CN105853515A - Traditional Chinese medicine composition for treating acne and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating acne and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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Abstract
The invention discloses a traditional Chinese medicine composition for treating acne. The traditional Chinese medicine composition is composed of Chinese rhubarb extract and dandelion extract and can be made into any pharmaceutically acceptable oral dosage form, including a tablet, a capsule, a granule, a pill and syrup. According to the invention, the raw materials are simple; cost is reduced compared with other compositions for treating acne; a specific traditional Chinese medicine extraction method is employed, so the recovery rates of effective components in the traditional Chinese medicines are increased; and the traditional Chinese medicine composition has improved treatment effect and small toxic and side effect.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition treating acne and preparation method thereof, belong to doctor
Medicine field.
Background technology
Acne is one of modal disease of department of dermatologry, is mainly in adolescence, is again comedo
, acne, folliculitis, be generally apt to occur in face, cervical region.Clinical with Whiteheads, inflammatory
Pimple, pustule, tuberosity, cyst etc. are main performance, and during outbreak, affected part is often accompanied by acute inflammation
Disease and pain reaction.Contact dermatitis (ACD) is skin or mucosa single or multiple connects
After touching exogenous material, the struvite disease that the position beyond contact site even contacts occurs
Sick.Owing to patient is difficult to thoroughly depart from corresponding contact virulence factor, therefore primary disease is the most anti-
Recurrence is made, and prolonged does not heals.
The medicine of quality acne is a lot of on the market, and dragons and fishes jumbled together, and great majority are all to take stopgap measures not control
This, the temporary transient acne having restrained surface, and also side effect is very big, also has bigger depending on
Lai Xing.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine composition treating acne and preparation side thereof
Method, is made up of extract from Radix Et Rhizoma Rhei and Herba Taraxaci respectively, and curative effect is more excellent, more stable
, and have no side effect.
A kind of Chinese medicine composition treating acne, including following traditional Chinese medicine raw: Radix Et Rhizoma Rhei, Pu Gong
English.
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 3-7 part, Herba Taraxaci extract 7-9 part.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 60-70 DEG C of the acetone adding 5-7 times of quality is heated to reflux 50-
70 minutes, filter, obtain filtrate；
(2) the 1/5-of filtrate decompression recycling design to volume
1/3, obtain concentrated solution, then use concentrated solution 2-
The petroleum ether extraction of 3 times of volumes, obtains petroleum ether part；
(3) DM-301 macroporous adsorptive resins in petroleum ether part, upper column flow rate is 1.8-
2.2BV/h, then by the methanol-eluted fractions of 3BV, elution flow rate is 1.8-
2.2BV/h, collects eluent；
(4) eluent being evaporated to relative density is 1.05-
The extractum of 1.09, is Radix Et Rhizoma Rhei extract
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 7-9 times of quality 50% ethanol 80-
90 DEG C of heating and refluxing extraction 80-120 minute, filter, obtain filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.4-
1.6BV/h, with the water of 4BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV
, elution flow rate is 1.4-1.6BV/h, collects 50% ethanol elution；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 1.8-
2.2BV/h, then with the water of 4BV, 50% ethanol of 2BV, the 70% ethanol gradient successively of 1BV is washed
De-, elution flow rate is 1.8-2.2BV/h, collects 70% ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Described peroral dosage form is: tablet, capsule, granule, pill, syrup.
Raw material of the present invention is simple, compared with the Chinese medicine composition of other treatment acne, reduces
Cost, have employed specific Traditional Chinese medicine extraction method simultaneously, has extracted the effective ingredient of acne
, improve therapeutic effect, and toxic and side effects be little.
Specific embodiment:
Embodiment 1:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 3 parts, Herba Taraxaci extract 7 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 60 DEG C of the acetone adding 5 times of quality is heated to reflux 50 minutes, mistake
Filter, obtains filtrate；
(2) the 1/5 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 2 times of volumes of liquid, obtains petroleum ether part；
(3) DM-in petroleum ether part
301 macroporous adsorptive resins, upper column flow rate is 1.8BV/h, then by the methanol-eluted fractions of 3BV, washes
Separation of flow speed is 1.8BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.05, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 80 DEG C of heating and refluxing extraction of 7 times of quality 50% ethanol 80 points
Clock, filters, obtains filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.4BV/h, with 4
The water of BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1
.4BV/h, 50% ethanol elution is collected；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 1.8BV/h, then with the water of 4BV, the 50% of 2BV
Ethanol, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 1.8BV/h, collects 70%
Ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Embodiment 2:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 5 parts, Herba Taraxaci extract 8 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 65 DEG C of the acetone adding 6 times of quality is heated to reflux 60 minutes, mistake
Filter, obtains filtrate；
(2) the 1/4 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 2.5 times of volumes of liquid, obtains petroleum ether part；
(3) DM-in petroleum ether part
301 macroporous adsorptive resins, upper column flow rate is 2.0BV/h, then by the methanol-eluted fractions of 3BV, washes
Separation of flow speed is 2.0BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.07, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 85 DEG C of heating and refluxing extraction 100 of 8 times of quality 50% ethanol
Minute, filter, obtain filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.5BV/h, with 4
The water of BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1
.5BV/h, 50% ethanol elution is collected；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 2.0BV/h, then with the water of 4BV, the 50% of 2BV
Ethanol, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 2.0BV/h, collects 70%
Ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Embodiment 3:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 7 parts, Herba Taraxaci extract 9 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 70 DEG C of the acetone adding 7 times of quality is heated to reflux 70 minutes, mistake
Filter, obtains filtrate；
(2) the 1/3 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 3 times of volumes of liquid, obtains petroleum ether part；
(3) DM-in petroleum ether part
301 macroporous adsorptive resins, upper column flow rate is 2.2BV/h, then by the methanol-eluted fractions of 3BV, washes
Separation of flow speed is 2.2BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 0.09, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 90 DEG C of heating and refluxing extraction 120 of 9 times of quality 50% ethanol
Minute, filter, obtain filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.6BV/h, with 4
The water of BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1
.6BV/h, 50% ethanol elution is collected；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 2.2BV/h, then with the water of 4BV, the 50% of 2BV
Ethanol, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 2.2BV/h, collects 70%
Ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Embodiment 4:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 10 parts, Herba Taraxaci extract 5 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 75 DEG C of the acetone adding 8 times of quality is heated to reflux 40 minutes, mistake
Filter, obtains filtrate；
(2) the 1/2 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 4 times of volumes of liquid, obtains petroleum ether part；
(3) DM-in petroleum ether part
301 macroporous adsorptive resins, upper column flow rate is 1.5BV/h, then by the methanol-eluted fractions of 3BV, washes
Separation of flow speed is 1.5BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.10, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 75 DEG C of heating and refluxing extraction 140 of 6 times of quality 50% ethanol
Minute, filter, obtain filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.8BV/h, with 4
The water of BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1
.8BV/h, 50% ethanol elution is collected；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 1.5BV/h, then with the water of 4BV, the 50% of 2BV
Ethanol, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 1.5BV/h, collects 70%
Ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Embodiment 5:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 4 parts, Herba Taraxaci extract 7 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 60 DEG C of the acetone adding 6 times of quality is heated to reflux 55 minutes, mistake
Filter, obtains filtrate；
(2) the 1/5 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 3 times of volumes of liquid, obtains petroleum ether part；
(3) D101 macroporous adsorptive resins in petroleum ether part, upper column flow rate is 1.9BV/
H, then by the methanol-eluted fractions of 3BV, elution flow rate is 1.9BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.06, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 90 DEG C of heating and refluxing extraction of 9 times of quality 50% ethanol 90 points
Clock, filters, obtains filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.4BV/h, with 4
The water of BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1
.4BV/h, 50% ethanol elution is collected；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-
201 macroporous adsorptive resins, upper column flow rate is 2.1BV/h, then with the water of 4BV, the 50% of 2BV
Ethanol, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 2.1BV/h, collects 70%
Ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), D101 macroporous adsorbent resin and the DA-of step (3)
The volume ratio of 201 macroporous adsorbent resins is 1:1:1, and height and the diameter ratio of 2 resin columns are all
4:0.8。
Embodiment 6:
A kind of Chinese medicine composition treating acne, including the raw material of Chinese medicine of following masses number:
Radix Et Rhizoma Rhei extract 6 parts, Herba Taraxaci extract 8 parts.
A kind of Chinese medicine composition treating acne, preparation method is: Radix Et Rhizoma Rhei extract and Pu are public
Peroral dosage form described on any pharmaceutics is made after English extract mix homogeneously.
The extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 65 DEG C of the acetone adding 6 times of quality is heated to reflux 65 minutes, mistake
Filter, obtains filtrate；
(2) the 1/4 of filtrate decompression recycling design to volume, concentrated solution is obtained, then with concentrating
The petroleum ether extraction of 2.5 times of volumes of liquid, obtains petroleum ether part；
(3) DM-in petroleum ether part
301 macroporous adsorptive resins, upper column flow rate is 2.1BV/h, then by the methanol-eluted fractions of 3BV, washes
Separation of flow speed is 2.1BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.08, is Radix Et Rhizoma Rhei and carries
Take thing
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C.
Wherein, petroleum ether and DM-in step (3)
The volume ratio of 301 macroporous adsorbent resins is 1:1, and the height of resin column and diameter are than for 4:1.
The extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 85 DEG C of heating and refluxing extraction 110 of 8 times of quality 90% ethanol
Minute, filter, obtain filtrate；
(2) upper for filtrate DA-
201 macroporous adsorptive resins, upper column flow rate is 1.6BV/h, by the water of 4BV, 50% second of 2BV
Alcohol, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1.6BV/h, collects 50% second
Alcohol eluen；
(3) 50% ethanol elution regulation pH to 5-
6, upper D101 macroporous adsorptive resins, upper column flow rate is 1.9BV/h, then with the water of 4BV,
50% ethanol of 2BV, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 1.9BV/h,
Collect 70% ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci
Extract.
Wherein, the filtrate of step (2), DA-
The volume ratio of the D101 macroporous adsorbent resin of 201 macroporous adsorbent resins and step (3) is 1:1:1
, the height of 2 resin columns and diameter are 4:0.8 than all.
Embodiment 7:
Using conventional fabrication process, mixed extract of the present invention can be made into any pharmaceutics
Upper described peroral dosage form, such as tablet, capsule, granule, pill, syrup.
Remaining is with any one in embodiment 1-6.
Embodiment 8:
Will be by embodiment 1-
The extract of 3 mix homogeneously prepared, according to the operational approach of conventional tablet, adds medicinal auxiliary
Material, by each component mix homogeneously, wet granulation, sieves, and uses conventional tablet to prepare method pressure
Sheet makes tablet, and the content of the most each component is as follows:
Remaining is with any one in embodiment 1-6.
Embodiment 9:
Will be by embodiment 1-
The extract of 3 mix homogeneously prepared, according to the operational approach of conventional capsule, adds medicinal
Adjuvant, by each component mix homogeneously, pelletizes, and uses conventional capsule to prepare method filling and makes
Capsule, the content of the most each component is as follows:
Remaining is with any one in embodiment 1-6.
Embodiment 10:
Will be by embodiment 1-
The extract of 3 mix homogeneously prepared, according to the operational approach of conventional syrup, ethanol precipitates
1-
2 times, filtering, concentrate, aqueous precipitation, filter, add pharmaceutic adjuvant, filter, subpackage goes out
Bacterium, uses conventional syrup to prepare method and prepares oral liquid, and the content of the most each component is as follows:
Remaining is with any one in embodiment 1-6.
Animal clinical study:
On the impact of family's rabbit ear acne caused by coal tar:
Take new zealand rabbit 48, male and female half and half, be divided into 8 groups by body weight and sex Stochastic Equilibrium,
I.e. Normal group, model control group, embodiment 1-
6 groups, often group 6.After packet in addition to Normal group, remaining is respectively organized all in auris dextra medial surface
Syrinx opening part 3cm × 3cm scope, is coated with dense liquor carbonis detergens 1 time every day, and each 0.6
Ml, continuous 4 weeks, Normal group rabbit smeared olive oil 0.6ml at same position.4 weeks
After, smear coal tar person all has ear to thicken, coarse, black keratotic plug etc. changes.Embodiment 1-
6 groups start by gastric infusion respectively, and administration volume is 2g/kg, Normal group, model pair
Distilled water 5g/kg is given with method gavage according to group, every day 1 time, continuous 2 weeks.It is administered simultaneously by front
State modeling method and continue modeling.Every El, in addition to being administered, answers local at perusal rabbit ear modeling
The change of skin, ear thickness, hardness, rough degree, follicular orifice are with or without black keratotic plug etc..Inspection
Survey index: 1. ear acne formed intensity: last be administered after 1
H, measures each group of rabbit left and right ear same position thickness with spiral micrometer, subtracts with auris dextra thickness
The difference removing left ear thickness forms intensity as acne；2. ear acne response strength: after measurement
With 10
Each group of rabbit left and right ear same position auricle laid by mm metal card punch, weighs, with auris dextra sheet
Quality deducts left auricle quality as acne response strength；3. pathological observation: stay after weighing
Each group rabbit right auricle, 10% formaldehyde is fixed, and carries out pruning, embedding by pathological examination program
, section, HE dyeing, mounting etc., last microscopy, part specimen photomicrograph, observer
Rabbit auris dextra tissue epidermis, high dermis iuntercellular and cellular morphology change, cell infiltration etc.
Abnormal change.
Experimental acne histology's criterion :-
For without acne；+ for the slight keratinization of hair follicle, hypertrophy that sebaceous gland is slight or without obvious hypertrophy；++ for
Hair follicle area slightly expands, and the keratinization material of moderate seen from infundibulum, sebaceous gland slightly increases
Raw；+++ substantially expanding for hair follicle area, inside have substantial amounts of keratinization material, sebaceous gland diameter is bright
Aobvious increase.
Table 1 present invention acne experimental on the rabbit ear histological impact of pathology
Table 2 present invention is on the impact of family's rabbit ear acne caused by coal tar
Result shows: in embodiment of the present invention 1-
The shadow of family's rabbit ear acne caused by the 3 acne histological impacts of pathology experimental on the rabbit ear and coal tar
Ring and be all substantially better than embodiment 4 groups (technological parameter is different), 5 groups of (carrying of Radix Et Rhizoma Rhei of embodiment
Taking technique is different), embodiment 6 groups (extraction process of Herba Taraxaci is different), it is seen that technique is joined
Number, extraction process all effective ingredient and treatments to prepared extract drugs of Radix Et Rhizoma Rhei and Herba Taraxaci
Effect has an impact, and therefore present invention employs carrying of specific technological parameter, Radix Et Rhizoma Rhei and Herba Taraxaci
Taking technique, could prepare the medicine for the treatment of acne evident in efficacy.
Claims (6)
1. treat a Chinese medicine composition for acne, it is characterized by: include following traditional Chinese medicine raw: Radix Et Rhizoma Rhei, Herba Taraxaci.
2. treat a Chinese medicine composition for acne, it is characterized by: include the raw material of Chinese medicine of following masses number: Radix Et Rhizoma Rhei extract 3-7 part, Herba Taraxaci extract 7-9 part.
A kind of Chinese medicine composition treating acne, is characterized by: preparation method is: make peroral dosage form described on any pharmaceutics after Radix Et Rhizoma Rhei extract and Herba Taraxaci extract mix homogeneously.
A kind of Chinese medicine composition treating acne, is characterized by: the extracting method of described Radix Et Rhizoma Rhei extract is:
(1) being pulverized by Radix Et Rhizoma Rhei, 60-70 DEG C of the acetone adding 5-7 times of quality is heated to reflux 50-70 minute, filters, obtains filtrate；
(2) the 1/5-1/3 of filtrate decompression recycling design to volume, obtain concentrated solution, then the petroleum ether extraction with 2-3 times of volume of concentrated solution, obtain petroleum ether part；
(3) DM-301 macroporous adsorptive resins in petroleum ether part, upper column flow rate is 1.8-2.2BV/h, then by the methanol-eluted fractions of 3BV, elution flow rate is 1.8-2.2BV/h, collects eluent；
(4) eluent is evaporated to the extractum that relative density is 1.05-1.09, is Radix Et Rhizoma Rhei extract；
Wherein, in step (2), the boiling range specification of petroleum ether is 60-90 DEG C；
Wherein, in step (3), the volume ratio of petroleum ether and DM-301 macroporous adsorbent resin is 1:1, and the height of resin column and diameter are than for 4:1.
A kind of Chinese medicine composition treating acne, is characterized by: the extracting method of described Herba Taraxaci extract is:
(1) Herba Taraxaci is pulverized, add 7-9 times of quality 50% 80-90 DEG C of heating and refluxing extraction 80-120 minute of ethanol, filter, obtain filtrate；
(2) upper for filtrate D101 macroporous adsorptive resins, upper column flow rate is 1.4-1.6BV/h, and with the water of 4BV, 50% ethanol of 2BV, the 95% ethanol gradient elution successively of 1BV, elution flow rate is 1.4-1.6BV/h, collects 50% ethanol elution；
(3) 50% ethanol elution regulation pH to 5-6, upper DA-201 macroporous adsorptive resins, upper column flow rate is 1.8-2.2BV/h, again with the water of 4BV, 50% ethanol of 2BV, the 70% ethanol gradient elution successively of 1BV, elution flow rate is 1.8-2.2BV/h, collects 70% ethanol elution；
(4) 70% ethanol elution decompression and solvent recovery to the 1/2 of volume, it is Herba Taraxaci extract；
Wherein, the volume ratio of the DA-201 macroporous adsorbent resin of the filtrate of step (2), D101 macroporous adsorbent resin and step (3) is 1:1:1, and the height of 2 resin columns and diameter are 4:0.8 than all.
A kind of Chinese medicine composition treating acne, is characterized by: described peroral dosage form is: tablet, capsule, granule, pill, syrup.
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