CN105998412A - Antialcoholism formula and preparation method thereof - Google Patents
Antialcoholism formula and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention relates to the field of food and health-care products and particularly discloses an antialcoholism formula and a preparation method thereof. The antialcoholism formula comprises components in parts by weight as follows: 2-10 parts of hovenia acerba extract, 1-8 parts of kudzuvine root extract, 30-50 parts of hawthorn fruit powder, 5-15 parts of vine tea extract and 5-15 parts of propolis extract. The formula has the function of adjusting the viscera through reasonable matching and comprehensive utilization of methods for clearing away heat and toxic materials, promoting the circulation of qi, inducing diuresis, repairing liver injury and the like, further has the advantages of good nourishing, health care and antialcoholism effects and the like due to propolis with higher nutritional value, can effectively relieve alcoholic allergy, headache after drinking, acid stomach and other discomfort symptoms and also repair the liver injury caused by long-time drinking and doesn't have any toxic or side effects.
Description
Technical field
The invention belongs to food, field of health care products, be specifically related to one and relieve the effect of alcohol prescription and preparation method thereof.
Background technology
Spirits culture, as a kind of special form of culture, has the status of its uniqueness in traditional Chinese culture.Thousand of
In the civilized history in year, wine almost penetrates into the every field in social life.People's responsible drinking, to healthy be helpful
, but crapulent a series of malaise symptoms will be produced during insobriety.Also have the biggest a collection of crowd, just somewhat drink
The symptom of alcohol intolerance can be produced, such as skin pruritus, eczema, urticaria, dizziness, nausea,vomiting,diarrhea, even a few peoples also
Anaphylactic shock can occur, and returning its reason is to lack the external skin allergy of one that acetaldehyde invertase (ALDH) causes in human body
Shape.
Liver is important alcoholic intoxication organ, and ethanol ethanol dehydrogenase (ADH) in liver is catalyzed and generates acetaldehyde, second
Aldehyde aldehyde dehydrogenase (ALDH) in liver mitochondrion is further oxidized to nontoxic acetic acid, ultimately generates carbon dioxide and energy
Amount.Liver can have a decomposition to a certain amount of ethanol under normal circumstances, but after long-term or heavy drinking, ethanol is divided by liver
Solution ability is the most not enough.Meanwhile, the metabolic enzyme of liver secretion relatively lags behind again in the absorption of ethanol, therefore, is divided completely at ethanol
Xie Qian, causes damage to liver and brain, and meanwhile, the alcoholic intoxication ability of the liver sustained damage is affected.Therefore, for long-term big
From the point of view of the crowd that amount is drunk, the alcoholic intoxication ability relying solely on liver is inadequate, also to rely on external material to remove ethanol
Poison, reduces the harm to human body of the ethanol poison.Therefore, solution that is a kind of that can assist quick relieving alcoholism and protecting the liver, that have no side effect is developed
Wine product is significant.
At present, disintoxicating product on the market is the beverage made with Sobering-up Chinese medicine material mostly, such as Chinese invention patent CN
The 103750480 open a kind of beverage with hangover-alleviatingfunction function of B, this drinks mainly by Bulbus Lilii, Radix Puerariae, Rhizoma Polygonati Odorati, Poria, fresh Rhizoma Imperatae,
Fresh Rhizoma Phragmitis, white sugar, Mel are mixed in proportion with water and make；The open a kind of Antialcoholic tea of Chinese patent CN 101032267 B, should
Antialcoholic tea is mainly become prescription to make by 10 taste Chinese drug-treated group such as Folium Camelliae sinensis, Folium Kaki, Rhizoma Curcumae Longae, Ganoderma, Radix Salviae Miltiorrhizaes；Chinese patent CN
The 101401603 open a kind of Antialcoholic liver-protecting tea of B, are that to select Flos Chrysanthemi, Japanese raisintree fruit, Flos puerariae lobatae, Radix Puerariae, Pericarpium Citri Reticulatae, Folium Camelliae sinensis be raw material, press
Certain ratio Preparation process forms.But, these beverage for dispelling alcoholic intoxication the most only have effect of removing dampness of relieving the effect of alcohol, heat-clearing and toxic substances removing, right
In alcohol intolerance or the hepatic injury that causes because of insobriety, can not repair, and beverage is inconvenient to carry.
Summary of the invention
For above-mentioned weak point of the prior art, it is an object of the invention to provide one and relieve the effect of alcohol prescription.Described solution
Wine prescription solves existing disintoxicating product and without repairing effect and alcohol intolerance is inconvenient to the problem carried with hepatic injury.
Another object of the present invention is to provide the preparation method of a kind of above-mentioned prescription that relieves the effect of alcohol.
The above-mentioned purpose of the present invention is achieved by the following technical programs.
One is relieved the effect of alcohol prescription, including each component of following parts by weight: Semen Hoveniae (Fructus Hoveniae) extract 2 ~ 10 parts, Radix Puerariae extract 1 ~
8 parts, Fructus Crataegi powder 30 ~ 50 parts, Ampelopsis grossedentata extrat 5 ~ 15 parts, propolis extract 5 ~ 15 parts.
Relieve the effect of alcohol Semen Hoveniae (Fructus Hoveniae) in prescription, Radix Puerariae extract of the present invention has that solving wakes up quenches the thirst and arrange effect of alcoholism, and Ampelopsis grossedentata, propolis carry
Taking thing and have hepatoprotective, anti-hepatic fibrosis, effect of reparation hepatic injury, Fructus Crataegi has the effect of strengthening the spleen and stomach, promoting the circulation of QI to induce diuresis, with
Time can be with the health of facilitating digestion system, the symptom such as gastrointestinal upset that alleviating drinks brings and headache.The present invention is in group of relieving the effect of alcohol
Fang Zhong, with the addition of the Ampelopsis grossedentata in addition to conventional Sobering-up Chinese medicine material, propolis, and by each component reasonable compatibility, plays its cooperative effect,
This disintoxicating product is made to be possible not only to the stomach function regulating that relieves the effect of alcohol, it is also possible to hepatoprotective, reparation hepatic injury.
Preferably, described Semen Hoveniae (Fructus Hoveniae) extract prepares in the following way: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, by solid-liquid ratio 1:(5 ~
7) add the ethanol solution that volume fraction is 70 ~ 90 %, reflux, extract, 1 ~ 3 h at 70 ~ 90 DEG C, extract 2 ~ 3 times；Filter, close
And filtrate, it is evaporated to without alcohol taste；With the ethanol solution precipitate with ethanol that volume fraction is 95%, stand overnight in 4 DEG C；Repetitive operation,
Merging the molten part of alcohol, concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, extracts by ethyl acetate, decompression
Concentrate extraction solution, vacuum drying, obtain acetic acid ethyl ester extract extractum, be Semen Hoveniae (Fructus Hoveniae) extract, standby.Preferably, described dry
40 mesh sieves are crossed after Semen Hoveniae (Fructus Hoveniae) raw material pulverizing.Preferably, described repetitive operation refers to be repeated 2 times.
Preferably, described Radix Puerariae extract prepares in the following way: by Radix puerariae root raw material pulverizing, according to solid-liquid ratio 1:
(10 ~ 15) add the disodium hydrogen phosphate-citrate buffer solution of pH 3.6 ~ 4.5, and being subsequently adding mass concentration is 0.3 ~ 0.5 %
Cellulase, extract after 50 ~ 70 min at 40 ~ 50 DEG C, extracting solution filtered, concentrating under reduced pressure obtains extractum, is Radix Puerariae and carries
Take thing, standby.Preferably, 40 mesh sieves are crossed after described Radix puerariae root raw material pulverizing.
Preferably, described Fructus Crataegi powder prepares in the following way: is dried at 50 ~ 70 DEG C by Fructus Crataegi, then pulverizes, and crosses 60
~ 100 mesh sieves, standby.
Preferably, described Ampelopsis grossedentata extrat prepares in the following way: take Ampelopsis grossedentata with solid-liquid ratio 1:(25 ~ 35) add distillation
Water, after extracting 20 ~ 40 min under 80 ~ 100 DEG C of waters bath with thermostatic control, microwave heating 60 ~ 120s, obtain Aqueous extracts, filtered while hot, decompression
Concentrated filtrate obtains extractum, is Ampelopsis grossedentata extrat, standby.
Preferably, described propolis extract prepares in the following way: weigh a certain amount of propolis, is 1 according to solid-liquid ratio:
(25 ~ 30) add the ethanol water that volume fraction is 60 ~ 80 %, carry out supersound extraction, extract 15 ~ 20 min at 20 ~ 25 DEG C,
Then being centrifuged 5 min with 3000 r/min, pour out supernatant, residue repeats supersound extraction 3 ~ 4 times according to the method described above, in merging
Clear liquid, after 4 DEG C of cold preservation 12 h, is centrifuged 5 min with 3600 r/min, takes supernatant concentrating under reduced pressure and obtains extractum, is propolis and carries
Take thing, standby.Preferably, the frequency of described supersound extraction is 60 kHz.
The present invention also provides for, in the preparation method of a kind of prescription that relieves the effect of alcohol, comprising the steps:
S1. the preparation of Semen Hoveniae (Fructus Hoveniae) extract: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, crosses 40 mesh sieves；According to solid-liquid ratio 1:(5 ~ 7) volume integral
Number is the ethanol solution of 70 ~ 90 %, in 70 ~ 90 DEG C of thermostat water baths, reflux, extract, 1 ~ 3 h, extracts 2 ~ 3 times；Filter, merge
Filtrate, is evaporated to without alcohol taste；With the ethanol solution precipitate with ethanol that volume fraction is 95 % of 3 times of volumes, stand overnight in 4 DEG C；
Repetitive operation 2 times, merges twice molten part of alcohol, and concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, uses acetic acid second
Ester extracts, concentrating under reduced pressure extraction solution, and vacuum drying obtains acetic acid ethyl ester extract extractum, is Semen Hoveniae (Fructus Hoveniae) extract, standby
With；
S2. the preparation of Radix Puerariae extract: by Radix puerariae root raw material pulverizing, crosses 40 mesh sieves；It is first according to solid-liquid ratio 1:(10 ~ 15) add
Enter the disodium hydrogen phosphate-citrate buffer solution of pH 3.6 ~ 4.5, be subsequently adding the cellulase that mass concentration is 0.3 ~ 0.5 %,
After extracting 50 ~ 70 min at 40 ~ 50 DEG C, being filtered by extracting solution, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract, standby；
S3. the preparation of Fructus Crataegi powder: cleaned by dry Fructus Crataegi slice, dries at 50 ~ 70 DEG C, then pulverizes, and crosses 60 ~ 100 mesh sieves, standby
With；
S4. the preparation of Ampelopsis grossedentata extrat: weigh a certain amount of Ampelopsis grossedentata, with solid-liquid ratio 1:(25 ~ 35) add distilled water, 80 ~
After extracting 20 ~ 40 min under 100 DEG C of waters bath with thermostatic control, then microwave heating 60 ~ 120 s, obtain Aqueous extracts, filtered while hot, concentrating under reduced pressure
Filtrate obtains extractum, is Ampelopsis grossedentata extrat, standby；
S5. the preparation of propolis extract: weigh a certain amount of propolis, is 1:(25 ~ 30 according to solid-liquid ratio) add volume fraction be 60
The ethanol water of ~ 80 %, carries out supersound extraction, and supersonic frequency is 60 kHz, control ultrasonic temperature 20 ~ 25 DEG C extract 15 ~
20 min, are then centrifuged 5 min with 3000 r/min, pour out supernatant, and residue repeats supersound extraction 3 ~ 4 according to the method described above
Secondary, merge supernatant, after 4 DEG C of cold preservation 12 h, be centrifuged 5 min with 3600 r/min, take supernatant concentrating under reduced pressure and obtain extractum, i.e.
For propolis extract, standby；
S6. mix according to the mass fraction proportioning of each component of described prescription, adjuvant of arranging in pairs or groups, prepare described in relieve the effect of alcohol prescription.
Preferably, described S6 comprises the steps:
S61. Fructus Crataegi powder and Semen Hoveniae (Fructus Hoveniae) extract, Radix Puerariae extract, Ampelopsis grossedentata extrat, propolis extract mix homogeneously, 50 DEG C of bakings are taken
Dry, crushed after being dried becomes fine powder stand-by；
S62. the fine powder obtained is mixed homogeneously with customary adjuvant, be prepared as capsule, tablet, pill or granule, close after testing
After lattice, aseptic loading vacuum packaging bag seals, prepare described in relieve the effect of alcohol the product of prescription.
Compared to prior art, there is advantages that this prescription is by reasonable compatibility, integrated use is clear
The methods such as thermal detoxification, promoting the circulation of QI to induce diuresis, reparation hepatic injury, regulate function, and because of the propolis higher containing nutritious valency, because of
This also has the advantages such as tonic, dispelling effects of alcohol be good, can effectively alleviate alcohol intolerance, the after drinking discomfort such as headache, gastric acid, with
And repair the hepatic injury that insobriety causes.The prescription that relieves the effect of alcohol of the present invention can be prepared as capsule or tablet, is convenient for carrying, and without appointing
What toxic and side effects.
Detailed description of the invention
Below in conjunction with embodiment, the technical scheme in the embodiment of the present invention is clearly and completely described.
Hovenia Rhamnaceae Hovenia plant, has another name called chicken feet Lee, papaw, trifoliate orange etc., and edible part is fruit stem (inflorescence
Axle) and non-fruit.China eats medicinal Semen Hoveniae (Fructus Hoveniae) existing more than 1500 year, describes in Compendium of Material Medica: trifoliate orange woody flavour is sweet, property flat, nontoxic,
There is relieving restlessness of quenching the thirst, remove heat on diaphragm, moistening five ZANG-organs, profit defecation, the same Mel of merit.Semen Hoveniae (Fructus Hoveniae) contains flavonoid, polysaccharide, saponins, has
Machine acid, multivitamin and mineral isoreactivity material, have promotion urine drains, accelerates the effects such as intestinal peristalsis promoting,
Can expand the blood volume of human body, again can alcoholic intoxication, therefore can smoothening secretion, relieving alcoholic intoxication is calmed the nerves.Toxicological experiment shows that Semen Hoveniae (Fructus Hoveniae) is to people
Body has no side effect, and is a kind of natural green food, has huge DEVELOPMENT PROSPECT.
Radix Puerariae is legume pueraria lobata or the dry root of Radix Puerariae rattan, becomes containing osajin, triterpenes, aromatics isoreactivity
Point, wherein isoflavonoid puerarin, daiazi and daidzein are the main chemical compositions of Radix Puerariae, can promote blood
In alcohol dehydrogenase enzyme concentration rise, also contribute to the digestive tract absorption to ethanol simultaneously, thus it be dense to reduce the ethanol in blood
Degree, reaches preventing drunkenness, controls drunk effect.
Fructus Crataegi, has another name called fruit, Fructus Pyracanthae in mountain, and for the fruit of rosaceous plant Fructus Crataegi, matter is hard, and sarcocarp is thin, and taste is micro-sour and astringent, ripe
Fruit can give birth to or parching to brown Jiao with being used as medicine.Research shows, rich in saccharide, protein, fat, vitamin C, carotene in Fructus Crataegi
And the material such as calcium and ferrum, it is possible to spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, expand blood vessel, reduce blood pressure, it is possible to improve and
Promote that Cholesterol Excretion reduces blood fat, the generation of prevention hyperlipidemia.Flavonoid contained by Fructus Crataegi and vitamin C, carotene
The generation of free radical can also be blocked and reduce Deng material, can the immunity of enhancing body, slow down aging, cancer-resisting.
Ampelopsis grossedentata is the young stem and leaf of a kind of Wild Liane in vitaceae Ampelopsis, is the distinctive medicated tea of China
Dual-purpose plant, is grown on the high mountain region of height above sea level 200 ~ 800 m more, be distributed mainly on Guangxi, Guangdong, Yunnan, Guizhou, Hunan,
The ground such as Hubei, Jiangxi, Fujian.Its stem and leaf is usually used in treating the diseases such as cold, fever, laryngopharynx swelling and pain, icterohepatitis, existing hundreds of
The use history in year.The chemical composition of Ampelopsis grossedentata is mainly flavonoid, oligomeric stilbene class, phenols, has antiinflammatory, antibacterial, antioxidation, guarantor
The multiple efficacies such as protecting liver, blood fat reducing, blood sugar lowering, enhancing immunity and suppression tumor.The wherein dihydromyricetin in flavonoid
Rich content, in spire stem, content accounts for 20 more than % of dry weight, and up to 40 % in spire, research shows, dihydromyricetin
There is the effect of hepatoprotective and anti-hepatic fibrosis.
Propolis is the natural gum that Apis gathers at plant sprout bud and trunk, and is mixed into Apis mandibular gland secretions and Cera Flava etc.
A kind of colloidal solid material with special aromatic odor processed.Propolis contains alkyl acid oil, oils and fats, aminoacid, has
Machine acid, ferrum, copper, manganese, zinc, phytocide, vitamin B group, vitamin E, vitamin C, biotin, provitamin A, anti-
The material such as rhzomorph, enzyme, possibly together with multiple natural effective active matter, such as flavonoid, phenolic acid etc., has regulation blood glucose, antibacterial, anti-
The multiple pharmacologically actives such as viral, anticancer, blood fat reducing, enhancing immunity.Above-mentioned abundant active component synergism, makes propolis pair
Ethanol causes that hepatic injury is protected and repair.Therefore, cause liver prejudicial for long-term alcohol or have Chronic Liver
The people that disease of ZANG-organs becomes, liver can be played certain protective effect by long-term taking bee glue product.
The preparation of embodiment 1 Semen Hoveniae (Fructus Hoveniae) extract
Method I: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, crosses 40 mesh sieves；According to the ethanol solution that volume fraction is 70 % of solid-liquid ratio 1:5,
In 70 DEG C of thermostat water baths, reflux, extract, 1 h, extracts 2 times；Filter, merging filtrate, be evaporated to without alcohol taste；With 3 times
The volume fraction of volume is the ethanol solution precipitate with ethanol of 95 %, stands overnight in 4 DEG C of refrigerators；Repetitive operation 2 times, merges twice
The molten part of alcohol, concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, extracts by ethyl acetate, concentrating under reduced pressure
Extraction solution, vacuum drying, obtain acetic acid ethyl ester extract extractum, be Semen Hoveniae (Fructus Hoveniae) extract.
Method II: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, crosses 40 mesh sieves；The ethanol that volume fraction is 80 % according to solid-liquid ratio 1:6
Solution, in 80 DEG C of thermostat water baths, reflux, extract, 2 h, extracts 3 times.Filter, merging filtrate, be evaporated to without alcohol taste；
With the ethanol solution precipitate with ethanol that volume fraction is 95 % of 3 times of volumes, stand overnight in 4 DEG C of refrigerators；Repetitive operation 2 times, merges
Twice molten part of alcohol, concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, extracts by ethyl acetate, decompression
Concentrate extraction solution, vacuum drying, obtain acetic acid ethyl ester extract extractum, be Semen Hoveniae (Fructus Hoveniae) extract.
Method III: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, crosses 40 mesh sieves.The ethanol that volume fraction is 90 % according to solid-liquid ratio 1:7
Solution, in 90 DEG C of thermostat water baths, reflux, extract, 3 h, extracts 3 times.Filter, merging filtrate, be evaporated to without alcohol taste；
With the ethanol solution precipitate with ethanol that volume fraction is 95 % of 3 times of volumes, stand overnight in 4 DEG C of refrigerators；Repetitive operation 2 times, merges
Twice molten part of alcohol, concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, extracts by ethyl acetate, decompression
Concentrate extraction solution, vacuum drying, obtain acetic acid ethyl ester extract extractum, be Semen Hoveniae (Fructus Hoveniae) extract.
Utilize above-mentioned three kinds of methods all can obtain Semen Hoveniae (Fructus Hoveniae) extract of the present invention, it is highly preferred that method II turns
The extraction efficiency of Fructus Jujubae extract is best, and yield is the highest, for giving full play to the sober-up function of Semen Hoveniae (Fructus Hoveniae), improves its utilization rate, selected side
The Semen Hoveniae (Fructus Hoveniae) extract obtained in method II relieves the effect of alcohol for embodiment 6 preparation of prescription.
The preparation of embodiment 2 Radix Puerariae extract
Method I: by Radix puerariae root raw material pulverizing, crosses 40 mesh sieves；It is first according to the phosphoric acid hydrogen two that solid-liquid ratio 1:10 adds pH 3.6
Sodium-citrate buffer solution, is subsequently adding the cellulase that mass concentration is 0.3 %, after extracting 50 min, will carry at 40 DEG C
Taking liquid to filter, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract.
Method II: by Radix puerariae root raw material pulverizing, crosses 40 mesh sieves；It is first according to the phosphoric acid that solid-liquid ratio 1:12 adds pH 4.0
Disodium hydrogen-citrate buffer solution, is subsequently adding the cellulase that mass concentration is 0.4 %, after extracting 60 min at 45 DEG C,
Being filtered by extracting solution, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract.
Method III: by Radix puerariae root raw material pulverizing, crosses 40 mesh sieves；It is first according to the phosphoric acid that solid-liquid ratio 1:15 adds pH 4.5
Disodium hydrogen-citrate buffer solution, is subsequently adding the cellulase that mass concentration is 0.5 %, after extracting 70 min at 50 DEG C,
Being filtered by extracting solution, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract.
Utilize above-mentioned three kinds of methods all can obtain Radix Puerariae extract of the present invention, it is highly preferred that Pueraria lobota in method II
The extraction ratio of root is the highest, and Extraction solvent volume is moderate, and energy consumption is less, and in the extract obtained, flavonoid is with puerarin content more
Height, considers extraction ratio and after the time, and the Radix Puerariae extract obtained in method for selecting II relieves the effect of alcohol the joining of prescription for embodiment 6
System.
The preparation of embodiment 3 Fructus Crataegi powder
Method I: cleaned by dry Fructus Crataegi slice, dries at 50 DEG C, then pulverizes, and crosses 60 mesh sieves.
Method II: cleaned by dry Fructus Crataegi slice, dries at 60 DEG C, then pulverizes, and crosses 100 mesh sieves.
Method III: cleaned by dry Fructus Crataegi slice, dries at 70 DEG C, then pulverizes, and crosses 80 mesh sieves.
Utilize above-mentioned three kinds of methods all can obtain Fructus Crataegi powder of the present invention, consider drying temperature in Fructus Crataegi
The impact of active component, and degree of grinding, for improving its utilization rate, it is highly preferred that the drying temperature in method II and particle diameter
More suitable.Therefore, the Fructus Crataegi powder obtained in method for selecting II is relieved the effect of alcohol for embodiment 6 preparation of prescription.
The preparation of embodiment 4 Ampelopsis grossedentata extrat
Method I: weigh a certain amount of Ampelopsis grossedentata, adds distilled water with solid-liquid ratio 1:25, extracts 20 min under 80 DEG C of waters bath with thermostatic control
After, placing in microwave oven, under low grade, heat 60s, obtain Aqueous extracts, filtered while hot, concentrating under reduced pressure filtrate obtains extractum, is rattan
Tea extract.
Method II: weigh a certain amount of Ampelopsis grossedentata, adds distilled water with solid-liquid ratio 1:30, extracts under 100 DEG C of waters bath with thermostatic control
After 30 min, placing in microwave oven, at medium-to-high grade lower heating 90s, obtain Aqueous extracts, filtered while hot, concentrating under reduced pressure filtrate must be soaked
Cream, is Ampelopsis grossedentata extrat.
Method III: weigh a certain amount of Ampelopsis grossedentata, adds distilled water with solid-liquid ratio 1:35, extracts 40 under 90 DEG C of waters bath with thermostatic control
After min, placing in microwave oven, heat 120s, obtain Aqueous extracts, filtered while hot under top grade, concentrating under reduced pressure filtrate obtains extractum, i.e.
For Ampelopsis grossedentata extrat.
Utilizing above-mentioned three kinds of methods all can obtain Ampelopsis grossedentata extrat of the present invention, three kinds of extracting modes are compared, more
Preferably, the Ampelopsis grossedentata extrat content obtained in method II is the highest, and energy consumption is less, considers extraction ratio, Extracting temperature, carries
Take that liquid is long-pending, after energy consumption, the Ampelopsis grossedentata extrat obtained in method for selecting II relieves the effect of alcohol for embodiment 6 preparation of prescription.
The preparation of embodiment 5 propolis extract
Method I: weigh a certain amount of propolis, is the ethanol water that 1:25 adds that volume fraction is 60 % according to solid-liquid ratio, carries out
Supersound extraction, supersonic frequency is 60 kHz, controls ultrasonic temperature and extracts 15 min at 20 DEG C, is then centrifuged 5 with 3000 r/min
Min, pours out supernatant, and residue repeats supersound extraction 3 times according to the method described above, merges supernatant, in 4 DEG C of refrigerator cold-storage 12 h
After, it is centrifuged 5 min with 3600 r/min, takes supernatant concentrating under reduced pressure and obtain extractum, be propolis extract.
Method II: weigh a certain amount of propolis, is the ethanol water that 1:27 adds that volume fraction is 70 % according to solid-liquid ratio,
Carrying out supersound extraction, supersonic frequency is 60 kHz, control ultrasonic temperature 23 DEG C extract 17 min, then with 3000 r/min from
The heart 5 min, pours out supernatant, and residue repeats supersound extraction 4 times according to the method described above, merges supernatant, in 4 DEG C of refrigerator cold-storages
After 12 h, it is centrifuged 5 min with 3600 r/min, takes supernatant concentrating under reduced pressure and obtain extractum, be propolis extract.
Method III: weigh a certain amount of propolis, is the ethanol water that 1:30 adds that volume fraction is 80 % according to solid-liquid ratio,
Carrying out supersound extraction, supersonic frequency is 60 kHz, control ultrasonic temperature 25 DEG C extract 20 min, then with 3000 r/min from
The heart 5 min, pours out supernatant, and residue repeats supersound extraction 4 times according to the method described above, merges supernatant, in 4 DEG C of refrigerator cold-storages
After 12 h, it is centrifuged 5 min with 3600 r/min, takes supernatant concentrating under reduced pressure and obtain extractum, be propolis extract.
Utilize above-mentioned three kinds of methods all can obtain propolis extract of the present invention, it is highly preferred that honeybee in method III
The extraction efficiency of glue is the highest, and utilization rate is best, and the propolis extract obtained in method for selecting III relieves the effect of alcohol prescription in embodiment 6
Preparation.
Embodiment 6 prepares the prescription that relieves the effect of alcohol by weight
Optimal preparation method selected in embodiment 1 ~ 5 is used to prepare described Semen Hoveniae (Fructus Hoveniae) extract, Radix Puerariae extract, Fructus Crataegi
Powder, Ampelopsis grossedentata extrat and propolis extract, relieve the effect of alcohol prescription according to the preparation of following parts by weight:
Formula is 1.: Semen Hoveniae (Fructus Hoveniae) extract 2 parts, Radix Puerariae extract 8 parts, Fructus Crataegi powder 50 parts, Ampelopsis grossedentata extrat 15 parts, propolis extract 15
Part.
Formula is 2.: Semen Hoveniae (Fructus Hoveniae) extract 10 parts, Radix Puerariae extract 1 part, Fructus Crataegi powder 30 parts, Ampelopsis grossedentata extrat 5 parts, and propolis extracts
Thing 5 parts.
Formula is 3.: Semen Hoveniae (Fructus Hoveniae) extract 5 parts, Radix Puerariae extract 4 parts, Fructus Crataegi powder 40 parts, Ampelopsis grossedentata extrat 10 parts, and propolis extracts
Thing 10 parts.
Control formula A: basically identical with formula 3. middle component and ratio, difference is described Ampelopsis grossedentata extrat and honeybee
Glue extract is 1 part.
Control formula B: basically identical with formula 3. middle component and ratio, difference is described Ampelopsis grossedentata extrat and honeybee
Glue extract is 20 parts.
Control formula C: Semen Hoveniae (Fructus Hoveniae) extract 2 parts, Radix Puerariae extract 1 part, Fructus Crataegi powder 40 parts, Ampelopsis grossedentata extrat 20 parts, propolis
Extract 20 parts.
By above-mentioned formula 1. ~ 3. and control formula A ~ C is prepared as the prescription that relieves the effect of alcohol the most according to the methods below:
S61. Fructus Crataegi powder and Semen Hoveniae (Fructus Hoveniae) extract, Radix Puerariae extract, Ampelopsis grossedentata extrat, propolis extract mix homogeneously, 50 DEG C of bakings are taken
Dry, crushed after being dried becomes fine powder stand-by；
S62. the fine powder obtained is mixed homogeneously with adjuvant, be prepared as capsule, tablet, pill or granule, the most qualified
After, aseptic loading vacuum packaging bag seals, prepare described in relieve the effect of alcohol the product of prescription.
Above-mentioned formula is carried out mouse experiment, formula 1. ~ 3. in, it is preferable that with formula 3. as representative, arrange basic, normal, high
3 dosage groups, and with the high dose group of control formula A ~ C as a comparison, investigate the effect of its relieving alcoholism and protecting the liver.
1, relieve the effect of alcohol prescription preventing drunkenness, relieving alcoholic intoxication experiment
Choose SPF level Male Kunming strain mice 105, be randomly divided into 7 groups, often group 15, respectively model group, formula the lowest,
Middle and high 3 dosage groups, control formula A high dose group, control formula B high dose group and control formula C high dose group.Each group is little
Mus, with fed standard chow, freely drinks water, and laboratory adaptability is tested after raising 1 week.According to mice and body surface area
Equivalent Conversion ratio calculation mice consumption, by respectively relieving the effect of alcohol, prescription fine powder normal saline is prepared as suspension.Before experiment, each group is dynamic
Thing fasting 8 h, model group gives normal saline, formula basic, normal, high dosage treated animal relieve the effect of alcohol respectively prescription 0.28,
0.56 and 1.12 g/ (kg d), control formula A high dose group, control formula B high dose group and control formula C high dose component
Not giving 1.12 g/ (kg d), after gavage 45 min, each treated animal gives 56 ° of Red Star strong, colourless liquor distilled from sorghum Chinese liquor (15 mL/kg).See
Examine mice from filling wine to drunk, then the situation to relieving alcoholic intoxication.Whether mice is the most drunk, disappear as index with righting reflex, keeps
Back downward posture 30 more than s person thinks drunk；Whether whether relieving alcoholic intoxication reverts to index with righting reflex to mice.Record drunk
Number of animals, mice sleeping duration (drunk time) and recovery time (relieving alcoholic intoxication time), the results are shown in Table 1.
Data from table 1: it is drunk that formula 3. low dose group has 9 animals to occur, middle and high dosage component do not have 5,
2 animals are drunk, control formula A high dose group, control formula B high dose group and control formula C high dose group have respectively 3,2
Only, 8 animals drunk, and model group has 14 animals drunk.Compare with model group, before wine, fill the prescription that relieves the effect of alcohol, the most each dose of formula
Amount group, control formula A high dose group and control formula B high dose group all can be obviously prolonged mice sleeping duration, and
Shortening mice righting reflex recovery time (P < 0.05), each group data are statistically significant；The effect of control formula C high dose group
The most notable.Analyze its reason, be because Semen Hoveniae (Fructus Hoveniae) extract in control formula C high dose group too low with the ratio of Radix Puerariae extract,
Unreasonable with other component blend proportions, it is seen that in the prescription that relieves the effect of alcohol of the present invention, Semen Hoveniae (Fructus Hoveniae) extract and Radix Puerariae extract are as master
The importance of composition to be relieved the effect of alcohol.
2, relieve the effect of alcohol the prescription protective effect to acute liver damage
Choose SPF level Male Kunming strain mice 120, be randomly divided into 8 groups, often group 15, be respectively blank group, model group, join
Basic, normal, high 3 the dosage groups in side, control formula A high dose group, control formula B high dose group and control formula C high dose group.
Each group mice, with fed standard chow, freely drinks water, and adaptability enters experiment after feeding 1 week.Each group mice fasting 8 before experiment
H, blank group, model group give normal saline, and formula basic, normal, high dosage treated animal relieves the effect of alcohol prescription 0.28,0.56 respectively
With 1.12 g/ (kg d), control formula A high dose group, control formula B high dose group are given respectively with control formula C high dose group
Give 1.12 g/ (kg d), 1 time/d, continuous 30 d.After last gavage 45 min, in addition to blank group gives normal saline, its
He respectively organizes mice and all gives 56 ° of Red Star strong, colourless liquor distilled from sorghum Chinese liquor (15 mL/kg).Water 12 h posterior orbit venous blood sampling is can't help in fasting, and 3500
R/min, low-temperature centrifugation 10 min, separate serum, and-20 DEG C of Refrigerator stores, according in test kit illustration method detection mice serum
Alanine aminotransferase (ALT), aspartate aminotransferase (AST), the activity of ethanol dehydrogenase (ADH), the results are shown in Table
2.Mice is put to death in dislocation, takes liver, weighs the hepatic tissue blocking of 0.5 g, prepares 10% liver homogenate with ice normal saline, measures liver
Middle superoxide dismutase (SOD) and reduced glutathion (GSH) activity, triglyceride (TG) and malonaldehyde (MDA) content,
The results are shown in Table 3.
From the data of table 2, with blank group ratio, acute alcohol liver injury model group Serum ALT, AST, ADH contain
Measure the most significantly raised (P < 0.05), Models of Acute Alcoholic Liver Injury success is described；After prescription intervention of relieving the effect of alcohol processes, formula is the most each
ALT, AST, ADH level relatively model group of dosage group serum the most substantially reduces (P < 0.05), wherein high dose group Serum ALT,
AST, ADH level compared with in, low dose group low (P < 0.05).With formula 3. high dose group ratio, control formula A high dose group reduces blood
Clear ALT, AST, ADH level is inconspicuous；And control formula B high dose group and control formula C high dose group to Serum ALT, AST,
The reduction level relatively formula 3. high dose group of ADH does not has significant difference.
From the data of table 3, TG, MDA content of model group is significantly higher than blank group (P < 0.05), SOD, GSH content
It is substantially less than blank group (P < 0.05), illustrates that liver is caused damage by ethanol.Formula the most each dosage group all can reduce liver TG,
MDA level, increased SOD, GSH content (P < 0.05), wherein TG, MDA level of formula 3. high dose group compared with in, low dose group low
(P < 0.05), SOD, GSH content of formula 3. high dose group relatively in, low dose group high (P < 0.05)；With formula 3. high dose group
Ratio, control formula A high dose group reduces TG, MDA level, increased SOD, GSH content DeGrain；Control formula B high dose
Group and control formula C high dose group are to reducing TG, MDA level, increased SOD, GSH content relatively formula 3. high dose group the most substantially
Difference.
In summary: the prescription that relieves the effect of alcohol of the present invention relieves the effect of alcohol, hepatoprotective effect preferable, compare between formula basic, normal, high dosage group
Relatively, the most notable with high dose relieving alcoholism and protecting the liver sheet effect.Ampelopsis grossedentata extrat and propolis extract in control formula A high dose group
Parts by weight are relatively low, and its ability relatively formula that relieves the effect of alcohol is 3. without significant difference, but hepatoprotective ability is more weak.Control formula B high dose group
The parts by weight of middle Ampelopsis grossedentata extrat and propolis extract are beyond the highest scope of the present invention, it is seen that along with Ampelopsis grossedentata extrat and honeybee
The increase of glue extract ratio, its hepatoprotective ability is also not remarkably increased, and cost of material increases.Control formula C high dose group
Middle Semen Hoveniae (Fructus Hoveniae) extract is relatively low with the ratio of Radix Puerariae extract, and the ratio of Ampelopsis grossedentata extrat and propolis extract is higher than the present invention's
Restriction scope, so this formula dispelling effects of alcohol is poor, and hepatoprotective ability and control formula C high dose group no significant difference.Therefore,
This result show the gavage present invention relieve the effect of alcohol prescription for mice liver resist ethanol lipid peroxidation injury have the most auxiliary
Help effect；And relieve the effect of alcohol prescription ratio described in changing, appreciable impact prescription solution is waken up and quenches the thirst, arrange alcoholism and the effect of reparation hepatic injury
Really, or increase the preparation cost of prescription of relieving the effect of alcohol.
Obviously, embodiment described above is only a part of embodiment of the present invention rather than whole embodiments, other
The conventional tablet that relieves the effect of alcohol, pill or granulate excipients component, does not the most destroy the active component of product, does not affect active component
The performance of effect, broadly falls into protection scope of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art are not having
There is on the premise of making creative work the every other embodiment obtained, broadly fall into the scope of protection of the invention.
Claims (8)
1. the prescription that relieves the effect of alcohol, it is characterised in that include each component of following parts by weight: Semen Hoveniae (Fructus Hoveniae) extract 2 ~ 10 parts, Radix Puerariae
Extract 1 ~ 8 part, Fructus Crataegi powder 30 ~ 50 parts, Ampelopsis grossedentata extrat 5 ~ 15 parts, propolis extract 5 ~ 15 parts.
The prescription that relieves the effect of alcohol the most according to claim 1, it is characterised in that described Semen Hoveniae (Fructus Hoveniae) extract prepares in the following way:
By dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, by solid-liquid ratio 1:(5 ~ 7) add the ethanol solution that volume fraction is 70 ~ 90 %, in 70 ~ 90 DEG C next time
Stream extraction 1 ~ 3 h, extracts 2 ~ 3 times；Filter, merging filtrate, be evaporated to without alcohol taste；Molten with the ethanol that volume fraction is 95%
Liquid precipitate with ethanol, stands overnight in 4 DEG C；Repetitive operation, merges the molten part of alcohol, and concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added appropriate
Water suspendible, extracts by ethyl acetate, concentrating under reduced pressure extraction solution, and vacuum drying obtains acetic acid ethyl ester extract extractum, is
Semen Hoveniae (Fructus Hoveniae) extract, standby.
The prescription that relieves the effect of alcohol the most according to claim 1, it is characterised in that described Radix Puerariae extract prepares in the following way:
By Radix puerariae root raw material pulverizing, according to solid-liquid ratio 1:(10 ~ 15) add the disodium hydrogen phosphate-citric acid buffering of pH 3.6 ~ 4.5
Liquid, is subsequently adding the cellulase that mass concentration is 0.3 ~ 0.5 %, after extracting 50 ~ 70 min at 40 ~ 50 DEG C, by extracting solution
Filtering, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract, standby.
The prescription that relieves the effect of alcohol the most according to claim 1, it is characterised in that described Fructus Crataegi powder prepares in the following way: by mountain
Short, bristly hair or beard is dried at 50 ~ 70 DEG C, then pulverizes, and crosses 60 ~ 100 mesh sieves, standby.
The prescription that relieves the effect of alcohol the most according to claim 1, it is characterised in that described Ampelopsis grossedentata extrat prepares in the following way:
Take Ampelopsis grossedentata with solid-liquid ratio 1:(25 ~ 35) add distilled water, after extracting 20 ~ 40 min under 80 ~ 100 DEG C of waters bath with thermostatic control, microwave adds
Heat 60 ~ 120s, obtains Aqueous extracts, filtered while hot, and concentrating under reduced pressure filtrate obtains extractum, is Ampelopsis grossedentata extrat, standby.
The prescription that relieves the effect of alcohol the most according to claim 1, it is characterised in that described propolis extract prepares in the following way:
Weigh a certain amount of propolis, be 1:(25 ~ 30 according to solid-liquid ratio) add the ethanol water that volume fraction is 60 ~ 80 %, surpass
Sound extracts, and extracts 15 ~ 20 min at 20 ~ 25 DEG C, is then centrifuged 5 min with 3000 r/min, pours out supernatant, and residue is according to upper
Method of stating repeats supersound extraction 3 ~ 4 times, merges supernatant, after 4 DEG C of cold preservation 12 h, is centrifuged 5 min with 3600 r/min, takes
Clear liquid concentrating under reduced pressure obtains extractum, is propolis extract, standby.
7. relieve the effect of alcohol described in claim 1 preparation method of prescription, it is characterised in that comprise the steps:
S1. the preparation of Semen Hoveniae (Fructus Hoveniae) extract: by dry Semen Hoveniae (Fructus Hoveniae) raw material pulverizing, crosses 40 mesh sieves；According to solid-liquid ratio 1:(5 ~ 7) volume integral
Number is the ethanol solution of 70 ~ 90 %, in 70 ~ 90 DEG C of thermostat water baths, reflux, extract, 1 ~ 3 h, extracts 2 ~ 3 times；Filter, close
And filtrate, it is evaporated to without alcohol taste；With the ethanol solution precipitate with ethanol that volume fraction is 95 % of 3 times of volumes, stood in 4 DEG C
Night；Repetitive operation 2 times, merges twice molten part of alcohol, and concentrating under reduced pressure obtains extractum；Above-mentioned extractum is added suitable quantity of water suspendible, uses second
Acetoacetic ester extracts, concentrating under reduced pressure extraction solution, and vacuum drying obtains acetic acid ethyl ester extract extractum, is Semen Hoveniae (Fructus Hoveniae) extract,
Standby；
S2. the preparation of Radix Puerariae extract: by Radix puerariae root raw material pulverizing, crosses 40 mesh sieves；It is first according to solid-liquid ratio 1:(10 ~ 15) add
Enter the disodium hydrogen phosphate-citrate buffer solution of pH 3.6 ~ 4.5, be subsequently adding the cellulase that mass concentration is 0.3 ~ 0.5 %,
After extracting 50 ~ 70 min at 40 ~ 50 DEG C, being filtered by extracting solution, concentrating under reduced pressure obtains extractum, is Radix Puerariae extract, standby；
S3. the preparation of Fructus Crataegi powder: cleaned by dry Fructus Crataegi slice, dries at 50 ~ 70 DEG C, then pulverizes, and crosses 60 ~ 100 mesh sieves, standby
With；
S4. the preparation of Ampelopsis grossedentata extrat: weigh a certain amount of Ampelopsis grossedentata, with solid-liquid ratio 1:(25 ~ 35) add distilled water, 80 ~
After extracting 20 ~ 40 min under 100 DEG C of waters bath with thermostatic control, then microwave heating 60 ~ 120 s, obtain Aqueous extracts, filtered while hot, concentrating under reduced pressure
Filtrate obtains extractum, is Ampelopsis grossedentata extrat, standby；
S5. the preparation of propolis extract: weigh a certain amount of propolis, is 1:(25 ~ 30 according to solid-liquid ratio) add volume fraction be
The ethanol water of 60 ~ 80 %, carries out supersound extraction, and supersonic frequency is 60 kHz, controls ultrasonic temperature 20 ~ 25 DEG C of extractions
15 ~ 20 min, are then centrifuged 5 min with 3000 r/min, pour out supernatant, residue repeat according to the method described above supersound extraction 3 ~
4 times, merge supernatant, after 4 DEG C of cold preservation 12 h, be centrifuged 5 min with 3600 r/min, take supernatant concentrating under reduced pressure and obtain extractum,
It is propolis extract, standby；
S6. mix according to the mass fraction proportioning of each component of described prescription, adjuvant of arranging in pairs or groups, prepare described in relieve the effect of alcohol prescription.
The preparation method of the prescription that relieves the effect of alcohol the most according to claim 7, it is characterised in that described S6 comprises the steps:
S61. Fructus Crataegi powder and Semen Hoveniae (Fructus Hoveniae) extract, Radix Puerariae extract, Ampelopsis grossedentata extrat, propolis extract mix homogeneously, 50 DEG C of bakings are taken
Dry, crushed after being dried becomes fine powder stand-by；
S62. the fine powder obtained is mixed homogeneously with customary adjuvant, be prepared as capsule, tablet, pill or granule, close after testing
After lattice, aseptic loading vacuum packaging bag seals, prepare described in relieve the effect of alcohol the product of prescription.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107348059A (en) * | 2017-09-01 | 2017-11-17 | 黄山华绿园生物科技有限公司 | Antialcoholic tea and its manufacture craft |
| CN109674896A (en) * | 2019-01-23 | 2019-04-26 | 武汉藻优生物科技有限公司 | A kind of vine tea flavone extract and its preparation method and application |
| CN110897066A (en) * | 2019-11-04 | 2020-03-24 | 广州赢联健康产业有限公司 | Semen hoveniae sobering-up beverage |
| CN112717035A (en) * | 2021-02-03 | 2021-04-30 | 沃久(杭州)生物医药科技有限公司 | Traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and preparation method and application thereof |
| CN114557411A (en) * | 2022-03-15 | 2022-05-31 | 山东省农业科学院 | Liver-protecting and alcoholism-relieving compound beverage and preparation method thereof |
| CN115211557A (en) * | 2022-07-23 | 2022-10-21 | 尚食(无锡)食品科技开发有限公司 | Hangover-alleviating and liver-protecting composition and preparation method of tablet containing same |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107348059A (en) * | 2017-09-01 | 2017-11-17 | 黄山华绿园生物科技有限公司 | Antialcoholic tea and its manufacture craft |
| CN109674896A (en) * | 2019-01-23 | 2019-04-26 | 武汉藻优生物科技有限公司 | A kind of vine tea flavone extract and its preparation method and application |
| CN110897066A (en) * | 2019-11-04 | 2020-03-24 | 广州赢联健康产业有限公司 | Semen hoveniae sobering-up beverage |
| CN112717035A (en) * | 2021-02-03 | 2021-04-30 | 沃久(杭州)生物医药科技有限公司 | Traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and preparation method and application thereof |
| CN114557411A (en) * | 2022-03-15 | 2022-05-31 | 山东省农业科学院 | Liver-protecting and alcoholism-relieving compound beverage and preparation method thereof |
| WO2023173947A1 (en) * | 2022-03-15 | 2023-09-21 | 山东省农业科学院 | Liver-protecting and hangover-alleviating composite beverage and preparation method therefor |
| CN115211557A (en) * | 2022-07-23 | 2022-10-21 | 尚食(无锡)食品科技开发有限公司 | Hangover-alleviating and liver-protecting composition and preparation method of tablet containing same |
| CN115381014A (en) * | 2022-07-29 | 2022-11-25 | 湖南食品药品职业学院 | Hangover-alleviating and liver-protecting functional solid beverage |
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