CN1250561C - 一种葛根总黄酮的提取方法 - Google Patents
一种葛根总黄酮的提取方法 Download PDFInfo
- Publication number
- CN1250561C CN1250561C CN 03115538 CN03115538A CN1250561C CN 1250561 C CN1250561 C CN 1250561C CN 03115538 CN03115538 CN 03115538 CN 03115538 A CN03115538 A CN 03115538A CN 1250561 C CN1250561 C CN 1250561C
- Authority
- CN
- China
- Prior art keywords
- extraction
- radix puerariae
- flavonoids
- chitosan
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 235000011949 flavones Nutrition 0.000 title claims abstract description 31
- 229930003944 flavone Natural products 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 24
- 235000010575 Pueraria lobata Nutrition 0.000 title claims abstract description 19
- 244000046146 Pueraria lobata Species 0.000 title claims abstract description 18
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title abstract description 7
- 150000002212 flavone derivatives Chemical class 0.000 title abstract description 7
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title abstract description 7
- 229920001661 Chitosan Polymers 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims abstract description 9
- 150000002213 flavones Chemical class 0.000 claims description 22
- 238000000605 extraction Methods 0.000 claims description 15
- 239000011347 resin Substances 0.000 claims description 14
- 229920005989 resin Polymers 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N Daidzein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 claims description 12
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 claims description 11
- 229930003935 flavonoid Natural products 0.000 claims description 10
- 150000002215 flavonoids Chemical class 0.000 claims description 10
- 235000017173 flavonoids Nutrition 0.000 claims description 10
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 240000006859 Jasminum officinale Species 0.000 claims description 5
- 235000010254 Jasminum officinale Nutrition 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- GMTUGPYJRUMVTC-UHFFFAOYSA-N Daidzin Natural products OC(COc1ccc2C(=O)C(=COc2c1)c3ccc(O)cc3)C(O)C(O)C(O)C=O GMTUGPYJRUMVTC-UHFFFAOYSA-N 0.000 claims description 4
- KYQZWONCHDNPDP-UHFFFAOYSA-N Daidzoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-UHFFFAOYSA-N 0.000 claims description 4
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 4
- 235000007240 daidzein Nutrition 0.000 claims description 4
- KYQZWONCHDNPDP-QNDFHXLGSA-N daidzein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-QNDFHXLGSA-N 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 230000006196 deacetylation Effects 0.000 claims description 2
- 238000003381 deacetylation reaction Methods 0.000 claims description 2
- -1 filter Substances 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 24
- 238000003809 water extraction Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000010828 elution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 235000008504 concentrate Nutrition 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 241001126925 Lobata Species 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 241000219780 Pueraria Species 0.000 description 1
- 241000219781 Pueraria montana var. lobata Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 229940046892 lead acetate Drugs 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000003808 methanol extraction Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03115538 CN1250561C (zh) | 2003-02-27 | 2003-02-27 | 一种葛根总黄酮的提取方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03115538 CN1250561C (zh) | 2003-02-27 | 2003-02-27 | 一种葛根总黄酮的提取方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1524867A CN1524867A (zh) | 2004-09-01 |
CN1250561C true CN1250561C (zh) | 2006-04-12 |
Family
ID=34284331
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 03115538 Expired - Lifetime CN1250561C (zh) | 2003-02-27 | 2003-02-27 | 一种葛根总黄酮的提取方法 |
Country Status (1)
Country | Link |
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CN (1) | CN1250561C (zh) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7482029B2 (en) | 2005-04-01 | 2009-01-27 | Bionovo, Inc. | Composition for treatment of menopause |
CN101190908B (zh) * | 2006-11-22 | 2010-08-25 | 中国科学院过程工程研究所 | 葛根发酵燃料乙醇剩余物总黄酮提取的方法 |
CN101007045B (zh) * | 2007-01-23 | 2010-05-19 | 华南理工大学 | 利用磁性吸附树脂及外加磁场分离纯化葛根黄酮的方法 |
CN103224491A (zh) * | 2013-05-22 | 2013-07-31 | 天津道谷生物科技有限公司 | 以水为溶媒提取高纯葛根素的方法 |
CN103223008A (zh) * | 2013-05-22 | 2013-07-31 | 天津道谷生物科技有限公司 | 以水为溶媒提取高纯葛根黄酮的方法 |
-
2003
- 2003-02-27 CN CN 03115538 patent/CN1250561C/zh not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
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CN1524867A (zh) | 2004-09-01 |
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C06 | Publication | ||
PB01 | Publication | ||
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SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shanghai Guanshengyuan Huatuo Winery Co.,Ltd. Assignor: Shanghai University of Traditional Chinese Medicine Contract fulfillment period: 2006.12.1 to 2011.11.30 Contract record no.: 2008310000020 Denomination of invention: Method for extracting kudzuvine root total flavone Granted publication date: 20060412 License type: Exclusive license Record date: 20080909 |
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LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENCE; TIME LIMIT OF IMPLEMENTING CONTACT: 2006.12.1 TO 2011.11.30 Name of requester: SHANGHAI GUANSHENGYUAN HUATUO WINE CO., LTD. Effective date: 20080909 |
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ASS | Succession or assignment of patent right |
Owner name: SHANGHAI INOHERB COSMETICS CO., LTD. Free format text: FORMER OWNER: SHANGHAI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE Effective date: 20110817 |
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C41 | Transfer of patent application or patent right or utility model | ||
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Free format text: CORRECT: ADDRESS; FROM: 200032 XUHUI, SHANGHAI TO: 200283 HONGKOU, SHANGHAI |
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TR01 | Transfer of patent right |
Effective date of registration: 20110817 Address after: 200283 Shanghai City, Hongkou District Huayuan Road 66, Lane 1, building 18-19, Jiahe International Building Patentee after: SHANGHAI INOHERB COSMETIC Co.,Ltd. Address before: 200032 No. 530, Lingling Road, Shanghai Patentee before: Shanghai University of Traditional Chinese Medicine |
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C56 | Change in the name or address of the patentee | ||
CP02 | Change in the address of a patent holder |
Address after: 200444 Shanghai city Baoshan District City Bank Road No. 121 Patentee after: SHANGHAI INOHERB COSMETIC Co.,Ltd. Address before: 200283 Shanghai City, Hongkou District Huayuan Road 66, Lane 1, building 18-19, Jiahe International Building Patentee before: SHANGHAI INOHERB COSMETIC Co.,Ltd. |
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DD01 | Delivery of document by public notice |
Addressee: Huang Jing Document name: Notification of Passing Examination on Formalities |
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CX01 | Expiry of patent term |
Granted publication date: 20060412 |
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