CN1268746C - 用于在真菌细胞中生产多肽的方法 - Google Patents

用于在真菌细胞中生产多肽的方法 Download PDF

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Publication number
CN1268746C
CN1268746C CNB008045062A CN00804506A CN1268746C CN 1268746 C CN1268746 C CN 1268746C CN B008045062 A CNB008045062 A CN B008045062A CN 00804506 A CN00804506 A CN 00804506A CN 1268746 C CN1268746 C CN 1268746C
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China
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gene
leu
polypeptide
cell
enzyme
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Expired - Fee Related
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CNB008045062A
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English (en)
Chinese (zh)
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CN1342204A (zh
Inventor
D·S·雅弗尔
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Novozymes AS
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Novozymes AS
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Publication of CN1342204A publication Critical patent/CN1342204A/zh
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Publication of CN1268746C publication Critical patent/CN1268746C/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CNB008045062A 1999-02-02 2000-02-02 用于在真菌细胞中生产多肽的方法 Expired - Fee Related CN1268746C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24195599A 1999-02-02 1999-02-02
US09/241,955 1999-02-02

Publications (2)

Publication Number Publication Date
CN1342204A CN1342204A (zh) 2002-03-27
CN1268746C true CN1268746C (zh) 2006-08-09

Family

ID=22912879

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB008045062A Expired - Fee Related CN1268746C (zh) 1999-02-02 2000-02-02 用于在真菌细胞中生产多肽的方法

Country Status (5)

Country Link
EP (1) EP1151106A2 (de)
JP (1) JP2002535990A (de)
CN (1) CN1268746C (de)
AU (1) AU2755300A (de)
WO (1) WO2000046375A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164574B1 (es) * 1999-12-10 2003-05-16 Consejo Superior Investigacion Mejora de la produccion de penicilina mediante la utilizacion de cepas transgenicas merodiploides para un gen regulador.
EP2505651A3 (de) * 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
WO2013048661A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
JP6245528B2 (ja) 2012-01-05 2017-12-13 グリコス・フィンランド・オーワイＧｌｙｋｏｓ Ｆｉｎｌａｎｄ Ｏｙ プロテアーゼ欠損糸状菌細胞及びその使用方法
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
CN105980552A (zh) 2013-07-10 2016-09-28 诺华股份有限公司 多种蛋白酶缺陷的丝状真菌细胞及其使用方法
KR101508353B1 (ko) * 2013-08-30 2015-04-06 대상 주식회사 고활성 셀룰라아제를 생산하는 트리코데르마 레세이 변이주 및 그의 용도
FR3018522B1 (fr) * 2014-03-17 2018-02-02 IFP Energies Nouvelles Souches mutantes de trichoderma reesei
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
JP6473339B2 (ja) 2015-02-05 2019-02-20 本田技研工業株式会社 麹菌変異株及び形質転換体
KR101751966B1 (ko) 2015-06-23 2017-06-30 대상 주식회사 트리코데르마 속 균주의 혼합배양에 의한 고활성 셀룰라아제를 생산하는 방법
MX2022004361A (es) * 2019-10-10 2022-05-03 Carlsberg As Metodos para preparar plantas mutantes.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958727A (en) * 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
CN1307642A (zh) * 1998-05-27 2001-08-08 诺沃诺尔迪斯克生物技术有限公司 通过改变基因拷贝数制备多肽的方法

Also Published As

Publication number Publication date
AU2755300A (en) 2000-08-25
JP2002535990A (ja) 2002-10-29
EP1151106A2 (de) 2001-11-07
WO2000046375A3 (en) 2000-11-23
WO2000046375A2 (en) 2000-08-10
CN1342204A (zh) 2002-03-27

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Patentee before: Novozymes Biotech Inc.

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