CN106110093A - A kind of wushicha granules and its preparation method and application - Google Patents

A kind of wushicha granules and its preparation method and application Download PDF

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Publication number
CN106110093A
CN106110093A CN201610602273.2A CN201610602273A CN106110093A CN 106110093 A CN106110093 A CN 106110093A CN 201610602273 A CN201610602273 A CN 201610602273A CN 106110093 A CN106110093 A CN 106110093A
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Prior art keywords
cylinder
wushicha
granules
radix
swingle
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CN201610602273.2A
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Chinese (zh)
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卞毓平
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Nanjing Zhengkuan Pharmaceutical Technology Co Ltd
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Nanjing Zhengkuan Pharmaceutical Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J3/00—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/233—Bupleurum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/238—Saposhnikovia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/346—Platycodon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/535—Perilla (beefsteak plant)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A61K36/575—Magnolia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/634—Forsythia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8998—Hordeum (barley)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction

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Abstract

The present invention provides a kind of wushicha granules, take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, water extract-alcohol precipitation obtains concentrated solution, use supercritical extraction, obtain supercritical extract, it is prepared as wushicha granules according to a conventional method, prepare Platycodin D content high, and find wushicha granules application in preparation suppression Clonal Rat Pheochromocytoma tumor PC 12 cell proliferation.

Description

A kind of wushicha granules and its preparation method and application
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of wushicha granules and its preparation method and application.
Background technology
Prior art noon tea granule extracting method, technique is coarse, backward, and impurity is many, and active constituent content is low, causes Patient's consumption is excessive, it has not been convenient to take, and has had a strong impact on this product and has applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problems referred to above, it is an object of the invention to provide a kind of wushicha granules and preparation thereof Methods and applications.
Technical scheme: it is an object of the invention to by following scheme realization:
A kind of wushicha granules, preparation method is: take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, add the water of 10 times of weight of medical material, soak 0.5h, Decocting 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, dense Being reduced to 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, and stirring, standing, filtration, filtrate is dense Being reduced to relative density when 65 DEG C is 1.25, and reclaims ethanol, obtains concentrated solution, uses supercritical extraction, obtains supercritical extract, dry Dry, it is prepared as wushicha granules according to a conventional method.
Described wushicha granules, joins CO by water extract-alcohol precipitation concentrated solution in preparation method2In supercritical extraction device, ethanol As entrainer, it is 4-6% that entrainer accounts for the percent by volume of total extractant, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2Flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extract, and optimum condition is: described CO2It is 5% that supercritical extraction entrainer accounts for the percent by volume of total extractant, described CO2The extracting pressure of supercritical extraction 20MPa, temperature 40 DEG C, CO2Flow 2ml/g crude drug min, extraction time 160min.
Described wushicha granules, concentrates in said extracted method and uses device, including concentrating cylinder, be arranged on described concentration The vaporizer of cylinder body bottom, is provided with venthole and condenses panel, setting in described condensation panel at the top of described concentration cylinder There is cooling liquid storage chamber, be provided with the feed tube and discharge being connected with described cooling liquid storage chamber at the top of described concentration cylinder Pipe, it is characterised in that: the roof in described concentration cylinder is provided with the swingle downwardly extended, and described swingle is provided with scrapes dirt Cutterhead, the web of described scraper dish contacts with the inwall concentrating cylinder, is provided with lifting on the roof of described concentration cylinder Cylinder, the top of described swingle is connected with described lift cylinder, described through roof and the top of described concentration cylinder Being provided with electric rotating machine in swingle, the main shaft of described electric rotating machine is connected with the center of described scraper dish, in described concentration The top of cylinder is additionally provided with the elevating lever through roof, is provided with horn-like suction in the bottom of described elevating lever and steeps mouth, at described liter It is provided with in fall bar and steeps, with described horn-like suction, the suction bubble passage that mouth is connected, be provided with the absorption pump housing at the top of described elevating lever, Being provided with regulation cylinder between the roof and described elevating lever of described concentration cylinder, described elevating lever is at the work of described regulation cylinder In described concentration cylinder, lifting moving is done under with.
Described wushicha granules, the sidewall concentrating cylinder in said extracted method described in enrichment facility is provided with observation window, Being provided with seal glass in described observation window, described scraper dish is circular, the external diameter of described scraper dish and concentration cylinder Identical and scraper dish the inner ring surface of internal diameter be connected with the main shaft of electric rotating machine by dowel bar, described vaporizer for circle Tubular construction, described vaporizer is connected to concentrate the diapire of cylinder, is provided with evaporation tube in described vaporizer by adpting flange, The sidewall of described vaporizer is provided with the steam inlet being connected with described evaporation tube and steam (vapor) outlet.
Described wushicha granules, is dried in said extracted method and uses device, including hothouse, be connected with described hothouse Logical air quantity conditioning chamber, is provided with charging aperture on the roof of described hothouse, is provided with discharging opening in the bottom of described hothouse, Described air quantity conditioning chamber is provided with air intake passage, is provided with air exhaust passage on described hothouse, it is characterised in that: at described air quantity It is provided with dividing plate between conditioning chamber and described hothouse, described dividing plate is uniformly provided with pore, described dividing plate is additionally provided with side The cavity of face opening, described cavity is arranged on the side by hothouse, is provided with electric rotating machine in described cavity, is additionally provided with and institute Stating the swingle that electric rotating machine main shaft is connected, be provided with upset paddle on the surface of described swingle, described swingle stretches into In described hothouse, it is provided with air guide channel at the center of described swingle, described upset paddle is uniformly provided with described The hot gas injection jets that air guide channel is connected, when described wind is stretched in the end of described swingle in conditioning chamber, at described air guide channel Inside it is provided with heat conduction blower fan, is provided with movable plate when described wind in conditioning chamber, be provided with and described pore in the side of described movable plate The air quantity adjusting plug matched and the air holes being arranged between adjacent air quantity adjusting plug, regulate with described air quantity at described movable plate Mobile regulation cylinder it is provided with between the sidewall of room.
Described wushicha granules, is dried in said extracted method and uses the roof of described air quantity conditioning chamber described in device to be provided with Chute, the diapire at described air quantity conditioning chamber is provided with shifting chute, and described shifting chute is consistent with the trend of described chute, described movement The top of plate is movably arranged in described chute by scroll wheel, and the bottom of described movable plate is movably arranged on institute by movable block Stating in shifting chute, described mobile regulation cylinder is arranged between described movable block and the end wall of shifting chute.
Described wushicha granules, is dried in said extracted method and uses the main shaft of electric rotating machine described in device to be provided with driving Fluted disc, is provided with the teeth groove mutually ratcheting with described driving fluted disc on described swingle, and the opening part of described cavity is provided with protection network, Described swingle is arranged through described protection network.
In prior art, the original extracting method of wushicha granules, technique is coarse, backward, and impurity is many, causes patient's consumption mistake Greatly, it has not been convenient to take, Platycodin D content prepared by the present invention increases, and content is high.Concentrator structure letter in the present invention Single, simple operation, its not only can start scraper dish as required and do lift adjustment as required such that it is able to realize right The inwall concentrating cylinder carries out scraping dirt process, and the descending operation that simultaneously can also regulate elevating lever as required makes horn-like suction bubble Mouth is in being absorbed by the foam on surface just above of medication level by the absorption pump housing, solves conventional art medicine enrichment facility Middle tube wall medicine dirt removes the technical deficiency of difficulty, and stability in use is good and the suitability is strong.This drying equipment structure is simple, operation just Victory, is provided with upset paddle and is provided with, in upset paddle, the hot gas injection jets being connected with air quantity conditioning chamber, making at stirring medicine While material, medical material is dried, adds the speed contacted with medical material, can effectively improve medicinal material drying efficiency, and make With good stability, solving the technical deficiency that conventional art pharmaceutical drying device drying efficiency is low, the suitability is strong and practicality good.And Find wushicha granules application in preparation suppression Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation.
Accompanying drawing explanation
In order to make present disclosure be more likely to be clearly understood, below according to specific embodiment and combine accompanying drawing, right The present invention is described in further detail, wherein:
Fig. 1 is the structural representation of enrichment facility of the present invention.
Fig. 2 is the structural representation of drying device of the present invention.
Detailed description of the invention
Form by the following examples, is described in further detail the foregoing of the present invention again, but should be by this Being interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to Examples below, all technology realized based on foregoing of the present invention are equal Belong to the scope of the present invention.
Embodiment 1
Take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add medicine The water of 8 times of weight of material, decocts 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds second Alcohol makes the percent by volume of alcohol content reach 75%, stirs, and stands, and filters, and when filtrate is concentrated into 65 DEG C, relative density is 1.25, and Reclaim ethanol, obtain concentrated solution, use supercritical extraction, obtain supercritical extract, be dried, be prepared as tea the period of the day from 11 a.m. to 1 p.m according to a conventional method Grain.
Described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution in CO2 supercritical extraction device, ethanol conduct Entrainer, it is 4% that entrainer accounts for the percent by volume of total extractant, extracting pressure 30MPa, temperature 30 DEG C, CO2 flow 3m1/ G crude drug min, extraction time 150min, obtain supercritical extract.
See that Fig. 1: described wushicha granules, above-mentioned twice concentration use device, including concentrating cylinder 1, be arranged on described dense Vaporizer 2 bottom contracting cylinder 1, is provided with venthole 3 and condenses panel 4, at described condensation front at the top of described concentration cylinder 1 It is provided with cooling liquid storage chamber 5 in plate 4, is provided with at the top of described concentration cylinder 1 and entering that described cooling liquid storage chamber 5 is connected Liquid pipe 6 and discharge pipe 7, be provided with condensation panel, the gas partial coagulation of medicine evaporation at night can be become moisture, be possible to prevent medicine to wave Sending out, the roof in described concentration cylinder 1 is provided with the swingle 8 downwardly extended, and described swingle 8 is provided with scraper dish 9, The web of described scraper dish 9 contacts with the inwall concentrating cylinder 1, is provided with lifting air on the roof of described concentration cylinder 1 Cylinder 10, the top of described swingle 8 is connected through roof and the top of described concentration cylinder 1 with described lift cylinder 10, Being provided with electric rotating machine 11 in described swingle 8, the main shaft of described electric rotating machine 11 is connected with the center of described scraper dish 9, It is additionally provided with the elevating lever 12 through roof at the top of described concentration cylinder 1, is provided with horn-like in the bottom of described elevating lever 12 Inhale bubble mouth 13, be provided with in described elevating lever 12 and steep the suction bubble passage 14 that mouth 13 is connected, at described liter with described horn-like suction The top of fall bar 12 is provided with the absorption pump housing 15, is provided with regulation gas between the roof and described elevating lever 12 of described concentration cylinder 1 Cylinder 16, described elevating lever does lifting moving under the effect of described regulation cylinder in described concentration cylinder.
The sidewall of described concentration cylinder 1 is provided with observation window 17, in described observation window, is provided with seal glass 18.
Described scraper dish is circular, the external diameter of described scraper dish with concentrate cylinder internal diameter is identical and scraper dish Inner ring surface be connected with the main shaft of electric rotating machine by dowel bar.It is not to carry out owing to scraping dirt and inhaling bubble simultaneously, so Both can't cause mutually restriction.
Described vaporizer 2 is columnar structured, and described vaporizer 2 is connected to concentrate the end of cylinder 1 by adpting flange 19 Wall, is provided with evaporation tube 20 in described vaporizer, is provided with and is connected with described evaporation tube 20 on the sidewall of described vaporizer 2 Steam inlet 21 and steam (vapor) outlet 22.
Seeing Fig. 2: described wushicha granules, above-mentioned dry use device, including including hothouse 1 and described hothouse 1 phase The air quantity conditioning chamber 2 of connection, is provided with charging aperture 3 on the roof of described hothouse 1, is provided with out in the bottom of described hothouse 1 Material mouth 4, is provided with air intake passage 5 on described air quantity conditioning chamber 2, is provided with air exhaust passage 6, at described wind on described hothouse 1 It is provided with dividing plate 7 between amount conditioning chamber 2 and described hothouse 1, described dividing plate 7 is uniformly provided with pore 8, on described dividing plate 7 Being additionally provided with the cavity 9 of lateral opening, described cavity 9 is arranged on the side by hothouse 1, is provided with electric rotating in described cavity 9 Machine 10, is additionally provided with the swingle 11 being connected with described electric rotating machine 10 main shaft, is provided with upset on the surface of described swingle 11 Paddle 12, described swingle 11 stretches in described hothouse 1, is provided with air guide channel 13 at the center of described swingle 11, The hot gas injection jets 14 being connected with described air guide channel 13, the end of described swingle 11 it is uniformly provided with on described upset paddle 12 When portion stretches into described wind in conditioning chamber 2, in described air guide channel 13, it is provided with heat conduction blower fan 15, when described wind in conditioning chamber 2 It is provided with movable plate 16, is provided with, in the side of described movable plate 16, the air quantity adjusting plug 17 matched with described pore 8 and is arranged on Air holes 18 between adjacent air quantity adjusting plug, is provided with movement between the sidewall of described movable plate 16 and described air quantity conditioning chamber 2 Regulation cylinder 19.
Roof at described air quantity conditioning chamber 2 is provided with chute 20, and the diapire at described air quantity conditioning chamber 2 is provided with shifting chute 21, described shifting chute 21 is consistent with the trend of described chute 20, and the top of described movable plate 16 is arranged by scroll wheel 22 is movable In described chute 20, the bottom of described movable plate 16 is movably arranged in described shifting chute 21 by movable block 23, described shifting Dynamic regulation cylinder 19 is arranged between described movable block 23 and the end wall of shifting chute 21.
The main shaft of described electric rotating machine is provided with driving fluted disc 24, described swingle is provided with and described driving fluted disc Mutually ratcheting teeth groove 25.
Opening part at described cavity is provided with protection network 26, and described swingle is arranged through described protection network.
Embodiment 2
Take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add medicine The water of 8 times of weight of material, decocts 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds second Alcohol makes the percent by volume of alcohol content reach 75%, stirs, and stands, and filters, and when filtrate is concentrated into 65 DEG C, relative density is 1.25, and Reclaim ethanol, obtain concentrated solution, use supercritical extraction, obtain supercritical extract, be dried, be prepared as tea the period of the day from 11 a.m. to 1 p.m according to a conventional method Grain.
Described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution in CO2 supercritical extraction device, ethanol conduct Entrainer, it is 6% that entrainer accounts for the percent by volume of total extractant, extracting pressure 15MPa, temperature 50 C, CO2 flow 1m1/ G crude drug min, extraction time 180min, obtain supercritical extract.
Concentrate with drying equipment ibid.
Embodiment 3
Take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add medicine The water of 8 times of weight of material, decocts 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds second Alcohol makes the percent by volume of alcohol content reach 75%, stirs, and stands, and filters, and when filtrate is concentrated into 65 DEG C, relative density is 1.25, and Reclaim ethanol, obtain concentrated solution, use supercritical extraction, obtain supercritical extract, be dried, be prepared as tea the period of the day from 11 a.m. to 1 p.m according to a conventional method Grain.
Described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution in CO2 supercritical extraction device, ethanol conduct Entrainer, it is 5% that entrainer accounts for the percent by volume of total extractant, extracting pressure 20MPa of described CO2 supercritical extraction, Temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min, obtain supercritical extract.
Concentrate with drying equipment ibid.
Above-described embodiment Chinese crude drug all meets standards of pharmacopoeia.
Embodiment 4: Platycodin D assay experimentation data in wushicha granules of the present invention
1.1 Experimental agents: wushicha granules of the present invention: prepare by embodiment 1-3 method.Reference example 1 in matched group Common concentration is used to be prepared as wushicha granules with drying means.
1.2 instruments: high performance liquid chromatograph system includes high performance liquid chromatograph (Waters 515)；P200 high-pressure pump； Waters2487 UV-detector and GJ605 type high pressure six-way injection valve；Chromatographic column be Shim-Pack C18 (4.6mm × 250mm,5μm)；Data acquisition and process use HS Data Processing in Chromatography Workstation V4.0+ (Hangzhou English spectrum science and technology)
1.3 condition determinations: measure according to high performance liquid chromatography (general rule 0512).Chromatographic condition and system suitability: Chromatographic column: Shim-Pack C18 (4.6mm × 250mm, 5 μm)；Flowing phase: acetonitrile-water (volume ratio 25: 75), detects wavelength 300nm；Column temperature 40 DEG C；Sample size 10 μ l.Number of theoretical plate is calculated by Platycodin D peak should be not less than 2000.Take Platycodin D pair Appropriate according to product, accurately weighed, add methanol and make every lm l solution containing 5ug, to obtain final product.The preparation of need testing solution takes content uniformity This product under Xiang, finely ground, take about 0.5g, accurately weighed, put in 25ml measuring bottle, add methanol 20ml, supersound process (power 250W, Frequency 40kHz) 30 minutes, let cool, add methanol to scale, shake up, filter, take subsequent filtrate, to obtain final product.Precision draws reference substance respectively Solution injects chromatograph of liquid with each 20ul of need testing solution, measures, to obtain final product.
1.4 experimental result
Platycodin D assay result in wushicha granules prepared by each embodiment
Sample source Platycodin D content (%)
Embodiment 1 0.52
Embodiment 2 0.40
Embodiment 3 0.51
Matched group 0.03
According to the result of the test of upper table, Platycodin D content in wushicha granules prepared by embodiment of the present invention 1-3 Apparently higher than matched group.
Embodiment 5: the experiment of wushicha granules of the present invention suppression Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation is ground Study carefully data
1 experiment material
1.1 experiment cell strains
Clonal Rat Pheochromocytoma tumor PC-12 cell, Nanjing Medical University's laboratory cell storehouse, DMEM+10%FBS is normal Rule are cultivated.
1.2 Experimental agents
Drugs: wushicha granules of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: weigh 100mg wushicha granules, is dissolved in 5ml dehydrated alcohol, and 0.2 μm filter filters, 500 μ Ldoff pipe subpackage ,-20 DEG C of storages, 0.2 μm filter filters dehydrated alcohol in case matched group is used simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137)；Hyclone (Hangzhoupro, sky, Zhejiang biology section Skill company limited Lot.No.100419)；NaHCO3 (the long hundred million chemical reagent company limited Cat.No.11810-in Shanghai 033Lot.No.1088387)；Trypsin (AMRESCO company lot number: 2010/04)；EDTA (AMRESCO company lot number: 2009/10)；Penicillin G Sodium Salt (AMRESCO company lot number: 2010242)；Streptomycin Sulfate (AMRESCO company lot number: 2010382)；Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182)；MTT (Biosharp lot number: 0793)；PBS (laboratory autogamy)；
1.4 experiment equipment
Lycra inverted microscope (Germany Leica model: DM1L)；Visible-ultraviolet light microwell plate detector (MD company of the U.S. Model: SPECTRA MAX 190)；CO2 incubator (FORMA model: 3111)；Super-clean bench (safe and sound company of Su Jing group manufacture type Number: SW-CJ-ZFD)；Pure water instrument (Spring company of U.S. model: S/N 020579)；Accurate pipettor (Gilson Inc of France Model: P2)；Electronic balance (Sai Duolisi company limited of Germany model: BT323S)；Full-automatic high-pressure autoclave (Japan SANYO Company's model: MLS-3020)；Table electrothermal air dry oven (Shanghai precision experimental facilities company model: DHG9123A)；Refrigerator (Siemens Company's model: KG18V21TI)；Liquid nitrogen container (CBS model: 2001)；Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai Model: KA-1000)；0.2 μm filter (MILLIPORE model: SLGP033RB)；10cm culture dish (NEST company), 96 hole trainings Support plate (NEST company)；Cell counting count board；Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) Clonal Rat Pheochromocytoma tumor PC-12 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carry out conventional training Supporting (10cm culture dish), when cell grows to logarithmic (log) phase, collect cell, discard culture fluid, PBS rinses 3 times, adds 3ml 0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, is added thereto in 5ml complete medium and reacts, piping and druming Being proceeded to after cell in centrifuge tube, 1000rpm is centrifuged 5min, adjusts 3 × 104/ml of concentration of cell suspension.
2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate is put in cell culture incubator (37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, general long to 50%-70%, add wushicha granules solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts shaking table Upper low-speed oscillation 10min, makes crystal fully dissolve.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm.
6) arranging background (being not added with cell, only add culture fluid), (cell, the medicine dissolution of same concentrations are situated between control wells simultaneously Matter, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes.
7) suppression ratio of cell is represented by result with medicine:
Cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment weight Multiple 3 times.
3 statistical dispositions
Use the correlation analysis in Microsoft Excel 2003 software and Student t inspection, data with mean ± S.D. represent.
4 experimental results
Statistical result showed after mtt assay experiment, compares with matched group, when dosage reaches 5mg/ml, to adult rat adrenal tissue Pheochromocytoma PC-12 cell inhibitory effect variant (P < 0.05), dosage this difference when 10mg/ml have significance (P < 0.01) pole significant difference (P < 0.001), is had when dosage reaches 15-20mg/ml.
Wushicha granules is to Clonal Rat Pheochromocytoma tumor PC-12 cell inhibitory effect influence research (X ± SD)
Note: compare with matched group, * P < 0.01；**P<0.001
5 experiment conclusion
Wushicha granules can suppress Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation, reduce adult rat adrenal tissue addicted to The cell growing number of chromium glucagonoma PC-12 cell, this effect is dose dependent.

Claims (8)

1. a wushicha granules, it is characterised in that preparation method is: take Rhizoma Atractylodis 50g, Radix Bupleuri 50g, Rhizoma Et Radix Notopterygii 50g, Radix Saposhnikoviae 50g, Radix Angelicae Dahuricae 50g, Rhizoma Chuanxiong 50g, Herba Pogostemonis 50g, Radix Peucedani 50g, Fructus Forsythiae 50g, Pericarpium Citri Reticulatae 50g, Fructus Crataegi 50g, Fructus Aurantii Immaturus 50g, Fructus Hordei Germinatus (parched) 75g, Radix Glycyrrhizae 50g, Radix Platycodonis 75g, six Massa Medicata Fermentata preparata 50g, Folium Perillae 75g, Cortex Magnoliae Officinalis 75g, black tea 1600g, add the water of 10 times of weight of medical material, leaching Bubble 0.5h, decocts 1 hour, filters, and medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice and decocts Boiling liquid, being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, stirs, stand, mistake Filter, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, uses supercritical extraction, obtains supercritical Extract, is dried, is prepared as wushicha granules according to a conventional method.
Wushicha granules the most according to claim 1, it is characterised in that in preparation method, water extract-alcohol precipitation concentrated solution is joined CO2In supercritical extraction device, ethanol is as entrainer, and it is 4-6% that entrainer accounts for the percent by volume of total extractant, extraction pressure Power 15-30MPa, temperature 30-50 DEG C, CO2Flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical extraction Taking thing, optimum condition is: described CO2It is 5% that supercritical extraction entrainer accounts for the percent by volume of total extractant, described CO2Super Extracting pressure 20MPa of critical extraction, temperature 40 DEG C, CO2Flow 2ml/g crude drug min, extraction time 160min.
Wushicha granules the most according to claim 1, it is characterised in that concentrate in said extracted method and use device, including dense Contracting cylinder, is arranged on the vaporizer of described concentration cylinder body bottom, is provided with venthole and condensation front at the top of described concentration cylinder Plate, is provided with cooling liquid storage chamber in described condensation panel, is provided with at the top of described concentration cylinder and stores with described cooling liquid Feed tube that chamber is connected and discharge pipe, it is characterised in that: the roof in described concentration cylinder is provided with the rotation downwardly extended Bar, described swingle is provided with scraper dish, and the web of described scraper dish contacts, described dense with the inwall concentrating cylinder The roof of contracting cylinder is provided with lift cylinder, the top of described swingle through the roof of described concentration cylinder and top with described Lift cylinder is connected, and is provided with electric rotating machine, the main shaft of described electric rotating machine and described scraper dish in described swingle Center is connected, and is additionally provided with the elevating lever through roof at the top of described concentration cylinder, is provided with in the bottom of described elevating lever Horn-like suction steeps mouth, is provided with and steeps the suction bubble passage that mouth is connected, in described lifting with described horn-like suction in described elevating lever The top of bar is provided with the absorption pump housing, is provided with regulation cylinder, described liter between the roof and described elevating lever of described concentration cylinder Fall bar does lifting moving under the effect of described regulation cylinder in described concentration cylinder.
Wushicha granules the most according to claim 3, it is characterised in that concentrate cylinder in said extracted method described in enrichment facility The sidewall of body is provided with observation window, is provided with seal glass in described observation window, and described scraper dish is circular, described in scrape dirt Identical with the internal diameter concentrating cylinder and scraper dish the inner ring surface of the external diameter of cutterhead passes through the main shaft of dowel bar and electric rotating machine Being connected, described vaporizer is columnar structured, and described vaporizer is connected to concentrate the diapire of cylinder by adpting flange, in institute It is provided with evaporation tube in stating vaporizer, the sidewall of described vaporizer is provided with the steam inlet being connected with described evaporation tube and steaming Vapor outlet.
Wushicha granules the most according to claim 1, it is characterised in that be dried in said extracted method and use device, including doing The air quantity conditioning chamber that dry room is connected with described hothouse, is provided with charging aperture on the roof of described hothouse, is dried described The bottom of room is provided with discharging opening, is provided with air intake passage, is provided with air exhaust passage on described hothouse on described air quantity conditioning chamber, It is characterized in that: between described air quantity conditioning chamber and described hothouse, be provided with dividing plate, described dividing plate is uniformly provided with pore, Being additionally provided with the cavity of lateral opening on described dividing plate, described cavity is arranged on the side by hothouse, sets in described cavity There is electric rotating machine, be additionally provided with the swingle being connected with described electric rotating machine main shaft, be provided with upset on the surface of described swingle Paddle, described swingle stretches in described hothouse, is provided with air guide channel at the center of described swingle, stirs in described upset Mix and on leaf, be uniformly provided with the hot gas injection jets being connected with described air guide channel, regulation when described wind is stretched in the end of described swingle Indoor, are provided with heat conduction blower fan in described air guide channel, are provided with movable plate when described wind in conditioning chamber, at described movable plate Side is provided with described pore matches air quantity adjusting plug and the air holes being arranged between adjacent air quantity adjusting plug, in described shifting Dynamic being provided with between plate and the sidewall of described air quantity conditioning chamber moves regulation cylinder.
Wushicha granules the most according to claim 5, it is characterised in that be dried in said extracted method and use institute described in device The roof stating air quantity conditioning chamber is provided with chute, and the diapire at described air quantity conditioning chamber is provided with shifting chute, and described shifting chute is with described The trend of chute is consistent, and the top of described movable plate is movably arranged in described chute by scroll wheel, the end of described movable plate Portion is movably arranged in described shifting chute by movable block, and described mobile regulation cylinder is arranged on described movable block and shifting chute Between end wall.
Wushicha granules the most according to claim 5, it is characterised in that be dried in said extracted method to use and revolve described in device The main shaft of rotating motor is provided with driving fluted disc, is provided with the teeth groove mutually ratcheting with described driving fluted disc on described swingle, described The opening part of cavity is provided with protection network, and described swingle is arranged through described protection network.
The most according to claim 1, wushicha granules is at preparation suppression Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation Application in medicine.
CN201610602273.2A 2016-07-27 2016-07-27 A kind of wushicha granules and its preparation method and application Withdrawn CN106110093A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110638648A (en) * 2019-10-08 2020-01-03 郭娟 Chinese patent medicine preparation and processing device and method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1762474A (en) * 2005-09-30 2006-04-26 江西本草天工科技有限责任公司 Effervescence tablet for cold

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1762474A (en) * 2005-09-30 2006-04-26 江西本草天工科技有限责任公司 Effervescence tablet for cold

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张金莲 等: "《中医药学概论》", 31 December 2014, 清华大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110638648A (en) * 2019-10-08 2020-01-03 郭娟 Chinese patent medicine preparation and processing device and method

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