CN1274199C - Tomato Meloidogyne incogita Chitwood prevention and cure method - Google Patents
Tomato Meloidogyne incogita Chitwood prevention and cure method Download PDFInfo
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- CN1274199C CN1274199C CN 200410039231 CN200410039231A CN1274199C CN 1274199 C CN1274199 C CN 1274199C CN 200410039231 CN200410039231 CN 200410039231 CN 200410039231 A CN200410039231 A CN 200410039231A CN 1274199 C CN1274199 C CN 1274199C
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Abstract
The present invention discloses a method for preventing and curing tomato root-knot nematodes, which aims at providing a method for replacing Methyl bromide to prevent and cure tomato root-knot nematodes effectively. The method for preventing and curing tomato root-knot nematodes provided by the present invention comprises the following steps: 1) accelerating sprouts for seeds of tomato resistance stocks SIS-1 (Lycopersicon lycopersicum *L. hirsutum) at the humidity of 90 to 95%, and at a temperature of 20 DEG C to 26 DEG C for 2 to 4 days; then sowing and cultivating a stock to have 3 to 5 euphyllas; 2) cultivating tomato scion seeds to have 2 to 4 euphyllas; 3) reserving 2 cotyledons for the stock with 3 to 5 euphyllas in step 1); reserving 2 to 3 euphyllas for the scion stock in step 2) to carry out grafting; cultivating the grafted euphyllas to obtain tomato grafted seedlings resistant to root-knot nematodes. The method for preventing and curing tomato root-knot nematodes by tomato resistance stock grafting in the present invention is simple, practical, safe and effective; the present invention not only can prevent environmental pollution caused by using pesticide, but also can improve the quality of products, which is favorable for exporting agricultural products to earn foreign currency, has very high popularization value and has an extensive market outlook.
Description
Technical field
The present invention relates to a kind of method of preventing and treating root knot nematode in the crop resistance diseases prevention technical field, particularly a kind of method of preventing and treating tomato root-knot eelworm.
Background technology
In recent years, China's vegetable protecting field development has reached 2,000,000 hectares rapidly.But because its special working condition provides suitable environment for soil-borne diseases such as root-knot nematodes; the harm of China main vegetable protecting field producing region root-knot nematode is very serious at present; the characteristics that the outburst of reunion formula is arranged into; the economic loss that causes is generally 20%-60%, has seriously restricted China protection ground vegetables and has produced and city " vegetable basket " construction.
The method of the current world and state inner control root-knot nematode mainly concentrates on to be utilized on the chemical pesticide diseases prevention technical elements, the general generation of adopting the method control soil-borne disease of soil-fumigating, as use the sterilization of methyl bromide soil-fumigating, in soil, use the nematode killing agent metham-sodium.In addition, the crop rotation method that adopts the non-host crop of plantation is arranged also, reach the purpose that alleviates the generation of root-knot nematode disease and the extent of injury.Therefore but methyl bromide is not only a kind of extremely toxic substance, can also consume the ozone layer in the atmosphere, in the world the application of methyl bromide is limited.According to Montreal protocol in 1997, developed country will stop using methyl bromide, the Chinese government also to make the formal commitment in 2005 comprehensively and cut down 20%, the thoroughly superseded methyl bromide by 2015 to 2005.Therefore, from the angle that government pays close attention to, China must quicken one's step, and studies simple and effective, economically viable methyl bromide substitute technology, and the loss that effort will cut down and stop using methyl bromide to bring is reduced to minimum degree.Take place though crop rotation can alleviate the root-knot nematode disease, can not reach radical cure, and cost can improve for protection ground.
The innovation and creation content
The method that the purpose of this invention is to provide a kind of effective control tomato root-knot eelworm.
The method of control tomato root-knot eelworm provided by the present invention may further comprise the steps:
1) with tomato resistance stock SIS-1 (seed of Lycopersicon lycopersicum * L.hirsutum) is at humidity 90-95%, temperature 20-26 ℃ vernalization 2-4 days, sow and be cultured to 3-5 sheet true leaf then;
2) the tomato ingraftment seed is cultured to 2-4 sheet true leaf;
3) the stock strain that has 3-5 sheet true leaf described in the step 1) is kept two cotyledons, with step 2) described in the scion strain keep 2-3 sheet true leaf and carry out grafting, cultivate the tomato grafting that obtains anti-root-knot nematode.
Wherein, (seed of Lycopersicon lycopersicum * L.hirsutum) is available from gondola Nuovo Centro.S.E.I.A.Sp.A. company for the resistance of tomato described in step 1) stock SIS-1; (it is 3 days that the seed of Lycopersicon lycopersicum * L.hirsutum) carries out the time of vernalization to described tomato resistance stock SIS-1.
Step 2) the tomato scion in can be existing tomato variety, is preferably mao powder 802; Can according to the suitable growth condition of each kind with its by seed culture to 2-4 sheet true leaf; Also can be according to above-mentioned steps 1) condition cultivate the scion that obtains 2-4 sheet true leaf.
Seedling in the step 3) after stock and the scion grafting can be earlier is that 25-29 ℃, humidity are to cultivate 2-5 days in the environment of 80%-90% in temperature, can not water during this to promote scion and the intact fusion of stock, and then making it slowly adapt to greenhouse experiment in the seedling immigration greenhouse, can carry out normal management after 10 days, can transplant after 15 days.
Experimental results show that, method of the present invention can significantly improve stock seed germination rate (up to 65%) and grafting survival rate (up to 90%), the grafting that obtains has remarkable prevention effect in the field to root knot nematode disease, the disease index average is 17.8, seedling is then up to 80.6, have remarkable economical, society and ecological benefits, established solid foundation for further applying.
The present invention has effectively guaranteed the germination rate of seed, again because rootstock seedling and scion seedling growth rate difference separately, by the control sowing time difference, make rootstock seedling and scion seedling when compatibility is the highest, carry out grafting, promoting the fusion of the two, and by improving breeding condition, the utmost point has improved the grafting survival rate significantly, after it is transplanted, all demonstrate strong anti-root-knot nematode characteristic in good time, reached the volume increase double effects of significantly guaranteeing the quality simultaneously in the whole growth phase.In addition, resistance grafting plant of the present invention can not only root-knot nematode resistant, also other soil-borne diseases are had certain resistance, as the lethality of grafting is starkly lower than the scion seedling after transplanting, and adverse environmental factors such as low temperature, waterlogging, freeze injury are had very strong resistance.The method of tomato resistance stock grafting control root-knot nematode of the present invention is simple and practical, safe and effective, both can prevent that agricultural chemicals from using the environmental pollution that brings, can improve product quality again, help the foreign exchange earning of agricultural product, have very high promotional value, have vast market prospect, can substitute methyl bromide fully in actual applications, reach the purpose of control root-knot nematode.
Embodiment
The cultivation of the tomato grafting of embodiment 1, anti-root-knot nematode
1, the cultivation of the tomato grafting of anti-root-knot nematode
1) presprouting of seeds: keep indoor humidity 90-95%, temperature 20-26 ℃, tomato resistance stock SIS-1 (seed of Lycopersicon lycopersicum * L.hirsutum) or 802 presproutings of seeds of scion hair powder 3 days, then it is seeded in seedbed (matrix components: the 60% black peat composed of rotten mosses, 30% perlite, 10% vermiculite).
Wherein, the sowing time difference is: carried out ingraftment seed vernalization on the 7th day behind the stock planting seed, and sowing in the 10th day, condition of culture is identical with the stock seed.
2) seedling management: after seedling grows, keep 20-30 ℃ of indoor temperature, and often water, impose foliage fertilizer weekly 1 time to keep the seedbed adequate water.Grafting is fully watered, is loosened the soil the previous day, is convenient to transplant seedlings.
3) grafting: arrive 3-5 sheet true leaf when stock is long, when scion length arrives 2-4 sheet true leaf, with sharp blade cut sth. askew down along 45 in the top of two cotyledons of stock, the scion strain is kept 2-3 sheet true leaf from top to bottom cuts sth. askew down with angle, the stock otch is alignd with the scion otch, clip with the grafting exclusive clamps, to promote two-part fusion, grafting is finished.
4) grafting management: it is that 25-29 ℃, humidity are indoor 3 days more than 80% that the seedling after the grafting must be placed on temperature, can not water during this to promote scion and the intact fusion of stock, seedling being moved in the greenhouse after 3 days makes it slowly adapt to greenhouse experiment again, but noon is when temperature is higher, should cover the sunshade net, by the time temperature is opened net after reducing at dusk, can carry out normal management after 10 days, can transplant after 15 days.
5) transplant: the greenhouse temperature of transplanting is preferably about 28 ℃.
The stock seed is relatively expensive, and its characteristics are exactly germination rate not high (being generally 60%), and step 1 control indoor humidity is 90-95%, in the time of temperature maintenance 20-26 ℃, has improved the germination rate of stock seed, reaches desirable 65%; During grafting, the 7th day sowing ingraftment seed behind sowing stock seed, the straight stem size of the most of seedling of the two is all very approaching during grafting, this moment the two amalgamation height, the grafting survival rate reaches 87.0%; Be transplanted into that the whole growth phase all shows extremely strong anti-root-knot nematode ability under the field condition behind the canopy.
2, the evaluation of the anti-root-knot nematode ability of the tomato grafting of anti-root-knot nematode
Hair powder 802 seedlings and the grafting in the step 1 of identical seedling age are transplanted the multiple plot of the root knot nematode disease of testing the base in Qingzhou palace of Hsi Wang Mu town, in identical environment and condition of culture, be cultured to fruit maturation, carry out field investigation, show the whole plant morbidities of mao powder 802 seedlings, and 92% grafting plant normal growth, have only 8% morbidity, guaranteed the high yield and high quality of tomato conscientiously.
Claims (3)
1, a kind of method of preventing and treating tomato root-knot eelworm may further comprise the steps:
1) with Lycopersicon lycopersicum * L.hirsutum tomato resistance stock SIS-1 seed at humidity 90-95%, under the temperature 20-26 ℃ of condition vernalization 2-4 days, sow and be cultured to 3-5 sheet true leaf then;
2) behind the tomato resistance stock SIS-1 planting seed the 7th day, 2-4 sheet true leaf is sowed and be cultured to the tomato ingraftment seed at humidity 90-95%, under the temperature 20-26C condition vernalization 2-4 days, then;
3) the stock strain that has 3-5 sheet true leaf described in the step 1) is kept two true leaves, with step 2) described in the scion strain keep 2-3 sheet true leaf and carry out grafting, seedling after stock and the scion grafting is that 25-29 ℃, humidity are to cultivate 2-5 days in the environment of 80%-90% in temperature earlier, continues to cultivate the tomato grafting that obtains anti-root-knot nematode.
2, method according to claim 1 is characterized in that: the time that the Lycopersiconlycopersicum in the described step 1) * L.hirsutum tomato resistance stock SIS-1 seed carries out vernalization is 3 days.
3, method according to claim 1 and 2 is characterized in that: the tomato scion described step 2) is hair powder 802.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410039231 CN1274199C (en) | 2004-02-09 | 2004-02-09 | Tomato Meloidogyne incogita Chitwood prevention and cure method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410039231 CN1274199C (en) | 2004-02-09 | 2004-02-09 | Tomato Meloidogyne incogita Chitwood prevention and cure method |
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| Publication Number | Publication Date |
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| CN1557125A CN1557125A (en) | 2004-12-29 |
| CN1274199C true CN1274199C (en) | 2006-09-13 |
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| CN 200410039231 Expired - Fee Related CN1274199C (en) | 2004-02-09 | 2004-02-09 | Tomato Meloidogyne incogita Chitwood prevention and cure method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100576996C (en) * | 2007-11-20 | 2010-01-06 | 丁加刚 | Method for cultivating tomato stock using resistant variety axillary buds cuttage |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106905034A (en) * | 2017-01-22 | 2017-06-30 | 福建省热带作物科学研究所 | Improve the fertilizer and method of Tomato Grafting shoot survival percent |
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2004
- 2004-02-09 CN CN 200410039231 patent/CN1274199C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100576996C (en) * | 2007-11-20 | 2010-01-06 | 丁加刚 | Method for cultivating tomato stock using resistant variety axillary buds cuttage |
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| CN1557125A (en) | 2004-12-29 |
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