CN106177119A - A kind of Fructus Schisandrae Chinensis granule and preparation method thereof - Google Patents
A kind of Fructus Schisandrae Chinensis granule and preparation method thereof Download PDFInfo
- Publication number
- CN106177119A CN106177119A CN201610566962.2A CN201610566962A CN106177119A CN 106177119 A CN106177119 A CN 106177119A CN 201610566962 A CN201610566962 A CN 201610566962A CN 106177119 A CN106177119 A CN 106177119A
- Authority
- CN
- China
- Prior art keywords
- fructus schisandrae
- schisandrae chinensis
- casing
- granule
- tank body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/0011—Heating features
- B01D1/0017—Use of electrical or wave energy
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/0082—Regulation; Control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/14—Evaporating with heated gases or vapours or liquids in contact with the liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/26—Multiple-effect evaporating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/30—Accessories for evaporators ; Constructional details thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention provides a kind of Fructus Schisandrae Chinensis granule and extracting method thereof, take Fructus Schisandrae Chinensis, add the water of 10 times of weight of schisandra chinensis medicinal material, soak 0.5h, decoct 1 hour, filtering, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, the steps such as medicinal liquid leaches, and using distinctive concentration and drying equipment, in the Fructus Schisandrae Chinensis granule prepared, schisandrin content is high, and it was found that the application in preparation suppression Chinese hamster ovary Chinese hamster ovary celI hyperproliferation agent of the Fructus Schisandrae Chinensis granule.
Description
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of Fructus Schisandrae Chinensis granule and preparation method thereof.
Background technology
Fructus Schisandrae Chinensis granule energy supplementing QI for promoting the production of body fluid, kidney calming.For the insomnia caused by heart kidney deficiency, dreaminess, dizziness；Nerve declines
Weak disease sees above-mentioned patient.Preparation method of the prior art, technique is coarse, backward, and impurity is many, and active constituent content is low, leads
Cause patient's consumption excessive, it has not been convenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problems referred to above, it is an object of the invention to provide a kind of Fructus Schisandrae Chinensis granule and preparation thereof
Method.
Technical scheme: it is an object of the invention to by following scheme realization:
A kind of Fructus Schisandrae Chinensis granule, preparation method is: take Fructus Schisandrae Chinensis, adds the water of 10 times of weight of schisandra chinensis medicinal material, soaks 0.5h,
Decocting 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, dense
Being reduced to 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, and stirring, standing, filtration, filtrate is dense
Being reduced to relative density when 65 DEG C is 1.25, and reclaims ethanol, obtains concentrated solution, uses silica gel to refine, in advance by selected silica gel dress
Enter in resin column, after being diluted by purified for described concentrated solution water, by reverse phase silica gel post, remove the impurity in liquid simultaneously, receive
Collecting whole effluent, continue to concentrate, be dried by concentrated solution, pelletize, fill obtains Fructus Schisandrae Chinensis granule.
Described a kind of Fructus Schisandrae Chinensis granule, silica gel specific surface described in preparation method is 300～500m2/g, and pore volume is 0.70
～0.90ml/g, after described dilution, concentrated solution is by the separation of reverse phase silica gel post, eluting, to only going out water colorless.
Described Fructus Schisandrae Chinensis granule, concentrates for three times in above-mentioned preparation method and uses device, including tank body, be arranged on described tank body
On charging aperture and be arranged on the discharge nozzle of described tank base, described discharge nozzle is provided with discharge valve and filter,
It is provided with shaft in described tank body, described shaft is provided with stirring paddle, be provided with and described stirring at the top of described tank body
The stirring motor that axle is connected, the diapire in described tank body is provided with vibrations chamber, is provided with jolting plate at described vibrations intracavity, in institute
State and be provided with electromagnetic shaker between jolting plate and described vibrations chamber, the sidewall in described tank body is provided with cavity, at described jolting plate
End face be provided with scraper plate, between described scraper plate and described cavity, be provided with telescopic cylinder, described scraper plate is at described telescopic cylinder
Effect is lower realizes the scraping to jolting plate surface, is provided with the discharging being connected with described discharge nozzle at the center of described jolting plate
Mouthful.
Described Fructus Schisandrae Chinensis granule, in above-mentioned preparation method, jolting plate described in enrichment facility sets with the junction in described vibrations chamber
There is sealing metal bar, described tank body is provided with the shake control device being connected with described electromagnetic shaker.
Described Fructus Schisandrae Chinensis granule, in above-mentioned preparation method, the bottom of scraper plate described in enrichment facility is provided with metal scraping layer, institute
State metal scraping layer to be pressed on described jolting plate, described tank body is provided with the extension and contraction control being connected with described telescopic cylinder
Button.
Described Fructus Schisandrae Chinensis granule, is provided with water tester in tank body described in enrichment facility in above-mentioned preparation method, described
Cooler it is provided with in discharge nozzle.
Described Fructus Schisandrae Chinensis granule, is dried in above-mentioned preparation method and uses device, including casing, be arranged in described casing
Material frame and the blast pipe and the discharge pipe that are arranged on described casing top, it is characterised in that: it is provided with in the sidewall of described casing
The air intake passage being connected with described blast pipe, is provided with the wind being connected with described air intake passage on the sidewall of described casing
Hole, described discharge pipe through described casing roof connection casing in casing outside, be additionally provided with in the sidewall of described casing
Homoiothermic chamber and sterilization chamber, be provided with electrical heating block at described homoiothermic intracavity, and described air intake passage passes described regulation chamber and sterilization chamber
Arrange, in the diapire of described casing, be provided with rotating drum, in described rotating drum, be provided with electric rotating machine, the master of described electric rotating machine
Axle is connected with described material frame, is provided with vibrations groove in described material frame, and the end face at described material frame is provided with and holds plate,
It is provided with shaking device between plate and described vibrations groove described holding.
Described Fructus Schisandrae Chinensis granule, is dried in above-mentioned preparation method and uses shaking device described in device to include driving motor, if
Put the eccentric on described driving electric machine main shaft, the wheel limit of described eccentric be pressed on described in hold the bottom center of plate,
Being provided with extension spring between the diapire of the described bottom holding plate and described vibrations groove, described extension spring is right centered by described eccentric
Claim to arrange.
Described Fructus Schisandrae Chinensis granule, is dried in above-mentioned preparation method in using casing described in device and is provided with temperature sensor,
The driver being connected with described temperature sensor, described driver and described electrical heating block it is provided with in the sidewall of described casing
Control end to be connected, described casing is additionally provided with chamber door.
The application in preparation suppression Chinese hamster ovary Chinese hamster ovary celI hyperproliferation agent of the described Fructus Schisandrae Chinensis granule.
In prior art, Fructus Schisandrae Chinensis granule uses the method that water puies forward, and technique is coarse, backward, and impurity is many, causes patient's consumption
Excessive, it has not been convenient to take, Fructus Schisandrae Chinensis granule schisandrin yield prepared by the present invention increases, and content is high.Dense in the present invention
Contracting device structure is simple, simple operation, it is provided with jolting plate and electromagnetic shaker, and is additionally provided with scraper plate and is connected with described scraper plate
Telescopic cylinder, can effectively prevent tank base from luming, be greatly improved the efficiency of heating surface to a certain extent, efficiently solves tradition skill
Caking bottom easily occurring in art Chinese medicinal concentration equipment and affect the technical deficiency of the efficiency of heating surface, stability in use is good and the suitability
By force, the stable effective ingredients of medicine is not destroyed.This drying equipment structure is simple, simple and quick, and it uses wall box
Blow, and be additionally arranged electrical heating block, can be adjusted according to the temperature in case, thus ensure that stablizing of baking temperature
Property, and it is provided with electric rotating machine and shaking device, can rotate and shake holding plate while heating, thus can prevent
Only medicine local desiccation, thus dry efficiency can be improved to a certain extent, the drying device solving conventional art is dried effect
The technical deficiency that rate is low.And find Fructus Schisandrae Chinensis granule answering in preparation suppression Chinese hamster ovary Chinese hamster ovary celI hyperproliferation agent
With.
Accompanying drawing explanation
In order to make present disclosure be more likely to be clearly understood, below according to specific embodiment and combine accompanying drawing, right
The present invention is described in further detail, wherein:
Fig. 1 is the structural representation of enrichment facility of the present invention.
Fig. 2 is the structural representation of drying device of the present invention.
Detailed description of the invention
Form by the following examples, is described in further detail the foregoing of the present invention again, but should be by this
Being interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to Examples below, all technology realized based on foregoing of the present invention are equal
Belong to the scope of the present invention.
Embodiment 1
Taking Fructus Schisandrae Chinensis 1000g, add the water of 10 times of weight of schisandra chinensis medicinal material, soak 0.5h, decoct 1 hour, filter, medicinal residues are again
Adding the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, and merges twice decoction liquor, is concentrated into 60 DEG C of relative densities and is
1.05, add ethanol and make the percent by volume of alcohol content reach 75%, stir, stand, filter, relative density when filtrate is concentrated into 65 DEG C
It is 1.25, and reclaims ethanol, obtain concentrated solution, use silica gel to refine, obtain effluent, continue to concentrate, concentrated solution is dried, system
Grain, fill obtains Fructus Schisandrae Chinensis granule, every bag of 10g.
The preparation method of described Fructus Schisandrae Chinensis granule, described silica gel process for purification is: in advance selected silica gel is loaded resin
In post, after being diluted by purified for described concentrated solution water, by reverse phase silica gel post simultaneously, remove the impurity in liquid, collect all
Effluent.
The preparation method of described Fructus Schisandrae Chinensis granule, described silica gel specific surface is 300m2/ g, pore volume is 0.90ml/g, described
After dilution, concentrated solution is by the separation of reverse phase silica gel post, eluting, to only going out water colorless.
See Fig. 1: the preparation method of described Fructus Schisandrae Chinensis granule, concentrate for above-mentioned three times and use device, including tank body 1, be arranged on
Charging aperture on described tank body 12 and be arranged on the discharge nozzle 3 bottom described tank body 1, is provided with outlet valve on described discharge nozzle 3
Door 4 and filter 5, be provided with shaft 6 in described tank body 1, be provided with stirring paddle 7 on described shaft 6, at described tank body 1
Top be provided with the stirring motor 8 being connected with described shaft 6, the diapire in described tank body 1 be provided with vibrations chamber 9, in institute
It is provided with jolting plate 10 in stating vibrations chamber 9, between described jolting plate 10 and described vibrations chamber 9, is provided with electromagnetic shaker 11, by vibrations
Device can make jolting plate shake, thus can effectively prevent drug particles from concentrating on jolting plate, thus can prevent drug particles from existing
Luming on jolting plate, the sidewall in described tank body 1 is provided with cavity 12, the end face at described jolting plate 10 is provided with scraper plate 13,
Between described scraper plate 13 and described cavity 12, it is provided with telescopic cylinder 14, telescopic cylinder can be controlled as required, make scraper plate exist
The surface of jolting plate scrapes, thus the drug particles that part concentrates at jolting plate surface scrapes, and described scraper plate 13 is stretched described
The effect of contracting cylinder 14 is lower realizes the scraping to jolting plate 10 surface, is provided with and described discharge nozzle at the center of described jolting plate 10
The discharging opening 15 that 3 are connected.
It is provided with sealing metal bar 16 in the junction of described jolting plate 10 with described vibrations chamber 9, described tank body is provided with
The shake control device 17 being connected with described electromagnetic shaker.
Be provided with metal scraping layer 18 in the bottom of described scraper plate, described metal scraping layer is pressed on described jolting plate,
Described tank body is provided with the extension and contraction control button 19 being connected with described telescopic cylinder.
In described tank body, it is provided with water tester 21, in described discharge nozzle, is provided with cooler 20.
See Fig. 2: the preparation method of described Fructus Schisandrae Chinensis granule, above-mentioned dry use device, including casing 1, be arranged on described
Material frame 2 in casing 1 and the blast pipe 3 and the discharge pipe 4 that are arranged on described casing 1 top, set in the sidewall of described casing 1
There is the air intake passage 5 being connected with described blast pipe 3, the sidewall of described casing 1 is provided with and is connected with described air intake passage 5
Air holes 6, fresh air inlet is arranged on sidewall, it is ensured that the uniformity of ventilation, and can be greatly improved and the contacting of medicine
Area, described discharge pipe 4 is through outside, in the sidewall of described casing 1 with casing 1 in the roof connection casing 1 of described casing 1
Being additionally provided with homoiothermic chamber 7 and sterilization chamber 8, be provided with electrical heating block 9 in described homoiothermic chamber 7, being provided with electrical heating block can be as required
Being adjusted the temperature of blast pipe, it is ensured that the stability of baking temperature, described air intake passage 5 is through described regulation chamber 7 and goes out
Bacterium chamber 8 is arranged, and is provided with rotating drum 10 in the diapire of described casing 1, is provided with electric rotating machine 11 in described rotating drum 10, described
The main shaft of electric rotating machine 11 is connected with described material frame 2, is provided with vibrations groove 12, at described material in described material frame 2
The end face of frame 2 is provided with and holds plate 13, is provided with shaking device 14 described holding between plate 13 and described vibrations groove 12.
Described shaking device 14 includes driving motor 141, is arranged on the eccentric 142 on described driving motor 141 main shaft,
The wheel limit of described eccentric 142 holds the bottom center of plate 13 described in being pressed on, in the described bottom holding plate 13 and described shake
Being provided with extension spring 15 between the diapire of dynamic groove 12, described extension spring is symmetrical arranged centered by described eccentric, passes through in use
Compressing of eccentric makes to hold plate rising, when the recess of eccentric compresses and holds plate, holds plate and resets under the effect of extension spring,
Make the disengaging of thing material fall again after holding plate in dropping process and hold the vibrations realizing material on plate.
In described casing 1, it is provided with temperature sensor 16, is provided with in the sidewall of described casing and described temperature sensor
The driver 17 being connected, described driver is connected with the control end of described electrical heating block.Temperature passes dry device and driver is
Existing prior art on market, it has only to when using and connecting connect according to corresponding connection description.
Described casing is additionally provided with chamber door 18.
Embodiment 2
Taking Fructus Schisandrae Chinensis 1000g, add the water of 10 times of weight of schisandra chinensis medicinal material, soak 0.5h, decoct 1 hour, filter, medicinal residues are again
Adding the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, and merges twice decoction liquor, is concentrated into 60 DEG C of relative densities and is
1.05, add ethanol and make the percent by volume of alcohol content reach 75%, stir, stand, filter, relative density when filtrate is concentrated into 65 DEG C
It is 1.25, and reclaims ethanol, obtain concentrated solution, use silica gel to refine, obtain effluent, continue to concentrate, concentrated solution is dried, system
Grain, fill obtains Fructus Schisandrae Chinensis granule, every bag of 10g.
The preparation method of described Fructus Schisandrae Chinensis granule, described silica gel process for purification is: in advance selected silica gel is loaded resin
In post, after being diluted by purified for described concentrated solution water, by reverse phase silica gel post simultaneously, remove the impurity in liquid, collect all
Effluent.
The preparation method of described a kind of Fructus Schisandrae Chinensis granule, described silica gel specific surface is 500m2/g, and pore volume is 0.70ml/g,
After described dilution, concentrated solution is by the separation of reverse phase silica gel post, eluting, to only going out water colorless.
Concentrate with drying equipment ibid.
Embodiment 3
Taking Fructus Schisandrae Chinensis 1000g, add the water of 10 times of weight of schisandra chinensis medicinal material, soak 0.5h, decoct 1 hour, filter, medicinal residues are again
Adding the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, and merges twice decoction liquor, is concentrated into 60 DEG C of relative densities and is
1.05, add ethanol and make the percent by volume of alcohol content reach 75%, stir, stand, filter, relative density when filtrate is concentrated into 65 DEG C
It is 1.25, and reclaims ethanol, obtain concentrated solution, use silica gel to refine, obtain effluent, continue to concentrate, concentrated solution is dried, system
Grain, fill obtains Fructus Schisandrae Chinensis granule, every bag of 10g.
The preparation method of described Fructus Schisandrae Chinensis granule, described silica gel process for purification is: in advance selected silica gel is loaded resin
In post, after being diluted by purified for described concentrated solution water, by reverse phase silica gel post simultaneously, remove the impurity in liquid, collect all
Effluent.
The preparation method of described a kind of Fructus Schisandrae Chinensis granule, described silica gel specific surface is 400m2/g, and pore volume is 0.80ml/g,
After described dilution, concentrated solution is by the separation of reverse phase silica gel post, eluting, to only going out water colorless.
Concentrate with drying equipment ibid.
In above-described embodiment, Fructus Schisandrae Chinensis is magnoliaceae schisandra Schisandra chinensis (Turcz.)
Baill. dry mature fruit.
Embodiment 4: schisandrin assay experimentation data in Fructus Schisandrae Chinensis granule of the present invention
1.1 Experimental agents: Fructus Schisandrae Chinensis granule of the present invention: prepare by embodiment 1-3 method.Matched group use common concentrate and
Drying means prepares Fructus Schisandrae Chinensis granule.
1.2 instruments: high performance liquid chromatograph system includes high performance liquid chromatograph (Waters 515)；P200 high-pressure pump；
Waters 2487 UV-detector and GJ605 type high pressure six-way injection valve；Chromatographic column be BDS HYPERSIL-C18 (4.6mm ×
250mm, 5 μm)；Data acquisition and process use HS Data Processing in Chromatography Workstation V4.0+ (Hangzhou English spectrum science and technology)
1.3 condition determinations: measure according to high performance liquid chromatography (general rule 0512).Chromatographic condition and system suitability with
Octadecylsilane chemically bonded silica is filler；With methanol-water (60:40) for flowing phase；Detection wavelength is 250nm；Theoretical plate
Number is calculated by schisandrin peak should be not less than 2000.Take schisandrin reference substance appropriate, accurately weighed, add methanol and make often
The lm l solution containing 5ug, to obtain final product.The preparation of need testing solution takes this product under content uniformity item, finely ground, takes about 0.5g, accurate
Weighed, put in 25ml measuring bottle, add methanol 20ml, supersound process (power 250W, frequency 40kHz) 30 minutes, let cool, add methanol extremely
Scale, shakes up, and filters, takes subsequent filtrate, to obtain final product.Precision draws reference substance solution and need testing solution each 20ul injection liquid phase respectively
Chromatograph, measures, to obtain final product.
1.4 experimental result
Schisandrin assay result in Fructus Schisandrae Chinensis granule prepared by each embodiment
| Sample source | Schisandrin content (mg/ bag) |
| Embodiment 1 | 3.5 |
| Embodiment 2 | 4.2 |
| Embodiment 3 | 3.8 |
| Matched group | 1.1 |
According to the result of the test of upper table, in Fructus Schisandrae Chinensis granule prepared by embodiment of the present invention 1-3, schisandrin contains
Amount is apparently higher than matched group.
Embodiment 5: the experimentation data of Fructus Schisandrae Chinensis granule of the present invention suppression Chinese hamster ovary Chinese hamster ovary celI propagation
1 experiment material
1.1 experiment cell strains
Chinese hamster ovary Chinese hamster ovary celI, Nanjing Medical University's laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: Fructus Schisandrae Chinensis granule of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: weigh 100mg Fructus Schisandrae Chinensis granule, is dissolved in 5ml dehydrated alcohol, and 0.2 μm filter filters, 500 μ l
Doff pipe subpackage ,-20 DEG C of storages, 0.2 μm filter filters dehydrated alcohol in case matched group is used simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061 Lot.No.758137)；Hyclone (Hangzhoupro, sky, Zhejiang biology section
Skill company limited Lot.No.100419)；NaHCO3 (the long hundred million chemical reagent company limited Cat.No.11810-033 in Shanghai
Lot.No.1088387)；Trypsin (AMRESCO company lot number: 2010/04)；EDTA (AMRESCO company lot number: 2009/
10)；Penicillin G Sodium Salt (AMRESCO company lot number: 2010242)；Streptomycin Sulfate
(AMRESCO company lot number: 2010382)；Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182)；MTT
(Biosharp lot number: 0793)；PBS (laboratory autogamy)；1.4 experiment equipment
Lycra inverted microscope (Germany Leica model: DM1L)；Visible-ultraviolet light microwell plate detector (MD company of the U.S.
Model: SPECTRA MAX 190)；CO2 incubator (FORMA model: 3111)；Super-clean bench (safe and sound company of Su Jing group manufacture type
Number: SW-CJ-ZFD)；Pure water instrument (Spring company of U.S. model: S/N 020579)；Accurate pipettor (Gilson Inc of France
Model: P2)；Electronic balance (Sai Duolisi company limited of Germany model: BT323S)；Full-automatic high-pressure autoclave (Japan SANYO
Company's model: MLS-3020)；Table electrothermal air dry oven (Shanghai precision experimental facilities company model: DHG9123A)；Refrigerator
(Siemens Company's model: KG18V21TI)；Liquid nitrogen container (CBS model: 2001)；Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai
Model: KA-1000)；0.2 μm filter (MILLIPORE model: SLGP033RB)；10cm culture dish (NEST company), 96 hole trainings
Support plate (NEST company)；Cell counting count board；Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) Chinese hamster ovary Chinese hamster ovary celI DMEM+10%FBS in 37 DEG C, 5%CO2 carry out cellar culture (10cm cultivate
Ware), when cell grows to logarithmic (log) phase, collect cell, discard culture fluid, PBS rinses 3 times, adds 3ml 0.25% Trypsin
Enzyme-0.04%EDTA, after 37 DEG C of digestion 2min, is added thereto in 5ml complete medium and reacts, by its turn after piping and druming cell
Entering in centrifuge tube, 1000rpm is centrifuged 5min, adjusts 3 × 104/ml of concentration of cell suspension.
2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate is put in cell culture incubator
(37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, general long to 50%-70%, add Fructus Schisandrae Chinensis particle solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts shaking table
Upper low-speed oscillation 10min, makes crystal fully dissolve.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm.
6) arranging background (being not added with cell, only add culture fluid), (cell, the medicine dissolution of same concentrations are situated between control wells simultaneously
Matter, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes.
7) suppression ratio of cell is represented by result with medicine:
Cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment weight
Multiple 3 times.
3 statistical dispositions
Use the correlation analysis in Microsoft Excel 2003 software and Studentt inspection, data with mean ±
S.D. represent.
4 experimental results
Statistical result showed after mtt assay experiment, compares with matched group, when dosage reaches 5mg/ml, to China's Hamster ova
Nest Chinese hamster ovary celI Proliferation Ability variant (P < 0.05), dosage this difference when 10mg/ml has significance (P < 0.01), works as agent
Amount reaches there is pole significant difference (P < 0.001) during 15-20mg/ml.
Fructus Schisandrae Chinensis granule is to Chinese hamster ovary Chinese hamster ovary celI Proliferation Ability influence research
Note: compare with matched group, * P < 0.01；**P<0.001
5 experiment conclusion
Fructus Schisandrae Chinensis granule can suppress Chinese hamster ovary Chinese hamster ovary celI to breed, and reduces the thin of Chinese hamster ovary Chinese hamster ovary celI
Intracellular growth number, this effect is dose dependent.
Claims (10)
1. a Fructus Schisandrae Chinensis granule, it is characterised in that preparation method is: take Fructus Schisandrae Chinensis, adds the water of 10 times of weight of schisandra chinensis medicinal material,
Soaking 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice
Decoction liquor, being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, stirs, stand, mistake
Filter, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, uses silica gel to refine, in advance will be selected
Silica gel load in resin column, after purified for described concentrated solution water being diluted, by reverse phase silica gel post, remove in liquid simultaneously
Impurity, collects whole effluent, continues to concentrate, is dried by concentrated solution, pelletizes, and fill obtains Fructus Schisandrae Chinensis granule.
Fructus Schisandrae Chinensis granule the most according to claim 1, it is characterised in that silica gel specific surface described in preparation method be 300～
500m2/ g, pore volume is 0.70～0.90ml/g, after described dilution concentrated solution by reverse phase silica gel post separate, eluting, to water outlet without
Color is only.
Fructus Schisandrae Chinensis granule the most according to claim 1, it is characterised in that concentrate for three times in said extracted method and use device, bag
Include tank body, the charging aperture being arranged on described tank body and the discharge nozzle being arranged on described tank base, described discharge nozzle sets
There are discharge valve and filter, in described tank body, are provided with shaft, described shaft is provided with stirring paddle, at described tank body
Top be provided with the stirring motor being connected with described shaft, the diapire in described tank body be provided with vibrations chamber, in described shake
Dynamic intracavity is provided with jolting plate, is provided with electromagnetic shaker, on the sidewall in described tank body between described jolting plate and described vibrations chamber
Being provided with cavity, the end face at described jolting plate is provided with scraper plate, is provided with telescopic cylinder between described scraper plate and described cavity, described
Scraper plate realizes the scraping to jolting plate surface under the effect of described telescopic cylinder, is provided with described at the center of described jolting plate
The discharging opening that discharge nozzle is connected.
Fructus Schisandrae Chinensis granule the most according to claim 3, it is characterised in that jolting plate described in enrichment facility in said extracted method
It is provided with sealing metal bar with the junction in described vibrations chamber, described tank body is provided with the vibrations control being connected with described electromagnetic shaker
Device processed.
Fructus Schisandrae Chinensis granule the most according to claim 3, it is characterised in that scraper plate described in enrichment facility in said extracted method
Bottom is provided with metal scraping layer, and described metal scraping layer is pressed on described jolting plate, is provided with and stretches with described on described tank body
The extension and contraction control button that contracting cylinders connects.
Fructus Schisandrae Chinensis granule the most according to claim 3, it is characterised in that in said extracted method in tank body described in enrichment facility
It is provided with water tester, in described discharge nozzle, is provided with cooler.
Fructus Schisandrae Chinensis granule the most according to claim 1, it is characterised in that be dried in said extracted method and use device, including case
Body, the material frame being arranged in described casing and the blast pipe and the discharge pipe that are arranged on described casing top, it is characterised in that:
It is provided with the air intake passage being connected with described blast pipe in the sidewall of described casing, the sidewall of described casing is provided with described
The air holes that air intake passage is connected, described discharge pipe through described casing roof connection casing in casing outside, described
Being additionally provided with homoiothermic chamber and sterilization chamber in the sidewall of casing, be provided with electrical heating block at described homoiothermic intracavity, described air intake passage passes
Described regulation chamber and sterilization chamber are arranged, and be provided with rotating drum, be provided with electric rotating in described rotating drum in the diapire of described casing
Machine, the main shaft of described electric rotating machine is connected with described material frame, is provided with vibrations groove in described material frame, at described material
The end face of frame is provided with and holds plate, is provided with shaking device described holding between plate and described vibrations groove.
Fructus Schisandrae Chinensis granule the most according to claim 7, it is characterised in that be dried in said extracted method to use and shake described in device
Dynamic device includes driving motor, is arranged on the eccentric on described driving electric machine main shaft, and the wheel limit of described eccentric is pressed on institute
State the bottom center holding plate, between the diapire of the described bottom holding plate and described vibrations groove, be provided with extension spring, described in draw
Spring is symmetrical arranged centered by described eccentric.
Fructus Schisandrae Chinensis granule the most according to claim 7, it is characterised in that be dried in said extracted method and use case described in device
Internal it is provided with temperature sensor, in the sidewall of described casing, is provided with the driver being connected with described temperature sensor, described
Driver is connected with the control end of described electrical heating block, is additionally provided with chamber door on described casing.
Fructus Schisandrae Chinensis granule answering in preparation suppression Chinese hamster ovary Chinese hamster ovary celI hyperproliferation agent the most according to claim 1
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610566962.2A CN106177119A (en) | 2016-07-18 | 2016-07-18 | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610566962.2A CN106177119A (en) | 2016-07-18 | 2016-07-18 | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106177119A true CN106177119A (en) | 2016-12-07 |
Family
ID=57493945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610566962.2A Withdrawn CN106177119A (en) | 2016-07-18 | 2016-07-18 | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106177119A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117278A (en) * | 2016-06-14 | 2016-11-16 | 南京华宽信息咨询中心 | The extracting method of a kind of rhodioside and rhodioside extract |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415318A (en) * | 2001-10-30 | 2003-05-07 | 国家中药制药工程技术研究中心 | Chinese herb medical preparation for treating diabetes and its preparation method |
| CN1522726A (en) * | 2003-02-21 | 2004-08-25 | 刘建生 | Novel schisandra fruit soft capsule formulation and its preparation method |
| CN101214278A (en) * | 2007-12-26 | 2008-07-09 | 沈阳药科大学 | New raw material for extracting fructus schizandrae total lignans and preparation technique and use |
| CN102302553A (en) * | 2011-07-08 | 2012-01-04 | 中国科学院过程工程研究所 | Extraction method of key components of Chinese magnoliavine fruit medicament |
| CN103550297A (en) * | 2013-11-18 | 2014-02-05 | 青岛农业大学 | Schisandra chinensis extract and preparation method thereof |
| CN103550296A (en) * | 2012-08-13 | 2014-02-05 | 范惠明 | Fructus schisandrae extract as well as preparation method and application thereof |
| CN104547035A (en) * | 2014-12-19 | 2015-04-29 | 陕西必康制药集团控股有限公司 | Extraction method of Chinese magnoliavine fruit |
-
2016
- 2016-07-18 CN CN201610566962.2A patent/CN106177119A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415318A (en) * | 2001-10-30 | 2003-05-07 | 国家中药制药工程技术研究中心 | Chinese herb medical preparation for treating diabetes and its preparation method |
| CN1522726A (en) * | 2003-02-21 | 2004-08-25 | 刘建生 | Novel schisandra fruit soft capsule formulation and its preparation method |
| CN101214278A (en) * | 2007-12-26 | 2008-07-09 | 沈阳药科大学 | New raw material for extracting fructus schizandrae total lignans and preparation technique and use |
| CN102302553A (en) * | 2011-07-08 | 2012-01-04 | 中国科学院过程工程研究所 | Extraction method of key components of Chinese magnoliavine fruit medicament |
| CN103550296A (en) * | 2012-08-13 | 2014-02-05 | 范惠明 | Fructus schisandrae extract as well as preparation method and application thereof |
| CN103550297A (en) * | 2013-11-18 | 2014-02-05 | 青岛农业大学 | Schisandra chinensis extract and preparation method thereof |
| CN104547035A (en) * | 2014-12-19 | 2015-04-29 | 陕西必康制药集团控股有限公司 | Extraction method of Chinese magnoliavine fruit |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117278A (en) * | 2016-06-14 | 2016-11-16 | 南京华宽信息咨询中心 | The extracting method of a kind of rhodioside and rhodioside extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106138928A (en) | A kind of honey refining intensified loquet cream and its preparation method and application | |
| CN106220465A (en) | The preparation method of a kind of lycopene and lycopene compositions | |
| CN105943729A (en) | Infant Qixing tea granules and preparation method thereof | |
| CN106176887A (en) | The extracting method of a kind of Fructus Momordicae and Lo Han Guo composition | |
| CN106074658A (en) | A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof | |
| CN106262638A (en) | A kind of flavouring agent | |
| CN106177119A (en) | A kind of Fructus Schisandrae Chinensis granule and preparation method thereof | |
| CN106176935A (en) | A kind of baicalin aluminium glue capsule and preparation method thereof | |
| CN106074646A (en) | The process of preparing Chinese medicine extracting method of a kind of Radix Ginseng decoction pieces and the ginseng composition of pulmonary fibrosis resistant | |
| CN106038657A (en) | Blood activating and pain stopping tablets and preparation method thereof | |
| CN204789501U (en) | A multi -functional decontaminating column that is used for chain check spore toxin to detect | |
| CN106117278A (en) | The extracting method of a kind of rhodioside and rhodioside extract | |
| CN106235331A (en) | A kind of extracting method of Arillus Longan | |
| CN106377598A (en) | Preparation method of pomelo peel total flavones and pomelo peel total flavone composition | |
| CN106074794A (en) | A kind of Houshuning Tablet and preparation method thereof | |
| CN108426960A (en) | The content assaying method of oleanolic acid in Fructus Chaenomelis and ursolic acid | |
| CN106421338A (en) | Liquid increasing granule and preparation method thereof | |
| CN105963395A (en) | Tablet for treating haemorrhoids and preparation method thereof | |
| CN111983120B (en) | Method for establishing characteristic map of wind-dispelling pill mother and measuring content of 7 nucleoside components | |
| CN106220540A (en) | The preparation method of a kind of bata-carotene and bata-carotene compositions | |
| CN106177118A (en) | A kind of five-ester soft capsule and preparation method thereof | |
| CN105943507A (en) | Cold-treating heat-clearing granules and preparation method thereof | |
| CN106235255A (en) | A kind of chafing dish material | |
| CN106177407A (en) | A kind of Qinghuozhimaipian and preparation method thereof | |
| CN106110093A (en) | A kind of wushicha granules and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20161207 |