CN101214278A - New raw material for extracting fructus schizandrae total lignans and preparation technique and use - Google Patents
New raw material for extracting fructus schizandrae total lignans and preparation technique and use Download PDFInfo
- Publication number
- CN101214278A CN101214278A CNA2007101591969A CN200710159196A CN101214278A CN 101214278 A CN101214278 A CN 101214278A CN A2007101591969 A CNA2007101591969 A CN A2007101591969A CN 200710159196 A CN200710159196 A CN 200710159196A CN 101214278 A CN101214278 A CN 101214278A
- Authority
- CN
- China
- Prior art keywords
- extract
- schisandrae chinensis
- gomisin
- fructus schisandrae
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention pertains to the art of medical technology and discloses a novel raw material for Schisandraceae lignans extracted substance and a preparation method thereof. The process takes Chinese medicine Schisandraceae stem (fruit stalk) and Schisandraceae leave as the raw material and adopts one or a plurality of solvent extraction method, resin absorption method, positive-phase column chromatography, reverse-phase column chromatography, etc. after grinding, so as to prepare the extracted substance with 30 to 95 percent of total lignans content. The preparation method has simple process, stable content and good repeatability. The extracted substance can be used as medicine or health food instead of Schisandraceae lignans extracted substance, which makes full use of Schisandraceae resource and releases the short supply of Schisandraceae fruit.
Description
Technical field
The invention belongs to medical technical field, relate to the new raw material and the preparation technology thereof of Fructus Schisandrae Chinensis total lignanoid extract.According to the extract of this preparation technology's gained, the total content of its Lignanoids compounds is at 30-95%.Be used for prevention and treatment hepatitis.
Background technology
The fat-soluble active ingredient of Fructus Schisandrae Chinensis is mainly lignanoid.Its pharmacological action relates to digestive system, cardiovascular system, central nervous system, reproductive system and antioxidation, the anti-ageing various aspects of waiting for a long time (Chinese crude drug the 28th the 2nd phase of volume of February in 2005).
Folium Schisandrae Chinensis and Fructus Schisandrae Chinensis carpopodium (fruit stem) are the dried leaves and the carpopodiums (fruit stem) of Magnoliaceae schisandra plant Radix Schisandrae Bicoloris Schisandra chinensis (Turcz.) Baill., schisandra chinensis Schisandrasphenanthera Rehd.et Wils. and Schizandra other plant.
The foreign scholar uses the CO of high performance liquid chromatograph to Folium Schisandrae Chinensis
2Supercritical extract is analyzed, and finds that it contains the lignanoid identical with fruit of Fructus Schisandrae Chinensis, as (Journal of Chromatography B, 770 (2002) 283289) such as schisandrin, pure second, first element, second element, third element and gomisin N.Contain the lignanoid identical in the Fructus Schisandrae Chinensis carpopodium (fruit stem), as schisandrin, ester first, first element, second element etc. (Yanbian University's medical journal the 28th the 1st phase of volume of March in 2005) with fruit of Fructus Schisandrae Chinensis.
At present, the Fructus Schisandrae Chinensis market demand is very big, do not satisfy the requirement of domestic and international market far away, extinctive cutting having occurred wild resource climingly gathers and destroys, the area that has generally occurs plunderring blue or green phenomenon, makes the Fructus Schisandrae Chinensis resource be subjected in various degree destruction, causes a resource shrinkage of available Fructus Schisandrae Chinensis, downgrade, price are constantly soaring.Fruit was plucked from Cortex et Radix Schisandrae Chinensis with carpopodium (fruit stem) when fruit of Fructus Schisandrae Chinensis was ripe, and carpopodium (fruit stem) is disallowable as impurity, and the Folium Schisandrae Chinensis resource is very abundant especially, if can be with Folium Schisandrae Chinensis and carpopodium (fruit stem) replenishing as Fructus Schisandrae Chinensis lignanoid source, can make full use of resource, turn waste into wealth.
Summary of the invention
The objective of the invention is to provide two kinds of new sources of Fructus Schisandrae Chinensis total lignanoid at above-mentioned schisandra chinensis medicinal material situation in short supply, the preparation technology of two kinds of Fructus Schisandrae Chinensis carpopodiums (fruit stem) and leaf total lignans extract, and the extract and the relevant dosage form thereof that get according to this prepared.As replenishing of Fructus Schisandrae Chinensis resource, substitute fruit of Fructus Schisandrae Chinensis total lignans extract and be used as medicine or health food.
The present invention finishes by following technical solution:
(1), pulverizes decocting in water with Folium Schisandrae Chinensis, carpopodium (fruit stem) drying; With residue drying behind the water extraction, a kind of heating and refluxing extraction of the methanol with 50～95%, ethanol, isopropyl alcohol or n-butyl alcohol, the extracting solution concentrating under reduced pressure is the concentrated solution of density 0.80-1.20; Adopt the macroporous adsorptive resins enrichment, water, 40%～75% ethanol, 95% ethanol elution is collected 95% ethanol elution, the extract that obtains after concentrating, its total lignans weight content is 30%-95%.
(2), pulverize decocting in water with Folium Schisandrae Chinensis, carpopodium (fruit stem) drying; With residue drying behind the water extraction, a kind of heating and refluxing extraction of the methanol with 70～95%, ethanol, isopropyl alcohol or n-butyl alcohol, extracting solution is evaporated to dried, organic solvent room temperature or heating and refluxing extraction, extract adopts the chromatographic column eluting, the extract that obtains behind the eluting, its total lignans weight content is 30%-95%.
According to above prepared and the total lignans extract contain chemical compound: schisandrin, pure second, first element, second element, third element and gomisin G, gomisinL
1, gomisinM
1, gomisinN etc.This extract can mix with acceptable carrier pharmaceutically, makes various useful clinically dosage forms.
Beneficial effect of the present invention
The present invention is directed to and substitute fruit of Fructus Schisandrae Chinensis total lignans extract and be used as medicine or go into health food, make full use of the Fructus Schisandrae Chinensis resource, alleviate the fruit of Fructus Schisandrae Chinensis situation that supply falls short of demand.
The specific embodiment
The following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Chinese cotyledon 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 14L80% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and concentrating under reduced pressure, density is 0.93 concentrated solution 610g.Use the macroporous adsorptive resins enrichment, the difference water, 60% ethanol, 90% ethanol gradient elution, collect 90% ethanol elution, decompression recycling ethanol is used the macroporous adsorptive resins enrichment once more, respectively water, 75% ethanol, 90% ethanol elution is collected 90% ethanol elution, and decompression recycling ethanol obtains the 15g extract.Total lignans content 60%.
Embodiment 2:
Chinese cotyledon 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 14L80% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and decompression recycling ethanol obtain dry extract 130g.With petroleum ether reflux, extract, 3 times, merge extractive liquid,, be evaporated to small size 10ml, by the silica gel column chromatography enrichment, use petroleum ether: behind ethyl acetate (20: the 1) eluting, the reuse petroleum ether: ethyl acetate (2: 1) eluting, collect petroleum ether: ethyl acetate (2: 1) eluent, concentrating under reduced pressure obtains the 10g extract.Total lignans content 65%.
Embodiment 3:
Radix Schisandrae Bicoloris carpopodium (fruit stem) 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 12L90% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and concentrating under reduced pressure, density is 0.91 concentrated solution 650g.Use the macroporous adsorptive resins enrichment, the difference water, 70% ethanol, 90% ethanol gradient elution is collected 90% ethanol elution, and decompression recycling ethanol obtains the 20g extract.Total lignans content 63%.
Embodiment 4:
Radix Schisandrae Bicoloris carpopodium (fruit stem) 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 12L90% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and decompression recycling ethanol obtain dry extract 150g.With petroleum ether reflux, extract, 3 times, merge extractive liquid,, be evaporated to small size 10ml, by the silica gel column chromatography enrichment, use petroleum ether: behind ethyl acetate (20: the 1) eluting, the reuse petroleum ether: ethyl acetate (2: 1) eluting, collect petroleum ether: ethyl acetate (2: 1) eluent, concentrating under reduced pressure obtains the 15g extract.Total lignans content 70%.
Embodiment 5:
Radix Schisandrae Bicoloris carpopodium (fruit stem) 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 12L90% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and decompression recycling ethanol obtain dry extract 150g.With petroleum ether reflux, extract, 3 times, merge extractive liquid,, be evaporated to small size 10ml, silica gel column chromatography, use petroleum ether: behind ethyl acetate (100: 1～1: the 100) gradient elution, merge the same composition eluate, through method purification such as ODS post, high performance liquid chromatogram (HPLC), recrystallization, obtained schisandrin, pure second, first element, second element, third element, gomisin G, gomisinL again
1, gomisinM
1, 25 chemical compounds such as gomisinN.And use nuclear magnetic resonance, NMR (NMR), mass spectrum (MS), infrared spectrum means such as (IR) to determine their structure.
Embodiment 6:
Embodiment 1,2 resulting Folium Schisandrae Chinensis total lignans extracts resulting chemical compound in thin layer chromatography, high performance liquid chromatogram (HPLC) and embodiment 5 is compared, show that it contains schisandrin, pure second, first element, second element, third element, gomisinL
1, gomisinM
1, 14 kinds of lignanoids such as gomisinN.
Embodiment 7:
Radix Schisandrae Bicoloris carpopodium (fruit stem) 2Kg pulverizes, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 12L90% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter, merging filtrate and concentrating under reduced pressure, density is 0.91 concentrated solution 650g.Use the macroporous adsorptive resins enrichment, the difference water, 70% ethanol, 90% ethanol gradient elution is collected 90% ethanol elution, and decompression recycling ethanol obtains the 20g extract.Total lignans content 63%.Add 4g starch, mixing, 80% alcohol granulation, drying adds magnesium stearate 0.3g, mixing, the capsule of packing into No. 1 promptly gets 70 of the hard capsules that the present invention makes.Every contains content 0.3g.
Embodiment 8: the Fructus Schisandrae Chinensis total lignanoid extract is to the therapeutical effect of carbon tetrachloride hepatic injury mice
Get 50 of mices, be divided into 5 groups at random, be respectively normal control group, carbon tetrachloride (CCl
4) model group, fruit of Fructus Schisandrae Chinensis total lignans extract group, embodiment 1 extract group and embodiment 3 extract groups.
Wherein fruit of Fructus Schisandrae Chinensis total lignans preparation method of extract is as follows: the Radix Schisandrae Bicoloris fruit is pulverized, and adds water reflux, extract, twice, keeps slight boiling condition 1.5 hours at every turn, filters, and discards the filtrate of merging.After residue dries, add 90% ethanol, heating and refluxing extraction 3 times, each 2 hours, filter the macroporous adsorptive resins enrichment of merging filtrate and concentrating under reduced pressure, concentrated solution, the difference water, 40% ethanol, 90% ethanol gradient elution is collected 90% ethanol elution, decompression recycling ethanol, decompression recycling ethanol obtain the extract of total lignans content 70%.
Animal experiment method is as follows: except that the normal control group, and every group of first day lumbar injection 0.1%CCl
4Paraffin oil solution (10ml/kg), simultaneously continuous gastric infusion 7 days, 16 hours eye sockets are got blood after the last administration, survey serum glutamic pyruvic transminase (SGPT).
Experimental result sees the following form:
| Group (n=10) | Dosage (mg/kg) | SGPT content (U/ml) |
| The normal control group | 168.1±27.34 | |
| CCl 4Model group | 443.2±47.61 | |
| Fruit of Fructus Schisandrae Chinensis total lignans extract group | 200 | 271.3±35.53 |
| Embodiment 1 extract group | 200 | 281.4±34.79 |
| Embodiment 3 extract groups | 200 | 287.7±36.88 |
Conclusion: CCl4 group mice SGPT is significantly higher than normal control group (P＜0.01); Compare with the CCl4 group, fruit of Fructus Schisandrae Chinensis total lignans extract group, embodiment 1 extract group, embodiment 3 extract group SGPT all significantly reduce (P＜0.01); Difference there are no significant meaning (P＞0.05) between fruit of Fructus Schisandrae Chinensis total lignans extract group, embodiment 1 extract group, the embodiment 3 extract groups.Illustrate that Fructus Schisandrae Chinensis carpopodium (fruit stem) and leaf total lignans extract are suitable to the therapeutical effect of carbon tetrachloride hepatic injury mice with fruit of Fructus Schisandrae Chinensis total lignans extract, all the SGPT that the carbon tetrachloride hepatic injury mice is caused raises significant inhibitory effect is arranged.
Claims (10)
1. extract the new raw material of Fructus Schisandrae Chinensis total lignanoid, it is characterized in that: the extract that the sub-carpopodium of five tastes of medicinal herb and Folium Schisandrae Chinensis are obtained through PROCESS FOR TREATMENT, its total lignans weight content is 30-95%, can substitute fruit of Fructus Schisandrae Chinensis total lignans extract and be used as medicine or go into health food.
2. the new raw material of Fructus Schisandrae Chinensis total lignanoid according to claim 1, it is characterized in that: Folium Schisandrae Chinensis and its plant origin of Fructus Schisandrae Chinensis carpopodium are one or both in the following plants: the plant of magnoliaceae schisandra Schisandra chinensis Bill. and schisandra chinensis Schisandrasphenanthera Rehd.et Wils. and other Schizandras.
3. the new raw material of extraction Fructus Schisandrae Chinensis total lignanoid according to claim 1 and 2 is characterized in that: described lignanoid is one or more in the following compounds, 1: schisandrin; 2: schisantherin B; 3: deoxyschizandrin; 4: schisandrin B; 5: schisandrin C; 6: pseudo-; 7: neoschisandrin; 8: isoschizandrin; 9: SALLRSIPA SAL ADNF-9 schizronol schisanhenol; 10: schisanhenol B; 11: schisanhenol acetate; 12: schizantherin A; 13: schizantherin B; 14: schizantherin C; 15: schizantherin D; 16: schizantherin E; 17: rubschisantherin; 18: Gomisin D.; 19: Gomisin E.; 20: Gomisin F.; 21: Gomisin G.; 22: Gomisin H.; 23: gomisin K
124: gomisin K
225: gomisin K
326: gomisin L
127: gomisin L
228: gomisin M
129: gomisin M
230: Ge Mixin N; 31: Ge Mixin 0; 32: Ge Mixin R; 33: Radix Angelicae Sinensis acyl Gomisin H.; 34: Radix Angelicae Sinensis acyl Ge Mixin 0; 35: Radix Angelicae Sinensis acyl Gomisin P.; 36: Radix Angelicae Sinensis acyl Ge Mixin Q; 37: along root of Dahurian angelica acyl Gomisin H.; 38: along root of Dahurian angelica acyl Gomisin P.; 39: the benzoyl Gomisin H..
4. the preparation technology of a Fructus Schisandrae Chinensis total lignanoid extract as claimed in claim 1 is characterized in that: (1) pulverizes decocting in water with Folium Schisandrae Chinensis, carpopodium drying; With residue drying behind the water extraction, a kind of heating and refluxing extraction of the methanol with 50～95%, ethanol, isopropyl alcohol or n-butyl alcohol, the extracting solution concentrating under reduced pressure is the concentrated solution of density 0.80-1.20 or the extractum that concentrated solution obtains behind organic solvent extraction, adopt the macroporous adsorptive resins enrichment, water, 40%～75% ethanol, 95% ethanol elution, collect 95% ethanol elution, the extract that obtains after concentrating, its total lignans weight content is 30%-95%;
(2), pulverize decocting in water with Folium Schisandrae Chinensis, carpopodium (fruit stem) drying; With residue drying behind the water extraction, a kind of heating and refluxing extraction of the methanol with 70～95%, ethanol, isopropyl alcohol or n-butyl alcohol, extracting solution is evaporated to dried, organic solvent room temperature or heating and refluxing extraction, extract adopts the chromatographic column eluting, the extract that obtains behind the eluting, its total lignans weight content is 30%-95%.
5. the preparation technology of Fructus Schisandrae Chinensis total lignanoid extract according to claim 4 is characterized in that: organic solvent is ether, alcohol, ester, the ketone of polarity less than n-butyl alcohol; The solvent elution mode comprises the positive of single or mixed solvent, anti-phase or gradient elution.
6. put forward the preparation technology of thing according to the described Fructus Schisandrae Chinensis total lignanoid of claim 4, it is characterized in that: described adsorbent resin is one or more in phenylethylene, phenylpropionic acid, third rare amide-type or the nitrogen oxide type macroporous resin.
7. the preparation technology of Fructus Schisandrae Chinensis total lignanoid extract according to claim 4 is characterized in that: chromatographic column filler is one or more in silica gel, aluminium oxide, polyamide, adsorbent resin, glucose gel, ODS, the kieselguhr.
8. the new raw material of extraction Fructus Schisandrae Chinensis total lignanoid according to claim 1 is characterized in that: the extract that is obtained by this raw material can be mixed and made into acceptable drug and food dosage form clinically with pharmaceutically acceptable excipient or carrier
9. the new raw material of extraction Fructus Schisandrae Chinensis total lignanoid according to claim 8 is characterized in that: described dosage form is tablet, capsule, powder, tincture, oral liquid, drop pill and injection or beverage, bread, cookies, confection, wine, tea and cosmetics etc.
10. extract the purposes of the new raw material of Fructus Schisandrae Chinensis total lignanoid, it is characterized in that: the extract that is obtained by this raw material is used for the preparation prevention or treatment hepatitis product is used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101591969A CN101214278B (en) | 2007-12-26 | 2007-12-26 | New raw material for extracting fructus schizandrae total lignans and preparation technique and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101591969A CN101214278B (en) | 2007-12-26 | 2007-12-26 | New raw material for extracting fructus schizandrae total lignans and preparation technique and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101214278A true CN101214278A (en) | 2008-07-09 |
| CN101214278B CN101214278B (en) | 2012-06-27 |
Family
ID=39620826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101591969A Expired - Fee Related CN101214278B (en) | 2007-12-26 | 2007-12-26 | New raw material for extracting fructus schizandrae total lignans and preparation technique and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101214278B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101786945A (en) * | 2009-12-28 | 2010-07-28 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching and purifying schizandrol A in schisandra |
| CN101978990A (en) * | 2010-09-30 | 2011-02-23 | 曹云峰 | Schisandra extract and preparation method and application thereof |
| CN102050707A (en) * | 2009-11-01 | 2011-05-11 | 桂林莱茵生物科技股份有限公司 | Method for extracting deoxyschisandrin and schisandrin B from schisandra |
| CN102302554A (en) * | 2011-09-15 | 2012-01-04 | 哈尔滨仁皇药业股份有限公司 | Extraction method of schisandra total lignans |
| CN101720964B (en) * | 2009-12-02 | 2012-05-23 | 长白朝鲜族自治县松云野生经济植物有限责任公司 | Health-care drink prepared from shizandra berries |
| CN102731271A (en) * | 2011-12-25 | 2012-10-17 | 大兴安岭林格贝有机食品有限责任公司 | New method for purifying schisandrin |
| CN103141833A (en) * | 2013-02-04 | 2013-06-12 | 北华大学 | Health food with anti-aging effect and preparation method thereof |
| CN103751184A (en) * | 2014-01-09 | 2014-04-30 | 广东药学院 | Application of pregnane X receptor antagonist in preparation of lipid regulation and liver protection product |
| CN103848809A (en) * | 2012-11-30 | 2014-06-11 | 沈阳药科大学 | Dibenzocyclooctene lignans, microbial conversion products thereof and applications of the microbial conversion products |
| CN105477153A (en) * | 2014-08-01 | 2016-04-13 | 卢比肯股份有限公司 | Adaptogenic compositions and method for production thereof |
| CN106177119A (en) * | 2016-07-18 | 2016-12-07 | 南京正宽医药科技有限公司 | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof |
| CN106266168A (en) * | 2016-08-30 | 2017-01-04 | 扬子江药业集团南京海陵药业有限公司 | A kind of preparation method of Fructus Schisandrae Sphenantherae lignanoid extract |
| CN107126460A (en) * | 2017-04-12 | 2017-09-05 | 西安医学院 | A kind of preparation method of fructus schizandrae total lignans |
| CN107468803A (en) * | 2017-07-27 | 2017-12-15 | 磐安县派普特生物科技有限公司 | The preparation method of shizandra active extract |
| CN107513008A (en) * | 2017-07-27 | 2017-12-26 | 浦江县美泽生物科技有限公司 | Shizandra active extract |
| US10159268B2 (en) | 2013-02-08 | 2018-12-25 | General Mills, Inc. | Reduced sodium food products |
| CN111875483A (en) * | 2020-08-25 | 2020-11-03 | 长春工业大学 | Method for extracting lignans compounds from schisandra chinensis |
| WO2022143252A1 (en) * | 2020-12-29 | 2022-07-07 | 云南生物谷药业股份有限公司 | Preparation method for pharmaceutical composition |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100363318C (en) * | 2005-02-08 | 2008-01-23 | 朱萧俊 | Extraction of schizandrol A |
-
2007
- 2007-12-26 CN CN2007101591969A patent/CN101214278B/en not_active Expired - Fee Related
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050707A (en) * | 2009-11-01 | 2011-05-11 | 桂林莱茵生物科技股份有限公司 | Method for extracting deoxyschisandrin and schisandrin B from schisandra |
| CN101720964B (en) * | 2009-12-02 | 2012-05-23 | 长白朝鲜族自治县松云野生经济植物有限责任公司 | Health-care drink prepared from shizandra berries |
| CN101786945A (en) * | 2009-12-28 | 2010-07-28 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching and purifying schizandrol A in schisandra |
| CN101786945B (en) * | 2009-12-28 | 2014-01-01 | 大兴安岭科丽尔生物工程有限责任公司 | Method for enriching and purifying schizandrol A in schisandra |
| CN101978990A (en) * | 2010-09-30 | 2011-02-23 | 曹云峰 | Schisandra extract and preparation method and application thereof |
| CN101978990B (en) * | 2010-09-30 | 2013-06-05 | 曹云峰 | Schisandra extract and preparation method and application thereof |
| CN102302554B (en) * | 2011-09-15 | 2013-08-07 | 哈尔滨仁皇药业股份有限公司 | Extraction method of schisandra total lignans |
| CN102302554A (en) * | 2011-09-15 | 2012-01-04 | 哈尔滨仁皇药业股份有限公司 | Extraction method of schisandra total lignans |
| CN102731271B (en) * | 2011-12-25 | 2014-11-19 | 大兴安岭林格贝有机食品有限责任公司 | New method for purifying schisandrin |
| CN102731271A (en) * | 2011-12-25 | 2012-10-17 | 大兴安岭林格贝有机食品有限责任公司 | New method for purifying schisandrin |
| CN103848809A (en) * | 2012-11-30 | 2014-06-11 | 沈阳药科大学 | Dibenzocyclooctene lignans, microbial conversion products thereof and applications of the microbial conversion products |
| CN103141833A (en) * | 2013-02-04 | 2013-06-12 | 北华大学 | Health food with anti-aging effect and preparation method thereof |
| US11540539B2 (en) | 2013-02-08 | 2023-01-03 | General Mills, Inc. | Reduced sodium food products |
| US10159268B2 (en) | 2013-02-08 | 2018-12-25 | General Mills, Inc. | Reduced sodium food products |
| CN103751184A (en) * | 2014-01-09 | 2014-04-30 | 广东药学院 | Application of pregnane X receptor antagonist in preparation of lipid regulation and liver protection product |
| CN105477153B (en) * | 2014-08-01 | 2019-11-29 | 卢比肯股份有限公司 | Adapt to former composition and its production method |
| CN105477153A (en) * | 2014-08-01 | 2016-04-13 | 卢比肯股份有限公司 | Adaptogenic compositions and method for production thereof |
| CN106177119A (en) * | 2016-07-18 | 2016-12-07 | 南京正宽医药科技有限公司 | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof |
| CN106266168A (en) * | 2016-08-30 | 2017-01-04 | 扬子江药业集团南京海陵药业有限公司 | A kind of preparation method of Fructus Schisandrae Sphenantherae lignanoid extract |
| CN107126460A (en) * | 2017-04-12 | 2017-09-05 | 西安医学院 | A kind of preparation method of fructus schizandrae total lignans |
| CN107468803A (en) * | 2017-07-27 | 2017-12-15 | 磐安县派普特生物科技有限公司 | The preparation method of shizandra active extract |
| CN107513008A (en) * | 2017-07-27 | 2017-12-26 | 浦江县美泽生物科技有限公司 | Shizandra active extract |
| CN111875483A (en) * | 2020-08-25 | 2020-11-03 | 长春工业大学 | Method for extracting lignans compounds from schisandra chinensis |
| CN111875483B (en) * | 2020-08-25 | 2022-11-29 | 长春工业大学 | Method for extracting lignanoid compounds from schisandra chinensis |
| WO2022143252A1 (en) * | 2020-12-29 | 2022-07-07 | 云南生物谷药业股份有限公司 | Preparation method for pharmaceutical composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101214278B (en) | 2012-06-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101214278B (en) | New raw material for extracting fructus schizandrae total lignans and preparation technique and use | |
| Chen et al. | Cytotoxicity and antihyperglycemic effect of minor constituents from Rhizoma Coptis in HepG2 cells | |
| US10758585B2 (en) | Beautyberry total glycosides extract and preparation method and use thereof | |
| CN101181317B (en) | Purslane extract as well as preparation method and application thereof | |
| CN101721488B (en) | Pharmaceutical composition for treating liver diseases and prepration method thereof | |
| CN101554409A (en) | Long pepper alkaloid and preparation method, preparation and application thereof | |
| CN101700289B (en) | Traditional Chinese medicine extract composition for treating gastrointestinal dysfunction or irritable bowel syndrome (IBS), and preparation method and applications thereof in preparation of medicaments | |
| Li et al. | Fructus arctii: an overview on its traditional uses, pharmacology and phytochemistry | |
| US8628806B2 (en) | Hypolipidemic composition and its use | |
| CN101933973B (en) | Medicament composition for preventing and treating liver damage | |
| CN1327859C (en) | Schisandra fruit extractive, its preparation process and purposes | |
| CN109674848A (en) | A kind of preparation method and purposes of licorice | |
| CN107266464B (en) | A kind of rhizoma alismatis decoction extract and its application | |
| CN103006781B (en) | Compound Dai medicine extract with liver-protecting effect and preparation method thereof | |
| CN102309543B (en) | Compound salvia miltiorrhiza concentrated preparation and preparation and detection method thereof | |
| CN104435293B (en) | Application of the Kadsura heteroclita total triterpene ethanol extract in preparation anti-arthritic drugs | |
| CN103860818A (en) | Traditional Chinese medicine composition and application thereof | |
| CN102397481A (en) | Medicinal preparation for wind dispelling and heat dissipating and preparation method thereof | |
| CN101269123A (en) | Secondary development novel technique for thirst eliminating capsule for lowering blood sugar | |
| CN1302792C (en) | Medicine composition with the action of relieving alcoholism and protecting liver and its application | |
| CN114848764B (en) | Traditional Chinese medicine compound composition for preventing and treating liver injury and preparation method and application thereof | |
| CN103301256A (en) | Preparation method of medicinal plant extract being capable of reducing blood fat and application thereof in pharmacy and health food | |
| CN103784623A (en) | Preparation method of medicinal plant extract for reducing blood sugar and application in pharmacy and health food thereof | |
| CN113041308B (en) | A Chinese medicinal composition with anti-inflammatory and toxic materials clearing away effects, and its preparation method | |
| CN102204964B (en) | Composite natural medicament used for preventing and treating metabolic syndrome and preparation method and medicinal application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120627 Termination date: 20171226 |