CN103735973B - A kind of Chinese medicine composition that improves memory and preparation method thereof - Google Patents
A kind of Chinese medicine composition that improves memory and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine composition that improves memory and preparation method thereof. Said composition is that raw material is prepared from by medicinal materials such as panoxadiol glycosides, the tuber of dwarf lilyturf, asparagus fern, spina date seed, the seed of Oriental arborvitae, Radix Codonopsis, radix scrophulariae, Fructus Corni, fructus alpiniae oxyphyllae, the seed of Chinese dodders. The present invention also provides the preparation method of this Chinese medicine composition that improves memory. Take mouse of the present invention, in step down test and Morris water maze test, errors number obviously reduces (P<0.05, P<0.01), obviously be better than comparative example group, and between comparative example group, there is significant difference (P<0.05, P<0.01), can think the present invention to mouse be improved, the effect of consolidating memory.
Description
Technical field
The present invention relates to pharmaceutical sanitary field, be specifically related to a kind of Chinese medicine composition and preparation side thereof that improves memoryMethod.
Background technology
In man memory process, be exactly that Cerebral Cortex Neuronal Cells is actively movable, the process that records and preserve, noteRecall power relevant with the activity of brain cell on cerebral cortex. In the time carrying out mental activity, brain cell needs a large amount of oxygen.Although only 1.4 kilograms of people's brain weights, its oxygen demand accounts for 1/5～1/4 of whole body oxygen demand, is that whole body needs" rich and influential family " that oxygen amount is maximum, institute's energy requirement all will be supplied with by cell. Power work is heavy or when nervous, it is requiredOxygen and nutrient all can increase exponentially, and because brain itself can not be laid in more energy, it needs in bodyOther histocytes are supplied with energy continuously to it, can form not in time brainfag, brain if supplementedBurnout.
If brain is in burnout and sub-health state, its memory certainly will be had a greatly reduced quality. Failure of memory is brainfag[8] inevitable performance brain form. Therefore, keep the memory function that brain is good, must ensure that cerebral cortex is pure and freshActive, oxygen supply is abundant.
Obviously, strengthen providing brain with oxygen, relieving brain fatigue is the inevitable choice that improves memory.
At present, the health products that improve memory on market be mainly take in various nutriments, trace element, as DHA,Zinc, iron, magnesium etc. But, bad if the absorption of trace element is controlled, can affect the absorption of other nutriments,For example, take in too much zinc, can affect the absorption of calcium, and affect the formation of bone. Studies have shown that memory and noteMeaning power has very large association, and it is with single-hearted devotion to only have, and with breathless interest, information and object just can iron dark in cerebral cortexDark trace; Otherwise, absent minded, be not intended to note too much, can make people's decrease of memory.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine composition that improves memory;
Another object of the present invention is to provide a kind of Chinese medicine composition preparation method who improves memory.
The object of the invention is to realize by following technical scheme:
The Chinese medicine composition that improves memory of the present invention is to be prepared from by the raw material containing following weight portion:
Panoxadiol glycosides 5-15 part, the tuber of dwarf lilyturf 20-40 part, asparagus fern 20-40 part, spina date seed 15-35 part, cypressSub-benevolence 15-35 part, Radix Codonopsis 15-35 part, radix scrophulariae 15-35 part, Fructus Corni 15-35 part, fructus alpiniae oxyphyllae 15-35Part, seed of Chinese dodder 15-35 part.
Preferably, be to be prepared from by the raw material containing following weight portion:
Panoxadiol glycosides 8-12 part, the tuber of dwarf lilyturf 25-35 part, asparagus fern 25-35 part, spina date seed 20-30 part, cypressSub-benevolence 20-30 part, Radix Codonopsis 20-30 part, radix scrophulariae 20-30 part, Fructus Corni 20-30 part, fructus alpiniae oxyphyllae 20-30Part, seed of Chinese dodder 20-30 part.
More preferably, be to be prepared from by the raw material containing following weight portion:
10 parts of panoxadiol glycosides, 30 parts of the tubers of dwarf lilyturf, 30 parts of asparagus ferns, 25 parts of spina date seeds, 25 parts of the seed of Oriental arborvitaes, Radix Codonopsis25 parts, 25 parts of radix scrophulariaes, 25 parts of Fructus Cornis, 25 parts of fructus alpiniae oxyphyllaes, 25 parts of the seed of Chinese dodders.
Below crude drug source of the present invention:
PDS: the one that is ginsenoside. Ginsenoside (Ginsenoside) is a kind of steroid chemical combinationThing, triterpenoid saponin. Mainly be present in Araliaceae Panax medicinal material, Panax majority is some rare traditional Chinese medicines, asPseudo-ginseng, ginseng, American Ginseng etc. Ginsenoside can be divided into three groups of two classes according to the difference of sapogenin, and a class is fourRing triterpene Dammar methane series saponin(e, wherein one group of acid hydrolysis end product is panoxadiol, as ginsenoside Rb1,Rb2, Rb3, Rc, Rd, Rg3, Rh2 etc.
The tuber of dwarf lilyturf: be the liliaceous plant tuber of dwarf lilyturf (dwarf lilyturf) Ophiopogonjaponicus(T.f) Ker-Gawl.Dried root. Excavate summer, cleans, and is repeatedly exposed to the sun, banks up, and to dry very likely, removesFibrous root, dry. [propertyTaste] sweet micro-hardship, cold. [return through] enters lung, stomach, the heart channel of Hang-Shaoyin. [function cures mainly] Yin nourishing and lung moistening, the relieving restlessness that clears away heart-fire,Reinforcing stomach reg fluid. Control dryness of the lung dry cough, spit blood, spitting of blood, consumptive lung disease, lung carbuncle, consumptive disease dysphoria with smothery sensation, quenches one's thirst, pyreticosis Tianjin wound,Dry throat, constipation.
Asparagus fern: this product is liliaceous plant lucid asparagus Asparaguscochinchinensis(Lour.) Merr. dryPiece root. Autumn, two seasons of winter excavate, and clean, and remove stem foot and fibrous root, put and in boiling water, boil or steam to the saturating heart, remove while hotCrust, cleaning, drying. [nature and flavor] are sweet, bitter, cold. [return through] returns lung, kidney channel. [function cures mainly] yin-nourishingMoisturize, clearing lung-heat is promoted the production of body fluid. For dryness of the lung dry cough, pertussis phlegm is glutinous, dry throat and mouth, the dry constipation of intestines.
Spina date seed: this product is Rhamnaceae plant wild jujube ZiziphusjujubaMill.var.spinosa(Bunge) HuexThe dry mature seed of H.F.Chou. The time of the year when autumn changes into winter ripening fruits of gathering, removes pulp and nucleocapsid, collects seed,Dry. [nature and flavor] are sweet, sour, flat. [return through] returns liver, courage, the heart channel of Hang-Shaoyin. [function cures mainly] tonifying liver, Ning Xin,Arrest sweating, promotes the production of body fluid. For restlessness of asrhenia type and insomnia, palpitation with fear dreaminess, the empty hidrosis of body, Tianjin wound is thirsty.
The seed of Oriental arborvitae: this product is Cupressaceae plant arbor-vitae Platycladusorientalis(L.) the drying and ripening kind of FrancoBenevolence. The mature seed of gathering in autumn, two seasons of winter, dries, and removes seed coat, collects kind of a benevolence. [nature and flavor] are sweet, flat. [returnThrough] thoughts of returning home, kidney, large intestine channel. [function cures mainly] mental-tranquilization, hidroschesis, ease constipation. For dysphoria insomnia, the heartThe palpitation of throbbing with fear, night sweat due to yin deficiency, the dry constipation of intestines.
Radix Codonopsis: this product is Campanulaceae Radix Codonopsis Codonopsispilosula(Franch.) Nannf., element flower Radix Codonopsis(RADIX CODONOPSIS PILOSULAE from Northwest of China) CodonopsispilosulaNannf.var.modesta(Nannf.) L.T.Shen or radix codonpsis tangshenCodonopsistangshenOliv. dry root. Excavate autumn, cleans, and dries. [nature and flavor] are sweet, flat. [return through]Return spleen, lung channel. [function cures mainly] tonifying middle-Jiao and Qi, invigorating the spleen benefit lung. For a little less than the spleen deficiency syndrome of the lung, the palpitaition of breathing hard, food is few justHalf congealed, void is breathed with cough and is coughed, and interior heat is quenched one's thirst.
Radix scrophulariae: this product is the dry root of goatweed radix scrophulariae ScrophularianingpoensisHemsl.. Winter stemWhen leaf is withered, excavate. Remove rhizome, young shoot, fibrous root and silt, shine or be dried to half-driedly, stack 3～6 days, anti-Plural number is extremely dried. [nature and flavor] are sweet, bitter, salty, are slightly cold. [return through] returns lung, stomach, kidney channel. [function masterControl] cool blood enriching yin, purging intense heat and detonicating. For consumption of YIN caused by febrile disease, the deep red polydipsia of tongue, febrile virulent maculae, constipation is hindered in Tianjin, hectic fever due to yinLabor is coughed, hot eyes, pharyngalgia, scrofula, diphtheria, carbuncle sore tumefacting virus.
Fructus Corni: this product is the drying and ripening of Cornaceae plant Fructus Corni CornusofficinalisSieb.etZucc.Pulp. Picking fruit when time of the year when autumn changes into winter, pericarp reddened, after drying or put in boiling water and slightly to scald with slow fire, in time except removal core,Dry. [nature and flavor] acid, puckery, tepor. [return through] returns liver, kidney channel. [function cures mainly] tonifies the liver and kidney, puckeryEssence is solid de-. For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating collapse.Interior heat is quenched one's thirst.
Fructus alpiniae oxyphyllae: this product is the dry mature fruit of zingiberaceous plant intelligence development AlpiniaoxyphyllaMiq.. Summer, autumnBetween fruit gather during by green reddening, dry or low temperature drying. [nature and flavor] are pungent, temperature. [return through] returns spleen, kidney channel.[function cures mainly] is warming spleen and stopping diarrha, takes the photograph saliva, warm kidney, controlling nocturnal emission with astringent drugs contracting urine. Have loose bowels for malaria, crymodynia in abdomen, mouthfulMany salivas, enuresis with renal asthenia, frequent urination, seminal emission gonorrhoea.
The seed of Chinese dodder: this product is the dry mature seed of convolvulus cuscuta plant CuscutachinensisLam.. AutumnThe plant of gathering when fruit maturation in season, dries, and lays seed, removes impurity. [nature and flavor] are sweet, temperature. [return through]Return liver,kidney,spleen warp. [function cures mainly] is nourishing liver and kidney, and controlling nocturnal emission with astringent drugs contracting urine is antiabortive, improving eyesight, antidiarrheal. For sunImpotence seminal emission, dripping urination, enuresis frequent micturition, soreness and weakness of waist and knees, blurred vision tinnitus, vaginal bleeding due to deficiency of the kidney during pregnancy, fetal irritability, spleen kidneyVoid is rushed down; Externally treating leucoderma.
Another aspect of the present invention has been to provide the preparation method of the active component of Chinese medicine composition of the present invention, and the method isAdopt the alcohol extract of water extraction or 40-80% concentration expressed in percentage by volume. Concrete preparation method is as follows:
Scheme one: get whole medicinal materials, extracting in water 2-3 time, each amount of water is equivalent to the 6-12 of medicinal material gross weightDoubly, each extraction time is 1-3 hour, merges extract, filters, and filtrate is condensed into liquid extract, obtains described workProperty composition.
Scheme two: get whole medicinal materials, extracting in water 2-3 time, each amount of water is equivalent to the 6-12 of medicinal material gross weightDoubly, each extraction time is 1-3 hour, merges extract, filters relative density when filtrate is concentrated into 70-80 DEG CFor the clear cream of 1.05-1.20, add ethanol, making containing alcohol amount is 40-70%, leaves standstill 12-24 hour, filters filtrateBe condensed into liquid extract, obtain described active component.
Scheme three: get whole medicinal materials, with 40-80% alcohol reflux extraction 2-3 time, each ethanol consumption is medicinal material total amount4-10 doubly, extraction time is 1-3 hour, merges extract, filters, filtrate is condensed into liquid extract, obtains instituteState active component.
The Chinese medicine composition that improves memory of the present invention, can coordinate with pharmaceutically acceptable carrier, systemBecome various common dosage forms, as tablet, granule, capsule, syrup, mixture etc.
Pharmaceutically acceptable carrier of the present invention includes but not limited to following:
Diluent: starch, Icing Sugar, lactose, dextrin, microcrystalline cellulose, inorganic salts, glycitols etc.
Wetting agent and adhesive: purified water, ethanol, gelatin, polyethylene glycol, cellulose derivative etc.
Disintegrant: starch, sodium carboxymethyl starch, cellulose derivative, PVPP etc.
Lubricant: dolomol, superfine silica gel powder, talcum powder, polyethylene glycol etc.
Cosolvent: water, ethanol, glycerine, propane diols, liquid paraffin, plant wet goods.
Flavouring: sucrose, monose, aromatic etc.
Anticorrisive agent: benzoic acid, sorbic acid, methyl esters, ethyl ester, propyl ester etc.
Method 1: tablet
Get scheme one to one of scheme three gained active components, be dried to dried cream powder, add tablet to commonly use auxiliary material, by normalRule production method is prepared into tablet of the present invention.
The conventional auxiliary material of above-mentioned tablet comprises one of diluent, wetting agent, adhesive, disintegrant, lubricant or whole.
Method 2: granule
Get scheme one to one of scheme three gained active components, be dried to dried cream powder, add granule to commonly use auxiliary material, pressConventional production method is prepared into granule of the present invention.
The conventional auxiliary material of above-mentioned granule comprises one of diluent, wetting agent, adhesive, disintegrant or whole.
Method 3: capsule
Get scheme one to one of scheme three gained active components, be dried to dried cream powder, add capsule to commonly use auxiliary material, pressConventional production method is prepared into capsule of the present invention.
The conventional auxiliary material of above-mentioned capsule comprises one of diluent, wetting agent, adhesive, disintegrant, lubricant or whole.
Method 4: syrup
Get scheme one to one of scheme three gained active components, add syrup to commonly use auxiliary material, routinely producer legal systemFor obtaining syrup of the present invention.
The conventional auxiliary material of above-mentioned syrup comprises one of flavouring, anticorrisive agent, cosolvent or whole.
Method 5: mixture
Get scheme one to one of scheme three gained active components, add mixture to commonly use auxiliary material, production method preparation routinelyObtain mixture of the present invention.
The conventional auxiliary material of above-mentioned mixture comprises one of flavouring, anticorrisive agent or whole.
Fang Xie: we's card is the card of breakdown of the normal physiological coordination between the heart and the kidney, the deficiency of YIN-blood of the heart, the mind is lost and is supported, holds concurrently and the deficiency of the kidney yin, brainInanition, so kidney water can not on help in the heart, internal heat can not meet at down kidney. Demonstrate,prove by pondering over too strain heart kidney, cloudy bloodDark consumption, due to extreme misery can not help mutually, clinical condition is shown in vexed insomnia, forgetful nocturnal emission. Control suitable enriching yin liquid, clearind deficient heat, supportsThe mind. We monarch is with two winters, and the tuber of dwarf lilyturf can thoughts of returning home warp, nourishes heart the moon and the heat that clears away heart-fire; Asparagus fern can be returned kidney channel, nourishing kidney-yin and fallingThe fire of deficiency type, the two medicines nutrient solution of promoting the production of body fluid again, strong water fire processed. The product of spina date seed, all mental-tranquilizations of the seed of Oriental arborvitae, spina date seed nourish heart the moon,Benefit liver blood and calming the nerves, seed of Oriental arborvitae taste Gan Zhirun, can nourish again cloudy liquid, and principal drug assistance is strengthened water fire processed; Radix Codonopsis is enriched blood and is promoted the production of body fluid,Cloudy blood in the heart has help; Radix scrophulariae is nourishing Yin and clearing heat, can help again asparagus fern to fall fire mutually, is all ministerial drug. Fructus Corni, fructus alpiniae oxyphyllae,The seed of Chinese dodder all can tonifying kidney and strengthening yang, and Fructus Corni is held concurrently can benefit essence, and it is intended to " treating YIN within YANG, cloudy positive liter, and source is without cease ".All medicines share, play altogether nourishing Yin and clearing heat, the merit of mental-tranquilization.
The present invention adds panoxadiol glycosides in above-mentioned side. Panoxadiol glycosides has the effect that improves ability of learning and memory,This main and panoxadiol glycosides improve brain blood and supply relevantly, and it is also that it improves large cerebrology to the removing of oxygen radicalPractise one of mechanism of memory capability. Above-mentioned side assists with panoxadiol glycosides, stronger to the improvement effect of brain memory ability.
A kind of Chinese medicine composition that improves memory provided by the invention has the following advantages:
1, the present invention has nourishing Yin and clearing heatly, and effect of nourishing yin and supplementing blood, mental-tranquilization, can improve people's noticeAnd memory. Give mouse step down test errors number of the present invention and obviously reduce (P < 0.01), and the present invention and contrastBetween example group, there is significant difference (P < 0.05, P < 0.01). The present invention subtracts for model group prolongation of latency and errors numberFew, can think test sample to mouse be improved, the effect of consolidating memory.
2,, in Morris water maze test, compared with comparative example group, give mouse of the present invention and obviously contract incubation periodShort (P < 0.05, P < 0.01), errors number obviously reduces (P < 0.05, P < 0.01). Result shows, the present inventionThe cognitive disorder that hyoscine is caused to mouse is improved effect.
3, Chinese traditional medicine composition composition formula of the present invention is rationally innovated, safe and effective, and production cost is low, technique is simple.
Detailed description of the invention
Further illustrate the present invention below by embodiment. It should be understood that embodiments of the invention are for explanationThe present invention instead of limitation of the present invention. The simple modifications that essence according to the present invention is carried out the present invention all belongs to thisInvent claimed scope.
Embodiment 1 syrup
Get panoxadiol glycosides 10g, tuber of dwarf lilyturf 30g, asparagus fern 30g, spina date seed 25g, seed of Oriental arborvitae 25g, Radix Codonopsis 25g,Radix scrophulariae 25g, Fructus Corni 25g, fructus alpiniae oxyphyllae 25g, seed of Chinese dodder 25g. By above-mentioned whole medicinal materials, extracting in water 2 times,Twice amount of water is respectively 10 times, 8 times of medicinal material gross weight, and the extracted twice time is respectively 2 hours, 1 hour,Merge extract, filter, filtrate is condensed into liquid extract. In medicinal extract, add syrup auxiliary material sucrose, honey, sorbAcid potassium, water stir, and make syrup.
Embodiment 2 tablets
Get panoxadiol glycosides 8g, tuber of dwarf lilyturf 25g, asparagus fern 25g, spina date seed 20g, seed of Oriental arborvitae 20g, Radix Codonopsis 20g, profoundGinseng 20g, Fructus Corni 20g, fructus alpiniae oxyphyllae 20g, seed of Chinese dodder 20g. By above-mentioned whole medicinal materials, carry with 40% alcohol refluxGet 3 times, each ethanol consumption is respectively 10 times, 8 times, 4 times of medicinal material total amount, 3 hours respectively extraction time,2 hours, 1 hour, merge extract, to filter, filtrate is condensed into after liquid extract, is dried to dried cream powder, adds auxiliaryMaterial starch, ethanol, talcum powder, make tablet.
Embodiment 3 mixture
Get panoxadiol glycosides 12g, tuber of dwarf lilyturf 35g, asparagus fern 35g, spina date seed 30g, seed of Oriental arborvitae 30g, Radix Codonopsis 30g,Radix scrophulariae 30g, Fructus Corni 30g, fructus alpiniae oxyphyllae 30g, seed of Chinese dodder 30g. By above-mentioned whole medicinal materials, extracting in water 3 times,Three amount of water are equivalent to respectively 12 times, 8 times, 6 times of medicinal material gross weight, 3 hours respectively each extraction time,2 hours, 1 hour, merge extract, filter, the clear cream that when filtrate is concentrated into 70 DEG C, relative density is 1.20, addsEnter ethanol, making containing alcohol amount is 40%, leaves standstill 24 hours, filters, and filtrate is condensed into liquid extract, adds purified water to totalWeight is 3 times of medicinal material gross weight, filling, obtains mixture of the present invention.
Add mixture auxiliary material sucrose, ethyl ester, water, potassium sorbate to stir, make mixture.
Embodiment 4 tablets
Get panoxadiol glycosides 5g, tuber of dwarf lilyturf 20g, asparagus fern 230g, spina date seed 15g, seed of Oriental arborvitae 15g, Radix Codonopsis 15g,Radix scrophulariae 15g, Fructus Corni 15g, fructus alpiniae oxyphyllae 15g, seed of Chinese dodder 15g. Above-mentioned whole medicinal materials are extracted to 2 with alcohol refluxInferior, each concentration of alcohol is respectively 80%, 60%, and each ethanol consumption is respectively 10 times, 8 times of medicinal material total amount,Extraction time is respectively 4 hours, 3 hours, merges extract, filters, and filtrate is condensed into after liquid extract, is dried toDried cream powder, adds microcrystalline cellulose, Ac-Di-Sol, superfine silica gel powder, makes tablet.
Embodiment 5 capsules
Get panoxadiol glycosides 15g, tuber of dwarf lilyturf 40g, asparagus fern 40g, spina date seed 35g, seed of Oriental arborvitae 35g, Radix Codonopsis 35g,Radix scrophulariae 35g, Fructus Corni 35g, fructus alpiniae oxyphyllae 35g, seed of Chinese dodder 35g. By above-mentioned whole medicinal materials, extracting in water 3 times,Three amount of water are respectively 12 times, 8 times, 6 times of medicinal material gross weight, and three extraction times are respectively 3 hours, 2Hour, 1 hour, merge extract, filter, filtrate is condensed into after liquid extract, is dried to dried cream powder, adds auxiliary materialStarch, ethanol, dextrin, dolomol, incapsulate shell, make capsule.
Embodiment 6 granules
Get panoxadiol glycosides 10g, tuber of dwarf lilyturf 20g, asparagus fern 40g, spina date seed 20g, seed of Oriental arborvitae 30g, Radix Codonopsis 35g,Radix scrophulariae 20g, Fructus Corni 35g, fructus alpiniae oxyphyllae 25g, seed of Chinese dodder 35g. By above-mentioned whole medicinal materials, extracting in water 2 times,Each amount of water is equivalent to respectively 12 times, 10 times of medicinal material gross weight, and each extraction time is respectively 3 hours, 2Hour, merge extract, filter, filtrate is concentrated into the clear cream that at 80 DEG C, relative density is 1.05, adds ethanol,Making containing alcohol amount is 70%, leaves standstill 12 hours, filters, and filtrate is condensed into after liquid extract, is dried to dried cream powder, adds auxiliaryMaterial Icing Sugar, starch, dextrin, ethanol, make granule.
Embodiment 7 pulvis
Take panoxadiol glycosides 8g, tuber of dwarf lilyturf 30g, asparagus fern 30g, spina date seed 20g, seed of Oriental arborvitae 15g, Radix Codonopsis 30g,Radix scrophulariae 25g, Fructus Corni 35g, fructus alpiniae oxyphyllae 25g, seed of Chinese dodder 30g. Medicinal material cleans, pulverizes, and crosses 80-100 mesh sieve,Obtain pulvis of the present invention.
Pharmacodynamics test: improve the memory effect method of inspection
One, step down test
1, experiment material
1.1 animal
Healthy SPF level Kunming mouse, male and female dual-purpose, body weight 20 ± 2g, buys from Guangxi Medical University animal used as testCenter (production licence number: SCXK osmanthus 2009-0002).
1.2 given the test agent and reagent
Embodiment of the present invention 1-5, comparative example (Piracetam Tablet), hyoscine
2 experiment groupings
80 of mouse, 2 monthly age adult mices, are divided into 8 groups at random, every group of 10 animals, be respectively blank group,Model group, comparative example group, embodiment 1-5 group.
3 experimental techniques
Each group of embodiment 1-5 gives test sample all by medicinal material crude drug amount 4.5g/kg, every day gavage 1 time; Normal groupGive 0.9% sodium chloride solution with model group, positive controls gives brain rehabilitation sheet by 1.2g/kg, continuously 30d. WillAnimal weighs weekly twice, to adjust given the test agent dosage. Training in the 30th day. In the front 10 points of lumbar injection east of trainingHyoscyamine normal saline solution, blank group injection equivalent physiological saline. Testing each group for every batch has a mouse to test sample,Operation repetitive, by that analogy. When training, first 5 mouse are put into respectively to 5 grid of diving tower instrument, firstConform 3 minutes, then energising, after mouse is shocked by electricity, majority is jumped onto diving tower, escapes electric shock. While jumping off with littleMouse biped contacts copper grid for getting an electric shock simultaneously, is considered as wrong reaction, and train 5 minutes, and record the number of times that gets an electric shock in 5 minutes,After 24 hours, retest, record test result taking incubation period and electric shock number of times as observation index.
4 experimental results
The each group of table 1 mouse step down test result
Note: with normal group comparison,▲▲P < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; WithThe comparison of comparative example group,#P＜0.05，##P＜0.01。
The demonstration of table 1 result, with model group comparison, embodiment 1-5 group and comparative example group and the examination of comparative example group mouse diving towerTest errors number and obviously reduce (P < 0.05, P < 0.01), and have significant difference between embodiment 1-5 group and comparative example group(P < 0.05, P < 0.01). In addition,, with model group comparison, embodiment 1-5 group and comparative example group obviously shorten incubation period(P < 0.05, P < 0.01), and between embodiment 1-5 group and comparative example, difference has statistical significance (P < 0.05, P < 0.01).
Experimental result shows, embodiment 1-5 group reduces with respect to model group prolongation of latency and errors number, can recognizeFor test sample to mouse be improved, the effect of consolidating memory.
Two, Morris water maze test (improving memory)
1 experiment material
1.1 animal
Healthy SPF level Kunming mouse, male and female dual-purpose, body weight 20 ± 2g, buys from Guangxi Medical University animal used as testCenter (production licence number: SCXK osmanthus 2009-0002).
1.2 given the test agent and reagent
Embodiment of the present invention 1-5, comparative example (Piracetam Tablet), hyoscine
2 experiment groupings
80 of mouse, 2 monthly age adult mices, are divided into 8 groups at random, every group of 10 animals, be respectively blank group,Model group, comparative example group, embodiment 1-5 group.
3 experimental techniques
Each group of embodiment 1-5 gives test sample all by medicinal material 4.5g/kg, every day gavage 1 time; Normal group and modelGroup gives 0.9% sodium chloride solution, and positive controls gives Gan Beier board ginger ginseng capsule, continuously 30d. By animal weeklyWeigh twice, to adjust given the test agent dosage. Within the 30th day, start training. The front 10 points of lumbar injection Anisodus luridus of trainingAlkali normal saline solution, blank group injection equivalent physiological saline. Experiment allows mouse free swimming 2min to fit the previous dayAnswer surrounding environment, from first day, every batch of experiment has 1 mouse to test sample, operation repetitive, by that analogy.Train every day 4 times, each random place of entry of selecting, puts into water by mouse towards pool wall, and settle top, labyrinthWith the video camera of display system, computer is from motion tracking timing record swimming track. 4 times training mouse respectively fromFour different place of entry enter water, if mouse is not found platform in 120s, need be caused platform. At this moment diveThe volt phase is designated as 120s, trains interval 60s at every turn, the training achievement using the average times of 4 times as every day, continuously instructionPractice 5 days. Orientation navigation test, test lasts 5d, trains every day 4 times. 4 times incubation period achievement mean value conductThe same day, the final result entered last statistics. Space exploration test (Spatialprobetest), the 5th afternoon carries out spaceExploratory experiment, removes platform, and tested mouse is put into water at Northwest Quadrant pool wall mid point towards pool wall, records 60sThe number of times of interior mouse spanning platform is as the Memory result of mouse.
4 experimental results
The each group of table 2 water maze test in mice result
Note: with normal group comparison,▲▲P < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; WithThe comparison of comparative example group,#P＜0.05，##P＜0.01。
The demonstration of table 2 experimental result, compared with blank group, obviously extend the incubation period of appearing on the stage of model group mouse, passes through flatThe number of times (being errors number) of platform obviously increases (P < 0.01); Embodiment 1-5 group is from the 2nd day, comparative example group fromWithin the 3rd day, beginning animal is appeared on the stage and obviously shortens (P < 0.05, P < 0.01) compared with model group incubation period. Embodiment 1-5 groupWith the comparison of comparative example group, obviously shorten (P < 0.05, P < 0.01), mistake time the incubation period of embodiment 1-5 group mouseNumber obviously reduces (P < 0.05, P < 0.01). Result shows, embodiment of the present invention 1-5 causes mouse to hyoscineCognitive disorder is improved effect.
Conclusion: give mouse of the present invention, in step down test and Morris water maze test, errors number obviously subtractsFew (P < 0.05, P < 0.01), is obviously being better than comparative example group, and have significant difference between comparative example group (P < 0.05,P < 0.01), can think the present invention to mouse be improved, the effect of consolidating memory.
Although, above use general explanation, detailed description of the invention and test, the present invention has been done to detailed retouchingState, but on basis of the present invention, can make some modifications or improvements it, this is aobvious to those skilled in the artAnd easily see. Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to thisBright claimed scope.
Claims (8)
1. improve a Chinese medicine composition for memory, it is characterized in that it is to be prepared from by the raw material of following weight portion: panoxadiol glycosides 5-15 part, the tuber of dwarf lilyturf 20-40 part, asparagus fern 20-40 part, spina date seed 15-35 part, seed of Oriental arborvitae 15-35 part, Radix Codonopsis 15-35 part, radix scrophulariae 15-35 part, Fructus Corni 15-35 part, fructus alpiniae oxyphyllae 15-35 part, seed of Chinese dodder 15-35 part.
2. the Chinese medicine composition that improves memory as claimed in claim 1, is characterized in that it is to be prepared from by the raw material of following weight portion: panoxadiol glycosides 8-12 part, the tuber of dwarf lilyturf 25-35 part, asparagus fern 25-35 part, spina date seed 20-30 part, seed of Oriental arborvitae 20-30 part, Radix Codonopsis 20-30 part, radix scrophulariae 20-30 part, Fructus Corni 20-30 part, fructus alpiniae oxyphyllae 20-30 part, seed of Chinese dodder 20-30 part.
3. the Chinese medicine composition that improves memory as claimed in claim 1, is characterized in that it is to be prepared from by the raw material of following weight portion: 10 parts of panoxadiol glycosides, 30 parts of the tubers of dwarf lilyturf, 30 parts of asparagus ferns, 25 parts of spina date seeds, 25 parts of the seed of Oriental arborvitaes, 25 parts of Radix Codonopsis, 25 parts of radix scrophulariaes, 25 parts of Fructus Cornis, 25 parts of fructus alpiniae oxyphyllaes, 25 parts of the seed of Chinese dodders.
4. the Chinese medicine composition as described in claim 1-3 any one, the active component that it is characterized in that it is to adopt the alcohol extract of water extraction or 40-80% concentration expressed in percentage by volume to be prepared from.
5. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medicinal materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medicinal material gross weight, each extraction time is 1-3 hour, merges extract, filters, filtrate is condensed into liquid extract, obtains described active component.
6. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medicinal materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medicinal material gross weight, each extraction time is 1-3 hour, merge extract, filter, the clear cream that when filtrate is concentrated into 70-80 DEG C, relative density is 1.05-1.20, adds ethanol, and making containing alcohol amount is 40-70%, leave standstill 12-24 hour, filter, filtrate is condensed into liquid extract, obtains described active component.
7. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medicinal materials, with 40-80% alcohol reflux extraction 2-3 time, the 4-10 that each ethanol consumption is medicinal material total amount doubly, extraction time is 1-3 hour, merges extract, filters, filtrate is condensed into liquid extract, obtains described active component.
8. improve a Chinese medicine composition for memory, prepare and form by acceptable carrier in the Chinese medicine composition that improves memory of any one in claim 1-3 and pharmaceuticals industry.
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| CN104172352B (en) * | 2014-08-08 | 2016-08-24 | 张会生 | Health promoting beverage that a kind of brain healthy is allayed excitement and preparation method thereof |
| CN104522680A (en) * | 2014-12-10 | 2015-04-22 | 常州诺瑞格纳米科技有限公司 | Brain-strengthening health product with effect of enhancing memory |
| CN104587211A (en) * | 2014-12-29 | 2015-05-06 | 广西梧州制药（集团）股份有限公司 | Composition for improving memory and preparation method of composition |
| CN105687564A (en) * | 2016-02-23 | 2016-06-22 | 广西梧州制药（集团）股份有限公司 | Traditional Chinese medicine composition for improving sleep and memory and preparation method of traditional Chinese medicine composition for improving sleep and memory |
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| CN101011537A (en) * | 2006-12-17 | 2007-08-08 | 杨绪民 | Zhuangyuanxing pill for treating insomnia, amnesia and improving intelligence |
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| CN101011537A (en) * | 2006-12-17 | 2007-08-08 | 杨绪民 | Zhuangyuanxing pill for treating insomnia, amnesia and improving intelligence |
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| 247首安神方剂用药组方规律分析;包祖晓等;《中医杂志》;20071117(第11期);第1013-1015页 * |
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