CN1266270C - Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte - Google Patents
Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte Download PDFInfo
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- CN1266270C CN1266270C CN 200410012306 CN200410012306A CN1266270C CN 1266270 C CN1266270 C CN 1266270C CN 200410012306 CN200410012306 CN 200410012306 CN 200410012306 A CN200410012306 A CN 200410012306A CN 1266270 C CN1266270 C CN 1266270C
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Abstract
The present invention discloses a method and a device for preparing washed red blood cells and freezing-unfreezing glycerol removing red blood cells. A blood collection bag of which the bottom is provided with a filter is hung on a hanging hook of an operation cabinet; during the operation of a combination liquid filler, the combination liquid filler is hung on a hanging hook arranged in the uppermost layer of the operation cabinet and is positioned above the blood collection bag, and liquid is added to the blood collection bag under the gravity action; a negative pressure sealed box connected with a vacuum pump is placed in the lowest layer of the operation cabinet and is positioned below the blood collection bag, and a plasma bag and a waste liquid bag are arranged in the negative pressure sealed box; and the sealed box is connected with the vacuum pump so that the inner part of the sealed box is in a negative pressure state. Switches of the blood collection bag and a combination occasion bag are opened to quickly filter blood so as to separate plasma and blood cells from the blood and carry out washing and the like, and washed red blood cells and freezing-unfreezing glycerol removing red blood cells can be prepared. The present invention solves the problems of easy pollution and short storage life as a result of repeated washing existing in the past technical operation; the present invention is favorable to the further centralized management of blood collection and blood supply and suitable for hospital blood banks and blood stations to use.
Description
Technical field
The present invention relates to the method and the device of a kind of preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized, belong to the preparing technical field of blood separation and goods thereof.
Background technology
At present, processes such as separated plasma and washing hemocyte all adopt centrifugal back siphon or plasma-separating clip extruding to remove methods such as supernatant liquor, cause the pollution and the loss of hemocyte easily, required equipment and environmental requirement strictness, be not suitable in hospital blood bank, operating, Washed Red Blood Cells cost height with this method preparation, preservation period is very short, the preparation Red Blood Cells Frozen and Deglycerolized needs centrifugal and carrying out washing treatment process repeatedly, the easier pollution, preservation period is shorter, has seriously limited the clinical application of Washed Red Blood Cells and Red Blood Cells Frozen and Deglycerolized blood products.
Summary of the invention
The object of the present invention is to provide the method for a kind of preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized, adopt the negative pressure leaching mode, blood is carried out suction filtration separate and carrying out washing treatment, overcome conventional art and polluted easily and short defective of red corpuscle shelf lives.
The present invention also aims to provide a kind of device that is used for preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized.
Design of the present invention is such.Use has the special-purpose blood taking bag of strainer, under aseptic situation, gather blood, plasma bags and waste fluid bag are placed in the negative-pressure sealing case, the negative-pressure sealing case is connected with vacuum pump, make in plasma bags and the waste fluid bag and be in negative pressure state, blood is carried out suction filtration separation and washing fast, blood taking bag adopts disposable sterilized apyrogenic plastics, closed-loop operation, the time is short, can reduce the waste and the pollution of hemocyte, required equipment and operative technique are simple, be convenient to carry out in blood station and hospital blood bank, directly very low temperature is freezing in the blood station for frozen red cells, stores in hospital blood bank or freezing transportation down; After frozen red cells intensification speed is melted, wash, go glycerine to carry out under negative pressure equally, can obtain the thawing to reduce glycerol red corpuscle after the washing, perfusion physiological saline can be transfused blood.
Particularly, method of the present invention may further comprise the steps:
(1) preparing washing red corpuscle and frozen red cells
(1) use the blood taking bag that has strainer under aseptic situation, to gather blood;
(2) with blood taking bag with have the composite reagent bag that makes up liquid fillers and be connected, the composite reagent bag is positioned at the top of blood taking bag;
(3) plasma bags and waste fluid bag are put into the negative-pressure sealing case, close the negative-pressure sealing case, plasma bags is connected with the leakage fluid dram of blood taking bag with waste fluid bag, the negative-pressure sealing case is placed on the below of blood taking bag, and is connected with vacuum pump;
(4) open vacuum pump, make in the negative-pressure sealing case to be negative pressure state;
(5) be under the negative pressure state in plasma bags and the waste fluid bag, in blood taking bag, add corresponding reagent successively, blood is carried out suction filtration, washing and sterilization, emit blood plasma and waste liquid, make Washed Red Blood Cells; Or in blood taking bag, add glycerine preservation liquid, in order to preserving;
(6) take off blood taking bag, it is preserved at-80 ℃ of very low temperature, promptly get frozen red cells.
(2) preparation thawing to reduce glycerol red corpuscle (equipment therefor is identical with the preparing washing red corpuscle)
(1) takes out the blood taking bag that glycerine preservation liquid is housed, put into 37 ℃ of constant water bath box, make its thawing;
(2) connect and have composite reagent bag and the negative-pressure sealing case that makes up liquid fillers, 9% sodium chloride solution, 6% hydroxyethyl starch solution, 0.9% sodium chloride solution are housed respectively in the composite reagent bag and contain the aseptic apyrogenic physiological saline of 1% hydrogen peroxide, waste fluid bag is put into the negative-pressure sealing case, close the negative-pressure sealing case, the extraction pipe of negative-pressure sealing case is connected with vacuum pump;
(3) the composite reagent bag is preserved the blood taking bag fluid inlet of liquid and linked to each other with glycerine is housed, the liquid-inlet pipe of the leakage fluid dram of the blood taking bag waste fluid bag interior with being placed on the negative-pressure sealing case is connected;
(4) open vacuum pump, make the waste fluid bag in the negative-pressure sealing case be in negative pressure state, elder generation's suction filtration goes out glycerine and preserves liquid, wash the 1st time with 9% sodium chloride solution and 6% hydroxyethyl starch solution, wash the 2nd time with 6% hydroxyethyl starch solution and 0.9% sodium chloride solution again, after washing is finished, directly pour into the aseptic apyrogenic physiological saline that contains 1% hydrogen peroxide of equivalent, make the thawing to reduce glycerol red corpuscle.
Method of the present invention is used and is finished with lower device.Comprise in its structure and have the composite reagent bag that makes up liquid fillers, the blood taking bag that the bottom has strainer, the composite reagent bag is above blood taking bag, the below of blood taking bag is the negative-pressure sealing case, interior dress plasma bags and waste fluid bag, the extraction pipe of negative-pressure sealing case links to each other with vacuum pump, the liquid adding bottle of combination liquid fillers is connected with the fluid inlet of composite reagent bag, blood taking bag respectively, and the plasma bags that the leakage fluid dram of blood taking bag and negative-pressure sealing case are interior and the liquid-inlet pipe of waste fluid bag are connected.
Said apparatus has cabinet type structure, having the composite reagent bag (bottle) that makes up liquid fillers hangs on the superiors' hook of operating cabinet, by action of gravity blood taking bag is carried out liquid feeding, the negative-pressure sealing case that links to each other with vacuum pump is placed on the orlop of operation cabinet, vacuum pump is opened in the below of blood taking bag, and making the plasma bags and the waste fluid bag that are in the negative-pressure sealing case is negative pressure state, turn on the respective switch of combination liquid fillers and blood taking bag, can finish the suction filtration to blood, the operation of washing and going glycerine preservation liquid.
The technical progress that the present invention obtains is: present method and device be separated plasma and hemocyte rapidly, and hemocyte is carried out carrying out washing treatment, and the suitable most patients clinically of the blood cell suspension of preparation is directly used; The present invention can prepare the red cell suspension of leucocyte-removing specially, and especially Red Blood Cells Frozen and Deglycerolized of Washed Red Blood Cells helps promoteing the wider application; Frozen red cells prolonged in the following storage life of freezing state, can reach 10 years, was longer than under 4 ℃ of conditions 21 days or 35 days preservation perives of hemocyte far away; Can avoid the waste of blood resource; In the very low temperature refrigerator-freezer of blood station, can store in a large number or under freezing state, transport, help realizing the centralized management of blood collecting and supplying, can be every some months, 1 year even longer time, the blood station is transported blood products according to predetermined quantity one time to hospital blood bank; Equipment therefor is simple in structure, suitable, easy to operate, is convenient to apply in hospital blood bank.
Description of drawings
Fig. 1 is used for the erythrocytic device synoptic diagram of the inventive method preparing washing.
Fig. 2 is used for method of the present invention to prepare the erythrocytic device synoptic diagram of thawing to reduce glycerol.
Fig. 3 is the filter membrane synoptic diagram of strainer.
Fig. 4 is the filter core and the support synoptic diagram of strainer.
Fig. 5 is the sealing-ring synoptic diagram of strainer.
Fig. 6 is the outrigger synoptic diagram of strainer.
Need to prove that apparatus structure illustrated in figures 1 and 2 is identical, there is no essential distinction.
The implication of each label representative is as follows among the figure:
1, operation cabinet 2, composite reagent bag hook 3-1,3-2,3-3,3-4, composite reagent bag 4-1,4-2,4-3,4-4,4-5 makes up liquid fillers 5, the blood taking bag 6 of band strainer, membrane filter 7-1, blood taking bag fluid inlet 7-2,7-3,7-4, blood taking bag leakage fluid dram 8,8-1,8-2, plasma bags, waste fluid bag liquid-inlet pipe 9, negative-pressure sealing case 10, negative-pressure sealing box cover 11, negative-pressure sealing case vapor pipe 12, extraction pipe 13, vacuum pump 14-1,14-2,14-3,14-4, plasma bags, waste fluid bag.
Embodiment
Following with reference to drawings and Examples explanation the present invention.
Embodiment 1
Present embodiment is narrated with reference to accompanying drawing.
As shown in Figure 1, the erythrocytic method equipment therefor of preparing washing, the bottom of blood taking bag 5 has membrane filter 6, circular filter membrane by the band micropore, circular seal ring, the filter core and the circular filter of band sieve aperture are configured to outward, the filter membrane of band micropore is placed on above the filter core of band sieve aperture, seal by sealing-ring, add a cover the strainer outrigger above, whole strainer is fixed in the bottom of blood taking bag by support, the filter core of band sieve aperture is communicated with the leakage fluid dram of blood taking bag and is fastenedly connected, have combination liquid fillers 4-1,4-2,4-3, the composite reagent bag 3-1 of 4-4,3-2,3-3,3-4 hangs on the superiors' hook 2 of operation cabinet 1, be positioned at the top of blood taking bag 5, by action of gravity blood taking bag 5 is carried out liquid feeding, the combination liquid fillers is the five-way pipe support, the pipe 4-1 that wherein has switch, 4-2,4-3,4-4 is connected with the composite reagent bag respectively, pipe 4-5 is connected with the fluid inlet 7-1 of blood taking bag 5, the negative-pressure sealing case 9 that links to each other with vacuum pump 13 is placed on the orlop of operation cabinet, the below of blood taking bag 5, interior dress plasma bags and waste fluid bag 14-1,14-2,14-3,14-4.The erythrocytic operating process of preparing washing is: before blood sampling, at first add the blood Precerving liquid in the blood taking bag 5 according to blood sampling volume, select the blood taking bag of 400ml or 200ml for use according to blood sampling volume, required blood volume is taked in aseptic technique, from then disinfection according to circumstances of body storage blood person; Blood sampling finishes to reserve the blood that cross matching is used after the back seals heparin tube segmentation heat seal, cuts off unnecessary part, can do cross matching when making Washed Red Blood Cells and use; If the making frozen red cells can only be reserved the heparin tube of one section sky in advance, wherein the Red Blood Cells Frozen and Deglycerolized perfusion for preparing, segmentation heat seal sealing, the catheter perfusion blood plasma of plasma bags, segmentation heat seal sealing, with frozen red cells mark stored frozen, cross matching is used.At first the syringe needle of plasma bags and waste fluid bag liquid-inlet pipe, switch, conduit, case lid 10 from negative-pressure sealing case 9 passes respectively, outside the outlet box, build loam cake 10 and make it sealing, blood taking bag 5 strainers 6 are hung on the hook of operation cabinet down, and with the combination liquid fillers link to each other, according to the requirement of aseptic technique with 75% alcohol disinfecting after, the 4-5 syringe needle of combination liquid fillers is thrust among the liquid-inlet pipe 7-1 of blood taking bag 5 from the head, the liquid-inlet pipe 8-1 of plasma bags and waste fluid bag, two syringe needles of 8-2 thrust two liquid discharge pipe 7-3 of blood taking bag respectively from the head, among the 7-4, the extraction pipe 12 of negative-pressure sealing case 9 is connected with vacuum pump 13, open the power supply of vacuum pump 13, begin to bleed, turn on the switch of plasma bags leakage fluid dram, suction filtration blood plasma, suction filtration is closed after finishing, and this bag blood plasma can be used to make refrigerated plasma usefulness, makes full use of blood resource, the catheter perfusion blood plasma of plasma bags, segmentation heat seal sealing, with frozen red cells mark stored frozen, cross matching is used; Turn on the liquid adding bottle switch of the physiological saline of combination liquid feeding bag, in blood taking bag, add physiological saline, close after filling it up with, blood taking bag put upside down gently be mixed, turn on the switch of waste fluid bag, suction filtration is screwed on after finishing, this process can be carried out repeatedly till cleaning, leucocyte filter, filtering removing leukocytes can be connected when in particular cases needing to remove white corpuscle, also can assist according to handling, the deactivation white corpuscle when preparing photosensitive sterilization, adds its switch of screwing on behind the photosensitizers, illumination carries out disinfection, and sterilization finishes the back photosensitizers suction filtration is gone in the waste fluid bag; Turn on the normal saline bag switch and repeat washing process, till cleaning, can add 100ml or 200ml physiological saline during the preparing washing red corpuscle or not contain the rbc preservative of antithrombotics; When preparing stored frozen, turn on the switch of the glycerine preservation liquid bag in the composite reagent bag, slowly added glycerine and preserve liquid in 30 minutes, vibration is mixed, and equilibrium at room temperature is after 30 minutes, pull up two syringe needles of combination liquid fillers 4-5 and liquid-inlet pipe 8-1,8-2, cap on the sterilization bonnet is closed the power supply of vacuum pump 13, can be in blood taking bag and filter middle portion heat seal sealing, prevent to pollute, hemocyte and in have the plasma bags conduit of the heat seal sealing of blood plasma directly to put-80 ℃ of freezing preservations of refrigerator-freezer very low temperature.
The erythrocytic setting drawing of preparation thawing to reduce glycerol as shown in Figure 2, it and the erythrocytic difference of preparing washing only are the waste fluid bag that is of the interior placement of negative-pressure sealing case, and the liquid discharge pipe 7-2 of blood taking bag 5 is connected with the liquid-inlet pipe 8 of waste fluid bag.At first, when melting red corpuscle earlier add and use reagent, vacuum pump and negative-pressure sealing case are checked the errorless corresponding position that is placed on, the extraction pipe 12 of negative-pressure sealing case 9 is connected with vacuum pump 13, connect power supply, syringe needle, switch, the conduit of the waste fluid bag in the negative-pressure sealing case 9 are passed from the loam cake 10 of negative-pressure sealing case 9, build loam cake 10 then, make its sealing, add, be hung in combination of agents reagent bag on the composite reagent bag hook 2 of operation cabinet 1, syringe needle, switch, catheter segment below the opening of liquid fillers base plate is drawn out to liquid fillers; The blood taking bag that frozen red cells is housed is put into 37 ℃ of constant water bath box middling speeds to be melted, in 10 minutes, melt, rapidly blood taking bag 5 is hung on the hook of operation cabinet, the below of combination liquid fillers, sterilization back aseptic technique, with waste fluid bag 14-1,14-2,14-3, the syringe needle of the waste fluid bag liquid-inlet pipe 8 that 14-4 links to each other links to each other with the leakage fluid dram 7-2 of blood taking bag 5,4-5 links to each other with blood taking bag liquid-inlet pipe 7-1 combination liquid feeding organ pipe, open vacuum pump, turn on the switch of waste fluid bag, suction filtration goes out glycerine and preserves liquid, the switch of screwing on, repeat above-mentioned washing step, wash the 1st time with 9% sodium chloride solution and 6% hydroxyethyl starch solution, wash the 2nd time with 6% hydroxyethyl starch solution and 0.9% sodium chloride solution again, after washing is finished, blood sampling volume is the perfusion 100ml physiological saline of 200ml, and blood sampling volume is the perfusion 200ml physiological saline of 400ml; Melt the red cell suspension of at once transfusing blood the back and can after washing, directly pour into the aseptic apyrogenic physiological saline that contains 1% hydrogen peroxide of equivalent, also can preserve red corpuscle, make the thawing to reduce glycerol red corpuscle with other several alserver's solutions that do not contain antithrombotics.
Claims (4)
1, the method for a kind of preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized is characterized in that comprising the steps:
(1) preparing washing red corpuscle and frozen red cells
(1) use the blood taking bag (5) that has strainer (6) under aseptic situation, to gather blood;
(2) with blood taking bag (5) with have combination liquid fillers (4-1), (4-2), (4-3), the composite reagent bag (3-1) of (4-4), (3-2), (3-3), (3-4) is connected, the composite reagent bag is positioned at the top of blood taking bag (5);
(3) plasma bags and waste fluid bag (14-1), (14-2), (14-3), (14-4) are put into negative-pressure sealing case (9), close negative-pressure sealing case (9), plasma bags is connected with the liquid discharge pipe of blood taking bag with waste fluid bag, negative-pressure sealing case (9) is placed on the below of blood taking bag (5), and is connected with vacuum pump;
(4) open vacuum pump, make in the negative-pressure sealing case (9) to be negative pressure state;
(5) be under the negative pressure state in plasma bags and waste fluid bag (14-1), (14-2), (14-3), (14-4), in blood taking bag (5), add corresponding reagent successively, blood is carried out suction filtration, washing and sterilization, emit blood plasma and waste liquid, make Washed Red Blood Cells; Or in blood taking bag, add glycerine preservation liquid, in order to preserving;
(6) take off blood taking bag (5), it is preserved at-80 ℃ of very low temperature, get frozen red cells;
(2) preparation thawing to reduce glycerol red corpuscle
(1) takes out the blood taking bag (5) that glycerine preservation liquid is housed, put into 37 ℃ of constant water bath box, make its thawing;
(2) connect composite reagent bag (3-1), (3-2), (3-3), (3-4) and the negative-pressure sealing case (9) that has combination liquid fillers (4-1), (4-2), (4-3), (4-4), 9% sodium chloride solution, 6% hydroxyethyl starch solution, 0.9% sodium chloride solution are housed respectively among composite reagent bag (3-1), (3-2), (3-3), (3-4) and contain the aseptic apyrogenic physiological saline of 1% hydrogen peroxide, waste fluid bag is put into negative-pressure sealing case (9), close negative-pressure sealing case (9), the extraction pipe of negative-pressure sealing case (12) links to each other with vacuum pump (13);
(3), make leakage fluid dram (7-1), (7-2), (7-3), (7-4) of blood taking bag and be placed on the interior waste fluid bag of negative-pressure sealing case to be connected with composite reagent bag (3-1), (3-2), (3-3), (3-4) and glycerine is housed preserves the blood taking bag fluid inlet (7-1) of liquid and link to each other;
(4) open vacuum pump; make the waste fluid bag in the negative-pressure sealing case (9) be in negative pressure state; elder generation's suction filtration goes out glycerine protection liquid; wash the 1st time with 9% sodium chloride solution and 6% hydroxyethyl starch solution; wash the 2nd time with 6% hydroxyethyl starch solution and 0.9% sodium chloride solution again; after washing is finished, directly pour into the aseptic apyrogenic physiological saline that contains 1% hydrogen peroxide of equivalent, make the thawing to reduce glycerol red corpuscle.
2, a kind of device that is used for described preparing washing red corpuscle of claim 1 and Red Blood Cells Frozen and Deglycerolized method, it is characterized in that comprising in the structure blood taking bag (5), the bottom of blood taking bag (5) has strainer (6), in the top of blood taking bag (5) is composite reagent bag (3-1), 3-2,3-3,3-4), the below of blood taking bag (5) is negative-pressure sealing case (9), interior dress plasma bags and waste fluid bag (14-1,14-2,14-3,14-4), the extraction pipe (12) of negative-pressure sealing case (9) links to each other with vacuum pump (13), plasma bags and waste fluid bag (14-1 in the negative-pressure sealing case (9), 14-2,14-3,14-4) be in negative pressure state.
3, by the described device that is used for preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized method of claim 2, it is characterized in that: device has cabinet type structure, the composite reagent bag hangs on the superiors' hooks (2) of operation cabinet (1), blood taking bag (5) is carried out liquid feeding by action of gravity, negative-pressure sealing case (9) is positioned at the below of operation cabinet.
4, by claim 2 or the 3 described devices that are used for preparing washing red corpuscle and Red Blood Cells Frozen and Deglycerolized method, it is characterized in that: said strainer is a membrane filter, filter core and strainer by the filter membrane that has micropore, sealing-ring, band sieve aperture are configured to outward, the filter membrane of band micropore is placed on above the filter core of band sieve aperture, seal by sealing-ring, add a cover the strainer outrigger above, be fixed on the bottom of blood taking bag (5), fastening the linking to each other of leakage fluid dram of filter core and blood taking bag by support.
Priority Applications (1)
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CN 200410012306 CN1266270C (en) | 2004-05-24 | 2004-05-24 | Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte |
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CN 200410012306 CN1266270C (en) | 2004-05-24 | 2004-05-24 | Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte |
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CN1590540A CN1590540A (en) | 2005-03-09 |
CN1266270C true CN1266270C (en) | 2006-07-26 |
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CN 200410012306 Expired - Fee Related CN1266270C (en) | 2004-05-24 | 2004-05-24 | Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte |
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1325518C (en) * | 2005-09-09 | 2007-07-11 | 李勇 | Cell membrane antigen preservation method and magnetic particle envelope human cell membrane antigen |
CN101333514B (en) * | 2007-06-29 | 2010-10-27 | 上海市血液中心 | Rapid freezing and thawing process for erythrocyte in refrigerator and freezing protection liquid and scrubbing liquid used by the process |
CN101904331B (en) * | 2010-07-14 | 2014-04-16 | 樊福好 | Blood sample protecting solution and method for collecting blood sample |
CN103861161B (en) * | 2012-12-18 | 2016-06-08 | 苏州排头兵药业科技有限公司 | The preparation method of Washed Red Blood Cells device and Washed Red Blood Cells |
CN104789526B (en) * | 2015-05-05 | 2018-02-16 | 宋毅 | A kind of method and apparatus for preparing Red Blood Cells Frozen and Deglycerolized |
CN105505760B (en) * | 2015-12-31 | 2018-05-25 | 上海白泽医疗器械有限公司 | The full-automatic non-closed cell washing system of centrifugation |
CN110331081B (en) * | 2019-06-06 | 2022-09-23 | 武汉仝干医疗科技股份有限公司 | Cell washing container, cell collecting and washing method and system |
CN110496259B (en) * | 2019-08-29 | 2021-09-07 | 成都行泰生物科技有限公司 | Red blood cell collecting and recycling device |
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