CN1307642A - 通过改变基因拷贝数制备多肽的方法 - Google Patents

通过改变基因拷贝数制备多肽的方法 Download PDF

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Publication number
CN1307642A
CN1307642A CN99808098A CN99808098A CN1307642A CN 1307642 A CN1307642 A CN 1307642A CN 99808098 A CN99808098 A CN 99808098A CN 99808098 A CN99808098 A CN 99808098A CN 1307642 A CN1307642 A CN 1307642A
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cell
polypeptide
nucleic acid
mutant
nucleotide sequence
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CN99808098A
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Chinese (zh)
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D·S·耶弗
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Novozymes Inc
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Novo Nordisk Biotech Inc
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Publication of CN1307642A publication Critical patent/CN1307642A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN99808098A 1998-05-27 1999-05-27 通过改变基因拷贝数制备多肽的方法 Pending CN1307642A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8550598A 1998-05-27 1998-05-27
US09/085,505 1998-05-27

Publications (1)

Publication Number Publication Date
CN1307642A true CN1307642A (zh) 2001-08-08

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Family Applications (1)

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CN99808098A Pending CN1307642A (zh) 1998-05-27 1999-05-27 通过改变基因拷贝数制备多肽的方法

Country Status (5)

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EP (1) EP1080210A2 (fr)
JP (1) JP2002516116A (fr)
CN (1) CN1307642A (fr)
AU (1) AU4213999A (fr)
WO (1) WO1999061651A2 (fr)

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JP2002535990A (ja) * 1999-02-02 2002-10-29 ノボザイムス バイオテック，インコーポレイティド 真菌細胞においてポリペプチドを生産する方法
CN1312260A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人蛋白水解酶调节蛋白10和编码这种多肽的多核苷酸
WO2002036830A2 (fr) * 2000-11-06 2002-05-10 Pepgen Corporation Procede permettant de determiner le nombre de copies d'un gene
US20030199038A1 (en) 2002-04-22 2003-10-23 Novozymes Biotech, Inc. Method for preparing polypeptide variants
ES2347658T3 (es) 2003-05-02 2010-11-03 Novozymes Inc. Variantes de beta-glucosidasas.
DE602004030807D1 (de) * 2003-08-22 2011-02-10 Suntory Holdings Ltd Verfahren zur züchtung eines lipidproduzierenden pilzes
US20050048619A1 (en) 2003-08-25 2005-03-03 Novozymes Biotech, Inc. Variants of glycoside hydrolases
DK1682656T3 (da) 2003-10-28 2013-11-18 Novozymes Inc Polypeptider med beta-glucosidaseaktivitet og polynukleotider, der koder for dem
CN1934249B (zh) 2004-01-16 2012-11-14 诺维信股份有限公司 降解木质素纤维素材料的方法
ES2469874T3 (es) 2004-01-30 2014-06-20 Novozymes Inc Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican
EP1766040B1 (fr) 2004-05-27 2017-08-09 Novozymes, Inc. Methodes permettant de transformer et de cribler l'expression de cellules de champignons filamenteux au moyen d'une bibliotheque d'adn
DK1877551T4 (da) 2005-04-27 2014-03-31 Novozymes Inc Polypeptider, der har endoglucanaseaktivitet, og polynukleotider, der koder for samme
NZ589570A (en) 2005-09-30 2012-06-29 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material
EP2004817A2 (fr) 2006-03-30 2008-12-24 Novozymes, Inc. Polypeptides ayant une activité endoglucanase et polynucléotides encodant lesdits polypeptides
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
WO2010059413A2 (fr) 2008-11-20 2010-05-27 Novozymes, Inc. Polypeptides ayant une activité amylolytique renforcée et polynucléotides codant pour ceux-ci
WO2010080527A1 (fr) 2008-12-19 2010-07-15 Novozymes, Inc. Procédés de détermination de l'amélioration de l'activité cellulolytique d'un polypeptide
US8604277B2 (en) 2009-01-28 2013-12-10 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
US8563268B2 (en) 2009-03-17 2013-10-22 Novozymes A/S Polypeptide having tyrosinase activity
CA2775967A1 (fr) 2009-10-23 2011-04-28 Novozymes, Inc. Variants d'une cellobiohydrolase et polynucleotides codant pour ceux-ci
MX2012005152A (es) 2009-11-06 2012-06-12 Novozymes Inc Composiciones para sacrificacion de material celulosico.
DK2496693T3 (en) 2009-11-06 2018-01-22 Novozymes Inc Polypeptides with cellobiohydrolase activity and polynucleotides encoding them
DK2496692T3 (en) 2009-11-06 2016-06-27 Novozymes Inc POLYPEPTIDES WITH xylanase AND POLYNUCLEOTIDES ENCODING THEM
DK2622068T3 (en) 2010-09-30 2016-10-17 Novozymes Inc Variants of polypeptides having cellulolytic enhancing ACTIVITY AND POLYNUCLEOTIDES ENCODING THEM
DK2622070T3 (en) 2010-09-30 2016-11-21 Novozymes Inc Variants of polypeptides having cellulolytic enhancing ACTIVITY AND POLYNUCLEOTIDES ENCODING THEM
EP2622069B1 (fr) 2010-10-01 2015-11-25 Novozymes, Inc. Variants de bêta-glucosidase et polynucléotides les codant
US9676830B2 (en) 2010-11-18 2017-06-13 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012103350A1 (fr) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides ayant une activité cellobiohydrolase et polynucléotides codant pour ceux-ci
EP2691519A1 (fr) 2011-03-31 2014-02-05 Novozymes, Inc. Variants du domaine de liaison à cellulose et polynucléotides codant pour ceux-ci
DK2702153T3 (en) 2011-04-28 2019-03-18 Novozymes Inc Polypeptides with endoglucanase activity and polynucleotides encoding them
US9624518B2 (en) 2011-04-29 2017-04-18 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
BR112014007651A2 (pt) 2011-09-30 2017-04-11 Novozymes Inc polipeptídeo quimérico isolado, polinucleotídeo isolado, métodos para produzir um polipeptídeo quimérico e um produto de fermentação, para degradar ou converter um material celulósico, e para fermentar um material celulósico, planta, parte de planta ou célula de planta transgênica, e, formulação de caldo inteiro ou composição de cultura de células
CN103998605B (zh) 2011-12-20 2021-08-03 诺维信股份有限公司 纤维二糖水解酶变体和编码它们的多核苷酸
EP4363598A1 (fr) * 2021-06-29 2024-05-08 Novozymes A/S Procédés de production de produits de fermentation utilisant des enzymes dégradant les fibres dans la fermentation

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FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
AU8171087A (en) * 1986-10-27 1988-05-25 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
US4956288A (en) * 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
AU645294B2 (en) * 1989-12-22 1994-01-13 Merck Serono Sa Endogenous gene expression modification with regulatory element
WO1992001800A1 (fr) * 1990-07-20 1992-02-06 Chiron Corporation Procede de transformation integrante de levure au moyen d'elements repetitifs disperses
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
JP4436934B2 (ja) * 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞

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Publication number Publication date
JP2002516116A (ja) 2002-06-04
WO1999061651A3 (fr) 2000-03-02
WO1999061651A2 (fr) 1999-12-02
EP1080210A2 (fr) 2001-03-07
AU4213999A (en) 1999-12-13

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