CN103861161B - RBC washing apparatus and method for preparing the washed erythrocytes - Google Patents

RBC washing apparatus and method for preparing the washed erythrocytes Download PDF

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CN103861161B
CN103861161B CN 201210550175 CN201210550175A CN103861161B CN 103861161 B CN103861161 B CN 103861161B CN 201210550175 CN201210550175 CN 201210550175 CN 201210550175 A CN201210550175 A CN 201210550175A CN 103861161 B CN103861161 B CN 103861161B
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bag
plasma
blood
red blood
flow
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CN103861161A (en )
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韩兆法
陈光增
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苏州排头兵药业科技有限公司
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Abstract

<b>一种洗涤红细胞装置及洗涤红细胞的制备方法,包括瓶塞穿刺器、血液过滤网、入管口通过导流管与血液过滤网的出管口相连接的洗涤袋、血浆储存袋、废液收集袋、入管口与洗涤袋的入管口相连接的红细胞储存袋,洗涤袋的出管口、废液收集袋的入管口、血浆储存袋的入管口三者相连接,洗涤袋包括血袋、位于血袋内腔中膜袋,血袋的入口边部与膜袋的入口边部相连接,血浆储存袋的入管设置有血浆止流夹,废液收集袋的入管设置有废液止流夹,红细胞储存袋的入管设置有红细胞止流夹,导流管上设置有导流管止流夹。 <B> a washing apparatus and a method for preparing erythrocyte washed red blood cells, comprising Spikes, blood filter, wash the bag spout into the draft tube through the blood filter of the nozzle connected to the plasma storage bag, the waste the nozzle three liquid collection bag, red blood cell storage bag into the spout the washing bag into the spout is connected, the washing bag out spout, the waste collection bag into the spout, the plasma storage bag is connected to the washing bag includes a blood bag located in the lumen of the blood bag film bag, the inlet side of the inlet side blood bag and the membrane portion is connected to the bag, the storage bag into the plasma with a plasma flow stop pipe clip is provided, the waste pipe is provided with a waste collection bag only flow folder, into a red blood cell storage bag provided with a tube stop the flow of red blood cells interposed, provided only flow diversion pipe clip draft tube. 本发明中主要利用血浆或洗涤液依其静压能够自然透过膜袋,而红细胞不能透过,使红细胞与血浆或洗涤液能够分离,分离后的红细胞较完好。 The present invention mainly use plasma or wash liquor in accordance with its hydrostatic pressure pockets can be naturally permeable membrane, impermeable erythrocytes, or red blood cells and plasma can be separated from the washing liquid, after the separation of red blood cells compared to intact. </b> </ B>

Description

洗涤红细胞装置及洗涤红细胞的制备方法 RBC washing apparatus and method for preparing the washed erythrocytes

技术领域 FIELD

[0001 ]本发明涉及一种洗涤红细胞装置及洗涤红细胞的制备方法。 [0001] The present invention relates to a device and a method for washing erythrocytes were washed erythrocytes.

背景技术 Background technique

[0002]目前GB18469《全血及成分血质量要求》中规定的洗涤红细胞的制备,均依据中华人民共和国卫生部颁发《血站技术操作规程》中的离心法制备,即将全血或红细胞悬液经专用离心机离心,手工操作分离出血浆后,反复采用离心法用洗涤液将红细胞洗涤。 Preparation of washed red blood cells of a predetermined [0002] It GB18469 "whole blood and blood components quality" in both issued prepared by centrifugal casting "Blood technical rules" in the Ministry of Health of People's Republic of China based, i.e. whole blood or red blood cells via dedicated centrifuge, plasma was separated after the manual operation is repeated with a washing solution using centrifugation, washed red blood cells. 如中国专利CN201073450公布了一种一次性使用红细胞洗涤装置,其包括离心洗涤杯、细菌过滤器、废液收集袋、洗涤红细胞收集袋等。 Chinese patent CN201073450 discloses a disposable RBC washing apparatus comprising washing centrifuge cup, bacteria filter, the waste collection bag, washed red blood collection bag and the like. 利用离心功能实现红细胞与血浆分离时,红细胞损害较大。 When the functions implemented by centrifugal separation of red blood cells and plasma, red blood cells greater damage.

发明内容 SUMMARY

[0003]本发明要解决的第一个技术问题是提供一种洗涤红细胞装置。 [0003] The first technical problem to be solved by the present invention is to provide an apparatus washed red blood cells.

[0004]为了解决上述技术问题,本发明采用的一种技术方案是:一种洗涤红细胞装置,包括瓶塞穿刺器、入管口与所述的瓶塞穿刺器相连接的血液过滤网、入管口通过导流管与所述的血液过滤网的出管口相连接的洗涤袋、血浆储存袋、废液收集袋、入管口与所述的洗涤袋的入管口相连接的红细胞储存袋,所述的洗涤袋的出管口、所述的废液收集袋的入管口、所述的血浆储存袋的入管口三者相连接,所述的洗涤袋包括血袋、位于所述的血袋内腔中的能够透过水溶液但不能透过红细胞的膜袋,所述的血袋的入口边部与所述的膜袋的入口边部相连接,所述的血浆储存袋的入管设置有血浆止流夹,所述的废液收集袋的入管设置有废液止流夹,所述的红细胞储存袋的入管设置有红细胞止流夹,所述的导流管上设置有导流管止流夹。 [0004] In order to solve the above problems, the present invention adopts a technical solution is: A means washed red blood cells, comprising Spikes, the blood inlet tube and said Spikes filter connected, the spout washing the bag through the draft tube and the blood filter is connected to the nozzle erythrocyte plasma storage bag storage bag, the waste collection bag, the wash bag spout into the nozzle connected to the spout into the bag and washed three out spout, said waste liquid collecting bag spout into said plasma storage bag is connected to the washing bag comprises a blood bag, blood bag in said lumen the red blood cells can, but impermeable film bag, the inlet side of the inlet side portion of the blood bag and the bag is connected to a film through an aqueous solution of the plasma storage bag into the plasma tube is provided with only flow clip, the waste collection bag into the pipe clip is provided with a waste flow stop, the red blood cell storage bag into the red blood cell tube is provided with a retaining clip stream provided with a draft tube flow stop clamps the draft tube.

[0005]在某些实施方式中,所述的废液收集袋装有空气过滤器。 [0005] In certain embodiments, the waste collection bag contains an air filter.

[0006]在某些实施方式中,所述的血袋的左右两侧边部与所述的膜袋的左右两侧边部相连接。 [0006] In certain embodiments, the left and right side edge portions of the left and right both side portions of the blood bag and the bag is connected to a film.

[0007]在某些实施方式中,所述的瓶塞穿刺器、所述的血液过滤网、所述的导流管、所述的洗涤袋、所述的血浆储存袋沿着从上到下的顺序分布设置。 [0007] In certain embodiments, the Spikes, a blood filter, said draft tube, washing the bag along the plasma reservoir bag from top to bottom sequence distribution settings.

[0008]本发明要解决的第二个技术问题是提供一种洗涤红细胞装置。 [0008] The present invention is to solve the second technical problem is to provide an apparatus washed red blood cells.

[0009]为了解决上述技术问题,本发明采用的一种技术方案是:一种洗涤红细胞装置,包括瓶塞穿刺器、入管口与所述的瓶塞穿刺器相连接的血液过滤网、入管口通过导流管与所述的血液过滤网的出管口相连接的洗涤袋、白细胞过滤器、血浆储存袋、废液收集袋、入管口与所述的洗涤袋的入管相连接的红细胞储存袋,所述的白细胞过滤器的入管口、所述的导流管的入管口与所述的血液过滤网的出管口相连接,所述的导流管的出管口、所述的白细胞过滤器的出管口与所述的洗涤袋的入管口相连接,所述的白细胞过滤器与所述的导流管相并列的设置,所述的洗涤袋的出管口、所述的废液收集袋的入管口、所述的血浆储存袋的入管口三者相连接,所述的洗涤袋包括血袋、位于所述的血袋内腔中的能够透过水溶液但不能透过红细胞的膜袋,所述的血袋的入口边 [0009] In order to solve the above problems, the present invention adopts a technical solution is: A means washed red blood cells, comprising Spikes, the blood inlet tube and said Spikes filter connected, the spout bags washed RBC storage bag through the draft tube and the blood filter is connected to the nozzle, leucocyte filter, the plasma storage bag, the waste collection bag, the spout of the bag into the washing tube connected , the leukocyte filter into the nozzle, the nozzle of the draft tube with the blood of the filter is connected to the nozzle, the nozzle according to the draft tube, said leukocyte filter washing out of the bag with said spout is connected into the inlet tube, the leukocyte filter and the draft tube is disposed in parallel with the washing out of the bag spout, the waste collecting bag into the spout, the spout of the three plasma storage bag is connected to a washing bag comprises a blood bag, a blood bag in said lumen of erythrocytes can be permeable, impermeable film solution an inlet side blood bag bags, according to 部与所述的膜袋的入口边部相连接,所述的血浆储存袋的入管设置有血浆止流夹,所述的废液收集袋的入管设置有废液止流夹,所述的红细胞储存袋的入管设置有红细胞止流夹,所述的导流管上设置有导流管止流夹,所述的白细胞过滤器的入管上设置有白细胞过滤前止流夹,所述的白细胞过滤器的出管上设置有白细胞过滤后止流夹。 An inlet edge portion of the film bag is connected into the plasma tube is provided with a plasma storage bag retaining clip stream, into the waste tube is provided with a waste collecting bag retaining clip stream, the red blood cells the tube is provided with a red blood cell storage bag retaining clip stream, provided with a draft tube flow stop clamps the guide tube, is provided on the tube into the leukocyte filter leukocytes before filtration flow stop clip, said leukocyte filter after the clip is provided with a flow stop on the leukocyte depletion filter tube.

[0010]在某些实施方式中,所述的废液收集袋装有空气过滤器。 [0010] In certain embodiments, the waste collection bag contains an air filter.

[0011]在某些实施方式中,所述的血袋的左右两侧边部与所述的膜袋的左右两侧边部相连接。 [0011] In certain embodiments, the left and right side edge portions of the left and right both side portions of the blood bag and the bag is connected to a film.

[0012]在某些实施方式中,所述的瓶塞穿刺器、所述的血液过滤网、所述的白细胞过滤器、所述的洗涤袋、所述的血浆储存袋沿着从上到下的顺序分布设置。 [0012] In certain embodiments, the Spikes, a blood filter, said leukocyte filter, wash the bag along the plasma reservoir bag from top to bottom sequence distribution settings.

[0013]本发明要解决的第三个技术问题是提供一种洗涤红细胞的制备方法。 [0013] The present invention is to solve the third technical problem is to provide a washed erythrocyte preparation.

[0014]为了解决上述技术问题,本发明采用的一种技术方案是:一种采用第一个技术问题中的所述的洗涤红细胞装置实现的洗涤红细胞制备方法,包括以下步骤: [0014] In order to solve the above problems, the present invention adopts a technical solution is: A method for the preparation of washed red blood cells first technical problem in the washed red blood cells implemented using apparatus, comprising the steps of:

[0015] —、将所述的瓶塞穿刺器、所述的血液过滤网、所述的导流管、所述的洗涤袋、所述的血浆储存袋沿着从上到下的顺序分布设置,将所述的导流管止流夹与所述的血浆止流夹置于打开位置,将所述的红细胞止流夹与所述的废液止流夹都置于关闭位置; [0015] - the said Spikes, a blood filter, said draft tube, the washing bag the plasma storage bag is provided along a distribution order from the top , the draft tube clamp of the flow stop plasma flow stopping clip in the open position, the flow of red blood cells retaining clip and stop the waste stream are interposed in the closed position;

[0016] 二、将所述的瓶塞穿刺器插入已去除白细胞的血液袋的输血插口中,血液流经所述的血液过滤网过滤,而后通过所述的导流管流入所述的洗涤袋,血液中的红细胞截留在所述的膜袋内,血液中的血浆透过所述的膜袋流入所述的血浆储存袋,当血浆完全流入所述的血浆储存袋后,将所述的血浆止流夹置于关闭位置; [0016] Second, the cork has been removed is inserted into the piercing bags of blood leukocytes socket transfusion, the blood flowing through the blood filtration filter, and then washed according to the bag flows through said flow tube , red blood cells trapped in the film bag, the blood plasma through the membrane pocket into said plasma storage bag, when the plasma completely into said plasma storage bag, the plasma flow stop is interposed in the closed position;

[0017]三、将所述的瓶塞穿刺器插入洗涤液容器中,洗涤液流入所述的洗涤袋的膜袋内,并且边加洗涤液边摇动所述的血袋,使得洗涤液与所述的膜袋内的红细胞充分混合,将所述的废液止流夹置于打开位置,使得所述的膜袋内的洗涤废液完全流入所述的废液收集袋,将所述的废液止流夹置于关闭位置,如此形成一个清洗步骤,该步骤连续操作1-6次后,清洗完毕; [0017] Third, the insertion of Spikes washing liquid container, washing the membrane pockets wash liquid into said pocket, and the cleaning liquid while adding bags shaking the edge, so that the washing solution and erythrocytes mixed bag film described later, to stop the waste stream clip in the open position, so that the washing waste liquid film pouch completely into the waste collection bag according to, the waste after the liquid flow stop interposed in the closed position, thus forming a cleaning step, this step 1-6 times continuous operation, cleaning is completed;

[0018]四、将所述的瓶塞穿刺器插入洗涤液容器中,洗涤液流入所述的洗涤袋的膜袋内,并且边加洗涤液边摇动所述的血袋,使得洗涤液与所述的膜袋内的红细胞充分混合,关闭所述的导流管止流夹,打开所述的红细胞止流夹,拎起所述的洗涤袋,使得所述的膜袋内的红细胞流入所述的红细胞储存袋,打开所述的导流管止流夹,补加洗涤液直至所述的红细胞储存袋内的血液容积达到标准规定,关闭所述的红细胞止流夹,洗涤红细胞制备完成。 [0018] Fourth, the the washing liquid is inserted Spikes vessel, washing the membrane pockets wash liquid flowing into the pouch, and while adding bags shaking the washing liquid side, so that the washing solution and erythrocytes sufficiently mixed film of said bag, closing said flow conduit flow stop clip, open the clip stream stop erythrocytes, washing the bags picked up, so that the red blood cell membrane into said pouch RBC storage bag, opening the draft tube retaining clip stream, supplemented with RBC storage bag until the wash liquor reaches the standard blood volume, closing the erythrocytes retaining clip stream, to complete the preparation of washed red blood cells.

[0019]本发明要解决的第四个技术问题是提供一种洗涤红细胞的制备方法。 [0019] The present invention is to solve the fourth technical problem is to provide a washed erythrocyte preparation.

[0020]为了解决上述技术问题,本发明采用的一种技术方案是:一、所述的瓶塞穿刺器、所述的血液过滤网、所述的白细胞过滤器、所述的洗涤袋、所述的血浆储存袋沿着从上到下的顺序分布设置,将所述的白细胞过滤前止流夹、所述的白细胞过滤后止流夹与所述的血浆止流夹置于打开位置,将所述的导流管止流夹、所述的红细胞止流夹与所述的废液止流夹都置于关闭位置; [0020] To solve the above problem, one aspect of the present invention is that: a said Spikes, a blood filter, said leukocyte filter, said washing bag is said plasma storage bag along the distribution order from setting prior to the leukocyte filter retaining clip stream after the flow of the leukocyte filter retaining clip stop of the plasma stream interposed open position, stopping flow of the draft tube clamp, the erythrocyte flow stopping clip and stop the waste stream are interposed in the closed position;

[0021] 二、将所述的瓶塞穿刺器插入待洗涤血液袋的输血插口中,血液流经所述的血液过滤网过滤,而后流入所述的白细胞过滤器,血液中的白细胞截留在所述的白细胞过滤器内,然后去除白细胞的血液流入所述的洗涤袋,血液中的红细胞截留在所述的膜袋内,血液中的血浆透过所述的膜袋流入所述的血浆储存袋,当血浆完全流入所述的血浆储存袋后,将所述的白细胞过滤前止流夹、所述的白细胞过滤后止流夹与所述的血浆止流夹置于关闭位置,将所述的导流管止流夹置于打开位置; [0021] Second, the blood outlet of the insert to be washed Spikes bags of blood, the blood flowing through the blood filtration filter, and then flows into the leucocyte filter, the white blood trapped in the leukocyte filter described later, the bag is then removed and washed leukocytes in the blood flowing into the blood erythrocytes entrapped in said film bag, the blood plasma flows through the plasma storage bag membrane bag , when the plasma completely into said plasma storage bag, the flow stop before filtering leukocytes clip, after stopping flow of the leukocyte filter and the plasma retaining clip stream interposed closed position, the only flow diversion pipe clamp in the open position;

[0022] 三、将所述的瓶塞穿刺器插入洗涤液容器中,洗涤液流经所述的血液过滤网、所述的导流管,流入所述的洗涤袋的膜袋内,并且边加洗涤液边摇动所述的血袋,使得洗涤液与所述的膜袋内的红细胞充分混合,将所述的废液止流夹置于打开位置,使得所述的膜袋内的洗涤废液完全流入所述的废液收集袋,将所述的废液止流夹置于关闭位置,如此形成一个清洗步骤,该步骤连续操作1-6次后,清洗完毕; [0022] Third, the insertion of Spikes washing liquid vessel, the blood flow through the filter is washed, said draft tube, the membrane pockets flows into the washing bag and side shaking the washing liquid plus side of the blood bag, the bag so that the red blood cell membranes mixed with the washing liquid, the waste stream retaining clip in the open position, so that the washing waste film pouch after flowing into the waste liquid completely collecting bag, stopping the flow of waste closed position interposed, thus forming a cleaning step, this step 1-6 times continuous operation, cleaning is completed;

[0023]四、将所述的瓶塞穿刺器插入洗涤液容器中,洗涤液流入所述的洗涤袋的膜袋内,并且边加洗涤液边摇动所述的血袋,使得洗涤液与所述的膜袋内的红细胞充分混合,关闭所述的导流管止流夹,打开所述的红细胞止流夹,拎起所述的洗涤袋,使得所述的膜袋内的红细胞流入所述的红细胞储存袋,打开所述的导流管止流夹,补加洗涤液,直至所述的红细胞储存袋内的血液容积达到标准规定,关闭所述的红细胞止流夹,洗涤红细胞制备完成。 [0023] Fourth, the the washing liquid is inserted Spikes vessel, washing the membrane pockets wash liquid flowing into the pouch, and while adding bags shaking the washing liquid side, so that the washing solution and erythrocytes sufficiently mixed film of said bag, closing said flow conduit flow stop clip, open the clip stream stop erythrocytes, washing the bags picked up, so that the red blood cell membrane into said pouch RBC storage bag, opening the draft tube retaining clip stream, additional wash liquid, until the red blood cell storage bag of blood volume in a standard, off the flow of red blood cells retaining clip, to complete the preparation of washed red blood cells.

[0024]本发明的范围,并不限于上述技术特征的特定组合而成的技术方案,同时也应涵盖由上述技术特征或其等同特征进行任意组合而形成的其它技术方案。 [0024] scope of the present invention is not limited to the above specific combination of technical features of the technical solution should also be covered by the other aspect of the technical features or any combination equivalents are formed. 例如上述特征与本申请中公开的(但不限于)具有类似功能的技术特征进行互相替换而形成的技术方案等。 The above features and, for example, disclosed in the present application (without limitation) having technical features similar function will be formed by the replacement of another aspect of the like.

[0025]由于上述技术方案运用,本发明与现有技术相比具有下列优点:本发明中主要利用血浆或洗涤液依其静压能够自然透过膜袋,而红细胞不能透过,致使红细胞与血浆或洗涤液能够分离,分离后的红细胞较完好,符合GB18469要求。 [0025] Using the above technical solution, the present invention as compared with the prior art has the following advantages: the present invention mainly use plasma or wash liquor in accordance with its hydrostatic pressure pockets can be naturally permeable membrane, impermeable erythrocytes, resulting in red blood cells or washing liquid can be separated from plasma, red blood cells separated relatively intact, GB18469 meet the requirements.

附图说明 BRIEF DESCRIPTION

[0026]附图1为本发明不意图(实施例一); [0026] Figure 1 is not intended to (first embodiment) of the present invention;

[0027]附图2为本发明示意图(实施例二); [0027] FIG 2 is a schematic view of the invention (second embodiment);

[0028] 其中:1、血液过滤网;2、导流管;3、白细胞过滤器;4、洗涤袋;41、膜袋;42、血袋;5、血浆储存袋;6、废液收集袋;7、红细胞储存袋;8、瓶塞穿刺器;11、导流管止流夹;12、白细胞过滤前止流夹;13、白细胞过滤后止流夹;14、血浆止流夹;15、废液止流夹;16、红细胞止流夹。 [0028] wherein: a blood filter; 2, guide tubes; 3, leucocyte filter; 4, wash bags; 41, the membrane bags; 42, blood bags; 5, the plasma reservoir bag; 6, the waste collection bag ; 7, RBC storage bag; 8, Spikes; 11, only flow diversion pipe clamp; 12, the front clip leukocyte filtration flow stop; 13, leukocyte filtration flow stop clip; 14, stopping the flow of plasma folder; 15, waste stream clip stop; 16, red blood cell flow stop clip.

具体实施方式 detailed description

[0029]实施例一:如I附图所示,一种洗涤红细胞装置,包括瓶塞穿刺器8、入管口与瓶塞穿刺器8相连接的血液过滤网1、入管口通过导流管2与血液过滤网I的出管口相连接的洗涤袋4、白细胞过滤器3、血浆储存袋5、废液收集袋6、入管口与洗涤袋4的入管口相连接的红细胞储存袋7。 [0029] Example a: I as shown in the accompanying drawings, a washing erythrocytes apparatus comprising Spikes 8, the nozzle 8 with Spikes blood filter 1 is connected, through a nozzle into a draft tube 2 the blood filter washing the bag I of a nozzle 4 connected, leucocyte filter 3, the plasma storage bag 5, the waste collection bag 6, erythrocyte storage bag into the spout of the washing - bag spout 4 is connected to 7.

[0030]白细胞过滤器3的入管口、导流管2的入管口与血液过滤网I的出管口相连接,导流管2的出管口、白细胞过滤器3的出管口与洗涤袋4的入管口相连接,白细胞过滤器3与导流管2相并列的设置,洗涤袋4的出管口、废液收集袋6的入管口、血浆储存袋5的入管口三者相连接。 The nozzle and the blood [0030] leukocyte filter into the spout 3, the draft tube 2 of the filter I of the nozzle is connected to the draft tube out spout 2, the leukocyte filter out spout the washing bag 3 into the spout 4 is connected, leucocyte filter 3 and the draft tube 2 with parallel disposed, washed bags the spout 4, the waste collection bag into the spout 6, the plasma storage bag into the spout of the three 5 is connected.

[0031] 洗涤袋4包括血袋42、位于血袋42内腔中的能够透过血浆但不能透过红细胞的膜袋41,血袋42的入口边部与膜袋41的入口边部相连接,如粘接关于膜袋41的材料一般为高分子材料,应具有透过血浆,阻留红细胞、亲水、无毒、具血液相容性和一定的机械强度等特性。 [0031] The bag 4 comprises a blood bag 42 is washed, the blood bag 42 is located in lumen 41 but not capable of plasma, the inlet side of the inlet portion of the bag film side portion 42 of the bags 41 is connected through a permeable membrane pockets erythrocytes as the adhesive material on the bag film 41 is typically a polymer material, should have through plasma, erythrocytes retention characteristics, the hydrophilic, non-toxic, compatible with blood and certain mechanical strength. 只要是密度能够允许血浆透过,而红细胞不能透的材料都可适用,如可以无纺布、复合纤维膜等。 As long as the plasma density can allow permeation red blood cells can not penetrate the material may be suitable, as may be non-woven, fibrous composite film.

[0032]血浆储存袋5的入管设置有血浆止流夹14,废液收集袋6的入管设置有废液止流夹15,红细胞储存袋7的入管设置有红细胞止流夹16,导流管2上设置有导流管止流夹11,白细胞过滤器3的入管上设置有白细胞过滤前止流夹12,白细胞过滤器3的出管上设置有白细胞过滤后止流夹13。 [0032] The plasma storage bag into the pipe 5 is provided with a plasma flow stop clip 14, the waste collection bag into the pipe 6 is provided with a waste flow stop clip 15, red blood cell storage bag into the pipe 7 is provided with a red blood cell flow stop clip 16, draft tube 2 is provided with a draft tube flow stop clip 11, is provided with a white blood leukocyte filter before filtration flow stop clip 12, leucocyte filter 13 is provided with a retaining clip stream after the leukocyte-removing filter tube 3 into the tube 3.

[0033]瓶塞穿刺器8、血液过滤网1、白细胞过滤器3、止流夹、废液收集袋6等属于现有技术,具体结构在此不再赘述。 [0033] Spikes 8, the blood filter 1, leucocyte filter 3, the flow stop clip, the waste liquid collecting bag 6 and the like belong to the prior art, specific structural omitted.

[0034]瓶塞穿刺器8上有保护套,瓶塞穿刺器8用于插入装有血液或洗涤液的容器中。 [0034] a protective sleeve 8 Spikes, Spikes 8 for inserting a container containing blood or wash liquor in.

[0035]血浆储存袋与红细胞储存袋袋体上印制容量刻度线,便于操作者控制相应内容物的容量。 [0035] The plasma and red blood cell storage bag storage capacity of the mark printed on the bag body, for the operator to control the capacity of the corresponding contents. 废液储存袋一侧装有空气过滤器。 A waste liquid storage bag with one side of the air filter. 所述的血袋的左右两侧边部与所述的膜袋的左右两侧边部也相连接,如粘接。 Left and right side edge portions of the left and right both side portions of the blood bag and the bag film are also connected, such as an adhesive.

[0036] 瓶塞穿刺器8、血液过滤网1、白细胞过滤器3、洗涤袋4、血浆储存袋5沿着从上到下的顺序分布设置。 [0036] Spikes 8, the blood filter 1, 3 leucocyte filter, washed bag 4, the distribution of the plasma storage bag 5 is provided along the top to bottom.

[0037] —种采用上述洗涤红细胞装置实现的洗涤红细胞制备方法,包括以下步骤: [0037] - seed preparation method of the washed red blood cells RBC washing apparatus implemented, comprising the steps of:

[0038] —、瓶塞穿刺器8、血液过滤网1、白细胞过滤器3、洗涤袋4、血浆储存袋5沿着从上到下的顺序分布设置,将白细胞过滤前止流夹12、白细胞过滤后止流夹13与血浆止流夹14置于打开位置,将导流管止流夹11、红细胞止流夹16与废液止流夹15都置于关闭位置; [0038] -, Spikes 8, the blood filter 1, 3 leucocyte filter, washed with bags 4, 5 in the plasma storage bag set distribution order from the top, the white blood cell filtration flow stop before the clip 12, WBC after filtration only flow 13 and plasma flow stop clip clamp 14 in the open position, stopping the flow of the draft tube clip 11, clip 16 and the red blood cells flow stop waste flow stop clip 15 are in the closed position;

[0039] 二、将瓶塞穿刺器8插入待洗涤血液袋的输血插口中,血液流经血液过滤网I过滤,而后流入白细胞过滤器3,血液中的白细胞截留在白细胞过滤器3内,然后去除白细胞的血液流入洗涤袋4,血液中的红细胞截留在膜袋41内,血液中的血浆透过膜袋41流入血浆储存袋5,当血浆完全流入血浆储存袋5后,将白细胞过滤前止流夹12、白细胞过滤后止流夹13与血浆止流夹14置于关闭位置,将导流管止流夹11置于打开位置; Transfusion Socket [0039] Second, the Spikes 8 is inserted to be washed in the blood bag, blood flows through the blood filtration filter I, and then flows into the leukocyte filter 3, white blood leukocytes trapped in the filter 3, and removing leukocytes before blood bag 4 into the washing, the red blood cells trapped within the film of the bag 41, the blood plasma flows into the plasma reservoir bag 5 bags permeable membrane 41, flows into the plasma when the plasma is fully pocket 5, the filter stopper leukocyte stream clip 12, the leukocyte filter 13 and flow stop clip plasma flow stop clamp 14 in the closed position, the only flow diversion pipe clamp 11 in the open position;

[0040] 三、将瓶塞穿刺器8插入洗涤液容器中,洗涤液流经血液过滤网1、导流管2,流入洗涤袋4的膜袋41内,并且边加洗涤液边摇动血袋42,使得洗涤液与膜袋41内的红细胞充分混合,将废液止流夹15置于打开位置,使得膜袋41内的洗涤废液完全流入废液收集袋6,将废液止流夹15置于关闭位置,如此形成一个清洗步骤,进一步洗去血浆蛋白,该步骤连续操作 [0040] Third, the Spikes washing liquid container 8 is inserted, the blood flow through the filter was washed with 1, the draft tube 2, flows into the bag 4 bag film 41 was washed, and shaken while adding wash liquid side bags 42, so that the red blood cells mixed with wash liquid 41 in the film bag, the waste stream retaining clip 15 in the open position, so that the washing waste liquid in the membrane pocket 41 completely into the waste collection bag 6, the waste stream retaining clip 15 in the closed position, thus forming a cleaning step, a plasma protein further wash, this step continuous operation

3、4次后,清洗完毕; After three or four times, cleaning is completed;

[0041 ]四、将瓶塞穿刺器8插入洗涤液容器中,洗涤液流入洗涤袋4的膜袋41内,并且边加洗涤液边摇动血袋42,使得洗涤液与膜袋41内的红细胞充分混合,关闭导流管止流夹11,打开红细胞止流夹16,拎起洗涤袋4,使得膜袋41内的红细胞流入红细胞储存袋7,打开导流管止流夹11,补加洗涤液,直至红细胞储存袋7内的血液容积达到标准规定,关闭红细胞止流夹16,洗涤红细胞制备完成。 [0041] Fourth, the Spikes washing liquid container 8 is inserted, the cleaning liquid flows into the bag 4 bag film 41 was washed, and shaken while adding wash liquid side bags 42, 41 such that the erythrocyte membrane pockets and wash liquor thoroughly mixed, the draft tube flow stop off clamp 11, clamp 16 is opened only flow of red blood cells, washed picked up bag 4, so that the erythrocyte membrane pockets 41 flows into the RBC storage bag 7, the draft tube flow stop opening clamp 11, additional washing solution, until the volume of red blood cells in the blood storage bag 7 reaches a predetermined criterion, only flow off erythrocytes clip 16, to complete the preparation of washed red blood cells.

[0042]实施例二:实施例二与实施例一区别在于:实施例一具有白细胞过滤器,因而具有过滤白细胞功能,可以去除血液中的白细胞。 [0042] Second Embodiment: Embodiment according to the second embodiment except that a: Example having a leucocyte filter, and thus has a filter function leukocytes, leukocytes in the blood may be removed. 对于已去除白细胞的血液或红细胞悬液,可以采用本实施例中的洗涤红细胞装置。 For blood leukocytes has been removed or red blood cells, red blood cells can be washed using the present apparatus in the embodiment.

[0043] 如附图2所示,一种洗涤红细胞装置,包括瓶塞穿刺器8、入管口与瓶塞穿刺器8相连接的血液过滤网1、入管口通过导流管2与血液过滤网I的出管口相连接的洗涤袋4、血浆储存袋5、废液收集袋6、入管口与洗涤袋4的入管,洗涤袋4的出管口、废液收集袋6的入管口、血浆储存袋5的入管口三者相连接,洗涤袋4包括血袋42、位于血袋42内腔中的能够透过血浆但不能透过红细胞的膜袋41,血袋42的入口边部、膜袋41的入口边部相连接,血浆储存袋5的入管设置有血浆止流夹14,废液收集袋6的入管设置有废液止流夹15,红细胞储存袋7的入管设置有红细胞止流夹16,导流管2上设置有导流管止流夹11。 [0043] As a red blood cell washing device 2, comprising a blood Spikes 8, and the nozzle 8 connected Spikes reference filter 1, the nozzle tube 2 and the blood flow through the filter washing the bag I of a nozzle 4 connected to the plasma storage bag 5, the waste liquid collecting bag 6, the spout washing the bag into the tube 4, washed with bags the spout 4, the waste collection bag into the spout 6, plasma, the storage bag spout 5 is connected to the three washing bag 4 comprises a blood bag 42, the blood bag 42 is located in the lumen but not permeable to the plasma 41, an inlet side portion 42 of the blood bag, the red blood cell membrane permeation membrane pockets the inlet side of the bag 41 is connected to the plasma storage bag into the pipe 5 is provided with a plasma flow stop clip 14, the waste collection bag into the pipe 6 is provided with a waste flow stop clip 15, red blood cell storage bag into the pipe 7 is provided with a red blood cell only flow clamp 16 is provided with a draft tube flow stop 11 on the clamp 2 of the draft tube.

[0044]所述的废液收集袋6装有空气过滤器。 [0044] The waste liquid collecting bag 6 equipped with an air filter. 所述的血袋42的左右两侧边部与所述的膜袋41的左右两侧边部相连接。 Left and right side portions of the blood bag 42 and the left and right side edge portions of the film 41 is connected to the bag. 血液过滤网I的入管口设置有瓶塞穿刺器8。 I into the blood filter is provided with a spout 8 Spikes. 血液过滤网1、导流管2、洗涤袋4、血浆储存袋5沿着从上到下的顺序分布设置。 The blood filter 1, the draft tube 2, washed bag 4, the distribution of the plasma storage bag 5 is provided along the top to bottom.

[0045] —种采用上述洗涤红细胞装置实现的洗涤红细胞制备方法,包括以下步骤: [0045] - seed preparation method of the washed red blood cells RBC washing apparatus implemented, comprising the steps of:

[0046] —、将瓶塞穿刺器8、血液过滤网1、导流管2、洗涤袋4、血浆储存袋5沿着从上到下的顺序分布设置,将导流管止流夹11与血浆止流夹14置于打开位置,将红细胞止流夹16与废液止流夹15都置于关闭位置; [0046] - the Spikes 8, the blood filter 1, the draft tube 2, washed bag 4, the plasma storage bag 5 is provided along a distribution order from the top, the guide pipe 11 and the retaining clip stream stopping the flow of plasma clamp 14 in the open position, the flow of red blood cells retaining clip 16 and the waste flow stop clip 15 are in the closed position;

[0047] 二、将瓶塞穿刺器8插入已去除白细胞的血液袋的输血插口中,血液流经血液过滤网I过滤,而后通过导流管2流入洗涤袋4,血液中的红细胞截留在膜袋41内,血液中的血浆透过膜袋41流入血浆储存袋5,当血浆完全流入血浆储存袋5后,将血浆止流夹14置于关闭位置; [0047] Second, the Spikes socket 8 is inserted into the removed blood leukocytes in the blood bag, blood flows through the blood filtration filter I, and then the bag into the washing tube 24 through flow, entrapping red blood cells in the membrane inner bag 41, the blood plasma flows into the plasma reservoir bag 5 bags permeable membrane 41, flows into the plasma when the plasma is fully pocket 5, the plasma flow stopper clamp 14 in the closed position;

[0048] 三、将瓶塞穿刺器8插入洗涤液容器中,洗涤液流入洗涤袋4的膜袋41内,并且边加洗涤液边摇动血袋42,使得洗涤液与膜袋41内的红细胞充分混合,将废液止流夹15置于打开位置,使得膜袋41内的洗涤废液完全流入废液收集袋6,将废液止流夹15置于关闭位置,如此形成一个清洗步骤,进一步洗去血浆蛋白,该步骤连续操作3、4次后,清洗完毕; [0048] Third, the Spikes washing liquid container 8 is inserted, the cleaning liquid flows into the bag 4 bag film 41 was washed, and shaken while adding wash liquid side bags 42, 41 such that the erythrocyte membrane pockets and wash liquor thoroughly mixed, the waste stream retaining clip 15 in the open position, so that the washing waste liquid in the membrane pocket 41 completely into the waste collection bag 6, the waste stream retaining clip 15 in the closed position, thus forming a cleaning step, washed further plasma proteins, after which 3,4 times step continuous operation, cleaning is completed;

[0049]四、将瓶塞穿刺器8插入洗涤液容器中,洗涤液流入洗涤袋4的膜袋41内,并且边加洗涤液边摇动血袋42,使得洗涤液与膜袋41内的红细胞充分混合,关闭导流管止流夹11,打开红细胞止流夹16,拎起洗涤袋4,使得膜袋41内的红细胞流入红细胞储存袋7,打开导流管止流夹11,补加洗涤液直至红细胞储存袋7内的血液容积达到标准规定,关闭红细胞止流夹16,洗涤红细胞制备完成。 [0049] Fourth, the Spikes washing liquid container 8 is inserted, the cleaning liquid flows into the bag 4 bag film 41 was washed, and shaken while adding wash liquid side bags 42, 41 such that the erythrocyte membrane pockets and wash liquor thoroughly mixed, the draft tube flow stop off clamp 11, clamp 16 is opened only flow of red blood cells, washed picked up bag 4, so that the erythrocyte membrane pockets 41 flows into the RBC storage bag 7, the draft tube flow stop opening clamp 11, additional washing RBC storage bag until the fluid volume in the blood reaches 7 standard, off the flow of red blood cells retaining clip 16, to complete the preparation of washed red blood cells.

[0050]如上所述,我们完全按照本发明的宗旨进行了说明,但本发明并非局限于上述实施例和实施方法。 [0050] As described above, we have fully been described in accordance with the spirit of the present invention, but the present invention is not limited to the above embodiments and a method embodiment. 相关技术领域的从业者可在本发明的技术思想许可的范围内进行不同的变化及实施。 Practitioners in the relevant art that various changes may be made in the embodiments within the scope and spirit of the invention permission.

Claims (10)

  1. 1.一种洗涤红细胞装置,其特征在于:包括瓶塞穿刺器(8)、入管口与所述的瓶塞穿刺器(8)相连接的血液过滤网(1)、入管口通过导流管(2)与所述的血液过滤网(I)的出管口相连接的洗涤袋(4)、血浆储存袋(5)、废液收集袋(6)、入管口与所述的洗涤袋(4)的入管口相连接的红细胞储存袋(7 ),所述的洗涤袋(4)的出管口、所述的废液收集袋(6 )的入管口、所述的血浆储存袋(5)的入管口三者相连接,所述的洗涤袋(4)包括血袋(42)、位于所述的血袋(42)内腔中的能够透过水溶液但不能透过红细胞的膜袋(41),所述的血袋(42)的入口边部与所述的膜袋(41)的入口边部相连接,所述的血浆储存袋(5)的入管设置有血浆止流夹(14),所述的废液收集袋(6)的入管设置有废液止流夹(15),所述的红细胞储存袋(7)的入管设置有红细胞止流夹(16),所述的导流管(2)上设置有导流管止流夹(11)。 An apparatus washed red blood cells, characterized by: a Spikes (8), a blood inlet tube and into said Spikes (8) connected to the filter (1), through the draft tube into the nozzle wash bag (4), the plasma storage bag (5), the waste collection bag (6), washed bag (into the spout (2) of the blood filter (I) is connected to the nozzle RBC storage bag (7) 4) is connected to the nozzle washing bag (4) out of the nozzle, the waste collection bag (6) into the nozzle, the plasma reservoir bag (5 ) of the nozzle is connected to three, the washed bag (4) comprises a blood bag (42), located in said blood bag (42) can pass through the lumen of an aqueous solution of erythrocytes but impermeable film bag ( 41), said blood bag (42) with an inlet side portion of the film bag (41) is connected to an inlet edge portion, the plasma storage bag (5) into the plasma tube is provided with flow stop clip (14 ), the waste collection bag (6) into the waste pipe is provided with a retaining clip stream (15), said red blood cell storage bag (7) into the tube is provided with a red blood cell flow stop clip (16), said guide the flow tube is provided with only flow diversion pipe clamp (11) (2).
  2. 2.根据权利要求1所述的洗涤红细胞装置,其特征在于:所述的废液收集袋(6)装有空气过滤器。 2. The apparatus according to washed red blood cells according to claim 1, wherein: the waste collection bag (6) equipped with an air filter.
  3. 3.根据权利要求1所述的洗涤红细胞装置,其特征在于:所述的血袋(42)的左右两侧边部与所述的膜袋(41)的左右两侧边部相连接。 3. The apparatus according to washed red blood cells according to claim 1, wherein: said blood bag (42) of the left and right side edge portions of the film bag (41) is connected to the left and right side portions.
  4. 4.根据权利要求1所述的洗涤红细胞装置,其特征在于:所述的瓶塞穿刺器(8)、所述的血液过滤网(I)、所述的导流管(2)、所述的洗涤袋(4)、所述的血浆储存袋(5)沿着从上到下的顺序分布设置。 4. The apparatus of washed red blood cells according to claim 1, wherein: said Spikes (8), said blood filter (the I), the draft tube (2), the wash bag (4), the plasma storage bag (5) arranged distributed along the order from the top.
  5. 5.—种洗涤红细胞装置,其特征在于:包括瓶塞穿刺器(8)、入管口与所述的瓶塞穿刺器(8)相连接的血液过滤网(1)、入管口通过导流管(2)与所述的血液过滤网(I)的出管口相连接的洗涤袋(4)、白细胞过滤器(3)、血浆储存袋(5)、废液收集袋(6)、入管口与所述的洗涤袋(4)的入管相连接的红细胞储存袋(7),所述的白细胞过滤器(3)的入管口、所述的导流管(2)的入管口与所述的血液过滤网(I)的出管口相连接,所述的导流管(2)的出管口、所述的白细胞过滤器(3)的出管口与所述的洗涤袋(4)的入管口相连接,所述的白细胞过滤器(3)与所述的导流管(2)相并列的设置,所述的洗涤袋(4)的出管口、所述的废液收集袋(6)的入管口、所述的血浆储存袋(5)的入管口三者相连接,所述的洗涤袋(4)包括血袋(42)、位于所述的血袋(42)内腔中的能够透过水溶液但不能透过红细胞的膜袋(41),所述 5.- species apparatus washed red blood cells, characterized by: a Spikes (8), a blood inlet tube and into said Spikes (8) connected to the filter (1), through the draft tube into the nozzle wash bag (2) of the blood filter (I) is connected to the nozzle (4), leukocyte filter (3), the plasma storage bag (5), the waste collection bag (6), into the mouthpiece RBC storage bag (7) is washed with said bags (4) into a tube connected to said leukocyte filter (3) into the nozzle, the draft tube (2) into the said spout and blood filter (I) is connected to the nozzle, the draft tube (2) of the nozzle, the leukocyte filter tube (3) of the port and the wash bag (4) the nozzle is connected leukocyte filter (3) and the said draft tube (2) provided with parallel, washed bag (4) out of the nozzle, the waste collection bag ( 6) into the nozzle, the nozzle three plasma reservoir bag (5) is connected to the said wash bag (4) comprises a blood bag (42), located in said blood bag (42) in the lumen but it can be an aqueous solution of the red blood cells through the impermeable membrane pockets (41), 血袋(42)的入口边部与所述的膜袋(41)的入口边部相连接,所述的血浆储存袋(5)的入管设置有血浆止流夹(14),所述的废液收集袋(6)的入管设置有废液止流夹(15),所述的红细胞储存袋(7)的入管设置有红细胞止流夹(16),所述的导流管(2)上设置有导流管止流夹(11),所述的白细胞过滤器(3)的入管上设置有白细胞过滤前止流夹(12),所述的白细胞过滤器(3)的出管上设置有白细胞过滤后止流夹(13)。 Blood bag (42) with an inlet side portion of the film bag (41) is connected to an inlet edge portion, the plasma storage bag (5) into the plasma tube is provided with flow stop clip (14), the waste liquid collection bag (6) into the waste pipe is provided with a retaining clip stream (15), said red blood cell storage bag (7) into the tube is provided with a red blood cell flow stop clip (16), the draft tube (2) is provided with a draft tube flow stop clip (11), provided with a front stop stream clip leukocyte filter (12) on leukocyte filter into the tube (3) according to, the tube is provided on the leukocyte filter (3) of the after filtering leukocytes flow stop clip (13).
  6. 6.根据权利要求5所述的洗涤红细胞装置,其特征在于:所述的废液收集袋(6)装有空气过滤器。 6. The apparatus of washed red blood cells according to claim 5, characterized in that: said waste collection bag (6) equipped with an air filter.
  7. 7.根据权利要求5所述的洗涤红细胞装置,其特征在于:所述的血袋(42)的左右两侧边部与所述的膜袋(41)的左右两侧边部相连接。 7. The apparatus of washed red blood cells according to claim 5, wherein: said blood bag (42) of the left and right side edge portions of the film bag (41) is connected to the left and right side portions.
  8. 8.根据权利要求5所述的洗涤红细胞装置,其特征在于:所述的瓶塞穿刺器(8)、所述的血液过滤网(I)、所述的白细胞过滤器(3)、所述的洗涤袋(4)、所述的血浆储存袋(5)沿着从上到下的顺序分布设置。 8. The apparatus of washed red blood cells according to claim 5, wherein: said Spikes (8), said blood filter (the I), the leukocyte filter (3), the wash bag (4), the plasma storage bag (5) arranged distributed along the order from the top.
  9. 9.一种采用权利要求1所述的洗涤红细胞装置实现的洗涤红细胞制备方法,其特征在于:包括以下步骤: 一、将所述的瓶塞穿刺器(8)、所述的血液过滤网(1)、所述的导流管(2)、所述的洗涤袋(4)、所述的血浆储存袋(5)沿着从上到下的顺序分布设置,将所述的导流管止流夹(11)与所述的血浆止流夹(14)置于打开位置,将所述的红细胞止流夹(16)与所述的废液止流夹(15)都置于关闭位置; 二、将所述的瓶塞穿刺器(8)插入已去除白细胞的血液袋的输血插口中,血液流经所述的血液过滤网(I)过滤,而后通过所述的导流管(2)流入所述的洗涤袋(4),血液中的红细胞截留在所述的膜袋(41)内,血液中的血浆透过所述的膜袋(41)流入所述的血浆储存袋(5),当血浆完全流入所述的血浆储存袋(5)后,将所述的血浆止流夹(14)置于关闭位置; 三、将所述的瓶塞穿刺器(8)插入洗涤液容器中 A method of using the preparation of claim washed red blood cells washed red blood cells-implemented apparatus of claim 1, characterized in that: comprising the steps of: a, the piercing of the stopper (8), said blood filter ( 1), the draft tube (2), the washing bag (4), the plasma storage bag (5) arranged distributed along the top to bottom of the draft tube stop stream clip (11) with said plasma flow stopping clip (14) in the open position, the flow of red blood cells stop clip (16) with the waste stream stop clip (15) are in the closed position; Second, the piercing of the stopper (8) is inserted into the socket has been removed blood leukocytes in the blood bag, blood flowing through the blood filter (I) was filtered, then through the draft tube (2) flowing into said wash bags (4), red blood cells trapped within the membrane pockets (41), blood plasma (41) flows through the plasma membrane pocket said storage bag (5) , when the plasma completely into said plasma storage bag (5), the plasma flow stopping clip (14) in the closed position; Third, the piercing of the stopper (8) into the washing liquid container 洗涤液流入所述的洗涤袋(4)的膜袋(41)内,并且边加洗涤液边摇动所述的血袋(42),使得洗涤液与所述的膜袋(41)内的红细胞充分混合,将所述的废液止流夹(15)置于打开位置,使得所述的膜袋(41)内的洗涤废液完全流入所述的废液收集袋(6),将所述的废液止流夹(15)置于关闭位置,如此形成一个清洗步骤,该步骤连续操作1-6次后,清洗完毕; 四、将所述的瓶塞穿刺器(8)插入洗涤液容器中,洗涤液流入所述的洗涤袋(4)的膜袋(41)内,并且边加洗涤液边摇动所述的血袋(42),使得洗涤液与所述的膜袋(41)内的红细胞充分混合,关闭所述的导流管止流夹(11),打开所述的红细胞止流夹(16),拎起所述的洗涤袋(4),使得所述的膜袋(41)内的红细胞流入所述的红细胞储存袋(7),打开所述的导流管止流夹(11 ),补加洗涤液直至所述的红细胞储存袋(7)内的血液容积达到标准规 Washed erythrocyte bag (4) of the cleaning liquid flowing into the film bag (41), and shaking while adding wash liquid blood bag edge (42), such that the washing liquid and the membrane pockets (41) in the thoroughly mixed, the waste stream retaining clip (15) in the open position, such that the membrane pockets (41) of washing liquid waste flows completely within the waste collection bag (6), the after the waste stream locking clip (15) in the closed position, thus forming a cleaning step, this step 1-6 times continuous operation, cleaning is completed; Fourth, the piercing of the stopper (8) is inserted into the cleaning liquid container , the washing liquid flowing into the washing bag (4) of the membrane pockets (41), and while adding blood bag (42) of the rocking edge of the cleaning liquid, so that (41) of the membrane pocket with said washing liquid red blood cells mixed closing the flow stop draft tube clamp (11), opening the flow of red blood cells retaining clip (16), picked up the wash bag (4), such that the membrane pockets (41 RBC) flowing into the red blood cell storage bag (7), opening the clamp only flow diversion pipe (11), the blood volume in the additional wash liquid until the red blood cell storage bag (7) up to standard gauge ,关闭所述的红细胞止流夹(16),洗涤红细胞制备完成。 Closing the erythrocytes retaining clip stream (16), to complete the preparation of washed red blood cells.
  10. 10.—种采用权利要求5所述的洗涤红细胞装置实现的洗涤红细胞制备方法,其特征在于:包括以下步骤: 一、所述的瓶塞穿刺器(8)、所述的血液过滤网(1)、所述的白细胞过滤器(3)、所述的洗涤袋(4)、所述的血浆储存袋(5)沿着从上到下的顺序分布设置,将所述的白细胞过滤前止流夹(12)、所述的白细胞过滤后止流夹(13)与所述的血浆止流夹(14)置于打开位置,将所述的导流管止流夹(11)、所述的红细胞止流夹(16)与所述的废液止流夹(15)都置于关闭位置; 二、将所述的瓶塞穿刺器(8)插入待洗涤血液袋的输血插口中,血液流经所述的血液过滤网(I)过滤,而后流入所述的白细胞过滤器(3),血液中的白细胞截留在所述的白细胞过滤器(3)内,然后去除白细胞的血液流入所述的洗涤袋(4),血液中的红细胞截留在所述的膜袋(41)内,血液中的血浆透过所述的膜袋(41)流入所述的 Preparation washed red blood cells RBC washing device realized 10.- species employed according to claim 5, characterized in that: comprising the steps of: a said Spikes (8), said blood filter (1 ), the leukocyte filter (3), the washing bag (4), the plasma storage bag (5) arranged distributed along the order from the top, before the stop flow leukocyte filter clamp (12), said leukocyte filter after the flow stop clip (13) with said plasma flow stopping clip (14) in the open position, the flow of the draft tube locking clamp (11), said RBC flow stop clip (16) with the waste stream stop clip (15) are in the closed position; two, the piercing of the stopper (8) to be inserted into the bags of blood transfusion washed socket, blood flow through the blood filter (I) was filtered, and then flows into the leukocyte filter (3), wherein, white blood leukocytes trapped in the filter (3), and then removing the inflow of blood leukocytes wash bag (4), red blood cells trapped within the membrane pockets (41), blood plasma (41) into said pocket through said membrane 血浆储存袋(5),当血浆完全流入所述的血浆储存袋(5)后,将所述的白细胞过滤前止流夹(12)、所述的白细胞过滤后止流夹(13)与所述的血浆止流夹(14)置于关闭位置,将所述的导流管止流夹(11)置于打开位置; 三、将所述的瓶塞穿刺器(8)插入洗涤液容器中,洗涤液流经所述的血液过滤网(1)、所述的导流管(2),流入所述的洗涤袋(4)的膜袋(41)内,并且边加洗涤液边摇动所述的血袋(42),使得洗涤液与所述的膜袋(41)内的红细胞充分混合,将所述的废液止流夹(15)置于打开位置,使得所述的膜袋(41)内的洗涤废液完全流入所述的废液收集袋(6),将所述的废液止流夹(15)置于关闭位置,如此形成一个清洗步骤,该步骤连续操作1-6次后,清洗完毕; 四、将所述的瓶塞穿刺器(8)插入洗涤液容器中,洗涤液流入所述的洗涤袋(4)的膜袋(41)内,并且边加洗涤液边摇动所述的血袋 Plasma storage bag (5), when the plasma completely into said plasma storage bag (5), the flow stop before filtering leukocytes clamp (12), said leukocyte filter after the flow stop clip (13) and the said plasma flow stop clip (14) in the closed position, the flow of the draft tube locking clamp (11) in the open position; Third, the piercing of the stopper (8) into the washing liquid container washing of the blood flow through the filter (1), the draft tube (2), wash the bag (4) flowing into the film bag (41), and the wash liquid while adding the rocking edge blood bag (42) described later, so that the membrane pockets and wash liquor mixed in erythrocytes (41), the waste stream locking clip (15) in the open position, such that said film bags ( washing waste liquid 41) completely into said waste collection bag (6), the waste stream locking clip (15) in the closed position, thus forming a cleaning step of continuous operation 1-6 after time, cleaning is completed; Fourth, the piercing of the stopper (8) is inserted into the cleaning liquid container, wash bag (4) of the cleaning liquid flowing into the film bag (41), and while adding wash liquid side shaking said blood bag (42),使得洗涤液与所述的膜袋(41)内的红细胞充分混合,关闭所述的导流管止流夹(11),打开所述的红细胞止流夹(16),拎起所述的洗涤袋(4),使得所述的膜袋(41)内的红细胞流入所述的红细胞储存袋(7),打开所述的导流管止流夹(11),补加洗涤液,直至所述的红细胞储存袋(7)内的血液容积达到标准规定,关闭所述的红细胞止流夹(16),洗涤红细胞制备完成。 (42), such that said film bags washing liquid mixed within erythrocytes (41), closing the flow stop flow tube clamp (11), opening the flow of red blood cells retaining clip (16), picked up washing said bag (4), such that the erythrocyte membrane pockets (41) into the red blood cell storage bag (7), opening the clamp only flow diversion pipe (11), additional washing liquid , until the blood volume in the red blood cell storage bag (7) reaches a predetermined standard, off the flow of red blood cells retaining clip (16), to complete the preparation of washed red blood cells.
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CN1590540A (en) * 2004-05-24 2005-03-09 乔泽江 Method and device for preparing washing erythrocyte and freezing deglycerin erythrocyte
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