CN1286390C - Biological feed additive and its preparing process - Google Patents
Biological feed additive and its preparing process Download PDFInfo
- Publication number
- CN1286390C CN1286390C CNB001336959A CN00133695A CN1286390C CN 1286390 C CN1286390 C CN 1286390C CN B001336959 A CNB001336959 A CN B001336959A CN 00133695 A CN00133695 A CN 00133695A CN 1286390 C CN1286390 C CN 1286390C
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- CN
- China
- Prior art keywords
- bacterium liquid
- lactobacillus
- culture medium
- feed additive
- rice bran
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a technical field of a micro-organism feed additive, which is characterized in that enlarged culture for the first time and enlarged culture for the second time are carried out to sieved-extraction chlorinated lactobacillus, pure bacteria of lactobacillus coprophilus, yeast bacteria and bacillus subtilis by a culture medium; bacterium liquid is adsorbed by rice bran and wheat bran; heap fermentation and drying are carried out, and the biologic feed additive of which the number of bacterium types is 8 to 10 hundred millions/gram is obtained. The present invention has the advantages that the functions of a digestive system of an intestinal tract of livestock animals are improved to make the bacterium groups in the intestinal tract balanced; the dosage of medicines of the antibiotic class is reduced, the quality of livestock products is improved, and the absorption and the conversion rate of the livestock to the feed are improved; the feed is saved, the feed cost is reduced, the unfolding period is in advance, and the laying rate is improved. The present invention is a natural environment-protection and green environment-protection product.
Description
Technical field
The present invention relates to the additive for microbe feedstuff technical field, particularly biology feed additive of lactobacillus viridescens (Lactobacillusviridescens), lactobacillus coprophilus (Lactobacillus coprophilus), saccharomycete (Saccharomycescerevisiae), bacillus subtilis (Bacillus Subtilis) and preparation method thereof.
Background technology
At present, feed product is in the existing very great development of China, and some animal and fowl fodders have been realized commercialization, standardization.Additive as feed, all adopt lysine, methionine at home and abroad, and multivitamin and mineral matter, medicated premix also has some Chinese herb feed additives, and these additives are low to the livestock and poultry absorption rate, the side effect in addition that has, develop immunity to drugs, the biological chain of residual quantity directly enters edible human body, harm livestock and poultry and human life's health.
The microorganism of animal and bird intestines and feed in vivo digested and assimilated confidential relation; directly influence the synthetic of internal microorganism; keep the interior profitable strain of intestines just often; can prevent livestock and poultry to infect and the harmful bacterium of antagonism; keep microorganism species normalization in the enteron aisle; produce lactic acid, hydrogen peroxide and antibacterial material promote digesting and assimilating of nutriment.
Chinese invention patent 93110883, notification number 1089442 on July 20 1994 day for announcing, discloses a kind of " microorganism fodder additive Manufacturing approach and use; Disclose to sharp wax shape gemma and planted bacterium, lichengermium, Bacillus circulans, bacillus subtilis, bacillus firmus, bacillus megaterium is made the method for microorganism fodder additive.This patent has reasonably utilized bacillus as additive agent for feeding, but is bacillus, does not relate to the flora of other useful additive agent for feeding.
Summary of the invention
The objective of the invention is to be the profitable strain of reasonable use microorganism fodder additive more, cultivate the microorganism fodder additive of forming by three kinds of floras, and a kind of biology feed additive and preparation method thereof is provided.
The bacterial classification that the present invention adopts is known, can both obtain by the normal market channel, concrete goods catalogue is seen " bacterial classification catalogue " third edition, 1997, Chinese agriculture science and technology publishing house, is write by China Committee for Culture Collection of Microorganisms, typical case's culture collection committee of the Chinese Academy of Sciences, Institute of Microorganism, Academia Sinica, Chinese common micro-organisms culture presevation administrative center, in the book every kind of bacterial classification is all indicated source, characteristic, purposes, document, culture medium, cultivation temperature etc.
A kind of biology feed additive, it is characterized in that: lactobacillus viridescens (Lactobacillus viridescens), lactobacillus coprophilus (Lactobacillus coprophilus), saccharomycete (Saccharomyces cerevisiae) and bacillus subtilis (Bacillus subtilis) are purified through screening, the once expansion cultivation of culture medium, secondary enlarge cultivates, uses rice bran, wheat bran absorption bacterium liquid, heap fermentation, oven dry and reach the biology feed additive of bacterial classification quantity 8-10 hundred million/gram.
The preparation method of described biology feed additive is characterized in that:
1) purification lactobacillus viridescens, lactobacillus coprophilus, saccharomycete, bacillus subtilis saccharomycete and Bacillus subtilis strain are standby;
2) preparation is cultivated and is enlarged the culture of strains base, and its culture medium is made up of following material: magnesium sulfate 6g, sodium chloride 6g, potassium dihydrogen phosphate 15g, calcium carbonate 15g, V
C0.3g, VB
120.3g, VB
10.19g, peptone 5g, yeast extract 15g; Prepare after the culture medium, standby;
3) once enlarge cultivation: in culture medium, add lactobacillus viridescens (Lactobacilluscoprophilus), saccharomycete (Saccharomyces cerevisiae) and bacillus subtilis (Bacillussubtilis) bacterial classification of purifying and add in the culture tank, oxygenation, stirring, constant temperature 28-32 ℃, incubation time 16-24 hour, PH kept 6.5-7.5;
4) secondary enlarges cultivation: the amount expansion of culture medium is twice, add in the culture tank, it is neutral regulating pH value, oxygenation stirs, and constant temperature 28-32 ℃, incubation time 16-24 hour, pH value 6.5-7.5, become secondary and enlarge to cultivate bacterium liquid, secondary enlarges to be cultivated bacterium liquid and filters by 100 eye mesh screens, removes impurity, bacterium liquid is stand-by;
5) input rice bran of the bacterium liquid after the filtration or wheat bran adsorb, and the ratio of bacterium liquid and rice bran or wheat bran is 35%: 65% by weight percentage;
6) rice bran or wheat bran heap fermentation behind the absorption bacterium liquid are kept 25-35 ℃ of temperature, fermentation time 24 hours;
7) be the rice bran after the fermentation or wheat bran oven dry, bake out temperature＜80 ℃, moisture content is no more than 12%, and bacterial classification quantity 8-10 hundred million/gram is weighed to pack promptly gets product.
Advantage of the present invention is, improves the function of livestock and poultry animal enteron aisle digestive system, makes flora equilibrium in the enteron aisle, reduces the antibiotics use amount, improves the quality of animal products, improves absorption and the conversion ratio of livestock and poultry to feed; Save feed, reduce feed cost, marketing period shifts to an earlier date, and laying rate improves, and is natural environment-friendly and green environment-friendly products.
The specific embodiment
A kind of biology feed additive, it is enlarged cultivation, is adsorbed bacterium liquid with rice bran, wheat bran by screening purification lactobacillus viridescens, lactobacillus coprophilus, saccharomycete and bacillus subtilis once expansion cultivation, the secondary through culture medium, heap fermentation, oven dry and reach the biology feed additive of bacterial classification quantity 900,000,000/gram.
The preparation method of above-mentioned biology feed additive, made by following step:
1) screening purification lactobacillus viridescens, lactobacillus coprophilus, saccharomycete and Bacillus subtilis strain are standby;
2) preparation is cultivated and is enlarged culture of strains, and its culture medium is made up of following material: magnesium sulfate 6g, sodium chloride 6g, potassium dihydrogen phosphate 15g, calcium carbonate 15g, V
C0.3g, VB
120.3g, VB
10.19g, peptone 5g, yeast extract 15g.Prepare after the culture medium, standby;
3) once enlarge to cultivate: in culture medium, add lactobacillus viridescens, lactobacillus coprophilus, saccharomycete, the bacillus bacterial classification of purifying and add in the culture tank, 30 ℃ of oxygenations, stirring, constant temperature, incubation time 24 hours, PH keeps 7;
4) secondary enlarges cultivation: the amount expansion of culture medium is twice, adds in the culture tank, it is neutral regulating pH value, the oxygenation stirring, 30 ℃ of constant temperature, incubation time 12 hours, pH value 7, become secondary and enlarge cultivation bacterium liquid, secondary enlarges cultivation bacterium liquid and filters by 100 eye mesh screens, and it is stand-by to remove impurity bacterium liquid;
5) input rice bran of the bacterium liquid after the filtration or wheat bran adsorb, and the ratio of bacterium liquid and rice bran or wheat bran is 35%: 65% by weight percentage;
6) rice bran or wheat bran heap fermentation behind the absorption bacterium liquid are kept 30 ℃ of temperature, fermentation time 24 hours;
7) be the rice bran after the fermentation or wheat bran oven dry, bake out temperature＜80 ℃, moisture content is no more than 12%, and bacterial classification quantity 900,000,000/gram is weighed to pack promptly gets product.
Claims (2)
1. biology feed additive, it is characterized in that: lactobacillus viridescens, lactobacillus coprophilus, saccharomycete and bacillus subtilis are purified through screening, the once expansion cultivation of culture medium, secondary enlarge cultivates, uses rice bran, wheat bran absorption bacterium liquid, heap fermentation, oven dry and reach the biology feed additive of bacterial classification quantity 8-10 hundred million/gram.
2. the preparation method of biology feed additive according to claim 1 is characterized in that:
1) purification lactobacillus viridescens, lactobacillus coprophilus, saccharomycete, bacillus subtilis saccharomycete and Bacillus subtilis strain are standby;
2) preparation is cultivated and is enlarged the culture of strains base, and its culture medium is made up of following material: magnesium sulfate 6g, sodium chloride 6g, potassium dihydrogen phosphate 15g, calcium carbonate 15g, V
C0.3g, VB
120.3g, VB
10.19g, peptone 5g, yeast extract 15g; Prepare after the culture medium, standby;
3) once enlarge to cultivate: in culture medium, add lactobacillus viridescens, lactobacillus coprophilus, saccharomycete and the bacillus bacterial classification of purifying and add in the culture tank, oxygenation, stirring, constant temperature 28-32 ℃, incubation time 8-17 hour, PH kept 6.5-7.5;
4) secondary enlarges cultivation: the amount expansion of culture medium is twice, add in the culture tank, it is neutral regulating pH value, oxygenation stirs, and constant temperature 28-32 ℃, incubation time 8-12 hour, pH value 6.5-7.5, become secondary and enlarge cultivation bacterium liquid, secondary enlarges cultivation bacterium liquid and filters by 100 eye mesh screens, and it is stand-by to remove impurity bacterium liquid;
5) input rice bran of the bacterium liquid after the filtration or wheat bran adsorb, and the ratio of bacterium liquid and rice bran or wheat bran is 35%: 65% by weight percentage;
6) rice bran or wheat bran heap fermentation behind the absorption bacterium liquid are kept 25-35 ℃ of temperature, fermentation time 24 hours;
7) be the rice bran after the fermentation or wheat bran oven dry, bake out temperature＜80 ℃, moisture content is no more than 12%, and bacterial classification quantity 8-10 hundred million/gram is weighed to pack promptly gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001336959A CN1286390C (en) | 2000-12-04 | 2000-12-04 | Biological feed additive and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001336959A CN1286390C (en) | 2000-12-04 | 2000-12-04 | Biological feed additive and its preparing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1296765A CN1296765A (en) | 2001-05-30 |
| CN1286390C true CN1286390C (en) | 2006-11-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB001336959A Expired - Fee Related CN1286390C (en) | 2000-12-04 | 2000-12-04 | Biological feed additive and its preparing process |
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| CN (1) | CN1286390C (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297654C (en) * | 2003-03-14 | 2007-01-31 | 新疆威仕达生物工程股份有限公司 | Microbial ensiling strain and composite fungus, method for producing silage |
| CN100346709C (en) * | 2004-08-13 | 2007-11-07 | 内蒙古牧泉元兴饲料有限责任公司 | Process for preparing functional feed addictive |
| CN1989831B (en) * | 2005-12-28 | 2011-06-08 | 上海创博生态工程有限公司 | Microorganism feed addictive improving reproduction ability of boar and making method thereof |
| CN101103763B (en) * | 2006-07-14 | 2011-12-14 | 青岛康大镒泰科技有限公司 | Fodder-grade micro-ecological additive |
| CN101138392B (en) * | 2007-07-30 | 2011-08-17 | 湖南农业大学 | Micro-zoology preparations for feeding |
| CN101889635B (en) * | 2010-07-07 | 2012-11-21 | 格特生物制药（天津）有限公司 | Premix of yeast, bacillus subtilis and lactobacillus for livestock and poultry and preparation method thereof |
| CN102334602B (en) * | 2011-06-29 | 2013-04-17 | 重庆富博生物技术有限公司 | Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry |
| CN102356815A (en) * | 2011-09-24 | 2012-02-22 | 江西省赣粮实业有限公司 | Solid-state fermentation method for producing microecological feed with rice bran as main raw material |
| CN105211631A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Biology feed additive and preparation method thereof |
| CN104605139A (en) * | 2015-01-08 | 2015-05-13 | 河北农业大学 | Bacillus subtilis-acanthopanax senticosus polysaccharide synbiotics, preparation method thereof and application of bacillus subtilis-acanthopanax senticosus polysaccharide synbiotics as feed additive |
| CN104938769B (en) * | 2015-07-27 | 2019-04-05 | 江西省科学院微生物研究所 | A method of preparing rice bran strain preparation and its for feed fermentation |
| CN108522796A (en) * | 2018-03-29 | 2018-09-14 | 湖北吾尔利生物工程股份有限公司 | A kind of new bio feed prilling |
| CN111887346A (en) * | 2020-08-31 | 2020-11-06 | 苏州积光金豚环境科技有限公司 | Preparation method of biological ionized calcium feed additive |
| CN114097927A (en) * | 2021-11-23 | 2022-03-01 | 宜兴市天石饲料有限公司 | Preparation method of biological feed additive based on bacteriophage |
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