CN1234375C - Method for extracting active arthritis resisting element from Capparis fruit - Google Patents
Method for extracting active arthritis resisting element from Capparis fruit Download PDFInfo
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- CN1234375C CN1234375C CN 200310113957 CN200310113957A CN1234375C CN 1234375 C CN1234375 C CN 1234375C CN 200310113957 CN200310113957 CN 200310113957 CN 200310113957 A CN200310113957 A CN 200310113957A CN 1234375 C CN1234375 C CN 1234375C
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Abstract
The present invention relates to a method for extracting effective parts from plant mallet fruits and vine seeds for resisting arthritis. The method comprises the following steps: crushing raw materials of plant mallet fruits and vine seeds, heating and refluxing the raw materials, extracting the raw materials, and reducing pressure for concentrating the raw materials to obtain crude extracts; degreasing the crude extracts by petroleum ether, and eluting residues by an ethyl acetate system and a normal butyl alcohol system to respectively obtain different effective parts. Experiments prove that the effective parts have the functions of alleviating pain, resisting inflammation and restraining the immunity and are used for treating diseases, such as rheumatoid arthritis, proliferative arthritis, gout, periarthritis of shoulder, etc. The method has the advantages of high safety, reliability, efficiency, high yield and low cost. The present invention provides a new path for treating diseases, such as rheumatoid arthritis, proliferative arthritis, gout, periarthritis of shoulder, etc.
Description
Technical field
The present invention relates to a kind of method of from the plant Capparis fruit, extracting arthritis effective site.
Background technology
Capparis fruit claims (Cortex Capparis Spinosae Radicis) for the fruit of Capparidaceae Chinese lime platymiscium Cortex Capparis Spinosae Radicis Capparisspinosa L again, has another name called Herba hibisci trioni.The desert Gobi desert is born in happiness and low hillside stone matter sandy soil are located in.There is distribution on ground such as Xinjiang of China, Tibet.That this product is is avette, Long Circle or class sphere, and long 2-4 centimetre, wide 1.5-3 centimetre, surperficial yellow green is to pale brown color; The blunt circle in top or truncate.Vertical rib 5-7 bar is all arranged, how not remarkable, only obvious in the lower end.Seed bonds agglomerating mutually, can see the granular profile of projection from the outside, and peel and seed tight bond are together.Among the people long-term with diseases such as Capparis fruit (Cortex Capparis Spinosae Radicis) treatment of arthritis, gouts, effect is remarkable.
As everyone knows, rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis etc. are common clinical, frequently-occurring disease, and it is a lot of to treat this type of sick Chinese and western drugs.The present invention is according to Uygur's theory of medicine, extracts arthritis effective site from the plant Capparis fruit, provides new approach for being used for the treatment of diseases such as rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis.The present invention does not see the extraction and separation technology and the relevant patent of relevant plant Capparis fruit treatment rheumatic arthritis aspect effective site through to patent retrieval in nearly 15 years both at home and abroad.
Summary of the invention
The objective of the invention is to, a kind of method of extracting arthritis effective site from the plant Capparis fruit is provided, this method is with plant Capparis fruit raw material pulverizing, and reflux, merceration or percolation extract, and concentrating under reduced pressure obtains crude extract; Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract, obtain ethyl acetate effective site and n-butyl alcohol effective site respectively; Through evidence: this effective site has the effect of analgesia, antiinflammatory and inhibition immunity, is used for the treatment of diseases such as rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis.This method is safe and reliable, effectively, high yield, low cost is for diseases such as treatment rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis provide new approach.
The method of extracting arthritis effective site from the plant Capparis fruit of the present invention follows these steps to carry out:
A, Capparis fruit is pulverized the 10-40 order, carry out reflux with 50%-90% methanol or ethanol, merceration or percolation extract, raw material and solvent ratios are 1: 5-20, extract 1-3 time, extraction time is 2-10 hour, and extracting solution can obtain Capparis fruit arthritis crude extract at 30-60 ℃ of concentrating under reduced pressure;
B, earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract, obtain containing the n-butyl alcohol effective site and the ethyl acetate effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively.
From the plant Capparis fruit, extract the n-butyl alcohol effective site and the application of ethyl acetate effective site in preparation anti-inflammatory and antalgic, antiinflammatory, immunoregulation medicament that obtain in the method for arthritis effective site.
The ethyl acetate effective site that the present invention relates to has remarkable pharmacological action:
1, anti-inflammatory and analgesic effect
Table 1 Dichlorodiphenyl Acetate cause the mouse writhing number of times influence (n=10, X ± SD):
| Group | Dosage (mg/kg) | Turn round body number of times (n=10) |
| Blank group dolantin matched group ethyl acetate effective site n-butyl alcohol effective site | Normal saline 15 15 15 | 21.5±12.1 9.9±11.7 ** 12.1±11.6 * 15.2±11.7 |
2, antiinflammatory test:
The influence of table 2 pair mouse peritoneal capillary permeability (n=10, X ± SD)
| Group | Dosage (mg/kg) | A value (590nm) | Suppression ratio (%) |
| Blank group 0.04% prednisone matched group ethyl acetate effective site n-butyl alcohol effective site | 8 15 15 | 0.46±0.19 0.30±0.16 * 0.22±0.09 ** 0.24±0.11 ** | 36.96 52.61 47.83 |
3, immunomodulating test:
The influence that hemolysin forms in the table 3 pair normal mice serum (n=10, X ± SD)
| Group | Dosage (mg/kg) | Hemolysin O D value |
| Blank group 0.04% prednisone matched group ethyl acetate effective site n-butyl alcohol effective site | 8 15 15 | 0.0220±0.1000 0.0095±0.0021 ** 0.0089±0.0040 * 0.0160±0.0100 * |
The specific embodiment
Embodiment 1:
4 kilograms of Capparis fruits are pulverized 10 orders, carry out reflux with 80 liter of 50% methanol, merceration extracts, and extracts 3 times, and extraction time is 2 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 40 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 2:
5 kilograms of Capparis fruits are pulverized 20 orders, carry out reflux with 50 liter of 70% ethanol, percolation extracts, and extracts 2 times, and extraction time is 5 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 45 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 3
4.5 kilograms of Capparis fruits are pulverized 30 orders, carry out reflux with 60 liter of 80% methanol, merceration extracts, and extracts 1 time, and extraction time is 10 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 50 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Embodiment 4
5.5 kilograms of Capparis fruits are pulverized 40 orders, carry out reflux with 100 liter of 90% ethanol, percolation extracts, and extracts 1 time, and extraction time is 10 hours, and merge extractive liquid,, extracting solution can obtain Capparis fruit arthritis crude extract extractum at 60 ℃ of concentrating under reduced pressure;
Earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract extractum, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively;
Again the ethyl acetate effective site and the n-butyl alcohol effective site that obtain are mixed as the medicinal application for preparing diseases such as treating rheumatic arthritis, rheumatoid arthritis, gout, scapulohumeral periarthritis with arbitrary proportion separately or with other medicines (comprising Western medicine or Chinese medicine).
Claims (2)
1, a kind of method of extracting arthritis effective site from the plant Capparis fruit is characterized in that following these steps to carrying out:
A, Capparis fruit is pulverized the 10-40 order, carry out reflux with 50%-90% methanol or ethanol, merceration or percolation extract, raw material and solvent ratios are 1: 5-20, extract 1-3 time, extraction time is 2-10 hour, and extracting solution can obtain Capparis fruit arthritis crude extract at 30-60 ℃ of concentrating under reduced pressure;
B, earlier use defat with petroleum ether, residue to use ethyl acetate and n-butyl alcohol system eluting successively crude extract, obtain containing the ethyl acetate effective site and the n-butyl alcohol effective site of one or more Palmic acid or caffeic acid and coffee aldehyde or protocatechualdehyde respectively.
2, according to claim 1ly from the plant Capparis fruit, extract ethyl acetate effective site and the application of n-butyl alcohol effective site in preparation anti-inflammatory and antalgic, antiinflammatory, immunoregulation medicament that obtains in the method for arthritis effective site.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310113957 CN1234375C (en) | 2003-11-05 | 2003-11-05 | Method for extracting active arthritis resisting element from Capparis fruit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310113957 CN1234375C (en) | 2003-11-05 | 2003-11-05 | Method for extracting active arthritis resisting element from Capparis fruit |
Publications (2)
| Publication Number | Publication Date |
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| CN1541700A CN1541700A (en) | 2004-11-03 |
| CN1234375C true CN1234375C (en) | 2006-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200310113957 Expired - Fee Related CN1234375C (en) | 2003-11-05 | 2003-11-05 | Method for extracting active arthritis resisting element from Capparis fruit |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100574781C (en) * | 2006-03-28 | 2009-12-30 | 通威股份有限公司 | A kind of veterinary drug for the treatment of gout and its production and use |
| CN101185663B (en) * | 2006-11-15 | 2010-08-11 | 上海医药工业研究院 | Method for obtaining the extraction from caper medicinal part and application of the extraction |
| CN101244099B (en) * | 2008-02-22 | 2011-03-16 | 浙江大学 | Method for preparing valid target in gavel fruit vine fruit and application of the same |
| CN101474217B (en) * | 2009-01-21 | 2013-01-16 | 石河子大学医学院第一附属医院 | Antiphlogistic analgesic Capparis spinosa L. catablasm and preparation method thereof |
| CN102423323B (en) * | 2011-12-12 | 2013-03-13 | 中国科学院新疆理化技术研究所 | Compound medicine for treating rheumatism and preparation method thereof |
| CN103623029A (en) * | 2013-11-06 | 2014-03-12 | 广西中医药大学 | Watermelon vine extract with inflammation-diminishing and pain-relieving effects, as well as preparation method and application thereof |
| CN109381497A (en) * | 2017-08-08 | 2019-02-26 | 上海医药工业研究院 | Capparis spinosa extract, preparation method and application |
| CN115844932B (en) * | 2023-02-03 | 2023-11-24 | 新疆前进荣耀投资有限公司 | Caper extract D95 and preparation method and application thereof |
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Owner name: XINJIANG UIGUR PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: THE XINJIANG TECHNICAL INSTITUTE OF PHYSICS + CHEMISTRY, CHINESE ACADEMY OF SCIE Effective date: 20110315 |
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