CN101507751A - Use of Prunella vulgaris extract in preparing medicine for treating insomnia - Google Patents
Use of Prunella vulgaris extract in preparing medicine for treating insomnia Download PDFInfo
- Publication number
- CN101507751A CN101507751A CNA2009100302104A CN200910030210A CN101507751A CN 101507751 A CN101507751 A CN 101507751A CN A2009100302104 A CNA2009100302104 A CN A2009100302104A CN 200910030210 A CN200910030210 A CN 200910030210A CN 101507751 A CN101507751 A CN 101507751A
- Authority
- CN
- China
- Prior art keywords
- extract
- spica prunellae
- medicine
- prunella vulgaris
- insomnia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of Chinese medicine, and relates to new application of prunella vulgaris L. extracts in preparing a medicine for treating insomnia. Prunella vulgaris L. as a raw material is extracted by ethanol, and ethanol extract is degreased by sherwood oil to prepare prunella vulgaris L. extract. Results of pharmacodynamic tests of sedation and hypnogenesis prove that the prunella vulgaris L. extract has obvious activity of sedation and hypnogenesis, and can be used for preparing a medicine for treating insomnia and health-care food for regulating sleep. When insomnia is treated, the prunella vulgaris L. extract as an active ingredient can be independently used or prepared into a medicine of any preparation clinically matched with other medicines.
Description
Technical field
The invention belongs to technical field of Chinese medicines, relate to the new purposes of a kind of Spica Prunellae extract aspect preparation Cure for insomnia medicine.
Background technology
Spica Prunellae (Spica Prunellea) is the dry fruit ear of Labiatae Prunella plant Spica Prunellae (Prunellea Valgaris L.).Traditional medicine thinks that Spica Prunellae nature and flavor toil is cold, goes into the liver and gall warp, has the effect of heat-clearing and toxic substances removing, hard masses softening and resolving.Modern pharmacology discovers that Spica Prunellae has blood pressure lowering, blood sugar lowering, antibiotic, antiviral, cell toxicant, antiinflammatory and various active such as immunomodulating and antitumor.Clinically, Spica Prunellae is widely used at aspects such as treatment hypertension, diabetes, cyclomastopathy, tuberculosis, hepatitis, tumor and insomnias.Separation and analytical chemistry are discovered number of chemical compositions such as containing triterpenes, sterols, flavonoid, the plain class of phenylpropyl alcohol, phenolic acids, saccharide and volatile oil in the Spica Prunellae,, be the main active of Spica Prunellae wherein based on the triterpenes components of ursolic acid, oleanolic acid and more based on the liposoluble ingredient content of rosmarinic acid, caffeic acid.Ursolic acid has multiple biological activitys such as anti-inflammatory and antalgic, antibiotic, antiviral, antitumor, anti-liver injury, and that rosmarinic acid has is antibiotic, antiviral, antiinflammatory and various active such as immunomodulating, antioxidation.The Spica Prunellae total saponins is relevant with hypotensive effect, has tangible anti-AIDS toxic action in addition; The Spica Prunellae polysaccharide has significant immunostimulation; Tannin in the Spica Prunellae may be the main active of the 6-helical bundle configuration of its anti-HIV-1 gp41; Spica Prunellae water extract 200mg/ml has remarkable inhibitory action to HIV-1 protease, also has immunoregulatory activity; The Spica Prunellae ethanol extract has the effect that antiadrenergic drug hyperamization sugar raises, and also has antiinflammatory and immunosuppressant isoreactivity.Chinese patent CN101040905A discloses a kind of hypoglycemic drug with the Spica Prunellae preparation, and Spica Prunellae total triterpenic acids content wherein is more than or equal to 50%.Patent CN1331488C discloses a kind of Spica Prunellae extract application in preparation treatment rheumatic arthritis medicine and preparation method thereof.Patent CN101317883A discloses a kind of Prunella spike active site application in preparation treatment colon cancer medicine and preparation method thereof, and this extract is the water-saturated n-butanol extract.Patent CN1012474012A discloses compositions, preparation method and the medicinal usage thereof of Prunella plant extract, and said composition can be used for preparation treatment cyclomastopathy, thyroid, pulmonary tuberculosis, coronary heart disease, angina pectoris, hypertension, hyperglycemia, cervical erosion and spectrum antibacterials.Patent CN101347502A provides two kinds of active components of Spica Prunellae and preparation method thereof and the application in preparation medicine for treating tumor thing.
In Ancient Times in China relevant record is just arranged with the Spica Prunellae Cure for insomnia, " Chongqing hall random notes " record Spica Prunellae " have the effect with positive yin nourishing among the eliminating stagnation concurrently, the person's of being insomnia clothes promptly sleeps after losing blood "." herbal classic is dredged card " record Spica Prunellae energy " logical negative and positive ... control sleeplessness ".The modern insomnia with the Spica Prunellae compound treatment clinically also obtained good curative effect.But only see the direct Spica Prunellae Cure for insomnia of using so far, do not see the calmness about Spica Prunellae extract, the research report of syngignoscism, the application of Spica Prunellae ethanol extract in preparation Cure for insomnia medicine especially do not appear in the newspapers.
Summary of the invention
The object of the present invention is to provide a kind of Spica Prunellae ethanol extract at preparation Cure for insomnia medicine or as the new purposes of regulating in the sleep health food.
Spica Prunellae ethanol extract of the present invention makes by the following method: with the Spica Prunellae medical material is raw material, uses ethanol extraction, filters, and alcohol extract concentrates and reclaims ethanol, and gained extractum defat with petroleum ether filters, and gets solid, is drying to obtain.
The calmness of above-mentioned Spica Prunellae ethanol extract, hypnotic activity test result show that this extract has tangible calmness, syngignoscism, can be used for the treatment of insomnia.
When being applied to prepare the Cure for insomnia medicine, be active component with the Spica Prunellae ethanol extract, with the preparation process of routine, use separately or prepare the medicine of operable arbitrary dosage form clinically with other drug.
The specific embodiment
Embodiment 1, get Spica Prunellae medical material 100g, with 95% alcohol reflux of 8 times of amounts of medical material 3 times, each 2 hours, filter, alcohol extract concentrates and reclaims ethanol, and gained extractum filters with petroleum ether (30 ℃～60 ℃) defat, solid in 50 ℃ of baking ovens dry 2 hours obtains Spica Prunellae ethanol extract 3.27g.
Embodiment 2, get Spica Prunellae medical material 100g, with 80% alcohol reflux of 10 times of amounts of medical material 3 times, each 2 hours, filter, alcohol extract concentrates and reclaims ethanol, petroleum ether (30 ℃～60 ℃) defat of gained extractum, solid in 50 ℃ of baking ovens dry 2 hours obtains Spica Prunellae ethanol extract 3.93g.
Embodiment 3, get Spica Prunellae medical material 100g, with 60% alcohol reflux of 12 times of amounts of medical material 3 times, each 2 hours, filter, alcohol extract concentrates and reclaims ethanol, petroleum ether (30 ℃～60 ℃) defat of gained extractum, solid in 60 ℃ of baking ovens dry 2 hours obtains Spica Prunellae ethanol extract 4.76g.
With the behavioral pharmacology experimental technique calmness, the hypnotic activity of Spica Prunellae ethanol extract are tested.
Drug effect embodiment 1, calm pharmacodynamic experiment
Investigate the sedative activity that is subjected to the reagent thing by influence to the mice autonomic activities.
1, experiment material
1.1 medicine and reagent
The Spica Prunellae ethanol extract: lot number 071207, the normal saline solution that is mixed with crude drug concentration and is 1g/mL is standby.
Diazepam (Zhejiang Medicine Co, lot number 051030): the normal saline solution that is mixed with 0.2mg/mL concentration.
Normal saline: Hunan Cologne Pharmaceutical Co., Ltd, the accurate word H51021158 of traditional Chinese medicines.
1.2 animal
Healthy KM mice, body weight 20 ± 2g, male and female half and half are provided by Jiangsu University's Experimental Animal Center.
1.3 instrument
The multi-functional mice autonomic activities of YLS-1A monitor, Shandong Academy of Medical Sciences.
1.4 statistical method
Data are handled with the SAS6.12 statistical software, and the result represents with x ± s, compares the significance of difference between each administration group and the matched group with t-check between group.* represent p＜0.05, represent that this administration group and matched group comparing difference are remarkable; * represents p＜0.01, represents this administration group and matched group comparing difference highly significant, and * * * represents p＜0.001, and it is very remarkable to show this administration group and matched group comparing difference.
2, experimental technique
Test mice fasting in preceding 8 hours water inlet, be divided into blank group, positive controls during experiment at random and be subjected to reagent thing group, 10 every group, male and female half and half; Each organizes the administration volume in 0.4mL/20g, gastric infusion.Positive controls administration diazepam (dosage is by 4mg/kg).Behind the administration 30min mice is put into the active box of autonomic activities monitor, the movable number of times of mice in the record 5min gets free movable number of times (being the mean activity number of times of every mice) behind the adaptation 3min.
Autonomic activities suppression ratio (%)=(blank group of activity number of times-medicine group activity number of times)/blank group of activity number of times * 100%
3, experimental result
Table 1 is the situation that influences to the mice autonomic activities, and as seen, the Spica Prunellae ethanol extract is 36.56% to the suppression ratio of mice autonomic activities, and is remarkable with blank group comparing difference to the inhibitory action of mice autonomic activities, suitable with the positive controls effect.
Table 1 Spica Prunellae ethanol extract is to the influence (n=10) of mice autonomic activities
Compare with the blank group,
*P＜0.05
4, conclusion
The Spica Prunellae ethanol extract has tangible sedation.
Pharmacodynamic experiment example 2, hypnosis pharmacodynamic experiment
Investigate the hypnotic activity that is subjected to the reagent thing by synergism to the pentobarbital sodium mice sleep.
1, experiment material
1.1 medicine and reagent
The Spica Prunellae ethanol extract: lot number 071207, the normal saline solution that is mixed with crude drug concentration and is 1g/mL is standby.
Pentobarbital sodium: serva company, Shanghai chemical reagent packing factory, batch number 84-06-12, the normal saline solution that is mixed with 0.75mg/mL and two concentration of 2.0mg/mL is standby.
Normal saline: Hunan Cologne Pharmaceutical Co., Ltd, the accurate word H51021158 of traditional Chinese medicines.
1.2 animal
Healthy KM mice, body weight 20 ± 2g, male and female half and half are provided by Jiangsu University's Experimental Animal Center.
1.3 statistical method
Data are handled with the SAS6.12 statistical software, and the result represents with x ± s, compares the significance of difference between each administration group and the matched group with t-check between group.* represent p＜0.05, represent that this administration group and matched group comparing difference are remarkable; * represents p＜0.01, represents this administration group and matched group comparing difference highly significant, and * * * represents p＜0.001, represents that this administration group and matched group comparing difference are very remarkable.
2, experimental technique
2.1 prolong the experiment length of one's sleep
Test mice fasting in preceding 8 hours water inlet, be divided into blank group during experiment at random and be subjected to reagent thing group, 10 every group, male and female half and half; Each organizes the administration volume in 0.4mL/20g, gastric infusion.Behind each administration group administration 30min and the blank group lumbar injection pentobarbital sodium of dosage (35mg/kg) above threshold together.With mice after the administration anyway areflexia to reach 1min be sleeping index, anyway areflexia to the time of recovering to be experienced is the length of one's sleep, the length of one's sleep of mice respectively organized in record, the length of one's sleep (being the average length of one's sleep of every mice).
2.2 increase sleeping number of elements experiment
Test mice fasting in preceding 8 hours water inlet, be divided into the blank group during experiment at random and be subjected to reagent thing group, 10 every group, male and female half and half; Each organizes the administration volume in 0.4mL/20g, gastric infusion.Behind each administration group administration 30min and blank organize the pentobarbital sodium of lumbar injection sub-threshold dose (20mg/kg) together, with mice in the 15min after the administration anyway areflexia to reach 1min be sleeping index, observed and recorded is respectively organized the mice number of elements of falling asleep.
3, experimental result
Table 2 is the hypnosis experimental result of Spica Prunellae ethanol extract, as seen ethanol extract has the prolongation effect to dosage pentobarbital sodium mouse sleep time above threshold, and compare significant difference with the blank group, and can obviously increase the sleeping number of elements of sub-threshold dose pentobarbital sodium mice, show that the Spica Prunellae ethanol extract is remarkable to the synergism of pentobarbital sodium.
The syngignoscism (n=10) of table 2 Spica Prunellae ethanol extract
Compare with the blank group,
*P＜0.05
4, conclusion
The Spica Prunellae ethanol extract has tangible syngignoscism.
Claims (2)
1, the purposes of Spica Prunellae extract in preparation Cure for insomnia medicine is characterized in that the health food that can be used to prepare Cure for insomnia class medicine or regulate sleep as preparation.
2, the described Spica Prunellae extract of claim 1 makes by the following method: Spica Prunellae medical material ethanol extraction, and filtration merges alcohol extract, concentrates to reclaim ethanol, and gained extractum defat with petroleum ether filters, and gets solid, is drying to obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100302104A CN101507751A (en) | 2009-03-17 | 2009-03-17 | Use of Prunella vulgaris extract in preparing medicine for treating insomnia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100302104A CN101507751A (en) | 2009-03-17 | 2009-03-17 | Use of Prunella vulgaris extract in preparing medicine for treating insomnia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101507751A true CN101507751A (en) | 2009-08-19 |
Family
ID=41000388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009100302104A Pending CN101507751A (en) | 2009-03-17 | 2009-03-17 | Use of Prunella vulgaris extract in preparing medicine for treating insomnia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101507751A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106303A (en) * | 2015-09-21 | 2015-12-02 | 上海中医药大学 | Medical application of selfheal aqueous extract |
| CN107913305A (en) * | 2016-10-10 | 2018-04-17 | 北京中医药大学 | Prunella vulgaris extract and its preparation method and application |
| CN107913304A (en) * | 2016-10-10 | 2018-04-17 | 北京中医药大学 | Dripping pill containing Prunella vulgaris extract and preparation method thereof |
-
2009
- 2009-03-17 CN CNA2009100302104A patent/CN101507751A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106303A (en) * | 2015-09-21 | 2015-12-02 | 上海中医药大学 | Medical application of selfheal aqueous extract |
| CN107913305A (en) * | 2016-10-10 | 2018-04-17 | 北京中医药大学 | Prunella vulgaris extract and its preparation method and application |
| CN107913304A (en) * | 2016-10-10 | 2018-04-17 | 北京中医药大学 | Dripping pill containing Prunella vulgaris extract and preparation method thereof |
| CN107913304B (en) * | 2016-10-10 | 2020-10-02 | 北京中医药大学 | Dripping pill containing Prunellae Spica extract and its preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103784933B (en) | Recurrent oral ulceration falls apart | |
| CN113288978A (en) | Composition for treating gout and preparation method and application thereof | |
| CN104523930B (en) | A kind of Pericarpium Citri Reticulatae Rhizoma Atractylodis Macrocephalae compositions reducing emesis of chemotherapy | |
| CN102579554A (en) | Peanut stem and leaf extract and preparation method as well as application thereof | |
| CN101507751A (en) | Use of Prunella vulgaris extract in preparing medicine for treating insomnia | |
| CN101130055B (en) | Method for producing medicament for treating headache | |
| CN101933973B (en) | Medicament composition for preventing and treating liver damage | |
| CN109464505B (en) | Traditional Chinese medicine composition for treating diabetes | |
| CN101579405B (en) | Traditional Chinese medicine for curing constipation and preparation method thereof | |
| CN101120977B (en) | Medicine for treating tumor | |
| CN104804056A (en) | Sarcopyramis nepalensis extract and application thereof | |
| CN102188483B (en) | Extract for treating pharyngolaryngitis and preparation method thereof | |
| CN103566349B (en) | A kind of pharmaceutical composition for the treatment of hyperglycemia hyperlipidemia | |
| CN101480472B (en) | Medicament for reducing blood pressure and regulating blood fat and production method | |
| CN1899586A (en) | Chinese medicine preparation for treating gynecologic inflammation and its preparing method | |
| CN1709415A (en) | Asparagus extract and its preparing method | |
| CN102204956B (en) | Chinese medicinal composition used at stroke recovery period and preparation method thereof | |
| CN101507752A (en) | Prunella vulgaris extract dispersible tablets and preparation method and use thereof | |
| CN101780120A (en) | Medicinal effect activities of extract of mixture of Poria cum Ligno Hospite and ganoderma and application in medicine | |
| CN104547026A (en) | Preparation method and application of salvia miltiorrhiza leave and panax pseudo-ginseng extract | |
| CN103494849A (en) | New use of asiatic pennywort herb extract in treatment of hyperlipidaemia and related medicines | |
| CN101444609B (en) | Traditional Chinese medicine with drug treatment function and preparation technique thereof | |
| CN103127412B (en) | Pharmaceutical composition, and preparation method and application thereof | |
| CN104547027B (en) | Preparation method and application of salvia miltiorrhiza leave and panax notoginseng leaf extract | |
| CN103211864B (en) | Melastoma dodecandrum Lour extract product and application of Melastoma dodecandrum Lour in preparation of medicines for treating AIDS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090819 |