CN1302792C - Medicine composition with the action of relieving alcoholism and protecting liver and its application - Google Patents
Medicine composition with the action of relieving alcoholism and protecting liver and its application Download PDFInfo
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Abstract
The present invention relates to a medicine composition with the functions for relieving alcoholism and protecting the liver, which is prepared from cape jasmine fruit, pawpaw, schisandra fruit, red sage root and grape pip as raw materials and is processed into a product which has the functions for relieving alcoholism and protecting the liver. The medicine composition has the effects for reducing fever for detoxification, strengthening the stomach, eliminating dampness and regulating qi to dissipate blood stasis so as to relieve the toxicity of alcohol. Experimental researches prove that the medicine composition has the functions for improving the alcohol tolerance ability of human bodies, reducing headaches caused by alcohol, headaches, nausea, vomitus and entogastric uncomfortableness, lowering liver damage caused by alcohol and treating fatty liver. The medicine composition can be prepared into medicines or health care foods with the functions for relieving alcoholism and protecting the liver, other kinds of foods and health care products.
Description
The present invention relates to that a kind of tool relieves the effect of alcohol, the pharmaceutical composition of hepatoprotective effect, compositions is made up of Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae, Semen Vitis viniferae, is folk remedy originally, the history of using surplus in the of existing 50 year as the side of relieving the effect of alcohol.And obtained splendid effect through animal experiment study and human body application.
Wine is a kind of special beverage, since wine has been invented the Shang dynasty by China, wine and spirits culture just are accompanied by the course that people passed by thousands of years, wine also is present in us at one's side as a kind of alternative media simultaneously as a kind of beverage, drink existing certain effect of invigorating blood circulation on a small quantity, again certain excitation can be arranged, can increase the atmosphere of social lives such as red-letter day and party, but wine also is very large to the harm of human body.Though people have also recognized the harm of wine, because its toxic action to liver has certain disguise, so people pay little attention to its harm.In fact, the tolerance of wine is varied with each individual, but ethanol lethasl concentration and indifference in the blood be generally 87-152mmol/L, so liver are most important to the removing ability of ethanol.
The main absorption approach of wine is a digestive tract, absorbed ethanol major part enters liver from portal vein, 90-98% wherein is at liver metabolism, only the ethanol of 2%-10% is got rid of through urine, sweat gland and respiratory tract, the metabolism main path (80%) of ethanol in liver is for to be decomposed into acetaldehyde by ethanol dehydrogenase (ADH), change S-acetyl-coenzyme-A by aldehyde dehydrogenase into as coenzyme again, further change carbon dioxide and water into through tricarboxylic acid cycle, or by other chemical compound of citric acid circulation changing into.Have approximately 20% by microsome oxidation of ethanol enzyme system (MEOS) metabolism, small part is by nadph oxidase (DPNH I), the metabolism of hydrogen peroxide enzyme system.Often drink and also can make NADH and H in the hepatocyte
+Increase, promoted to be oxidized to the response system of NAD, lactic acid is increased by NADH, bring out gout (Wang Tailing, Zhao Jingbo. the pathology of alcoholic liver disease. gastroenterology and hepatopathy magazine, 1997,6:4-8).
Drink for a long time and can hinder decomposition of fatty acids, cause that neutral fat accumulates, because superfluous H
+Can hinder the interior fatty acid of liver cell mitochondria and decompose, cause fatty liver as the energy.Hepatocellular fatization has had a strong impact on the normal function of liver, has suppressed the carrying out of tricarboxylic acid cycle in the body, causes the metabolic pathway of wine to be obstructed, and co-solvents forms vicious cycle to the infringement of normal structure.
Because wine also is simultaneously an important industry of country as a kind of traditional history in existing thousand of beverage application, total ban is drunk infeasible substantially.Therefore study and a kind ofly can reduce ethanol toxicity, and can have the medicine of restitution that bigger social benefit and application prospect are arranged hepatic injury due to the ethanol.
Chinese medicine thinks that wine is damp and hot poisonous property, and the pathogenesis key of " getting sick from drinking too much wine " or " alcoholophilia " is heat (poison) impairing the liver, qi depression to blood stasis, wet resistance taste.The principle of control mainly be heat-clearing and toxic substances removing, the blood stasis dispelling of stomach function regulating dampness dissipating, QI regulating (He Fujin, Ben Changen etc. Herba Sidae Rhombifoliae soup is to the protective action of rat alcoholic liver injury, combination of Chinese and Western medicine hepatopathy magazine, 1994,4 (3): 19-21).
The object of the present invention is to provide can improve, headache dizzy after drinking, feel sick with treatment, the pharmaceutical composition of vomiting, gastric malaise symptoms and alcoholic liver injury, fatty liver.Said composition has clearing away heat-damp and promoting diuresis, dissolving blood stasis and detoxication, and the liver stomach is also controlled, and row is held back mutually and held concurrently, and brings into play the health-care effect of relieving alcoholism and protecting the liver jointly from too many levels.
Fructus Gardeniae has the pathogenic fire purging relieving restlessness in the compositions, heat clearing away resolving depression, the merit of eliminating damp, detoxifying.Main effective ingredient is the iridoid glycoside compounds, wherein content than higher be gardenoside.Fructus Gardeniae extract can significantly reduce serum bilirubin level, ALT and AST activity, and hepatocyte is had protective effect.(cloudy strong, the Guo Li bow. Chinese medicine modern study and clinical practice II. Beijing: Xueyuan Press, front page .1993,473).
The removing dampness of Fructus Chaenomelis stomach function regulating, suppressing the hyperactive liver is dredged muscle, and energy alcoholic intoxication (" book on Chinese herbal medicine unit life bud "), emesis rush down convulsion.Can prevent swelling of liver cell, alleviate fatty degeneration of liver, and promote hepatocyte to repair, remarkable reduction ALT level (editorial board of State Administration of Traditional Chinese Medicine's " China's book on Chinese herbal medicine ". China herbal (selected), Shanghai: Shanghai science tech publishing house, front page .1998,761).
The Radix Salviae Miltiorrhizae blood circulation promoting and blood stasis dispelling contains phenolic acid and Diterpenes composition, and representation compound is with salvianolic acid B and tanshinone.The energy microcirculation improvement; remove free radical; suppress the hepatocyte lipid peroxidation; promote hepatocellular regeneration and reparation; the energy anti-hepatic fibrosis; to alcoholic liver injury have significant protective effect (Lai Lingyan. Radix Salviae Miltiorrhizae is to the preliminary study of experimental alcoholic liver injury protective effect, Guangxi Medical University's journal, 16 (4): 480-481).
The Fructus Schisandrae Chinensis convergence is astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid.Can alcoholic intoxication (" solar corona ").Contain multiple lignanoid, representation compound is a schisandrin B.Can obviously induce the cytochrome P-450 activity of hepatomicrosome, strengthen the function of detoxification of liver.Also have anti-hepatocellular damage, alleviate liver lipid peroxidation damage due to the ethylism pharmacological action (institute of Materia Medica,Chinese Academy of Medical Sciences. Chinese herbal medicine modern study, Beijing: Beijing Medical University, combined publication society of China Concord Medical Science University, front page .1995, the first volume, 372-375).
Anthocyanidin is Semen Vitis viniferae extract, contains procyanidin compounds.Have very high radical scavenging activity, can suppress the lipid peroxidation of erythrocyte membrane and low density lipoprotein, LDL.Can also promote the formation of endotheliocyte relaxation factor NO, the damage of pair cell and tissue shield country (Gao Juntao, etc. polyphenols is to the ESR of oxygen radical removing effect research, Wave Spectrum magazine, 1999,16 (5): 409-414) in the Semen Vitis viniferae.
This shows, compositions can play by comprehensive approach relieve the effect of alcohol, hepatoprotective effect.This pharmaceutical composition is made by the raw material of following weight ratio:
Fructus Gardeniae 12-25 Fructus Chaenomelis 2-10 Fructus Schisandrae Chinensis 3-10
Radix Salviae Miltiorrhizae 3-10 Semen Vitis viniferae 1-6
Weight ratio is preferably:
Fructus Gardeniae 12-20 Fructus Chaenomelis 2-8 Fructus Schisandrae Chinensis 4-8
Radix Salviae Miltiorrhizae 5-8 Semen Vitis viniferae 3-5
Preferred weight ratio is:
Fructus Gardeniae 16 Fructus Chaenomeliss 4 Fructus Schisandrae Chinensis 5
Radix Salviae Miltiorrhizae 6 Semen Vitis viniferae 4
Medicine can be pulverized the back by said medicine and directly use, also can replace by extract, the extract consumption is: the Fructus Gardeniae extract consumption is the 1/12-1/8 of Fructus Gardeniae medical material, the Fructus Chaenomelis extract consumption is the 1/4-1/2 of Fructus Chaenomelis medical material, the Fructus Schisandrae Chinensis extrat consumption is the 1/8-1/4 of schisandra chinensis medicinal material, the Radix Salviae Miltiorrhizae extract consumption is the 1/8-1/4 of red rooted salvia, and the Semen Vitis viniferae extract consumption is the 1/5-1/2 of Semen Vitis viniferae.
Above-mentioned each medical material also can all replace with extract, and the composition weight ratio of extract is:
Fructus Gardeniae extract 10-30 Fructus Chaenomelis extract 10-30 Fructus Schisandrae Chinensis extrat 8-20
Radix Salviae Miltiorrhizae extract 8-20 Semen Vitis viniferae extract 1-6
Composition weight ratio through preferred extract is:
Fructus Gardeniae extract 16 Fructus Chaenomelis extract 12 Fructus Schisandrae Chinensis extrats 10
Radix Salviae Miltiorrhizae extract 10 Semen Vitis viniferae extracts 4
Except that the above-mentioned method of extracting respectively, Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae can also merge processing, Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae can be handled with decocting-second alcohol deposition method (concentration of alcohol is 60-85%) respectively, alcohol liquid reclaims and obtains, or after extracting with methods such as infusion process, percolation, circumfluence method with 60-85% ethanol, reclaim pure liquid and obtain extract, and can be further refining through solvent processing, extraction or column chromatography method etc. on this basis.Or after Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae merge in proportion, decocting-second alcohol deposition method (concentration of alcohol is 60-85%) is handled, alcohol liquid reclaims and obtains, or after extracting with infusion process, percolation, circumfluence method with 60-85% ethanol, reclaim pure liquid and obtain extract, and can be further refining through solvent processing, extraction or column chromatography method etc. on this basis.Wherein, main effective ingredient is iridoids such as gardenoside in the extract of Fructus Gardeniae, the main effective ingredient of Fructus Schisandrae Chinensis extrat is Lignanoids compounds such as deoxyschizandrin, second element, and the main effective ingredient of Radix Salviae Miltiorrhizae extract is diterpene-kind compounds such as phenolic acid compounds such as salvianolic acid B or tanshinone.The consumption of extract is equivalent to the 1/10-1/4 of Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, red rooted salvia, with the amount ratio of Semen Vitis viniferae extract be united extraction thing 40-100, Semen Vitis viniferae extract 1-4.
Semen Vitis viniferae can extract with following method, extracts with infusion process, percolation with 40-60% ethanol, and temperature is no more than 70 ℃ during recovery.Or extract with infusion process, percolation with 40-60% acetone, temperature is no more than 60 ℃ during recovery.Or by carbon dioxide supercritical fluid extraction, and can be further refining through solvent processing, extraction or column chromatography method etc. on this basis.Main effective ingredient is a procyanidin compounds in the extract.
Also can directly use procyanidin compounds in the compositions to replace Semen Vitis viniferae or Semen Vitis viniferae extract, consumption is equivalent to the 1/15-1/8 of Semen Vitis viniferae consumption, or is equivalent to the 1/7-1/2 of Semen Vitis viniferae extract consumption.
The product that this pharmaceutical composition is made can suitably add necessary adjuvant, excipient, additive in preparation process.It can be the acceptable any dosage form of pharmaceutics, also can be the acceptable any type of product of bromatology, as oral liquid, and peroral dosage form and injection types such as tablet, capsule, electuary, granule, pill, powder, teabag, beverage class.
By the product of method for preparing, can be medicine, health food, other classification food, health product.The purposes of this product is that it can be used to increase human body to the ethanol tolerance, improves the headache, the dizziness that cause after drinking with treatment, feels sick, symptom such as vomiting gastric discomfort, improves and the treatment alcoholic liver injury treatment fatty liver.
The tablet that this pharmaceutical composition is made " Hu Dan " (seeing embodiment 9), be used for zoopery, find to take obviously to improve the liver function of the animal of taking ethanol for a long time, reduce the deposition degree of peroxyester matter in liver, obviously reduce the drunk rate of animal, improve animal ethanol tolerance.Be used for human body, can obviously improve easily headache patient's the headache symptom of back of drinking, effective percentage is 90.6%.
Be noted that infringement that ethanol causes liver by light to heavily being a progressive process, by liver function preliminary impaired to alcoholic fatty liver, spill smart hepatitis, alcoholic fibrosis and alcoholic cirrhosis and also finally cause death.Be the irreversibility pathological changes when hardening, existing medical condition and level can't be cured.Therefore, should be to the treatment of alcoholic liver injury to cut off source of damage (do not drink or drink less) and to treat targetedly simultaneously and be advisable.Taking medicine when drinking can only alleviate damage and can not avoid damage fully.
Embodiment 1: the preparation of capsule
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae, Semen Vitis viniferae, cross 120 mesh sieves behind the pulverize separately, mix homogeneously (weight ratio is 25: 10: 10: 10: 6) incapsulates, promptly.
Embodiment 2: the preparation of powder
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae, Semen Vitis viniferae, cross 120 mesh sieves behind the pulverize separately, mix homogeneously (weight ratio is 12: 2: 4: 5: 3), promptly.
Embodiment 3: the preparation of teabag
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae, Semen Vitis viniferae, cross 40 mesh sieves after the coarse pulverization respectively, mix homogeneously (weight ratio is 16: 4: 5: 6: 4), pack is promptly.
Embodiment 4: the preparation of pill
Getting Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae handles with decocting-alcohol deposition method respectively.At first decocting is three times, and each 40 minutes, water consumption was respectively 8,5,5 times of material quantity, and water liquid merges, be concentrated into 1.5 times of volumes of material quantity after the filtration, add 95% ethanol liquid, to containing alcohol 60%, alcohol liquid reclaims, and pulverizes behind the gained extract dry, crosses 120 mesh sieves.Other gets Semen Vitis viniferae, and 20 mesh sieves are crossed in coarse pulverization, divides 5 dippings to extract with 30 times of amount 60% ethanol, and each 24 hours, extract filtered the back and reclaims solvent, and temperature is 70 ℃, and gained extractum vacuum drying was pulverized 120 mesh sieves.Each extract powder mix homogeneously (weight ratio is a Fructus Gardeniae extract 10: Fructus Chaenomelis extract 10: Fructus Schisandrae Chinensis extrat 8: Radix Salviae Miltiorrhizae extract 8: Semen Vitis viniferae extract 1), and with the Mel mixing pill of new refining, promptly.
Embodiment 5: the preparation of electuary
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae and mix that (weight ratio is 12: 2: 3: 3), handle with decocting-alcohol deposition method.At first decocting is three times, and is each 40 ', and water consumption is respectively 8,5,5 times of raw material consumption, and water liquid is concentrated into 1.5 times of volumes after filtering, and adds 95% ethanol liquid, and to containing alcohol 85%, pure liquid reclaims, and pulverizes behind the gained extract dry, crosses 120 mesh sieves.Other gets Semen Vitis viniferae, 20 mesh sieves are crossed in coarse pulverization, extract with 30 times of amount 40% acetone percolation, extract filters back reclaim under reduced pressure acetone, and temperature is 60 ℃, water liquid suitably concentrates the back and goes up macroporous resin column, the water-ethanol gradient elution is collected the 30-60% part, and 60 ℃ of vacuum decompressions reclaim solvent, gained extractum vacuum drying was pulverized 120 mesh sieves.(weight ratio is a mixed extract 40 to two extract powder mix homogeneously: Semen Vitis viniferae extract 1), with the Mel mixed pelletization of new refining, be pressed into the square shape or directly with granule encapsulation, promptly.
Embodiment 6: the preparation of granule
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae and use 70% alcohol reflux each 30 ' respectively, be respectively 8,5,5 times of raw material consumption, filter the back and concentrate, pulverize behind the gained extract dry, cross 120 mesh sieves with the alcohol amount.Other gets Semen Vitis viniferae, and coarse pulverization is crossed 20 mesh sieves, CO
2Supercritical extraction, the extractum aqueous dispersion divides 6 extractions with 10 times of amount ethyl acetate, after the water backwash, the acetic acid ethyl acetate extract decompression and solvent recovery, gained extractum vacuum drying was pulverized 120 mesh sieves.Each extract powder mix homogeneously (weight ratio is a Fructus Gardeniae extract 30: Fructus Chaenomelis extract 30: Fructus Schisandrae Chinensis extrat 20: Radix Salviae Miltiorrhizae extract 20: Semen Vitis viniferae extract 6) moistening a little with 40% ethanol, granulate promptly.
Embodiment 7: the preparation of beverage
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae and extract three times with 75% alcohol dipping respectively, each 24 hours, measure 10,10,10 times that are respectively material quantity with alcohol, filter the back and concentrate, pulverize behind the gained extract dry, cross 120 mesh sieves.Other gets Semen Vitis viniferae, and 20 mesh sieves are crossed in coarse pulverization, extract with 30 times of amount 40% acetone percolation, extract filters back reclaim under reduced pressure acetone, and temperature is 60 ℃, and water liquid suitably concentrates the back and goes up macroporous resin column, the water-ethanol gradient elution, collect the 30-60% part, 60 ℃ of solvent recovered under vacuum, gained extractum vacuum drying, pulverize, cross 120 mesh sieves.(weight ratio is a Fructus Gardeniae extract 16: Fructus Chaenomelis extract 12: Fructus Schisandrae Chinensis extrat 10: Radix Salviae Miltiorrhizae extract 10: Semen Vitis viniferae extract 4) be major ingredient, major ingredient accounts for 8% to each extract powder mix homogeneously during the system beverage, adds sucrose 5% seasoning, uses distilled water, promptly.
Embodiment 8: the preparation of oral liquid
Get Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae and extract three times with 65% alcohol dipping respectively, each 24 hours, measure 10,10,10 times that are respectively material quantity with alcohol, filter the back and concentrate, pulverize behind the gained extract dry, cross 120 mesh sieves.Other buys commercially available procyanidin (in catechin procyanidin content＞95%), crosses 120 mesh sieves.(weight ratio is a Fructus Gardeniae extract 16 to each extract powder mix homogeneously: Fructus Chaenomelis extract 12: Fructus Schisandrae Chinensis extrat 10: Radix Salviae Miltiorrhizae extract 10: procyanidin 1), add 5 times of water gagings, promptly.
Embodiment 9: the preparation of tablet
Getting Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae handles through decocting-precipitate with ethanol (75%) respectively, behind the filtration drying, pulverize, cross 120 mesh sieves, (weight ratio is a Fructus Gardeniae extract 16 to commercially available procyanidin mixing: Fructus Chaenomelis extract 12: Fructus Schisandrae Chinensis extrat 10: Radix Salviae Miltiorrhizae extract 10: procyanidin 0.4), the adjuvant ratio is 37.5%, makes tablet (the heavy 0.75g/ grain of sheet), promptly.
Embodiment 9 prepared tablets (claiming " Hu Dan " again) are carried out pharmacodynamic experiment, to its relieve the effect of alcohol, hepatoprotective effect studies, the results are shown in hereinafter experimental example, zoopery used " Hu Dan " tabletting for mixing according to the above ratio but not in the following experimental example with adjuvant, human " Hu Dan " is a tablet, the heavy 0.75g/ grain of sheet.
Experimental example 1: the drunk prevention experiment of mice
Experiment material: " Hu Dan " high, medium and low dosage group, get " Hu Dan " and, be mixed with debita spissitudo with water dissolution.Commercially available Erguotou wine (56 °, cattle pen mountain, Beijing brewery produces, the aqueous solution that is mixed with spirituosity 40% is standby).
Method: get 40 of Kunming kind white mice, the male and female dual-purpose, body weight 18-20g is divided into 4 groups at random, every group 10, be set at model group respectively, high, medium and low dosage group, each is organized and irritates stomach every day and give wine 0.4ml/ only, and model group is given 30min before the wine every day, irritates stomach feedwater 0.4ml; Each administration group gives wine preceding 30min every day, equal gastric infusion 0.4ml, carried out continuously 30, and in the time of the 2nd, 5,10,15,20 day, mice is carried out the righting reflex experiment respectively, the number of elements of righting reflex loss occurs observe for behind the ethanol mice in 1 hour, righting reflex loss can think that animal is drunk, 5 observed results of accumulative total, its drunk rate.
The result: experimental result shows that each administration group and model group compare, and drunk rate obviously reduces, and shows that it has the drunk effect of good preventing.
The drunk prevention experiment of table 1 mice
| Group | Dosage (g/kg) | Number of animals | Drunk (inferior) number | Drunk rate (%) |
| Dosage group low dose group model group in the high dose group | 1.28 0.43 0.21 / | 10 10 10 10 | 2 5 10 37 | 4 10 20 74 |
Embodiment 2: to the influence of peroxy esters matter (LPO) in the liver function of the rat of the hepatic injury due to the ethanol and the liver
Getting SD is 50 of rats, and body weight is 200-250g, is divided into 5 groups at random, i.e. normal control group, model control group, " Hu Dan " high, medium and low dosage group.Feedwater normal control group every day, and be beverage with the tap water, be feedstuff with pure corn, all the other each groups are beverage with 40 degree wine, feedstuff is that corn adds Adeps Sus domestica (20%) and cholesterol (0.5%).Three administration group administration every day 0.4ml/ only.Each group was raised 30 days with said method, got hematometry liver function and LPO value, and the results are shown in Table 2 in the 30th day.
Table 2 " Hu Dan " is to the influence of alcohol damaged Mouse Liver function and peroxyester matter (x ± s)
| Group | Dosage (g/kg) | Number of animals | ALT (glutamate pyruvate transaminase) | AST (glutamic oxaloacetic transaminase, GOT) | AKP (acid phosphatase) | LPO (peroxyester matter) |
| Dosage group low dose group model control group Normal group in the high dose group | 1.28 0.43 0.21 / / | 10 10 10 10 10 | 46.75±12.52 *** 53.14±1319 ** 62.84±10.35 72.25±12.33 △△△ 30.32±9.27 | 180.31± 37.45 *** 208.62± 38.21 *** 258.73± 44.32 * 277.92±36.36 △△△ 123.11±20.63 | 14.38± 7.46 *** 20.38± 6.73 *** 24.89±7.75 30.26±8.35 △△△ 12.15±5.59 | 2.102± 0.973 *** 4.494± 0.822 *** 5.327± 0.950 *** 8.263± 0.871△△△ 0.742± 0.304 |
△ △ △ compares P＜0.001 with the normal control group; * *, * *, * compare P＜0.001, P＜0.01, P＜0.05 with model control group
The result shows; compare with the normal control group; the every biochemical indicator of model control group animal liver function all has obvious change; this liver injury model success is described; each administration group is compared with the model group animal; every biochemical indicator has largely and to reduce, and illustrate that the administration group has significant protective effect to the hepatic injury of formation, but to compare every index still higher with the normal control group.
Normal control group LPO value is lower, shows its interior free yl removing ability than higher, and liver lactone matter peroxide is in reduced levels.Compare with the normal control group, LPO content obviously raises in the liver homogenate of model control group animal, and each administration group LPO that compares with model group all has in various degree reduction.But compare with the normal group animal, the LPO value still has rising in various degree.Illustrate that the long-term ethanol of taking in can cause the liver damage of mice, hepatocyte ester materialization degree increases, but uses " Hu Dan " when taking in ethanol, and hepatocyte ester materialization degree is obviously reduced.
Embodiment 3: analgesic activity
The writhing method analgesic experiment
Laboratory animal; 40 of Kunming mouses, male and female half and half, body weight 18-20g is divided into 4 groups at random.Be respectively " Hu Dan " high, medium and low dosage group and normal control group, gastric infusion, the feedwater of normal control group.Successive administration five days, 0.4ml/ are only.After the last administration 0.5 hour, every Mus lumbar injection 0.6% acetic acid 0.1ml/kg, the writhing response that mice produces behind the observation injection algogen, each Mus is turned round the body number of times in back 20 minutes of the record injection, and relatively each group difference the results are shown in Table 3.
Table 3 is turned round body analgesic experiment result
| Group | Dosage (g/kg) | Number of animals (only) | Turn round body number of times (inferior) |
| High dose group | 1.28 | 10 | 18.56±16.38 ** |
| Middle dosage group | 0.43 | 10 | 25.89±13.15 * |
| Low dose group | 0.21 | 10 | 26.27±13.42 |
| Normal control | / | 10 | 41.50±18.94 |
Annotate: compare with the normal control group,
*P＜0.01;
*P＜0.05
By analgesic experiment as seen, compare with the normal control group, each administration group all has in various degree raising to the tolerance of pain, and high, middle dosage group is compared significant difference with matched group.
Embodiment 4: to the effect of ethanol headache
Case is selected: select easily headache (frequency of drinking height, on average weekly more than the secondary, nearest five all headaches of drinking) person 85 people in the crowd that drinks for a long time, age 25-60 year, be all the male.
Therapeutic Method: respectively take two " Hu Dan " half an hour before and after drinking, drinking amount therewith before during headache the amount of drinking suitable.
Curative effect determinate standard:
Produce effects: no headache symptom
Effectively: the headache degree alleviates, and the headache time shortens.
Invalid: headache as before.
The result: after taking a Hu Dan, 72 people that have of the transference cure of having a headache, other has 5 people to reflect that the headache degree alleviates, and the headache time shortens.Calculate thus, obvious effective rate is 84.7%, and effective percentage is 90.6%.
Claims (22)
1 one kinds of pharmaceutical compositions with effects of relieving alcoholism and protecting liver is characterized in that this pharmaceutical composition made by the raw material of following weight ratio:
The Fructus Gardeniae 12-25 Fructus Chaenomelis 2-10 3-10 that distinguishes the flavor of
Radix Salviae Miltiorrhizae 3-10 Semen Vitis viniferae 1-6.
The described pharmaceutical composition of 2 claim 1 is characterized in that this pharmaceutical composition made by the raw material of following weight ratio:
Fructus Gardeniae 12-20 Fructus Chaenomelis 2-8 Fructus Schisandrae Chinensis 4-8
Radix Salviae Miltiorrhizae 5-8 Semen Vitis viniferae 3-5.
The described pharmaceutical composition of 3 claim 1 is characterized in that this pharmaceutical composition made by the raw material of following weight ratio:
Fructus Gardeniae 16 Fructus Chaenomeliss 4 Fructus Schisandrae Chinensis 5
Radix Salviae Miltiorrhizae 6 Semen Vitis viniferae 4.
4 pharmaceutical compositions according to claim 1 is characterized in that every kind of raw material is all replaced by its extract, and the composition weight ratio of extract is:
Fructus Gardeniae extract 10-30 Fructus Chaenomelis extract 10-30 flavor seed extract 8-20
Radix Salviae Miltiorrhizae extract 8-20 Semen Vitis viniferae extract 1-6.
5 pharmaceutical compositions according to claim 1 is characterized in that every kind of raw material is all replaced by its extract, and the composition weight ratio of extract is:
Fructus Gardeniae extract 16 Fructus Chaenomelis extract 12 flavor seed extracts 10
Radix Salviae Miltiorrhizae extract 10 Semen Vitis viniferae extracts 4.
6 pharmaceutical compositions according to claim 1, it is characterized in that every kind of raw material is all replaced by its extract, the extract consumption is: the Fructus Gardeniae extract consumption is equivalent to the 1/12-1/8 of Fructus Gardeniae weight, the Fructus Chaenomelis extract consumption is equivalent to the 1/4-1/2 of Fructus Chaenomelis weight, the Fructus Schisandrae Chinensis extrat consumption is equivalent to the 1/8-1/4 of Fructus Schisandrae Chinensis weight, the Radix Salviae Miltiorrhizae extract consumption is equivalent to the 1/8-1/4 of Radix Salviae Miltiorrhizae weight, and the Semen Vitis viniferae extract consumption is equivalent to the 1/5-1/2 of Semen Vitis viniferae weight.
The described pharmaceutical composition of 7 claim 1, it is characterized in that every kind of raw material is all replaced by its extract, wherein Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae united extraction are obtained extract, the consumption of this extract is equivalent to the 1/10-1/4 of raw material Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae gross weight, with the amount ratio of Semen Vitis viniferae extract be mixed material extract 40-100, Semen Vitis viniferae extract 1-4.
The described pharmaceutical composition of 8 claim 4, the extract that it is characterized in that Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae prepares according to following method: Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae are handled with decocting-second alcohol deposition method respectively, wherein concentration of alcohol is 60-85%, alcohol liquid reclaims and obtains, reclaim pure liquid and obtain extract, and further refining through solvent processing, extraction or column chromatography method on this basis.
The described pharmaceutical composition of 9 claim 7, the extract that it is characterized in that Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae prepares according to following method: after Fructus Gardeniae, Fructus Chaenomelis, Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae are merged in proportion, decocting-ethanol precipitation is handled, wherein concentration of alcohol is 60-85%, alcohol liquid reclaims and obtains, reclaim pure liquid and obtain extract, and further refining through solvent processing, extraction or column chromatography method on this basis.
The described pharmaceutical composition of 10 claim 8, the ethanol that it is characterized in that decocting-ethanol precipitation usefulness 60-85% is with infusion process or percolation or circumfluence method extraction replacement.
The described pharmaceutical composition of 11 claim 4, the extract that it is characterized in that Semen Vitis viniferae prepares according to following method, Semen Vitis viniferae is extracted with infusion process, percolation with 40-60% ethanol, temperature is no more than 70 ℃ during recovery, and further refining through solvent processing, extraction or column chromatography method on this basis.
The described pharmaceutical composition of 12 claim 4, the extract that it is characterized in that Semen Vitis viniferae prepares according to following method, Semen Vitis viniferae is extracted with infusion process, percolation with 40-60% acetone, temperature is no more than 60 ℃ during recovery, and further refining through solvent processing, extraction or column chromatography method on this basis.
The described pharmaceutical composition of 13 claim 4 is characterized in that the extract of Semen Vitis viniferae prepares according to following method, with the Semen Vitis viniferae carbon dioxide supercritical fluid extraction, and further refining through solvent processing, extraction or column chromatography method on this basis.
The described pharmaceutical composition of 14 claim 4 is characterized in that Semen Vitis viniferae extract replaces with commercially available procyanidin compounds.
The described pharmaceutical composition of 15 claim 1 is characterized in that Semen Vitis viniferae replaces with commercially available procyanidin compounds, uses in compositions, and consumption is equivalent to the 1/15-1/8 of Semen Vitis viniferae consumption.
The described pharmaceutical composition of 16 claim 1 is characterized in that adding adjuvant, excipient, additive in preparation process.
The described pharmaceutical composition of 17 claim 1 is characterized in that any type of product that food prepared therefrom is accepted.
The described pharmaceutical composition of 18 claim 17 is characterized in that being prepared into following dosage form: oral liquid, the product of tablet, capsule, electuary, granule, pill, powder, teabag, beverage class peroral dosage form.
The described pharmaceutical composition of 19 claim 1 has the purposes aspect the medicine, health food, other classification food, health product of effects of relieving alcoholism and protecting liver in preparation.
The purposes of the described pharmaceutical composition of 20 claim 1 is characterized in that its antialcoholism action is meant that it has the effect of increase human body to the ethanol tolerance.
The purposes of the described pharmaceutical composition of 21 claim 20, it is characterized in that its antialcoholism action be meant its have improve the headache, the dizziness that cause after drinking with treatment, feel sick, the effect of vomiting, gastric malaise symptoms.
The described pharmaceutical composition of 22 claim 20 is characterized in that effects of relieving alcoholism and protecting liver, has the effect that improves with the treatment alcoholic liver injury.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021040370A CN1302792C (en) | 2002-03-07 | 2002-03-07 | Medicine composition with the action of relieving alcoholism and protecting liver and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB021040370A CN1302792C (en) | 2002-03-07 | 2002-03-07 | Medicine composition with the action of relieving alcoholism and protecting liver and its application |
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| CN1443543A CN1443543A (en) | 2003-09-24 |
| CN1302792C true CN1302792C (en) | 2007-03-07 |
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| CNB021040370A Expired - Fee Related CN1302792C (en) | 2002-03-07 | 2002-03-07 | Medicine composition with the action of relieving alcoholism and protecting liver and its application |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102784248B (en) * | 2012-09-03 | 2014-07-02 | 赵全成 | Chinese medicine composition for preventing and treating alcoholic liver injury |
| CN103550238A (en) * | 2013-10-26 | 2014-02-05 | 苏州天南星生物科技有限公司 | Composition containing procyanidine |
| CN103519186A (en) * | 2013-11-02 | 2014-01-22 | 苏州天南星生物科技有限公司 | Health food for auxiliary protection against chemical liver injury |
| CN107890105A (en) * | 2017-10-18 | 2018-04-10 | 天津大学 | Application of the papaya extractives in Medicament for Alcoholism is prepared |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1229674A (en) * | 1999-02-09 | 1999-09-29 | 郑力国 | Tonic Chinese medicine for preventing harm of alcohol to human body, and its process method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1229674A (en) * | 1999-02-09 | 1999-09-29 | 郑力国 | Tonic Chinese medicine for preventing harm of alcohol to human body, and its process method |
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