From the plant Capparis fruit, extract the method for arthritis effective site
Technical field
The present invention relates to a kind of method of from the plant Capparis fruit, extracting arthritis effective site.
Background technology
Capparis fruit claims (Cortex Capparis Spinosae Radicis) for the fruit of Capparidaceae Chinese lime platymiscium Cortex Capparis Spinosae Radicis Capparisspinosa L again, has another name called Herba hibisci trioni.The desert Gobi desert is born in happiness and low hillside stone matter sandy soil are located in.There is distribution on ground such as Xinjiang of China, Tibet.That this product is is avette, Long Circle or class sphere, and long 2-4 centimetre, wide 1.5-3 centimetre, surperficial yellow green is to pale brown color; The blunt circle in top or truncate.Vertical rib 5-7 bar is all arranged, how not remarkable, only obvious in the lower end.Seed bonds agglomerating mutually, can see the granular profile of projection from the outside, and peel and seed tight bond are together.Among the people long-term with diseases such as Capparis fruit (Cortex Capparis Spinosae Radicis) treatment of arthritis, gouts, effect is remarkable.
As everyone knows, rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis etc. are common clinical, frequently-occurring disease, and it is a lot of to treat this type of sick Chinese and western drugs.The present invention is according to Uygur's theory of medicine, extracts arthritis effective site from the plant Capparis fruit, provides new approach for being used for the treatment of diseases such as rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis.The present invention does not see the extraction and separation technology and the relevant patent of relevant plant Capparis fruit treatment rheumatic arthritis aspect effective site through to patent retrieval in nearly 15 years both at home and abroad.
Summary of the invention
The objective of the invention is to, a kind of method of extracting arthritis effective site from the plant Capparis fruit is provided, this method is with plant Capparis fruit raw material pulverizing, and reflux, merceration or percolation extract, and concentrating under reduced pressure obtains crude extract; Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract, obtain ethyl acetate effective site and n-butyl alcohol effective site respectively; Through evidence: this effective site has the effect of analgesia, antiinflammatory and inhibition immunity, is used for the treatment of diseases such as rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis.This method is safe and reliable, effectively, high yield, low cost is for diseases such as treatment rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis provide new approach.
The method of extracting arthritis effective site from the plant Capparis fruit of the present invention follows these steps to carry out:
A, Capparis fruit is pulverized the 10-40 order, carry out reflux with 50%-90% methanol or ethanol, merceration or percolation extract, raw material and solvent ratios are 1: 5-20, extract 1-3 time, extraction time is 2-10 hour, and extracting solution can obtain Capparis fruit arthritis crude extract at 30-60 ℃ of concentrating under reduced pressure;
B, earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract, obtain containing the n-butyl alcohol effective site and the ethyl acetate effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively.
From the plant Capparis fruit, extract the n-butyl alcohol effective site and the application of ethyl acetate effective site in preparation anti-inflammatory and antalgic, antiinflammatory, immunoregulation medicament that obtain in the method for arthritis effective site.
The ethyl acetate effective site that the present invention relates to has remarkable pharmacological action:
1, anti-inflammatory and analgesic effect
Table 1 Dichlorodiphenyl Acetate cause the mouse writhing number of times influence (n=10, X ± SD):
Group | Dosage (mg/kg) | Turn round body number of times (n=10) |
Blank group dolantin matched group ethyl acetate effective site n-butyl alcohol effective site | Normal saline 15 15 15 | 21.5±12.1 9.9±11.7
** 12.1±11.6
* 15.2±11.7
|
2, antiinflammatory test:
The influence of table 2 pair mouse peritoneal capillary permeability (n=10, X ± SD)
Group | Dosage (mg/kg) | A value (590nm) | Suppression ratio (%) |
Blank group 0.04% prednisone matched group ethyl acetate effective site n-butyl alcohol effective site | 8 15 15 | 0.46±0.19 0.30±0.16
* 0.22±0.09
** 0.24±0.11
** | 36.96 52.61 47.83 |
3, immunomodulating test:
The influence that hemolysin forms in the table 3 pair normal mice serum (n=10, X ± SD)
Group | Dosage (mg/kg) | Hemolysin O D value |
Blank group 0.04% prednisone matched group ethyl acetate effective site n-butyl alcohol effective site | 8 15 15 | 0.0220±0.1000 0.0095±0.0021
** 0.0089±0.0040
* 0.0160±0.0100
* |
The specific embodiment
Embodiment 1:
4 kilograms of Capparis fruits are pulverized 10 orders, carry out reflux with 80 liter of 50% methanol, merceration extracts, and extracts 3 times, and extraction time is 2 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 40 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 2:
5 kilograms of Capparis fruits are pulverized 20 orders, carry out reflux with 50 liter of 70% ethanol, percolation extracts, and extracts 2 times, and extraction time is 5 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 45 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 3
4.5 kilograms of Capparis fruits are pulverized 30 orders, carry out reflux with 60 liter of 80% methanol, merceration extracts, and extracts 1 time, and extraction time is 10 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 50 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 4
5.5 kilograms of Capparis fruits are pulverized 40 orders, carry out reflux with 100 liter of 90% ethanol, percolation extracts, and extracts 1 time, and extraction time is 10 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 60 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).