CN109718263A - Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation method and application - Google Patents
Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation method and application Download PDFInfo
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- CN109718263A CN109718263A CN201811610706.4A CN201811610706A CN109718263A CN 109718263 A CN109718263 A CN 109718263A CN 201811610706 A CN201811610706 A CN 201811610706A CN 109718263 A CN109718263 A CN 109718263A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention relates to Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation methods and application, belong to Chinese medicine, natural drug pharmaceutical field.Using extract of the present invention as antimalarial drug of active ingredient, preparation method takes Megarpaea delavayi Franch platymiscium complete stool to dry, pulverize 20-30 mesh, is extracted with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water.Megarpaea delavayi Franch genus plants extract of the present invention prepares the application in antimalarial agent: being applied on substrate or a population with the amount of 0.01-30 g, is optionally combined with carrier and/or media, has preferable anti-malarial activity.The present invention has expanded the indication of Megarpaea delavayi Franch platymiscium medicinal material, improves the medical value of Megarpaea delavayi Franch platymiscium medicinal material.
Description
Technical field:
The invention belongs to Chinese medicines, natural drug pharmaceutical field, and in particular, to the preparation method of Megarpaea delavayi Franch genus plants extract and
Using the extract as the pharmaceutical composition of active ingredient, and its application in antimalarial agent.
Technical background:
Megarpaea delavayi Franch category (Megacarpaea) plant belongs to Cruciferae (Cruciferae), it is perennial sturdy draft, often has very
Thick root.The whole world has 7 kinds, is mainly distributed on the Central Asia and Himalaya Region.There are 3 kinds of (Megarpaea delavayi Franch, more stamen Megarpaea delavayi Franch and big in China
Fruit Megarpaea delavayi Franch), 4 mutation and 1 modification.Wherein Megarpaea delavayi Franch (Megacarpaea delavayiIt Franch.) is Dali famous product
One of；Herb is medicinal, there is heat-clearing effect；Also salt down and make salted vegetables.In Dali, Bai nationality people think its with heat-clearing, help digestion it is strong
Stomach effect, is usually used in controlling that dysentery, cough with lung heat, stomach energy be stagnant, indigestion.In recent years, only verifying Megarpaea delavayi Franch is medicinal on a small quantity
With the research of its pharmacognosy and Related Component report (China National folk medicine, 2009, 18(5): 1-3; Dali College Report, 2009, 8(2): 8-10; Yunnan traditional Chinese medicine magazine, 2006, (3): 52; China National folk medicine magazine,
2006, (2): 113-114+124; National medicine magazine, 2005, (4): 18; Chinese experimental pharmacology of traditional Chinese medical formulae magazine,
2013, 19(10): 46-49; Agriculture of Anhui science, 2011,39 (4): 2087-2088.), have no other activity research
Report.
Currently, having no the preparation method of Megarpaea delavayi Franch genus plants extract both at home and abroad and using the extract as the medicine of active ingredient
Object combination, and its report applied in anti-malarial.
Summary of the invention:
The present invention is intended to provide the preparation method of Megarpaea delavayi Franch genus plants extract and using it as the pharmaceutical composition of active ingredient, with
And they are preparing the application in antimalarial agent.
Above-mentioned purpose of the invention is achieved by following technical solution:
The preparation method of Megarpaea delavayi Franch genus plants extract takes Megarpaea delavayi Franch platymiscium complete stool to dry, pulverize 20-30 mesh, and use is organic
Solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water extract 2-5 times at room temperature, each 12-
Medicinal extract is concentrated under reduced pressure to obtain in 72 h, combined extract, extracting solution.Or Megarpaea delavayi Franch platymiscium complete stool be dry, pulverize into 20-30 mesh,
With organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water 80-120oReflux mentions under the conditions of C
It takes 2-5 times, each 2-8 h, combined extract, medicinal extract is concentrated under reduced pressure to obtain in extracting solution.Or dry, the powder by Megarpaea delavayi Franch platymiscium complete stool
It is broken to arrive 20-30 mesh, with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water in ultrasound or
It is extracted 2-5 times under the supplementary means such as microwave, each 10-150 min, combined extract, medicinal extract is concentrated under reduced pressure to obtain in extracting solution.It extracts
When, it is every time 5-25 times for impregnating dregs of a decoction weight with quantity of solvent.
Megarpaea delavayi Franch platymiscium of the present invention be more stamen Megarpaea delavayi Franch (Megacarpaea polyandra Benth. ex
Madden) and Megarpaea delavayi Franch (M. delavayiFranch.) and its long valve Megarpaea delavayi Franch of mutation (M. delavayi Franch.
Var. grandiflora O. E. Schulz), short plumage split Megarpaea delavayi Franch (M. delavayi Franch. var.
Pinnatifida P. Danguy), short Megarpaea delavayi Franch (M. delavayiFranch. var. minor W. W. Smith),
Leaflet Megarpaea delavayi Franch (M. delavayi Franch. var. minor W. W. Smith form. microphylla O. E.
Schulz) and grey violet flower Megarpaea delavayi Franch (M. delavayi Franch. var. minor W. W. Smith form.
Pallidiflora O. E. Schulz).
Antimalarial agent contains Megarpaea delavayi Franch genus plants extract and conventional adjuvant.
Pharmaceutical composition, wherein Megarpaea delavayi Franch genus plants extract and pharmaceutically acceptable load containing therapeutically effective amount
Body.
Megarpaea delavayi Franch genus plants extract is preparing the application in antimalarial agent.
The present invention is used for antimalarial pharmaceutical composition, wherein containing Megarpaea delavayi Franch genus plants extract and pharmaceutically acceptable
Carrier.
Pharmaceutically acceptable carrier described in pharmaceutical composition of the present invention refers to the pharmaceutical carrier of pharmaceutical field routine.This
Invention Megarpaea delavayi Franch genus plants extract can be in the form of compositions by way of oral, nasal inhalation, rectum or parenteral administration
It is applied to the patient for needing this treatment.When for taking orally, conventional solid pharmaceutical preparation such as tablet, pulvis, particle can be made into
Liquid preparation such as oil-suspending agent, syrup, elixir etc. is made in agent, capsule etc.；When for parenteral administration, injection can be made into
Solution etc..Preferred form is tablet, capsule and injection.
The various dosage forms of pharmaceutical composition of the present invention can be prepared according to the conventional production process of pharmaceutical field.Such as make to live
Sexual element is mixed with one or more carriers, is then made into required dosage form.
Pharmaceutical composition of the invention preferably comprises the active ingredient that weight ratio is 0.1% -99.5%, most preferably weight
Than the active ingredient for 0.5% -95%.
The amount of application of Megarpaea delavayi Franch genus plants extract of the present invention can according to route of administration, the age of patient, weight, treated
Disease type and the variation such as severity, daily dose can be 0.1-100 mg/kg weight, preferably 0.5-50 mg/kg
Weight.It one or many can apply.
Megarpaea delavayi Franch genus plants extract of the invention shows preferable anti-malarial activity.
The present invention has carried out anti-malarial activity screening to Megarpaea delavayi Franch genus plants extract, and Megarpaea delavayi Franch genus plants extract is shown
Preferable antimalarial active.In antimalarial active application, Megarpaea delavayi Franch genus plants extract is to be applied to substrate or one with following amount
On population, the range 0.01-30 g of the amount is optionally combined with carrier and/or media preferably in 0.03-10 g.
Specific embodiment:
Essentiality content of the invention is further illustrated with the embodiment of the present invention below, those skilled in the art can be made more comprehensively
Ground understands the present invention, but do not limit the invention in any way.
Embodiment 1:
The preparation of Megarpaea delavayi Franch extract of the present invention:
Megarpaea delavayi Franch plant complete stool is dried in the shade naturally, is crushed to 30 mesh, with 75% ethyl alcohol 95oReflux mentions 3 times under the conditions of C, and every time 2
H, extracting solution merge, and extracting solution is concentrated under reduced pressure and obtains medicinal extract.
Embodiment 2:
The anti-malarial activity of extract of the present invention detects:
Using 4 days international Inhibition test methods, every mouse peritoneal inoculation 1 × 107The red blood cell of a Infected With Plasmodium, connects
Gastric infusion after kind 3 h of plasmodium, is administered once every 24 h later, and (inoculation day is D to successive administration within 4 days0, next day D1, according to
This analogizes), in the 5th day (D4) tail vein takes blood, thin smear film is coated, methanol is fixed, Rui Shi-Jim Sa (Wright-Giemsa)
Mixed staining dyeing, 10 × 100 microscopes oil is under the microscope.Do not find that plasmodium is asexual if being checked behind 50 visuals field at random
Body, then be determined as feminine gender, positive thin smear film piece then 5 visuals field of random counter, and Erythrocytes are no less than 1000, then press
Plasmodium inhibiting rate is calculated according to following formula.
Infected With Plasmodium rate (%)=infection plasmodium red blood cell/Erythrocytes × 100%
Plasmodium inhibiting rate (%)=[(control group average rate sample sets average rate)/control group average rate]
×100%
Activity data is shown in Table 1.
1 Megarpaea delavayi Franch extract of table antimalarial active data (n=4)
Claims (6)
1. the preparation method of Megarpaea delavayi Franch genus plants extract, which is characterized in that with organic solvent petroleum ether or chloroform or acetic acid second
Ester or acetone or methanol or ethyl alcohol or the direct cold soaking of water or circumfluence distillation Megarpaea delavayi Franch platymiscium complete stool, obtain extracting solution, will
Extracting solution concentration is dried to obtain Megarpaea delavayi Franch genus plants extract.
2. the preparation method of extract described in claim 1, it is characterised in that dry, pulverize Megarpaea delavayi Franch platymiscium complete stool
20-30 mesh, it is cold at room temperature with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or water
Extraction takes, and extracting solution is concentrated to give medicinal extract.
3. the preparation method of extract described in claim 1, it is characterised in that dry, pulverize Megarpaea delavayi Franch platymiscium complete stool
20-30 mesh is extracted with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethyl alcohol or hydro-thermal refluxing extraction
Liquid is concentrated to give medicinal extract.
4. preparation method described in claim 2 and 3, which is characterized in that, can be in ultrasound or microwave etc. when immersion or heat reflux
It is extracted under supplementary means, is every time 5-25 times for impregnating dregs of a decoction weight with quantity of solvent.
5. preparation method according to claim 1 to 3, it is characterised in that: the Megarpaea delavayi Franch platymiscium is more stamen Megarpaea delavayi Franch
With Megarpaea delavayi Franch and its long valve Megarpaea delavayi Franch of mutation, short plumage split Megarpaea delavayi Franch, short Megarpaea delavayi Franch, leaflet Megarpaea delavayi Franch and grey violet flower Megarpaea delavayi Franch.
6. extract described in claim 1 is preparing the application in antimalarial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811610706.4A CN109718263A (en) | 2018-12-27 | 2018-12-27 | Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811610706.4A CN109718263A (en) | 2018-12-27 | 2018-12-27 | Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109718263A true CN109718263A (en) | 2019-05-07 |
Family
ID=66296539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811610706.4A Pending CN109718263A (en) | 2018-12-27 | 2018-12-27 | Megarpaea delavayi Franch genus plants extract and its pharmaceutical composition preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109718263A (en) |
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2018
- 2018-12-27 CN CN201811610706.4A patent/CN109718263A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| SURESH K. GHIMIRE ET AL.,《: "Ethnobotanical classification and plant nomenclature system of high altitude agro-pastoralists in Dolpo, Nepal", 《JOURNAL OF PLANT SCIENCE》 * |
| 夏从龙: "《大理苍山常见药用植物图鉴》", 30 September 2016, 《云南科技出版社》 * |
| 沈磊等: "高河菜提取物对积滞化热模型大鼠消化液成分的影响", 《中国民族民间医药》 * |
| 肖朝江等: "滇西地区25种药用植物抗疟活性研究", 《中国病原生物学杂志》 * |
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