CN109998012A - Common Sage Herb general flavone extracts and the method for general flavone solid beverage preparation - Google Patents
Common Sage Herb general flavone extracts and the method for general flavone solid beverage preparation Download PDFInfo
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- CN109998012A CN109998012A CN201910423437.9A CN201910423437A CN109998012A CN 109998012 A CN109998012 A CN 109998012A CN 201910423437 A CN201910423437 A CN 201910423437A CN 109998012 A CN109998012 A CN 109998012A
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- Prior art keywords
- common sage
- sage herb
- general flavone
- polyamide
- flavone
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- 229930003944 flavone Natural products 0.000 title claims abstract description 85
- 235000011949 flavones Nutrition 0.000 title claims abstract description 85
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- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 77
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 73
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- 238000002360 preparation method Methods 0.000 title claims abstract description 11
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Common Sage Herb total flavone extracting method, comprising the following steps: (1) choosing fresh lichee grass is raw material, dry, crushing, sieving；(2) it impregnates, is ultrasonically treated in ethanol；(3) it extracts, obtains Common Sage Herb crude extract；(4) preparation absorption resin；(5) resin adsorption Common Sage Herb crude extract；(6) it elutes, be dried to obtain Common Sage Herb flavones；(7) standard curve is drawn, sample general flavone average content is calculated；(8) determination oxidative is carried out to general flavone.The present invention has operating method simple, improves Common Sage Herb general flavone and extracts content, and quality greatly improves (including purity is high, dissolubility and good dispersion), prepares solid beverage with the Common Sage Herb general flavone, helps to improve the economic value of Common Sage Herb.
Description
Technical field
The invention belongs to natural medicinal plant effective component extracting technical fields, and in particular to a kind of Common Sage Herb general flavone mentions
Take and the general flavone solid beverage preparation method.
Background technique
Common Sage Herb is the herb of Lamiaceae Salvia platymiscium Common Sage Herb first recorded in Compendium of Material Medica, also known as snow is shown in grass, leprosy
Bothriospermum Chinese, green snow, Lai Zicao, leprosy group grass, shepherd's purse peaceful, pig mother-in-law grass, elephant skin grass etc..It is cool in nature, acrid flavour, hardship, return lung, stomach, kidney channel, clearly
Thermal detoxification, inducing diuresis to remove edema, cooling blood and hemostasis, for treat hemoptysis, haematemesis, hematuria, metrorrhagia and metrostaxis, ascites, gonorrhoea, abscess of throat, carbuncle swells,
Hemorrhoid etc..Common Sage Herb is biennial upright draft.China is distributed in addition to Xinjiang, Gansu, Qinghai, Tibet, it is resourceful and
It is cheap, it is also applied to the industries such as agricultural, health care product in recent years, has a vast market development prospect.Flavonoid
Object is the ingredient that content is more in Common Sage Herb, has the pharmacological activity such as significant anti-oxidant, liver protection.The structure of flavone compound
And classification flavone compound is a kind of substance also known as bioflavonoids widely distributed in nature, is chromone
Or the derivative of chromogen alkane, it is the natural products with a structure for basic parent nucleus.It, can be by such according to the difference of mother nucleus structure
Compound is divided into flavones and flavonols, such as apiolin, canopy skin element, myricetin flavanones and flavanones alcohols, as farrerol is looked into
Ear ketone, such as safflower former times anthocyan, such as delphinidin osajin, such as Puerarin.In addition, there are also aurones classes, bisflavones
With the Flavonoid substances of fraction other structures.Flavone compound have remove free radical, it is anti-oxidant, it is anti-mutation, it is antitumor,
It is antibacterial, antiviral and adjust the functions such as immune.
Claim in " Tian Bao book on Chinese herbal medicine " Common Sage Herb " specially control cough, disappear lung Jin Huosheng ", civil people often take Common Sage Herb decoction or
It treats and coughs as vegetable edible.Common Sage Herb can obviously increase sputum in the phenol red discharge rate of mouse breathing mucous membrane and rats breathing road
Secretory volume, extend mouse hidden cough incubation period, reduce cough number.Common Sage Herb can inhibit in RAW264.7 cell proinflammatory because
The generation of son such as tumor necrosis factor (TNF)-α, interleukins (IL) -1 and IL-6, wherein aerial part inhibiting effect
It is more stronger than the effect of root.Using ovalbumin induced asthma mouse model, it is found that Common Sage Herb aerial part can significantly reduce mouse
Airway eosinophilia's quantity reduces mucilage secretion and inflammatory cell infiltration, can be effectively improved asthma mice lung tissue
Pathological change acts on close with dexamethasone.The report of Common Sage Herb antioxidant activity research in recent years is more, main antioxidant activity
Ingredient is flavone compound.The active constituent that Common Sage Herb plays hepatoprotective effect is flavonoids, and mechanism of action mainly passes through antioxygen
Change damage, inhibit inflammatory factor release etc..
Protective effect of the flavones ingredient Homoplantaginin to liver cell in Common Sage Herb, flavonoids can significantly reduce in Common Sage Herb
Lactate dehydrogenase levels increase glutathione, glutathione peroxidase and superoxide dismutase content, experiment in vivo
In, cause liver injury model discovery Homoplantaginin to can inhibit alanine aminase and asparagus fern in serum using bacillus and lipopolysaccharides
The increase of propylhomoserin transferase content, it is possible to reduce the content of thiobarbituric acid reaction object in hepatic tissue homogenate.
Currently, the preparation method to Common Sage Herb general flavone uses following steps:
A. Common Sage Herb herb is spontaneously dried, and crushed 80 meshes later；
B. a certain amount of Common Sage Herb powder is weighed, Common Sage Herb powder and ethyl alcohol are uniformly mixed by 1:3 in proportion；
C. ultrasonic wave extraction, 20 DEG C of temperature, time 12h；
D. it obtains liquid and carries out vacuum pump filtering, centrifuge separation；
E. the temperature of liquid electric heating thermostatic drying chamber is 60 DEG C, and Common Sage Herb flavones drying and processing is carried out to it.
The content that above-mentioned this method extracts general flavone is lower, purity is not high.The disadvantages of dissolubility and bad dispersibility.
Summary of the invention
The present invention provides a kind of total yellow convenient for operation, simple process, extraction to solve shortcoming in the prior art
High, the with high purity Common Sage Herb general flavone of ketone content extracts and the method for general flavone solid beverage preparation.
In order to solve the above technical problems, the present invention adopts the following technical scheme: Common Sage Herb total flavone extracting method, including with
Lower step,
(1) choosing fresh lichee grass is raw material, dry, crushing, sieving；
(2) it impregnates, is ultrasonically treated in ethanol；
(3) it extracts, obtains Common Sage Herb crude extract；
(4) preparation absorption resin；
(5) resin adsorption Common Sage Herb crude extract；
(6) it elutes, be dried to obtain Common Sage Herb flavones；
(7) standard curve is drawn, sample general flavone average content is calculated；
(8) determination oxidative is carried out to general flavone.
Step (1) and (2) are specially including following process: by the whole strain grass freeze-drying of fresh lichee grass, crushing, crossing 100
Mesh；5 parts of Common Sage Herb powder, every part of 60g are taken, is added separately in 6 times of 95% alcohol solvents of weight, 12h is impregnated；To mixed liquor
Progress ultrasonic wave extraction, 4 DEG C of temperature, time 12h.
Step (3) is specially including following process: decompression suction filtration, petroleum ether extraction and ethyl acetate equal-volume extraction are abandoned
Ether phase stays water phase, obtains Common Sage Herb crude extract.
Step (4) is specially including following process: the certain polyamide of electronic balance weighing places it in beaker,
With soaked in absolute ethyl alcohol 48h, sufficiently dissolution and swelling state are reached；Resin after immersion is filtered dry with small slip through the net in aperture, then
With a large amount of distilled water flushings, the color of trickle is checked, until not having white opacity, then continued with a large amount of distilled water
Resin layer is rinsed, up to no alcohol taste, it is spare to take out resin.
Step (5) is specially including following process:
A. the 100-200 each 2g of mesh polyamide pre-processed is taken, is respectively placed in the conical flask of 300mL；It is measured with graduated cylinder
The Common Sage Herb crude extract of 60mL is added in bottle in step d, after film seal, places it in constant temperature oscillator, 20 DEG C of upper vibrations
48h is swung, it is noted that shading treatment filters stand-by after its completely absorption in oscillatory process；
B. on the basis of having carried out Staticadsorption experiment, with the used polyamide of a large amount of distilled water flushings, until stream
Liquid is in colourless out, then blots the remaining sample liquid of resin surface with filter paper；By carrying out above-mentioned steady-state solution adsorption experiment, than
To the analysis adsorbance of polyamide, desorption quantity, adsorption rate and desorption efficiency four indexs；
C. polyamide that is selected, handling well being fitted into glass chromatography column, the glass internal diameter of chromatographic column is 1cm,
During filling column, polyamide can reduce the generation of bubble with the state entrance soaked, using absorbent cotton, by glass column
Both ends stopper, and the impact force that can prevent polyamide particle from generating by pouring into for sample liquid in this way causes it to float on layer
It analyses in column；
When d. carrying out adsorption experiment under normal temperature environment, general flavone aqueous extract is fitted into chromatographic column, by controlling applied sample amount
With the ratio of polyamide and the pH value of sample solution the two influence factors, using polyamide adsorption rate as test index into
Mobile state adsorption experiment, to obtain optimal resin adsorption conditions.
Step (6) is specially including following process: eluent uses alcohol liquid, and the concentration of eluent is 30-60%, elution point
From general flavone is obtained, dry to general flavone in the Constant Temp. Oven that temperature is 60 DEG C, then -20 DEG C of freezen protectives are standby
With.
Step (2) concrete operations process is the flavonoid standards 0.2g for weighing 120 DEG C of dry constant weights, adds dehydrated alcohol molten
It solves and is settled in 100mL volumetric flask, shake up, the standard solution that concentration is 0.2 mg/mL is made；Standard solution is taken respectively
0.2 m L, 0.4 mL, 0.8 mL, 1.6 mL, 2.0 mL add 5 % sodium nitrite 0.3mL mixed in five 10 mL volumetric flasks
It is even, place 6 min after, add 10% aluminum nitrate, 0.3 mL, mix, place 6min, then plus 4% sodium hydroxide 4mL, add redistilled water
It to scale, shakes up, places 15min, carry out full wavelength scanner, measure has absorption maximum at 510nm；Compare light absorption value knot
Fruit draws standard curve；By standard curve, sample Common Sage Herb general flavone average content is calculated.
Step (3) concrete operations process is to prepare 6 clean test tube with ground stoppers, takes a series of various concentration ladders of 5mL respectively
The sample prepare liquid of degree is added thereto, then the H that 5mL concentration is 10mmol/L is added thereto2O2Solution, H2O2The pH of solution=
7.4, it covers tightly test tube plug and sufficiently shakes up；Its absorbance is measured at 248nm；The clearance rate value of absorbance is higher, shows antioxygen
Change effect is stronger；Conversely, then oxidation resistance is poorer.
The method that general flavone prepares general flavone solid beverage takes a certain amount of general flavone, addition white granulated sugar, citric acid and wood
Sugar alcohol obtains general flavone brown sugar solid beverage after mixing thoroughly.
The method that the general flavone of extraction prepares general flavone solid beverage takes a certain amount of general flavone, adds white granulated sugar, malt
Dextrin and cyclodextrine obtain general flavone brown sugar solid beverage after mixing thoroughly.
By adopting the above technical scheme, the invention has the following advantages:
Step (1) and (2): freeze-drying reduces composition deactivation；
Step (3): other compositions pollution in extraction process is reduced；
Step (4)-(6): the separation purity of Common Sage Herb flavones is promoted.
In conclusion the present invention has operating method simple, improves Common Sage Herb general flavone and extract content, and quality is significantly
It improves (including purity is high, dissolubility and good dispersion), prepares solid beverage with the Common Sage Herb general flavone, help to improve lichee
The economic value of grass.
Specific embodiment
Common Sage Herb total flavone extracting method, includes the following steps,
(1) choosing fresh lichee grass is raw material, dry, crushing, sieving；
(2) it impregnates, is ultrasonically treated in ethanol；
(3) it extracts, obtains Common Sage Herb crude extract；
(4) preparation absorption resin；
(5) resin adsorption Common Sage Herb crude extract；
(6) it elutes, be dried to obtain Common Sage Herb flavones；
(7) standard curve is drawn, sample general flavone average content is calculated；
(8) determination oxidative is carried out to general flavone.
Step (1) and (2) are specially including following process: by the whole strain grass freeze-drying of fresh lichee grass, crushing, crossing 100
Mesh；5 parts of Common Sage Herb powder, every part of 60g are taken, is added separately in 6 times of 95% alcohol solvents of weight, 12h is impregnated；To mixed liquor
Progress ultrasonic wave extraction, 4 DEG C of temperature, time 12h.
Step (3) is specially including following process: decompression suction filtration, petroleum ether extraction and ethyl acetate equal-volume extraction are abandoned
Ether phase stays water phase, obtains Common Sage Herb crude extract.
Step (4) is specially including following process: the certain polyamide of electronic balance weighing places it in beaker,
With soaked in absolute ethyl alcohol 48h, sufficiently dissolution and swelling state are reached；Resin after immersion is filtered dry with small slip through the net in aperture, then
With a large amount of distilled water flushings, the color of trickle is checked, until not having white opacity, then continued with a large amount of distilled water
Resin layer is rinsed, up to no alcohol taste, it is spare to take out resin.
Step (5) is specially including following process:
A. the 100-200 each 2g of mesh polyamide pre-processed is taken, is respectively placed in the conical flask of 300mL；It is measured with graduated cylinder
The Common Sage Herb crude extract of 60mL is added in bottle in step d, after film seal, places it in constant temperature oscillator, 20 DEG C of upper vibrations
48h is swung, it is noted that shading treatment filters stand-by after its completely absorption in oscillatory process；
B. on the basis of having carried out Staticadsorption experiment, with the used polyamide of a large amount of distilled water flushings, until stream
Liquid is in colourless out, then blots the remaining sample liquid of resin surface with filter paper；By carrying out above-mentioned steady-state solution adsorption experiment, than
To the analysis adsorbance of polyamide, desorption quantity, adsorption rate and desorption efficiency four indexs；
C. polyamide that is selected, handling well being fitted into glass chromatography column, the glass internal diameter of chromatographic column is 1cm,
During filling column, polyamide can reduce the generation of bubble with the state entrance soaked, using absorbent cotton, by glass column
Both ends stopper, and the impact force that can prevent polyamide particle from generating by pouring into for sample liquid in this way causes it to float on layer
It analyses in column；
When d. carrying out adsorption experiment under normal temperature environment, general flavone aqueous extract is fitted into chromatographic column, by controlling applied sample amount
With the ratio of polyamide and the pH value of sample solution the two influence factors, using polyamide adsorption rate as test index into
Mobile state adsorption experiment, to obtain optimal resin adsorption conditions.
Step (6) is specially including following process: eluent uses alcohol liquid, and the concentration of eluent is 30-60%, elution point
From general flavone is obtained, dry to general flavone in the Constant Temp. Oven that temperature is 60 DEG C, then -20 DEG C of freezen protectives are standby
With.
Step (2) concrete operations process is the flavonoid standards 0.2g for weighing 120 DEG C of dry constant weights, adds dehydrated alcohol molten
It solves and is settled in 100mL volumetric flask, shake up, the standard solution that concentration is 0.2 mg/mL is made；Standard solution is taken respectively
0.2 m L, 0.4 mL, 0.8 mL, 1.6 mL, 2.0 mL add 5 % sodium nitrite 0.3mL mixed in five 10 mL volumetric flasks
It is even, place 6 min after, add 10% aluminum nitrate, 0.3 mL, mix, place 6min, then plus 4% sodium hydroxide 4mL, add redistilled water
It to scale, shakes up, places 15min, carry out full wavelength scanner, measure has absorption maximum at 510nm；Compare light absorption value knot
Fruit draws standard curve；By standard curve, sample Common Sage Herb general flavone average content is calculated.
Step (3) concrete operations process is to prepare 6 clean test tube with ground stoppers, takes a series of various concentration ladders of 5mL respectively
The sample prepare liquid of degree is added thereto, then the H that 5mL concentration is 10mmol/L is added thereto2O2Solution, H2O2The pH of solution=
7.4 covering tightly test tube plug and sufficiently shaking up；Its absorbance is measured at 248nm；The clearance rate value of absorbance is higher, shows antioxygen
Change effect is stronger；Conversely, then oxidation resistance is poorer.
Using the Common Sage Herb general flavone of distinct methods preparation in the present embodiment and background technique, general flavone generates content ratio
Compared with the present embodiment effect is more preferable in contrast, specifically see the table below.
It is clear for Common Sage Herb general flavone using the Common Sage Herb general flavone of distinct methods preparation in the present embodiment and background technique
Except rate, the present embodiment effect is more preferable in contrast, specifically see the table below.
By taking the general flavone of the extraction of the present embodiment as an example, it is solid that the general flavone that the present invention is extracted from Common Sage Herb prepares general flavone
The method of body beverage, including following two embodiment:
Embodiment one: Common Sage Herb general flavone additive amount is respectively 0.2,0.4,0.6,0.8,1.0 g, five seed type, is added white
Granulated sugar 0.2g, citric acid add 0.2g, and it is 3g that five kinds of Common Sage Herb general flavone solid beverage total weights are supplied after xylitol addition.
Embodiment two: Common Sage Herb general flavone additive amount is respectively 0.2,0.4,0.6,0.8,1.0 g, adds white granulated sugar
0.2g, maltodextrin 0.2g, it is 3g that five kinds of Common Sage Herb general flavone solid beverage total weights are supplied after cyclodextrine addition.
Distinct methods Common Sage Herb general flavone solid beverage sensory evaluation scores, the effect of embodiment one is more preferable in contrast, tool
Body see the table below.
The present embodiment not makes any form of restriction shape of the invention, material, structure etc., all according to this hair
Bright technical spirit any simple modification, equivalent change and modification to the above embodiments, belong to the technology of the present invention side
The protection scope of case.
Claims (10)
1. Common Sage Herb total flavone extracting method, it is characterised in that: include the following steps,
(1) choosing fresh lichee grass is raw material, dry, crushing, sieving；
(2) it impregnates, is ultrasonically treated in ethanol；
(3) it extracts, obtains Common Sage Herb crude extract；
(4) preparation absorption resin；
(5) resin adsorption Common Sage Herb crude extract；
(6) it elutes, be dried to obtain Common Sage Herb flavones；
(7) standard curve is drawn, sample general flavone average content is calculated；
(8) determination oxidative is carried out to general flavone.
2. Common Sage Herb total flavone extracting method according to claim 1, it is characterised in that: step (1) and (2) are specially wrapped
It includes following process: the whole strain grass of fresh lichee grass being freeze-dried, crush, is sieved with 100 mesh sieve；Take 5 parts of Common Sage Herb powder, every part
60g is added separately in 6 times of 95% alcohol solvents of weight, impregnates 12h；To mixed liquor carry out ultrasonic wave extraction, 4 DEG C of temperature, when
Between 12h.
3. Common Sage Herb total flavone extracting method according to claim 2, it is characterised in that: step (3) be specially include with
Lower process: decompression suction filtration, petroleum ether extraction and ethyl acetate equal-volume extraction abandon ether phase, stay water phase, obtain Common Sage Herb coarse extraction
Liquid.
4. Common Sage Herb total flavone extracting method according to claim 3, it is characterised in that: step (4) be specially include with
Lower process: the certain polyamide of electronic balance weighing places it in beaker, with soaked in absolute ethyl alcohol 48h, reaches and fill
Divide dissolution and swelling state；Resin after immersion is filtered dry with small slip through the net in aperture, then with a large amount of distilled water flushings, checks trickle
Color then continued to rinse resin layer with a large amount of distilled water until there is no white opacity, until no alcohol taste, take out and set
Rouge is spare.
5. Common Sage Herb total flavone extracting method according to claim 4, it is characterised in that: step (5) be specially include with
Lower process:
A. the 100-200 each 2g of mesh polyamide pre-processed is taken, is respectively placed in the conical flask of 300mL；It is measured with graduated cylinder
The Common Sage Herb crude extract of 60mL is added in bottle in step d, after film seal, places it in constant temperature oscillator, 20 DEG C of upper vibrations
48h is swung, it is noted that shading treatment filters stand-by after its completely absorption in oscillatory process；
B. on the basis of having carried out Staticadsorption experiment, with the used polyamide of a large amount of distilled water flushings, until stream
Liquid is in colourless out, then blots the remaining sample liquid of resin surface with filter paper；By carrying out above-mentioned steady-state solution adsorption experiment, than
To the analysis adsorbance of polyamide, desorption quantity, adsorption rate and desorption efficiency four indexs；
C. polyamide that is selected, handling well being fitted into glass chromatography column, the glass internal diameter of chromatographic column is 1cm,
During filling column, polyamide can reduce the generation of bubble with the state entrance soaked, using absorbent cotton, by glass column
Both ends stopper, and the impact force that can prevent polyamide particle from generating by pouring into for sample liquid in this way causes it to float on layer
It analyses in column；
When d. carrying out adsorption experiment under normal temperature environment, general flavone aqueous extract is fitted into chromatographic column, by controlling applied sample amount
With the ratio of polyamide and the pH value of sample solution the two influence factors, using polyamide adsorption rate as test index into
Mobile state adsorption experiment, to obtain optimal resin adsorption conditions.
6. Common Sage Herb total flavone extracting method according to claim 5, it is characterised in that: step (6) be specially include with
Lower process: eluent uses alcohol liquid, and the concentration of eluent is 30-60%, elutes isolated general flavone, is 60 DEG C in temperature
Dry to general flavone in Constant Temp. Oven, then -20 DEG C of freezen protectives are spare.
7. Common Sage Herb total flavone extracting method according to claim 6, it is characterised in that: step (2) concrete operations process
To weigh the flavonoid standards 0.2g of 120 DEG C of dry constant weights, adding dehydrated alcohol to dissolve and be settled in 100mL volumetric flask, shake
It is even, the standard solution that concentration is 0.2 mg/mL is made；0.2 m L of standard solution, 0.4 mL, 0.8 mL, 1.6 are taken respectively
ML, 2.0 mL add 5 % sodium nitrite 0.3mL to mix in five 10 mL volumetric flasks, after placing 6 min, add 10% aluminum nitrate
0.3 mL is mixed, and places 6min, then plus 4% sodium hydroxide 4mL, add redistilled water to shake up to scale, place 15min, carry out
Full wavelength scanner, measure has absorption maximum at 510nm；Light absorption value is compared as a result, drawing standard curve；Pass through standard song
Line calculates sample Common Sage Herb general flavone average content.
8. Common Sage Herb total flavone extracting method according to claim 7, it is characterised in that: step (3) concrete operations process
For, prepare 6 clean test tube with ground stoppers, a series of sample prepare liquid of various concentration gradients of 5mL is taken to be added thereto respectively, then to
The H that 5mL concentration is 10mmol/L is wherein added2O2Solution, H2O2PH=7.4 of solution cover tightly test tube plug and sufficiently shake up；In
Its absorbance is measured at 248nm；The clearance rate value of absorbance is higher, shows that antioxidation is stronger；Conversely, then anti-oxidant energy
Power is poorer.
9. the method for preparing general flavone solid beverage using the general flavone that claim 1 is extracted, it is characterised in that: take a certain amount of
General flavone, addition white granulated sugar, citric acid and xylitol, obtains general flavone brown sugar solid beverage after mixing thoroughly.
10. the method for preparing general flavone solid beverage using the general flavone that claim 1 is extracted, it is characterised in that: take a certain amount of
General flavone, addition white granulated sugar, maltodextrin and cyclodextrine obtain general flavone brown sugar solid beverage after mixing thoroughly.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113694080A (en) * | 2021-08-17 | 2021-11-26 | 南京晓庄学院 | Antiviral application of common sage herb polysaccharide |
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| CN102657698A (en) * | 2012-05-31 | 2012-09-12 | 南京中医药大学 | Extraction method and application of salvia plebeia general flavone extract |
| KR20130109336A (en) * | 2012-03-27 | 2013-10-08 | 홍영표 | Sweet rice drink manufacturing method using gombo-baechu |
| CN109452515A (en) * | 2018-12-18 | 2019-03-12 | 李茂刚 | The preparation method of Common Sage Herb honeysuckle compound beverage |
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2019
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| KR20130109336A (en) * | 2012-03-27 | 2013-10-08 | 홍영표 | Sweet rice drink manufacturing method using gombo-baechu |
| CN102657698A (en) * | 2012-05-31 | 2012-09-12 | 南京中医药大学 | Extraction method and application of salvia plebeia general flavone extract |
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Application publication date: 20190712 |