CN103301256A - Preparation method of medicinal plant extract being capable of reducing blood fat and application thereof in pharmacy and health food - Google Patents
Preparation method of medicinal plant extract being capable of reducing blood fat and application thereof in pharmacy and health food Download PDFInfo
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- CN103301256A CN103301256A CN2012100587914A CN201210058791A CN103301256A CN 103301256 A CN103301256 A CN 103301256A CN 2012100587914 A CN2012100587914 A CN 2012100587914A CN 201210058791 A CN201210058791 A CN 201210058791A CN 103301256 A CN103301256 A CN 103301256A
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Abstract
The invention relate to a preparation method of a medicinal plant extract being capable of reducing blood fat and an application thereof in pharmacy and health food. The extract is prepared by using a medicinal plant-Pandanus tectorius in south of the Five Ridges as a raw material, extracting and separating. The preparation method comprises extracting the Pandanus tectorius by water and/or alcohol, and then condensing and drying the extract to obtain the medicinal plant extract provided by the invention. The extract is dissolved in water, degreased, and extracted by an organic solvent to obtain an extraction part; or passes through macroporous adsorption resin, and then elute by water, methanol, ethanol, acetone or 60-90 % ethanol or aqueous acetone; the extract liquor or the eluent is collected, recovered under reduced pressure, condensed into extract, and then dried to obtain effective parts of the medicinal plant extract. A plurality of pharmacodynamics experiments verify that the medicinal plant extract is capable of reducing the blood fat, can be used for preparing drugs or health products of reducing the blood fat, and thus is used for treating hyperlipidemia, obesity and other diseases.
Description
Technical field
The present invention relates to Chinese traditional medicines depressing lipid and field of health care food, specifically is the medicinal plants-wild pineapple extract of a kind of blood fat reducing, fat-reducing and preparation method and the application in pharmacy and health product exploitation of effective site thereof.
Background technology
Hyperlipemia is one of main inducing of cardiovascular and cerebrovascular disease generation, is the latency that threatens human health.At present, using fat-reducing medicament is the most favourable measure of hyperlipemia disease prevention and treatment.At present commercially available compound preparation or Chinese patent medicine have Colestid, Xuezhikang, grant health, DIAOXINXUE KANG etc.The research and development of blood fat reducing Chinese medicine and ethnic drug are also in the ascendant always, as effect for reducing blood fat research of Fructus Crataegi, Herb Gynostemmae Pentaphylli, Radix Ginseng, Radix Et Rhizoma Rhei, Radix Notoginseng, Semen Cassiae, Fructus Lycii etc.In these research process, find many have remarkable hypolipidemic activity such as saponins, flavonoid, polysaccharide etc., simultaneously the blood fat reducing mechanism of these effective ingredient has also been carried out a large amount of research, comprise the absorption that suppresses cholesterol, suppress the effect that the factors such as HMG-CoA enzyme, increased SOD, NO reach blood fat reducing, these all are that scientific basic has been established in the research and development of follow-up blood fat reducing medicinal plants or ethnic drug undoubtedly.
Wild Fructus Ananadis comosi is the fruit of Radix Pandani Furcati section (Pandanaceae) Pandanus (Pandanus) plant Pandanus tectorius (L.) Parkins. (Pandanus tectorius Soland.).Wild Fructus Ananadis comosi grows in the torrid zone or subtropical zone, is distributed in ground such as Guangdong, Guangxi, Yunnan, Hainan, Taiwan in China, and resource is very abundant, records in " Guangdong Province's Chinese crude drug standard ", " Hainan Province's Chinese crude drug standard ", " Nanning City's medicine will ".Wild its form of Fructus Ananadis comosi is peculiar, hard and contain wooden, but document is put down in writing its tonifying the spleen and stomach, reinforcing premodial qi, alcoholic intoxication, cures mainly liver-heat asthenic fire, cirrhotic ascites etc.
Beneficial effect of the present invention and meaning are: the extract among the present invention and effective site are from the fruit extraction of Pandanus south of the Five Ridges medicinal plants Pandanus tectorius (L.) Parkins. (Landaus tectorius Soland.), purification and get, this plant resources is very abundant, we discover active drug or the health product that can be used as preparation blood fat reducing, fat-reducing, for this medicinal plants of further comprehensive utilization, drive south of the Five Ridges medical material expanding economy and have very important practical sense simultaneously.
Summary of the invention
The invention provides always preparation and the application in exploitation fat-reducing medicament and health product thereof with medicinal plant extract.
Fat-reducing medicament of the present invention or health product preparation method of extract may further comprise the steps:
(1), extract:
Get wild Fructus Ananadis comosi, clean, break into pieces with one's fingers, join 10～20 times water or/and soaked overnight, decoction, filtration in the alcohol; Medicinal residues after the filtration add water again or/and alcohol extraction 1-3 time, filter, merging filtrate, then filtrate decompression are concentrated, and are heated to 60 ℃, and it is the concentrated solution of 1.10-1.30 that heat is surveyed relative density, and concentrated solution is chilled to room temperature.
(2), purification:
With the medicinal liquid after concentrating, by crude drug weight: water volume ratio is 0.5-3: 1 ratio adds neutral water, is heated to 30-50 ℃, and stirring makes dissolving, leaves standstill 2-24 hour, filters to get filtrate;
With gained filtrate after organic solvent degreasing is handled, with organic solvent extraction or extraction, gained extract or extracting solution are through concentrating, dry, namely get effective site, or with absorption with macroporous adsorbent resin on the resulting extracting solution, first washing decontamination with 3～5 bed volumes, carry out eluting with eluting solvent then, till no obvious speckle is known in the thin layer chromatography inspection; Collect eluent, drying namely gets effective site;
Described organic solvent for defat is petroleum ether, benzene, chloroform, ethyl acetate, cyclohexane extraction, normal hexane.
Used extraction or extraction are acetone, ethanol, methanol, n-butyl alcohol, propanol, isopropyl alcohol, ethyl acetate, water, ethanol-water solution, methanol-water solution with solvent.
Described eluting solvent is the ethanol of water, methanol, ethanol, acetone or 50-95v% or the aqueous solution of acetone.
The macroporous resin that adopts comprises D series, HPD series, HP series, XAD series and AB-8 macroporous adsorbent resin.
The preparation method of medicinal plant extract of the present invention and effective site comprises medicinal plant extract of the present invention or effective site is mixed with pharmaceutically suitable carrier.
Medicinal plant extract of the present invention can be made into various dosage forms, for example powder, tablet, pill, dispersant, capsule, soft capsule, drop pill, granule, effervescent tablet, tea bag, oral liquid, health beverage etc.
Technology contents disclosed according to the present invention, those skilled in the art will very clear other embodiments of the present invention, and following embodiment is only made example.Under the situation of not violating purport of the present invention and scope, can carry out various changes and improvements to the present invention.These changes and improvements all should be within protection scope of the present invention.
Description of drawings
Fig. 1: wild pineapple fruit water extract effect for reducing blood fat result
Fig. 2: the blood fat reducing function of medicinal plants effective site of the present invention
The specific embodiment
Below in conjunction with case study on implementation the present invention is described in further detail.
Medicinal plant extract among embodiment 1 the present invention and effective site preparation example () thereof
Get the wild Fructus Ananadis comosi of fresh maturation, place container, add 10-20 times of water, soak 2h after, decocts 3 times, each 2h, filtration is concentrated into dried medicinal plant extract.Medicinal plant extract is behind water dissolution, separate through the D-101 macroporous adsorbent resin, be eluted to colourlessly with Different concentrations of alcohol-aqueous solution, eluent makes water elution thing, 70% ethanol elution thing through concentrating under reduced pressure, concentrate, namely get the effective site among the present invention after the spray drying.
Medicinal plant extract among embodiment 2 the present invention and effective site preparation example (two) thereof
Get the wild Fructus Ananadis comosi after fresh maturation is dried in the shade, place container, add 10-20 times of ethanol or alcohol-water (7: 3), soak 2h after, cocurrent flow extracts 3 times, each 2h filters, and is concentrated into dried medicinal plant extract.Medicinal plant extract after organic solvent chloroform or ethyl acetate defat, is extracted to colourlessly behind water dissolution repeatedly with n-butyl alcohol, extract namely gets the effective site among the present invention after the spray drying behind concentrating under reduced pressure.
Test material: wild Fructus Ananadis comosi water extract, fat arteries and veins health, high lipid food, test kit (T-CHOL)
Experimental animal: healthy Kunming mouse (18～20g)
Test method: get 60 of 18～22g male mice in kunming, (dosage is respectively 25g/kg to be divided into normal group, high blood lipid model group and 5 dosage groups of wild Fructus Ananadis comosi water extract at random, 50g/kg, 100g/kg), 4.0mg/kg simvastatin group, totally 9 groups of 20g/kg fat arteries and veins health groups (10/group), except the normal group conventional feed is fed, other organize equal feeding high lipid food, irritate stomach simultaneously and give relative medicine, and normal group and high blood lipid model group are irritated stomach equal-volume tap water.Behind the administration 18d, each is organized the mice fasting and can't help water 12h, plucks eyeball and gets blood, and the centrifugal 10min of 3000r/min gets serum, presses determined by ultraviolet spectrophotometry serum TC content with test kit.
Experimental result: wild pineapple fruit water extract hypolipidemic activity is studied with the hyperlipidemia mouse model, with simvastatin and the positive contrast medicine of fat arteries and veins health, found that its water extract is significantly active, especially it is significantly active to reduce T-CHOL in the model mice blood plasma (TC), reduction total plasma cholesterol activity when low concentration 25g/Kg is very remarkable (p＜0.01), and effect obviously is better than Chinese medicine fat arteries and veins health.
N＝10，vs?control，###P＜0.01；vs?model*P＜0.05，**P＜0.01.
The blood fat reducing activity test (Golden Hamster hyperlipemia model) of embodiment 4 medicinal plants effective sites of the present invention:
Test material: effective site, berberine, high lipid food, test kit comprise T-CHOL (TC), total triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein, LDL (LDL-C).
Laboratory animal: healthy Golden Hamster (130-160g)
Test method: get 60 of 130～160g Golden Hamster, be divided into 1 dosage group of wild Fructus Ananadis comosi water extract at random, high, normal, basic 3 the dosage groups of effective site, 1 group of positive drug, model group, totally 7 groups of blank groups (8/group), except the normal group conventional feed is fed, other organize equal feeding high lipid food (2% cholesterol, 15% Adeps Sus domestica, 0.4% cholate, 82.6% normal feedstuff), irritate stomach simultaneously and give relative medicine, the oral equal-volume tap water of normal group and high blood lipid model group.Behind the administration 21d, each is organized the Golden Hamster fasting and can't help water 12h, plucks eyeball and gets blood, and the centrifugal 10min of 3000r/min gets serum, presses determined by ultraviolet spectrophotometry serum TC, TG, LDL-C, HDL-C content with test kit.
N＝8，vs?control，###P＜0.01；vs?model?*P＜0.05，**P＜0.01，***P＜0.001.
Annotate: berberine group dosage is 200mg/Kg, water extract 400mg/Kg, effective site high dose 400mg/kg, dosage group 200mg/Kg in the effective site, effective site low dose group 100mg/Kg.
Medicinal plant extract among embodiment 5 the present invention or effective site powder preparation example
The prepared medicinal plant extract of embodiment or effective site and effective site are ground into fine powder, sieve, get 1 part of this medicinal plant extract fine powder of having sieved, add 6.5 parts of 3.5 parts of starch, lactose by the equivalent mixing method mix homogeneously that progressively increases, the method divided dose makes powder by measure.
The preparation example of embodiment 6 medicinal plant extracts of the present invention or effective site hard capsule
With embodiment 1 or 2 prepared medicinal plant extracts or effective site pulverizing, cross the 60-100 mesh sieve, get 1 part of this medicinal plant extract fine powder of having sieved, add 1.1 parts of dried starch as filler, add the 1-3% Pulvis Talci and make fluidizer, make hard capsule with the Capsules filling behind the mixing.
Medicinal plant extract among embodiment 7 the present invention or the preparation example of effective site tablet
Embodiment 1 or 2 prepared medicinal plant extracts or effective site were pulverized the 60-100 mesh sieve, get 1 part of this medicinal plant extract fine powder of having sieved, add hydroxypropyl cellulose and cook disintegrating agent for 1.1 parts, add medicinal dextrin and make binding agent for 0.4 part, the ethanol that adds an amount of 50-95% is made lubricant, the mix lubricant that adds 1-3% behind wet granulation, drying, the granulate is even, is pressed into tablet.
Medicinal plant extract among embodiment 8 the present invention or the preparation example of effective site dispersible tablet
4 parts of embodiment 1 or 2 prepared medicinal plant extracts or 1 part of effective site and carrier materials are dissolved in jointly in the ethanol of 50-95%, after the spray drying method drying, sieve, the microcrystalline Cellulose that adds 2.0 parts is cooked disintegrating agent, add an amount of micropowder silica gel and make fluidizer, behind the mix homogeneously powder directly is pressed into tablet, namely gets dispersible tablet.
Medicinal plant extract among embodiment 9 the present invention or the preparation example of effective site health beverage
With embodiment 1 or 2 prepared medicinal plant extracts or effective site is a and sugar-free sweetener is dissolved in the food grade water for 0.001 part jointly, the adding flavoring agent is prepared into the beverage of various tastes, namely gets health beverage after the fill.
Claims (14)
1. the medicinal plant extract of a blood fat reducing and effective site thereof, it is characterized in that: with wild Fructus Ananadis comosi (Pandanus tectorius (L.) Parkins. fruit) be that raw material is through extracting, concentrate and get, to be medicinal plant extract get through organic solvent extraction or extraction or purification with macroreticular resin effective site.
2. according to the preparation method of the medicinal plant extract described in the claim 1 and effective site thereof, it is characterized in that this method contain following 1., 2., 3. three steps:
1.: get wild Fructus Ananadis comosi, add 10～30 times of water gagings or/and alcohol extraction 2～4 times, each 1～3 hour, merge extractive liquid, filtered, and behind this filtrate concentrate drying of concentrating under reduced pressure, namely gets the medicinal plant extract among the present invention;
2.: effective site extraction process I: get 1. extract, soluble in water, obtain certain density aqueous solution, add in the macroporous adsorptive resins loaded, treat that this step finishes after, use the distilled water flushing resin column, colourless to eluent; In resin column, add eluant then, collect eluent, concentrate and dry, final effective site in must this present invention.
3.: effective site extraction process II: the extract in getting 1., soluble in water, obtain certain density aqueous solution, after the ungrease treatment, add solvent and extract or extract, behind extract or the extracting solution concentrate drying, obtain the effective site among the present invention.
3. according to the preparation method of claim 2 described in 1., it is characterized in that: used extraction solvent is that water is or/and alcohol.
4. according to the preparation method of claim 2 described in 1., it is characterized in that: adopt to decoct or reflux, extract,, extraction time is 2～4 times, and each time is 1～3 hour.
According to claim 2 2. described in preparation method, it is characterized in that: used chromatographic column filler is for comprising D series, HPD series, HP series, XAD series and AB-8 macroporous adsorbent resin.
According to claim 2 2. described in preparation method, it is characterized in that: used eluant is the ethanol of water, methanol, ethanol, acetone or 50-95v% or the aqueous solution of acetone.
7. according to the degreasing method of claim 2 described in 3., it is characterized in that: the defat organic solvent is petroleum ether, benzene, chloroform, ethyl acetate, cyclohexane extraction, normal hexane, gasoline etc.
8. according to extraction or the extracting method of claim 2 described in 3., it is characterized in that: extraction or to extract organic solvent be acetone, ethanol, methanol, n-butyl alcohol, propanol, isopropyl alcohol, ethyl acetate, water, ethanol-water solution, methanol-water, acetone-water solution.
9. medicinal plant extract according to claim 1 and effective site thereof, it is characterized in that: this medicinal plant extract and effective site thereof have effect for reducing blood fat.
10. the described a kind of medicinal plant extract of claim 1 and effective site thereof the application in the preparation blood lipid-lowering medicine.
11. the described a kind of medicinal plant extract of claim 1 and effective site thereof the application in preparation blood fat reducing, slimming health product.
12. according to the medicinal plant extract described in the claim 1 and effective parts formulation thereof, it is characterized in that: also comprise conventional pharmaceutic adjuvant.
13. lowering blood-fat and reducing weight medicine according to claim 10 is characterized in that: comprise powder, tablet, pill, dispersant, capsule, soft capsule, drop pill, granule, effervescent tablet, tea bag, oral liquid, health beverage etc.
14. the health product of lowering blood-fat and reducing weight according to Claim 8 is characterized in that: comprise beverage, capsule, effervescent tablet, tea bag, oral liquid, granule etc.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109043270A (en) * | 2018-08-06 | 2018-12-21 | 林健 | Natural fruit and vegetables component extracting method, solid beverage mix and beverage |
| CN111265600A (en) * | 2020-03-18 | 2020-06-12 | 海南医学院 | Application of jackfruit extract in preparing weight-reducing preparation |
-
2012
- 2012-03-08 CN CN2012100587914A patent/CN103301256A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 武嫱: "露兜簕果中有效成分的研究", 《中国优秀硕士学位论文全文数据库，医药卫生科技辑》 * |
| 许瑞波: "角鲨烯的制备及应用进展", 《山东医药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109043270A (en) * | 2018-08-06 | 2018-12-21 | 林健 | Natural fruit and vegetables component extracting method, solid beverage mix and beverage |
| CN111265600A (en) * | 2020-03-18 | 2020-06-12 | 海南医学院 | Application of jackfruit extract in preparing weight-reducing preparation |
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Application publication date: 20130918 |