CN1264568C

CN1264568C - 毒力基因和蛋白质及其用途

Info

Publication number: CN1264568C
Application number: CNB2003101025116A
Authority: CN
Inventors: H·R·克鲁克; E·E·克拉克; P·H·艾沃莱斯特; G·杜甘; D·W·霍尔登; J·E·施艾; R·G·菲尔德曼
Original assignee: Microscience Ltd
Current assignee: Emergent Product Development UK Ltd
Priority date: 1998-11-09
Filing date: 1999-11-09
Publication date: 2006-07-19
Anticipated expiration: 2019-11-09
Also published as: EP1129196A2; AU773003B2; ATE383425T1; EP1914312A2; AU1060500A; DE69937965D1; EP1129196B1; US20050142149A1; DE69937965T2; EP1914312A3; WO2000028038A2; CN1524575A; NZ527279A; US6846667B1; NZ511366A; KR20010099789A; BR9915165A; WO2000028038A3; CN1196787C; CN1330714A

Abstract

本发明以鉴定一系列大肠杆菌K1中的毒力基因为基础，其产物可能与生物体的致病性有关。对该基因的鉴定使得可以将它们或其表达的产物以许多方式用于治疗感染。

Description

毒力基因和蛋白质及其用途

本申请是国际申请日为1999年11月9日，中国申请号为99814336.7发明创造名称同上的申请的分案申请。

发明领域

本发明涉及毒力基因和蛋白质的鉴定及其用途。更具体地说，本发明涉及它们在治疗和筛选药物中的用途。

发明背景

大肠杆菌属于肠杆菌科或肠道细菌，它们是聚居在动物肠道中的革兰氏阴性微生物。该细菌科中的其它细菌包括肠杆菌属、克雷伯氏菌属、沙门氏菌属、志贺氏菌属和耶尔森氏菌属。尽管通常在人胃肠道中发现大肠杆菌，但是它与包括败血病、脑膜炎、尿道感染、伤口感染、脓肿形成、腹膜炎和胆管炎在内的人体疾病有关。

由大肠杆菌导致的疾病取决于某些毒力决定簇。例如，大肠杆菌与新生儿脑膜炎有关并已将主要的毒力决定簇鉴定为K1抗原，它是唾液酸的均聚物。K1抗原可以在回避宿主免疫系统和防止吞噬作用方面起作用。

发明概要

本发明以一系列大肠杆菌K1中的毒力基因的鉴定以及相关的生物体为基础，其产物可能与生物体的致病性有关。

本发明的一个方面是一种由操纵子编码的肽、或其在革兰氏阴性菌中的同源物或其功能片段，其中所述的操纵子包括来自大肠杆菌K1的本文鉴定为mdoG、creC、recG、yggN、tatA、tatB、tatC、tatE、eck1、iroD、iroC、iroE、mtd2和ms1-16在内的任意基因。例如，这类肽在分离时适合于治疗用途。

本文所用的术语“功能片段”指的是保留与完整基因或肽类似的治疗用途的一部分基因或肽。例如，可以将所述肽的功能片段用作在疫苗或生产抗体过程中有用的抗原决定簇。可以将基因片段用于编码活性肽。另一方面，该基因片段可能在基因疗法中有用，从而靶向体内的野生型基因以便发挥治疗作用。

本发明的肽可以包括本文鉴定为SEQ ID NOS.2、5、7、9、11、12、13、14、16、23、24、25、26、28、31、29、32和35-48的任意氨基酸序列。

将这些肽类鉴定为毒力决定簇使得能够将它们以许多方式用于治疗感染。例如，可以将宿主转化以表达本发明的肽或将所述宿主进行修饰以便破坏编码所述肽的基因的表达。一种疫苗还可以包括用于治疗感染的本发明的肽或用于其表达的工具。此外，疫苗可以包括具有毒力基因缺失的微生物，其中所述的基因编码本发明的肽。

根据本发明的另一个方面，可以将所述肽类或基因用于筛选有潜能的抗微生物药或用于检测毒性。

本发明的另一个方面是本文所鉴定的任意产物在治疗或预防与革兰氏阴性菌、特别是大肠杆菌导致的感染有关的疾病中的用途。

发明描述

本发明利用了特征标记的诱变(STM)(Hensel等《科学》(Science)1995；269：400-403)以便筛选减毒突变体的大肠杆菌K1菌株RS228(Pluschke等《感染与免疫》(Infection and Immunity)39：599-608)小Tn5突变体库，从而鉴定了大肠杆菌的毒力基因(和毒力决定簇)。

尽管将大肠杆菌K1用作鉴定毒力基因的微生物，但是也将其它肠道细菌中的相应基因看作属于本发明的范围。例如，以序列同源性为基础，可以在肠杆菌属、克雷伯氏菌属和包括沙门氏菌属、志贺氏菌属和耶尔森氏菌属在内的与人体肠道疾病相关的其它属中发现相应的基因或编码的蛋白质。

本文所用的术语“毒力决定簇”指的是一种产物，例如一种可能起维持病原菌作用的肽或蛋白质。特别地，毒力决定簇是一种与感染性或导致疾病的微生物有关的细菌蛋白质或肽。

可以将编码毒力决定簇的基因称作“毒力基因”。通过突变、缺失或插入破坏毒力基因会导致细菌在宿主中的存活率降低或通常导致微生物致病性的降低。

已经证实特征标记的诱变是一种用于鉴定毒力基因及其产物的极为有用的技术。该项技术依赖于转座子在允许条件下随机插入微生物基因组中的能力。将转座子分别标记以便于鉴定且然后分别引入微生物，从而使基因组受到破坏。接着通过阴性选择来检测毒力降低的突变型微生物并对已经发生插入失活的基因进行鉴定和特征记述。

STM过程中的第一个阶段是制备合适的转座子或转座子样元件。制备不同转座子文库，将它们各自引入载体或质粒以促进其转入微生物。本领域技术人员显然可以制备带有合适转座子的载体且这项技术进一步公开在WO-A-96/17951中。对于革兰氏阴性菌例如大肠杆菌来说，合适的转座子包括Tn5和Tn10。在制备了转座子后，则对细菌菌株进行诱变以便建立各突变型细菌的文库。

接着将收集的突变型微生物引入合适的宿主。在适当长的时间后，从宿主中回收微生物并鉴定已经在宿主中存活的那些微生物，由此还鉴定了不能存活的突变型菌株即减毒株。然后将储存文库中相应的减毒株用于鉴定发生插入失活的基因。通常，通过分离位于转座子插入位点侧翼的DNA来鉴定转座子插入的位点且这使得对与毒力相关的基因进行特征记述成为可能。

一旦鉴定了无毒性微生物，则能够通过用致死剂量的突变体感染合适的宿主动物而更充分地确定突变型基因在毒力中的潜在作用。将受感染动物的存活时间与用野生型菌株感染的对照组动物的存活时间进行比较且一般可以认为存活期限比对照组动物长的那些动物感染了带有突变型毒力基因的微生物。

另一方面，通过用野生型和突变体细菌的混合物感染动物宿主可以研究毒力中的潜在作用。在合适的时间期限后，从宿主动物的器官中收集细菌并测定野生型和突变体细菌的比例。将该比例除以接种物中突变体与野生型细菌的比例来确定竞争指数(CI)。可以认为具有竞争指数小于1的突变体是无毒性的。

有可能通过插入转座子而失活的基因可能不是真正的毒力基因，但是可以对下游(毒力)基因具有极性作用。这可以通过进一步的实验来确定，所述的实验过程包括使非极性突变置于基因的更为确定的区域或使其它邻近基因发生突变并确定该突变体是否是无毒性的。

由于对大肠杆菌中的毒力基因进行了特征记述，所以有可能使用用来确定其它微生物中同源性的基因序列。以这种方式，有可能确定其它微生物是否具有相似的毒力决定簇。通过检索例如EMBL或Genbank这样的现存的数据库可以确定序列同源性。

通常使毒力基因在称作致病性岛的不同染色体区中彼此群集。当致病性岛通常位于重复序列、插入元件或tRNA基因侧翼时，可以识别它们。另外，G+C含量一般不同于染色体的剩余物，这提示它们通过从另一种生物体中的水平传递而获得。例如，大肠杆菌K12的G+C含量为52％。在大肠杆菌菌株中发现的任意致病性岛可能具有不同于该平均值的G+C含量。

经鉴定的毒力基因能够用于生产减毒的疫苗菌株和作为抗微生物药的靶物。一般来说，这对于革兰氏阴性菌中的同源物来说同样确切。

为了本发明的目的，同源性的适当程度一般至少为30％，优选至少为50％、60％或70％，且更优选至少为80％或90％(在氨基酸或核苷酸水平上)。

通过本领域中已知的方法可以纯化和分离本发明的蛋白质。特别地，由于鉴定了基因序列，所以有可能使用重组技术在合适的宿主中表达基因。可以鉴定活性片段和同源物并可以将它们用于治疗中。例如，可以将蛋白质或其活性片段用作疫苗中的抗原决定簇以便引起免疫反应。还可以将它们用于制备被动免疫用抗体或用于诊断应用。合适的抗体包括单克隆抗体或包括单链fv片段在内的其片段。抗体的制备方法对于本领域技术人员来说是显而易见的。

以减毒微生物为基础的疫苗制品对本领域技术人员来说是已知的。如果必要或需要，可以用例如明矾这样的合适的载体或佐剂来配制疫苗组合物并可以将它们用于治疗，从而提供对大肠杆菌或其它革兰氏阴性菌的有效免疫接种。疫苗制品的制备方法对于本领域技术人员来说是显而易见的。

更为常见的和正如本领域技术人员众所周知的，当可以含有合适的载体或赋形剂时，可以对本发明活性成分的治疗用合适量和给药途径进行选择。根据诸如所治疗疾病的性质/严重程度、患者的类型或健康情况等这样的已知标准来选择或决定这些因素。

下列实施例解释了本发明。对于这些实施例来说，使用全身感染的小鼠模型将STM用于筛选减毒突变体的大肠杆菌K1小Tn5突变体库。该实验方法的基本步骤公开在Hensel等的上述文献中。未从接种了混合型突变体群体的小鼠中回收到毒力基因内含有小Tn5插入物的大肠杆菌K1且因此该大肠杆菌K1可能是被减毒的。

通过反向PCR或通过拯救卡那霉素抗性标记克隆了位于小Tn5插入物各侧翼的DNA区。在后一种情况下，将来自STM衍生的突变体的染色体DNA用限制酶消化、连入质粒pUC19并在转化入感受态大肠杆菌K12细胞后选择卡那霉素抗性克隆。然后进行随后的克隆和测序步骤并使用可公开得到的序列数据库(EMBL)中的序列与所述基因序列进行比较以便辅助进行对推定基因产物的特征记述。

实施例1

在第一种突变体中，对所克隆的DNA的两个片段进行测序。将核苷酸序列表示为SEQ ID NO.1和SEQ ID NO.3并将来自SEQ ID NO.1的DNA翻译区表示为SEQ ID NO.2。SEQ ID NO.1显示出与来自大肠杆菌K12(EMBL数据库登记号AE000206)的mdoGH区的2577-6908位核苷酸具有99.8％的同一性。这种DNA片段编码ymdD基因的5’部分、完整的mdoG基因和mdoH基因的5’部分。mdoG基因产物的功能是未知的，不过，认为它与膜衍生的寡糖类的生物合成有关。

SEQ ID NO.3显示出与来自大肠杆菌K12的mdoGH基因的3’部分和下游基因序列(7187-7760位核苷酸)具有98.3％的同一性。SEQID NO.2显示出与来自大肠杆菌K12(Swiss Prot登记号P33136)的mdoG蛋白质在1-511位氨基酸上具有99.6％的同一性。

在全身感染的小鼠模型体内测试新型基因对使用混合型感染的毒力的减毒作用(Achtman等《感染与免疫》(Infection and Immunity)1983；第39卷：315-335)并证实以0.38的竞争指数被减毒。这证实原始转座子突变体的减毒可能是因mdoG基因受到破坏而导致的。

构建mdoG的极性和非极性缺失突变体。通过使用寡核苷酸5’-TGCTCTAGAGCCATTACTCAGAATGGG-3’(SEQ ID NO.49)和5’-CGCGAGCTCGACGACTGAATGATCCC-3’(SEQ ID NO.50)进行PCR来扩增mdoG基因和侧翼区。将产物克隆入pUC19。然后通过使用寡核苷酸5’-TCCCCCGGGTACTGCAGCACTCAACC-3’(SEQ ID NO.51)和5’-GATCCCGGGACCACTGAAATGCGTGC-3’(SEQ ID NO.52)进行反向PCR来扩增含有mdoG的5’-和3’-末端片段和完整pUC19序列的PCR产物。将非极性卡那霉素抗性弹夹(aphT)沿两个方向插入mdoG序列之间而得到极性和非极性构建体。接着将mdoG∷aphT融合体转入自杀载体pCDV442。通过将pCDV442构建体连入野生型大肠杆菌K1后的等位转移使mdoG的染色体拷贝发生突变。

测试所构建的突变体在全身感染小鼠模型体内对毒力的减毒作用(Achtman等，文献同上)。分别以0.37和0.35的竞争指数(平均值C1来自三只小鼠)使极性和非极性构建体的毒力得到弱化。这证明原始转座子突变体的减毒可能是因mdoG基因受到破坏而导致的。

实施例2

用具有以SEQ ID NO.4表示的核苷酸序列和表示为SEQ ID NO.5的翻译的氨基酸序列的毒力基因鉴定第二种突变体。小Tn5转座子插在581位核苷酸(SEQ ID NO.4)和187位氨基酸(SEQ ID NO.5)上。

这些序列显示出与大肠杆菌K12(EMBL和Genbank登记号M13608、AE000510和U14003)的creC基因具有97.9％的同一性。

来自大肠杆菌K12的creC蛋白质属于组氨酸激酶的蛋白质族且属于由含信号结构域的蛋白质组成的蛋白质族。

测试新型基因对毒力的减毒作用(Achtman等，文献同上)且证实该新型基因是以0.09的竞争指数被减毒的。

当将大肠杆菌K12 creC基因转录成带有creD基因的操纵子的一部分时，这种减毒作用可能是因对推定大肠杆菌K1 creD基因的极性作用所导致的。

实施例3

第三种突变体在紧接小Tn5后带有表示为SEQ ID NO.6的核苷酸序列。将翻译的该序列表示为SEQ ID NO.7。

该核苷酸序列显示出与大肠杆菌K12(EMBL和Genbank登记号P24230和M64367)recG基因的5-146位核苷酸具有93.7％的同一性。这表明受到破坏的基因至少部分与大肠杆菌K12的recG基因相同。大肠杆菌K12的recG基因编码起ATP依赖性DNA解旋酶作用的76.4kD蛋白质并在DNA修复过程中起关键作用。

在减毒试验中，证实竞争指数为0.48。将recG基因转录成操纵子的末端基因且因此由于对另一种大肠杆菌K1基因产生极性作用而导致这种减毒作用是不可能的。

实施例4

如果翻译产物表示为SEQ ID NO.9，那么第四种突变体带有在表示为SEQ ID NO.8的核苷酸序列内插入的转座子。

小Tn5转座子插在359位核苷酸和80位氨基酸上。

这些序列显示出与大肠杆菌K12(EMBL登记号AE000378)的yggN基因的339-1054位核苷酸具有98.5％的序列同一性且在氨基酸水平上具有99.6％的同一性。

尽管yggN基因的序列是已知的，但是其编码的蛋白质的功能尚未确定。

测试新型基因对毒力的减毒作用且证实该新型基因是以0.43的竞争指数被减毒的。

实施例5

通过在相同区内插入转座子还发现了几种突变体。对该区的克隆和测序显示了表示为SEQ ID NO.10的核苷酸序列。该序列与大肠杆菌K12(EMBL和Genbank登记号AJ005830、AE000459和AE000167)的tatABCD操纵子具有同源性。该操纵子编码推定质量为9.6kD、18.4kD、28.9kD和29.5kD的蛋白质，它们起Sec依赖性蛋白质输出途径中成分的作用。在细胞质中结合辅因子后，该途径使全折叠的蛋白质通过门控孔移位至周质。

对核苷酸序列的翻译显示了相当于tatA的蛋白质(SEQ IDNO.11)、相当于tatB的序列(SEQ ID NO.12)、相当于tatC的序列(SEQ ID NO.13)和相当于tatD的序列(SEQ ID NO.14)。

由STM鉴定的突变体中的小Tn5转座子定位于SEQ ID NO.10的1429和2226位核苷酸上。这些转座子的插入破坏了tatB蛋白质序列的50位氨基酸和tatC蛋白质序列的143位氨基酸。

测试tatB和tatC基因对毒力的减毒作用且证实这些基因分别以0.0012和0.0039的竞争指数被减毒。当在全身感染的相同模型中测试单一感染时，这些基因在毒性上也发生了弱化。

实施例6

在相当于表示为SEQ ID NO.15的大肠杆菌K12的tatE基因的区内通过插入方式使另一种突变体失活。该序列的翻译如SEQ ID NO.16所示。tatE基因显示出与大肠杆菌K12基因(登记号AE000167)的6719-7306位核苷酸具有98％的同一性。

为了确定tatA、tatD和tatE基因是否需要毒性，在它们各自中构建非极性缺失突变。使用下列引物扩增位于各tatA、tatD和tatE基因侧翼的DNA区：

tatA

5’-TCG TCT AGA GAT GAT GGT GAT GGA GCG-3’(SEQ ID NO.53)

5’-GAA CTG CAG CCA AAT ACT GAT ACC ACC C-3’(SEQ ID NO.54)

5’-GAA CTG CAG GCT AAA ACA GAA GAC GCG-3’(SEQ ID NO.55)

5’-CAT GCA TGC ACT CCA TAT GAC AAC CGC-3’(SEQ ID NO.56)

将引物SEQ ID NO.53和SEQ ID NO.54用于扩增tatA上游的DNA序列；将引物SEQ ID NO.55和SEQ ID NO.56用于扩增tatA下游的DNA序列。

tatD

5’-TCG TCT AGA ATG AAG CTG CGC ATG AGG-3’(SEQ ID NO.57)

5’-CAA CTG CAG TCG CAA ATT GCG AAC TGG-3’(SEQ ID NO.58)

5’-CAA CTG CAG ACC GCA ACT TTT CGA CGC-3’(SEQ ID NO.59)

5’-CAT GCA TGC CAG TGA GCC ATT GTT CCC-3’(SEQ ID NO.60)

将引物SEQ ID NO.57和SEQ ID NO.58用于扩增tatD上游的DNA序列；将引物SEQ ID NO.59和SEQ ID NO.60用于扩增tatD下游的DNA序列。

tatE

5’-TGC TCT AGA TAC GAC TCT GAC AGG AGG-3’(SEQ ID NO.61)

5’-TCA GAT ATC AAC TAC CAG CAG TTT GG-3’(SEQ ID NO.62)

5’-TCA GAT ATC CAT AAA GAG TGA CGT GGC-3’(SEQ ID NO.63)

5’-TGC TCT AGA AAA CGT GGC AAC AGA GCG-3’(SEQ ID NO.64)

将引物SEQ ID NO.61和SEQ ID NO.62用于扩增tatE上游的DNA序列；将引物SEQ ID NO.63和SEQ ID NO.64用于扩增tatE下游的DNA序列。

在将这些侧翼DNA片段克隆入pUC19中后，将非极性aphT卡那霉素抗性弹夹(Galan等《细菌学杂志》(J.Bacteriol)1992；174：4338-4349)插入侧翼DNA片段之间以便取代tatA、tatD和tatE基因。然后将这些DNA片段转入自杀载体pCVD442(Blomfield等《分子微生物学》(Mol.Micro.)1991；5：1447-1457)。接着通过将pCVD442构建体连入野生型大肠杆菌K1后的等位转移使大肠杆菌K1tatA、tatD和tatE基因的染色体拷贝发生突变。

对受到破坏的tatA、tatD和tatE基因测试对毒力的减毒作用(Achtman等，文献同上)。

没有一种基因在分离过程中缺失时被减毒。这些基因仍然可在毒性中起作用，且为了测试这种情况，通过在tatA和tatE基因中发生缺失来制备突变体。对双重突变体测试经与野生型菌株的混合型感染的毒力的减毒作用且证明该双重突变体以0.0017的竞争指数被减毒。因此看来可以混合使用tatA、tatD和tatE基因以便产生无毒性微生物。

由于大肠杆菌K1 tatABCD基因与存在于鼠伤寒沙门氏菌基因组和脑膜炎奈瑟氏球菌基因组中的推定tatABCD基因具有相似性，所以看起来可能的是在这些和其它生物体中tat系统也可能需要毒性。通过用下列引物扩增位于tatC基因侧翼的DNA来构建鼠伤寒沙门氏菌tatC基因(SEQ ID NO.17)中的缺失：

5’-TGC TCT AGA AGG CGT TGT CGA TCC TG-3’(SEQ ID NO.65)

5’-GAA CTG CAG GAA AAG GCC GAG CAG ACT G-3’(SEQ ID NO.66)

5’-GAA CTG CAG TAC AGC CAT GTT TAC GGT-3’(SEQ ID NO.67)

5’-CAT GCA TGC GGT GTA CGA CAG TTT GCG-3’(SEQ ID NO.68)

将引物SEQ ID NO.65和SEQ ID NO.66用于扩增鼠伤寒沙门氏菌tatC基因下游的DNA序列；将引物SEQ ID NO.67和SEQ ID NO.68用于扩增鼠伤寒沙门氏菌tatC基因上游的DNA序列。

将所编码的tatC基因的两个区的氨基酸序列表示为SEQ ID NO.18和SEQ ID NO.19。

在将这些侧翼DNA片段克隆入pUC19中后，将非极性卡那霉素抗性弹夹(aphT)插入侧翼DNA片段之间以便取代鼠伤寒沙门氏菌tatC基因。然后将该DNA片段转入自杀载体pCVD442。接着通过将pCVD442构建体连入野生型鼠伤寒沙门氏菌菌株TML和SL1344后的等位转移使鼠伤寒沙门氏菌tatC基因的染色体拷贝发生突变。

在使用混合型和单一型感染的全身感染的小鼠模型体内对破坏的鼠伤寒沙门氏菌tatC基因测试毒力的减毒作用。对于混合型感染来说，给6-7周龄balbC小鼠经腹膜内接种104个细菌细胞。在比较3天后存在于脾中的突变体和野生型细菌的数量后来计算竞争指数。对于单一型感染来说，经腹膜内或口服给小鼠接种不同剂量并对小鼠的存活率监测17天。菌株的毒性得到弱化，SL1344 tatC和TML tatC缺失菌株的竞争指数分别为0.078和0.098。

在单一型感染中，小鼠的存活比野生型对照组小鼠的存活延长。

还通过来自脑膜炎奈瑟氏球菌的tat序列证明了序列同源性。将来自脑膜炎奈瑟氏球菌的基因序列表示为SEQ ID NO.20并将所编码的tatC的氨基酸序列表示为SEQ ID NO.21。

为了测试毒性，使用下列引物生成缺失突变体：

5’-TGCTCTAGACACATCATGGGCACACC-3’(SEQ ID NO.69)

5’-GAACTGCAGAACCGTCCACATCAGGCG-3’(SEQ ID NO.70)

5’-GAACTGCAGACCCTGCTTGCCATTCCG-3’(SEQ ID NO.71)

5’-GAACTGCAGACCCTGCTTGCCATTCCG-3’(SEQ ID NO.72)

在将DNA片段和aphT卡那霉素抗性弹夹克隆入pUC19后进行上述对鼠伤寒沙门氏菌所概括的步骤。通过将以pUC19为基础的构建体转化入野生型脑膜炎奈瑟氏球菌细胞而使脑膜炎奈瑟氏球菌tatC基因的染色体拷贝发生突变。

对所得转化体的DNA分析表明所有转化体均是部分二倍体且含有野生型和突变型tatC基因拷贝。这表明对tatC基因已经缺失的突变体的分离存在一定的选择作用。

对极性和非极性构建体的进一步研究证实转化体不在选择性培养基上生长。这提示脑膜炎奈瑟氏球菌tatC基因是该生物体体外生长所必需的。

实施例7

在本文鉴定为SEQ ID NO.22的核苷酸序列内的3981位核苷酸上根据插入的转座子来鉴定另外一种突变体。本文定义为eck1的序列显示出与来自许多细菌的几种1组糖基转移酶的序列同源性。还证实了与大肠杆菌K12的gnd基因(SEQ ID NO.22的4197-4604位核苷酸上)的序列同源性。

将翻译的大肠杆菌eck1基因表示为SEQ ID NO.26。已经如上所述测试了该基因的毒力减毒作用且证实该基因以0.025的竞争指数被减毒。

还根据DNA序列(SEQ ID NO.22)鉴定了几种可读框(ORF)。将它们中的第一种在本文中定义为MS1并将翻译产物表示为SEQ IDNO.25。证实其氨基酸序列与来自大肠杆菌血清型O111(TrEMBL数据库登记号AAD46732)的推定糖基转移酶具有50.3％的同一性。该氨基酸序列还显示出与来自大肠杆菌K1的eck1蛋白质的同源性以及与来自小肠结膜炎耶尔森氏菌(TrEMBL数据库登记号Q56917)的TrsE蛋白质的同源性。

本文中鉴定为MS2的第二种可读框具有表示为SEQ ID NO.24的基因序列。这证明了与来自小肠结膜炎耶尔森氏菌(TrEMBL数据库登记号Q56915)的推定糖基转移酶TrsC的序列同源性以及与来自大肠杆菌血清型O113(TrEMBL数据库登记号AAD50485)的糖基转移酶WbnA的序列同源性。

第三种可读框编码本文鉴定为MS3(SEQ ID NO.23)的产物。其氨基酸序列表现出与来自变异链球菌的鼠李糖基转移酶30.2％的同一性。

如果多个毒力基因成群定位于微生物的基因组上，那么表示为SEQID NO.22的基因序列可以是致病性岛的至少一部分。

实施例8

鉴定另外一种在iroCDE操纵子内带有插入的转座子的突变体。将位于小Tn5插入物侧翼的核苷酸序列表示为SEQ ID NO.27和SEQ IDNO.30。

将小Tn5转座子插在SEQ ID NO.27的1272位核苷酸上和SEQ IDNO.30的1位核苷酸上并打断iroD基因。将iroD的N-末端区表示为SEQID NO.29并将C-末端区表示为SEQ ID NO.31。

除iroD外，表示为SEQ ID NO.27的基因还编码带有表示为SEQ IDNO.28的氨基酸序列的部分肽。这种氨基酸序列表现出与来自伤寒沙门氏菌的推定ATP结合弹夹转运蛋白iroC 70.9％的同一性。

表示为SEQ ID NO.30的基因序列包括编码带有表示为SEQ IDNO.32的氨基酸序列的肽的可读框且它与来自伤寒沙门氏菌的iroE蛋白质具有序列同源性。

如上所述在毒力的减毒作用的模型中对基因测试表明以0.107的竞争指数对iroD基因进行了减毒。已经通过P1转导将iroD基因中的小Tn5突变重新引入野生型大肠杆菌K1菌株。还以0.1的竞争指数对所得转导体的毒力进行了减毒。这表明减毒表型与iroD内的插入物连接。然而，因对大肠杆菌K1 iroE基因的极性作用而导致减毒是可能的。

实施例9

用表示为SEQ ID NO.33的核苷酸序列内插入的转座子鉴定另外一种突变体。将该转座子插在SEQ ID NO.33的2264位核苷酸上。该核苷酸序列表现出与大肠杆菌K12(EMBL登记号AE000456)的aslA/hemY区的序列同源性。aslA编码芳基硫酸酯酶同源物，而hemY与血红素IX的生物合成有关。这证明受到破坏的区至少部分与大肠杆菌K12的aslA/hemY区相同。

将转座子插在SEQ ID NO.33的2264位核苷酸上。该插入位点是来自hemY基因的终止密码子下游的216个核苷酸和来自aslA基因的起始密码子上游的472个核苷酸。

如上所述对新区测试毒力的减毒作用且证实该新区以0.033的竞争指数被减毒。已经通过P1转导将该区中的小Tn5突变重新引入野生型大肠杆菌K1菌株。还以0.1的竞争指数对所得的转导体的毒力进行了减毒。这表明减毒表型与该区中的转座子插入物连接。不过，如上所述构建aslA的极性和非极性缺失突变体并测试其对毒力的减毒作用。

无论是极性还是非极性突变体的毒力均没有弱化且这证明原始转座子突变体的减毒作用并不是因对aslA基因的极性作用而导致的。这表明转座子正在破坏在aslA与hemY之间的基因间隔区内编码的某些其它功能。例如，可能存在某些在该区内编码未翻译的RNA分子诸如与oxyS相似的调节RNA(Altuvia等《细胞》(Cell)1997；90：43-53)。另一方面，转座子可能破坏例如与DNA复制相关的某些DNA结构。该DNA区还存在于病原体鼠伤寒沙门氏菌中，从而提示它对其它生物体中的致病性来说可能是重要的。可以将该区(SEQ ID NO.33)用作靶物以鉴定抗微生物药。

实施例10

鉴定另外一种突变体并克隆位于小Tn5插入物侧翼的DNA区且它具有表示为SEQ ID NO.34的核苷酸序列。由于mtd2基因产物起胞嘧啶特异性甲基转移酶的作用，所以该核苷酸序列与橙色滑柱菌(EMBL登记号P25265)的mtd2基因具有同源性。在大肠杆菌K12中没有发现mtd2基因且它可以代表致病性岛。

小Tn5转座子插入物定位于SEQ ID NO.34的4773和3764位核苷酸上且证实它可打断mtd2基因。

将mtd2基因的氨基酸序列表示为SEQ ID NO.43。

如上所述对大肠杆菌K1 mtd2基因测试对毒力的减毒作用且证实该基因以0.073的竞争指数被减毒。

除mtd2基因外，还使用本文分别鉴定为MS4-MS16、SEQ IDNOS.48-44和42-35的翻译产物鉴定了一系列可读框。如果可读框定位于潜在致病性岛中，那么这些基因中的突变也可以使毒力弱化。此外，由于已知大肠杆菌和其它细菌可以编码核苷酸序列中不同形式的肽类，所以这些蛋白质中的某些的编码区可以重叠。此外，显示出以Val开始的任意氨基酸序列实际上可以以Met开始。

序列表

<110>Microscience Limited

<120>毒力基因和蛋白质及其用途

<130>REP05921WO

<140>

<141>

<160>72

<170>PatentIn 2.1版

<210>1

<211>4333

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(1017)..(2549)

<400>1

ccattactca gaatgggcgg atacacaata aaaattgttc ttcttattac cgcataaccg 60

atgccgaggc acaaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta 120

tcagaatttt ctaaattatt tctgatacgt ttgaatatcc agacgcacag cgtcgtcatg 180

accactaaca ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat 240

ttgtcataca atgacagtcc aggccaactt tccgctttcc ctttgacgta ttgcagcata 300

ataaattgcg gcaatgtcag tagggggatg gctgttaaca tcgggatacc tacacgttcg 360

acacgtactt tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtac 420

ccggatataa cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtc 480

agccataatg acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa 540

atatgaaaag ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca 600

cgttgcgcgg gtactgggtt catatatggt taactaatct cggatttttc gtcttatccc 660

tgtcgggtta tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc 720

aggcttcaag gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg 780

taagacggtt gataaataaa tttgctggca aaccctacac gaagtcgatg cttctgtctt 840

taggagaagc acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagtgt 900

gctgaacgat accgggattc tgttgtcgga atggcaggtt atccattaaa atagatcgga 960

tcgatataag cacacaaagg gggaagtgct tactaattat gaaacataaa ctacaa atg 1019

Met

1

atg aaa atg cgt tgg ttg agt gct gca gta atg tta acc ctg tat aca 1067

Met Lys Met Arg Trp Leu Ser Ala Ala Val Met Leu Thr Leu Tyr Thr

5 10 15

tct tca agc tgg gct ttc agt att gat gat gtc gca aag caa gct caa 1115

Ser Ser Ser Trp Ala Phe Ser Ile Asp Asp Val Ala Lys Gln Ala Gln

20 25 30

tcc tta gcc ggg aaa ggc tat gag gcg ccc aaa agc aac ttg ccc tcc 1163

Ser Leu Ala Gly Lys Gly Tyr Glu Ala Pro Lys Ser Asn Leu Pro Ser

35 40 45

gtt ttc cgc gat atg aaa tac gcg gac tat cag cag atc cag ttt aat 1211

Val Phe Arg Asp Met Lys Tyr Ala Asp Tyr Gln Gln Ile Gln Phe Asn

50 55 60 65

cat gac aaa gcg tac tgg aac aat ctg aag acc cca ttc aaa ctc gag 1259

His Asp Lys Ala Tyr Trp Asn Asn Leu Lys Thr Pro Phe Lys Leu Glu

70 75 80

ttc tac cat cag ggt atg tac ttc gat acc ccg gtc aaa ata aat gaa 1307

Phe Tyr His Gln Gly Met Tyr Phe Asp Thr Pro Val Lys Ile Asn Glu

85 90 95

gtg act gcc acc gca gtc aaa cga atc aaa tac agc ccg gat tat ttc 1355

Val Thr Ala Thr Ala Val Lys Arg Ile Lys Tyr Ser Pro Asp Tyr Phe

100 105 110

act ttc ggc gat gtt cag cat gac aaa gac acg gta aaa gac ctt ggt 1403

Thr Phe Gly Asp Val Gln His Asp Lys Asp Thr Val Lys Asp Leu Gly

115 120 125

ttt gcc ggt ttc aaa gtg ctt tac ccg atc aac agc aaa gat aaa aac 1451

Phe Ala Gly Phe Lys Val Leu Tyr Pro Ile Asn Ser Lys Asp Lys Asn

130 135 140 145

gat gaa atc gtc agc atg ctc ggg gcc agc tat ttc cgc gtg att ggt 1499

Asp Glu Ile Val Ser Met Leu Gly Ala Ser Tyr Phe Arg Val Ile Gly

150 155 160

gca ggt cag gtt tat ggc ctt tct gca cgc ggc ctg gca att gat acc 1547

Ala Gly Gln Val Tyr Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp Thr

165 170 175

gcc ttg cca tcg ggt gaa gaa ttt cca cgc ttc aaa gag ttc tgg atc 1595

Ala Leu Pro Ser Gly Glu Glu Phe Pro Arg Phe Lys Glu Phe Trp Ile

180 185 190

gag cgt cca aaa ccg act gat aaa cgt tta acc att tat gca ttg ctt 1643

Glu Arg Pro Lys Pro Thr Asp Lys Arg Leu Thr Ile Tyr Ala Leu Leu

195 200 205

gac tcg ccg cgc gcg aca ggt gct tac aaa ttc gta gtt atg cca gga 1691

Asp Ser Pro Arg Ala Thr Gly Ala Tyr Lys Phe Val Val Met Pro Gly

2l0 215 220 225

cgt gac acg gtt gtg gat gtg cag tcg aaa atc tat ctg cgc gat aaa 1739

Arg Asp Thr Val Val Asp Val Gln Ser Lys Ile Tyr Leu Arg Asp Lys

230 235 240

gtc ggc aaa ctg ggg gtt gca ccg tta acc agt atg ttc ctg ttt ggg 1787

Val Gly Lys Leu Gly Val Ala Pro Leu Thr Ser Met Phe Leu Phe Gly

245 250 255

ccg aac caa ccg tcg cct gca aat aac tat cgt ccg gag ttg cac gac 1835

Pro Asn Gln Pro Ser Pro Ala Asn Asn Tyr Arg Pro Glu Leu His Asp

260 265 270

tct aac ggt ctg tct atc cat gct ggt aat ggc gaa tgg atc tgg cgt 1883

Ser Asn Gly Leu Ser Ile His Ala Gly Asn Gly Glu Trp Ile Trp Arg

275 280 285

ccg ttg aat aac ccg aaa cat tta gcg gtc agc agc ttc tcg atg gaa 1931

Pro Leu Asn Asn Pro Lys His Leu Ala Val Ser Ser Phe Ser Met Glu

290 295 300 305

aac ccg caa ggc ttc ggt cta ttg cag cgt ggt cgt gat ttc tcc cgc 1979

Asn Pro Gln Gly Phe Gly Leu Leu Gln Arg Gly Arg Asp Phe Ser Arg

310 315 320

ttt gaa gat ctc gat gat cgt tac gat ctt cgt cca agc gca tgg gtg 2027

Phe Glu Asp Leu Asp Asp Arg Tyr Asp Leu Arg Pro Ser Ala Trp Val

325 330 335

act ccg aaa ggg gag tgg ggc aaa ggc agc gtt gag ctg gtg gaa att 2075

Thr Pro Lys Gly Glu Trp Gly Lys Gly Ser Val Glu Leu Val Glu Ile

340 345 350

cca acc aac gat gaa acc aac gat aac atc gtc gct tac tgg acg ccg 2123

Pro Thr Asn Asp Glu Thr Asn Asp Asn Ile Val Ala Tyr Trp Thr Pro

355 360 365

gat cag ctg ccg gag ccg ggt aaa gag atg aac ttt aaa tac acc atc 2171

Asp Gln Leu Pro Glu Pro Gly Lys Glu Met Asn Phe Lys Tyr Thr Ile

370 375 380 385

acc ttc agc cgt gat gaa gac aaa ctg cat gcg cca gat aac gca tgg 2219

Thr Phe Ser Arg Asp Glu Asp Lys Leu His Ala Pro Asp Asn Ala Trp

390 395 400

gtg caa caa acg cgt cgt tca acg ggg gat gtg aag cag tcg aac ctg 2267

Val Gln Gln Thr Arg Arg Ser Thr Gly Asp Val Lys Gln Ser Asn Leu

405 410 415

att cgc cag cct gac ggt act atc gcc ttt gtg gtc gat ttt acc ggc 2315

Ile Arg Gln Pro Asp Gly Thr Ile Ala Phe Val Val Asp Phe Thr Gly

420 425 430

gct gag atg aaa aaa ctg cca gag gat acc ccg gtc aca gcg caa acc 2363

Ala Glu Met Lys Lys Leu Pro Glu Asp Thr Pro Val Thr Ala Gln Thr

435 440 445

agc att ggt gat aat ggt gag ata gtt gaa agc acg gtg cgt tat aac 2411

Ser Ile Gly Asp Asn Gly Glu Ile Val Glu Ser Thr Val Arg Tyr Asn

450 455 460 465

ccg gtt acc aaa ggc tgg cgt ctg gtg atg cgt gtg aaa gtg aaa gat 2459

Pro Val Thr Lys Gly Trp Arg Leu Val Met Arg Val Lys Val Lys Asp

470 475 480

gcc aag aaa acc act gaa atg cgt gct gcg ctg gtg aat gcc gat cag 2507

Ala Lys Lys Thr Thr Glu Met Arg Ala Ala Leu Val Asn Ala Asp Gln

485 490 495

acg ttg agt gaa acc tgg agc tac cag tta cct gcc aat gaa 2549

Thr Leu Ser Glu Thr Trp Ser Tyr Gln Leu Pro Ala Asn Glu

500 505 510

taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag cggcattgcc 2609

gaagactgat atccgcgccg ttcatcaggc gctggatgcc gaacaccgca cctgggcgcg 2669

ggaggatgac tccccgcaag gctcggtaaa ggcgcgtctg gaacaagcct ggccagattc 2729

acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctaa aggcgatgcc 2789

agaagtaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg taggccgttt 2849

ctgggatcgc ctgcgtggac gcgatgtgac gccgcgctat ctggctcgtt tgaccaaaga 2909

agagcaggag agtgagcaaa agtggcgtac cgtcggtacc atccgccgtt acattctgtt 2969

gatcctgacg ctcgcgcaaa ctgttgtcgc gacctggtat atgaagacca ttcttcctta 3029

tcaggggtgg gcgctgatta atcctatgga tatggttggt caggatgtgt gggtttcctt 3089

tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctctttg cggtactgtt 3149

ctgttgggtg tccgccggat tctggaccgg cgttgatggg cttcctgcaa ctgcttattg 3209

gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc 3269

atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct 3329

tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacatttt gatgtctaca 3389

ttcttagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc 3449

ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgc cgtcgcgtga 3509

agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca 3569

tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttgtgcggc ctggtgcgcc 3629

tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggcatgg 3689

atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag 3749

ccggtttgca cttctggcaa cttggcgagt cgcactactg ggggcataac gcgattatcc 3809

gcgtgaaacc gtttatcgag cactgtgcac tggctccgct gccgggcgaa ggttcttttg 3869

ccggttcaat cctgtcacat gacttcgtgg aagcggcgtt gatgcgccgt gcaggttggg 3929

gggtctggat tgcttacgat ctcccgggtt cttatgaaga attaccgcct aacttgcttg 3989

atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc 4049

tggtgaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc 4109

tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtacatg 4169

cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggta tggccgcagt 4229

ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga 4289

agctattgag cattttgctt atctggtgca aaggaacgaa agaa 4333

<210>2

<211>511

<212>PRT

<213>大肠杆菌

<400>2

Met Met Lys Met Arg Trp Leu Ser Ala Ala Val Met Leu Thr Leu Tyr

1 5 10 15

Thr Ser Ser Ser Trp Ala Phe Ser Ile Asp Asp Val Ala Lys Gln Ala

20 25 30

Gln Ser Leu Ala Gly Lys Gly Tyr Glu Ala Pro Lys Ser Asn Leu Pro

35 40 45

Ser Val Phe Arg Asp Met Lys Tyr Ala Asp Tyr Gln Gln Ile Gln Phe

50 55 60

Asn His Asp Lys Ala Tyr Trp Asn Asn Leu Lys Thr Pro Phe Lys Leu

65 70 75 80

Glu Phe Tyr His Gln Gly Met Tyr Phe Asp Thr Pro Val Lys Ile Asn

85 90 95

Glu Val Thr Ala Thr Ala Val Lys Arg Ile Lys Tyr Ser Pro Asp Tyr

100 105 110

Phe Thr Phe Gly Asp Val Gln His Asp Lys Asp Thr Val Lys Asp Leu

115 120 125

Gly Phe Ala Gly Phe Lys Val Leu Tyr Pro Ile Asn Ser Lys Asp Lys

130 135 140

Asn Asp Glu Ile Val Ser Met Leu Gly Ala Ser Tyr Phe Arg Val Ile

145 150 155 160

Gly Ala Gly Gln Val Tyr Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp

165 170 175

Thr Ala Leu Pro Ser Gly Glu Glu Phe Pro Arg Phe Lys Glu Phe Trp

180 185 190

Ile Glu Arg Pro Lys Pro Thr Asp Lys Arg Leu Thr Ile Tyr Ala Leu

195 200 205

Leu Asp Ser Pro Arg Ala Thr Gly Ala Tyr Lys Phe Val Val Met Pro

210 215 220

Gly Arg Asp Thr Val Val Asp Val Gln Ser Lys Ile Tyr Leu Arg Asp

225 230 235 240

Lys Val Gly Lys Leu Gly Val Ala Pro Leu Thr Ser Met Phe Leu Phe

245 250 255

Gly Pro Asn Gln Pro Ser Pro Ala Asn Asn Tyr Arg Pro Glu Leu His

260 265 270

Asp Ser Asn Gly Leu Ser Ile His Ala Gly Asn Gly Glu Trp Ile Trp

275 280 285

Arg Pro Leu Asn Asn Pro Lys His Leu Ala Val Ser Ser Phe Ser Met

290 295 300

Glu Asn Pro Gln Gly Phe Gly Leu Leu Gln Arg Gly Arg Asp Phe Ser

305 310 315 320

Arg Phe Glu Asp Leu Asp Asp Arg Tyr Asp Leu Arg Pro Ser Ala Trp

325 330 335

Val Thr Pro Lys Gly Glu Trp Gly Lys Gly Ser Val Glu Leu Val Glu

340 345 350

Ile Pro Thr Asn Asp Glu Thr Asn Asp Asn Ile Val Ala Tyr Trp Thr

355 360 365

Pro Asp Gln Leu Pro Glu Pro Gly Lys Glu Met Asn Phe Lys Tyr Thr

370 375 380

Ile Thr Phe Ser Arg Asp Glu Asp Lys Leu His Ala Pro Asp Asn Ala

385 390 395 400

Trp Val Gln Gln Thr Arg Arg Ser Thr Gly Asp Val Lys Gln Ser Asn

405 410 415

Leu Ile Arg Gln Pro Asp Gly Thr Ile Ala Phe Val Val Asp Phe Thr

420 425 430

Gly Ala Glu Met Lys Lys Leu Pro Glu Asp Thr Pro Val Thr Ala Gln

435 440 445

Thr Ser Ile Gly Asp Asn Gly Glu Ile Val Glu Ser Thr Val Arg Tyr

450 455 460

Asn Pro Val Thr Lys Gly Trp Arg Leu Val Met Arg Val Lys Val Lys

465 470 475 480

Asp Ala Lys Lys Thr Thr Glu Met Arg Ala Ala Leu Val Asn Ala Asp

485 490 495

Gln Thr Leu Ser Glu Thr Trp Ser Tyr Gln Leu Pro Ala Asn Glu

500 505 510

<210>3

<211>574

<212>DNA

<213>大肠杆菌

<400>3

ttcgttgatc ctgtcaccgt ttgttcggtt atttccagcc gtgccaccgt tggtctgcga 60

accaaacgct ggaaactgtt ccctgatccc ggaagagtat tcaccgccgc aggtgctggt 120

tgataccgat cggttccttg agatgaatcg tcaatgctcc cttgatgatg gttttatgca 180

cgcggtgttt aacccgtcat ttaacgctct ggcaaccgca atggcgaccg cgcgtcaccg 240

cgccagcaag gtgctggaaa tcgcccgtga ccgccacgtt gaacaggcgc tgaacgagac 300

gccagagaag ctgaatcgcg atcgtcgcct ggtgctgcta agcgatccgg tgacgatggc 360

ccgtctgcat ttccgcgtct ggaattcccc ggagagatat tcttcatggg tgagttatta 420

cgaagggata aagctcaatc cactggcatt gcgtaaaccg gatgcggctt cgcaataaaa 480

acgtagttgc ctgatgcgct acgcttatca ggcctacatc gttcctgcaa tttattgatt 540

ttgcaagatt ttgtaggtcg gataaggcgt tcac 574

<210>4

<211>1478

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(25)..(1449)

<400>4

gggataatgc ctgaggggcc tgta atg cgt atc ggc atg cgg ttg ttg ctg 51

Met Arg Ile Gly Met Arg Leu Leu Leu

1 5

ggc tat ttt tta ctg gtg gcg gtg gcg gcc tgg ttc gta ctg gct att 99

Gly Tyr Phe Leu Leu Val Ala Val Ala Ala Trp Phe Val Leu Ala Ile

10 15 20 25

ttt gtc aaa gaa gtt aaa ccg ggc gtg cga aga gca acc gag ggg acg 147

Phe Val Lys Glu Val Lys Pro Gly Val Arg Arg Ala Thr Glu Gly Thr

30 35 40

tta atc gac acc gca acg ttg ctg gcg gag ctg gcg cgt ccc gat ttg 195

Leu Ile Asp Thr Ala Thr Leu Leu Ala Glu Leu Ala Arg Pro Asp Leu

45 50 55

ctc tct ggg gac cca acg cat ggg caa ctg gcg cag gcg ttt aat cag 243

Leu Ser Gly Asp Pro Thr His Gly Gln Leu Ala Gln Ala Phe Asn Gln

60 65 70

cta caa cat cgc ccg ttt cgc gcc aat atc ggt ggc att aac aaa gtg 291

Leu Gln His Arg Pro Phe Arg Ala Asn Ile Gly Gly Ile Asn Lys Val

75 80 85

cgc aac gaa tat cat gtc tat atg acc gat gcg cag ggc aaa gta ttg 339

Arg Asn Glu Tyr His Val Tyr Met Thr Asp Ala Gln Gly Lys Val Leu

90 95 100 105

ttc gat tcg gca aat aaa gcc gtt gga cag gat tat tcg cgc tgg aat 387

Phe Asp Ser Ala Asn Lys Ala Val Gly Gln Asp Tyr Ser Arg Trp Asn

110 115 120

gac gtc tgg cta acg ttg cgt ggt cag tat ggt gcg cgc agc acg ttg 435

Asp Val Trp Leu Thr Leu Arg Gly Gln Tyr Gly Ala Arg Ser Thr Leu

125 130 135

caa aat cct gcc gat ccc gaa agt tct gtg atg tat gtt gcc gcg ccg 483

Gln Asn Pro Ala Asp Pro Glu Ser Ser Val Met Tyr Val Ala Ala Pro

140 145 150

att atg gac ggc tcg cgg ctt att ggc gtt ttg agc gta ggc aaa ccg 531

Ile Met Asp Gly Ser Arg Leu Ile Gly Val Leu Ser Val Gly Lys Pro

155 160 165

aac gcg gcg atg gct ccg gtc att aag cgt agc gag cgg cga att tta 579

Asn Ala Ala Met Ala Pro Val Ile Lys Arg Ser Glu Arg Arg Ile Leu

170 175 180 185

tgg gcc agc gcc att ttg ttg ggg att gca ctg gtg att ggc gca ggc 627

Trp Ala Ser Ala Ile Leu Leu Gly Ile Ala Leu Val Ile Gly Ala Gly

190 195 200

atg gtt tgg tgg atc aac cgc tct att gcc agg ctc act cgc tat gct 675

Met Val Trp Trp Ile Asn Arg Ser Ile Ala Arg Leu Thr Arg Tyr Ala

205 210 215

gat tcc gtc act gac aat aag ccc gtt cct ctc ccc gat ctc ggt agt 723

Asp Ser Val Thr Asp Asn Lys Pro Val Pro Leu Pro Asp Leu Gly Ser

220 225 230

agc gag ttg cgt aaa ctc gcg cag gcg ctg gaa agt atg cgc gtg aag 771

Ser Glu Leu Arg Lys Leu Ala Gln Ala Leu Glu Ser Met Arg Val Lys

235 240 245

ctg gaa ggg aaa aac tat att gag cag tat gtt tat gcg tta act cat 819

Leu Glu Gly Lys Asn Tyr Ile Glu Gln Tyr Val Tyr Ala Leu Thr His

250 255 260 265

gag cta aaa agc cca ctg gcg gcg att cgt ggc gcg gcg gaa att tta 867

Glu Leu Lys Ser Pro Leu Ala Ala Ile Arg Gly Ala Ala Glu Ile Leu

270 275 280

cgc gaa ggt ccg ccg ccg gaa gtg gtg gct cgt ttt acc gac aac att 915

Arg Glu Gly Pro Pro Pro Glu Val Val Ala Arg Phe Thr Asp Asn Ile

285 290 295

ctg acg caa aat gcg cga atg cag gca ctg gtg gaa acg tta cta cgc 963

Leu Thr Gln Asn Ala Arg Met Gln Ala Leu Val Glu Thr Leu Leu Arg

300 305 310

cag gca aga ctg gag aat cgt cag gaa gtc gtt ctg act gct gtt gat 1011

Gln Ala Arg Leu Glu Asn Arg Gln Glu Val Val Leu Thr Ala Val Asp

315 320 325

gtg gcg gca tta ttt cgc cgc gtc agc gaa gcg cgc acc gtg cag ttg 1059

Val Ala Ala Leu Phe Arg Arg Val Ser Glu Ala Arg Thr Val Gln Leu

330 335 340 345

gca gaa aaa aac atc act ttg cat gtt atg cct act gag gtt aac gtt 1107

Ala Glu Lys Asn Ile Thr Leu His Val Met Pro Thr Glu Val Asn Val

350 355 360

gct tct gaa ccg gcg tta ctg gag cag gcg ctg ggg aat tta ctg gat 1155

Ala Ser Glu Pro Ala Leu Leu Glu Gln Ala Leu Gly Asn Leu Leu Asp

365 370 375

aac gcc atc gat ttt act ccc gag agc ggt tgc ata acg cta agc gcc 1203

Asn Ala Ile Asp Phe Thr Pro Glu Ser Gly Cys Ile Thr Leu Ser Ala

380 385 390

gaa gtg gat cag gaa tac gtc acc ctt aag gtg ctg gat acc ggt agt 1251

Glu Val Asp Gln Glu Tyr Val Thr Leu Lys Val Leu Asp Thr Gly Ser

395 400 405

ggg att cct gac tac gcg ctg tca cgt att ttt gaa cgc ttt tac tct 1299

Gly Ile Pro Asp Tyr Ala Leu Ser Arg Ile Phe Glu Arg Phe Tyr Ser

410 415 420 425

ttg ccg cgt gca aat ggg caa aaa agc agc ggt ctg ggg ttg gcg ttt 1347

Leu Pro Arg Ala Asn Gly Gln Lys Ser Ser Gly Leu Gly Leu Ala Phe

430 435 440

gtc agt gag gtc gcc cgt ttg ttt aac ggc gaa gtc acg ctg cgc aac 1395

Val Ser Glu Val Ala Arg Leu Phe Asn Gly Glu Val Thr Leu Arg Asn

445 450 455

gtg cag gaa ggt ggc gtg ctg gcc tcg ctt cga ctt cac cgt cac ttc 1443

Val Gln Glu Gly Gly Val Leu Ala Set Leu Arg Leu His Arg His Phe

460 465 470

aca tag cttcaaattc ttcccacata gtcttcgta 1478

Thr

475

<210>5

<211>474

<212>PRT

<213>大肠杆菌

<400>5

Met Arg Ile Gly Met Arg Leu Leu Leu Gly Tyr Phe Leu Leu Val Ala

1 5 10 15

Val Ala Ala Trp Phe Val Leu Ala Ile Phe Val Lys Glu Val Lys Pro

20 25 30

Gly Val Arg Arg Ala Thr Glu Gly Thr Leu Ile Asp Thr Ala Thr Leu

35 40 45

Leu Ala Glu Leu Ala Arg Pro Asp Leu Leu Ser Gly Asp Pro Thr His

50 55 60

Gly Gln Leu Ala Gln Ala Phe Asn Gln Leu Gln His Arg Pro Phe Arg

65 70 75 80

Ala Asn Ile Gly Gly Ile Asn Lys Val Arg Asn Glu Tyr His Val Tyr

85 90 95

Met Thr Asp Ala Gln Gly Lys Val Leu Phe Asp Ser Ala Asn Lys Ala

100 105 110

Val Gly Gln Asp Tyr Ser Arg Trp Asn Asp Val Trp Leu Thr Leu Arg

115 120 125

Gly Gln Tyr Gly Ala Arg Ser Thr Leu Gln Asn Pro Ala Asp Pro Glu

130 135 140

Ser Ser Val Met Tyr Val Ala Ala Pro Ile Met Asp Gly Ser Arg Leu

145 150 155 160

Ile Gly Val Leu Ser Val Gly Lys Pro Asn Ala Ala Met Ala Pro Val

165 170 175

Ile Lys Arg Ser Glu Arg Arg Ile Leu Trp Ala Ser Ala Ile Leu Leu

180 185 190

Gly Ile Ala Leu Val Ile Gly Ala Gly Met Val Trp Trp Ile Asn Arg

195 200 205

Ser Ile Ala Arg Leu Thr Arg Tyr Ala Asp Ser Val Thr Asp Asn Lys

210 215 220

Pro Val Pro Leu Pro Asp Leu Gly Ser Ser Glu Leu Arg Lys Leu Ala

225 230 235 240

Gln Ala Leu Glu Ser Met Arg Val Lys Leu Glu Gly Lys Asn Tyr Ile

245 250 255

Glu Gln Tyr Val Tyr Ala Leu Thr His Glu Leu Lys Ser Pro Leu Ala

260 265 270

Ala Ile Arg Gly Ala Ala Glu Ile Leu Arg Glu Gly Pro Pro Pro Glu

275 280 285

Val Val Ala Arg Phe Thr Asp Asn Ile Leu Thr Gln Asn Ala Arg Met

290 295 300

Gln Ala Leu Val Glu Thr Leu Leu Arg Gln Ala Arg Leu Glu Asn Arg

305 310 315 320

Gln Glu Val Val Leu Thr Ala Val Asp Val Ala Ala Leu Phe Arg Arg

325 330 335

Val Ser Glu Ala Arg Thr Val Gln Leu Ala Glu Lys Asn Ile Thr Leu

340 345 350

His Val Met Pro Thr Glu Val Asn Val Ala Ser Glu Pro Ala Leu Leu

355 360 365

Glu Gln Ala Leu Gly Asn Leu Leu Asp Asn Ala Ile Asp Phe Thr Pro

370 375 380

Glu Ser Gly Cys Ile Thr Leu Ser Ala Glu Val Asp Gln Glu Tyr Val

385 390 395 400

Thr Leu Lys Val Leu Asp Thr Gly Ser Gly Ile Pro Asp Tyr Ala Leu

405 410 415

Ser Arg Ile Phe Glu Arg Phe Tyr Ser Leu Pro Arg Ala Asn Gly Gln

420 425 430

Lys Ser Ser Gly Leu Gly Leu Ala Phe Val Ser Glu Val Ala Arg Leu

435 440 445

Phe Asn Gly Glu Val Thr Leu Arg Asn Val Gln Glu Gly Gly Val Leu

450 455 460

Ala Ser Leu Arg Leu His Arg His Phe Thr

465 470

<210>6

<211>128

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(1)..(126)

<400>6

atg aaa ggt cgc ctg tta gat gct gtc ccg ctc agt tcc cta acg ggc 48

Met Lys Gly Arg Leu Leu Asp Ala Val Pro Leu Ser Ser Leu Thr Gly

1 5 10 15

gtt ggc gca gcg ctt agt aac aag ctg gcg aaa atc aac ctg cat acc 96

Val Gly Ala Ala Leu Ser Asn Lys Leu Ala Lys Ile Asn Leu His Thr

20 25 30

gta cag gat tta ctc tta cac ctt cct ctg cg 128

Val Gln Asp Leu Leu Leu His Leu Pro Leu

35 40

<210>7

<211>42

<212>PRT

<213>大肠杆菌

<400>7

Met Lys Gly Arg Leu Leu Asp Ala Val Pro Leu Ser Ser Leu Thr Gly

1 5 10 15

Val Gly Ala Ala Leu Ser Asn Lys Leu Ala Lys Ile Asn Leu His Thr

20 25 30

Val Gln Asp Leu Leu Leu His Leu Pro Leu

35 40

<210>8

<211>1174

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(121)..(837)

<400>8

agatgcacga tcgagtaggc cggataaggc gtttacgccg catccagcat ggaaaacgcg 60

cactttgtta tcaatctggg gccagcaaat gctggcctga tttgttcttg agggaagact 120

atg atg cgc aaa atg ctg ctg gcg gca gca ctt tca gtg acg gca atg 168

Met Met Arg Lys Met Leu Leu Ala Ala Ala Leu Ser Val Thr Ala Met

1 5 10 15

acc gct cac gcc gac tac cag tgc agc gtc acg ccg cgt gac gat gtg 216

Thr Ala His Ala Asp Tyr Gln Cys Ser Val Thr Pro Arg Asp Asp Val

20 25 30

att gtc agc ccg caa acc gtg cag gtg aag ggc gaa aac ggc aat ctg 264

Ile Val Ser Pro Gln Thr Val Gln Val Lys Gly Glu Asn Gly Asn Leu

35 40 45

gtg atc acg cca gac ggc aac gtg atg tat aac ggt aag caa tat tcc 312

Val Ile Thr Pro Asp Gly Asn Val Met Tyr Asn Gly Lys Gln Tyr Ser

50 55 60

ctg aat gcc gcc cag cgc gag cag gcg aag gat tat cag gct gaa cta 360

Leu Asn Ala Ala Gln Arg Glu Gln Ala Lys Asp Tyr Gln Ala Glu Leu

65 70 75 80

cgt agc acc ctg ccg tgg att gat gga ggc gcg aaa agc cgc gtc gag 408

Arg Ser Thr Leu Pro Trp Ile Asp Gly Gly Ala Lys Ser Arg Val Glu

85 90 95

aaa gct cgt att gcg ctg gat aaa att atc gtt cag gag atg ggc gaa 456

Lys Ala Arg Ile Ala Leu Asp Lys Ile Ile Val Gln Glu Met Gly Glu

100 105 110

agc agc aaa atg cgc agc cgt ctg acc aaa ctt gat gcg cag ctg aaa 504

Ser Ser Lys Met Arg Ser Arg Leu Thr Lys Leu Asp Ala Gln Leu Lys

115 120 125

gag cag atg aac cgc att atc gaa acg cgc agc gat ggc ctg acg ttt 552

Glu Gln Met Asn Arg Ile Ile Glu Thr Arg Ser Asp Gly Leu Thr Phe

130 135 140

cac tat aaa gcc att gat cag gtt cgt gcc gaa ggc cag caa tta gtg 600

His Tyr Lys Ala Ile Asp Gln Val Arg Ala Glu Gly Gln Gln Leu Val

145 150 155 160

aat cag gca atg ggc gga att tta cag gac agc att aat gaa atg ggc 648

Asn Gln Ala Met Gly Gly Ile Leu Gln Asp Ser Ile Asn Glu Met Gly

165 170 175

gcg aaa gcg gtg ctg aaa agc ggc ggt aac cca tta cag aac gtg ctg 696

Ala Lys Ala Val Leu Lys Ser Gly Gly Asn Pro Leu Gln Asn Val Leu

180 185 190

gga agc ctg ggc ggc ctg caa tcc tca atc caa acc gag tgg aaa aag 744

Gly Ser Leu Gly Gly Leu Gln Ser Ser Ile Gln Thr Glu Trp Lys Lys

195 200 205

cag gaa aaa gat ttc cag cag ttt ggc aaa gat gtt tgt agc cgc gtt 792

Gln Glu Lys Asp Phe Gln Gln Phe Gly Lys Asp Val Cys Ser Arg Val

210 215 220

gtg act ctg gaa gat agc cgc aaa gcc ctg gtc ggg aat tta aaa 837

Val Thr Leu Glu Asp Ser Arg Lys Ala Leu Val Gly Asn Leu Lys

225 230 235

taatcctcta ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt 897

acctgcctct aactttgtaa gggcgaattc tgcagatatc catcacactg gcggccgctc 957

gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc 1017

gtcgttttac aaccgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 1077

agcacatccc cctttcgcca gctggcgtaa tagcgaaaag gcccgcaccg atcgcccttc 1137

caacagttgc gcacctgatg gccaatggac gcgcctg 1174

<210>9

<211>239

<212>PRT

<213>大肠杆菌

<400>9

Met Met Arg Lys Met Leu Leu Ala Ala Ala Leu Ser Val Thr Ala Met

1 5 10 15

Thr Ala His Ala Asp Tyr Gln Cys Ser Val Thr Pro Arg Asp Asp Val

20 25 30

Ile Val Ser Pro Gln Thr Val Gln Val Lys Gly Glu Asn Gly Asn Leu

35 40 45

Val Ile Thr Pro Asp Gly Asn Val Met Tyr Asn Gly Lys Gln Tyr Ser

50 55 60

Leu Asn Ala Ala Gln Arg Glu Gln Ala Lys Asp Tyr Gln Ala Glu Leu

65 70 75 80

Arg Ser Thr Leu Pro Trp Ile Asp Gly Gly Ala Lys Ser Arg Val Glu

85 90 95

Lys Ala Arg Ile Ala Leu Asp Lys Ile Ile Val Gln Glu Met Gly Glu

100 105 110

Ser Ser Lys Met Arg Ser Arg Leu Thr Lys Leu Asp Ala Gln Leu Lys

115 120 125

Glu Gln Met Asn Arg Ile Ile Glu Thr Arg Ser Asp Gly Leu Thr Phe

130 135 140

His Tyr Lys Ala Ile Asp Gln Val Arg Ala Glu Gly Gln Gln Leu Val

145 150 155 160

Asn Gln Ala Met Gly Gly Ile Leu Gln Asp Ser Ile Asn Glu Met Gly

165 170 175

Ala Lys Ala Val Leu Lys Ser Gly Gly Asn Pro Leu Gln Asn Val Leu

180 185 190

Gly Ser Leu Gly Gly Leu Gln Ser Ser Ile Gln Thr Glu Trp Lys Lys

195 200 205

Gln Glu Lys Asp Phe Gln Gln Phe Gly Lys Asp Val Cys Ser Arg Val

210 215 220

Val Thr Leu Glu Asp Ser Arg Lys Ala Leu Val Gly Asn Leu Lys

225 230 235

<210>10

<211>3406

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(1007)..(1276)

<220>

<221>CDS

<222>(1280)..(1792)

<220>

<221>CDS

<222>(1798)..(2574)

<220>

<221>CDS

<222>(2604)..(3398)

<400>10

gatgatggtg atggagcgta tttacggcat tccggtgtct gatgttgcga cgctggagaa 60

aaacggcacc aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt 120

ctttcgcgac agctttttcc atgctgatat gcaccctggc aacatcttcg taagctatga 180

acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa 240

agaagataaa cgctatctgg cggaaaactt tatcgccttc tttaatcgcg actatcgcaa 300

agtggcagag ctacacgtcg attctggttg ggtgccacca gataccaacg ttgaagagtt 360

cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc 420

gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca 480

gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct 540

ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc ctggagtcgt ggattaaaga 600

tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga 660

aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca 720

gcatagtgtt ggtaagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatt 780

cgcgttattt tctcggaatt ggcgctacgt tagtatttaa gtggcacatt cttgttggtc 840

agccgacctg aatgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg 900

tttgtccggt tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg 960

tataatgcgg ctttgtttaa tcatcatcta ccacagagga acatgt atg ggt ggt 1015

Met Gly Gly

1

atc agt att tgg cag tta ttg att att gcc gtc atc gtt gta ctg ctt 1063

Ile Ser Ile Trp Gln Leu Leu Ile Ile Ala Val Ile Val Val Leu Leu

5 10 15

ttt ggc acc aaa aag ctc ggc tcc atc ggt tcc gat ctt ggt gcg tcg 1111

Phe Gly Thr Lys Lys Leu Gly Ser Ile Gly Ser Asp Leu Gly Ala Ser

20 25 30 35

atc aaa ggc ttt aaa aaa gca atg agc gat gat gaa cca aag cag gat 1159

Ile Lys Gly Phe Lys Lys Ala Met Ser Asp Asp Glu Pro Lys Gln Asp

40 45 50

aaa acc agc cag gat gct gat ttt act gcg aaa act atc gcc gat aag 1207

Lys Thr Ser Gln Asp Ala Asp Phe Thr Ala Lys Thr Ile Ala Asp Lys

55 60 65

cag gcg gat acg aat cag gaa cag gct aaa ata gaa gac gcg aag cgc 1255

Gln Ala Asp Thr Asn Gln Glu Gln Ala Lys Ile Glu Asp Ala Lys Arg

70 75 80

cac gat aaa gag cag gtg taa tct gtg ttt gat atc ggt ttt agc gaa 1303

His Asp Lys Glu Gln Val Val Phe Asp Ile Gly Phe Ser Glu

85 90 95

ctg cta ttg gtg ttc atc atc ggc ctc gtc gtt ctg ggg ccg caa cga 1351

Leu Leu Leu Val Phe Ile Ile Gly Leu Val Val Leu Gly Pro Gln Arg

100 105 110

ctg cct gtg gcg gta aaa acg gta gcg ggc tgg att cgc gcg ttg cgt 1399

Leu Pro Val Ala Val Lys Thr Val Ala Gly Trp Ile Arg Ala Leu Arg

115 120 125 130

tca ctg gcg aca acg gtg cag aac gaa ctg acc cag gag tta aaa ctc 1447

Ser Leu Ala Thr Thr Val Gln Asn Glu Leu Thr Gln Glu Leu Lys Leu

135 140 145

cag gag ttt cag gac agt ctg aaa aag gtt gaa aag gcg agc ctc act 1495

Gln Glu Phe Gln Asp Ser Leu Lys Lys Val Glu Lys Ala Ser Leu Thr

150 155 160

aac ctg acg ccc gaa ctg aaa gcg tcg atg gat gaa tta cgc cag gct 1543

Asn Leu Thr Pro Glu Leu Lys Ala Ser Met Asp Glu Leu Arg Gln Ala

165 170 175

gcg gag tcg atg aaa cgt tcc tac gtt 9ca aac gat cct gaa aag gcg 1591

Ala Glu Ser Met Lys Arg Ser Tyr Val Ala Asn Asp Pro Glu Lys Ala

180 185 190

agc gat gaa gcg cac acc atc cat aac ccg gtg gtg aaa gag aat gaa 1639

Ser Asp Glu Ala His Thr Ile His Asn Pro Val Val Lys Asp Asn Glu

195 200 205 210

act gcg cat gaa ggc gta acg cct gct gct gca caa acg cag gcc agt 1687

Thr Ala His Glu Gly Val Thr Pro Ala Ala Ala Gln Thr Gln Ala Ser

215 220 225

tcg ccg gaa cag aag cca gaa acc acg cca gag ccg gtg gta aaa cct 1735

Ser Pro Glu Gln Lys Pro Glu Thr Thr Pro Glu Pro Val Val Lys Pro

230 235 240

gct gcg gac gct gaa ccg aaa acc gct gca cct tcc cct tcg tcg agt 1783

Ala Ala Asp Ala Glu Pro Lys Thr Ala Ala Pro Ser Pro Ser Ser Ser

245 250 255

gat aaa ccg taaac atg tct gta gaa gat act caa ccg ctt atc acg cat 1833

Asp Lys Pro Met Ser Val Glu Asp Thr Gln Pro Leu Ile Thr His

260 265 270

ctg att gag ctg cgt aag cgt ctg ctg aac tgc att atc tcg gtg atc 1881

Leu Ile Glu Leu Arg Lys Arg Leu Leu Asn Cys Ile Ile Ser Val Ile

275 280 285

gtg ata ttc ctg tgt ctg gtc tat ttc gcc aat gac atc tat cac ctg 1929

Val Ile Phe Leu Cys Leu Val Tyr Phe Ala Asn Asp Ile Tyr His Leu

290 295 300 305

gta tcc gcg cca ctg atc aag cag ttg ccg caa ggt tca acg atg atc 1977

Val Ser Ala Pro Leu Ile Lys Gln Leu Pro Gln Gly Ser Thr Met Ile

310 315 320

gcc acc gac gtg gcc tcg ccg ttc ttt acg ccg atc aag ctg acc ttt 2025

Ala Thr Asp Val Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe

325 330 335

atg gtg tcg ctg att ctg tca gcg ccg gtg att ctc tat cag gtg tgg 2073

Met Val Ser Leu Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp

340 345 350

gcg ttt atc gcc cca gcg ctg tat aag cat gaa cgt cgc ctg gtg gtg 2121

Ala Phe Ile Ala Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val

355 360 365

ccg ctg ctg gtt tcc agc tct ctg ctg ttt tat atc ggc atg gcg ttc 2169

Pro Leu Leu Val Ser Set Set Leu Leu Phe Tyr Ile Gly Met Ala Phe

370 375 380 385

gcc tac ttt gtg gtc ttt ccg ctg gca ttt ggc ttc ctt gcc aat acc 2217

Ala Tyr Phe Val Val Phe Pro Leu Ala Phe Gly Phe Leu Ala Asn Thr

390 395 400

gcg ccg gaa ggg gta cag gta tcc acc gac atc gcg agc tat tta agc 2265

Ala Pro Glu Gly Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser

405 410 415

ttc gtt atg gcg ctg ttt atg gcg ttt ggt gtc tcc ttt gaa gtg ccg 2313

Phe Val Met Ala Leu Phe Met Ala Phe Gly Val Ser Phe Glu Val Pro

420 425 430

gtg gca att gtg ctg ctg tgc tgg atg ggg att acc tcg cca gaa gac 2361

Val Ala Ile Val Leu Leu Cys Trp Met Gly Ile Thr Ser Pro Glu Asp

435 440 445

tta cgc aaa aaa cgc ccg tat gtg ctg gtt ggt gca ttc gtt gtc ggg 2409

Leu Arg Lys Lys Arg Pro Tyr Val Leu Val Gly Ala Phe Val Val Gly

450 455 460 465

atg ttg ctg acg ccg ccg gat gtc ttc tcg caa acg ctg ttg gcg atc 2457

Met Leu Leu Thr Pro Pro Asp Val Phe Ser Gln Thr Leu Leu Ala Ile

470 475 480

cct atg tac tgc ctg ttt gaa atc ggt gtc ttc ttc tca cgc ttt tac 2505

Pro Met Tyr Cys Leu Phe Glu Ile Gly Val Phe Phe Ser Arg Phe Tyr

485 490 495

gtt ggt aaa ggg cga aac cgg gaa gag gaa aac gac gct gaa gca gaa 2553

Val Gly Lys Gly Arg Asn Arg Glu Glu Glu Asn Asp Ala Glu Ala Glu

500 505 510

agc gaa aaa act gaa gaa taa attcaaccgc ccgtcagggc ggttgtcat atg 2606

Ser Glu Lys Thr Glu Glu Met

515 520

gag tac agg atg ttt gat atc ggc gtt aat ttg acc agt tcg caa ttt 2654

Glu Tyr Arg Met Phe Asp Ile Gly Val Asn Leu Thr Ser Ser Gln Phe

525 530 535

gcg aaa gac cgt gat gat gtt gta gcg cgc gct ttt gac gcg gga gtt 2702

Ala Lys Asp Arg Asp Asp Val Val Ala Arg Ala Phe Asp Ala Gly Val

540 545 550

aat ggg cta ctc atc acc ggt acc aat ctg cgt gaa agc cag cag gcg 2750

Asn Gly Leu Leu Ile Thr Gly Thr Asn Leu Arg Glu Ser Gln Gln Ala

555 560 565

caa aag ctg gcg cgt cag tat tcg tcc tgt tgg tca acg gcg ggc gta 2798

Gln Lys Leu Ala Arg Gln Tyr Ser Ser Cys Trp Ser Thr Ala Gly Val

570 575 580 585

cat cct cac gac agc agc cag tgg caa gct gtg act gaa gaa gcg att 2846

His Pro His Asp Ser Ser Gln Trp Gln Ala Val Thr Glu Glu Ala Ile

590 595 600

att gag ctg gcc gcg cag cca gaa gtg gtg gcg att ggt gaa tgt ggt 2894

Ile Glu Leu Ala Ala Gln Pro Glu Val Val Ala Ile Gly Glu Cys Gly

605 610 615

ctc gac ttt aac cgc aac ttt tcg acg ccg gaa gag cag gaa cgc gct 2942

Leu Asp Phe Asn Arg Asn Phe Ser Thr Pro Glu Glu Gln Glu Arg Ala

620 625 630

ttt gtt gcc cag cta cgc att gcc gca gaa tta aac atg ccg gta ttt 2990

Phe Val Ala Gln Leu Arg Ile Ala Ala Glu Leu Asn Met Pro Val Phe

635 640 645

atg cac tgt cgc gat gcc cac gag cgg ttt atg aca ttg ctg gag ccg 3038

Met His Cys Arg Asp Ala His Glu Arg Phe Met Thr Leu Leu Glu Pro

650 655 660 665

tgg ctg gat aaa ctg cct ggt gcg gtt ctt cat tgc ttt acc ggc aca 3086

Trp Leu Asp Lys Leu Pro Gly Ala Val Leu His Cys Phe Thr Gly Thr

670 675 680

cgc gaa gag atg cag gcg tgc gtg gcg tgt gga att tat atc ggc att 3134

Arg Glu Glu Met Gln Ala Cys Val Ala Cys Gly Ile Tyr Ile Gly Ile

685 690 695

acc ggt tgg gtt tgc gat gaa cga cgc ggg ctg gag ctg cgg gaa ttg 3182

Thr Gly Trp Val Cys Asp Glu Arg Arg Gly Leu Glu Leu Arg Glu Leu

700 705 710

ttg ccg ttg att ccg gcg gag aaa ttg ctg atc gaa act gat gcg ccg 3230

Leu Pro Leu Ile Pro Ala Glu Lys Leu Leu Ile Glu Thr Asp Ala Pro

715 720 725

tat ctg ctc cct cgc gat ctc acg cca aag cca tca tcc cgg cgc aac 3278

Tyr Leu Leu Pro Arg Asp Leu Thr Pro Lys Pro Ser Ser Arg Arg Asn

730 735 740 745

gag cca gcc cat ctg ccc cat att ttg caa cgt att gcg cac tgg cgt 3326

Glu Pro Ala His Leu Pro His Ile Leu Gln Arg Ile Ala His Trp Arg

750 755 760

gga gaa gat gcc gca tgg ctg gct gcc acc acg gat gcc aat gtc aaa 3374

Gly Glu Asp Ala Ala Trp Leu Ala Ala Thr Thr Asp Ala Asn Val Lys

765 770 775

aca ctg ttt ggg att gcg ttt tag agtttgcg 3406

Thr Leu Phe Gly Ile Ala Phe

780 785

<210>11

<211>89

<212>PRT

<213>大肠杆菌

<400>11

Met Gly Gly Ile Ser Ile Trp Gln Leu Leu Ile Ile Ala Val Ile Val

1 5 10 15

Val Leu Leu Phe Gly Thr Lys Lys Leu Gly Ser Ile Gly Ser Asp Leu

20 25 30

Gly Ala Ser Ile Lys Gly Phe Lys Lys Ala Met Ser Asp Asp Glu Pro

35 40 45

Lys Gln Asp Lys Thr Ser Gln Asp Ala Asp Phe Thr Ala Lys Thr Ile

50 55 60

Ala Asp Lys Gln Ala Asp Thr Asn Gln Glu Gln Ala Lys Ile Glu Asp

65 70 75 80

Ala Lys Arg His Asp Lys Glu Gln Val

85

<210>12

<211>171

<212>PRT

<213>大肠杆菌

<400>12

Val Phe Asp Ile Gly Phe Ser Glu Leu Leu Leu Val Phe Ile Ile Gly

1 5 10 15

Leu Val Val Leu Gly Pro Gln Arg Leu Pro Val Ala Val Lys Thr Val

20 25 30

Ala Gly Trp Ile Arg Ala Leu Arg Ser Leu Ala Thr Thr Val Gln Asn

35 40 45

Glu Leu Thr Gln Glu Leu Lys Leu Gln Glu Phe Gln Asp Ser Leu Lys

50 55 60

Lys Val Glu Lys Ala Ser Leu Thr Asn Leu Thr Pro Glu Leu Lys Ala

65 70 75 80

Ser Met Asp Glu Leu Arg Gln Ala Ala Glu Ser Met Lys Arg Ser Tyr

85 90 95

Val Ala Asn Asp Pro Glu Lys Ala Ser Asp Glu Ala His Thr Ile His

100 105 110

Asn Pro Val Val Lys Asp Asn Glu Thr Ala His Glu Gly Val Thr Pro

115 120 125

Ala Ala Ala Gln Thr Gln Ala Ser Ser Pro Glu Gln Lys Pro Glu Thr

130 135 140

Thr Pro Glu Pro Val Val Lys Pro Ala Ala Asp Ala Glu Pro Lys Thr

145 150 155 160

Ala Ala Pro Ser Pro Ser Ser Ser Asp Lys Pro

165 170

<210>13

<211>258

<212>PRT

<213>大肠杆菌

<400>13

Met Ser Val Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu

1 5 10 15

Arg Lys Arg Leu Leu Asn Cys Ile Ile Ser Val Ile Val Ile Phe Leu

20 25 30

Cys Leu Val Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ser Ala Pro

35 40 45

Leu Ile Lys Gln Leu Pro Gln Gly Ser Thr Met Ile Ala Thr Asp Val

50 55 60

Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu

65 70 75 80

Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala

85 90 95

Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val

100 105 110

Ser Ser Ser Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val

115 120 125

Val Phe Pro Leu Ala Phe Gly Phe Leu Ala Asn Thr Ala Pro Glu Gly

130 135 140

Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala

145 150 155 160

Leu Phe Met Ala Phe Gly Val Ser Phe Glu Val Pro Val Ala Ile Val

165 170 175

Leu Leu Cys Trp Met Gly Ile Thr Ser Pro Glu Asp Leu Arg Lys Lys

180 185 190

Arg Pro Tyr Val Leu Val Gly Ala Phe Val Val Gly Met Leu Leu Thr

195 200 205

Pro Pro Asp Val Phe Ser Gln Thr Leu Leu Ala Ile Pro Met Tyr Cys

210 215 220

Leu Phe Glu Ile Gly Val Phe Phe Ser Arg Phe Tyr Val Gly Lys Gly

225 230 235 240

Arg Asn Arg Glu Glu Glu Asn Asp Ala Glu Ala Glu Ser Glu Lys Thr

245 250 255

Glu Glu

<210>14

<211>264

<212>PRT

<213>大肠杆菌

<400>14

Met Glu Tyr Arg Met Phe Asp Ile Gly Val Asn Leu Thr Ser Ser Gln

1 5 10 15

Phe Ala Lys Asp Arg Asp Asp Val Val Ala Arg Ala Phe Asp Ala Gly

20 25 30

Val Asn Gly Leu Leu Ile Thr Gly Thr Asn Leu Arg Glu Ser Gln Gln

35 40 45

Ala Gln Lys Leu Ala Arg Gln Tyr Ser Ser Cys Trp Ser Thr Ala Gly

50 55 60

Val His Pro His Asp Ser Ser Gln Trp Gln Ala Val Thr Glu Glu Ala

65 70 75 80

Ile Ile Glu Leu Ala Ala Gln Pro Glu Val Val Ala Ile Gly Glu Cys

85 90 95

Gly Leu Asp Phe Asn Arg Asn Phe Ser Thr Pro Glu Glu Gln Glu Arg

100 105 110

Ala Phe Val Ala Gln Leu Arg Ile Ala Ala Glu Leu Asn Met Pro Val

115 120 125

Phe Met His Cys Arg Asp Ala His Glu Arg Phe Met Thr Leu Leu Glu

130 135 140

Pro Trp Leu Asp Lys Leu Pro Gly Ala Val Leu His Cys Phe Thr Gly

145 150 155 160

Thr Arg Glu Glu Met Gln Ala Cys Val Ala Cys Gly Ile Tyr Ile Gly

165 170 175

Ile Thr Gly Trp Val Cys Asp Glu Arg Arg Gly Leu Glu Leu Arg Glu

180 185 190

Leu Leu Pro Leu Ile Pro Ala Glu Lys Leu Leu Ile Glu Thr Asp Ala

195 200 205

Pro Tyr Leu Leu Pro Arg Asp Leu Thr Pro Lys Pro Ser Ser Arg Arg

210 215 220

Asn Glu Pro Ala His Leu Pro His Ile Leu Gln Arg Ile Ala His Trp

225 230 235 240

Arg Gly Glu Asp Ala Ala Trp Leu Ala Ala Thr Thr Asp Ala Asn Val

245 250 255

Lys Thr Leu Phe Gly Ile Ala Phe

260

<210>15

<211>586

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(170)..(370)

<400>15

tcttaaacaa ccgtcgcttt gcgccgccgc aattattatg atgttttttt actcggcgct 60

tgattcacct tgttacagat tgctattgtg tgcgcgcgtc gaatgaccgt taatattctc 120

tggtttttaa ggcgcgttct gttgccggtt atatgtcaag aaggtatct atg ggt gag 178

Met Gly Glu

1

att agt att acc aaa ctg ctg gta gtt gcg gcg ctg gtc gtt ctg ctg 226

Ile Ser Ile Thr Lys Leu Leu Val Val Ala Ala Leu Val Val Leu Leu

5 10 15

ttt ggg act aag aag tta cgt acg ctg ggc gga gac ctt gga gcg gcc 274

Phe Gly Thr Lys Lys Leu Arg Thr Leu Gly Gly Asp Leu Gly Ala Ala

20 25 30 35

att aaa ggg ttc aag aag gcg atg aat gat gac gat gct gcg gcg aaa 322

Ile Lys Gly Phe Lys Lys Ala Met Asn Asp Asp Asp Ala Ala Ala Lys

40 45 50

aaa ggc gca gac gtt gat ctt cag gct gaa aag ctc tct cat aaa gag 370

Lys Gly Ala Asp Val Asp Leu Gln Ala Glu Lys Leu Set His Lys Glu

55 60 65

tgacgtggcg agcaggacgc tccctcaata tcttgttcga tacaaaaacc cgcttcaaaa 430

agcgggtttt ttatcagaca gatgtaagta attattacag gattacttaa cttccatccc 490

tttcgcctgc aaatcggcgt ggtaagaaga gcggacaaac ggaccgcatg cagcatgggt 550

aaagcccatc gccagcgctt cgctttcatt tcgtcg 586

<210>16

<211>67

<212>PRT

<213>大肠杆菌

<400>16

Met Gly Glu Ile Ser Ile Thr Lys Leu Leu Val Val Ala Ala Leu Val

1 5 10 15

Val Leu Leu Phe Gly Thr Lys Lys Leu Arg Thr Leu Gly Gly Asp Leu

20 25 30

Gly Ala Ala Ile Lys Gly Phe Lys Lys Ala Met Asn Asp Asp Asp Ala

35 40 45

Ala Ala Lys Lys Gly Ala Asp Val Asp Leu Gln Ala Glu Lys Leu Ser

50 55 60

His Lys Glu

65

<210>17

<211>4200

<212>DNA

<213>鼠伤寒沙门氏菌

<220>

<221>CDS

<222>(947)..(1444)

<220>

<221>CDS

<222>(1450)..(1722)

<400>17

cgcaagtcaa tgtcgtcccg gtcgtatgta aaagtatgtg aatagggcgg gcgaaagcgg 60

ctaacaaaga ggcagcgtga aggataatgt gtataatgcg gccctaataa ttcatcatct 120

atcacagagg aacatgtatg ggtggtatca gtatttggca gttgttgatt gttgccgtta 180

tcgtcgtact gctgttcggc accaaaaaac tcggttccat cggttccgat cttggcgcgt 240

ctatcaaagg ctttaaaaag gccatgagcg atgatgatgc caaacaggat aaaaccagtc 300

aggacgctga ttttaccgct aaatctatcg cggataagca aggcgaagcg aaaaaggaag 360

acgctaaaag ccaagataaa gagcaggtat aatccgtgtt tgatatcggt tttagcgaac 420

tgctgttagt gttcgttatc ggcctcattg tgttggggcc gcaacgattg ccagtagcgg 480

taaaaacggt agcgggctgg attcgcgcgt tgcggtccct tgcgacaacg gttcagaatg 540

aactgactca ggaactgaaa cttcaggagt tccaggacag tctgaaaaaa gtcgaaaagg 600

cgagcctgga aaatctgact cccgaactga aagcatctat ggatgaactg cgtcaggcgg 660

cggagtcgat gaaacgcacc tacagcgcta acgatcccga acaagcgagc gatgaagcgc 720

ataccatcca taatccggtg gtaaaaggga acgaaacgca gcatgagggc gtcacccctg 780

ccgccgctga aacacaggcg agcgcgccgg aacaaaagcc ggagcccgtt aaagctaacg 840

tgcctgagtc gacggaaacc gcttccgtag ccacgataga cgccgagaag aaatccgctg 900

cgcctgttgt cgaatcttcc ccctcgtcga gtgataaacc gtaaac atg gct gta 955

Met Ala Val

1

gaa gat act caa ccg ctt atc acg cat ctg atc gag ttg cgt aag cgc 1003

Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu Arg Lys Arg

5 10 15

ctg cta aac tgc atc gtc gca gta crt ctg att ttt ctg gcg tta att 1051

Leu Leu Asn Cys Ile Val Ala Val Leu Leu Ile Phe Leu Ala Leu Ile

20 25 30 35

tat ttc gcc aat gat att tat cat tta gtc gcc gca ccg ctg att aaa 1099

Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ala Ala Pro Leu Ile Lys

40 45 50

cag atg ccg caa ggg gcg aca atg att gcg acg gat gtg gcg tcg ccg 1147

Gln Met Pro Gln Gly Ala Thr Met Ile Ala Thr Asp Val Ala Ser Pro

55 60 65

ttt ttt acg cct atc aaa ctc acc ttc atg gtg tct ttg atc tta tcc 1195

Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu Ile Leu Ser

70 75 80

gcg cct gtc att ttg tac cag gtt tgg gcc ttt atc gcc ccg gcg ctg 1243

Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala Pro Ala Leu

85 90 95

tat aag cat gag cgt cgt ctg gtc gta cct ctg ctg gta tcc agc tcg 1291

Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val Set Set Set

100 105 110 115

ctg ctt ttc tat att ggt atg gcc ttc gcc tat ttt gtc gta ttc cct 1339

Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val Val Phe Pro

120 125 130

ttg gcc ttt ggt ttc ctg acg cat acg gcg ccg gaa ggg gta cag gtt 1387

Leu Ala Phe Gly Phe Leu Thr His Thr Ala Pro Glu Gly Val Gln Val

135 140 145

tcg aca gat atc gcc agc tat ctt agc ttt gtc atg gcg ctt ttt atg 1435

Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala Leu Phe Met

150 155 160

gcc ttt gcg tagcc ttt gaa gtg ccg gtg gcg att gtg ttg ctg tgc tgg 1485

Ala Phe Ala Phe Glu Val Pro Val Ala Ile Val Leu Leu Cys Trp

165 170 175

atg ggc atc acc acg cca gaa gat ttg cgt aaa aaa cgg cct tat atc 1533

Met Gly Ile Thr Thr Pro Glu Asp Leu Arg Lys Lys Arg Pro Tyr Ile

180 185 190

ctg gtc ggg gca ttc att gtg gga atg ctg crt acg ccg cca gat gtt 1581

Leu Val Gly Ala Phe Ile Val Gly Met Leu Leu Thr Pro Pro Asp Val

195 200 205 210

ttc tcg caa acg ttg ctg gcg ata ccg atg tac tgc ctg ttt gaa att 1629

Phe Ser Gln Thr Leu Leu Ala Ile Pro Met Tyr Cys Leu Phe Glu Ile

215 220 225

ggc gtt ttc tgc tca cgc ttt tat gtc ggt aag cga cgg acg cgc gac 1677

Gly Val Phe Cys Ser Arg Phe Tyr Val Gly Lys Arg Arg Thr Arg Asp

230 235 240

gaa gat aac gag gcc gaa acc gaa aag gcc gag cac act gaa gac 1722

Glu Asp Asn Glu Ala Glu Thr Glu Lys Ala Glu His Thr Glu Asp

245 250 255

taaacacaac cgcccgccag ggcggttgtc atatgggggc aagcatgttt gatattggcg 1782

ttaatttaac cagtagccag tttgcaaaag atcgtgatga tgtggtcgcc cgtgcgtttg 1842

cggcgggagt aaaaggtatg ctactgaccg gaacgaacat ccatgaaagt cagcaggcgt 1902

taaaactggc gcggcgctac ccccattgtt ggtcgacggc tggcgtccat ccccatgaca 1962

gcagtcagtg gtcacccgcg tctgaagacg ccattattgc gctggcgaac cagccggaag 2022

tcgtcgctat cggtgagtgc gggctggatt tcaatcgcaa tttttccacg ccgcaggagc 2082

aggagcgtgc ctttcaggcg cagctacaaa ttgccgccga attgcagata ccaatcttta 2142

tgcactgccg ggacgcgcat gagcgatttc tggtattgct tgatccctgg ctggatagtc 2202

ttcctggtgc aatactgcac tgctttaccg gttcacgcca gcaaatgcag gcctgtgtgg 2262

atagagggct ctatatcggt attaccgggt gggtttgcga cgaacgacgc gggcttgagc 2322

tacgtgaact cttaccgttt attccagcgg aaaagctact gatagaaacc gacgcgccgt 2382

atctgttgcc tcgcgatctt acgccgaaac caacgtcacg acgcaacgag cccgcgtatc 2442

tgcctcacat cctggagcgc atagcgctat ggcgtggtga agatccgcaa tggttagcgg 2502

cgatgacaga tgccaacgcc agaaccttat ttgaggttgt attctgaacg atcgctaaat 2562

cttgcgaaaa ccggtgtttt ttacgctctg cttcacttct ttattgagta aattaagcag 2622

taacatcgaa cgcgtttcgc catccggttc ggtaaaaatc gctttcagcc cttcaaatgc 2682

gccttccgtg atgatgacgc tatcgccggg atagggggtt tcaggatcga caacgccttc 2742

gggcttgtag atagaaagct gatgaataac gctggaaggc acgatcgcag gatgccgcca 2802

aagcgcacaa aatggctgac gccgcgcgtg gcgttgattg tagtggtatg tatcacttcc 2862

ggatcaaatt caacgaacag ataattagga aagagcggtt cgctgacgga ggtacgtttt 2922

ccgcgtacca ttttttccag ggtgatcatc ggtgtcaggc aacttaccgc ttgtctttcg 2982

aggtgttcct gagcacgctg aagttgcccg cgtttgcagt acagtaaata ccaggattgc 3042

ataatgactc ttatccgctt gttcggggcg caagcatagc aaaagccatg cgcgaagtta 3102

attatacact tcatccttta agccgtatct ggattagcgt tggttgccag agttcacgct 3162

aatttaacaa aaatacagca tcccgatgat gaacgccgta taatgatgcg cttaccaaga 3222

ggctacaatg gacgccatga aatatcacga tttacgcgac ttcctgacgc tacttgagca 3282

gcagggggaa ctaaaacgca tcacgctacc tgtggatcct catctggaaa tcacggaaat 3342

cgctgaccgc acgctgcgtg ccggtggacc ggcgttgctg tttgaaaatc ctaaaggtta 3402

cgccatgccg gtgctgtgca acctttttgg cacgccaaaa cgcgtggcga tgggcatggg 3462

gcaggatgat gtttccgcct tacgggaagt gggtaaatta ttagcgtttc ttaaagaacc 3522

tgagccgccg aaagcgtttc gcgatctgtt tgacaagctg ccgcagttta agcaagtgct 3582

gaatatgccg acgaaacggt tacgcggcgc gccttgccag cagaaaatcg cgtctggcga 3642

tgatgtcgat ttaacgcgtc ttcctgtcat gacctgttgg ccggacgacg ccgcgccgct 3702

gattacctgg ggactgacgg taacgcgtgg tccgcacaaa gagcggcaaa acctgggcat 3762

ttatcgtcag cagttgatag gtaaaaataa gctgattatg cgctggctgt ctcaccgcgg 3822

cggcgcgctg gattttcagg agtggttagc cgcgcgtccg ggtgaacgtt tcccggtctc 3882

cgtcgcattg ggcgccgatc cggcacgata cttggcgccg tgactcctgt tcccgatact 3942

ctgtcggagt atgcctttgc gggcctgctg cgcggcacga aaactgaagt ggttaatgct 4002

ttctacgatc tggagtgctg cagcgcgaga tatcttgaag tacatgagcg gagagatgcg 4062

cggagacgta tgcgatcata cggcatatat gagtgatagc tcgtcttacg tcacgcaata 4122

acagcgtaga tgcatctata tcactatacg cgcgcatgag ctcgtatagg tgcctcatat 4182

ctcgtctatc tcaaagtc 4200

<210>18

<211>166

<212>PRT

<213>鼠伤寒沙门氏菌

<400>18

Met Ala Val Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu

1 5 10 15

Arg Lys Arg Leu Leu Asn Cys Ile Val Ala Val Leu Leu Ile Phe Leu

20 25 30

Ala Leu Ile Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ala Ala Pro

35 40 45

Leu Ile Lys Gln Met Pro Gln Gly Ala Thr Met Ile Ala Thr Asp Val

50 55 60

Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu

65 70 75 80

Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala

85 90 95

Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val

100 105 110

Ser Ser Ser Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val

115 120 125

Val Phe Pro Leu Ala Phe Gly Phe Leu Thr His Thr Ala Pro Glu Gly

130 135 140

Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala

145 150 155 160

Leu Phe Met Ala Phe Ala

165

<210>19

<211>91

<212>PRT

<213>鼠伤寒沙门氏菌

<400>19

Phe Glu Val Pro Val Ala Ile Val Leu Leu Cys Trp Met Gly Ile Thr

1 5 10 15

Thr Pro Glu Asp Leu Arg Lys Lys Arg Pro Tyr Ile Leu Val Gly Ala

20 25 30

Phe Ile Val Gly Met Leu Leu Thr Pro Pro Asp Val Phe Ser Gln Thr

35 40 45

Leu Leu Ala Ile Pro Met Tyr Cys Leu Phe Glu Ile Gly Val Phe Cys

50 55 60

Ser Arg Phe Tyr Val Gly Lys Arg Arg Thr Arg Asp Glu Asp Asn Glu

65 70 75 80

Ala Glu Thr Glu Lys Ala Glu His Thr Glu Asp

85 90

<210>20

<211>2601

<212>DNA

<213>脑膜炎奈瑟氏球菌

<220>

<221>CDS

<222>(1572)..(2339)

<400>20

agacaaaatc ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga 60

caaaaacccg tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct 120

tctgacacac cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgccaagg 180

tttgtcgggc ttggccgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg 240

cactttccca cattcaatcc gtctgaccgc tgttcagacg gcatcggagc cgttatggac 300

aactgtattt tctgcaaaat cgccgccaaa gacattccgg cgcaaaccgt ctatgaagac 360

ggcgaaatgg tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt 420

cccaaagtcc atttcgattc gttggcacac gccgcgcccg aacatcagcc ccttttggga 480

aaaatgatgc tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa 540

accctgatca acaccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc 600

atgggcacac ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata 660

catcatgggc agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat 720

attcggcacc aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt 780

caaacagggg ctgaacgaag gtacagacgg caaagaagcc caaaaagacg atgtaatcga 840

acacaaaaaa gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt 900

ttttgtcggc attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg 960

caccgccgga cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt 1020

tgacacgcaa atcgaactgg aagaactaag gaaggcaaag caggaatttg aagctgccgc 1080

tgctcaggtt cgagacagcc tcaaagaaac cggtacggat atggagggta atctgcacga 1140

catttccgac ggtctgaagc cttgggaaaa actgcccgaa cagcgcacgc ctgctgattt 1200

cggtgtcgat gaaaacggca atccctttcc cgatgcggca aacaccctat tagacggcat 1260

ttccgacgtt atgccgtccg aacgttccta cgcttccgcc gaaacccttg gggacagcgg 1320

gcaaaccggc agtacagccg aacccgcgga aaccgaccaa gaccgtgcat ggcgggaata 1380

cctgactgct tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac 1440

cgctgttgaa acccctgttc cgcataccac ttcgctgcgt aaacaggcaa taagccgcaa 1500

acgcgatttg cgtcctaaat cccgcgccaa acctaaattg cgcgtccgta aatcataaag 1560

agggcaatcc g gtg tcc gaa aca caa aac gaa caa ccc gtc caa ccg ctt 1610

Val Ser Glu Thr Gln Asn Glu Gln Pro Val Gln Pro Leu

1 5 10

gtc gag cat ctc atc gag ctg cgc cgc cgc ctg atg tgg acg gtt gtc 1658

Val Glu His Leu Ile Glu Leu Arg Arg Arg Leu Met Trp Thr Val Val

15 20 25

ggt atc tta gtc tgc ttt ttc ggc cta atg ccg ttt gcc caa caa ctc 1706

Gly Ile Leu Val Cys Phe Phe Gly Leu Met Pro Phe Ala Gln Gln Leu

30 35 40 45

tat act ttt atc gcc gac ccg ctg atg gca aac ctg ccc aaa gac acc 1754

Tyr Thr Phe Ile Ala Asp Pro Leu Met Ala Asn Leu Pro Lys Asp Thr

50 55 60

agc atg att gcc acc gat gtc atc gca cca ttt ttc gtg ccg gtc aaa 1802

Ser Met Ile Ala Thr Asp Val Ile Ala Pro Phe Phe Val Pro Val Lys

65 70 75

gtt acc ctg atg gcg gca ttt tta att tcg ctg ccg cat acg ctc tac 1850

Val Thr Leu Met Ala Ala Phe Leu Ile Ser Leu Pro His Thr Leu Tyr

80 85 90

caa atc tgg gca ttc gtc gcc ccc gca ctc tac caa aac gaa aaa cgc 1898

Gln Ile Trp Ala Phe Val Ala Pro Ala Leu Tyr Gln Asn Glu Lys Arg

95 100 105

ctg att acg ccg ctc gtc ctc tcc agc gtc agc ctg ttt ttc atc ggc 1946

Leu Ile Thr Pro Leu Val Leu Ser Ser Val Ser Leu Phe Phe Ile Gly

110 115 120 125

atg gca ttt gcc tac ttt ttg gtt ttc ccc gtc att ttc aaa ttc ctt 1994

Met Ala Phe Ala Tyr Phe Leu Val Phe Pro Val Ile Phe Lys Phe Leu

130 135 140

gcc agc gtt acc cct gtc ggt gtc aat atg gcg aca gac arc gac aaa 2042

Ala Sar Val Thr Pro Val Gly Val Asn Met Ala Thr Asp Ile Asp Lys

145 150 155

tac ctc tcc ttc atc ttg ggg atg ttt gtc gca ttc ggt aca acg ttt 2090

Tyr Leu Ser Phe Ile Leu Gly Met Phe Val Ala Phe Gly Thr Thr Phe

160 165 170

gaa gtc ccc att gtc gtt atc ctg tta acc aaa att ggt gtg gta aca 2138

Glu Val Pro Ile Val Val Ile Leu Leu Thr Lys Ile Gly Val Val Thr

175 180 185

acc gaa cag ctc aaa cgc gcc cgc ccc tat gtg att gtc ggc gcg ttt 2186

Thr Glu Gln Leu Lys Arg Ala Arg Pro Tyr Val Ile Val Gly Ala Phe

190 195 200 205

gtc att gcc gcc atc atc acg ccg ccc gat gtg att tca caa acc ctg 2234

Val Ile Ala Ala Ile Ile Thr Pro Pro Asp Val Ile Ser Gln Thr Leu

210 215 220

ctt gcc att ccg ctg att ctc tta tac gaa gca ggt att tgg ttc gga 2282

Leu Ala Ile Pro Leu Ile Leu Leu Tyr Glu Ala Gly Ile Trp Phe Gly

225 230 235

cgc ttt ttc acg cca cgt tca gaa cag gat ggc gac ata cag ccg cct 2330

Arg Phe Phe Thr Pro Arg Ser Glu Gln Asp Gly Asp Ile Gln Pro Pro

240 245 250

gca aca acc tgacactatg ccgtccgaac ctccgcctca taccgccaca 2379

Ala Thr Thr

255

gattaaggaa tacctttgaa taccctctat ttaggttcaa acagcccgcg ccgaatggaa 2439

atcctgacac agttgggcta tcaggtcgtc aagctgcctg ccaacatcga cgaaacggtc 2499

agacagaacg aagaccctgc ccgttacgtt caaaggatgg cagaagaaaa aaaccgaacc 2559

gccctgaccc tcttttgcga aaccaacggc acaatgcccg at 2601

<210>21

<211>256

<212>PRT

<213>脑膜炎奈瑟氏球菌

<400>21

Val Ser Glu Thr Gln Asn Glu Gln Pro Val Gln Pro Leu Val Glu His

1 5 10 15

Leu Ile Glu Leu Arg Arg Arg Leu Met Trp Thr Val Val Gly Ile Leu

20 25 30

Val Cys Phe Phe Gly Leu Met Pro Phe Ala Gln Gln Leu Tyr Thr Phe

35 40 45

Ile Ala Asp Pro Leu Met Ala Asn Leu Pro Lys Asp Thr Ser Met Ile

50 55 60

Ala Thr Asp Val Ile Ala Pro Phe Phe Val Pro Val Lys Val Thr Leu

65 70 75 80

Met Ala Ala Phe Leu Ile Ser Leu Pro His Thr Leu Tyr Gln Ile Trp

85 90 95

Ala Phe Val Ala Pro Ala Leu Tyr Gln Asn Glu Lys Arg Leu Ile Thr

100 105 110

Pro Leu Val Leu Ser Ser Val Ser Leu Phe Phe Ile Gly Met Ala Phe

115 120 125

Ala Tyr Phe Leu Val Phe Pro Val Ile Phe Lys Phe Leu Ala Ser Val

130 135 140

Thr Pro Val Gly Val Asn Met Ala Thr Asp Ile Asp Lys Tyr Leu Ser

145 150 155 160

Phe Ile Leu Gly Met Phe Val Ala Phe Gly Thr Thr Phe Glu Val Pro

165 170 175

Ile Val Val Ile Leu Leu Thr Lys Ile Gly Val Val Thr Thr Glu Gln

180 185 190

Leu Lys Arg Ala Arg Pro Tyr Val Ile Val Gly Ala Phe Val Ile Ala

195 200 205

Ala Ile Ile Thr Pro Pro Asp Val Ile Ser Gln Thr Leu Leu Ala Ile

210 215 220

Pro Leu Ile Leu Leu Tyr Glu Ala Gly Ile Trp Phe Gly Arg Phe Phe

225 230 235 240

Thr Pro Arg Ser Glu Gln Asp Gly Asp Ile Gln Pro Pro Ala Thr Thr

245 250 255

<210>22

<211>4604

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(2982)..(4082)

<220>

<221>CDS

<222>(1534)..(2637)

<220>

<221>CDS

<222>(749)..(1531)

<220>

<221>CDS

<222>(6)..(746)

<400>22

ggcta gtt gat gat aat ttg aaa ggt caa ggt gca gga aaa aat ttt tta 50

Val Asp Asp Asn Leu Lys Gly Gln Gly Ala Gly Lys Asn Phe Leu

1 5 10 15

tcg ctg ata aag tac agc gag aca gat tat aca att tat tgt gac caa 98

ser Leu Ile Lys Tyr Ser Glu Thr Asp Tyr Thr Ile Tyr Cys Asp Gln

20 25 30

gat gat att tgg tta gaa aac aaa ata ttt gaa tta gta aag tat gca 146

Asp Asp Ile Trp Leu Glu Asn Lys Ile Phe Glu Leu Val Lys Tyr Ala

35 40 45

aat gaa att aaa ttg aat gta tca gat gcg cct tcg cta gtt tat gct 194

Asn Glu Ile Lys Leu Asn Val Ser Asp Ala Pro Ser Leu Val Tyr Ala

50 55 60

gat ggc tat gct tat atg gat ggt gag ggt aca atc gat ttt tct ggg 242

Asp Gly Tyr Ala Tyr Met Asp Gly Glu Gly Thr Ile Asp Phe Ser Gly

65 70 75

ata tct aac aat cat gct gat caa tta aag gat ttt ctt ttt ttt aat 290

Ile Ser Asn Asn His Ala Asp Gln Leu Lys Asp Phe Leu Phe Phe Asn

80 85 90 95

ggt gga tac caa gga tgt tct att atg ttc aat cgt gca atg acc aaa 338

Gly Gly Tyr Gln Gly Cys Ser Ile Met Phe Asn Arg Ala Met Thr Lys

100 105 110

ttt ctt ctg aat tat cga gga ttt gta tat cta cat gac gat atc aca 386

Phe Leu Leu Asn Tyr Arg Gly Phe Val Tyr Leu His Asp Asp Ile Thr

115 120 125

aca tta gct gca tac gct ctt ggt aaa gtt tat ttt ctc ccg aaa tac 434

Thr Leu Ala Ala Tyr Ala Leu Gly Lys Val Tyr Phe Leu Pro Lys Tyr

130 135 140

ctt atg tta tat aga cag cac acg aat gcg gta act ggt atc aaa aca 482

Leu Met Leu Tyr Arg Gln His Thr Asn Ala Val Thr Gly Ile Lys Thr

145 150 155

ttc cgc aat gga ttg act tct aaa ttt aaa tca cca gta aac tat ctt 530

Phe Arg Asn Gly Leu Thr Ser Lys Phe Lys Ser Pro Val Asn Tyr Leu

160 165 170 175

tta tca cga aaa cat tat cag gta aaa aaa tct ttt ttt gaa tgt aac 578

Leu Ser Arg Lys His Tyr Gln Val Lys Lys Ser Phe Phe Glu Cys Asn

180 185 190

agc tct atc tta tca gag acg aat aaa aaa gtt ttt ttg gat ttt att 626

Ser Ser Ile Leu Ser Glu Thr Asn Lys Lys Val Phe Leu Asp Phe Ile

195 200 205

tca ttt tgt gaa tca aat aat aaa ttt aca gat ttt ttt aag tta tgg 674

Ser Phe Cys Glu Ser Asn Asn Lys Phe Thr Asp Phe Phe Lys Leu Trp

210 215 220

cga ggt ggg ttt aga tta aat aac agt aga act aaa tta tta tta aaa 722

Arg Gly Gly Phe Arg Leu Asn Asn Ser Arg Thr Lys Leu Leu Leu Lys

225 230 235

ttc tta ata cgg aga aaa ttt agc ga atg att tca ata ctt aca cct 769

Phe Leu Ile Arg Arg Lys Phe Ser Met Ile Ser Ile Leu Thr Pro

240 245 250

act ttt aat cgg caa cat act tta tca agg cta ttc aat tct ctt ata 817

Thr Phe Asn Arg Gln His Thr Leu Ser Arg Leu Phe Asn Ser Leu Ile

255 260 265 270

tta caa act gat aaa gat ttt gag tgg ata ata att gat gat ggt agt 865

Leu Gln Thr Asp Lys Asp Phe Glu Trp Ile Ile Ile Asp Asp Gly Set

275 280 285

ata gat gca aca gcg gta ctt gta gaa gat ttt aga aaa aaa tgt gat 913

Ile Asp Ala Thr Ala Val Leu Val Glu Asp Phe Arg Lys Lys Cys Asp

290 295 300

ttt gac ttg att tat tgc tat cag gaa aat aat ggt aag ccc atg gct 961

Phe Asp Leu Ile Tyr Cys Tyr Gln Glu Asn Asn Gly Lys Pro Met Ala

305 310 315

tta aac gct ggt gtt aaa gct tgt aga ggc gat tat atc ttt att gtt 1009

Leu Asn Ala Gly Val Lys Ala Cys Arg Gly Asp Tyr Ile Phe Ile Val

320 325 330

gac agt gat gat gca cta act ccc gat gcc ata aaa tta att aaa gaa 1057

Asp Ser Asp Asp Ala Leu Thr Pro Asp Ala Ile Lys Leu Ile Lys Glu

335 340 345 350

tca ata cat gat tgc tta tct gag aag gaa agt ttc agc gga gtc ggt 1105

Ser Ile His Asp Cys Leu Ser Glu Lys Glu Ser Phe Ser Gly Val Gly

355 360 365

ttt aga aaa gca tat ata aaa ggg ggg att att ggt aat gat tta aat 1153

Phe Arg Lys Ala Tyr Ile Lys Gly Gly Ile Ile Gly Asn Asp Leu Asn

370 375 380

aat tct tca gaa cat ata tac tat tta aat gcg act gag att agc aat 1201

Asn Ser Ser Glu His Ile Tyr Tyr Leu Asn Ala Thr Glu Ile Ser Asn

385 390 395

tta ata aat ggt gat gtt gca tat tgt ttt aaa aaa gaa agt ttg gta 1249

Leu Ile Asn Gly Asp Val Ala Tyr Cys Phe Lys Lys Glu Ser Leu Val

400 405 410

aaa aat cca ttc ccc cgt ata gaa gat gaa aaa ttt gtt cca gaa tta 1297

Lys Asn Pro Phe Pro Arg Ile Glu Asp Glu Lys Phe Val Pro Glu Leu

415 420 425 430

tat att tgg aat aaa ata act gac aag gcg aag att cga ttt aac ata 1345

Tyr Ile Trp Asn Lys Ile Thr Asp Lys Ala Lys Ile Arg Phe Asn Ile

435 440 445

agc aaa gtt ata tat ctt tgt gag tat ctt gat gat ggt ctt tct aaa 1393

Ser Lys Val Ile Tyr Leu Cys Glu Tyr Leu Asp Asp Gly Leu Ser Lys

450 455 460

aat tte cat aac cag ctt aaa aaa tac cca aag ggg ttt aag att tat 1441

Ash Phe His Asn Gln Leu Lys Lys Tyr Pro Lys Gly Phe Lys Ile Tyr

465 470 475

tac aaa gat caa aga aaa cga gag aaa act tat ata aaa aaa aca aag 1489

Tyr Lys Asp Gln Arg Lys Arg Glu Lys Thr Tyr Ile Lys Lys Thr Lys

480 485 490

atg cta att aga tat ttg caa tgt tgt tat tat gag aaa ata aa atg 1536

Met Leu Ile Arg Tyr Leu Gln Cys Cys Tyr Tyr Glu Lys Ile Met

495 500 505

aaa ata cta ttt gtc att aca ggt tta ggc ctt gga ggt gct gag aag 1584

Lys Ile Leu Phe Val Ile Thr Gly Leu Gly Leu Gly Gly Ala Glu Lys

510 515 520 525

cag gtt tgt ctt tta gct gat aaa tta agt tta agc ggg cac cat gta 1632

Gln Val Cys Leu Leu Ala Asp Lys Leu Ser Leu Ser Gly His His Val

530 535 540

aag att att tca ctt gga cat atg tct aat aat aaa gtc ttt cct agc 1680

Lys Ile Ile Ser Leu Gly His Met Ser Asn Asn Lys Val Phe Pro Ser

545 550 555

gaa aat aat gtt aat gtc att aat gta aat atg tca aaa aac att tct 1728

Glu Asn Asn Val Asn Val Ile Asn Val Asn Met Ser Lys Asn Ile Ser

560 565 570

gga gtt ata aaa ggt tgt gtc aga att aga gat gtt ata gct aat ttc 1776

Gly Val Ile Lys Gly Cys Val Arg Ile Arg Asp Val Ile Ala Asn Phe

575 580 585

aaa cca gac att gta cac agt cat atg ttt cat gca aac att atc act 1824

Lys Pro Asp Ile Val His Ser His Met Phe His Ala Asn Ile Ile Thr

590 595 600 605

aga ttg tct gta att gga atc aaa aac aga cct ggt att ata tca act 1872

Arg Leu Ser Val Ile Gly Ile Lys Asn Arg Pro Gly Ile Ile Ser Thr

610 615 620

gca cat aat aaa aat gaa ggt ggg tat ttc aga atg ctc aca tat aga 1920

Ala His Asn Lys Asn Glu Gly Gly Tyr Phe Arg Met Leu Thr Tyr Arg

625 630 635

ata acc gat tgt tta agt gat tgt tgt aca aat gtt agc aaa gaa gca 1968

Ile Thr Asp Cys Leu Ser Asp Cys Cys Thr Asn Val Ser Lys Glu Ala

640 645 650

gtg gat gag ttt tta cgg ata aaa gcc ttt aat ccc gct aaa gca att 2016

Val Asp Glu Phe Leu Arg Ile Lys Ala Phe Asn Pro Ala Lys Ala Ile

655 660 665

act atg tat aat ggg ata gat acc aat aaa ttt aaa ttt gat tta ttg 2064

Thr Met Tyr Asn Gly Ile Asp Thr Asn Lys Phe Lys Phe Asp Leu Leu

670 675 680 685

gca agg agg gaa att cga gac ggt att aat ata aaa aat gat gat ata 2112

Ala Arg Arg Glu Ile Arg Asp Gly Ile Asn Ile Lys Asn Asp Asp Ile

690 695 700

tta tta ctt gct gca ggt cgt tta acg tta gct aaa gat tat cct aat 2160

Leu Leu Leu Ala Ala Gly Arg Leu Thr Leu Ala Lys Asp Tyr Pro Asn

705 710 715

tta ttg aat gca atg act ctg ctt cct gaa cac ttt aaa ctt att att 2208

Leu Leu Asn Ala Met Thr Leu Leu Pro Glu His Phe Lys Leu Ile Ile

720 725 730

att ggt gat ggt gaa ttg cgt gac gaa att aat atg ctt ata aaa aaa 2256

Ile Gly Asp Gly Glu Leu Arg Asp Glu Ile Asn Met Leu Ile Lys Lys

735 740 745

ttg caa tta tct aat agg gtg tcc ttg ttg gga gtt aaa aaa aat att 2304

Leu Gln Leu Ser Asn Arg Val Ser Leu Leu Gly Val Lys Lys Asn Ile

750 755 760 765

gct ccc tat ttt tct gca tgt gat att ttt gtt ctc tct tct cgt tgg 2352

Ala Pro Tyr Phe Ser Ala Cys Asp Ile Phe Val Leu Ser Ser Arg Trp

770 775 780

gaa gga ttt gga tta gtc gtg gca gaa gct atg tca tgt gag cga att 2400

Glu Gly Phe Gly Leu Val Val Ala Glu Ala Met Ser Cys Glu Arg Ile

785 790 795

gtt gtt ggc acg gat tca ggg gga gta aga gaa gtt att ggt gac gat 2448

Val Val Gly Thr Asp Ser Gly Gly Val Arg Glu Val Ile Gly Asp Asp

800 805 810

gat ttt ctt gta ccc ata tct gat tca aca caa ctt gca agc aaa att 2496

Asp Phe Leu Val Pro Ile Ser Asp Ser Thr Gln Leu Ala Ser Lys Ile

815 820 825

gaa aaa ttg tct ttg agc cag ata cgt gat cac att ggt ttt cgg aat 2544

Glu Lys Leu Ser Leu Ser Gln Ile Arg Asp His Ile Gly Phe Arg Asn

830 835 840 845

cgt gag cgt att tta aaa aat ttc tca ata gat act att att atg cag 2592

Arg Glu Arg Ile Leu Lys Asn Phe Ser Ile Asp Thr Ile Ile Met Gln

850 855 860

tgg caa gaa ctc tat gga act ata att tgc tca aaa cat gaa agg 2637

Trp Gln Glu Leu Tyr Gly Thr Ile Ile Cys Ser Lys His Glu Arg

865 870 875

tagatttata tttggaacgt gtcttttgtt tgaatttaat tcaatctcaa ttgagatttt 2697

tgtatttcaa aaataccatc atagctaacg atgattggta tttattttaa gatgctttct 2757

ataaatatat tgacgttttt aatgcgccga aacgattggg ctgggaacag agaagtaaaa 2817

ctgttttgag aatgaagagt ttttgagatg tttatggata ttaaaaattg atccagtgaa 2877

ttaattattt ataataaatc aagatttaat gttaataaat gataatcttt tctgacactc 2937

atattaatta tgagtggtac gtttggtaaa cggtaaacta ttat atg aca gct aga 2993

Met Thr Ala Arg

880

aca act aaa gtt ttg cac tta caa tta ctc cca ctc tta agt ggc gtt 3041

Thr Thr Lys Val Leu His Leu Gln Leu Leu Pro Leu Leu Ser Gly Val

885 890 895

caa agg gta aca tta aac gaa att agt gcg tta tat act gat tat gat 3089

Gln Arg Val Thr Leu Asn Glu Ile Ser Ala Leu Tyr Thr Asp Tyr Asp

900 905 910

tat aca cta gtt tgc tca aaa aaa ggt cca cta aca aaa gca ttg ctg 3137

Tyr Thr Leu Val Cys Ser Lys Lys Gly Pro Leu Thr Lys Ala Leu Leu

915 920 925

gaa tat gat gtc gat tgt cat tgt atc ccc gaa ctt acg aga gaa att 3185

Glu Tyr Asp Val Asp Cys His Cys Ile Pro Glu Leu Thr Arg Glu Ile

930 935 940

acc gta aag aat gat ttt aaa gca ttg ttc aag ctt tat aag ttc ata 3233

Thr Val Lys Asn Asp Phe Lys Ala Leu Phe Lys Leu Tyr Lys Phe Ile

945 950 955 960

aaa aaa gaa aaa ttt gac att gtg cat aca cat tct tca aaa aca ggt 3281

Lys Lys Glu Lys Phe Asp Ile Val His Thr His Ser Ser Lys Thr Gly

965 970 975

att ttg ggg cga gtt gct gcc aaa tta gca cgt gtt gga aag gtg atc 3329

Ile Leu Gly Arg Val Ala Ala Lys Leu Ala Arg Val Gly Lys Val Ile

980 985 990

cac act gta cat ggt ttt tct ttt cca gcc gca tct agt aaa aaa agt 3377

His Thr Val His Gly Phe Ser Phe Pro Ala Ala Ser Ser Lys Lys Ser

995 1000 1005

tat tac ctt tat ttt ttc atg gaa tgg ata gca aag ttc ttt acg gat 3425

Tyr Tyr Leu Tyr Phe Phe Met Glu Trp Ile Ala Lys Phe Phe Thr Asp

1010 1015 1020

aag tta atc gtc ttg aat gta gat gat gaa tat ata gca ata aac aaa 3473

Lys Leu Ile Val Leu Asn Val Asp Asp Glu Tyr Ile Ala Ile Asn Lys

1025 1030 1035 1040

tta aaa ttc aag cgg gat aaa gtt ttt tta att cct aat gga gta gac 3521

Leu Lys Phe Lys Arg Asp Lys Val Phe Leu Ile Pro Asn Gly Val Asp

1045 1050 1055

act gat aag ttt tct cct tta gaa aat aaa att tat agt agc acc ttg 3569

Thr Asp Lys Phe Ser Pro Leu Glu Asn Lys Ile Tyr Ser Ser Thr Leu

1060 1065 1070

aat cta gta atg gtt ggt aga tta tcc aag caa aaa gat cct gag aca 3617

Asn Leu Val Met Val Gly Arg Leu Ser Lys Gln Lys Asp Pro Glu Thr

1075 1080 1085

tta ttg ctt gct gtt gaa aaa ctg ctg aat gaa aat gtt aat gtt aag 3665

Leu Leu Leu Ala Val Glu Lys Leu Leu Asn Glu Asn Val Asn Val Lys

1090 1095 1100

ctg aca ctt gta gga gat ggt gaa cta aaa gaa cag tta gaa agc agg 3713

Leu Thr Leu Val Gly Asp Gly Glu Leu Lys Glu Gln Leu Glu Ser Arg

1105 1110 1115 1120

ttc aaa cgg caa gat gga cgt ata att ttt cat gga tgg tca gat aac 3761

Phe Lys Arg Gln Asp Gly Arg Ile Ile Phe His Gly Trp Ser Asp Asn

1125 1130 1135

att gtt aat att tta aaa gtt aat gat ctt ttt ata tta cct tct ctt 3809

Ile Val Asn Ile Leu Lys Val Asn Asp Leu Phe Ile Leu Pro Ser Leu

1140 1145 1150

tgg gag ggt atg cca tta gca att tta gaa gca ttg agc tgt gga ctt 3857

Trp Glu Gly Met Pro Leu Ala Ile Leu Glu Ala Leu Ser Cys Gly Leu

1155 1160 1165

cca tgt ata gtc act aat att cca ggt aat aat agc tta ata gaa gat 3905

Pro Cys Ile Val Thr Asn Ile Pro Gly Asn Asn Ser Leu Ile Glu Asp

1170 1175 1180

ggc tat aat ggt tgt ttg ttt gaa att aga gat tgt cag tta tta tct 3953

Gly Tyr Asn Gly Cys Leu Phe Glu Ile Arg Asp Cys Gln Leu Leu Ser

1185 1190 1195 1200

caa aaa atc atg tca tat gtt ggt aag cca gaa ctg att gca cag caa 4001

Gln Lys Ile Met Ser Tyr Val Gly Lys Pro Glu Leu Ile Ala Gln Gln

1205 1210 1215

tct acc aat gca cga tca ttt att ctg aaa aat tat gga tta gtt aaa 4049

Ser Thr Asn Ala Arg Ser Phe Ile Leu Lys Asn Tyr Gly Leu Val Lys

1220 1225 1230

aga aat aat aag gtc aga cag cta tat gat aat taaatgaaac cgaaaagtta 4102

Arg Asn Asn Lys Val Arg Gln Leu Tyr Asp Asn

1235 1240

aaaaagaaca ggtttttcaa agtgaaaata aaattacagt ttttttattg caatgattaa 4162

cgtaacatct gcattacatt caagccgcac aaccccgcgg tgaccacccc tgacaggagt 4222

aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatggga cgcaacctcg 4282

cgctcaacat cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga 4342

cggaagaagt tattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag 4402

agttcgttga atctcttgaa acgcctcgtc gcatcctgtt aatgggttaa agcaggtgca 4462

ggcacggatg ctgctattga ttccctgaaa ccatatctcg ataaaggcga tatcatcatt 4522

gatgggtggg taataccttc tttcaggaca ccattcgtcg taaccgcgag ctttctgcac 4582

aaggctttac ttcatcggta cc 4604

<210>23

<211>247

<212>PRT

<213>大肠杆菌

<400>23

Val Asp Asp Asn Leu Lys Gly Gln Gly Ala Gly Lys Asn Phe Leu Ser

1 5 10 15

Leu Ile Lys Tyr Ser Glu Thr Asp Tyr Thr Ile Tyr Cys Asp Gln Asp

20 25 30

Asp Ile Trp Leu Glu Asn Lys Ile Phe Glu Leu Val Lys Tyr Ala Asn

35 40 45

Glu Ile Lys Leu Asn Val Ser Asp Ala Pro Ser Leu Val Tyr Ala Asp

50 55 60

Gly Tyr Ala Tyr Met Asp Gly Glu Gly Thr Ile Asp Phe Ser Gly Ile

65 70 75 80

Ser Asn Asn His Ala Asp Gln Leu Lys Asp Phe Leu Phe Phe Asn Gly

85 90 95

Gly Tyr Gln Gly Cys Ser Ile Met Phe Asn Arg Ala Met Thr Lys Phe

100 105 110

Leu Leu Asn Tyr Arg Gly Phe Val Tyr Leu His Asp Asp Ile Thr Thr

115 120 125

Leu Ala Ala Tyr Ala Leu Gly Lys Val Tyr Phe Leu Pro Lys Tyr Leu

130 135 140

Met Leu Tyr Arg Gln His Thr Asn Ala Val Thr Gly Ile Lys Thr Phe

145 150 155 160

Arg Asn Gly Leu Thr Ser Lys Phe Lys Ser Pro Val Asn Tyr Leu Leu

165 170 175

Ser Arg Lys His Tyr Gln Val Lys Lys Ser Phe Phe Glu Cys Asn Ser

180 185 190

Ser Ile Leu Ser Glu Thr Asn Lys Lys Val Phe Leu Asp Phe Ile Ser

195 200 205

Phe Cys Glu Ser Asn Asn Lys Phe Thr Asp Phe Phe Lys Leu Trp Arg

210 215 220

Gly Gly Phe Arg Leu Asn Asn Ser Arg Thr Lys Leu Leu Leu Lys Phe

225 230 235 240

Leu Ile Arg Arg Lys Phe Ser

245

<210>24

<211>261

<212>PRT

<213>大肠杆菌

<400>24

Met Ile Ser Ile Leu Thr Pro Thr Phe Asn Arg Gln His Thr Leu Ser

1 5 10 15

Arg Leu Phe Asn Ser Leu Ile Leu Gln Thr Asp Lys Asp Phe Glu Trp

20 25 30

Ile Ile Ile Asp Asp Gly Ser Ile Asp Ala Thr Ala Val Leu Val Glu

35 40 45

Asp Phe Arg Lys Lys Cys Asp Phe Asp Leu Ile Tyr Cys Tyr Gln Glu

50 55 60

Asn Asn Gly Lys Pro Met Ala Leu Asn Ala Gly Val Lys Ala Cys Arg

65 70 75 80

Gly Asp Tyr Ile Phe Ile Val Asp Ser Asp Asp Ala Leu Thr Pro Asp

85 90 95

Ala Ile Lys Leu Ile Lys Glu Ser Ile His Asp Cys Leu Ser Glu Lys

100 105 110

Glu Ser Phe Ser Gly Val Gly Phe Arg Lys Ala Tyr Ile Lys Gly Gly

115 120 125

Ile Ile Gly Asn Asp Leu Asn Asn Ser Ser Glu His Ile Tyr Tyr Leu

130 135 140

Asn Ala Thr Glu Ile Ser Asn Leu Ile Asn Gly Asp Val Ala Tyr Cys

145 150 155 160

Phe Lys Lys Glu Ser Leu Val Lys Asn Pro Phe Pro Arg Ile Glu Asp

165 170 175

Glu Lys Phe Val Pro Glu Leu Tyr Ile Trp Asn Lys Ile Thr Asp Lys

180 185 190

Ala Lys Ile Arg Phe Asn Ile Ser Lys Val Ile Tyr Leu Cys Glu Tyr

195 200 205

Leu Asp Asp Gly Leu Ser Lys Asn Phe His Asn Gln Leu Lys Lys Tyr

210 215 220

Pro Lys Gly Phe Lys Ile Tyr Tyr Lys Asp Gln Arg Lys Arg Glu Lys

225 230 235 240

Thr Tyr Ile Lys Lys Thr Lys Met Leu Ile Arg Tyr Leu Gln Cys Cys

245 250 255

Tyr Tyr Glu Lys Ile

260

<210>25

<211>368

<212>PRT

<213>大肠杆菌

<400>25

Met Lys Ile Leu Phe Val Ile Thr Gly Leu Gly Leu Gly Gly Ala Glu

1 5 10 15

Lys Gln Val Cys Leu Leu Ala Asp Lys Leu Ser Leu Ser Gly His His

20 25 30

Val Lys Ile Ile Ser Leu Gly His Met Ser Asn Asn Lys Val Phe Pro

35 40 45

Ser Glu Asn Asn Val Asn Val Ile Asn Val Asn Met Ser Lys Asn Ile

50 55 60

Ser Gly Val Ile Lys Gly Cys Val Arg Ile Arg Asp Val Ile Ala Asn

65 70 75 80

Phe Lys Pro Asp Ile Val His Ser His Met Phe His Ala Asn Ile Ile

85 90 95

Thr Arg Leu Ser Val Ile Gly Ile Lys Asn Arg Pro Gly Ile Ile Ser

100 105 110

Thr Ala His Asn Lys Asn Glu Gly Gly Tyr Phe Arg Met Leu Thr Tyr

115 120 125

Arg Ile Thr Asp Cys Leu Ser Asp Cys Cys Thr Asn Val Ser Lys Glu

130 135 140

Ala Val Asp Glu Phe Leu Arg Ile Lys Ala Phe Asn Pro Ala Lys Ala

145 150 155 160

Ile Thr Met Tyr Asn Gly Ile Asp Thr Asn Lys Phe Lys Phe Asp Leu

165 170 175

Leu Ala Arg Arg Glu Ile Arg Asp Gly Ile Asn Ile Lys Asn Asp Asp

180 185 190

Ile Leu Leu Leu Ala Ala Gly Arg Leu Thr Leu Ala Lys Asp Tyr Pro

195 200 205

Asn Leu Leu Asn Ala Met Thr Leu Leu Pro Glu His Phe Lys Leu Ile

210 215 220

Ile Ile Gly Asp Gly Glu Leu Arg Asp Glu Ile Asn Met Leu Ile Lys

225 230 235 240

Lys Leu Gln Leu Ser Asn Arg Val Ser Leu Leu Gly Val Lys Lys Asn

245 250 255

Ile Ala Pro Tyr Phe Ser Ala Cys Asp Ile Phe Val Leu Ser Ser Arg

260 265 270

Trp Glu Gly Phe Gly Leu Val Val Ala Glu Ala Met Ser Cys Glu Arg

275 280 285

Ile Val Val Gly Thr Asp Ser Gly Gly Val Arg Glu Val Ile Gly Asp

290 295 300

Asp Asp Phe Leu Val Pro Ile Ser Asp Ser Thr Gln Leu Ala Ser Lys

305 310 315 320

Ile Glu Lys Leu Ser Leu Ser Gln Ile Arg Asp His Ile Gly Phe Arg

325 330 335

Asn Arg Glu Arg Ile Leu Lys Asn Phe Ser Ile Asp Thr Ile Ile Met

340 345 350

Gln Trp Gln Glu Leu Tyr Gly Thr Ile Ile Cys Ser Lys His Glu Arg

355 360 365

<210>26

<211>367

<212>PRT

<213>大肠杆菌

<400>26

Met Thr Ala Arg Thr Thr Lys Val Leu His Leu Gln Leu Leu Pro Leu

1 5 10 15

Leu Ser Gly Val Gln Arg Val Thr Leu Asn Glu Ile Ser Ala Leu Tyr

20 25 30

Thr Asp Tyr Asp Tyr Thr Leu Val Cys Ser Lys Lys Gly Pro Leu Thr

35 40 45

Lys Ala Leu Leu Glu Tyr Asp Val Asp Cys His Cys Ile Pro Glu Leu

50 55 60

Thr Arg Glu Ile Thr Val Lys Asn Asp Phe Lys Ala Leu Phe Lys Leu

65 70 75 80

Tyr Lys Phe Ile Lys Lys Glu Lys Phe Asp Ile Val His Thr His Ser

85 90 95

Ser Lys Thr Gly Ile Leu Gly Arg Val Ala Ala Lys Leu Ala Arg Val

100 105 110

Gly Lys Val Ile His Thr Val His Gly Phe Ser Phe Pro Ala Ala Ser

115 120 125

Ser Lys Lys Ser Tyr Tyr Leu Tyr Phe Phe Met Glu Trp Ile Ala Lys

130 135 140

Phe Phe Thr Asp Lys Leu Ile Val Leu Asn Val Asp Asp Glu Tyr Ile

145 150 155 160

Ala Ile Asn Lys Leu Lys Phe Lys Arg Asp Lys Val Phe Leu Ile Pro

165 170 175

Asn Gly Val Asp Thr Asp Lys Phe Ser Pro Leu Glu Asn Lys Ile Tyr

180 185 190

Ser Ser Thr Leu Asn Leu Val Met Val Gly Arg Leu Ser Lys Gln Lys

195 200 205

Asp Pro Glu Thr Leu Leu Leu Ala Val Glu Lys Leu Leu Asn Glu Asn

210 215 220

Val Asn Val Lys Leu Thr Leu Val Gly Asp Gly Glu Leu Lys Glu Gln

225 230 235 240

Leu Glu Ser Arg Phe Lys Arg Gln Asp Gly Arg Ile Ile Phe His Gly

245 250 255

Trp Ser Asp Asn Ile Val Asn Ile Leu Lys Val Asn Asp Leu Phe Ile

260 265 270

Leu Pro Ser Leu Trp Glu Gly Met Pro Leu Ala Ile Leu Glu Ala Leu

275 280 285

Ser Cys Gly Leu Pro Cys Ile Val Thr Asn Ile Pro Gly Asn Asn Ser

290 295 300

Leu Ile Glu Asp Gly Tyr Asn Gly Cys Leu Phe Glu Ile Arg Asp Cys

305 310 315 320

Gln Leu Leu Ser Gln Lys Ile Met Ser Tyr Val Gly Lys Pro Glu Leu

325 330 335

Ile Ala Gln Gln Ser Thr Asn Ala Arg Ser Phe Ile Leu Lys Asn Tyr

340 345 350

Gly Leu Val Lys Arg Asn Asn Lys Val Arg Gln Leu Tyr Asp Asn

355 360 365

<210>27

<211>1272

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(319)..(1269)

<220>

<221>CDS

<222>(3)..(215)

<400>27

cc ggg aag cac tcg gcg ctg att gtt gca cat cgt ctg acc acc gcg 47

Gly Lys His Ser Ala Leu Ile Val Ala His Arg Leu Thr Thr Ala

1 5 10 15

caa cgc tgc gat ctg att gcc gtt att gat aag ggg tta ctt gcg gaa 95

Gln Arg Cys Asp Leu Ile Ala Val Ile Asp Lys Gly Leu Leu Ala Glu

20 25 30

tac gga acc cac gaa cag ctg tta tct gcg ggc ggc ctc tat acc cgc 143

Tyr Gly Thr His Glu Gln Leu Leu Ser Ala Gly Gly Leu Tyr Thr Arg

35 40 45

tta tgg cat gac agc gtc agc agt act gct ctc cat cgc cag cac aac 191

Leu Trp His Asp Ser Val Ser Ser Thr Ala Leu His Arg Gln His Asn

50 55 60

atg aag gag gaa acc ccg gga tag ttactggaca cgtaatgtat taaaaacaca 245

Met Lys Glu Glu Thr Pro Gly

65 70

gtcagaagcg gcggtaccgt gaatagccgc tttaattatt tatactgaca tccttaattt 305

ttaaagagta tga atg ctg aac atg caa caa cat ctc tct gct atc gcc 354

Met Leu Asn Met Gln Gln His Leu Ser Ala Ile Ala

75 80

agc ctg cgc aac caa ctg gca gcg ggc cac att gct aac ctt act gac 402

Ser Leu Arg Asn Gln Leu Ala Ala Gly His Ile Ala Asn Leu Thr Asp

85 90 95

ttc tgg cgc gaa gct gag tcg ctg aat gtt cct ctt gtg acg cca gtc 450

Phe Trp Arg Glu Ala Glu Ser Leu Asn Val Pro Leu Val Thr Pro Val

100 105 110 115

gaa gga gcg gaa gat gag cga gaa gtg acc ttt ctg tgg cgc gcc cga 498

Glu Gly Ala Glu Asp Glu Arg Glu Val Thr Phe Leu Trp Arg Ala Arg

120 125 130

cat cct ctg cag ggc gtt tat ctg cgt ctg aac cgg gtg acg gat aaa 546

His Pro Leu Gln Gly Val Tyr Leu Arg Leu Asn Arg Val Thr Asp Lys

135 140 145

gag cac gta gaa aaa gga atg atg agc gcc ctt ccc gaa acg gat atc 594

Glu His Val Glu Lys Gly Met Met Ser Ala Leu Pro Glu Thr Asp Ile

150 155 160

tgg aca ctg aca ctg cgt tta ccc gca agt tac tgc ggc tcc tat tcg 642

Trp Thr Leu Thr Leu Arg Leu Pro Ala Ser Tyr Cys Gly Ser Tyr Ser

165 170 175

ctg ctg gaa atc ccc ccc ggc act acg gct gag acg att gca ctg tcc 690

Leu Leu Glu Ile Pro Pro Gly Thr Thr Ala Glu Thr Ile Ala Leu Ser

180 185 190 195

gga ggc cgt ttt gcc acc ctt gcc gga aag gcc gat ccg cta aac aaa 738

Gly Gly Arg Phe Ala Thr Leu Ala Gly Lys Ala Asp Pro Leu Asn Lys

200 205 210

atg ccg gag atc aac gtt cgg gga aac gca aag gaa tca gtg ctg aca 786

Met Pro Glu Ile Asn Val Arg Gly Asn Ala Lys Glu Ser Val Leu Thr

215 220 225

ctt gat aaa gct ccc gcc ctg tcg gaa tgg aac ggc ggc ttc cac acc 834

Leu Asp Lys Ala Pro Ala Leu Ser Glu Trp Asn Gly Gly Phe His Thr

230 235 240

gga caa ctg ctt acc tcc atg cgc att atc gcc ggg aaa tct cgc cag 882

Gly Gln Leu Leu Thr Ser Met Arg Ile Ile Ala Gly Lys Ser Arg Gln

245 250 255

gtt cgg ctc tat att ccg gat gtt gat att tct cag ccc ctc ggg ctg 930

Val Arg Leu Tyr Ile Pro Asp Val Asp Ile Ser Gln Pro Leu Gly Leu

260 265 270 275

gtc gtg ctg ccc gat ggt gaa acc tgg ttt gat cac ctt ggc gta tgc 978

Val Val Leu Pro Asp Gly Glu Thr Trp Phe Asp His Leu Gly Val Cys

280 285 290

gcg gca att gac gcc gcc ata aat aat ggg cgc atc gtg ccc gtg gct 1026

Ala Ala Ile Asp Ala Ala Ile Asn Asn Gly Arg Ile Val Pro Val Ala

295 300 305

gta ctg ggc att gac aac att aat gaa cat gaa cgc act gag ata ctc 1074

Val Leu Gly Ile Asp Asn Ile Asn Glu His Glu Arg Thr Glu Ile Leu

310 315 320

ggc ggg cgc agc aaa ctg ata aag gat atc gcc gga cat ctg ctg ccg 1122

Gly Gly Arg Ser Lys Leu Ile Lys Asp Ile Ala Gly His Leu Leu Pro

325 330 335

atg att cgc gct gaa caa ccg cag cgt cag tgg gca gac cgt tcg cgc 1170

Met Ile Arg Ala Glu Gln Pro Gln Arg Gln Trp Ala Asp Arg Ser Arg

340 345 350 355

aca gtg ctg gcc ggg cag agc ctc ggc ggg atc agt gcg cta atg ggg 1218

Thr Val Leu Ala Gly Gln Ser Leu Gly Gly Ile Ser Ala Leu Met Gly

360 365 370

gct cgt tac gca ccg gaa acg ttc ggt ctg gtg ctc agc cac tct cct 1266

Ala Arg Tyr Ala Pro Glu Thr Phe Gly Leu Val Leu Ser His Ser Pro

375 380 385

caa tgc 1272

Gln

<210>28

<211>70

<212>PRT

<213>大肠杆菌

<400>28

Gly Lys His Ser Ala Leu Ile Val Ala His Arg Leu Thr Thr Ala Gln

1 5 10 15

Arg Cys Asp Leu Ile Ala Val Ile Asp Lys Gly Leu Leu Ala Glu Tyr

20 25 30

Gly Thr His Glu Gln Leu Leu Ser Ala Gly Gly Leu Tyr Thr Arg Leu

35 40 45

Trp His Asp Ser Val Ser Ser Thr Ala Leu His Arg Gln His Asn Met

50 55 60

Lys Glu Glu Thr Pro Gly

65 70

<210>29

<211>317

<212>PRT

<213>大肠杆菌

<400>29

Met Leu Asn Met Gln Gln His Leu Ser Ala Ile Ala Ser Leu Arg Asn

1 5 10 15

Gln Leu Ala Ala Gly His Ile Ala Asn Leu Thr Asp Phe Trp Arg Glu

20 25 30

Ala Glu Ser Leu Asn Val Pro Leu Val Thr Pro Val Glu Gly Ala Glu

35 40 45

Asp Glu Arg Glu Val Thr Phe Leu Trp Arg Ala Arg His Pro Leu Gln

50 55 60

Gly Val Tyr Leu Arg Leu Asn Arg Val Thr Asp Lys Glu His Val Glu

65 70 75 80

Lys Gly Met Met Ser Ala Leu Pro Glu Thr Asp Ile Trp Thr Leu Thr

85 90 95

Leu Arg Leu Pro Ala Ser Tyr Cys Gly Ser Tyr Ser Leu Leu Glu Ile

100 105 110

Pro Pro Gly Thr Thr Ala Glu Thr Ile Ala Leu Ser Gly Gly Arg Phe

115 120 125

Ala Thr Leu Ala Gly Lys Ala Asp Pro Leu Asn Lys Met Pro Glu Ile

130 135 140

Asn Val Arg Gly Asn Ala Lys Glu Ser Val Leu Thr Leu Asp Lys Ala

145 150 155 160

Pro Ala Leu Ser Glu Trp Asn Gly Gly Phe His Thr Gly Gln Leu Leu

165 170 175

Thr Ser Met Arg Ile Ile Ala Gly Lys Ser Arg Gln Val Arg Leu Tyr

180 185 190

Ile Pro Asp Val Asp Ile Ser Gln Pro Leu Gly Leu Val Val Leu Pro

195 200 205

Asp Gly Glu Thr Trp Phe Asp His Leu Gly Val Cys Ala Ala Ile Asp

210 215 220

Ala Ala Ile Asn Asn Gly Arg Ile Val Pro Val Ala Val Leu Gly Ile

225 230 235 240

Asp Asn Ile Asn Glu His Glu Arg Thr Glu Ile Leu Gly Gly Arg Ser

245 250 255

Lys Leu Ile Lys Asp Ile Ala Gly His Leu Leu Pro Met Ile Arg Ala

260 265 270

Glu Gln Pro Gln Arg Gln Trp Ala Asp Arg Ser Arg Thr Val Leu Ala

275 280 285

Gly Gln Ser Leu Gly Gly Ile Ser Ala Leu Met Gly Ala Arg Tyr Ala

290 295 300

Pro Glu Thr Phe Gly Leu Val Leu Ser His Ser Pro Gln

305 310 315

<210>30

<211>4039

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(1)..(285)

<220>

<221>CDS

<222>(370)..(1326)

<400>30

cct tca atg tgg tgg acg cca gaa aga acc agt cga cca ggc ttg ttc 48

Pro Ser Met Trp Trp Thr Pro Glu Arg Thr Ser Arg Pro Gly Leu Phe

1 5 10 15

agc gaa acc gat acc tca tgg gtg agt gag cat ctg ctt tct gcc cca 96

Ser Glu Thr Asp Thr Ser Trp Val Ser Glu His Leu Leu Ser Ala Pro

20 25 30

ccg cag ggc gta cgt atc agc ctg tgc gtg gga tcg ctg gaa ggt tcg 144

Pro Gln Gly Val Arg Ile Ser Leu Cys Val Gly Ser Leu Glu Gly Ser

35 40 45

aca gtg cct cac gtt cag cag ctt cac cag cgg ctg att acc gct ggc 192

Thr Val Pro His Val Gln Gln Leu His Gln Arg Leu Ile Thr Ala Gly

50 55 60

gtc gaa agc cat tgc gca atc tac acc ggt ggt cac gat tac gca tgg 240

Val Glu Ser His Cys Ala Ile Tyr Thr Gly Gly His Asp Tyr Ala Trp

65 70 75 80

tgg cgc ggt gca ctg att gac ggg att ggt tta cta cag ggt tga 285

Trp Arg Gly Ala Leu Ile Asp Gly Ile Gly Leu Leu Gln Gly

85 90 95

gttgacccac aaacactttc aggaaacggt acagacttcc tgaataaatc aaatagtcac 345

ctgcggaaaa ggaataatca tcag atg tat gcc cgc gag tat cgc tca aca 396

Met Tyr Ala Arg Glu Tyr Arg Ser Thr

100

cgc ccg cat aaa gcg att ttc ttt cat ctt tct tgc ctc acc ctt atc 444

Arg Pro His Lys Ala Ile Phe Phe His Leu Ser Cys Leu Thr Leu Ile

105 110 115 120

tgt agt gcg caa gtt tat gcg aag ccg gat atg cgg cca ctg ggg ccg 492

Cys Ser Ala Gln Val Tyr Ala Lys Pro Asp Met Arg Pro Leu Gly Pro

125 130 135

aat ata gcc gat aaa ggc tcc gtg ttt tac cat ttc agc gtc acc tct 540

Asn Ile Ala Asp Lys Gly Ser Val Phe Tyr His Phe Ser Val Thr Ser

140 145 150

ttc gac tct gtc gat ggc aca cgc cat tat cgg gta tgg acg gcc gtg 588

Phe Asp Ser Val Asp Gly Thr Arg His Tyr Arg Val Trp Thr Ala Val

155 160 165

ccg aat aca acc gca ccg gca tcg ggt tac ccg att tta tat atg ctt 636

Pro Asn Thr Thr Ala Pro Ala Ser Gly Tyr Pro Ile Leu Tyr Met Leu

170 175 180

gac ggt aac gca gtt atg gat cgc ctg gat gac gaa ctg ctc aaa caa 684

Asp Gly Asn Ala Val Met Asp Arg Leu Asp Asp Glu Leu Leu Lys Gln

185 190 195 200

ttg tca gaa aaa aca ccg cca gtg atc gtg gct gtc ggg tat cag acc 732

Leu Ser Glu Lys Thr Pro Pro Val Ile Val Ala Val Gly Tyr Gln Thr

205 210 215

aac ctc cct ttc gat ctc aac agc agg gct tac gac tat acg cca gca 780

Asn Leu Pro Phe Asp Leu Asn Ser Arg Ala Tyr Asp Tyr Thr Pro Ala

220 225 230

gca gaa agc aga aaa aca gat ctc cac tca ggg cgt ttt agc cgt aag 828

Ala Glu Ser Arg Lys Thr Asp Leu His Ser Gly Arg Phe Ser Arg Lys

235 240 245

agt ggt ggc agc aac aac ttc cgc cag tta ctg gaa acg cgt att gcc 876

Ser Gly Gly Ser Asn Asn Phe Arg Gln Leu Leu Glu Thr Arg Ile Ala

250 255 260

cca aaa gtg gaa cag gga ctg aat atc gat cgg caa cgc cgc ggc tta 924

Pro Lys Val Glu Gln Gly Leu Asn Ile Asp Arg Gln Arg Arg Gly Leu

265 270 275 280

tgg ggg cac tcc tac ggc ggc ctc ttc gtg ctg gat tcc tgg ctg tcc 972

Trp Gly His Ser Tyr Gly Gly Leu Phe Val Leu Asp Ser Trp Leu Ser

285 290 295

tcc tct tac ttc cgg tcg tac tac agc gcc agc ccg tcg ttg ggc aga 1020

Ser Ser Tyr Phe Arg Ser Tyr Tyr Ser Ala Ser Pro Ser Leu Gly Arg

300 305 310

ggt tat gat gct ttg cta agc cgc gtt acg gcg gtt gag cct ctg caa 1068

Gly Tyr Asp Ala Leu Leu Ser Arg Val Thr Ala Val Glu Pro Leu Gln

315 320 325

ttc tgc gcc aaa cac ctg gcg ara atg gaa ggc tcg gcg aca cag ggt 1116

Phe Cys Ala Lys His Leu Ala Ile Met Glu Gly Ser Ala Thr Gln Gly

330 335 340

gat aac cgg gaa acg cat gct gtc ggg gtg ctg tcg aaa att cat acc 1164

Asp Asn Arg Glu Thr His Ala Val Gly Val Leu Ser Lys Ile His Thr

345 350 355 360

acc ctc act ata ctg aaa gat aaa ggc gtc aat gcc gta ttt tgg gat 1212

Thr Leu Thr Ile Leu Lys Asp Lys Gly Val Asn Ala Val Phe Trp Asp

365 370 375

ttc ccc aac ctg gga cac ggg ccg atg ttc aat gcc tcc ttt cgc cag 1260

Phe Pro Asn Leu Gly His Gly Pro Met Phe Asn Ala Ser Phe Arg Gln

380 385 390

gca ctg tta gat atc agt ggt gaa aac gca aat tac aca gca ggt tgt 1308

Ala Leu Leu Asp Ile Ser Gly Glu Asn Ala Asn Tyr Thr Ala Gly Cys

395 400 405

cat gag tta agc cac taa acactgcccg cttttacgcg ggcagtacgc 1356

His Glu Leu Ser His

410

ctgaaacact acgatcagaa tgatgcggta actccggcat agtaagcccg gcctggctcg 1416

ttataggtat tcgccccttc agaagatcgg aagatctgtt tattgaggat attactgacg 1476

ccgacattaa gacgcagatt tttattaata tcgtaattga agttcgtccc caccagtgaa 1536

taagcgccca gctctttacc tgacagaccg ccagtatctt cactgcgggt ttccgcatga 1596

gtacgcggtt tttgtctgcc atataacgtc cagttgacgc tggcagaaaa cgcctgggtg 1656

atggtccagt taagcgagtt attgatagta tatttcggga tgaccgacag aggattaccg 1716

gtgtcttttt gctccgaagt gatcatccat gtggcattgg tattccagtt cagacgatct 1776

ttcaccagtg ggaaagacat actggcttcg ataccgtcca ccagagcttt cccgccattc 1836

tgccacttga ggatatatgc gcctgaagcg gtttgcccga taacgttatc cccggccacg 1896

atcttattct ggtaatcatt gcggaagtag gtcacacttg cgtggtaatc ttcccaggtg 1956

aactccagcc caatttcttt attgacgctg atttccggat cgagatcttt attaccgatc 2016

aggtagcacc cgcctgatgt aatatctttt ggacagccat tgcctttcga gtagagcaga 2076

tagccttcac tggattgata caggtttggg gctttaaagg ttcgggcaac ccctgctttg 2136

actttgaaat aatcgcccaa ttcctgcgaa agattcagac tggggctgaa gttcccgccg 2196

gagtcgctga gataatcaaa gcgcaggccg ggaatgatat tcgtgccagg aaccggctca 2256

atgttatctt caatatacag cgcactgatt tgagaatgat ttttactgct gcgatccgca 2316

gcagagccag aaataccgct gatatcactg tcattcaccg tcaggctggt agaggaagga 2376

tcatcgagct tatcgcggtt ccactctgca ccaacggtca gcgtttgatc aaccatcaca 2436

ttcaaaggaa tattaagctc gccgctggtt cgccaggaac tcaggcgatt ggtcgtaaac 2496

ttttcacccg ctaaaatacg cccttcacca ccgccggata atccttcatt catgcgggta 2556

ttattggttt tctcgtaata aacaccaaag cgactttgtc cccagtccca gataccatta 2616

tgcgtaatgc cataattctg tcggtacagg cggctcgtct cttgtgccgg attttgccag 2676

gcttttcggt aactgcactg gaagaactgt tttgcgtatc gccgcataga tattcccctg 2736

gcggctatat ccggcttcga aatcgagaat ctgctgcgga tttaatttcc acgagacaac 2796

gccgttaata tctttgttac gtaccccttc atgcccggct gcgtttttcg taccgaccgg 2856

agaattaata tcccaactgt cagcatccgt tttattcaga ttaccataca aacgcgtggt 2916

aagagcatta ccagccagag gcccactaag gctgaaattg gcgcgacgcg tagcgccctc 2976

atcgctactt tccggctgat tggtgtataa cgacagcgaa ccgtgccagt cgttggtggg 3036

acgtttggta atgatgttca ccaccccccc ggctgccccc gaaccgtagc gcgccgccgc 3096

agggccgcgg atcacttcaa tacgctcaac ctgttccggt ggcacccagt tggtgtcacc 3156

gcgggtatca cgctctccac gccagctata acgcacggag ttacgtgacg tcaccggtac 3216

accatcaatt aaaattaagg tgttttccgg ccccatacca cgaatatcga tctggcggtt 3276

gttaccgcgt gtgcccgagg cgctattgcc ggtaagattg acgccaggca ttttacgaat 3336

aatatctgaa aggtcgttta ccggaggggt ctttttaata tcctcgctgg taataaccga 3396

cacgcccggc tgctgtttta atacctgctc agcggtggct tccaccacca gagtctcgtc 3456

attatcatcg tcggaggatt tggctactga tacctggcta ttcaacccaa ccaggagcac 3516

agttagcgac cagaggattt tgttaattct catacctatt ccctaataaa tgcctaactt 3576

aaaatgtttg atcgttaagc tcacatcctt gccagatatt ttttactgcc attattgttt 3636

ttatataaga atgataatta atatcattta gcaaaagaaa aagcaatccc tcacaagata 3696

aatatatcga tttttcataa atatcaaatt gatatataac atatgttttt tatttcattg 3756

tacttcagtc aaataaattt ctgaagcact gctagtagtg ccagttcagc tttctttttg 3816

actcattccg gcaaagtcag taccgttcat cttttgtact gatgttgcca ctggaaaatc 3876

ggtgcgcttg tcgatcatcg ggaattttgt cacaatttct aacggatagt gttcacattg 3936

tttctaacct gcattttcag acacgggcgc tgcttatgta tataagatca gcatcactag 3996

gtctttctgc aacactactg ctttcaacaa ggtcaggcat ttc 4039

<210>31

<211>94

<212>PRT

<213>大肠杆菌

<400>31

Pro Ser Met Trp Trp Thr Pro Glu Arg Thr Ser Arg Pro Gly Leu Phe

1 5 10 15

Ser Glu Thr Asp Thr Ser Trp Val Ser Glu His Leu Leu Ser Ala Pro

20 25 30

Pro Gln Gly Val Arg Ile Ser Leu Cys Val Gly Ser Leu Glu Gly Ser

35 40 45

Thr Val Pro His Val Gln Gln Leu His Gln Arg Leu Ile Thr Ala Gly

50 55 60

Val Glu Ser His Cys Ala Ile Tyr Thr Gly Gly His Asp Tyr Ala Trp

65 70 75 80

Trp Arg Gly Ala Leu Ile Asp Gly Ile Gly Leu Leu Gln Gly

85 90

<210>32

<211>318

<212>PRT

<213>大肠杆菌

<400>32

Met Tyr Ala Arg Glu Tyr Arg Ser Thr Arg Pro His Lys Ala Ile Phe

1 5 10 15

Phe His Leu Ser Cys Leu Thr Leu Ile Cys Ser Ala Gln Val Tyr Ala

20 25 30

Lys Pro Asp Met Arg Pro Leu Gly Pro Asn Ile Ala Asp Lys Gly Ser

35 40 45

Val Phe Tyr His Phe Ser Val Thr Ser Phe Asp Ser Val Asp Gly Thr

50 55 60

Arg His Tyr Arg Val Trp Thr Ala Val Pro Asn Thr Thr Ala Pro Ala

65 70 75 80

Ser Gly Tyr Pro Ile Leu Tyr Met Leu Asp Gly Asn Ala Val Met Asp

85 90 95

Arg Leu Asp Asp Glu Leu Leu Lys Gln Leu Ser Glu Lys Thr Pro Pro

100 105 110

Val Ile Val Ala Val Gly Tyr Gln Thr Asn Leu Pro Phe Asp Leu Asn

115 120 125

Ser Arg Ala Tyr Asp Tyr Thr Pro Ala Ala Glu Ser Arg Lys Thr Asp

130 135 140

Leu His Ser Gly Arg Phe Ser Arg Lys Ser Gly Gly Ser Asn Asn Phe

145 150 155 160

Arg Gln Leu Leu Glu Thr Arg Ile Ala Pro Lys Val Glu Gln Gly Leu

165 170 175

Asn Ile Asp Arg Gln Arg Arg Gly Leu Trp Gly His Ser Tyr Gly Gly

180 185 190

Leu Phe Val Leu Asp Ser Trp Leu Ser Ser Ser Tyr Phe Arg Ser Tyr

195 200 205

Tyr Ser Ala Ser Pro Ser Leu Gly Arg Gly Tyr Asp Ala Leu Leu Ser

210 215 220

Arg Val Thr Ala Val Glu Pro Leu Gln Phe Cys Ala Lys His Leu Ala

225 230 235 240

Ile Met Glu Gly Ser Ala Thr Gln Gly Asp Asn Arg Glu Thr His Ala

245 250 255

Val Gly Val Leu Ser Lys Ile His Thr Thr Leu Thr Ile Leu Lys Asp

260 265 270

Lys Gly Val Asn Ala Val Phe Trp Asp Phe Pro Asn Leu Gly His Gly

275 280 285

Pro Met Phe Asn Ala Ser Phe Arg Gln Ala Leu Leu Asp Ile Ser Gly

290 295 300

Glu Asn Ala Asn Tyr Thr Ala Gly Cys His Glu Leu Ser His

305 310 315

<210>33

<211>3292

<212>DNA

<213>大肠杆菌

<400>33

ccgctgcggt tgattgccgg atgcggcgtg aacgccttat ccggcctaca atcattgcaa 60

attcaataaa ttgcagcgtt ctgtaggctg gataagatgc gtcagcatcg catccggcaa 120

aggcagatct cagcgatagc gccggcttag tcagatttaa tctgcgcgcg tggtggatat 180

tttttcagga tctccatata cgcgtgcatt tcggtctgta gcggtacacc catcggaata 240

tggcgcacgc cgatagagtc gctttcctgc ggatcggtgt acaggttaaa caccgacgat 300

cccgccgttt gcattactgt gccggtgaat ccaccctgat atccgctctg ggtataagcg 360

taaggttgct gaatcaggac gtgatacttg aactcatcca tacgcacagc agaaagttta 420

ccgttgagga agtagtgctc ggccttacgg ttagactgac catttgttcc caggaagaaa 480

gatgtctggt ccacaccatc gataaaggtg gttttcggta ctaaattcgc tactttcgcc 540

ccaggatgcc ccgccagatc cagcgccgta gggaagagat ccgccagatc gacaatgccg 600

tcagatttac gcggttggat catgcctttc cagtaaacga aggttggcac acgaacgccg 660

ccttcccatg tagaaccttt cgcaccgcgg aacggcgtgc gaccgtgcgg cggaacttcg 720

gcttccggac cgttatcaga ggtgaacaca atcagcgtgt tatcaagctg accgtttttc 780

tccagtgctt tatacagatt ggcgaagata tcgttcatct ccaccatgca gtcgccgtaa 840

gaggtgcgcg ccggagagct acccgcatat ttggcgttcg ggtagttatc gaagtgacag 900

ccgcgagtgc cgtaatagag gaagaaaggc ttatcactct tcgccatctt gtcgaggaac 960

ttaacgccat attccatcca gcgttgatcc aggtcttcca tatatttcgg cgtaatgtcg 1020

gcaatggctt cctgttcacc gccgcgcacg gcatgaacgt catctttgct gaacggcagt 1080

ttttggatgt attcagaacg agccgggctc agggcgactt ccgggttgac atgaacatca 1140

cgccattcgg tatacatatc ggacaccgag ttaaagccgc ggaaatcatc aaagccaacg 1200

ttctgcggct gcgactcttt gttttccccc atatgccatt tcccgatagc ctgggtgacg 1260

tagccctgat cgtgcagcaa ctgcggcagc gtggttaacc cttgcagacc gcccggttgc 1320

ccgtacattg gcggcatcag aatgccgtgg tggatggagt attgtccggt gagaatcgtg 1380

gcgcgggttg gggaagaact cggttgagaa tacgccgaag ttaaaatcag cccctggctg 1440

gcaacggcgt cgatatctgg tgtagggtta cccaccgcca cgccgccgcc gttaaagcca 1500

acgtccatcc agcccacatc atccagcaag aaaacaacca cgttcggttt cttaccggtt 1560

tttttctcaa gttccgccag tttctgctgg gtttctttgt cttgtgcagg atgctgcatc 1620

accggcatca tgttgtcggc aatagtggtc gccggtttaa ccagatactg gtttgggtga 1680

tcgtatccgg caaagccttt gcgtgcggtg gcggttgacg gggtatctgc tgcgctggct 1740

atgagaggaa gagctgcggc gacagcaaca acaagaagtt tgggtgaaaa cgaaaattcc 1800

atgcaaaatg ctccggtttc atgtcgtcaa aatgatgacg taattaagca ttgataattg 1860

agatccctct ccctgacagg atgattgcat aaataatagt gataaaaata aattatttat 1920

ttatccagaa atgaattgaa aaatcaggag agcattttca atcctacctc tggcgcaggt 1980

gatattgtaa ggcggtgatg ttatatcgcg ttgattattg atgctgtttt tagttttaac 2040

ggcattaata tatatgttat taattgaatg acttttatta ttcattatat atatgtgtag 2100

aattgtgcgc aggagaaata ttcactcagg aagttattac tcaggaagca aagaggatta 2160

cagaattatc tcataacaag tgttaaggga tgttatttcc cggttctctg tggcataata 2220

aacgagtaga tgctcattcc atctcttatg ttcgccttag tgcctcataa actccggaat 2280

gacgcagagc cgtttacggt gcttatcgtc cactgacaga tgtcgcttat gcctcatcag 2340

acaccatgga cacaacgttg agtgaagcac ccacttgttg tcatacagac ctgttttaac 2400

gcctgctccg taataagagc aggcgttttt ttatatatca gaaaggcccc ggaggtgctt 2460

gcctccgggt gagaaagagc tactgtggcg ggttgttctg caacgttaac atcaaaccgt 2520

cgcgacgcat cgctgcggct tcttccggct tgtgcagtct gtccagcgcg tcggcaagcc 2580

atgcgtaatc gtaggcgtcc ggacgttgtt tcagcgctgc gcggaaggcg agcgatgctt 2640

cctgccattc tccgtgtttc atcagcgact gacccagtgt gctccacaac agcgggcgat 2700

cgcccacgtt tttgatctgt tggcgcagca ctttttccaa gctgttcccg ggttaattgg 2760

gttttcagac gccggggatc ggcagcagca ggccgaatcg tcatactggc gtttcaggcc 2820

atcgatgata atttgctggg cagtatcatg atcgtcacac tcaataagat gttccgccat 2880

tgccacctgc aaggccacct gatgacgcgt tttccggctt tggtttttcc accagttacg 2940

caaaccttca ctaccgttat cggcacgcgc ctgatccatc aggccaatcc atgcctgttg 3000

ttccagcatt gcacgatgtt cttcatcacc aacatgggct ttcgccattg atgggatgat 3060

atccagcagc gaactccatg caccagtgcg gatatacgcc tgttccgcca gacgtaatac 3120

ttccggatgg cgtggcgtaa cttccagcag cttatccacg ccgtgacgtg ctgcatggtt 3180

ttcattacgg gccagttgca gacgtacacg ggtgatttct accggaatgg tgtcattgcc 3240

ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt gcttcagcat cc 3292

<210>34

<211>11165

<212>DNA

<213>大肠杆菌

<220>

<221>CDS

<222>(3791)..(4834)

<220>

<221>CDS

<222>(10459)..(10776)

<220>

<221>CDS

<222>(10134)..(10427)

<220>

<221>CDS

<222>(9836)..(10081)

<220>

<221>CDS

<222>(7816)..(9480)

<220>

<221>CDS

<222>(4878)..(7802)

<220>

<221>CDS

<222>(3460)..(3702)

<220>

<221>CDS

<222>(3054)..(3407)

<220>

<221>CDS

<222>(2613)..(3041)

<220>

<221>CDS

<222>(2198)..(2530)

<220>

<221>CDS

<222>(1939)..(2196)

<220>

<221>CDS

<222>(1573)..(1893)

<220>

<221>CDS

<222>(1102)..(1485)

<220>

<221>CDS

<222>(2)..(1099)

<400>34

c agc gat atg cag cgc ggt atc cag gct gca acg gct gca ctt cag ggc 49

Ser Asp Met Gln Arg Gly Ile Gln Ala Ala Thr Ala Ala Leu Gln Gly

1 5 10 15

ctg gtg ggc ggc aat atg gca ggc gcg ctg gca ggt gct tca gcg ccg 97

Leu Val Gly Gly Asn Met Ala Gly Ala Leu Ala Gly Ala Ser Ala Pro

20 25 30

gag ctg gcg aac atc atc ggt cat cac gcg ggt att gat gac aat aca 145

Glu Leu Ala Asn Ile Ile Gly His His Ala Gly Ile Asp Asp Asn Thr

35 40 45

gcg gca aaa gcc att gcc cat gcc att ctc ggt ggt gtg aca gca gcc 193

Ala Ala Lys Ala Ile Ala His Ala Ile Leu Gly Gly Val Thr Ala Ala

50 55 60

ctt cag ggc aac agt gcg gca gca ggc gca att ggt gcg ggt act ggt 241

Leu Gln Gly Asn Ser Ala Ala Ala Gly Ala Ile Gly Ala Gly Thr Gly

65 70 75 80

gaa gtg atc gcg tca gcc att gcg aaa agc ctc tac ccg ggc gta gat 289

Glu Val Ile Ala Ser Ala Ile Ala Lys Ser Leu Tyr Pro Gly Val Asp

85 90 95

ccg tcg aaa ctg aca gaa gat cag aag caa act gta agc acg ctg gca 337

Pro Ser Lys Leu Thr Glu Asp Gln Lys Gln Thr Val Ser Thr Leu Ala

100 105 110

acg ctg tca gcg ggt atg gcc ggc ggc att gcc agt ggc gat gtg gct 385

Thr Leu Ser Ala Gly Met Ala Gly Gly Ile Ala Ser Gly Asp Val Ala

115 120 125

ggc gcg gct gct gga gct ggt gcc ggg aag aac gtt gtt gag aat aat 433

Gly Ala Ala Ala Gly Ala Gly Ala Gly Lys Asn Val Val Glu Asn Asn

130 135 140

gcg ctg agt ctg gtt gcc aga ggc tgt gcg gtc gca gca cct tgc agg 481

Ala Leu Ser Leu Val Ala Arg Gly Cys Ala Val Ala Ala Pro Cys Arg

145 150 155 160

act aaa gtt gca gag cag ttg cta gaa atc ggg gcg aaa gcg ggc atg 529

Thr Lys Val Ala Glu Gln Leu Leu Glu Ile Gly Ala Lys Ala Gly Met

165 170 175

gcc ggg ctt gcc ggg gcg gca gtc aag gat atg gcc gac agg atg acc 577

Ala Gly Leu Ala Gly Ala Ala Val Lys Asp Met Ala Asp Arg Met Thr

180 185 190

tcc gat gaa ctg gag cat ctg att acc ctg caa atg atg ggt aat gat 625

Ser Asp Glu Leu Glu His Leu Ile Thr Leu Gln Met Met Gly Asn Asp

195 200 205

gag atc act act aag tat ctc agt tcg ttg cat gat aag tac ggt tcc 673

Glu Ile Thr Thr Lys Tyr Leu Ser Ser Leu His Asp Lys Tyr Gly Ser

210 215 220

ggg gct gcc tcg aat ccg aat atc ggt aaa gat ctg acc gat gcg gaa 721

Gly Ala Ala Ser Asn Pro Asn Ile Gly Lys Asp Leu Thr Asp Ala Glu

225 230 235 240

aaa gta gaa ctg ggc ggt tcc ggc tca gga acc ggt aca cca cca cca 769

Lys Val Glu Leu Gly Gly Ser Gly Ser Gly Thr Gly Thr Pro Pro Pro

245 250 255

tcg gaa aat gat cct aag cag caa aat gaa aaa act gta gat aag ctt 817

Ser Glu Asn Asp Pro Lys Gln Gln Asn Glu Lys Thr Val Asp Lys Leu

260 265 270

aat cag aag caa gaa agt gcg att aag aag atc gat aac act ata aaa 865

Asn Gln Lys Gln Glu Ser Ala Ile Lys Lys Ile Asp Asn Thr Ile Lys

275 280 285

aat gct ctg aaa gat cat gat att att gga act ctc aag gat atg gat 913

Asn Ala Leu Lys Asp His Asp Ile Ile Gly Thr Leu Lys Asp Met Asp

290 295 300

ggt aag cca gtt cct aaa gag aat gga gga tat tgg gat cat atg cag 961

Gly Lys Pro Val Pro Lys Glu Asn Gly Gly Tyr Trp Asp His Met Gln

305 310 315 320

gaa atg caa aat acg ctc aga gga tta aga aat cat gcg gat acg ttg 1009

Glu Met Gln Asn Thr Leu Arg Gly Leu Arg Asn His Ala Asp Thr Leu

325 330 335

aaa aac gtc aac aat cct gaa gct cag gct gcg tat ggc aga gca aca 1057

Lys Asn Val Asn Asn Pro Glu Ala Gln Ala Ala Tyr Gly Arg Ala Thr

340 345 350

gat gct att aat aaa ata gaa tca gcc ttg aaa gga tat gga at atg 1104

Asp Ala Ile Asn Lys Ile Glu Ser Ala Leu Lys Gly Tyr Gly Met

355 360 365

att acc tta cgt aaa ttg att gga aac atc aat atg aca aaa gag cct 1152

Ile Thr Leu Arg Lys Leu Ile Gly Asn Ile Asn Met Thr Lys Glu Pro

370 375 380

gag caa caa tca ccg ctt gaa ctc tgg ttc gaa cgt atc ata gat gtg 1200

Glu Gln Gln Ser Pro Leu Glu Leu Trp Phe Glu Arg Ile Ile Asp Val

385 390 395

cct ctt gaa aag tta aca gtg gaa gat ctt tgc cgc gct atc cga caa 1248

Pro Leu Glu Lys Leu Thr Val Glu Asp Leu Cys Arg Ala Ile Arg Gln

400 405 410 415

aat tta tgt att gat cag ttg atg cca aga gtg ttg gaa gtt cta act 1296

Asn Leu Cys Ile Asp Gln Leu Met Pro Arg Val Leu Glu Val Leu Thr

420 425 430

aaa gag ccg tta gcg ggt gaa tat tac gat ggt gaa cta att gca gct 1344

Lys Glu Pro Leu Ala Gly Glu Tyr Tyr Asp Gly Glu Leu Ile Ala Ala

435 440 445

tta tca acg ata aaa gga gaa gat cta aaa gat cag aaa agt acc ttt 1392

Leu Ser Thr Ile Lys Gly Glu Asp Leu Lys Asp Gln Lys Ser Thr Phe

450 455 460

acc caa ata agg caa ctt ata aac cag cta gaa ccg tca gat att aac 1440

Thr Gln Ile Arg Gln Leu Ile Asn Gln Leu Glu Pro Ser Asp Ile Asn

465 470 475

gat gat tta aga aaa gat ata tta aaa atc aat cag ata att gta 1485

Asp Asp Leu Arg Lys Asp Ile Leu Lys Ile Asn Gln Ile Ile Val

480 485 490

taactaatcc cggccactga gccgagatct tctttgtgtg ccgggcatgt tcagcagctt 1545

gggggtgaaa gtcccctgtc cagcctg atg gtg gcg aag gcg ttc gcg tac gca 1599

Met Val Ala Lys Ala Phe Ala Tyr Ala

495 500

ctt aac cag tgg ccg gca ctg acg tac tat gcg aac gat ggc tgg gtg 1647

Leu Asn Gln Trp Pro Ala Leu Thr Tyr Tyr Ala Asn Asp Gly Trp Val

505 510 515

gaa atc gac aac aac atc gct gaa aat gcc ctg cgg gcg gtc agt ctg 1695

Glu Ile Asp Asn Asn Ile Ala Glu Asn Ala Leu Arg Ala Val Ser Leu

520 525 530 535

ggt cgt aaa aac ttc ctg ttc ttc ggc tct gac cat ggt ggt gag cgg 1743

Gly Arg Lys Asn Phe Leu Phe Phe Gly Ser Asp His Gly Gly Glu Arg

540 545 550

gga gcg cta ctg tac agc ctg atc ggg acg tgc aaa ctg aat gac gtg 1791

Gly Ala Leu Leu Tyr Ser Leu Ile Gly Thr Cys Lys Leu Asn Asp Val

555 560 565

gat cca gaa agc tac ctt cgc cat gtg ctt gcc gtc ata gca gac tgg 1839

Asp Pro Glu Ser Tyr Leu Arg His Val Leu Ala Val Ile Ala Asp Trp

570 575 580

ccg gtc aac cgg gtc agc gaa ctg ctt ccg tgg cgc ata gca ctg cca 1887

Pro Val Asn Arg Val Ser Glu Leu Leu Pro Trp Arg Ile Ala Leu Pro

585 590 595

gct gaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac agaaa atg ctg 1944

Ala Glu Met Leu

600

atg tct gta cag aaa gaa aag aac gtc gca gag agt gtg gta tct gaa 1992

Met Ser Val Gln Lys Glu Lys Asn Val Ala Glu Ser Val Val Ser Glu

605 610 615

acg cat acc ggc gac agc gta tat gct tcc ctg ttt gaa aaa att aac 2040

Thr His Thr Gly Asp Ser Val Tyr Ala Ser Leu Phe Glu Lys Ile Asn

620 625 630 635

ctg aat ccg gta tct gcc ctg agt gca ctg gat aac cct ttc cgg tca 2088

Leu Asn Pro Val Ser Ala Leu Ser Ala Leu Asp Asn Pro Phe Arg Ser

640 645 650

gca gat aac gcg act ggc aga att acc tcc agc ata caa cct gcg gtg 2136

Ala Asp Asn Ala Thr Gly Arg Ile Thr Ser Ser Ile Gln Pro Ala Val

655 660 665

cag tgc gca gct gct gca gca act gag ggt tct tgt ccc cgg caa tcc 2184

Gln Cys Ala Ala Ala Ala Ala Thr Glu Gly Ser Cys Pro Arg Gln Ser

670 675 680

ccg tgt tca gga a atg gtg gat aac tgg cag aag agt gta agg agt cgt 2233

Pro Cys Ser Gly Met Val Asp Asn Trp Gln Lys Ser Val Arg Ser Arg

685 690 695

gcg ctc ccg gaa gag gcg atg acg ggc tgg aac gaa ggc atg atc cgc 2281

Ala Leu Pro Glu Glu Ala Met Thr Gly Trp Asn Glu Gly Met Ile Arg

700 705 710 715

tta cag cag ttg gct gag cgc ctg aac cgt cag gat gaa cag cgg gga 2329

Leu Gln Gln Leu Ala Glu Arg Leu Asn Arg Gln Asp Glu Gln Arg Gly

720 725 730

aaa tac atg acg gtc agt gaa ctg aaa acg gag gtg ttt ggc atc atg 2377

Lys Tyr Met Thr Val Ser Glu Leu Lys Thr Glu Val Phe Gly Ile Met

735 740 745

cag gct ttt aac cgg cat atc ccg gcg gaa gag cag tta cgt cgc tac 2425

Gln Ala Phe Asn Arg His Ile Pro Ala Glu Glu Gln Leu Arg Arg Tyr

750 755 760

ggt gaa gtc cgt aac cag aat ggc agt gaa cag cag caa aaa cag gct 2473

Gly Glu Val Arg Asn Gln Asn Gly Ser Glu Gln Gln Gln Lys Gln Ala

765 770 775

gaa atg gcg cta aat cag tta att aac cgt tat cag atg ata cgt gca 2521

Glu Met Ala Leu Asn Gln Leu Ile Asn Arg Tyr Gln Met Ile Arg Ala

780 785 790 795

ggc aaa caa tagtggtagc cataatgcag gagcaaagcc tgaatcagga 2570

Gly Lys Gln

agagttattc tgactgagtt tggttttctg gcgattcttg tg atg gtg gga tgt 2624

Met Val Gly Cys

800

gct tgg tta gct gaa cag gcc ttt tcc gac cat gcg ctt tca cca cac 2672

Ala Trp Leu Ala Glu Gln Ala Phe Ser Asp His Ala Leu Ser Pro His

805 810 815

agt gct tgg ccg tac agt gca tcg cgc gat gcc ggg ctg gcc gat acg 2720

Ser Ala Trp Pro Tyr Ser Ala Ser Arg Asp Ala Gly Leu Ala Asp Thr

820 825 830

ggc gcg ggc ggc tat ccc act tgt aaa cag cgg tgg gcc gac gac acc 2768

Gly Ala Gly Gly Tyr Pro Thr Cys Lys Gln Arg Trp Ala Asp Asp Thr

835 840 845 850

gtt ggg ctg aaa gcc cgt cta ctg caa ctt cct gcc cta gat atc tgg 2816

Val Gly Leu Lys Ala Arg Leu Leu Gln Leu Pro Ala Leu Asp Ile Trp

855 860 865

acg gcg ttt aaa aaa atc gac cag tcg cag gta gtg tat gaa gag gcc 2864

Thr Ala Phe Lys Lys Ile Asp Gln Ser Gln Val Val Tyr Glu Glu Ala

870 875 880

gtg ctg cgc tcg cgg gtc agt gaa cga aat atg cag gta tcg cag aat 2912

Val Leu Arg Ser Arg Val Ser Glu Arg Asn Met Gln Val Ser Gln Asn

885 890 895

ggg cgc gtt tat cca agc tat ggc ggt aac gtt gat ggc acc gtc gcc 2960

Gly Arg Val Tyr Pro Ser Tyr Gly Gly Asn Val Asp Gly Thr Val Ala

900 905 910

aat gcc gcc acc cgg ttg gca tcc ggc gct aga aat atc ctc ggc agc 3008

Asn Ala Ala Thr Arg Leu Ala Ser Gly Ala Arg Asn Ile Leu Gly Ser

915 920 925 930

ata gcg gca tgt acg gca ttc gac agc gtg cgt taggcactac cg atg gta 3059

Ile Ala Ala Cys Thr Ala Phe Asp Ser Val Arg Met Val

935 940

cag gcg cag ctg caa ata gcg ctg gtg atc tgt att ccg ctg ata acg 3107

Gln Ala Gln Leu Gln Ile Ala Leu Val Ile Cys Ile Pro Leu Ile Thr

945 950 955

ctc tgt tcg gcg tgg gat gtg aaa gta gtg atg acg ctg acg ttt gtg 3155

Leu Cys Ser Ala Trp Asp Val Lys Val Val Met Thr Leu Thr Phe Val

960 965 970 975

cag ttt gca cta ttt ttc ctc acc ttt tgg tgg gaa ctg gca cgg tgg 3203

Gln Phe Ala Leu Phe Phe Leu Thr Phe Trp Trp Glu Leu Ala Arg Trp

980 985 990

ctt gat agc tgg ctg ctg gat gtg ctc tac aac agc gat acc cac agt 3251

Leu Asp Ser Trp Leu Leu Asp Val Leu Tyr Asn Ser Asp Thr His Ser

995 1000 1005

agc tgg aat tta gcc ggg atc cag aat acg cag gat gac gtg att atc 3299

Ser Trp Asn Leu Ala Gly Ile Gln Asn Thr Gln Asp Asp Val Ile Ile

1010 1015 1020

aat ctg gtg atg agg ttg atg ttt ctg gtg ttg ccg aca ttc tgg ctg 3347

Asn Leu Val Met Arg Leu Met Phe Leu Val Leu Pro Thr Phe Trp Leu

1025 1030 1035

ggg gcg atg acg tgg gct gga gtg agg gtt ggc gtg gcg ctg aat gga 3395

Gly Ala Met Thr Trp Ala Gly Val Arg Val Gly Val Ala Leu Asn Gly

1040 1045 1050 1055

gcg ctg gcg gga tgattgggag gtgattcgcc aatctcactt tcctatacac 3447

Ala Leu Ala Gly

atataaaatg ta atg aaa tat ctc ttt ttt gag aat ata cat tct ata ttt 3498

Met Lys Tyr Leu Phe Phe Glu Asn Ile His Ser Ile Phe

1060 1065 1070

tta aca ttc agt ctc ttc cga aca tct gtg tcg cct gat ttc cca atg 3546

Leu Thr Phe Ser Leu Phe Arg Thr Ser Val Ser Pro Asp Phe Pro Met

1075 1080 1085

att ttt gca ttg ccc tca atc att tta ggt caa ttt acg acc aac caa 3594

Ile Phe Ala Leu Pro Ser Ile Ile Leu Gly Gln Phe Thr Thr Asn Gln

1090 1095 1100

tta act aac ttt gtg ata tgt atg ggt aac acc gtt gaa cgt cgg ctg 3642

Leu Thr Asn Phe Val Ile Cys Met Gly Asn Thr Val Glu Arg Arg Leu

1105 1110 1115 1120

ggt gtt gtt cat aat ccc ttt aaa agg tct ggg gat ggc cat gac ctc 3690

Gly Val Val His Asn Pro Phe Lys Arg Ser Gly Asp Gly His Asp Leu

1125 1130 1135

agg gcg gta gcg tgaccaaagt tcatatccat accaattatt tttatttaaa 3742

Arg Ala Val Ala

1140

atatcaactt attcgagttg ttttatttag ttcaaagaag gtatcaaa ttg ata gtt 3799

Leu Ile Val

ata gat ttt ttt tgt ggc tgt ggt gga gcc agt gaa ggg cta cgt cag 3847

Ile Asp Phe Phe Cys Gly Cys Gly Gly Ala Ser Glu Gly Leu Arg Gln

1145 1150 1155

gct ggc ttt gat atc gag ctt gga tta gat att gac caa caa gca tca 3895

Ala Gly Phe Asp Ile Glu Leu Gly Leu Asp Ile Asp Gln Gln Ala Ser

1160 1165 1170 1175

gaa aca ttt aaa gct aat ttc cct gat gca aaa ttc atc caa gat gat 3943

Glu Thr Phe Lys Ala Asn Phe Pro Asp Ala Lys Phe Ile Gln Asp Asp

1180 1185 1190

att agg aaa atc gaa cct caa gat atc tcc gac atc att gat att aaa 3991

Ile Arg Lys Ile Glu Pro Gln Asp Ile Ser Asp Ile Ile Asp Ile Lys

1195 1200 1205

gct aaa cgg cct ttg tta ctg agt gca tgt gca cca tgt caa cca ttt 4039

Ala Lys Arg Pro Leu Leu Leu Ser Ala Cys Ala Pro Cys Gln Pro Phe

1210 1215 1220

tcg caa cag aat aaa aat aaa act agt gac gac tca agg aga aat cta 4087

Ser Gln Gln Asn Lys Asn Lys Thr Ser Asp Asp Ser Arg Arg Asn Leu

1225 1230 1235

cta aat gaa act cat cgt ttt att aga gaa ctt ctt cct gaa tat att 4135

Leu Asn Glu Thr His Arg Phe Ile Arg Glu Leu Leu Pro Glu Tyr Ile

1240 1245 1250 1255

atg ctt gaa aat gtt cct gga atg caa aaa att gat gaa gaa aaa gaa 4183

Met Leu Glu Asn Val Pro Gly Met Gln Lys Ile Asp Glu Glu Lys Glu

1260 1265 1270

ggc cca ttt cag gag ttt att aag cta ctt aaa gag tta gag tat aac 4231

Gly Pro Phe Gln Glu Phe Ile Lys Leu Leu Lys Glu Leu Glu Tyr Asn

1275 1280 1285

tat ata tct ttt ata gcc aat gct gag aac tat ggg att ccc caa aga 4279

Tyr Ile Ser Phe Ile Ala Asn Ala Glu Asn Tyr Gly Ile Pro Gln Arg

1290 1295 1300

aga aaa aga ctc gtg ctc tta gct agt cga gta ggt aaa gtt acc cta 4327

Arg Lys Arg Leu Val Leu Leu Ala Ser Arg Val Gly Lys Val Thr Leu

1305 1310 1315

cca gag ata acc cat ggt aaa aat aaa atc cca ttc aaa act gta cga 4375

Pro Glu lle Thr His Gly Lys Asn Lys lle Pro Phe Lys Thr Val Arg

1320 1325 1330 1335

gat tat atc cag gac ttc aca aag tta tgt tca gga gaa acc gac ccc 4423

Asp Tyr Ile Gln Asp Phe Thr Lys Leu Cys Ser Gly Glu Thr Asp Pro

1340 1345 1350

aaa gat cct tta cat agg gct gga aca ctg agc cct ctt aac cta aaa 4471

Lys Asp Pro Leu His Arg Ala Gly Thr Leu Ser Pro Leu Asn Leu Lys

1355 1360 1365

aga att atg cac act cca gaa gga ggg gat aga aga aat tgg cca gaa 4519

Arg Ile Met His Thr Pro Glu Gly Gly Asp Arg Arg Asn Trp Pro Glu

1370 1375 1380

gag tta gtt aat aaa tgc cat aaa aat tat gat ggc cac aca gat act 4567

Glu Leu Val Asn Lys Cys His Lys Asn Tyr Asp Gly His Thr Asp Thr

1385 1390 1395

tat gga aga atg agt tgg gat aag cct gcg cct aca ctt acg acg aaa 4615

Tyr Gly Arg Met Ser Trp Asp Lys Pro Ala Pro Thr Leu Thr Thr Lys

1400 1405 1410 1415

tgt aat agt tac tcc aat ggt cgt ttt ggg cat cct gac ccc act caa 4663

Cys Asn Ser Tyr Ser Asn Gly Arg Phe Gly His Pro Asp Pro Thr Gln

1420 1425 1430

cat aga gca att agc ata aga gaa gca tca aga tta caa aca ttt cct 4711

His Arg Ala Ile Ser Ile Arg Glu Ala Ser Arg Leu Gln Thr Phe Pro

1435 1440 1445

tta agc tat gtt ttt aaa ggt tcg ctg aat tca atg gca aag caa atc 4759

Leu Ser Tyr Val Phe Lys Gly Ser Leu Asn Ser Met Ala Lys Gln Ile

1450 1455 1460

ggc aat gct gta cct tgc gaa ctc gct aga cta ttt ggg cta cat ctc 4807

Gly Asn Ala Val Pro Cys Glu Leu Ala Arg Leu Phe Gly Leu His Leu

1465 1470 1475

ata gaa aat tgt act aat aag gat tca tagatatatg gctaaaataa 4854

Ile Glu Asn cys Thr Asn Lys Asp Ser

1480 1485

gaacaaaggc tcgagctttg gac atg ctt ggc aga caa caa att gca ggt ata 4907

Met Leu Gly Arg Gln Gln Ile Ala Gly Ile

1490 1495

cct act gcc ttg agt gag tta ttt aaa aat gct cat gat gcc tat gct 4955

Pro Thr Ala Leu Ser Glu Leu Phe Lys Asn Ala His Asp Ala Tyr Ala

1500 1505 1510

gat aat gtc gaa gtt gat ttt ttt agg aaa gaa aat ctt ctt atc ttg 5003

Asp Asn Val Glu Val Asp Phe Phe Arg Lys Glu Asn Leu Leu Ile Leu

1515 1520 1525 1530

aga gat gat gga tta ggt atg aca acc gat gaa ttt gaa gag agg tgg 5051

Arg Asp Asp Gly Leu Gly Met Thr Thr Asp Glu Phe Glu Glu Arg Trp

1535 1540 1545

ttg act att gga acc tcc agc aaa tta atc gac gat gat gca att aat 5099

Leu Thr Ile Gly Thr Ser Ser Lys Leu Ile Asp Asp Asp Ala Ile Asn

1550 1555 1560

aaa cca gca gtg gat agt aat aaa gcc ttt cgc cct atc atg gga gag 5147

Lys Pro Ala Val Asp Ser Asn Lys Ala Phe Arg Pro Ile Met Gly Glu

1565 1570 1575

aaa gga ata ggc cgt tta tct atc gca gca att gga cca cag gtg ctg 5195

Lys Gly Ile Gly Arg Leu Set Ile Ala Ala Ile Gly Pro Gln Val Leu

1580 1585 1590

gtt ctt act agg gcc aaa aga gac aat gag ctt aag cca tta gtt gct 5243

Val Leu Thr Arg Ala Lys Arg Asp Asn Glu Leu Lys Pro Leu Val Ala

1595 1600 1605 1610

gca ttt gtt aat tgg agt tta ttt gct ata cca tca ctt gat ctt gat 5291

Ala Phe Val Asn Trp Ser Leu Phe Ala Ile Pro Ser Leu Asp Leu Asp

1615 1620 1625

gat ata gaa ata cca att aga act att atc aac gac gaa tgc ttc act 5339

Asp Ile Glu Ile Pro Ile Arg Thr Ile Ile Asn Asp Glu Cys Phe Thr

1630 1635 1640

aaa aaa act ctt gat gag atg att gag caa gca aga aat aat tta gac 5387

Lys Lys Thr Leu Asp Glu Met Ile Glu Gln Ala Arg Asn Asn Leu Asp

1645 1650 1655

tct tta tca cac aaa ata tca aaa tca aaa gta tca caa ata aat aca 5435

Ser Leu Ser His Lys Ile Ser Lys Ser Lys Val Ser Gln Ile Asn Thr

1660 1665 1670

caa tta tca tct ttt gaa ttt gat cct att cta tgg gaa aaa aaa tta 5483

Gln Leu Ser Ser Phe Glu Phe Asp Pro Ile Leu Trp Glu Lys Lys Leu

1675 1680 1685 1690

ggt ggg cta aga cta tct gga gat ggg cat gga act cac ttc ata ata 5531

Gly Gly Leu Arg Leu Ser Gly Asp Gly His Gly Thr His Phe Ile Ile

1695 1700 1705

atg cct acc gaa gaa ata tta ata gat gac att tcc acg agc gat agc 5579

Met Pro Thr Glu Glu Ile Leu Ile Asp Asp Ile Ser Thr Ser Asp Ser

1710 1715 1720

aat aaa aca tca gag cag tct tct cgc tta gaa aaa gct tta tta ggt 5627

Asn Lys Thr Ser Glu Gln Ser Ser Arg Leu Glu Lys Ala Leu Leu Gly

1725 1730 1735

ttt aca aac aca atg tac agt gat tca aac cct cct att ata gct cgt 5675

Phe Thr Asn Thr Met Tyr Ser Asp Ser Asn Pro Pro Ile Ile Ala Arg

1740 1745 1750

ttt aga gac tat ctg gaa gat ggt gag tgc att gac aga att agc gaa 5723

Phe Arg Asp Tyr Leu Glu Asp Gly Glu Cys Ile Asp Arg Ile Ser Glu

1755 1760 1765 1770

tca att ttt ttt aca ccg caa gaa ttc aat ctt gca gat cac cac att 5771

Ser Ile Phe Phe Thr Pro Gln Glu Phe Asn Leu Ala Asp His His Ile

1775 1780 1785

gaa gga tgg ttc aat gaa ttt ggt caa ttc agt gga act gtt tct gtt 5819

Glu Gly Trp Phe Asn Glu Phe Gly Gln Phe Set Gly Thr Val Ser Val

1790 1795 1800

tat ggt gaa gag cca att cat cat gtc gtg act tgg aaa aat aat aat 5867

Tyr Gly Glu Glu Pro Ile His His Val Val Thr Trp Lys Asn Asn Asn

1805 1810 1815

caa tta acc caa tgc ggt cca ttt aaa ata aaa tta gcg tat att cat 5915

Gln Leu Thr Gln Cys Gly Pro Phe Lys Ile Lys Leu Ala Tyr Ile His

1820 1825 1830

ggt cgg ctt cgt gat tca cgc tta ccc atg gag ttg tgg gcc cct ctg 5963

Gly Arg Leu Arg Asp Ser Arg Leu Pro Met Glu Leu Trp Ala Pro Leu

1835 1840 1845 1850

aag gag aaa aca gat aga tat ggt ggt tta tat atc tat cga gat gga 6011

Lys Glu Lys Thr Asp Arg Tyr Gly Gly Leu Tyr Ile Tyr Arg Asp Gly

1855 1860 1865

tta aga att ttg ccc tat gga gat tca gat acg gat ttt cta aaa ata 6059

Leu Arg Ile Leu Pro Tyr Gly Asp Ser Asp Thr Asp Phe Leu Lys Ile

1870 1875 1880

gaa aag aga aga acg tta tcc gct tct gaa tat ttt ttc tca tat cga 6107

Glu Lys Arg Arg Thr Leu Ser Ala Ser Glu Tyr Phe Phe Ser Tyr Arg

1885 1890 1895

cgt ttg ttt gga gca ata gaa tta aca aaa gaa aac aat gct tca tta 6155

Arg Leu Phe Gly Ala Ile Glu Leu Thr Lys Glu Asn Asn Ala Ser Leu

1900 1905 1910

gtt gaa aaa gct ggg cga gaa gga ttc att gaa aat aag cca tat aaa 6203

Val Glu Lys Ala Gly Arg Glu Gly Phe Ile Glu Asn Lys Pro Tyr Lys

1915 1920 1925 1930

cag ttt aaa gaa atg ctt gaa aat ttc ttc atc gaa atc gca aga gat 6251

Gln Phe Lys Glu Met Leu Glu Asn Phe Phe Ile Glu Ile Ala Arg Asp

1935 1940 1945

ttc ttt aag gac gat ggc gat atg tct gaa tta ttt gtt gag aca aag 6299

Phe Phe Lys Asp Asp Gly Asp Met Ser Glu Leu Phe Val Glu Thr Lys

1950 1955 1960

caa cgt aga aat gaa gaa cat gat ttg tta tct aaa aga tct aaa caa 6347

Gln Arg Arg Asn Glu Glu His Asp Leu Leu Ser Lys Arg Ser Lys Gln

1965 1970 1975

act aaa gct aaa aaa gat aga tta aag aaa gat ctg tat gat ttt ttt 6395

Thr Lys Ala Lys Lys Asp Arg Leu Lys Lys Asp Leu Tyr Asp Phe Phe

1980 1985 1990

gat aag tta gat aat gat tac tgg aat att gaa ata aat aag cta atc 6443

Asp Lys Leu Asp Asn Asp Tyr Trp Asn Ile Glu Ile Asn Lys Leu Ile

1995 2000 2005 2010

aat aaa aac gag gaa tat ttc tcc agt aca gaa ata aca gac acc aat 6491

Asn Lys Asn Glu Glu Tyr Phe Ser Ser Thr Glu Ile Thr Asp Thr Asn

2015 2020 2025

ata gat tat gta tac aat aaa att aaa gaa caa aat gat gct atc att 6539

Ile Asp Tyr Val Tyr Asn Lys Ile Lys Glu Gln Asn Asp Ala Ile Ile

2030 2035 2040

aaa aat cta cgt aat tct gtg gat ata aag aaa ccc tct gga gtt gga 6587

Lys Asn Leu Arg Asn Ser Val Asp Ile Lys Lys Pro Ser Gly Val Gly

2045 2050 2055

tta aca aaa gag tta tct aat tta tgg gat aga tat caa ata gaa aga 6635

Leu Thr Lys Glu Leu Ser Asn Leu Trp Asp Arg Tyr Gln Ile Glu Arg

2060 2065 2070

caa aaa ata ctg tta tca cta aat gag cta aaa gat aac gtt gat aga 6683

Gln Lys Ile Leu Leu Ser Leu Asn Glu Leu Lys Asp Asn Val Asp Arg

2075 2080 2085 2090

aag ctt ata gaa ctg gat aat aaa aat aat gat ttt ctc aac tta cgg 6731

Lys Leu Ile Glu Leu Asp Asn Lys Asn Asn Asp Phe Leu Asn Leu Arg

2095 2100 2105

aag aga ctt gaa gat tct ttg aat cta caa caa agt tac tat gaa aaa 6779

Lys Arg Leu Glu Asp Ser Leu Asn Leu Gln Gln Ser Tyr Tyr Glu Lys

2110 2115 2120

gaa cta aca aag tta tat aat gac gct aaa aat gct ttg aaa gat gtg 6827

Glu Leu Thr Lys Leu Tyr Asn Asp Ala Lys Asn Ala Leu Lys Asp Val

2125 2130 2135

caa tct aaa gca aat agg tta att tct gat aat aag aaa aaa cat aag 6875

Gln Ser Lys Ala Asn Arg Leu Ile Ser Asp Asn Lys Lys Lys His Lys

2140 2145 2150

agt gaa cta aaa aac att tct tat gaa ttc caa tca act aat ctc aat 6923

Ser Glu Leu Lys Asn Ile Ser Tyr Glu Phe Gln Ser Thr Asn Leu Asn

2155 2160 2165 2170

ggc aaa gat act gcg tat ata ttg gat gta aaa aga aat cta gaa agt 6971

Gly Lys Asp Thr Ala Tyr Ile Leu Asp Val Lys Arg Asn Leu Glu Ser

2175 2180 2185

aaa att gag aat act tca aac gaa gtg att aat gaa ata aga aaa cta 7019

Lys Ile Glu Asn Thr Ser Asn Glu Val Ile Asn Glu Ile Arg Lys Leu

2190 2195 2200

acc gac cag att gca ata att agt gat agt acc act tct gaa aat tta 7067

Thr Asp Gln Ile Ala Ile Ile Ser Asp Ser Thr Thr Ser Glu Asn Leu

2205 2210 2215

tca tcg gct caa gta act gaa gca atc gaa act gaa ctt gaa cat tta 7115

Ser Ser Ala Gln Val Thr Glu Ala Ile Glu Thr Glu Leu Glu His Leu

2220 2225 2230

cga gac caa caa gca aat aac gca gag tta ata cta ctt ggc atg gct 7163

Arg Asp Gln Gln Ala Asn Asn Ala Glu Leu Ile Leu Leu Gly Met Ala

2235 2240 2245 2250

ctt tct gta gta cat cat gaa ttt aat ggt aat att agg gca att aga 7211

Leu Ser Val Val His His Glu Phe Asn Gly Asn Ile Arg Ala Ile Arg

2255 2260 2265

agt gcg cta agg gaa tta aaa gca tgg gct gac aga aat cct aag ctt 7259

Ser Ala Leu Arg Glu Leu Lys Ala Trp Ala Asp Arg Asn Pro Lys Leu

2270 2275 2280

gat att ata tac caa aaa atc aga act agt ttt gat cac tta gat ggt 7307

Asp Ile Ile Tyr Gln Lys Ile Arg Thr Ser Phe Asp His Leu Asp Gly

2285 2290 2295

tat tta aaa acc ttt aca cca ttg aca aga cgt tta agt cgc tct aaa 7355

Tyr Leu Lys Thr Phe Thr Pro Leu Thr Arg Arg Leu Ser Arg Ser Lys

2300 2305 2310

acc aat ata act gga act gcc att tta gaa ttt atc aga gat gta ttc 7403

Thr Asn Ile Thr Gly Thr Ala Ile Leu Glu Phe Ile Arg Asp Val Phe

2315 2320 2325 2330

gat gat cgt ctt gag aaa gaa gga att gaa tta ttc act acc tca aag 7451

Asp Asp Arg Leu Glu Lys Glu Gly Ile Glu Leu Phe Thr Thr Ser Lys

2335 2340 2345

ttt gtt aat caa gaa att gta act tac aca tca acc att tac cct gtc 7499

Phe Val Asn Gln Glu Ile Val Thr Tyr Thr Ser Thr Ile Tyr Pro Val

2350 2355 2360

ttt ata aat cta att gat aac gca ata tac tgg ctt ggg aaa aca act 7547

Phe Ile Asn Leu Ile Asp Asn Ala Ile Tyr Trp Leu Gly Lys Thr Thr

2365 2370 2375

gga gaa aaa aga ctt ata ctt gat gct act gaa aca gga ttt gtt att 7595

Gly Glu Lys Arg Leu Ile Leu Asp Ala Thr Glu Thr Gly Phe Val Ile

2380 2385 2390

ggt gat act ggt ccc ggt gtt tca act aga gat cga gat ata ata ttt 7643

Gly Asp Thr Gly Pro Gly Val Ser Thr Arg Asp Arg Asp Ile Ile Phe

2395 2400 2405 2410

gat atg gga ttt aca cga aaa aca gga ggg cgt gga atg gga tta ttc 7691

Asp Met Gly Phe Thr Arg Lys Thr Gly Gly Arg Gly Met Gly Leu Phe

2415 2420 2425

att tcc aaa gag tgt tta tct cga gat gga ttt act ata aga ttg gat 7739

Ile Ser Lys Glu Cys Leu Ser Arg Asp Gly Phe Thr Ile Arg Leu Asp

2430 2435 2440

gat tac act cct gaa cag ggt gct ttc ttt att att gag cca tca gaa 7787

Asp Tyr Thr Pro Glu Gln Gly Ala Phe Phe Ile Ile Glu Pro Ser Glu

2445 2450 2455

gaa aca agt gaa tag cggatataaa taa atg aca agc tct act gat ttt 7836

Glu Thr Ser Glu Met Thr Ser Ser Thr Asp Phe

2460 2465 2470

cat aaa ctt tct gaa gac tgc gtt cgc cgt ttt tta cat tct gta gtt 7884

His Lys Leu Ser Glu Asp Cys Val Arg Arg Phe Leu His Ser Val Val

2475 2480 2485

gct gta gat gac aat atg tct ttt gga gct ggt agt gat act ttc cct 7932

Ala Val Asp Asp Asn Met Ser Phe Gly Ala Gly Ser Asp Thr Phe Pro

2490 2495 2500

aca gac gaa gat att aat gct tta gtt gat ccc gac gat gat cct aca 7980

Thr Asp Glu Asp Ile Asn Ala Leu Val Asp Pro Asp Asp Asp Pro Thr

2505 2510 2515

cca ata ata aca gca tca gca tcc cca agg ata gaa tca act aaa tca 8028

Pro Ile Ile Thr Ala Ser Ala Ser Pro Arg Ile Glu Ser Thr Lys Ser

2520 2525 2530

aaa gca aag gta aaa aac cat cct ttt gat tac caa gct cta gca gaa 8076

Lys Ala Lys Val Lys Asn His Pro Phe Asp Tyr Gln Ala Leu Ala Glu

2535 2540 2545 2550

gct ttc gcc aaa gat ggt att gct tgt tgc gga tta tta gct aag agt 8124

Ala Phe Ala Lys Asp Gly Ile Ala Cys Cys Gly Leu Leu Ala Lys Ser

2555 2560 2565

ttt aat gtt gaa gaa aga gat ata att aca gca tca tcc cac aag gca 8172

Phe Asn Val Glu Glu Arg Asp Ile Ile Thr Ala Ser Ser His Lys Ala

2570 2575 2580

gat ata aca ata ctt gac tgg gat atg caa agc gat agt ggg caa ttt 8220

Asp Ile Thr Ile Leu Asp Trp Asp Met Gln Ser Asp Ser Gly Gln Phe

2585 2590 2595

gct att gaa ata ata aaa tcg ata atc gtt tca gat ata aat tct gga 8268

Ala Ile Glu Ile Ile Lys Ser Ile Ile Val Ser Asp Ile Asn Ser Gly

2600 2605 2610

gga cgt tta cgt ctt ctt tct att tat act ggt gaa cat gtt act gct 8316

Gly Arg Leu Arg Leu Leu Ser Ile Tyr Thr Gly Glu His Val Thr Ala

2615 2620 2625 2630

gtt ata act aag ttg aac aat gag tta aag aaa aca tac cgt agc gta 8364

Val Ile Thr Lys Leu Asn Asn Glu Leu Lys Lys Thr Tyr Arg Ser Val

2635 2640 2645

ata aaa aat gat gat agt att ttt att gaa gat aac tat gca ctc gaa 8412

Ile Lys Asn Asp Asp Ser Ile Phe Ile Glu Asp Asn Tyr Ala Leu Glu

2650 2655 2660

caa tgg tgt ata gtt gtt att agt aaa gac gtt tat gaa aaa gat ctt 8460

Gln Trp Cys Ile Val Val Ile Ser Lys Asp Val Tyr Glu Lys Asp Leu

2665 2670 2675

cca aat gtg tta ata aaa aaa ttc act aac ctt aca gct ggg ttg cta 8508

Pro Asn Val Leu Ile Lys Lys Phe Thr Asn Leu Thr Ala Gly Leu Leu

2680 2685 2690

tcc aac gcc gca ctc tct tgc att tct gaa ata aga gaa aaa acc cat 8556

ser Asn Ala Ala Leu Ser Cys Ile Ser Glu Ile Arg Glu Lys Thr His

2695 2700 2705 2710

ggg ata tta aca aaa tat aat aat aaa tta gac act gca tat gtt tcc 8604

Gly Ile Leu Thr Lys Tyr Asn Asn Lys Leu Asp Thr Ala Tyr Val Ser

2715 2720 2725

cac atc tta aat tta ata aaa tcc aag gag tca agg gca tat gct tat 8652

His Ile Leu Asn Leu Ile Lys Ser Lys Glu Ser Arg Ala Tyr Ala Tyr

2730 2735 2740

gaa aat gct cat gat tat gca gta gat tta att tct gaa gaa ata aga 8700

Glu Asn Ala His Asp Tyr Ala Val Asp Leu Ile Ser Glu Glu Ile Arg

2745 2750 2755

tca ata ttg caa ata agt gaa aac tta aag aaa tct cta agc aaa aac 8748

Ser Ile Leu Gln Ile Ser Glu Asn Leu Lys Lys Ser Leu Ser Lys Asn

2760 2765 2770

tcc tta tcc cat tgg cct att ttt cac tat gca aaa aat ggt tgt aag 8796

Ser Leu Ser His Trp Pro Ile Phe His Tyr Ala Lys Asn Gly Cys Lys

2775 2780 2785 2790

aat ttt cta tta act gga aaa aaa caa aaa gac tta tca gta gaa cat 8844

Asn Phe Leu Leu Thr Gly Lys Lys Gln Lys Asp Leu Ser Val Glu His

2795 2800 2805

cta agg aat ata ctc tct gct gat tct tta gaa gaa att caa cac gct 8892

Leu Arg Asn Ile Leu Ser Ala Asp Ser Leu Glu Glu Ile Gln His Ala

2810 2815 2820

att gaa cac gca tct tta ggt aaa aag gaa tac tta agc caa gat ggt 8940

Ile Glu His Ala Ser Leu Gly Lys Lys Glu Tyr Leu Ser Gln Asp Gly

2825 2830 2835

gaa gaa gat aaa aag tta atg caa tta tgc tct ctg gaa atc acg cgc 8988

Glu Glu Asp Lys Lys Leu Met Gln Leu Cys Ser Leu Glu Ile Thr Arg

2840 2845 2850

agg agt tta aga tat cat tct cat ata gat aat gtg tcc tta aaa caa 9036

Arg Ser Leu Arg Tyr His Ser His Ile Asp Asn Val Ser Leu Lys Gln

2855 2860 2865 2870

gga act tta ctt tta gat gca tat aat ttt gtc tat cta tgc ata caa 9084

Gly Thr Leu Leu Leu Asp Ala Tyr Asn Phe Val Tyr Leu Cys Ile Gln

2875 2880 2885

cca tta tgt gat agc gtc aga ttg cat gaa aaa gcc gat ttt tta ttc 9132

Pro Leu Cys Asp Ser Val Arg Leu His Glu Lys Ala Asp Phe Leu Phe

2890 2895 2900

ctc agg gga aca ctg gac gat aat aat tac aat ttg tta atc gaa gat 9180

Leu Arg Gly Thr Leu Asp Asp Asn Asn Tyr Asn Leu Leu Ile Glu Asp

2905 2910 2915

gaa tat ggc ggt ttt tat aaa att aaa atg ccg gca aaa gct tct aat 9228

Glu Tyr Gly Gly Phe Tyr Lys Ile Lys Met Pro Ala Lys Ala Ser Asn

2920 2925 2930

att att tca ttt tca ttt gga gtc gaa aat gga aac ggt gtc atc ata 9276

Ile Ile Ser Phe Ser Phe Gly Val Glu Asn Gly Asn Gly Val Ile Ile

2935 2940 2945 2950

ggg aaa aag aac aat cta gtt aat act gac tat atc tca ttc gtt cct 9324

Gly Lys Lys Asn Asn Leu Val Asn Thr Asp Tyr Ile Ser Phe Val Pro

2955 2960 2965

tta ctc gtt gaa aaa ata tct act cca aaa gta ttg aaa tgg atc ggg 9372

Leu Leu Val Glu Lys Ile Ser Thr Pro Lys Val Leu Lys Trp Ile Gly

2970 2975 2980

gaa ata aaa aca acg tac gcg caa aaa ata aca act gat att gtt gct 9420

Glu Ile Lys Thr Thr Tyr Ala Gln Lys Ile Thr Thr Asp Ile Val Ala

2985 2990 2995

aat ctg tca aga ata ggt tta gat caa cat gag tgg tta cga ata aaa 9468

Asn Leu Ser Arg Ile Gly Leu Asp Gln His Glu Trp Leu Arg Ile Lys

3000 3005 3010

tca aaa gat ata taaatgatta tatatgccgt cgttttataa aaactggcgg 9520

Ser Lys Asp Ile

3015

catgtatatc tagttagtcc atcatagaag tcaagaaatt tagtttgccc tatatcttat 9580

agaaaatata ttttatatgc ttaaaaaaca ccatctttct aagatggcat ttatgtgctt 9640

tgtttcgatc aattacaact gatatattac catattgatt aattttatgt tatttaccaa 9700

agtaacggca tcttaatata tcgtcataat atagtgcgcg ttctgactct aatactgaaa 9760

aatttatttg ttctatttta cacttactgc aaatagcatc cagtttatca tatagtgtcg 9820

catcaattgg cgcag atg tca tca cgc caa atc ctt gag cat tat aat gct 9871

Met Ser Ser Arg Gln Ile Leu Glu His Tyr Asn Ala

3020 3025 3030

cta aca tat ccc cta cat caa tca atc ttg ttg cag ata atg act tcg 9919

Leu Thr Tyr Pro Leu His Gln Ser Ile Leu Leu Gln Ile Met Thr Ser

3035 3040 3045

aat ttg tta tca gtt tgc act gga aaa tcc att tac gag gat atc tcc 9967

Asn Leu Leu Ser Val Cys Thr Gly Lys Ser Ile Tyr Glu Asp Ile Ser

3050 3055 3060

ggc agt tct tgg aat atc ata cac ttc aat atc cct ctc ccc atc tct 10015

Gly Ser Ser Trp Asn Ile Ile His Phe Asn Ile Pro Leu Pro Ile Ser

3065 3070 3075

aga gcg aga ctt tcc ata ttt tct tat tgt gtc aga att aaa cct tgg 10063

Arg Ala Arg Leu Ser Ile Phe Ser Tyr Cys Val Arg Ile Lys Pro Trp

3080 3085 3090

atg agt atg gat tac atg taaccggctc atttaaaccg tctggtctgt 10111

Met Ser Met Asp Tyr Met

3095 3100

ttcctccggt tttacaaaaa ta atg tcc atc att ttt aat gga cac tat cgt 10163

Met Ser Ile Ile Phe Asn Gly His Tyr Arg

3105 3110

atg aaa cac cgg act tgg atc act gaa gct tta cgt ctt cac ttt gaa 10211

Met Lys His Arg Thr Trp Ile Thr Glu Ala Leu Arg Leu His Phe Glu

3115 3120 3125

gaa cat tta ccc cag gtt gtg gtc ggg cgt cgc ctg ggc gta cca aaa 10259

Glu His Leu Pro Gln Val Val Val Gly Arg Arg Leu Gly Val Pro Lys

3130 3135 3140

tca aca gct tgt ggt atg ttc gtg cgc ttt cgc aaa gct ggc ttt tca 10307

Ser Thr Ala Cys Gly Met Phe Val Arg Phe Arg Lys Ala Gly Phe Ser

3145 3150 3155

tgg cct ctg ccc gca ggt atg tcg gag cgg gag ctt gat ggc cgt ctt 10355

Trp Pro Leu Pro Ala Gly Met Ser Glu Arg Glu Leu Asp Gly Arg Leu

3160 3165 3170

tac ggg agt acc tcc aca gta cct gtc gta ctt tgt agt gga tcg gta 10403

Tyr Gly Ser Thr Ser Thr Val Pro Val Val Leu Cys Ser Gly Ser Val

3175 3180 3185 3190

att cag gac acc tcg aaa tcc tgt taatgttaaa acagtgaaaa tgaggtgatg 10457

Ile Gln Asp Thr Ser Lys Ser Cys

3195

c atg atc aaa act cgt cgg act aaa cgt acc ttt tcc ccg gag ttc aag 10506

Met Ile Lys Thr Arg Arg Thr Lys Arg Thr Phe Ser Pro Glu Phe Lys

3200 3205 3210

ctt gaa gct ttc gag cag gtg gtg gtt aaa tac cag cgt gat gtc aga 10554

Leu Glu Ala Phe Glu Gln Val Val Val Lys Tyr Gln Arg Asp Val Arg

3215 3220 3225 3230

gaa gtc gcg cag gca ctc gag ctc aac cct gac cat ttg cgt aaa tgg 10602

Glu Val Ala Gln Ala Leu Glu Leu Asn Pro Asp His Leu Arg Lys Trp

3235 3240 3245

ata cgg ttg tat aag cag gaa ctt cag ggt att gag cca gct ggt aat 10650

Ile Arg Leu Tyr Lys Gln Glu Leu Gln Gly Ile Glu Pro Ala Gly Asn

3250 3255 3260

gct att acc cct gaa caa cgc gaa att cag cag ctt aaa gcg cag ata 10698

Ala Ile Thr Pro Glu Gln Arg Glu Ile Gln Gln Leu Lys Ala Gln Ile

3265 3270 3275

aag cgc gtt gag atg gaa aaa gaa ata cta aag cag gct gcc gtg ctg 10746

Lys Arg Val Glu Met Glu Lys Glu Ile Leu Lys Gln Ala Ala Val Leu

3280 3285 3290

atg agc gaa atc ccc ggg aag ctg tcg cgc taatcacaca gctgaaagca 10796

Met Ser Glu Ile Pro Gly Lys Leu Ser Arg

3295 3300

aagtggccag tgtgggttat ttgtcattta ttcggtatta accgtagcgt ttattacgcg 10856

caggtgaagc gtcctgttaa tgtgcaaaga attgaattac gaagccgggt gagggctttc 10916

catgctctca gtcgtggcgc agccgggtag ccgggcaatc agtcagatgt tgcgccagag 10976

tggcgttgat gcaggccggt ggctggcatg acgactgatg cgggaatgag ggctgacaag 11036

tcgacagccg gttaaacatc acaaccgggt aaacgaagac aaaagtccgc cattgccaaa 11096

tttactgaac cggcaatttc accccgccgc accaaactgc gtctggtgcg gcgacatcag 11156

ttttattcg 11165

<210>35

<211>366

<212>PRT

<213>大肠杆菌

<400>35

Ser Asp Met Gln Arg Gly Ile Gln Ala Ala Thr Ala Ala Leu Gln Gly

1 5 10 15

Leu Val Gly Gly Asn Met Ala Gly Ala Leu Ala Gly Ala Ser Ala Pro

20 25 30

Glu Leu Ala Asn Ile Ile Gly His His Ala Gly Ile Asp Asp Asn Thr

35 40 45

Ala Ala Lys Ala Ile Ala His Ala Ile Leu Gly Gly Val Thr Ala Ala

50 55 60

Leu Gln Gly Asn Ser Ala Ala Ala Gly Ala Ile Gly Ala Gly Thr Gly

65 70 75 80

Glu Val Ile Ala Ser Ala Ile Ala Lys Ser Leu Tyr Pro Gly Val Asp

85 90 95

Pro Ser Lys Leu Thr Glu Asp Gln Lys Gln Thr Val Ser Thr Leu Ala

100 105 110

Thr Leu Ser Ala Gly Met Ala Gly Gly Ile Ala Ser Gly Asp Val Ala

115 120 125

Gly Ala Ala Ala Gly Ala Gly Ala Gly Lys Asn Val Val Glu Asn Asn

130 135 140

Ala Leu Ser Leu Val Ala Arg Gly Cys Ala Val Ala Ala Pro Cys Arg

145 150 155 160

Thr Lys Val Ala Glu Gln Leu Leu Glu Ile Gly Ala Lys Ala Gly Met

165 170 175

Ala Gly Leu Ala Gly Ala Ala Val Lys Asp Met Ala Asp Arg Met Thr

180 185 190

Ser Asp Glu Leu Glu His Leu Ile Thr Leu Gln Met Met Gly Asn Asp

195 200 205

Glu Ile Thr Thr Lys Tyr Leu Ser Ser Leu His Asp Lys Tyr Gly Ser

210 215 220

Gly Ala Ala Ser Asn Pro Asn Ile Gly Lys Asp Leu Thr Asp Ala Glu

225 230 235 240

Lys Val Glu Leu Gly Gly Ser Gly Ser Gly Thr Gly Thr Pro Pro Pro

245 250 255

Ser Glu Asn Asp Pro Lys Gln Gln Asn Glu Lys Thr Val Asp Lys Leu

260 265 270

Asn Gln Lys Gln Glu Ser Ala Ile Lys Lys Ile Asp Asn Thr Ile Lys

275 280 285

Asn Ala Leu Lys Asp His Asp Ile Ile Gly Thr Leu Lys Asp Met Asp

290 295 300

Gly Lys Pro Val Pro Lys Glu Asn Gly Gly Tyr Trp Asp His Met Gln

305 310 315 320

Glu Met Gln Asn Thr Leu Arg Gly Leu Arg Asn His Ala Asp Thr Leu

325 330 335

Lys Asn Val Asn Asn Pro Glu Ala Gln Ala Ala Tyr Gly Arg Ala Thr

340 345 350

Asp Ala Ile Asn Lys Ile Glu Ser Ala Leu Lys Gly Tyr Gly

355 360 365

<210>36

<211>128

<212>PRT

<213>大肠杆菌

<400>36

Met Ile Thr Leu Arg Lys Leu Ile Gly Asn Ile Asn Met Thr Lys Glu

1 5 10 15

Pro Glu Gln Gln Ser Pro Leu Glu Leu Trp Phe Glu Arg Ile Ile Asp

20 25 30

Val Pro Leu Glu Lys Leu Thr Val Glu Asp Leu Cys Arg Ala Ile Arg

35 40 45

Gln Asn Leu Cys Ile Asp Gln Leu Met Pro Arg Val Leu Glu Val Leu

50 55 60

Thr Lys Glu Pro Leu Ala Gly Glu Tyr Tyr Asp Gly Glu Leu Ile Ala

65 70 75 80

Ala Leu Ser Thr Ile Lys Gly Glu Asp Leu Lys Asp Gln Lys Ser Thr

85 90 95

Phe Thr Gln Ile Arg Gln Leu Ile Asn Gln Leu Glu Pro Ser Asp Ile

100 105 110

Asn Asp Asp Leu Arg Lys Asp Ile Leu Lys Ile Asn Gln Ile Ile Val

115 120 125

<210>37

<211>107

<212>PRT

<213>大肠杆菌

<400>37

Met Val Ala Lys Ala Phe Ala Tyr Ala Leu Asn Gln Trp Pro Ala Leu

1 5 10 15

Thr Tyr Tyr Ala Asn Asp Gly Trp Val Glu Ile Asp Asn Asn Ile Ala

20 25 30

Glu Asn Ala Leu Arg Ala Val Ser Leu Gly Arg Lys Asn Phe Leu Phe

35 40 45

Phe Gly Ser Asp His Gly Gly Glu Arg Gly Ala Leu Leu Tyr Ser Leu

50 55 60

Ile Gly Thr Cys Lys Leu Asn Asp Val Asp Pro Glu Ser Tyr Leu Arg

65 70 75 80

His Val Leu Ala Val Ile Ala Asp Trp Pro Val Asn Arg Val Ser Glu

85 90 95

Leu Leu Pro Trp Arg Ile Ala Leu Pro Ala Glu

100 105

<210>38

<211>86

<212>PRT

<213>大肠杆菌

<400>38

Met Leu Met Ser Val Gln Lys Glu Lys Asn Val Ala Glu Ser Val Val

1 5 10 15

Ser Glu Thr His Thr Gly Asp Ser Val Tyr A a Ser Leu Phe Glu Lys

20 25 30

Ile Asn Leu Asn Pro Val Ser Ala Leu Ser Ala Leu Asp Asn Pro Phe

35 40 45

Arg Ser Ala Asp Asn Ala Thr Gly Arg Ile Thr Ser Ser Ile Gln Pro

50 55 60

Ala Val Gln Cys Ala Ala Ala Ala Ala Thr Glu Gly Ser Cys Pro Arg

65 70 75 80

Gln Ser Pro Cys Ser Gly

85

<210>39

<211>111

<212>PRT

<213>大肠杆菌

<400>39

Met Val Asp Asn Trp Gln Lys Ser Val Arg Ser Arg Ala Leu Pro Glu

1 5 10 15

Glu Ala Met Thr Gly Trp Asn Glu Gly Met Ile Arg Leu Gln Gln Leu

20 25 30

Ala Glu Arg Leu Asn Arg Gln Asp Glu Gln Arg Gly Lys Tyr Met Thr

35 40 45

Val Ser Glu Leu Lys Thr Glu Val Phe Gly Ile Met Gln Ala Phe Asn

50 55 60

Arg His Ile Pro Ala Glu Glu Gln Leu Arg Arg Tyr Gly Glu Val Arg

65 70 75 80

Asn Gln Asn Gly Ser Glu Gln Gln Gln Lys Gln Ala Glu Met Ala Leu

85 90 95

Asn Gln Leu Ile Asn Arg Tyr Gln Met Ile Arg Ala Gly Lys Gln

100 105 110

<210>40

<211>143

<212>PRT

<213>大肠杆菌

<400>40

Met Val Gly Cys Ala Trp Leu Ala Glu Gln Ala Phe Ser Asp His Ala

1 5 10 15

Leu Ser Pro His Ser Ala Trp Pro Tyr Ser Ala Ser Arg Asp Ala Gly

20 25 30

Leu Ala Asp Thr Gly Ala Gly Gly Tyr Pro Thr Cys Lys Gln Arg Trp

35 40 45

Ala Asp Asp Thr Val Gly Leu Lys Ala Arg Leu Leu Gln Leu Pro Ala

50 55 60

Leu Asp Ile Trp Thr Ala Phe Lys Lys Ile Asp Gln Ser Gln Val Val

65 70 75 80

Tyr Glu Glu Ala Val Leu Arg Ser Arg Val Ser Glu Arg Asn Met Gln

85 90 95

Val Ser Gln Asn Gly Arg Val Tyr Pro Ser Tyr Gly Gly Asn Val Asp

100 105 110

Gly Thr Val Ala Asn Ala Ala Thr Arg Leu Ala Ser Gly Ala Arg Asn

115 120 125

Ile Leu Gly Ser Ile Ala Ala Cys Thr Ala Phe Asp Ser Val Arg

130 135 140

<210>41

<211>118

<212>PRT

<213>大肠杆菌

<400>41

Met Val Gln Ala Gln Leu Gln Ile Ala Leu Val Ile Cys Ile Pro Leu

1 5 10 15

Ile Thr Leu Cys Ser Ala Trp Asp Val Lys Val Val Met Thr Leu Thr

20 25 30

Phe Val Gln Phe Ala Leu Phe Phe Leu Thr Phe Trp Trp Glu Leu Ala

35 40 45

Arg Trp Leu Asp Ser Trp Leu Leu Asp Val Leu Tyr Asn Ser Asp Thr

50 55 60

His Ser Ser Trp Asn Leu Ala Gly Ile Gln Asn Thr Gln Asp Asp Val

65 70 75 80

Ile Ile Asn Leu Val Met Arg Leu Met Phe Leu Val Leu Pro Thr Phe

85 90 95

Trp Leu Gly Ala Met Thr Trp Ala Gly Val Arg Val Gly Val Ala Leu

100 105 110

Asn Gly Ala Leu Ala Gly

115

<210>42

<211>81

<212>PRT

<213>大肠杆菌

<400>42

Met Lys Tyr Leu Phe Phe Glu Asn Ile His Ser Ile Phe Leu Thr Phe

1 5 10 15

Ser Leu Phe Arg Thr Ser Val Ser Pro Asp Phe Pro Met Ile Phe Ala

20 25 30

Leu Pro Ser Ile Ile Leu Gly Gln Phe Thr Thr Asn Gln Leu Thr Asn

35 40 45

Phe Val Ile Cys Met Gly Asn Thr Val Glu Arg Arg Leu Gly Val Val

50 55 60

His Asn Pro Phe Lys Arg Ser Gly Asp Gly His Asp Leu Arg Ala Val

65 70 75 80

Ala

<210>43

<211>348

<212>PRT

<213>大肠杆菌

<400>43

Leu Ile Val Ile Asp Phe Phe Cys Gly Cys Gly Gly Ala Ser Glu Gly

1 5 10 15

Leu Arg Gln Ala Gly Phe Asp Ile Glu Leu Gly Leu Asp Ile Asp Gln

20 25 30

Gln Ala Ser Glu Thr Phe Lys Ala Asn Phe Pro Asp Ala Lys Phe Ile

35 40 45

Gln Asp Asp Ile Arg Lys Ile Glu Pro Gln Asp Ile Ser Asp Ile Ile

50 55 60

Asp Ile Lys Ala Lys Arg Pro Leu Leu Leu Ser Ala Cys Ala Pro Cys

65 70 75 80

Gln Pro Phe Ser Gln Gln Asn Lys Asn Lys Thr Ser Asp Asp Ser Arg

85 90 95

Arg Asn Leu Leu Asn Glu Thr His Arg Phe Ile Arg Glu Leu Leu Pro

100 105 110

Glu Tyr Ile Met Leu Glu Asn Val Pro Gly Met Gln Lys Ile Asp Glu

115 120 125

Glu Lys Glu Gly Pro Phe Gln Glu Phe Ile Lys Leu Leu Lys Glu Leu

130 135 140

Glu Tyr Asn Tyr Ile Ser Phe Ile Ala Asn Ala Glu Asn Tyr Gly Ile

145 150 155 160

Pro Gln Arg Arg Lys Arg Leu Val Leu Leu Ala Ser Arg Val Gly Lys

165 170 175

Val Thr Leu Pro Glu Ile Thr His Gly Lys Asn Lys Ile Pro Phe Lys

180 185 190

Thr Val Arg Asp Tyr Ile Gln Asp Phe Thr Lys Leu Cys Ser Gly Glu

195 200 205

Thr Asp Pro Lys Asp Pro Leu His Arg Ala Gly Thr Leu Ser Pro Leu

210 215 220

Asn Leu Lys Arg Ile Met His Thr Pro Glu Gly Gly Asp Arg Arg Asn

225 230 235 240

Trp Pro Glu Glu Leu Val Asn Lys Cys His Lys Asn Tyr Asp Gly His

245 250 255

Thr Asp Thr Tyr Gly Arg Met Ser Trp Asp Lys Pro Ala Pro Thr Leu

260 265 270

Thr Thr Lys Cys Asn Ser Tyr Ser Asn Gly Arg Phe Gly His Pro Asp

275 280 285

Pro Thr Gln His Arg Ala Ile Ser Ile Arg Glu Ala Ser Arg Leu Gln

290 295 300

Thr Phe Pro Leu Ser Tyr Val Phe Lys Gly Ser Leu Asn Ser Met Ala

305 310 315 320

Lys Gln Ile Gly Asn Ala Val Pro Cys Glu Leu Ala Arg Leu Phe Gly

325 330 335

Leu His Leu Ile Glu Asn Cys Thr Asn Lys Asp Ser

340 345

<210>44

<211>974

<212>PRT

<213>大肠杆菌

<400>44

Met Leu Gly Arg Gln Gln Ile Ala Gly Ile Pro Thr Ala Leu Ser Glu

1 5 10 15

Leu Phe Lys Asn Ala His Asp Ala Tyr Ala Asp Asn Val Glu Val Asp

20 25 30

Phe Phe Arg Lys Glu Asn Leu Leu Ile Leu Arg Asp Asp Gly Leu Gly

35 40 45

Met Thr Thr Asp Glu Phe Glu Glu Arg Trp Leu Thr Ile Gly Thr Ser

50 55 60

Ser Lys Leu Ile Asp Asp Asp Ala Ile Asn Lys Pro Ala Val Asp Ser

65 70 75 80

Asn Lys Ala Phe Arg Pro Ile Met Gly Glu Lys Gly Ile Gly Arg Leu

85 90 95

Ser Ile Ala Ala Ile Gly Pro Gln Val Leu Val Leu Thr Arg Ala Lys

100 105 110

Arg Asp Asn Glu Leu Lys Pro Leu Val Ala Ala Phe Val Asn Trp Ser

115 120 125

Leu Phe Ala Ile Pro Ser Leu Asp Leu Asp Asp Ile Glu Ile Pro Ile

130 135 140

Arg Thr Ile Ile Asn Asp Glu Cys Phe Thr Lys Lys Thr Leu Asp Glu

145 150 155 160

Met Ile Glu Gln Ala Arg Asn Asn Leu Asp Ser Leu Ser His Lys Ile

165 170 175

Ser Lys Ser Lys Val Ser Gln Ile Asn Thr Gln Leu Ser Ser Phe Glu

180 185 190

Phe Asp Pro Ile Leu Trp Glu Lys Lys Leu Gly Gly Leu Arg Leu Ser

195 200 205

Gly Asp Gly His Gly Thr His Phe Ile Ile Met Pro Thr Glu Glu Ile

210 215 220

Leu Ile Asp Asp Ile Ser Thr Ser Asp Ser Asn Lys Thr Ser Glu Gln

225 230 235 240

Ser Ser Arg Leu Glu Lys Ala Leu Leu Gly Phe Thr Asn Thr Met Tyr

245 250 255

Ser Asp Ser Asn Pro Pro Ile Ile Ala Arg Phe Arg Asp Tyr Leu Glu

260 265 270

Asp Gly Glu Cys Ile Asp Arg Ile Ser Glu Ser Ile Phe Phe Thr Pro

275 280 285

Gln Glu Phe Asn Leu Ala Asp His His Ile Glu Gly Trp Phe Asn Glu

290 295 300

Phe Gly Gln Phe Ser Gly Thr Val Ser Val Tyr Gly Glu Glu Pro Ile

305 310 315 320

His His Val Val Thr Trp Lys Asn Asn Asn Gln Leu Thr Gln Cys Gly

325 330 335

Pro Phe Lys Ile Lys Leu Ala Tyr Ile His Gly Arg Leu Arg Asp Ser

340 345 350

Arg Leu Pro Met Glu Leu Trp Ala Pro Leu Lys Glu Lys Thr Asp Arg

355 360 365

Tyr Gly Gly Leu Tyr Ile Tyr Arg Asp Gly Leu Arg Ile Leu Pro Tyr

370 375 380

Gly Asp Ser Asp Thr Asp Phe Leu Lys Ile Glu Lys Arg Arg Thr Leu

385 390 395 400

Ser Ala Ser Glu Tyr Phe Phe Ser Tyr Arg Arg Leu Phe Gly Ala Ile

405 410 415

Glu Leu Thr Lys Glu Asn Asn Ala Ser Leu Val Glu Lys Ala Gly Arg

420 425 430

Glu Gly Phe Ile Glu Asn Lys Pro Tyr Lys Gln Phe Lys Glu Met Leu

435 440 445

Glu Asn Phe Phe Ile Glu Ile Ala Arg Asp Phe Phe Lys Asp Asp Gly

450 455 460

Asp Met Ser Glu Leu Phe Val Glu Thr Lys Gln Arg Arg Asn Glu Glu

465 470 475 480

His Asp Leu Leu Ser Lys Arg Ser Lys Gln Thr Lys Ala Lys Lys Asp

485 490 495

Arg Leu Lys Lys Asp Leu Tyr Asp Phe Phe Asp Lys Leu Asp Asn Asp

500 505 510

Tyr Trp Asn Ile Glu Ile Asn Lys Leu Ile Asn Lys Asn Glu Glu Tyr

515 520 525

Phe Ser Ser Thr Glu Ile Thr Asp Thr Asn Ile Asp Tyr Val Tyr Asn

530 535 540

Lys Ile Lys Glu Gln Asn Asp Ala Ile Ile Lys Asn Leu Arg Asn Ser

545 550 555 560

Val Asp Ile Lys Lys Pro Ser Gly Val Gly Leu Thr Lys Glu Leu Ser

565 570 575

Asn Leu Trp Asp Arg Tyr Gln Ile Glu Arg Gln Lys Ile Leu Leu Ser

580 585 590

Leu Asn Glu Leu Lys Asp Asn Val Asp Arg Lys Leu Ile Glu Leu Asp

595 600 605

Asn Lys Asn Asn Asp Phe Leu Asn Leu Arg Lys Arg Leu Glu Asp Ser

610 615 620

Leu Asn Leu Gln Gln Ser Tyr Tyr Glu Lys Glu Leu Thr Lys Leu Tyr

625 630 635 640

Asn Asp Ala Lys Asn Ala Leu Lys Asp Val Gln Ser Lys Ala Asn Arg

645 650 655

Leu Ile Ser Asp Asn Lys Lys Lys His Lys Ser Glu Leu Lys Asn Ile

660 665 670

Ser Tyr Glu Phe Gln Ser Thr Asn Leu Asn Gly Lys Asp Thr Ala Tyr

675 680 685

Ile Leu Asp Val Lys Arg Asn Leu Glu Ser Lys Ile Glu Asn Thr Ser

690 695 700

Asn Glu Val Ile Asn Glu Ile Arg Lys Leu Thr Asp Gln Ile Ala Ile

705 710 715 720

Ile Ser Asp Ser Thr Thr Ser Glu Asn Leu Ser Ser Ala Gln Val Thr

725 730 735

Glu Ala Ile Glu Thr Glu Leu Glu His Leu Arg Asp Gln Gln Ala Asn

740 745 750

Asn Ala Glu Leu Ile Leu Leu Gly Met Ala Leu Ser Val Val His His

755 760 765

Glu Phe Asn Gly Asn Ile Arg Ala Ile Arg Ser Ala Leu Arg Glu Leu

770 775 780

Lys Ala Trp Ala Asp Arg Asn Pro Lys Leu Asp Ile Ile Tyr Gln Lys

785 790 795 800

Ile Arg Thr Ser Phe Asp His Leu Asp Gly Tyr Leu Lys Thr Phe Thr

805 810 815

Pro Leu Thr Arg Arg Leu Ser Arg Ser Lys Thr Asn Ile Thr Gly Thr

820 825 830

Ala Ile Leu Glu Phe Ile Arg Asp Val Phe Asp Asp Arg Leu Glu Lys

835 840 845

Glu Gly Ile Glu Leu Phe Thr Thr Ser Lys Phe Val Asn Gln Glu Ile

850 855 860

Val Thr Tyr Thr Ser Thr Ile Tyr Pro Val Phe Ile Asn Leu Ile Asp

865 870 875 880

Asn Ala Ile Tyr Trp Leu Gly Lys Thr Thr Gly Glu Lys Arg Leu Ile

885 890 895

Leu Asp Ala Thr Glu Thr Gly Phe Val Ile Gly Asp Thr Gly Pro Gly

900 905 910

Val Ser Thr Arg Asp Arg Asp Ile Ile Phe Asp Met Gly Phe Thr Arg

915 920 925

Lys Thr Gly Gly Arg Gly Met Gly Leu Phe Ile Ser Lys Glu Cys Leu

930 935 940

Ser Arg Asp Gly Phe Thr Ile Arg Leu Asp Asp Tyr Thr Pro Glu Gln

945 950 955 960

Gly Ala Phe Phe Ile Ile Glu Pro Ser Glu Glu Thr Ser Glu

965 970

<210>45

<211>555

<212>PRT

<213>大肠杆菌

<400>45

Met Thr Ser Ser Thr Asp Phe His Lys Leu Ser Glu Asp Cys Val Arg

1 5 10 15

Arg Phe Leu His Ser Val Val Ala Val Asp Asp Asn Met Ser Phe Gly

20 25 30

Ala Gly Ser Asp Thr Phe Pro Thr Asp Glu Asp Ile Asn Ala Leu Val

35 40 45

Asp Pro Asp Asp Asp Pro Thr Pro Ile Ile Thr Ala Ser Ala Ser Pro

50 55 60

Arg Ile Glu Ser Thr Lys Ser Lys Ala Lys Val Lys Asn His Pro Phe

65 70 75 80

Asp Tyr Gln Ala Leu Ala Glu Ala Phe Ala Lys Asp Gly Ile Ala Cys

85 90 95

Cys Gly Leu Leu Ala Lys Ser Phe Asn Val Glu Glu Arg Asp Ile Ile

100 105 110

Thr Ala Ser Ser His Lys Ala Asp Ile Thr Ile Leu Asp Trp Asp Met

115 120 125

Gln Ser Asp Ser Gly Gln Phe Ala Ile Glu Ile Ile Lys Ser Ile Ile

130 135 140

Val Ser Asp Ile Asn Ser Gly Gly Arg Leu Arg Leu Leu Ser Ile Tyr

145 150 155 160

Thr Gly Glu His Val Thr Ala Val Ile Thr Lys Leu Asn Asn Glu Leu

165 170 175

Lys Lys Thr Tyr Arg Ser Val Ile Lys Asn Asp Asp Ser Ile Phe Ile

180 185 190

Glu Asp Asn Tyr Ala Leu Glu Gln Trp Cys Ile Val Val Ile Ser Lys

195 200 205

Asp Val Tyr Glu Lys Asp Leu Pro Asn Val Leu Ile Lys Lys Phe Thr

210 215 220

Asn Leu Thr Ala Gly Leu Leu Ser Asn Ala Ala Leu Ser Cys Ile Ser

225 230 235 240

Glu Ile Arg Glu Lys Thr His Gly Ile Leu Thr Lys Tyr Asn Asn Lys

245 250 255

Leu Asp Thr Ala Tyr Val Ser His Ile Leu Asn Leu Ile Lys Ser Lys

260 265 270

Glu Ser Arg Ala Tyr Ala Tyr Glu Asn Ala His Asp Tyr Ala Val Asp

275 280 285

Leu Ile Ser Glu Glu Ile Arg Ser Ile Leu Gln Ile Ser Glu Asn Leu

290 295 300

Lys Lys Ser Leu Ser Lys Asn Ser Leu Ser His Trp Pro Ile Phe His

305 310 315 320

Tyr Ala Lys Asn Gly Cys Lys Asn Phe Leu Leu Thr Gly Lys Lys Gln

325 330 335

Lys Asp Leu Ser Val Glu His Leu Arg Asn Ile Leu Ser Ala Asp Ser

340 345 350

Leu Glu Glu Ile Gln His Ala Ile Glu His Ala Ser Leu Gly Lys Lys

355 360 365

Glu Tyr Leu Ser Gln Asp Gly Glu Glu Asp Lys Lys Leu Met Gln Leu

370 375 380

Cys Ser Leu Glu Ile Thr Arg Arg Ser Leu Arg Tyr His Ser His Ile

385 390 395 400

Asp Asn Val Ser Leu Lys Gln Gly Thr Leu Leu Leu Asp Ala Tyr Asn

405 410 415

Phe Val Tyr Leu Cys Ile Gln Pro Leu Cys Asp Ser Val Arg Leu His

420 425 430

Glu Lys Ala Asp Phe Leu Phe Leu Arg Gly Thr Leu Asp Asp Asn Asn

435 440 445

Tyr Asn Leu Leu Ile Glu Asp Glu Tyr Gly Gly Phe Tyr Lys Ile Lys

450 455 460

Met Pro Ala Lys Ala Ser Asn Ile Ile Ser Phe Ser Phe Gly Val Glu

465 470 475 480

Asn Gly Asn Gly Val Ile Ile Gly Lys Lys Asn Asn Leu Val Asn Thr

485 490 495

Asp Tyr Ile Ser Phe Val Pro Leu Leu Val Glu Lys Ile Ser Thr Pro

500 505 510

Lys Val Leu Lys Trp Ile Gly Glu Ile Lys Thr Thr Tyr Ala Gln Lys

515 520 525

Ile Thr Thr Asp Ile Val Ala Asn Leu Ser Arg Ile Gly Leu Asp Gln

530 535 540

His Glu Trp Leu Arg Ile Lys Ser Lys Asp Ile

545 550 555

<210>46

<211>82

<212>PRT

<213>大肠杆菌

<400>46

Met Ser Ser Arg Gln Ile Leu Glu His Tyr Asn Ala Leu Thr Tyr Pro

1 5 10 15

Leu His Gln Ser Ile Leu Leu Gln Ile Met Thr Ser Asn Leu Leu Ser

20 25 30

Val Cys Thr Gly Lys Ser Ile Tyr Glu Asp Ile Ser Gly Ser Ser Trp

35 40 45

Asn Ile Ile His Phe Asn Ile Pro Leu Pro Ile Ser Arg Ala Arg Leu

50 55 60

Ser Ile Phe Ser Tyr Cys Val Arg Ile Lys Pro Trp Met Ser Met Asp

65 70 75 80

Tyr Met

<210>47

<211>98

<212>PRT

<213>大肠杆菌

<400>47

Met Ser Ile Ile Phe Asn Gly His Tyr Arg Met Lys His Arg Thr Trp

1 5 10 15

Ile Thr Glu Ala Leu Arg Leu His Phe Glu Glu His Leu Pro Gln Val

20 25 30

Val Val Gly Arg Arg Leu Gly Val Pro Lys Ser Thr Ala Cys Gly Met

35 40 45

Phe Val Arg Phe Arg Lys Ala Gly Phe Ser Trp Pro Leu Pro Ala Gly

50 55 60

Met Ser Glu Arg Glu Leu Asp Gly Arg Leu Tyr Gly Ser Thr Ser Thr

65 70 75 80

Val Pro Val Val Leu Cys Ser Gly Ser Val Ile Gln Asp Thr Ser Lys

85 90 95

Ser Cys

<210>48

<211>106

<212>PRT

<213>大肠杆菌

<400>48

Met Ile Lys Thr Arg Arg Thr Lys Arg Thr Phe Ser Pro Glu Phe Lys

1 5 10 15

Leu Glu Ala Phe Glu Gln Val Val Val Lys Tyr Gln Arg Asp Val Arg

20 25 30

Glu Val Ala Gln Ala Leu Glu Leu Asn Pro Asp His Leu Arg Lys Trp

35 40 45

Ile Arg Leu Tyr Lys Gln Glu Leu Gln Gly Ile Glu Pro Ala Gly Asn

50 55 60

Ala Ile Thr Pro Glu Gln Arg Glu Ile Gln Gln Leu Lys Ala Gln Ile

65 70 75 80

Lys Arg Val Glu Met Glu Lys Glu Ile Leu Lys Gln Ala Ala Val Leu

85 90 95

Met Ser Glu Ile Pro Gly Lys Leu Ser Arg

100 105

<210>49

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>49

tgctctagag ccattactca gaatggg 27

<210>50

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>50

cgcgagctcg acgactgaat gatccc 26

<210>51

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>51

tcccccgggt actgcagcac tcaacc 26

<210>52

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>52

gatcccggga ccactgaaat gcgtgc 26

<210>53

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>53

tcgtctagag atgatggtga tggagcg 27

<210>54

<211>28

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>54

gaactgcagc caaatactga taccaccc 28

<210>55

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>55

gaactgcagg ctaaaacaga agacgcg 27

<210>56

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>56

catgcatgca ctccatatga caaccgc 27

<210>57

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>57

tcgtctagaa tgaagctgcg catgagg 27

<210>58

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>58

caactgcagt cgcaaattgc gaactgg 27

<210>59

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>59

caactgcaga ccgcaacttt tcgacgc 27

<210>60

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>60

catgcatgcc agtgagccat tgttccc 27

<210>61

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>61

tgctctagat acgactctga caggagg 27

<210>62

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>62

tcagatatca actaccagca gtttgg 26

<210>63

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>63

tcagatatcc ataaagagtg acgtggc 27

<210>64

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>64

tgctctagaa aacgtggcaa cagagcg 27

<210>65

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>65

tgctctagaa ggcgttgtcg atcctg 26

<210>66

<211>28

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>66

gaactgcagg aaaaggccga gcagactg 28

<210>67

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>67

gaactgcagt acagccatgt ttacggt 27

<210>68

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>68

catgcatgcg gtgtacgaca gtttgcg 27

<210>69

<211>26

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>69

tgctctagac acatcatggg cacacc 26

<210>70

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>70

gaactgcaga accgtccaca tcaggcg 27

<210>71

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>71

gaactgcaga ccctgcttgc cattccg 27

<210>72

<211>27

<212>DNA

<213>人工序列

<220>

<223>人工序列描述：寡核苷酸

<400>72

catgcatgca taagcgtcga acaggcg 27

Claims

1.一种用于治疗用途的获自大肠杆菌K1的本文鉴定为mdoG的肽、或其在革兰氏阴性菌中在氨基酸水平上至少具有60％同源性的同源物或其功能片段。

2.一种根据权利要求1所述的肽，它包括本文鉴定为SEQ ID NO.2的氨基酸序列。

3.一种用于治疗用途的编码权利要求1或权利要求2的肽的多核苷酸。

4.一种用于表达权利要求1或权利要求2的肽的转化的宿主。

5.一种包括权利要求1或权利要求2的肽的疫苗，或用于其表达的工具。

6.一种包括带有毒力基因突变的微生物的疫苗，其中所述的基因编码权利要求1或权利要求2的肽。

7.权利要求1-4中任意一项所述的产物在筛选有潜能的药物或在检测毒性中的用途。

8.权利要求1-4中任意一项所述的产物在制备用于治疗或预防与革兰氏阴性菌导致的感染有关的疾病的药物中的用途。

9.根据权利要求8所述的用途，其中所述的细菌是大肠杆菌。