CN101468155A - Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof - Google Patents
Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof Download PDFInfo
- Publication number
- CN101468155A CN101468155A CNA2007103060466A CN200710306046A CN101468155A CN 101468155 A CN101468155 A CN 101468155A CN A2007103060466 A CNA2007103060466 A CN A2007103060466A CN 200710306046 A CN200710306046 A CN 200710306046A CN 101468155 A CN101468155 A CN 101468155A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- preparation
- medicine composition
- crude drug
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a Chinese medicine composition for treating dementia. The Chinese medicine composition is composed of chuangxiong rhizome extract and gastrodia elata extract. The weight proportion of crude drug is 4:1. The preparing method provided by the invention adopts a resin technique in modern technique of Chinese medicine. A large quantity of impurities are removed and the content of active component is higher. The invention also provides a pharmaceutical preparation of the Chinese medicinal composition and the application in preparing medicine for preparing various dementia symptoms.
Description
Technical field
The invention belongs to new Chinese medicine research and development field.Relate to the Chinese medicine composition of the higher treatment dementia of a kind of active constituent content, and its preparation method, preparation and purposes are provided.
Background technology
(Vascular dementia VD) is meant the disordered brain function that various cerebrovascular disease cause and the acquired intelligence infringement syndrome that produces to vascular dementia, is a chronic progressive disease.Be based on memory, cognitive function is damaged, or with the persistence infringement of the acquired intelligence of language, visual space technical ability and emotion or personality disorder.In the dementia that world old people is suffered from, the VD sickness rate accounts for 10%～50%, is only second to Alzheimer's disease (AD).China's VD sickness rate accounts for dull-witted more than 60%, more and more becomes the commonly encountered diseases, the frequently-occurring disease that influence middle-aged and elderly people Health and Living quality.It is reported that about 30% patients with cerebrovascular disease is with the disturbance of intelligence of varying degree, severe patient not only influences patient's life quality, and has increased the financial burden of society and family.Therefore, VD has become global emphasis public health problem, more and more causes people's attention, prevents and treats alzheimer disease, improves the people's life quality, has important practical significance.
Rhizoma Chuanxiong is the dry rhizome of samphire Rhizoma Chuanxiong (Ligusticum shuanxiong Hort.), and acrid in the mouth, warm in nature has the effect of blood-activating and qi-promoting, wind-expelling pain-stopping.Rhizoma Chuanxiong beginning of being used as medicine is stated from Shennong's Herbal, classifies as top gradely, cures mainly apoplexy and goes into brain headache.Put down in writing in the Compendium of Material Medica: Rhizoma Chuanxiong, the gas medicine also in the blood.Blood deficiency, stagnation of QI person should it.Ancient Times in China has been accumulated abundant data to the clinical practice of Rhizoma Chuanxiong single medicinal material and prescription thereof, and successive dynasties book on Chinese herbal medicine and medical book all are documented as " Variorum of Shennong ", " Newly Revised Canon of Materia Medica ", " Zhenglei Bencao ", " Bencao Congxin " etc.
Modern study shows, Rhizoma Chuanxiong all has effect to cardio-cerebrovascular, respiratory system, digestive system, urinary system, bone marrow hematogenesis and aspect such as anticancer.Its effective ingredient ferulic acid has the oxygen-derived free radicals of removing, alleviates Ca
2+Overload, protection vascular endothelial cell, blood vessel dilating, inhibition apoptosis and hypertrophy, antiinflammatory, adjusting immune state, antiplatelet aggregation and thrombosis and influence the effects such as expression of the various kinds of cell factor, thus can play a role at multisystem, many organs, various disease conditions.
Rhizoma Gastrodiae is the dry tuber of orchid Rhizoma Gastrodiae (Gastrodia elata Bl.), sweet in the mouth, suffering, and property is flat, is rare Chinese medicine commonly used.This medicine has the effect of suppressing the hyperactive liver to relieve the wind syndrome, relieving convulsion, be used for the treatment of have a dizzy spell, disease such as infantile convulsion, epilepsy, numb limbs and tense tendons and hemiplegia.Put down in writing in the Compendium of Material Medica: Rhizoma Gastrodiae, be the Liver Channel edema caused by disorder of QI medicine also.Put down in writing in the Jing-Yue Complete Works: control the empty dizzy head of wind and revolve, all rheumatism numbness, bone and muscle strengthening, promoting blood circulation, spasmolytic are probably in a trance.
Modern study shows, Rhizoma Gastrodiae has sedation and analgesia, convulsion, defying age, improves learning and memory, antagonism toxicity of excitatory amino acid, antiinflammatory, immunomodulating, fall blood, decreased heart rate, increase cardiac output, reduce effects such as myocardial oxygen consumption.The main effective ingredient of Rhizoma Gastrodiae is a gastrodine, and content is up to 0.33%～0.67%, and the activity that the pharmacological action of Rhizoma Gastrodiae mostly is gastrodine plays a role.
Modern study thinks that VD has potential control property, is only dementia of preventing and treating in the alzheimer disease, is called as " cataphrenia ".Though modern medicine is familiar with to some extent to its pathogeny, present sure method and the medicine of inefficacy still.The traditional Chinese medical science early has understanding to dementia, and its unique theoretical system and abundant medicine practice are for the treatment of primary disease provides valuable experience.But the Chinese medicine preparation that present inefficacy is still affirmed, can be promoted the use of.The present invention makes its active constituent content higher respectively with the extracts active ingredients and the purification of Rhizoma Chuanxiong and Rhizoma Gastrodiae, and is that 4:1 is combined into Chinese medicine composition with it by proportioning, is used for treatment of vascular dementia and other dementia disease.
Summary of the invention
One of the technical problem to be solved in the present invention is the Chinese medicine composition that development is made up of Rhizoma Chuanxiong extract and Rhizoma Gastrodiae extract.The Chinese medicine composition of the present invention's development, the active constituent content height, impurity is few, good effect, the dose of clinical use significantly reduces, and easy to control the quality.The minimizing of impurity not only can improve the moisture absorption that Chinese patent medicine often has, and makes drug effect remarkable, reduces the side effect that some impurity causes, and increases the compliance that the patient takes medicine.
The technical problem to be solved in the present invention also relates to the preparation technology who develops this Chinese medicine composition, and this method can improve active component content, removes a large amount of impurity, repeated simultaneously, good stability, and the yield height of raw material, and cost is low, is fit to suitability for industrialized production.
The present invention also further provides Chinese medicine preparation and the application in the dull-witted medicine of preparation treatment thereof that contains above-mentioned Chinese medicine composition.
For solving the problems of the technologies described above, the present invention has studied and defined following technical scheme.
The used Rhizoma Chuanxiong of the present invention is the dry rhizome of samphire Rhizoma Chuanxiong (Ligusticum shuanxiong Hort.), and Rhizoma Gastrodiae is the dry tuber of orchid Rhizoma Gastrodiae (Gastrodia elata Bl.).The inventor is through years of researches, with Rhizoma Chuanxiong and Rhizoma Gastrodiae is raw material, pass through solvent extraction, macroporous resin adsorption, solvent elution, eluent concentrate drying respectively, obtain Rhizoma Chuanxiong extract and Rhizoma Gastrodiae extract, and be that 4:1 forms Chinese medicine composition with the crude drug weight proportion.Contain Rhizoma Chuanxiong total phenolic acids in this Chinese medicine composition and Rhizoma Gastrodiae total glycosides weight sum percentage ratio is more than 35%.Mainly contain ferulic acid, chuanxingol, chrysophanol, original pair tea phenol, vanillic acid etc., ligustrazine, gastrodine, salicylic alcohol, hydroxy benzaldehyde, Rhizoma Gastrodiae Fructus Citri Limoniae three fat, succinic acid, daucosterol, citric acid, cupreol etc. in this Chinese medicine composition.
Embodiment of the present invention, Chinese medicine composition prepares by following method:
Get Rhizoma Chuanxiong or Rhizoma Gastrodiae medical material, add and be equivalent to 4～16 times of crude drug weighing body hydrops or 10～90% ethanol extractions, behind the concentrating under reduced pressure, it is 0.1～1g/ml that adding distil water is dissolved to crude drug concentration, last macroporous adsorbent resin static adsorption 2～12 hours, after the washing remove impurity, water or 10～90% ethanol elutions are collected eluent, the concentrating under reduced pressure drying, get Rhizoma Chuanxiong extract or Rhizoma Gastrodiae extract, Rhizoma Chuanxiong extract is mixed promptly getting Chinese medicine composition with Rhizoma Gastrodiae extract.
The preferred embodiment of the invention, Chinese medicine composition prepares by following method:
(1) preparation of Rhizoma Chuanxiong extract: after the pulverizing medicinal materials, add 70～90% ethanol extractions that are equivalent to 10～12 times of crude drug bulking values, behind the concentrating under reduced pressure, it is 0.2～0.3g/ml that adding distil water is dissolved to crude drug concentration, and last macroporous adsorbent resin static adsorption 6～8 hours is after the washing remove impurity, water or 50～90% ethanol elutions, collect eluent, the concentrating under reduced pressure drying promptly gets Rhizoma Chuanxiong extract.
(2) preparation of Rhizoma Gastrodiae extract: after the pulverizing medicinal materials, add 10～30% ethanol extractions that are equivalent to 6～10 times of crude drug bulking values, behind the concentrating under reduced pressure, it is 0.4～0.6g/ml that adding distil water is dissolved to crude drug concentration, and last macroporous adsorbent resin static adsorption 6 hours is after the washing remove impurity, water or 10～50% ethanol elutions, collect eluent, the concentrating under reduced pressure drying promptly gets Rhizoma Gastrodiae extract.
(3) (1) and (2) being pressed crude drug weight 4:1 mixes promptly.
Described extracting method comprises decoction, refluxes, and dipping, percolation etc. are preferably reflux, extract.
Described macroporous resin model can be D101, AB-8, X-5 or NKA-9.The preparation of Rhizoma Chuanxiong extract is preferably D101, and the preparation of Rhizoma Gastrodiae extract is preferably AB-8
Described ethanol elution speed is 2～4BV/h.
The drying means of institute's art is drying under reduced pressure, vacuum drying, lyophilization, spray drying etc., is preferably vacuum drying or lyophilization.
Advantage of the present invention: it is the Chinese medicine composition that raw material is formed that the present invention has developed with Rhizoma Chuanxiong and Rhizoma Gastrodiae two flavor Chinese medicines, adopted the resin technology in the modernization of Chinese medicine technology to be prepared into higher Rhizoma Chuanxiong extract of active constituent content and Rhizoma Gastrodiae extract Rhizoma Chuanxiong and Rhizoma Gastrodiae respectively first, and be that 4:1 has formed Chinese medicine composition by the crude drug proportioning, a large amount of impurity have been removed, the content of active component is higher, and cost is low, and technology is simple, is more suitable for industrialized great production.
On this basis, the present invention has has also researched and proposed the dull-witted Chinese medicine preparation that is used for the treatment of that contains above-mentioned Chinese medicine composition, this Chinese medicine preparation can be that this Chinese medicine composition and pharmaceutically acceptable carrier mix, and pharmaceutically acceptable carrier is the adjuvant of oral formulations adjuvant or parenteral administration.
According to technical scheme of the present invention, this Chinese medicine preparation can be oral formulations or injection preparation, and wherein oral formulations comprises drop pill, capsule, soft capsule, granule, oral liquid, tablet etc.Injection type is injection or lyophilized injectable powder.Used adjuvant comprises: conventional adjuvants such as starch, sucrose, lactose, Icing Sugar, glucose, mannitol, xylitol, Polyethylene Glycol, isopropyl alcohol, soil temperature-80, glycerol, propylene glycol, microcrystalline Cellulose sodium, dextrin, cyclodextrin, sodium chloride, vitamin C, cysteine, citric acid, sodium thiosulfate, sodium sulfite, stearate and gelatin, preparation later stage preparation technology and equipment all belong to the routine techniques of pharmaceutical field, the present invention does not limit this, so will not describe in detail at this.
Observe by research, Chinese medicine composition of the present invention to dementia particularly vascular dementia better therapeutic effect is arranged.For the ease of understanding the therapeutic effect of this extract, the inventor adopts the Chinese medicine composition (Rhizoma Chuanxiong total phenolic acids and Rhizoma Gastrodiae total glycosides content sum are 37.77%) of embodiment 1 method preparation to carry out following pharmacodynamics test:
Test example 1 rat Morris water maze laboratory
100 employing diving towers of extracting male Wistar rat method is carried out learning and memory test, according to the learning and memory achievement rat is divided into 90 of 10 of sham operated rats and model group.Adopt permanent ligation bilateral common carotid arteries (2-VO) legal system to make the VD rat model.6 week of modeling, the back was according to the diving tower achievement, rat is divided into 6 groups, 10 of sham operated rats (normal saline 1ml/100g), (((0.75g crude drug/kg), Dihydroergotoxine (are made into the suspension that concentration is 0.12mg/ml to dosage group in 3.00g crude drug/kg), the Chinese medicine composition for 1.50g crude drug/kg), Chinese medicine composition low dose group for model group (normal saline 1ml/100g), Chinese medicine composition high dose group, 0.6mg/kg) each 10, in the 7th week after the modeling, every day, gastric infusion was 1 time, continuous 4 weeks.Rat Morris water maze laboratory is divided into orientation navigation experiment and space exploration experiment.
(1) orientation navigation experiment
The Morris water maze is a circular rustless steel pond, diameter 120cm, high 50cm.By the orientation branch 4 the different quadrants in all directions are arranged, be equipped with the automatic camera device directly over the pond.During experiment, water temperature is controlled at 25 ± 2 ℃, and laboratory is kept quite, and window hides with light tight curtain, and is indoor with constant fluorescent lamp lighting, the brightness equalization, and peripheral pool object of reference position remains unchanged, and the experimenter wears lab-gown and remains unchanged in quadrant position, north and south.Test and do not establish security platform by the rat free swimming in first day, the upper and lower noon each once, to conform.Beginning in second day is located at Northeast Quadrant center underwater 2cm with the security platform of diameter 11cm, high 29cm, from pool wall 20cm place, rat is put into the pond from western quadrant in the face of pool wall, seeks platform.Training every day is once trained 100s, continuous 5 days for every at every turn.If rat do not find platform as yet in 100s, then rat is put the 10s that stands to the platform, record incubation period is 100s; If rat is found platform in 100s, also be allowed to condition at the 10s that stands on the platform, write down incubation period (rat is found the time of platform), experimental result is as follows:
Table 1 orientation navigation experiment result (x ± s)
Compare with Sham
*P＜0.05,
* *P＜0.001; Compare with Model
#P＜0.05
Experimental result shows: with the increase of training natural law, incubation period is on a declining curve.Trained the 4th day, model group and sham operated rats relatively increase (P＜0.05) incubation period; Administration group and model group relatively, Chinese medicine composition high dose group there was no significant difference incubation period is in the Chinese medicine composition, low dose group shortens (P＜0.05), Dihydroergotoxine group there was no significant difference incubation period incubation period.Trained the 5th day, model group and sham operated rats relatively significantly increase (P＜0.001) incubation period; Administration group and model group relatively, Chinese medicine composition high dose group there was no significant difference incubation period is in the Chinese medicine composition, low dose group shortens (P＜0.05), Dihydroergotoxine group there was no significant difference incubation period incubation period.
(2) space exploration experiment
Training the 6th day, security platform is removed the platform in rat being sought remember under the situation of no platform.Every day training once, every each training 100s, data acquisition of Morris water maze and analysis software write down incubation period the time of platform (for the first time through), cross the platform number of times number of times of platform (in the 100s through) and search strategy automatically.Experimental result is as follows:
Table 2 space exploration experimental result (x ± s)
Compare with Sham
*P＜0.05,
* *P＜0.001; Compare with Model
#P＜0.05; Compare with the HDG group
▲P＜0.05
Experimental result shows: model group and sham operated rats relatively significantly increase (P＜0.001) incubation period, cross the platform number of times and reduce (P＜0.05).Administration group and model group relatively Chinese medicine composition high dose group incubation period, are crossed the platform number of times there are no significant difference; Dosage group incubation period in the Chinese medicine composition, cross the platform number of times there are no significant difference; Chinese medicine composition low dose group shortening incubation period (P＜0.05), mistake platform number of times obviously reduce (P＜0.01); Dihydroergotoxine group incubation period, cross platform number of times there was no significant difference; Dihydroergotoxine group and Chinese medicine composition low dose group compare, and there was no significant difference was crossed the platform number of times and was less than Chinese medicine composition low dosage (P＜0.05) incubation period
The experiment of test example 2 rat shuttle box
Rat shuttle box experimental provision size is 50cm * 16cm * 18cm, the stainless steel bar of case bottom grid for switching on, and central part has the baffle plate of a high 1.2cm at the bottom of the case, and the case bottom is separated into the left and right sides.The buzz controller is arranged at the experimental box top.During training, rat is put into any side of case, begins to be buzz behind the 5s, continue 5s, in the back 10s to electricity irritation (100V, 0.2mA, 50Hz).At first, animal only responds to electric shock, promptly escapes to shock by electricity to avoid to offside.Behind the 5s once more Conditions stimulate and continue impose electricity irritation in animal place side, force animal to escape to opposite side ... so shuttle.When buzz is current, rat escapes to the offside place of safety promptly to think conditioned response (active avoidance response) to have occurred to avoid electric shock immediately.Train every day one time, 20 times every time, trained altogether 7 days.But self-recording unit continuous record rat is subjected to number of shocks, electric shock time and active escape time (time of escaping in the buzzing 5s).Experimental result is as follows:
(1) number of shocks test result
Table 3 rat shuttle box experiment number of shocks test result (x ± s)
Compare with Sham
*P＜0.05,
*P＜0.01,
* *P＜0.001; Compare with Model
#P＜0.05
Experimental result shows: with the increase of training natural law, number of shocks is on a declining curve.Trained preceding 4 days, model group and sham operated rats comparison, administration group and model group relatively, number of shocks there are no significant difference.Trained the 5th day, model group and sham operated rats compare, and number of shocks increases (P＜0.05); Administration group and model group relatively, the high, medium and low dosage group of Chinese medicine composition, Dihydroergotoxine group number of shocks there are no significant difference.Trained the 6th day, model group and sham operated rats compare, and number of shocks obviously increases (P＜0.01); Administration group and model group relatively, the high, medium and low dosage group of Chinese medicine composition number of shocks there are no significant difference, Dihydroergotoxine group number of shocks reduces (P＜0.05).Trained the 7th day, model group and sham operated rats compare, and number of shocks significantly increases (P＜0.001); Administration group and model group relatively, the Chinese medicine composition object height, in, dosage group number of shocks there are no significant difference, Chinese medicine composition low dose group number of shocks reduces (P＜0.05), Dihydroergotoxine group number of shocks reduces (P＜0.05)
(2) electric shock time test result
Table 4 rat shuttle box experiment electric shock time test result (x ± s)
Compare with sham operated rats
*P＜0.01; Compare with model group
#P＜0.05,
##P＜0.01
Experimental result shows: with the increase of training natural law, the electric shock time is shortening trend.Trained preceding 4 days, model group and sham operated rats comparison, administration group and model group relatively, number of shocks there are no significant difference.Trained the 5th day, model group and sham operated rats compare, and the electric shock time obviously increases (P＜0.01); Administration group and model group relatively, the high, medium and low dosage group of Chinese medicine composition, Dihydroergotoxine group number of shocks there are no significant difference.Trained the 6th day, model group and sham operated rats compare, and the electric shock time obviously increases (P＜0.01); Administration group and model group are relatively, Chinese medicine composition high dose group electric shock time there was no significant difference, the dosage group electric shock time shortens (P＜0.05) in the Chinese medicine composition, and the Chinese medicine composition low dose group electric shock time shortens (P＜0.05), and the Dihydroergotoxine group electric shock time shortens (P＜0.05).Trained the 7th day, model group and sham operated rats compare, and the electric shock time obviously increases (P＜0.01); Administration group and model group are relatively, Chinese medicine composition high dose group electric shock time there was no significant difference, the dosage group electric shock time shortens (P＜0.05) in the Chinese medicine composition, and the Chinese medicine composition low dose group electric shock time obviously shortens (P＜0.01), and the Dihydroergotoxine group electric shock time shortens (P＜0.05).
(3) initiatively escape the time test result
Time test result (x ± s) is initiatively escaped in table 5 rat shuttle box experiment
Compare with sham operated rats
*P＜0.01; Compare with model group
#P＜0.05
Experimental result shows: with the increase of training natural law, initiatively the escape time is increase trend.Trained preceding 5 days, model group and sham operated rats comparison, administration group and model group relatively, number of shocks there are no significant difference.Trained the 6th day, model group and sham operated rats compare, and initiatively the escape time shortens (P＜0.05); Administration group and model group compare, Chinese medicine composition object height, middle dosage group electric shock time there was no significant difference, and the Chinese medicine composition low dose group is escape time increase (P＜0.05) initiatively, and initiatively the escape time increases (P＜0.05) to the Dihydroergotoxine group.Trained the 7th day, model group and sham operated rats compare, and initiatively the escape time shortens (P＜0.05); Administration group and model group compare, Chinese medicine composition object height, middle dosage group electric shock time there was no significant difference, and the Chinese medicine composition low dose group is escape time increase (P＜0.05) initiatively, and the Dihydroergotoxine group is initiatively escaped the time there was no significant difference.
3 one kinds of influences for the treatment of dull-witted Chinese medicine composition of test example to VD rat cholinergic nerve system
Rat is finished the back sacrificed by decapitation in behavioristics's experiment; separate the rat skull; on ice pan, take out brain rapidly; the center symmetry is cut; get the right side cerebral tissue, accurately claim after the weight in wet base cerebral tissue that takes out to be put into the glass homogenate pipe of pre-cooling, the normal saline that adds 9 times of amounts is made 10% brain tissue homogenate; measure (Ach) content of acetylcholine in the rat cerebral tissue and choline acetylase (ChAT) activity respectively, the result is as follows:
(1) Ach content in the rat cerebral tissue
Ach content in table 6 rat cerebral tissue (x ± s)
Compare with Sham
* *P＜0.001; Compare with Model
#P＜0.05
Experimental result shows: model group and sham operated rats compare, and Ach content significantly reduces (P＜0.001); Administration group and model group relatively, Chinese medicine composition high dose group Ach content there was no significant difference is in the Chinese medicine composition, low dose group and Dihydroergotoxine group Ach content raise (P＜0.05); With the Dihydroergotoxine group relatively, in the Chinese medicine composition, low dose group Ach content there was no significant difference
(2) ChAT activity in the rat cerebral tissue
ChAT activity in table 7 rat cerebral tissue (x ± s)
Compare with Sham
*P＜0.05; Compare with Model
#P＜0.05
Experimental result shows: model group and sham operated rats compare, active reduce (P＜0.05) of ChAT; Administration group and model group are relatively, Chinese medicine composition high dose group ChAT is active to raise, but statistical result there was no significant difference, active raise (P＜0.05) of dosage group ChAT in the Chinese medicine composition, Chinese medicine composition low dose group ChAT is active to raise (P＜0.05), active raise (P＜0.05) of Dihydroergotoxine group ChAT.With the Dihydroergotoxine group relatively, in the Chinese medicine composition, the active there was no significant difference of low dose group ChAT.
The specific embodiment
Describe the enforcement of technical solution of the present invention by the following examples in detail, but should not limit practical range of the present invention with this.
Embodiment 1: a kind of preferred for preparation method for the treatment of dull-witted Chinese medicine composition
(1) preparation of Rhizoma Chuanxiong extract: get 800g Rhizoma Chuanxiong medical material, add 90% alcohol reflux 2 times that is equivalent to 12 times of crude drug bulking values after the pulverizing, each 3 hours, behind the concentrating under reduced pressure, it was 0.25g/ml that adding distil water is dissolved to crude drug concentration, last D101 macroporous adsorbent resin static adsorption 6 hours, after the washing remove impurity, water or 50% ethanol elution are collected eluent, the concentrating under reduced pressure vacuum drying promptly gets Rhizoma Chuanxiong extract.
(2) preparation of Rhizoma Gastrodiae extract: get 200g Rhizoma Gastrodiae medical material, add 10% alcohol reflux 3 times that is equivalent to 8 times of crude drug bulking values after the pulverizing, behind the each 3 hours concentrating under reduced pressure, it is 0.5g/ml that adding distil water is dissolved to crude drug concentration, and last AB-8 macroporous adsorbent resin static adsorption 6 hours is after the washing remove impurity, water or 10% ethanol elution, collect eluent, the concentrating under reduced pressure vacuum drying promptly gets Rhizoma Gastrodiae extract.
(3) (1) and (2) mixing is promptly got Chinese medicine composition 38.75g, Rhizoma Chuanxiong total phenolic acids and Rhizoma Gastrodiae total glycosides content reach 37.77%.
Embodiment 2: a kind of preferred for preparation method for the treatment of dull-witted Chinese medicine composition
(1) preparation of Rhizoma Chuanxiong extract: get 800g Rhizoma Chuanxiong medical material, add 80% alcohol reflux 2 times that is equivalent to 10 times of crude drug bulking values after the pulverizing, each 3 hours, behind the concentrating under reduced pressure, it was 0.2g/ml that adding distil water is dissolved to crude drug concentration, last D101 macroporous adsorbent resin static adsorption 6 hours, after the washing remove impurity, water or 60% ethanol elution are collected eluent, the concentrating under reduced pressure vacuum drying promptly gets Rhizoma Chuanxiong extract.
(2) preparation of Rhizoma Gastrodiae extract: get 200g Rhizoma Gastrodiae medical material, add 20% alcohol reflux 3 times that is equivalent to 10 times of crude drug bulking values after the pulverizing, behind the each 3 hours concentrating under reduced pressure, it is 0.4g/ml that adding distil water is dissolved to crude drug concentration, and last AB-8 macroporous adsorbent resin static adsorption 6 hours is after the washing remove impurity, water or 50% ethanol elution, collect eluent, the concentrating under reduced pressure vacuum drying promptly gets Rhizoma Gastrodiae extract.
(3) (1) and (2) mixing is promptly got Chinese medicine composition 37.54g, Rhizoma Chuanxiong total phenolic acids and Rhizoma Gastrodiae total glycosides content reach 36.06%.
Embodiment 3: a kind of Chinese medicine composition preferred dosage form drop pill for the treatment of dementia
Get the thick paste (relative density is 1.30～1.32) of the Chinese medicine composition mixing of embodiment 1 method preparation, join (ratio of PEG4000 and PEG6000 is 1:5) mix homogeneously in the molten matrix in the 7:1 ratio, pour in the drop pill machine under 96 ℃ of insulations, the speed of dripping with 60dmin-1 splashes in the liquid paraffin condensing agent and (drips apart from 5cm), cooling is solidified into ball, takes out, dry, promptly.
Embodiment 4: a kind of Chinese medicine composition preferred dosage form capsule for the treatment of dementia
Get the Chinese medicine composition 100g of embodiment 1 method preparation, 60 ℃ of dryings grind, and cross 80 mesh sieves, the medical starch 98g and the magnesium stearate 2g that added 80 mesh sieves, mix homogeneously is made soft material in right amount with 85% ethanol, crosses 30 mesh sieve system granules, oven dry, make moisture less than 5%, cross 40 orders and shine granulate, be sub-packed in No. 3 capsules.Use aluminium plastic composite packaging, promptly.
Embodiment 5: a kind of Chinese medicine composition preferred dosage form tablet for the treatment of dementia
Get the Chinese medicine composition 30g of embodiment 1 method preparation, add starch 30g, microcrystalline Cellulose 10g granulates with 12 mesh sieves in right amount with 10% starch slurry, and dry below 55 ℃, dry granular adds magnesium stearate, granulate, and mixing, tabletting, promptly.
Embodiment 6: a kind of Chinese medicine composition preferred dosage form lyophilized injectable powder for the treatment of dementia
Get the Chinese medicine composition 100g of embodiment 4 methods preparation, add an amount of water for injection, after the stirring and dissolving, ultrafiltration obtains apyrogenic clear liquor, adds the injection water to 1000ml, is packed as 1000, presses freeze-dried powder preparation technology lyophilizing, makes lyophilized injectable powder.
Claims (10)
1. treat dull-witted Chinese medicine composition for one kind, it is characterized in that: this Chinese medicine composition is that 4:1 forms by Rhizoma Chuanxiong extract and Rhizoma Gastrodiae extract by the crude drug weight ratio.
2. Chinese medicine composition according to claim 1 is characterized in that: Rhizoma Chuanxiong total phenolic acids and Rhizoma Gastrodiae total glycosides content sum are greater than 35% in this Chinese medicine composition.
3. the preparation method of Chinese medicine composition according to claim 1 and 2, it is characterized in that: get Rhizoma Chuanxiong or Rhizoma Gastrodiae medical material, add and be equivalent to 4～16 times of crude drug weighing body hydrops or 10～95% ethanol extractions, behind the concentrating under reduced pressure, it is 0.1～1g/ml that adding distil water is dissolved to crude drug concentration, last macroporous adsorbent resin static adsorption 2～12 hours, after the washing remove impurity, water or 10～90% ethanol elutions, collect eluent, the concentrating under reduced pressure drying gets Rhizoma Chuanxiong extract or Rhizoma Gastrodiae extract, Rhizoma Chuanxiong extract is mixed promptly getting Chinese medicine composition with Rhizoma Gastrodiae extract.
4. the preparation method of Chinese medicine composition according to claim 3 is characterized in that:
(1) preparation of Rhizoma Chuanxiong extract: after the pulverizing medicinal materials, add 70～90% ethanol extractions that are equivalent to 10～12 times of crude drug bulking values, behind the concentrating under reduced pressure, it is 0.2～0.3g/ml that adding distil water is dissolved to crude drug concentration, and last macroporous adsorbent resin static adsorption 6～8 hours is after the washing remove impurity, water or 50～90% ethanol elutions, collect eluent, the concentrating under reduced pressure drying promptly gets Rhizoma Chuanxiong extract;
(2) preparation of Rhizoma Gastrodiae extract: after the pulverizing medicinal materials, add 10～30% ethanol extractions that are equivalent to 6～10 times of crude drug bulking values, behind the concentrating under reduced pressure, it is 0.4～0.6g/ml that adding distil water is dissolved to crude drug concentration, and last macroporous adsorbent resin static adsorption 6 hours is after the washing remove impurity, water or 10～50% ethanol elutions, collect eluent, the concentrating under reduced pressure drying promptly gets Rhizoma Gastrodiae extract;
(3) (1) and (2) being pressed crude drug weight 4:1 mixes promptly.
5. according to the preparation method of claim 3 or 4 described Chinese medicine compositions, it is characterized in that extracting method is backflow, decoction, dipping or percolation.
6. according to the preparation method of claim 3 or 4 described Chinese medicine compositions, it is characterized in that described macroporous resin model is D101, AB-8, X-5 or NKA-9.
7. treat dull-witted Chinese medicine preparation for one kind, it is characterized in that containing described Chinese medicine composition of arbitrary claim and acceptable accessories composition in the claim 1～6.
8. according to claim 7 described a kind of dull-witted Chinese medicine preparation for the treatment of, it is characterized in that this Chinese medicine preparation can be drop pill, capsule, soft capsule, granule, oral liquid, tablet, injection or injectable powder.
9. described Chinese medicine composition of claim 1～8 or the Chinese medicine preparation application in the dull-witted medicine of preparation treatment.
10. described Chinese medicine composition of claim 9 or the Chinese medicine preparation application in the dull-witted medicine of preparation treatment is characterized in that the application in preparation treatment vascular dementia medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007103060466A CN101468155A (en) | 2007-12-29 | 2007-12-29 | Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007103060466A CN101468155A (en) | 2007-12-29 | 2007-12-29 | Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101468155A true CN101468155A (en) | 2009-07-01 |
Family
ID=40826118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007103060466A Pending CN101468155A (en) | 2007-12-29 | 2007-12-29 | Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101468155A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793708A (en) * | 2011-05-27 | 2012-11-28 | 江西中医学院 | Application of composition of gastrodin and ferulic acid in preparation of medicament for preventing and treating senile dementia |
| CN102885985A (en) * | 2012-11-02 | 2013-01-23 | 北京联合大学生物化学工程学院 | Preparation method and application of rhizoma gastrodiae and rhizoma chuanxiong compound extract |
| CN104000840A (en) * | 2014-06-16 | 2014-08-27 | 四川中医药高等专科学校 | Preparation method of extractive containing gastrodin and gastrodia elata polysaccharide |
| CN105748951A (en) * | 2014-12-16 | 2016-07-13 | 中国科学院大连化学物理研究所 | Rhizoma gastrodiae extract for improving sleep and application thereof |
| CN113332386A (en) * | 2021-06-01 | 2021-09-03 | 深圳利孚生物科技有限公司 | Composition for improving memory and preparation method thereof |
-
2007
- 2007-12-29 CN CNA2007103060466A patent/CN101468155A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793708A (en) * | 2011-05-27 | 2012-11-28 | 江西中医学院 | Application of composition of gastrodin and ferulic acid in preparation of medicament for preventing and treating senile dementia |
| CN102885985A (en) * | 2012-11-02 | 2013-01-23 | 北京联合大学生物化学工程学院 | Preparation method and application of rhizoma gastrodiae and rhizoma chuanxiong compound extract |
| CN104000840A (en) * | 2014-06-16 | 2014-08-27 | 四川中医药高等专科学校 | Preparation method of extractive containing gastrodin and gastrodia elata polysaccharide |
| CN105748951A (en) * | 2014-12-16 | 2016-07-13 | 中国科学院大连化学物理研究所 | Rhizoma gastrodiae extract for improving sleep and application thereof |
| CN113332386A (en) * | 2021-06-01 | 2021-09-03 | 深圳利孚生物科技有限公司 | Composition for improving memory and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101229279A (en) | Collateral-dredging antihyperlipidemic product containing dark tea active substance and preparing method thereof | |
| CN101249112A (en) | Fat-reducing products containing black tea Eurotium Cristatum and preparation thereof | |
| CN104706855A (en) | Preparation methods and application of Jichuan decoction traditional Chinese medicine extract | |
| US20220226413A1 (en) | Traditional chinese medicine composition with improving cognition effect, preparation method thereof and traditional chinese medicine preparation | |
| CN111743934A (en) | Traditional Chinese medicine composition for regulating intestinal flora and application thereof | |
| CN101468155A (en) | Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof | |
| CN104663975A (en) | Acanthopanax health tea for decreasing sugar and lipid and preparation method for acanthopanax health tea | |
| CN104758806A (en) | Decocted extract for treating coronary heart disease and preparation method thereof | |
| CN104223297B (en) | A kind of schisandra chinensis health-care oral liquid of slow down aging and preparation method thereof | |
| CN103142916B (en) | Medicament for preventing and treating senile dementia and preparation method thereof | |
| CN102847061B (en) | Traditional Chinese medicine preparation for treating pediatric viral myocarditis and preparation method thereof | |
| CN104206595A (en) | Blood lipid and blood glucose reducing Chinese wolfberry root-bark health care tea and preparation method thereof | |
| CN103735973A (en) | Traditional Chinese medicine composition for improving memory and preparation method thereof | |
| CN103735621B (en) | A kind of Chinese medicine composition with blood fat reducing and enhancing immunity effect | |
| CN110772564A (en) | Traditional Chinese medicine extract composition with depression mood regulating effect, preparation method thereof and traditional Chinese medicine preparation | |
| CN101274013B (en) | Prescription medicine for curing senile dementia and preparation | |
| CN1679698A (en) | Medicinal preparation containing notoginseng and lovge rhizome for treating cardio-cerebral blood vessel diseases and its preparing method | |
| CN101919924B (en) | Pharmaceutical composition for curing tristimania and preparation method and application thereof | |
| CN104491075A (en) | Refined part of total gardenia crocin with anti-depression effect as well as preparation method and application of refined part | |
| CN114098085A (en) | Functional food composition with auxiliary memory improving function and application | |
| CN108295131B (en) | Plant extract composition with blood pressure reducing effect, preparation method and application thereof | |
| CN103623109B (en) | Traditional Chinese medicine composition for treating ischemic cerebrovascular disease and preparation method thereof | |
| CN1182864C (en) | Medicine for vascular denmentia disease and preparation method | |
| CN104491242A (en) | Medicament composition for treating cognitive dysfunction as well as preparation method and application thereof | |
| CN109123655A (en) | A kind of special doctor's food and preparation method with three high crowd's generation meal regulatory functions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090701 |