CN103735973A - Traditional Chinese medicine composition for improving memory and preparation method thereof - Google Patents
Traditional Chinese medicine composition for improving memory and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for improving memory and a preparation method thereof. The composition is prepared from such medicinal raw materials as panaxadiol glucoside, ophiopogon root, asparagus, spina date seed, platycladi seed, dangshen, radix scrophulariae, dogwood, fructus alpiniae oxyphyllae, semen cuscutae and the like. The invention also provides a preparation method of the traditional Chinese medicine composition for improving memory. For a mouse dosed with the traditional Chinese medicine composition disclosed by the invention, in a step-down test and an Morris water maze test, the number of errors is decreased significantly (P is smaller than 0.05, P is smaller than 0.01), which is obviously superior to that of a control group and has an obvious difference with the control group (P is smaller than 0.05, P is smaller than 0.01), so that the traditional Chinese medicine composition disclosed by the invention can be considered to play a memory perfecting and consolidating effect on the mouse.
Description
Technical field
The present invention relates to pharmaceutical sanitary field, be specifically related to a kind of Chinese medicine composition that improves memory and preparation method thereof.
Background technology
In man memory process, be exactly that Cerebral Cortex Neuronal Cells is actively movable, the process that records and preserve, memory is relevant with the activity of brain cell on cerebral cortex.When carrying out mental activity, brain cell needs a large amount of oxygen.Although only 1.4 kilograms of people's brain weights, its oxygen consumption accounts for 1/5～1/4 of whole body oxygen consumption, is whole body oxygen demand maximum " rich anies influential family ", and institute's energy requirement all will be supplied with by cell.Power work is heavy or when nervous, the oxygen that it is required and nutrient all can increase exponentially, and because brain itself can not be laid in more energy, it needs other histiocytes in body that energy is supplied with it continuously, if supplement and can form brain fag not in time, brain asthenia.
If brain is in asthenia and sub-health state, its memory certainly will be had a greatly reduced quality.Hypomnesis is the inevitable performance brain form of brain fag [8].Therefore, keep the memory function that brain is good, must guarantee that cerebral cortex is pure and fresh active, oxygen supply is abundant.
Obviously, strengthen providing brain with oxygen, relieving brain fatigue is the inevitable choice that improves memory.
At present, the health product that improve memory on market are mainly to take in various nutrient substance, and trace element, as DHA, zinc, ferrum, magnesium etc.Yet, bad if the absorption of trace element is controlled, can affect the absorption of other nutrient substance, for example, take in too much zinc, can affect the absorption of calcium, and affect the formation of skeleton.Studies have shown that, memory and attention have very large associated, and it is with single-hearted devotion to only have, and with breathless interest, information and object just can iron deep trace in cerebral cortex; Otherwise, absent minded, be not intended to note too much, can make people's hypomnesis.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine composition that improves memory;
Another object of the present invention is to provide a kind of Chinese medicine composition preparation method of improving memory.
The object of the invention is to realize by following technical scheme:
The Chinese medicine composition that improves memory of the present invention is to be prepared from by the raw material containing following weight portion:
Panoxadiol's glycosides 5-15 part, Radix Ophiopogonis 20-40 part, Radix Asparagi 20-40 part, Semen Ziziphi Spinosae 15-35 part, Semen Platycladi 15-35 part, Radix Codonopsis 15-35 part, Radix Scrophulariae 15-35 part, Fructus Corni 15-35 part, Fructus Alpiniae Oxyphyllae 15-35 part, Semen Cuscutae 15-35 part.
Preferably, be to be prepared from by the raw material containing following weight portion:
Panoxadiol's glycosides 8-12 part, Radix Ophiopogonis 25-35 part, Radix Asparagi 25-35 part, Semen Ziziphi Spinosae 20-30 part, Semen Platycladi 20-30 part, Radix Codonopsis 20-30 part, Radix Scrophulariae 20-30 part, Fructus Corni 20-30 part, Fructus Alpiniae Oxyphyllae 20-30 part, Semen Cuscutae 20-30 part.
More preferably, be to be prepared from by the raw material containing following weight portion:
10 parts of panoxadiol's glycosides, 30 parts of Radix Ophiopogonis, 30 parts of Radix Asparagis, 25 parts of Semen Ziziphi Spinosaes, 25 parts of Semen Platycladi, 25 parts of Radix Codonopsis, 25 parts of Radix Scrophulariaes, 25 parts of Fructus Corni, 25 parts of Fructus Alpiniae Oxyphyllae, 25 parts of Semen Cuscutae.
Below crude drug source of the present invention:
Panaxadiol saponin: be a kind of of ginsenoside.Ginsenoside (Ginsenoside) is a kind of steroid compound, triterpene saponin.Mainly be present in Araliaceae Panax medical material, Panax's majority is some rare Chinese medicines, as Radix Notoginseng, Radix Ginseng, Radix Panacis Quinquefolii etc.Ginsenoside can be divided into three groups of two classes according to the difference of sapogenin, and a class is the Dammar methane series saponin of tetracyclic triterpene, and wherein one group of acid hydrolysis end product is panoxadiol, as ginsenoside Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rh2 etc.
Radix Ophiopogonis: be liliaceous plant Radix Ophiopogonis (dwarf lilyturf) Ophiopogon japonicus(T.f) dried root of Ker-Gawl..Excavate summer, cleans, and is repeatedly exposed to the sun, banks up, and to dry very likely, removes
fibrous root, dry.[nature and flavor] sweet micro-hardship, cold.[return through] enters lung, stomach, heart channel.[function cures mainly] nourishing YIN and moistening the lung, the relieving restlessness that clears away heart-fire, reinforcing stomach reg fluid.Control dryness of the lung dry cough, spit blood, spitting of blood, consumptive lung disease, lung abscess, asthenia dysphoria with smothery sensation, quenches one's thirst, calentura Tianjin wound, dry mouth and throat, constipation.
Radix Asparagi: this product is liliaceous plant Radix Asparagi Asparagus cochinchinensis(Lour.) dried root of Merr..Autumn, two seasons of winter excavate, and clean, and remove stem foot and fibrous root, put and in boiling water, boil or steam to the saturating heart, remove while hot crust, cleaning, drying.[nature and flavor] are sweet, bitter, cold.[return through] returns lung, kidney channel.[function cures mainly] nourishing YIN to relieve dryness, lung heat clearing is promoted the production of body fluid.For dryness of the lung dry cough, pertussis expectorant is glutinous, dry throat and mouth, dryness of the intestine constipation.
Semen Ziziphi Spinosae: this product is Rhamnaceae plant Ziziphi Spinosae Ziziphus jujuba Mill.var.spinosa(Bunge) dry mature seed of Hu ex H.F.Chou.The time of the year when autumn changes into winter mature fruit of gathering, removes sarcocarp and nucleocapsid, collects seed, dries.[nature and flavor] are sweet, sour, flat.[return through] returns liver, gallbladder, heart channel.[function cures mainly] tonifying liver, mind calming, arresting sweating, promotes the production of body fluid.For restlessness of asrhenia type and insomnia, palpitation with fear dreaminess, the empty hyperhidrosis of body, Tianjin wound is thirsty.
Semen Platycladi: this product is Cupressaceae plant Cacumen Platycladi Platycladus orientalis(L.) dry mature kernal of Franco.The mature seed of gathering in autumn, two seasons of winter, dries, and removes kind of a skin, collects kernel.[nature and flavor] are sweet, flat.[return through] GUIXIN, kidney, large intestine channel.[function cures mainly] tranquilizing by nourishing the heart, hidroschesis, intestine moistening.For fidgets due to deficiency insomnia, severe palpitation, night sweat due to yin deficiency, dryness of the intestine constipation.
Radix Codonopsis: this product is Campanulaceae Radix Codonopsis Codonopsis pilosula(Franch.) Nannf., element flower Radix Codonopsis (RADIX CODONOPSIS PILOSULAE from Northwest of China) Codonopsis pilosula Nannf.var.modesta(Nannf.) dry root of L.T.Shen or radix codonpsis tangshen Codonopsis tangshen Oliv..Excavate autumn, cleans, and dries.[nature and flavor] are sweet, flat.[return through] returns spleen, lung meridian.[function cures mainly] invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.For deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency cough, interior-heat is quenched one's thirst.
Radix Scrophulariae: this product is the dry root of goatweed Radix Scrophulariae Scrophularia ningpoensis Hemsl..Excavate when stem and leaf is withered winter.Remove rhizome, plumelet, fibrous root and silt, shine or be dried to half-driedly, stack 3～6 days, repeatedly for several times to dry.[nature and flavor] are sweet, bitter, salty, are slightly cold.[return through] returns lung, stomach, kidney channel.[function cures mainly] removing heat from blood YIN nourishing, eliminating fire and detoxication.For consumption of YIN caused by febrile disease, crimson tongue excessive thirst, maculae caused by violent heat pathogen, constipation is hindered in Tianjin, hectic fever due to YIN-deficiency chronic cough, conjunctival congestion, pharyngalgia, scrofula, diphtheria, carbuncle sore tumefacting virus.
Fructus Corni: this product is the drying and ripening sarcocarp of Cornaceae plant Fructus Corni Cornus officinalis Sieb.et Zucc..The picking fruit when time of the year when autumn changes into winter, peel reddened, after drying or put and slightly scald in boiling water with slow fire, in time except removal core, dry.[nature and flavor] acid, puckery, tepor.[return through] returns liver, kidney channel.[function cures mainly] liver and kidney tonifying, essence astringing and desertion stemming.For vertigo and tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, bleeding not during menses, profuse sweating collapse.Interior-heat is quenched one's thirst.
Fructus Alpiniae Oxyphyllae: this product is the dry mature fruit of zingiberaceous plant Fructus Alpiniae Oxyphyllae Alpinia oxyphylla Miq..When fruit is by green reddening between summer, autumn, gather, dry or cold drying.[nature and flavor] are pungent, temperature.[return through] returns spleen, kidney channel.[function cures mainly] is warming spleen and stopping diarrha, takes the photograph saliva, warming the kidney, controlling nocturnal emission with astringent drugs reducing urination.For spleen-cold syndrome, have loose bowels, cold type of pain in abdomen, mouthful many salivas, enuresis due to deficiency of the kidney, frequent micturition, seminal emission nebulousurine.
Semen Cuscutae: this product is the dry mature seed of convolvulus cuscuta plant Cuscuta chinensis Lam..The plant of gathering during fruit maturation autumn, dries, and lays seed, removes impurity.[nature and flavor] are sweet, temperature.[return through] returns liver,kidney,spleen warp.[function cures mainly] nourishing the liver and kidney, controlling nocturnal emission with astringent drugs reducing urination, antiabortive, improving eyesight, antidiarrheal.For impotence and seminal emission, dripping urination, enuresis frequent micturition, soreness of the waist and knees, blurred vision tinnitus, vaginal bleeding due to deficiency of the kidney during pregnancy, frequent fetal movement, spleen is suffered from a deficiency of the kidney and is rushed down; Externally treating leucoderma.
Another aspect of the present invention has been to provide the preparation method of the active component of Chinese medicine composition of the present invention, and the method is the ethanol extraction that adopts water extraction or 40-80% concentration expressed in percentage by volume.Concrete preparation method is as follows:
Scheme one: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, and each extraction time is 1-3 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract, obtains described active component.
Scheme two: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, merge extractive liquid,, filters the clear paste that when filtrate is concentrated into 70-80 ℃, relative density is 1.05-1.20, add ethanol, making containing alcohol amount is 40-70%, and standing 12-24 hour filters, filtrate is condensed into fluid extract, obtains described active component.
Scheme three: get whole medical materials, use 40-80% alcohol reflux 2-3 time, doubly, extraction time is 1-3 hour to the 4-10 that each ethanol consumption is medical material total amount, and merge extractive liquid,, filters, and filtrate is condensed into fluid extract, obtains described active component.
The Chinese medicine composition that improves memory of the present invention, can coordinate with pharmaceutically acceptable carrier, makes various common dosage forms, as tablet, granule, capsule, syrup, mixture etc.
Pharmaceutically acceptable carrier of the present invention includes but not limited to following:
Diluent: starch, Icing Sugar, lactose, dextrin, microcrystalline Cellulose, inorganic salt, sugar alcohols etc.
Wetting agent and binding agent: purified water, ethanol, gelatin, Polyethylene Glycol, cellulose derivative etc.
Disintegrating agent: starch, carboxymethyl starch sodium, cellulose derivative, polyvinylpolypyrrolidone etc.
Lubricant: magnesium stearate, micropowder silica gel, Pulvis Talci, Polyethylene Glycol etc.
Cosolvent: water, ethanol, glycerol, propylene glycol, liquid Paraffin, plant wet goods.
Correctives: sucrose, monosaccharide, aromatic etc.
Antiseptic: benzoic acid, sorbic acid, methyl ester, ethyl ester, propyl ester etc.
Method 1: tablet
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add tablet to commonly use adjuvant, production method is prepared into tablet of the present invention routinely.
The conventional adjuvant of above-mentioned tablet comprises one of diluent, wetting agent, binding agent, disintegrating agent, lubricant or whole.
Method 2: granule
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add granule to commonly use adjuvant, production method is prepared into granule of the present invention routinely.
The conventional adjuvant of above-mentioned granule comprises one of diluent, wetting agent, binding agent, disintegrating agent or whole.
Method 3: capsule
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add capsule to commonly use adjuvant, production method is prepared into capsule of the present invention routinely.
The conventional adjuvant of above-mentioned capsule comprises one of diluent, wetting agent, binding agent, disintegrating agent, lubricant or whole.
Method 4: syrup
Get scheme one to one of scheme three gained active component, add syrup to commonly use adjuvant, production method is prepared into syrup of the present invention routinely.
The conventional adjuvant of above-mentioned syrup comprises one of correctives, antiseptic, cosolvent or whole.
Method 5: mixture
Get scheme one to one of scheme three gained active component, add mixture to commonly use adjuvant, production method is prepared into mixture of the present invention routinely.
The conventional adjuvant of above-mentioned mixture comprises one of correctives, antiseptic or whole.
Side separates: we's card is the card of disarmony between the heart and kidney, the deficiency of YIN-blood of the heart, lack of preservation of spirit, holds concurrently and deficiency of kidney yin, brain is hollow, so kidney water can not on help in the heart, heart-fire can not meet at down kidney.Card is by pondering over too, strain heart kidney, and cloudy blood secretly consumes, and due to extreme misery can not help mutually, clinical condition is shown in vexed insomnia, forgetful nocturnal emission.Control suitable YIN nourishing liquid, clearind deficient heat, nourishing the heart to keep a sound mind.We monarch is with Radix Asparagi and Radix Ophiopogonis, and Radix Ophiopogonis can GUIXIN warp, nourishes heart the moon and the heat that clears away heart-fire; Radix Asparagi can be returned kidney channel, nourishing kidney-yin and fall asthenic fire, the two medicines nutrient solution of promoting the production of body fluid again, strong water system fire.The product of Semen Ziziphi Spinosae, all tranquilizing by nourishing the hearts of Semen Platycladi, Semen Ziziphi Spinosae nourishes heart the moon, the liver benefiting blood and calms the nerves, and Semen Platycladi sweet in the mouth matter profit, can nourish again cloudy liquid, the strong water system fire of principal drug assistance; Radix Codonopsis is enriched blood and is promoted the production of body fluid, and in the cloudy blood of the heart, has tonification; Radix Scrophulariae nourishing YIN and clearing away heat, can help again Radix Asparagi to fall fire mutually, is all ministerial drug.Fructus Corni, Fructus Alpiniae Oxyphyllae, Semen Cuscutae all can kidney invigorating and YANG supporting, and Fructus Corni is held concurrently can benefit essence, and it is intended to " treating YIN within YANG, cloudy positive liter, and source is without cease ".All medicines share, and play altogether nourishing YIN and clearing away heat, the merit of tranquilizing by nourishing the heart.
The present invention adds panoxadiol's glycosides in above-mentioned side.Panoxadiol's glycosides has the effect that improves ability of learning and memory, and this main and panoxadiol's glycosides improve brain blood and supply relevantly, and it is also one of its mechanism of improving brain learning memory ability to the removing of oxygen-derived free radicals.Above-mentioned side assists with panoxadiol's glycosides, stronger to the improvement effect of brain memory ability.
A kind of Chinese medicine composition that improves memory provided by the invention has the following advantages:
1, the present invention has nourishing YIN and clearing away heat, and the effect of nourishing YIN and supplementing blood, tranquilizing by nourishing the heart can improve people's attention and memory.Give mice step down test errors number of the present invention and obviously reduce (P<0.01), and have significant difference (P<0.05, P<0.01) between the present invention and comparative example group.The present invention reduces for model group prolongation of latency and errors number, can think test sample to mice be improved, the effect of consolidating memory.
2, in Morris water maze test, compare with comparative example group, give mice of the present invention and obviously shorten (P<0.05, P<0.01) incubation period, errors number obviously reduces (P<0.05, P<0.01).Result shows, the cognitive disorder that the present invention causes mice to scopolamine is improved effect.
3, Chinese medicine composition composition formula of the present invention is rationally innovated, safe and effective, and production cost is low, technique is simple.
The specific embodiment
Below by embodiment, further illustrate the present invention.It should be understood that embodiments of the invention are for the present invention rather than limitation of the present invention are described.The simple modifications that essence according to the present invention is carried out the present invention all belongs to the scope of protection of present invention.
Embodiment 1 syrup
Get panoxadiol's glycosides 10g, Radix Ophiopogonis 30g, Radix Asparagi 30g, Semen Ziziphi Spinosae 25g, Semen Platycladi 25g, Radix Codonopsis 25g, Radix Scrophulariae 25g, Fructus Corni 25g, Fructus Alpiniae Oxyphyllae 25g, Semen Cuscutae 25g.By above-mentioned whole medical materials, extracting in water 2 times, twice amount of water is respectively 10 times, 8 times of medical material gross weight, and the extracted twice time is respectively 2 hours, 1 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.In extractum, add syrup adjuvant sucrose, Mel, potassium sorbate, water to stir, make syrup.
Embodiment 2 tablets
Get panoxadiol's glycosides 8g, Radix Ophiopogonis 25g, Radix Asparagi 25g, Semen Ziziphi Spinosae 20g, Semen Platycladi 20g, Radix Codonopsis 20g, Radix Scrophulariae 20g, Fructus Corni 20g, Fructus Alpiniae Oxyphyllae 20g, Semen Cuscutae 20g.By above-mentioned whole medical materials, with 40% alcohol reflux 3 times, each ethanol consumption is respectively 10 times, 8 times, 4 times of medical material total amount, 3 hours, 2 hours, 1 hour respectively extraction time, merge extractive liquid,, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add supplementary product starch, ethanol, Pulvis Talci, make tablet.
Embodiment 3 mixture
Get panoxadiol's glycosides 12g, Radix Ophiopogonis 35g, Radix Asparagi 35g, Semen Ziziphi Spinosae 30g, Semen Platycladi 30g, Radix Codonopsis 30g, Radix Scrophulariae 30g, Fructus Corni 30g, Fructus Alpiniae Oxyphyllae 30g, Semen Cuscutae 30g.By above-mentioned whole medical materials, extracting in water 3 times, three amount of water are equivalent to respectively 12 times, 8 times, 6 times of medical material gross weight, 3 hours, 2 hours, 1 hour respectively each extraction time, merge extractive liquid,, filters, the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.20, adds ethanol, and making containing alcohol amount is 40%, standing 24 hours, filter, filtrate is condensed into fluid extract, and adding purified water ad pond om is 3 times of medical material gross weight, fill, obtains mixture of the present invention.
Add mixture adjuvant sucrose, ethyl ester, water, potassium sorbate to stir, make mixture.
Embodiment 4 tablets
Get panoxadiol's glycosides 5g, Radix Ophiopogonis 20g, Radix Asparagi 230g, Semen Ziziphi Spinosae 15g, Semen Platycladi 15g, Radix Codonopsis 15g, Radix Scrophulariae 15g, Fructus Corni 15g, Fructus Alpiniae Oxyphyllae 15g, Semen Cuscutae 15g.By above-mentioned whole medical materials alcohol reflux 2 times, each concentration of alcohol is respectively 80%, 60%, each ethanol consumption is respectively 10 times, 8 times of medical material total amount, extraction time is respectively 4 hours, 3 hours, and merge extractive liquid, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add microcrystalline Cellulose, cross-linking sodium carboxymethyl cellulose, micropowder silica gel, make tablet.
Embodiment 5 capsules
Get panoxadiol's glycosides 15g, Radix Ophiopogonis 40g, Radix Asparagi 40g, Semen Ziziphi Spinosae 35g, Semen Platycladi 35g, Radix Codonopsis 35g, Radix Scrophulariae 35g, Fructus Corni 35g, Fructus Alpiniae Oxyphyllae 35g, Semen Cuscutae 35g.By above-mentioned whole medical materials, extracting in water 3 times, three amount of water are respectively 12 times, 8 times, 6 times of medical material gross weight, three extraction times are respectively 3 hours, 2 hours, 1 hour, and merge extractive liquid, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add supplementary product starch, ethanol, dextrin, magnesium stearate, incapsulate shell, make capsule.
Embodiment 6 granules
Get panoxadiol's glycosides 10g, Radix Ophiopogonis 20g, Radix Asparagi 40g, Semen Ziziphi Spinosae 20g, Semen Platycladi 30g, Radix Codonopsis 35g, Radix Scrophulariae 20g, Fructus Corni 35g, Fructus Alpiniae Oxyphyllae 25g, Semen Cuscutae 35g.By above-mentioned whole medical materials, extracting in water 2 times, each amount of water is equivalent to respectively 12 times, 10 times of medical material gross weight, each extraction time is respectively 3 hours, 2 hours, and merge extractive liquid, filters, filtrate is concentrated into the clear paste that at 80 ℃, relative density is 1.05, adds ethanol, and making containing alcohol amount is 70%, standing 12 hours, filter, filtrate is condensed into after fluid extract, is dried to dried cream powder, add adjuvant Icing Sugar, starch, dextrin, ethanol, make granule.
Embodiment 7 powder
Take panoxadiol's glycosides 8g, Radix Ophiopogonis 30g, Radix Asparagi 30g, Semen Ziziphi Spinosae 20g, Semen Platycladi 15g, Radix Codonopsis 30g, Radix Scrophulariae 25g, Fructus Corni 35g, Fructus Alpiniae Oxyphyllae 25g, Semen Cuscutae 30g.Medical material cleans, pulverizes, and crosses 80-100 mesh sieve, obtains powder of the present invention.
Pharmacodynamics test: improve the memory effect method of inspection
One, step down test
1, experiment material
1.1 animal
Healthy SPF level Kunming mouse, male and female dual-purpose, body weight 20 ± 2g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
1.2 given the test agent and reagent
Embodiment of the present invention 1-5, comparative example (Piracetam Tablet), scopolamine
2 experiment groupings
80 of mices, 2 monthly age adult mices, are divided into 8 groups at random, and every group of 10 animals are respectively blank group, model group, comparative example group, embodiment 1-5 group.
3 experimental techniques
Each group of embodiment 1-5 gives test sample all by medical material crude drug amount 4.5g/kg, and every day, gavage was 1 time; Normal group and model group give 0.9% sodium chloride solution, and positive controls gives brain rehabilitation sheet by 1.2g/kg, continuously 30d.Animal is weighed weekly to twice, to adjust given the test agent dosage.Training in the 30th day.At first 10 minutes lumbar injection scopolamine normal saline solutions of training, blank group injection equivalent normal saline.Every batch of each group of experiment has a mice to test sample, operation repetitive, by that analogy.During training, first 5 mices are put into respectively to 5 grid of diving tower instrument, first conform 3 minutes, then energising, after mice is shocked by electricity, majority is jumped onto diving tower, escapes electric shock.The mice biped of take while jumping off contacts copper grid as electric shock simultaneously, is considered as wrong reaction, trains 5 minutes, and records the number of times that gets an electric shock in 5 minutes, after 24 hours, retests, and take incubation period and electric shock number of times to record test result as observation index.
4 experimental results
Table 1 is respectively organized mice step down test result
Note: with normal group comparison,
▲ ▲p < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; With the comparison of comparative example group,
#p < 0.05,
##p < 0.01.
Table 1 result shows, with model group comparison, embodiment 1-5 group and comparative example group and comparative example group mice step down test errors number obviously reduce (P<0.05, P<0.01), and there is significant difference (P<0.05, P<0.01) between embodiment 1-5 group and comparative example group.In addition, with model group comparison, embodiment 1-5 group and comparative example group obviously shorten (P<0.05 incubation period, P<0.01), and between embodiment 1-5 group and comparative example, difference has statistical significance (P<0.05, P<0.01).
Experimental result shows, embodiment 1-5 group reduces with respect to model group prolongation of latency and errors number, can think test sample to mice be improved, the effect of consolidating memory.
Two, Morris water maze test (improving memory)
1 experiment material
1.1 animal
Healthy SPF level Kunming mouse, male and female dual-purpose, body weight 20 ± 2g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
1.2 given the test agent and reagent
Embodiment of the present invention 1-5, comparative example (Piracetam Tablet), scopolamine
2 experiment groupings
80 of mices, 2 monthly age adult mices, are divided into 8 groups at random, and every group of 10 animals are respectively blank group, model group, comparative example group, embodiment 1-5 group.
3 experimental techniques
Each group of embodiment 1-5 gives test sample all by medical material 4.5g/kg, and every day, gavage was 1 time; Normal group and model group give 0.9% sodium chloride solution, and positive controls gives Gan Beier board Rhizoma Zingiberis Recens ginseng capsule, continuously 30d.Animal is weighed weekly to twice, to adjust given the test agent dosage.Within the 30th day, start training.At first 10 minutes lumbar injection scopolamine normal saline solutions of training, blank group injection equivalent normal saline.Experiment allows mice free swimming 2min to adapt to surrounding the previous day, and from first day, every batch of experiment has 1 mice to test sample, operation repetitive, by that analogy.Train every day 4 times, each random place of entry of selecting, puts into water by mice towards pool wall, and the video camera with display system is settled in top, labyrinth, and computer is from motion tracking timing record swimming track.4 times training mice enters water from four different place of entry respectively, if mice is not found platform in 120s, need be caused platform.At this moment be designated as 120s incubation period, train interval 60s at every turn, using average time of 4 times as the training achievement of every day, train continuously 5 days.Orientation navigation test, test lasts 5d, trains every day 4 times.4 times incubation period achievement meansigma methods as the same day the final result enter last statistics.Space exploration test (Spatial probe test), the 5th afternoon carries out space exploration test, remove platform, tested mice is put into water at Northwest Quadrant pool wall mid point towards pool wall, record the number of times of mice spanning platform in 60s as the Memory result of mice.
4 experimental results
Table 2 is respectively organized water maze test in mice result
Note: with normal group comparison,
▲ ▲p < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; With the comparison of comparative example group,
#p < 0.05,
##p < 0.01.
The demonstration of table 2 experimental result, compares with blank group, and obviously extend the incubation period of appearing on the stage of model group mice, and the number of times of spanning platform (being errors number) obviously increases (P < 0.01); Embodiment 1-5 group was from the 2nd day, and comparative example group is appeared on the stage and compared with model group, obviously shortened (P < 0.05, P < 0.01) since the 3rd day animal incubation period.Embodiment 1-5 group and the comparison of comparative example group, obviously shorten (P<0.05 the incubation period of embodiment 1-5 group mice, P<0.01), errors number obviously reduces (P<0.05, P<0.01).Result shows, the cognitive disorder that embodiment of the present invention 1-5 causes mice to scopolamine is improved effect.
Conclusion: give mice of the present invention, in step down test and Morris water maze test, errors number obviously reduces (P<0.05, P<0.01), obviously be better than comparative example group, and between comparative example group, there is significant difference (P<0.05, P<0.01), can think the present invention to mice be improved, the effect of consolidating memory.
Although, above used general explanation, the specific embodiment and test, the present invention is described in detail, on basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. improve a Chinese medicine composition for memory, it is characterized in that it is to be prepared from by the raw material that comprises following weight portion: panoxadiol's glycosides 5-15 part, Radix Ophiopogonis 20-40 part, Radix Asparagi 20-40 part, Semen Ziziphi Spinosae 15-35 part, Semen Platycladi 15-35 part, Radix Codonopsis 15-35 part, Radix Scrophulariae 15-35 part, Fructus Corni 15-35 part, Fructus Alpiniae Oxyphyllae 15-35 part, Semen Cuscutae 15-35 part.
2. the Chinese medicine composition that improves memory as claimed in claim 1, is characterized in that it is to be prepared from by the raw material that comprises following weight portion: panoxadiol's glycosides 8-12 part, Radix Ophiopogonis 25-35 part, Radix Asparagi 25-35 part, Semen Ziziphi Spinosae 20-30 part, Semen Platycladi 20-30 part, Radix Codonopsis 20-30 part, Radix Scrophulariae 20-30 part, Fructus Corni 20-30 part, Fructus Alpiniae Oxyphyllae 20-30 part, Semen Cuscutae 20-30 part.
3. the Chinese medicine composition that improves memory as claimed in claim 1, is characterized in that it is to be prepared from by the raw material that comprises following weight portion: 10 parts of panoxadiol's glycosides, 30 parts of Radix Ophiopogonis, 30 parts of Radix Asparagis, 25 parts of Semen Ziziphi Spinosaes, 25 parts of Semen Platycladi, 25 parts of Radix Codonopsis, 25 parts of Radix Scrophulariaes, 25 parts of Fructus Corni, 25 parts of Fructus Alpiniae Oxyphyllae, 25 parts of Semen Cuscutae.
4. the Chinese medicine composition as described in claim 1-3 any one, the active component that it is characterized in that it is to adopt the ethanol extraction of water extraction or 40-80% concentration expressed in percentage by volume to be prepared from.
5. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, and merge extractive liquid, filters, filtrate is condensed into fluid extract, obtains described active component.
6. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, merge extractive liquid,, filter, the clear paste that when filtrate is concentrated into 70-80 ℃, relative density is 1.05-1.20, adds ethanol, and making containing alcohol amount is 40-70%, standing 12-24 hour, filter, filtrate is condensed into fluid extract, obtains described active component.
7. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, with 40-80% alcohol reflux 2-3 time, the 4-10 that each ethanol consumption is medical material total amount doubly, extraction time is 1-3 hour, and merge extractive liquid, filters, filtrate is condensed into fluid extract, obtains described active component.
8. improve a Chinese medicine composition for memory, comprise acceptable carrier in the Chinese medicine composition that improves memory of any one in claim 1-3 and pharmaceuticals industry.
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| CN104172352A (en) * | 2014-08-08 | 2014-12-03 | 张会生 | Health beverage for reinforcing intelligence, invigorating brain and tranquilizing mind and preparation method of health beverage |
| CN104522680A (en) * | 2014-12-10 | 2015-04-22 | 常州诺瑞格纳米科技有限公司 | Brain-strengthening health product with effect of enhancing memory |
| CN104587211A (en) * | 2014-12-29 | 2015-05-06 | 广西梧州制药（集团）股份有限公司 | Composition for improving memory and preparation method of composition |
| CN105687564A (en) * | 2016-02-23 | 2016-06-22 | 广西梧州制药（集团）股份有限公司 | Traditional Chinese medicine composition for improving sleep and memory and preparation method of traditional Chinese medicine composition for improving sleep and memory |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104172352A (en) * | 2014-08-08 | 2014-12-03 | 张会生 | Health beverage for reinforcing intelligence, invigorating brain and tranquilizing mind and preparation method of health beverage |
| CN104172352B (en) * | 2014-08-08 | 2016-08-24 | 张会生 | Health promoting beverage that a kind of brain healthy is allayed excitement and preparation method thereof |
| CN104522680A (en) * | 2014-12-10 | 2015-04-22 | 常州诺瑞格纳米科技有限公司 | Brain-strengthening health product with effect of enhancing memory |
| CN104587211A (en) * | 2014-12-29 | 2015-05-06 | 广西梧州制药（集团）股份有限公司 | Composition for improving memory and preparation method of composition |
| CN105687564A (en) * | 2016-02-23 | 2016-06-22 | 广西梧州制药（集团）股份有限公司 | Traditional Chinese medicine composition for improving sleep and memory and preparation method of traditional Chinese medicine composition for improving sleep and memory |
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| CN103735973B (en) | 2016-05-11 |
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