CN1284510A - 利用猪、牛、羊肺沉淀法生产肝素钠 - Google Patents
利用猪、牛、羊肺沉淀法生产肝素钠 Download PDFInfo
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- 239000003513 alkali Substances 0.000 abstract 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
本发明公开了一种利用猪、牛、羊肺替代树脂法,采用十六烷基三甲基溴化吡啶铵沉淀法提取肝素钠工艺。它由苯、铵、碱调解、分解、析出肝素-蛋白络合物沉淀加十六烷基溴化铵后离心分离,使肝素沉淀析出。从而使生产肝素钠的操作工艺难度降低,且提取率提高。
Description
本发明涉及一种采用长链季铵盐沉淀法提取肝素钠的新工艺。
目前,现有肝素钠生产工艺中,多数采用树脂法提取,原料为猪、羊小肠。而采用猪、牛、羊肺为原料生产肝素其中如:河南富程生化研究所采用D-254树脂吸附法。每1000克肺、只能提取肝素4.5万单位,效价70单位/毫克。
上海生化制药厂引进英国专利采用十六烷基吡定铵沉淀肝素,只能用于猪、羊小肠粘膜提取肝素。
台湾畜产研究所宜兰分所与台湾大学合作开发成功的利用猪肺生产工艺,属萃取法,其产量每1000克原料可提取肝素5万单位,效价恒定80单位/毫克,但其工艺操作比较复杂,提取率也未有大幅度提高。
本发明目的是提供一种方法简单,提取率高的生产肝素的工艺操作规程。
本发明是基于下面的原理而实现的:
肝素钠是一种硫酸氨基葡聚糖的钠盐,属粘多糖类物质。肝素在溶液中作为聚阴离子(有独特的临界盐浓度和高电荷密度)或者同各种阳离子(包括胺)结合而从水溶液中沉淀出来。
下面对本发明提出的利用猪、牛、羊肺沉淀肝素钠具体方式做进一步描述:
1、将猪、牛、羊肺体搅碎、磨浆加入等量的水、然后加热至一定温度、继续加入一定比例的甲苯、冷却、定时搅拌、加入硫酸铵水,制成溶液。加氢氧化钠调整PH值,水溶液加热至一定温度,保温一定时间,冷却过滤。
2、滤渣加入硫酸铵及水、混合液中加入氢氧化钠调整PH加热,过滤、冷却、加适度硫酸调整PH值,析出肝素一蛋白络合物沉淀。虹吸清液离心分离。固体用酒精洗涤、除脂肪、调整PH值,加氯化钠溶液,调整PH值,沉淀蛋白质,得黄色透明肝素溶液。
3、将黄色溶液调整PH值后加入蒸馏水、再加入十六烷基三甲基溴化铵溶液,搅拌静置,虹吸清液,沉淀物离心分离。继续加入十六烷基三甲基溴化铵、氯化钠溶液,乙醇、使肝素沉淀析出。脱色、除氧化物。稀释、再脱色、离心后加乙醇,静置后离心分离,丙酮洗涤后干燥、即为肝素钠。
大量实验表明,本发明提取肝素钠每1000克肺可提取肝素钠80000单位,效价恒定在90-95单位/毫克以上。
本发明写明了利用猪、牛、羊肺沉淀法生产肝素钠的工艺操作规程,用此方法生产肝素钠,提取率高且工艺简单,易于实际操作。
Claims (2)
1、一种替代树脂法、利用猪、牛、羊肺采用十六烷基溴化铵沉淀法提取肝素钠的工艺。
2、根据权利要求的工艺,其特征在于长链季铵盐沉淀析出肝素,使肝素外观白,提取率高、效价高。
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CN 99111447 CN1284510A (zh) | 1999-08-17 | 1999-08-17 | 利用猪、牛、羊肺沉淀法生产肝素钠 |
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CN 99111447 CN1284510A (zh) | 1999-08-17 | 1999-08-17 | 利用猪、牛、羊肺沉淀法生产肝素钠 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101218259B (zh) * | 2005-05-09 | 2011-06-15 | 赫普马林公司 | 制备低分子量肝素的方法 |
CN103951768A (zh) * | 2014-04-29 | 2014-07-30 | 贵州慧静生物科技有限公司 | 利用猪、牛肺提取肝素钠并联产多肽蛋白粉、氨基酸蛋白粉的方法 |
-
1999
- 1999-08-17 CN CN 99111447 patent/CN1284510A/zh active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101218259B (zh) * | 2005-05-09 | 2011-06-15 | 赫普马林公司 | 制备低分子量肝素的方法 |
CN103951768A (zh) * | 2014-04-29 | 2014-07-30 | 贵州慧静生物科技有限公司 | 利用猪、牛肺提取肝素钠并联产多肽蛋白粉、氨基酸蛋白粉的方法 |
CN103951768B (zh) * | 2014-04-29 | 2016-05-11 | 贵州慧静生物科技有限公司 | 利用猪、牛肺提取肝素钠并联产多肽蛋白粉、氨基酸蛋白粉的方法 |
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