CN106074658A - A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof - Google Patents

A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof Download PDF

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Publication number
CN106074658A
CN106074658A CN201610543508.5A CN201610543508A CN106074658A CN 106074658 A CN106074658 A CN 106074658A CN 201610543508 A CN201610543508 A CN 201610543508A CN 106074658 A CN106074658 A CN 106074658A
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CN
China
Prior art keywords
preparation
huoxue zhirong
zhirong jiaonang
tank body
huoxue
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CN201610543508.5A
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Chinese (zh)
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李洋
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Duo Bao Bio Tech Ltd Nanjing
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Duo Bao Bio Tech Ltd Nanjing
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Priority to CN201610543508.5A priority Critical patent/CN106074658A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/32—Burseraceae (Frankincense family)
    • A61K36/324—Boswellia, e.g. frankincense
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/26—Iron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0215—Solid material in other stationary receptacles
    • B01D11/0223—Moving bed of solid material
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/06—Controlling, e.g. regulating, parameters of gas supply
    • F26B21/10—Temperature; Pressure
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
    • F26B9/06—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers
    • F26B9/08—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers including agitating devices, e.g. pneumatic recirculation arrangements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Abstract

nullThe present invention provides the preparation method of a kind of HUOXUE ZHIRONG JIAONANG，Take Radix Angelicae Sinensis 22g、Radix Notoginseng 44g、Olibanum (processed with vinegar) 44g、Borneolum Syntheticum 11g、Eupolyphaga Seu Steleophaga 111g、Pyritum (calcined) 67g，Above Six-element，Add the water of 10 times of weight of medical material，Soak 0.5h，Decoct 1 hour，Filter，Medicinal residues add the water of 8 times of weight of medical material，Decoct 1 hour，Medicinal liquid leaches，Merge twice decoction liquor，Being concentrated into 60 DEG C of relative densities is 1.05，Adding ethanol makes the percent by volume of alcohol content reach 75%，Stirring，Stand，Filter，When filtrate is concentrated into 65 DEG C, relative density is 1.25，And reclaim ethanol，Obtain concentrated solution，Use supercritical extraction，Obtain supercritical extract，Continue to concentrate，Concentrated solution is dried,Pelletize，Encapsulating capsule，Obtain HUOXUE ZHIRONG JIAONANG，And use distinctive concentration and drying equipment，In the HUOXUE ZHIRONG JIAONANG prepared, ferulaic acid content is high，Application in the concurrent suppression African green monkey kidney Vero E6 cell proliferation of preparation now.

Description

A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof.
Background technology
HUOXUE ZHIRONG JIAONANG, invigorating circulation of blood to dissipate blood stasis and reducing swelling and relieving pain.For traumatic injury, swelling and pain due to blood stasis.Preparation of the prior art Method, technique is coarse, backward, and impurity is many, and active constituent content is low, causes patient's consumption excessive, it has not been convenient to take, have a strong impact on This product is applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problems referred to above, it is an object of the invention to provide a kind of HUOXUE ZHIRONG JIAONANG and system thereof Preparation Method.
Technical scheme: it is an object of the invention to by following scheme realization:
A kind of HUOXUE ZHIRONG JIAONANG, preparation method is: take Radix Angelicae Sinensis 22g, Radix Notoginseng 44g, Olibanum (processed with vinegar) 44g, Borneolum Syntheticum 11g, ground beetle Worm 111g, Pyritum (calcined) 67g, above Six-element, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues are again Adding the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, and merges twice decoction liquor, is concentrated into 60 DEG C of relative densities and is 1.05, add ethanol and make the percent by volume of alcohol content reach 75%, stir, stand, filter, relative density when filtrate is concentrated into 65 DEG C It is 1.25, and reclaims ethanol, obtain concentrated solution, use supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is entered Row is dried, and pelletizes, and encapsulating capsule obtains HUOXUE ZHIRONG JIAONANG.
Described HUOXUE ZHIRONG JIAONANG, in above-mentioned preparation method, supercritical extraction method is: added by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 4-6% that entrainer accounts for the percent by volume of total extractant, extraction Pressure 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min, obtain supercritical Extract, optimum condition is: the percent by volume that described CO2 supercritical extraction entrainer accounts for total extractant is 5%, described Extracting pressure 20MPa of CO2 supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Described HUOXUE ZHIRONG JIAONANG, concentrates for three times in above-mentioned preparation method and uses device, including tank body, be arranged on described tank Charging aperture on body and the discharge nozzle being arranged on described tank base, be provided with discharge valve and filter on described discharge nozzle, In described tank body, it is provided with shaft, described shaft is provided with stirring paddle, be provided with at the top of described tank body and stir with described Mixing the stirring motor that axle is connected, the diapire in described tank body is provided with vibrations chamber, is provided with jolting plate at described vibrations intracavity, It is provided with electromagnetic shaker between described jolting plate and described vibrations chamber, the sidewall in described tank body is provided with cavity, in described vibrations The end face of plate is provided with scraper plate, is provided with telescopic cylinder between described scraper plate and described cavity, and described scraper plate is at described telescopic cylinder The lower scraping realized jolting plate surface of effect, be provided with the discharging being connected with described discharge nozzle at the center of described jolting plate Mouthful.
Described HUOXUE ZHIRONG JIAONANG, jolting plate described in enrichment facility and the junction in described vibrations chamber in above-mentioned preparation method It is provided with sealing metal bar, described tank body is provided with the shake control device being connected with described electromagnetic shaker.
Described HUOXUE ZHIRONG JIAONANG, in above-mentioned preparation method, the bottom of scraper plate described in enrichment facility is provided with metal scraping layer, Described metal scraping layer is pressed on described jolting plate, is provided with the flexible control being connected with described telescopic cylinder on described tank body Button processed.
Described HUOXUE ZHIRONG JIAONANG, is provided with water tester in tank body described in enrichment facility in above-mentioned preparation method, in institute It is provided with cooler in stating discharge nozzle.
Described HUOXUE ZHIRONG JIAONANG, is dried in above-mentioned preparation method and uses device, including body, be arranged in described body Axis, the rotation being arranged on described axis outer wall holds dish, is provided with vibration motor in the lower end of described axis, at described machine The top of body is provided with vapours conveyance conduit, it is characterised in that: in described axis, it is provided with cavity, sets at the top of described axis There are disk, described disk to be arranged on described rotation and hold the top of dish, described disk are provided with the most penetrating pore, in institute It is provided with the heat conduction mast being connected with described cavity in stating disk, in described cavity, is provided with electrical heating block, described heat conduction mast Being provided with heat conduction thorn post, described heat conduction thorn post stretches in described pore, is provided with temperature sensor, at described sky in described body Intracavity is provided with the driver being connected with described temperature sensor, and described driver switchs with the control of described electrical heating block and is connected Connect.
Described HUOXUE ZHIRONG JIAONANG, in above-mentioned preparation method, pore described in dry use device is the loudspeaker that big bottom, top is little Shape hole, described heat conduction thorn post stretches into the middle part in described horn-like hole, is provided with the most penetrating conduction on described horn-like hole Mesh.
Described HUOXUE ZHIRONG JIAONANG, is dried in above-mentioned preparation method and uses the bottom surface of body described in device to be provided with chamber door, institute Stating chamber door to be arranged on to rotate and hold the lowermost end of dish, described heat conduction mast be silver-colored or aluminum or copper mast.
The application in preparation suppression African green monkey kidney Vero-E6 cell proliferation of the described HUOXUE ZHIRONG JIAONANG.
In prior art, HUOXUE ZHIRONG JIAONANG uses the method that water puies forward, and technique is coarse, backward, and impurity is many, causes patient to use Measuring excessive, it has not been convenient to take, in HUOXUE ZHIRONG JIAONANG prepared by the present invention, ferulic acid yield increases, and content is high, in the present invention Concentrator simple in construction, simple operation, it is provided with jolting plate and electromagnetic shaker, and is additionally provided with scraper plate and is connected with described scraper plate Telescopic cylinder, can effectively prevent tank base from luming, be greatly improved the efficiency of heating surface to a certain extent, efficiently solve tradition Luming bottom easily occurring in technology Chinese medicinal concentration equipment and affect the technical deficiency of the efficiency of heating surface, stability in use is good and is suitable for Property strong, the stable effective ingredients of medicine is not destroyed.This drying equipment structure is simple, and simple operation is provided with at the top of axis Disk, and on disk, it is provided with pore, vapours can be made to pass through disk, and in axis, be provided with electrical heating block and heat conduction Mast, can start electrical heating block as required, and realizes temperature transfer by heat conduction mast, and with by the vapours of disk Mixing, thereby may be ensured that the temperature of vapours, regulation performance is good, and stability in use is strong, efficiently solves conventional art The uncontrollable technical deficiency of temperature in middle vapours heating drier.And find that described HUOXUE ZHIRONG JIAONANG is non-in preparation suppression Application in the grivet kidney Vero-E6 cell proliferation of continent.
Accompanying drawing explanation
In order to make present disclosure be more likely to be clearly understood, below according to specific embodiment and combine accompanying drawing, right The present invention is described in further detail, wherein:
Fig. 1 is the structural representation of enrichment facility of the present invention.
Fig. 2 is the structural representation of drying device of the present invention.
Detailed description of the invention
Form by the following examples, is described in further detail the foregoing of the present invention again, but should be by this Being interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to Examples below, all technology realized based on foregoing of the present invention are equal Belong to the scope of the present invention.
Embodiment 1
Take Radix Angelicae Sinensis 22g, Radix Notoginseng 44g, Olibanum (processed with vinegar) 44g, Borneolum Syntheticum 11g, Eupolyphaga Seu Steleophaga 111g, Pyritum (calcined) 67g, above Six-element, Adding the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 little Time, medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the volume basis of alcohol content Than reaching 75%, stirring, standing, filtration, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, makes With supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain promoting blood circulation and stopping pain Capsule.
The preparation method of described HUOXUE ZHIRONG JIAONANG, described supercritical extraction method is: added by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 4% that entrainer accounts for the percent by volume of total extractant, extraction pressure Power 30MPa, temperature 30 DEG C, CO2 flow 3m1/g crude drug min, extraction time 150min, obtain supercritical extract.
See Fig. 1: the preparation method of described HUOXUE ZHIRONG JIAONANG, above-mentioned No. three enrichment facilities, including tank body 1, be arranged on institute State the charging aperture 2 on tank body 1 and be arranged on the discharge nozzle 3 bottom described tank body 1, described discharge nozzle 3 is provided with discharge valve 4 With filter 5, in described tank body 1, it is provided with shaft 6, described shaft 6 is provided with stirring paddle 7, on the top of described tank body 1 Portion is provided with the stirring motor 8 being connected with described shaft 6, and the diapire in described tank body 1 is provided with vibrations chamber 9, in described shake It is provided with jolting plate 10 in dynamic chamber 9, between described jolting plate 10 and described vibrations chamber 9, is provided with electromagnetic shaker 11, can by electromagnetic shaker So that jolting plate vibrations, thus can effectively prevent drug particles from concentrating on jolting plate, thus drug particles can be prevented in vibrations Luming on plate, the sidewall in described tank body 1 is provided with cavity 12, the end face at described jolting plate 10 is provided with scraper plate 13, in institute State and between scraper plate 13 and described cavity 12, be provided with telescopic cylinder 14, telescopic cylinder can be controlled as required, make scraper plate in vibrations The surface of plate scrapes, thus the drug particles that part concentrates at jolting plate surface scrapes, and described scraper plate 13 is at described flexible gas The effect of cylinder 14 is lower realizes the scraping to jolting plate 10 surface, is provided with at the center of described jolting plate 10 and described discharge nozzle 3 phase The discharging opening 15 of connection.
It is provided with sealing metal bar 16 in the junction of described jolting plate 10 with described vibrations chamber 9, described tank body is provided with The shake control device 17 being connected with described electromagnetic shaker.
Be provided with metal scraping layer 18 in the bottom of described scraper plate, described metal scraping layer is pressed on described jolting plate, Described tank body is provided with the extension and contraction control button 19 being connected with described telescopic cylinder.
In described tank body, it is provided with water tester 21, in described discharge nozzle, is provided with cooler 20.
See Fig. 2, the preparation method of described HUOXUE ZHIRONG JIAONANG, above-mentioned dry use device, including body 1, be arranged on institute Stating the axis 2 in body 1, the rotation being arranged on described axis 2 outer wall holds dish 3, is provided with vibrations in the lower end of described axis 2 Motor 4, is provided with vapours conveyance conduit 5 at the top of described body 1, is provided with cavity 6 in described axis 2, at described axis 2 Top be provided with disk 7, described disk 7 is arranged on described rotation and holds the top of dish 3, is provided with and leads to up and down on described disk 7 Saturating pore 8, vapours through disk, enters rotation and holds dish, is provided with and is connected with described cavity 6 in described disk 7 Heat conduction mast 9, is provided with electrical heating block 10 in described cavity 6, and described heat conduction mast 9 is provided with heat conduction thorn post 11, described heat conduction Thorn post 11 stretches in described pore 8, can be greatly improved the temperature mixing between vapours and electrical heating block, improves temperature and controls Efficiency, in described body 1, be provided with temperature sensor 12, be provided with in described cavity 6 and be connected with described temperature sensor 12 The driver 13 connect, described driver 13 switchs with the control of described electrical heating block 10 and is connected.Temperature sensing in the present embodiment Device and driver are the most already present product, and it has only to be attached i.e. according to the corresponding description of product in use Can.
Described pore is the horn-like hole that big bottom, top is little, and described heat conduction thorn post stretches into the middle part in described horn-like hole, Described horn-like hole is provided with the most penetrating conduction mesh 14.
Be provided with chamber door 15 in the bottom surface of described body, described chamber door is arranged on and rotates the lowermost end holding dish.
Described heat conduction mast is silver or aluminum or copper mast.
Embodiment 2
Take Radix Angelicae Sinensis 22g, Radix Notoginseng 44g, Olibanum (processed with vinegar) 44g, Borneolum Syntheticum 11g, Eupolyphaga Seu Steleophaga 111g, Pyritum (calcined) 67g, above Six-element, Adding the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 little Time, medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the volume basis of alcohol content Than reaching 75%, stirring, standing, filtration, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, makes With supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain promoting blood circulation and stopping pain Capsule.
The preparation method of described HUOXUE ZHIRONG JIAONANG, described supercritical extraction method is: added by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 6% that entrainer accounts for the percent by volume of total extractant, extraction pressure Power 15MPa, temperature 50 C, CO2 flow 1m1/g crude drug min, extraction time 180min, obtain supercritical extract.
Concentrate with drying device ibid.
Embodiment 3
Take Radix Angelicae Sinensis 22g, Radix Notoginseng 44g, Olibanum (processed with vinegar) 44g, Borneolum Syntheticum 11g, Eupolyphaga Seu Steleophaga 111g, Pyritum (calcined) 67g, above Six-element, Adding the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 little Time, medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the volume basis of alcohol content Than reaching 75%, stirring, standing, filtration, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, makes With supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain promoting blood circulation and stopping pain Capsule.
The preparation method of described HUOXUE ZHIRONG JIAONANG, described supercritical extraction method is: added by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 5% that entrainer accounts for the percent by volume of total extractant, described Extracting pressure 20MPa of CO2 supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min, Supercritical extract.
Concentrate with drying device ibid.
Above-described embodiment Chinese medicine all meets standards of pharmacopoeia.
Embodiment 4: ferulaic acid content determination experiment research data in HUOXUE ZHIRONG JIAONANG extract of the present invention
1.1 Experimental agents: HUOXUE ZHIRONG JIAONANG of the present invention: prepare by embodiment 1-3 method.Matched group uses pharmacopeia mark Standard, common method for concentration prepares HUOXUE ZHIRONG JIAONANG.
1.2 instruments: high performance liquid chromatograph system includes high performance liquid chromatograph (Waters 515)；P200 high-pressure pump； Waters2487 UV-detector and GJ605 type high pressure six-way injection valve；Chromatographic column be Hypersil ODSC18 post (4.6mm × 250mm,5μm)；Data acquisition and process use HS Data Processing in Chromatography Workstation V4.0+ (Hangzhou English spectrum science and technology)
1.3 condition determinations: employing high performance liquid chromatography, employing chromatographic column Hypersil ODSC18 post (4.6mm × 250mm,5μm)；Flowing phase: methanol: water=75:25；Flow velocity is 1.2mL/min；Column temperature is 30 DEG C；Detection wavelength be 268～ 270nm。
1.4 experimental result
Ferulaic acid content measurement result in HUOXUE ZHIRONG JIAONANG prepared by each embodiment
Sample source Ferulaic acid content (%)
Embodiment 1 1.68
Embodiment 2 1.94
Embodiment 3 1.65
Matched group 0.69
According to the result of the test of upper table, in HUOXUE ZHIRONG JIAONANG prepared by embodiment of the present invention 1-3, peaceful ferulic acid contains Amount is apparently higher than matched group.
Embodiment 5: the experimentation data of HUOXUE ZHIRONG JIAONANG of the present invention suppression African green monkey kidney Vero-E6 cell proliferation
1 experiment material
1.1 experiment cell strains
African green monkey kidney Vero-E6 cell, Nanjing Medical University's laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: HUOXUE ZHIRONG JIAONANG of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: weigh 100mg HUOXUE ZHIRONG JIAONANG, is dissolved in 5ml dehydrated alcohol, and 0.2 μm filter filters, 500 μ l Doff pipe subpackage ,-20 DEG C of storages, 0.2 μm filter filters dehydrated alcohol in case matched group is used simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137)；Hyclone (Hangzhoupro, sky, Zhejiang biology section Skill company limited Lot.No.100419)；NaHCO3 (the long hundred million chemical reagent company limited Cat.No.11810-in Shanghai 033Lot.No.1088387)；Trypsin (AMRESCO company lot number: 2010/04)；EDTA (AMRESCO company lot number: 2009/10)；Penicillin G Sodium Salt (AMRESCO company lot number: 2010242)；Streptomycin Sulfate (AMRESCO company lot number: 2010382)；Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182)；MTT (Biosharp lot number: 0793)；PBS (laboratory autogamy)；1.4 experiment equipment
Lycra inverted microscope (Germany Leica model: DM1L)；Visible-ultraviolet light microwell plate detector (MD company of the U.S. Model: SPECTRA MAX 190)；CO2 incubator (FORMA model: 3111)；Super-clean bench (safe and sound company of Su Jing group manufacture type Number: SW-CJ-ZFD)；Pure water instrument (Spring company of U.S. model: S/N 020579)；Accurate pipettor (Gilson Inc of France Model: P2)；Electronic balance (Sai Duolisi company limited of Germany model: BT323S)；Full-automatic high-pressure autoclave (Japan SANYO Company's model: MLS-3020)；Table electrothermal air dry oven (Shanghai precision experimental facilities company model: DHG9123A)；Refrigerator (Siemens Company's model: KG18V21TI)；Liquid nitrogen container (CBS model: 2001)；Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai Model: KA-1000)；0.2 μm filter (MILLIPORE model: SLGP033RB)；10cm culture dish (NEST company), 96 hole trainings Support plate (NEST company)；Cell counting count board；Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) African green monkey kidney Vero-E6 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carry out cellar culture (10cm train Support ware), when cell grows to logarithmic (log) phase, collect cell, discard culture fluid, PBS rinses 3 times, adds 3ml0.25% Trypsin Enzyme-0.04%EDTA, after 37 DEG C of digestion 2min, is added thereto in 5ml complete medium and reacts, by its turn after piping and druming cell Entering in centrifuge tube, 1000rpm is centrifuged 5min, adjusts 3 × 104/ml of concentration of cell suspension.
2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate is put in cell culture incubator (37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, general long to 50%-70%, add HUOXUE ZHIRONG JIAONANG solution, continue to cultivate 24h。
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts shaking table Upper low-speed oscillation 10min, makes crystal fully dissolve.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm.
6) arranging background (being not added with cell, only add culture fluid), (cell, the medicine dissolution of same concentrations are situated between control wells simultaneously Matter, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes.
7) suppression ratio of cell is represented by result with medicine:
Cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment weight Multiple 3 times.
3 statistical dispositions
Use the correlation analysis in Microsoft Excel 2003 software and Student t inspection, data with mean ± S.D. represent.
4 experimental results
Statistical result showed after mtt assay experiment, compares with matched group, when dosage reaches 5mg/ml, to African green monkey kidney Vero-E6 cell inhibitory effect variant (P < 0.05), dosage this difference when 10mg/ml has significance (P < 0.01), when Dosage reaches there is pole significant difference (P < 0.001) during 15-20mg/ml.
HUOXUE ZHIRONG JIAONANG is to African green monkey kidney Vero-E6 cell inhibitory effect influence research
Note: compare with matched group, * P < 0.01；**P<0.001
5 experiment conclusion
HUOXUE ZHIRONG JIAONANG can suppress African green monkey kidney Vero-E6 cell proliferation, reduces African green monkey kidney Vero-E6 thin The cell growing number of born of the same parents, this effect is dose dependent.

Claims (10)

1. a HUOXUE ZHIRONG JIAONANG, it is characterised in that preparation method is: take Radix Angelicae Sinensis 22g, Radix Notoginseng 44g, Olibanum (processed with vinegar) 44g, Borneolum Syntheticum 11g, Eupolyphaga Seu Steleophaga 111g, Pyritum (calcined) 67g, above Six-element, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, mistake Filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, are concentrated into 60 DEG C relatively Density is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, and stirring, standing, filtration, filtrate is concentrated into 65 DEG C of phases It is 1.25 to density, and reclaims ethanol, obtain concentrated solution, use supercritical extraction, obtain supercritical extract, continue to concentrate, by dense Contracting liquid is dried, and pelletizes, and encapsulating capsule obtains HUOXUE ZHIRONG JIAONANG.
HUOXUE ZHIRONG JIAONANG the most according to claim 1, it is characterised in that in described preparation method, supercritical extraction method is: Water extract-alcohol precipitation concentrated solution is joined CO2In supercritical extraction device, ethanol is as entrainer, and entrainer accounts for the body of total extractant Long-pending percentage ratio is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2Flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, and optimum condition is: described CO2Supercritical extraction entrainer accounts for the body of total extractant Long-pending percentage ratio is 5%, described CO2Extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO2Flow 2ml/g crude drug min, Extraction time 160min.
HUOXUE ZHIRONG JIAONANG the most according to claim 1, it is characterised in that concentrate for three times in described preparation method and use device, Including tank body, the charging aperture being arranged on described tank body and the discharge nozzle being arranged on described tank base, on described discharge nozzle It is provided with discharge valve and filter, in described tank body, is provided with shaft, described shaft is provided with stirring paddle, at described tank The top of body is provided with the stirring motor being connected with described shaft, and the diapire in described tank body is provided with vibrations chamber, described Vibrations intracavity is provided with jolting plate, is provided with electromagnetic shaker, the sidewall in described tank body between described jolting plate and described vibrations chamber Being provided with cavity, the end face at described jolting plate is provided with scraper plate, is provided with telescopic cylinder, institute between described scraper plate and described cavity State scraper plate under the effect of described telescopic cylinder, realize the scraping to jolting plate surface, be provided with and institute at the center of described jolting plate State the discharging opening that discharge nozzle is connected.
HUOXUE ZHIRONG JIAONANG the most according to claim 3, it is characterised in that shake described in enrichment facility in described preparation method Plate is provided with sealing metal bar with the junction in described vibrations chamber, is provided with the vibrations being connected with described electromagnetic shaker on described tank body Controller.
HUOXUE ZHIRONG JIAONANG the most according to claim 3, it is characterised in that scraper plate described in enrichment facility in described preparation method Bottom be provided with metal scraping layer, described metal scraping layer is pressed on described jolting plate, is provided with described on described tank body The extension and contraction control button that telescopic cylinder is connected.
HUOXUE ZHIRONG JIAONANG the most according to claim 3, it is characterised in that tank body described in enrichment facility in described preparation method Inside it is provided with water tester, in described discharge nozzle, is provided with cooler.
HUOXUE ZHIRONG JIAONANG the most according to claim 1, it is characterised in that be dried in described preparation method and use device, including Body, is arranged on the axis in described body, and the rotation being arranged on described axis outer wall holds dish, in the lower end of described axis It is provided with vibration motor, is provided with vapours conveyance conduit at the top of described body, it is characterised in that: in described axis, it is provided with sky Chamber, is provided with disk at the top of described axis, and described disk is arranged on described rotation and holds the top of dish, sets on described disk There is the most penetrating pore, in described disk, be provided with the heat conduction mast being connected with described cavity, be provided with in described cavity Electrical heating block, described heat conduction mast is provided with heat conduction thorn post, and described heat conduction thorn post stretches in described pore, sets in described body Having temperature sensor, be provided with the driver being connected with described temperature sensor in described cavity, described driver is with described The control switch of electrical heating block is connected.
HUOXUE ZHIRONG JIAONANG the most according to claim 7, it is characterised in that be dried in described preparation method and use described in device Pore is the horn-like hole that big bottom, top is little, and described heat conduction thorn post stretches into the middle part in described horn-like hole, described horn-like Hole is provided with the most penetrating conduction mesh.
HUOXUE ZHIRONG JIAONANG the most according to claim 7, it is characterised in that be dried in described preparation method and use described in device The bottom surface of body is provided with chamber door, and described chamber door is arranged on to rotate and holds the lowermost end of dish, and described heat conduction mast be silver-colored or aluminum or copper Matter mast.
The most according to claim 1, HUOXUE ZHIRONG JIAONANG is in preparation suppression African green monkey kidney Vero-E6 cell proliferation Application.
CN201610543508.5A 2016-07-11 2016-07-11 A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof Withdrawn CN106074658A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109106743A (en) * 2018-09-28 2019-01-01 珠海安生凤凰制药有限公司 A kind of blood-activating and pain-stopping capsules and preparation method thereof
CN109269277A (en) * 2018-09-15 2019-01-25 江西植提庄园健康产业有限公司 A kind of borneol blast drier
CN111595108A (en) * 2020-06-01 2020-08-28 云南昊辰食品有限公司 Vacuum freeze-drying method for preserving effective components in angelica sinensis medicinal material

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1438009A (en) * 2003-03-10 2003-08-27 南京中山制药厂 Method for making capsule for promoting blood-circulation and relieving pain
CN1939378A (en) * 2006-09-25 2007-04-04 南京中山制药有限公司 Preparation of blood-activating and pain-stopping capsules
CN102319281A (en) * 2011-09-30 2012-01-18 珠海安生凤凰制药有限公司 Traditional Chinese medicine preparation for invigorating blood circulation and relieving pain and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1438009A (en) * 2003-03-10 2003-08-27 南京中山制药厂 Method for making capsule for promoting blood-circulation and relieving pain
CN1939378A (en) * 2006-09-25 2007-04-04 南京中山制药有限公司 Preparation of blood-activating and pain-stopping capsules
CN102319281A (en) * 2011-09-30 2012-01-18 珠海安生凤凰制药有限公司 Traditional Chinese medicine preparation for invigorating blood circulation and relieving pain and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
国家药典委员会编: "《中华人民共和国药典 2010年版 第二增补本》", 30 September 2013, 中国医药科技出版社 *
罗永明: "《中药化学成分提取分离技术与方法》", 31 January 2016, 上海科学技术出版社 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109269277A (en) * 2018-09-15 2019-01-25 江西植提庄园健康产业有限公司 A kind of borneol blast drier
CN109106743A (en) * 2018-09-28 2019-01-01 珠海安生凤凰制药有限公司 A kind of blood-activating and pain-stopping capsules and preparation method thereof
CN109106743B (en) * 2018-09-28 2021-07-20 珠海安生凤凰制药有限公司 Blood circulation promoting and pain relieving capsule and preparation method thereof
CN111595108A (en) * 2020-06-01 2020-08-28 云南昊辰食品有限公司 Vacuum freeze-drying method for preserving effective components in angelica sinensis medicinal material

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