CN108295131B - A kind of plant extract composition with antihypertensive effect, its preparation method and application - Google Patents

A kind of plant extract composition with antihypertensive effect, its preparation method and application Download PDF

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CN108295131B
CN108295131B CN201810282367.5A CN201810282367A CN108295131B CN 108295131 B CN108295131 B CN 108295131B CN 201810282367 A CN201810282367 A CN 201810282367A CN 108295131 B CN108295131 B CN 108295131B
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qianluo
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CN108295131A (en
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公衍玲
金宏
马畅
孙润州
孔佳
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Cadbury Pharmaceutical Biotechnology Shenzhen Co ltd
Chengdu Yishenrui Technology Co ltd
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Qingdao University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/638—Ligustrum, e.g. Chinese privet
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

本发明涉及一种具有降压作用的植物提取物组合物、其制备方法及应用。该植物提取物组合物由芊罗核提取物、山楂核提取物和泽漆提取物按7‑12:3‑5:1‑4的比例组成，该组合物可降低动脉舒张压和/或收缩压，用于制备降压药物。该组合物所采用的原料价廉易得，成本较低，制备方法简单，各组合物之间发挥协同增效作用，疗效确切，安全可靠，具有良好的降血压功效，便于应用和推广。The present invention relates to a plant extract composition with antihypertensive effect, its preparation method and application. The plant extract composition is composed of spirulina kernel extract, hawthorn kernel extract and lacquer lacquer extract in a ratio of 7-12:3-5:1-4, and the composition can reduce arterial diastolic blood pressure and/or constriction pressure for the preparation of antihypertensive drugs. The raw materials used in the composition are cheap and easy to obtain, the cost is low, the preparation method is simple, the compositions play a synergistic effect, the curative effect is definite, safe and reliable, has a good blood pressure lowering effect, and is convenient for application and popularization.

Description

Plant extract composition with blood pressure reducing effect, preparation method and application thereof
Technical Field
The invention relates to a plant extract composition, in particular to a plant extract composition consisting of a Qian Luo nuclear extract, a hawthorn seed extract and a euphorbia helioscopia extract, a preparation method of the plant extract composition and application of the plant extract composition in preparation of antihypertensive drugs.
Background
Hypertension refers to the increase of systolic pressure and/or diastolic pressure of arteries in a resting state, is a chronic disease mainly manifested by the continuous increase of arterial blood pressure, often causes the pathological changes of important organs such as heart, brain, kidney and the like and has corresponding consequences, often accompanies the metabolic disturbance of fat and sugar, and long-term hypertension can cause cardiovascular diseases such as myocardial infarction, coronary heart disease and the like, and can also cause renal arteriosclerosis, thereby causing renal insufficiency, uremia and the like.
Hypertension is the most common cardiovascular disease, is a major public health problem worldwide, and is also the fuse of various diseases, which can increase the risk of coronary heart disease, heart failure and kidney disease. At present, the common treatment means is to use antihypertensive drugs, such as captopril tablets, compound antihypertensive tablets and the like, which have certain curative effect although being convenient to take, but the curative effect is not easy to consolidate, once the drugs are stopped, the recurrence rate is high, and some drugs have great damage to the functions of the liver and the kidney after being taken for a long time. Various medicines are successively researched and developed in the field of traditional Chinese medicine to reduce blood pressure, but the treatment effect is not obvious, the treatment course is long, and the treatment effect is unstable.
For a long time, the traditional Chinese medicine is concerned by people because of definite curative effect, little side effect and difficult relapse. Qianluo is a plant of Ligustrum genus of Oleaceae family, and is mainly distributed along the coast of Shandong peninsula. Qian Luo Xiguang is cold-resistant and mostly grows at the high cliff stone slit with the elevation of 600 + 1000 m along the sea. Qian Luo Zhi, Ye, Guo and Gen all can be used as medicine, and its leaves are nutritious and can be drunk as tea. At present, Qianluo leaves are reported to contain a plurality of amino acids, 10 inorganic elements such as Ca, K, Mg, Na and the like, and also contain rich medicinal components such as triterpene acid, chlorogenic acid, flavonoid compounds and the like, and have pharmacological effects of clearing away heat and toxic materials, inhibiting bacteria and diminishing inflammation, inhibiting and preventing cancers, regulating mechanisms, resisting radiation, resisting aging, reducing cholesterol and triglyceride, calming nerves and benefiting sleep and the like, and nutritional health-care effects. However, the development and utilization of Qianluo nucleus have not been found yet. The semen crataegi is seed of Crataegus pinnatifida Bunge var. major N.E.Br., Crataegus cuneata Sieb.et Zucc. or Crataegus scabrifolia (Franch.) Rehd. etc. of Crataegus of Rosaceae. Researches show that the main components of the hawthorn seeds comprise flavonoid components such as hyperin, quercetin, rutin and the like, are rich in linolenic acid, linoleic acid, triterpenic acid, fumaric acid, citric acid and a large amount of vitamin C, and have multiple functions of stimulating appetite, helping digestion, reducing blood pressure, reducing blood fat, improving immunity and the like. Herba Euphorbiae Helioscopiae is the overground part of Euphorbia Helioscopica L. belonging to Euphorbiaceae, is a crude drug distributed in all provinces of China except Xinjiang and Tibet, and contains multiple bioactive components such as herba Euphorbiae Helioscopiae saponin, succinic acid, sterol and various vitamins.
The present inventors have finally obtained the optimal pharmaceutical ratio composition of the present invention through a great deal of research and experiments on the ratio of each formulation, and have demonstrated its therapeutic effect on hypertension, thereby completing the present invention.
Disclosure of Invention
The invention aims to provide a plant extract composition which has the advantages of cheap and easily obtained raw materials, definite curative effect, safety, reliability, convenient application and popularization and good blood pressure reducing effect.
The second object of the present invention is to provide a method for preparing the above plant extract composition.
The third purpose of the invention is to provide the application of the plant extract composition in preparing the antihypertensive drug.
The invention provides a plant extract composition, which consists of a Qian Luo nuclear extract, a hawthorn seed extract and a euphorbia helioscopia extract according to the proportion of 7-12:3-5: 1-4.
The plant extract composition comprises semen Qian Luo extract, semen crataegi extract and herba Euphorbiae Helioscopiae extract at a ratio of 10:4: 3.
The semen Qian Luo extract, semen crataegi extract and herba Euphorbiae Helioscopiae extract can be obtained by conventional purification method known by those skilled in the art, and can also obtain the same therapeutic effect. The methods listed below are only the methods that the inventors consider to be more preferable and are not intended to make particular restrictions on the methods of extraction of the components of the plant composition.
Preferably, the preparation method of the extract of the Qianluo nucleus in the plant extract composition comprises the following steps:
pulverizing semen Qianluo, sieving, placing in round bottom flask, adding 8-10 times of 70% ethanol, heating and reflux extracting in electric heating jacket for 2 times (2 hr and 1 hr for each time), filtering, mixing filtrates, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain semen Qianluo extract.
The preparation method of the hawthorn seed extract in the plant extract composition comprises the following steps:
taking hawthorn seeds, crushing, sieving, soaking for 24h, filtering, placing in a round-bottom flask, adding 16 times of water, adjusting the pH to 5.0 with hydrochloric acid, adding 0.8% cellulase, placing in a water bath kettle, performing enzymolysis at a constant temperature of 40 ℃ for 30min, transferring the round-bottom flask into an ultrasonic oscillator for extraction for 2 times, 1.5h each time, wherein the ultrasonic temperature is 80 ℃ and the ultrasonic frequency is 100KHz, filtering, combining filtrates, concentrating, drying under reduced pressure to obtain a dry extract, crushing, and sieving with a 80-mesh sieve to obtain the hawthorn seed extract.
The preparation method of herba Euphorbiae Helioscopiae extract in the above plant extract composition comprises:
drying herba Euphorbiae Helioscopiae, pulverizing, sieving, and treating with supercritical CO2Extracting with entrainer ethanol at solid-to-liquid ratio of 1.5ml/mg, extraction pressure of 20MPa, extraction temperature of 45 deg.C, extraction time of 2h, filtering the extractive solution, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain herba Euphorbiae Helioscopiae extract.
The invention also provides a preferable preparation method of the plant extract composition, which comprises the following steps:
(1) preparation of Qianluo nuclear extract
Pulverizing semen Qianluo, sieving, placing in round bottom flask, adding 8-10 times of 70% ethanol, heating and reflux extracting in electric heating jacket for 2 times (2 hr and 1 hr for each time), filtering, mixing filtrates, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain semen Qianluo extract.
(2) Preparation of semen crataegi extract
Taking hawthorn seeds, crushing, sieving, soaking for 24h, filtering, placing in a round-bottom flask, adding 16 times of water, adjusting the pH to 5.0 with hydrochloric acid, adding 0.8% cellulase, placing in a water bath kettle, performing enzymolysis at a constant temperature of 40 ℃ for 30min, transferring the round-bottom flask into an ultrasonic oscillator for extraction for 2 times, 1.5h each time, wherein the ultrasonic temperature is 80 ℃ and the ultrasonic frequency is 100KHz, filtering, combining filtrates, concentrating, drying under reduced pressure to obtain a dry extract, crushing, and sieving with a 80-mesh sieve to obtain the hawthorn seed extract.
(3) Preparation of herba Euphorbiae Helioscopiae extract
Drying herba Euphorbiae Helioscopiae, pulverizing, sieving, and treating with supercritical CO2Extracting with entrainer ethanol at solid-to-liquid ratio of 1.5ml/mg, extraction pressure of 20MPa, extraction temperature of 45 deg.C, extraction time of 2h, filtering the extractive solution, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain herba Euphorbiae Helioscopiae extract.
(4) Selecting semen Qian Luo extract, semen crataegi extract and herba Euphorbiae Helioscopiae extract at a certain proportion, and mixing.
The plant extract composition for preparing the antihypertensive drug is prepared into a preparation containing the plant extract composition with the specification of 200mg or 400mg by adopting the conventional preparation technology, and the selection of the dosage form is not particularly limited, so that the plant extract composition can be prepared into tablets, capsules, granules and the like.
The invention also comprises the application of the plant extract composition in preparing antihypertensive drugs.
The beneficial effects of the plant extract composition on the aspect of reducing blood pressure are as follows:
1. the plant extract composition is derived from natural plants, is low in price and easy to obtain, has a simple process, can effectively enrich the antihypertensive active ingredients, has a reliable and stable antihypertensive effect, does not rebound, does not have toxic or side effect, and is suitable for long-term administration of patients.
2. The inventor originally combines the Qian Luo nuclear extract, the hawthorn seed extract and the euphorbia helioscopia extract, and determines the dosage proportion among the components through a large number of experiments, so that the composition has obvious synergistic effect and obtains good antihypertensive effect.
Detailed Description
The technical solution of the present invention is further described with reference to the following specific examples.
Example 1: the invention relates to a plant extract composition and preparation of a preparation thereof
The first step is as follows: preparation of various extracts
(1) The preparation method of the Qianluo nuclear extract comprises the following steps:
pulverizing semen Qianluo, sieving, placing in round bottom flask, adding 8-10 times of 70% ethanol, heating and reflux extracting in electric heating jacket for 2 times (2 hr and 1 hr for each time), filtering, mixing filtrates, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain semen Qianluo extract.
(2) The preparation method of the hawthorn seed extract comprises the following steps:
taking hawthorn seeds, crushing, sieving, soaking for 24h, filtering, placing in a round-bottom flask, adding 16 times of water, adjusting the pH to 5.0 with hydrochloric acid, adding 0.8% cellulase, placing in a water bath kettle, performing enzymolysis at a constant temperature of 40 ℃ for 30min, transferring the round-bottom flask into an ultrasonic oscillator for extraction for 2 times, 1.5h each time, wherein the ultrasonic temperature is 80 ℃ and the ultrasonic frequency is 100KHz, filtering, combining filtrates, concentrating, drying under reduced pressure to obtain a dry extract, crushing, and sieving with a 80-mesh sieve to obtain the hawthorn seed extract.
(3) The preparation method of the euphorbia helioscopia extract comprises the following steps:
drying herba Euphorbiae Helioscopiae, pulverizing, sieving, performing supercritical extraction with entrainer ethanol at solid-to-liquid ratio of 1.5ml/mg, extraction pressure of 20MPa, extraction temperature of 45 deg.C, extraction time of 2h, filtering the extractive solution, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain herba Euphorbiae Helioscopiae extract.
The second step is that: preparation of the formulations
(1) Preparation of the natural medicine composition capsule of the invention
Respectively weighing 120mg of Qian Luo kernel extract, 45mg of hawthorn kernel extract and 35mg of herba Euphorbiae Helioscopiae extract prepared in the above steps, simultaneously weighing 155mg of starch and 45mg of dextrin, mixing uniformly, filling into capsules, and preparing into capsules with each pure weight of 400 mg.
(2) Preparation of the Natural pharmaceutical composition tablet of the invention
Respectively weighing 120mg of Qian Luo nuclear extract, 45mg of hawthorn seed extract and 35mg of herba Euphorbiae Helioscopiae extract prepared in the above steps, simultaneously weighing 150mg of starch and 45mg of dextrin, mixing uniformly, spraying 80% ethanol for wet granulation, drying, granulating, adding 5mg of magnesium stearate, mixing uniformly, tabletting, and making into tablets with a pure weight of 400 mg.
(3) Preparation of the natural pharmaceutical composition granule of the invention
Respectively weighing the above prepared semen Qian Luo extract 120mg, semen crataegi extract 45mg, herba Euphorbiae Helioscopiae extract 35mg, simultaneously weighing starch 145mg, dextrin 115mg, and sugar powder 25mg, mixing well, spraying 65% ethanol for wet granulation, drying, and granulating to obtain each bag with pure weight
400mg of granules.
The blood pressure lowering effect of the composition with the blood pressure lowering effect of the present invention is further illustrated by the following test examples.
Test example 1: the plant extract composition of the invention has the function of reducing blood pressure of Spontaneous Hypertension (SHR) rats
1. Test materials
Qianluo nucleus, which is prepared from mountain Laoshan Qianluo fruit of Qingdao, by cleaning, removing pulp, air drying, and identifying as seed of Qianluo of Oleaceae;
semen crataegi, from Shandong Mengyin county food canned food factory, identified as seed of Crataegus pinnatifida of Crataegus of Rosaceae;
herba Euphorbiae Helioscopiae, purchased from Okinawa Zhongsheng pharmacy of Shandong province, and identified as whole plant of Euphorbia Helioscopia of Euphorbiaceae;
preparing the composition of the semen Qian Luo extract and the hawthorn seed extract (composition 1), the composition of the semen Qian Luo extract and the herba Euphorbiae Helioscopiae extract (composition 2), the composition of the semen crataegi extract and the herba Euphorbiae Helioscopiae extract (composition 3) and the composition of the plant extract of the invention (composition 4) according to the above embodiments, respectively, and preparing into 100mg/ml solution when in use;
gastrodia elata-eucommia ulmoides capsule, Yunnanxin Biotechnology Co., Ltd, is poured out when in use, and is prepared into 200mg/ml solution by using normal saline;
10 clean SD male rats and 60 SHR male rats are all 10-12 weeks old and provided by Qingdao Daren Fucheng livestock Limited company, and are used for experiments after being fed for 5-7 days in an adaptive manner.
2. Test method
10 SD rats served as blank control group, 60 SHR rats were randomly divided into 6 groups, each of which was 10, and the groups were respectively model group, composition 1 group, composition 2 group, composition 3 group, composition 4 group and Gastrodia elata-eucommia ulmoides capsule group. The composition groups were respectively infused with the corresponding composition (0.2ml/100g body weight), the blank control group and the model group were administered with the same amount of normal saline, and the rhizoma Gastrodiae and Eucommiae cortex capsule group was administered with the capsule (0.2ml/100g body weight). It is administered 1 time daily for 4 weeks. In each group, the tail arterial blood of the rat in the waking state is measured by a monitor for animals, and the blood pressure is measured once a week.
3. Test results
The results are shown in Table 1. As can be seen from Table 1, after each group of drugs had been infused, there was a different degree of reduction in blood pressure in rats from week 2 (P <0.05-0.01 compared to the model group) compared to the model group. The composition 4 has obvious blood pressure reducing effect, and the blood pressure of rats is reduced to normal by 4 th week (compared with a normal control group, P is more than 0.05; compared with a model group, P is less than 0.01), so that the composition is obviously superior to other compositions and has the same effect as the gastrodia elata and eucommia ulmoides capsules.
TABLE 1 Effect of the compositions on the blood pressure in SHR rats
Figure BDA0001614996740000041
Figure BDA0001614996740000042
Note: in comparison with the blank set, the results,ΔP<0.05，ΔΔP<0.01; in comparison with the set of models,*P<0.05，**P<0.01; in comparison with the composition 1, the composition,#P<0.05，##P<0.05; in comparison with the composition 2, the present inventors have shown that,▲P<0.05，▲▲P<0.01; in comparison with the composition 3, the composition,$P<0.05，$$P<0.01。

Claims (1)

1. a plant extract composition for preparing antihypertensive drug is characterized in that the composition is composed of Qianluo nuclear extract, hawthorn nuclear extract and euphorbia helioscopia extract;
the dosage ratio of the Qianluo extract, the hawthorn seed extract and the euphorbia helioscopia extract is 10:4:3 by weight;
the preparation method of the Qianluo nuclear extract comprises the following steps:
pulverizing semen Qianluo, sieving, placing in round bottom flask, adding 8-10 times of 70% ethanol, heating and reflux extracting in electric heating jacket for 2 times (2 hr and 1 hr for each time), filtering, mixing filtrates, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain semen Qianluo extract;
the preparation method of the hawthorn seed extract comprises the following steps:
taking hawthorn seeds, crushing, sieving, soaking for 24h, filtering, placing in a round-bottom flask, adding 16 times of water, adjusting the pH to 5.0 with hydrochloric acid, adding 0.8% cellulase, placing in a water bath kettle, performing enzymolysis at a constant temperature of 40 ℃ for 30min, transferring the round-bottom flask into an ultrasonic oscillator for extraction for 2 times, each time for 1.5h, wherein the ultrasonic temperature is 80 ℃ and the ultrasonic frequency is 100KHz, filtering, combining filtrates, concentrating, drying under reduced pressure to obtain a dry extract, crushing, and sieving with a 80-mesh sieve to obtain the hawthorn seed extract;
the preparation method of the euphorbia helioscopia extract comprises the following steps:
drying herba Euphorbiae Helioscopiae, pulverizing, sieving, extracting with supercritical CO2 with entrainer ethanol at solid-to-liquid ratio of 1.5ml/mg, extraction pressure of 20MPa, extraction temperature of 45 deg.C, extraction time of 2 hr, filtering the extractive solution, recovering ethanol with rotary evaporator, concentrating, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain herba Euphorbiae Helioscopiae extract;
the Qian Luo nuclear extract, the hawthorn seed extract and the euphorbia helioscopia extract are selected according to the proportion and are evenly mixed to obtain the plant extract composition.
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芊罗（甘枣叶）;个人图书馆;《http://www.360doc.com/content/16/1117/20/21137025_607359858.shtml》;20161117;第2页 *

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