CN109498663A - Nine primary and secondary genus plants extracts and its pharmaceutical composition preparation method and antimalarial purposes - Google Patents
Nine primary and secondary genus plants extracts and its pharmaceutical composition preparation method and antimalarial purposes Download PDFInfo
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- CN109498663A CN109498663A CN201811610666.3A CN201811610666A CN109498663A CN 109498663 A CN109498663 A CN 109498663A CN 201811610666 A CN201811610666 A CN 201811610666A CN 109498663 A CN109498663 A CN 109498663A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A61P33/06—Antimalarials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to nine primary and secondary genus plants extracts to prepare the application in anti-malarial product, belongs to Chinese medicine, natural drug pharmaceutical field.Using extract of the present invention as antimalarial drug of active ingredient.The present invention has expanded the indication of nine primary and secondary platymiscium medicinal materials, improves the medical value of nine primary and secondary platymiscium medicinal materials.
Description
Technical field:
The invention belongs to Chinese medicines, natural drug pharmaceutical field, and in particular, to the preparation method of nine primary and secondary genus plants extracts and
Using the extract as the pharmaceutical composition of active ingredient, and its application in antimalarial agent.
Technical background:
Nine primary and secondary categories (Dobinea) plant belongs to Anacardiaceae (Anacardiaceae) or nine primary and secondary sections (Podoaceae), to fill
The wooden or perennial suffruticose draft for having loose rhizomes.The whole world has 2 kinds, be Dobinea delavayi (Dobinea delavayi
(Baill.) Baill.) nine primary and secondary of He Gongshan (D. vulgarisBuch.-Ham. ex D. Don), China produces.Goat's horn
Rhizoma Gastrodiae be perennial suffruticose draft, rhizomes is coarse, because process after shaped like goat's horn due to gain the name；Central Yunnan is produced to northwest
Portion, South-west Sichuan；It is born in 1100-2300 meters of height above sea level of careless slope on the sunny side or shrubbery.It is civil to be damaged for cough with lung heat, bruise
Hurt, fracture and treat parotitis, mazoitis, boil, dizziness, rheumatism etc., there is the benefits of anti-inflammatory analgetic, relaxing tendons and activating collaterals.In recent years,
In terms of only a small amount of Dobinea delavayi chemical component research report (Chemistry of Natural Compounds, 2013,
49(1): 46-48; Planta Medica, 2012, 78(17): 1878-1880; Chinese herbal medicine, 2012, 43(10):
1916-1919; Anhui agronomy notification, 2011, 17(19): 35-36; Guangxi plant, 1995, 15(3): 252-
253.).Nine primary and secondary of tribute mountain is shrub, 1-3 meters high；Northwestern Yunnan Province and Southeastern Tibet are produced, is born in 1350-1700 meters of height above sea level
Along the river, in river valley sparse woods.Its medicinal record that so far there are no and other activity research report.
Currently, having no the preparation method of nine primary and secondary genus plants extracts both at home and abroad and using the extract as the medicine of active ingredient
Object combination, and its report applied in anti-malarial.
Summary of the invention:
The present invention is intended to provide the preparation method of nine primary and secondary genus plants extracts and using it as the pharmaceutical composition of active ingredient, with
And they are preparing the application in antimalarial agent.
Above-mentioned purpose of the invention is achieved by following technical solution:
The preparation method of nine primary and secondary genus plants extracts takes the root of nine primary and secondary platymisciums or complete stool to dry, pulverize 20-30 mesh,
It is extracted 2-5 times at room temperature with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water,
Each 12-72 h, combined extract, extracting solution are concentrated to give medicinal extract.Or the root of nine primary and secondary platymisciums or complete stool dry, pulverize
20-30 mesh, with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water 80-120oC condition
Lower refluxing extraction 2-5 times, each 2-8 h, combined extract, extracting solution is concentrated to give medicinal extract.Or by the root of nine primary and secondary platymisciums or
Complete stool dry, pulverize 20-30 mesh, with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water
It is extracted 2-5 times under the supplementary means such as ultrasound or microwave, each 10-150 min, combined extract, extracting solution is concentrated under reduced pressure
Obtain medicinal extract.It is every time 5-25 times for impregnating dregs of a decoction weight with quantity of solvent when extraction.
Nine primary and secondaries platymiscium of the present invention be Dobinea delavayi (Dobinea delavayi(Baill.) Baill.)
With nine primary and secondary of tribute mountain (D. vulgarisBuch.-Ham. ex D. Don).
Antimalarial agent contains nine primary and secondary genus plants extracts and conventional adjuvant.
Pharmaceutical composition, wherein nine primary and secondary genus plants extracts and pharmaceutically acceptable load containing therapeutically effective amount
Body.
Nine primary and secondary genus plants extracts are preparing the application in antimalarial agent.
The present invention is used for antimalarial pharmaceutical composition, wherein containing nine primary and secondary genus plants extracts and pharmaceutically acceptable
Carrier.
Pharmaceutically acceptable carrier described in pharmaceutical composition of the present invention refers to the pharmaceutical carrier of pharmaceutical field routine.This
Inventing nine primary and secondary genus plants extracts can be in the form of compositions by way of oral, nasal inhalation, rectum or parenteral administration
It is applied to the patient for needing this treatment.When for taking orally, conventional solid pharmaceutical preparation such as tablet, pulvis, particle can be made into
Liquid preparation such as oil-suspending agent, syrup, elixir etc. is made in agent, capsule etc.；When for parenteral administration, injection can be made into
Solution etc..Preferred form is tablet, capsule and injection.
The various dosage forms of pharmaceutical composition of the present invention can be prepared according to the conventional production process of pharmaceutical field.Such as make to live
Sexual element is mixed with one or more carriers, is then made into required dosage form.
Pharmaceutical composition of the invention preferably comprises the active ingredient that weight ratio is 0.1% -99.5%, most preferably weight
Than the active ingredient for 0.5% -95%.
The amount of application of nine primary and secondary genus plants extracts of the invention can according to route of administration, the age of patient, weight, treated
Disease type and the variation such as severity, daily dose can be 0.1-100 mg/kg weight, preferably 0.5-50 mg/kg
Weight.It one or many can apply.
Nine primary and secondary genus plants extracts of the invention show preferable anti-malarial activity.
The present invention has carried out anti-malarial activity screening to nine primary and secondary genus plants extracts, and nine primary and secondary genus plants extracts are shown
Preferable antimalarial active.In antimalarial active application, nine primary and secondary genus plants extracts are to be applied to substrate or one with following amount
On population, the range 0.01-30 g of the amount is optionally combined with carrier and/or media preferably in 0.03-10 g.
Specific embodiment:
Essentiality content of the invention is further illustrated with the embodiment of the present invention below, those skilled in the art can be made more comprehensively
Ground understands the present invention, but do not limit the invention in any way.
Embodiment 1:
The preparation of Dobinea delavayi extract of the present invention:
The root of Dobinea delavayi plant is spontaneously dried, 20-30 mesh is crushed to, with 75% ethyl alcohol 95oReflux mentions 3 times under the conditions of C,
2 h every time, extracting solution merge, and extracting solution is concentrated under reduced pressure and obtains medicinal extract.
Embodiment 2:
The anti-malarial activity of extract of the present invention detects:
Using 4 days international Inhibition test methods, every mouse peritoneal inoculation 1 × 107The red blood cell of a Infected With Plasmodium, connects
Gastric infusion after kind 3 h of plasmodium, is administered once every 24 h later, and (inoculation day is D to successive administration within 4 days0, next day D1, according to
This analogizes), in the 5th day (D4) tail vein takes blood, thin smear film is coated, methanol is fixed, Rui Shi-Jim Sa (Wright-Giemsa)
Mixed staining dyeing, 10 × 100 microscopes oil is under the microscope.Do not find that plasmodium is asexual if being checked behind 50 visuals field at random
Body, then be determined as feminine gender, positive thin smear film piece then 5 visuals field of random counter, and Erythrocytes are no less than 1000, then press
Plasmodium inhibiting rate is calculated according to following formula.
Infected With Plasmodium rate (%)=infection plasmodium red blood cell/Erythrocytes × 100%
Plasmodium inhibiting rate (%)=[(control group average rate sample sets average rate)/control group average rate]
×100%
Activity data is shown in Table 1.
1 Dobinea delavayi extract of table antimalarial active data (n=4)
Claims (8)
1. the preparation method of nine primary and secondary genus plants extracts, which is characterized in that with organic solvent petroleum ether or chloroform or acetic acid second
The root or complete stool of ester or acetone or methanol or ethyl alcohol or the direct cold soaking of water or nine primary and secondary platymiscium of circumfluence distillation, are extracted
Liquid by extracting solution concentration, is dried to obtain nine primary and secondary genus plants extracts.
2. the preparation method of extract described in claim 1, it is characterised in that the root of nine primary and secondary platymisciums or complete stool is dry, powder
It is broken to arrive 20-30 mesh, with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water in room temperature condition
Lower extraction, extracting solution are concentrated to give medicinal extract.
3. the preparation method of extract described in claim 1, it is characterised in that the root of nine primary and secondary platymisciums or complete stool is dry, powder
It is broken to arrive 20-30 mesh, with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or hydro-thermal refluxing extraction,
Extracting solution is concentrated to give medicinal extract.
4. the preparation method as described in claim 2 and 3, which is characterized in that, can be in ultrasound or microwave when immersion or heat reflux
Etc. extract under supplementary means, be every time 5-25 times for impregnating dregs of a decoction weight with quantity of solvent.
5. preparation method according to claim 1 to 3, it is characterised in that: the nine primary and secondary platymisciums are Dobinea delavayi
(Dobinea delavayi(Baill.) Baill.) nine primary and secondary of He Gongshan (D. vulgaris Buch.-Ham. ex D.
Don).
6. pharmaceutical composition, wherein extract and pharmaceutically acceptable carrier described in the claim 1 containing therapeutically effective amount.
7. antimalarial agent, wherein containing extract and conventional adjuvant described in claim 1.
8. extract described in claim 1 is preparing the application in antimalarial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811610666.3A CN109498663A (en) | 2018-12-27 | 2018-12-27 | Nine primary and secondary genus plants extracts and its pharmaceutical composition preparation method and antimalarial purposes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811610666.3A CN109498663A (en) | 2018-12-27 | 2018-12-27 | Nine primary and secondary genus plants extracts and its pharmaceutical composition preparation method and antimalarial purposes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109498663A true CN109498663A (en) | 2019-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811610666.3A Pending CN109498663A (en) | 2018-12-27 | 2018-12-27 | Nine primary and secondary genus plants extracts and its pharmaceutical composition preparation method and antimalarial purposes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109912551A (en) * | 2019-04-08 | 2019-06-21 | 大理大学 | Sesquiterpene eudesmanolide class compound and its pharmaceutical composition and preparation method and application |
| CN110305017A (en) * | 2019-08-09 | 2019-10-08 | 大理大学 | Sesquiterpene eudesmanolide class compound and its pharmaceutical composition and preparation method and application are dropped |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126601A (en) * | 1995-01-14 | 1996-07-17 | 冯成林 | Fracture setting medicine |
-
2018
- 2018-12-27 CN CN201811610666.3A patent/CN109498663A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126601A (en) * | 1995-01-14 | 1996-07-17 | 冯成林 | Fracture setting medicine |
Non-Patent Citations (2)
| Title |
|---|
| 沈怡等: "羊角天麻化学成分及β-羟高铁血红素形成抑制活性研究", 《天然产物研究与开发》 * |
| 程忠泉等: "羊角天麻化学成分的研究", 《中草药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109912551A (en) * | 2019-04-08 | 2019-06-21 | 大理大学 | Sesquiterpene eudesmanolide class compound and its pharmaceutical composition and preparation method and application |
| CN109912551B (en) * | 2019-04-08 | 2023-04-11 | 大理大学 | Eudesmane sesquiterpene compound and pharmaceutical composition, preparation method and application thereof |
| CN110305017A (en) * | 2019-08-09 | 2019-10-08 | 大理大学 | Sesquiterpene eudesmanolide class compound and its pharmaceutical composition and preparation method and application are dropped |
| CN110305017B (en) * | 2019-08-09 | 2022-02-25 | 大理大学 | Noreudesmane sesquiterpenoids, and pharmaceutical composition, preparation method and application thereof |
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Application publication date: 20190322 |
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