CN1268746C - 用于在真菌细胞中生产多肽的方法 - Google Patents

用于在真菌细胞中生产多肽的方法 Download PDF

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Publication number
CN1268746C
CN1268746C CNB008045062A CN00804506A CN1268746C CN 1268746 C CN1268746 C CN 1268746C CN B008045062 A CNB008045062 A CN B008045062A CN 00804506 A CN00804506 A CN 00804506A CN 1268746 C CN1268746 C CN 1268746C
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gene
leu
polypeptide
cell
enzyme
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Expired - Fee Related
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CNB008045062A
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English (en)
Chinese (zh)
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CN1342204A (zh
Inventor
D·S·雅弗尔
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Novozymes AS
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Novozymes AS
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Publication of CN1342204A publication Critical patent/CN1342204A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CNB008045062A 1999-02-02 2000-02-02 用于在真菌细胞中生产多肽的方法 Expired - Fee Related CN1268746C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24195599A 1999-02-02 1999-02-02
US09/241,955 1999-02-02

Publications (2)

Publication Number Publication Date
CN1342204A CN1342204A (zh) 2002-03-27
CN1268746C true CN1268746C (zh) 2006-08-09

Family

ID=22912879

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB008045062A Expired - Fee Related CN1268746C (zh) 1999-02-02 2000-02-02 用于在真菌细胞中生产多肽的方法

Country Status (5)

Country Link
EP (1) EP1151106A2 (fr)
JP (1) JP2002535990A (fr)
CN (1) CN1268746C (fr)
AU (1) AU2755300A (fr)
WO (1) WO2000046375A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164574B1 (es) * 1999-12-10 2003-05-16 Consejo Superior Investigacion Mejora de la produccion de penicilina mediante la utilizacion de cepas transgenicas merodiploides para un gen regulador.
EP2102366A4 (fr) * 2006-12-10 2010-01-27 Dyadic International Inc Expression et criblage a haut debit de bibliotheques d'adn exprime complexes dans les champignons filamenteux
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
RU2645252C2 (ru) 2012-01-05 2018-02-19 Глюкос Финланд Ой Клетка нитчатых грибов с дефицитом протеаз и способы ее применения
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
CA2916905A1 (fr) 2013-07-10 2015-01-15 Novartis Ag Cellules fongiques filamenteuses deficientes en plusieurs proteases et procedes d'utilisation de celles-ci
KR101508353B1 (ko) * 2013-08-30 2015-04-06 대상 주식회사 고활성 셀룰라아제를 생산하는 트리코데르마 레세이 변이주 및 그의 용도
FR3018522B1 (fr) * 2014-03-17 2018-02-02 IFP Energies Nouvelles Souches mutantes de trichoderma reesei
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
JP6473339B2 (ja) * 2015-02-05 2019-02-20 本田技研工業株式会社 麹菌変異株及び形質転換体
KR101751966B1 (ko) 2015-06-23 2017-06-30 대상 주식회사 트리코데르마 속 균주의 혼합배양에 의한 고활성 셀룰라아제를 생산하는 방법
CN114786473B (zh) * 2019-10-10 2023-12-19 嘉士伯有限公司 制备突变植物的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0966521A1 (fr) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cellules presentant des mutations d'insertion d'adn produisant des quantites modifiees d'un polypeptide
AU4213999A (en) * 1998-05-27 1999-12-13 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene

Also Published As

Publication number Publication date
WO2000046375A2 (fr) 2000-08-10
EP1151106A2 (fr) 2001-11-07
JP2002535990A (ja) 2002-10-29
WO2000046375A3 (fr) 2000-11-23
AU2755300A (en) 2000-08-25
CN1342204A (zh) 2002-03-27

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Patentee before: Novozymes Biotech Inc.

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