CN1283171C - Selenium-rich probiotics foder additive production method and its product - Google Patents
Selenium-rich probiotics foder additive production method and its product Download PDFInfo
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Abstract
The present invention relates to a production method for a feed additive containing selenium enriched probiotics and a product, which belongs to the production field of feed additives. The feed additive takes candida utilis and acidophilic lactobacillus as bacterial strains. The candida utilis and the acidophilic lactobacillus are mixed according to the proportion of 1: 4 and are then inoculated in a culture medium containing sodium selenite. After organic selenium conversion accelerant is added and cultivated for 36h, the CFU numbers of the candida utilis and the acidophilic lactobacillus in the obtained product are respectively 10<9>/g and 10<11>/g, and the content of selenium is 50 mg/kg. The content of organic selenium is more than 90%, and the content of selenium methionine is more than 75%. 2kg (for sheep) of additives or 6kg (for pigs) of additives are added in feed of per ton when used as an additive. The feed additive exerts the double functions of the organic selenium and the probiotics, observably improves the animal production performance, enhances the animal immune function and the disease resistance, and improves the content (0.32 to 0.43 mg/kg) of selenium in animal muscles. The utility model provides a safe green feed additive which has no residue and pollution and is capable of replacing antibiotics.
Description
One, technical field
The present invention relates to production method of a kind of feed addictive that contains selenium-enriched probiotics and products thereof, belong to the feed addictive production field, be exclusively used in the production of feed with selenium-enriched probiotics, its product can be used as feed addictive, can bring into play the double action of organic selenium and probio.
Two, technical background
Selenium is one of animal and human's trace elements necessary.The growing of it and animal, breeding and disease have confidential relation.Studies show that that selenium has is anti-oxidant, improve immunity of organisms and premunition, regulates organism metabolism, influences animal and human's the function of breeding, antitumor, prevents and treats endemic disease (as Keshan disease, Kaschin-Beck disease), delays senility multiple functions such as the poisonous element of antagonism.
Most selenium is brought into play biological action with the form of selenocystein in the mammalian body in corresponding function albumen.Selenium is as many activated centres with selenium enzyme of important biomolecule function, and is closely related with functions such as the antioxidation of body and immune responses.At least found and be separated to 35 kinds of selenoproteins mammal at present, wherein the selenoprotein that function ratio is clearer and more definite is glutathione peroxidase (GPX), takes off iodine enzyme (ID), thioredoxin reductase (TR), selenium phosphorylation thing synzyme (SPS2), mitochondria of sperms film selenoprotein (MCS), sperm DNA in conjunction with selenoprotein, prostatic epithelium selenoprotein, human lymphocyte selenoprotein, selenoprotein P, selenoprotein W and a kind of 18ku selenoprotein that is present in the multiple tissue.They have the membrane structure of preventing and large biological molecule oxidative damage; Synthetic and regulate active thyroxine T3 level; Participate in the synthetic and adjusting DNA expression of DNA; Promote keeping of sperm generation, growth, maturation and vigor thereof; Protection endothelial cell and the normal function etc. of safeguarding musculature be physiological action widely.
Comprising a series of important biological function that promotes growth of animal, improves animal immune function and premunition and oxidation resistance just because of selenium has, now has been the conventional measure that many national aquacultures are taked so daily ration is mended selenium.Current widely used benefit selenium method is to add sodium selenite.But because the sodium selenite utilization rate is low, toxicity is big, and can cause potential pollution to environment, so some countries have limited the nutritious supplementary pharmaceutical of use sodium selenite as selenium.Must use organic selenium as Sweden's regulation piglet feed, and Japan also forbids using inorganic selenium in piglet feed.The U.S. is also controlling inorganic selenium in animal application on one's body.Some studies show that organic selenium has been compared many advantages with inorganic selenium, and at first its toxicity is less, and good palatability is convenient to use in feed.Its absorption rate height of this external cause can reduce the addition of selenium in the feed, thereby reduces the pollution to environment.
In 20th century, the discovery and the chemical antibacterial material of antibiotic, hormone, parahormone etc. are widely used as feed addictive, and they are on Production of Livestock and Poultry, to control, the promotion growth of disease, improve the quantity and the living standards of the people of meat product, played important function.But because to the using for a long time and abusing of these additives, its drawback is familiar with day by day widely, such as promoting antibody-resistant bacterium to increase; Increase the sensitiveness of susceptible biology, promote the exogenous and autogenous infection of body; Reduce the animal immune function, Abwehrkraft des Koepers is weakened; Residual in animal products, threaten human body health; The humans and animals teratogenesis is disabled, even cause humans and animals to be poisoned to death.In order to overcome the drawback that these additives bring, scientist make every effort to develop a kind of safely, have no side effect, noresidue, can promote growth of animal, again can disease preventing and treating, substitute antibiotic new additive agent.Along with the research and the application of bio-additive, the researcher finds that bio-additive not only has the effect of preventing and treating diseases, but also has the animal feed intake of increasing and promote growth, improves the effect of weightening finish.Bio-additive also claims feed with microbe additive or probio (probiotics), have another name called regulator, bacteria-promoting agent, probiotic, probiotics, probiotics, active bacteria formulation etc., be meant " can be to the effective microorganism live body of host health " when taking in when a certain amount of.Probio has the function of the following aspects: prevent enteric infection, keep the gut flora balance; Improve immunity function; The control serum cholesterol; Antitumor action etc.Kind surplus the probio bacterial classification that is applied to animal has at present broken through 40, FDA Food and Drug Administration in 1989 and U.S. feed association have issued directly that the probio bacterial classification of feeding animals has 41 kinds.China Ministry of Agriculture has announced 12 kinds of directly feeding animals in June, 1999, allows the feed grade probio bacterial classification that uses.
After entering WTO, the degree of China's reform and opening-up will further strengthen.Because China's livestock products quality and meat product health level are obvious with external gap, thereby abroad the high-quality livestock products will pour in the domestic market, influence the outlet of domestic livestock products simultaneously, aggravate the quality competition pressure of livestock products.Utilizing probio to transform inorganic selenium is organic selenium, make its product have the double action of organic selenium and probio, obviously ecological environment in the animal body, antagonism pathogenic microorganism be can improve better, immunologic function and the premunition of animal strengthened, be safety, noresidue, pollution-free, alternative antibiotic green feed additive, will playing a significant role aspect development China's animal husbandry and the raising meat product health level.
Scarce selenium is worldwide problem, serious low selenium area, China place, and the whole nation has 72% area to have scarce selenium in varying degrees.By in feed, adding the additive contain selenium-enriched probiotics, both can promote growth of animal, improve animal immune function and premunition, can improve again the animal meat Se content, make rich selenium meat product.Rich selenium meat product for a change in China people diet structure the selenium deficiency nutritional health food is provided, this builds up people's health, protects the needs of people ' s health especially for the people in the scarce selenium area of living in nearly 2/3rds areas in the whole nation.
Three, summary of the invention
Technical problem:
The purpose of this invention is to provide a kind of production technology that contains the feed addictive of selenium-enriched probiotics,, provide and add the additive that contains selenium-enriched probiotics in the feed at scarce selenium problem.This product has the double action of organic selenium and probio, both can promote growth of animal, improve animal immune function and premunition, can improve the Se content of animal meat again.
Technical scheme: a kind of production method that contains the feed addictive of selenium-enriched probiotics comprises:
1) bacterial classification: (L.acidophilus LA) and candida utili (Candida utilis), is the Ministry of Agriculture and allows the feed grade probio bacterial classification that uses lactobacillus acidophilus;
2) culture medium: 0.5%～2% yeast extract, 0.5%～2% glucose, pH nature; 100～120 ℃, high pressure steam sterilization 15min, standby;
3) inorganic selenium source: the preparation selenium content is the sodium selenite solution of 0.5～5mg/ml, 100～125 ℃, and high pressure steam sterilization 30min, standby;
4) acquisition of the activation of bacterial classification and seed liquor
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 20～30 ℃, cultivates 30～40h; After activating 3～5 times so continuously, be inoculated in the seed culture medium, 20～30 ℃, 220r/min, shaking table is cultivated 30～40h, obtains candida utili liquid seeds liquid;
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 30～40 ℃, cultivates 30～40h; After activating 3～5 times so continuously, be inoculated in the seed culture medium, 30～40 ℃, cultivate 30～40h, obtain the lactobacillus acidophilus seed liquor;
5) produce:
Inorganic selenium source is added in the culture medium, make that selenium concentration is 3～6mg/kg in the culture medium; Candida utili and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium by 1: 4, and total inoculum concentration is 2～5%; Cultivation temperature: 20～40 ℃; Throughput: 0.05m
3/ h; Revolution: 70r/min; Tank pressure: 0.04Mpa; Incubation time: 30～40h;
6) organic selenium inversion accelerating agent: the Radix Astragali decocting liquid 0.5ml that when cultivating beginning, adds 1～5g oligosaccharides, 0.002～0.5g methionine, 0.002～0.5g serine, the 0.05～2.5g Radix Astragali/ml in every liter of culture medium;
7) can: the selenium-enriched probiotics nutrient solution with after the wheat bran absorption, 30～40 ℃ of oven dry packing, is obtained containing the feed addictive of selenium-enriched probiotics.
Above-mentioned acquisition contains that the cfu number of candida utili and lactobacillus acidophilus is respectively 10 in the feedstuff additive product of selenium-enriched probiotics
9/ g and 10
11/ g; Selenium content is 50.m/kg, and wherein organic selenium content is more than 90%, and selenomethionine content is more than 75%.
Beneficial effect:
Advantage and the good effect that contains the feed addictive of selenium-enriched probiotics provided by the present invention is:
(1) still do not have the feed addictive that contains selenium-enriched probiotics both at home and abroad, it is organic selenium that this additive utilizes probio to transform inorganic selenium, brings into play the double action of organic selenium and probio, can improve ecological environment in the animal body; The antagonism pathogenic microorganism; Regulate the absorption and the consumption of vitamin; Strengthen immunologic function and the premunition of animal; Regulate the animal reproduction function; Antitumor; Green Tea Extract; The poisonous element of antagonism; Be safety, noresidue, pollution-free, alternative antibiotic green feed additive.
(2) the present invention contains the feed addictive of selenium-enriched probiotics, makes an addition in the domestic animal daily ration, can improve breeding performonce fo animals: average daily gain is significantly increased, and the material anharmonic ratio obviously reduces (table 3); And can regulate organism metabolism; Improve immunity of organisms and premunition.
(3) the present invention contains the feed addictive of selenium-enriched probiotics, make an addition in the domestic animal daily ration, can improve selenium content and the organic selenium content of livestock meat quality and muscle, kidney, liver, selenium content reaches 0.32-0.43mg/kg in the domestic animal muscle, selenium major part contained in the domestic animal muscle is the selenium of organic form, is the high selenium of edible safety, bioavailability (table 6,12).
(4) the present invention contains the feed addictive of selenium-enriched probiotics, makes an addition in the domestic animal daily ration, can improve animal blood, kidney, liver selenium content; Improve animal blood and liver activity of glutathione peroxidase; Reduce mda content; Improve the blood superoxide dismutase activity; The concentration (table 4,5,6,7,8,11,12,13,14) of IL-1, IL-2, IL-6 and TNF-α in hog cholera antibody level and the blood in the raising blood.
Four specific embodiment
Embodiment 1: Yanchi County pig farm, Ningxia
Contain the feed addictive of selenium-enriched probiotics, production method is as follows:
(1) bacterial classification:
(L.acidophilus, LA): preserve the center available from the Institute of Microorganism, Academia Sinica microorganism fungus kind, the bacterial strain code name is AS1.1878 to lactobacillus acidophilus.
Candida utili (Candida utilis): preserve the center available from the Institute of Microorganism, Academia Sinica microorganism fungus kind, the bacterial strain code name is AS2.1180.
(2) culture medium: the 1kg yeast extract, 1kg glucose is dissolved in 100L water, the pH nature; 115 ℃, high pressure steam sterilization 15min, standby.
(3) inorganic selenium source: preparation Se concentration is the sodium selenite solution 500ml of 1mg/ml, 121 ℃, and high pressure steam sterilization 30min, standby.
(4) acquisition of the activation of bacterial classification and seed liquor
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 28 ℃, cultivates 32h; After activating 3～5 times so continuously, be inoculated in the 0.5L seed culture medium, 28 ℃, 220r/min, shaking table is cultivated 32h, obtains candida utili liquid seeds liquid.
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 37 ℃, cultivates 32h; After activating 3～5 times so continuously, be inoculated in the 2L seed culture medium, 37 ℃, cultivate 32h, obtain the lactobacillus acidophilus seed liquor.
(5) produce:
500ml sodium selenite (1mg/ml is in Se) solution is added in the culture medium; Candida utili and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium by 1: 4, and total inoculum concentration is 2.5L; Add organic selenium inversion accelerating agent: add sterilized 200g oligosaccharides, 1 gram methionine, 1 gram serine, the Radix Astragali decocting liquid (50ml of the 0.2g Radix Astragali/ml) in the culture medium; Cultivation temperature: 30 ℃; Throughput: 0.05m
3/ h; Revolution: 70r/min; Tank pressure: 0.04Mpa; Incubation time: 36h; Can: the selenium-enriched probiotics nutrient solution with after the wheat bran absorption, 37 ℃ of oven dry packing, is obtained containing the feed addictive 10kg of selenium-enriched probiotics.
Above-mentioned acquisition contains that the cfu number of candida utili and lactobacillus acidophilus is respectively 2.8 * 10 in the feed addictive of selenium-enriched probiotics
9/ g and 2.1 * 10
11/ g; Selenium content is 50.52mg/kg, and wherein organic selenium content is 91.7%, and selenomethionine content is 75.20%.When using, add selenium-enriched probiotics 6kg in the feed per ton as pig feed additive.
Above-mentioned experimental animal when having added the feed that contains the selenium-enriched probiotics additive and experimentizing is chosen, processing and feeding and management, comprising:
1. select that age in days is approaching, body weight is that health Du * length * Dasanyuan about 15kg is hybridized weanling pig as experimental subjects.
2. basal diet cooperates the mash feed that forms with reference to NRC (1998) piglet nutritional need, and its prescription and trophic level see Table 1.
3. add the additive that contains selenium-enriched probiotics in the daily ration, its dosage is the 0.3mg/kg daily ration in selenium.
4. feed and added before the daily ration of the additive that contains selenium-enriched probiotics, be numbered routinely, work such as epidemic prevention, expelling parasite are good for the stomach.
5. whole experiment totally 60 days, the abundant feeding of experimental session is freely drunk water Routine Management.
6. use the feed addictive that contains selenium-enriched probiotics, make an addition in the piglet diet, can improve the production performance of pig: average daily gain is significantly increased, and the material anharmonic ratio obviously reduces; And can regulate organism metabolism; Improve immunity of organisms and premunition.Can improve the selenium content and the organic selenium content of tame meat quality and muscle, (selenium content reaches 0.32mg/kg in the pig muscle) improves pig blood, kidney, liver selenium content; Improve pig blood and liver activity of glutathione peroxidase; Reduce mda content; Improve the blood superoxide dismutase activity; The concentration (table 1,2,4,5,6,7) of IL-1, IL-2, IL-6 and TNF-α in hog cholera antibody level and the blood in the raising blood.
Table 1 basal diet prescription and trophic level
| Form | Trophic level | ||
| Corn-soybean meal oil cake of flax seed time powder wheat bran dusty yeast vegetable oil calcium monohydrogen phosphate sodium acid carbonate stone flour salt multidimensional microelement pre-mix material lysine hydrochloride | 54.2 22.0 5.0 8.0 6.0 0.5 1.0 1.0 0.2 0.9 0.3 0.2 0.5 0.2 | Digestible energy (MJ/kg) crude protein lysine egg+cystine phosphorus calcium | 13.40 17.84 0.9 0.58 0.60 0.73 |
1)Multidimensional is formed (in every kg feed): VA 2200IU, VD 200IU, VE 30mg, VB
14.4mg, VB
24mg, VB
64mg, VB
1215 μ g, nicotinic acid 22mg, pantothenic acid 9mg, biotin 0.10mg, folic acid 0.35mg, Choline chloride 600mg
2)Microelement pre-mix material is formed (in every kg feed): Cu (CuSO
45H
2O) 18mg, Mn (MnSO
4H
2O) 55mg, Fe (FeSO
47H
2O) 80mg, Zn (ZnSO
47H
2O) 80mg, I (KI) 0.14mg
Table 2 contains the chemical form of selenium in the feed addictive of selenium-enriched probiotics and the content of organic selenium
| Total Se content (μ g/g) | The content of organic selenium (μ g/g) | The content of inorganic selenium (μ g/g) | Selenomethionine content (μ g/g) | The percentage composition of organic selenium (%) | The percentage composition of selenomethionine (%) |
| 50.520 | 46.327 | 4.193 | 37.991 | 91.7 | 75.20 |
The influence of table 3 pair piglet growth performance
n＝8
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Average daily ingestion amount (g) material of starting weight (kg) heavy (kg) average daily gain in end (g) anharmonic ratio | 15.36±1.23 a 52.12±1.45 b 612±33 b 1617±68 a 2.64±0.02 a | 15.52±1.12 a 56.64±1.36 a 685±21 a 1628±48 a 2.38±0.04 b | 15.28±1.24 a 52.31±1.34 b 617±24 b 1619±56 a 2.62±0.03 a |
Annotate: indicate different capitalization differences extremely significantly (P＜0.01) among the colleague, indicate different lowercase differences significantly (P＜0.05).Down together.
Table 4 different selenium resources is to the influence of piglet blood GSH-Px activity and Blood Selenium content
n＝8
| Control group | The selenium-enriched probiotics group | The sodium selenite group | |
| Blood GSH-Px activity (units/L) 0d 30d 60d Blood Selenium content Se (mg/L) 0d 30d 60d | 179.38±6.71 a 107.44±1.09 C 123.51±2.63 C 0.158±0.011 a 0.104±0.006 C 0.112±0.007 C | 181.14±5.25 a 224.73±2.42 Aa 227.49±2.05 Aa 0.157±0.008 a 0.494±0.003 Aa 0.509±0.005 Aa | 181.34±4.20 a 207.68±3.04 B 220.58±4.38 B 0.158±0.010 a 0.273±0.007 B 0.312±0.005 B |
Table 5 different selenium resources is to the influence of piglet activity of SOD in serum and MDA content
n＝8
| Control group | Selenium-enriched probiotics group t | The sodium selenite group | |
| Activity of SOD in serum, (u/ml) 0d 30d 60d Content of MDA, (nmol/ml) 0d 30d 60d | 144.12±12.21 a 134.19±4.75 B 130.36±5.71 C 4.32±0.43 a 4.89±0.35 a 5.23±0.48 a | 145.08±10.11 a 154.26±5.52 Aa 158.95±5.39 Aa 4.41±0.38 a 4.01±0.57 b 3.63±0.54 c | 145.11±8.64 a 151.64±5.16 Aa 146.92±4.48 B 44.34±0.24 a 4.06±0.49 b 3.71±0.58 b |
Table 6 different selenium resources is organized the influence of Se content to piglet
n＝4，mg/kg
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Liver kidney muscle | 0.198±0.012 C 1.174±0.045 C 0.078±0.003 C | 0.549±0.007 Aa 1.961±0.006 Aa 0.315±0.018 Aa | 0.337±0.004 B 1.673±0.035 B 0.156±0.010 B |
Table 7 different selenium resources is to the influence of interleukin 1 in the piglet serum and interleukin 2 concentration
n＝8
| Control group | The selenium-enriched probiotics group | The sodium selenite group | |
| Interleukin 1 (ng/ml) 0d 30d 60d interleukin 2 (ng/ml) 0d 30d 60d | 0.125±0.016 a 0.120±0.011 B 0.098±0.023 B 5.077±0.583 a 4.507±0.186 B 4.193±0.192 B | 0.127±0.012 a 0.205±0.007 Aa 0.212±0.018 Aa 5.104±0.531 a 5.576±0.202 Aa 5.835±0.186 Aa | 0.123±0.021 a 0.206±0.013 Aa 0.210±0.015 Aa 5.078±0.503 a 5.649±0.178 Aa 5.819±0.211 Aa |
Table 8 different selenium resources is to the influence of interleukin-6 in the piglet serum and TNF concentration
n＝10
| Project | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| The sub-ng/ml of interleukin-6 (pg/ml) 0d 30d 60d neoplasm necrosis) 0d 30d 60d | 1157.02±179.20 a 1037.68±122.67 b 980.34±136.89 B 1.048±0.102 a 0.967±0.206 b 0.893±0.134 B | 1165.45±168.32 a 1256.46±161.49 a 1273.08±153.26 Aa 1.053±0.107 a 1.413±0.163 a 1.524±0.158 Aa | 1154.56±179.25 a 1251.84±148.12 a 1275.82±141.23 Aa 1.057±0.105 a 1.408±0.181 a 1.518±0.162 Aa |
Embodiment 2: Ningxia Yanchi County Yang Chang
Contain the feed addictive of selenium-enriched probiotics, production method is the same.
Above-mentioned acquisition contains that the cfu number of candida utili and lactobacillus acidophilus is respectively 10 in the feed addictive of selenium-enriched probiotics
9/ g and 10
11/ g; Selenium content is 48.648mg/kg, and wherein organic selenium content is 92.2%, and selenomethionine content is 78.07%.When using, add selenium-enriched probiotics 2kg in the feed per ton as feed addictive.
Above-mentioned experimental animal when having added the feed that contains the selenium-enriched probiotics additive and experimentizing is chosen, processing and feeding and management, comprising:
1. select that age in days is approaching, body weight is that 40 healthy sheep known for its fine thick wool lambs about 20kg are as experimental subjects.
2. the experimental basis diet is the feed resource situation according to the locality, drafts with reference to the nutritional requirements of fat lamb in the feeding standard of NRC, cooperates the mash feed that forms, and its prescription and trophic level see Table 9.
3. add the additive that contains selenium-enriched probiotics in the daily ration, its dosage is the 0.1mg/kg daily ration in selenium.
4. feed and added before the daily ration of the additive that contains selenium-enriched probiotics, be numbered routinely, work such as epidemic prevention, expelling parasite are good for the stomach.
5. whole experiment totally 60 days, the abundant feeding of experimental session is freely drunk water Routine Management.
6. use the feed addictive that contains selenium-enriched probiotics, make an addition in the lamb daily ration, can improve the production performance of sheep: average daily gain is significantly increased, and the material anharmonic ratio obviously reduces; And can regulate organism metabolism; Improve immunity of organisms and premunition.Can improve the selenium content and the organic selenium content of quality of mutton and muscle, (selenium content reaches 0.43mg/kg in the sheep muscle) improves sheep blood, kidney, liver selenium content; Improve sheep blood and liver activity of glutathione peroxidase; Reduce mda content; Improve blood superoxide dismutase activity (table 10,11,12,13,14).
Table 9 basal diet prescription and trophic level
％
| Form Ingredients | Trophic level Nutrient level | ||
| Corn oil cake of flax seed wheat bran alfalfa meal calcium carbonate salt compound additive | 56.0 20.0 10.0 13.0 0.3 0.4 0.3 | Digestible energy (MJ/kg) crude protein crude fibre phosphorus calcium | 13.39 18.5 6.2 0.87 1.25 |
Table 10 contains the chemical form of selenium in the feed addictive of selenium-enriched probiotics and the content of organic selenium
| Total Se content (μ g/g) | Organic selenium content (μ g/g) | Inorganic selenium content (μ g/g) | The content of selenomethionine (μ g/g) | The percentage composition of organic selenium (%) | The percentage composition of selenomethionine (%) |
| 48.648 | 44.853 | 3.795 | 37.979 | 92.2 | 78.07％ |
The influence of table 11 pair lamb growth performance
n＝10
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Average daily ingestion amount (g) material of starting weight (kg) heavy (kg) average daily gain in end (g) anharmonic ratio | 20.9±2.71 a 29.42±2.45 b 142±39 b 509±29 a 3.58±0.02 a | 21.7±1.61 a 32.6±3.36 a 172±41 a 512±38 a 2.97±0.04 b | 21.45±2.83 a 30.21±2.04 b 148±34 b 514±26 a 3.47±0.03 a |
Annotate: indicate different capitalization differences extremely significantly (P＜0.01) among the colleague, indicate different lowercase differences significantly (P＜0.05).Down together.
Table 12 different selenium resources is organized the influence of Se content to lamb
n＝5，mg/kg
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Liver kidney muscle | 0.332±0.024 C 1.023±0.015 C 0.086±0.005 | 0.758±0.018 Aa 1.832±0.014 Aa 0.435±0.011 Aa | 0.439±0.016 B 1.425±0.010 B 0.148±0.013 B |
Table 13 different selenium resources is to the influence of lamb blood GSH-Px activity and Blood Selenium content
n＝10
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Blood GSH-Px activity (units/L) 0d 30d 60d Blood Selenium content (mg/L) 0d 30d 60d | 352.78±11.42 a 215.64±5.48 C 244.56±13.07 c 0.110±0.009 a 0.072±0.008 D 0.087±0.003 C | 355.73±11.06 a 507.43±3.96 Aa 467.39±11.77 Aa 0.104±0.011 a 0.375±0.015 A 0.395±0.011 Aa | 355.16±11.66 a 499.60±5.11 B 402.53±14.82 B 0.106±0.015 a 0.268±0.004 C 0.290±0.012 B |
Table 14 different selenium resources is to the influence of lamb activity of SOD in serum and MDA content
n＝10
| Group | Control group | The selenium-enriched probiotics group | The sodium selenite group |
| Activity of SOD in serum, (u/ml) 0d 30d 60d Content of MDA, (nmol/ml) 0d 30d 60d | 97.23±8.48 a 90.56±12.14 B 85.36±9.21 C 6.93±1.13 a 8.85±0.45 A 9.46±0.52 A | 98.26±7.42 a 220.88±18.84 Aa 228.14±12.32 Aa 6.84±1.18 a 4.45±0.34 B 2.04±0.31 Bb | 97.96±7.45 a 221.76±13.56 Aa 231.53±10.16 Aa 6.87±1.36 a 4.25±0.71 B 2.59±0.23 Ba |
Claims (2)
1, a kind of production method that contains the feed addictive of selenium-enriched probiotics comprises:
1) bacterial classification: lactobacillus acidophilus and candida utili;
2) culture medium: 0.5%～2% yeast extract, 0.5%～2% glucose, pH nature; 100～120 ℃, high pressure steam sterilization 15min, standby;
3) inorganic selenium source: the preparation selenium content is the sodium selenite solution of 0.5～5mg/ml, 100～125 ℃, and high pressure steam sterilization 30min, standby;
4) acquisition of the activation of bacterial classification and seed liquor
The candida utili that is kept at 4 ℃ is inoculated on the slant medium, 20～30 ℃, cultivates 30～40h; After activating 3～5 times so continuously, be inoculated in the seed culture medium, 20～30 ℃, 220r/min, shaking table is cultivated 30～40h, obtains candida utili liquid seeds liquid;
The lactobacillus acidophilus that preserves is inoculated on the slant medium, 30～40 ℃, cultivates 30～40h; After activating 3～5 times so continuously, be inoculated in the seed culture medium, 30～40 ℃, cultivate 30～40h, obtain the lactobacillus acidophilus seed liquor;
5) produce:
Inorganic selenium source is added in the culture medium, make that selenium concentration is 3～6mg/kg in the culture medium; Candida utili and lactobacillus acidophilus seed liquor inoculative proportion were inoculated in the culture medium by 1: 4, and total inoculum concentration is 2～5%; Cultivation temperature: 20～40 ℃; Throughput: 0.05m
3/ h; Revolution: 70r/min; Tank pressure: 0.04Mpa; Incubation time: 30～40h;
6) organic selenium inversion accelerating agent: the Radix Astragali decocting liquid 0.5ml that when cultivating beginning, adds 1～5g oligosaccharides, 0.002～0.5g methionine, 0.002～0.5g serine, the 0.05～2.5g Radix Astragali/ml in every liter of culture medium;
7) can: the selenium-enriched probiotics nutrient solution with after the wheat bran absorption, 30～40 ℃ of oven dry packing, is obtained containing the feed addictive of selenium-enriched probiotics.
2, the feed addictive that contains selenium-enriched probiotics that obtains with the described method for producing feed additive that contains selenium-enriched probiotics of claim 1.
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