp Trp Tyr Ala Asp Leu Pro Pro Ala Ser
85 90 95Pro Gln Thr Phe Gly Asp Gln Thr Asp Val Pro Glu Ser Gly Asp Trp
100 105 110Tyr Asn Ser Ser Tyr Leu Met Met Trp Gly Ser Asn Ile Pro Val Thr
115 120 125Arg Thr Pro Asp Ser His Phe Met Val Glu Ala Arg Tyr Lys Gly Thr
130 135 140Lys Val Val Val Val Ser Pro Asp Phe Ala Asp Ser Thr Lys Phe Ala145 150 155 160Asp Glu Trp Ala Arg Ile His Pro Gly Thr Asp Gly Ala Leu Ala Phe
165 170 175Ala Met Gly His Val Ile Leu Lys Glu Phe His Val Asp Lys Lys Thr
180 185 190Pro Tyr Phe Met Asp Tyr Met Arg Lys Tyr Thr Asp Ser Pro Phe Leu
195 200 205Val Glu Leu Asp Glu His Gly Asp Gly Thr Tyr Thr Pro Gly Lys Phe
210 215 220Leu Thr Ala Asp Arg Ala Ala Asp Ile Ser Pro Ala Leu Ala Ala Thr225 230 235 240Pro Asn Ala Thr His Arg Leu Leu Val Leu Gln Lys Asp Gly Ser Val
245 250 255Val Asp Pro Gly Gly Thr Val Ala Asp Arg Trp Gly Glu Glu Gly Met
260 265 270Gly Lys Trp Asn Leu Arg Leu Asp Gly Val Asp Pro Val Met Thr Ile
275 280 285Ala Asp Val Gln Thr Asp Thr Glu Thr Ala Glu Val Leu Phe Pro Arg
290 295 300Phe Asp Leu Pro Ala Thr Ala Thr Gln Glu Gly Pro Ile Gly Ala Gly305 310 315 320Thr Ile Ser Arg Gly Val Pro Thr Ile Thr Leu Asn Gly Arg Lys Tyr
325 330 335Thr Thr Val Phe Asp Val Leu Leu Ala His Tyr Gly Val Asn Arg Glu
340 345 350Glu Leu Asn Leu Pro Gly Glu Trp Pro Lys Asp Phe Gln Asp Pro Val
355 360 365Met Gly Thr Pro Ala Trp Gln Glu Glu Leu Thr Gly Val Pro Ala Asn
370 375 380Gln Ala Ile Arg Leu Gly Arg Glu Phe Ala Gln Asn Ala Asp Asp Ser385 390 395 400Lys Gly Arg Ser Gln Ile Ile Met Gly Ala Gly Val Asn His Tyr Phe
405 410 415His Ala Asp Ser Ile Tyr Arg Thr Phe Leu Ala Leu Thr Ser Met Cys
420 425 430Gly Thr Gln Gly Val Asn Gly Gly Gly Trp Ala His Tyr Val Gly Gln
435 440 445Glu Lys Leu Arg Pro Met Asn Gly Trp Ala Gln Tyr Ala Phe Ala Thr
450 455 460Asp Trp Gln Arg Pro Pro Arg Gln Met Ile Thr Thr Gly Phe Tyr Tyr465 470 475 480Leu Thr Thr Asp Gln Trp Arg Tyr Asp Asn Thr Arg Ala Asn Arg Leu
485 490 495Ala Ser Pro Leu Ala Asn Arg Gly Thr Val Gly Asp Lys Met Thr Ala
500 505 510Asp Thr Leu Val Glu Ser Met Lys Arg Gly Trp Met Pro Ser Phe Pro
515 520 525Gln Phe Asn Arg Asn Pro Leu Ile Leu Ser Gln Glu Ala Glu Glu Lys
530 535 540Gly Val Ser Val Ser Asp His Ile Val Gln Gln Leu Thr Asp Gly Asp545 550 555 560Leu Gln Phe Ala Cys Glu Asp Pro Asp Ala Pro Glu Asn Trp Pro Arg
565 570 575Ile Leu Leu Asn Trp Arg Thr Asn Leu Met Gly Ser Ser Ala Lys Gly
580 585 590Thr Glu Phe Phe Leu Arg His Met Leu Gly Val Asp Ser Asp Ala Ser
595 600 605Ala Glu Glu Asn Ala Pro Glu Asp Arg Pro Ser Ser Ile Val Trp Arg
610 615 620Asp Glu Ala Pro Glu Gly Lys Leu Asp Leu Met Leu Thr Thr Asp Phe625 630 635 640Arg Asn Thr Ser Thr Thr Leu Val Ser Asp Ile Val Leu Pro Ala Ala
645 650 655Thr Trp Tyr Glu Lys His Asp Leu Ser Thr Thr Asp Met His Pro Phe
660 665 670Ile His Ser Phe Asn Ala Ala Ile Asn Pro Pro Trp Glu Thr Arg Thr
675 680 685Asp Trp Glu Val Phe His Asp Leu Thr Lys Glu Phe Ser Ser Gln Ala
690 695 700Ala Thr Trp Leu Gly Thr Gln Thr Asp Val Ile Thr Ala Pro Ile Ala705 710 715 720His Asp Ser Pro Asp Glu Leu Asn Met Pro Gly Gly Ile Val Pro Asp
725 730 735Ile Asp Glu Val Gly Leu Ile Pro Gly Lys Thr Met Ala Lys Ile Ile
740 745 750Pro Val Glu Arg Asp Tyr Ser Lys Val Tyr Glu Lys Trp Thr His Leu
755 760 765Gly Pro Leu Thr Ala Lys Ala Gly Thr Gly Thr His Gly Thr Ala Phe
770 775 780Asn Val Thr Lys Gln Thr Glu Glu Leu Ala Leu Ile Asn Gly Thr Ser785 790 795 800Ile<210>279<211>765<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(85)..(765)<223>FRXA01307<400>279ttccccaacc cgcatccgct acccatacat cggtggcgtg ctagttgata tgtccgcgaa 60gccaaggaac gcctgggcga tccg gtg ctg gcg tgg cgc gac att gta gaa acc 114
Val Leu Ala Trp Arg Asp Ile Val Glu Thr
1 5 10cca gaa aag cgc aaa gca tat gta tcc cag cgg ggc aaa ggt ggc ctc 162Pro Glu Lys Arg Lys Ala Tyr Val Ser Gln Arg Gly Lys Gly Gly Leu
15 20 25atc cgc gtt cag tat gag gaa gcc atg gag att gct gcg gca gcc cat 210Ile Arg Val Gln Tyr Glu Glu Ala Met Glu Ile Ala Ala Ala Ala His
30 35 40gtg tac acc atc cgc caa tac ggc ccc gac cgc att cat gga ttc acc 258Val Tyr Thr Ile Arg Gln Tyr Gly Pro Asp Arg Ile His Gly Phe Thr
45 50 55gtt att ccc gca atg tcg cag gtg tct tac ggt gct ggt act cgc ttc 306Val Ile Pro Ala Met Ser Gln Val Ser Tyr Gly Ala Gly Thr Arg Phe
60 65 70ttg cag atg atc ggc gga gtg gcg ctg tcc ttc tac gat tgg tac gcc 354Leu Gln Met Ile Gly Gly Val Ala Leu Ser Phe Tyr Asp Trp Tyr Ala75 80 85 90gac ctc cca cca gca tca cca caa act ttc ggc gat caa act gac gtt 402Asp Leu Pro Pro Ala Ser Pro Gln Thr Phe Gly Asp Gln Thr Asp Val
95 100 105ccg gaa tct ggc gac tgg tac aac tcc agc tac ctc atg atg tgg ggt 450Pro Glu Ser Gly Asp Trp Tyr Asn Ser Ser Tyr Leu Met Met Trp Gly
110 115 120tcc aac att ccg gtg acc cgc acg cct gac tcc cac ttc atg gtg gaa 498Ser Asn Ile Pro Val Thr Arg Thr Pro Asp Ser His Phe Met Val Glu
125 130 135gcc cgc tac aag ggc acc aag gtt gtt gtg gtt tcc ccg gat ttc gct 546Ala Arg Tyr Lys Gly Thr Lys Val Val Val Val Ser Pro Asp Phe Ala
140 145 150gac tcc acc aaa ttt gct gat gaa tgg gca cgc atc cac cct ggt act 594Asp Ser Thr Lys Phe Ala Asp Glu Trp Ala Arg Ile His Pro Gly Thr155 160 165 170gac ggc gca ctc gcc ttt gcc atg ggc cat gtg atc ttg aag gaa ttc 642Asp Gly Ala Leu Ala Phe Ala Met Gly His Val Ile Leu Lys Glu Phe
175 180 185cat gtt gac aag aag acg ccg tac ttc atg gac tac atg cgc aaa tac 690His Val Asp Lys Lys Thr Pro Tyr Phe Met Asp Tyr Met Arg Lys Tyr
190 195 200acg gac tct cct ttc ctc gtg gaa tta gat gag cac ggc gat ggc acc 738Thr Asp Ser Pro Phe Leu Val Glu Leu Asp Glu His Gly Asp Gly Thr
205 210 215tac acc cca ggt aaa ttc ctc act gca 765Tyr Thr Pro Gly Lys Phe Leu Thr Ala
220 225<210>280<211>227<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>280Val Leu Ala Trp Arg Asp Ile Val Glu Thr Pro Glu Lys Arg Lys Ala1 5 10 15Tyr Val Ser Gln Arg Gly Lys Gly Gly Leu Ile Arg Val Gln Tyr Glu
20 25 30Glu Ala Met Glu Ile Ala Ala Ala Ala His Val Tyr Thr Ile Arg Gln
35 40 45Tyr Gly Pro Asp Arg Ile His Gly Phe Thr Val Ile Pro Ala Met Ser
50 55 60Gln Val Ser Tyr Gly Ala Gly Thr Arg Phe Leu Gln Met Ile Gly Gly65 70 75 80Val Ala Leu Ser Phe Tyr Asp Trp Tyr Ala Asp Leu Pro Pro Ala Ser
85 90 95Pro Gln Thr Phe Gly Asp Gln Thr Asp Val Pro Glu Ser Gly Asp Trp
100 105 110Tyr Asn Ser Ser Tyr Leu Met Met Trp Gly Ser Asn Ile Pro Val Thr
115 120 125Arg Thr Pro Asp Ser His Phe Met Val Glu Ala Arg Tyr Lys Gly Thr
130 135 140Lys Val Val Val Val Ser Pro Asp Phe Ala Asp Ser Thr Lys Phe Ala145 150 155 160Asp Glu Trp Ala Arg Ile His Pro Gly Thr Asp Gly Ala Leu Ala Phe
165 170 175Ala Met Gly His Val Ile Leu Lys Glu Phe His Val Asp Lys Lys Thr
180 185 190Pro Tyr Phe Met Asp Tyr Met Arg Lys Tyr Thr Asp Ser Pro Phe Leu
195 200 205Val Glu Leu Asp Glu His Gly Asp Gly Thr Tyr Thr Pro Gly Lys Phe
210 215 220Leu Thr Ala225<210>281<211>1206<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(1206)<223>FRXA01308<400>281gcc gcc act cca aat gcc acc cac cgt ctc ctt gtg ctg caa aaa gat 48Ala Ala Thr Pro Asn Ala Thr His Arg Leu Leu Val Leu Gln Lys Asp1 5 10 15ggc tca gtt gta gat ccc ggt ggc act gtc gcg gac cgt tgg ggt gaa 96Gly Ser Val Val Asp Pro Gly Gly Thr Val Ala Asp Arg Trp Gly Glu
20 25 30gaa ggc atg ggt aag tgg aat ctg cgc tta gac ggc gta gat cca gtg 144Glu Gly Met Gly Lys Trp Asn Leu Arg Leu Asp Gly Val Asp Pro Val
35 40 45atg act att gca gat gta cag act gac acc gaa act gcg gaa gtc ctc 192Met Thr Ile Ala Asp Val Gln Thr Asp Thr Glu Thr Ala Glu Val Leu
50 55 60ttc ccc cgc ttc gat ctc cca gca act gcc acc caa gaa ggc ccc att 240Phe Pro Arg Phe Asp Leu Pro Ala Thr Ala Thr Gln Glu Gly Pro Ile65 70 75 80ggt gct ggc acc atc agc cgg ggc gtt ccc acc atc acg ttg aat ggc 288Gly Ala Gly Thr Ile Ser Arg Gly Val Pro Thr Ile Thr Leu Asn Gly
85 90 95cga aag tac acc act gtc ttt gat gtg ttg ctc gca cac tac ggt gtg 336Arg Lys Tyr Thr Thr Val Phe Asp Val Leu Leu Ala His Tyr Gly Val
100 105 110aac cgc gaa gag ctc aac ctt cct ggt gag tgg cct aag gat ttc cag 384Asn Arg Glu Glu Leu Asn Leu Pro Gly Glu Trp Pro Lys Asp Phe Gln
115 120 125gat cca gtc atg ggt act cct gcg tgg cag gaa gag ctc acg ggt gtt 432Asp Pro Val Met Gly Thr Pro Ala Trp Gln Glu Glu Leu Thr Gly Val
130 135 140cct gct aat cag gcg att cgt ttg ggt cgg gaa ttt gct cag aat gct 480Pro Ala Asn Gln Ala Ile Arg Leu Gly Arg Glu Phe Ala Gln Asn Ala145 150 155 160gat gat tcc aag ggc cgt tcc cag atc atc atg ggt gct ggt gtg aac 528Asp Asp Ser Lys Gly Arg Ser Gln Ile Ile Met Gly Ala Gly Val Asn
165 170 175cac tac ttc cat gcg gat tct att tat cgc aca ttc ttg gcg ctg acc 576His Tyr Phe His Ala Asp Ser Ile Tyr Arg Thr Phe Leu Ala Leu Thr
180 185 190tct atg tgt ggc acc caa ggt gtt aac ggt ggc ggt tgg gct cac tac 624Ser Met Cys Gly Thr Gln Gly Val Asn Gly Gly Gly Trp Ala His Tyr
195 200 205gtt ggt cag gag aaa ctc cgt cca atg aat ggt tgg gca cag tat gcc 672Val Gly Gln Glu Lys Leu Arg Pro Met Asn Gly Trp Ala Gln Tyr Ala
210 215 220ttt gct aca gac tgg cag cgt cca cca cgt cag atg atc acc act ggt 720Phe Ala Thr Asp Trp Gln Arg Pro Pro Arg Gln Met Ile Thr Thr Gly225 230 235 240ttc tac tac ctc acc acg gat cag tgg agg tat gac aac act cgt gct 768Phe Tyr Tyr Leu Thr Thr Asp Gln Trp Arg Tyr Asp Asn Thr Arg Ala
245 250 255aat cgt ctg gct tcc cca ctg gct aat cgt ggc acc gtg ggt gac aaa 816Asn Arg Leu Ala Ser Pro Leu Ala Asn Arg Gly Thr Val Gly Asp Lys
260 265 270atg acg gcg gat acc ttg gtg gaa tcc atg aaa cgt gga tgg atg ccg 864Met Thr Ala Asp Thr Leu Val Glu Ser Met Lys Arg Gly Trp Met Pro
275 280 285tca ttc ccg caa ttc aac cgc aat ccc ctc atc ttg agc cag gag gcg 912Ser Phe Pro Gln Phe Asn Arg Asn Pro Leu Ile Leu Ser Gln Glu Ala
290 295 300gaa gaa aag ggc gtg tct gtt tct gac cat att gtt cag cag ctc acc 960Glu Glu Lys Gly Val Ser Val Ser Asp His Ile Val Gln Gln Leu Thr305 310 315 320gat ggt gac ttg cag ttc gcc tgc gag gat ccg gat gca ccg gaa aac 1008Asp Gly Asp Leu Gln Phe Ala Cys Glu Asp Pro Asp Ala Pro Glu Asn
325 330 335tgg cca cgc att ctg ctt aac tgg cgc aca aac cta atg ggc tct tca 1056Trp Pro Arg Ile Leu Leu Asn Trp Arg Thr Asn Leu Met Gly Ser Ser
340 345 350gct aag ggc acg gag ttt ttc ttg cgc cat atg ttg ggt gtg gat tct 1104Ala Lys Gly Thr Glu Phe Phe Leu Arg His Met Leu Gly Val Asp Ser
355 360 365gat gca tct gct gaa aaa aac gcg ccg gag gat cgt cca agt tcc att 1152Asp Ala Ser Ala Glu Lys Asn Ala Pro Glu Asp Arg Pro Ser Ser Ile
370 375 380gtg tgg agg gat gaa gct tcc gaa ggg aag ctc gat ttg atg ctg acc 1200Val Trp Arg Asp Glu Ala Ser Glu Gly Lys Leu Asp Leu Met Leu Thr385 390 395 400acg gat 1206Thr Asp<210>282<211>402<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>282Ala Ala Thr Pro Asn Ala Thr His Arg Leu Leu Val Leu Gln Lys Asp1 5 10 15Gly Ser Val Val Asp Pro Gly Gly Thr Val Ala Asp Arg Trp Gly Glu
20 25 30Glu Gly Met Gly Lys Trp Asn Leu Arg Leu Asp Gly Val Asp Pro Val
35 40 45Met Thr Ile Ala Asp Val Gln Thr Asp Thr Glu Thr Ala Glu Val Leu
50 55 60Phe Pro Arg Phe Asp Leu Pro Ala Thr Ala Thr Gln Glu Gly Pro Ile65 70 75 80Gly Ala Gly Thr Ile Ser Arg Gly Val Pro Thr Ile Thr Leu Asn Gly
85 90 95Arg Lys Tyr Thr Thr Val Phe Asp Val Leu Leu Ala His Tyr Gly Val
100 105 110Asn Arg Glu Glu Leu Asn Leu Pro Gly Glu Trp Pro Lys Asp Phe Gln
115 120 125Asp Pro Val Met Gly Thr Pro Ala Trp Gln Glu Glu Leu Thr Gly Val
130 135 140Pro Ala Asn Gln Ala Ile Arg Leu Gly Arg Glu Phe Ala Gln Asn Ala145 150 155 160Asp Asp Ser Lys Gly Arg Ser Gln Ile Ile Met Gly Ala Gly Val Asn
165 170 175His Tyr Phe His Ala Asp Ser Ile Tyr Arg Thr Phe Leu Ala Leu Thr
180 185 190Ser Met Cys Gly Thr Gln Gly Val Asn Gly Gly Gly Trp Ala His Tyr
195 200 205Val Gly Gln Glu Lys Leu Arg Pro Met Asn Gly Trp Ala Gln Tyr Ala
210 215 220Phe Ala Thr Asp Trp Gln Arg Pro Pro Arg Gln Met Ile Thr Thr Gly225 230 235 240Phe Tyr Tyr Leu Thr Thr Asp Gln Trp Arg Tyr Asp Asn Thr Arg Ala
245 250 255Asn Arg Leu Ala Ser Pro Leu Ala Asn Arg Gly Thr Val Gly Asp Lys
260 265 270Met Thr Ala Asp Thr Leu Val Glu Ser Met Lys Arg Gly Trp Met Pro
275 280 285Ser Phe Pro Gln Phe Asn Arg Asn Pro Leu Ile Leu Ser Gln Glu Ala
290 295 300Glu Glu Lys Gly Val Ser Val Ser Asp His Ile Val Gln Gln Leu Thr305 310 315 320Asp Gly Asp Leu Gln Phe Ala Cys Glu Asp Pro Asp Ala Pro Glu Asn
325 330 335Trp Pro Arg Ile Leu Leu Asn Trp Arg Thr Asn Leu Met Gly Ser Ser
340 345 350Ala Lys Gly Thr Glu Phe Phe Leu Arg His Met Leu Gly Val Asp Ser
355 360 365Asp Ala Ser Ala Glu Lys Asn Ala Pro Glu Asp Arg Pro Ser Ser Ile
370 375 380Val Trp Arg Asp Glu Ala Ser Glu Gly Lys Leu Asp Leu Met Leu Thr385 390 395 400Thr Asp<210>283<211>824<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(801)<223>RXN01309<400>283att gca gac cac gaa ggt acc cac atc aat tgg gac atg gtc aaa gaa 48Ile Ala Asp His Glu Gly Thr His Ile Asn Trp Asp Met Val Lys Glu1 5 10 15cgt tcc gcc gag gtg atc acc tca ccg gag tgg act ggt tcc aag aag 96Arg Ser Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly Ser Lys Lys
20 25 30gac gga cgt cgc tac acc gcg ttt tcc atc aac att gaa tac gac aag 144Asp Gly Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu Tyr Asp Lys
35 40 45ccg tgg cac acc ctg tct ggt cgc atg cac tac tac ctc gac cac gat 192Pro Trp His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu Asp His Asp
50 55 60tgg ttt att gat tac ggc gag cag ttg cca atc ttt agg cca ccg ttg 240Trp Phe Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg Pro Pro Leu65 70 75 80gac aag atc cac atc aat ggt gag gtc ggc cct ggc cag tcg gtc aca 288Asp Lys Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln Ser Val Thr
85 90 95ggc acc gac ggc gaa cca gaa gta acc gtg cgt tat ctg acc acc cac 336Gly Thr Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu Thr Thr His
100 105 110aac aag tgg tcg att cac tcg cag tac tac gac aat ctg cat gtg ctt 384Asn Lys Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu His Val Leu
115 120 125tct att tct cgt ggc ggc cag gtg atc tgg atg tcc aac aag gat gca 432Ser Ile Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn Lys Asp Ala
130 135 140gag aaa ctc ggt atc gct gac aac gat tgg atc gag gct tat aac cgc 480Glu Lys Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala Tyr Asn Arg145 150 155 160aac ggc gtt gtt tct gct cgt gcg att gtc tcc cac cgc att cct gaa 528Asn Gly Val Val Ser Ala Arg Ala Ile Val Ser His Arg Ile Pro Glu
165 170 175ggc acc gtg ttt atg aac cac gcg cag gaa cgc acc gct ggc acc ccg 576Gly Thr Val Phe Met Asn His Ala Gln Glu Arg Thr Ala Gly Thr Pro
180 185 190ctg aac gag aag tct ggc agg cgc ggc gga act cac aac tct ctt act 624Leu Asn Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn Ser Leu Thr
195 200 205cga atc atg att aag ccg gtc cat gtt gcc ggt ggc tac ggc cac tta 672Arg Ile Met Ile Lys Pro Val His Val Ala Gly Gly Tyr Gly His Leu
210 215 220acc tat ggc ttc aac tac atc ggc caa ccg gaa ata acc gcg atg agg 720Thr Tyr Gly Phe Asn Tyr Ile Gly Gln Pro Glu Ile Thr Ala Met Arg225 230 235 240tca cca gaa ttc gtc gcc gct ccc agg agg tgc agt act aat gaa ggt 768Ser Pro Glu Phe Val Ala Ala Pro Arg Arg Cys Ser Thr Asn Glu Gly
245 250 255cat ggc tca gat cgc aat gat cat gaa ctt gga taagtgcatt ggctgccaca 821His Gly Ser Asp Arg Asn Asp His Glu Leu Gly
260 265cgt 824<210>284<211>267<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>284Ile Ala Asp His Glu Gly Thr His Ile Asn Trp Asp Met Val Lys Glu1 5 10 15Arg Ser Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly Ser Lys Lys
20 25 30Asp Gly Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu Tyr Asp Lys
35 40 45Pro Trp His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu Asp His Asp
50 55 60Trp Phe Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg Pro Pro Leu65 70 75 80Asp Lys Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln Ser Val Thr
85 90 95Gly Thr Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu Thr Thr His
100 105 110Asn Lys Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu His Val Leu
115 120 125Ser Ile Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn Lys Asp Ala
130 135 140Glu Lys Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala Tyr Asn Arg145 150 155 160Asn Gly Val Val Ser Ala Arg Ala Ile Val Ser His Arg Ile Pro Glu
165 170 175Gly Thr Val Phe Met Asn His Ala Gln Glu Arg Thr Ala Gly Thr Pro
180 185 190Leu Asn Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn Ser Leu Thr
195 200 205Arg Ile Met Ile Lys Pro Val His Val Ala Gly Gly Tyr Gly His Leu
210 215 220Thr Tyr Gly Phe Asn Tyr Ile Gly Gln Pro Glu Ile Thr Ala Met Arg225 230 235 240Ser Pro Glu Phe Val Ala Ala Pro Arg Arg Cys Ser Thr Asn Glu Gly
245 250 255His Gly Ser Asp Arg Asn Asp His Glu Leu Gly
260 265<210>285<211>692<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(669)<223>FRXA01309<400>285att gca gac cac gaa ggt acc cac atc aat tgg gac atg gtc aaa gaa 48Ile Ala Asp His Glu Gly Thr His Ile Asn Trp Asp Met Val Lys Glu1 5 10 15cgt tcc gcc gag gtg atc acc tca ccg gag tgg act ggt tcc aag aag 96Arg Ser Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly Ser Lys Lys
20 25 30gac gga cgt cgc tac acc gcg ttt tcc atc aac att gaa tac gac aag 144Asp Gly Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu Tyr Asp Lys
35 40 45ccg tgg cac acc ctg tct ggt cgc atg cac tac tac ctc gac cac gat 192Pro Trp His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu Asp His Asp
50 55 60tgg ttt att gat tac ggc gag cag ttg cca atc ttt agg cca ccg ttg 240Trp Phe Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg Pro Pro Leu65 70 75 80gac aag atc cac atc aat ggt gag gtc ggc cct ggc cag tcg gtc aca 288Asp Lys Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln Ser Val Thr
85 90 95ggc acc gac ggc gaa cca gaa gta acc gtg cgt tat ctg acc acc cac 336Gly Thr Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu Thr Thr His
100 105 110aac aag tgg tcg att cac tcg cag tac tac gac aat ctg cat gtg ctt 384Asn Lys Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu His Val Leu
115 120 125tct att tct cgt ggc ggc cag gtg atc tgg atg tcc aac aag gat gca 432Ser Ile Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn Lys Asp Ala
130 135 140gag aaa ctc ggt atc gct gac aac gat tgg atc gag gct tat aac cgc 480Glu Lys Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala Tyr Asn Arg145 150 155 160aac ggc gtt gtt tct gct cgt gcg att gtc tcc cac cgc att cct gaa 528Asn Gly Val Val Ser Ala Arg Ala Ile Val Ser His Arg Ile Pro Glu
165 170 175ggc acc gtg ttt atg aac cac gcg cag gaa ccc acc gct ggc acc ccg 576Gly Thr Val Phe Met Asn His Ala Gln Glu Pro Thr Ala Gly Thr Pro
180 185 190ctg aac gag aag tct ggc agg cgc ggc gga act cac aac tct ctt act 624Leu Asn Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn Ser Leu Thr
195 200 205cga atc atg att aaa acg gtc cat gtt gcc ggt ggc tac ggc act 669Arg Ile Met Ile Lys Thr Val His Val Ala Gly Gly Tyr Gly Thr
210 215 220taacctatgg cttaactaca tcg 692<210>286<211>223<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>286Ile Ala Asp His Glu Gly Thr His Ile Asn Trp Asp Met Val Lys Glu1 5 10 15Arg Ser Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly Ser Lys Lys
20 25 30Asp Gly Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu Tyr Asp Lys
35 40 45Pro Trp His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu Asp His Asp
50 55 60Trp Phe Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg Pro Pro Leu65 70 75 80Asp Lys Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln Ser Val Thr
85 90 95Gly Thr Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu Thr Thr His
100 105 110Asn Lys Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu His Val Leu
115 120 125Ser Ile Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn Lys Asp Ala
130 135 140Glu Lys Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala Tyr Asn Arg145 150 155 160Asn Gly Val Val Ser Ala Arg Ala Ile Val Ser His Arg Ile Pro Glu
165 170 175Gly Thr Val Phe Met Asn His Ala Gln Glu Pro Thr Ala Gly Thr Pro
180 185 190Leu Asn Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn Ser Leu Thr
195 200 205Arg Ile Met Ile Lys Thr Val His Val Ala Gly Gly Tyr Gly Thr
210 215 220<210>287<211>807<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(784)<223>RXA02017<400>287gcctgtggca cgggagctcc cagcggcaag gtgagtctga cctcgtggaa cgttggcgaa 60cgccccgctg cgatgttccc accaaggaag gactaggcgg atg cgc acc cat act 115
Met Arg Thr His Thr
1 5ggc aaa att ccg gat cac ttt gtg cct cgc atc tcc atg acg gag gag 163Gly Lys Ile Pro Asp His Phe Val Pro Arg Ile Ser Met Thr Glu Glu
10 15 20cag cgg cgc gtg gtg ttc atg ctg aat agt ttg ctg ttg gat tat cca 211Gln Arg Arg Val Val Phe Met Leu Asn Ser Leu Leu Leu Asp Tyr Pro
25 30 35gag gag gga ttc gtc gac aag cta aat gcc gtc gag gcg cag ctt gat 259Glu Glu Gly Phe Val Asp Lys Leu Asn Ala Val Glu Ala Gln Leu Asp
40 45 50gtc ctt ccg ctc ccc gtc gcg gcg cac gtg gtc gag ttc ctt gac gcg 307Val Leu Pro Leu Pro Val Ala Ala His Val Val Glu Phe Leu Asp Ala
55 60 65gca cgc gtc gct ggg cta cgc gcc atg cag gaa gcc tac gtt gag acc 355Ala Arg Val Ala Gly Leu Arg Ala Met Gln Glu Ala Tyr Val Glu Thr70 75 80 85ttt gac cag cgc cga cgc tgc tca ctg ttt ctc acc tac tac gct gtg 403Phe Asp Gln Arg Arg Arg Cys Ser Leu Phe Leu Thr Tyr Tyr Ala Val
90 95 100ggc gac acc cgg cag cgc ggc acg gcg atc ctc acc ttc cgt caa acg 451Gly Asp Thr Arg Gln Arg Gly Thr Ala Ile Leu Thr Phe Arg Gln Thr
105 110 115ctg caa cag ctc gga ttt gaa tcc gag cgc gac gaa ttg ccc gac cac 499Leu Gln Gln Leu Gly Phe Glu Ser Glu Arg Asp Glu Leu Pro Asp His
120 125 130ctc tgc gtc gtg ctt gag gcc gca gcg ctt gct gat tct tcg ctt ttc 547Leu Cys Val Val Leu Glu Ala Ala Ala Leu Ala Asp Ser Ser Leu Phe
135 140 145gac gcc gcc acc cag gtg tta tca gct cac cgc gac ggc atc gaa gtg 595Asp Ala Ala Thr Gln Val Leu Ser Ala His Arg Asp Gly Ile Glu Val150 155 160 165ttg cgc gca gcc ctc gac aac ctc gac tcg ccc tac aga tac ctg atc 643Leu Arg Ala Ala Leu Asp Asn Leu Asp Ser Pro Tyr Arg Tyr Leu Ile
170 175 180atg tct ttg tgc cag gca ttg cca gaa atc gat gaa gaa acc gcc aac 691Met Ser Leu Cys Gln Ala Leu Pro Glu Ile Asp Glu Glu Thr Ala Asn
185 190 195agc tac atg gag ctc atc cgc agc ggt cca cca gca gaa atg gtg ggc 739Ser Tyr Met Glu Leu Ile Arg Ser Gly Pro Pro Ala Glu Met Val Gly
200 205 210atc ggc acg cct cta ccg ttc ccc acc tca caa ccg gac att cac 784Ile Gly Thr Pro Leu Pro Phe Pro Thr Ser Gln Pro Asp Ile His
215 220 225taggacacac tatgtcaaac ttt 807<210>288<211>228<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>288Met Arg Thr His Thr Gly Lys Ile Pro Asp His Phe Val Pro Arg Ile1 5 10 15Ser Met Thr Glu Glu Gln Arg Arg Val Val Phe Met Leu Asn Ser Leu
20 25 30Leu Leu Asp Tyr Pro Glu Glu Gly Phe Val Asp Lys Leu Asn Ala Val
35 40 45Glu Ala Gln Leu Asp Val Leu Pro Leu Pro Val Ala Ala His Val Val
50 55 60Glu Phe Leu Asp Ala Ala Arg Val Ala Gly Leu Arg Ala Met Gln Glu65 70 75 80Ala Tyr Val Glu Thr Phe Asp Gln Arg Arg Arg Cys Ser Leu Phe Leu
85 90 95Thr Tyr Tyr Ala Val Gly Asp Thr Arg Gln Arg Gly Thr Ala Ile Leu
100 105 110Thr Phe Arg Gln Thr Leu Gln Gln Leu Gly Phe Glu Ser Glu Arg Asp
115 120 125Glu Leu Pro Asp His Leu Cys Val Val Leu Glu Ala Ala Ala Leu Ala
130 135 140Asp Ser Ser Leu Phe Asp Ala Ala Thr Gln Val Leu Ser Ala His Arg145 150 155 160Asp Gly Ile Glu Val Leu Arg Ala Ala Leu Asp Asn Leu Asp Ser Pro
165 170 175Tyr Arg Tyr Leu Ile Met Ser Leu Cys Gln Ala Leu Pro Glu Ile Asp
180 185 190Glu Glu Thr Ala Asn Ser Tyr Met Glu Leu Ile Arg Ser Gly Pro Pro
195 200 205Ala Glu Met Val Gly Ile Gly Thr Pro Leu Pro Phe Pro Thr Ser Gln
210 215 220Pro Asp Ile His225<210>289<211>1073<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(1050)<223>RXA02018<400>289cgc atc tgc gag cac tgc ctc aac ccc acc tgt gtg tcc tcc tgc cca 48Arg Ile Cys Glu His Cys Leu Asn Pro Thr Cys Val Ser Ser Cys Pro1 5 10 15tcc ggt gct atg tac aaa cgc gcc gaa gac ggc atc gtg ctg gtt gac 96Ser Gly Ala Met Tyr Lys Arg Ala Glu Asp Gly Ile Val Leu Val Asp
20 25 30cag gat caa tgc cgt ggc tgg cgc atg tgt gtt tcc ggc tgc ccc tac 144Gln Asp Gln Cys Arg Gly Trp Arg Met Cys Val Ser Gly Cys Pro Tyr
35 40 45aaa aag gtc tac ttc aat cac aaa tcg ggc aag gcc gaa aag tgt acg 192Lys Lys Val Tyr Phe Asn His Lys Ser Gly Lys Ala Glu Lys Cys Thr
50 55 60ctg tgc tat ccg cgc ctc gag gtc ggc cag ccg acc gtg tgc tcc gag 240Leu Cys Tyr Pro Arg Leu Glu Val Gly Gln Pro Thr Val Cys Ser Glu65 70 75 80acg tgc gtg ggt cgc ttg cgc tac ttg ggc gtt ttg ctt tac gac gcc 288Thr Cys Val Gly Arg Leu Arg Tyr Leu Gly Val Leu Leu Tyr Asp Ala
85 90 95gac cgt gtc gct gaa gtc gcc gcc acg cca gac gaa aag gat ctt ttc 336Asp Arg Val Ala Glu Val Ala Ala Thr Pro Asp Glu Lys Asp Leu Phe
100 105 110gaa gcc caa aag acc ctc ttc cta gat ccc cac gac cca cag gtg atc 384Glu Ala Gln Lys Thr Leu Phe Leu Asp Pro His Asp Pro Gln Val Ile
115 120 125gcc gac gcc caa cgc aac ggc atc ccg cac tcc tgg ctc gaa gct gcg 432Ala Asp Ala Gln Arg Asn Gly Ile Pro His Ser Trp Leu Glu Ala Ala
130 135 140cag aac tct cca att tac gat ctc atc ttc aaa tac gag gtt gcc ctc 480Gln Asn Ser Pro Ile Tyr Asp Leu Ile Phe Lys Tyr Glu Val Ala Leu145 150 155 160ccg ctt cac cct gaa tac cgc acc ttg ccg atg gtt tgg tac att ccg 528Pro Leu His Pro Glu Tyr Arg Thr Leu Pro Met Val Trp Tyr Ile Pro
165 170 175cca cta agc ccc atc gtt gat gag gtg acc gcg tcc ggc aac gac ggc 576Pro Leu Ser Pro Ile Val Asp Glu Val Thr Ala Ser Gly Asn Asp Gly
180 185 190gaa gac cac aag atc ctg ctc acc gcg ctg tcc acc atg cgc atc ccg 624Glu Asp His Lys Ile Leu Leu Thr Ala Leu Ser Thr Met Arg Ile Pro
195 200 205ctg gaa tac ctg gct gga ttg ttc act gcc ggt gat acc agg ccg gtg 672Leu Glu Tyr Leu Ala Gly Leu Phe Thr Ala Gly Asp Thr Arg Pro Val
210 215 220gaa aaa tcc ctc cga cgc cta gcc gcc atg cga tca tat atg cgc gat 720Glu Lys Ser Leu Arg Arg Leu Ala Ala Met Arg Ser Tyr Met Arg Asp225 230 235 240atc agt ttg ggc cgc gaa cct cag gaa gaa atc gca gag gct gtc gga 768Ile Ser Leu Gly Arg Glu Pro Gln Glu Glu Ile Ala Glu Ala Val Gly
245 250 255atg acc ggc aag gtg gtg cag gaa atg tat cgc atc ctg gcc att gcc 816Met Thr Gly Lys Val Val Gln Glu Met Tyr Arg Ile Leu Ala Ile Ala
260 265 270aag tat gac gat cgc tat gtc atc ccc acc gcc tcc cct gag acc ccg 864Lys Tyr Asp Asp Arg Tyr Val Ile Pro Thr Ala Ser Pro Glu Thr Pro
275 280 285cgc gga att tct tcc ctg gat cct ttc ggc gat gtc gat cca gcc cga 912Arg Gly Ile Ser Ser Leu Asp Pro Phe Gly Asp Val Asp Pro Ala Arg
290 295 300gcc acc gag cag ctc aac atc ggt ttg ggc gaa ggc gct cca gag gcc 960Ala Thr Glu Gln Leu Asn Ile Gly Leu Gly Glu Gly Ala Pro Glu Ala305 310 315 320tgt ggc acg gga gct ccc agc ggc aag gtg agt ctg acc tcg tgg aac 1008Cys Gly Thr Gly Ala Pro Ser Gly Lys Val Ser Leu Thr Ser Trp Asn
325 330 335gtt ggc gaa cgc ccc gct gcg atg ttc cca cca agg aag gac 1050Val Gly Glu Arg Pro Ala Ala Met Phe Pro Pro Arg Lys Asp
340 345 350taggcggatg cgcacccata ctg 1073<210>290<211>350<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>290Arg Ile Cys Glu His Cys Leu Asn Pro Thr Cys Val Ser Ser Cys Pro1 5 10 15Ser Gly Ala Met Tyr Lys Arg Ala Glu Asp Gly Ile Val Leu Val Asp
20 25 30Gln Asp Gln Cys Arg Gly Trp Arg Met Cys Val Ser Gly Cys Pro Tyr
35 40 45Lys Lys Val Tyr Phe Asn His Lys Ser Gly Lys Ala Glu Lys Cys Thr
50 55 60Leu Cys Tyr Pro Arg Leu Glu Val Gly Gln Pro Thr Val Cys Ser Glu65 70 75 80Thr Cys Val Gly Arg Leu Arg Tyr Leu Gly Val Leu Leu Tyr Asp Ala
85 90 95Asp Arg Val Ala Glu Val Ala Ala Thr Pro Asp Glu Lys Asp Leu Phe
100 105 110Glu Ala Gln Lys Thr Leu Phe Leu Asp Pro His Asp Pro Gln Val Ile
115 120 125Ala Asp Ala Gln Arg Asn Gly Ile Pro His Ser Trp Leu Glu Ala Ala
130 135 140Gln Asn Ser Pro Ile Tyr Asp Leu Ile Phe Lys Tyr Glu Val Ala Leu145 150 155 160Pro Leu His Pro Glu Tyr Arg Thr Leu Pro Met Val Trp Tyr Ile Pro
165 170 175Pro Leu Ser Pro Ile Val Asp Glu Val Thr Ala Ser Gly Asn Asp Gly
180 185 190Glu Asp His Lys Ile Leu Leu Thr Ala Leu Ser Thr Met Arg Ile Pro
195 200 205Leu Glu Tyr Leu Ala Gly Leu Phe Thr Ala Gly Asp Thr Arg Pro Val
210 215 220Glu Lys Ser Leu Arg Arg Leu Ala Ala Met Arg Ser Tyr Met Arg Asp225 230 235 240Ile Ser Leu Gly Arg Glu Pro Gln Glu Glu Ile Ala Glu Ala Val Gly
245 250 255Met Thr Gly Lys Val Val Gln Glu Met Tyr Arg Ile Leu Ala Ile Ala
260 265 270Lys Tyr Asp Asp Arg Tyr Val Ile Pro Thr Ala Ser Pro Glu Thr Pro
275 280 285Arg Gly Ile Ser Ser Leu Asp Pro Phe Gly Asp Val Asp Pro Ala Arg
290 295 300Ala Thr Glu Gln Leu Asn Ile Gly Leu Gly Glu Gly Ala Pro Glu Ala305 310 315 320Cys Gly Thr Gly Ala Pro Ser Gly Lys Val Ser Leu Thr Ser Trp Asn
325 330 335Val Gly Glu Arg Pro Ala Ala Met Phe Pro Pro Arg Lys Asp
340 345 350<210>291<211>900<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(877)<223>RXA02016<400>291acatggagct catccgcagc ggtccaccag cagaaatggt gggcatcggc acgcctctac 60cgttccccac ctcacaaccg gacattcact aggacacact atg tca aac ttt gaa 115
Met Ser Asn Phe Glu
1 5acg ttc ctc tgg gtt gcc tac ccc tgg ctg tgt atc gcc gcc tac atc 163Thr Phe Leu Trp Val Ala Tyr Pro Trp Leu Cys Ile Ala Ala Tyr Ile
10 15 20atc ggc att tct tgg cgc tgg cgc gcc gac caa ttc ggt tgg acc acc 211Ile Gly Ile Ser Trp Arg Trp Arg Ala Asp Gln Phe Gly Trp Thr Thr
25 30 35cac tcc tcc caa atc tac gaa tcc aaa ctc ctc cgc atc gcc tcc cca 259His Ser Ser Gln Ile Tyr Glu Ser Lys Leu Leu Arg Ile Ala Ser Pro
40 45 50ctc ttc cac tgg ggc atg gtg ttc gtg gtg atc ggc cac ctc atg gga 307Leu Phe His Trp Gly Met Val Phe Val Val Ile Gly His Leu Met Gly
55 60 65ctt gcc atc ccc aag agc tgg acc caa gct gta gga att tct gac gcc 355Leu Ala Ile Pro Lys Ser Trp Thr Gln Ala Val Gly Ile Ser Asp Ala70 75 80 85gct tac cac ctc atc gcc acc atc cca ggc acc att gcc ggc atc gct 403Ala Tyr His Leu Ile Ala Thr Ile Pro Gly Thr Ile Ala Gly Ile Ala
90 95 100gca gtc ctt gga ctc atc ggc ttg att atc cgt cgc gtg atc aac aaa 451Ala Val Leu Gly Leu Ile Gly Leu Ile Ile Arg Arg Val Ile Asn Lys
105 110 115acc gtc ttc ctg tcc acc tca cgc tcc gac aaa gtg atg tat gtg cta 499Thr Val Phe Leu Ser Thr Ser Arg Ser Asp Lys Val Met Tyr Val Leu
120 125 130ctc ggc gct gca att ttg tcc ggt ttc atc gcc acc gtc tcc acc cag 547Leu Gly Ala Ala Ile Leu Ser Gly Phe Ile Ala Thr Val Ser Thr Gln
135 140 145gtc ttc ggc ggc gca cac ggc tac gac tac cgc gaa acc atc tcc cca 595Val Phe Gly Gly Ala His Gly Tyr Asp Tyr Arg Glu Thr Ile Ser Pro150 155 160 165tgg gtg cgc caa ctg ctc atc ttc aac gct caa cca gag ctc atg gct 643Trp Val Arg Gln Leu Leu Ile Phe Asn Ala Gln Pro Glu Leu Met Ala
170 175 180gat gtc cct tgg gaa ttc aag gtc cac atc gtc gct gga ttc acc ctc 691Asp Val Pro Trp Glu Phe Lys Val His Ile Val Ala Gly Phe Thr Leu
185 190 195atc gca ctg tgg cca ttc acc cgc cta gtc cac gcg ttc tcc gca cca 739Ile Ala Leu Trp Pro Phe Thr Arg Leu Val His Ala Phe Ser Ala Pro
200 205 210gtt gga tac gtc acc cgc ccc tac gtg gtc tat cgc acc cgc gac acc 787Val Gly Tyr Val Thr Arg Pro Tyr Val Val Tyr Arg Thr Arg Asp Thr
215 220 225acc tct gaa ccg gca cgc caa aac gtc gcc tgg gaa ccg atc cgc tcg 835Thr Ser Glu Pro Ala Arg Gln Asn Val Ala Trp Glu Pro Ile Arg Ser230 235 240 245gtc aaa aat cag ctc gac aat gac tcg aaa tgg cac ggc gcc 877Val Lys Asn Gln Leu Asp Asn Asp Ser Lys Trp His Gly Ala
250 255taaattcctc acaagccccc tag 900<210>292<211>259<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>292Met Ser Asn Phe Glu Thr Phe Leu Trp Val Ala Tyr Pro Trp Leu Cys1 5 10 15Ile Ala Ala Tyr Ile Ile Gly Ile Ser Trp Arg Trp Arg Ala Asp Gln
20 25 30Phe Gly Trp Thr Thr His Ser Ser Gln Ile Tyr Glu Ser Lys Leu Leu
35 40 45Arg Ile Ala Ser Pro Leu Phe His Trp Gly Met Val Phe Val Val Ile
50 55 60Gly His Leu Met Gly Leu Ala Ile Pro Lys Ser Trp Thr Gln Ala Val65 70 75 80Gly Ile Ser Asp Ala Ala Tyr His Leu Ile Ala Thr Ile Pro Gly Thr
85 90 95Ile Ala Gly Ile Ala Ala Val Leu Gly Leu Ile Gly Leu Ile Ile Arg
100 105 110Arg Val Ile Asn Lys Thr Val Phe Leu Ser Thr Ser Arg Ser Asp Lys
115 120 125Val Met Tyr Val Leu Leu Gly Ala Ala Ile Leu Ser Gly Phe Ile Ala
130 135 140Thr Val Ser Thr Gln Val Phe Gly Gly Ala His Gly Tyr Asp Tyr Arg145 150 155 160Glu Thr Ile Ser Pro Trp Val Arg Gln Leu Leu Ile Phe Asn Ala Gln
165 170 175Pro Glu Leu Met Ala Asp Val Pro Trp Glu Phe Lys Val His Ile Val
180 185 190Ala Gly Phe Thr Leu Ile Ala Leu Trp Pro Phe Thr Arg Leu Val His
195 200 205Ala Phe Ser Ala Pro Val Gly Tyr Val Thr Arg Pro Tyr Val Val Tyr
210 215 220Arg Thr Arg Asp Thr Thr Ser Glu Pro Ala Arg Gln Asn Val Ala Trp225 230 235 240Glu Pro Ile Arg Ser Val Lys Asn Gln Leu Asp Asn Asp Ser Lys Trp
245 250 255His Gly Ala<210>293<211>813<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(790)<223>RXA00471<400>293acgcatcaaa tctgaaatcg gcgaaggcac cgaagtggca atcaccatgg atgtgtagtt 60ggtcgtacgc gcgtgtcttc ggggctgtaa cctgaaaggc atg gtt gat gtg ttt 115
Met Val Asp Val Phe
1 5ttg gtc gat gac cac tcc gtg ttt cgc tcc ggc gtc aaa gca gaa cta 163Leu Val Asp Asp His Ser Val Phe Arg Ser Gly Val Lys Ala Glu Leu
10 15 20ggc aac gcc gtc aca gta gtc ggc gaa gca ggg acg gtg gcc gac gcc 211Gly Asn Ala Val Thr Val Val Gly Glu Ala Gly Thr Val Ala Asp Ala
25 30 35gta gcc ggc atc aag gca agc aaa cca gag gta gtg ctt ctc gac gtc 259Val Ala Gly Ile Lys Ala Ser Lys Pro Glu Val Val Leu Leu Asp Val
40 45 50cac atg ccc gac ggc ggc ggc ctc gca gtg ctc cag cag atc aac gac 307His Met Pro Asp Gly Gly Gly Leu Ala Val Leu Gln Gln Ile Asn Asp
55 60 65tcc gat gtg gac acc att ttc ttg gca ctc agt gtc tct gat gct gcg 355Ser Asp Val Asp Thr Ile Phe Leu Ala Leu Ser Val Ser Asp Ala Ala70 75 80 85gaa gat gtc atc gcc atc atc cgt ggc ggt gcc agg gga tac gtg acc 403Glu Asp Val Ile Ala Ile Ile Arg Gly Gly Ala Arg Gly Tyr Val Thr
90 95 100aaa tca atc tcc ggt gaa gaa ctc atc gaa gcc atc aac cgc gtg aaa 451Lys Ser Ile Ser Gly Glu Glu Leu Ile Glu Ala Ile Asn Arg Val Lys
105 110 115tcc ggc gac gca ttc ttc tca cca cgc ctg gca ggc ttt gtc ctc gac 499Ser Gly Asp Ala Phe Phe Ser Pro Arg Leu Ala Gly Phe Val Leu Asp
120 125 130gcc ttc gcc gcc ccc gat tcc gca gct ggc gca ggc att gtc gac gca 547Ala Phe Ala Ala Pro Asp Ser Ala Ala Gly Ala Gly Ile Val Asp Ala
135 140 145ccc gaa aaa gac gcc gcc gta gaa tcc gga aaa atc ctc gac gac cca 595Pro Glu Lys Asp Ala Ala Val Glu Ser Gly Lys Ile Leu Asp Asp Pro150 155 160 165gtt gtc gac gcc ctc acc cgc cgc gaa ctc gaa gtc ctc cgc cta cta 643Val Val Asp Ala Leu Thr Arg Arg Glu Leu Glu Val Leu Arg Leu Leu
170 175 180gcc cgc ggc tac acc tac aaa gaa atc ggc aaa gaa ctg ttc att tcc 691Ala Arg Gly Tyr Thr Tyr Lys Glu Ile Gly Lys Glu Leu Phe Ile Ser
185 190 195gtc aaa acc gtg gaa acc cac gcc tca aac att ctg cgg aaa acc caa 739Val Lys Thr Val Glu Thr His Ala Ser Asn Ile Leu Arg Lys Thr Gln
200 205 210caa tcc aac cgc cac gcg ttg acc cgg tgg gct cac tcg agg gat ctt 787Gln Ser Asn Arg His Ala Leu Thr Arg Trp Ala His Ser Arg Asp Leu
215 220 225gac taa tggcggc taaaaagagt ggc 813Asp230<210>294<211>230<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>294Met Val Asp Val Phe Leu Val Asp Asp His Ser Val Phe Arg Ser Gly1 5 10 15Val Lys Ala Glu Leu Gly Asn Ala Val Thr Val Val Gly Glu Ala Gly
20 25 30Thr Val Ala Asp Ala Val Ala Gly Ile Lys Ala Ser Lys Pro Glu Val
35 40 45Val Leu Leu Asp Val His Met Pro Asp Gly Gly Gly Leu Ala Val Leu
50 55 60Gln Gln Ile Asn Asp Ser Asp Val Asp Thr Ile Phe Leu Ala Leu Ser65 70 75 80Val Ser Asp Ala Ala Glu Asp Val Ile Ala Ile Ile Arg Gly Gly Ala
85 90 95Arg Gly Tyr Val Thr Lys Ser Ile Ser Gly Glu Glu Leu Ile Glu Ala
100 105 110Ile Asn Arg Val Lys Ser Gly Asp Ala Phe Phe Ser Pro Arg Leu Ala
115 120 125Gly Phe Val Leu Asp Ala Phe Ala Ala Pro Asp Ser Ala Ala Gly Ala
130 135 140Gly Ile Val Asp Ala Pro Glu Lys Asp Ala Ala Val Glu Ser Gly Lys145 150 155 160Ile Leu Asp Asp Pro Val Val Asp Ala Leu Thr Arg Arg Glu Leu Glu
165 170 175Val Leu Arg Leu Leu Ala Arg Gly Tyr Thr Tyr Lys Glu Ile Gly Lys
180 185 190Glu Leu Phe Ile Ser Val Lys Thr Val Glu Thr His Ala Ser Asn Ile
195 200 205Leu Arg Lys Thr Gln Gln Ser Asn Arg His Ala Leu Thr Arg Trp Ala
210 215 220His Ser Arg Asp Leu Asp225 230<210>295<211>936<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(913)<223>RXA00133<400>295gttacatcag atgaggatgc cctatgggtg tacacatgcg acgggtgtat tgcaggagga 60aatttgaagg tggataccca gcggattaaa gatgatgaag atg cta ttc gtt cgg 115
Met Leu Phe Val Arg
1 5cgg ctg aca tcg ctg aaa acc gca aca ggc atc cca gtc acc atg ttc 163Arg Leu Thr Ser Leu Lys Thr Ala Thr Gly Ile Pro Val Thr Met Phe
10 15 20gcc act gtg ttg cag gac aat cgc ctg caa att act cag tgg gtt ggg 211Ala Thr Val Leu Gln Asp Asn Arg Leu Gln Ile Thr Gln Trp Val Gly
25 30 35ttg cgt acc ccg gct ctg cag aat ctg gtc att gaa cca ggt gtg ggc 259Leu Arg Thr Pro Ala Leu Gln Asn Leu Val Ile Glu Pro Gly Val Gly
40 45 50gtt ggt gga cgc gtc gtc gca acc cgt cgt ccg gtt ggt gtg agt gat 307Val Gly Gly Arg Val Val Ala Thr Arg Arg Pro Val Gly Val Ser Asp
55 60 65tac acc agg gca aat gtc att tca cat gag aag gat tcc gcg att cag 355Tyr Thr Arg Ala Asn Val Ile Ser His Glu Lys Asp Ser Ala Ile Gln70 75 80 85gat gag ggc ctt cat tcc att gtc gca gtt ccc gtg atc gtg cac cgc 403Asp Glu Gly Leu His Ser Ile Val Ala Val Pro Val Ile Val His Arg
90 95 100gaa att cgt ggc gtt ttg tat gtt ggc gtt cac tct gcg gtg cgt ctc 451Glu Ile Arg Gly Val Leu Tyr Val Gly Val His Ser Ala Val Arg Leu
105 110 115ggc gac act gtt att gaa gaa gtc acc atg act gcg cgc acg ttg gaa 499Gly Asp Thr Val Ile Glu Glu Val Thr Met Thr Ala Arg Thr Leu Glu
120 125 130caa aac ctg gcg atc aac tcc gcg ctt cgc cgc aat ggc gtt cct gat 547Gln Asn Leu Ala Ile Asn Ser Ala Leu Arg Arg Asn Gly Val Pro Asp
135 140 145ggt cgc ggt tcc ctc aaa gct aac cgc gtg atg aat ggg gcg gag tgg 595Gly Arg Gly Ser Leu Lys Ala Asn Arg Val Met Asn Gly Ala Glu Trp150 155 160 165gag cag gtt cgt tcc act cat tcc aag ctg cgc atg ctg gca aat cgt 643Glu Gln Val Arg Ser Thr His Ser Lys Leu Arg Met Leu Ala Asn Arg
170 175 180gtg acc gat gag gat ctg cgc cgc gat ttg gaa gag ctt tgc gat cag 691Val Thr Asp Glu Asp Leu Arg Arg Asp Leu Glu Glu Leu Cys Asp Gln
185 190 195atg gtc acc cca gtc cgc atc aag cag acc acc aag ctg tcc gcg cgt 739Met Val Thr Pro Val Arg Ile Lys Gln Thr Thr Lys Leu Ser Ala Arg
200 205 210gag ttg gac gtg ctg gct tgt gtc gcg ctc ggt cac acc aac gtc gaa 787Glu Leu Asp Val Leu Ala Cys Val Ala Leu Gly His Thr Asn Val Glu
215 220 225gct gct gaa gag atg ggc atc ggc gcg gaa acc gtc aag agc tac ctg 835Ala Ala Glu Glu Met Gly Ile Gly Ala Glu Thr Val Lys Ser Tyr Leu230 235 240 245cgc tcg gtc atg cgc aag ctc ggc gcc cac acg cgc tac gag gca gtc 883Arg Ser Val Met Arg Lys Leu Gly Ala His Thr Arg Tyr Glu Ala Val
250 255 260aac gca gca cgc cgg atc ggc gca ctg cct taaaaagatt ttgctttacg 933Asn Ala Ala Arg Arg Ile Gly Ala Leu Pro
265 270acg 936<210>296<211>271<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400> 296Met Leu Phe Val Arg Arg Leu Thr Ser Leu Lys Thr Ala Thr Gly Ile1 5 10 15Pro Val Thr Met Phe Ala Thr Val Leu Gln Asp Asn Arg Leu Gln Ile
20 25 30Thr Gln Trp Val Gly Leu Arg Thr Pro Ala Leu Gln Asn Leu Val Ile
35 40 45Glu Pro Gly Val Gly Val Gly Gly Arg Val Val Ala Thr Arg Arg Pro
50 55 60Val Gly Val Ser Asp Tyr Thr Arg Ala Asn Val Ile Ser His Glu Lys65 70 75 80Asp Ser Ala Ile Gln Asp Glu Gly Leu His Ser Ile Val Ala Val Pro
85 90 95Val Ile Val His Arg Glu Ile Arg Gly Val Leu Tyr Val Gly Val His
100 105 110Ser Ala Val Arg Leu Gly Asp Thr Val Ile Glu Glu Val Thr Met Thr
115 120 125Ala Arg Thr Leu Glu Gln Asn Leu Ala Ile Asn Ser Ala Leu Arg Arg
130 135 140Asn Gly Val Pro Asp Gly Arg Gly Ser Leu Lys Ala Asn Arg Val Met145 150 155 160Asn Gly Ala Glu Trp Glu Gln Val Arg Ser Thr His Ser Lys Leu Arg
165 170 175Met Leu Ala Asn Arg Val Thr Asp Glu Asp Leu Arg Arg Asp Leu Glu
180 185 190Glu Leu Cys Asp Gln Met Val Thr Pro Val Arg Ile Lys Gln Thr Thr
195 200 205Lys Leu Ser Ala Arg Glu Leu Asp Val Leu Ala Cys Val Ala Leu Gly
210 215 220His Thr Asn Val Glu Ala Ala Glu Glu Met Gly Ile Gly Ala Glu Thr225 230 235 240Val Lys Ser Tyr Leu Arg Ser Val Met Arg Lys Leu Gly Ala His Thr
245 250 255Arg Tyr Glu Ala Val Asn Ala Ala Arg Arg Ile Gly Ala Leu Pro
260 265 270<210>297<211>759<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (736)<223>RXA00650<400>297aaggctagac taaagtacga ttcatctgct catcgatact cttgaaggcg cattttcatt 60cgaaacgaag tgcgccattg ggaaggacct agttcaaaca atg att cgc gtg ctg 115
Met Ile Arg Val Leu
1 5ctt gct gat gac cac gaa atc gtg agg ctc gga ctc cga gct gtg ctg 163Leu Ala Asp Asp His Glu Ile Val Arg Leu Gly Leu Arg Ala Val Leu
10 15 20gaa agc gcc gag gac att gaa gtg gtg ggc gaa gtc tcc acc gcc gaa 211Glu Ser Ala Glu Asp Ile Glu Val Val Gly Glu Val Ser Thr Ala Glu
25 30 35ggt gcg gtg cag gca gcc caa gaa ggc gga atc gac gtc atc ttg atg 259Gly Ala Val Gln Ala Ala Gln Glu Gly Gly Ile Asp Val Ile Leu Met
40 45 50gac ctc cga ttc ggc ccc ggc gtc caa gga acc cag gtt tcc aca ggc 307Asp Leu Arg Phe Gly Pro Gly Val Gln Gly Thr Gln Val Ser Thr Gly
55 60 65gca gac gcc acc gca gcc atc aag cga aac atc gat aac ccg cca aaa 355Ala Asp Ala Thr Ala Ala Ile Lys Arg Asn Ile Asp Asn Pro Pro Lys70 75 80 85gtc ctg gtc gtg acc aac tac gac acc gac aca gac atc ctc ggc gca 403Val Leu Val Val Thr Asn Tyr Asp Thr Asp Thr Asp Ile Leu Gly Ala
90 95 100atc gaa gcc ggc gca ctg ggc tac ctg ctc aaa gac gcc cca ccg agc 451Ile Glu Ala Gly Ala Leu Gly Tyr Leu Leu Lys Asp Ala Pro Pro Ser
105 110 115gaa ctc ctg gca gca gta cga tcc gca gca gaa ggt gac tcc aca ctg 499Glu Leu Leu Ala Ala Val Arg Ser Ala Ala Glu Gly Asp Ser Thr Leu
120 125 130tca ccc atg gtt gcg aac cgc ctg atg act cgc gtg cgc acc ccc aaa 547Ser Pro Met Val Ala Asn Arg Leu Met Thr Arg Val Arg Thr Pro Lys
135 140 145acc tca ctc acc cca cgt gaa ctg gaa gtt ctc aag ctg gtt gcc ggt 595Thr Ser Leu Thr Pro Arg Glu Leu Glu Val Leu Lys Leu Val Ala Gly150 155 160 165gga tcc tcc aac cgc gac att ggc cgt atc ctc ttc ctc tca gaa gcc 643Gly Ser Ser Asn Arg Asp Ile Gly Arg Ile Leu Phe Leu Ser Glu Ala
170 175 180acg gtg aaa tcc cac ctc gtg cac atc tac gac aag ctc ggc gtg cgg 691Thr Val Lys Ser His Leu Val His Ile Tyr Asp Lys Leu Gly Val Arg
185 190 195tca cgt acc tcc gct gtc gca gcc gca cgt gag cag ggg ctg ctg 736Ser Arg Thr Ser Ala Val Ala Ala Ala Arg Glu Gln Gly Leu Leu
200 205 210tagcgggggt tgctgcaagg ctt 759<210>298<211>212<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>298Met Ile Arg Val Leu Leu Ala Asp Asp His Glu Ile Val Arg Leu Gly1 5 10 15Leu Arg Ala Val Leu Glu Ser Ala Glu Asp Ile Glu Val Val Gly Glu
20 25 30Val Ser Thr Ala Glu Gly Ala Val Gln Ala Ala Gln Glu Gly Gly Ile
35 40 45Asp Val Ile Leu Met Asp Leu Arg Phe Gly Pro Gly Val Gln Gly Thr
50 55 60Gln Val Ser Thr Gly Ala Asp Ala Thr Ala Ala Ile Lys Arg Asn Ile65 70 75 80Asp Asn Pro Pro Lys Val Leu Val Val Thr Asn Tyr Asp Thr Asp Thr
85 90 95Asp Ile Leu Gly Ala Ile Glu Ala Gly Ala Leu Gly Tyr Leu Leu Lys
100 105 110Asp Ala Pro Pro Ser Glu Leu Leu Ala Ala Val Arg Ser Ala Ala Glu
115 120 125Gly Asp Ser Thr Leu Ser Pro Met Val Ala Asn Arg Leu Met Thr Arg
130 135 140Val Arg Thr Pro Lys Thr Ser Leu Thr Pro Arg Glu Leu Glu Val Leu145 150 155 160Lys Leu Val Ala Gly Gly Ser Ser Asn Arg Asp Ile Gly Arg Ile Leu
165 170 175Phe Leu Ser Glu Ala Thr Val Lys Ser His Leu Val His Ile Tyr Asp
180 185 190Lys Leu Gly Val Arg Ser Arg Thr Ser Ala Val Ala Ala Ala Arg Glu
195 200 205Gln Gly Leu Leu
210<210>299<211>655<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(24)..(632)<223>RXA01189<400>299cacctgcaga aaaggaagct taa atg att tcc att tcc atc gcc gac gac gaa 53
Met Ile Ser Ile Ser Ile Ala Asp Asp Glu
1 5 10gcc ctg atc gca agc tcc ctg gca acc ttg ctc agc ttg gaa ccc gat 101Ala Leu Ile Ala Ser Ser Leu Ala Thr Leu Leu Ser Leu Glu Pro Asp
15 20 25tta gac gtc cga cct acc gca gga tcc ggt gaa gaa ctc att gaa acg 149Leu Asp Val Arg Pro Thr Ala Gly Ser Gly Glu Glu Leu Ile Glu Thr
30 35 40tgg gcg gat cca agc aac cga acc gat gta tgc gtc ctt gac ctt caa 197Trp Ala Asp Pro Ser Asn Arg Thr Asp Val Cys Val Leu Asp Leu Gln
45 50 55ctc gga ggc atc gac ggc atc gac acc gcc acc cgg ctc atg gaa acc 245Leu Gly Gly Ile Asp Gly Ile Asp Thr Ala Thr Arg Leu Met Glu Thr
60 65 70acc cca gat ttg gcc gtg ctc atc gtg acc agc cac gcc agg ccc cga 293Thr Pro Asp Leu Ala Val Leu Ile Val Thr Ser His Ala Arg Pro Arg75 80 85 90caa ctc aaa cgc gcg ctt gca gca ggt gtt tta gga ttc ttg ccc aaa 341Gln Leu Lys Arg Ala Leu Ala Ala Gly Val Leu Gly Phe Leu Pro Lys
95 100 105aca tcc acc gca gat gaa ttc gcc acc gca atc cgc acc gtt cac gct 389Thr Ser Thr Ala Asp Glu Phe Ala Thr Ala Ile Arg Thr Val His Ala
110 115 120gga cga cgc tac atc gac ccc gaa cta gcc gcc atg acg atc agc gcc 437Gly Arg Arg Tyr Ile Asp Pro Glu Leu Ala Ala Met Thr Ile Ser Ala
125 130 135ggt gaa tcc cca tta acc aac cgt gaa gaa gaa gtc ctc gaa cta gca 485Gly Glu Ser Pro Leu Thr Asn Arg Glu Glu Glu Val Leu Glu Leu Ala
140 145 150ggc caa gga cta agc gcc gaa gaa att gcg gtg gca gcg cac ctc gcg 533Gly Gln Gly Leu Ser Ala Glu Glu Ile Ala Val Ala Ala His Leu Ala155 160 165 170ccg gga acc acc cgc aac tat tta tcc caa gct atg aca aaa gta ggc 581Pro Gly Thr Thr Arg Asn Tyr Leu Ser Gln Ala Met Thr Lys Val Gly
175 180 185gcg cag aat cgc ttt gaa gcg ttc acg cgc gcc agg gaa ttg ggc tgg 629Ala Gln Asn Arg Phe Glu Ala Phe Thr Arg Ala Arg Glu Leu Gly Trp
190 195 200ttg tagcttgtgg cttatctcct att 655Leu<210>300<211>203<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>300Met Ile Ser Ile Ser Ile Ala Asp Asp Glu Ala Leu Ile Ala Ser Ser1 5 10 15Leu Ala Thr Leu Leu Ser Leu Glu Pro Asp Leu Asp Val Arg Pro Thr
20 25 30Ala Gly Ser Gly Glu Glu Leu Ile Glu Thr Trp Ala Asp Pro Ser Asn
35 40 45Arg Thr Asp Val Cys Val Leu Asp Leu Gln Leu Gly Gly Ile Asp Gly
50 55 60Ile Asp Thr Ala Thr Arg Leu Met Glu Thr Thr Pro Asp Leu Ala Val65 70 75 80Leu Ile Val Thr Ser His Ala Arg Pro Arg Gln Leu Lys Arg Ala Leu
85 90 95Ala Ala Gly Val Leu Gly Phe Leu Pro Lys Thr Ser Thr Ala Asp Glu
100 105 110Phe Ala Thr Ala Ile Arg Thr Val His Ala Gly Arg Arg Tyr Ile Asp
115 120 125Pro Glu Leu Ala Ala Met Thr Ile Ser Ala Gly Glu Ser Pro Leu Thr
130 135 140Asn Arg Glu Glu Glu Val Leu Glu Leu Ala Gly Gln Gly Leu Ser Ala145 150 155 160Glu Glu Ile Ala Val Ala Ala His Leu Ala Pro Gly Thr Thr Arg Asn
165 170 175Tyr Leu Ser Gln Ala Met Thr Lys Val Gly Ala Gln Asn Arg Phe Glu
180 185 190Ala Phe Thr Arg Ala Arg Glu Leu Gly Trp Leu
195 200<210>301<211>753<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (730)<223>RXA01607<400>301gggctgaagg gctgggcgga acaataatta ttgaatctac aatcggatcg ggaactggaa 60tttccgcccg ttttccctat ccacaaaagg accaagataa gtg atc cgt att ctg 115
Val Ile Arg Ile Leu
1 5ttg gct gat gat cat ccc gtt gtt cgc gca ggc ctt gcc tcc ttg ctg 163Leu Ala Asp Asp His Pro Val Val Arg Ala Gly Leu Ala Ser Leu Leu
10 15 20gtg agt gaa gat gat ttt gag ata gtg gac atg gtg ggc acc cca gat 211Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met Val Gly Thr Pro Asp
25 30 35gat gcc gtt gcg cgc gcc gcg gaa ggc ggg gtg gat gtg gtg ttg atg 259Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val Asp Val Val Leu Met
40 45 50gat ctg cgt ttt ggt gat caa cca ggc atc gag gtc gcc ggc ggg gta 307Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu Val Ala Gly Gly Val
55 60 65gag gca acg cgt cgc atc cgt gcg ctg gac aac ccg cca cag gta ctg 355Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn Pro Pro Gln Val Leu70 75 80 85gtg gtg acc aac tac tcc aca gac ggc gat gtg gtg ggc gca gta tct 403Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val Val Gly Ala Val Ser
90 95 100gct ggt gcc gtg ggg tat ttg ctc aaa gat agc tcc cca gaa gat ctc 451Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser Ser Pro Glu Asp Leu
105 110 115att gcc ggt gtt cgc gat gcc gcg cgg gga gaa tca gtg ctt tca aag 499Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu Ser Val Leu Ser Lys
120 125 130cag gtc gcc agc aag atc atg ggg cgg atg aac aac ccc atg act gct 547Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn Asn Pro Met Thr Ala
135 140 145ctc agt gcc aga gaa att gaa gtg ctg tcc ttg gtg gcg caa ggg caa 595Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu Val Ala Gln Gly Gln150 155 160 165agc aat aga gaa atc ggc aag aaa ctt ttc ctc act gag gcc acg gtg 643Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu Thr Glu Ala Thr Val
170 175 180aaa agt cac atg ggg cat gtg ttc aac aag ctg gat gtc acc tct aga 691Lys Ser His Met Gly His Val Phe Asn Lys Leu Asp Val Thr Ser Arg
185 190 195aca gct gcg gta gct gaa gcc aga cag cgc gga att atc tagacgcaca 740Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly Ile Ile
200 205 210cgtgttggta acc 753<210>302<211>210<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>302Val Ile Arg Ile Leu Leu Ala Asp Asp His Pro Val Val Arg Ala Gly1 5 10 15Leu Ala Ser Leu Leu Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met
20 25 30Val Gly Thr Pro Asp Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val
35 40 45Asp Val Val Leu Met Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu
50 55 60Val Ala Gly Gly Val Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn65 70 75 80Pro Pro Gln Val Leu Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val
85 90 95Val Gly Ala Val Ser Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser
100 105 110Ser Pro Glu Asp Leu Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu
115 120 125Ser Val Leu Ser Lys Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn
130 135 140Asn Pro Met Thr Ala Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu145 150 155 160Val Ala Gln Gly Gln Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu
165 170 175Thr Glu Ala Thr Val Lys Ser His Met Gly His Val Phe Asn Lys Leu
180 185 190Asp Val Thr Ser Arg Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly
195 200 205Ile Ile
210<210>303<211>1392<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (1369)<223>RXN00470<400>303tcataaccag gttgggcaaa agggatgaat ccctggttgt ggtggggctc ctgaaaagta 60ctcatagact ctattgtgga gtgttgaggc tgataagtga atg ggg gaa agc cct 115
Met Gly Glu Ser Pro
1 5gaa aag gtg gcg ttc agg gtc ttc cct gat ggt ttg gtg tcg cag ggg 163Glu Lys Val Ala Phe Arg Val Phe Pro Asp Gly Leu Val Ser Gln Gly
10 15 20cat gac atg atc gaa gat atg agt aac aca cct gcg cct tat acc ccg 211His Asp Met Ile Glu Asp Met Ser Asn Thr Pro Ala Pro Tyr Thr Pro
25 30 35cag cct gcg ggg caa gcg gtg cct tta tat ccc acg ttt acc cgg tca 259Gln Pro Ala Gly Gln Ala Val Pro Leu Tyr Pro Thr Phe Thr Arg Ser
40 45 50aga gat ggt cgg gtt gtt gcg ggt gtc gca tcg ggg ctg gca aag cat 307Arg Asp Gly Arg Val Val Ala Gly Val Ala Ser Gly Leu Ala Lys His
55 60 65ctt aat gtg tcg gtg ttt tgg gtt cgt gcg ctg ctg att ttt gcg gcg 355Leu Asn Val Ser Val Phe Trp Val Arg Ala Leu Leu Ile Phe Ala Ala70 75 80 85ttg ctg agc ggt gcg ggt ctt ttt gcg tat gcc ttg att tgg att ttt 403Leu Leu Ser Gly Ala Gly Leu Phe Ala Tyr Ala Leu Ile Trp Ile Phe
90 95 100acg cgc att gag aaa aag ggg agt ggg gag gcg tcg aca agc aag cgc 451Thr Arg Ile Glu Lys Lys Gly Ser Gly Glu Ala Ser Thr Ser Lys Arg
105 110 115tgg gtg tcg tgg tgc ctg gtg ctg ctc gct atc ggt ggt gct gcg gcg 499Trp Val Ser Trp Cys Leu Val Leu Leu Ala Ile Gly Gly Ala Ala Ala
120 125 130tcg gtg atg ctg agc acc ggc ttc gcg gtg ggc acg ttg gtg ccc atc 547Ser Val Met Leu Ser Thr Gly Phe Ala Val Gly Thr Leu Val Pro Ile
135 140 145ggc gtg gtc ggt gtg ggc ctg ttg atg gtg tgg ctg gcg tat gac cgc 595Gly Val Val Gly Val Gly Leu Leu Met Val Trp Leu Ala Tyr Asp Arg150 155 160 165ggg gtg gaa tcc ggc ccg aat ctg ctg att att gcc acc ggc ggt gtg 643Gly Val Glu Ser Gly Pro Asn Leu Leu Ile Ile Ala Thr Gly Gly Val
170 175 180ttg atg ctg gtg gcg atc gtg ctg atc gtg atg aat tgg aac acc cag 691Leu Met Leu Val Ala Ile Val Leu Ile Val Met Asn Trp Asn Thr Gln
185 190 195gac ggc ttc gtc atg gcg ctg gtg gcc gtg gtg ctc acg ctg gtg ggt 739Asp Gly Phe Val Met Ala Leu Val Ala Val Val Leu Thr Leu Val Gly
200 205 210gtg gct gcg ctg ggc gtt ccg ctg tgg gtg cgg atg tgg gat cag ctg 787Val Ala Ala Leu Gly Val Pro Leu Trp Val Arg Met Trp Asp Gln Leu
215 220 225ggc gag gag cgc gcg gaa aaa gcc gca gct gct gag cgc gca gat att 835Gly Glu Glu Arg Ala Glu Lys Ala Ala Ala Ala Glu Arg Ala Asp Ile230 235 240 245gct tcc cgc ctg cat gat tcg gta ctg cag acc ttg gcg ctg att caa 883Ala Ser Arg Leu His Asp Ser Val Leu Gln Thr Leu Ala Leu Ile Gln
250 255 260aag cgt gcc gac gac ccc gcc gaa gtc gcc cgc ctg gcc cgc ggg cag 931Lys Arg Ala Asp Asp Pro Ala Glu Val Ala Arg Leu Ala Arg Gly Gln
265 270 275gaa cgc gag ctg cgt caa tgg ctg ttt gat tcc caa gat aaa aca cct 979Glu Arg Glu Leu Arg Gln Trp Leu Phe Asp Ser Gln Asp Lys Thr Pro
280 285 290caa aca acc ggc act gtc ttt act gcg ttg gag cgc gcc tgc ggt gaa 1027Gln Thr Thr Gly Thr Val Phe Thr Ala Leu Glu Arg Ala Cys Gly Glu
295 300 305gtc gag gat att tac gct ctg cgt atc gtg cct gtg acc gtg gga acc 1075Val Glu Asp lle Tyr Ala Leu Arg Ile Val Pro Val Thr Val Gly Thr310 315 320 325gat gaa gcg ctg act gag aaa acg cag gca gcg gtg atg gca gtc cgc 1123Asp Glu Ala Leu Thr Glu Lys Thr Gln Ala Ala Val Met Ala Val Arg
330 335 340gaa gca ctc gtg aac gtg gcc aag cat gcc ggc gtg gaa acc gcc gat 1171Glu Ala Leu Val Asn Val Ala Lys His Ala Gly Val Glu Thr Ala Asp
345 350 355gtg tac gcc gaa att atg ctc ggc gaa ctg aac att ttc gtc cgc gac 1219Val Tyr Ala Glu Ile Met Leu Gly Glu Leu Asn Ile Phe Val Arg Asp
360 365 370cgc ggt gca gga ttc gac ccc gac aac atc ccc gac ggg cac cac ggg 1267Arg Gly Ala Gly Phe Asp Pro Asp Asn Ile Pro Asp Gly His His Gly
375 380 385ctc gcc gaa tcc gtc caa ggc cgc gtc gaa cga gcc ggc gga aaa gta 1315Leu Ala Glu Ser Val Gln Gly Arg Val Glu Arg Ala Gly Gly Lys Val390 395 400 405cgc atc aaa tct gaa atc ggc gaa ggc acc gaa gtg gca atc acc atg 1363Arg Ile Lys Ser Glu Ile Gly Glu Gly Thr Glu Val Ala Ile Thr Met
410 415 420gat gtg tagttggtcg tacgcgcgtg tct 1392Asp Val<210>304<211>423<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>304Met Gly Glu Ser Pro Glu Lys Val Ala Phe Arg Val Phe Pro Asp Gly1 5 10 15Leu Val Ser Gln Gly His Asp Met Ile Glu Asp Met Ser Asn Thr Pro
20 25 30Ala Pro Tyr Thr Pro Gln Pro Ala Gly Gln Ala Val Pro Leu Tyr Pro
35 40 45Thr Phe Thr Arg Ser Arg Asp Gly Arg Val Val Ala Gly Val Ala Ser
50 55 60Gly Leu Ala Lys His Leu Asn Val Ser Val Phe Trp Val Arg Ala Leu65 70 75 80Leu Ile Phe Ala Ala Leu Leu Ser Gly Ala Gly Leu Phe Ala Tyr Ala
85 90 95Leu Ile Trp Ile Phe Thr Arg Ile Glu Lys Lys Gly Ser Gly Glu Ala
100 105 110Ser Thr Ser Lys Arg Trp Val Ser Trp Cys Leu Val Leu Leu Ala Ile
115 120 125Gly Gly Ala Ala Ala Ser Val Met Leu Ser Thr Gly Phe Ala Val Gly
130 135 140Thr Leu Val Pro Ile Gly Val Val Gly Val Gly Leu Leu Met Val Trp145 150 155 160Leu Ala Tyr Asp Arg Gly Val Glu Ser Gly Pro Asn Leu Leu Ile Ile
165 170 175Ala Thr Gly Gly Val Leu Met Leu Val Ala Ile Val Leu Ile Val Met
180 185 190Asn Trp Asn Thr Gln Asp Gly Phe Val Met Ala Leu Val Ala Val Val
195 200 205Leu Thr Leu Val Gly Val Ala Ala Leu Gly Val Pro Leu Trp Val Arg
210 215 220Met Trp Asp Gln Leu Gly Glu Glu Arg Ala Glu Lys Ala Ala Ala Ala225 230 235 240Glu Arg Ala Asp Ile Ala Ser Arg Leu His Asp Ser Val Leu Gln Thr
245 250 255Leu Ala Leu Ile Gln Lys Arg Ala Asp Asp Pro Ala Glu Val Ala Arg
260 265 270Leu Ala Arg Gly Gln Glu Arg Glu Leu Arg Gln Trp Leu Phe Asp Ser
275 280 285Gln Asp Lys Thr Pro Gln Thr Thr Gly Thr Val Phe Thr Ala Leu Glu
290 295 300Arg Ala Cys Gly Glu Val Glu Asp Ile Tyr Ala Leu Arg Ile Val Pro305 310 315 320Val Thr Val Gly Thr Asp Glu Ala Leu Thr Glu Lys Thr Gln Ala Ala
325 330 335Val Met Ala Val Arg Glu Ala Leu Val Asn Val Ala Lys His Ala Gly
340 345 350Val Glu Thr Ala Asp Val Tyr Ala Glu Ile Met Leu Gly Glu Leu Asn
355 360 365Ile Phe Val Arg Asp Arg Gly Ala Gly Phe Asp Pro Asp Asn Ile Pro
370 375 380Asp Gly His His Gly Leu Ala Glu Ser Val Gln Gly Arg Val Glu Arg385 390 395 400Ala Gly Gly Lys Val Arg Ile Lys Ser Glu Ile Gly Glu Gly Thr Glu
405 410 415Val Ala Ile Thr Met Asp Val
420<210>305<211>1323<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1300)<223>FRXA00470<400>305tctattgtgg agtgttgagg ctgataagtg aatgggggaa agccctgaaa aggtggcgtt 60cagggtcttc cctgatggtt tggtgtcgca ggggcatgac atg atc gaa gat atg 115
Met Ile Glu Asp Met
1 5agt aac aca cct gcg cct tat acc ccg cag cct gcg ggg caa gcg gtg 163Ser Asn Thr Pro Ala Pro Tyr Thr Pro Gln Pro Ala Gly Gln Ala Val
10 15 20cct tta tat ccc acg ttt acc cgg tca aga gat ggt cgg gtt gtt gcg 211Pro Leu Tyr Pro Thr Phe Thr Arg Ser Arg Asp Gly Arg Val Val Ala
25 30 35ggt gtc gca tcg ggg ctg gca aag cat ctt aat gtg tcg gtg ttt tgg 259Gly Val Ala Ser Gly Leu Ala Lys His Leu Asn Val Ser Val Phe Trp
40 45 50gtt cgt gcg ctg ctg att ttt gcg gcg ttg ctg agc ggt gcg ggt ctt 307Val Arg Ala Leu Leu Ile Phe Ala Ala Leu Leu Ser Gly Ala Gly Leu
55 60 65ttt gcg tat gcc ttg att tgg att ttt acg cgc att gag aaa aag ggg 355Phe Ala Tyr Ala Leu Ile Trp Ile Phe Thr Arg Ile Glu Lys Lys Gly70 75 80 85agt ggg gag gcg tcg aca agc aag cgc tgg gtg tcg tgg tgc ctg gtg 403Ser Gly Glu Ala Ser Thr Ser Lys Arg Trp Val Ser Trp Cys Leu Val
90 95 100ctg ctc gct atc ggt ggt gct gcg gcg tcg gtg atg ctg agc acc ggc 451Leu Leu Ala Ile Gly Gly Ala Ala Ala Ser Val Met Leu Ser Thr Gly
105 110 115ttc gcg gtg ggc acg ttg gtg ccc atc ggc gtg gtc ggt gtg ggc ctg 499Phe Ala Val Gly Thr Leu Val Pro Ile Gly Val Val Gly Val Gly Leu
120 125 130ttg atg gtg tgg ctg gcg tat gac cgc ggg gtg gaa tcc ggc ccg aat 547Leu Met Val Trp Leu Ala Tyr Asp Arg Gly Val Glu Ser Gly Pro Asn
135 140 145ctg ctg att att gcc acc ggc ggt gtg ttg atg ctg gtg gcg atc gtg 595Leu Leu Ile Ile Ala Thr Gly Gly Val Leu Met Leu Val Ala Ile Val150 155 160 165ctg atc gtg atg aat tgg aac acc cag gac ggc ttc gtc atg gcg ctg 643Leu Ile Val Met Asn Trp Asn Thr Gln Asp Gly Phe Val Met Ala Leu
170 175 180gtg gcc gtg gtg ctc acg ctg gtg ggt gtg gct gcg ctg ggc gtt ccg 691Val Ala Val Val Leu Thr Leu Val Gly Val Ala Ala Leu Gly Val Pro
185 190 195ctg tgg gtg cgg atg tgg gat cag ctg ggc gag gag cgc gcg gaa aaa 739Leu Trp Val Arg Met Trp Asp Gln Leu Gly Glu Glu Arg Ala Glu Lys
200 205 210gcc gca gct gct gag cgc gca gat att gct tcc cgc ctg cat gat tcg 787Ala Ala Ala Ala Glu Arg Ala Asp Ile Ala Ser Arg Leu His Asp Ser
215 220 225gta ctg cag acc ttg gcg ctg att caa aag cgt gcc gac gac ccc gcc 835Val Leu Gln Thr Leu Ala Leu Ile Gln Lys Arg Ala Asp Asp Pro Ala230 235 240 245gaa gtc gcc cgc ctg gcc cgc ggg cag gaa cgc gag ctg cgt caa tgg 883Glu Val Ala Arg Leu Ala Arg Gly Gln Glu Arg Glu Leu Arg Gln Trp
250 255 260ctg ttt gat tcc caa gat aaa aca cct caa aca acc ggc act gtc ttt 931Leu Phe Asp Ser Gln Asp Lys Thr Pro Gln Thr Thr Gly Thr Val Phe
265 270 275act gcg ttg gag cgc gcc tgc ggt gaa gtc gag gat att tac gct ctg 979Thr Ala Leu Glu Arg Ala Cys Gly Glu Val Glu Asp Ile Tyr Ala Leu
280 285 290cgt atc gtg cct gtg acc gtg gga acc gat gaa gcg ctg act gag aaa 1027Arg Ile Val Pro Val Thr Val Gly Thr Asp Glu Ala Leu Thr Glu Lys
295 300 305acg cag gca gcg gtg atg gca gtc cgc gaa gca ctc gtg aac gtg gcc 1075Thr Gln Ala Ala Val Met Ala Val Arg Glu Ala Leu Val Asn Val Ala310 315 320 325aag cat gcc ggc gtg gaa acc gcc gat gtg tac gcc gaa att atg ctc 1123Lys His Ala Gly Val Glu Thr Ala Asp Val Tyr Ala Glu Ile Met Leu
330 335 340ggc gaa ctg aac att ttc gtc cgc gac cgc ggt gca gga ttc gac ccc 1171Gly Glu Leu Asn Ile Phe Val Arg Asp Arg Gly Ala Gly Phe Asp Pro
345 350 355gac aac atc ccc gac ggg cac cac ggg ctc gcc gaa tcc gtc caa ggc 1219Asp Asn Ile Pro Asp Gly His His Gly Leu Ala Glu Ser Val Gln Gly
360 365 370cgc gtc gaa cga gcc ggc gga aaa gta cgc atc aaa tct gaa atc ggc 1267Arg Val Glu Arg Ala Gly Gly Lys Val Arg Ile Lys Ser Glu Ile Gly
375 380 385gaa ggc acc gaa gtg gca atc acc atg gat gtg tagttggtcg tacgcgcgtg 1320Glu Gly Thr Glu Val Ala Ile Thr Met Asp Val390 395 400tct1323<210>306<211>400<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>306Met Ile Glu Asp Met Ser Asn Thr Pro Ala Pro Tyr Thr Pro Gln Pro1 5 10 15Ala Gly Gln Ala Val Pro Leu Tyr Pro Thr Phe Thr Arg Ser Arg Asp
20 25 30Gly Arg Val Val Ala Gly Val Ala Ser Gly Leu Ala Lys His Leu Asn
35 40 45Val Ser Val Phe Trp Val Arg Ala Leu Leu Ile Phe Ala Ala Leu Leu
50 55 60Ser Gly Ala Gly Leu Phe Ala Tyr Ala Leu Ile Trp Ile Phe Thr Arg65 70 75 80Ile Glu Lys Lys Gly Ser Gly Glu Ala Ser Thr Ser Lys Arg Trp Val
85 90 95Ser Trp Cys Leu Val Leu Leu Ala Ile Gly Gly Ala Ala Ala Ser Val
100 105 110Met Leu Ser Thr Gly Phe Ala Val Gly Thr Leu Val Pro Ile Gly Val
115 120 125Val Gly Val Gly Leu Leu Met Val Trp Leu Ala Tyr Asp Arg Gly Val
130 135 140Glu Ser Gly Pro Asn Leu Leu Ile Ile Ala Thr Gly Gly Val Leu Met145 150 155 160Leu Val Ala Ile Val Leu Ile Val Met Asn Trp Asn Thr Gln Asp Gly
165 170 175Phe Val Met Ala Leu Val Ala Val Val Leu Thr Leu Val Gly Val Ala
180 185 190Ala Leu Gly Val Pro Leu Trp Val Arg Met Trp Asp Gln Leu Gly Glu
195 200 205Glu Arg Ala Glu Lys Ala Ala Ala Ala Glu Arg Ala Asp Ile Ala Ser
210 215 220Arg Leu His Asp Ser Val Leu Gln Thr Leu Ala Leu Ile Gln Lys Arg225 230 235 240Ala Asp Asp Pro Ala Glu Val Ala Arg Leu Ala Arg Gly Gln Glu Arg
245 250 255Glu Leu Arg Gln Trp Leu Phe Asp Ser Gln Asp Lys Thr Pro Gln Thr
260 265 270Thr Gly Thr Val Phe Thr Ala Leu Glu Arg Ala Cys Gly Glu Val Glu
275 280 285Asp Ile Tyr Ala Leu Arg Ile Val Pro Val Thr Val Gly Thr Asp Glu
290 295 300Ala Leu Thr Glu Lys Thr Gln Ala Ala Val Met Ala Val Arg Glu Ala305 310 315 320Leu Val Asn Val Ala Lys His Ala Gly Val Glu Thr Ala Asp Val Tyr
325 330 335Ala Glu Ile Met Leu Gly Glu Leu Asn Ile Phe Val Arg Asp Arg Gly
340 345 350Ala Gly Phe Asp Pro Asp Asn Ile Pro Asp Gly His His Gly Leu Ala
355 360 365Glu Ser Val Gln Gly Arg Val Glu Arg Ala Gly Gly Lys Val Arg Ile
370 375 380Lys Ser Glu Ile Gly Glu Gly Thr Glu Val Ala Ile Thr Met Asp Val385 390 395 400<210>307<211>1119<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (1096)<223>RXA00756<400>307attcgctaaa cctgcgcagg atgagactgc cctcgcagaa agcacattcg acgaagccac 60cgcgtaaaca gtacgtggtg gaagcttgag aggaagacaa gtg aat att gat gtc 115
Val Asn Ile Asp Val
1 5cag gct tta aaa gcc atc gag tct gaa aaa gga atc cca gtt cca gac 163Gln Ala Leu Lys Ala Ile Glu Ser Glu Lys Gly Ile Pro Val Pro Asp
10 15 20ttg ctg cgc acc atc gcc tct gca ctt ttg cat tcg tac atg gat aat 211Leu Leu Arg Thr Ile Ala Ser Ala Leu Leu His Ser Tyr Met Asp Asn
25 30 35cgc gaa act gtt gcg tct gcg aac ctg aaa cca cgc gtg gac atc gat 259Arg Glu Thr Val Ala Ser Ala Asn Leu Lys Pro Arg Val Asp Ile Asp
40 45 50tcc aca act ggc acg gtc aac gtc atc gtc tca gaa ttc gac gaa aac 307Ser Thr Thr Gly Thr Val Asn Val Ile Val Ser Glu Phe Asp Glu Asn
55 60 65gga gag ctc gct tcc gaa tac gac gac acc cca tcc aac ttc gga cga 355Gly Glu Leu Ala Ser Glu Tyr Asp Asp Thr Pro Ser Asn Phe Gly Arg70 75 80 85gtc agc gcc cgc gct gtt cgc gac gcg atc gtt aag tcc ctg cgc gaa 403Val Ser Ala Arg Ala Val Arg Asp Ala Ile Val Lys Ser Leu Arg Glu
90 95 100gca gaa gca agc cga gca ttc gat gcg tac gca gat tat gaa ggc acc 451Ala Glu Ala Ser Arg Ala Phe Asp Ala Tyr Ala Asp Tyr Glu Gly Thr
105 110 115gtt gtg tcc ggc atc gtt caa gca gat gcc cgc gca gct gaa cgc gga 499Val Val Ser Gly Ile Val Gln Ala Asp Ala Arg Ala Ala Glu Arg Gly
120 125 130atc atc atc gtg cag ctg ggt acc gaa gcg gac aac caa gac ggc gtt 547Ile Ile Ile Val Gln Leu Gly Thr Glu Ala Asp Asn Gln Asp Gly Val
135 140 145ttg ctc cca gcc gag cag atc cct ggc gaa aag ctc aag cac ggc gac 595Leu Leu Pro Ala Glu Gln Ile Pro Gly Glu Lys Leu Lys His Gly Asp150 155 160 165cgc gtc aag tgc ttc gtc gtt ggc gtg ggc aag ggc aac act gac atc 643Arg Val Lys Cys Phe Val Val Gly Val Gly Lys Gly Asn Thr Asp Ile
170 175 180cag atc aac ctg tct cgt act cac cct gag ctg gtg cgc cga ctg ttt 691Gln Ile Asn Leu Ser Arg Thr His Pro Glu Leu Val Arg Arg Leu Phe
185 190 195gaa ctg gaa atc cca gaa gtt gct gac gga tcc gtg gaa att gtt gct 739Glu Leu Glu Ile Pro Glu Val Ala Asp Gly Ser Val Glu Ile Val Ala
200 205 210atc tcc cgc gaa gcc gga cac cgc tcc aag gtt gct gtt caa gcc aag 787Ile Ser Arg Glu Ala Gly His Arg Ser Lys Val Ala Val Gln Ala Lys
215 220 225gtg aag aac ctc aac gcc aag ggc gct tgc att ggc cca cgt gga cag 835Val Lys Asn Leu Asn Ala Lys Gly Ala Cys Ile Gly Pro Arg Gly Gln230 235 240 245cgt gtg tcc aac atc atg cgt gaa ctc ggt gga gaa aaa atc gac atc 883Arg Val Ser Asn Ile Met Arg Glu Leu Gly Gly Glu Lys Ile Asp Ile
250 255 260atc gat tac tcc gaa gat cca gca acc ttc gtt gga aat gca ctg gca 931Ile Asp Tyr Ser Glu Asp Pro Ala Thr Phe Val Gly Asn Ala Leu Ala
265 270 275cca tcc aag gtt gtc aac gta gag gtc acc gat ctt gaa gct caa acc 979Pro Ser Lys Val Val Asn Val Glu Val Thr Asp Leu Glu Ala Gln Thr
280 285 290gcg cgc gta act gtc cct gac tac cag ctt tca cta gca atc ggt aaa 1027Ala Arg Val Thr Val Pro Asp Tyr Gln Leu Ser Leu Ala Ile Gly Lys
295 300 305gaa ggt caa aac gcc cgc ttg gct gcc cgc ctg acc ggc tgg aag atc 1075Glu Gly Gln Asn Ala Arg Leu Ala Ala Arg Leu Thr Gly Trp Lys Ile310 315 320 325gac atc cac tct gac atc gat taaaagtcgc ttgaaccggc atg 1119Asp Ile His Ser Asp Ile Asp
330<210>308<211>332<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>308Val Asn Ile Asp Val Gln Ala Leu Lys Ala Ile Glu Ser Glu Lys Gly1 5 10 15Ile Pro Val Pro Asp Leu Leu Arg Thr Ile Ala Ser Ala Leu Leu His
20 25 30Ser Tyr Met Asp Asn Arg Glu Thr Val Ala Ser Ala Asn Leu Lys Pro
35 40 45Arg Val Asp Ile Asp Ser Thr Thr Gly Thr Val Asn Val Ile Val Ser
50 55 60Glu Phe Asp Glu Asn Gly Glu Leu Ala Ser Glu Tyr Asp Asp Thr Pro65 70 75 80Ser Asn Phe Gly Arg Val Ser Ala Arg Ala Val Arg Asp Ala Ile Val
85 90 95Lys Ser Leu Arg Glu Ala Glu Ala Ser Arg Ala Phe Asp Ala Tyr Ala
100 105 110Asp Tyr Glu Gly Thr Val Val Ser Gly Ile Val Gln Ala Asp Ala Arg
115 120 125Ala Ala Glu Arg Gly Ile Ile Ile Val Gln Leu Gly Thr Glu Ala Asp
130 135 140Asn Gln Asp Gly Val Leu Leu Pro Ala Glu Gln Ile Pro Gly Glu Lys145 150 155 160Leu Lys His Gly Asp Arg Val Lys Cys Phe Val Val Gly Val Gly Lys
165 170 175Gly Asn Thr Asp Ile Gln Ile Asn Leu Ser Arg Thr His Pro Glu Leu
180 185 190Val Arg Arg Leu Phe Glu Leu Glu Ile Pro Glu Val Ala Asp Gly Ser
195 200 205Val Glu Ile Val Ala Ile Ser Arg Glu Ala Gly His Arg Ser Lys Val
210 215 220Ala Val Gln Ala Lys Val Lys Asn Leu Asn Ala Lys Gly Ala Cys Ile225 230 235 240Gly Pro Arg Gly Gln Arg Val Ser Asn Ile Met Arg Glu Leu Gly Gly
245 250 255Glu Lys Ile Asp Ile Ile Asp Tyr Ser Glu Asp Pro Ala Thr Phe Val
260 265 270Gly Asn Ala Leu Ala Pro Ser Lys Val Val Asn Val Glu Val Thr Asp
275 280 285Leu Glu Ala Gln Thr Ala Arg Val Thr Val Pro Asp Tyr Gln Leu Ser
290 295 300Leu Ala Ile Gly Lys Glu Gly Gln Asn Ala Arg Leu Ala Ala Arg Leu305 310 315 320Thr Gly Trp Lys Ile Asp Ile His Ser Asp Ile Asp
325 330<210>309<211>834<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101).. (811)<223>RXA00139<400>309gaccggaaac gtactcaagg tagacacccg cgacggttcc tacctctccc gcgttaacaa 60ctaagattct taaaaccttt aagaatcagc cagaaacatt ttg att gaa cag gaa 115
Leu Ile Glu Gln Glu
1 5caa aga gaa caa aac gtg agc gag cgt cga caa gat tac aag cga cac 163Gln Arg Glu Gln Asn Val Ser Glu Arg Arg Gln Asp Tyr Lys Arg His
10 15 20gga tcc cgc tac aag gcg cgc atg cgt gcc gta gac atc cta ttt gaa 211Gly Ser Arg Tyr Lys Ala Arg Met Arg Ala Val Asp Ile Leu Phe Glu
25 30 35gcg gaa tcc cgc gat gtt gat ccc gtg gcc atc atc gat gac cgc cac 259Ala Glu Ser Arg Asp Val Asp Pro Val Ala Ile Ile Asp Asp Arg His
40 45 50aag ttg gcg cgc gat acc aac ccc atc gtt gca ccg gta gcg gaa tac 307Lys Leu Ala Arg Asp Thr Asn Pro Ile Val Ala Pro Val Ala Glu Tyr
55 60 65acc gaa acc atc atc aat ggc gtt gcc gtt gaa ctc gat acc ctc gat 355Thr Glu Thr Ile Ile Asn Gly Val Ala Val Glu Leu Asp Thr Leu Asp70 75 80 85gtc ttc ctc gcg gaa cac atc gca gaa acc tgg act ctc gga cga ctc 403Val Phe Leu Ala Glu His Ile Ala Glu Thr Trp Thr Leu Gly Arg Leu
90 95 100cca tcc gtc gac cgc gca atc ctg cgc gtc gct tcc tgg gaa atg atc 451Pro Ser Val Asp Arg Ala Ile Leu Arg Val Ala Ser Trp Glu Met Ile
105 110 115tac aac gcc gac gtt cct gtc acc acc gca atc gtt gaa gcc gtg gaa 499Tyr Asn Ala Asp Val Pro Val Thr Thr Ala Ile Val Glu Ala Val Glu
120 125 130att gcc tcc gaa tac tcc gga gac aaa tcc agt gcc tac atc aac gcg 547Ile Ala Ser Glu Tyr Ser Gly Asp Lys Ser Ser Ala Tyr Ile Asn Ala
135 140 145aca ctt gac gcc atg gca tca aag gtg gag acc ctc cgc gag cgc gcc 595Thr Leu Asp Ala Met Ala Ser Lys Val Glu Thr Leu Arg Glu Arg Ala150 155 160 165gcc aac cca gaa gca gtt ctg gcg gaa gct tcc gaa tct ctc gat gat 643Ala Asn Pro Glu Ala Val Leu Ala Glu Ala Ser Glu Ser Leu Asp Asp
170 175 180gct ccg gtc gcg ccg tgg gat gac tcg gat gct ttg gat gac tcg gat 691Ala Pro Val Ala Pro Trp Asp Asp Ser Asp Ala Leu Asp Asp Ser Asp
185 190 195gaa gat ttt gag gct gta gat gct gct gag gtt ttt gag gct gaa gag 739Glu Asp Phe Glu Ala Val Asp Ala Ala Glu Val Phe Glu Ala Glu Glu
200 205 210act gta gag gtt tcc gaa gtc gca gaa gac tct gaa gtt tca aag gtt 787Thr Val Glu Val Ser Glu Val Ala Glu Asp Ser Glu Val Ser Lys Val
215 220 225tca gaa gaa aag gct gac gag agc taaatctttt ctggctaaca cca 834Ser Glu Glu Lys Ala Asp Glu Ser230 235<210>310<211>237<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>310Leu Ile Glu Gln Glu Gln Arg Glu Gln Asn Val Ser Glu Arg Arg Gln1 5 10 15Asp Tyr Lys Arg His Gly Ser Arg Tyr Lys Ala Arg Met Arg Ala Val
20 25 30Asp Ile Leu Phe Glu Ala Glu Ser Arg Asp Val Asp Pro Val Ala Ile
35 40 45Ile Asp Asp Arg His Lys Leu Ala Arg Asp Thr Asn Pro Ile Val Ala
50 55 60Pro Val Ala Glu Tyr Thr Glu Thr Ile Ile Asn Gly Val Ala Val Glu65 70 75 80Leu Asp Thr Leu Asp Val Phe Leu Ala Glu His Ile Ala Glu Thr Trp
85 90 95Thr Leu Gly Arg Leu Pro Ser Val Asp Arg Ala Ile Leu Arg Val Ala
100 105 110Ser Trp Glu Met Ile Tyr Asn Ala Asp Val Pro Val Thr Thr Ala Ile
115 120 125Val Glu Ala Val Glu Ile Ala Ser Glu Tyr Ser Gly Asp Lys Ser Ser
130 135 140Ala Tyr Ile Asn Ala Thr Leu Asp Ala Met Ala Ser Lys Val Glu Thr145 150 155 160Leu Arg Glu Arg Ala Ala Asn Pro Glu Ala Val Leu Ala Glu Ala Ser
165 170 175Glu Ser Leu Asp Asp Ala Pro Val Ala Pro Trp Asp Asp Ser Asp Ala
180 185 190Leu Asp Asp Ser Asp Glu Asp Phe Glu Ala Val Asp Ala Ala Glu Val
195 200 205Phe Glu Ala Glu Glu Thr Val Glu Val Ser Glu Val Ala Glu Asp Ser
210 215 220Glu Val Ser Lys Val Ser Glu Glu Lys Ala Asp Glu Ser225 230 235<210>311<211>1458<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1435)<223>RXA01303<400>311aacatgcggg cgcaggtcag agctgt tatc ttagtactta tcacagccat agggcgggct 60tgacggaaag cctttccgcg taaccatgaa gaggcatcac gtg aca caa ctc aac 115
Val Thr Gln Leu Asn
1 5acc aaa ggc gtt gtt ctg caa ggg tgg gat cca gaa gat cct gaa cat 163Thr Lys Gly Val Val Leu Gln Gly Trp Asp Pro Glu Asp Pro Glu His
10 15 20tgg gac tcg aaa att gca tgg cga acc ctg tgg att acc acc ttc tcc 211Trp Asp Ser Lys Ile Ala Trp Arg Thr Leu Trp Ile Thr Thr Phe Ser
25 30 35atg att att ggg ttc tgc gtg tgg tat ttg gtt tct gcc atc gct ccc 259Met Ile Ile Gly Phe Cys Val Trp Tyr Leu Val Ser Ala Ile Ala Pro
40 45 50cta ctc aat cga att gga ttt gat ctc tca gca ggt cag ctt tat tgg 307Leu Leu Asn Arg Ile Gly Phe Asp Leu Ser Ala Gly Gln Leu Tyr Trp
55 60 65ctc gca tct atc ccc ggt ttg gcc ggc gga tta atc cga ttg att tac 355Leu Ala Ser Ile Pro Gly Leu Ala Gly Gly Leu Ile Arg Leu Ile Tyr70 75 80 85atg ttc ctt cca ccg att ctt gga acc cgc aaa ttg gtc gga att tcc 403Met Phe Leu Pro Pro Ile Leu Gly Thr Arg Lys Leu Val Gly Ile Ser
90 95 100tcc ggt cta ttt ttg atc ccc atg ttt ggg tgg ttc ctg gct gtc caa 451Ser Gly Leu Phe Leu Ile Pro Met Phe Gly Trp Phe Leu Ala Val Gln
105 110 115gat tca agc act ccc tac tgg tgg ctt ctc aca ctc gct gca ctc act 499Asp Ser Ser Thr Pro Tyr Trp Trp Leu Leu Thr Leu Ala Ala Leu Thr
120 125 130ggc att ggt ggt ggc gtg ttc tct gga tat atg ccg tcc acg gga tac 547Gly Ile Gly Gly Gly Val Phe Ser Gly Tyr Met Pro Ser Thr Gly Tyr
135 140 145ttc ttc ccc aag gca aaa tcg ggc act gcg ctg ggc att cag gca ggt 595Phe Phe Pro Lys Ala Lys Ser Gly Thr Ala Leu Gly Ile Gln Ala Gly150 155 160 165atc ggc aac ctc ggc gtc tcg ata att cag ttc atg ggc cca tgg gtc 643Ile Gly Asn Leu Gly Val Ser Ile Ile Gln Phe Met Gly Pro Trp Val
170 175 180atg ggt ttc ggt ctg ctg ggc att ggt ttc ctc acc ccg cag cgc acc 691Met Gly Phe Gly Leu Leu Gly Ile Gly Phe Leu Thr Pro Gln Arg Thr
185 190 195att gaa ggc acc acg gtg ttt gtg cac aat gct gcg att gtg ttg gtc 739Ile Glu Gly Thr Thr Val Phe Val His Asn Ala Ala Ile Val Leu Val
200 205 210ccg tgg act att ctc gcg gcc gtt tta tcc ttc ctg ttt ctt aaa gat 787Pro Trp Thr Ile Leu Ala Ala Val Leu Ser Phe Leu Phe Leu Lys Asp
215 220 225gtc cca gtc acc gca aat ttc cgg caa cag atc gat atc ttt ggc aac 835Val Pro Val Thr Ala Asn Phe Arg Gln Gln Ile Asp Ile Phe Gly Asn230 235 240 245aag aac aca tgg att ttg tcc att atc tac ttg atg aca ttc ggt gcc 883Lys Asn Thr Trp Ile Leu Ser Ile Ile Tyr Leu Met Thr Phe Gly Ala
250 255 260ttc gcc ggt ttc gcc gcg cag ttc ggt ctg atc atc aac aac aac ttc 931Phe Ala Gly Phe Ala Ala Gln Phe Gly Leu Ile Ile Asn Asn Asn Phe
265 270 275ggc atc gct tcc ccg atg gca gag act tat cca gct gag atg ctt cac 979Gly Ile Ala Ser Pro Met Ala Glu Thr Tyr Pro Ala Glu Met Leu His
280 285 290gcc ggt gct acg ttc gcg ttt ctt gga cct ttg att ggt gct ttg gtg 1027Ala Gly Ala Thr Phe Ala Phe Leu Gly Pro Leu Ile Gly Ala Leu Val
295 300 305cgt gct gca tgg ggt cca ctg tgt gac aga ttc ggt gga gct atc tgg 1075Arg Ala Ala Trp Gly Pro Leu Cys Asp Arg Phe Gly Gly Ala Ile Trp310 315 320 325acc ttt gtc ggt ggc atc gga atg act atc gcc act gca gct gcc gca 1123Thr Phe Val Gly Gly Ile Gly Met Thr Ile Ala Thr Ala Ala Ala Ala
330 335 340atc ttc cta agc aga gcg gag aca cct gat gat ttc tgg cca ttc ctg 1171Ile Phe Leu Ser Arg Ala Glu Thr Pro Asp Asp Phe Trp Pro Phe Leu
345 350 355tgg tcc atg ctt gcc ctg ttc ttc ttc acc ggt ctg ggc aat gct ggc 1219Trp Ser Met Leu Ala Leu Phe Phe Phe Thr Gly Leu Gly Asn Ala Gly
360 365 370acc ttc aaa caa atg ccc atg att ttg cct aaa cgc caa gca ggt ggc 1267Thr Phe Lys Gln Met Pro Met Ile Leu Pro Lys Arg Gln Ala Gly Gly
375 380 385gtg atc ggc tgg acc ggt gcc att ggt gcc ttc ggc ccc ttc att gtc 1315Val Ile Gly Trp Thr Gly Ala Ile Gly Ala Phe Gly Pro Phe Ile Val390 395 400 405ggt gtc ttg ctc tcc ttc act cca act gtc gcg ttc ttc tgg ggc tgc 1363Gly Val Leu Leu Ser Phe Thr Pro Thr Val Ala Phe Phe Trp Gly Cys
410 415 420gtg gtg ttc ttc atc atc gcc acc gct ttg acc tgg atc tac tac gcc 1411Val Val Phe Phe Ile Ile Ala Thr Ala Leu Thr Trp Ile Tyr Tyr Ala
425 430 435cgc ccg aac gct cca ttc ccg gga taaaccgaaa ggccaatcca tga 1458Arg Pro Asn Ala Pro Phe Pro Gly
440 445<210>312<211>445<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>312Val Thr Gln Leu Asn Thr Lys Gly Val Val Leu Gln Gly Trp Asp Pro1 5 10 15Glu Asp Pro Glu His Trp Asp Ser Lys Ile Ala Trp Arg Thr Leu Trp
20 25 30Ile Thr Thr Phe Ser Met Ile Ile Gly Phe Cys Val Trp Tyr Leu Val
35 40 45Ser Ala Ile Ala Pro Leu Leu Asn Arg Ile Gly Phe Asp Leu Ser Ala
50 55 60Gly Gln Leu Tyr Trp Leu Ala Ser Ile Pro Gly Leu Ala Gly Gly Leu65 70 75 80Ile Arg Leu Ile Tyr Met Phe Leu Pro Pro Ile Leu Gly Thr Arg Lys
85 90 95Leu Val Gly Ile Ser Ser Gly Leu Phe Leu Ile Pro Met Phe Gly Trp
100 105 110Phe Leu Ala Val Gln Asp Ser Ser Thr Pro Tyr Trp Trp Leu Leu Thr
115 120 125Leu Ala Ala Leu Thr Gly Ile Gly Gly Gly Val Phe Ser Gly Tyr Met
130 135 140Pro Ser Thr Gly Tyr Phe Phe Pro Lys Ala Lys Ser Gly Thr Ala Leu145 150 155 160Gly Ile Gln Ala Gly Ile Gly Asn Leu Gly Val Ser Ile Ile Gln Phe
165 170 175Met Gly Pro Trp Val Met Gly Phe Gly Leu Leu Gly Ile Gly Phe Leu
180 185 190Thr Pro Gln Arg Thr Ile Glu Gly Thr Thr Val Phe Val His Asn Ala
195 200 205Ala Ile Val Leu Val Pro Trp Thr Ile Leu Ala Ala Val Leu Ser Phe
210 215 220Leu Phe Leu Lys Asp Val Pro Val Thr Ala Asn Phe Arg Gln Gln Ile225 230 235 240Asp Ile Phe Gly Asn Lys Asn Thr Trp Ile Leu Ser Ile Ile Tyr Leu
245 250 255Met Thr Phe Gly Ala Phe Ala Gly Phe Ala Ala Gln Phe Gly Leu Ile
260 265 270Ile Asn Asn Asn Phe Gly Ile Ala Ser Pro Met Ala Glu Thr Tyr Pro
275 280 285Ala Glu Met Leu His Ala Gly Ala Thr Phe Ala Phe Leu Gly Pro Leu
290 295 300Ile Gly Ala Leu Val Arg Ala Ala Trp Gly Pro Leu Cys Asp Arg Phe305 310 315 320Gly Gly Ala Ile Trp Thr Phe Val Gly Gly Ile Gly Met Thr Ile Ala
325 330 335Thr Ala Ala Ala Ala Ile Phe Leu Ser Arg Ala Glu Thr Pro Asp Asp
340 345 350Phe Trp Pro Phe Leu Trp Ser Met Leu Ala Leu Phe Phe Phe Thr Gly
355 360 365Leu Gly Asn Ala Gly Thr Phe Lys Gln Met Pro Met Ile Leu Pro Lys
370 375 380Arg Gln Ala Gly Gly Val Ile Gly Trp Thr Gly Ala Ile Gly Ala Phe385 390 395 400Gly Pro Phe Ile Val Gly Val Leu Leu Ser Phe Thr Pro Thr Val Ala
405 410 415Phe Phe Trp Gly Cys Val Val Phe Phe Ile Ile Ala Thr Ala Leu Thr
420 425 430Trp Ile Tyr Tyr Ala Arg Pro Asn Ala Pro Phe Pro Gly
435 440 445<210>313<211>327<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(304)<223>RXA01412<400>313cgccttcttt gcagctgaga attaactcac ccttgccctt ataccccata ggggtatagc 60cttgagggag agagtacttc acctgaaagg ggccagtgac atg gca tta aag aac 115
Met Ala Leu Lys Asn
1 5tac acc gtt gag ggc atg acc tgc gca cac tgc gtg gca tcg gta act 163Tyr Thr Val Glu Gly Met Thr Cys Ala His Cys Val Ala Ser Val Thr
10 15 20gaa gag gta agc gaa gtt aat ggc gtt agc gct gtt gac gtc act cta 211Glu Glu Val Ser Glu Val Asn Gly Val Ser Ala Val Asp Val Thr Leu
25 30 35gaa tca gga aac gtc gct gtc agt ggc gaa ggt ttc agc gat gca gag 259Glu Ser Gly Asn Val Ala Val Ser Gly Glu Gly Phe Ser Asp Ala Glu
40 45 50atc cag gct gct gta gag gaa gcc ggc tac aag atc gtt gcc tcc 304Ile Gln Ala Ala Val Glu Glu Ala Gly Tyr Lys Ile Val Ala Ser
55 60 65taaagcaccc aagaacattt aaa 327<210>314<211>68<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>314Met Ala Leu Lys Asn Tyr Thr Val Glu Gly Met Thr Cys Ala His Cysl 5 10 15Val Ala Ser Val Thr Glu Glu Val Ser Glu Val Asn Gly Val Ser Ala
20 25 30Val Asp Val Thr Leu Glu Ser Gly Asn Val Ala Val Ser Gly Glu Gly
35 40 45Phe Ser Asp Ala Glu Ile Gln Ala Ala Val Glu Glu Ala Gly Tyr Lys
50 55 60Ile Val Ala Ser65<210>3l5<211>1266<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1243)<223>RXA00773<400>3l5gcccccaaaa agtgaaagca caccactttc ctagttgcgc cctgctcaca atttgcttca 60aatattttgc ccaacctgat tcacggggga caatagttag gtg act tta aaa atc 115
Val Thr Leu Lys Ile
1 5ggc ccc ttt gac ctt gcc tcc cct gtg gtt cta gcc ccc atg gct ggt 163Gly Pro Phe Asp Leu Ala Ser Pro Val Val Leu Ala Pro Met Ala Gly
10 15 20gta acc aac gtt gct ttc cgc acg ctg tgc cgt gaa cag gaa atg caa 211Val Thr Asn Val Ala Phe Arg Thr Leu Cys Arg Glu Gln Glu Met Gln
25 30 35cgc acg gga aca atc tcg ggg ctg tac gtc tgt gaa atg gtg act gcg 259Arg Thr Gly Thr Ile Ser Gly Leu Tyr Val Cys Glu Met Val Thr Ala
40 45 50cgt gct ctt gtt gag cgc aat gag aaa acc atg cac atg acc acc ttc 307Arg Ala Leu Val Glu Arg Asn Glu Lys Thr Met His Met Thr Thr Phe
55 60 65gcg ccg gat gaa aat ccc cga agc ttg cag ctg tac acg gtt gac ccg 355Ala Pro Asp Glu Asn Pro Arg Ser Leu Gln Leu Tyr Thr Val Asp Pro70 75 80 85aag tac acc tac gaa gcg gcg aag atg atc gtt gat gaa aac ttg gcg 403Lys Tyr Thr Tyr Glu Ala Ala Lys Met Ile Val Asp Glu Asn Leu Ala
90 95 100gat cat att gat atg aac ttt ggc tgc ccg gtt cca aag gtc acg cgc 451Asp His Ile Asp Met Asn Phe Gly Cys Pro Val Pro Lys Val Thr Arg
105 110 115cgg ggt ggc ggt tct gcg att cct tac aag cgc cgt ttg ttt gaa aac 499Arg Gly Gly Gly Ser Ala Ile Pro Tyr Lys Arg Arg Leu Phe Glu Asn
120 125 130atc gtt tcc gcg gct gtg aag gct acg gaa ggc acg gac att ccg gtg 547Ile Val Ser Ala Ala Val Lys Ala Thr Glu Gly Thr Asp Ile Pro Val
135 140 145acg gtg aag ttc cgc gtt ggt att gat gat gag cac cat act cac ttg 595Thr Val Lys Phe Arg Val Gly Ile Asp Asp Glu His His Thr His Leu150 155 160 165gat gct gga cgc att gct gtc gac gcc ggc gcg aag tcc gta gcg ctt 643Asp Ala Gly Arg Ile Ala Val Asp Ala Gly Ala Lys Ser Val Ala Leu
170 175 180cac gcc cgc act gcg gcg cag cgc tat tcc ggt gag gct gat tgg aac 691His Ala Arg Thr Ala Ala Gln Arg Tyr Ser Gly Glu Ala Asp Trp Asn
185 190 195gag atc gcg cgc ctg aag gag cat ttg gca gat acc ggc atc cca gtt 739Glu Ile Ala Arg Leu Lys Glu His Leu Ala Asp Thr Gly Ile Pro Val
200 205 210ttg ggc aat ggc gat att ttc gcg gca tcc gat gca acg cgc atg atg 787Leu Gly Asn Gly Asp Ile Phe Ala Ala Ser Asp Ala Thr Arg Met Met
215 220 225gag caa act ggc tgc gat ggc gtc gtg gtt ggg cgt ggt tgc ctg ggc 835Glu Gln Thr Gly Cys Asp Gly Val Val Val Gly Arg Gly Cys Leu Gly230 235 240 245agg cct tgg ctc ttt gct gag ctg tct gct gct gtt cgt gga gaa gaa 883Arg Pro Trp Leu Phe Ala Glu Leu Ser Ala Ala Val Arg Gly Glu Glu
250 255 260atc cca gag gag cct acc ttc ggc gaa gtt acc caa atc atc ctg cgc 931Ile Pro Glu Glu Pro Thr Phe Gly Glu Val Thr Gln Ile Ile Leu Arg
265 270 275cac gca gaa ctc ctc atg cag cat gat ggc gaa acc aag ggg ctg cgc 979His Ala Glu Leu Leu Met Gln His Asp Gly Glu Thr Lys Gly Leu Arg
280 285 290gat ctg cgt aag cac atg ggt tgg tac ctg cgc ggt ttc cct gtt ggc 1027Asp Leu Arg Lys His Met Gly Trp Tyr Leu Arg Gly Phe Pro Val Gly
295 300 305ggc gaa ttc cgc tcc aat ctg gcc aag gtt tcc acc tat gtg gag ctt 1075Gly Glu Phe Arg Ser Asn Leu Ala Lys Val Ser Thr Tyr Val Glu Leu310 315 320 325gag gat ctc cta gca cca tgg gct gac tcc acc gcc aag gca gag gac 1123Glu Asp Leu Leu Ala Pro Trp Ala Asp Ser Thr Ala Lys Ala Glu Asp
330 335 340gcg gaa ggt gca cga ggt cga cag ggc gct cct gca aag gtg gca ctt 1171Ala Glu Gly Ala Arg Gly Arg Gln Gly Ala Pro Ala Lys Val Ala Leu
345 350 355cca gat ggc tgg ttg gac gat cct gag gat gcc act gtt cct aaa ggc 1219Pro Asp Gly Trp Leu Asp Asp Pro Glu Asp Ala Thr Val Pro Lys Gly
360 365 370gca gaa atg gaa aac tcc gga ggg tagttaattt aatacttacc ccc 1266Ala Glu Met Glu Asn Ser Gly Gly
375 380<210>316<211>381<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>316Val Thr Leu Lys Ile Gly Pro Phe Asp Leu Ala Ser Pro Val Val Leu1 5 10 15Ala Pro Met Ala Gly Val Thr Asn Val Ala Phe Arg Thr Leu Cys Arg
20 25 30Glu Gln Glu Met Gln Arg Thr Gly Thr Ile Ser Gly Leu Tyr Val Cys
35 40 45Glu Met Val Thr Ala Arg Ala Leu Val Glu Arg Asn Glu Lys Thr Met
50 55 60His Met Thr Thr Phe Ala Pro Asp Glu Asn Pro Arg Ser Leu Gln Leu65 70 75 80Tyr Thr Val Asp Pro Lys Tyr Thr Tyr Glu Ala Ala Lys Met Ile Val
85 90 95Asp Glu Asn Leu Ala Asp His Ile Asp Met Asn Phe Gly Cys Pro Val
100 105 110Pro Lys Val Thr Arg Arg Gly Gly Gly Ser Ala Ile Pro Tyr Lys Arg
115 120 125Arg Leu Phe Glu Asn Ile Val Ser Ala Ala Val Lys Ala Thr Glu Gly
130 135 140Thr Asp Ile Pro Val Thr Val Lys Phe Arg Val Gly Ile Asp Asp Glu145 150 155 160His His Thr His Leu Asp Ala Gly Arg Ile Ala Val Asp Ala Gly Ala
165 170 175Lys Ser Val Ala Leu His Ala Arg Thr Ala Ala Gln Arg Tyr Ser Gly
180 185 190Glu Ala Asp Trp Asn Glu Ile Ala Arg Leu Lys Glu His Leu Ala Asp
195 200 205Thr Gly Ile Pro Val Leu Gly Asn Gly Asp Ile Phe Ala Ala Ser Asp
210 215 220Ala Thr Arg Met Met Glu Gln Thr Gly Cys Asp Gly Val Val Val Gly225 230 235 240Arg Gly Cys Leu Gly Arg Pro Trp Leu Phe Ala Glu Leu Ser Ala Ala
245 250 255Val Arg Gly Glu Glu Ile Pro Glu Glu Pro Thr Phe Gly Glu Val Thr
260 265 270Gln Ile Ile Leu Arg His Ala Glu Leu Leu Met Gln His Asp Gly Glu
275 280 285Thr Lys Gly Leu Arg Asp Leu Arg Lys His Met Gly Trp Tyr Leu Arg
290 295 300Gly Phe Pro Val Gly Gly Glu Phe Arg Ser Asn Leu Ala Lys Val Ser305 310 315 320Thr Tyr Val Glu Leu Glu Asp Leu Leu Ala Pro Trp Ala Asp Ser Thr
325 330 335Ala Lys Ala Glu Asp Ala Glu Gly Ala Arg Gly Arg Gln Gly Ala Pro
340 345 350Ala Lys Val Ala Leu Pro Asp Gly Trp Leu Asp Asp Pro Glu Asp Ala
355 360 365Thr Val Pro Lys Gly Ala Glu Met Glu Ash Ser Gly Gly
370 375 380<210>317<211>290<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(267)<223>RXA02746<400>317ggt gtg cag ggc atg act gtc acc gaa acc caa ggc ttt ggc cag cag 48Gly Val Gln Gly Met Thr Val Thr Glu Thr Gln Gly Phe Gly Gln Gln1 5 10 15aaa ggc cac acc gag gtg tac cgt ggt gct gaa tac gct gtc gat ttt 96Lys Gly His Thr Glu Val Tyr Arg Gly Ala Glu Tyr Ala Val Asp Phe
20 25 30gtg cct aag gtc aag att gaa gtt att atc tcc gat gct cag gct gag 144Val Pro Lys Val Lys Ile Glu Val Ile Ile Ser Asp Ala Gln Ala Glu
35 40 45gaa gtc atc aac att atc gtc gag acc gca cgc acc ggc aaa gtc ggc 192Glu Val Ile Asn Ile Ile Val Glu Thr Ala Arg Thr Gly Lys Val Gly
50 55 60gac ggc aaa gtg tgg atg act aac atc gaa gag ctg gtt cgt gtt cgt 240Asp Gly Lys Val Trp Met Thr Asn Ile Glu Glu Leu Val Arg Val Arg65 70 75 80acc ggt gag cgc ggc gaa gca gcc ctt taaaaactta tgaataatcc 287Thr Gly Glu Arg Gly Glu Ala Ala Leu
85agc 290<210>318<211>89<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>318Gly Val Gln Gly Met Thr Val Thr Glu Thr Gln Gly Phe Gly Gln Gln1 5 10 15Lys Gly His Thr Glu Val Tyr Arg Gly Ala Glu Tyr Ala Val Asp Phe
20 25 30Val Pro Lys Val Lys Ile Glu Val Ile Ile Ser Asp Ala Gln Ala Glu
35 40 45Glu Val Ile Asn Ile Ile Val Glu Thr Ala Arg Thr Gly Lys Val Gly
50 55 60Asp Gly Lys Val Trp Met Thr Asn Ile Glu Glu Leu Val Arg Val Arg65 70 75 80Thr Gly Glu Arg Gly Glu Ala Ala Leu
85<210>319<211>902<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(879)<223>RXA02745<400>319gcc gga atc tcc ttc gaa gtc ccc cgc ggt caa gtt ttg gcc ctc ctg 48Ala Gly Ile Ser Phe Glu Val Pro Arg Gly Gln Val Leu Ala Leu Leu1 5 10 15gga cct aat ggc gca ggc aaa acc acc acc att gaa atg tgc gaa ggt 96Gly Pro Asn Gly Ala Gly Lys Thr Thr Thr Ile Glu Met Cys Glu Gly
20 25 30ttt acc gcc ccc acc tct ggc agc atc cga gtc ttg ggc atc gat cca 144Phe Thr Ala Pro Thr Ser Gly Ser Ile Arg Val Leu Gly Ile Asp Pro
35 40 45gcc aca gaa cca gac cag gtg cgc cga cgc atc ggc atc atg ctt caa 192Ala Thr Glu Pro Asp Gln Val Arg Arg Arg Ile Gly Ile Met Leu Gln
50 55 60ggt ggc ggt tcc tac agc gga atc cgc gtg ttt gaa atg ctc aag ctt 240Gly Gly Gly Ser Tyr Ser Gly Ile Arg Val Phe Glu Met Leu Lys Leu65 70 75 80gcg gcg tcc tac aac gac aac cca cac gat cct gaa tgg ctg ctt gat 288Ala Ala Ser Tyr Asn Asp Asn Pro His Asp Pro Glu Trp Leu Leu Asp
85 90 95ctt gta gga ctg cgt gaa caa cgc aaa acc acc tac cga cgt ctg tca 336Leu Val Gly Leu Arg Glu Gln Arg Lys Thr Thr Tyr Arg Arg Leu Ser
100 105 110ggt ggc caa cag caa cgc ctt tct ttg gcc tta gca tta att ggt cgc 384Gly Gly Gln Gln Gln Arg Leu Ser Leu Ala Leu Ala Leu Ile Gly Arg
115 120 125cct gag att atc ttc ctc gac gaa ccc acc gct ggc atg gat gcg caa 432Pro Glu Ile Ile Phe Leu Asp Glu Pro Thr Ala Gly Met Asp Ala Gln
130 135 140tca cgc aac atg gtg tgg gag ctt gtc aac gat ctc cgc cgc gac ggc 480Ser Arg Asn Met Val Trp Glu Leu Val Asn Asp Leu Arg Arg Asp Gly145 150 155 160gtc acc atc gtg ctc acc acc cac ctg atg gat gag gcc gaa gca cta 528Val Thr Ile Val Leu Thr Thr His Leu Met Asp Glu Ala Glu Ala Leu
165 170 175gct gac cac gtg atc atc gtt gcc aac ggt caa atc ctt gcc agt ggc 576Ala Asp His Val Ile Ile Val Ala Asn Gly Gln Ile Leu Ala Ser Gly
180 185 190aca cct gat gaa ctc act gcg caa cgc gat cat ctt gaa att aat gtc 624Thr Pro Asp Glu Leu Thr Ala Gln Arg Asp His Leu Glu Ile Asn Val
195 200 205tcc gta gag acc acg agc ccg ctt gat ctt gat cgc ttg gtg gat gat 672Ser Val Glu Thr Thr Ser Pro Leu Asp Leu Asp Arg Leu Val Asp Asp
210 215 220ctc agc agc tta aac atc ggt gat gtg aaa gca cga gcc aac cgg cca 720Leu Ser Ser Leu Asn Ile Gly Asp Val Lys Ala Arg Ala Asn Arg Pro225 230 235 240ctg cat tat tca ctt cgg acg caa caa gcc acc ccg gat tcc ttg gcg 768Leu His Tyr Ser Leu Arg Thr Gln Gln Ala Thr Pro Asp Ser Leu Ala
245 250 255cac atc gtc cag gct gtc gcc cgc caa aac gtc atg att cgc tct ttg 816His Ile Val Gln Ala Val Ala Arg Gln Asn Val Met Ile Arg Ser Leu
260 265 270gat acg gga cac cgc tca ttg gaa gat gtc ttc ctg gac atc acc gga 864Asp Thr Gly His Arg Ser Leu Glu Asp Val Phe Leu Asp Ile Thr Gly
275 280 285aaa gaa ctg agg agt taacgcacac catgtctaaa cct 902Lys Glu Leu Arg Ser
290<210>320<211>293<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>320Ala Gly Ile Ser Phe Glu Val Pro Arg Gly Gln Val Leu Ala Leu Leu1 5 10 15Gly Pro Asn Gly Ala Gly Lys Thr Thr Thr Ile Glu Met Cys Glu Gly
20 25 30Phe Thr Ala Pro Thr Ser Gly Ser Ile Arg Val Leu Gly Ile Asp Pro
35 40 45Ala Thr Glu Pro Asp Gln Val Arg Arg Arg Ile Gly Ile Met Leu Gln
50 55 60Gly Gly Gly Ser Tyr Ser Gly Ile Arg Val Phe Glu Met Leu Lys Leu65 70 75 80Ala Ala Ser Tyr Asn Asp Asn Pro His Asp Pro Glu Trp Leu Leu Asp
85 90 95Leu Val Gly Leu Arg Glu Gln Arg Lys Thr Thr Tyr Arg Arg Leu Ser
100 105 110Gly Gly Gln Gln Gln Arg Leu Ser Leu Ala Leu Ala Leu Ile Gly Arg
115 120 125Pro Glu Ile Ile Phe Leu Asp Glu Pro Thr Ala Gly Met Asp Ala Gln
130 135 140Ser Arg Asn Met Val Trp Glu Leu Val Asn Asp Leu Arg Arg Asp Gly145 150 155 160Val Thr Ile Val Leu Thr Thr His Leu Met Asp Glu Ala Glu Ala Leu
165 170 175Ala Asp His Val Ile Ile Val Ala Asn Gly Gln Ile Leu Ala Ser Gly
180 185 190Thr Pro Asp Glu Leu Thr Ala Gln Arg Asp His Leu Glu Ile Asn Val
195 200 205Ser Val Glu Thr Thr Ser Pro Leu Asp Leu Asp Arg Leu Val Asp Asp
210 215 220Leu Ser Ser Leu Asn Ile Gly Asp Val Lys Ala Arg Ala Asn Arg Pro225 230 235 240Leu His Tyr Ser Leu Arg Thr Gln Gln Ala Thr Pro Asp Ser Leu Ala
245 250 255His Ile Val Gln Ala Val Ala Arg Gln Asn Val Met Ile Arg Ser Leu
260 265 270Asp Thr Gly His Arg Ser Leu Glu Asp Val Phe Leu Asp Ile Thr Gly
275 280 285Lys Glu Leu Arg Ser
290<210>321<211>486<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(463)<223>RXN00820<400>321acttccacca ccccaaacca tcgtttcttt cgaagacgca ccaaccctca ccggccagga 60cctgggcttt tcgcagtggc gcactgtcac ccaggagatg gtg aac acc ttg gcg 115
Val Asn Thr Leu Ala
1 5gac gca act gat gat cag cag tgg att cac act gat cct gag cgc gcc 163Asp Ala Thr Asp Asp Gln Gln Trp Ile His Thr Asp Pro Glu Arg Ala
10 15 20aag gac ggt cct ttt ggt ggc gca att gcc cac ggt ttc ctc acc ttg 211Lys Asp Gly Pro Phe Gly Gly Ala Ile Ala His Gly Phe Leu Thr Leu
25 30 35tcc atg atc att ccg ttc tgg ggc gag ctt ctc gat gtc acc ggc gtg 259Ser Met Ile Ile Pro Phe Trp Gly Glu Leu Leu Asp Val Thr Gly Val
40 45 50acc acc aag gtg aac tat ggc ctg gat aag gtg cgt ttc acc tct ccc 307Thr Thr Lys Val Asn Tyr Gly Leu Asp Lys Val Arg Phe Thr Ser Pro
55 60 65gtc aag gtc ggt tcc cgc atc cgc atg ggc gct gtg gtc cgt gag atc 355Val Lys Val Gly Ser Arg Ile Arg Met Gly Ala Val Val Arg Glu Ile70 75 80 85tct gag gtg aag ggc aat ggc ctg cac ctg gtc gcc gat ggc act att 403Ser Glu Val Lys Gly Asn Gly Leu His Leu Val Ala Asp Gly Thr Ile
90 95 100gag atc gaa ggg cag gag cgc ccg gcc gtc gta gct acc ttc ctc acc 451Glu Ile Glu Gly Gln Glu Arg Pro Ala Val Val Ala Thr Phe Leu Thr
105 110 115cgc ttc tac gct taaaagcttg cttctcgacg caa 486Arg Phe Tyr Ala
120<210>322<211>121<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>322Val Asn Thr Leu Ala Asp Ala Thr Asp Asp Gln Gln Trp Ile His Thr1 5 10 15Asp Pro Glu Arg Ala Lys Asp Gly Pro Phe Gly Gly Ala Ile Ala His
20 25 30Gly Phe Leu Thr Leu Ser Met Ile Ile Pro Phe Trp Gly Glu Leu Leu
35 40 45Asp Val Thr Gly Val Thr Thr Lys Val Asn Tyr Gly Leu Asp Lys Val
50 55 60Arg Phe Thr Ser Pro Val Lys Val Gly Ser Arg Ile Arg Met Gly Ala65 70 75 80Val Val Arg Glu Ile Ser Glu Val Lys Gly Asn Gly Leu His Leu Val
85 90 95Ala Asp Gly Thr Ile Glu Ile Glu Gly Gln Glu Arg Pro Ala Val Val
100 105 110Ala Thr Phe Leu Thr Arg Phe Tyr Ala
115 120<210>323<211>486<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(463)<223>FRXA00820<400>323acttccacca ccccaaacca tcgtttcttt cgaagacgca ccaaccctca ccggccagga 60cctgggcttt tcgcagtggc gcactgtcac ccaggagatg gtg aac acc ttc gcg 115
Val Asn Thr Phe Ala
1 5gac gca act gat gat cag cag tgg att cac act gat cct gag cgc gcc 163Asp Ala Thr Asp Asp Gln Gln Trp Ile His Thr Asp Pro Glu Arg Ala
10 15 20aag gac ggt cct ttt ggt ggc gca att gcc cac ggt ttc ctc acc ttg 211Lys Asp Gly Pro Phe Gly Gly Ala Ile Ala His Gly Phe Leu Thr Leu
25 30 35tcc atg atc att ccg ttc tgg ggc gag ctt ctc gat gtc acc ggc gtg 259Ser Met Ile Ile Pro Phe Trp Gly Glu Leu Leu Asp Val Thr Gly Val
40 45 50acc acc aag gtg aac tat ggc ctg gat aag gtg cgt ttc acc tct ccc 307Thr Thr Lys Val Asn Tyr Gly Leu Asp Lys Val Arg Phe Thr Ser Pro
55 60 65gtc aag gtc ggt tcc cgc atc cgc atg ggc gct gtg gtc cgt gag atc 355Val Lys Val Gly Ser Arg Ile Arg Met Gly Ala Val Val Arg Glu Ile70 75 80 85tct gag gtg aag ggc aat ggc ctg cac ctg gtc gcc gat ggc act att 403Ser Glu Val Lys Gly Asn Gly Leu His Leu Val Ala Asp Gly Thr Ile
90 95 100gag atc gaa ggg cag gag cgc ccg gcc gtc gta gct acc ttc ctc acc 451Glu Ile Glu Gly Gln Glu Arg Pro Ala Val Val Ala Thr Phe Leu Thr
105 110 115cgc ttc tac gct taaaagcttg cttctcgacg caa 486Arg Phe Tyr Ala
120<210>324<211>121<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>324Val Asn Thr Phe Ala Asp Ala Thr Asp Asp Gln Gln Trp Ile His Thr1 5 10 15Asp Pro Glu Arg Ala Lys Asp Gly Pro Phe Gly Gly Ala Ile Ala His
20 25 30Gly Phe Leu Thr Leu Ser Met Ile Ile Pro Phe Trp Gly Glu Leu Leu
35 40 45Asp Val Thr Gly Val Thr Thr Lys Val Asn Tyr Gly Leu Asp Lys Val
50 55 60Arg Phe Thr Ser Pro Val Lys Val Gly Ser Arg Ile Arg Met Gly Ala65 70 75 80Val Val Arg Glu Ile Ser Glu Val Lys Gly Asn Gly Leu His Leu Val
85 90 95Ala Asp Gly Thr Ile Glu Ile Glu Gly Gln Glu Arg Pro Ala Val Val
100 105 110Ala Thr Phe Leu Thr Arg Phe Tyr Ala
115 120<210>325<211>732<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(709)<223>RXA01059<400>325aattattagc tttccaatac aaaatttaaa tcccagagcg atctgcccca cacttacttg 60atgcgggaac aaatttgaag gtttttcagt tgctataggt atg act aca gtt act 115
Met Thr Thr Val Thr
1 5caa gac ctt cta gca ctt gac gaa gac gca cag aac ctc ctt ttc cgt 163Gln Asp Leu Leu Ala Leu Asp Glu Asp Ala Gln Asn Leu Leu Phe Arg
10 15 20gag gct cgc acc gca aat gct ttc act gat gaa cca atc tct gac gag 211Glu Ala Arg Thr Ala Asn Ala Phe Thr Asp Glu Pro Ile Ser Asp Glu
25 30 35cag atc gaa gca atc ttc gac cta gtt aag tgg gca cca acc gca atg 259Gln Ile Glu Ala Ile Phe Asp Leu Val Lys Trp Ala Pro Thr Ala Met
40 45 50aac tcc cag cct ctg cgc gtg gta att gtt cgt tcc gaa gaa gcc aaa 307Asn Ser Gln Pro Leu Arg Val Val Ile Val Arg Ser Glu Glu Ala Lys
55 60 65gct cgc ctc gtg cca ttg atg gca gaa ggc aac cag gcc aag gtt gct 355Ala Arg Leu Val Pro Leu Met Ala Glu Gly Asn Gln Ala Lys Val Ala70 75 80 85gca gct cct gcg gtc gca ctt ctt gca gcc gac atc gac ttc cac gaa 403Ala Ala Pro Ala Val Ala Leu Leu Ala Ala Asp Ile Asp Phe His Glu
90 95 100gaa atg ccc aag ctc ttc cca cct ttc cca ggc gca cgc gac atg ttc 451Glu Met Pro Lys Leu Phe Pro Pro Phe Pro Gly Ala Arg Asp Met Phe
105 110 115gaa gcc gat gaa gct tca cgt gct tcc tcc gca gaa ctc aat gct ggc 499Glu Ala Asp Glu Ala Ser Arg Ala Ser Ser Ala Glu Leu Asn Ala Gly
120 125 130ctt cag atc gga tac gcc atc atc ggt atc cgc gca gca ggt ctc gcc 547Leu Gln Ile Gly Tyr Ala Ile Ile Gly Ile Arg Ala Ala Gly Leu Ala
135 140 145gct ggc cca atg acc ggc atg gat gca gac gct atc tcc aag gag ttc 595Ala Gly Pro Met Thr Gly Met Asp Ala Asp Ala Ile Ser Lys Glu Phe150 155 160 165ttc cca gac ggc cgc cac cgc gtt ctg gtt gcc atc aac atg ggt aag 643Phe Pro Asp Gly Arg His Arg Val Leu Val Ala Ile Asn Met Gly Lys
170 175 180cca gct gac aat gct tgg tac gac cgc ctg cca cgc ctt gag cag gac 691Pro Ala Asp Asn Ala Trp Tyr Asp Arg Leu Pro Arg Leu Glu Gln Asp
185 190 195gaa gtt gtc gaa acc ctc tagaaaccac tctagaaata gct 732Glu Val Val Glu Thr Leu
200<210>326<211>203<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>326Met Thr Thr Val Thr Gln Asp Leu Leu Ala Leu Asp Glu Asp Ala Gln1 5 10 15Asn Leu Leu Phe Arg Glu Ala Arg Thr Ala Asn Ala Phe Thr Asp Glu
20 25 30Pro Ile Ser Asp Glu Gln Ile Glu Ala Ile Phe Asp Leu Val Lys Trp
35 40 45Ala Pro Thr Ala Met Asn Ser Gln Pro Leu Arg Val Val Ile Val Arg
50 55 60Ser Glu Glu Ala Lys Ala Arg Leu Val Pro Leu Met Ala Glu Gly Asn65 70 75 80Gln Ala Lys Val Ala Ala Ala Pro Ala Val Ala Leu Leu Ala Ala Asp
85 90 95Ile Asp Phe His Glu Glu Met Pro Lys Leu Phe Pro Pro Phe Pro Gly
100 105 110Ala Arg Asp Met Phe Glu Ala Asp Glu Ala Ser Arg Ala Ser Ser Ala
115 120 125Glu Leu Asn Ala Gly Leu Gln Ile Gly Tyr Ala Ile Ile Gly Ile Arg
130 135 140Ala Ala Gly Leu Ala Ala Gly Pro Met Thr Gly Met Asp Ala Asp Ala145 150 155 160Ile Ser Lys Glu Phe Phe Pro Asp Gly Arg His Arg Val Leu Val Ala
165 170 175Ile Asn Met Gly Lys Pro Ala Asp Asn Ala Trp Tyr Asp Arg Leu Pro
180 185 190Arg Leu Glu Gln Asp Glu Val Val Glu Thr Leu
195 200<210>327<211>1053<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1030)<223>RXN01386<400>327ctctattgtg gtacgcacca tgactgctca accagcccac gagccgccaa ggcatctccg 60gttcagtacg agcgggatcc tcccggacaa tcgcgtccaa atg tgg gag ggc cac 115
Met Trp Glu Gly His
1 5aat gct cgc gca ttg ctt ccg ctc gac att aga acc att gac gat cgc 163Asn Ala Arg Ala Leu Leu Pro Leu Asp Ile Arg Thr Ile Asp Asp Arg
10 15 20ccc atg cag gcc tcc gaa acc aac ctg cac ctc cca tca atg cgg atg 211Pro Met Gln Ala Ser Glu Thr Asn Leu His Leu Pro Ser Met Arg Met
25 30 35gcg agc gta ttc ggg act tcg caa ttt gtc gag cgt tca gag agt ttc 259Ala Ser Val Phe Gly Thr Ser Gln Phe Val Glu Arg Ser Glu Ser Phe
40 45 50atc tca gaa aac ccc acg ggt gtg gtt gcg atc ttc ttt gcg act gaa 307Ile Ser Glu Asn Pro Thr Gly Val Val Ala Ile Phe Phe Ala Thr Glu
55 60 65ggt gaa gca gtc ttc ttc cac cgt ggt gga cat gta gcg ctt cgg cca 355Gly Glu Ala Val Phe Phe His Arg Gly Gly His Val Ala Leu Arg Pro70 75 80 85ggt cag gcc att gtt tac gac gcc gat agg cca ttc ctc cgc gga ttc 403Gly Gln Ala Ile Val Tyr Asp Ala Asp Arg Pro Phe Leu Arg Gly Phe
90 95 100aac aat cgc ttc cgc gag cta gtt ctc acc atc ccg aag cag cgc tac 451Asn Asn Arg Phe Arg Glu Leu Val Leu Thr Ile Pro Lys Gln Arg Tyr
105 110 115ctt gaa att gtt ggc tca aaa ggc cct gag ctt ccc gct att ttt gag 499Leu Glu Ile Val Gly Ser Lys Gly Pro Glu Leu Pro Ala Ile Phe Glu
120 125 130ttc gga gca aca gga acc gcc aat gaa caa gct tta gcg cga cta gtt 547Phe Gly Ala Thr Gly Thr Ala Asn Glu Gln Ala Leu Ala Arg Leu Val
135 140 145cag gaa tct cta cac agg att gaa agt ggc gag ccg aag cat atc gat 595Gln Glu Ser Leu His Arg Ile Glu Ser Gly Glu Pro Lys His Ile Asp150 155 160 165tcc agt gga cct tta gga aaa ccg tgg agc gat atc gag cac gag gcc 643Ser Ser Gly Pro Leu Gly Lys Pro Trp Ser Asp Ile Glu His Glu Ala
170 175 180cac gga ctt atc cgc aat gta ctt ggc gac gcc aca agt agc gaa gaa 691His Gly Leu Ile Arg Asn Val Leu Gly Asp Ala Thr Ser Ser Glu Glu
185 190 195ggc tta att tct gca gcc cag aga ttt att gac atc aat att tcc gaa 739Gly Leu Ile Ser Ala Ala Gln Arg Phe Ile Asp Ile Asn Ile Ser Glu
200 205 210agt gac tta caa gcg tcg cgg att gct gca gcc gtg gga atc agc gaa 787Ser Asp Leu Gln Ala Ser Arg Ile Ala Ala Ala Val Gly Ile Ser Glu
215 220 225cgc caa cta agt cga atc ttc tca gac tca gga caa act atc gga cgc 835Arg Gln Leu Ser Arg Ile Phe Ser Asp Ser Gly Gln Thr Ile Gly Arg230 235 240 245tac gtc cta aac acc cga ctg gat ttt gca aag gaa gcg ctg tcg aca 883Tyr Val Leu Asn Thr Arg Leu Asp Phe Ala Lys Glu Ala Leu Ser Thr
250 255 260ccg gag cga gac aag gtt tcg gtc agt gag atc ggt aag cgc ttt ggg 931Pro Glu Arg Asp Lys Val Ser Val Ser Glu Ile Gly Lys Arg Phe Gly
265 270 275ttc gct tcc cca agt cat ttc agt cgc acc ttc cgc gag cgg ttt gaa 979Phe Ala Ser Pro Ser His Phe Ser Arg Thr Phe Arg Glu Arg Phe Glu
280 285 290atg acg ccg ctt caa tgg agg aag gaa tcg cag cgt caa tcc ttt caa 1027Met Thr Pro Leu Gln Trp Arg Lys Glu Ser Gln Arg Gln Ser Phe Gln
295 300 305gag tgaggttttt gttctcaggc gga 1053Glu310<210>328<211>310<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>328Met Trp Glu Gly His Asn Ala Arg Ala Leu Leu Pro Leu Asp Ile Arg1 5 10 15Thr Ile Asp Asp Arg Pro Met Gln Ala Ser Glu Thr Asn Leu His Leu
20 25 30Pro Ser Met Arg Met Ala Ser Val Phe Gly Thr Ser Gln Phe Val Glu
35 40 45Arg Ser Glu Ser Phe Ile Ser Glu Asn Pro Thr Gly Val Val Ala Ile
50 55 60Phe Phe Ala Thr Glu Gly Glu Ala Val Phe Phe His Arg Gly Gly His65 70 75 80Val Ala Leu Arg Pro Gly Gln Ala Ile Val Tyr Asp Ala Asp Arg Pro
85 90 95Phe Leu Arg Gly Phe Asn Asn Arg Phe Arg Glu Leu Val Leu Thr Ile
100 105 110Pro Lys Gln Arg Tyr Leu Glu Ile Val Gly Ser Lys Gly Pro Glu Leu
115 120 125Pro Ala Ile Phe Glu Phe Gly Ala Thr Gly Thr Ala Asn Glu Gln Ala
130 135 140Leu Ala Arg Leu Val Gln Glu Ser Leu His Arg Ile Glu Ser Gly Glu145 150 155 160Pro Lys His Ile Asp Ser Ser Gly Pro Leu Gly Lys Pro Trp Ser Asp
165 170 175Ile Glu His Glu Ala His Gly Leu Ile Arg Asn Val Leu Gly Asp Ala
180 185 190Thr Ser Ser Glu Glu Gly Leu Ile Ser Ala Ala Gln Arg Phe Ile Asp
195 200 205Ile Asn Ile Ser Glu Ser Asp Leu Gln Ala Ser Arg Ile Ala Ala Ala
210 215 220Val Gly Ile Ser Glu Arg Gln Leu Ser Arg Ile Phe Ser Asp Ser Gly225 230 235 240Gln Thr Ile Gly Arg Tyr Val Leu Asn Thr Arg Leu Asp Phe Ala Lys
245 250 255Glu Ala Leu Ser Thr Pro Glu Arg Asp Lys Val Ser Val Ser Glu Ile
260 265 270Gly Lys Arg Phe Gly Phe Ala Ser Pro Ser His Phe Ser Arg Thr Phe
275 280 285Arg Glu Arg Phe Glu Met Thr Pro Leu Gln Trp Arg Lys Glu Ser Gln
290 295 300Arg Gln Ser Phe Gln Glu305 310<210>329<211>1806<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1783)<223>RXN00073<400>329gaatctaatg gttggtctag acagagcggt acgtctaagt ttgcggatag atcaaaccga 60gtgacatgta cttcactagc tctttaagga ttaactcccc atg aca aca acc acc 115
Met Thr Thr Thr Thr
1 5gga agt gcc cgg cca gca cgt gcc gcc agg aag cct aag ccc gaa ggc 163Gly Ser Ala Arg Pro Ala Arg Ala Ala Arg Lys Pro Lys Pro Glu Gly
10 15 20caa tgg aaa atc gac ggc acc gag ccg ctt aac cat gcc gag gaa att 211Gln Trp Lys Ile Asp Gly Thr Glu Pro Leu Asn His Ala Glu Glu Ile
25 30 35aag caa gaa gaa ccc gct ttt gct gtc aag cag cgg gtc att gat att 259Lys Gln Glu Glu Pro Ala Phe Ala Val Lys Gln Arg Val Ile Asp Ile
40 45 50tac tcc aag cag ggt ttt tct tcc att gca ccg gat gac att gcc cca 307Tyr Ser Lys Gln Gly Phe Ser Ser Ile Ala Pro Asp Asp lle Ala Pro
55 60 65cgc ttt aag tgg ttg ggc att tac acc cag cgt aag cag gat ctg ggc 355Arg Phe Lys Trp Leu Gly Ile Tyr Thr Gln Arg Lys Gln Asp Leu Gly70 75 80 85ggt gaa ctg acc ggt cag ctt cct gat gat gag ctg cag gat gag tac 403Gly Glu Leu Thr Gly Gln Leu Pro Asp Asp Glu Leu Gln Asp Glu Tyr
90 95 100ttc atg atg cgt gtg cgt ttt gat ggc gga ctg gct tcc cct gag cgc 451Phe Met Met Arg Val Arg Phe Asp Gly Gly Leu Ala Ser Pro Glu Arg
105 110 115ctg cgt gcc gtg ggt gaa att tct agg gat tat gct cgt tcc acc gcg 499Leu Arg Ala Val Gly Glu Ile Ser Arg Asp Tyr Ala Arg Ser Thr Ala
120 125 130gac ttc acc gac cgc cag aac att cag ctg cac tgg att cgt att gaa 547Asp Phe Thr Asp Arg Gln Asn Ile Gln Leu His Trp Ile Arg Ile Glu
135 140 145gat gtg cct gcg atc tgg gag aag cta gaa acc gtc gga ctg tcc acc 595Asp Val Pro Ala Ile Trp Glu Lys Leu Glu Thr Val Gly Leu Ser Thr150 155 160 165atg ctt ggt tgc ggt gac gtt cca cgt gtt atc ttg ggc tcc cca gtt 643Met Leu Gly Cys Gly Asp Val Pro Arg Val Ile Leu Gly Ser Pro Val
170 175 180tct ggc gta gct gct gaa gag ctg atc gat gcc acc ccg gct atc gat 691Ser Gly Val Ala Ala Glu Glu Leu Ile Asp Ala Thr Pro Ala Ile Asp
185 190 195gcg att cgt gag cgc tac cta gac aag gaa gag ttc cac aac ctt cct 739Ala Ile Arg Glu Arg Tyr Leu Asp Lys Glu Glu Phe His Asn Leu Pro
200 205 210cgt aag ttt aag act gct atc act ggc aac cag cgc cag gat gtt acc 787Arg Lys Phe Lys Thr Ala Ile Thr Gly Asn Gln Arg Gln Asp Val Thr
215 220 225cac gaa atc cag gac gtt tcc ttc gtt cct tcg att cac cca gaa ttc 835His Glu Ile Gln Asp Val Ser Phe Val Pro Ser Ile His Pro Glu Phe230 235 240 245ggc cca gga ttt gag tgc ttt gtg ggc ggt ggc ctg tcc acc aac cca 883Gly Pro Gly Phe Glu Cys Phe Val Gly Gly Gly Leu Ser Thr Asn Pro
250 255 260atg ctt gct cag cca ctt ggt tct tgg att cca ctt gat gag gtt cca 931Met Leu Ala Gln Pro Leu Gly Ser Trp Ile Pro Leu Asp Glu Val Pro
265 270 275gaa gtg tgg gct ggc gtc gcc gga att ttc cgc gac tac ggc ttc cga 979Glu Val Trp Ala Gly Val Ala Gly Ile Phe Arg Asp Tyr Gly Phe Arg
280 285 290cgc ctg cgt aac cgt gct cgc ctc aag ttc ttg gtg gca cag tgg ggt 1027Arg Leu Arg Asn Arg Ala Arg Leu Lys Phe Leu Val Ala Gln Trp Gly
295 300 305att gag aag ttc cgt gaa gtt ctt gag acc gaa tac ctc gag cgc aag 1075Ile Glu Lys Phe Arg Glu Val Leu Glu Thr Glu Tyr Leu Glu Arg Lys310 315 320 325ctg atc gat ggc cca gtt gtt acc acc aac cct ggc tac cgt gac cac 1123Leu Ile Asp Gly Pro Val Val Thr Thr Asn Pro Gly Tyr Arg Asp His
330 335 340att ggc att cac cca caa aag gac ggc aag ttc tac ctc ggt gtg aag 1171Ile Gly Ile His Pro Gln Lys Asp Gly Lys Phe Tyr Leu Gly Val Lys
345 350 355cca acc gtt gga cac acc acc ggt gag cag ctc att gcc att gct gat 1219Pro Thr Val Gly His Thr Thr Gly Glu Gln Leu Ile Ala Ile Ala Asp
360 365 370gtt gca gaa aag cac ggc atc acc agg att cgt acc acg gcg gaa aag 1267Val Ala Glu Lys His Gly Ile Thr Arg Ile Arg Thr Thr Ala Glu Lys
375 380 385gaa ctg ctc ttc ctc gat att gag aga aag aac ctt act acc gtt gca 1315Glu Leu Leu Phe Leu Asp Ile Glu Arg Lys Asn Leu Thr Thr Val Ala390 395 400 405cgc gac ctg gat gaa atc gga ctg tac tct tca cct tcc gag ttc cgc 1363Arg Asp Leu Asp Glu Ile Gly Leu Tyr Ser Ser Pro Ser Glu Phe Arg
410 415 420cgc ggc atc att tcc tgc acc ggc ttg gag ttc tgc aag ctt gcg cac 1411Arg Gly Ile Ile Ser Cys Thr Gly Leu Glu Phe Cys Lys Leu Ala His
425 430 435gca acc acc aag tca cga gca att gag ctt gtc gac gaa ctg gaa gag 1459Ala Thr Thr Lys Ser Arg Ala Ile Glu Leu Val Asp Glu Leu Glu Glu
440 445 450cgc ctc ggc gat ttg gat gtt ccc atc aag att gca ctg aac ggt tgc 1507Arg Leu Gly Asp Leu Asp Val Pro Ile Lys Ile Ala Leu Asn Gly Cys
455 460 465cct aac tct tgt gca cgc acc cag gtt tcc gac atc gga ttc aag gga 1555Pro Asn Ser Cys Ala Arg Thr Gln Val Ser Asp Ile Gly Phe Lys Gly470 475 480 485cag acc gtc act gat gct gac ggc aac cgc gtt gaa ggt ttc cag gtt 1603Gln Thr Val Thr Asp Ala Asp Gly Asn Arg Val Glu Gly Phe Gln Val
490 495 500cac ctg ggc ggt tcc atg aac ttg gat cca aac ttc gga cgc aag ctc 1651His Leu Gly Gly Ser Met Asn Leu Asp Pro Asn Phe Gly Arg Lys Leu
505 510 515aag ggc cac aag gtt att gcc gat gaa gtg gga gag tac gtc act cgc 1699Lys Gly His Lys Val Ile Ala Asp Glu Val Gly Glu Tyr Val Thr Arg
520 525 530gtt gtt acc cac ttc aag gaa cag cgc cac gag gac gag cac ttc cgc 1747Val Val Thr His Phe Lys Glu Gln Arg His Glu Asp Glu His Phe Arg
535 540 545gat tgg gtc cag cgg gcc gct gag gaa gat ttg gtg tgagtcttcg 1793Asp Trp Val Gln Arg Ala Ala Glu Glu Asp Leu Val550 555 560gaggaaaccc aat 1806<210>330<211>561<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>330Met Thr Thr Thr Thr Gly Ser Ala Arg Pro Ala Arg Ala Ala Arg Lys1 5 10 15Pro Lys Pro Glu Gly Gln Trp Lys Ile Asp Gly Thr Glu Pro Leu Asn
20 25 30His Ala Glu Glu Ile Lys Gln Glu Glu Pro Ala Phe Ala ValLys Gln
35 40 45Arg Val Ile Asp Ile Tyr Ser Lys Gln Gly Phe Ser Ser Ile Ala Pro
50 55 60Asp Asp Ile Ala Pro Arg Phe Lys Trp Leu Gly Ile Tyr Thr Gln Arg65 70 75 80Lys Gln Asp Leu Gly Gly Glu Leu Thr Gly Gln Leu Pro Asp Asp Glu
85 90 95Leu Gln Asp Glu Tyr Phe Met Met Arg Val Arg Phe Asp Gly Gly Leu
100 105 110Ala Ser Pro Glu Arg Leu Arg Ala Val Gly Glu Ile Ser Arg Asp Tyr
115 120 125Ala Arg Ser Thr Ala Asp Phe Thr Asp Arg Gln Asn Ile Gln Leu His
130 135 140Trp Ile Arg Ile Glu Asp Val Pro Ala Ile Trp Glu Lys Leu Glu Thr145 150 155 160Val Gly Leu Ser Thr Met Leu Gly Cys Gly Asp Val Pro Arg Val Ile
165 170 175Leu Gly Ser Pro Val Ser Gly Val Ala Ala Glu Glu Leu Ile Asp Ala
180 185 190Thr Pro Ala Ile Asp Ala Ile Arg Glu Arg Tyr Leu Asp Lys Glu Glu
195 200 205Phe His Asn Leu Pro Arg Lys Phe Lys Thr Ala Ile Thr Gly Asn Gln
210 215 220Arg Gln Asp Val Thr His Glu Ile Gln Asp Val Ser Phe Val Pro Ser225 230 235 240Ile His Pro Glu Phe Gly Pro Gly Phe Glu Cys Phe Val Gly Gly Gly
245 250 255Leu Ser Thr Asn Pro Met Leu Ala Gln Pro Leu Gly Ser Trp Ile Pro
260 265 270Leu Asp Glu Val Pro Glu Val Trp Ala Gly Val Ala Gly Ile Phe Arg
275 280 285Asp Tyr Gly Phe Arg Arg Leu Arg Asn Arg Ala Arg Leu Lys Phe Leu
290 295 300Val Ala Gln Trp Gly Ile Glu Lys Phe Arg Glu Val Leu Glu Thr Glu305 310 315 320Tyr Leu Glu Arg Lys Leu Ile Asp Gly Pro Val Val Thr Thr Asn Pro
325 330 335Gly Tyr Arg Asp His Ile Gly Ile His Pro Gln Lys Asp Gly Lys Phe
340 345 350Tyr Leu Gly Val Lys Pro Thr Val Gly His Thr Thr Gly Glu Gln Leu
355 360 365Ile Ala Ile Ala Asp Val Ala Glu Lys His Gly Ile Thr Arg Ile Arg
370 375 380Thr Thr Ala Glu Lys Glu Leu Leu Phe Leu Asp Ile Glu Arg Lys Asn385 390 395 400Leu Thr Thr Val Ala Arg Asp Leu Asp Glu Ile Gly Leu Tyr Ser Ser
405 410 415Pro Ser Glu Phe Arg Arg Gly Ile Ile Ser Cys Thr Gly Leu Glu Phe
420 425 430Cys Lys Leu Ala His Ala Thr Thr Lys Ser Arg Ala Ile Glu Leu Val
435 440 445Asp Glu Leu Glu Glu Arg Leu Gly Asp Leu Asp Val Pro Ile Lys Ile
450 455 460Ala Leu Asn Gly Cys Pro Asn Ser Cys Ala Arg Thr Gln Val Ser Asp465 470 475 480Ile Gly Phe Lys Gly Gln Thr Val Thr Asp Ala Asp Gly Asn Arg Val
485 490 495Glu Gly Phe Gln Val His Leu Gly Gly Ser Met Asn Leu Asp Pro Asn
500 505 510Phe Gly Arg Lys Leu Lys Gly His Lys Val Ile Ala Asp Glu Val Gly
515 520 525Glu Tyr Val Thr Arg Val Val Thr His Phe Lys Glu Gln Arg His Glu
530 535 540Asp Glu His Phe Arg Asp Trp Val Gln Arg Ala Ala Glu Glu Asp Leu545 550 555 560Val<210>331<211>373<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(373)<223>RXN03131<400>331aacgcggctc gtgaagatta cctgaaaaac caagccctcc agcgtccgct gctcggctaa 60accagttggc taaaccaaaa cgtatttaag gagaaacacc atg acc act tct gta 115
Met Thr Thr Ser Val
1 5cct gca tcc acc aaa gct tta tct gtg gct ggc gaa aac cca ggc ctg 163Pro Ala Ser Thr Lys Ala Leu Ser Val Ala Gly Glu Asn Pro Gly Leu
10 15 20cgc atc ggc acc gca cct gac tcg tgg ggc gtg tgg ttc cca gag gat 211Arg Ile Gly Thr Ala Pro Asp Ser Trp Gly Val Trp Phe Pro Glu Asp
25 30 35cca aag cag atc cct tgg gag cgt ttt ctc tac gag gtc gtg aaa gct 259Pro Lys Gln Ile Pro Trp Glu Arg Phe Leu Tyr Glu Val Val Lys Ala
40 45 50ggc tac acc tgg atc gag ctt ggc cca tac ggc tac ctg cca acc gat 307Gly Tyr Thr Trp Ile Glu Leu Gly Pro Tyr Gly Tyr Leu Pro Thr Asp
55 60 65gcc aac cag ctt gaa gat gaa ctg ggc aag cgc ggc ctg aag ctg tcc 355Ala Asn Gln Leu Glu Asp Glu Leu Gly Lys Arg Gly Leu Lys Leu Ser70 75 80 85gct ggc acc gag ttc acc 373Ala Gly Thr Glu Phe Thr
90<210>332<211>91<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>332Met Thr Thr Ser Val Pro Ala Ser Thr Lys Ala Leu Ser Val Ala Gly1 5 10 15Glu Asn Pro Gly Leu Arg Ile Gly Thr Ala Pro Asp Ser Trp Gly Val
20 25 30Trp Phe Pro Glu Asp Pro Lys Gln Ile Pro Trp Glu Arg Phe Leu Tyr
35 40 45Glu Val Val Lys Ala Gly Tyr Thr Trp Ile Glu Leu Gly Pro Tyr Gly
50 55 60Tyr Leu Pro Thr Asp Ala Asn Gln Leu Glu Asp Glu Leu Gly Lys Arg65 70 75 80Gly Leu Lys Leu Ser Ala Gly Thr Glu Phe Thr
85 90<210>333<211>549<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(526)<223>RXS00153<400>333cctcgatttg agtaaagagg acgtcctcgc ctgatttttc gggtgtgttt ttgcgtggcg 60agccctgcct ggcccttcca aattatgtag ggtggcctgc gtg gga gca ata att 115
Val Gly Ala Ile Ile
1 5tgg ttt atc gga gca ttg gtt ctt gct ggc ttg gaa ttg gca gta ggt 163Trp Phe Ile Gly Ala Leu Val Leu Ala Gly Leu Glu Leu Ala Val Gly
10 15 20gag ttc acc tta ttg atg ctc ggc ggt gca gct ttg gca acc gcc ggc 211Glu Phe Thr Leu Leu Met Leu Gly Gly Ala Ala Leu Ala Thr Ala Gly
25 30 35gtg gca ctc atc ggt gtc cca gta tgg gct gaa ttt gtc acc ttc gcg 259Val Ala Leu Ile Gly Val Pro Val Trp Ala Glu Phe Val Thr Phe Ala
40 45 50gtg gcc tca gct gct cta ctg atg ttc att agg ccg gcc att aga aag 307Val Ala Ser Ala Ala Leu Leu Met Phe Ile Arg Pro Ala Ile Arg Lys
55 60 65cgt ctg ctg aaa cca aag gtt ctg gac tct tca cca cga gca ctt gtt 355Arg Leu Leu Lys Pro Lys Val Leu Asp Ser Ser Pro Arg Ala Leu Val70 75 80 85ggc cac cgt gct gaa gtg ctc gaa gat gtc gga gcg acc agc ggg cag 403Gly His Arg Ala Glu Val Leu Glu Asp Val Gly Ala Thr Ser Gly Gln
90 95 100gtc cgc ctg gat ggt tca att tgg tcc gcc cgc agc atg gat ccc aca 451Val Arg Leu Asp Gly Ser Ile Trp Ser Ala Arg Ser Met Asp Pro Thr
105 110 115cac acc ttc gcg gaa ggt gaa att gtc agt gtc att gat atc caa ggc 499His Thr Phe Ala Glu Gly Glu Ile Val Ser Val Ile Asp Ile Gln Gly
120 125 130acg acc gcg att gta tgg aaa gaa gcc taaattttta acaatcaaat 546Thr Thr Ala Ile Val Trp Lys Glu Ala
135 140agt 549<210> 334<211>142<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>334Val Gly Ala Ile Ile Trp Phe Ile Gly Ala Leu Val Leu Ala Gly Leu1 5 10 15Glu Leu Ala Val Gly Glu Phe Thr Leu Leu Met Leu Gly Gly Ala Ala
20 25 30Leu Ala Thr Ala Gly Val Ala Leu Ile Gly Val Pro Val Trp Ala Glu
35 40 45Phe Val Thr Phe Ala Val Ala Ser Ala Ala Leu Leu Met Phe Ile Arg
50 55 60Pro Ala Ile Arg Lys Arg Leu Leu Lys Pro Lys Val Leu Asp Ser Ser65 70 75 80Pro Arg Ala Leu Val Gly His Arg Ala Glu Val Leu Glu Asp Val Gly
85 90 95Ala Thr Ser Gly Gln Val Arg Leu Asp Gly Ser Ile Trp Ser Ala Arg
100 105 110Ser Met Asp Pro Thr His Thr Phe Ala Glu Gly Glu Ile Val Ser Val
115 120 125Ile Asp Ile Gln Gly Thr Thr Ala Ile Val Trp Lys Glu Ala
130 135 140<210>335<211>509<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(486)<223>RXN01716<400>335gaa gtc act cct gag gga ttc aaa gag atc acc cgt gaa aac acc atc 48Glu Val Thr Pro Glu Gly Phe Lys Glu Ile Thr Arg Glu Asn Thr Ile1 5 10 15gtt cgc ctg ggc aaa ggc gtc gac gcc acc ggt cag cta gac ccc gag 96Val Arg Leu Gly Lys Gly Val Asp Ala Thr Gly Gln Leu Asp Pro Glu
20 25 30gca atc gag cgc act cgt gtc gct ttg gaa aac tac gtt gaa ctc atg 144Ala Ile Glu Arg Thr Arg Val Ala Leu Glu Asn Tyr Val Glu Leu Met
35 40 45gaa acc cat ggg gta gag gcc gta cga atg gtt gcc acc tcc gca acc 192Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr
50 55 60cgc gat gcg tcc aac cgc gat gaa ttc ttt tcg atg acc cgc cag ctt 240Arg Asp Ala Ser Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu65 70 75 80ctg tcc aag atc cgt cct gga tac caa gct gaa gta att tcc ggc gaa 288Leu Ser Lys Ile Arg Pro Gly Tyr Gln Ala Glu Val Ile Ser Gly Glu
85 90 95gag gaa gct ctg ctg tcc ttc cga ggt gca atc gtt gac ctg cct gaa 336Glu Glu Ala Leu Leu Ser Phe Arg Gly Ala Ile Val Asp Leu Pro Glu
100 105 110gac caa ggt cct ttc tgt gtt atc gac ctt ggc ggt gga tcc act gag 384Asp Gln Gly Pro Phe Cys Val Ile Asp Leu Gly Gly Gly Ser Thr Glu
115 120 125ttc atc gtt ggc acc tac gac ggt gaa atc cta ggc tcc cac tca acc 432Phe Ile Val Gly Thr Tyr Asp Gly Glu Ile Leu Gly Ser His Ser Thr
130 135 140caa atg gga tgc gtg cgc ctg acc gaa cga atc atg cgc agc gac cca 480Gln Met Gly Cys Val Arg Leu Thr Glu Arg Ile Met Arg Ser Asp Pro145 150 155 160ccc gac tgaaaccgaa gtggaaatcg ccc 509Pro Asp<210>336<211>162<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>336Glu Val Thr Pro Glu Gly Phe Lys Glu Ile Thr Arg Glu Asn Thr Ile1 5 10 15Val Arg Leu Gly Lys Gly Val Asp Ala Thr Gly Gln Leu Asp Pro Glu
20 25 30Ala Ile Glu Arg Thr Arg Val Ala Leu Glu Asn Tyr Val Glu Leu Met
35 40 45Glu Thr His Gly Val Glu Ala Val Arg Met Val Ala Thr Ser Ala Thr
50 55 60Arg Asp Ala Ser Asn Arg Asp Glu Phe Phe Ser Met Thr Arg Gln Leu65 70 75 80Leu Ser Lys Ile Arg Pro Gly Tyr Gln Ala Glu Val Ile Ser Gly Glu
85 90 95Glu Glu Ala Leu Leu Ser Phe Arg Gly Ala Ile Val Asp Leu Pro Glu
100 105 110Asp Gln Gly Pro Phe Cys Val Ile Asp Leu Gly Gly Gly Ser Thr Glu
115 120 125Phe Ile Val Gly Thr Tyr Asp Gly Glu Ile Leu Gly Ser His Ser Thr
130 135 140Gln Met Gly Cys Val Arg Leu Thr Glu Arg Ile Met Arg Ser Asp Pro145 150 155 160Pro Asp<210>337<211>534<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(511)<223>RXN02972<400>337acctacgacg gtgaaatcct aggctcccac tcaacccaaa tgggatgcgt gcgcctgacc 60gaacgaatca tgcgcagcga cccacccgac tgaaaccgaa gtg gaa atc gcc cgc 115
Val Glu Ile Ala Arg
1 5gac tac gtt gca gaa cgc atc cag gaa gta aaa gcc atc gtc cca att 163Asp Tyr Val Ala Glu Arg Ile Gln Glu Val Lys Ala Ile Val Pro Ile
10 15 20tca aag gca aaa acc ttt gtg gga tgc gca ggc acc ttc acc aca atc 211Ser Lys Ala Lys Thr Phe Val Gly Cys Ala Gly Thr Phe Thr Thr Ile
25 30 35tcc gcc tgg gtg caa ggc cta gaa agc tac gac cgc gac gcg atc cac 259Ser Ala Trp Val Gln Gly Leu Glu Ser Tyr Asp Arg Asp Ala Ile His
40 45 50ctc tct gca ctc aac ttc gat gca ctg cga gtt gtc acc gat gag atc 307Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val Val Thr Asp Glu Ile
55 60 65att tca gaa tca tca tca cag cgc gcc agc aac cca gtt gtt gat cca 355Ile Ser Glu Ser Ser Ser Gln Arg Ala Ser Asn Pro Val Val Asp Pro70 75 80 85ggt cgc gcc gac gtc atc ggt ggc gga tcc gtt gtt gtc caa gca gcg 403Gly Arg Ala Asp Val Ile Gly Gly Gly Ser Val Val Val Gln Ala Ala
90 95 100atc gac tta gcc tcc aaa gaa gcc ggt gta gac tac atc att att tcc 451Ile Asp Leu Ala Ser Lys Glu Ala Gly Val Asp Tyr Ile Ile Ile Ser
105 110 115gaa aaa gac atc ctc gac ggc ctc atc ctt ggc ctg gta gaa gcc gac 499Glu Lys Asp Ile Leu Asp Gly Leu Ile Leu Gly Leu Val Glu Ala Asp
120 125 130tct ttg aag aaa taggacccta gttttaaacc act 534Ser Leu Lys Lys
135<210>338<211>137<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>338Val Glu Ile Ala Arg Asp Tyr Val Ala Glu Arg Ile Gln Glu Val Lys1 5 10 15Ala Ile Val Pro Ile Ser Lys Ala Lys Thr Phe Val Gly Cys Ala Gly
20 25 30Thr Phe Thr Thr Ile Ser Ala Trp Val Gln Gly Leu Glu Ser Tyr Asp
35 40 45Arg Asp Ala Ile His Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val
50 55 60Val Thr Asp Glu Ile Ile Ser Glu Ser Ser Ser Gln Arg Ala Ser Asn65 70 75 80Pro Val Val Asp Pro Gly Arg Ala Asp Val Ile Gly Gly Gly Ser Val
85 90 95Val Val Gln Ala Ala Ile Asp Leu Ala Ser Lys Glu Ala Gly Val Asp
100 105 110Tyr Ile Ile Ile Ser Glu Lys Asp Ile Leu Asp Gly Leu Ile Leu Gly
115 120 125Leu Val Glu Ala Asp Ser Leu Lys Lys
130 135<210>339<211>1497<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1474)<223>RXN00663<400>339ctgaacgatt ggtgaccggc tcatgaaaac ttgacgagtc cccggtattc gccagcggtg 60actactaccg tgggcgacaa gcccacttag aggaggactt gtg aca acc acc tat 115
Val Thr Thr Thr Tyr
1 5cca gat ttc ctt gga aat tct tcg ctc caa aca gat acg gag cac tgg 163Pro Asp Phe Leu Gly Asn Ser Ser Leu Gln Thr Asp Thr Glu His Trp
10 15 20gaa atg gaa gga ggt gcg cag gaa gtc tct gtt act tat gtt ttg gac 211Glu Met Glu Gly Gly Ala Gln Glu Val Ser Val Thr Tyr Val Leu Asp
25 30 35acg tca gtg ttg ctg tct gat ccg ttg tcg ttg aca cgg ttc gcg gag 259Thr Ser Val Leu Leu Ser Asp Pro Leu Ser Leu Thr Arg Phe Ala Glu
40 45 50cac gat gta gtt ctg cca att gtt gta att acg gaa tta gaa gcc aag 307His Asp Val Val Leu Pro Ile Val Val Ile Thr Glu Leu Glu Ala Lys
55 60 65cgt cat cac ccg gac ctt ggc ttt ttt gct cgc caa gcg ctt cgg atg 355Arg His His Pro Asp Leu Gly Phe Phe Ala Arg Gln Ala Leu Arg Met70 75 80 85ctg gat gag ctg cgt gag atc cat ggg gat ttg tcc aag cca ctg cca 403Leu Asp Glu Leu Arg Glu Ile His Gly Asp Leu Ser Lys Pro Leu Pro
90 95 100att ggc gat gaa ggc gga cac atc cat gtt gag ctg aat cac caa aac 451Ile Gly Asp Glu Gly Gly His Ile His Val Glu Leu Asn His Gln Asn
105 110 115acg ggg tcc ttg ccc gtg gga ttc cgc ctt ggt gac aat gac acc cgc 499Thr Gly Ser Leu Pro Val Gly Phe Arg Leu Gly Asp Asn Asp Thr Arg
120 125 130atc ctt gca gtg gcc aag aat ctg cag gaa gag ggc cac aat gtg gtt 547Ile Leu Ala Val Ala Lys Asn Leu Gln Glu Glu Gly His Asn Val Val
135 140 145ctg gtg tcg aag gac ctg ccg atg cgg att aag gcg tcg gca agc gga 595Leu Val Ser Lys Asp Leu Pro Met Arg Ile Lys Ala Ser Ala Ser Gly150 155 160 165atc gcc gca cag gaa tac cgc gct gcc ctg gcg cgc gac cgt ggt tac 643Ile Ala Ala Gln Glu Tyr Arg Ala Ala Leu Ala Arg Asp Arg Gly Tyr
170 175 180acc ggc atg acc cac gcc aat atc acc gat gac cag ctc agc gag ctc 691Thr Gly Met Thr His Ala Asn Ile Thr Asp Asp Gln Leu Ser Glu Leu
185 190 195tac gac acc ggc gag gtg cgc att gag gag ctc gaa aag ctg ccc gtc 739Tyr Asp Thr Gly Glu Val Arg Ile Glu Glu Leu Glu Lys Leu Pro Val
200 205 210aac cac ggc ttc acc ctc aaa tcc aac agc ggt tcg gcg ctt ggt cgt 787Asn His Gly Phe Thr Leu Lys Ser Asn Ser Gly Ser Ala Leu Gly Arg
215 220 225atg aat tcc gac aag atc atc gag ctt gtc ccc ggc gac cag cag gta 835Met Asn Ser Asp Lys Ile Ile Glu Leu Val Pro Gly Asp Gln Gln Val230 235 240 245ttc ggt atc agc ggg cgt agc gct gag cag cgg gtt gcc att gat ttg 883Phe Gly Ile Ser Gly Arg Ser Ala Glu Gln Arg Val Ala Ile Asp Leu
250 255 260ctt aac gac gac gcc gtc ggc atc gta tcc atc ggc ggc ccc gcg ggt 931Leu Asn Asp Asp Ala Val Gly Ile Val Ser Ile Gly Gly Pro Ala Gly
265 270 275aca ggt aaa agc gca ctc gca ctg tgt gcc ggc ctg gaa gct gtg atg 979Thr Gly Lys Ser Ala Leu Ala Leu Cys Ala Gly Leu Glu Ala Val Met
280 285 290gag cgt cgc att cag cgc aag att atc gtg ttc cgc cca ctc ttt gcc 1027Glu Arg Arg Ile Gln Arg Lys Ile Ile Val Phe Arg Pro Leu Phe Ala
295 300 305gtt ggc gga cag gaa ctt ggc tac ctg cct ggc gac caa gaa gaa aaa 1075Val Gly Gly Gln Glu Leu Gly Tyr Leu Pro Gly Asp Gln Glu Glu Lys310 315 320 325atg ggg cct tgg gcg caa gcg gtt ttt gac acc cta agc tcc atg gtc 1123Met Gly Pro Trp Ala Gln Ala Val Phe Asp Thr Leu Ser Ser Met Val
330 335 340agc caa aac atc atc gat gaa gcc ctc tcc cgc ggc ctc atc gaa gtt 1171Ser Gln Asn Ile Ile Asp Glu Ala Leu Ser Arg Gly Leu Ile Glu Val
345 350 355ctc cca ctt act cac atc cgc gga cgc tca ctc cac gat gct ttc gtc 1219Leu Pro Leu Thr His Ile Arg Gly Arg Ser Leu His Asp Ala Phe Val
360 365 370atc gtc gac gag gcc caa tcc cta gaa cgc aac gtg ttg ctc acc atg 1267Ile Val Asp Glu Ala Gln Ser Leu Glu Arg Asn Val Leu Leu Thr Met
375 380 385ctg tct cgc atc ggc cag aat tcc cga gta gtt ctc acc cat gac gta 1315Leu Ser Arg Ile Gly Gln Asn Ser Arg Val Val Leu Thr His Asp Val390 395 400 405gcg cag cgc gac aac ctg cgc gtt ggt cgc tac gac ggc atc gtc tct 1363Ala Gln Arg Asp Asn Leu Arg Val Gly Arg Tyr Asp Gly Ile Val Ser
410 415 420gtg gtg gaa gca ctc aag gat cac gaa ctg ttt ggc cac atc acg ttg 1411Val Val Glu Ala Leu Lys Asp His Glu Leu Phe Gly His Ile Thr Leu
425 430 435cag cgt tcc gaa cgc tcc cga atc gct gag ttg gtc acc caa gtt ttg 1459Gln Arg Ser Glu Arg Ser Arg Ile Ala Glu Leu Val Thr Gln Val Leu
440 445 450gat gcg ccg tct ctg tagtcgcgca gtctgtggcg att 1497Asp Ala Pro Ser Leu
455<210>340<211>458<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>340Val Thr Thr Thr Tyr Pro Asp Phe Leu Gly Asn Ser Ser Leu Gln Thr1 5 10 15Asp Thr Glu His Trp Glu Met Glu Gly Gly Ala Gln Glu Val Ser Val
20 25 30Thr Tyr Val Leu Asp Thr Ser Val Leu Leu Ser Asp Pro Leu Ser Leu
35 40 45Thr Arg Phe Ala Glu His Asp Val Val Leu Pro Ile Val Val Ile Thr
50 55 60Glu Leu Glu Ala Lys Arg His His Pro Asp Leu Gly Phe Phe Ala Arg65 70 75 80Gln Ala Leu Arg Met Leu Asp Glu Leu Arg Glu Ile His Gly Asp Leu
85 90 95Ser Lys Pro Leu Pro Ile Gly Asp Glu Gly Gly His Ile His Val Glu
100 105 110Leu Asn His Gln Asn Thr Gly Ser Leu Pro Val Gly Phe Arg Leu Gly
115 120 125Asp Asn Asp Thr Arg Ile Leu Ala Val Ala Lys Asn Leu Gln Glu Glu
130 135 140Gly His Asn Val Val Leu Val Ser Lys Asp Leu Pro Met Arg Ile Lys145 150 155 160Ala Ser Ala Ser Gly Ile Ala Ala Gln Glu Tyr Arg Ala Ala Leu Ala
165 170 175Arg Asp Arg Gly Tyr Thr Gly Met Thr His Ala Asn Ile Thr Asp Asp
180 185 190Gln Leu Ser Glu Leu Tyr Asp Thr Gly Glu Val Arg Ile Glu Glu Leu
195 200 205Glu Lys Leu Pro Val Asn His Gly Phe Thr Leu Lys Ser Asn Ser Gly
210 215 220Ser Ala Leu Gly Arg Met Asn Ser Asp Lys Ile Ile Glu Leu Val Pro225 230 235 240Gly Asp Gln Gln Val Phe Gly Ile Ser Gly Arg Ser Ala Glu Gln Arg
245 250 255Val Ala Ile Asp Leu Leu Asn Asp Asp Ala Val Gly Ile Val Ser Ile
260 265 270Gly Gly Pro Ala Gly Thr Gly Lys Ser Ala Leu Ala Leu Cys Ala Gly
275 280 285Leu Glu Ala Val Met Glu Arg Arg Ile Gln Arg Lys Ile Ile Val Phe
290 295 300Arg Pro Leu Phe Ala Val Gly Gly Gln Glu Leu Gly Tyr Leu Pro Gly305 310 315 320Asp Gln Glu Glu Lys Met Gly Pro Trp Ala Gln Ala Val Phe Asp Thr
325 330 335Leu Ser Ser Met Val Ser Gln Asn Ile Ile Asp Glu Ala Leu Ser Arg
340 345 350Gly Leu Ile Glu Val Leu Pro Leu Thr His Ile Arg Gly Arg Ser Leu
355 360 365His Asp Ala Phe Val Ile Val Asp Glu Ala Gln Ser Leu Glu Arg Asn
370 375 380Val Leu Leu Thr Met Leu Ser Arg Ile Gly Gln Asn Ser Arg Val Val385 390 395 400Leu Thr His Asp Val Ala Gln Arg Asp Asn Leu Arg Val Gly Arg Tyr
405 410 415Asp Gly Ile Val Ser Val Val Glu Ala Leu Lys Asp His Glu Leu Phe
420 425 430Gly His Ile Thr Leu Gln Arg Ser Glu Arg Ser Arg Ile Ala Glu Leu
435 440 445Val Thr Gln Val Leu Asp Ala Pro Ser Leu
450 455<210>341<211>1248<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1225)<223>RXN00778<400>341aggtcttagg tttttaagtc gtgagcaatc cggagggaaa ctagcccgcc tacaggatct 60gctcagacga tgtcttcact taaaccggaa aggcttcccc gtg aac ctc act ctt 115
Val Asn Leu Thr Leu
1 5aag cgc tcc atc gcc ctt gtg ggc gca gtt act gca ggc tcc ttc gct 163Lys Arg Ser Ile Ala Leu Val Gly Ala Val Thr Ala Gly Ser Phe Ala
10 15 20ctt gta gct tgc tcc gac tcc aat gag tct gat tcc acc tcc tca tct 211Leu Val Ala Cys Ser Asp Ser Asn Glu Ser Asp Ser Thr Ser Ser Ser
25 30 35gca gct tcc acc ggt tct tcc gat gct gca tcc att gag ggc ctt tcc 259Ala Ala Ser Thr Gly Ser Ser Asp Ala Ala Ser Ile Glu Gly Leu Ser
40 45 50ggt gtt acc ggt cag ctc gtt gct gaa ggt gca tct tcc cag cag tcc 307Gly Val Thr Gly Gln Leu Val Ala Glu Gly Ala Ser Ser Gln Gln Ser
55 60 65gca atg gac tac ttt ggt atc cgt tac tcc gag gct gtc agc ggt gca 355Ala Met Asp Tyr Phe Gly Ile Arg Tyr Ser Glu Ala Val Ser Gly Ala70 75 80 85tct ctg gct tac acc cct tca ggt tcc ggt tcc ggc cgc acc aac ttc 403Ser Leu Ala Tyr Thr Pro Ser Gly Ser Gly Ser Gly Arg Thr Asn Phe
90 95 100gct gca ggc cag gtt gct ttc ggt ggc tcc gac tcc gca atg aag gac 451Ala Ala Gly Gln Val Ala Phe Gly Gly Ser Asp Ser Ala Met Lys Asp
105 110 115gac cag gct gca gaa gca gaa gca cgt tgc aac ggc aac gaa gca tgg 499Asp Gln Ala Ala Glu Ala Glu Ala Arg Cys Asn Gly Asn Glu Ala Trp
120 125 130cac ctg cca ttc gtt atc ggc cca gtt gca gtt gct tac aac ctg cct 547His Leu Pro Phe Val Ile Gly Pro Val Ala Val Ala Tyr Asn Leu Pro
135 140 145ggc gtt gac acc ctg aac ctg gac acc aac atc atc gct cag atc ttc 595Gly Val Asp Thr Leu Asn Leu Asp Thr Asn Ile Ile Ala Gln Ile Phe150 155 160 165aag ggc gag atc acc aag tgg aac gac gaa gca atc gct tcc cag aac 643Lys Gly Glu Ile Thr Lys Trp Asn Asp Glu Ala Ile Ala Ser Gln Asn
170 175 180gag ggc acc gac ctc cca gac cag gac atc tcc gtt ctg tac cgt tcc 691Glu Gly Thr Asp Leu Pro Asp Gln Asp Ile Ser Val Leu Tyr Arg Ser
185 190 195gaa gag tcc ggt acc tcc gac aac ttc cag aag ttc ctc gga gct tcc 739Glu Glu Ser Gly Thr Ser Asp Asn Phe Gln Lys Phe Leu Gly Ala Ser
200 205 210acc gac atc tgg gag acc gaa ggc cag cag ttc cca acc gag gtt ggc 787Thr Asp Ile Trp Glu Thr Glu Gly Gln Gln Phe Pro Thr Glu Val Gly
215 220 225tcc ggt gcg cag ggc tcc aac ggt gta gct tct gag gct tcc aac atc 835Ser Gly Ala Gln Gly Ser Asn Gly Val Ala Ser Glu Ala Ser Asn Ile230 235 240 245gag ggt gca atc acc tac gtt gaa gct ggt ttc gct aac cag tcc ggc 883Glu Gly Ala Ile Thr Tyr Val Glu Ala Gly Phe Ala Asn Gln Ser Gly
250 255 260ctg ggc gtt gca aac atc gac ttc ggt tcc ggc cca gtt gaa ctc aac 931Leu Gly Val Ala Asn Ile Asp Phe Gly Ser Gly Pro Val Glu Leu Asn
265 270 275gct gag tcc gtt ggc gtt gca ctt ggt gca ctc gac ttc ctg act gag 979Ala Glu Ser Val Gly Val Ala Leu Gly Ala Leu Asp Phe Leu Thr Glu
280 285 290ggc cac aac atg gtt gtt gac acc gac gct atg ttc gca atg aac gaa 1027Gly His Asn Met Val Val Asp Thr Asp Ala Met Phe Ala Met Asn Glu
295 300 305gcc ggt gct tac cca ctg atc ctc acc acc tac gaa atc gtc tgc tcc 1075Ala Gly Ala Tyr Pro Leu Ile Leu Thr Thr Tyr Glu Ile Val Cys Ser310 315 320 325gca ggc tac gac gag acc acc cgc gac cag gtc aag gac ttc ctg acc 1123Ala Gly Tyr Asp Glu Thr Thr Arg Asp Gln Val Lys Asp Phe Leu Thr
330 335 340gtt gca ctg gac tcc cag gat gac cag ctc gag gct ctc ggc tac atc 1171Val Ala Leu Asp Ser Gln Asp Asp Gln Leu Glu Ala Leu Gly Tyr Ile
345 350 355cca gtt acc ggc gag cac tac gat cgc ctc gtt gca gca gtt gaa gca 1219Pro Val Thr Gly Glu His Tyr Asp Arg Leu Val Ala Ala Val Glu Ala
360 365 370att cag taataaaccg ctgccgtagc ttc 1248Ile Gln
375<210>342<211>375<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>342Val Asn Leu Thr Leu Lys Arg Ser Ile Ala Leu Val Gly Ala Val Thr1 5 10 15Ala Gly Ser Phe Ala Leu Val Ala Cys Ser Asp Ser Asn Glu Ser Asp
20 25 30Ser Thr Ser Ser Ser Ala Ala Ser Thr Gly Ser Ser Asp Ala Ala Ser
35 40 45Ile Glu Gly Leu Ser Gly Val Thr Gly Gln Leu Val Ala Glu Gly Ala
50 55 60Ser Ser Gln Gln Ser Ala Met Asp Tyr Phe Gly Ile Arg Tyr Ser Glu65 70 75 80Ala Val Ser Gly Ala Ser Leu Ala Tyr Thr Pro Ser Gly Ser Gly Ser
85 90 95Gly Arg Thr Asn Phe Ala Ala Gly Gln Val Ala Phe Gly Gly Ser Asp
100 105 110Ser Ala Met Lys Asp Asp Gln Ala Ala Glu Ala Glu Ala Arg Cys Asn
115 120 125Gly Asn Glu Ala Trp His Leu Pro Phe Val Ile Gly Pro Val Ala Val
130 135 140Ala Tyr Asn Leu Pro Gly Val Asp Thr Leu Asn Leu Asp Thr Asn Ile145 150 155 160Ile Ala Gln Ile Phe Lys Gly Glu Ile Thr Lys Trp Asn Asp Glu Ala
165 170 175Ile Ala Ser Gln Asn Glu Gly Thr Asp Leu Pro Asp Gln Asp Ile Ser
180 185 190Val Leu Tyr Arg Ser Glu Glu Ser Gly Thr Ser Asp Asn Phe Gln Lys
195 200 205Phe Leu Gly Ala Ser Thr Asp Ile Trp Glu Thr Glu Gly Gln Gln Phe
210 215 220Pro Thr Glu Val Gly Ser Gly Ala Gln Gly Ser Asn Gly Val Ala Ser225 230 235 240Glu Ala Ser Asn Ile Glu Gly Ala Ile Thr Tyr Val Glu Ala Gly Phe
245 250 255Ala Asn Gln Ser Gly Leu Gly Val Ala Asn Ile Asp Phe Gly Ser Gly
260 265 270Pro Val Glu Leu Asn Ala Glu Ser Val Gly Val Ala Leu Gly Ala Leu
275 280 285Asp Phe Leu Thr Glu Gly His Asn Met Val Val Asp Thr Asp Ala Met
290 295 300Phe Ala Met Asn Glu Ala Gly Ala Tyr Pro Leu Ile Leu Thr Thr Tyr305 310 315 320Glu Ile Val Cys Ser Ala Gly Tyr Asp Glu Thr Thr Arg Asp Gln Val
325 330 335Lys Asp Phe Leu Thr Val Ala Leu Asp Ser Gln Asp Asp Gln Leu Glu
340 345 350Ala Leu Gly Tyr Ile Pro Val Thr Gly Glu His Tyr Asp Arg Leu Val
355 360 365Ala Ala Val Glu Ala Ile Gln
370 375<210>343<211>870<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(847)<223>RXN00250<400>343acaacaccag accaccccaa ccctgaataa acccctattt ttctaaaaag tcacactttg 60ccgtatagaa attcagtcaa ccaagagtac tctgtccacc atg gtt ttt act ctt 115
Met Val Phe Thr Leu
1 5gcg gac tcc gtc tcc cag gtt gcg cta ggt ccg tcc tgg ctg gac cct 163Ala Asp Ser Val Ser Gln Val Ala Leu Gly Pro Ser Trp Leu Asp Pro
10 15 20atg gaa ctt ctt tcc ggc tcc ggc ccg ttc ggt agc ttc att ctt ccg 211Met Glu Leu Leu Ser Gly Ser Gly Pro Phe Gly Ser Phe Ile Leu Pro
25 30 35gcg atg ctt gcc att gtc ttt atc gaa tca ggc cta ctt ttc cca ctt 259Ala Met Leu Ala Ile Val Phe Ile Glu Ser Gly Leu Leu Phe Pro Leu
40 45 50cta cca ggt gat tct ctc ctt ttc acc ggt ggt ctc cta gct aac cag 307Leu Pro Gly Asp Ser Leu Leu Phe Thr Gly Gly Leu Leu Ala Asn Gln
55 60 65gct gac cct ttt gca ccg ctg tgg ctg gtg ctg atc ctc tgc cct atc 355Ala Asp Pro Phe Ala Pro Leu Trp Leu Val Leu Ile Leu Cys Pro Ile70 75 80 85gcc gca att ctt ggc gat cag gtg ggt tac tgg att ggc cac aag ttc 403Ala Ala Ile Leu Gly Asp Gln Val Gly Tyr Trp Ile Gly His Lys Phe
90 95 100cac cct cgc ctg gtc aat cgt ccg gat ggc agg att ttc aag cag gaa 451His Pro Arg Leu Val Asn Arg Pro Asp Gly Arg Ile Phe Lys Gln Glu
105 110 115tac ctc aag cag act gag gat ttc ttt gag aag cat ggc ccc gtg acg 499Tyr Leu Lys Gln Thr Glu Asp Phe Phe Glu Lys His Gly Pro Val Thr
120 125 130atc att ttg tgc cgt ttc gtg ccc atc gtc cgt act tac gca cct ctg 547Ile Ile Leu Cys Arg Phe Val Pro Ile Val Arg Thr Tyr Ala Pro Leu
135 140 145gtc gca ggt atg gct ggc atg cgt tac cgc acg ttc att att tac aac 595Val Ala Gly Met Ala Gly Met Arg Tyr Arg Thr Phe Ile Ile Tyr Asn150 155 160 165atg atc ggt ggc att ttg tgg ggt tcc ggc gtg gtg gct ttg ggt gct 643Met Ile Gly Gly Ile Leu Trp Gly Ser Gly Val Val Ala Leu Gly Ala
170 175 180gcg ttg ggt cag ttc gat ttc gtc cgc aac aat att gat ctg att ttc 691Ala Leu Gly Gln Phe Asp Phe Val Arg Asn Asn Ile Asp Leu Ile Phe
185 190 195ttg ctg atc gtg ttc att tcg gtg gtt cct ggt ttg gtc ggc atg gcc 739Leu Leu Ile Val Phe Ile Ser Val Val Pro Gly Leu Val Gly Met Ala
200 205 210cgc aag ctg gct gac ggc cac aag caa gcc aac acc gag cca caa gaa 787Arg Lys Leu Ala Asp Gly His Lys Gln Ala Asn Thr Glu Pro Gln Glu
215 220 225aac ccc gca gtc cag aca gcc cca gta aaa acc cag gaa gcc cag gaa 835Asn Pro Ala Val Gln Thr Ala Pro Val Lys Thr Gln Glu Ala Gln Glu230 235 240 245gcc ccc cag aac taatctttcc ggtccgccag ttc 870Ala Pro Gln Asn<210>344<211>249<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>344Met Val Phe Thr Leu Ala Asp Ser Val Ser Gln Val Ala Leu Gly Pro1 5 10 15Ser Trp Leu Asp Pro Met Glu Leu Leu Ser Gly Ser Gly Pro Phe Gly
20 25 30Ser Phe Ile Leu Pro Ala Met Leu Ala Ile Val Phe Ile Glu Ser Gly
35 40 45Leu Leu Phe Pro Leu Leu Pro Gly Asp Ser Leu Leu Phe Thr Gly Gly
50 55 60Leu Leu Ala Asn Gln Ala Asp Pro Phe Ala Pro Leu Trp Leu Val Leu65 70 75 80Ile Leu Cys Pro Ile Ala Ala Ile Leu Gly Asp Gln Val Gly Tyr Trp
85 90 95Ile Gly His Lys Phe His Pro Arg Leu Val Asn Arg Pro Asp Gly Arg
100 105 110Ile Phe Lys Gln Glu Tyr Leu Lys Gln Thr Glu Asp Phe Phe Glu Lys
115 120 125His Gly Pro Val Thr Ile Ile Leu Cys Arg Phe Val Pro Ile Val Arg
130 135 140Thr Tyr Ala Pro Leu Val Ala Gly Met Ala Gly Met Arg Tyr Arg Thr145 150 155 160Phe Ile Ile Tyr Asn Met Ile Gly Gly Ile Leu Trp Gly Ser Gly Val
165 170 175Val Ala Leu Gly Ala Ala Leu Gly Gln Phe Asp Phe Val Arg Asn Asn
180 185 190Ile Asp Leu Ile Phe Leu Leu Ile Val Phe Ile Ser Val Val Pro Gly
195 200 205Leu Val Gly Met Ala Arg Lys Leu Ala Asp Gly His Lys Gln Ala Asn
210 215 220Thr Glu Pro Gln Glu Asn Pro Ala Val Gln Thr Ala Pro Val Lys Thr225 230 235 240Gln Glu Ala Gln Glu Ala Pro Gln Asn
245<210>345<211>541<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(541)<223>RXA00072<400>345acggccagga cgatccagtg cacaggccag caccagcaaa gtccacatcg caagcattaa 60aagaatctct cgaaagacac aaaagaggtg agtcgcaaca atg agc ttt caa cta 115
Met Ser Phe Gln Leu
1 5gtt aac gcc ctg aaa aat act ggt tcg gta aaa gat ccc gag atc tca 163Val Asn Ala Leu Lys Asn Thr Gly Ser Val Lys Asp Pro Glu Ile Ser
10 15 20ccc gaa gga cct cgc acg acc aca ccg ttg tca cca gag gta gca aaa 211Pro Glu Gly Pro Arg Thr Thr Thr Pro Leu Ser Pro Glu Val Ala Lys
25 30 35cat aac gag gaa ctc gtc gaa aag cat gct gct gcg ttg tat gac gcc 259His Asn Glu Glu Leu Val Glu Lys His Ala Ala Ala Leu Tyr Asp Ala
40 45 50agc gcg caa gag atc ctg gaa tgg aca gcc gag cac gcg ccg ggc gct 307Ser Ala Gln Glu Ile Leu Glu Trp Thr Ala Glu His Ala Pro Gly Ala
55 60 65att gca gtg acc ttg agc atg gaa aac acc gtg ctg gcg gag ctg gct 355Ile Ala Val Thr Leu Ser Met Glu Asn Thr Val Leu Ala Glu Leu Ala70 75 80 85gcg cgg cac ctg ccg gaa gct gat ttc ctc ttt ttg gac acc ggt tac 403Ala Arg His Leu Pro Glu Ala Asp Phe Leu Phe Leu Asp Thr Gly Tyr
90 95 100cac ttc aag gag acc ctt gaa gtt gcc cgt cag gta gat gag cgc tat 451His Phe Lys Glu Thr Leu Glu Val Ala Arg Gln Val Asp Glu Arg Tyr
105 110 115tcc cag aag ctt gtc acc gcg ctg ccg atc ctc aag cgc acg gag cag 499Ser Gln Lys Leu Val Thr Ala Leu Pro Ile Leu Lys Arg Thr Glu Gln
120 125 130gat tcc att tat ggt ctc aac ctg tac cgc agc aac cca gcg 541Asp Ser Ile Tyr Gly Leu Asn Leu Tyr Arg Ser Asn Pro Ala
135 140 145<210>346<211>147<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>346Met Ser Phe Gln Leu Val Asn Ala Leu Lys Asn Thr Gly Ser Val Lys1 5 10 15Asp Pro Glu Ile Ser Pro Glu Gly Pro Arg Thr Thr Thr Pro Leu Ser
20 25 30Pro Glu Val Ala Lys His Asn Glu Glu Leu Val Glu Lys His Ala Ala
35 40 45Ala Leu Tyr Asp Ala Ser Ala Gln Glu Ile Leu Glu Trp Thr Ala Glu
50 55 60His Ala Pro Gly Ala Ile Ala Val Thr Leu Ser Met Glu Asn Thr Val65 70 75 80Leu Ala Glu Leu Ala Ala Arg His Leu Pro Glu Ala Asp Phe Leu Phe
85 90 95Leu Asp Thr Gly Tyr His Phe Lys Glu Thr Leu Glu Val Ala Arg Gln
100 105 110Val Asp Glu Arg Tyr Ser Gln Lys Leu Val Thr Ala Leu Pro Ile Leu
115 120 125Lys Arg Thr Glu Gln Asp Ser Ile Tyr Gly Leu Asn Leu Tyr Arg Ser
130 135 140Asn Pro Ala145<210>347<211>1299<212>DNA<213>谷氨酸棒杆菌(Corynebacter ium glutamicum)<220><221>CDS<222>(101)..(1276)<223>RXA00793<400>347tcgctggttt ttagatggtt ttcaagccag cgagaccaca ttagtttcac gctggttgaa 60acctttgaga tcaatataga ccgtgtggtc tactcgagga atg agt gaa aac aat 115
Met Ser Glu Asn Asn
1 5ccc act acc ttg cac tgg ttc cta ccc acc tat ggc gat tct cgc gga 163Pro Thr Thr Leu His Trp Phe Leu Pro Thr Tyr Gly Asp Ser Arg Gly
10 15 20atc aca gcc ggc ggg cat ggc ttc ggc ttc cac tcc gga agc cgg aca 211Ile Thr Ala Gly Gly His Gly Phe Gly Phe His Ser Gly Ser Arg Thr
25 30 35gca gac ctc gat tac ctc tcc caa att gcc ctg gcc gct gaa cga aac 259Ala Asp Leu Asp Tyr Leu Ser Gln Ile Ala Leu Ala Ala Glu Arg Asn
40 45 50ggt ttt gaa tcc gtc ctg act ccc act gga ttg tgg tgc gaa gat gcg 307Gly Phe Glu Ser Val Leu Thr Pro Thr Gly Leu Trp Cys Glu Asp Ala
55 60 65tgg atc acc acc gca gcg ctg ctg tct agg aca tca aaa ctg aaa ttc 355Trp Ile Thr Thr Ala Ala Leu Leu Ser Arg Thr Ser Lys Leu Lys Phe70 75 80 85ctc gtt gct att cga cca ggc caa gtt agc ccc acc atc atc gcg cag 403Leu Val Ala Ile Arg Pro Gly Gln Val Ser Pro Thr Ile Ile Ala Gln
90 95 100cag ggt gct gcc ttc cag aaa ttc tca aat aac cgc ctg ctc atc aac 451Gln Gly Ala Ala Phe Gln Lys Phe Ser Asn Asn Arg Leu Leu Ile Asn
105 110 115gtc gtg gtg ggt ggc gaa gac cat gaa cag cgc gct ttc gct gat tat 499Val Val Val Gly Gly Glu Asp His Glu Gln Arg Ala Phe Ala Asp Tyr
120 125 130tct tcc aaa gag gag cgc tac cac aag gct gat gaa acc tta gag atc 547Ser Ser Lys Glu Glu Arg Tyr His Lys Ala Asp Glu Thr Leu Glu Ile
135 140 145atc gat cac cta tgg aac agc gca gaa cct cta aat ttc cag ggt gaa 595Ile Asp His Leu Trp Asn Ser Ala Glu Pro Leu Asn Phe Gln Gly Glu150 155 160 165ttc ctc agt gtg gaa aac gcg gta ttg aag gaa cag ccc gag gtt tcc 643Phe Leu Ser Val Glu Asn Ala Val Leu Lys Glu Gln Pro Glu Val Ser
170 175 180cca ccg att tac ttt ggc gga tcc tca caa ctc ggc atc gaa atc gca 691Pro Pro Ile Tyr Phe Gly Gly Ser Ser Gln Leu Gly Ile Glu Ile Ala
185 190 195gcc caa cat tcc gat gtt tat ctc acc tgg ggt gaa cct gcg gaa aag 739Ala Gln His Ser Asp Val Tyr Leu Thr Trp Gly Glu Pro Ala Glu Lys
200 205 210gta gag gag aag ctt gcc cgg gtg cgc gcc gaa gca gat aag cga aac 787Val Glu Glu Lys Leu Ala Arg Val Arg Ala Glu Ala Asp Lys Arg Asn
215 220 225cgc gaa cta gac tat ggc atc cgc ctg cat gtc att gct cga cca act 835Arg Glu Leu Asp Tyr Gly Ile Arg Leu His Val Ile Ala Arg Pro Thr230 235 240 245gag gat gaa gcc tgg tca gtg gct caa aat ctt ctt gac caa ctt gat 883Glu Asp Glu Ala Trp Ser Val Ala Gln Asn Leu Leu Asp Gln Leu Asp
250 255 260cag gaa gag gtt gcc cgc att cag gaa ggg ctt gcg cgt tct caa tcg 931Gln Glu Glu Val Ala Arg Ile Gln Glu Gly Leu Ala Arg Ser Gln Ser
265 270 275gaa ggt cag cgt cgc atg acg gaa ctt cat gga caa ggg gca gca ttc 979Glu Gly Gln Arg Arg Met Thr Glu Leu His Gly Gln Gly Ala Ala Phe
280 285 290aca gca gga gca gat gct cgc tcc ctt gaa att gca ccg aat ctc tgg 1027Thr Ala Gly Ala Asp Ala Arg Ser Leu Glu Ile Ala Pro Asn Leu Trp
295 300 305gca ggt gtt ggg cta gtc cgc ggt ggc gcc ggc aca gcg ttg gtg ggt 1075Ala Gly Val Gly Leu Val Arg Gly Gly Ala Gly Thr Ala Leu Val Gly310 315 320 325tcc tat gag caa gtc gcg caa gca att ttg cga tac cgc gat att ggt 1123Ser Tyr Glu Gln Val Ala Gln Ala Ile Leu Arg Tyr Arg Asp Ile Gly
330 335 340
ctg agc cac ttc att ttc tcc ggc tat cca cat ttg gag gaa acc tat 1171Leu Ser His Phe Ile Phe Ser Gly Tyr Pro His Leu Glu Glu Thr Tyr
345 350 355cac gtg ggc gaa gga gtg gta cct gag ctc ctc aaa ttg ggt gtt ccg 1219His Val Gly Glu Gly Val Val Pro Glu Leu Leu Lys Leu Gly Val Pro
360 365 370gtg aac aac cat gaa gaa caa cgc aac gac gtg gta gcg act ccg ttt 1267Val Asn Asn His Glu Glu Gln Arg Asn Asp Val Val Ala Thr Pro Phe
375 380 385att tcc aga tagatcacgg atcggctgct tta 1299Ile Ser Arg390<210>348<211>392<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>348Met Ser Glu Asn Asn Pro Thr Thr Leu His Trp Phe Leu Pro Thr Tyr1 5 10 15Gly Asp Ser Arg Gly Ile Thr Ala Gly Gly His Gly Phe Gly Phe His
20 25 30Ser Gly Ser Arg Thr Ala Asp Leu Asp Tyr Leu Ser Gln Ile Ala Leu
35 40 45Ala Ala Glu Arg Asn Gly Phe Glu Ser Val Leu Thr Pro Thr Gly Leu
50 55 60Trp Cys Glu Asp Ala Trp Ile Thr Thr Ala Ala Leu Leu Ser Arg Thr65 70 75 80Ser Lys Leu Lys Phe Leu Val Ala Ile Arg Pro Gly Gln Val Ser Pro
85 90 95Thr Ile Ile Ala Gln Gln Gly Ala Ala Phe Gln Lys Phe Ser Asn Asn
100 105 110Arg Leu Leu Ile Asn Val Val Val Gly Gly Glu Asp His Glu Gln Arg
115 120 125Ala Phe Ala Asp Tyr Ser Ser Lys Glu Glu Arg Tyr His Lys Ala Asp
130 135 140Glu Thr Leu Glu Ile Ile Asp His Leu Trp Asn Ser Ala Glu Pro Leu145 150 155 160Asn Phe Gln Gly Glu Phe Leu Ser Val Glu Asn Ala Val Leu Lys Glu
165 170 175Gln Pro Glu Val Ser Pro Pro Ile Tyr Phe Gly Gly Ser Ser Gln Leu
180 185 190Gly Ile Glu Ile Ala Ala Gln His Ser Asp Val Tyr Leu Thr Trp Gly
195 200 205Glu Pro Ala Glu Lys Val Glu Glu Lys Leu Ala Arg Val Arg Ala Glu
210 215 220Ala Asp Lys Arg Asn Arg Glu Leu Asp Tyr Gly Ile Arg Leu His Val225 230 235 240Ile Ala Arg Pro Thr Glu Asp Glu Ala Trp Ser Val Ala Gln Asn Leu
245 250 255Leu Asp Gln Leu Asp Gln Glu Glu Val Ala Arg Ile Gln Glu Gly Leu
260 265 270Ala Arg Ser Gln Ser Glu Gly Gln Arg Arg Met Thr Glu Leu His Gly
275 280 285Gln Gly Ala Ala Phe Thr Ala Gly Ala Asp Ala Arg Ser Leu Glu Ile
290 295 300Ala Pro Asn Leu Trp Ala Gly Val Gly Leu Val Arg Gly Gly Ala Gly305 310 315 320Thr Ala Leu Val Gly Ser Tyr Glu Gln Val Ala Gln Ala Ile Leu Arg
325 330 335Tyr Arg Asp Ile Gly Leu Ser His Phe Ile Phe Ser Gly Tyr Pro His
340 345 350Leu Glu Glu Thr Tyr His Val Gly Glu Gly Val Val Pro Glu Leu Leu
355 360 365Lys Leu Gly Val Pro Val Asn Asn His Glu Glu Gln Arg Asn Asp Val
370 375 380Val Ala Thr Pro Phe Ile Ser Arg385 390<210>349<211>681<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(673)<223>RXA01192<400>349ccacggtgaa cacctgcagg tggagcaagc tagccttgcg catccgccag agattattcc 60ggagattctt tttggtggat cgtcgccagc tgcaggtgag gtg gct gca cgt tat 115
Val Ala Ala Arg Tyr
1 5gcg gac acc tat ctc acg tgg ggt gaa act ccc gat cag gtg gcg cag 163Ala Asp Thr Tyr Leu Thr Trp Gly Glu Thr Pro Asp Gln Val Ala Gln
10 15 20aaa atc aac tgg atc aac gag cta gca gca cag cgc ggc cgg gaa ctg 211Lys Ile Asn Trp Ile Asn Glu Leu Ala Ala Gln Arg Gly Arg Glu Leu
25 30 35cgc cat gga atc cgc ttc cat gtg atc acc cgc gat acg tct gaa gaa 259Arg His Gly Ile Arg Phe His Val Ile Thr Arg Asp Thr Ser Glu Glu
40 45 50gca tgg gtg gtg gca gag aag ttg att agc ggg gtc act cca gaa cag 307Ala Trp Val Val Ala Glu Lys Leu Ile Ser Gly Val Thr Pro Glu Gln
55 60 65gtc gct aag gct caa gcc ggg ttt gca acg tct aag tcg gag ggg cag 355Val Ala Lys Ala Gln Ala Gly Phe Ala Thr Ser Lys Ser Glu Gly Gln70 75 80 85cgc cgg atg gct gag ctg cac agc aag ggt cgt gcc ttt act agt ggc 403Arg Arg Met Ala Glu Leu His Ser Lys Gly Arg Ala Phe Thr Ser Gly
90 95 100tca act gct cgt gat ctg gag gtg tat ccc aat gtg tgg gca ggc gtc 451Ser Thr Ala Arg Asp Leu Glu Val Tyr Pro Asn Val Trp Ala Gly Val
105 110 115ggt ttg ctt cgc gga ggt gca gga aca gcc ctt gtg ggc tcg cat gaa 499Gly Leu Leu Arg Gly Gly Ala Gly Thr Ala Leu Val Gly Ser His Glu
120 125 130gag gtc gcc gat cgc atc gaa gaa tac gca gca ctc ggc ttg gat cag 547Glu Val Ala Asp Arg Ile Glu Glu Tyr Ala Ala Leu Gly Leu Asp Gln
135 140 145ttt gta ctg tcg ggt tat cca aac ttg gag gag gcc ttc cac ttc ggt 595Phe Val Leu Ser Gly Tyr Pro Asn Leu Glu Glu Ala Phe His Phe Gly150 155 160 165gag ggt gtg att ccg aaa ctg ctg cgc cgc ggt gtg gat atc aaa aat 643Glu Gly Val Ile Pro Lys Leu Leu Arg Arg Gly Val Asp Ile Lys Asn
170 175 180caa gaa tca cga gtt ttg gaa cct gtt ggg taaacggg 681Gln Glu Ser Arg Val Leu Glu Pro Val Gly
185 190<210>350<211>191<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>350Val Ala Ala Arg Tyr Ala Asp Thr Tyr Leu Thr Trp Gly Glu Thr Pro1 5 10 15Asp Gln Val Ala Gln Lys Ile Asn Trp Ile Asn Glu Leu Ala Ala Gln
20 25 30Arg Gly Arg Glu Leu Arg His Gly Ile Arg Phe His Val Ile Thr Arg
35 40 45Asp Thr Ser Glu Glu Ala Trp Val Val Ala Glu Lys Leu Ile Ser Gly
50 55 60Val Thr Pro Glu Gln Val Ala Lys Ala Gln Ala Gly Phe Ala Thr Ser65 70 75 80Lys Ser Glu Gly Gln Arg Arg Met Ala Glu Leu His Ser Lys Gly Arg
85 90 95Ala Phe Thr Ser Gly Ser Thr Ala Arg Asp Leu Glu Val Tyr Pro Asn
100 105 110Val Trp Ala Gly Val Gly Leu Leu Arg Gly Gly Ala Gly Thr Ala Leu
115 120 125Val Gly Ser His Glu Glu Val Ala Asp Arg Ile Glu Glu Tyr Ala Ala
130 135 140Leu Gly Leu Asp Gln Phe Val Leu Ser Gly Tyr Pro Asn Leu Glu Glu145 150 155 160Ala Phe His Phe Gly Glu Gly Val Ile Pro Lys Leu Leu Arg Arg Gly
165 170 175Val Asp Ile Lys Asn Gln Glu Ser Arg Val Leu Glu Pro Val Gly
180 185 190<210>351<211>918<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(895)<223>RXA00715<400>351gtggtgttaa gcactaagat ggcaggttat gacttctctt aaagtaactt cgtccgcaga 60tgcaaccaat aacaatgatg cccattttcc tgaaggtcca gtg gta acc gtt gac 115
Val Val Thr Val Asp
1 5tgg ttg tca cac aac ctt gac cgg gat gat gtc atc gtg ttg tgt gcc 163Trp Leu Ser His Asn Leu Asp Arg Asp Asp Val Ile Val Leu Cys Ala
10 15 20aca atg gag gat gat gaa att gca cgt caa gcg gga att ccg ggg gca 211Thr Met Glu Asp Asp Glu Ile Ala Arg Gln Ala Gly Ile Pro Gly Ala
25 30 35ttt ctc gct gac ttg gaa gga gat ttc tca gat cca cat tcc gag ctt 259Phe Leu Ala Asp Leu Glu Gly Asp Phe Ser Asp Pro His Ser Glu Leu
40 45 50cca cac acc gcg cca cca aat ttg gtg ggt ttg cta gaa agc tac ggc 307Pro His Thr Ala Pro Pro Asn Leu Val Gly Leu Leu Glu Ser Tyr Gly
55 60 65att agc acc gat tcc acg gtg gtt gtt tat gat ctg cac ggc ctc atg 355Ile Ser Thr Asp Ser Thr Val Val Val Tyr Asp Leu His Gly Leu Met70 75 80 85gtt gca ccg cgg gtg tgg tgg ctt ctc cgt gtt gct gga tta agc agc 403Val Ala Pro Arg Val Trp Trp Leu Leu Arg Val Ala Gly Leu Ser Ser
90 95 100att ggc gtg ctt gat ggc gga ttg cca gcc tgg gtt gat gct ggc ctt 451Ile Gly Val Leu Asp Gly Gly Leu Pro Ala Trp Val Asp Ala Gly Leu
105 110 115cca acg gaa ccg ctg tcg cta cct aca agt ggt gga agg atc agc gca 499Pro Thr Glu Pro Leu Ser Leu Pro Thr Ser Gly Gly Arg Ile Ser Ala
120 125 130gaa cca cag cca gat tta ctc gtt ggt gcc tcc ggc gtt gaa cgg gcg 547Glu Pro Gln Pro Asp Leu Leu Val Gly Ala Ser Gly Val Glu Arg Ala
135 140 145atc gcg cgc tca agc aag gca gtg att gat gct cgt aat gcg agc cga 595Ile Ala Arg Ser Ser Lys Ala Val Ile Asp Ala Arg Asn Ala Ser Arg150 155 160 165ttc gct ggc gtt gaa gaa gag ccc cgt cca ggc ctt cga aaa ggg tcg 643Phe Ala Gly Val Glu Glu Glu Pro Arg Pro Gly Leu Arg Lys Gly Ser
170 175 180atc cct gga agc gtc aac att ccc ttc act gac att tct gat gag cat 691Ile Pro Gly Ser Val Asn Ile Pro Phe Thr Asp Ile Ser Asp Glu His
185 190 195ggt ttt gtc cgg cca gca gaa gaa ctg aag gaa ttg atc ttc agc cgc 739Gly Phe Val Arg Pro Ala Glu Glu Leu Lys Glu Leu Ile Phe Ser Arg
200 205 210aca aat gga gcg cag tcg ttg gtc ttt agc tgt ggc tcc gga gtc acg 787Thr Asn Gly Ala Gln Ser Leu Val Phe Ser Cys Gly Ser Gly Val Thr
215 220 225gca tgt gtt gat gcc tac gct gca gtt atc gca ggt tat gac gac gtt 835Ala Cys Val Asp Ala Tyr Ala Ala Val Ile Ala Gly Tyr Asp Asp Val230 235 240 245gta gtg tat gaa ggc tct tgg gcg gag tgg ggc aac ccg gca aac caa 883Val Val Tyr Glu Gly Ser Trp Ala Glu Trp Gly Asn Pro Ala Asn Gln
250 255 260aag ccg att gct taacgcccgc tatgataacc act 918Lys Pro Ile Ala
265<210>352<211>265<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>352Val Val Thr Val Asp Trp Leu Ser His Asn Leu Asp Arg Asp Asp Val1 5 10 15Ile Val Leu Cys Ala Thr Met Glu Asp Asp Glu Ile Ala Arg Gln Ala
20 25 30Gly Ile Pro Gly Ala Phe Leu Ala Asp Leu Glu Gly Asp Phe Ser Asp
35 40 45Pro His Ser Glu Leu Pro His Thr Ala Pro Pro Asn Leu Val Gly Leu
50 55 60Leu Glu Ser Tyr Gly Ile Ser Thr Asp Ser Thr Val Val Val Tyr Asp65 70 75 80Leu His Gly Leu Met Val Ala Pro Arg Val Trp Trp Leu Leu Arg Val
85 90 95Ala Gly Leu Ser Ser Ile Gly Val Leu Asp Gly Gly Leu Pro Ala Trp
100 105 110Val Asp Ala Gly Leu Pro Thr Glu Pro Leu Ser Leu Pro Thr Ser Gly
115 120 125Gly Arg Ile Ser Ala Glu Pro Gln Pro Asp Leu Leu Val Gly Ala Ser
130 135 140Gly Val Glu Arg Ala Ile Ala Arg Ser Ser Lys Ala Val Ile Asp Ala145 150 155 160Arg Asn Ala Ser Arg Phe Ala Gly Val Glu Glu Glu Pro Arg Pro Gly
165 170 175Leu Arg Lys Gly Ser Ile Pro Gly Ser Val Asn Ile Pro Phe Thr Asp
180 185 190Ile Ser Asp Glu His Gly Phe Val Arg Pro Ala Glu Glu Leu Lys Glu
195 200 205Leu Ile Phe Ser Arg Thr Asn Gly Ala Gln Ser Leu Val Phe Ser Cys
210 215 220Gly Ser Gly Val Thr Ala Cys Val Asp Ala Tyr Ala Ala Val Ile Ala225 230 235 240Gly Tyr Asp Asp Val Val Val Tyr Glu Gly Ser Trp Ala Glu Trp Gly
245 250 255Asn Pro Ala Asn Gln Lys Pro Ile Ala
260 265<210>353<211>945<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(922)<223>RXA01664<400>353cgggttacca aagtgaatgg taggggaagt ttccgtgtct tataccggtt aggttttgcc 60cgcgctgcgc ttggtcacat taacgcctag gctcggggct atg acc gtg ttg att 115
Met Thr Val Leu Ile
1 5tct ccg tcc acc ctt gct gaa tca atc cac gct ggt aag aaa caa act 163Ser Pro Ser Thr Leu Ala Glu Ser Ile His Ala Gly Lys Lys Gln Thr
10 15 20gtt ctc gct gct ttc tgg gct cca att gaa gga gca ggc cgc aca gtt 211Val Leu Ala Ala Phe Trp Ala Pro Ile Glu Gly Ala Gly Arg Thr Val
25 30 35ttc tgc tct gag cac atc cca act tcc att ttc tgc gac cct gcc ctt 259Phe Cys Ser Glu His Ile Pro Thr Ser Ile Phe Cys Asp Pro Ala Leu
40 45 50gag ctt tcc gga gtt cct tcc tct gaa gat ggc cgc aac cca ctg cca 307Glu Leu Ser Gly Val Pro Ser Ser Glu Asp Gly Arg Asn Pro Leu Pro
55 60 65ccg ctg aat gtg ttg gca cgt tct ttc agg acc tgg ggt ttg aat acc 355Pro Leu Asn Val Leu Ala Arg Ser Phe Arg Thr Trp Gly Leu Asn Thr70 75 80 85gat cgt gaa atc gtg ttt tac gat cag gga cgt ggc ctt ttt gct gca 403Asp Arg Glu Ile Val Phe Tyr Asp Gln Gly Arg Gly Leu Phe Ala Ala
90 95 100cgc gcc tgg tgg atc ctc cga tgg gcg ggc atg ccc aac gtt cgc atc 451Arg Ala Trp Trp Ile Leu Arg Trp Ala Gly Met Pro Asn Val Arg Ile
105 110 115ctt gac ggt ggt ttc cag aag tgg gaa gac cat gag ctg gga cac gct 499Leu Asp Gly Gly Phe Gln Lys Trp Glu Asp His Glu Leu Gly His Ala
120 125 130ggc ggg cct gga aac ttc ccg cac ttt tgc aat gtg cgt ccc aac cca 547Gly Gly Pro Gly Asn Phe Pro His Phe Cys Asn Val Arg Pro Asn Pro
135 140 145ggt cag ctg tcg gta gcg acc atc gaa gat gtc aag gca cat cag ggc 595Gly Gln Leu Ser Val Ala Thr Ile Glu Asp Val Lys Ala His Gln Glyl50 155 160 165att ttg att gat tct cgc gat gaa caa cga ttt gcg ggt cgc agt gaa 643Ile Leu Ile Asp Ser Arg Asp Glu Gln Arg Phe Ala Gly Arg Ser Glu
170 175 180aag ctc gat ctg aaa gcc gga cac att cca ggc gct atc aac atc aac 691Lys Leu Asp Leu Lys Ala Gly His Ile Pro Gly Ala Ile Asn Ile Asn
185 190 195gct aaa tct ttg ctg gaa gat gat ttc acc ttc aaa tca cca gaa gaa 739Ala Lys Ser Leu Leu Glu Asp Asp Phe Thr Phe Lys Ser Pro Glu Glu
200 205 210atc cgc cag att ttt gcg gac aag ggg gta acc agc gga gag aac gtc 787Ile Arg Gln Ile Phe Ala Asp Lys Gly Val Thr Ser Gly Glu Asn Val
215 220 225atc gtt tat tcc ggt tcc ggt aac cac tcg tcc cag ttg ctg gct ggc 835Ile Val Tyr Ser Gly Ser Gly Asn His Ser Ser Gln Leu Leu Ala Gly230 235 240 245atg gag cac gcg ggg cta acc ggt gcg agc cat tat ttt gct ggt tgg 883Met Glu His Ala Gly Leu Thr Gly Ala Ser His Tyr Phe Ala Gly Trp
250 255 260tca cag tgg agc gct aac ccc gag aat cct atc gag gcc taaaatcgtg 932Ser Gln Trp Ser Ala Asn Pro Glu Asn Pro Ile Glu Ala
265 270gcttgagtac gca 945<210>354<211>274<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>354Met Thr Val Leu Ile Ser Pro Ser Thr Leu Ala Glu Ser Ile His Ala1 5 10 15Gly Lys Lys Gln Thr Val Leu Ala Ala Phe Trp Ala Pro Ile Glu Gly
20 25 30Ala Gly Arg Thr Val Phe Cys Ser Glu His Ile Pro Thr Ser Ile Phe
35 40 45Cys Asp Pro Ala Leu Glu Leu Ser Gly Val Pro Ser Ser Glu Asp Gly
50 55 60Arg Asn Pro Leu Pro Pro Leu Asn Val Leu Ala Arg Ser Phe Arg Thr65 70 75 80Trp Gly Leu Asn Thr Asp Arg Glu Ile Val Phe Tyr Asp Gln Gly Arg
85 90 95Gly Leu Phe Ala Ala Arg Ala Trp Trp Ile Leu Arg Trp Ala Gly Met
100 105 110Pro Asn Val Arg Ile Leu Asp Gly Gly Phe Gln Lys Trp Glu Asp His
115 120 125Glu Leu Gly His Ala Gly Gly Pro Gly Asn Phe Pro His Phe Cys Asn
130 135 140Val Arg Pro Asn Pro Gly Gln Leu Ser Val Ala Thr Ile Glu Asp Val145 150 155 160Lys Ala His Gln Gly Ile Leu Ile Asp Ser Arg Asp Glu Gln Arg Phe
165 170 175Ala Gly Arg Ser Glu Lys Leu Asp Leu Lys Ala Gly His Ile Pro Gly
180 185 190Ala Ile Asn Ile Asn Ala Lys Ser Leu Leu Glu Asp Asp Phe Thr Phe
195 200 205Lys Ser Pro Glu Glu Ile Arg Gln Ile Phe Ala Asp Lys Gly Val Thr
210 215 220Ser Gly Glu Asn Val Ile Val Tyr Ser Gly Ser Gly Asn His Ser Ser225 230 235 240Gln Leu Leu Ala Gly Met Glu His Ala Gly Leu Thr Gly Ala Ser His
245 250 255Tyr Phe Ala Gly Trp Ser Gln Trp Ser Ala Asn Pro Glu Asn Pro Ile
260 265 270Glu Ala<210>355<211>746<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(723)<223>RXN02334<400>355gtc aaa gac ctc aac gat ccc ctc acc cgc gat ttc att gac ggt gaa 48Val Lys Asp Leu Asn Asp Pro Leu Thr Arg Asp Phe Ile Asp Gly Glu1 5 10 15gct ttc gct gag ctg atg aac cgc aag ggc atc gct cgc gat gac acc 96Ala Phe Ala Glu Leu Met Asn Arg Lys Gly Ile Ala Arg Asp Asp Thr
20 25 30gtt gtt gtc tac ggt gac aag tcc aac tgg tgg gct gcg ttc acc ctg 144Val Val Val Tyr Gly Asp Lys Ser Asn Trp Trp Ala Ala Phe Thr Leu
35 40 45tgg gtc ttc gaa ctg ttc ggc cac tcc gat gtc cgc ctg ctc aac ggc 192Trp Val Phe Glu Leu Phe Gly His Ser Asp Val Arg Leu Leu Asn Gly
50 55 60ggc cgc gac gcg tgg atg gct gaa gag cgc gac acc tcc tac gtg gtt 240Gly Arg Asp Ala Trp Met Ala Glu Glu Arg Asp Thr Ser Tyr Val Val65 70 75 80ccg gag tac ccc tcc gcc aac tac ccc gtc gtg gag cgt gtc gac gaa 288Pro Glu Tyr Pro Ser Ala Asn Tyr Pro Val Val Glu Arg Val Asp Glu
85 90 95aac cag cgc gcg ttc gtg gct gag gtg ctc ggt tcg ctc acg caa tcc 336Asn Gln Arg Ala Phe Val Ala Glu Val Leu Gly Ser Leu Thr Gln Ser
100 105 110ggt ggc atg acg ctt gtc gac gtc agg acc cct tcg gag ttc tcc gga 384Gly Gly Met Thr Leu Val Asp Val Arg Thr Pro Ser Glu Phe Ser Gly
115 120 125ttg gat gag cac ggc aac cca acc tca aac acc ggc gtg ctt cgt ggt 432Leu Asp Glu His Gly Asn Pro Thr Ser Asn Thr Gly Val Leu Arg Gly
130 135 140gga cac atc cca ggc gcg atc aac ctg gat tgg tcg gac gct gtt ctt 480Gly His Ile Pro Gly Ala Ile Asn Leu Asp Trp Ser Asp Ala Val Leu145 150 155 160ccc aac gga aac ttc cgc acc cgt gca gag ttg gac aag ctc tac gcc 528Pro Asn Gly Asn Phe Arg Thr Arg Ala Glu Leu Asp Lys Leu Tyr Ala
165 170 175gat ctc aac cca gct gac gat acc gtt gtc tac tgc cag gtt ggc gac 576Asp Leu Asn Pro Ala Asp Asp Thr Val Val Tyr Cys Gln Val Gly Asp
180 185 190cgc gcg gcc cac acc tgg ttc gtg ctg aag tat ctg ctc ggt ttc aac 624Arg Ala Ala His Thr Trp Phe Val Leu Lys Tyr Leu Leu Gly Phe Asn
195 200 205aac gtc cga aac tat gac gga tcg tgg gca gaa tgg ggc aat atg gtt 672Asn Val Arg Asn Tyr Asp Gly Ser Trp Ala Glu Trp Gly Asn Met Val
210 215 220cgc atg ccg atc gaa act ggc gaa aac acc aaa aat aac gtt tcg gtg 720Arg Met Pro Ile Glu Thr Gly Glu Asn Thr Lys Asn Asn Val Ser Val225 230 235 240tca tagaataggc gtatcccctt ttt 746Ser<210>356<211>241<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>356Val Lys Asp Leu Asn Asp Pro Leu Thr Arg Asp Phe Ile Asp Gly Glu1 5 10 15Ala Phe Ala Glu Leu Met Asn Arg Lys Gly Ile Ala Arg Asp Asp Thr
20 25 30Val Val Val Tyr Gly Asp Lys Ser Asn Trp Trp Ala Ala Phe Thr Leu
35 40 45Trp Val Phe Glu Leu Phe Gly His Ser Asp Val Arg Leu Leu Asn Gly
50 55 60Gly Arg Asp Ala Trp Met Ala Glu Glu Arg Asp Thr Ser Tyr Val Val65 70 75 80Pro Glu Tyr Pro Ser Ala Asn Tyr Pro Val Val Glu Arg Val Asp Glu
85 90 95Asn Gln Arg Ala Phe Val Ala Glu Val Leu Gly Ser Leu Thr Gln Ser
100 105 110Gly Gly Met Thr Leu Val Asp Val Arg Thr Pro Ser Glu Phe Ser Gly
115 120 125Leu Asp Glu Hi s Gly Asn Pro Thr Ser Asn Thr Gly Val Leu Arg Gly
130 135 140Gly His Ile Pro Gly Ala Ile Asn Leu Asp Trp Ser Asp Ala Val Leu145 150 155 160Pro Asn Gly Asn Phe Arg Thr Arg Ala Glu Leu Asp Lys Leu Tyr Ala
165 170 175Asp Leu Asn Pro Ala Asp Asp Thr Val Val Tyr Cys Gln Val Gly Asp
180 185 190Arg Ala Ala His Thr Trp Phe Val Leu Lys Tyr Leu Leu Gly Phe Asn
195 200 205Asn Val Arg Asn Tyr Asp Gly Ser Trp Ala Glu Trp Gly Asn Met Val
210 215 220Arg Met Pro Ile Glu Thr Gly Glu Asn Thr Lys Asn Asn Val Ser Val225 230 235 240Ser<210>357<211>377<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(354)<223>FRXA02334<400>357gga gtt ctc ccg gat tgg atg agc acg gca acc caa ccc tca aac acc 48Gly Val Leu Pro Asp Trp Met Ser Thr Ala Thr Gln Pro Ser Asn Thr1 5 10 15ggc gtg ctt cgt ggt gaa cac atc cca ggc gcg atc aac ctg gat tgg 96Gly Val Leu Arg Gly Glu His Ile Pro Gly Ala Ile Asn Leu Asp Trp
20 25 30tcg gac gct gtt ctt ccc aac gga aac ttc cgc acc cgt gca gag ttg 144Ser Asp Ala Val Leu Pro Asn Gly Asn Phe Arg Thr Arg Ala Glu Leu
35 40 45gac aag ctc tac gcc gat ctc aac cca gct gac gat acc gtt gtc tac 192Asp Lys Leu Tyr Ala Asp Leu Asn Pro Ala Asp Asp Thr Val Val Tyr
50 55 60tgc cag gtt ggc gac cgc gcg gcc cac acc tgg ttc gtg ctg aag tat 240Cys Gln Val Gly Asp Arg Ala Ala His Thr Trp Phe Val Leu Lys Tyr65 70 75 80ctg ctc ggt ttc aac aac gtc cga aac tat gac gga tcg tgg gca gaa 288Leu Leu Gly Phe Asn Asn Val Arg Asn Tyr Asp Gly Ser Trp Ala Glu
85 90 95tgg ggc aat atg gtt cgc atg ccg atc gaa act ggc gaa aac acc aaa 336Trp Gly Asn Met Val Arg Met Pro Ile Glu Thr Gly Glu Asn Thr Lys
100 105 110aat aac gtt tcg gtg tca tagaataggc gtatcccctt ttt 377Asn Asn Val Ser Val Ser
115<210>358<211>118<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>358Gly Val Leu Pro Asp Trp Met Ser Thr Ala Thr Gln Pro Ser Asn Thr1 5 10 15Gly Val Leu Arg Gly Glu His Ile Pro Gly Ala Ile Asn Leu Asp Trp
20 25 30Ser Asp Ala Val Leu Pro Asn Gly Asn Phe Arg Thr Arg Ala Glu Leu
35 40 45Asp Lys Leu Tyr Ala Asp Leu Asn Pro Ala Asp Asp Thr Val Val Tyr
50 55 60Cys Gln Val Gly Asp Arg Ala Ala His Thr Trp Phe Val Leu Lys Tyr65 70 75 80Leu Leu Gly Phe Asn Asn Val Arg Asn Tyr Asp Gly Ser Trp Ala Glu
85 90 95Trp Gly Asn Met Val Arg Met Pro Ile Glu Thr Gly Glu Asn Thr Lys
100 105 110Asn Asn Val Ser Val Ser
115<210>359<211>3945<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(3922)<223>RXN01499<400>359gcagcaatta tctccaccga agaggactaa atataacgtg gcattgagca gtgttccagc 60acagttcctg agatccgccc aggcgccccc gaagcgtact ttg tgg gac gtc tta 115
Leu Trp Asp Val Leu
1 5gaa tcc gtc gcc tct act tat cct gag gca gca gct att gac gat ggc 163Glu Ser Val Ala Ser Thr Tyr Pro Glu Ala Ala Ala Ile Asp Asp Gly
10 15 20cag gtg ttg acc tac gca gag ttg atg gaa gaa gtc acc gcg ttg gct 211Gln Val Leu Thr Tyr Ala Glu Leu Met Glu Glu Val Thr Ala Leu Ala
25 30 35gat tcc att cat gca cag ggc att cgc cgt ggt gat cgc atc ggt att 259Asp Ser Ile His Ala Gln Gly Ile Arg Arg Gly Asp Arg Ile Gly Ile
40 45 50cgc atg ccg tct ggt acg cgt gac ctt tac atc gct att ttg gcc act 307Arg Met Pro Ser Gly Thr Arg Asp Leu Tyr Ile Ala Ile Leu Ala Thr
55 60 65ctc gct gct ggt gct gct tac gtg cca gtt gat gca gat gat cct gaa 355Leu Ala Ala Gly Ala Ala Tyr Val Pro Val Asp Ala Asp Asp Pro Glu70 75 80 85gag cgc gcc gag atg gtg ttt ggt gaa gca aat att aat gcg ctt ttc 403Glu Arg Ala Glu Met Val Phe Gly Glu Ala Asn Ile Asn Ala Leu Phe
90 95 100gac gcc acc ggc ttc cat atg ctt cgc ccg acc gcg ggc ggc gat acc 451Asp Ala Thr Gly Phe His Met Leu Arg Pro Thr Ala Gly Gly Asp Thr
105 110 115cgt aga cca cgc ttg gat gat acg gcg tgg att atc ttt act tcc ggt 499Arg Arg Pro Arg Leu Asp Asp Thr Ala Trp Ile Ile Phe Thr Ser Gly
120 125 130tcc acc ggc aag cct aag ggt gtg gct gtg tcc cac cgt tca gct gcg 547Ser Thr Gly Lys Pro Lys Gly Val Ala Val Ser His Arg Ser Ala Ala
135 140 145gct ttc gtg gat gcc gaa gca caa atg ttc ctt gtc gat cac cct tcc 595Ala Phe Val Asp Ala Glu Ala Gln Met Phe Leu Val Asp His Pro Ser150 155 160 165ggc ccc ctt ggc cca gaa gac cga gtc ctt gcg gga ttg tct gta gcc 643Gly Pro Leu Gly Pro Glu Asp Arg Val Leu Ala Gly Leu Ser Val Ala
170 175 180ttt gac gca tct tgt gag gaa atg tgg ttg gcc tgg ggc cac ggc gcc 691Phe Asp Ala Ser Cys Glu Glu Met Trp Leu Ala Trp Gly His Gly Ala
185 190 195tgc ttg gtg cca gca cca cgc tcc cta gtc cgt tcc ggt atg gac ttg 739Cys Leu Val Pro Ala Pro Arg Ser Leu Val Arg Ser Gly Met Asp Leu
200 205 210ggc cca tgg ctg att cgc cgc gac atc agt gtc gtc tcc acc gtc cca 787Gly Pro Trp Leu Ile Arg Arg Asp Ile Ser Val Val Ser Thr Val Pro
215 220 225act ctg gct ggt ctg tgg cca gca gaa gca ttg tca cag gtc cgc ttg 835Thr Leu Ala Gly Leu Trp Pro Ala Glu Ala Leu Ser Gln Val Arg Leu230 235 240 245ctc atc gtc ggc ggc gag gct tgc tcg cag gag ctc gtt gaa cgc tta 883Leu Ile Val Gly Gly Glu Ala Cys Ser Gln Glu Leu Val Glu Arg Leu
250 255 260tcg acg cct gac cgc gag gtg tgg aac act tac ggc ccc acc gaa gca 931Ser Thr Pro Asp Arg Glu Val Trp Asn Thr Tyr Gly Pro Thr Glu Ala
265 270 275acg gtg gtt gcc tgt ggc act caa ctc tat gct ggt cag cca gtg ggc 979Thr Val Val Ala Cys Gly Thr Gln Leu Tyr Ala Gly Gln Pro Val Gly
280 285 290att ggt ttg cca ctt gct ggt tgg gat ctt gtt gtt gtc gac gat gcc 1027Ile Gly Leu Pro Leu Ala Gly Trp Asp Leu Val Val Val Asp Asp Ala
295 300 305ggc gaa cct gtc gga atc ggc gag gtc ggc gaa ttg gtc atc ggt ggt 1075Gly Glu Pro Val Gly Ile Gly Glu Val Gly Glu Leu Val Ile Gly Gly310 315 320 325gtg ggt ctt gca cgc tac ctt gat cca gaa aaa gac cgc gag aag tat 1123Val Gly Leu Ala Arg Tyr Leu Asp Pro Glu Lys Asp Arg Glu Lys Tyr
330 335 340gcg cca ctg aag tct gtt ggt tgg acc cgc gct tat cgt tcc ggt gac 1171Ala Pro Leu Lys Ser Val Gly Trp Thr Arg Ala Tyr Arg Ser Gly Asp
345 350 355cac gtt cgt ctg gaa gaa gat ggc ctc tac ttt gtg ggc cgc gtt gat 1219His Val Arg Leu Glu Glu Asp Gly Leu Tyr Phe Val Gly Arg Val Asp
360 365 370gat cag gtg aaa atc ggc ggt cga cgc atc gag ctc ggt gaa gtt gat 1267Asp Gln Val Lys Ile Gly Gly Arg Arg Ile Glu Leu Gly Glu Val Asp
375 380 385gcc aat gtg gca gcg ctt tcc aac gtt cgt tcc tcc gca gtg gtt gtt l315Ala Asn Val Ala Ala Leu Ser Asn Val Arg Ser Ser Ala Val Val Val390 395 400 405cag acc act ggt gcg gat caa aaa gtt ctg gtt gca tac gtt tct ttg 1363Gln Thr Thr Gly Ala Asp Gln Lys Val Leu Val Ala Tyr Val Ser Leu
410 415 420gaa gat gct gca gct gga ttt gat cac aac gtc gcg act gcc cga ctc 1411Glu Asp Ala Ala Ala Gly Phe Asp His Asn Val Ala Thr Ala Arg Leu
425 430 435acc gaa acc atg cct gct gct ttg gtt ccg cgc att cac gtg atg gat 1459Thr Glu Thr Met Pro Ala Ala Leu Val Pro Arg Ile His Val Met Asp
440 445 450gat ctg cct gtc acc acc tcc ggc aag gtt gat aag aag tct ttg ccg 1507Asp Leu Pro Val Thr Thr Ser Gly Lys Val Asp Lys Lys Ser Leu Pro
455 460 465tgg cct ctt cct ggc acc gtg gtg gaa gct aat gac ctc agc gca acg 1555Trp Pro Leu Pro Gly Thr Val Val Glu Ala Asn Asp Leu Ser Ala Thr470 475 480 485gaa gcg tgg att gct cag gaa tgg gtc gat atc ctc ggc act tct gtg 1603Glu Ala Trp Ile Ala Gln Glu Trp Val Asp Ile Leu Gly Thr Ser Val
490 495 500agc agc aaa gac gcc gac ttc ttc tcc ctt ggc ggt acc tct ctc gcg 1651Ser Ser Lys Asp Ala Asp Phe Phe Ser Leu Gly Gly Thr Ser Leu Ala
505 510 515gct gcg act ttg gtt ggc cgg gta cgc gca aag gtt ccc acc gct gcg 1699Ala Ala Thr Leu Val Gly Arg Val Arg Ala Lys Val Pro Thr Ala Ala
520 525 530gtg cgt gat ctt tac gat cac cct cgc ttg gag aaa ttc gcc gag cgt 1747Val Arg Asp Leu Tyr Asp His Pro Arg Leu Glu Lys Phe Ala Glu Arg
535 540 545gtc gag gct atc gcc gcc gac act ggc att tct ttg gag gcg cca aac 1795Val Glu Ala Ile Ala Ala Asp Thr Gly Ile Ser Leu Glu Ala Pro Asn550 555 560 565cag gtg gag gag cgc gtc gtc aag cct gtt tct ttt ggc act cgt gtg 1843Gln Val Glu Glu Arg Val Val Lys Pro Val Ser Phe Gly Thr Arg Val
570 575 580atg cag acc ctc atc cag att ccg atc atg acg ctg caa gca gca cag 1891Met Gln Thr Leu Ile Gln Ile Pro Ile Met Thr Leu Gln Ala Ala Gln
585 590 595tgg att gca tgg ttg ctg ttg ggc aac aac atc atg gca gcg ctt gat 1939Trp Ile Ala Trp Leu Leu Leu Gly Asn Asn Ile Met Ala Ala Leu Asp
600 605 610ttc gat tgg gct gtt cat gtc tcc tgg tgg ctt gtc atc ggc atg att 1987Phe Asp Trp Ala Val His Val Ser Trp Trp Leu Val Ile Gly Met Ile
615 620 625ttg gtg ttc gct acc ccg att ggt cgc ttg ccg atc ggc ggt tgg ggc 2035Leu Val Phe Ala Thr Pro Ile Gly Arg Leu Pro Ile Gly Gly Trp Gly630 635 640 645gcc cgc atc atc acc cgt ggc ata act cct ggc tcc tac cct cgt ggc 2083Ala Arg Ile Ile Thr Arg Gly Ile Thr Pro Gly Ser Tyr Pro Arg Gly
650 655 660ggt tcc act cac ctg cgc att tgg tcc gcc gag cgc ctt gct gat gcc 2131Gly Ser Thr His Leu Arg Ile Trp Ser Ala Glu Arg Leu Ala Asp Ala
665 670 675tct ggc tct cgc aat att tct ggc gca acc tgg gtg aac tac ttc gcg 2179Ser Gly Ser Arg Asn Ile Ser Gly Ala Thr Trp Val Asn Tyr Phe Ala
680 685 690cgt tcc ctg ggt gtg aag atg ggc aag ggc gtg gat ctt cac tcc ctg 2227Arg Ser Leu Gly Val Lys Met Gly Lys Gly Val Asp Leu His Ser Leu
695 700 705cca cca atc act ggc ctt ttg acc ttg ggc aac aat gtt tcc atc gag 2275Pro Pro Ile Thr Gly Leu Leu Thr Leu Gly Asn Asn Val Ser Ile Glu710 715 720 725caa gaa gtt gac ctt cgt ggc tac tgg ctc gac ggc gat atc ctg cgt 2323Gln Glu Val Asp Leu Arg Gly Tyr Trp Leu Asp Gly Asp Ile Leu Arg
730 735 740gta ggc acc att gag gtc cat gac aac gct cgc atc ggc gct cgt tcc 2371Val Gly Thr Ile Glu Val His Asp Asn Ala Arg Ile Gly Ala Arg Ser
745 750 755acc ctg ctt ccc ggc acc gtg gtg ggc acc ggc gct cac ctg ctg cct 2419Thr Leu Leu Pro Gly Thr Val Val Gly Thr Gly Ala His Leu Leu Pro
760 765 770ggt tca aca gtg act ggt gat aag acc atc aag cct ggt tct cgt tgg 2467Gly Ser Thr Val Thr Gly Asp Lys Thr Ile Lys Pro Gly Ser Arg Trp
775 780 785gct ggc tcc cct gca caa aag gtg ggt cgt gca aag cac cgg ttc cca 2515Ala Gly Ser Pro Ala Gln Lys Val Gly Arg Ala Lys His Arg Phe Pro790 795 800 805acc tcc cat cct cca cgc agg tcc cgg tgg gtt ccg gtg ttc ggc gcg 2563Thr Ser His Pro Pro Arg Arg Ser Arg Trp Val Pro Val Phe Gly Ala
810 815 820acc tcc atc gtg ttg tcg ctg ctg cca ctt cag gct ctc gct att ggc 2611Thr Ser Ile Val Leu Ser Leu Leu Pro Leu Gln Ala Leu Ala Ile Gly
825 830 835gct gct atc acc ttg tgg ctg gcc acg att agc ccg ctt cca ctg atc 2659Ala Ala Ile Thr Leu Trp Leu Ala Thr Ile Ser Pro Leu Pro Leu Ile
840 845 850tgg ggt gtg ctg gtt ttt gct acc gtc ggc gcg ttg gct gcg ttc ttt 2707Trp Gly Val Leu Val Phe Ala Thr Val Gly Ala Leu Ala Ala Phe Phe
855 860 865gct tac acc gtg acc atc tgg gtg ctt gtc cgt ttg atc cag atc ggc 2755Ala Tyr Thr Val Thr Ile Trp Val Leu Val Arg Leu Ile Gln Ile Gly870 875 880 885atc aag ggc ggc acc gca cca gtg agg tcc cgt ctt ggt tgg cag gtc 2803Ile Lys Gly Gly Thr Ala Pro Val Arg Ser Arg Leu Gly Trp Gln Val
890 895 900tgg gca gtt caa cgc ctc atg gac gat gcc cgc acc tat ctc ttc ccg 2851Trp Ala Val Gln Arg Leu Met Asp Asp Ala Arg Thr Tyr Leu Phe Pro
905 910 915ctc tac gca tcc caa ctg acc cca ctg tgg ttc cgc agc ttg ggc gcg 2899Leu Tyr Ala Ser Gln Leu Thr Pro Leu Trp Phe Arg Ser Leu Gly Ala
920 925 930aag atc ggc aag gat gtt gag atc tcc acc gcg gtg atg gtt cct aaa 2947Lys Ile Gly Lys Asp Val Glu Ile Ser Thr Ala Val Met Val Pro Lys
935 940 945ctg gct gat atc cgc gaa ggc gca ttc ctg gcc gat gac acc ctc atc 2995Leu Ala Asp Ile Arg Glu Gly Ala Phe Leu Ala Asp Asp Thr Leu Ile950 955 960 965ggt ggc tat gag ctg ggt aat ggt tgg ctg ctc agt ggt gaa acc cgc 3043Gly Gly Tyr Glu Leu Gly Asn Gly Trp Leu Leu Ser Gly Glu Thr Arg
970 975 980gtg ggt aag cgt tcc ttc att ggt aac tct ggc atc gca gga cct gag 3091Val Gly Lys Arg Ser Phe Ile Gly Asn Ser Gly Ile Ala Gly Pro Glu
985 990 995cgc aag ctc gct aag aac tcc ctg gtt gca gtg ctc tcc tcc acc ccg 3139Arg Lys Leu Ala Lys Asn Ser Leu Val Ala Val Leu Ser Ser Thr Pro
1000 1005 1010aag aag gct aag gcc aac tcc aac tgg tgg ggt tcc cct cca gag cgc 3187Lys Lys Ala Lys Ala Asn Ser Asn Trp Trp Gly Ser Pro Pro Glu Arg
1015 1020 1025atg cgt cgt gtc act gtc gaa gtt gat gag ggc gaa gca aag acc tac 3235Met Arg Arg Val Thr Val Glu Val Asp Glu Gly Glu Ala Lys Thr Tyr1030 1035 1040 1045agc cct ggc ttt ggt gtg aag ttt gca cgt ggc gcg gtg gaa acc gca 3283Ser Pro Gly Phe Gly Val Lys Phe Ala Arg Gly Ala Val Glu Thr Ala
1050 1055 1060cgt ctg ctt gct cca ata acc tct ggt gtg ttg gct gcg ctg tca ctg 3331Arg Leu Leu Ala Pro Ile Thr Ser Gly Val Leu Ala Ala Leu Ser Leu
1065 1070 1075ctg ctc atg cag tac ctg ctc act gag ttc aac atg tgg atc acc tgg 3379Leu Leu Met Gln Tyr Leu Leu Thr Glu Phe Asn Met Trp Ile Thr Trp
1080 1085 1090ttg ctt ggc gga ctg atc ctc atg acg gtt ggt gtg ctc gcc atg ggc 3427Leu Leu Gly Gly Leu Ile Leu Met Thr Val Gly Val Leu Ala Met Gly
1095 1100 1105att acg gtt gtg atg aag tgg gtt tgc gtc ggc aag cat aag ccg tct 3475Ile Thr Val Val Met Lys Trp Val Cys Val Gly Lys His Lys Pro Ser1110 1115 1120 1125gag cac cct ctc ttc agc cgc ttt gtg tgg ctg aat gag ctg caa gat 3523Glu His Pro Leu Phe Ser Arg Phe Val Trp Leu Asn Glu Leu Gln Asp
1130 1135 1140gcg ttc gtg gaa tcc gtg gct ggc cca tgg ttc ctc gtg ccc aac ctg 3571Ala Phe Val Glu Ser Val Ala Gly Pro Trp Phe Leu Val Pro Asn Leu
1145 1150 1155ggc acc ggc gcg ctg aac gcc ggc atg agc gcg ctt ggc gca cac atc 3619Gly Thr Gly Ala Leu Asn Ala Gly Met Ser Ala Leu Gly Ala His I1e
1160 1165 1170ggc cgt ggc gca tgg atc gaa tcc tac tgg ctg ccg gaa acc gac ctc 3667Gly Arg Gly Ala Trp Ile Glu Ser Tyr Trp Leu Pro Glu Thr Asp Leu
1175 1180 1185tgc tac atc ggc aag ggc gca acc gtg ggc cct ggc gtg gtc gtg cag 3715Cys Tyr Ile Gly Lys Gly Ala Thr Val Gly Pro Gly Val Val Val Gln1190 1195 1200 1205acc cac ctc ttc cag gac cgc gtg atg agc cta gat acg gtg acc gtc 3763Thr His Leu Phe Gln Asp Arg Val Met Ser Leu Asp Thr Val Thr Val
1210 1215 1220gct gac ggc gcc acc cta gcg gac cac tcc gtt gcc ctt cct gct tcg 3811Ala Asp Gly Ala Thr Leu Ala Asp His Ser Val Ala Leu Pro Ala Ser
1225 1230 1235ctt atc gac gcc tcc gcc acc atc ggc cca ggc tcg ctg gtg atg cgc 3859Leu Ile Asp Ala Ser Ala Thr Ile Gly Pro Gly Ser Leu Val Met Arg
1240 1245 1250ggc gac aag gta cca gcg cat acc cgc tgg caa ggc aac cca att gag 3907Gly Asp Lys Val Pro Ala His Thr Arg Trp Gln Gly Asn Pro Ile Glu
1255 1260 1265ccg tgg agc aac tct taaataacaa caatcagccg gat 3945Pro Trp Ser Asn Ser1270<210>360<211>1274<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>360Leu Trp Asp Val Leu Glu Ser Val Ala Ser Thr Tyr Pro Glu Ala Ala1 5 10 15Ala Ile Asp Asp Gly Gln Val Leu Thr Tyr Ala Glu Leu Met Glu Glu
20 25 30Val Thr Ala Leu Ala Asp Ser Ile His Ala Gln Gly Ile Arg Arg Gly
35 40 45Asp Arg Ile Gly Ile Arg Met Pro Ser Gly Thr Arg Asp Leu Tyr Ile
50 55 60Ala Ile Leu Ala Thr Leu Ala Ala Gly Ala Ala Tyr Val Pro Val Asp65 70 75 80Ala Asp Asp Pro Glu Glu Arg Ala Glu Met Val Phe Gly Glu Ala Asn
85 90 95Ile Asn Ala Leu Phe Asp Ala Thr Gly Phe His Met Leu Arg Pro Thr
100 105 110Ala Gly Gly Asp Thr Arg Arg Pro Arg Leu Asp Asp Thr Ala Trp Ile
115 120 125Ile Phe Thr Ser Gly Ser Thr Gly Lys Pro Lys Gly Val Ala Val Ser
130 135 140His Arg Ser Ala Ala Ala Phe Val Asp Ala Glu Ala Gln Met Phe Leu145 150 155 160Val Asp His Pro Ser Gly Pro Leu Gly Pro Glu Asp Arg Val Leu Ala
165 170 175Gly Leu Ser Val Ala Phe Asp Ala Ser Cys Glu Glu Met Trp Leu Ala
180 185 190Trp Gly His Gly Ala Cys Leu Val Pro Ala Pro Arg Ser Leu Val Arg
195 200 205Ser Gly Met Asp Leu Gly Pro Trp Leu Ile Arg Arg Asp Ile Ser Val
2l0 215 220Val Ser Thr Val Pro Thr Leu Ala Gly Leu Trp Pro Ala Glu Ala Leu225 230 235 240Ser Gln Val Arg Leu Leu Ile Val Gly Gly Glu Ala Cys Ser Gln Glu
245 250 255Leu Val Glu Arg Leu Ser Thr Pro Asp Arg Glu Val Trp Asn Thr Tyr
260 265 270Gly Pro Thr Glu Ala Thr Val Val Ala Cys Gly Thr Gln Leu Tyr Ala
275 280 285Gly Gln Pro Val Gly Ile Gly Leu Pro Leu Ala Gly Trp Asp Leu Val
290 295 300Val Val Asp Asp Ala Gly Glu Pro Val Gly Ile Gly Glu Val Gly Glu305 310 315 320Leu Val Ile Gly Gly Val Gly Leu Ala Arg Tyr Leu Asp Pro Glu Lys
325 330 335Asp Arg Glu Lys Tyr Ala Pro Leu Lys Ser Val Gly Trp Thr Arg Ala
340 345 350Tyr Arg Ser Gly Asp His Val Arg Leu Glu Glu Asp Gly Leu Tyr Phe
355 360 365Val Gly Arg Val Asp Asp Gln Val Lys Ile Gly Gly Arg Arg Ile Glu
370 375 380Leu Gly Glu Val Asp Ala Asn Val Ala Ala Leu Ser Asn Val Arg Ser385 390 395 400Ser Ala Val Val Val Gln Thr Thr Gly Ala Asp Gln Lys Val Leu Val
405 410 415Ala Tyr Val Ser Leu Glu Asp Ala Ala Ala Gly Phe Asp His Asn Val
420 425 430Ala Thr Ala Arg Leu Thr Glu Thr Met Pro Ala Ala Leu Val Pro Arg
435 440 445Ile His Val Met Asp Asp Leu Pro Val Thr Thr Ser Gly Lys Val Asp
450 455 460Lys Lys Ser Leu Pro Trp Pro Leu Pro Gly Thr Val Val Glu Ala Asn465 470 475 480Asp Leu Ser Ala Thr Glu Ala Trp Ile Ala Gln Glu Trp Val Asp Ile
485 490 495Leu Gly Thr Ser Val Ser Ser Lys Asp Ala Asp Phe Phe Ser Leu Gly
500 505 510Gly Thr Ser Leu Ala Ala Ala Thr Leu Val Gly Arg Val Arg Ala Lys
515 520 525Val Pro Thr Ala Ala Val Arg Asp Leu Tyr Asp His Pro Arg Leu Glu
530 535 540Lys Phe Ala Glu Arg Val Glu Ala Ile Ala Ala Asp Thr Gly Ile Ser545 550 555 560Leu Glu Ala Pro Asn Gln Val Glu Glu Arg Val Val Lys Pro Val Ser
565 570 575Phe Gly Thr Arg Val Met Gln Thr Leu Ile Gln Ile Pro Ile Met Thr
580 585 590Leu Gln Ala Ala Gln Trp Ile Ala Trp Leu Leu Leu Gly Asn Asn Ile
595 600 605Met Ala Ala Leu Asp Phe Asp Trp Ala Val His Val Ser Trp Trp Leu
610 615 620Val Ile Gly Met Ile Leu Val Phe Ala Thr Pro Ile Gly Arg Leu Pro625 630 635 640Ile Gly Gly Trp Gly Ala Arg Ile Ile Thr Arg Gly Ile Thr Pro Gly
645 650 655Ser Tyr Pro Arg Gly Gly Ser Thr His Leu Arg Ile Trp Ser Ala Glu
660 665 670Arg Leu Ala Asp Ala Ser Gly Ser Arg Asn Ile Ser Gly Ala Thr Trp
675 680 685Val Asn Tyr Phe Ala Arg Ser Leu Gly Val Lys Met Gly Lys Gly Val
690 695 700Asp Leu His Ser Leu Pro Pro Ile Thr Gly Leu Leu Thr Leu Gly Asn705 710 715 720Asn Val Ser Ile Glu Gln Glu Val Asp Leu Arg Gly Tyr Trp Leu Asp
725 730 735Gly Asp Ile Leu Arg Val Gly Thr Ile Glu Val His Asp Asn Ala Arg
740 745 750Ile Gly Ala Arg Ser Thr Leu Leu Pro Gly Thr Val Val Gly Thr Gly
755 760 765Ala His Leu Leu Pro Gly Ser Thr Val Thr Gly Asp Lys Thr Ile Lys
770 775 780Pro Gly Ser Arg Trp Ala Gly Ser Pro Ala Gln Lys Val Gly Arg Ala785 790 795 800Lys His Arg Phe Pro Thr Ser His Pro Pro Arg Arg Ser Arg Trp Val
805 810 815Pro Val Phe Gly Ala Thr Ser Ile Val Leu Ser Leu Leu Pro Leu Gln
820 825 830Ala Leu Ala Ile Gly Ala Ala Ile Thr Leu Trp Leu Ala Thr Ile Ser
835 840 845Pro Leu Pro Leu Ile Trp Gly Val Leu Val Phe Ala Thr Val Gly Ala
850 855 860Leu Ala Ala Phe Phe Ala Tyr Thr Val Thr Ile Trp Val Leu Val Arg865 870 875 880Leu Ile Gln Ile Gly Ile Lys Gly Gly Thr Ala Pro Val Arg Ser Arg
885 890 895Leu Gly Trp Gln Val Trp Ala Val Gln Arg Leu Met Asp Asp Ala Arg
900 905 910Thr Tyr Leu Phe Pro Leu Tyr Ala Ser Gln Leu Thr Pro Leu Trp Phe
915 920 925Arg Ser Leu Gly Ala Lys Ile Gly Lys Asp Val Glu Ile Ser Thr Ala
930 935 940Val Met Val Pro Lys Leu Ala Asp Ile Arg Glu Gly Ala Phe Leu Ala945 950 955 960Asp Asp Thr Leu Ile Gly Gly Tyr Glu Leu Gly Asn Gly Trp Leu Leu
965 970 975Ser Gly Glu Thr Arg Val Gly Lys Arg Ser Phe Ile Gly Asn Ser Gly
980 985 990Ile Ala Gly Pro Glu Arg Lys Leu Ala Lys Asn Ser Leu Val Ala Val
995 1000 1005Leu Ser Ser Thr Pro Lys Lys Ala Lys Ala Asn Ser Asn Trp Trp Gly
1010 1015 1020Ser Pro Pro Glu Arg Met Arg Arg Val Thr Val Glu Val Asp Glu Gly1025 1030 1035 1040Glu Ala Lys Thr Tyr Ser Pro Gly Phe Gly Val Lys Phe Ala Arg Gly
1045 1050 1055Ala Val Glu Thr Ala Arg Leu Leu Ala Pro Ile Thr Ser Gly Val Leu
1060 1065 1070Ala Ala Leu Ser Leu Leu Leu Met Gln Tyr Leu Leu Thr Glu Phe Asn
1075 1080 1085Met Trp Ile Thr Trp Leu Leu Gly Gly Leu Ile Leu Met Thr Val Gly
1090 1095 1100Val Leu Ala Met Gly Ile Thr Val Val Met Lys Trp Val Cys Val Gly1105 1110 1115 1120Lys His Lys Pro Ser Glu His Pro Leu Phe Ser Arg Phe Val Trp Leu
1125 1130 1135Asn Glu Leu Gln Asp Ala Phe Val Glu Ser Val Ala Gly Pro Trp Phe
1140 1145 1150Leu Val Pro Asn Leu Gly Thr Gly Ala Leu Asn Ala Gly Met Ser Ala
1155 1160 1165Leu Gly Ala His Ile Gly Arg Gly Ala Trp Ile Glu Ser Tyr Trp Leu
1170 1175 1180Pro Glu Thr Asp Leu Cys Tyr Ile Gly Lys Gly Ala Thr Val Gly Pro1185 1190 1195 1200Gly Val Val Val Gln Thr His Leu Phe Gln Asp Arg Val Met Ser Leu
1205 1210 1215Asp Thr Val Thr Val Ala Asp Gly Ala Thr Leu Ala Asp His Ser Val
1220 1225 1230Ala Leu Pro Ala Ser Leu Ile Asp Ala Ser Ala Thr Ile Gly Pro Gly
1235 1240 1245Ser Leu Val Met Arg Gly Asp Lys Val Pro Ala His Thr Arg Trp Gln
1250 1255 1260Gly Asn Pro Ile Glu Pro Trp Ser Asn Ser1265 1270<210>361<211>609<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(586)<223>RXN01997<400>361aaaaaggtgg gaaacttagc caatccaaag cccaaaaatg cgggttatgc tgcgctaacc 60tatgctgaca gccttgcgga agttgtgtac gttaggggcc atg aca atc aac gag 115
Met Thr Ile Asn Glu
1 5aag atc gca tca gct ttc aac aac caa gtg act gca gag ctt gaa gct 163Lys Ile Ala Ser Ala Phe Asn Asn Gln Val Thr Ala Glu Leu Glu Ala
10 15 20tca atg gtg tac ctt cag ctc tcc tac gtt cta gac gat ctg ggc ctc 211Ser Met Val Tyr Leu Gln Leu Ser Tyr Val Leu Asp Asp Leu Gly Leu
25 30 35acc ggc atg cgc gac tgg atg aag gca cag agc aaa gaa gag ctc gaa 259Thr Gly Met Arg Asp Trp Met Lys Ala Gln Ser Lys Glu Glu Leu Glu
40 45 50cac gca cag aag ttc gct cag cac ctt ctt gac cgt gac tac acc cca 307His Ala Gln Lys Phe Ala Gln His Leu Leu Asp Arg Asp Tyr Thr Pro
55 60 65cag atc ggt gac att gca cca cca aag ctt gat gtc acc tcc gct atc 355Gln Ile Gly Asp Ile Ala Pro Pro Lys Leu Asp Val Thr Ser Ala Ile70 75 80 85gag gct ttc gag gct tcc ctg gca cac gag cag aag atc tcc ggc ctg 403Glu Ala Phe Glu Ala Ser Leu Ala His Glu Gln Lys Ile Ser Gly Leu
90 95 100atc cgc gag ctc gct gcc atc cag gac gct gag aag gac tac gat tcc 451Ile Arg Glu Leu Ala Ala Ile Gln Asp Ala Glu Lys Asp Tyr Asp Ser
105 110 115cgc gca ctg atc gac tgg ttc ctc aac gag cag atc gaa gaa gaa gca 499Arg Ala Leu Ile Asp Trp Phe Leu Asn Glu Gln Ile Glu Glu Glu Ala
120 125 130acc gtc ggc gag atc atc gac cgc ctc cgt atc gct ggt gat tcc ggt 547Thr Val Gly Glu Ile Ile Asp Arg Leu Arg Ile Ala Gly Asp Ser Gly
135 140 145tcc gga atc ctg cgc atc gac ggc gaa ctc ggc tcc cgc taaattcccc 596Ser Gly Ile Leu Arg Ile Asp Gly Glu Leu Gly Ser Arg150 155 160gcagttttta atg 609<210>362<211>162<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>362Met Thr Ile Asn Glu Lys Ile Ala Ser Ala Phe Asn Asn Gln Val Thr1 5 10 15Ala Glu Leu Glu Ala Ser Met Val Tyr Leu Gln Leu Ser Tyr Val Leu
20 25 30Asp Asp Leu Gly Leu Thr Gly Met Arg Asp Trp Met Lys Ala Gln Ser
35 40 45Lys Glu Glu Leu Glu His Ala Gln Lys Phe Ala Gln His Leu Leu Asp
50 55 60Arg Asp Tyr Thr Pro Gln Ile Gly Asp Ile Ala Pro Pro Lys Leu Asp65 70 75 80Val Thr Ser Ala Ile Glu Ala Phe Glu Ala Ser Leu Ala His Glu Gln
85 90 95Lys Ile Ser Gly Leu Ile Arg Glu Leu Ala Ala Ile Gln Asp Ala Glu
100 105 110Lys Asp Tyr Asp Ser Arg Ala Leu Ile Asp Trp Phe Leu Asn Glu Gln
115 120 125Ile Glu Glu Glu Ala Thr Val Gly Glu Ile Ile Asp Arg Leu Arg Ile
l30 135 140Ala Gly Asp Ser Gly Ser Gly Ile Leu Arg Ile Asp Gly Glu Leu Gly145 150 155 160Ser Arg<210>363<211>867<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(844)<223>RXA01848<400>363ctgcaaggaa ctgcgcaggc gaaggcgcag actactggaa aggtaggtac tgccggatcc 60ggcgacccct ttcgctccta ggcatttgcg cctggcgtcc atg ggg gag gag gac 115
Met Gly Glu Glu Asp
1 5tcc acc cca ggt agg cgt tcc aag gcg tat tcg cgc cag ggc gct gat 163Ser Thr Pro Gly Arg Arg Ser Lys Ala Tyr Ser Arg Gln Gly Ala Asp
10 15 20gtc cgc ccc atg aag ggt gga cac ggc atc aac tta gtg ggc acg ctc 211Val Arg Pro Met Lys Gly Gly His Gly Ile Asn Leu Val Gly Thr Leu
25 30 35atg gcg gct acg gaa cgc ggc gcc aac att gtt gaa ggc gtg gtc gat 259Met Ala Ala Thr Glu Arg Gly Ala Asn Ile Val Glu Gly Val Val Asp
40 45 50ttc cgg ccc acg gac ctg cgg ggt tcg ctg cgc cgt ggg cgc gaa gcc 307Phe Arg Pro Thr Asp Leu Arg Gly Ser Leu Arg Arg Gly Arg Glu Ala
55 60 65aac ctc atc gtg ttc gtc gtc gac aca tcg ggg tcg atg gct gcg cgt 355Asn Leu Ile Val Phe Val Val Asp Thr Ser Gly Ser Met Ala Ala Arg70 75 80 85tcc agg gtg cgt gcg gtc acc ggg act att acc tct atg ctt aac gac 403Ser Arg Val Arg Ala Val Thr Gly Thr Ile Thr Ser Met Leu Asn Asp
90 95 100gcc tac cag cgc cgc gac aag gtt gcg gtt atc gcg gtc aac ggc aac 451Ala Tyr Gln Arg Arg Asp Lys Val Ala Val Ile Ala Val Asn Gly Asn
105 110 115aag ccg aca ctg gtg ttg aat cca aca aat tct gtg gag caa gct cag 499Lys Pro Thr Leu Val Leu Asn Pro Thr Asn Ser Val Glu Gln Ala Gln
120 125 130cag aaa tta aag gat atg ccg atg ggt ggt cgc act cca ctg gca gag 547Gln Lys Leu Lys Asp Met Pro Met Gly Gly Arg Thr Pro Leu Ala Glu
135 140 145ggg ctg ctc atg gcc aag gat ctc atg gca agg gaa ctc cga aag gaa 595Gly Leu Leu Met Ala Lys Asp Leu Met Ala Arg Glu Leu Arg Lys Glu150 155 160 165ccc ggc cga cgc gcg atc ctc atg gtg atg acc gat ggc caa gac acc 643Pro Gly Arg Arg Ala Ile Leu Met Val Met Thr Asp Gly Gln Asp Thr
170 175 180tcc gat gcc ggc gaa gca ggc att gcc acc gcg gcg gaa aca gtg gtg 691Ser Asp Ala Gly Glu Ala Gly Ile Ala Thr Ala Ala Glu Thr Val Val
185 190 195aaa tca cga ctg tcc ggc aac gtg gtc atc gac tgc gaa ggc cga ctc 739Lys Ser Arg Leu Ser Gly Asn Val Val Ile Asp Cys Glu Gly Arg Leu
200 205 210aaa gtg cgc aaa gag cgc gcc ggg gtg ttg gct gaa atg ctc ggt ggt 787Lys Val Arg Lys Glu Arg Ala Gly Val Leu Ala Glu Met Leu Gly Gly
215 220 225gtg tgc gtg aga ttg cgt gat ctt aac tcc gag cac atc aaa atg gtg 835Val Cys Val Arg Leu Arg Asp Leu Asn Ser Glu His Ile Lys Met Val230 235 240 245att aac gcc tagacaacca gagtgagggt ttc 867Ile Asn Ala<210>364<211>248<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>364Met Gly Glu Glu Asp Ser Thr Pro Gly Arg Arg Ser Lys Ala Tyr Ser1 5 10 15Arg Gln Gly Ala Asp Val Arg Pro Met Lys Gly Gly His Gly Ile Asn
20 25 30Leu Val Gly Thr Leu Met Ala Ala Thr Glu Arg Gly Ala Asn Ile Val
35 40 45Glu Gly Val Val Asp Phe Arg Pro Thr Asp Leu Arg Gly Ser Leu Arg
50 55 60Arg Gly Arg Glu Ala Asn Leu Ile Val Phe Val Val Asp Thr Ser Gly65 70 75 80Ser Met Ala Ala Arg Ser Arg Val Arg Ala Val Thr Gly Thr Ile Thr
85 90 95Ser Met Leu Asn Asp Ala Tyr Gln Arg Arg Asp Lys Val Ala Val Ile
100 105 110Ala Val Asn Gly Asn Lys Pro Thr Leu Val Leu Asn Pro Thr Asn Ser
115 120 125Val Glu Gln Ala Gln Gln Lys Leu Lys Asp Met Pro Met Gly Gly Arg
130 135 140Thr Pro Leu Ala Glu Gly Leu Leu Met Ala Lys Asp Leu Met Ala Arg145 150 155 160Glu Leu Arg Lys Glu Pro Gly Arg Arg Ala Ile Leu Met Val Met Thr
165 170 175Asp Gly Gln Asp Thr Ser Asp Ala Gly Glu Ala Gly Ile Ala Thr Ala
180 185 190Ala Glu Thr Val Val Lys Ser Arg Leu Ser Gly Asn Val Val Ile Asp
195 200 205Cys Glu Gly Arg Leu Lys Val Arg Lys Glu Arg Ala Gly Val Leu Ala
210 215 220Glu Met Leu Gly Gly Val Cys Val Arg Leu Arg Asp Leu Asn Ser Glu225 230 235 240His Ile Lys Met Val Ile Asn Ala
245<210>365<211>1224<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1201)<223>RXN01849<400>365aaaaccttaa gttgggtggt taaacccact aaggtctcac tttatggatg tgccaggtca 60caccaaaaaa tctcaagaaa actcacatta aaggacagta atg gcg tca caa cag 115
Met Ala Ser Gln Gln
1 5atc cgc tat cea ttc tcc gcg gtt gtg gga caa gac gag ctt cgg ctt 163Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln Asp Glu Leu Arg Leu
10 15 20gcg ttg atc ctc act gcg att tcc cca cgc att ggt ggc gtg gtg att 211Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile Gly Gly Val Val Ile
25 30 35cga ggt gag aag ggt aca gcg aaa act acc act gtg cgt gct ttt gct 259Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr Val Arg Ala Phe Ala
40 45 50ggt ctt tta ggt gat gcc cct ttg gtg aac ttg cct ctc gga tcc acg 307Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu Pro Leu Gly Ser Thr
55 60 65gag gat cgt gtg gtg ggt tcc ctc aac atg gaa act gtg ttg acc acc 355Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu Thr Val Leu Thr Thr70 75 80 85ggc cgt gcg gaa tat cag cca ggt ttg ctc gcg cag gct gat ggc ggt 403Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala Gln Ala Asp Gly Gly
90 95 100gtg ctg tat gtc gat gag gtc aac ctc ttg gcg gat cac ctg gtg gat 451Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala Asp His Leu Val Asp
105 110 115gct ctg ctc gat gca gct gca agc ggt cgc gtc agc att gag cgt gac 499Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val Ser Ile Glu Arg Asp
120 125 130ggt att tcg cat tct tca cca gca aac ttt gtg ttg gtg ggc acc atg 547Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val Leu Val Gly Thr Met
135 140 145aat ccg gag gaa ggc gag ctg cgc ccg cag ctg ctg gac cgt ttc ggt 595Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu Leu Asp Arg Phe Gly150 155 160 165ttg gct gtg gac gtt gct gcg tct acg aac cct gag gtg cgc gtg gag 643Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro Glu Val Arg Val Glu
170 175 180atc att cgc cgc cgg ctt gat ttt gaa aac gct cct gag cag ttc atg 691Ile Ile Arg Arg Arg Leu Asp Phe Glu Asn Ala Pro Glu Gln Phe Met
185 190 195gct aag tgg gct gag caa gat gcg gac acc tcc aac cgt att ttg gcg 739Ala Lys Trp Ala Glu Gln Asp Ala Asp Thr Ser Asn Arg Ile Leu Ala
200 205 210gct aag gat ttg ctg cct ggt gtg gag ctg ccg gat ctg atc ttg tcg 787Ala Lys Asp Leu Leu Pro Gly Val Glu Leu Pro Asp Leu Ile Leu Ser
215 220 225cag att gcg tgg ttg tgt gca cgt att gaa gtc gac ggt atg cgc gct 835Gln Ile Ala Trp Leu Cys Ala Arg Ile Glu Val Asp Gly Met Arg Ala230 235 240 245gac ctg gtg atc acg cgt acc gca ctt gct cac gcc gcg tgg gct gga 883Asp Leu Val Ile Thr Arg Thr Ala Leu Ala His Ala Ala Trp Ala Gly
250 255 260cgc act gtg gtt acg gaa gaa gac gtg gag atc gca gct cgc cta gcg 931Arg Thr Val Val Thr Glu Glu Asp Val Glu Ile Ala Ala Arg Leu Ala
265 270 275ttg ccg cac cgc cgt cgc cgt aat cct ttc gat gct cca gaa atg gag 979Leu Pro His Arg Arg Arg Arg Asn Pro Phe Asp Ala Pro Glu Met Glu
280 285 290gag cgc aag ctt cag gaa acc ctg cag gaa gct cgg gac ttc ttc aaa 1027Glu Arg Lys Leu Gln Glu Thr Leu Gln Glu Ala Arg Asp Phe Phe Lys
295 300 305gac aat gaa gat aaa gga cct gcc gcc aag atc acc gat gag gaa acc 1075Asp Asn Glu Asp Lys Gly Pro Ala Ala Lys Ile Thr Asp Glu Glu Thr310 315 320 325ggt gca gag gcc ttt acc gat acc gac aat ccc acc gag gaa gac ggt 1123Gly Ala Glu Ala Phe Thr Asp Thr Asp Asn Pro Thr Glu Glu Asp Gly
330 335 340ctg caa gga act gcg cag gcg aag gcg cag act act gga aag gta ggt 1171Leu Gln Gly Thr Ala Gln Ala Lys Ala Gln Thr Thr Gly Lys Val Gly
345 350 355act gcc gga tcc ggc gac ccc ttt cgc tcc taggcatttg cgcctggcgt 1221Thr Ala Gly Ser Gly Asp Pro Phe Arg Ser
360 365cca1224<210>366<211>367<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>366Met Ala Ser Gln Gln Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln1 5 10 15Asp Glu Leu Arg Leu Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile
20 25 30Gly Gly Val Val Ile Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr
35 40 45Val Arg Ala Phe Ala Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu
50 55 60Pro Leu Gly Ser Thr Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu65 70 75 80Thr Val Leu Thr Thr Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala
85 90 95Gln Ala Asp Gly Gly Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala
100 105 110Asp His Leu Val Asp Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val
115 120 125Ser Ile Glu Arg Asp Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val
130 135 140Leu Val Gly Thr Met Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu145 150 155 160Leu Asp Arg Phe Gly Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro
165 170 175Glu Val Arg Val Glu Ile Ile Arg Arg Arg Leu Asp Phe Glu Asn Ala
180 185 190Pro Glu Gln Phe Met Ala Lys Trp Ala Glu Gln Asp Ala Asp Thr Ser
195 200 205Asn Arg Ile Leu Ala Ala Lys Asp Leu Leu Pro Gly Val Glu Leu Pro
210 215 220Asp Leu Ile Leu Ser Gln Ile Ala Trp Leu Cys Ala Arg Ile Glu Val225 230 235 240Asp Gly Met Arg Ala Asp Leu Val Ile Thr Arg Thr Ala Leu Ala His
245 250 255Ala Ala Trp Ala Gly Arg Thr Val Val Thr Glu Glu Asp Val Glu Ile
260 265 270Ala Ala Arg Leu Ala Leu Pro His Arg Arg Arg Arg Asn Pro Phe Asp
275 280 285Ala Pro Glu Met Glu Glu Arg Lys Leu Gln Glu Thr Leu Gln Glu Ala
290 295 300Arg Asp Phe Phe Lys Asp Asn Glu Asp Lys Gly Pro Ala Ala Lys Ile305 310 315 320Thr Asp Glu Glu Thr Gly Ala Glu Ala Phe Thr Asp Thr Asp Asn Pro
325 330 335Thr Glu Glu Asp Gly Leu Gln Gly Thr Ala Gln Ala Lys Ala Gln Thr
340 345 350Thr Gly Lys Val Gly Thr Ala Gly Ser Gly Asp Pro Phe Arg Ser
355 360 365<210>367<211>473<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(450)<223>FRXA01849<400>367ctg cct ggt gtg gag ctg ccg gat ctg atc ttg tcg cag att gcg tgg 48Leu Pro Gly Val Glu Leu Pro Asp Leu Ile Leu Ser Gln Ile Ala Trp1 5 10 15ttg tgt gca cgt att gaa gtc gac ggt atg cgc gct gac ctg gtg atc 96Leu Cys Ala Arg Ile Glu Val Asp Gly Met Arg Ala Asp Leu Val Ile
20 25 30acg cgt acc gca ctt gct cac gcc gcg tgg gct gga cgc act gtg gtt 144Thr Arg Thr Ala Leu Ala His Ala Ala Trp Ala Gly Arg Thr Val Val
35 40 45acg gaa gaa gac gtg gag atc gca gct cgc cta gcg ttg ccg cac cgc 192Thr Glu Glu Asp Val Glu Ile Ala Ala Arg Leu Ala Leu Pro His Arg
50 55 60cgt cgc cgt aat cct ttc gat gct cca gaa atg gag gag cgc aag ctt 240Arg Arg Arg Asn Pro Phe Asp Ala Pro Glu Met Glu Glu Arg Lys Leu65 70 75 80cag gaa acc ctg cag gaa gct cgg gac ttc ttc aaa gac aat gaa gat 288Gln Glu Thr Leu Gln Glu Ala Arg Asp Phe Phe Lys Asp Asn Glu Asp
85 90 95aaa gga cct gcc gcc aag atc acc gat gag gaa acc ggt gca gag gcc 336Lys Gly Pro Ala Ala Lys Ile Thr Asp Glu Glu Thr Gly Ala Glu Ala
100 105 110ttt acc gat agc gac aat ccc acc gag gaa gac ggt ctg caa gga act 384Phe Thr Asp Thr Asp Asn Pro Thr Glu Glu Asp Gly Leu Gln Gly Thr
115 120 125gcg cag gcg aag gcg cag act act gga aag gta ggt act gcc gga tcc 432Ala Gln Ala Lys Ala Gln Thr Thr Gly Lys Val Gly Thr Ala Gly Ser
130 135 140ggc gac ccc ttt cgc tcc taggcatttg cgcctggcgt cca 473Gly Asp Pro Phe Arg Ser145 150<210>368<211>150<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>368Leu Pro Gly Val Glu Leu Pro Asp Leu Ile Leu Ser Gln Ile Ala Trp1 5 10 15Leu Cys Ala Arg Ile Glu Val Asp Gly Met Arg Ala Asp Leu Val Ile
20 25 30Thr Arg Thr Ala Leu Ala His Ala Ala Trp Ala Gly Arg Thr Val Val
35 40 45Thr Glu Glu Asp Val Glu Ile Ala Ala Arg Leu Ala Leu Pro His Arg
50 55 60Arg Arg Arg Asn Pro Phe Asp Ala Pro Glu Met Glu Glu Arg Lys Leu65 70 75 80Gln Glu Thr Leu Gln Glu Ala Arg Asp Phe Phe Lys Asp Asn Glu Asp
85 90 95Lys Gly Pro Ala Ala Lys Ile Thr Asp Glu Glu Thr Gly Ala Glu Ala
100 105 110Phe Thr Asp Thr Asp Asn Pro Thr Glu Glu Asp Gly Leu Gln Gly Thr
115 120 125Ala Gln Ala Lys Ala Gln Thr Thr Gly Lys Val Gly Thr Ala Gly Ser
130 135 140Gly Asp Pro Phe Arg Ser145 150<210>369<211>667<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(667)<223>FRXA01691<400>369aaaaccttaa gttgggtggt taaacccact aaggtctcac tttatggatg tgccaggtca 60caccaaaaaa tctcaagaaa actcacatta aaggacagta atg gcg tca caa cag 115
Met Ala Ser Gln Gln
1 5atc cgc tat cca ttc tcc gcg gtt gtg gga caa gac gag ctt cgg ctt 163Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln Asp Glu Leu Arg Leu
10 15 20gcg ttg atc ctc act gcg att tcc cca cgc att ggt ggc gtg gtg att 211Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile Gly Gly Val Val Ile
25 30 35cga ggt gag aag ggt aca gcg aaa act acc act gtg cgt gct ttt gct 259Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr Val Arg Ala Phe Ala
40 45 50ggt ctt tta ggt gat gcc cct ttg gtg aac ttg cct ctc gga tcc acg 307Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu Pro Leu Gly Ser Thr
55 60 65gag gat cgt gtg gtg ggt tcc ctc aac atg gaa act gtg ttg acc acc 355Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu Thr Val Leu Thr Thr70 75 80 85ggc cgt gcg gaa tat cag cca ggt ttg ctc gcg cag gct gat ggc ggt 403Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala Gln Ala Asp Gly Gly
90 95 100gtg ctg tat gtc gat gag gtc aac ctc ttg gcg gat cac ctg gtg gat 451Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala Asp His Leu Val Asp
105 110 115gct ctg ctc gat gca gct gca agc ggt cgc gtc agc att gag cgt gac 499Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val Ser Ile Glu Arg Asp
120 125 130ggt att tcg cat tct tca cca gca aac ttt gtg ttg gtg ggc acc atg 547Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val Leu Val Gly Thr Met
135 140 145aat ccg gag gaa ggc gag ctg cgc ccg cag ctg ctg gac cgt ttc ggt 595Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu Leu Asp Arg Phe Gly150 155 160 165ttg gct gtg gac gtt gct gcg tct acg aac cct gag gtg cgc gtg gag 643Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro Glu Val Arg Val Glu
170 175 180atc att cgc cgc cgg ctt gat ttt 667Ile Ile Arg Arg Arg Leu Asp Phe
185<210>370<211>189<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>370Met Ala Ser Gln Gln Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln1 5 10 15Asp Glu Leu Arg Leu Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile
20 25 30Gly Gly Val Val Ile Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr
35 40 45Val Arg Ala Phe Ala Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu
50 55 60Pro Leu Gly Ser Thr Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu65 70 75 80Thr Val Leu Thr Thr Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala
85 90 95Gln Ala Asp Gly Gly Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala
100 105 110Asp His Leu Val Asp Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val
115 120 125Ser Ile Glu Arg Asp Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val
130 135 140Leu Val Gly Thr Met Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu145 150 155 160Leu Asp Arg Phe Gly Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro
165 170 175Glu Val Arg Val Glu Ile Ile Arg Arg Arg Leu Asp Phe
180 185<210>371<211>601<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(601)<223>RXN00665<400>371accaaacact tctgtgcgtg acacgcgcca ccttatactc ccacaagcaa cacagaacac 60tcgggatctc aaagtttcga gaaacacaga aagggcagca atg agc agc tca aca 115
Met Ser Ser Ser Thr
1 5ctt ctc ctg gct tca gga caa gtc acg gca tta gcc gct gac tac acg 163Leu Leu Leu Ala Ser Gly Gln Val Thr Ala Leu Ala Ala Asp Tyr Thr
10 15 20ctc agc cac acc ccc tca gat ggc atc ctg gta gtc ctt ggc ttc gcc 211Leu Ser His Thr Pro Ser Asp Gly Ile Leu Val Val Leu Gly Phe Ala
25 30 35atg atc ctc acc ttc atg acc ctg atc atg ctg ggt cga ctc acc cca 259Met Ile Leu Thr Phe Met Thr Leu Ile Met Leu Gly Arg Leu Thr Pro
40 45 50atg gtg gcc atg ctg ttg gtc ccc acc atc ttc ggt ctc atc gcc ggc 307Met Val Ala Met Leu Leu Val Pro Thr Ile Phe Gly Leu Ile Ala Gly
55 60 65gca gga ctc ggc ctt ggt gac atg gcg ctt gac gcc atc aag gac atg 355Ala Gly Leu Gly Leu Gly Asp Met Ala Leu Asp Ala Ile Lys Asp Met70 75 80 85gcg cct acc gcg gca ctc ctg atg ttc gcg att atg ttc ttc gga atc 403Ala Pro Thr Ala Ala Leu Leu Met Phe Ala Ile Met Phe Phe Gly Ile
90 95 100atg atc gac gtc gga ctc ttc gac ccc ctg atc cgc gtg atc acc cgc 451Met Ile Asp Val Gly Leu Phe Asp Pro Leu Ile Arg Val Ile Thr Arg
105 110 115gtt ctt cac gat gac ccc gca aag gtc gtc atc ggc acc gca gta ctt 499Val Leu His Asp Asp Pro Ala Lys Val Val Ile Gly Thr Ala Val Leu
120 125 130gca ggt gtt gtc tcc ctc gac ggc gac ggc tcc acc acc ttc atc att 547Ala Gly Val Val Ser Leu Asp Gly Asp Gly Ser Thr Thr Phe Ile Ile
135 140 145acc acc ttc cgc gat gct gcc cat cta cct gcg cct tgg cat gag ccc 595Thr Thr Phe Arg Asp Ala Ala His Leu Pro Ala Pro Trp His Glu Pro150 155 160 165tgt ggt 601Cys Gly<210>372<211>167<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>372Met Ser Ser Ser Thr Leu Leu Leu Ala Ser Gly Gln Val Thr Ala Leu1 5 10 15Ala Ala Asp Tyr Thr Leu Ser His Thr Pro Ser Asp Gly Ile Leu Val
20 25 30Val Leu Gly Phe Ala Met Ile Leu Thr Phe Met Thr Leu Ile Met Leu
35 40 45Gly Arg Leu Thr Pro Met Val Ala Met Leu Leu Val Pro Thr Ile Phe
50 55 60Gly Leu Ile Ala Gly Ala Gly Leu Gly Leu Gly Asp Met Ala Leu Asp65 70 75 80Ala Ile Lys Asp Met Ala Pro Thr Ala Ala Leu Leu Met Phe Ala Ile
85 90 95Met Phe Phe Gly Ile Met Ile Asp Val Gly Leu Phe Asp Pro Leu Ile
100 105 110Arg Val Ile Thr Arg Val Leu His Asp Asp Pro Ala Lys Val Val Ile
115 120 125Gly Thr Ala Val Leu Ala Gly Val Val Ser Leu Asp Gly Asp Gly Ser
130 135 140Thr Thr Phe Ile Ile Thr Thr Phe Arg Asp Ala Ala His Leu Pro Ala145 150 155 160Pro Trp His Glu Pro Cys Gly
165<210>373<211>390<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(367)<223>RXN03026<400>373gttggcggcg cagtatcgtg aggtgcggga cctcgagcgg ggaatcccaa actagcatcc 60cgaactagcc ccccaacaac aattagaaat ggaacctaaa atg cct gga aaa att 115
Met Pro Gly Lys Ile
1 5ctc ctt ctc aac ggc cca aac ctg aac atg ctg ggc aaa cgc gag cct 163Leu Leu Leu Asn Gly Pro Asn Leu Asn Met Leu Gly Lys Arg Glu Pro
10 15 20gac att tac gga cac gac acc ttg gaa gac gtc gtc gcg ctg gca acc 211Asp Ile Tyr Gly His Asp Thr Leu Glu Asp Val Val Ala Leu Ala Thr
25 30 35gct gag gct gcg aaa cac ggc ctt gag gtt gag gcg ctg cag agc aat 259Ala Glu Ala Ala Lys His Gly Leu Glu Val Glu Ala Leu Gln Ser Asn
40 45 50cac caa ggt gag cta atc gat gcg ctg cac aac gct cgc ggg acc cac 307His Gln Gly Glu Leu Ile Asp Ala Leu His Asn Ala Arg Gly Thr His
55 60 65atc ggt tgc gtg att aac ccc ggc ggc ctg act aca ctt cgg tgg cgc 355Ile Gly Cys Val Ile Asn Pro Gly Gly Leu Thr Thr Leu Arg Trp Arg70 75 80 85ttt tgg atg ctg tgaaggcgtc tgagcttcct acc 390Phe Trp Met Leu<210>374<211>89<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>374Met Pro Gly Lys Ile Leu Leu Leu Asn Gly Pro Asn Leu Asn Met Leu1 5 10 15Gly Lys Arg Glu Pro Asp Ile Tyr Gly His Asp Thr Leu Glu Asp Val
20 25 30Val Ala Leu Ala Thr Ala Glu Ala Ala Lys His Gly Leu Glu Val Glu
35 40 45Ala Leu Gln Ser Asn His Gln Gly Glu Leu Ile Asp Ala Leu His Asn
50 55 60Ala Arg Gly Thr His Ile Gly Cys Val Ile Asn Pro Gly Gly Leu Thr65 70 75 80Thr Leu Arg Trp Arg Phe Trp Met Leu
85<210>375<211>384<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(361)<223>RXN02908<400>375gccaacgagg gttggtttac cacctctgat tcaggtgaac tccacgacgg gattctcacc 60gtgactggtc gcgtggatac ccgtcattga ttccggtgga ttg aag ttg cac cca 115
Leu Lys Leu His Pro
1 5gag gta ctg gaa cgt gcc atc gca gat att aaa ggt gtc acc gcg gcg 163Glu Val Leu Glu Arg Ala Ile Ala Asp Ile Lys Gly Val Thr Ala Ala
10 15 20tgt gtt gtg ggt att ccc gat ccc cga tta ggc caa gca att gtg gcc 211Cys Val Val Gly Ile Pro Asp Pro Arg Leu Gly Gln Ala Ile Val Ala
25 30 35gcg tac tcc gga tcg atc agt ccg tct gaa gtt att gaa ggc ctc gac 259Ala Tyr Ser Gly Ser Ile Ser Pro Ser Glu Val Ile Glu Gly Leu Asp
40 45 50gat cta cct cgt tgg cag ctt ccc aaa egg ctg aag cat ctg gaa tct 307Asp Leu Pro Arg Trp Gln Leu Pro Lys Arg Leu Lys His Leu Glu Ser
55 60 65ttg ccc agc att ggt cct gga aaa gct gat cga cgt gct atc gcg aag 355Leu Pro Ser Ile Gly Pro Gly Lys Ala Asp Arg Arg Ala Ile Ala Lys70 75 80 85ctg ttt tagtcttcat tcttgctggc tgc 384Leu Phe<210>376<211>87<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>376Leu Lys Leu His Pro Glu Val Leu Glu Arg Ala Ile Ala Asp Ile Lys1 5 10 15Gly Val Thr Ala Ala Cys Val Val Gly Ile Pro Asp Pro Arg Leu Gly
20 25 30Gln Ala Ile Val Ala Ala Tyr Ser Gly Ser Ile Ser Pro Ser Glu Val
35 40 45Ile Glu Gly Leu Asp Asp Leu Pro Arg Trp Gln Leu Pro Lys Arg Leu
50 55 60Lys His Leu Glu Ser Leu Pro Ser Ile Gly Pro Gly Lys Ala Asp Arg65 70 75 80Arg Ala Ile Ala Lys Leu Phe
85<210>377<211>667<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(667)<223>RXN03000<400>377cagcgtgttg ctgcgcctgc agaaaaccta cagcgtcacc cacaaagtcg aaatccaagg 60ctaaccccct ttttcaactc acagttagga aacttcaccc atg tct ctt cca cat 115
Met Ser Leu Pro His
1 5tct gat gaa ctc cgc ggc caa aag atc att att tcc ggt ggc gga att 163Ser Asp Glu Leu Arg Gly Gln Lys Ile Ile Ile Ser Gly Gly Gly Ile
10 15 20ggt ggc gca gca ggt gca ctt gcg ctt gct ttg cgc ggt gcc gat gtc 211Gly Gly Ala Ala Gly Ala Leu Ala Leu Ala Leu Arg Gly Ala Asp Val
25 30 35act ttg tac gaa cgc gca gct gag ttc aag gag gtc ggc gct ggc ctc 259Thr Leu Tyr Glu Arg Ala Ala Glu Phe Lys Glu Val Gly Ala Gly Leu
40 45 50cag atc ggt ccg cac ggc tgg cga atg ctg gaa tcc tgg ggt ctg ctc 307Gln Ile Gly Pro His Gly Trp Arg Met Leu Glu Ser Trp Gly Leu Leu
55 60 65gac caa att gtc gtg gcc ggc tac ctc cca gaa gac atg cag ttc cgc 355Asp Gln Ile Val Val Ala Gly Tyr Leu Pro Glu Asp Met Gln Phe Arg70 75 80 85gac gct gtc aac cgc gaa acc atc ctg acc atg cgt ttc gat gaa gaa 403Asp Ala Val Asn Arg Glu Thr Ile Leu Thr Met Arg Phe Asp Glu Glu
90 95 100ttc cag cag cac tac ggc ggt cgc tac ctg gtg att cac cgc tct gac 451Phe Gln Gln His Tyr Gly Gly Arg Tyr Leu Val Ile His Arg Ser Asp
105 110 115ctg ctc aac atc ctg gtc acc aac gcc gaa gca gcg ggc gcg aag ctc 499Leu Leu Asn Ile Leu Val Thr Asn Ala Glu Ala Ala Gly Ala Lys Leu
120 125 130cac aat ggc gtc ctg gtc acc gat tcc cgc acc gtc gac ggc ggt atc 547His Asn Gly Val Leu Val Thr Asp Ser Arg Thr Val Asp Gly Gly Ile
135 140 145gag gtg gac atc gaa tcc tcc atc aac aag ggc gaa gat aac aag act 595Glu Val Asp Ile Glu Ser Ser Ile Asn Lys Gly Glu Asp Asn Lys Thr150 155 160 165ttg ctt gtc gac gcc ttc ctc gcc ttc gac ggc atc cac tcg gtc atg 643Leu Leu Val Asp Ala Phe Leu Ala Phe Asp Gly Ile His Ser Val Met
170 175 180cgc aaa aag ctt gtc gac gac gcc 667Arg Lys Lys Leu Val Asp Asp Ala
185<210>378<211>189<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>378Met Ser Leu Pro His Ser Asp Glu Leu Arg Gly Gln Lys Ile Ile Ile1 5 10 15Ser Gly Gly Gly Ile Gly Gly Ala Ala Gly Ala Leu Ala Leu Ala Leu
20 25 30Arg Gly Ala Asp Val Thr Leu Tyr Glu Arg Ala Ala Glu Phe Lys Glu
35 40 45Val Gly Ala Gly Leu Gln Ile Gly Pro His Gly Trp Arg Met Leu Glu
50 55 60Ser Trp Gly Leu Leu Asp Gln Ile Val Val Ala Gly Tyr Leu Pro Glu65 70 75 80Asp Met Gln Phe Arg Asp Ala Val Asn Arg Glu Thr Ile Leu Thr Met
85 90 95Arg Phe Asp Glu Glu Phe Gln Gln His Tyr Gly Gly Arg Tyr Leu Val
100 105 110Ile His Arg Ser Asp Leu Leu Asn Ile Leu Val Thr Asn Ala Glu Ala
115 120 125Ala Gly Ala Lys Leu His Asn Gly Val Leu Val Thr Asp Ser Arg Thr
130 135 140Val Asp Gly Gly Ile Glu Val Asp Ile Glu Ser Ser Ile Asn Lys Gly145 150 155 160Glu Asp Asn Lys Thr Leu Leu Val Asp Ala Phe Leu Ala Phe Asp Gly
165 170 175IIe His Ser Val Met Arg Lys Lys Leu Val Asp Asp Ala
180 185<210>379<211>766<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(766)<223>RXN03036<400>379tagaaaaatc tacccagtaa gcattcagga accattcaga atcttttctt agcatgtctc 60tatcagcgta aacgtccgaa catgaaaggc tagaaaagcc atg gct gag cag ttg 115
Met Ala Glu Gln Leu
1 5cgt caa ttt gaa ggc agg gtc ctc cct aat caa tcc gag gac ttg gaa 163Arg Gln Phe Glu Gly Arg Val Leu Pro Asn Gln Ser Glu Asp Leu Glu
10 15 20gat cag ggt ttg gga ttt gac ctg gga acc gtt ttc tcc cgc agg aag 211Asp Gln Gly Leu Gly Phe Asp Leu Gly Thr Val Phe Ser Arg Arg Lys
25 30 35gtt ttg gga ttc atc ggt gtt ggt gga gca ggt gtg gca ctt gct gct 259Val Leu Gly Phe Ile Gly Val Gly Gly Ala Gly Val Ala Leu Ala Ala
40 45 50tgt tca cct tct ggt tct tcc gcg gca tcg agc acc tca agc gcg tcc 307Cys Ser Pro Ser Gly Ser Ser Ala Ala Ser Ser Thr Ser Ser Ala Ser
55 60 65agc agc gca gct gca acc acc agt gca gca gca gag act ttg act gag 355Ser Ser Ala Ala Ala Thr Thr Ser Ala Ala Ala Glu Thr Leu Thr Glu70 75 80 85atg aag tcg gag act gct ggt ccg tac ccg ggc gat ggt tcg aat ggt 403Met Lys Ser Glu Thr Ala Gly Pro Tyr Pro Gly Asp Gly Ser Asn Gly
90 95 100ccg gat gtg ttg gag gtc tcc ggt gtg gag cgc cag gac atc acc aag 451Pro Asp Val Leu Glu Val Ser Gly Val Glu Arg Gln Asp Ile Thr Lys
105 110 115tcg att gat tct gac acc gtg gca gag ggc gta cct ctg acg ttg act 499Ser Ile Asp Ser Asp Thr Val Ala Glu Gly Val Pro Leu Thr Leu Thr
120 125 130atg acc att ttg gac atg aac aac aac aat cag cca atg gag ggt gct 547Met Thr Ile Leu Asp Met Asn Asn Asn Asn Gln Pro Met Glu Gly Ala
135 140 145gcg gtg tac gtg tgg cac tgt gat gcg ccg ggt cga tat tcg atg tac 595Ala Val Tyr Val Trp His Cys Asp Ala Pro Gly Arg Tyr Ser Met Tyr150 155 160 165gac tct gag ctg gaa gat gag acc tat tta cgc ggt gtg cag att acc 643Asp Ser Glu Leu Glu Asp Glu Thr Tyr Leu Arg Gly Val Gln Ile Thr
170 175 180gat aag tat ggc cag gtc acg ttc gat acc att ttc cct ggt tgt tat 691Asp Lys Tyr Gly Gln Val Thr Phe Asp Thr Ile Phe Pro Gly Cys Tyr
185 190 195gcg ggc cgt tgg gtg cat att cat ttc gag gtg ttc ccg gat cga gac 739Ala Gly Arg Trp Val His Ile His Phe Glu Val Phe Pro Asp Arg Asp
200 205 210agc atc acg gat tcc acg aac aac att 766Ser Ile Thr Asp Ser Thr Asn Asn Ile
215 220<210>380<211>222<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>380Met Ala Glu Gln Leu Arg Gln Phe Glu Gly Arg Val Leu Pro Asn Gln1 5 10 15Ser Glu Asp Leu Glu Asp Gln Gly Leu Gly Phe Asp Leu Gly Thr Val
20 25 30Phe Ser Arg Arg Lys Val Leu Gly Phe Ile Gly Val Gly Gly Ala Gly
35 40 45Val Ala Leu Ala Ala Cys Ser Pro Ser Gly Ser Ser Ala Ala Ser Ser
50 55 60Thr Ser Ser Ala Ser Ser Ser Ala Ala Ala Thr Thr Ser Ala Ala Ala65 70 75 80Glu Thr Leu Thr Glu Met Lys Ser Glu Thr Ala Gly Pro Tyr Pro Gly
85 90 95Asp Gly Ser Asn Gly Pro Asp Val Leu Glu Val Ser Gly Val Glu Arg
100 105 110Gln Asp Ile Thr Lys Ser Ile Asp Ser Asp Thr Val Ala Glu Gly Val
115 120 125Pro Leu Thr Leu Thr Met Thr Ile Leu Asp Met Asn Asn Asn Asn Gln
130 135 140Pro Met Glu Gly Ala Ala Val Tyr Val Trp His Cys Asp Ala Pro Gly145 150 155 160Arg Tyr Ser Met Tyr Asp Ser Glu Leu Glu Asp Glu Thr Tyr Leu Arg
165 170 175Gly Val Gln Ile Thr Asp Lys Tyr Gly Gln Val Thr Phe Asp Thr Ile
180 185 190Phe Pro Gly Cys Tyr Ala Gly Arg Trp Val His Ile His Phe Glu Val
195 200 205Phe Pro Asp Arg Asp Ser Ile Thr Asp Ser Thr Asn Asn Ile
210 215 220<210>381<211>318<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(295)<223>RXN02974<400>381gcaggcatgg acacattcca ggtcctgacc ggcgtcagcg gctactacga tttggtgcgc 60gccattccca gagcagcgcc ccacctatat cgccacctcg atg cag gat ctc tac 115
Met Gln Asp Leu Tyr
1 5agc gat ccg ggc gag ctc aag cca ggt gcc cag ggc ggt ttt tca gcg 163Ser Asp Pro Gly Glu Leu Lys Pro Gly Ala Gln Gly Gly Phe Ser Ala
10 15 20ctt atc gac ggc gac acc ctg gtc att tcc ggc ggc gat gcc ggc gca 211Leu Ile Asp Gly Asp Thr Leu Val Ile Ser Gly Gly Asp Ala Gly Ala
25 30 35act ccg gtt gca gca ctc cgc act gcg ttg gat gtg gcc tgg gcg gcc 259Thr Pro Val Ala Ala Leu Arg Thr Ala Leu Asp Val Ala Trp Ala Ala
40 45 50aca gag cag tca ccg agg tac gcg ctg att cag agg tagctgctac 305Thr Glu Gln Ser Pro Arg Tyr Ala Leu Ile Gln Arg
55 60 65tgcattgcag agc 318<210>382<211>65<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>382Met Gln Asp Leu Tyr Ser Asp Pro Gly Glu Leu Lys Pro Gly Ala Gln1 5 10 15Gly Gly Phe Ser Ala Leu Ile Asp Gly Asp Thr Leu Val Ile Ser Gly
20 25 30Gly Asp Ala Gly Ala Thr Pro Val Ala Ala Leu Arg Thr Ala Leu Asp
35 40 45Val Ala Trp Ala Ala Thr Glu Gln Ser Pro Arg Tyr Ala Leu Ile Gln
50 55 60Arg65<210>383<211>1017<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(994)<223>RXN00393<400>383tctattcatt tcacaatagc gtttcacact cccccatagc ctgccgaacg tatttcaagc 60aattgcgcga tcgagtatgt gatggggaaa gatagaggtt atg tct cac acg gaa 115
Met Ser His Thr Glu
1 5ccc cag ccg aat tct gta act ttg tcc gat tgg att caa ggc gca cgc 163Pro Gln Pro Asn Ser Val Thr Leu Ser Asp Trp Ile Gln Gly Ala Arg
10 15 20ccg cgt acc tgg gca aat gcg ttc gcg cct gtc att gcc ggt tca ggt 211Pro Arg Thr Trp Ala Asn Ala Phe Ala Pro Val Ile Ala Gly Ser Gly
25 30 35gtc gcc gct ttt cat gat ggt ttt gtg tgg tgg aag gcc ttg ctg gcg 259Val Ala Ala Phe His Asp Gly Phe Val Trp Trp Lys Ala Leu Leu Ala
40 45 50ctt gtc gtg gcg tgg gct ttg atc atc ggt gtg aat tac gcc aat gat 307Leu Val Val Ala Trp Ala Leu Ile Ile Gly Val Asn Tyr Ala Asn Asp
55 60 65tac tct gat ggc att cgt ggc acc gat gaa gac cgc acc ggt cct ctg 355Tyr Ser Asp Gly Ile Arg Gly Thr Asp Glu Asp Arg Thr Gly Pro Leu70 75 80 85cga ctc act ggt tct ggg ttg gct gag ccg aag aaa gtg aaa gct gcg 403Arg Leu Thr Gly Ser Gly Leu Ala Glu Pro Lys Lys Val Lys Ala Ala
90 95 100gcg ttt att tct ttc ggt atc gca ggt gtc gcc ggc acc gcg ctg agc 451Ala Phe Ile Ser Phe Gly Ile Ala Gly Val Ala Gly Thr Ala Leu Ser
105 110 115ctg ttg agc gcg tgg tgg ctg atc ctc atc ggc atc ctg tgt gtg ctg 499Leu Leu Ser Ala Trp Trp Leu Ile Leu Ile Gly Ile Leu Cys Val Leu
120 125 130ggc gcg tgg ttc tac acc ggc ggt aaa aat cct tat ggt tac cgc ggg 547Gly Ala Trp Phe Tyr Thr Gly Gly Lys Asn Pro Tyr Gly Tyr Arg Gly
135 140 145ctc ggc gag att gct gtg ttc atc ttc ttc ggc ctc gtc gcg gtc atg 595Leu Gly Glu Ile Ala Val Phe Ile Phe Phe Gly Leu Val Ala Val Met150 155 160 165gga acg cag ttc acc caa acc ggt tcc gtc agc tgg gcc ggt ttg gcc 643Gly Thr Gln Phe Thr Gln Thr Gly Ser Val Ser Trp Ala Gly Leu Ala
170 175 180gcc gca gtt ggc gtg ggg tcg atg tct gct ggc gtg aac ttg gcc aac 691Ala Ala Val Gly Val Gly Ser Met Ser Ala Gly Val Asn Leu Ala Asn
185 190 195aat att cgc gat att cca acc gat agc aag acc gga aaa att acc ctc 739Asn Ile Arg Asp Ile Pro Thr Asp Ser Lys Thr Gly Lys Ile Thr Leu
200 205 210gcg gtc cgc ctg ggc gat gcg ggt gct cgt aag ctg ttc ctc gcg ctg 787Ala Val Arg Leu Gly Asp Ala Gly Ala Arg Lys Leu Phe Leu Ala Leu
215 220 225att tcc acg ccg ttc atc atg tcc atc tgc ctg gcg ttt gtc gcc tgg 835Ile Ser Thr Pro Phe Ile Met Ser Ile Cys Leu Ala Phe Val Ala Trp230 235 240 245cca gcg ctg atc gcg atc atc gtt ttc ccg ctg gca ctg aaa gcc gca 883Pro Ala Leu Ile Ala Ile Ile Val Phe Pro Leu Ala Leu Lys Ala Ala
250 255 260ggg ccg atc cgc aac aac gcc acc ggc aag gat ctc atc ccc gtc atc 931Gly Pro Ile Arg Asn Asn Ala Thr Gly Lys Asp Leu Ile Pro Val Ile
265 270 275ggc tca aca ggg cgc gcc atg gcg ttg tgg gcc gtg ctc acg ggc ctg 979Gly Ser Thr Gly Arg Ala Met Ala Leu Trp Ala Val Leu Thr Gly Leu
280 285 290gca tta gcg ttt agc taaaacgctt ttcgacgctc ccc 1017Ala Leu Ala Phe Ser
295<210>384<211>298<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>384Met Ser His Thr Glu Pro Gln Pro Asn Ser Val Thr Leu Ser Asp Trp1 5 10 15Ile Gln Gly Ala Arg Pro Arg Thr Trp Ala Asn Ala Phe Ala Pro Val
20 25 30Ile Ala Gly Ser Gly Val Ala Ala Phe His Asp Gly Phe Val Trp Trp
35 40 45Lys Ala Leu Leu Ala Leu Val Val Ala Trp Ala Leu Ile Ile Gly Val
50 55 60Asn Tyr Ala Asn Asp Tyr Ser Asp Gly Ile Arg Gly Thr Asp Glu Asp65 70 75 80Arg Thr Gly Pro Leu Arg Leu Thr Gly Ser Gly Leu Ala Glu Pro Lys
85 90 95Lys Val Lys Ala Ala Ala Phe Ile Ser Phe Gly Ile Ala Gly Val Ala
100 105 110Gly Thr Ala Leu Ser Leu Leu Ser Ala Trp Trp Leu Ile Leu Ile Gly
115 120 125Ile Leu Cys Val Leu Gly Ala Trp Phe Tyr Thr Gly Gly Lys Asn Pro
130 135 140Tyr Gly Tyr Arg Gly Leu Gly Glu Ile Ala Val Phe Ile Phe Phe Gly145 150 155 160Leu Val Ala Val Met Gly Thr Gln Phe Thr Gln Thr Gly Ser Val Ser
165 170 175Trp Ala Gly Leu Ala Ala Ala Val Gly Val Gly Ser Met Ser Ala Gly
180 185 190Val Asn Leu Ala Asn Asn Ile Arg Asp Ile Pro Thr Asp Ser Lys Thr
195 200 205Gly Lys Ile Thr Leu Ala Val Arg Leu Gly Asp Ala Gly Ala Arg Lys
210 215 220Leu Phe Leu Ala Leu Ile Ser Thr Pro Phe Ile Met Ser Ile Cys Leu225 230 235 240Ala Phe Val Ala Trp Pro Ala Leu Ile Ala Ile Ile Val Phe Pro Leu
245 250 255Ala Leu Lys Ala Ala Gly Pro Ile Arg Asn Asn Ala Thr Gly Lys Asp
260 265 270Leu Ile Pro Val Ile Gly Ser Thr Gly Arg Ala Met Ala Leu Trp Ala
275 280 285Val Leu Thr Gly Leu Ala Leu Ala Phe Ser
290 295<210>385<211>1242<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1219)<223>RXN00948<400>385acaccctcca aatgatctcg taaaacagta ttgaatttag gtacgactct aatcgtacct 60tgccctcaag ccaagctagt tgtacga rca aactcgttgt atg gca aac gtc gta 115
Met Ala Asn Val Val
1 5cta gtc gat cga atg gag cct ttg gtg tcc aag ctg ttt acc cca att 163Leu Val Asp Arg Met Glu Pro Leu Val Ser Lys Leu Phe Thr Pro Ile
10 15 20caa atc cgc gac atc acc atc ccc aac cgc gtg tgg atg tca ccg atg 211Gln Ile Arg Asp Ile Thr Ile Pro Asn Arg Val Trp Met Ser Pro Met
25 30 35tgc acc tac tct gca gcc acc ggt tca ggt ctt ccc acc gat ttt cac 259Cys Thr Tyr Ser Ala Ala Thr Gly Ser Gly Leu Pro Thr Asp Phe His
40 45 50cag gct cat tac gca gct cgc gca gca ggt ggt gtc gga tta gtc atg 307Gln Ala His Tyr Ala Ala Arg Ala Ala Gly Gly Val Gly Leu Val Met
55 60 65gtt gaa gca act gga gtg aac ccc gta gct ccc atc tcc cca gtc gac 355Val Glu Ala Thr Gly Val Asn Pro Val Ala Pro Ile Ser Pro Val Asp70 75 80 85ctt gga ctt tgg agc cat gac caa att gaa cca ttc tcc cga gtg aca 403Leu Gly Leu Trp Ser His Asp Gln Ile Glu Pro Phe Ser Arg Val Thr
90 95 100gca gct att cgc gcc ggt ggg gca gta ccg gcc gtt caa tta gcc cat 451Ala Ala Ile Arg Ala Gly Gly Ala Val Pro Ala Val Gln Leu Ala His
105 110 115gct ggc cgc aag gca tcc acc gat gct ccg tgg aat ggt ggc gga tat 499Ala Gly Arg Lys Ala Ser Thr Asp Ala Pro Trp Asn Gly Gly Gly Tyr
120 125 130gtt gga cca gaa acc aat gga tgg gag act gtc ggc ccc agc cct ctg 547Val Gly Pro Glu Thr Asn Gly Trp Glu Thr Val Gly Pro Ser Pro Leu
135 140 145gca ttc cca ggt ttg cct gct ccg cgc gag ctg acg gtt tca gaa atc 595Ala Phe Pro Gly Leu Pro Ala Pro Arg Glu Leu Thr Val Ser Glu Ile150 155 160 165caa gag gtt gtg cag cag ttc gct ggc gcc gcc gtt cgt gcc gat cag 643Gln Glu Val Val Gln Gln Phe Ala Gly Ala Ala Val Arg Ala Asp Gln
170 175 180gct ggt ttt gat gtc gtg gaa att cac gca gca cac ggc tac ctt ttg 691Ala Gly Phe Asp Val Val Glu Ile His Ala Ala His Gly Tyr Leu Leu
185 190 195cat aac ttc ctt tct ccg atc tcc aac aag cgc acc gat tca tac ggc 739His Asn Phe Leu Ser Pro Ile Ser Asn Lys Arg Thr Asp Ser Tyr Gly
200 205 210gga tct tta gaa aac cgc gct cgc atc gtg ctc gaa gtc att gat gca 787Gly Ser Leu Glu Asn Arg Ala Arg Ile Val Leu Glu Val Ile Asp Ala
215 220 225atc cgc gca gtg tgg cca gag gaa aag cct gta ttc atg cgc att tcc 835Ile Arg Ala Val Trp Pro Glu Glu Lys Pro Val Phe Met Arg Ile Ser230 235 240 245acc acc gac tgg gtg gag gaa aac cca cag gat gat cgc gag tcc tgg 883Thr Thr Asp Trp Val Glu Glu Asn Pro Gln Asp Asp Arg Glu Ser Trp
250 255 260acg ctg agc caa agc agg cag ctg gct ttg tgg gca tcc gag cac gga 931Thr Leu Ser Gln Ser Arg Gln Leu Ala Leu Trp Ala Ser Glu His Gly
265 270 275gtt gat ttg atc gat gcc tct tct ggt ggc ctc gac atc gtc ccc att 979Val Asp Leu Ile Asp Ala Ser Ser Gly Gly Leu Asp Ile Val Pro Ile
280 285 290ccg cat gac cgc gat tac caa acc gcg aag gcc gca gat ctt cac gca 1027Pro His Asp Arg Asp Tyr Gln Thr Ala Lys Ala Ala Asp Leu His Ala
295 300 305agt acc gga gtg aca gtc gct gct gtg ggg cgc att gat gac gcc caa 1075Ser Thr Gly Val Thr Val Ala Ala Val Gly Arg Ile Asp Asp Ala Gln310 315 320 325act gcg cac aat ttg gtt gat tct ggc gat gtc aat gca gtt ttc ctc 1123Thr Ala His Asn Leu Val Asp Ser Gly Asp Val Asn Ala Val Phe Leu
330 335 340ggc cgt cca ctg ctc aag gat cct tcc tgg gca aac caa gca gcc ctc 1171Gly Arg Pro Leu Leu Lys Asp Pro Ser Trp Ala Asn Gln Ala Ala Leu
345 350 355gca cta ggt gcg gaa ccc agg tat gtt cac caa tac gac tac gta ctt 1219Ala Leu Gly Ala Glu Pro Arg Tyr Val His Gln Tyr Asp Tyr Val Leu
360 365 370taaaggagag ttgacatgaa ggt 1242<210>386<211>373<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>386Met Ala Asn Val Val Leu Val Asp Arg Met Glu Pro Leu Val Ser Lys1 5 10 15Leu Phe Thr Pro Ile Gln Ile Arg Asp Ile Thr Ile Pro Asn Arg Val
20 25 30Trp Met Ser Pro Met Cys Thr Tyr Ser Ala Ala Thr Gly Ser Gly Leu
35 40 45Pro Thr Asp Phe His Gln Ala His Tyr Ala Ala Arg Ala Ala Gly Gly
50 55 60Val Gly Leu Val Met Val Glu Ala Thr Gly Val Asn Pro Val Ala Pro65 70 75 80Ile Ser Pro Val Asp Leu Gly Leu Trp Ser His Asp Gln Ile Glu Pro
85 90 95Phe Ser Arg Val Thr Ala Ala Ile Arg Ala Gly Gly Ala Val Pro Ala
100 105 110Val Gln Leu Ala His Ala Gly Arg Lys Ala Ser Thr Asp Ala Pro Trp
115 120 125Asn Gly Gly Gly Tyr Val Gly Pro Glu Thr Asn Gly Trp Glu Thr Val
130 135 140Gly Pro Ser Pro Leu Ala Phe Pro Gly Leu Pro Ala Pro Arg Glu Leu145 150 155 160Thr Val Ser Glu Ile Gln Glu Val Val Gln Gln Phe Ala Gly Ala Ala
165 170 175Val Arg Ala Asp Gln Ala Gly Phe Asp Val Val Glu Ile His Ala Ala
180 185 190His Gly Tyr Leu Leu His Asn Phe Leu Ser Pro Ile Ser Asn Lys Arg
195 200 205Thr Asp Ser Tyr Gly Gly Ser Leu Glu Asn Arg Ala Arg Ile Val Leu
210 215 220Glu Val Ile Asp Ala Ile Arg Ala Val Trp Pro Glu Glu Lys Pro Val225 230 235 240Phe Met Arg Ile Ser Thr Thr Asp Trp Val Glu Glu Asn Pro Gln Asp
245 250 255Asp Arg Glu Ser Trp Thr Leu Ser Gln Ser Arg Gln Leu Ala Leu Trp
260 265 270Ala Ser Glu His Gly Val Asp Leu Ile Asp Ala Ser Ser Gly Gly Leu
275 280 285Asp Ile Val Pro Ile Pro His Asp Arg Asp Tyr Gln Thr Ala Lys Ala
290 295 300Ala Asp Leu His Ala Ser Thr Gly Val Thr Val Ala Ala Val Gly Arg305 310 315 320Ile Asp Asp Ala Gln Thr Ala His Asn Leu Val Asp Ser Gly Asp Val
325 330 335Asn Ala Val Phe Leu Gly Arg Pro Leu Leu Lys Asp Pro Ser Trp Ala
340 345 350Asn Gln Ala Ala Leu Ala Leu Gly Ala Glu Pro Arg Tyr Val His Gln
355 360 365Tyr Asp Tyr Val Leu
370<210>387<211>873<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(850)<223>RXN01923<400>387ccaaagtgaa taccccgact gcagcagcgc aaaagttcaa gtactttggg atgcaaatct 60agtagcacgt cccatgtttc tcacactctc aggagctgac atg tct gca ctt att 115
Met Ser Ala Leu Ile
1 5aaa ggt tca gga cct cat cat gtg gtt gtc tta aat ggt tgg ttt ggt 163Lys Gly Ser Gly Pro His His Val Val Val Leu Asn Gly Trp Phe Gly
10 15 20cat gct gcg ggc tgg gga gct ttc gct gac tat ctt gac ctc ggc aac 211His Ala Ala Gly Trp Gly Ala Phe Ala Asp Tyr Leu Asp Leu Gly Asn
25 30 35tac acc tgg cac ttt tgg gat tac cga ggt tac ggc aac aga aaa gac 259Tyr Thr Trp His Phe Trp Asp Tyr Arg Gly Tyr Gly Asn Arg Lys Asp
40 45 50gac gca gga gaa ttt act ctg gag gaa att tca gcg gat atc gtt gca 307Asp Ala Gly Glu Phe Thr Leu Glu Glu Ile Ser Ala Asp Ile Val Ala
55 60 65tac atc gac tcg att gag gca gaa aag gtt tcc atc ctg ggc cat tcc 355Tyr Ile Asp Ser Ile Glu Ala Glu Lys Val Ser Ile Leu Gly His Ser70 75 80 85atg ggt gga gtg ttc atg cag aaa gtc ctt gca gac agc gcc acc ccc 403Met Gly Gly Val Phe Met Gln Lys Val Leu Ala Asp Ser Ala Thr Pro
90 95 100atc gct tca ctg gtt gga att tct gcc gtt gct gca gct gga aca cca 451Ile Ala Ser Leu Val Gly Ile Ser Ala Val Ala Ala Ala Gly Thr Pro
105 110 115ttc gat gag gat tct cgg aag ctt ttc acc tca gca ggg cac aac ccg 499Phe Asp Glu Asp Ser Arg Lys Leu Phe Thr Ser Ala Gly His Asn Pro
120 125 130gac tcg agg cga gcc atc atc gat ttc acc tca gga tct cgc caa cct 547Asp Ser Arg Arg Ala Ile Ile Asp Phe Thr Ser Gly Ser Arg Gln Pro
135 140 145gcc gcg tgg ttg gat gat ctc acc gac tcg gcg gtg cag aat tcc act 595Ala Ala Trp Leu Asp Asp Leu Thr Asp Ser Ala Val Gln Asn Ser Thr150 155 160 165cca gag gcc gtt gaa aag tac ttt ttt gcg tgg gct gat tgt aat ttc 643Pro Glu Ala Val Glu Lys Tyr Phe Phe Ala Trp Ala Asp Cys Asn Phe
170 175 180gca gcg gat tta ggc acc caa gat ttg ccc gtg gac att ctc acc ggc 691Ala Ala Asp Leu Gly Thr Gln Asp Leu Pro Val Asp Ile Leu Thr Gly
185 190 195gat ctc gac ccc gcg gtc act aaa act gcc gtg gaa tcc gca ttc ggc 739Asp Leu Asp Pro Ala Val Thr Lys Thr Ala Val Glu Ser Ala Phe Gly
200 205 210ccg atc tat caa aat ctg acc gtt gaa gaa ctc cac gat gtc gga cac 787Pro Ile Tyr Gln Asn Leu Thr Val Glu Glu Leu His Asp Val Gly His
215 220 225tac gca att ttc gag cac ccc tta ggc ctt gcc gcc agg gtg ctt cga 835Tyr Ala Ile Phe Glu His Pro Leu Gly Leu Ala Ala Arg Val Leu Arg230 235 240 245ttt ctc gac gcc gtc tagtacttcc gcaaattcac cgg 873Phe Leu Asp Ala Val
250<210>388<211>250<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>388Met Ser Ala Leu Ile Lys Gly Ser Gly Pro His His Val Val Val Leu1 5 10 15Asn Gly Trp Phe Gly His Ala Ala Gly Trp Gly Ala Phe Ala Asp Tyr
20 25 30Leu Asp Leu Gly Asn Tyr Thr Trp His Phe Trp Asp Tyr Arg Gly Tyr
35 40 45Gly Asn Arg Lys Asp Asp Ala Gly Glu Phe Thr Leu Glu Glu Ile Ser
50 55 60Ala Asp Ile Val Ala Tyr Ile Asp Ser Ile Glu Ala Glu Lys Val Ser65 70 75 80Ile Leu Gly His Ser Met Gly Gly Val Phe Met Gln Lys Val Leu Ala
85 90 95Asp Ser Ala Thr Pro Ile Ala Ser Leu Val Gly Ile Ser Ala Val Ala
100 105 110Ala Ala Gly Thr Pro Phe Asp Glu Asp Ser Arg Lys Leu Phe Thr Ser
115 120 125Ala Gly His Asn Pro Asp Ser Arg Arg Ala Ile Ile Asp Phe Thr Ser
130 135 140Gly Ser Arg Gln Pro Ala Ala Trp Leu Asp Asp Leu Thr Asp Ser Ala145 150 155 160Val Gln Asn Ser Thr Pro Glu Ala Val Glu Lys Tyr Phe Phe Ala Trp
165 170 175Ala Asp Cys Asn Phe Ala Ala Asp Leu Gly Thr Gln Asp Leu Pro Val
180 185 190Asp Ile Leu Thr Gly Asp Leu Asp Pro Ala Val Thr Lys Thr Ala Val
195 200 205Glu Ser Ala Phe Gly Pro Ile Tyr Gln Asn Leu Thr Val Glu Glu Leu
210 215 220His Asp Val Gly His Tyr Ala Ile Phe Glu His Pro Leu Gly Leu Ala225 230 235 240Ala Arg Val Leu Arg Phe Leu Asp Ala Val
245 250<210>389<211>873<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(850)<223>RXN00398<400>389tgagttcgcc accatcagca ccggcaccca ccagcgcggt gtggttaacc gtgagaagtt 60tgtctcccgt ctgcctgaag cacctaagga aaactaaatc atg gcc aag ttg ttt 115
Met Ala Lys Leu Phe
1 5gat tcc cat ttc cat atc atc gat ccc cag cac cca ctg atc gaa aac 163Asp Ser His Phe His Ile Ile Asp Pro Gln His Pro Leu Ile Glu Asn
10 15 20aac ggc tac ctc ccc gag cct ttc acc gtg gag gat tac act gcg cgt 211Asn Gly Tyr Leu Pro Glu Pro Phe Thr Val Glu Asp Tyr Thr Ala Arg
25 30 35gtt gaa ggc ctc gaa gtt gct gcc gga gcg att gtt tcc ggt tct ttc 259Val Glu Gly Leu Glu Val Ala Ala Gly Ala Ile Val Ser Gly Ser Phe
40 45 50cag gct ttc gac cag ggc tac ctc aaa gat gct ctc gca gtg ctt ggc 307Gln Ala Phe Asp Gln Gly Tyr Leu Lys Asp Ala Leu Ala Val Leu Gly
55 60 65cca ggc tat gtc ggt gtc act cag atc ccc gca gat acc tct gat cag 355Pro Gly Tyr Val Gly Val Thr Gln Ile Pro Ala Asp Thr Ser Asp Gln70 75 80 85gag att ctt gat ctg gac aaa gct ggc gtg aag gct gtg cgt tta aac 403Glu Ile Leu Asp Leu Asp Lys Ala Gly Val Lys Ala Val Arg Leu Asn
90 95 100ttg aag cgc ggt ggt tcg gca ggt ctt gac gat ctc gag acc ttg gca 451Leu Lys Arg Gly Gly Ser Ala Gly Leu Asp Asp Leu Glu Thr Leu Ala
105 110 115cgc cga gtc cac gac cta gcc ggt tgg cac acc gaa ctc tat gtg gat 499Arg Arg Val His Asp Leu Ala Gly Trp His Thr Glu Leu Tyr Val Asp
120 125 130gct cgc gaa cta gac gag ttg gaa tca acc ttg gcc tcc ctc cct gct 547Ala Arg Glu Leu Asp Glu Leu Glu Ser Thr Leu Ala Ser Leu Pro Ala
135 140 145gtc agc att gat cac tta ggg ctc cac cgc gat gga ctt ccc gca ctt 595Val Ser Ile Asp His Leu Gly Leu His Arg Asp Gly Leu Pro Ala Leu150 155 160 165ctt cgc ttg gta gaa aat ggc att aaa gtc aaa gca acc gga ttc gga 643Leu Arg Leu Val Glu Asn Gly Ile Lys Val Lys Ala Thr Gly Phe Gly
170 175 180cgg gta gaa cta gat cca act gaa gtc atc cag gca atc atg gct gtc 691Arg Val Glu Leu Asp Pro Thr Glu Val Ile Gln Ala Ile Met Ala Val
185 190 195gat ccc act gct ttg atg atc gga act gat ctt cca tcc acc cgc act 739Asp Pro Thr Ala Leu Met Ile Gly Thr Asp Leu Pro Ser Thr Arg Thr
200 205 210aag cga cct ttc gaa gac gct gac cta gat ttg atc gct gaa acg gtt 787Lys Arg Pro Phe Glu Asp Ala Asp Leu Asp Leu Ile Ala Glu Thr Val
215 220 225ggc gaa gat cat gtc gac aac gtc ttc tgg aac aac gct gca gcg ttc 835Gly Glu Asp His Val Asp Asn Val Phe Trp Asn Asn Ala Ala Ala Phe230 235 240 245tac ctc gga gac cag tagttttaag acccgaaatg tct 873Tyr Leu Gly Asp Gln
250<210>390<211>250<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>390Met Ala Lys Leu Phe Asp Ser His Phe His Ile Ile Asp Pro Gln His1 5 10 15Pro Leu Ile Glu Asn Asn Gly Tyr Leu Pro Glu Pro Phe Thr Val Glu
20 25 30Asp Tyr Thr Ala Arg Val Glu Gly Leu Glu Val Ala Ala Gly Ala Ile
35 40 45Val Ser Gly Ser Phe Gln Ala Phe Asp Gln Gly Tyr Leu Lys Asp Ala
50 55 60Leu Ala Val Leu Gly Pro Gly Tyr Val Gly Val Thr Gln Ile Pro Ala65 70 75 80Asp Thr Ser Asp Gln Glu Ile Leu Asp Leu Asp Lys Ala Gly Val Lys
85 90 95Ala Val Arg Leu Asn Leu Lys Arg Gly Gly Ser Ala Gly Leu Asp Asp
100 105 110Leu Glu Thr Leu Ala Arg Arg Val His Asp Leu Ala Gly Trp His Thr
115 120 125Glu Leu Tyr Val Asp Ala Arg Glu Leu Asp Glu Leu Glu Ser Thr Leu
130 135 140Ala Ser Leu Pro Ala Val Ser Ile Asp His Leu Gly Leu His Arg Asp145 150 155 160Gly Leu Pro Ala Leu Leu Arg Leu Val Glu Asn Gly Ile Lys Val Lys
165 170 175Ala Thr Gly Phe Gly Arg Val Glu Leu Asp Pro Thr Glu Val Ile Gln
180 185 190Ala Ile Met Ala Val Asp Pro Thr Ala Leu Met Ile Gly Thr Asp Leu
195 200 205Pro Ser Thr Arg Thr Lys Arg Pro Phe Glu Asp Ala Asp Leu Asp Leu
210 215 220Ile Ala Glu Thr Val Gly Glu Asp His Val Asp Asn Val Phe Trp Asn225 230 235 240Asn Ala Ala Ala Phe Tyr Leu Gly Asp Gln
245 250<210>391<211>1108<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1099)<223>RXN02813<400>391ggactctaaa ttgacccgat ctttatactc cgaccttgct ggtagtggag aacacctcag 60cagcctttcc gacgagactt tcctaaagaa tcttcttgtc gtg gag gcc gct ttg 115
Val Glu Ala Ala Leu
1 5gcg gtt gca gct gcc ccc gag cac gca gca atg gcg aag gcc acc att 163Ala Val Ala Ala Ala Pro Glu His Ala Ala Met Ala Lys Ala Thr Ile
10 15 20gat tct tat cag ttg gat gtg gag gag ctt tcc cgt cgc gca gcc gag 211Asp Ser Tyr Gln Leu Asp Val Glu Glu Leu Ser Arg Arg Ala Ala Glu
25 30 35ggc ggt aat ccg ctc att ccg ctg gtc act gac ctc aag gcc atc aat 259Gly Gly Asn Pro Leu Ile Pro Leu Val Thr Asp Leu Lys Ala Ile Asn
40 45 50ccg gca ggc atc cac att ggc gca acg agc cag gac atc att gat tct 307Pro Ala Gly Ile His Ile Gly Ala Thr Ser Gln Asp Ile Ile Asp Ser
55 60 65gcg tta atg ctg tgc atg aag gaa ggg gtg ggg gag gtc gtc gac aag 355Ala Leu Met Leu Cys Met Lys Glu Gly Val Gly Glu Val Val Asp Lys70 75 80 85ctt aaa aag ctt gcg cga gat ttg gcc gag ctc acc gcg gag cat aaa 403Leu Lys Lys Leu Ala Arg Asp Leu Ala Glu Leu Thr Ala Glu His Lys
90 95 100gca acc ccg atc atg ggg cgc acg ttg ggg cag atc gcg acg ccg acg 451Ala Thr Pro Ile Met Gly Arg Thr Leu Gly Gln Ile Ala Thr Pro Thr
105 110 115acg ttc ggc gcg ctg acc ggc ggc tgg ctg gtt gcg gtg gac aat gcg 499Thr Phe Gly Ala Leu Thr Gly Gly Trp Leu Val Ala Val Asp Asn Ala
120 125 130gca cgc gcc ctg gag gcg ctg gag ttt ccg gtg tcg tat ggc ggt gcc 547Ala Arg Ala Leu Glu Ala Leu Glu Phe Pro Val Ser Tyr Gly Gly Ala
135 140 145agc gga aat atg acg gcg gtg cac ccg cgt ggc ttc gag att cag gcg 595Ser Gly Asn Met Thr Ala Val His Pro Arg Gly Phe Glu Ile Gln Ala150 155 160 165aag ctg gcc gag gag ttg ggc ctt ttt gat ccg cag tgg gtg tgg cat 643Lys Leu Ala Glu Glu Leu Gly Leu Phe Asp Pro Gln Trp Val Trp His
170 175 180tcc gat cgc acg ccg atc act gcg atc gcg tcg gcg ctg gca acg gcc 691Ser Asp Arg Thr Pro Ile Thr Ala Ile Ala Ser Ala Leu Ala Thr Ala
185 190 195gct ggt gtg gta cgc aaa att gct ggt gac gtg gtg ttt tac tca caa 739Ala Gly Val Val Arg Lys Ile Ala Gly Asp Val Val Phe Tyr Ser Gln
200 205 210acc gag gtc ggc gag ttg cgg gag aaa tcc ccc ggc ggc agc tcc gcg 787Thr Glu Val Gly Glu Leu Arg Glu Lys Ser Pro Gly Gly Ser Ser Ala
215 220 225atg ccc cac aaa gcc aat ccg gcc gct gcg att gcg tgc gac ggt tac 835Met Pro His Lys Ala Asn Pro Ala Ala Ala Ile Ala Cys Asp Gly Tyr230 235 240 245gcg cgc cgg gca cct ggc ctt ctt gca acg ctt ttc gac gcc ctc gac 883Ala Arg Arg Ala Pro Gly Leu Leu Ala Thr Leu Phe Asp Ala Leu Asp
250 255 260tgc cgt ttg cag cgc ggc acc ggc agc tgg cac gcg gag tgg gca acg 931Cys Arg Leu Gln Arg Gly Thr Gly Ser Trp His Ala Glu Trp Ala Thr
265 270 275ctg cgc gag ttg gct gct gtc act cac tca gca gtg agc agg gct gca 979Leu Arg Glu Leu Ala Ala Val Thr His Ser Ala Val Ser Arg Ala Ala
280 285 290acc agc atc gat ggc atc acc gtc aac gtt gat gtg atg gca agt cgc 1027Thr Ser Ile Asp Gly Ile Thr Val Asn Val Asp Val Met Ala Ser Arg
295 300 305gtc aat gga cca acc ggg cac gcc gaa gat ttg gcg gag cgg gca cta 1075Val Asn Gly Pro Thr Gly His Ala Glu Asp Leu Ala Glu Arg Ala Leu310 315 320 325gaa att tat gga aaa gga cgc agt taatggatc 1108Glu Ile Tyr Gly Lys Gly Arg Ser
330<210>392<211>333<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>392Val Glu Ala Ala Leu Ala Val Ala Ala Ala Pro Glu His Ala Ala Met1 5 10 15Ala Lys Ala Thr Ile Asp Ser Tyr Gln Leu Asp Val Glu Glu Leu Ser
20 25 30Arg Arg Ala Ala Glu Gly Gly Asn Pro Leu Ile Pro Leu Val Thr Asp
35 40 45Leu Lys Ala Ile Asn Pro Ala Gly Ile His Ile Gly Ala Thr Ser Gln
50 55 60Asp Ile Ile Asp Ser Ala Leu Met Leu Cys Met Lys Glu Gly Val Gly65 70 75 80Glu Val Val Asp Lys Leu Lys Lys Leu Ala Arg Asp Leu Ala Glu Leu
85 90 95Thr Ala Glu His Lys Ala Thr Pro Ile Met Gly Arg Thr Leu Gly Gln
100 105 110Ile Ala Thr Pro Thr Thr Phe Gly Ala Leu Thr Gly Gly Trp Leu Val
115 120 125Ala Val Asp Asn Ala Ala Arg Ala Leu Glu Ala Leu Glu Phe Pro Val
130 135 140Ser Tyr Gly Gly Ala Ser Gly Asn Met Thr Ala Val His Pro Arg Gly145 150 155 160Phe Glu Ile Gln Ala Lys Leu Ala Glu Glu Leu Gly Leu Phe Asp Pro
165 170 175Gln Trp Val Trp His Ser Asp Arg Thr Pro Ile Thr Ala Ile Ala Ser
180 185 190Ala Leu Ala Thr Ala Ala Gly Val Val Arg Lys Ile Ala Gly Asp Val
195 200 205Val Phe Tyr Ser Gln Thr Glu Val Gly Glu Leu Arg Glu Lys Ser Pro
210 215 220Gly Gly Ser Ser Ala Met Pro His Lys Ala Asn Pro Ala Ala Ala Ile225 230 235 240Ala Cys Asp Gly Tyr Ala Arg Arg Ala Pro Gly Leu Leu Ala Thr Leu
245 250 255Phe Asp Ala Leu Asp Cys Arg Leu Gln Arg Gly Thr Gly Ser Trp His
260 265 270Ala Glu Trp Ala Thr Leu Arg Glu Leu Ala Ala Val Thr His Ser Ala
275 280 285Val Ser Arg Ala Ala Thr Ser Ile Asp Gly Ile Thr Val Asn Val Asp
290 295 300Val Met Ala Ser Arg Val Asn Gly Pro Thr Gly His Ala Glu Asp Leu305 310 315 320Ala Glu Arg Ala Leu Glu Ile Tyr Gly Lys Gly Arg Ser
325 330<210>393<211>1218<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1195)<223>RXN00136<400>393cagtgttgca tcatctagaa atcgattaat taaaccgggc acctgattaa cattgggctg 60cccggtttct tcctattaca agcgaaaggc aacgtgcccc atg agc gca gtg cag 115
Met Ser Ala Val Gln
1 5att ttc aac acc gtc cac gtc aat gga tct tcc ccc tat gat gtc cac 163Ile Phe Asn Thr Val His Val Asn Gly Ser Ser Pro Tyr Asp Val His
10 15 20att ggt tcc ggc ctc aac gag ctc att gtt cag cgc gca gcg gaa tca 211Ile Gly Ser Gly Leu Asn Glu Leu Ile Val Gln Arg Ala Ala Glu Ser
25 30 35ggc gcg gag cag gta gcg att ttg cac cag ccc agc atg gat gac att 259Gly Ala Glu Gln Val Ala Ile Leu His Gln Pro Ser Met Asp Asp Ile
40 45 50gca tcc gag ttg gat gca gca cta gtc gct gct ggt ttg aag gtc ctg 307Ala Ser Glu Leu Asp Ala Ala Leu Val Ala Ala Gly Leu Lys Val Leu
55 60 65cac ctt aat gtt ccc gat gcg gaa aac ggc aag tcc ttg gaa gta gcg 355His Leu Asn Val Pro Asp Ala Glu Asn Gly Lys Ser Leu Glu Val Ala70 75 80 85ggg cag tgc tgg gat gaa ttg ggt ggc gca gca ttc ggc cgc cgc gat 403Gly Gln Cys Trp Asp Glu Leu Gly Gly Ala Ala Phe Gly Arg Arg Asp
90 95 100atc gtc atc gga ctt ggt ggc ggt gct gcc aca gat ctc gcg gga ttc 451Ile Val Ile Gly Leu Gly Gly Gly Ala Ala Thr Asp Leu Ala Gly Phe
105 110 115gtc gct gct gca tgg atg cgt ggc gtg cgc gtc att cag gtt cca acc 499Val Ala Ala Ala Trp Met Arg Gly Val Arg Val Ile Gln Val Pro Thr
120 125 130acc ttg ttg gcc atg gtg gac gct gcg gtg ggc ggc aag act ggc atc 547Thr Leu Leu Ala Met Val Asp Ala Ala Val Gly Gly Lys Thr Gly Ile
135 140 145aat acc gcc gca ggc aag aac ctt gtg ggc gcg ttc cac gag cct gac 595Asn Thr Ala Ala Gly Lys Asn Leu Val Gly Ala Phe His Glu Pro Asp150 155 160 165gca gta ttc att gac acc gat cgc cta gcc acc ctg cct gac gcg gaa 643Ala Val Phe Ile Asp Thr Asp Arg Leu Ala Thr Leu Pro Asp Ala Glu
170 175 180atc atc gcg gga tcc gcc gaa atc atc aaa act ggt ttc atc gcc gac 691Ile Ile Ala Gly Ser Ala Glu Ile Ile Lys Thr Gly Phe Ile Ala Asp
185 190 195cca gaa atc ctg cgc ctt tac gaa act gat ccc gca gcc tgc ctg aag 739Pro Glu Ile Leu Arg Leu Tyr Glu Thr Asp Pro Ala Ala Cys Leu Lys
200 205 210aaa gaa gtc gaa ggc tcc cac cta cct gaa ctg att tgg cgc tcc gtc 787Lys Glu Val Glu Gly Ser His Leu Pro Glu Leu Ile Trp Arg Ser Val
215 220 225acc gtc aag ggc tcc gtg gtc ggc caa gac ctc aaa gaa tct agc ctg 835Thr Val Lys Gly Ser Val Val Gly Gln Asp Leu Lys Glu Ser Ser Leu230 235 240 245cgc gaa atc ctc aac tac gga cac acc ttt gcc cac gcc gtc gaa ctc 883Arg Glu Ile Leu Asn Tyr Gly His Thr Phe Ala His Ala Val Glu Leu
250 255 260cgc gaa aac ttc cgc tgg cgc cac ggc aat gcc gtt gca gtg ggc atg 931Arg Glu Asn Phe Arg Trp Arg His Gly Asn Ala Val Ala Val Gly Met
265 270 275atg ttc atc gcc aac ctc tcc cac aag ctc ggg ctt atc gac gcg ccc 979Met Phe Ile Ala Asn Leu Ser His Lys Leu Gly Leu Ile Asp Ala Pro
280 285 290ctc ctc gag cgc cac cgc tca atc ctg gcg gcc atc ggt ctg ccc act 1027Leu Leu Glu Arg His Arg Ser Ile Leu Ala Ala Ile Gly Leu Pro Thr
295 300 305tcc tac gaa ggc gga gcc ttc gac gag ctt tac gac ggt atg acc cgc 1075Ser Tyr Glu Gly Gly Ala Phe Asp Glu Leu Tyr Asp Gly Met Thr Arg310 315 320 325gac aag aaa aac cgc gac ggc aac atc cgc ttc gtc gca ctg acc gcc 1123Asp Lys Lys Asn Arg Asp Gly Ash Ile Arg Phe Val Ala Leu Thr Ala
330 335 340gtg ggc gag gtt acc cgc att gag ggg ccc tca aaa caa gat tta cag 1171Val Gly Glu Val Thr Arg Ile Glu Gly Pro Ser Lys Gln Asp Leu Gln
345 350 355agt gct tat gag gca atc agc cac taagtgttga gtaatctact agt 1218Ser Ala Tyr Glu Ala Ile Ser His
360 365<210>394<211>365<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>394Met Ser Ala Val Gln Ile Phe Asn Thr Val His Val Asn Gly Ser Ser1 5 10 15Pro Tyr Asp Val His Ile Gly Ser Gly Leu Asn Glu Leu Ile Val Gln
20 25 30Arg Ala Ala Glu Ser Gly Ala Glu Gln Val Ala Ile Leu His Gln Pro
35 40 45Ser Met Asp Asp Ile Ala Ser Glu Leu Asp Ala Ala Leu Val Ala Ala
50 55 60Gly Leu Lys Val Leu His Leu Asn Val Pro Asp Ala Glu Asn Gly Lys65 70 75 80Ser Leu Glu Val Ala Gly Gln Cys Trp Asp Glu Leu Gly Gly Ala Ala
85 90 95Phe Gly Arg Arg Asp Ile Val Ile Gly Leu Gly Gly Gly Ala Ala Thr
100 105 110Asp Leu Ala Gly Phe Val Ala Ala Ala Trp Met Arg Gly Val Arg Val
115 120 125Ile Gln Val Pro Thr Thr Leu Leu Ala Met Val Asp Ala Ala Val Gly
130 135 140Gly Lys Thr Gly Ile Asn Thr Ala Ala Gly Lys Asn Leu Val Gly Ala145 150 155 160Phe His Glu Pro Asp Ala Val Phe Ile Asp Thr Asp Arg Leu Ala Thr
165 170 175Leu Pro Asp Ala Glu Ile Ile Ala Gly Ser Ala Glu Ile Ile Lys Thr
180 185 190Gly Phe Ile Ala Asp Pro Glu Ile Leu Arg Leu Tyr Glu Thr Asp Pro
195 200 205Ala Ala Cys Leu Lys Lys Glu Val Glu Gly Ser His Leu Pro Glu Leu
210 215 220Ile Trp Arg Ser Val Thr Val Lys Gly Ser Val Val Gly Gln Asp Leu225 230 235 240Lys Glu Ser Ser Leu Arg Glu Ile Leu Asn Tyr Gly His Thr Phe Ala
245 250 255His Ala Val Glu Leu Arg Glu Asn Phe Arg Trp Arg His Gly Asn Ala
260 265 270Val Ala Val Gly Met Met Phe Ile Ala Asn Leu Ser His Lys Leu Gly
275 280 285Leu Ile Asp Ala Pro Leu Leu Glu Arg His Arg Ser Ile Leu Ala Ala
290 295 300Ile Gly Leu Pro Thr Ser Tyr Glu Gly Gly Ala Phe Asp Glu Leu Tyr305 310 315 320Asp Gly Met Thr Arg Asp Lys Lys Asn Arg Asp Gly Asn Ile Arg Phe
325 330 335Val Ala Leu Thr Ala Val Gly Glu Val Thr Arg Ile Glu Gly Pro Ser
340 345 350Lys Gln Asp Leu Gln Ser Ala Tyr Glu Ala Ile Ser His
355 360 365<210>395<211>1977<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1954)<223>RXN02508<400>395tgcacactgc tggtggtgag gccgcagacc tggcagccgc aagcaaagcc tccgaggccc 60aactcgcggc tcagtaaaac caaaaggaat ctttgaccac atg cgt aca tcc att 115
Met Arg Thr Ser Ile
1 5gcc act gtt tgt ttg tcc gga act ctt gct gaa aag ctg cgc gca gct 163Ala Thr Val Cys Leu Ser Gly Thr Leu Ala Glu Lys Leu Arg Ala Ala
10 15 20gca gat gct gga ttt gat ggt gtg gaa atc ttc gag cag gac ttg gtg 211Ala Asp Ala Gly Phe Asp Gly Val Glu Ile Phe Glu Gln Asp Leu Val
25 30 35gtt tcc ccg cat tcg gca gag cag att cgt cag cgg gct cag gat ttg 259Val Ser Pro His Ser Ala Glu Gln Ile Arg Gln Arg Ala Gln Asp Leu
40 45 50gga tta acc ctg gat ctg ttc cag ccg ttt cga gat ttc gaa ggt gtg 307Gly Leu Thr Leu Asp Leu Phe Gln Pro Phe Arg Asp Phe Glu Gly Val
55 60 65gaa gaa gag cag ttt ctg aag aat ctg cac cgc ttg gaa gag aag ttc 355Glu Glu Glu Gln Phe Leu Lys Asn Leu His Arg Leu Glu Glu Lys Phe70 75 80 85aag ctg atg aac agg ctt ggc att gag atg atc ttg ttg tgt tcc aat 403Lys Leu Met Asn Arg Leu Gly Ile Glu Met Ile Leu Leu Cys Ser Asn
90 95 100gtg ggc acc gcg acc atc aat gat gat gac ctt ttc gtg gag cag ttg 451Val Gly Thr Ala Thr Ile Asn Asp Asp Asp Leu Phe Val Glu Gln Leu
105 110 115cat cgt gca gca gat ttg gct gag aag tac aac gtc aag att gct tat 499His Arg Ala Ala Asp Leu Ala Glu Lys Tyr Asn Val Lys Ile Ala Tyr
120 125 130gaa gcg ttg gcg tgg ggc aag ttt gtc aat gat ttt gag cat gcg cat 547Glu Ala Leu Ala Trp Gly Lys Phe Val Asn Asp Phe Glu His Ala His
135 140 145gca ctt gtg gag aag gtg aat cac aag gcg ctg gga acc tgc ttg gat 595Ala Leu Val Glu Lys Val Asn His Lys Ala Leu Gly Thr Cys Leu Asp150 155 160 165acg ttc cat att ctt tcc cgt ggt tgg gaa acc gac gag gtg gag aac 643Thr Phe His Ile Leu Ser Arg Gly Trp Glu Thr Asp Glu Val Glu Asn
170 175 180atc cct gcg gag aag atc ttc ttt gtt cag tta gcg gat gcg ccg aag 691Ile Pro Ala Glu Lys Ile Phe Phe Val Gln Leu Ala Asp Ala Pro Lys
185 190 195ctg agc atg gac att ttg tcc tgg tcg cgt cac cac cgt gtt ttc cct 739Leu Ser Met Asp Ile Leu Ser Trp Ser Arg His His Arg Val Phe Pro
200 205 210ggt gaa ggc gat ttc gat ctg gtg aaa ttc atg gtt cat ctg gcc aag 787Gly Glu Gly Asp Phe Asp Leu Val Lys Phe Met Val His Leu Ala Lys
215 220 225acg ggt tat gat ggc ccg att tct ttg gag atc ttc aac gat tcc ttc 835Thr Gly Tyr Asp Gly Pro Ile Ser Leu Glu Ile Phe Asn Asp Ser Phe230 235 240 245cgc aag gcc gag gtt ggt cgc acc gcg att gat ggg ttg cgt tct ttg 883Arg Lys Ala Glu Val Gly Arg Thr Ala Ile Asp Gly Leu Arg Ser Leu
250 255 260cgt tgg ttg gaa gat cag acc tgg cat gcg cta aat gct gag gat cgt 931Arg Trp Leu Glu Asp Gln Thr Trp His Ala Leu Asn Ala Glu Asp Arg
265 270 275cca agc gct ctt gaa ctg cgt gca ctt cct gag gtc gcg gaa cct gag 979Pro Ser Ala Leu Glu Leu Arg Ala Leu Pro Glu Val Ala Glu Pro Glu
280 285 290ggt gtt gat ttc att gag atc gcc act gga cgt ttg ggt gag acc att 1027Gly Val Asp Phe Ile Glu Ile Ala Thr Gly Arg Leu Gly Glu Thr Ile
295 300 305cgg gtt ctt cat caa ttg ggt ttc cgc ttg ggt ggt cat cac tgc agt 1075Arg Val Leu His Gln Leu Gly Phe Arg Leu Gly Gly His His Cys Ser310 315 320 325aag cag gat tac cag gta tgg acc cag ggc gat gtg cgc att gtg gtg 1123Lys Gln Asp Tyr Gln Val Trp Thr Gln Gly Asp Val Arg Ile Val Val
330 335 340tgt gat cgt ggg gtc acc ggg gct cca acc acg atc tct gcg atg ggc 1171Cys Asp Arg Gly Val Thr Gly Ala Pro Thr Thr Ile Ser Ala Met Gly
345 350 355ttt gac acc ccc gat cca gaa gct gct cat gcc cgt gcg gaa ttg ctg 1219Phe Asp Thr Pro Asp Pro Glu Ala Ala His Ala Arg Ala Glu Leu Leu
360 365 370cgg gct cag aca att gat cgt ccc cac atc gag ggc gaa gtt gac cta 1267Arg Ala Gln Thr Ile Asp Arg Pro His Ile Glu Gly Glu Val Asp Leu
375 380 385aaa ggt gtg tac gca ccg gat ggg gtg gag ctg ttt ttc gcg ggg ccg 1315Lys Gly Val Tyr Ala Pro Asp Gly Val Glu Leu Phe Phe Ala Gly Pro390 395 400 405agc ccc gat gga atg ccc gag tgg ctg ccg gaa ttc ggc gtc gaa aag 1363Ser Pro Asp Gly Met Pro Glu Trp Leu Pro Glu Phe Gly Val Glu Lys
410 415 420caa gaa gct ggt ctc att gaa gcc atc gac cac gtc aat ttc gcc cag 1411Gln Glu Ala Gly Leu Ile Glu Ala Ile Asp His Val Asn Phe Ala Gln
425 430 435ccg tgg caa cat ttt gat gag gca gtg ctg ttt tac acc gcg ctg atg 1459Pro Trp Gln His Phe Asp Glu Ala Val Leu Phe Tyr Thr Ala Leu Met
440 445 450gcg ttg gag act gtg cgt gag gat gag ttc ccg agc cca att ggt ttg 1507Ala Leu Glu Thr Val Arg Glu Asp Glu Phe Pro Ser Pro Ile Gly Leu
455 460 465gtg cgc aat cag gtg atg cgt tcg ccg aat gat gcg gtg cgg ttg ctg 1555Val Arg Asn Gln Val Met Arg Ser Pro Asn Asp Ala Val Arg Leu Leu470 475 480 485ctc agc gtg gcg ccc gag gac ggt gag cag gga gat ttc ctc aac gcg 1603Leu Ser Val Ala Pro Glu Asp Gly Glu Gln Gly Asp Phe Leu Asn Ala
490 495 500gcc tac ccg gag cac att gcg ttg gcc acg gcg gac atc gtg gcg gtg 1651Ala Tyr Pro Glu His Ile Ala Leu Ala Thr Ala Asp Ile Val Ala Val
505 510 515gct gaa cgt gcg cgc aaa cga ggc ctg gat ttc ttg ccc gtc cca gag 1699Ala Glu Arg Ala Arg Lys Arg Gly Leu Asp Phe Leu Pro Val Pro Glu
520 525 530aat tac tac gac gat gtg cag gcg cgt ttt gat ttg ccg cag gaa ttc 1747Asn Tyr Tyr Asp Asp Val Gln Ala Arg Phe Asp Leu Pro Gln Glu Phe
535 540 545ttg gac aca ctc aag gaa aac cac ctg ctt tac gac cgc gac gag aac 1795Leu Asp Thr Leu Lys Glu Asn His Leu Leu Tyr Asp Arg Asp Glu Asn550 555 560 565ggc gaa ttc ctc cac ttt tac acc cgc acg ttg ggc acg ctg ttc ttc 1843Gly Glu Phe Leu His Phe Tyr Thr Arg Thr Leu Gly Thr Leu Phe Phe
570 575 580gaa gtg gtg gaa cgc cgc ggc ggt ttt gca ggt tgg ggc gaa aca aac 1891Glu Val Val Glu Arg Arg Gly Gly Phe Ala Gly Trp Gly Glu Thr Asn
585 590 595gct ccg gtg cgg ttg gcg gcg cag tat cgt gag gtg cgg gac ctc gag 1939Ala Pro Val Arg Leu Ala Ala Gln Tyr Arg Glu Val Arg Asp Leu Glu
600 605 610cgg gga atc cca aac tagcatcccg aactagcccc cca 1977Arg Gly Ile Pro Asn
615<210>396<211>618<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>396Met Arg Thr Ser Ile Ala Thr Val Cys Leu Ser Gly Thr Leu Ala Glu1 5 10 15Lys Leu Arg Ala Ala Ala Asp Ala Gly Phe Asp Gly Val Glu Ile Phe
20 25 30Glu Gln Asp Leu Val Val Ser Pro His Ser Ala Glu Gln Ile Arg Gln
35 40 45Arg Ala Gln Asp Leu Gly Leu Thr Leu Asp Leu Phe Gln Pro Phe Arg
50 55 60Asp Phe Glu Gly Val Glu Glu Glu Gln Phe Leu Lys Asn Leu His Arg65 70 75 80Leu Glu Glu Lys Phe Lys Leu Met Asn Arg Leu Gly Ile Glu Met Ile
85 90 95Leu Leu Cys Ser Asn Val Gly Thr Ala Thr Ile Asn Asp Asp Asp Leu
100 105 110Phe Val Glu Gln Leu His Arg Ala Ala Asp Leu Ala Glu Lys Tyr Asn
115 120 125Val Lys Ile Ala Tyr Glu Ala Leu Ala Trp Gly Lys Phe Val Asn Asp
130 135 140Phe Glu His Ala His Ala Leu Val Glu Lys Val Asn His Lys Ala Leu145 150 155 160Gly Thr Cys Leu Asp Thr Phe His Ile Leu Ser Arg Gly Trp Glu Thr
165 170 175Asp Glu Val Glu Asn Ile Pro Ala Glu Lys Ile Phe Phe Val Gln Leu
180 185 190Ala Asp Ala Pro Lys Leu Ser Met Asp Ile Leu Ser Trp Ser Arg His
195 200 205His Arg Val Phe Pro Gly Glu Gly Asp Phe Asp Leu Val Lys Phe Met
210 215 220Val His Leu Ala Lys Thr Gly Tyr Asp Gly Pro Ile Ser Leu Glu Ile225 230 235 240Phe Asn Asp Ser Phe Arg Lys Ala Glu Val Gly Arg Thr Ala Ile Asp
245 250 255Gly Leu Arg Ser Leu Arg Trp Leu Glu Asp Gln Thr Trp His Ala Leu
260 265 270Asn Ala Glu Asp Arg Pro Ser Ala Leu Glu Leu Arg Ala Leu Pro Glu
275 280 285Val Ala Glu Pro Glu Gly Val Asp Phe Ile Glu Ile Ala Thr Gly Arg
290 295 300Leu Gly Glu Thr Ile Arg Val Leu His Gln Leu Gly Phe Arg Leu Gly305 310 315 320Gly His His Cys Ser Lys Gln Asp Tyr Gln Val Trp Thr Gln Gly Asp
325 330 335Val Arg Ile Val Val Cys Asp Arg Gly Val Thr Gly Ala Pro Thr Thr
340 345 350Ile Ser Ala Met Gly Phe Asp Thr Pro Asp Pro Glu Ala Ala His Ala
355 360 365Arg Ala Glu Leu Leu Arg Ala Gln Thr Ile Asp Arg Pro His Ile Glu
370 375 380Gly Glu Val Asp Leu Lys Gly Val Tyr Ala Pro Asp Gly Val Glu Leu385 390 395 400Phe Phe Ala Gly Pro Ser Pro Asp Gly Met Pro Glu Trp Leu Pro Glu
405 410 415Phe Gly Val Glu Lys Gln Glu Ala Gly Leu Ile Glu Ala Ile Asp His
420 425 430Val Asn Phe Ala Gln Pro Trp Gln His Phe Asp Glu Ala Val Leu Phe
435 440 445Tyr Thr Ala Leu Met Ala Leu Glu Thr Val Arg Glu Asp Glu Phe Pro
450 455 460Ser Pro Ile Gly Leu Val Arg Asn Gln Val Met Arg Ser Pro Asn Asp465 470 475 480Ala Val Arg Leu Leu Leu Ser Val Ala Pro Glu Asp Gly Glu Gln Gly
485 490 495Asp Phe Leu Asn Ala Ala Tyr Pro Glu His Ile Ala Leu Ala Thr Ala
500 505 510Asp Ile Val Ala Val Ala Glu Arg Ala Arg Lys Arg Gly Leu Asp Phe
515 520 525Leu Pro Val Pro Glu Asn Tyr Tyr Asp Asp Val Gln Ala Arg Phe Asp
530 535 540Leu Pro Gln Glu Phe Leu Asp Thr Leu Lys Glu Asn His Leu Leu Tyr545 550 555 560Asp Arg Asp Glu Asn Gly Glu Phe Leu His Phe Tyr Thr Arg Thr Leu
565 570 575Gly Thr Leu Phe Phe Glu Val Val Glu Arg Arg Gly Gly Phe Ala Gly
580 585 590Trp Gly Glu Thr Asn Ala Pro Val Arg Leu Ala Ala Gln Tyr Arg Glu
595 600 605Val Arg Asp Leu Glu Arg Gly Ile Pro Asn
610 615<210>397<211>470<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(447)<223>RXN02839<400>397tgt gtg gtg aat gat tat gct gac cgc aag ttt gat ggt cat gtt aag 48Cys Val Val Asn Asp Tyr Ala Asp Arg Lys Phe Asp Gly His Val Lys1 5 10 15cgc acg gcg aac cga cca ctt ccc agc ggc gcg gta aca gag aaa gag 96Arg Thr Ala Asn Arg Pro Leu Pro Ser Gly Ala Val Thr Glu Lys Glu
20 25 30gcg cgc gcg ctg ttt gtc gtg ctg gta ctg att tcg ttt tta ctg gtg 144Ala Arg Ala Leu Phe Val Val Leu Val Leu Ile Ser Phe Leu Leu Val
35 40 45ctg acg ctg aat acg atg acc att ctg ttg tcg att gcc gcg cta gcg 192Leu Thr Leu Asn Thr Met Thr Ile Leu Leu Ser Ile Ala Ala Leu Ala
50 55 60ctg gcg tgg gtg tac ccg ttt atg aag cgg tat acc cat cta ccg caa 240Leu Ala Trp Val Tyr Pro Phe Met Lys Arg Tyr Thr His Leu Pro Gln65 70 75 80gtg gtg ctg ggc gcg gcg ttt ggc tgg tcg att cca atg gct ttt gcc 288Val Val Leu Gly Ala Ala Phe Gly Trp Ser Ile Pro Met Ala Phe Ala
85 90 95gct gtg agt gag tcg gtg cca ttg agt tgc tgg tta atg ttc ctc gcc 336Ala Val Ser Glu Ser Val Pro Leu Ser Cys Trp Leu Met Phe Leu Ala
100 105 110aat att ctc tgg gcg gtg gct tac gac acg cag tat gcg atg gtt gac 384Asn Ile Leu Trp Ala Val Ala Tyr Asp Thr Gln Tyr Ala Met Val Asp
115 120 125cgc gat gat gat gtg aag att ggc att aaa tcc acg gca atc ctg ttg 432Arg Asp Asp Asp Val Lys Ile Gly Ile Lys Ser Thr Ala Ile Leu Leu
130 135 140gcc aat acg ata aat tgatattggg attttgcaga ttg 470Ala Asn Thr Ile Asn145<210>398<211>149<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>398Cys Val Val Asn Asp Tyr Ala Asp Arg Lys Phe Asp Gly His Val Lys1 5 10 15Arg Thr Ala Asn Arg Pro Leu Pro Ser Gly Ala Val Thr Glu Lys Glu
20 25 30Ala Arg Ala Leu Phe Val Val Leu Val Leu Ile Ser Phe Leu Leu Val
35 40 45Leu Thr Leu Asn Thr Met Thr Ile Leu Leu Ser Ile Ala Ala Leu Ala
50 55 60Leu Ala Trp Val Tyr Pro Phe Met Lys Arg Tyr Thr His Leu Pro Gln65 70 75 80Val Val Leu Gly Ala Ala Phe Gly Trp Ser Ile Pro Met Ala Phe Ala
85 90 95Ala Val Ser Glu Ser Val Pro Leu Ser Cys Trp Leu Met Phe Leu Ala
100 105 110Asn Ile Leu Trp Ala Val Ala Tyr Asp Thr Gln Tyr Ala Met Val Asp
115 120 125Arg Asp Asp Asp Val Lys Ile Gly Ile Lys Ser Thr Ala Ile Leu Leu
130 135 140Ala Asn Thr Ile Asn145<210>399<211>978<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(955)<223>RXN00639<400>399agtgttgtta tcgagttcag ccgatcacaa agatttttcc gctaggcagt gatccgactc 60gcacccccta cttcaccccc aaagtctcta ggagtatgac atg act tca gct gaa 115
Met Thr Ser Ala Glu
1 5cag atc gtt gat cca aca gcc cac gat tcg ggc aac aag gca act gac 163Gln Ile Val Asp Pro Thr Ala His Asp Ser Gly Asn Lys Ala Thr Asp
10 15 20aag ttc aag gca aac cgc gtt tcc tcc gat acc tcc aag gaa cgc gca 211Lys Phe Lys Ala Asn Arg Val Ser Ser Asp Thr Ser Lys Glu Arg Ala
25 30 35aac gcg atc tac gta gat ctg ctc gcg gcg atc gcc cag gtt gct cac 259Asn Ala Ile Tyr Val Asp Leu Leu Ala Ala Ile Ala Gln Val Ala His
40 45 50aag cac gaa gtc acc tac gaa gag tac gca gtg ctc aag cag tgg atg 307Lys His Glu Val Thr Tyr Glu Glu Tyr Ala Val Leu Lys Gln Trp Met
55 60 65atc gac gtt gga gaa tac ggc gag tgg cca ctg tgg ttg gac gtt ttc 355Ile Asp Val Gly Glu Tyr Gly Glu Trp Pro Leu Trp Leu Asp Val Phe70 75 80 85gtt gag cat gag atc gaa gag atc aac tac aac cgc cac gac tac acc 403Val Glu His Glu Ile Glu Glu Ile Asn Tyr Asn Arg His Asp Tyr Thr
90 95 100gga acc aag ggt tcc atc gaa ggc cct tat tac gta gag aac tct ccg 451Gly Thr Lys Gly Ser Ile Glu Gly Pro Tyr Tyr Val Glu Asn Ser Pro
105 110 115aag ctc cct tgg gat gct gaa atg cca atg cgt gac aag gac cgc gca 499Lys Leu Pro Trp Asp Ala Glu Met Pro Met Arg Asp Lys Asp Arg Ala
120 125 130tgc acc cca ctg atc ttc gag ggg cag gtt act gac ctc gac ggc aac 547Cys Thr Pro Leu Ile Phe Glu Gly Gln Val Thr Asp Leu Asp Gly Asn
135 140 145ggt ctt gat gga gca gaa gtt gag ctc tgg cac gca gat gag gac gga 595Gly Leu Asp Gly Ala Glu Val Glu Leu Trp His Ala Asp Glu Asp Gly150 155 160 165tac tac tcc cag ttc gcg cct gga atc cca gag tgg aac ctg cgt ggc 643Tyr Tyr Ser Gln Phe Ala Pro Gly Ile Pro Glu Trp Asn Leu Arg Gly
170 175 180acc atc gtt acc gat gag gaa ggc cgc tac aag atc aag acc ctg cag 691Thr Ile Val Thr Asp Glu Glu Gly Arg Tyr Lys Ile Lys Thr Leu Gln
185 190 195cct gcg cct tac cag atc cct cat gat ggc cca acc ggt tgg ttc att 739Pro Ala Pro Tyr Gln Ile Pro His Asp Gly Pro Thr Gly Trp Phe Ile
200 205 210gag tct tac ggt ggg cac cca tgg cgc cca gcc cac ctc cac ttg cgc 787Glu Ser Tyr Gly Gly His Pro Trp Arg Pro Ala His Leu His Leu Arg
215 220 225gtt tcc cac ccg ggc tac cgc acc atc acc acc cag ctt tac ttc gag 835Val Ser His Pro Gly Tyr Arg Thr Ile Thr Thr Gln Leu Tyr Phe Glu230 235 240 245ggt ggc gag tgg gtc gaa aac gac gtt gca acc gct gtg aag cca gaa 883Gly Gly Glu Trp Val Glu Asn Asp Val Ala Thr Ala Val Lys Pro Glu
250 255 260ctg gtc ctg cac cct gag act ggc gag gat ggt aac cac gtt cac tac 931Leu Val Leu His Pro Glu Thr Gly Glu Asp Gly Asn His Val His Tyr
265 270 275cca ttc gtc ctg gat aag gaa gac tagtttttct acctagctag cat 978Pro Phe Val Leu Asp Lys Glu Asp
280 285<210>400<211>285<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>400Met Thr Ser Ala Glu Gln Ile Val Asp Pro Thr Ala His Asp Ser Gly1 5 10 15Asn Lys Ala Thr Asp Lys Phe Lys Ala Asn Arg Val Ser Ser Asp Thr
20 25 30Ser Lys Glu Arg Ala Asn Ala Ile Tyr Val Asp Leu Leu Ala Ala Ile
35 40 45Ala Gln Val Ala His Lys His Glu Val Thr Tyr Glu Glu Tyr Ala Val
50 55 60Leu Lys Gln Trp Met Ile Asp Val Gly Glu Tyr Gly Glu Trp Pro Leu65 70 75 80Trp Leu Asp Val Phe Val Glu His Glu Ile Glu Glu Ile Asn Tyr Asn
85 90 95Arg His Asp Tyr Thr Gly Thr Lys Gly Ser Ile Glu Gly Pro Tyr Tyr
100 105 110Val Glu Asn Ser Pro Lys Leu Pro Trp Asp Ala Glu Met Pro Met Arg
115 120 125Asp Lys Asp Arg Ala Cys Thr Pro Leu Ile Phe Glu Gly Gln Val Thr
130 135 140Asp Leu Asp Gly Asn Gly Leu Asp Gly Ala Glu Val Glu Leu Trp His145 150 155 160Ala Asp Glu Asp Gly Tyr Tyr Ser Gln Phe Ala Pro Gly Ile Pro Glu
165 170 175Trp Asn Leu Arg Gly Thr Ile Val Thr Asp Glu Glu Gly Arg Tyr Lys
180 185 190Ile Lys Thr Leu Gln Pro Ala Pro Tyr Gln Ile Pro His Asp Gly Pro
195 200 205Thr Gly Trp Phe Ile Glu Ser Tyr Gly Gly His Pro Trp Arg Pro Ala
210 215 220His Leu His Leu Arg Val Ser His Pro Gly Tyr Arg Thr Ile Thr Thr225 230 235 240Gln Leu Tyr Phe Glu Gly Gly Glu Trp Val Glu Asn Asp Val Ala Thr
245 250 255Ala Val Lys Pro Glu Leu Val Leu His Pro Glu Thr Gly Glu Asp Gly
260 265 270Asn His Val His Tyr Pro Phe Val Leu Asp Lys Glu Asp
275 280 285<210>401<211>780<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(757)<223>RXN02530<400>401gaggctgagc tttccgtcaa acaacatctt cccaatgtgg atgaaatgac tgtgaccatc 60accccttcca aaccttgagt cccgtgatac aattgttgat atg tca aca aat tat 115
Met Ser Thr Asn Tyr
1 5gaa gca atc atc att gga gca ggt cag gct gga ctc gcg gcg gcg cat 163Glu Ala Ile Ile Ile Gly Ala Gly Gln Ala Gly Leu Ala Ala Ala His
10 15 20gaa ctt tcc cgc cgc ggt ttc act ccc gga aaa gat ttt ctc gtc ctc 211Glu Leu Ser Arg Arg Gly Phe Thr Pro Gly Lys Asp Phe Leu Val Leu
25 30 35gat tcc aac gac ggg ccc ggt ggc gcc tgg cgg cat agg tgg gat tca 259Asp Ser Asn Asp Gly Pro Gly Gly Ala Trp Arg His Arg Trp Asp Ser
40 45 50ctc aca tta ggt aaa gcc cac gga atc gcc gat ctc cca ggg ctt ccc 307Leu Thr Leu Gly Lys Ala His Gly Ile Ala Asp Leu Pro Gly Leu Pro
55 60 65atg aat cgc ccc gat ccg aaa act ccg gct tcc aca ttg gtt gct ggt 355Met Asn Arg Pro Asp Pro Lys Thr Pro Ala Ser Thr Leu Val Ala Gly70 75 80 85tat tac ggc gct tac gag aac gag ttc tcc ttc gca gtt gtg cgc cca 403Tyr Tyr Gly Ala Tyr Glu Asn Glu Phe Ser Phe Ala Val Val Arg Pro
90 95 100gtc aaa gtc tca cga gtt gag ccc act tcc gag gat cct tcg agc cca 451Val Lys Val Ser Arg Val Glu Pro Thr Ser Glu Asp Pro Ser Ser Pro
105 110 115ttg cgc gtg agc agc gac gat ggt cga gag tgg att acc cgc atg gtt 499Leu Arg Val Ser Ser Asp Asp Gly Arg Glu Trp Ile Thr Arg Met Val
120 125 130ctt aat gca aca ggt acg tgg aca aac cct tat gtt ccg tac att cct 547Leu Asn Ala Thr Gly Thr Trp Thr Asn Pro Tyr Val Pro Tyr Ile Pro
135 140 145ggc atc gat aaa ttc cag ggc aag cag ctc cac acc gtt aat tac cgc 595Gly Ile Asp Lys Phe Gln Gly Lys Gln Leu His Thr Val Asn Tyr Arg150 155 160 165aag gcc gag gat ttc aaa ggt aag aaa gtc ctg gtc gtc ggc ggt ggt 643Lys Ala Glu Asp Phe Lys Gly Lys Lys Val Leu Val Val Gly Gly Gly
170 175 180ttg agt gct gtg caa ttt ctg ctg gag ttg gaa ggc ttg gcg gaa acc 691Leu Ser Ala Val Gln Phe Leu Leu Glu Leu Glu Gly Leu Ala Glu Thr
185 190 195acc tgg gcg acg cgt cgt ccg cga act tac gca gcg cga gtt cga cgc 739Thr Trp Ala Thr Arg Arg Pro Arg Thr Tyr Ala Ala Arg Val Arg Arg
200 205 210cgg ctg ggg cat tgc ggt tgagcgcgcc gtccgcgaac gca 780Arg Leu Gly His Cys Gly
215<210>402<211>219<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>402Met Ser Thr Asn Tyr Glu Ala Ile Ile Ile Gly Ala Gly Gln Ala Gly1 5 10 15Leu Ala Ala Ala His Glu Leu Ser Arg Arg Gly Phe Thr Pro Gly Lys
20 25 30Asp Phe Leu Val Leu Asp Ser Asn Asp Gly Pro Gly Gly Ala Trp Arg
35 40 45His Arg Trp Asp Ser Leu Thr Leu Gly Lys Ala His Gly Ile Ala Asp
50 55 60Leu Pro Gly Leu Pro Met Asn Arg Pro Asp Pro Lys Thr Pro Ala Ser65 70 75 80Thr Leu Val Ala Gly Tyr Tyr Gly Ala Tyr Glu Asn Glu Phe Ser Phe
85 90 95Ala Val Val Arg Pro Val Lys Val Ser Arg Val Glu Pro Thr Ser Glu
100 105 110Asp Pro Ser Ser Pro Leu Arg Val Ser Ser Asp Asp Gly Arg Glu Trp
115 120 125Ile Thr Arg Met Val Leu Asn Ala Thr Gly Thr Trp Thr Asn Pro Tyr
130 135 140Val Pro Tyr Ile Pro Gly Ile Asp Lys Phe Gln Gly Lys Gln Leu His145 150 155 160Thr Val Asn Tyr Arg Lys Ala Glu Asp Phe Lys Gly Lys Lys Val Leu
165 170 175Val Val Gly Gly Gly Leu Ser Ala Val Gln Phe Leu Leu Glu Leu Glu
180 185 190Gly Leu Ala Glu Thr Thr Trp Ala Thr Arg Arg Pro Arg Thr Tyr Ala
195 200 205Ala Arg Val Arg Arg Arg Leu Gly His Cys Gly
210 215<210>403<211>990<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(967)<223>RXN00434<400>403ccacgcgcca aaccatgcaa gccatcgtcc aaactgagga gaaggtcact gcttctctgg 60aattacagga agtccccgtc ccgaccctga agccaggtag gtg ctg gtg aag gtg 115
Val Leu Val Lys Val
1 5aag cct gcg ggc gtt aac cgt gcg gat ctt ttg cag acg cag gga aat 163Lys Pro Ala Gly Val Asn Arg Ala Asp Leu Leu Gln Thr Gln Gly Asn
10 15 20tat cct gtg ccg gcg ggg gct tcg gag att ctc ggg ctg gaa tgt gcg 211Tyr Pro Val Pro Ala Gly Ala Ser Glu Ile Leu Gly Leu Glu Cys Ala
25 30 35ggt gtg atc gtg aat gcc ggc gac act ggg caa aca gtg ggt cag gaa 259Gly Val Ile Val Asn Ala Gly Asp Thr Gly Gln Thr Val Gly Gln Glu
40 45 50gtc gct tgc ctt ctc act ggc ggt gga tat gcg caa tat gtg gcg gtt 307Val Ala Cys Leu Leu Thr Gly Gly Gly Tyr Ala Gln Tyr Val Ala Val
55 60 65ccg gaa ggt cag ttg atg cca att cca gag ggt tac agc ttt gtg gaa 355Pro Glu Gly Gln Leu Met Pro Ile Pro Glu Gly Tyr Ser Phe Val Glu70 75 80 85gcg gcc tcg atc gtg gag gtt gcg tgc acg gtg tgg tcg aat atc ggc 403Ala Ala Ser Ile Val Glu Val Ala Cys Thr Val Trp Ser Asn Ile Gly
90 95 100atg ctg gcg ggc ttg cag aag gag gat act ttc ctt att cat ggt ggc 451Met Leu Ala Gly Leu Gln Lys Glu Asp Thr Phe Leu Ile His Gly Gly
105 110 115gcg ggc ggt atc gga acg ttt gcc att cag atg ggc aag gct ctg ggt 499Ala Gly Gly Ile Gly Thr Phe Ala Ile Gln Met Gly Lys Ala Leu Gly
120 125 130gtg acg gtt gcg gtg act gcc ggt tca act gaa aag tta aaa acc tgt 547Val Thr Val Ala Val Thr Ala Gly Ser Thr Glu Lys Leu Lys Thr Cys
135 140 145aag aac tta ggg gcc gat atc ctc atc aat tac aag gag gaa gat ttc 595Lys Asn Leu Gly Ala Asp Ile Leu Ile Asn Tyr Lys Glu Glu Asp Phe150 155 160 165gcc gag gtt ttg aag aac aag gcg gat gtc att ctc gat att att ggt 643Ala Glu Val Leu Lys Asn Lys Ala Asp Val Ile Leu Asp Ile Ile Gly
170 175 180gcg aag tat ttg tca cag aat gtg aag gcg atg gcc aag gac gcg cac 691Ala Lys Tyr Leu Ser Gln Asn Val Lys Ala Met Ala Lys Asp Ala His
185 190 195atg gta gtc atc ggg atg cag ggt ggc gtg aaa ggg gag ctg aat ttg 739Met Val Val Ile Gly Met Gln Gly Gly Val Lys Gly Glu Leu Asn Leu
200 205 210ggt cat ctt ttg gcc aag cga ggc acg att tct gcc act gcg ctg cgt 787Gly His Leu Leu Ala Lys Arg Gly Thr Ile Ser Ala Thr Ala Leu Arg
215 220 225ggt cgc gat gag gcg gat aag gct cgg att gtc agc agc act gtc gaa 835Gly Arg Asp Glu Ala Asp Lys Ala Arg Ile Val Ser Ser Thr Val Glu230 235 240 245aat att tgg ccg ctg ctg caa tcg aag gaa att acc cct cac atc gac 883Asn Ile Trp Pro Leu Leu Gln Ser Lys Glu Ile Thr Pro His Ile Asp
250 255 260cac acc ttg ccg cta gcc gaa gca gcc gcc gcc ttg cag aaa att caa 931His Thr Leu Pro Leu Ala Glu Ala Ala Ala Ala Leu Gln Lys Ile Gln
265 270 275gac ggc acc atc acc ggc aag ctc gtg ctt gcg gtt taggcaagcg 977Asp Gly Thr Ile Thr Gly Lys Leu Val Leu Ala Val
280 285atgccagcac cct 990<210>404<211>289<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>404Val Leu Val Lys Val Lys Pro Ala Gly Val Asn Arg Ala Asp Leu Leu1 5 10 15Gln Thr Gln Gly Asn Tyr Pro Val Pro Ala Gly Ala Ser Glu Ile Leu
20 25 30Gly Leu Glu Cys Ala Gly Val Ile Val Asn Ala Gly Asp Thr Gly Gln
35 40 45Thr Val Gly Gln Glu Val Ala Cys Leu Leu Thr Gly Gly Gly Tyr Ala
50 55 60Gln Tyr Val Ala Val Pro Glu Gly Gln Leu Met Pro Ile Pro Glu Gly65 70 75 80Tyr Ser Phe Val Glu Ala Ala Ser Ile Val Glu Val Ala Cys Thr Val
85 90 95Trp Ser Asn Ile Gly Met Leu Ala Gly Leu Gln Lys Glu Asp Thr Phe
100 105 110Leu Ile His Gly Gly Ala Gly Gly Ile Gly Thr Phe Ala Ile Gln Met
115 120 125Gly Lys Ala Leu Gly Val Thr Val Ala Val Thr Ala Gly Ser Thr Glu
130 135 140Lys Leu Lys Thr Cys Lys Asn Leu Gly Ala Asp Ile Leu Ile Asn Tyr145 150 155 160Lys Glu Glu Asp Phe Ala Glu Val Leu Lys Asn Lys Ala Asp Val Ile
165 170 175Leu Asp Ile Ile Gly Ala Lys Tyr Leu Ser Gln Asn Val Lys Ala Met
180 185 190Ala Lys Asp Ala His Met Val Val Ile Gly Met Gln Gly Gly Val Lys
195 200 205Gly Glu Leu Asn Leu Gly His Leu Leu Ala Lys Arg Gly Thr Ile Ser
210 215 220Ala Thr Ala Leu Arg Gly Arg Asp Glu Ala Asp Lys Ala Arg Ile Val225 230 235 240Ser Ser Thr Val Glu Asn Ile Trp Pro Leu Leu Gln Ser Lys Glu Ile
245 250 255Thr Pro His Ile Asp His Thr Leu Pro Leu Ala Glu Ala Ala Ala Ala
260 265 270Leu Gln Lys Ile Gln Asp Gly Thr Ile Thr Gly Lys Leu Val Leu Ala
275 280 285Val<210>405<211>1098<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1075)<223>RXN01619<400>405cctgcaagtt tactgctcgg ccgtcacggg ggaatggaaa aagtacgctt ggtgttcata 60tagcgaaccc attttctatt gcgatgagag gaacaccacc atg cgc gca atc act 115
Met Arg Ala Ile Thr
1 5cac aac act ttc ggc gac ccc gcc gac gtc cta cag att acc gag aag 163His Asn Thr Phe Gly Asp Pro Ala Asp Val Leu Gln Ile Thr Glu Lys
10 15 20gaa att ccc act ccc ggc cca ggt cag gtt cgt att caa gtg acg ctg 211Glu Ile Pro Thr Pro Gly Pro Gly Gln Val Arg Ile Gln Val Thr Leu
25 30 35gca acc atc cac aac cat gat ttg tgg acc gtg aag ggc tct tac ggc 259Ala Thr Ile His Asn His Asp Leu Trp Thr Val Lys Gly Ser Tyr Gly
40 45 50ttc gtc cca gat ctg ccg gcc gcc gca ggc acc gag gca gtc ggc atc 307Phe Val Pro Asp Leu Pro Ala Ala Ala Gly Thr Glu Ala Val Gly Ile
55 60 65gtc gac gcc ctg ggc gag ggc gtc gaa ggt ttg cag gtc ggt cag cgt 355Val Asp Ala Leu Gly Glu Gly Val Glu Gly Leu Gln Val Gly Gln Arg70 75 80 85gtt gcg tcc ggc acc agc ttt ggc atc tgg gcg gag tac gcg ctt gtc 403Val Ala Ser Gly Thr Ser Phe Gly Ile Trp Ala Glu Tyr Ala Leu Val
90 95 100gac gcc tcc ggc ctc att ccc gta cca gaa cag ctc tcc gac gaa agc 451Asp Ala Ser Gly Leu Ile Pro Val Pro Glu Gln Leu Ser Asp Glu Ser
105 110 115gca gct cag ctc gtc gca atg cct ttc agc gcc atc agc ctt ctt gat 499Ala Ala Gln Leu Val Ala Met Pro Phe Ser Ala Ile Ser Leu Leu Asp
120 125 130ttc ctg gat atg aaa cca ggg gag tgg ctg atc caa aac tcc gca aac 547Phe Leu Asp Met Lys Pro Gly Glu Trp Leu Ile Gln Asn Ser Ala Asn
135 140 145ggt gcc gtc ggc cgc atg ctc gca cag ctg gca gaa tcc cgc ggc atc 595Gly Ala Val Gly Arg Met Leu Ala Gln Leu Ala Glu Ser Arg Gly Ile150 155 160 165cat gtc gtt ggt ctc gtc cgc cgt gac gcc ggt gtc caa gaa ctc gct 643His Val Val Gly Leu Val Arg Arg Asp Ala Gly Val Gln Glu Leu Ala
170 175 180gct caa aac atc agc ggc gtc gtt tcc act gag acc cca ggc tgg gaa 691Ala Gln Asn Ile Ser Gly Val Val Ser Thr Glu Thr Pro Gly Trp Glu
185 190 195aag cag gtc gaa gac atc acc ggt ggc gca agc atc gcc gtc gca ctt 739Lys Gln Val Glu Asp Ile Thr Gly Gly Ala Ser Ile Ala Val Ala Leu
200 205 210gat tcc gtc ggt gga tcc tcc gca gct gac ctg gtg aaa ctg ctt ggc 787Asp Ser Val Gly Gly Ser Ser Ala Ala Asp Leu Val Lys Leu Leu Gly
215 220 225gaa ggc ggc acc ctc gtc tcc ttc ggc gcc atg ggc aac cca atc atg 835Glu Gly Gly Thr Leu Val Ser Phe Gly Ala Met Gly Asn Pro Ile Met230 235 240 245gaa atc cca tcc ggc ccc gtc atc ttc aag cac atc acc gtc aag ggc 883Glu Ile Pro Ser Gly Pro Val Ile Phe Lys His Ile Thr Val Lys Gly
250 255 260ttc tgg gga agc aaa gtc agc cgc gaa atg cca gca gag aag aaa acc 931Phe Trp Gly Ser Lys Val Ser Arg Glu Met Pro Ala Glu Lys Lys Thr
265 270 275cag ttg ttc ggc gag ctc att gcg cgc ata ctt gat gga aca ttg acc 979Gln Leu Phe Gly Glu Leu Ile Ala Arg Ile Leu Asp Gly Thr Leu Thr
280 285 290ctt cca gtt gat tcc acc ttt gat gcc gct gac atc gtc tcg gcc gtg 1027Leu Pro Val Asp Ser Thr Phe Asp Ala Ala Asp Ile Val Ser Ala Val
295 300 305cgc gcc tcc agc gag cct ggc cgt gcc gga aaa gtg ctc att cgt ttc 1075Arg Ala Ser Ser Glu Pro Gly Arg Ala Gly Lys Val Leu Ile Arg Phe310 315 320 325taaacgttta aggcccatta gac 1098<210>406<211>325<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>406Met Arg Ala Ile Thr His Asn Thr Phe Gly Asp Pro Ala Asp Val Leu1 5 10 15Gln Ile Thr Glu Lys Glu Ile Pro Thr Pro Gly Pro Gly Gln Val Arg
20 25 30Ile Gln Val Thr Leu Ala Thr Ile His Asn His Asp Leu Trp Thr Val
35 40 45Lys Gly Ser Tyr Gly Phe Val Pro Asp Leu Pro Ala Ala Ala Gly Thr
50 55 60Glu Ala Val Gly Ile Val Asp Ala Leu Gly Glu Gly Val Glu Gly Leu65 70 75 80Gln Val Gly Gln Arg Val Ala Ser Gly Thr Ser Phe Gly Ile Trp Ala
85 90 95Glu Tyr Ala Leu Val Asp Ala Ser Gly Leu Ile Pro Val Pro Glu Gln
100 105 110Leu Ser Asp Glu Ser Ala Ala Gln Leu Val Ala Met Pro Phe Ser Ala
115 120 125Ile Ser Leu Leu Asp Phe Leu Asp Met Lys Pro Gly Glu Trp Leu Ile
130 135 140Gln Asn Ser Ala Asn Gly Ala Val Gly Arg Met Leu Ala Gln Leu Ala145 150 155 160Glu Ser Arg Gly Ile His Val Val Gly Leu Val Arg Arg Asp Ala Gly
165 170 175Val Gln Glu Leu Ala Ala Gln Asn Ile Ser Gly Val Val Ser Thr Glu
180 185 190Thr Pro Gly Trp Glu Lys Gln Val Glu Asp Ile Thr Gly Gly Ala Ser
195 200 205Ile Ala Val Ala Leu Asp Ser Val Gly Gly Ser Ser Ala Ala Asp Leu
210 215 220Val Lys Leu Leu Gly Glu Gly Gly Thr Leu Val Ser Phe Gly Ala Met225 230 235 240Gly Asn Pro Ile Met Glu Ile Pro Ser Gly Pro Val Ile Phe Lys His
245 250 255Ile Thr Val Lys Gly Phe Trp Gly Ser Lys Val Ser Arg Glu Met Pro
260 265 270Ala Glu Lys Lys Thr Gln Leu Phe Gly Glu Leu Ile Ala Arg Ile Leu
275 280 285Asp Gly Thr Leu Thr Leu Pro Val Asp Ser Thr Phe Asp Ala Ala Asp
290 295 300Ile Val Ser Ala Val Arg Ala Ser Ser Glu Pro Gly Arg Ala Gly Lys305 310 315 320Val Leu Ile Arg Phe
325<210>407<211>1041<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1018)<223>RXN01842<400>407cacgtcatgg tctcgcggtg atcatcttcc taccgtgaca ggcaaggccg caaacggccg 60tgaccacagg aaagaaattc acgaggagag gaagcacacg atg tcg aag gta tac 115
Met Ser Lys Val Tyr
1 5gtg tcc aac gag tac ggc ggc ccg gaa aac cag gaa ctg atc acc cgc 163Val Ser Asn Glu Tyr Gly Gly Pro Glu Asn Gln Glu Leu Ile Thr Arg
10 15 20aac acc ccc cag cca ggc ccg gga gaa ctc ggg gtc aag gtc cac gcg 211Asn Thr Pro Gln Pro Gly Pro Gly Glu Leu Gly Val Lys Val His Ala
25 30 35gcc ggg gtc aac ccg ctt gat tgg aag gtc cgt tcc ggg gtt gcc gga 259Ala Gly Val Asn Pro Leu Asp Trp Lys Val Arg Ser Gly Val Ala Gly
40 45 50acc ccg cga gag ctt ccg gca ccc ctg ggc gag gag gcc tcc ggg atc 307Thr Pro Arg Glu Leu Pro Ala Pro Leu Gly Glu Glu Ala Ser Gly Ile
55 60 65gtc acc gcc gtt gga gac ggt gtg gag ggc ttc gcg gtc ggc gat ccg 355Val Thr Ala Val Gly Asp Gly Val Glu Gly Phe Ala Val Gly Asp Pro70 75 80 85gtg ctc ggc ctg gtg gcc ccc ggc gtc ggc gga tat gcc gag gac acc 403Val Leu Gly Leu Val Ala Pro Gly Val Gly Gly Tyr Ala Glu Asp Thr
90 95 100ctg ctg gtg gca gag agt acc gtg cta aag ccg gag gag atc tcg ttc 451Leu Leu Val Ala Glu Ser Thr Val Leu Lys Pro Glu Glu Ile Ser Phe
105 110 115acc gac gcc gcc gcg atc ccg gtc gct ggg gcg agc gcc tac gcc ggc 499Thr Asp Ala Ala Ala Ile Pro Val Ala Gly Ala Ser Ala Tyr Ala Gly
120 125 130act cac cag gtc gag ctt gaa cca ggc cag tcg ttg ctg atc aat ggg 547Thr His Gln Val Glu Leu Glu Pro Gly Gln Ser Leu Leu Ile Asn Gly
135 140 145gcc ggt ggt ggg gtc ggg ctg atg gcc gcg cag atc gga cgg gtc cac 595Ala Gly Gly Gly Val Gly Leu Met Ala Ala Gln Ile Gly Arg Val His150 155 160 165aag ttc cag gtc gtc ggc gtt gac cac gag gac aag cgc gag ctc atc 643Lys Phe Gln Val Val Gly Val Asp His Glu Asp Lys Arg Glu Leu Ile
170 175 180gaa tcc acc ggt gct atc ttc gtc gcc acc ggc gac gcc gtc gcg gag 691Glu Ser Thr Gly Ala Ile Phe Val Ala Thr Gly Asp Ala Val Ala Glu
185 190 195cag gtg cgt gcg ctg ctc cct gac ggt gtg gac gta gtc ttc gac cta 739Gln Val Arg Ala Leu Leu Pro Asp Gly Val Asp Val Val Phe Asp Leu
200 205 210gtc ggc ggg gag gcg ttg cgg gtg gtt gct ccc tta gcg aag aat ccg 787Val Gly Gly Glu Ala Leu Arg Val Val Ala Pro Leu Ala Lys Asn Pro
215 220 225gcg cac gtg atc tcg gcg gct gat gct gcc acc gtg gga gaa ctc ggt 835Ala His Val Ile Ser Ala Ala Asp Ala Ala Thr Val Gly Glu Leu Gly230 235 240 245gga cag gtg ctg cgc cgc acc ccg gaa atg gtc gga cag atc acc ggg 883Gly Gln Val Leu Arg Arg Thr Pro Glu Met Val Gly Gln Ile Thr Gly
250 255 260gtg gtc cag tac ggg ctg gtc gac ccg aag gtc gat acg acc tac ccg 931Val Val Gln Tyr Gly Leu Val Asp Pro Lys Val Asp Thr Thr Tyr Pro
265 270 275ctg gaa cag gcc ggt aag gcc ctg gcc cac gtt gag cag ggc cac gcc 979Leu Glu Gln Ala Gly Lys Ala Leu Ala His Val Glu Gln Gly His Ala
280 285 290cgc ggc aag atc gtc ctc gag ctc atc acc tcc cag gac taaccagaca 1028Arg Gly Lys Ile Val Leu Glu Leu Ile Thr Ser Gln Asp
295 300 305acgcggtgac ctc 1041<210>408<211>306<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>408Met Ser Lys Val Tyr Val Ser Asn Glu Tyr Gly Gly Pro Glu Asn Gln1 5 10 15Glu Leu Ile Thr Arg Asn Thr Pro Gln Pro Gly Pro Gly Glu Leu Gly
20 25 30Val Lys Val His Ala Ala Gly Val Asn Pro Leu Asp Trp Lys Val Arg
35 40 45Ser Gly Val Ala Gly Thr Pro Arg Glu Leu Pro Ala Pro Leu Gly Glu
50 55 60Glu Ala Ser Gly Ile Val Thr Ala Val Gly Asp Gly Val Glu Gly Phe65 70 75 80Ala Val Gly Asp Pro Val Leu Gly Leu Val Ala Pro Gly Val Gly Gly
85 90 95Tyr Ala Glu Asp Thr Leu Leu Val Ala Glu Ser Thr Val Leu Lys Pro
100 105 110Glu Glu Ile Ser Phe Thr Asp Ala Ala Ala Ile Pro Val Ala Gly Ala
115 120 125Ser Ala Tyr Ala Gly Thr His Gln Val Glu Leu Glu Pro Gly Gln Ser
130 135 140Leu Leu Ile Asn Gly Ala Gly Gly Gly Val Gly Leu Met Ala Ala Gln145 150 155 160Ile Gly Arg Val His Lys Phe Gln Val Val Gly Val Asp His Glu Asp
165 170 175Lys Arg Glu Leu Ile Glu Ser Thr Gly Ala Ile Phe Val Ala Thr Gly
180 185 190Asp Ala Val Ala Glu Gln Val Arg Ala Leu Leu Pro Asp Gly Val Asp
195 200 205Val Val Phe Asp Leu Val Gly Gly Glu Ala Leu Arg Val Val Ala Pro
210 215 220Leu Ala Lys Asn Pro Ala His Val Ile Ser Ala Ala Asp Ala Ala Thr225 230 235 240Val Gly Glu Leu Gly Gly Gln Val Leu Arg Arg Thr Pro Glu Met Val
245 250 255Gly Gln Ile Thr Gly Val Val Gln Tyr Gly Leu Val Asp Pro Lys Val
260 265 270Asp Thr Thr Tyr Pro Leu Glu Gln Ala Gly Lys Ala Leu Ala His Val
275 280 285Glu Gln Gly His Ala Arg Gly Lys Ile Val Leu Glu Leu Ile Thr Ser
290 295 300Gln Asp305<210>409<211>1614<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1591)<223>RXN00641<400>409tgcggaattg ctcgcaaatg tcacacaccg cttcaaagca aaaacgaaaa cgacatcgcg 60gtggcaatac caacttcttt tcactctctt ggaggttcac atg tcc aca cca gtt 115
Met Ser Thr Pro Val
1 5tca aat ttg gca agc gtt cag aaa act ctg gac cat gcg ctt gag gac 163Ser Asn Leu Ala Ser Val Gln Lys Thr Leu Asp His Ala Leu Glu Asp
10 15 20cgc cct gaa gag gga atc gtc cgc gtc aac cgc aac atc ttc act gac 211Arg Pro Glu Glu Gly Ile Val Arg Val Asn Arg Asn Ile Phe Thr Asp
25 30 35cct gag atc ttc gag ctg gag atg cgc cac atc ttc gaa ggc atc tgg 259Pro Glu Ile Phe Glu Leu Glu Met Arg His Ile Phe Glu Gly Ile Trp
40 45 50atg gac atg gct cac gag tcc cag atc cct aac ggt gga gac tac ttc 307Met Asp Met Ala His Glu Ser Gln Ile Pro Asn Gly Gly Asp Tyr Phe
55 60 65acc acc tac att ggc tgc cag cgg atc atg atc acc cgt tcc aag gaa 355Thr Thr Tyr Ile Gly Cys Gln Arg Ile Met Ile Thr Arg Ser Lys Glu70 75 80 85ggc aca ctc aac ggc ctg atc aac gcg tgt tct cac cgt ggc gcc atg 403Gly Thr Leu Asn Gly Leu Ile Asn Ala Cys Ser His Arg Gly Ala Met
90 95 100ctc tgc cgt ggc aag agt gac aac cgc acc tcc ttg acc tgc cca ttc 451Leu Cys Arg Gly Lys Ser Asp Asn Arg Thr Ser Leu Thr Cys Pro Phe
105 110 115cac ggc tgg cca ttc tgc aac ggc ggc gca ctg ctc aag gtc aag ggc 499His Gly Trp Pro Phe Cys Asn Gly Gly Ala Leu Leu Lys Val Lys Gly
120 125 130gaa aaa gaa ggc gcc tac cca gag aat ttc cgc acc gac ggc tcc cac 547Glu Lys Glu Gly Ala Tyr Pro Glu Asn Phe Arg Thr Asp Gly Ser His
135 140 145gat gtg cgt cgc gtt cct aag tta gag tcc tac cgt ggc ttc ctc ttc 595Asp Val Arg Arg Val Pro Lys Leu Glu Ser Tyr Arg Gly Phe Leu Phe150 155 160 165ggc tcc ctc aac gat gat gtc gtt tct ttg gaa gag cac ctc ggc gac 643Gly Ser Leu Asn Asp Asp Val Val Ser Leu Glu Glu His Leu Gly Asp
170 175 180acc cgt acc gtc att gac atg ctg gtt gac caa tcc cca gaa ggc ctc 691Thr Arg Thr Val Ile Asp Met Leu Val Asp Gln Ser Pro Glu Gly Leu
185 190 195gaa gta ctg cgc gga tcc tcc acc tac acc tac gac ggc aac tgg aag 739Glu Val Leu Arg Gly Ser Ser Thr Tyr Thr Tyr Asp Gly Asn Trp Lys
200 205 210ctc cag acc gaa aac ggt gca gac ggc tac cac gtt tcc tcc acc cac 787Leu Gln Thr Glu Asn Gly Ala Asp Gly Tyr His Val Ser Ser Thr His
215 220 225tgg aac tac gct gca acc acc tcc cgc cgt ggc act ggt gaa tcc gcg 835Trp Asn Tyr Ala Ala Thr Thr Ser Arg Arg Gly Thr Gly Glu Ser Ala230 235 240 245aac gaa acc aag gca atg gac gct ggt acc tgg ggc aag cag ggt ggc 883Asn Glu Thr Lys Ala Met Asp Ala Gly Thr Trp Gly Lys Gln Gly Gly
250 255 260gga tac ttc tcc tac cct tac ggc cac atg ctg ctg tgg atg tgg tgg 931Gly Tyr Phe Ser Tyr Pro Tyr Gly His Met Leu Leu Trp Met Trp Trp
265 270 275ggc aac cca gaa gac cgc cca ctg ttc gag cgc cgc gac gag ttc aag 979Gly Asn Pro Glu Asp Arg Pro Leu Phe Glu Arg Arg Asp Glu Phe Lys
280 285 290aag gaa ttc ggc gaa gaa aag ggc gag ttc atg gtt ggt gct tcc cgc 1027Lys Glu Phe Gly Glu Glu Lys Gly Glu Phe Met Val Gly Ala Ser Arg
295 300 305aat ctg tgc ctc tac ccc aat gtt tac ctg atg gat cag ttc tcc tca 1075Asn Leu Cys Leu Tyr Pro Asn Val Tyr Leu Met Asp Gln Phe Ser Ser310 315 320 325cag atc cgc cac atc cgc cca atc tca gtt gat cag acc gaa gtc acc 1123Gln Ile Arg His Ile Arg Pro Ile Ser Val Asp Gln Thr Glu Val Thr
330 335 340atc tac tgc atc gca cct aag ggc gag tcc gcg gaa gca cgt gca aac 1171Ile Tyr Cys Ile Ala Pro Lys Gly Glu Ser Ala Glu Ala Arg Ala Asn
345 350 355cgc atc cgc cag tac gaa gac ttc ttc aac gca acg ggc atg gca acc 1219Arg Ile Arg Gln Tyr Glu Asp Phe Phe Asn Ala Thr Gly Met Ala Thr
360 365 370cca gat gac ctg gag gaa ttc cgc tcc tgc cag aag acc tac cag gca 1267Pro Asp Asp Leu Glu Glu Phe Arg Ser Cys Gln Lys Thr Tyr Gln Ala
375 380 385tct gcc ttc cca tgg aat gac atg acc cgc ggt ttg ggc cac cag gta 1315Ser Ala Phe Pro Trp Asn Asp Met Thr Arg Gly Leu Gly His Gln Val390 395 400 405cag gga cca aac gag gtt gcc aag ggc cta ggc atg aac gaa gtt ctt 1363Gln Gly Pro Asn Glu Val Ala Lys Gly Leu Gly Met Asn Glu Val Leu
410 415 420tcc tcc gga gca cgc acc gaa gat gaa ggc ctc tac cca atc cag cac 1411Ser Ser Gly Ala Arg Thr Glu Asp Glu Gly Leu Tyr Pro Ile Gln His
425 430 435ggc ttc tgg cat gaa ctc atg cag gag gct gtg aat aag cag agc atc 1459Gly Phe Trp His Glu Leu Met Gln Glu Ala Val Asn Lys Gln Ser Ile
440 445 450aag gaa aag gaa ttg gct gac gat acc gct tct tcc ctt gcc acc gta 1507Lys Glu Lys Glu Leu Ala Asp Asp Thr Ala Ser Ser Leu Ala Thr Val
455 460 465gct gca gcc aaa atc cgt gag gaa gca aag gca gcc gcg aag tcc gac 1555Ala Ala Ala Lys Ile Arg Glu Glu Ala Lys Ala Ala Ala Lys Ser Asp470 475 480 485gct gga gag cct cgc cgc cgt cgt cgc acc cgc ggt tagtcgtcga 1601Ala Gly Glu Pro Arg Arg Arg Arg Arg Thr Arg Gly
490 495aaagcaaaaa atc 1614<210>410<211>497<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>410Met Ser Thr Pro Val Ser Asn Leu Ala Ser Val Gln Lys Thr Leu Asp1 5 10 15His Ala Leu Glu Asp Arg Pro Glu Glu Gly Ile Val Arg Val Asn Arg
20 25 30Asn Ile Phe Thr Asp Pro Glu Ile Phe Glu Leu Glu Met Arg His Ile
35 40 45Phe Glu Gly Ile Trp Met Asp Met Ala His Glu Ser Gln Ile Pro Asn
50 55 60Gly Gly Asp Tyr Phe Thr Thr Tyr Ile Gly Cys Gln Arg Ile Met Ile65 70 75 80Thr Arg Ser Lys Glu Gly Thr Leu Asn Gly Leu Ile Asn Ala Cys Ser
85 90 95His Arg Gly Ala Met Leu Cys Arg Gly Lys Ser Asp Asn Arg Thr Ser
100 105 110Leu Thr Cys Pro Phe His Gly Trp Pro Phe Cys Asn Gly Gly Ala Leu
115 120 125Leu Lys Val Lys Gly Glu Lys Glu Gly Ala Tyr Pro Glu Asn Phe Arg
130 135 140Thr Asp Gly Ser His Asp Val Arg Arg Val Pro Lys Leu Glu Ser Tyr145 150 155 160Arg Gly Phe Leu Phe Gly Ser Leu Asn Asp Asp Val Val Ser Leu Glu
165 170 175Glu His Leu Gly Asp Thr Arg Thr Val Ile Asp Met Leu Val Asp Gln
180 185 190Ser Pro Glu Gly Leu Glu Val Leu Arg Gly Ser Ser Thr Tyr Thr Tyr
195 200 205Asp Gly Asn Trp Lys Leu Gln Thr Glu Asn Gly Ala Asp Gly Tyr His
210 215 220Val Ser Ser Thr His Trp Asn Tyr Ala Ala Thr Thr Ser Arg Arg Gly225 230 235 240Thr Gly Glu Ser Ala Asn Glu Thr Lys Ala Met Asp Ala Gly Thr Trp
245 250 255Gly Lys Gln Gly Gly Gly Tyr Phe Ser Tyr Pro Tyr Gly His Met Leu
260 265 270Leu Trp Met Trp Trp Gly Asn Pro Glu Asp Arg Pro Leu Phe Glu Arg
275 280 285Arg Asp Glu Phe Lys Lys Glu Phe Gly Glu Glu Lys Gly Glu Phe Met
290 295 300Val Gly Ala Ser Arg Asn Leu Cys Leu Tyr Pro Asn Val Tyr Leu Met305 310 315 320Asp Gln Phe Ser Ser Gln Ile Arg His Ile Arg Pro Ile Ser Val Asp
325 330 335Gln Thr Glu Val Thr Ile Tyr Cys Ile Ala Pro Lys Gly Glu Ser Ala
340 345 350Glu Ala Arg Ala Asn Arg Ile Arg Gln Tyr Glu Asp Phe Phe Asn Ala
355 360 365Thr Gly Met Ala Thr Pro Asp Asp Leu Glu Glu Phe Arg Ser Cys Gln
370 375 380Lys Thr Tyr Gln Ala Ser Ala Phe Pro Trp Asn Asp Met Thr Arg Gly385 390 395 400Leu Gly His Gln Val Gln Gly Pro Asn Glu Val Ala Lys Gly Leu Gly
405 410 415Met Asn Glu Val Leu Ser Ser Gly Ala Arg Thr Glu Asp Glu Gly Leu
420 425 430Tyr Pro Ile Gln His Gly Phe Trp His Glu Leu Met Gln Glu Ala Val
435 440 445Asn Lys Gln Ser Ile Lys Glu Lys Glu Leu Ala Asp Asp Thr Ala Ser
450 455 460Ser Leu Ala Thr Val Ala Ala Ala Lys Ile Arg Glu Glu Ala Lys Ala465 470 475 480Ala Ala Lys Ser Asp Ala Gly Glu Pro Arg Arg Arg Arg Arg Thr Arg
485 490 495Gly<210>411<211>1166<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(16)..(1143)<223>RXN01993<400>411ctttaggagt tcacc atg aca ctg tcc gaa cgc aag ctc acc acc acc gcc 51
Met Thr Leu Ser Glu Arg Lys Leu Thr Thr Thr Ala
1 5 10aag att ctt ccc cac cca ctc aac gcc tgg tac gtc gcc gct tgg gat 99Lys Ile Leu Pro His Pro Leu Asn Ala Trp Tyr Val Ala Ala Trp Asp
15 20 25tat gaa gtc aca tct aaa aag ccc atg gcc agg aca atc gcc aac aaa 147Tyr Glu Val Thr Ser Lys Lys Pro Met Ala Arg Thr Ile Ala Asn Lys
30 35 40cca ctc gct ttg tac cgc acc aaa gat ggc cga gcc gtt gcc ctt gca 195Pro Leu Ala Leu Tyr Arg Thr Lys Asp Gly Arg Ala Val Ala Leu Ala45 50 55 60gac gcc tgc tgg cac cgc ctc gca ccg cta tcc aag gga aaa ctc gtg 243Asp Ala Cys Trp His Arg Leu Ala Pro Leu Ser Lys Gly Lys Leu Val
65 70 75ggc aca gac gga atc caa tgc cct tat cac ggc ttg gag tac aac tcc 291Gly Thr Asp Gly Ile Gln Cys Pro Tyr His Gly Leu Glu Tyr Asn Ser
80 85 90gcg ggc cgc tgc atg aaa atg ccc gcg cag gaa acc ctc aac ccg tca 339Ala Gly Arg Cys Met Lys Met Pro Ala Gln Glu Thr Leu Asn Pro Ser
95 100 105gca gcc gtc aac tcc tac ccc gtg gtg gaa gcc cac cgc ttt gtg tgg 387Ala Ala Val Asn Ser Tyr Pro Val Val Glu Ala His Arg Phe Val Trp
110 115 120gtg tgg ctg ggc gat ccc aca ttg gca gat ccc acc caa gta ccc gat 435Val Trp Leu Gly Asp Pro Thr Leu Ala Asp Pro Thr Gln Val Pro Asp125 130 135 140atg cac cag atg agc cac ccc gaa tgg gca ggc gat gga cgc acc atc 483Met His Gln Met Ser His Pro Glu Trp Ala Gly Asp Gly Arg Thr Ile
145 150 155tcc gct gac tgc aac tac caa tta gtg ctg gac aac ttg atg gac ctc 531Ser Ala Asp Cys Asn Tyr Gln Leu Val Leu Asp Asn Leu Met Asp Leu
160 165 170acc cac gaa gag ttc gtg cac tcc tcc agc atc gga caa gac gaa ctt 579Thr His Glu Glu Phe Val His Ser Ser Ser Ile Gly Gln Asp Glu Leu
175 180 185agt gaa tca gag ttc gtg gtc acc cac act gaa gat tcc gtg acg gtc 627Ser Glu Ser Glu Phe Val Val Thr His Thr Glu Asp Ser Val Thr Val
190 195 200acc cgc tgg atg cat gac ata gat gca cca ccg ttt tgg caa aag aac 675Thr Arg Trp Met His Asp Ile Asp Ala Pro Pro Phe Trp Gln Lys Asn205 210 215 220atg aat gat aag ttc cca gga ttt gaa ggc aag gtg gat cgt tgg cag 723Met Asn Asp Lys Phe Pro Gly Phe Glu Gly Lys Val Asp Arg Trp Gln
225 230 235atc atc cac tac tac tac cct tcc acc atc tgc att gat gtt ggt gta 771Ile Ile His Tyr Tyr Tyr Pro Ser Thr Ile Cys Ile Asp Val Gly Val
240 245 250gca aag gct gga act ggc gcg cag gaa ggc gac cgc agc cag ggc gtt 819Ala Lys Ala Gly Thr Gly Ala Gln Glu Gly Asp Arg Ser Gln Gly Val
255 260 265aat ggg tat gtc atg aac acc att acc cca gat tca gat cgt tcc tct 867Asn Gly Tyr Val Met Asn Thr Ile Thr Pro Asp Ser Asp Arg Ser Ser
270 275 280cat tac ttc tgg gca ttc atg cgc aac tac cgc ctg gaa agc caa acc 915His Tyr Phe Trp Ala Phe Met Arg Asn Tyr Arg Leu Glu Ser Gln Thr285 290 295 300atc acc acc cag ctg cgc gac ggt gta tcc ggt gta ttc aaa gaa gac 963Ile Thr Thr Gln Leu Arg Asp Gly Val Ser Gly Val Phe Lys Glu Asp
305 310 315gaa gac atg ctg acc gct cag caa gat gcc atc gac gcc aac acc gac 1011Glu Asp Met Leu Thr Ala Gln Gln Asp Ala Ile Asp Ala Asn Thr Asp
320 325 330tac gag ttt tac agc ctc aac att gat gcc ggt ggc atg tgg gtg cgc 1059Tyr Glu Phe Tyr Ser Leu Asn Ile Asp Ala Gly Gly Met Trp Val Arg
335 340 345cga atc ctc gag gaa gca ctc tcc aag gaa ggc cga ctg gat atc ccc 1107Arg Ile Leu Glu Glu Ala Leu Ser Lys Glu Gly Arg Leu Asp Ile Pro
350 355 360acc aca ttc ccc cgc gca aca ccg aag ccg gag gca taaaccatga 1153Thr Thr Phe Pro Arg Ala Thr Pro Lys Pro Glu Ala365 370 375actcgcaatg gca 1166<210>412<211>376<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>412Met Thr Leu Ser Glu Arg Lys Leu Thr Thr Thr Ala Lys Ile Leu Pro1 5 10 15His Pro Leu Asn Ala Trp Tyr Val Ala Ala Trp Asp Tyr Glu Val Thr
20 25 30Ser Lys Lys Pro Met Ala Arg Thr Ile Ala Asn Lys Pro Leu Ala Leu
35 40 45Tyr Arg Thr Lys Asp Gly Arg Ala Val Ala Leu Ala Asp Ala Cys Trp
50 55 60His Arg Leu Ala Pro Leu Ser Lys Gly Lys Leu Val Gly Thr Asp Gly65 70 75 80Ile Gln Cys Pro Tyr His Gly Leu Glu Tyr Asn Ser Ala Gly Arg Cys
85 90 95Met Lys Met Pro Ala Gln Glu Thr Leu Asn Pro Ser Ala Ala Val Asn
100 105 110Ser Tyr Pro Val Val Glu Ala His Arg Phe Val Trp Val Trp Leu Gly
115 120 125Asp Pro Thr Leu Ala Asp Pro Thr Gln Val Pro Asp Met His Gln Met
130 135 140Ser His Pro Glu Trp Ala Gly Asp Gly Arg Thr Ile Ser Ala Asp Cys145 150 155 160Asn Tyr Gln Leu Val Leu Asp Asn Leu Met Asp Leu Thr His Glu Glu
165 170 175Phe Val His Ser Ser Ser Ile Gly Gln Asp Glu Leu Ser Glu Ser Glu
180 185 190Phe Val Val Thr His Thr Glu Asp Ser Val Thr Val Thr Arg Trp Met
195 200 205His Asp Ile Asp Ala Pro Pro Phe Trp Gln Lys Asn Met Asn Asp Lys
210 215 220Phe Pro Gly Phe Glu Gly Lys Val Asp Arg Trp Gln Ile Ile His Tyr225 230 235 240Tyr Tyr Pro Ser Thr Ile Cys Ile Asp Val Gly Val Ala Lys Ala Gly
245 250 255Thr Gly Ala Gln Glu Gly Asp Arg Ser Gln Gly Val Asn Gly Tyr Val
260 265 270Met Asn Thr Ile Thr Pro Asp Ser Asp Arg Ser Ser His Tyr Phe Trp
275 280 285Ala Phe Met Arg Asn Tyr Arg Leu Glu Ser Gln Thr Ile Thr Thr Gln
290 295 300Leu Arg Asp Gly Val Ser Gly Val Phe Lys Glu Asp Glu Asp Met Leu305 310 315 320Thr Ala Gln Gln Asp Ala Ile Asp Ala Asn Thr Asp Tyr Glu Phe Tyr
325 330 335Ser Leu Asn Ile Asp Ala Gly Gly Met Trp Val Arg Arg Ile Leu Glu
340 345 350Glu Ala Leu Ser Lys Glu Gly Arg Leu Asp Ile Pro Thr Thr Phe Pro
355 360 365Arg Ala Thr Pro Lys Pro Glu Ala
370 375<210>413<211>816<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(793)<223>RXN00658<400>413cattgacacc cacaggttta ccagcatcac ggaaagtttg gatggatttt tactccggcc 60acaacgtctg gctggaagct cagccacgtg ctttctggtc gtg cgc cac gac gag 115
Val Arg His Asp Glu
1 5cac tac cca gct gcg gca aac ctc att gct ttc gat aag gga tgg tcc 163His Tyr Pro Ala Ala Ala Asn Leu Ile Ala Phe Asp Lys Gly Trp Ser
10 15 20acc ctc atc gcc cct cag ctg gaa gat cca gag gcg gag gag ttc acc 211Thr Leu Ile Ala Pro Gln Leu Glu Asp Pro Glu Ala Glu Glu Phe Thr
25 30 35gcc gga ttc ctc acc gaa tac cag gac aat ctg atc act gcg ggc atg 259Ala Gly Phe Leu Thr Glu Tyr Gln Asp Asn Leu Ile Thr Ala Gly Met
40 45 50gag cac cag gcg crc gcg agc ggc ttc ccg gtg ggg cgt cgc ttc aag 307Glu His Gln Ala Leu Ala Ser Gly Phe Pro Val Gly Arg Arg Phe Lys
55 60 65tcc gat att gct tta cga cgc tgc gat gcg gtg acc acc cac atc ggc 355Ser Asp Ile Ala Leu Arg Arg Cys Asp Ala Val Thr Thr His Ile Gly70 75 80 85cac gaa cac tcc gcc gat ggt cac tgg agg atc tac gta ttc gct ggc 403His Glu His Ser Ala Asp Gly His Trp Arg Ile Tyr Val Phe Ala Gly
90 95 100caa gcc acc cca caa gat tcc gag tct gca ctg aac aag tgg gcg cag 451Gln Ala Thr Pro Gln Asp Ser Glu Ser Ala Leu Asn Lys Trp Ala Gln
105 110 115tgg atg gag gaa agc gaa gac tca cca ctc aac cgc ttc acc cca gaa 499Trp Met Glu Glu Ser Glu Asp Ser Pro Leu Asn Arg Phe Thr Pro Glu
120 125 130gcc ggc gac cgc aac gca gtc ttc gat atc aag gct acc tac cag cag 547Ala Gly Asp Arg Asn Ala Val Phe Asp Ile Lys Ala Thr Tyr Gln Gln
135 140 145cat tac cac tcc ttc gac ctg ttc gat gcg cca gag gtc ttc ttc cca 595His Tyr His Ser Phe Asp Leu Phe Asp Ala Pro Glu Val Phe Phe Pro150 155 160 165cga gtt gga cca tac aag ctg caa aac ctc gaa aac gtt tgg acc gca 643Arg Val Gly Pro Tyr Lys Leu Gln Asn Leu Glu Asn Val Trp Thr Ala
170 175 180ctg gat tcc caa gac atc ttt gag tcc cgt ggc atc agt cgc gat ggc 691Leu Asp Ser Gln Asp Ile Phe Glu Ser Arg Gly Ile Ser Arg Asp Gly
185 190 195gca att gtt gtc gtt cgc cca gac cag tac gtc gca gca gtc ctc cca 739Ala Ile Val Val Val Arg Pro Asp Gln Tyr Val Ala Ala Val Leu Pro
200 205 210ctc gaa gac acc gca gca ctg gct gag ttc ttc aat ggc aat ctg ctt 787Leu Glu Asp Thr Ala Ala Leu Ala Glu Phe Phe Asn Gly Asn Leu Leu
215 220 225gag cca taaaccctaa attctaggaa cga 816Glu Pro230<210>414<211>231<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>414Val Arg His Asp Glu His Tyr Pro Ala Ala Ala Asn Leu Ile Ala Phe1 5 10 15Asp Lys Gly Trp Ser Thr Leu Ile Ala Pro Gln Leu Glu Asp Pro Glu
20 25 30Ala Glu Glu Phe Thr Ala Gly Phe Leu Thr Glu Tyr Gln Asp Asn Leu
35 40 45Ile Thr Ala Gly Met Glu His Gln Ala Leu Ala Ser Gly Phe Pro Val
50 55 60Gly Arg Arg Phe Lys Ser Asp Ile Ala Leu Arg Arg Cys Asp Ala Val65 70 75 80Thr Thr His Ile Gly His Glu His Ser Ala Asp Gly His Trp Arg Ile
85 90 95Tyr Val Phe Ala Gly Gln Ala Thr Pro Gln Asp Ser Glu Ser Ala Leu
100 105 110Asn Lys Trp Ala Gln Trp Met Glu Glu Ser Glu Asp Ser Pro Leu Asn
115 120 125Arg Phe Thr Pro Glu Ala Gly Asp Arg Asn Ala Val Phe Asp Ile Lys
130 135 140Ala Thr Tyr Gln Gln His Tyr His Ser Phe Asp Leu Phe Asp Ala Pro145 150 155 160Glu Val Phe Phe Pro Arg Val Gly Pro Tyr Lys Leu Gln Asn Leu Glu
165 170 175Asn Val Trp Thr Ala Leu Asp Ser Gln Asp Ile Phe Glu Ser Arg Gly
180 185 190Ile Ser Arg Asp Gly Ala Ile Val Val Val Arg Pro Asp Gln Tyr Val
195 200 205Ala Ala Val Leu Pro Leu Glu Asp Thr Ala Ala Leu Ala Glu Phe Phe
210 215 220Asn Gly Asn Leu Leu Glu Pro225 230<210>415<211>1008<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(985)<223>RXN00178<400>415gcgaataatc cacggacggt cacagaagaa aacctcactg cgctgcttac cacagcgctc 60aacggcgacg atccagcaac tttgaattaa ggagaccaac atg act att tca gca 115
Met Thr Ile Ser Ala
1 5caa cag caa gca gtg gaa gaa gac ctt gta gag cgc gta ctc gca tct 163Gln Gln Gln Ala Val Glu Glu Asp Leu Val Glu Arg Val Leu Ala Ser
10 15 20ttt gat tcg tgt gaa aac cct cgc ctc aaa cta gtg atg aaa tcc ctg 211Phe Asp Ser Cys Glu Asn Pro Arg Leu Lys Leu Val Met Lys Ser Leu
25 30 35act gtg cat ctc cat gat ttc atc cgc gat gtt cga ctc act gaa gaa 259Thr Val His Leu His Asp Phe Ile Arg Asp Val Arg Leu Thr Glu Glu
40 45 50gag tgg aac tac gcc att gat ttc ctc acc aag gtt ggg cat atc acc 307Glu Trp Asn Tyr Ala Ile Asp Phe Leu Thr Lys Val Gly His Ile Thr
55 60 65gac gat aag cgc caa gaa ttc gtg ttg ctc tct gac acc ttg ggt gca 355Asp Asp Lys Arg Gln Glu Phe Val Leu Leu Ser Asp Thr Leu Gly Ala70 75 80 85tcc atg cag acc atc gct gtt aat aac gaa gca tat gaa gac gct acc 403Ser Met Gln Thr Ile Ala Val Asn Asn Glu Ala Tyr Glu Asp Ala Thr
90 95 100gaa gca aca gtc ttt ggc ccc ttc ttt gtc gat gat gcg cca ctg gtc 451Glu Ala Thr Val Phe Gly Pro Phe Phe Val Asp Asp Ala Pro Leu Val
105 110 115caa aac gga gat gac att gcc ttt ggc gca gtc ggc cag ccg gca tgg 499Gln Asn Gly Asp Asp Ile Ala Phe Gly Ala Val Gly Gln Pro Ala Trp
120 125 130gtg gag gga acg gtc aaa gac act gaa gga aac ccc att ccc aat gca 547Val Glu Gly Thr Val Lys Asp Thr Glu Gly Asn Pro Ile Pro Asn Ala
135 140 145cgc att gaa gta tgg gaa tgc gat gaa gat gga ctt tat gat gtg caa 595Arg Ile Glu Val Trp Glu Cys Asp Glu Asp Gly Leu Tyr Asp Val Gln150 155 160 165tac gcc gat gag cgc agt gct gga cgc gca cac ctg tat tca gat gaa 643Tyr Ala Asp Glu Arg Ser Ala Gly Arg Ala His Leu Tyr Ser Asp Glu
170 175 180aac ggc gaa tac cac ttc tgg gga cta act ccc gtg cca tat ccc atc 691Asn Gly Glu Tyr His Phe Trp Gly Leu Thr Pro Val Pro Tyr Pro Ile
185 190 195cca cac gat ggt cca gta gga caa atg ctc caa gca gtt ggt cgt tcc 739Pro His Asp Gly Pro Val Gly Gln Met Leu Gln Ala Val Gly Arg Ser
200 205 210ccc gtt cgt tgc gcg cac cta cac ttc atg gtg act gcg cca gag aag 787Pro Val Arg Cys Ala His Leu His Phe Met Val Thr Ala Pro Glu Lys
215 220 225cga acc ttg gta acc cat atc ttc gtt gag ggc gat ccg cag cta gag 835Arg Thr Leu Val Thr His Ile Phe Val Glu Gly Asp Pro Gln Leu Glu230 235 240 245atc ggc gat tcc gtg ttt ggc gtg aag gac tca ctg att aag aaa ttc 883Ile Gly Asp Ser Val Phe Gly Val Lys Asp Ser Leu Ile Lys Lys Phe
250 255 260gtt gag caa cct gca gga acc gca act cca gat ggt cgc gat gtg ggt 931Val Glu Gln Pro Ala Gly Thr Ala Thr Pro Asp Gly Arg Asp Val Gly
265 270 275gat caa acc tgg gca cgc aca cgt ttt gat att gtg ctc gcc ccc ggc 979Asp Gln Thr Trp Ala Arg Thr Arg Phe Asp Ile Val Leu Ala Pro Gly
280 285 290aat gtc taagtagaag cagcaaaaaa cca 1008Asn Val
295<210>416<211>295<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>416Met Thr Ile Ser Ala Gln Gln Gln Ala Val Glu Glu Asp Leu Val Glu1 5 10 15Arg Val Leu Ala Ser Phe Asp Ser Cys Glu Asn Pro Arg Leu Lys Leu
20 25 30Val Met Lys Ser Leu Thr Val His Leu His Asp Phe Ile Arg Asp Val
35 40 45Arg Leu Thr Glu Glu Glu Trp Asn Tyr Ala Ile Asp Phe Leu Thr Lys
50 55 60Val Gly His Ile Thr Asp Asp Lys Arg Gln Glu Phe Val Leu Leu Ser65 70 75 80Asp Thr Leu Gly Ala Ser Met Gln Thr Ile Ala Val Asn Asn Glu Ala
85 90 95Tyr Glu Asp Ala Thr Glu Ala Thr Val Phe Gly Pro Phe Phe Val Asp
100 105 110Asp Ala Pro Leu Val Gln Asn Gly Asp Asp Ile Ala Phe Gly Ala Val
115 120 125Gly Gln Pro Ala Trp Val Glu Gly Thr Val Lys Asp Thr Glu Gly Asn
130 135 140Pro Ile Pro Asn Ala Arg Ile Glu Val Trp Glu Cys Asp Glu Asp Gly145 150 155 160Leu Tyr Asp Val Gln Tyr Ala Asp Glu Arg Ser Ala Gly Arg Ala His
165 170 175Leu Tyr Ser Asp Glu Asn Gly Glu Tyr His Phe Trp Gly Leu Thr Pro
180 185 190Val Pro Tyr Pro Ile Pro His Asp Gly Pro Val Gly Gln Met Leu Gln
195 200 205Ala Val Gly Arg Ser Pro Val Arg Cys Ala His Leu His Phe Met Val
210 215 220Thr Ala Pro Glu Lys Arg Thr Leu Val Thr His Ile Phe Val Glu Gly225 230 235 240Asp Pro Gln Leu Glu Ile Gly Asp Ser Val Phe Gly Val Lys Asp Ser
245 250 255Leu Ile Lys Lys Phe Val Glu Gln Pro Ala Gly Thr Ala Thr Pro Asp
260 265 270Gly Arg Asp Val Gly Asp Gln Thr Trp Ala Arg Thr Arg Phe Asp Ile
275 280 285Val Leu Ala Pro Gly Asn Val
290 295<210>417<211>735<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(712)<223>RXN01461<400>417tcgactatga cgagacccgt gaaaacttcg cgcttggtta caagttcgac atcgtccttc 60gtggccgcaa cgccacccca tttgagtaaa gggttttgca atg att gat aca ggg 115
Met Ile Asp Thr Gly
1 5aag aac ggc gag ttc cgc tac gag cag tcg aat atc atc gat cag aac 163Lys Asn Gly Glu Phe Arg Tyr Glu Gln Ser Asn Ile Ile Asp Gln Asn
10 15 20gaa gcc gag ttc ggc atc act cct tca cag acc gtg ggc cct tac gtc 211Glu Ala Glu Phe Gly Ile Thr Pro Ser Gln Thr Val Gly Pro Tyr Val
25 30 35cac atc ggt ttg acc ctt gaa ggt gcg gag cat ctc gtg gag cca ggt 259His Ile Gly Leu Thr Leu Glu Gly Ala Glu His Leu Val Glu Pro Gly
40 45 50tcg gaa ggc gcg gtg tcc ttt act gtt tcc gca act gat ggc aac ggc 307Ser Glu Gly Ala Val Ser Phe Thr Val Ser Ala Thr Asp Gly Asn Gly
55 60 65gac ccc atc gcg gat gcc atg ttt gaa ctg tgg cag gcc gat cca gag 355Asp Pro Ile Ala Asp Ala Met Phe Glu Leu Trp Gln Ala Asp Pro Glu70 75 80 85ggc atc cac aac tct gat ttg gat cca aac cgc aca gca cca gca acc 403Gly Ile His Asn Ser Asp Leu Asp Pro Asn Arg Thr Ala Pro Ala Thr
90 95 100gca gat ggc ttc cgc ggg ctt ggt cgc gcg atg gca aac gcg cag ggt 451Ala Asp Gly Phe Arg Gly Leu Gly Arg Ala Met Ala Asn Ala Gln Gly
105 110 115gag gca acg ttc acc act ttg gtt ccg gga gca ttc gca gat gag gca 499Glu Ala Thr Phe Thr Thr Leu Val Pro Gly Ala Phe Ala Asp Glu Ala
120 125 130cca cac ttc aag gtt ggt gtg ttc gcc cgt ggc atg ctg gag cgt ctg 547Pro His Phe Lys Val Gly Val Phe Ala Arg Gly Met Leu Glu Arg Leu
135 140 145tac act cgc gca tac ctg cca gac gcc gat ttg agc acc gac cca gtt 595Tyr Thr Arg Ala Tyr Leu Pro Asp Ala Asp Leu Ser Thr Asp Pro Val150 155 160 165ttg gct gtg gtc cca gct gat cga cgt gac ctc ctg gtg gct caa aag 643Leu Ala Val Val Pro Ala Asp Arg Arg Asp Leu Leu Val Ala Gln Lys
170 175 180acc gat gat gga ttc cgc ttc gac atc act gtc cag gct gaa gac aat 691Thr Asp Asp Gly Phe Arg Phe Asp Ile Thr Val Gln Ala Glu Asp Asn
185 190 195gaa acc cca ttt ttt gga ctc taaattgacc cgatctttat act 735Glu Thr Pro Phe Phe Gly Leu
200<210>418<211>204<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>418Met Ile Asp Thr Gly Lys Asn Gly Glu Phe Arg Tyr Glu Gln Ser Asn1 5 10 15Ile Ile Asp Gln Asn Glu Ala Glu Phe Gly Ile Thr Pro Ser Gln Thr
20 25 30Val Gly Pro Tyr Val His Ile Gly Leu Thr Leu Glu Gly Ala Glu His
35 40 45Leu Val Glu Pro Gly Ser Glu Gly Ala Val Ser Phe Thr Val Ser Ala
50 55 60Thr Asp Gly Asn Gly Asp Pro Ile Ala Asp Ala Met Phe Glu Leu Trp65 70 75 80Gln Ala Asp Pro Glu Gly Ile His Asn Ser Asp Leu Asp Pro Asn Arg
85 90 95Thr Ala Pro Ala Thr Ala Asp Gly Phe Arg Gly Leu Gly Arg Ala Met
100 105 110Ala Asn Ala Gln Gly Glu Ala Thr Phe Thr Thr Leu Val Pro Gly Ala
115 120 125Phe Ala Asp Glu Ala Pro His Phe Lys Val Gly Val Phe Ala Arg Gly
130 135 140Met Leu Glu Arg Leu Tyr Thr Arg Ala Tyr Leu Pro Asp Ala Asp Leu145 150 155 160Ser Thr Asp Pro Val Leu Ala Val Val Pro Ala Asp Arg Arg Asp Leu
165 170 175Leu Val Ala Gln Lys Thr Asp Asp Gly Phe Arg Phe Asp Ile Thr Val
180 185 190Gln Ala Glu Asp Asn Glu Thr Pro Phe Phe Gly Leu
195 200<210>419<211>1584<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1561)<223>RXN01653<400>419ttcattcagg gtgaatgctc tccttgtttc agatgttcaa cgctccataa agtagaccgc 60aatctagaca aagatgtcta ttttaattaa ggagcagaac atg gcc acg gcc gag 115
Met Ala Thr Ala Glu
1 5aac aca aca cag gag aat cgg aaa atc ctg ttc aac gca ttt gat atg 163Asn Thr Thr Gln Glu Asn Arg Lys Ile Leu Phe Asn Ala Phe Asp Met
10 15 20aac tgc gtt gcg cat cag tcc cca gga ctg tgg aca cac ccg aag gat 211Asn Cys Val Ala His Gln Ser Pro Gly Leu Trp Thr His Pro Lys Asp
25 30 35aag gcg cga gac tac aac act ctt gat tac tgg gtg cac ctt gcc aag 259Lys Ala Arg Asp Tyr Asn Thr Leu Asp Tyr Trp Val His Leu Ala Lys
40 45 50act ttg gag aag ggc ctt ttc gac ggc ctt ttc atc gca gat gtg ctt 307Thr Leu Glu Lys Gly Leu Phe Asp Gly Leu Phe Ile Ala Asp Val Leu
55 60 65gga act tac gat gtt tat ggt tct agt aat gaa gcg gcg ttg agc agt 355Gly Thr Tyr Asp Val Tyr Gly Ser Ser Asn Glu Ala Ala Leu Ser Ser70 75 80 85ggt gcg cag gtg cct gtc aat gat ccg atc ctt ctt gtt tct gcg atg 403Gly Ala Gln Val Pro Val Asn Asp Pro Ile Leu Leu Val Ser Ala Met
90 95 100gcc tat gcc aca aag aac ctc ggg ttt ggc att act gca ggt act gcc 451Ala Tyr Ala Thr Lys Asn Leu Gly Phe Gly Ile Thr Ala Gly Thr Ala
105 110 115tat gag cac ccg tat cct ttt gcg cgg cgt ctg gcc aca ctt gat cac 499Tyr Glu His Pro Tyr Pro Phe Ala Arg Arg Leu Ala Thr Leu Asp His
120 125 130ctg act aat ggg cgt gtg ggg tgg aat gtg gtt act ggc tat ctt ccc 547Leu Thr Asn Gly Arg Val Gly Trp Asn Val Val Thr Gly Tyr Leu Pro
135 140 145tct gct gct caa aac atg ggt gac acc gat cag ctg cca cat gat gag 595Ser Ala Ala Gln Asn Met Gly Asp Thr Asp Gln Leu Pro His Asp Glu150 155 160 165cgc tat gac aaa gca gat gaa tac ctg gaa gtg atc tac aag ctt ctc 643Arg Tyr Asp Lys Ala Asp Glu Tyr Leu Glu Val Ile Tyr Lys Leu Leu
170 175 180gag ggc tcc tgg gaa gac gat gct gtt caa aac aat acg gag acg agt 691Glu Gly Ser Trp Glu Asp Asp Ala Val Gln Asn Asn Thr Glu Thr Ser
185 190 195gtc ttt acg gac tcc tcc aaa gtg cac gcc att aat cat cat ggc aag 739Val Phe Thr Asp Ser Ser Lys Val His Ala Ile Asn His His Gly Lys
200 205 210tac ttt gat gtg ccg ggc att gcc atc act gag ccg agt gtg cag cgt 787Tyr Phe Asp Val Pro Gly Ile Ala Ile Thr Glu Pro Ser Val Gln Arg
215 220 225acg ccg gtg atc tac cag gcg ggt gca tcg ccg cgc gga ttg aaa ttc 835Thr Pro Val Ile Tyr Gln Ala Gly Ala Ser Pro Arg Gly Leu Lys Phe230 235 240 245gct ggt gag aat gca gaa gca gtg ttt atc aat tcc agc acc gtg gag 883Ala Gly Glu Asn Ala Glu Ala Val Phe Ile Asn Ser Ser Thr Val Glu
250 255 260gca atc acc aag act gtc gca aaa att cgc gct gct gcg gtc gct gcg 931Ala Ile Thr Lys Thr Val Ala Lys Ile Arg Ala Ala Ala Val Ala Ala
265 270 275gga cgt gat cca cat gcg gtg aag atc ttt gcg atg caa acc atc atc 979Gly Arg Asp Pro His Ala Val Lys Ile Phe Ala Met Gln Thr Ile Ile
280 285 290act ggt gaa aca gaa gca gat gcg cag gca aag ctg gag gaa tac agt 1027Thr Gly Glu Thr Glu Ala Asp Ala Gln Ala Lys Leu Glu Glu Tyr Ser
295 300 305cgc tat atc gat cct gtc ggt ggt ctg acc ttg atg tct gga tgg acc 1075Arg Tyr Ile Asp Pro Val Gly Gly Leu Thr Leu Met Ser Gly Trp Thr310 315 320 325ggc gcg gat ctg tcg cag tat gac ctg gat gaa ccg atc acc aat att 1123Gly Ala Asp Leu Ser Gln Tyr Asp Leu Asp Glu Pro Ile Thr Asn Ile
330 335 340gag tca aac gct att cag tcc act gca gcc acc att agc aac ggc acc 1171Glu Ser Asn Ala Ile Gln Ser Thr Ala Ala Thr Ile Ser Asn Gly Thr
345 350 355ggt gaa ggt gcg tgg acg gta cgc aaa ctg ggt gag gca acc ggc atc 1219Gly Glu Gly Ala Trp Thr Val Arg Lys Leu Gly Glu Ala Thr Gly Ile
360 365 370ggc ggc ttc gga cca gtg ctt gtg gga tct ggc gct aac gtt gcc gcg 1267Gly Gly Phe Gly Pro Val Leu Val Gly Ser Gly Ala Asn Val Ala Ala
375 380 385gaa ctt gca cgc atc cag gat ctc agc gat gtt gat ggt ttc aac ctt 1315Glu Leu Ala Arg Ile Gln Asp Leu Ser Asp Val Asp Gly Phe Asn Leu390 395 400 405gct tat gcc atc acc cca gga act ttt gaa gat gtc gtg gac ttt gtg 1363Ala Tyr Ala Ile Thr Pro Gly Thr Phe Glu Asp Val Val Asp Phe Val
410 415 420gtg cct gag ctg caa aaa ctt agc cgc tac aag acg gaa tac gcg ccg 1411Val Pro Glu Leu Gln Lys Leu Ser Arg Tyr Lys Thr Glu Tyr Ala Pro
425 430 435ggt tcc ttg cgc aac aaa ttg ctc ggt aaa ggt gat cgc ctg gac gat 1459Gly Ser Leu Arg Asn Lys Leu Leu Gly Lys Gly Asp Arg Leu Asp Asp
440 445 450acc cac cgc ggc gca agc tac cgc cta ggc gct cgg aac tcc acc gcc 1507Thr His Arg Gly Ala Ser Tyr Arg Leu Gly Ala Arg Asn Ser Thr Ala
455 460 465act att gat ctc agt tcc ata tcc gcc caa cta gtt tcc cag gga gcc 1555Thr Ile Asp Leu Ser Ser Ile Ser Ala Gln Leu Val Ser Gln Gly Ala470 475 480 485cac tca tgatctcacc gcaaacaatc atc 1584His Ser<210>420<211>487<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>420Met Ala Thr Ala Glu Asn Thr Thr Gln Glu Asn Arg Lys Ile Leu Phe1 5 10 15Asn Ala Phe Asp Met Asn Cys Val Ala His Gln Ser Pro Gly Leu Trp
20 25 30Thr His Pro Lys Asp Lys Ala Arg Asp Tyr Asn Thr Leu Asp Tyr Trp
35 40 45Val His Leu Ala Lys Thr Leu Glu Lys Gly Leu Phe Asp Gly Leu Phe
50 55 60Ile Ala Asp Val Leu Gly Thr Tyr Asp Val Tyr Gly Ser Ser Asn Glu65 70 75 80Ala Ala Leu Ser Ser Gly Ala Gln Val Pro Val Asn Asp Pro Ile Leu
85 90 95Leu Val Ser Ala Met Ala Tyr Ala Thr Lys Asn Leu Gly Phe Gly Ile
100 105 110Thr Ala Gly Thr Ala Tyr Glu His Pro Tyr Pro Phe Ala Arg Arg Leu
115 120 125Ala Thr Leu Asp His Leu Thr Asn Gly Arg Val Gly Trp Asn Val Val
130 135 140Thr Gly Tyr Leu Pro Ser Ala Ala Gln Asn Met Gly Asp Thr Asp Gln145 150 155 160Leu Pro His Asp Glu Arg Tyr Asp Lys Ala Asp Glu Tyr Leu Glu Val
165 170 175Ile Tyr Lys Leu Leu Glu Gly Ser Trp Glu Asp Asp Ala Val Gln Asn
180 185 190Asn Thr Glu Thr Ser Val Phe Thr Asp Ser Ser Lys Val His Ala Ile
195 200 205Asn His His Gly Lys Tyr Phe Asp Val Pro Gly Ile Ala Ile Thr Glu
210 215 220Pro Ser Val Gln Arg Thr Pro Val Ile Tyr Gln Ala Gly Ala Ser Pro225 230 235 240Arg Gly Leu Lys Phe Ala Gly Glu Asn Ala Glu Ala Val Phe Ile Asn
245 250 255Ser Ser Thr Val Glu Ala Ile Thr Lys Thr Val Ala Lys Ile Arg Ala
260 265 270Ala Ala Val Ala Ala Gly Arg Asp Pro His Ala Val Lys Ile Phe Ala
275 280 285Met Gln Thr Ile Ile Thr Gly Glu Thr Glu Ala Asp Ala Gln Ala Lys
290 295 300Leu Glu Glu Tyr Ser Arg Tyr Ile Asp Pro Val Gly Gly Leu Thr Leu305 310 315 320Met Ser Gly Trp Thr Gly Ala Asp Leu Ser Gln Tyr Asp Leu Asp Glu
325 330 335Pro Ile Thr Asn Ile Glu Ser Asn Ala Ile Gln Ser Thr Ala Ala Thr
340 345 350Ile Ser Asn Gly Thr Gly Glu Gly Ala Trp Thr Val Arg Lys Leu Gly
355 360 365Glu Ala Thr Gly Ile Gly Gly Phe Gly Pro Val Leu Val Gly Ser Gly
370 375 380Ala Asn Val Ala Ala Glu Leu Ala Arg Ile Gln Asp Leu Ser Asp Val385 390 395 400Asp Gly Phe Asn Leu Ala Tyr Ala Ile Thr Pro Gly Thr Phe Glu Asp
405 410 415Val Val Asp Phe Val Val Pro Glu Leu Gln Lys Leu Ser Arg Tyr Lys
420 425 430Thr Glu Tyr Ala Pro Gly Ser Leu Arg Asn Lys Leu Leu Gly Lys Gly
435 440 445Asp Arg Leu Asp Asp Thr His Arg Gly Ala Ser Tyr Arg Leu Gly Ala
450 455 460Arg Asn Ser Thr Ala Thr Ile Asp Leu Ser Ser Ile Ser Ala Gln Leu465 470 475 480Val Ser Gln Gly Ala His Ser
485<210>421<211>702<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(679)<223>RXN02053<400>421aaccagccag aaactatctc caaaagctaa taaaaccctt gcactgacaa ataaggcgac 60ctaccatgac tctgtttcca acacataaaa aggataaaaa atg tca ctt tca gtc 115
Met Ser Leu Ser Val
1 5gtc gag gcg att acc aac cgc cgc gcc acc cgc aaa tac acc gat gaa 163Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg Lys Tyr Thr Asp Glu
10 15 20gct cct acc cct gag ctg atc gac aaa atc gtt gac ctt gcc ctg gag 211Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val Asp Leu Ala Leu Glu
25 30 35gca ccc agt gcg ttc aat gcg cag caa cgt gaa att gtt gtg att act 259Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu Ile Val Val Ile Thr
40 45 50gat ccc gca cag aag cag aag ctt tac gag gcc tcc cat cag aaa caa 307Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala Ser His Gln Lys Gln
55 60 65ttc ctc acc gca cct gta act ttc att gcg gtt gcc cgc gtg gaa aac 355Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val Ala Arg Val Glu Asn70 75 80 85gag cct gag gat ttg gaa gag att ctt ggt acg gaa agg gct gaa cgt 403Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr Glu Arg Ala Glu Arg
90 95 100gtc gcg gga ttc atc aac ggt cgc agc att cag cag gca cgc gaa gca 451Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln Gln Ala Arg Glu Ala
105 110 115acg ttg agg gat gcc agc ctc gcg gcg gct ttt cta att ctg gct gcc 499Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe Leu Ile Leu Ala Ala
120 125 130cag gcg gag ggt ttg agt acc agc ccg act act ggt tgg gat gag gaa 547Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr Gly Trp Asp Glu Glu
135 140 145aaa gtg aag gaa gca atc ggt ctc ggc ggg cgt gag gat cgt gca atc 595Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg Glu Asp Arg Ala Ile150 155 160 165gcc ctt gtt att gct acc gga ttc cct aat gaa cag ccg gag cac cct 643Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu Gln Pro Glu His Pro
170 175 180ggt cgt ttg cag aat agg cgc atc gac aac agc tac taactctgcc 689Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser Tyr
185 190agctcgcccg gac 702<210>422<211>193<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>422Met Ser Leu Ser Val Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg1 5 10 15Lys Tyr Thr Asp Glu Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val
20 25 30Asp Leu Ala Leu Glu Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu
35 40 45Ile Val Val Ile Thr Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala
50 55 60Ser His Gln Lys Gln Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val65 70 75 80Ala Arg Val Glu Asn Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr
85 90 95Glu Arg Ala Glu Arg Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln
100 105 110Gln Ala Arg Glu Ala Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe
115 120 125Leu Ile Leu Ala Ala Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr
130 l35 140Gly Trp Asp Glu Glu Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg145 150 155 160Glu Asp Arg Ala Ile Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu
165 170 175Gln Pro Glu His Pro Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser
180 185 190Tyr<210>423<211>1191<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1168)<223>RXN00177<400>423ctattccaca gcagtggacg ctgaacaact tcaaacacag attaagcagc tatcggatct 60acttcacctc aactcagttg tcggagcata ggagctaaaa atg tct tta cag ttc 115
Met Ser Leu Gln Phe
1 5gat cat gaa acc ctc ggt caa cga gtt ctg ttc ggt tca ggt gag gcg 163Asp His Glu Thr Leu Gly Gln Arg Val Leu Phe Gly Ser Gly Glu Ala
10 15 20gcg caa aat ctc gcc gct gaa att agc cga ctc gat gcc aaa aac gtc 211Ala Gln Asn Leu Ala Ala Glu Ile Ser Arg Leu Asp Ala Lys Asn Val
25 30 35atg gtg gtt gcc ggt gat ttc gag ctt ccc atg gca cgg caa gta gca 259Met Val Val Ala Gly Asp Phe Glu Leu Pro Met Ala Arg Gln Val Ala
40 45 50gca gat att gat gtc aag gtg tgg cat tca aat gtc gtg atg cat gtg 307Ala Asp Ile Asp Val Lys Val Trp His Ser Asn Val Val Met His Val
55 60 65ccc atc gaa aca gca gaa gaa gca cgc agt gtt gcg aaa gaa aac gac 355Pro Ile Glu Thr Ala Glu Glu Ala Arg Ser Val Ala Lys Glu Asn Asp70 75 80 85att gat gtt gtg gtg tgt gtg ggc ggt gga tcc aca aca ggt cta gct 403Ile Asp Val Val Val Cys Val Gly Gly Gly Ser Thr Thr Gly Leu Ala
90 95 100aaa gcg att gcc atg acc acc gca ttg ccg atc att gcg gta ccc act 451Lys Ala Ile Ala Met Thr Thr Ala Leu Pro Ile Ile Ala Val Pro Thr
105 110 115act tat gca ggt tct gaa gca aca aat gtg tgg gga ttg acc gaa gcc 499Thr Tyr Ala Gly Ser Glu Ala Thr Asn Val Trp Gly Leu Thr Glu Ala
120 125 130gcg cgc aaa aca act ggt gtt gat aac aaa gtg ctg cca gtg aca gtt 547Ala Arg Lys Thr Thr Gly Val Asp Asn Lys Val Leu Pro Val Thr Val
135 140 145atc tac gat tca gcg tta acc atg tct ttg ccg gta gaa atg tcg gtt 595Ile Tyr Asp Ser Ala Leu Thr Met Ser Leu Pro Val Glu Met Ser Val150 155 160 165gct tct ggt ctc aat ggt ttg gct cac tgc att gat tct ttg tgg gga 643Ala Ser Gly Leu Asn Gly Leu Ala His Cys Ile Asp Ser Leu Trp Gly
170 175 180ccg aag gcg gat ccc atc aat gcg gct atg gct gct gag gga att cga 691Pro Lys Ala Asp Pro Ile Asn Ala Ala Met Ala Ala Glu Gly Ile Arg
185 190 195gca ctt tct gct ggc ctt ccc aag att gtg gca gat gct cag gac gta 739Ala Leu Ser Ala Gly Leu Pro Lys Ile Val Ala Asp Ala Gln Asp Val
200 205 210gat ggt cgc gat gaa gcg ctc tac ggt gcc tac ctg gct gcg gtg tct 787Asp Gly Arg Asp Glu Ala Leu Tyr Gly Ala Tyr Leu Ala Ala Val Ser
215 220 225ttt gcc tct gct ggc tct ggt ctc cac cac aag atc tgc cac gtg ttg 835Phe Ala Ser Ala Gly Ser Gly Leu His His Lys Ile Cys His Val Leu230 235 240 245ggt gga act ttt aac ctt cca cac gcg caa acc cat gca aca gta ctg 883Gly Gly Thr Phe Asn Leu Pro His Ala Gln Thr His Ala Thr Val Leu
250 255 260cct tat gtt ctt gcc ttc aac gcg cca tat gcg cca cag gca gaa caa 931Pro Tyr Val Leu Ala Phe Asn Ala Pro Tyr Ala Pro Gln Ala Glu Gln
265 270 275cgc gca gcg gca gct ttc ggt tct gcg aca gca ctt gaa gga ttg caa 979Arg Ala Ala Ala Ala Phe Gly Ser Ala Thr Ala Leu Glu Gly Leu Gln
280 285 290cag ctg cgt gcc caa gtg gga gca cca cag cga cta tcc gat tac gga 1027Gln Leu Arg Ala Gln Val Gly Ala Pro Gln Arg Leu Ser Asp Tyr Gly
295 300 305ttc acc gca gca gga atc cca gag gca gtg gaa atc atc ttg gag aaa 1075Phe Thr Ala Ala Gly Ile Pro Glu Ala Val Glu Ile Ile Leu Glu Lys310 315 320 325gta ccg gcg aat aat cca cgg acg gtc aca gaa gaa aac ctc act gcg 1123Val Pro Ala Asn Asn Pro Arg Thr Val Thr Glu Glu Asn Leu Thr Ala
330 335 340ctg ctt acc aca gcg ctc aac ggc gac gat cca gca act ttg aat 1168Leu Leu Thr Thr Ala Leu Asn Gly Asp Asp Pro Ala Thr Leu Asn
345 350 355taaggagacc aacatgacta ttt 1191<210>424<211>356<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>424Met Ser Leu Gln Phe Asp His Glu Thr Leu Gly Gln Arg Val Leu Phe1 5 10 15Gly Ser Gly Glu Ala Ala Gln Asn Leu Ala Ala Glu Ile Ser Arg Leu
20 25 30Asp Ala Lys Asn Val Met Val Val Ala Gly Asp Phe Glu Leu Pro Met
35 40 45Ala Arg Gln Val Ala Ala Asp Ile Asp Val Lys Val Trp His Ser Asn
50 55 60Val Val Met His Val Pro Ile Glu Thr Ala Glu Glu Ala Arg Ser Val65 70 75 80Ala Lys Glu Asn Asp Ile Asp Val Val Val Cys Val Gly Gly Gly Ser
85 90 95Thr Thr Gly Leu Ala Lys Ala Ile Ala Met Thr Thr Ala Leu Pro Ile
100 105 110Ile Ala Val Pro Thr Thr Tyr Ala Gly Ser Glu Ala Thr Asn Val Trp
115 120 125Gly Leu Thr Glu Ala Ala Arg Lys Thr Thr Gly Val Asp Asn Lys Val
130 135 140Leu Pro Val Thr Val Ile Tyr Asp Ser Ala Leu Thr Met Ser Leu Pro145 150 155 160Val Glu Met Ser Val Ala Ser Gly Leu Asn Gly Leu Ala His Cys Ile
165 170 175Asp Ser Leu Trp Gly Pro Lys Ala Asp Pro Ile Asn Ala Ala Met Ala
180 185 190Ala Glu Gly Ile Arg Ala Leu Ser Ala Gly Leu Pro Lys Ile Val Ala
195 200 205Asp Ala Gln Asp Val Asp Gly Arg Asp Glu Ala Leu Tyr Gly Ala Tyr
210 215 220Leu Ala Ala Val Ser Phe Ala Ser Ala Gly Ser Gly Leu His His Lys225 230 235 240Ile Cys His Val Leu Gly Gly Thr Phe Asn Leu Pro His Ala Gln Thr
245 250 255His Ala Thr Val Leu Pro Tyr Val Leu Ala Phe Asn Ala Pro Tyr Ala
260 265 270Pro Gln Ala Glu Gln Arg Ala Ala Ala Ala Phe Gly Ser Ala Thr Ala
275 280 285Leu Glu Gly Leu Gln Gln Leu Arg Ala Gln Val Gly Ala Pro Gln Arg
290 295 300Leu Ser Asp Tyr Gly Phe Thr Ala Ala Gly Ile Pro Glu Ala Val Glu305 310 315 320Ile Ile Leu Glu Lys Val Pro Ala Asn Asn Pro Arg Thr Val Thr Glu
325 330 335Glu Asn Leu Thr Ala Leu Leu Thr Thr Ala Leu Asn Gly Asp Asp Pro
340 345 350Ala Thr Leu Asn
355<210>425<211>960<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(937)<223>RXC00963<400>425ctggctctga cggcgtcgac ttgttctgct tctctgatac accaattttc gaggccctta 60acctcgcacg tacttttact ccggaaggaa tctagaactt atg cgt ctt gca aca 115
Met Arg Leu Ala Thr
1 5atc cgc acc aac ggc acc acc att gct gct cgt gtt gaa tct gaa aac 163Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg Val Glu Ser Glu Asn
10 15 20acc gct acc acc atc gag ggc ttt gcc aac gtc ggt gaa tta ctc cag 211Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val Gly Glu Leu Leu Gln
25 30 35gaa tcc aac tgg cgc gag ctg gca gaa aac gct gct ggt gag gct gtg 259Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala Ala Gly Glu Ala Val
40 45 50acc ttt gaa aac aag gag cta gat gca gta gtt cca gca cct aag aag 307Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val Pro Ala Pro Lys Lys
55 60 65att gtg tgc gtc ggc ctt aac tac gcc aac cac att aaa gaa atg ggc 355Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His Ile Lys Glu Met Gly70 75 80 85cgc gac ctc cct gat acc cca acc ctt ttt gtt aag ttc cct gac gcg 403Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val Lys Phe Pro Asp Ala
90 95 100ctc atc gga cct ttc gat gat gtt gtc gtt cca gag tgg gct aac aag 451Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro Glu Trp Ala Asn Lys
105 110 115gct ctc gac tgg gaa ggc gag atg gca gtt atc att ggc aag cgc gca 499Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile Ile Gly Lys Arg Ala
120 125 130cgc cgt gtc aag cag gcc gat gct gct gag tac atc gct ggc tac gca 547Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr Ile Ala Gly Tyr Ala
135 140 145gtg atg aac gat tac acc acc cgc gat ttc cag tac gca gca cct gca 595Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln Tyr Ala Ala Pro Ala150 155 160 165aag act cca cag tgg cac cag ggc aag tct ttg gaa aag tcc gct ggc 643Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu Glu Lys Ser Ala Gly
170 175 180ttc ggg cct tgg atg act acc cca gat tct ttt gag ttc ggc ggc gag 691Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe Glu Phe Gly Gly Glu
185 190 195ctg gca acc tac ctc gag ggc gag aag gta cag tcc acc cct acc aat 739Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln Ser Thr Pro Thr Asn
200 205 210gac ctg gtc ttt agc cca gaa aag ctc atc gaa tac atc acc cac atc 787Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu Tyr Ile Thr His Ile
215 220 225tac cca ttg gat gct ggc gac gtc att gtc acc ggt acc cca ggc ggc 835Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr Gly Thr Pro Gly Gly230 235 240 245gtt ggc cac gca cgt aac cca cag cgc tac atc ggt gac ggc gaa acc 883Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile Gly Asp Gly Glu Thr
250 255 260gta aag gtt gag att gcg ggc ctc ggc ttc att gaa aac aag acg gtg 931Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile Glu Asn Lys Thr Val
265 270 275ttt gaa taaatgacaa ctttccacga tct 960Phe Glu<210>426<211>279<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>426Met Arg Leu Ala Thr Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg1 5 10 15Val Glu Ser Glu Asn Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val
20 25 30Gly Glu Leu Leu Gln Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala
35 40 45Ala Gly Glu Ala Val Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val
50 55 60Pro Ala Pro Lys Lys Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His65 70 75 80Ile Lys Glu Met Gly Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val
85 90 95Lys Phe Pro Asp Ala Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro
100 105 110Glu Trp Ala Asn Lys Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile
115 120 125Ile Gly Lys Arg Ala Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr
130 135 140Ile Ala Gly Tyr Ala Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln145 150 155 160Tyr Ala Ala Pro Ala Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu
165 170 175Glu Lys Ser Ala Gly Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe
180 185 190Glu Phe Gly Gly Glu Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln
195 200 205Ser Thr Pro Thr Asn Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu
210 215 220Tyr Ile Thr His Ile Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr225 230 235 240Gly Thr Pro Gly Gly Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile
245 250 255Gly Asp Gly Glu Thr Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile
260 265 270Glu Asn Lys Thr Val Phe Glu
275<210>427<211>1101<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1078)<223>RXN00299<400>427tgccatcggt ttggctattg attggaacaa gaaaggtgcc cagtctgttg caaagaagga 60atccatttcc gtctaatcgc taattgcgag gagtctttgc atg tct atc cca ctt 115
Met Ser Ile Pro Leu
1 5tca ctg att gat ttt gcc acc att ttt gag ggc gaa agg cct ggt gac 163Ser Leu Ile Asp Phe Ala Thr Ile Phe Glu Gly Glu Arg Pro Gly Asp
10 15 20agc ttc aaa cga tca gtg gca ttg gcg caa aaa gct gaa ggt tta ggc 211Ser Phe Lys Arg Ser Val Ala Leu Ala Gln Lys Ala Glu Gly Leu Gly
25 30 35ttc aag cgc att tgg tac gca gag cat cac aac atg gag agc att tct 259Phe Lys Arg Ile Trp Tyr Ala Glu His His Asn Met Glu Ser Ile Ser
40 45 50tca gct gct cct gca gtg ctt att tct cac atc ggt gcg aac acc aag 307Ser Ala Ala Pro Ala Val Leu Ile Ser His Ile Gly Ala Asn Thr Lys
55 60 65act att cgt ctg ggt gcc ggc ggc gtc atg ctg ccc aac cac tcc cca 355Thr Ile Arg Leu Gly Ala Gly Gly Val Met Leu Pro Asn His Ser Pro70 75 80 85tat gtc atc gct gag cag ttc ggc acc ttg gcg gag ttg tac cca gac 403Tyr Val Ile Ala Glu Gln Phe Gly Thr Leu Ala Glu Leu Tyr Pro Asp
90 95 100cgc atc gac ctc ggc ctg ggc cgt gcc cct ggc acg gac atg aat acc 451Arg Ile Asp Leu Gly Leu Gly Arg Ala Pro Gly Thr Asp Met Asn Thr
105 110 115ttg cgc gct tta cga cgc gac cct cag tcc gcc gag aac ttc ccg tcc 499Leu Arg Ala Leu Arg Arg Asp Pro Gln Ser Ala Glu Asn Phe Pro Ser
120 125 130gac gtt gtc gag ctg aac tct tac ctc acc ggc cgt tcc cgt ctc cca 547Asp Val Val Glu Leu Asn Ser Tyr Leu Thr Gly Arg Ser Arg Leu Pro
135 140 145ggg gtt aac gca att cca ggc aag ggc acc aac gta ccg ctg tac atc 595Gly Val Asn Ala Ile Pro Gly Lys Gly Thr Asn Val Pro Leu Tyr Ile150 155 160 165ttg ggt tca tcc ctc ttt ggt gca caa ttg gca gca cag ttg ggt atg 643Leu Gly Ser Ser Leu Phe Gly Ala Gln Leu Ala Ala Gln Leu Gly Met
170 175 180cct tat tcc ttc gca tcc cac ttc gca cca act cac ctt gag cac gcg 691Pro Tyr Ser Phe Ala Ser His Phe Ala Pro Thr His Leu Glu His Ala
185 190 195gtg caa acc tac cgg gat aac tac cag cct tca gag cag cat cct gag 739Val Gln Thr Tyr Arg Asp Asn Tyr Gln Pro Ser Glu Gln His Pro Glu
200 205 210cct tat gtc att gcg gcc gtc aat gtc acc gca tct gat tcc act gaa 787Pro Tyr Val Ile Ala Ala Val Asn Val Thr Ala Ser Asp Ser Thr Glu
215 220 225caa gcc cac gat gat ttc tac aag gta gcg cgt gca cgc gtg aag aac 835Gln Ala His Asp Asp Phe Tyr Lys Val Ala Arg Ala Arg Val Lys Asn230 235 240 245atg gca ttg cgt ggc cga caa gtt act gat gag caa ctt gat gaa ctc 883Met Ala Leu Arg Gly Arg Gln Val Thr Asp Glu Gln Leu Asp Glu Leu
250 255 260atg gat tca cca gct gct cgc caa att gtc gac atg ctt cac tac acc 931Met Asp Ser Pro Ala Ala Arg Gln Ile Val Asp Met Leu His Tyr Thr
265 270 275gct ata ggc act gga tcc gaa gtt aaa gaa tac cta gac ggt ttt gta 979Ala Ile Gly Thr Gly Ser Glu Val Lys Glu Tyr Leu Asp Gly Phe Val
280 285 290aag acg gca cag gct gat gaa ctg atg atc tcc ctg caa tcc ccc aac 1027Lys Thr Ala Gln Ala Asp Glu Leu Met Ile Ser Leu Gln Ser Pro Asn
295 300 305act gaa gca acc acg cgc aat atg gaa att ctt gcg gat gcg tgg att 1075Thr Glu Ala Thr Thr Arg Asn Met Glu Ile Leu Ala Asp Ala Trp Ile310 315 320 325aat tagtaccgat gggccggtag aca 1101Asn<210>428<211>326<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>428Met Ser Ile Pro Leu Ser Leu Ile Asp Phe Ala Thr Ile Phe Glu Gly1 5 10 15Glu Arg Pro Gly Asp Ser Phe Lys Arg Ser Val Ala Leu Ala Gln Lys
20 25 30Ala Glu Gly Leu Gly Phe Lys Arg Ile Trp Tyr Ala Glu His His Asn
35 40 45Met Glu Ser Ile Ser Ser Ala Ala Pro Ala Val Leu Ile Ser His Ile
50 55 60Gly Ala Asn Thr Lys Thr Ile Arg Leu Gly Ala Gly Gly Val Met Leu65 70 75 80Pro Asn His Ser Pro Tyr Val Ile Ala Glu Gln Phe Gly Thr Leu Ala
85 90 95Glu Leu Tyr Pro Asp Arg Ile Asp Leu Gly Leu Gly Arg Ala Pro Gly
100 105 110Thr Asp Met Asn Thr Leu Arg Ala Leu Arg Arg Asp Pro Gln Ser Ala
115 120 125Glu Asn Phe Pro Ser Asp Val Val Glu Leu Asn Ser Tyr Leu Thr Gly
130 135 140Arg Ser Arg Leu Pro Gly Val Asn Ala Ile Pro Gly Lys Gly Thr Asn145 150 155 160Val Pro Leu Tyr Ile Leu Gly Ser Ser Leu Phe Gly Ala Gln Leu Ala
165 170 175Ala Gln Leu Gly Met Pro Tyr Ser Phe Ala Ser His Phe Ala Pro Thr
180 185 190His Leu Glu His Ala Val Gln Thr Tyr Arg Asp Asn Tyr Gln Pro Ser
195 200 205Glu Gln His Pro Glu Pro Tyr Val Ile Ala Ala Val Asn Val Thr Ala
210 215 220Ser Asp Ser Thr Glu Gln Ala His Asp Asp Phe Tyr Lys Val Ala Arg225 230 235 240Ala Arg Val Lys Asn Met Ala Leu Arg Gly Arg Gln Val Thr Asp Glu
245 250 255Gln Leu Asp Glu Leu Met Asp Ser Pro Ala Ala Arg Gln Ile Val Asp
260 265 270Met Leu His Tyr Thr Ala Ile Gly Thr Gly Ser Glu Val Lys Glu Tyr
275 280 285Leu Asp Gly Phe Val Lys Thr Ala Gln Ala Asp Glu Leu Met Ile Ser
290 295 300Leu Gln Ser Pro Asn Thr Glu Ala Thr Thr Arg Asn Met Glu Ile Leu305 310 315 320Ala Asp Ala Trp Ile Asn
325<210>429<21l>784<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(18)..(761)<223>FRXA00299<400>429gggtgccggc ggcggtc atg ctg ccc aac cac tcc cca tat gtc atc gct gag 53
Met Leu Pro Asn His Ser Pro Tyr Val Ile Ala Glu
1 5 10cag ttc ggc acc ttg gcg gag ttg tac cca gac cgc atc gac ctc ggc 101Gln Phe Gly Thr Leu Ala Glu Leu Tyr Pro Asp Arg Ile Asp Leu Gly
15 20 25atg ggc cgt gcc cct ggc acg gac atg aat acc ttg cgc gct tta cga 149Met Gly Arg Ala Pro Gly Thr Asp Met Asn Thr Leu Arg Ala Leu Arg
30 35 40cgc gac cct cag tcc gcc gag aac ttc ccg tcc gac gtt gtc gag ctg 197Arg Asp Pro Gln Ser Ala Glu Asn Phe Pro Ser Asp Val Val Glu Leu45 50 55 60aac tct tac ctc acc ggc cgt tcc cgt ctc cca ggg gtt aac gca att 245Asn Ser Tyr Leu Thr Gly Arg Ser Arg Leu Pro Gly Val Asn Ala Ile
65 70 75cca ggc aag ggc acc aac gta ccg ctg tac atc ttg ggt tca tcc ctc 293Pro Gly Lys Gly Thr Asn Val Pro Leu Tyr Ile Leu Gly Ser Ser Leu
80 85 90ttt ggt gca caa ttg gca gca cag ttg ggt atg cct tat tcc ttc gca 341Phe Gly Ala Gln Leu Ala Ala Gln Leu Gly Met Pro Tyr Ser Phe Ala
95 100 105tcc cac ttc gca cca act cac ctt gag cac gcg gtg caa acc tac cgg 389Ser His Phe Ala Pro Thr His Leu Glu His Ala Val Gln Thr Tyr Arg
110 115 120gat aac tac cag cct tca gag cag cat cct gag cct tat gtc att gcg 437Asp Asn Tyr Gln Pro Ser Glu Gln His Pro Glu Pro Tyr Val Ile Ala125 130 135 140gcc gtc aat gtc acc gca tct gat tcc act gaa caa gcc cac gat gat 485Ala Val Asn Val Thr Ala Ser Asp Ser Thr Glu Gln Ala His Asp Asp
145 150 155ttc tac aag gta gcg cgt gca cgc gtg aag aac atg gca ttg cgt ggc 533Phe Tyr Lys Val Ala Arg Ala Arg Val Lys Asn Met Ala Leu Arg Gly
160 165 170cga caa gtt act gat gag caa ctt gat gaa ctc atg gat tca cca gct 581Arg Gln Val Thr Asp Glu Gln Leu Asp Glu Leu Met Asp Ser Pro Ala
175 180 185gct cgc caa att gtc gac atg ctt cac tac acc gct ata ggc act gga 629Ala Arg Gln Ile Val Asp Met Leu His Tyr Thr Ala Ile Gly Thr Gly
190 195 200tcc gaa gtt aaa gaa tac cta gac ggt ttt gta aag acg gca cag gct 677Ser Glu Val Lys Glu Tyr Leu Asp Gly Phe Val Lys Thr Ala Gln Ala205 210 215 220gat gaa ctg atg atc tcc ctg caa tcc ccc aac act gaa gca acc acg 725Asp Glu Leu Met Ile Ser Leu Gln Ser Pro Asn Thr Glu Ala Thr Thr
225 230 235cgc aat atg gaa att ctt gcg gat gcg tgg att aat tagtaccgat 771Arg Asn Met Glu Ile Leu Ala Asp Ala Trp Ile Asn
240 245gggccggtag aca 784<210>430<211>248<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>430Met Leu Pro Asn His Ser Pro Tyr Val Ile Ala Glu Gln Phe Gly Thr1 5 10 15Leu Ala Glu Leu Tyr Pro Asp Arg Ile Asp Leu Gly Met Gly Arg Ala
20 25 30Pro Gly Thr Asp Met Asn Thr Leu Arg Ala Leu Arg Arg Asp Pro Gln
35 40 45Ser Ala Glu Asn Phe Pro Ser Asp Val Val Glu Leu Asn Ser Tyr Leu
50 55 60Thr Gly Arg Ser Arg Leu Pro Gly Val Asn Ala Ile Pro Gly Lys Gly65 70 75 80Thr Asn Val Pro Leu Tyr Ile Leu Gly Ser Ser Leu Phe Gly Ala Gln
85 90 95Leu Ala Ala Gln Leu Gly Met Pro Tyr Ser Phe Ala Ser His Phe Ala
100 105 110Pro Thr His Leu Glu His Ala Val Gln Thr Tyr Arg Asp Asn Tyr Gln
115 120 125Pro Ser Glu Gln His Pro Glu Pro Tyr Val Ile Ala Ala Val Asn Val
130 135 140Thr Ala Ser Asp Ser Thr Glu Gln Ala His Asp Asp Phe Tyr Lys Val145 150 155 160Ala Arg Ala Arg Val Lys Asn Met Ala Leu Arg Gly Arg Gln Val Thr
165 170 175Asp Glu Gln Leu Asp Glu Leu Met Asp Ser Pro Ala Ala Arg Gln Ile
180 185 190Val Asp Met Leu His Tyr Thr Ala Ile Gly Thr Gly Ser Glu Val Lys
195 200 205Glu Tyr Leu Asp Gly Phe Val Lys Thr Ala Gln Ala Asp Glu Leu Met
210 215 220Ile Ser Leu Gln Ser Pro Asn Thr Glu Ala Thr Thr Arg Asn Met Glu225 230 235 240Ile Leu Ala Asp Ala Trp Ile Asn
245<210>431<211>825<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(802)<223>RXA00332<400>431aatgaactct ggaaccgcca tgcagcaaaa cctctccaat tggtaatctt tgactcccag 60gttacgccag ccctgcgaca ccaccatcta gggttagagt atg gcc ttc aac aaa 115
Met Ala Phe Asn Lys
1 5gcg tac gat gca ctt cgc gcc cct caa atc acc ctc gga ctc atg aca 163Ala Tyr Asp Ala Leu Arg Ala Pro Gln Ile Thr Leu Gly Leu Met Thr
10 15 20cca aac ggc cct gaa cta ggg cgc agt gaa atg gtt cca acc gaa aat 211Pro Asn Gly Pro Glu Leu Gly Arg Ser Glu Met Val Pro Thr Glu Asn
25 30 35agc atc gaa cta gcc ata caa gca gaa gct caa gga ttc aga ggc atg 259Ser Ile Glu Leu Ala Ile Gln Ala Glu Ala Gln Gly Phe Arg Gly Met
40 45 50tgg gtt cga gac gtt cca ctc gca gtt cct caa gga atc act gtt acc 307Trp Val Arg Asp Val Pro Leu Ala Val Pro Gln Gly Ile Thr Val Thr
55 60 65gat aaa cag gct acg tat tta gat gat cca ttc tta atg ctc ggt gcg 355Asp Lys Gln Ala Thr Tyr Leu Asp Asp Pro Phe Leu Met Leu Gly Ala70 75 80 85atg gcc tct gtg acc tct aca atc gcg ctg ggc act gca gcg acc gtg 403Met Ala Ser Val Thr Ser Thr Ile Ala Leu Gly Thr Ala Ala Thr Val
90 95 100ctt cca ctc aga cat ccg cta cat gtg gcg aaa tcc gcg ctc acc ctt 451Leu Pro Leu Arg His Pro Leu His Val Ala Lys Ser Ala Leu Thr Leu
105 110 115gat cga ctc agc cac gga cgt ttc gtt tta ggc atc ggc tct ggc gac 499Asp Arg Leu Ser His Gly Arg Phe Val Leu Gly Ile Gly Ser Gly Asp
120 125 130agg cct gaa gaa ttc gag att ttt ggc aaa agc tta gac aat cga cgc 547Arg Pro Glu Glu Phe Glu Ile Phe Gly Lys Ser Leu Asp Asn Arg Arg
135 140 145gct gat att cag tct ggg tgg gca att ttg cgt gca gct ttg tcg ccg 595Ala Asp Ile Gln Ser Gly Trp Ala Ile Leu Arg Ala Ala Leu Ser Pro150 155 160 165gat cct gcg atg cgg gcc gac ctt gaa ttt gcg cca acc acg cca cct 643Asp Pro Ala Met Arg Ala Asp Leu Glu Phe Ala Pro Thr Thr Pro Pro
170 175 180gaa gct cag atc ccc atg atc gct gta ggt tct gcc cga caa aca gtg 691Glu Ala Gln Ile Pro Met Ile Ala Val Gly Ser Ala Arg Gln Thr Val
185 190 195caa tgg atc gcc cga aac gcc gac gga tgg gca acc tac tac cgc ccc 739Gln Trp Ile Ala Arg Asn Ala Asp Gly Trp Ala Thr Tyr Tyr Arg Pro
200 205 210gct gaa gct caa gtc gga cgc ctc gat ctc tgg gac aaa gcc cgt ggt 787Ala Glu Ala Gln Val Gly Arg Leu Asp Leu Trp Asp Lys Ala Arg Gly
215 220 225ggc acc cgc cct tgt tgatttcctc catggggctc aac 825Gly Thr Arg Pro Cys230<210>432<211>234<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>432Met Ala Phe Asn Lys Ala Tyr Asp Ala Leu Arg Ala Pro Gln Ile Thr1 5 10 15Leu Gly Leu Met Thr Pro Asn Gly Pro Glu Leu Gly Arg Ser Glu Met
20 25 30Val Pro Thr Glu Asn Ser Ile Glu Leu Ala Ile Gln Ala Glu Ala Gln
35 40 45Gly Phe Arg Gly Met Trp Val Arg Asp Val Pro Leu Ala Val Pro Gln
50 55 60Gly Ile Thr Val Thr Asp Lys Gln Ala Thr Tyr Leu Asp Asp Pro Phe65 70 75 80Leu Met Leu Gly Ala Met Ala Ser Val Thr Ser Thr Ile Ala Leu Gly
85 90 95Thr Ala Ala Thr Val Leu Pro Leu Arg His Pro Leu His Val Ala Lys
100 105 110Ser Ala Leu Thr Leu Asp Arg Leu Ser His Gly Arg Phe Val Leu Gly
115 120 125Ile Gly Ser Gly Asp Arg Pro Glu Glu Phe Glu Ile Phe Gly Lys Ser
130 135 140Leu Asp Asn Arg Arg Ala Asp Ile Gln Ser Gly Trp Ala Ile Leu Arg145 150 155 160Ala Ala Leu Ser Pro Asp Pro Ala Met Arg Ala Asp Leu Glu Phe Ala
165 170 175Pro Thr Thr Pro Pro Glu Ala Gln Ile Pro Met Ile Ala Val Gly Ser
180 185 190Ala Arg Gln Thr Val Gln Trp Ile Ala Arg Asn Ala Asp Gly Trp Ala
195 200 205Thr Tyr Tyr Arg Pro Ala Glu Ala Gln Val Gly Arg Leu Asp Leu Trp
210 215 220Asp Lys Ala Arg Gly Gly Thr Arg Pro Cys225 230<210>433<211>842<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(1)..(819)<223>RXA01838<400>433cag cac ctc tcc ggc ggc cgt gtt gac ctt atg atg ggc cgt ggc aac 48Gln His Leu Ser Gly Gly Arg Val Asp Leu Met Met Gly Arg Gly Asn1 5 10 15acc gga ccc gtt tac cca tgg ttt ggc aaa gac atc cac caa ggc atc 96Thr Gly Pro Val Tyr Pro Trp Phe Gly Lys Asp Ile His Gln Gly Ile
20 25 30cca cta gcg att gaa aac tac cac ctc ctg cgc cgc ctc tgg cgc gaa 144Pro Leu Ala Ile Glu Asn Tyr His Leu Leu Arg Arg Leu Trp Arg Glu
35 40 45gac gta gtc aac tgg cag ggc aaa ttc cgc aca ccg ttg cag gga tac 192Asp Val Val Asn Trp Gln Gly Lys Phe Arg Thr Pro Leu Gln Gly Tyr
50 55 60acc tct acc cca gca cca tta gac ggc gtt gca cca ttc gtc tgg cac 240Thr Ser Thr Pro Ala Pro Leu Asp Gly Val Ala Pro Phe Val Trp His65 70 75 80ggc tcc atc cgc tcc acc gaa atc gca gag caa gca gcc ttc tat ggc 288Gly Ser Ile Arg Ser Thr Glu Ile Ala Glu Gln Ala Ala Phe Tyr Gly
85 90 95gac ggc ttc ttc cac aac aac atc ttc tgg aac aaa gag cac acc gcc 336Asp Gly Phe Phe His Asn Asn Ile Phe Trp Asn Lys Glu His Thr Ala
100 105 110caa atg gtc aac ctc tac cgc cag cgt ttc gaa cac tac gga cac ggc 384Gln Met Val Asn Leu Tyr Arg Gln Arg Phe Glu His Tyr Gly His Gly
115 120 125caa gca gac cag gcc atc gtg gga ctc ggt ggc caa gtc ttc atc ggc 432Gln Ala Asp Gln Ala Ile Val Gly Leu Gly Gly Gln Val Phe Ile Gly
130 135 140gat tct gaa gaa gaa gca aag aag acc ttc cgc ccc tac ttc gac aac 480Asp Ser Glu Glu Glu Ala Lys Lys Thr Phe Arg Pro Tyr Phe Asp Asn145 150 155 160gcc cct gtc tac gga cac gga cca tca ctt gaa gat ttc tcc cgc ctg 528Ala Pro Val Tyr Gly His Gly Pro Ser Leu Glu Asp Phe Ser Arg Leu
165 170 175acc cca cta acc gtc ggt acc gct gag caa gtt atc gaa cgc acc atg 576Thr Pro Leu Thr Val Gly Thr Ala Glu Gln Val Ile Glu Arg Thr Met
180 185 190gaa ttc gcc gac tgg gta ggc gat tac cag cgc cag ctc ttc ctc atc 624Glu Phe Ala Asp Trp Val Gly Asp Tyr Gln Arg Gln Leu Phe Leu Ile
195 200 205gac cac gcc ggc ctg cca cta gaa atg gtc ctt gat cag atc gaa cgc 672Asp His Ala Gly Leu Pro Leu Glu Met Val Leu Asp Gln Ile Glu Arg
210 215 220ctc ggc cac gat gtc gtc cca gag gta cgc cgc cgc atg gag gag cgt 720Leu Gly His Asp Val Val Pro Glu Val Arg Arg Arg Met Glu Glu Arg225 230 235 240cgc cca gac cac gtt ccc tcc aac cca cca acc cac cag agc ctg aag 768Arg Pro Asp His Val Pro Ser Asn Pro Pro Thr His Gln Ser Leu Lys
245 250 255gcc aac cga aac agc cct tac ttt cag atc aac cct ggt cag cca act 816Ala Asn Arg Asn Ser Pro Tyr Phe Gln Ile Asn Pro Gly Gln Pro Thr
260 265 270gag tagtttttct gaaactaagg aga 842Glu<210>434<211>273<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>434Gln His Leu Ser Gly Gly Arg Val Asp Leu Met Met Gly Arg Gly Asn1 5 10 15Thr Gly Pro Val Tyr Pro Trp Phe Gly Lys Asp Ile His Gln Gly Ile
20 25 30Pro Leu Ala Ile Glu Asn Tyr His Leu Leu Arg Arg Leu Trp Arg Glu
35 40 45Asp Val Val Asn Trp Gln Gly Lys Phe Arg Thr Pro Leu Gln Gly Tyr
50 55 60Thr Ser Thr Pro Ala Pro Leu Asp Gly Val Ala Pro Phe Val Trp His65 70 75 80Gly Ser Ile Arg Ser Thr Glu Ile Ala Glu Gln Ala Ala Phe Tyr Gly
85 90 95Asp Gly Phe Phe His Asn Asn Ile Phe Trp Asn Lys Glu His Thr Ala
100 105 110Gln Met Val Asn Leu Tyr Arg Gln Arg Phe Glu His Tyr Gly His Gly
115 120 125Gln Ala Asp Gln Ala Ile Val Gly Leu Gly Gly Gln Val Phe Ile Gly
130 135 140Asp Ser Glu Glu Glu Ala Lys Lys Thr Phe Arg Pro Tyr Phe Asp Asn145 150 155 160Ala Pro Val Tyr Gly His Gly Pro Ser Leu Glu Asp Phe Ser Arg Leu
165 170 175Thr Pro Leu Thr Val Gly Thr Ala Glu Gln Val Ile Glu Arg Thr Met
180 185 190Glu Phe Ala Asp Trp Val Gly Asp Tyr Gln Arg Gln Leu Phe Leu Ile
195 200 205Asp His Ala Gly Leu Pro Leu Glu Met Val Leu Asp Gln Ile Glu Arg
210 215 220Leu Gly His Asp Val Val Pro Glu Val Arg Arg Arg Met Glu Glu Arg225 230 235 240Arg Pro Asp His Val Pro Ser Asn Pro Pro Thr His Gln Ser Leu Lys
245 250 255Ala Asn Arg Asn Ser Pro Tyr Phe Gln Ile Asn Pro Gly Gln Pro Thr
260 265 270Glu<210>435<211>1167<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1144)<223>RXA02643<400>435tgagaacacc taaaaacctt gcgggaatac caccaacccc atattgttga tatatctaca 60aactctttta ataagtctga tcaacaacgt gaggaaagca atg aaa aac gtc tcc 115
Met Lys Asn Val Ser
1 5ttc ggc ctc gac acc ttc ggc gac aac gcc atc gac ctg cag ggc aac 163Phe Gly Leu Asp Thr Phe Gly Asp Asn Ala Ile Asp Leu Gln Gly Asn
10 15 20ccg gtc tcc cct gca caa aca ctt cga aac atc att gat gaa gcc aag 211Pro Val Ser Pro Ala Gln Thr Leu Arg Asn Ile Ile Asp Glu Ala Lys
25 30 35atg gca gac aaa gtc ggg gtg gat atc atc ggc atc gga gag cac cac 259Met Ala Asp Lys Val Gly Val Asp Ile Ile Gly Ile Gly Glu His His
40 45 50cgt gag gaa tac tca gtt tct gca cct gac atc gtc atg aca gct atc 307Arg Glu Glu Tyr Ser Val Ser Ala Pro Asp Ile Val Met Thr Ala Ile
55 60 65ctc gca tcc act gag cga ctc aaa gtc acc tct tcc gtg act gtg ctg 355Leu Ala Ser Thr Glu Arg Leu Lys Val Thr Ser Ser Val Thr Val Leu70 75 80 85tcc tct gat gat cct gtt cgc ctg ttt gag cgt tat tcc acc atg aat 403Ser Ser Asp Asp Pro Val Arg Leu Phe Glu Arg Tyr Ser Thr Met Asn
90 95 100gca ctg tcc aac ggt cgc gcc gaa atc acc ttg gga cgc ggt tcc ttc 451Ala Leu Ser Asn Gly Arg Ala Glu Ile Thr Leu Gly Arg Gly Ser Phe
105 110 115att gag tct ttc cca ttg ttt ggt ttt gat ctt cag gac tac gag cag 499Ile Glu Ser Phe Pro Leu Phe Gly Phe Asp Leu Gln Asp Tyr Glu Gln
120 125 130ctg ttt agt gaa cgc ctt gat ttg ttc gcg aag att ctt gag gcc gac 547Leu Phe Ser Glu Arg Leu Asp Leu Phe Ala Lys Ile Leu Glu Ala Asp
135 140 145agc cgt ggt cag ggc gtg acc tgg cat ggt gag acc cgc tcg gcg ttg 595Ser Arg Gly Gln Gly Val Thr Trp His Gly Glu Thr Arg Ser Ala Leu150 155 160 165gaa aac cag atg ctt tac cca cca act gag aat ggc att cac gct tgg 643Glu Asn Gln Met Leu Tyr Pro Pro Thr Glu Asn Gly Ile His Ala Trp
170 175 180gtt gca gtg ggt ggc agc cca gaa tca gtc gtg cgc gct gct aag tat 691Val Ala Val Gly Gly Ser Pro Glu Ser Val Val Arg Ala Ala Lys Tyr
185 190 195cgt ttc ccg ttg atg ctt gcc atc atc ggc ggt gct cct gag cgt ttc 739Arg Phe Pro Leu Met Leu Ala Ile Ile Gly Gly Ala Pro Glu Arg Phe
200 205 210cgc ccg tat gtg gat ctg tac aag cgt gcc aac gaa cag ttc ggg cag 787Arg Pro Tyr Val Asp Leu Tyr Lys Arg Ala Asn Glu Gln Phe Gly Gln
215 220 225cct caa aag ccc att ggt gtg cac tcc cct gga ctc att gcg gca act 835Pro Gln Lys Pro Ile Gly Val His Ser Pro Gly Leu Ile Ala Ala Thr230 235 240 245gat gag gaa gcc cgt gag cta gca ctt aat gat tgg ttg gaa ctc caa 883Asp Glu Glu Ala Arg Glu Leu Ala Leu Asn Asp Trp Leu Glu Leu Gln
250 255 260cgc aag atc ggt gct gaa cgc ggt tgg gct cct gcg gat gca atg cag 931Arg Lys Ile Gly Ala Glu Arg Gly Trp Ala Pro Ala Asp Ala Met Gln
265 270 275ttt gaa cgc gaa atc gat cac ggt tcc tta tac atc ggt tcc cct gag 979Phe Glu Arg Glu Ile Asp His Gly Ser Leu Tyr Ile Gly Ser Pro Glu
280 285 290acg gtc gca aag aag atc gcc aaa acc att tca gtg ctt gat ctt gat 1027Thr Val Ala Lys Lys Ile Ala Lys Thr Ile Ser Val Leu Asp Leu Asp
295 300 305cgc ttt acc ctc aaa tac gcc agt ggc cag acc cct cat gag tac ttg 1075Arg Phe Thr Leu Lys Tyr Ala Ser Gly Gln Thr Pro His Glu Tyr Leu310 315 320 325ctg aag tcc att gag ttg tat ggc act gag gtt att ccg ctg gtg aag 1123Leu Lys Ser Ile Glu Leu Tyr Gly Thr Glu Val Ile Pro Leu Val Lys
330 335 340gac atc ttg acc aag cag gct taagaaggtc ttaggacatt ccc 1167Asp Ile Leu Thr Lys Gln Ala
345<210>436<211>348<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>436Met Lys Asn Val Ser Phe Gly Leu Asp Thr Phe Gly Asp Asn Ala Ile1 5 10 15Asp Leu Gln Gly Asn Pro Val Ser Pro Ala Gln Thr Leu Arg Asn Ile
20 25 30Ile Asp Glu Ala Lys Met Ala Asp Lys Val Gly Val Asp Ile Ile Gly
35 40 45Ile Gly Glu His His Arg Glu Glu Tyr Ser Val Ser Ala Pro Asp Ile
50 55 60Val Met Thr Ala Ile Leu Ala Ser Thr Glu Arg Leu Lys Val Thr Ser65 70 75 80Ser Val Thr Val Leu Ser Ser Asp Asp Pro Val Arg Leu Phe Glu Arg
85 90 95Tyr Ser Thr Met Asn Ala Leu Ser Asn Gly Arg Ala Glu Ile Thr Leu
100 105 110Gly Arg Gly Ser Phe Ile Glu Ser Phe Pro Leu Phe Gly Phe Asp Leu
115 120 125Gln Asp Tyr Glu Gln Leu Phe Ser Glu Arg Leu Asp Leu Phe Ala Lys
130 135 140Ile Leu Glu Ala Asp Ser Arg Gly Gln Gly Val Thr Trp His Gly Glu145 150 155 160Thr Arg Ser Ala Leu Glu Asn Gln Met Leu Tyr Pro Pro Thr Glu Asn
165 170 175Gly Ile His Ala Trp Val Ala Val Gly Gly Ser Pro Glu Ser Val Val
180 185 190Arg Ala Ala Lys Tyr Arg Phe Pro Leu Met Leu Ala Ile Ile Gly Gly
195 200 205Ala Pro Glu Arg Phe Arg Pro Tyr Val Asp Leu Tyr Lys Arg Ala Asn
210 215 220Glu Gln Phe Gly Gln Pro Gln Lys Pro Ile Gly Val His Ser Pro Gly225 230 235 240Leu Ile Ala Ala Thr Asp Glu Glu Ala Arg Glu Leu Ala Leu Asn Asp
245 250 255Trp Leu Glu Leu Gln Arg Lys Ile Gly Ala Glu Arg Gly Trp Ala Pro
260 265 270Ala Asp Ala Met Gln Phe Glu Arg Glu Ile Asp His Gly Ser Leu Tyr
275 280 285Ile Gly Ser Pro Glu Thr Val Ala Lys Lys Ile Ala Lys Thr Ile Ser
290 295 300Val Leu Asp Leu Asp Arg Phe Thr Leu Lys Tyr Ala Ser Gly Gln Thr305 310 315 320Pro His Glu Tyr Leu Leu Lys Ser Ile Glu Leu Tyr Gly Thr Glu Val
325 330 335Ile Pro Leu Val Lys Asp Ile Leu Thr Lys Gln Ala
340 345<210>437<211>726<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(703)<223>RXA01933<400>437ctagaagcct taggcaagaa atttggttga gttttcgatc tctacgacgc gtcatctcaa 60ttccacctag gcttggatgc aggttagaaa ggagccttcg atg tct aag act cgt 115
Met Ser Lys Thr Arg
1 5act ttt ctg ttt gat ctt tat ggt gtt ctc atc aag gag cat ggt gcg 163Thr Phe Leu Phe Asp Leu Tyr Gly Val Leu Ile Lys Glu His Gly Ala
10 15 20gcg cag ttt gag cgg gtt gcg cgt gcg gtg ggg gag ccg tcc aag aac 211Ala Gln Phe Glu Arg Val Ala Arg Ala Val Gly Glu Pro Ser Lys Asn
25 30 35gac aag ctg cat gag gtt tat gag tcg ctt cgt ctg gat ctg gat gcc 259Asp Lys Leu His Glu Val Tyr Glu Ser Leu Arg Leu Asp Leu Asp Ala
40 45 50ggc cgc gtg agt gag gtg aat tat tgg aat cag atc aaa cta ttg gtg 307Gly Arg Val Ser Glu Val Asn Tyr Trp Asn Gln Ile Lys Leu Leu Val
55 60 65ggt ttg gag ttt ttg gat atc cag gag gtc atc gcg gct gac tac agg 355Gly Leu Glu Phe Leu Asp Ile Gln Glu Val Ile Ala Ala Asp Tyr Arg70 75 80 85ggc ctt tat gag cgt gat cag gac atg gtt gat tat gtg ttg tcg ttg 403Gly Leu Tyr Glu Arg Asp Gln Asp Met Val Asp Tyr Val Leu Ser Leu
90 95 100aag gcg aaa ggc cac cgc atc gga att ttg tcg aat att ccg gag ggg 451Lys Ala Lys Gly His Arg Ile Gly Ile Leu Ser Asn Ile Pro Glu Gly
105 110 115ttg gcc aag ctg ttg aag gag cac aat tcg gag tgg ctt gat cag ctt 499Leu Ala Lys Leu Leu Lys Glu His Asn Ser Glu Trp Leu Asp Gln Leu
120 125 130gat gcg gtg act ttg tcg tgc gat att ggc gcg gcg aag ccg gag ccg 547Asp Ala Val Thr Leu Ser Cys Asp Ile Gly Ala Ala Lys Pro Glu Pro
135 140 145aag tct ttc cat gtg gca ctt gag gcc ctt ggt gaa aaa gct gag gat 595Lys Ser Phe His Val Ala Leu Glu Ala Leu Gly Glu Lys Ala Glu Asp150 155 160 165gtg acc ttt att gat gat cgc gtg cgt aac att gag gca gcg cgc gaa 643Val Thr Phe Ile Asp Asp Arg Val Arg Asn Ile Glu Ala Ala Arg Glu
170 175 180gaa ggt ctc agc aca att cac ttc act ggc tta gat tcc tta aaa gaa 691Glu Gly Leu Ser Thr Ile His Phe Thr Gly Leu Asp Ser Leu Lys Glu
185 190 195agc att cag gaa tgacacctca accactgatt ttg 726Ser Ile Gln Glu
200<210>438<211>201<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>438Met Ser Lys Thr Arg Thr Phe Leu Phe Asp Leu Tyr Gly Val Leu Ile1 5 10 15Lys Glu His Gly Ala Ala Gln Phe Glu Arg Val Ala Arg Ala Val Gly
20 25 30Glu Pro Ser Lys Asn Asp Lys Leu His Glu Val Tyr Glu Ser Leu Arg
35 40 45Leu Asp Leu Asp Ala Gly Arg Val Ser Glu Val Asn Tyr Trp Asn Gln
50 55 60Ile Lys Leu Leu Val Gly Leu Glu Phe Leu Asp Ile Gln Glu Val Ile65 70 75 80Ala Ala Asp Tyr Arg Gly Leu Tyr Glu Arg Asp Gln Asp Met Val Asp
85 90 95Tyr Val Leu Ser Leu Lys Ala Lys Gly His Arg Ile Gly Ile Leu Ser
100 105 110Asn Ile Pro Glu Gly Leu Ala Lys Leu Leu Lys Glu His Asn Ser Glu
115 120 125Trp Leu Asp Gln Leu Asp Ala Val Thr Leu Ser Cys Asp Ile Gly Ala
130 135 140Ala Lys Pro Glu Pro Lys Ser Phe His Val Ala Leu Glu Ala Leu Gly145 150 155 160Glu Lys Ala Glu Asp Val Thr Phe Ile Asp Asp Arg Val Arg Asn Ile
165 170 175Glu Ala Ala Arg Glu Glu Gly Leu Ser Thr Ile His Phe Thr Gly Leu
180 185 190Asp Ser Leu Lys Glu Ser Ile Gln Glu
195 200<210>439<211>1039<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(1039)<223>RXA02351<400>439tgacacttta cagactggtt ttcaactaat gacaccgaaa gaaatacacc tcaacctttt 60tgctttcggt gccgggcacc acgcggcggc gtggcgagcg gtg gag gga agc gtc 115
Val Glu Gly Ser Val
1 5gaa aag ctg ggt tta att tcc tgg tgg gag gaa ctc gcg cgc acc gct 163Glu Lys Leu Gly Leu Ile Ser Trp Trp Glu Glu Leu Ala Arg Thr Ala
10 15 20gag cgg ggc aag ctg gat gcg gtc ttt ttg gcc gat ggg cag gcg att 211Glu Arg Gly Lys Leu Asp Ala Val Phe Leu Ala Asp Gly Gln Ala Ile
25 30 35aat ccg gtc ggt ctg gag aat ggg ccg ggc tgg ttt ttg gag ccg gtg 259Asn Pro Val Gly Leu Glu Asn Gly Pro Gly Trp Phe Leu Glu Pro Val
40 45 50acc gcg ttg act gcg atg gcg cgg gcg acg aac aat att ggg ttg atc 307Thr Ala Leu Thr Ala Met Ala Arg Ala Thr Asn Asn Ile Gly Leu Ile
55 60 65agc aca att tcc agt acg ttt tgg cag ccg ttt cat gcg gcg cgg atg 355Ser Thr Ile Ser Ser Thr Phe Trp Gln Pro Phe His Ala Ala Arg Met70 75 80 85atc gcc agc ttg gat cat att tcg ggt ggg cgt gct gga atc aat gtg 403Ile Ala Ser Leu Asp His Ile Ser Gly Gly Arg Ala Gly Ile Asn Val
90 95 100gtg aca tcg atg acc gat gcg gag gcg cgt aac cac ggg atg gat gcg 451Val Thr Ser Met Thr Asp Ala Glu Ala Arg Asn His Gly Met Asp Ala
105 110 115ttg ccg ggt cac gat gtt cgc tat gcg cgc gct gcg gaa ttt att gaa 499Leu Pro Gly His Asp Val Arg Tyr Ala Arg Ala Ala Glu Phe Ile Glu
120 125 130acc atc act gcg ctg tgg gat tct tgg cct gcg gaa agt ttg gtg atg 547Thr Ile Thr Ala Leu Trp Asp Ser Trp Pro Ala Glu Ser Leu Val Met
135 140 145gat cgt gct gga aaa ttt gcg gac tcc tcg ctc att aaa tct atc gat 595Asp Arg Ala Gly Lys Phe Ala Asp Ser Ser Leu Ile Lys Ser Ile Asp150 155 160 165cat gat ggt gag ttc ttc caa gtc gct ggt ccg ctg aat atc ccc agt 643His Asp Gly Glu Phe Phe Gln Val Ala Gly Pro Leu Asn Ile Pro Ser
170 175 180cct ccg cag ggt cga ccc gta ctt ttt cag gct gga tcc tca ccg caa 691Pro Pro Gln Gly Arg Pro Val Leu Phe Gln Ala Gly Ser Ser Pro Gln
185 190 195gga cgg gaa atc gct gcg aaa tac gcc gag gca att tac tct gtg gcg 739Gly Arg Glu Ile Ala Ala Lys Tyr Ala Glu Ala Ile Tyr Ser Val Ala
200 205 210tgg gat ttg gag caa gcg caa gat tat cgc tct gat att cat gct cgt 787Trp Asp Leu Glu Gln Ala Gln Asp Tyr Arg Ser Asp Ile His Ala Arg
215 220 225gcc act gcc cag ggt cgc gag ccc atg ccg gtg ctt cct ggt ttg gtg 835Ala Thr Ala Gln Gly Arg Glu Pro Met Pro Val Leu Pro Gly Leu Val230 235 240 245act ttt gtt ggc acg acc gtg gaa gaa gcg cgt gca aaa cag cag gct 883Thr Phe Val Gly Thr Thr Val Glu Glu Ala Arg Ala Lys Gln Gln Ala
250 255 260ctt aat gcg ttg ctg ccg gtc aaa gac tca cta aat cag ttg agt ttc 931Leu Asn Ala Leu Leu Pro Val Lys Asp Ser Leu Asn Gln Leu Ser Phe
265 270 275ttt gtg ggt caa gat tgc tcg acg tgg gat ttg gat gca cct ccc cca 979Phe Val Gly Gln Asp Cys Ser Thr Trp Asp Leu Asp Ala Pro Pro Pro
280 285 290cca ctg cca ccg cta gaa gag ttt tcc ggt cct aaa ggc agg tac gaa 1027Pro Leu Pro Pro Leu Glu Glu Phe Ser Gly Pro Lys Gly Arg Tyr Glu
295 300 305acg gtc ctg cgg 1039Thr Val Leu Arg310<210>440<211>313<212>PRT<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<400>440Val Glu Gly Ser Val Glu Lys Leu Gly Leu Ile Ser Trp Trp Glu Glu1 5 10 15Leu Ala Arg Thr Ala Glu Arg Gly Lys Leu Asp Ala Val Phe Leu Ala
20 25 30Asp Gly Gln Ala Ile Asn Pro Val Gly Leu Glu Asn Gly Pro Gly Trp
35 40 45Phe Leu Glu Pro Val Thr Ala Leu Thr Ala Met Ala Arg Ala Thr Asn
50 55 60Asn Ile Gly Leu Ile Ser Thr Ile Ser Ser Thr Phe Trp Gln Pro Phe65 70 75 80His Ala Ala Arg Met Ile Ala Ser Leu Asp His Ile Ser Gly Gly Arg
85 90 95Ala Gly Ile Asn Val Val Thr Ser Met Thr Asp Ala Glu Ala Arg Asn
100 105 110His Gly Met Asp Ala Leu Pro Gly His Asp Val Arg Tyr Ala Arg Ala
115 120 125Ala Glu Phe Ile Glu Thr Ile Thr Ala Leu Trp Asp Ser Trp Pro Ala
130 135 140Glu Ser Leu Val Met Asp Arg Ala Gly Lys Phe Ala Asp Ser Ser Leu145 150 155 160Ile Lys Ser Ile Asp His Asp Gly Glu Phe Phe Gln Val Ala Gly Pro
165 170 175Leu Asn Ile Pro Ser Pro Pro Gln Gly Arg Pro Val Leu Phe Gln Ala
180 185 190Gly Ser Ser Pro Gln Gly Arg Glu Ile Ala Ala Lys Tyr Ala Glu Ala
195 200 205Ile Tyr Ser Val Ala Trp Asp Leu Glu Gln Ala Gln Asp Tyr Arg Ser
210 215 220Asp Ile His Ala Arg Ala Thr Ala Gln Gly Arg Glu Pro Met Pro Val225 230 235 240Leu Pro Gly Leu Val Thr Phe Val Gly Thr Thr Val Glu Glu Ala Arg
245 250 255Ala Lys Gln Gln Ala Leu Asn Ala Leu Leu Pro Val Lys Asp Ser Leu
260 265 270Asn Gln Leu Ser Phe Phe Val Gly Gln Asp Cys Ser Thr Trp Asp Leu
275 280 285Asp Ala Pro Pro Pro Pro Leu Pro Pro Leu Glu Glu Phe Ser Gly Pro
290 295 300Lys Gly Arg Tyr Glu Thr Val Leu Arg305 310
Claims (38)
1.一种得自谷氨酸棒杆菌(Corynebacterium glutamicum)的分离的核酸分子或其部分,所述核酸分子编码HA蛋白,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
2.权利要求1的分离核酸分子,其中所述核酸分子编码参与产生精细化学品的HA蛋白。
3.一种分离的谷氨酸棒杆菌核酸分子或其部分,所述核酸分子选自序列表奇数SEQ ID NO序列,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
4.一种分离的核酸分子,所述核酸分子编码选自序列表偶数SEQID NO序列的多肽序列,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
5.一种分离的核酸分子,所述核酸分子编码一种多肽的天然存在的等位基因变异体,所述多肽选自序列表偶数SEQ ID NO的序列的氨基酸序列,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
6.一种分离的核酸分子或其部分,所述核酸分子包含与选自序列表奇数SEQ ID NO序列的一个核苷酸序列具有至少50%同源性的核苷酸序列,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
7.一种分离的核酸分子,所述核酸分子包含一种包含选自序列表奇数SEQ ID NO序列中一个核苷酸序列的核酸的至少15个核苷酸的片段,前提是所述核酸分子不由表1给出的任何F标志的基因组成。
8.一种分离的核酸分子,所述核酸分子在严格条件下与权利要求1-7中任一项的核酸分子杂交。
9.一种分离的核酸分子,所述核酸分子包含权利要求1-8中任一项的核酸分子或其部分和一种编码异源多肽的核苷酸序列。
10.一种载体,所述载体包含权利要求1-9中任一项的核酸分子。
11.权利要求10的载体,所述载体为表达载体。
12.一种宿主细胞,所述宿主细胞是用权利要求11的表达载体转染的宿主细胞。
13.权利要求12的宿主细胞,其中所述细胞是一种微生物。
14.权利要求13的宿主细胞,其中所述细胞属于棒杆菌属(Corynebacterium)或短杆菌属(Brevibacterium)。
15.权利要求12的宿主细胞,其中所述核酸分子的表达导致对由所述细胞产生精细化学品进行调节。
16.权利要求15的宿主细胞,其中所述精细化学品选自:有机酸、生成蛋白质的氨基酸和非生成蛋白质的氨基酸、嘌呤和嘧啶碱基、核苷、核苷酸、脂质、饱和和不饱和脂肪酸、二元醇、糖类、芳族化合物、维生素、辅因子、聚酮化合物和酶。
17.一种生产多肽的方法,该方法包括在合适培养基中培养权利要求12的宿主细胞,由此生产所述多肽。
18.一种得自谷氨酸棒杆菌的分离的HA多肽或其部分。
19.权利要求18的多肽,其中所述多肽涉及精细化学品生产的产量。
20.一种分离的多肽,所述多肽包含选自序列表偶数SEQ ID NO序列的一个氨基酸序列,前提是所述氨基酸序列不由表1给出的任何F标志的基因编码。
21.一种分离的多肽或其部分,所述多肽包含一种含有选自序列表偶数SEQ ID NO序列中一个氨基酸序列的多肽的天然存在的等位基因变异体,前提是所述氨基酸序列不由表1给出的任何F标志的基因编码。
22.权利要求18-21中任一项的分离多肽,所述多肽还包含异源氨基酸序列。
23.一种分离的多肽,所述多肽由一种核酸分子编码,所述核酸分子包含与选自序列表奇数SEQ ID NO序列的一种核酸具有至少50%同源性的核苷酸序列,前提是所述核酸分子不由表1给出的任何F标志的核酸分子组成。
24.一种分离的多肽,所述多肽包含与选自序列表偶数SEQ IDNO序列中的一个氨基酸序列至少有50%同源性的氨基酸序列,前提是所述氨基酸序列不由表1给出的任何F标志的基因编码。
25.一种生产精细化学品的方法,所述方法包括培养含有权利要求12的载体的细胞,由此生产所述精细化学品。
26.权利要求25的方法,其中所述方法还包括从所述培养物回收所述精细化学品的步骤。
27.权利要求25的方法,其中所述方法还包括用权利要求11的载体转染所述细胞,产生含有所述载体的细胞的步骤。
28.权利要求25的方法,其中所述细胞属于棒杆菌属或短杆菌属。
29.权利要求25的方法,其中所述细胞选自:谷氨酸棒杆菌(Corynebacterium glutamicum)、力士棒杆菌(Corynebacteriumherculis)、百合花棒杆菌(Corynebacterium lilium)、嗜乙酰乙酸棒杆菌(Corynebacterium acetoacidophilum)、醋谷棒杆菌(Corynebacteriumacetoglutamicum)、嗜乙酰棒杆菌(Corynebacterium acetophilutm)、产氨棒杆菌(Corynebacterium ammoniagenes)、Corynebacteriumfujiokense、Corynebacterium nitrilophilus、产氨短杆菌(Brevibacteriumammoniagenes)、Brevibacterium butanicum、谷氨酸棒杆菌(Brevibacterium divaricatum)、黄色短杆菌(Brevibacterium flavum)、希氏短杆菌(Brevibacterium healii)、酮戊二酸短杆菌(Brevibacteriumketoglutamicum)、Brevibacterium ketosoreductum、乳发酵短杆菌(Brevibacterium lactofermentum)、扩展短杆菌(Brevibacterium linens)、Brevibacterium paraffinolyticum和表3中给出的菌株。
30.权利要求25的方法,其中来自所述载体的所述核酸分子的表达导致对所述精细化学品的生产产生调节。
31.权利要求25的方法,其中所述精细化学品选自:有机酸、生成蛋白质的氨基酸和非生成蛋白质的氨基酸、嘌呤和嘧啶碱基、核苷、核苷酸、脂质、饱和和不饱和脂肪酸、二元醇、糖类、芳族化合物、维生素、辅因子、聚酮化合物和酶。
32.权利要求25的方法,其中所述精细化学品为氨基酸。
33.权利要求32的方法,其中所述氨基酸选自:赖氨酸、谷氨酸、谷氨酰胺、丙氨酸、天冬氨酸、甘氨酸、丝氨酸、苏氨酸、甲硫氨酸、半胱氨酸、缬氨酸、亮氨酸、异亮氨酸、精氨酸、脯氨酸、组氨酸、酪氨酸、苯丙氨酸和色氨酸。
34.一种生产精细化学品的方法,所述方法包括培养其基因组DNA因为含有权利要求1-9中任一项的核酸分子而改变的细胞。
35.一种诊断受治疗者体内白喉棒杆菌的存在或其活性的方法,所述方法包括检测所述受治疗者体内是否存在一种或多种序列表SEQ ID NO 1-440的序列,前提是所述序列不是表1给出的任何F标志的序列或由其编码,由此诊断所述受治疗者体内白喉棒杆菌的存在或活性。
36.一种宿主细胞,所述宿主细胞包含选自序列表奇数SEQ IDNO所述核酸分子的一种核酸分子,其中所述核酸分子被破坏。
37.一种宿主细胞,所述宿主细胞包含选自序列表奇数SEQ IDNO所述核酸分子的一种核酸分子,其中所述核酸分子在序列表的所述奇数SEQ ID NO序列中包含一个或多个核酸修饰。
38.一种宿主细胞,所述宿主细胞包含选自序列表奇数SEQ IDNO所述核酸分子的一种核酸分子,其中相对于所述核酸分子的野生型调节区而言,所述核酸分子的调节区被修饰。
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