CN112472778A - Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract - Google Patents
Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract Download PDFInfo
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- CN112472778A CN112472778A CN202011557331.7A CN202011557331A CN112472778A CN 112472778 A CN112472778 A CN 112472778A CN 202011557331 A CN202011557331 A CN 202011557331A CN 112472778 A CN112472778 A CN 112472778A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A61P33/06—Antimalarials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to an ivory extract and a preparation method and application of a pharmaceutical composition thereof, belonging to the field of pharmacy of traditional Chinese medicines and natural medicines. The preparation method of the antimalarial drug taking the extract of the invention as an active component comprises the steps of taking dry whole plant or root of the ivory plant, crushing the dry whole plant or root into 30 meshes, and extracting the crushed plant or root with organic solvent petroleum ether, chloroform, ethyl acetate, acetone, methanol, ethanol or water. The application of the ivy extract in preparing the antimalarial agent comprises the following steps: preferably, the antimalarial activity is obtained by applying the composition to a substrate or a population in an amount of 0.01-30 g, optionally in combination with a carrier and/or vehicle. The invention develops the medicinal value of the ivory.
Description
The technical field is as follows:
the invention belongs to the field of traditional Chinese medicine and natural medicine pharmacy, and particularly relates to a preparation method of an ivory extract, a medicine composition taking the extract as an active ingredient, and application of the ivory extract in an antimalarial agent.
Technical background:
ivory root (rhizome of common Ipomoea)Roscoea purpureaSmith is the genus of Ivorax (Zingibergeraceae) of ZingiberaceaeRoscoea) Plant (CN)Biological, perennial herbs; the plant height is 15-45 cm; the roots are tufted and expanded into spindle shapes; the stem base has 2 scaly sheaths. The ivy is produced in Yunnan, and is also distributed in India, Xijin and Burma; grows under pine forest or in grass bush, and has elevation of 2700-. According to the records of the Chinese materia medica, the similar genus of the ivory is the Dian ivory (R. yunnanensisLoes.) and Tibetan rhizome of AraliaceaeR. tibeticaBat.) has certain medicinal effect, and their roots are bitter in taste and cool in nature, and have the effects of moistening lung, relieving cough, and tonifying deficiency; can be used for treating cough, asthma, asthenia after illness, and edema due to deficiency. At present, a preparation method of an ivory extract, a medicinal composition taking the extract as an active ingredient and a report of application of the ivory extract in malaria resistance are not seen at home and abroad.
The invention content is as follows:
the invention aims to provide a preparation method of an ivory extract, a pharmaceutical composition taking the ivory extract as an active ingredient, and application of the ivory extract and the pharmaceutical composition in preparation of an antimalarial agent.
The above object of the present invention is achieved by the following technical solutions:
a method for preparing Ivory root extract comprises collecting dry whole plant or root of Ivory root, pulverizing to 30 mesh, extracting with organic solvent such as petroleum ether, chloroform, ethyl acetate, acetone, methanol, ethanol or water at room temperature for 12-48 hr for 2-5 times, mixing extractive solutions, and concentrating to obtain extract. Or pulverizing dried whole plant or root of the plant of Ivory to 30 mesh, and extracting with organic solvent such as petroleum ether, chloroform, ethyl acetate, acetone, methanol, ethanol or water at 80-120 deg.CoExtracting under reflux for 2-5 times (each for 2-8 hr), mixing extractive solutions, and concentrating to obtain extract. During soaking or hot reflux, ultrasonic or microwave extraction may be adopted, and the solvent amount is 5-25 times of the weight of the soaked medicine residue.
An antimalarial agent comprises an extract of ivy and conventional adjuvants.
A pharmaceutical composition comprises a therapeutically effective amount of an extract of an ivory and a pharmaceutically acceptable carrier.
Use of extract of ivy root in preparing antimalarial agent is provided.
The invention relates to a pharmaceutical composition for resisting malaria, which comprises an ivory extract and a pharmaceutically acceptable carrier.
The pharmaceutically acceptable carrier in the pharmaceutical composition of the invention refers to a conventional pharmaceutical carrier in the pharmaceutical field. The extract of ivory of the present invention may be administered in the form of a composition by oral, nasal inhalation, rectal or parenteral administration to a patient in need of such treatment. For oral administration, it can be made into conventional solid preparations such as tablet, powder, granule, capsule, etc., and liquid preparations such as oil suspension, syrup, elixir, etc.; for parenteral administration, it can be made into solution for injection, etc. Preferred forms are tablets, capsules and injections.
Various dosage forms of the pharmaceutical composition of the present invention can be prepared according to conventional production methods in the pharmaceutical field. For example, the active ingredient may be combined with one or more carriers and then formulated into the desired dosage form.
The pharmaceutical compositions of the present invention preferably contain 0.1% to 99.5% by weight of the active ingredient, most preferably 0.5% to 95% by weight of the active ingredient.
The amount of the extract of the ivory of the present invention to be administered may vary depending on the route of administration, age, weight of the patient, type and severity of the disease to be treated, etc., and the daily dose thereof may be 0.1 to 100 mg/kg of body weight, preferably 0.5 to 50 mg/kg of body weight. One or more administrations may be carried out.
The ivory extract of the invention shows better antimalarial activity.
The ivory extract is subjected to antimalarial activity screening, and shows good antimalarial activity. In antimalarial applications, the extract of ivory is applied to a substrate or a population in an amount ranging from 0.01 to 30 g, preferably from 0.03 to 10 g, optionally in association with a carrier and/or vehicle.
The specific implementation mode is as follows:
the following examples are provided to further illustrate the essence of the present invention, which will enable a person skilled in the art to more fully understand the invention, but are not intended to limit the invention in any way.
Example 1:
the preparation of the ivory extract comprises the following steps:
collecting dry whole plant of rhizoma anemarrhenae 100 g, pulverizing to 30 mesh, adding 75% ethanol at 95%oExtracting under reflux for 3 times (2 hr each time) under C condition, mixing extractive solutions, and concentrating under reduced pressure to obtain extract 17.1 g with extraction rate of 17.1%.
Example 2:
the antimalarial activity of the extract of the invention is detected:
adopting an international 4-day inhibition experiment method, 1 × 10 percent of the total amount of the active ingredients is inoculated into the abdominal cavity of each mouse7Inoculating plasmodium into erythrocyte, administering intragastrically after 3 hr, and administering once every 24 hr for 4 days (day of inoculation is D)0The next day is D1And so on) at day 5 (D)4) The tail vein was bled, filmed, fixed with methanol, stained by mixed staining with Wright-Giemsa (Wright-Giemsa), and observed under a 10 × 100 microscope under oil microscope. If no plasmodium aneuploidy is found after randomly checking 50 visual fields, the judgment is negative, 5 visual fields are randomly counted on a positive blood film sheet, the total number of red blood cells is not less than 1000, and then the plasmodium inhibition rate is calculated according to the following formula.
Plasmodium infection rate (%). The total number of erythrocytes/erythrocytes x 100% that infected plasmodium
Plasmodium inhibition (%) (mean control mean infection ‒ sample group mean infection)/control mean infection ] × 100%
The activity data are shown in Table 1.
TABLE 1 antimalarial Activity data of the extract of Ipomoea javanica: (n = 4）
Claims (7)
1. The preparation method of the ivory extract is characterized in that the whole plant or root of the ivory plant is directly extracted by using organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethanol or water through cold immersion or hot reflux to obtain extract, and the extract is concentrated and dried to obtain the ivory extract.
2. The method for preparing the extract as claimed in claim 1, wherein the whole plant or root of the plant of the ivory is dried, pulverized to 30 mesh, extracted with organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethanol or water at room temperature, and the extract is concentrated to obtain an extract.
3. The method for preparing the extract as claimed in claim 1, wherein the whole plant or root of the plant of the ivory is dried, pulverized to 30 mesh, extracted by organic solvent petroleum ether or chloroform or ethyl acetate or acetone or methanol or ethanol under hydrothermal reflux, and the extract is concentrated to obtain an extract.
4. The method according to claims 2 and 3, wherein the soaking or thermal refluxing is assisted by ultrasonic or microwave extraction, and the amount of solvent used each time is 5-25 times the weight of the soaked herb residue.
5. A pharmaceutical composition comprising a therapeutically effective amount of the extract of claim 1 and a pharmaceutically acceptable carrier.
6. An antimalarial agent comprising the extract of claim 1 and conventional adjuvants.
7. Use of the extract of claim 1 for the preparation of an antimalarial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011557331.7A CN112472778A (en) | 2020-12-25 | 2020-12-25 | Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011557331.7A CN112472778A (en) | 2020-12-25 | 2020-12-25 | Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract |
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| CN112472778A true CN112472778A (en) | 2021-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011557331.7A Pending CN112472778A (en) | 2020-12-25 | 2020-12-25 | Ivory root extract, preparation method of pharmaceutical composition of ivory root extract and antimalarial application of ivory root extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115869356A (en) * | 2023-01-31 | 2023-03-31 | 大理大学 | Preparation of barb active part with anti-trichina medicine |
-
2020
- 2020-12-25 CN CN202011557331.7A patent/CN112472778A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| ACHARYA BALKRISHNA等: "Astavarga plants–threatened medicinal herbs of the North-West Himalaya", 《INT.J.MED.AROM.PLANTS》 * |
| ANKITA MISRA等: "Nutritional evaluation, antioxidant studies and quantification of poly phenolics, in Roscoea purpurea tubers", 《BMC RESEARCH NOTES》 * |
| 吴寿金等主编: "《现代中草药成分化学》", 31 May 2002, 中国医药科技出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115869356A (en) * | 2023-01-31 | 2023-03-31 | 大理大学 | Preparation of barb active part with anti-trichina medicine |
| CN115869356B (en) * | 2023-01-31 | 2023-12-22 | 大理大学 | Preparation of barb active site for anti-trichina medicine |
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