CN102657698A - Extraction method and application of salvia plebeia general flavone extract - Google Patents
Extraction method and application of salvia plebeia general flavone extract Download PDFInfo
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- CN102657698A CN102657698A CN2012101742654A CN201210174265A CN102657698A CN 102657698 A CN102657698 A CN 102657698A CN 2012101742654 A CN2012101742654 A CN 2012101742654A CN 201210174265 A CN201210174265 A CN 201210174265A CN 102657698 A CN102657698 A CN 102657698A
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- salvia plebeia
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Abstract
The invention relates to an extraction method of salvia plebeia general flavone extract. The extraction method comprises the following steps: after smashing an aboveground part of salvia plebeia, decocting with water, concentrating to obtain a salvia plebeia concentrate, enabling the salvia plebeia concentrate to pass through pretreated polyamide, washing a polyamide column, then using an eluent for elution, collecting a solution after elution, and concentrating to be dry, thus obtaining the salvia plebeia general flavone extract. Compared with the existing extraction method, the method for preparing general flavone in salvia plebeia provided by the invention has the following obvious advantages: firstly, polyamide resin has the characteristic of strong specificity, and can be used for effectively adsorbing and removing compositions of phenolic acids and saponins in extract, while macroporous resin does not have such capability; secondly, extraction solvent water is used for replacing ethanol so as to not only be simple in operation, but also effectively save energy; and thirdly, the content of the extracted general flavone is as high as above 80%. The salvia plebeia general flavone extract which is extracted by the extraction method has significant anti-inflammatory and bacteriostatic effects, and certain therapeutical effect on acute pharyngitis model rats.
Description
One, technical field:
The present invention relates to the extractive technique field of Chinese herbal medicine, more particularly relate to Herba Salviae Plebeiae total flavones extractive technique field, be meant the method for distilling and the purposes of common sage herb general flavone extract especially, it has the effect of obvious treatment pharyngitis.
Two, background technology:
Chronic pharyngitis is a kind of common disease, and frequently-occurring disease mainly shows as pharynx mucosa chronic inflammatory disease, and symptoms such as diffuse inflammation often are the part of upper respiratory tract chronic inflammatory disease, the then many inflammation with the pharynx lymphoid tissue of limitation inflammation.The cause of disease of chronic pharyngitis can not generally be discussed; But with following factors very directly relation is arranged: excessively can make throat portion swelling inflammation with throat; In the course of time will become chronic pharyngitis and intractable pharyngitis, crowds' such as teacher, operator and traffic police occupation disease is exactly that this reason causes; Environmental stimuli also is one of major reason that causes chronic pharyngitis, like long smoking and drinking, too much eats irritable foods such as Fructus Capsici, long-term work study etc. under deleterious working environment; The long-term outbreak of tracheitis and bronchitis chronic diseases is if untimely maintenance throat also can be that its infection lesion becomes chronic pharyngitis.
The method of treatment chronic pharyngitis has acupuncture operative treatment, laser coking treatment, hot vapour atomizing naturopathy and Chinese and western drugs treatment etc.The biorgan that acupuncture operative treatment and laser therapy damage human body often is that cost is carried out, and carries out methods such as coking processing like the excision tonsil, to diseased region, and operation risk stays hidden danger greatly and easily; Naturopathy such as hot vapour atomizing only play very little assosting effect, poor effect even do not have effect; The doctor trained in Western medicine medication mainly is disinfectant and antibiotic, and this treatment and medication can only alleviate disease to a certain extent, and being difficult to cure should disease, and antibiotic should not be taken for a long time.
Three, summary of the invention:
The method for distilling that the purpose of this invention is to provide the total yellow handle extract of a kind of Herba Salviae Plebeiae, this extract are the Chinese medicine that the treatment chronic pharyngitis has good effects, for the patient who suffers from chronic pharyngitis brings glad tidings.Content of total flavone is high in this common sage herb general flavone extract, and it has the effect of significant treatment pharyngitis.
The objective of the invention is to realize through following technical scheme:
A kind of method for distilling of common sage herb general flavone extract is characterized in that extraction step is following:
1) get 300-500 gram Herba Salviae Plebeiae aerial parts and pulverize after, boil 3 times with the decocting of 10-12 times of medical material amounts, filter the back filtrating concentrate the Herba Salviae Plebeiae concentrated solution;
2) above-mentioned Herba Salviae Plebeiae concentrated solution is passed through pretreated polyamide column;
3) wash above-mentioned polyamide column, use the eluent eluting then, collect solution behind the eluting, and be concentrated into driedly, obtain common sage herb general flavone extract.
The method for distilling of described common sage herb general flavone extract is characterized in that step 2) described in polyamide be 30 ~ 60 orders.
The method for distilling of described common sage herb general flavone extract is characterized in that the eluent described in the step 3) is pure liquid or ketone liquid, and the concentration of eluent is 20 ~ 75%.
The method for distilling of described common sage herb general flavone extract, the flow velocity that it is characterized in that the said washing of step 3) is 5 ~ 7BV/h, the flow velocity of said eluent is 2 ~ 7BV/h.
The application of described common sage herb general flavone extract in preparation treatment chronic pharyngitis disease medicament.
Herba Salviae Plebeiae is a Labiatae salvia Herba Salviae Plebeiae
Salvia plebeia R.BrownHerb, another name snow is seen grass, favus's grass, leprosy group grass, Herba Potentillae Chinensis, elephant skin grass etc., is born on the wetland, except that Xinjiang, Gansu, Qinghai, Tibet, almost is distributed in all parts of the country.These article bitter in the mouth, suffering, cool in nature are returned lung, stomach, kidney channel, have heat-clearing and toxic substances removing, inducing diuresis to remove edema, effects such as cooling blood for hemostasis.Clinically be used to treat acute tonsillitis, chronic bronchitis, vaginitis, cervical erosion, its compound recipe is available treatment acute urinary tract infection etc. also.In recent years; The medical science worker studies more to its pharmacological action, chemical constituent; The result shows, that Herba Salviae Plebeiae has is antibacterial, relieving cough and asthma, eliminate the phlegm and effects such as antihistamine, anti-liver damage, and its chemical constituent is flavonoid, paste type and caffeic acid etc.; The flavonoid chemical constituent is its main component, and flavonoid comprises Homoplantaginin and aglycon thereof, herba lycopi flavone, Nepeta glycosides, eupatorin etc.According to another report, salvia plebeia extract has tangible antioxidation to animal and plant fat; Dinatin, herba lycopi flavone have antiinflammatory action.
Beneficial effect of the present invention is specific as follows:
1, to adopt water that the top of the Herba Salviae Plebeiae of pulverizing is carried out multiple extraction in the present invention, after the filtering and concentrating, and polyamide absorption; Reuse eluent eluting, solution behind the collection eluting, drying obtains common sage herb general flavone extract; Through measuring, general flavone content wherein is greater than more than 80%, and equipment investment is few; With short production cycle, prepare efficient, simple to operate, safety, cost low, be easy to suitability for industrialized production;
2, the common sage herb general flavone extract that obtains of the present invention, it has the effect of significant treatment chronic pharyngitis the pharmacological experiment proof, for the further development and use of Herba Salviae Plebeiae provide the basis.
Four, the specific embodiment
Below in conjunction with specific embodiment, further set forth invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.Except as otherwise noted, otherwise all percentage ratio and umber by weight.
Only if definition separately, the same meaning that employed all specialties and scientific words and one skilled in the art are familiar with in the literary composition.In addition, any with the institute similar content of putting down in writing or the equalization method and material all can be applicable in the inventive method.Preferable implementation method described in the literary composition and the material usefulness that only presents a demonstration.
Embodiment 1
Take by weighing 500 g Herba Salviae Plebeiae aerial partss, dry, pulverize after, add the water extraction 3 times of 5600 mL, each 2 h.Merge extractive liquid, filters polyamide column on the concentrating under reduced pressure;
Get 30 ~ 60 good order polyamide of certain pretreatment, the internal diameter of packing into is in the glass column of 5.4 cm, and making its bed volume is 600 mL, makes the Herba Salviae Plebeiae filtrate slowly pass through polyamide column with the flow velocity of 3 BV/h; Solution residual in the post is removed in the washing of 1200 mL then; To be 75% alcoholic solution pass through resin column with the flow velocity of 3.5 BV/h to reuse concentration; Solution behind the eluting between collection 0.5 ~ 3BV, pressurization is concentrated into dried, obtains the tan powder of 10.5 g behind the drying under reduced pressure; Yield is 3.5% (crude drug meter) common sage herb general flavone extract, and its general flavone content is 89.3%.
Embodiment 2
Take by weighing 500 g Herba Salviae Plebeiae aerial partss, dry, pulverize after, add the water extraction 3 times of 5600 mL, each 2 h.Merge extractive liquid, filters, concentrating under reduced pressure or the concentrated polyamide column of directly going up.
Get 30 ~ 60 good order polyamide of certain pretreatment, the internal diameter of packing into is in the glass column of 4.6 cm, and making its bed volume is 600 mL, makes the Herba Salviae Plebeiae filtrate slowly pass through polyamide column with the flow velocity of 3 BV/h.Solution residual in the post is removed in the washing of 1200 mL then; To be 75% alcoholic solution pass through resin column with the flow velocity of 3.5 BV/h to reuse concentration; Solution behind the eluting between collection 0.5 ~ 3BV, pressurization is concentrated into dried, obtains the tan powder of 10.5 g behind the drying under reduced pressure; Yield is 3.5% (crude drug meter) common sage herb general flavone extract, and its general flavone content is 85.5%.
It is to adopt NaNO that resulting common sage herb general flavone extract in the foregoing description, the content of total flavone that wherein contains are measured
2-Al (NO
3)
3-NaOH complex colorimetry is measured, and is external standard with the rutin, finds that wherein content of total flavone is not less than 80%.
Embodiment 3 pharmacological tests
1 material and reagent
The common sage herb general flavone extract that embodiment 1 obtains; Pharyngitis mouth-sucking tablet (Anhui Ji people medicinal liquid company limited, lot number: 110402); Ammonia (Nanjing Chemistry Reagent Co., Ltd., lot number: 11062430380); 60 of SD rats, male (Zhejiang Province's Experimental Animal Center).
2 experimental techniques
Get 60 SD rats and be divided into 6 groups, complete male, random packet.Be high, medium and low three dose groups of normal control group, model group, positive controls (pharyngitis mouth-sucking tablet) and common sage herb general flavone extract.Hold the back fixation rat in hand, pull out tongue and press down, 15% ammonia of in the throat sprayer unit, packing into stretches into the rat oral cavity to pharyngeal with shower nozzle, pushes spray switch rapidly, and continuous 3 times, make pharyngeal mucosa because of the congested swelling of acute irritation, form acute pharyngitis.Blank control group replaces ammonia with distilled water.Behind modeling 24 h, medicine fully is dissolved in the distilled water, with irritating stomach pin gastric infusion; Model control group and blank group give distilled water; 1 time/d, continuous 3 d, each activities in rats situation, diet drinking-water situation and pharyngeal mucosa finding of naked eye etc. after observation every day and the record modeling.Last administration next day, each organizes rats by intraperitoneal injection 10% chloral hydrate anesthesia (fasting 12 h before the anesthesia), and it is fixing to lie on the back, and exposes pharyngeal cavity; Get pharynx wall and organize 0.5 cm * 0.5 cm, conventional film-making behind the normal saline flushing, above-mentioned histoorgan is through 10% formalin fixed, routine paraffin wax embedding; Slice thick 4～5 μ m, HE dyeing, the pathology professional reads sheet, according to the pathological changes light and heavy degree; Sxemiquantitative is slight "+" successively, moderate " ++ ", and severe " +++", no pathological changes tissue mark is "-".
3 experimental results
Table 1 rat pharynx wall pathological section microscopy situation
Table 1 is the result show, normal control group pharyngeal mucous membrane indumentum stratified squamous epithelium is gone up the subcutaneous loose connective tissue that has, and mucomembranous epithelial cell does not have degeneration, necrosis.Mucous epithelium undertissue does not have hyperemia, edema, it is thus clear that be dispersed in indivedual cell infiltration, the mucus body of gland is high-visible; The 5/10 routine pharyngeal mucous membrane epithelial cell degeneration (+) of model group rat; Congested, the edema (++) of loose connective tissue under the mucosa; Cell infiltration (++) is surplused congested, the edema (+) of loose connective tissue under the 5/10 routine pharyngeal mucous membrane mucous epithelium, cell infiltration (+); The pharynx mucosa hyperemia of each dose groups of common sage herb general flavone extract, edema, inflammatory cell are invaded the profit situation all to be had in various degree and alleviates.
Conclusion: after the modeling of rat ammonia, pharyngeal mucous membrane hyperemia, edema all appear, cell infiltration, and after rat gave common sage herb general flavone extract, above-mentioned symptom obviously reduces, and was particularly evident with Herba Salviae Plebeiae total flavones high dose group.
Embodiment 4 pharmacological testings
1 material and reagent
The common sage herb general flavone extract that embodiment 2 obtains; Aspirin Enteric-coated Tablets (Bayer HealthCare Co, lot number: BJ02895); 50 of the big mices of ICR, male (Yangzhou University comparative medicine center).
2 experimental techniques
50 of ICR mices are divided into 5 groups at random, do not organize 10, i.e. high, medium and low three dose groups of normal control group, model group, positive controls (Aspirin Enteric-coated Tablets) and common sage herb general flavone extract.Below respectively organize gastric infusion (the normal control group replaces with distilled water), 1 time/d, continuous 6 d are behind last administration 1 h; 50 μ L/ only are applied to the mouse right ear two sides with xylene, and left ear is not coated with as contrast, cause mice to be taken off cervical vertebra behind scorching 30 min and put to death; Cut two ears along the auricle baseline, lay round auricle at the same position of left and right ear respectively, weigh with the card punch of diameter 9 mm; Ask the poor of left and right two ear weight,, be calculated as follows the swelling rate as the swelling degree:
Ear swelling rate=(auris dextra weight-left ear weight)/left ear weight * 100%
Ear swelling suppression ratio %=(the average ear swelling degree of the average ear swelling degree one administration group of blank control group) the average ear swelling degree of ÷ blank control group * 100%
3 experimental results
With each administration group swelling rate of normal control group comparison downward trend is arranged, and dose groups in the Aspirin Enteric-coated Tablets, Herba Salviae Plebeiae total flavones high dose group, Herba Salviae Plebeiae total flavones (P 0.01, P 0.05) see table 2
| Group | Dosage (g/kg) | Swelling degree/mg |
| Normal saline | - | 16.0±6.2082 |
| Aspirin | 0.033 | 6.54±4.1121** |
| The Herba Salviae Plebeiae high dose group | 20 | 8.87±1.6545** |
| Dose groups in the Herba Salviae Plebeiae | 10 | 9.23±4.5453* |
| The Herba Salviae Plebeiae low dose group | 5 | 13.8±6.0531 |
Compare with the normal control group, * * P 0.01, * P < 0.05
Conclusion: dose groups has the swelling rate effect that reduces ear swelling due to the mice xylene in Aspirin Enteric-coated Tablets, Herba Salviae Plebeiae total flavones high dose group, the Herba Salviae Plebeiae total flavones, and promptly they have antiinflammatory action.
In sum, general flavone content is high in the common sage herb general flavone extract of the present invention, has the effect of significant treatment pharyngitis, and has certain antiinflammatory action, prepare efficient, simple to operate, safety, cost low, be easy to suitability for industrialized production.
Claims (5)
1. the method for distilling of a common sage herb general flavone extract is characterized in that extraction step is following:
1) get 300-500 gram Herba Salviae Plebeiae aerial parts and pulverize after, boil 3 times with the decocting of 10-12 times of medical material amounts, filter the back filtrating concentrate the Herba Salviae Plebeiae concentrated solution;
2) above-mentioned Herba Salviae Plebeiae concentrated solution is passed through pretreated polyamide column;
3) wash above-mentioned polyamide column, use the eluent eluting then, collect solution behind the eluting, and be concentrated into driedly, obtain common sage herb general flavone extract.
2. the method for distilling of common sage herb general flavone extract according to claim 1 is characterized in that step 2) described in polyamide be 30 ~ 60 orders.
3. the method for distilling of common sage herb general flavone extract according to claim 1 is characterized in that the eluent described in the step 3) is pure liquid or ketone liquid, and the concentration of eluent is 20 ~ 75%.
4. the method for distilling of common sage herb general flavone extract according to claim 1, the flow velocity that it is characterized in that the said washing of step 3) is 5 ~ 7BV/h, the flow velocity of said eluent is 2 ~ 7BV/h.
5. the application of common sage herb general flavone extract as claimed in claim 1 in preparation treatment chronic pharyngitis disease medicament.
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| CN109998012A (en) * | 2019-05-21 | 2019-07-12 | 平顶山学院 | Common Sage Herb general flavone extracts and the method for general flavone solid beverage preparation |
| CN110621326A (en) * | 2017-05-11 | 2019-12-27 | Kt & G 株式会社 | Composition for preventing or treating respiratory inflammation comprising combined herb extracts of Salvia plebeia and Red Ginseng as active ingredients and use thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110621326A (en) * | 2017-05-11 | 2019-12-27 | Kt & G 株式会社 | Composition for preventing or treating respiratory inflammation comprising combined herb extracts of Salvia plebeia and Red Ginseng as active ingredients and use thereof |
| CN110621326B (en) * | 2017-05-11 | 2022-03-25 | Kt & G 株式会社 | Pharmaceutical composition for preventing or treating respiratory inflammation and application thereof |
| US11510955B2 (en) | 2017-05-11 | 2022-11-29 | Kt&G Corporation | Composition comprising a combined herb extract of Salvia plebia and red ginseng as active ingredients for preventing or treating a respiratory inflammation and the use thereof |
| CN109998012A (en) * | 2019-05-21 | 2019-07-12 | 平顶山学院 | Common Sage Herb general flavone extracts and the method for general flavone solid beverage preparation |
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Application publication date: 20120912 |