CN106075125A - A kind of YIXUANNING JIAONANG and preparation method thereof - Google Patents

A kind of YIXUANNING JIAONANG and preparation method thereof Download PDF

Info

Publication number
CN106075125A
CN106075125A CN201610542432.4A CN201610542432A CN106075125A CN 106075125 A CN106075125 A CN 106075125A CN 201610542432 A CN201610542432 A CN 201610542432A CN 106075125 A CN106075125 A CN 106075125A
Authority
CN
China
Prior art keywords
yixuanning
jiaonang
preparation
tank body
heat conduction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201610542432.4A
Other languages
Chinese (zh)
Inventor
李洋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duo Bao Bio Tech Ltd Nanjing
Original Assignee
Duo Bao Bio Tech Ltd Nanjing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duo Bao Bio Tech Ltd Nanjing filed Critical Duo Bao Bio Tech Ltd Nanjing
Priority to CN201610542432.4A priority Critical patent/CN106075125A/en
Publication of CN106075125A publication Critical patent/CN106075125A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/26—Iron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/287—Chrysanthemum, e.g. daisy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/539—Scutellaria (skullcap)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A61K36/8884—Arisaema, e.g. Jack in the pulpit
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Inorganic Chemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Neurosurgery (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention provides the preparation method of a kind of YIXUANNING JIAONANG, take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Radix Scutellariae 15g, Caulis Bambusae In Taenia 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, Pericarpium Citri Reticulatae 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, above 12 tastes, add the water of 10 times of weight of medical material, and use distinctive concentration and drying equipment, in the YIXUANNING JIAONANG prepared, Determination of Total Glucosides of Paeony is high, the application in the concurrent suppression African green monkey kidney Vero E6 cell proliferation of preparation now.

Description

A kind of YIXUANNING JIAONANG and preparation method thereof
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of YIXUANNING JIAONANG and preparation method thereof.
Background technology
YIXUANNING JIAONANG energy suppressing the hyperactive liver and subsiding YANG, pathogenic fire reducing clearing away phlegm, spleen invigorating of nourishing blood, expelling wind and clearing away heat, cure mainly excessive rising of liver-YANG, QI and blood deficiency Type vertigo.Preparation method of the prior art, technique is coarse, backward, and impurity is many, and active constituent content is low, causes patient to use Measure excessive, it has not been convenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problems referred to above, it is an object of the invention to provide a kind of YIXUANNING JIAONANG and preparation thereof Method.
Technical scheme: it is an object of the invention to by following scheme realization:
A kind of YIXUANNING JIAONANG, preparation method is: take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Radix Scutellariae 15g, bamboo Eat 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, Pericarpium Citri Reticulatae 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, and above 12 Taste, adds the water of 10 times of weight of medical material, soaks 0.5h, decocts 1 hour, filters, and medicinal residues add the water of 8 times of weight of medical material, decocts 1 Hour, medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the volume hundred of alcohol content Proportion by subtraction reaches 75%, stirs, and stands, and filters, and when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, Use supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain YIXUANNING Capsule.
Described YIXUANNING JIAONANG, in above-mentioned preparation method, supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 4-6% that entrainer accounts for the percent by volume of total extractant, extraction pressure Power 15-30MPa, temperature 30-50 DEG C, CO2 flow 1-3ml/g crude drug min, extraction time 150-180min, obtain supercritical extraction Taking thing, optimum condition is: the percent by volume that described CO2 supercritical extraction entrainer accounts for total extractant is 5%, described CO2 Extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min.
Described YIXUANNING JIAONANG, concentrates for three times in above-mentioned preparation method and uses device, including tank body, be arranged on described tank body On charging aperture and be arranged on the discharge nozzle of described tank base, described discharge nozzle is provided with discharge valve and filter, It is provided with shaft in described tank body, described shaft is provided with stirring paddle, be provided with and described stirring at the top of described tank body The stirring motor that axle is connected, the diapire in described tank body is provided with vibrations chamber, is provided with jolting plate at described vibrations intracavity, in institute State and be provided with electromagnetic shaker between jolting plate and described vibrations chamber, the sidewall in described tank body is provided with cavity, at described jolting plate End face be provided with scraper plate, between described scraper plate and described cavity, be provided with telescopic cylinder, described scraper plate is at described telescopic cylinder Effect is lower realizes the scraping to jolting plate surface, is provided with the discharging being connected with described discharge nozzle at the center of described jolting plate Mouthful.
Described YIXUANNING JIAONANG, in above-mentioned preparation method, jolting plate described in enrichment facility sets with the junction in described vibrations chamber There is sealing metal bar, described tank body is provided with the shake control device being connected with described electromagnetic shaker.
Described YIXUANNING JIAONANG, in above-mentioned preparation method, the bottom of scraper plate described in enrichment facility is provided with metal scraping layer, institute State metal scraping layer to be pressed on described jolting plate, described tank body is provided with the extension and contraction control being connected with described telescopic cylinder Button.
Described YIXUANNING JIAONANG, is provided with water tester in tank body described in enrichment facility in above-mentioned preparation method, described Cooler it is provided with in discharge nozzle.
Described YIXUANNING JIAONANG, is dried in above-mentioned preparation method and uses device, including body, be arranged in described body Axis, the rotation being arranged on described axis outer wall holds dish, is provided with vibration motor in the lower end of described axis, at described body Top be provided with vapours conveyance conduit, it is characterised in that: in described axis, be provided with cavity, be provided with at the top of described axis Disk, described disk is arranged on described rotation and holds the top of dish, is provided with the most penetrating pore, described on described disk It is provided with the heat conduction mast being connected with described cavity in disk, in described cavity, is provided with electrical heating block, on described heat conduction mast Being provided with heat conduction thorn post, described heat conduction thorn post stretches in described pore, is provided with temperature sensor, at described cavity in described body Inside being provided with the driver being connected with described temperature sensor, described driver switchs with the control of described electrical heating block and is connected Connect.
Described YIXUANNING JIAONANG, in above-mentioned preparation method, pore described in dry use device is the loudspeaker that big bottom, top is little Shape hole, described heat conduction thorn post stretches into the middle part in described horn-like hole, is provided with the most penetrating conduction net on described horn-like hole Hole.
Described YIXUANNING JIAONANG, is dried in above-mentioned preparation method and uses the bottom surface of body described in device to be provided with chamber door, described Chamber door is arranged on to rotate and holds the lowermost end of dish, and described heat conduction mast be silver-colored or aluminum or copper mast.
The application in preparation suppression African green monkey kidney Vero-E6 cell proliferation of the described YIXUANNING JIAONANG.
In prior art, YIXUANNING JIAONANG uses the method that water puies forward, and technique is coarse, backward, and impurity is many, causes patient's consumption Excessive, it has not been convenient to take, in YIXUANNING JIAONANG prepared by the present invention, Radix Paeoniae Alba total glucosides yield increases, and content is high, dense in the present invention Contracting device structure is simple, simple operation, it is provided with jolting plate and electromagnetic shaker, and is additionally provided with scraper plate and is connected with described scraper plate Telescopic cylinder, can effectively prevent tank base from luming, be greatly improved the efficiency of heating surface to a certain extent, efficiently solves tradition skill Caking bottom easily occurring in art Chinese medicinal concentration equipment and affect the technical deficiency of the efficiency of heating surface, stability in use is good and the suitability By force, the stable effective ingredients of medicine is not destroyed.This drying equipment structure is simple, and simple operation is provided with circle at the top of axis Dish, and on disk, it is provided with pore, vapours can be made to pass through disk, and in axis, be provided with electrical heating block and heating column Bar, can start electrical heating block as required, and realize temperature transfer by heat conduction mast, and enter with by the vapours of disk Row mixing, thereby may be ensured that the temperature of vapours, and regulation performance is good, and stability in use is strong, efficiently solves in conventional art The uncontrollable technical deficiency of temperature in vapours heating drier.And find that described YIXUANNING JIAONANG is green in preparation suppression Africa Application in monkey kidney Vero-E6 cell proliferation.
Accompanying drawing explanation
In order to make present disclosure be more likely to be clearly understood, below according to specific embodiment and combine accompanying drawing, right The present invention is described in further detail, wherein:
Fig. 1 is the structural representation of enrichment facility of the present invention.
Fig. 2 is the structural representation of drying device of the present invention.
Detailed description of the invention
Form by the following examples, is described in further detail the foregoing of the present invention again, but should be by this Being interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to Examples below, all technology realized based on foregoing of the present invention are equal Belong to the scope of the present invention.
Embodiment 1
Take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Radix Scutellariae 15g, Caulis Bambusae In Taenia 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, old Skin 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, and above 12 tastes add the water of 10 times of weight of medical material, soak 0.5h, decocts 1 hour, filters, and medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction Liquid, being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, stirs, stand, filter, When filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, uses supercritical extraction, obtains supercritical extraction Take thing, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain YIXUANNING JIAONANG.
The preparation method of described YIXUANNING JIAONANG, described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 4% that entrainer accounts for the percent by volume of total extractant, extracting pressure 30MPa, temperature 30 DEG C, CO2 flow 3m1/g crude drug min, extraction time 150min, obtain supercritical extract.
See Fig. 1: the preparation method of described YIXUANNING JIAONANG, above-mentioned No. three enrichment facilities, including tank body 1, be arranged on described Charging aperture on tank body 12 and be arranged on the discharge nozzle 3 bottom described tank body 1, is provided with discharge valve 4 He on described discharge nozzle 3 Filter 5, is provided with shaft 6 in described tank body 1, is provided with stirring paddle 7 on described shaft 6, at the top of described tank body 1 Being provided with the stirring motor 8 being connected with described shaft 6, the diapire in described tank body 1 is provided with vibrations chamber 9, in described vibrations It is provided with jolting plate 10 in chamber 9, between described jolting plate 10 and described vibrations chamber 9, is provided with electromagnetic shaker 11, permissible by electromagnetic shaker Make jolting plate shake, thus can effectively prevent drug particles from concentrating on jolting plate, thus drug particles can be prevented at jolting plate Upper caking, the sidewall in described tank body 1 is provided with cavity 12, and the end face at described jolting plate 10 is provided with scraper plate 13, described It is provided with telescopic cylinder 14 between scraper plate 13 and described cavity 12, telescopic cylinder can be controlled as required, make scraper plate at jolting plate Surface scrape, thus part is concentrated at the drug particles on jolting plate surface and scrapes, described scraper plate 13 is at described telescopic cylinder The effect of 14 is lower realizes the scraping to jolting plate 10 surface, is provided with at the center of described jolting plate 10 and is connected with described discharge nozzle 3 Logical discharging opening 15.
It is provided with sealing metal bar 16 in the junction of described jolting plate 10 with described vibrations chamber 9, described tank body is provided with The shake control device 17 being connected with described electromagnetic shaker.
Be provided with metal scraping layer 18 in the bottom of described scraper plate, described metal scraping layer is pressed on described jolting plate, Described tank body is provided with the extension and contraction control button 19 being connected with described telescopic cylinder.
In described tank body, it is provided with water tester 21, in described discharge nozzle, is provided with cooler 20.
See Fig. 2, the preparation method of described YIXUANNING JIAONANG, above-mentioned dry use device, including body 1, be arranged on described Axis 2 in body 1, the rotation being arranged on described axis 2 outer wall holds dish 3, is provided with vibrations electricity in the lower end of described axis 2 Machine 4, is provided with vapours conveyance conduit 5 at the top of described body 1, is provided with cavity 6 in described axis 2, at described axis 2 Top is provided with disk 7, and described disk 7 is arranged on described rotation and holds the top of dish 3, is provided with the most penetrating on described disk 7 Pore 8, vapours through disk, enters rotation and holds dish, be provided with and leading that described cavity 6 is connected in described disk 7 Plume bar 9, is provided with electrical heating block 10 in described cavity 6, and described heat conduction mast 9 is provided with heat conduction thorn post 11, and described heat conduction is stung Post 11 stretches in described pore 8, can be greatly improved the temperature mixing between vapours and electrical heating block, improves temperature controlled Efficiency, is provided with temperature sensor 12 in described body 1, is provided with is connected with described temperature sensor 12 in described cavity 6 Driver 13, the control of described driver 13 and described electrical heating block 10 switchs and is connected.Temperature sensor in the present embodiment Being the most already present product with driver, it has only to be attached according to the corresponding description of product in use.
Described pore is the horn-like hole that big bottom, top is little, and described heat conduction thorn post stretches into the middle part in described horn-like hole, Described horn-like hole is provided with the most penetrating conduction mesh 14.
Be provided with chamber door 15 in the bottom surface of described body, described chamber door is arranged on and rotates the lowermost end holding dish.
Described heat conduction mast is silver or aluminum or copper mast.
Embodiment 2
Take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Radix Scutellariae 15g, Caulis Bambusae In Taenia 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, old Skin 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, and above 12 tastes add the water of 10 times of weight of medical material, soak 0.5h, decocts 1 hour, filters, and medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction Liquid, being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, stirs, stand, filter, When filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, uses supercritical extraction, obtains supercritical extraction Take thing, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain YIXUANNING JIAONANG.
The preparation method of described YIXUANNING JIAONANG, described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 6% that entrainer accounts for the percent by volume of total extractant, extracting pressure 15MPa, temperature 50 C, CO2 flow 1m1/g crude drug min, extraction time 180min, obtain supercritical extract.
Concentrate with drying device ibid.
Embodiment 3
Take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Radix Scutellariae 15g, Caulis Bambusae In Taenia 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, old Skin 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, and above 12 tastes add the water of 10 times of weight of medical material, soak 0.5h, decocts 1 hour, filters, and medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, and medicinal liquid leaches, and merges twice decoction Liquid, being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes the percent by volume of alcohol content reach 75%, stirs, stand, filter, When filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, obtains concentrated solution, uses supercritical extraction, obtains supercritical extraction Take thing, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, obtain YIXUANNING JIAONANG.
The preparation method of described YIXUANNING JIAONANG, described supercritical extraction method is: joined by water extract-alcohol precipitation concentrated solution In CO2 supercritical extraction device, ethanol is as entrainer, and it is 5% that entrainer accounts for the percent by volume of total extractant, described CO2 Extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO2 flow 2ml/g crude drug min, extraction time 160min, obtain super Critical extract.
Concentrate with drying device ibid.
Above-described embodiment Chinese medicine all meets standards of pharmacopoeia.
Embodiment 4: Determination of Total Glucosides of Paeony determination experiment research data in YIXUANNING JIAONANG of the present invention
1.1 Experimental agents: YIXUANNING JIAONANG of the present invention: prepare by embodiment 1-3 method.Matched group uses ministry standard, Common method for concentration prepares YIXUANNING JIAONANG.
1.2 instruments: high performance liquid chromatograph system includes high performance liquid chromatograph (Waters 515)；P200 high-pressure pump； Waters 2487 UV-detector and GJ605 type high pressure six-way injection valve；Data acquisition and process use the work of HS chromatographic data Stand V4.0+ (Hangzhou English spectrum science and technology)
1.3 condition determinations: use high performance liquid chromatography, with octadecylsilane chemically bonded silica for fixing phase, acetonitrile- 0.1% phosphoric acid (25:75) is flowing phase, detects wavelength 230nm, flow velocity 1.0mL/min, measures benzoic acid content, thus calculate Go out Determination of Total Glucosides of Paeony.
1.4 experimental result
Determination of Total Glucosides of Paeony measurement result in YIXUANNING JIAONANG prepared by each embodiment
Sample source Determination of Total Glucosides of Paeony (%)
Embodiment 1 1.46
Embodiment 2 1.68
Embodiment 3 1.73
Matched group 0.53
According to the result of the test of upper table, in YIXUANNING JIAONANG prepared by embodiment of the present invention 1-3, peaceful Radix Paeoniae Alba total glucosides contains Amount is apparently higher than matched group.
Embodiment 5: the experimentation data of YIXUANNING JIAONANG of the present invention suppression African green monkey kidney Vero-E6 cell proliferation
1 experiment material
1.1 experiment cell strains
African green monkey kidney Vero-E6 cell, Jilin Province just with Pharmaceutical Group Plc laboratory cell storehouse, DMEM + 10%FBS cellar culture.
1.2 Experimental agents
Drugs: YIXUANNING JIAONANG of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: weigh 100mg YIXUANNING JIAONANG, is dissolved in 5ml dehydrated alcohol, and 0.2 μm filter filters, 500 μ l Doff pipe subpackage ,-20 DEG C of storages, 0.2 μm filter filters dehydrated alcohol in case matched group is used simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137)；Hyclone (Hangzhoupro, sky, Zhejiang biology section Skill company limited Lot.No.100419)；NaHCO3 (the long hundred million chemical reagent company limited Cat.No.11810-in Shanghai 033Lot.No.1088387)；Trypsin (AMRESCO company lot number: 2010/04)；EDTA (AMRESCO company lot number: 2009/10)；Penicillin G Sodium Salt (AMRESCO company lot number: 2010242)；Streptomycin Sulfate (AMRESCO company lot number: 2010382)；Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182)；MTT (Biosharp lot number: 0793)；PBS (laboratory autogamy)；1.4 experiment equipment
Lycra inverted microscope (Germany Leica model: DM1L)；Visible-ultraviolet light microwell plate detector (MD company of the U.S. Model: SPECTRA MAX 190)；CO2 incubator (FORMA model: 3111)；Super-clean bench (safe and sound company of Su Jing group manufacture type Number: SW-CJ-ZFD)；Pure water instrument (Spring company of U.S. model: S/N 020579)；Accurate pipettor (Gilson Inc of France Model: P2)；Electronic balance (Sai Duolisi company limited of Germany model: BT323S)；Full-automatic high-pressure autoclave (Japan SANYO Company's model: MLS-3020)；Table electrothermal air dry oven (Shanghai precision experimental facilities company model: DHG9123A)；Refrigerator (Siemens Company's model: KG18V21TI)；Liquid nitrogen container (CBS model: 2001)；Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai Model: KA-1000)；0.2 μm filter (MILLIPORE model: SLGP033RB)；10cm culture dish (NEST company), 96 hole trainings Support plate (NEST company)；Cell counting count board；Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) African green monkey kidney Vero-E6 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carry out cellar culture (10cm train Support ware), when cell grows to logarithmic (log) phase, collect cell, discard culture fluid, PBS rinses 3 times, adds 3ml0.25% Trypsin Enzyme-0.04%EDTA, after 37 DEG C of digestion 2min, is added thereto in 5ml complete medium and reacts, by its turn after piping and druming cell Entering in centrifuge tube, 1000rpm is centrifuged 5min, adjusts 3 × 104/ml of concentration of cell suspension.
2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate is put in cell culture incubator (37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, general long to 50%-70%, add YIXUANNING JIAONANG solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts shaking table Upper low-speed oscillation 10min, makes crystal fully dissolve.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm.
6) arranging background (being not added with cell, only add culture fluid), (cell, the medicine dissolution of same concentrations are situated between control wells simultaneously Matter, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes.
7) suppression ratio of cell is represented by result with medicine:
Cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment weight Multiple 3 times.
3 statistical dispositions
Use the correlation analysis in Microsoft Excel 2003 software and Student t inspection, data with mean ± S.D. represent.
4 experimental results
Statistical result showed after mtt assay experiment, compares with matched group, when dosage reaches 5mg/ml, to African green monkey kidney Vero-E6 cell inhibitory effect variant (P < 0.05), dosage this difference when 10mg/ml has significance (P < 0.01), when Dosage reaches there is pole significant difference (P < 0.001) during 15-20mg/ml.
YIXUANNING JIAONANG is to African green monkey kidney Vero-E6 cell inhibitory effect influence research
Note: compare with matched group, * P < 0.01；**P<0.001
5 experiment conclusion
YIXUANNING JIAONANG can suppress African green monkey kidney Vero-E6 cell proliferation, reduces African green monkey kidney Vero-E6 cell Cell growing number, this effect is dose dependent.

Claims (10)

1. a YIXUANNING JIAONANG, it is characterised in that preparation method is: take Fructus Xanthii (parched) 20g, Flos Chrysanthemi 15g, Arisaema Cum Bile 15g, Huang A kind of reed mentioned in ancient books 15g, Caulis Bambusae In Taenia 15g, Concha Ostreae (calcined) 20g, Fructus Crataegi 10g, Pericarpium Citri Reticulatae 15g, Radix Paeoniae Alba 15g, the pig iron fall 15g, Poria 20g, Fructus Lycii 20g, Above 12 tastes, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, and medicinal residues add 8 times of weight of medical material Water, decocts 1 hour, and medicinal liquid leaches, and merges twice decoction liquor, and being concentrated into 60 DEG C of relative densities is 1.05, adds ethanol and makes alcohol content Percent by volume reach 75%, stirring, stand, filter, when filtrate is concentrated into 65 DEG C, relative density is 1.25, and reclaims ethanol, Obtain concentrated solution, use supercritical extraction, obtain supercritical extract, continue to concentrate, concentrated solution is dried, pelletize, encapsulating capsule, Obtain YIXUANNING JIAONANG.
YIXUANNING JIAONANG the most according to claim 1, it is characterised in that in described preparation method, supercritical extraction method is: will Water extract-alcohol precipitation concentrated solution joins CO2In supercritical extraction device, ethanol is as entrainer, and entrainer accounts for the volume of total extractant Percentage ratio is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO2Flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, and optimum condition is: described CO2Supercritical extraction entrainer accounts for the body of total extractant Long-pending percentage ratio is 5%, described CO2Extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO2Flow 2ml/g crude drug min, Extraction time 160min.
YIXUANNING JIAONANG the most according to claim 1, it is characterised in that concentrate for three times in described preparation method and use device, bag Include tank body, the charging aperture being arranged on described tank body and the discharge nozzle being arranged on described tank base, described discharge nozzle sets There are discharge valve and filter, in described tank body, are provided with shaft, described shaft is provided with stirring paddle, at described tank body Top be provided with the stirring motor being connected with described shaft, the diapire in described tank body be provided with vibrations chamber, in described shake Dynamic intracavity is provided with jolting plate, is provided with electromagnetic shaker, on the sidewall in described tank body between described jolting plate and described vibrations chamber Being provided with cavity, the end face at described jolting plate is provided with scraper plate, is provided with telescopic cylinder between described scraper plate and described cavity, described Scraper plate realizes the scraping to jolting plate surface under the effect of described telescopic cylinder, is provided with described at the center of described jolting plate The discharging opening that discharge nozzle is connected.
YIXUANNING JIAONANG the most according to claim 3, it is characterised in that jolting plate described in enrichment facility in described preparation method It is provided with sealing metal bar with the junction in described vibrations chamber, described tank body is provided with the vibrations control being connected with described electromagnetic shaker Device processed.
YIXUANNING JIAONANG the most according to claim 3, it is characterised in that scraper plate described in enrichment facility in described preparation method Bottom is provided with metal scraping layer, and described metal scraping layer is pressed on described jolting plate, is provided with and stretches with described on described tank body The extension and contraction control button that contracting cylinders connects.
YIXUANNING JIAONANG the most according to claim 3, it is characterised in that in described preparation method in tank body described in enrichment facility It is provided with water tester, in described discharge nozzle, is provided with cooler.
YIXUANNING JIAONANG the most according to claim 1, it is characterised in that be dried in described preparation method and use device, including machine Body, is arranged on the axis in described body, and the rotation being arranged on described axis outer wall holds dish, sets in the lower end of described axis There is vibration motor, be provided with vapours conveyance conduit at the top of described body, it is characterised in that: in described axis, it is provided with sky Chamber, is provided with disk at the top of described axis, and described disk is arranged on described rotation and holds the top of dish, sets on described disk There is the most penetrating pore, in described disk, be provided with the heat conduction mast being connected with described cavity, be provided with in described cavity Electrical heating block, described heat conduction mast is provided with heat conduction thorn post, and described heat conduction thorn post stretches in described pore, sets in described body Having temperature sensor, be provided with the driver being connected with described temperature sensor in described cavity, described driver is with described The control switch of electrical heating block is connected.
YIXUANNING JIAONANG the most according to claim 7, it is characterised in that be dried described in use device thin in described preparation method Hole is the horn-like hole that big bottom, top is little, and described heat conduction thorn post stretches into the middle part in described horn-like hole, in described horn-like hole It is provided with the most penetrating conduction mesh.
YIXUANNING JIAONANG the most according to claim 7, it is characterised in that be dried in described preparation method and use machine described in device The bottom surface of body is provided with chamber door, and described chamber door is arranged on and rotates the lowermost end holding dish, and described heat conduction mast is silver or aluminum or copper Mast.
The most according to claim 1, YIXUANNING JIAONANG is in preparation suppression African green monkey kidney Vero-E6 cell proliferation Application.
CN201610542432.4A 2016-07-11 2016-07-11 A kind of YIXUANNING JIAONANG and preparation method thereof Withdrawn CN106075125A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610542432.4A CN106075125A (en) 2016-07-11 2016-07-11 A kind of YIXUANNING JIAONANG and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610542432.4A CN106075125A (en) 2016-07-11 2016-07-11 A kind of YIXUANNING JIAONANG and preparation method thereof

Publications (1)

Publication Number Publication Date
CN106075125A true CN106075125A (en) 2016-11-09

Family

ID=57219777

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610542432.4A Withdrawn CN106075125A (en) 2016-07-11 2016-07-11 A kind of YIXUANNING JIAONANG and preparation method thereof

Country Status (1)

Country Link
CN (1) CN106075125A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102359941A (en) * 2011-10-07 2012-02-22 通化正和药业有限公司 Yixuanning capsule, its identification and content determining method
CN103341074A (en) * 2013-07-29 2013-10-09 通化茂祥制药有限公司 Application of Yixuanning granules in preparation of medicaments for treating insomnia
CN104840797A (en) * 2015-05-28 2015-08-19 吉林省正和药业集团股份有限公司 Preparation method and application of Yixuanning capsule

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102359941A (en) * 2011-10-07 2012-02-22 通化正和药业有限公司 Yixuanning capsule, its identification and content determining method
CN103341074A (en) * 2013-07-29 2013-10-09 通化茂祥制药有限公司 Application of Yixuanning granules in preparation of medicaments for treating insomnia
CN104840797A (en) * 2015-05-28 2015-08-19 吉林省正和药业集团股份有限公司 Preparation method and application of Yixuanning capsule

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
罗永明: "《中药化学成分提取分离技术与方法》", 31 January 2016, 上海科学技术出版社 *

Similar Documents

Publication Publication Date Title
CN102973667A (en) Preparation method and application of cold-fever tablet
CN106138928A (en) A kind of honey refining intensified loquet cream and its preparation method and application
CN105853474A (en) Ginkgolide extracting method and ginkgolide extract
CN106074658A (en) A kind of HUOXUE ZHIRONG JIAONANG and preparation method thereof
CN102988480B (en) Method for preparing bile and patchouli rhinitis tablets and application
CN102988489B (en) Preparation method and application of nose comforting tablet
CN103599292B (en) A kind of preparation method of Guishaoliujun Pill and application
CN103495117A (en) Preparation method and application of thrombus removing and collaterals dredging tablets
CN102973749B (en) Preparation method and applications of divaricate saposhnikovia-angelica archang lica rhinitis tablets
CN105963395A (en) Tablet for treating haemorrhoids and preparation method thereof
CN106075125A (en) A kind of YIXUANNING JIAONANG and preparation method thereof
CN106176935A (en) A kind of baicalin aluminium glue capsule and preparation method thereof
CN104940426A (en) Preparation method and application of osteoproliferation removal tablets
CN106177407A (en) A kind of Qinghuozhimaipian and preparation method thereof
CN103479749B (en) Preparation method and application of Gegenqinlian tablet
CN106177118A (en) A kind of five-ester soft capsule and preparation method thereof
CN102988611B (en) Preparation method and application of antihypertension tablet
CN105727020A (en) Preparation method and application of granules for treating cold and clearing heat
CN106038657A (en) Blood activating and pain stopping tablets and preparation method thereof
CN105998841A (en) Heart quieting and nerve soothing capsules and preparation method thereof
CN106110093A (en) A kind of wushicha granules and its preparation method and application
CN106177119A (en) A kind of Fructus Schisandrae Chinensis granule and preparation method thereof
CN103536691B (en) A kind of preparation method of Schisandrae Pill and application
CN103536725B (en) A kind of preparation method of priceless health restoring powder and application
CN102988522B (en) Preparation method and application of naodesheng tablets

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20161109