CN1345533A - Production method for trilinear hybrid rice seed and application thereof - Google Patents
Production method for trilinear hybrid rice seed and application thereof Download PDFInfo
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- CN1345533A CN1345533A CN 00117447 CN00117447A CN1345533A CN 1345533 A CN1345533 A CN 1345533A CN 00117447 CN00117447 CN 00117447 CN 00117447 A CN00117447 A CN 00117447A CN 1345533 A CN1345533 A CN 1345533A
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Abstract
The invention relates to a producing method and relevant planting technique for seed of ternary hybrid rice. Its features are as follows. Sterile line breed with sound quality and/or restoring line breed with sound quality is adopted to produce hybridized seed. Since the 'extensive renew 3550' is adopted as one of restoring line parent stain or hybridized parent strain of restoring line parent strain, thus sensitization type of ternary hybrid rice is obtained. The invention provides advantages of prolonging growth period of time, increasing, output. The hybrid rice seed can be used as seeds of single-crop rice or double-crop rice applied to production.
Description
The present invention relates to a kind of seed production method and corresponding culture technique thereof of ternary hybrid rice.
At present the main path of domestic and international indica type rice heterosis utilization is based on the three line method (male sterile line, maintenance line, recovery system) of nucleo-cytoplasmic interreaction male sterility system and based on the two line method of conditionity caryon sterile line (Photosensitive, light temp.-sensitive type or low form caryon sterile line and recover system).Since China in 1973 at first realized the indica hybrid rice three series mating, present technique field China kept world lead level always.70 and the eighties, China's Shan major clique prestige major clique system ternary hybrid rice of unifying is rapidly developed; In the nineties, unify association's major clique system ternary hybrid rice in Anhui of the rich major clique in the II major clique system in the ridge major clique in Sichuan system, Hunan, major clique system, Guangxi has covered national most of long-grained nonglutinous rice district on producing.The low thermo-sensitive type conditionity caryon sterile line that Hunan in 1992 is bred is trained short 64S makes civitas terrena's two-line hybrid rice step into the production application stage.In recent years, China's indica hybrid rice year cultivated area has reached 1,500 ten thousand hectares, and wherein ternary hybrid rice accounts for 96%.Because there is the seed production purity risk in the conditionity caryon sterile line of two-line hybrid rice at present, and produce to go up problems such as the conditionity caryon sterile line that can support utilization is less, therefore expectation remains the main path of indica type rice heterosis utilization at quite a long period three line method from now on.
Yet what ternary hybrid rice production was emphasized traditionally is high yield, and this has caused the quality of hybrid rice to can not get for a long time paying attention to, so its poor quality is ten minutes distinct issues in the Rice Production always.
First purpose of the present invention is to improve the quality of ternary hybrid rice rice.
Second purpose of the present invention is to improve its output on the basis of improving the hybrid rice rice quality.
First purpose of the present invention be achieved in that a kind of trilinear hybrid rice seed production method its to adopt male sterile line, maintenance line, recovery be triple crossing, it is characterized in that: male sterile line adopts colory parent (1).In three of hybrid rice is, the main aspect that influences quality is its male sterile line parent (promptly maternal), it occupies 2/3 hereditary factor (promptly having accounted for dliploid) in hybrid rice seed triploid endosperm, therefore, be the main aspect that improves the hybrid rice quality to male sterile line parent's (1) quality-improving.
First purpose of the present invention can be perfect like this: recover system and adopt colory parent (2), parent (1) can be rich A in Guangdong or magnificent rich A, and parent (2) can be wide extensive 3550 or wide extensive 688.The rich A in Guangdong, magnificent rich A, wide extensive 3550 and wide extensive 688 are Inst. of Rice, Guangdong Academy of Agricultural Sciences and cultivate successful hybridization rice parental breed in recent years.Wide extensive 3550 contain complementary sensitization gene, and anti-fertilizer, resistant to lodging; Form the crossbreed with photobehavior after itself and the male sterile line inter breed crossing best in quality, wide extensive 688 kinds are that wide extensive 3550 * wide extensive 468 hybridization are bred, and it does not still contain the kind of complementary sensitization gene, and quality is effectively improved; Photosensitive type crossbreed best in quality is bred in itself and male sterile line parent best in quality hybridization.Best in quality and the tool photosensitivity trilinear hybrid rice seed that the present invention breeds both can be used as the plantation of single cropping rice seed, also can be used as the plantation of double cropping late rice seed.
Second purpose of the present invention was achieved in that above-mentioned hybrid rice seed before traditional sowing time of temperature sensitive type rice varieties, the sowing (10-15 days is preferred lead) in-15 days that can shift to an earlier date 10 days, if as the single cropping rice seed time spent, lead can also strengthen, for example: can carry out broadcasting of seed of the present invention immediately after wheat or season hybrid rice harvesting early during as winter wheat and season hybrid rice crop rotation early and plant.Owing to taked above-mentioned perfect, therefore, prolonged the vegetative period of hybrid rice effectively, be that the starch of paddy or other physical and chemical quality proterties etc. all are improved and improve, promptly quality and yield all is improved, especially under the situation of double cropping rice, it not only can not influence the growth of early rice, and shifted to an earlier date the sowing season of late season rice effectively, thus effectively improving under the prerequisite of original ternary hybrid rice quality, increased the output of back season paddy rice.
The present invention on the basis of traditional ternary hybrid rice breeding, selected colory male sterile line (female parent) parent (1) for use and recover (male parent) parent (2) of system [parent (1) especially, its account for hybrid seed fine quality gene 2/3].Thereby solved the not good enough difficult problem of traditional ternary hybrid rice quality.Again because parent of the present invention (2) has adopted wide extensive 3550 or its wide extensive 688 and the male sterile line parent's (1) that contain same sensitization gene who derives of containing complementary sensitization gene hybridization, make the hybrid rice of breeding become photosensitive type from temperature sensitive type, thereby prolonged the full vegetative period of tool fine quality hybrid rice effectively, reached the purpose of high-quality, volume increase.
Embodiment 1:
Parent (1): the rich A in Guangdong (Guangdong Academy of Agricultural Sciences's cultivation)
Parent (2): characteristic trait and the cultivating process thereof of wide extensive 688 (Guangdong Academy of Agricultural Sciences's cultivation) rich A in Guangdong * wide extensive 688 → Feng You 688 (three line hybrid seed) rich A in Guangdong:
These male sterile line thousand group of hill body sterile plant rates reach 100%, and the pollen sterility degree reaches 99.95%; Sow at the beginning of 3 months in Guangzhou to the fringe fate that begins be 87-90 days; Sow at the beginning of 1 month in Sanya, Hainan to the fringe fate that begins be 86-91 days; In Guangzhou late July sowing, broadcasting the beginning fate is 73-75 days; The about 80cm of normal plant height of the rich A in Guangdong; Leaf look pale green; Grain has a small amount of tip awn; Thousand kernel weight 22.5-23.5 gram, the brown rice aspect ratio is 3.4-3.7; Appearance transparent; Chalk rice rate and chalkiness degree are 0; About amylose content 15%; About glue denseness 90mm; Meal is sliding soft, thick flavor; The column cap lacteous, stigma exposing ratio is about 80%, and outcrossing seed-setting rate can reach more than 40%.The rich A in Guangdong to breed process as follows: made the maternal wild abortion type maintenance line of breeding with domestic Anhui with the disease-resistant wild abortion type maintenance line of International Rice Research Institute's high-quality IR58025B and assist blue or green B hybridization, the disease-resistant new maintenance line of directive breeding high-quality in 1992.Annual through season 2 continuous seed selections of generation sooner or later in Guangzhou, early make in nineteen ninety-five and to breed the economical character F that tends towards stability
5For strain is 18.Make evening nineteen ninety-five in these strain systems and select fine individual plant and Zhenshan 97a (the wild abortion type male sterile cytoplasm source donor) biparental cross that meets the male sterile line objective trait; Early made plantation F in 1996
1, select the thorough individual plant of pollen abortion to carry out continuous backcross through microscopy; Evening in 1997 made at B
4F
1Generation have 3 transformation colony sterility thoroughly and economical character tend towards stability, wherein area code is used to new combination and surveys and join for the 97-2925 backcross population is numbered 25A, is named as the rich A in Guangdong in 1998, corresponding maintenance line male parent is named as the rich B in Guangdong.Wide extensive 688 characteristic trait and cultivating process thereof:
This new restorer line is broadcast beginning fringe fate at the beginning of Guangzhou is early made 3 months be 100 ± 3 days, and broadcasting beginning fringe fate mid-December in winter in Sanya, Hainan is 100 ± 3 days, extensively extensive 3550 similar with one of its parent basically; A tool type of growing up, about thousand kernel weight 26.5 grams, brown rice aspect ratio 3.2, outward appearance is translucent, and few chalk is white; Sword-like leave is short thick very narrow, and the stem stalk is sturdy, and fertilizer resistance anti-lodging is strong; High blast resisting.Extensively extensive 688 is to be bred by wide extensive 468 * extensively extensive 3550 hybridization, and its seed selection process is: autumn in 1996 employing hot water emasculation method is extensively extensive 468 to make female parent and extensively extensive 3550 to do paternal hybrid breeding goal recovery system.Wherein parent wide extensive 468 has response to temperature, a type of growing up, rice exterior quality are excellent, leaf look pale green, grow prolifically, plant type is slightly loose and contain wild abortion type sterile series recovers gene; Wide extensive 3550 tool responses to temperature of parent but contain complementary sensitization gene and recover gene have high blast resisting, fertilizer resistance anti-lodging, the basic nutrition growth characteristics are strong and food flavor superior quality characteristic, but its brown rice grain type is short and thick, outward appearance chalk rice rate height.Winter in 1996 was planted above-mentioned F in Nan Fan base, Sanya, Hainan
1Crossbreed, the autumn in 1997 in Guangzhou to its F
2For group directional select to have the complementary excellent characteristic of parents the grain of growing up, the rice outward appearance is translucent, sword-like leave short, very, 16 of sturdy fine individual plants of stem stalk, annual season in evening in Guangzhou, winter, plantation added generation and directive breeding target individual plant in the Sanya.From F
4In generation, played centering menu strain (strain system) when adding for seed selection, and also the rice blast resistance in lesion centering menu strain carries out Screening and Identification.Autumn in 1999 was bred a comparatively stable elite plant strain 96-468-3550-8, was named wide extensive 688.
The characteristic trait and the cultivating process of Feng You 688 three line hybrid seeds:
Feng You 688 made male parent (96-468-3550-8) with wide extensive 688 in 1998 to make the first generation cross-fertilize seed that maternal (97-2925) hybridization is bred with the rich A in Guangdong.This crossbreed plant height 100-105mm; The stem stalk is sturdy, and the plant type compactness is lifted straight and uprightly on the sword-like leave, and fertilizer resistance anti-lodging is strong; Doing early to make at the beginning of 3 months sowing performance sensitization can not normally ear in Guangzhou; Can sowing before and after the July 10 in Guangzhou, generally go up the temperature sensitive type kind or the combination of temperature sensitive type hybrid rice was sowed in 10-15 days ahead of time than producing.Feng You 688 is generally eared in early October; Thereby can prolong 10-15 days than temperature sensitive type kind its time of infertility or the longer time, thereby realize high yield.In cultivation, Feng You 688 every mu of land for growing field crops sowing quantities are 1kg, every mu of rice seedling bed seeding quantity 10-12.5kg, and length of time rice seedlings grow 15-20 days, seeding row spacing 16.5cm * 19.8cm, mu is planted 1.5-1.8 ten thousand caves, and mu is inserted basic seedling 60,000, and mu effectively fringe can reach ten thousand of 16-19; About 180 of general every fringes; Ripening rate is more than 85%; Thousand kernel weight 24-25 gram; General medium fertility per mu yield reaches more than the 500kg, and the high-yield culturing per mu yield can reach more than the 600kg; Later stage cold resistance is strong; The brown rice length and width are than about 3.2; Outward appearance is translucent, and chalk rice rate is less; Amylose content is medium, and the glue denseness is soft, and the food mouth is good, meal flavor delicate fragrance; High blast resisting.Especially be fit to the south China double rice cropping system and do the late rice plantation.
The relatively more existing hybrid rice seed of this crossbreed has the following advantages:
(1) quality better not only has good cooking quality, and has the good surface appearance quality, and the main quality proterties reaches GB/T 17891-1999 high-quality paddy standard;
(2) fertilizer resistance anti-lodging output height has been coordinated high yield and high-quality contradiction preferably, improves about 5% than contrast combination output of the same type;
(3) rice blast resistance is strong, and the full group of rice blast dominant population resistance ratio is reached more than 90%;
(4) commodity class height, the hybrid rice combination more of the same type of commodity market price increases added value of product and reaches 0.4-0.50 unit/kg, and increasing the benefit reaches 30%-40%.
The production technology of ternary hybrid rice combination Feng You 688 crossbreed of present embodiment;
Paddy rice is a self pollination crop, is to produce first generation cross-fertilize seed but utilize male sterile line and recover.Male sterile line of the present invention, maintenance line and recovery system are this research institute and breed.
Below be the implementation process of example narration present embodiment with the area, Guangzhou:
(1) father and mother originally broadcast poor phase arrangement
Produce Feng You 688 crossbreed in Guangzhou season in evening, this proper seeding of father and mother is the essential condition of achieving success.It is parent-wide extensive 688 minutes 2 phases sowing that male parent is promptly recovered, and is generally 7-10 days at interval, and in July 10 and sowing on July 17, the length of time rice seedlings grow was generally 15-18 days as respectively.Female parent is that the rich A in male sterile line parent-Guangdong date of seeding is July 25, length of time rice seedlings grow 18-20 days.Maternal and 1 male parent time difference phase are-13 days, with 2 male parent time differences phase be-8 days.
(2) land for growing field crops production of hybrid seeds isolation condition and father and mother's one's own profession are than distance isolation requirement＞200-300 rice, and time difference insulation request is more than 20 days.
The capable ratio of this field planting of father and mother is 2: 12 or 2: 14, and the male parent seeding row spacing is 33.0cm * 16.5cm, and maternal seeding row spacing is 13.3cm * 16.5cm.Maternal basic seedling 4-6 ten thousand/mu.
(3) " 920 " operation technique
Rich A is more insensitive to " 920 " because of Guangdong, so " 920 " consumption should suitably strengthen general consumption 300g-375g/hm than Zhenshan 97a
2, divide secondary to use, first goes out fringe inferior to the rich A in Guangdong scotched the canopy leaf at about 10% to 15% o'clock earlier, was about 200-250g/hm by " 920 " consumption again
2Convert water and evenly spray father and mother originally, press 100-125g/hm after 2 days for the second time
2Use.
(4) pollination and results
2-3 day after " 920 " have sprayed, with the rope garland or rap male parent with the bamboo bar and carry out artificial supplementary pollination, this work generally needs 7-9 day approximately during every day male parent flowering.Treat that pollination finishes 25-30 day, during hybridization of female parent kind yellow maturity, choosing prescinds male parent, and the crossbreed of receiving again on the female parent supplies production application.Example 2: the characteristic trait of magnificent rich A * magnificent rich A of wide extensive rich 688 (three line hybrid seeds) of 688 → China:
These male sterile line thousand group of hill body sterile plant rates reach 100%, and the pollen sterility degree reaches 99.8%.Broadcasting beginning fringe fate at the beginning of 3 months in Guangzhou is 75-80 days, and broadcasting the fringe fate that begins in Guangzhou late July is 66-70 days; Its plant height 75-80cm; Leaf look pale green; About weight of thousand grain 23 grams; Brown rice aspect ratio 3.5; Outward appearance is translucent; The column cap milky, stigma exposing ratio reaches 87.5%.Outcrossing seed-setting rate can reach more than 50%.
It is identical with the rich A in Guangdong that it breeds process.Arrange to prolong 7 days in the poor phase of broadcasting that crossbreed produces than embodiment 1.
The other parts of present embodiment are identical with example 1.
Claims (7)
1. the production method of a trilinear hybrid rice seed, it adopts male sterile line, maintenance line, recovery is triple crossing, it is characterized in that: male sterile line adopts colory parent (1).
2. method according to claim 1 is characterized in that: parent (1) is the rich A in Guangdong.
3. method according to claim 1 is characterized in that: what recover system's employing is colory parent (2).
4. method according to claim 3 is characterized in that: parent (2) is wide extensive 3550.
5. method according to claim 4 is characterized in that: parent (2) is wide extensive 688.
6. method according to claim 2 is characterized in that: the triple crossing seed rice is to be bred with wide extensive 688 hybridization by the rich A of Austria.
7. the cultivation method of a claim 4,5 or 6 described hybrid rices: it is characterized in that:
Before the normal sowing time of temperature sensitive type rice varieties, sow in 10-15 days.
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| CNB001174479A CN1149915C (en) | 2000-09-26 | 2000-09-26 | Production method for trilinear hybrid rice seed and application thereof |
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| CN1149915C CN1149915C (en) | 2004-05-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218888B (en) * | 2007-12-04 | 2010-06-30 | 上海市农业生物基因中心 | Three-way cross combination propagation seed cultivating method for water-saving drought resisting hybrid rice |
| CN101218887B (en) * | 2007-12-04 | 2010-07-14 | 上海市农业生物基因中心 | Three-way cross combination breeding method for water-saving drought resisting hybrid rice |
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- 2000-09-26 CN CNB001174479A patent/CN1149915C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218888B (en) * | 2007-12-04 | 2010-06-30 | 上海市农业生物基因中心 | Three-way cross combination propagation seed cultivating method for water-saving drought resisting hybrid rice |
| CN101218887B (en) * | 2007-12-04 | 2010-07-14 | 上海市农业生物基因中心 | Three-way cross combination breeding method for water-saving drought resisting hybrid rice |
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