CN1226909C

CN1226909C - Production method of tobacco germination accelerating type coated pelleted seeds

Info

Publication number: CN1226909C
Application number: CN 03135483
Authority: CN
Inventors: 白永富; 卢秀萍; 张恒; 郭生云; 邓云龙; 李永平; 方敦煌; 肖炳光; 马文广
Original assignee: Inst Of Agriculture Yunnan I O
Current assignee: Inst Of Agriculture Yunnan I O
Priority date: 2003-07-23
Filing date: 2003-07-23
Publication date: 2005-11-16
Anticipated expiration: 2023-07-23
Also published as: CN1481663A

Abstract

The invention discloses a production method of a tobacco germination accelerating type pelleted seed. Soaking selected tobacco seeds in 0.5 percent copper sulfate aqueous solution, taking out the tobacco seeds, washing the tobacco seeds by clear water, and airing the tobacco seeds; putting the mixture into a distilled water bottle filled with tap water, and then putting the mixture into a climatic chamber for accelerating germination, wherein the accelerating germination temperature is 25-28 ℃, and the illumination time of the climatic chamber is light/dark alternation for 12 hours during accelerating germination; accelerating germination for 30-40 hours; when most of seeds have broken seed coats, taking the seeds out of the distilled water bottle and drying the seeds; and then nucleating, pelleting, shaping, polishing, dyeing and drying the seeds by the conventional technology to obtain the required tobacco germination accelerating type pelleted seeds. The invention firstly carries out germination acceleration under certain conditions on the tobacco seeds and then coats the tobacco seeds to produce the tobacco germination acceleration type pelleted seeds with high activity.

Description

One production method that grows tobacco vernalization type coat pelleted seeds

Technical field

The present invention relates to the production method of tobacco seed, more particularly, the present invention relates to a production method that grows tobacco vernalization type coat pelleted seeds.

Background technology

As everyone knows, flue-cured tobacco is a kind of important economic crops, and the cigarette seedling is the basis that the flue-cured tobacco land for growing field crops produces.Cultivating of the right age, neat, healthy and strong anosis cigarette seedling is the important step that realizes flue-cured tobacco high-quality, suitable product, high benefit.Floating seedlings is an advanced person's a seedling raising manners, has the technical characterictic of intensive management, and its production efficiency height, flourishing, the neat and consistent of cigarette shoot root system are suitable for the specialization and the scale of leaf tobacco production, simultaneously also for cultivating anosis and giving security strong sprout.Country such as the U.S., Brazil is just wideling popularize this new technology, and popularity rate reaches more than 80%.

China was from the floating seedlings that begins one's study in 1996, and this technology reaches its maturity at present, and little by little was that vast tobacco grower accepts.Because the variation of seedling raising manners, flue-cured tobacco seedling-cultivating is more and more higher to the requirement of germination rate, emergence rate, speed of emergence and the regularity of flue-cured tobacco pelleted seed.Floating seedlings generally adopts pelleted seed, and still, existing pelleted seed still exists obvious defects, such as must sow effectively cracking under the open fire condition; Also the ubiquity seedling-growing time is long, and emergence rate is lower, it is irregular to emerge, big seedling phenomenon.Thereby, how improving the vigor of tobacco seed-coating kind, this is that tobacco business needs constantly research and one of important topic of exploring.

Summary of the invention

The objective of the invention is at the deficiencies in the prior art part, a production method that grows tobacco vernalization type coat pelleted seeds is provided.

Purpose of the present invention is achieved by following technical proposals.

The invention provides a production method that grows tobacco vernalization type coat pelleted seeds, this method may further comprise the steps:

(1) sterilization: the elite seed quality reaches the tobacco seed of one-level breeding standard, puts into 0.5% copper sulfate solution and soaks 15～20 minutes, and it is clean with flushing with clean water to take out the back, dries standby;

(2) vernalization is prepared: the tobacco seed that will sterilize, and put into distilled water bottle and add running water, then distilled water bottle is placed in the phytotron, and ventilates in bottle with air compressor immediately, throughput is 16～22L/ branch; Delivery port is encased with double-deck clean gauze;

(3) vernalization: germination temperature is 25 ℃～28 ℃, and the phytotron light application time is light/dark replacing respectively 12 hours during vernalization, and germination time is 30～40 hours; When seeing most of seed kind skins when just broken, be about to seed and from distilled water bottle, take out, 28 ℃～30 ℃ following dry for standby;

(4) the dressing Pelleting of seed: with the tobacco seed of vernalization routinely technology through nucleation, Pelleting, typing, polishing, dyeing is oven dry also, promptly obtains required tobacco vernalization type coat pelleted seeds; Wherein used kind of clothing agent raw materials in part by weight is mineral clay 26～32.5, talcum powder 60～70,98% carbendazim original powder, 0.5～1.5,9,0%0,000 clever former powder 0.5～1.0, tobacco compound fertilizer 3～5.

Compared with prior art, the present invention has following beneficial effect:

1. seed is lived organism.The vigor of tobacco seed is that growth, maturation, the storage along with seed constantly changes, and always when its physiological ripening, it is the highest that seed vitality reaches for seed, and vigor takes place to descend in various degree subsequently.Behind seed collection, holding conditions with broadcast before preliminary treatment be even more important.Preliminary treatment can obviously improve the vigor of seed before broadcasting, and makes after planting to emerge neatly the seedling stalwartness.Pretreated method was a lot of before seed was broadcast, and the vernalization technology that the present invention adopts is a kind of simple and effective method.

2. the present invention is in conjunction with existing tobacco seed-coating pill-type technology, and tobacco seed is carried out after the vernalization under the certain condition dressing more earlier, produces the tobacco vernalization type coat pelleted seeds of high vigor.How controlling germination time is the key of this technology, the present invention to tobacco seed germination time and dressing after seed vitality relation further investigate.Studies show that the vernalization of tobacco seed being carried out 30 to 40 hours, dressing just can obviously improve the germination rate of flue-cured tobacco pelleted seed behind the Hui Gan.Dressing behind application vernalization-Hui Gan (being moisture absorption-time dry treatment) is a kind of simple and easy to do, the low cost method that improves the tobacco seed vigor,

(3) by improving kind of the prescription of clothing agent, improve the nutrition of cigarette strain, improve the nutrition resistance and the diseases prevention insect protected ability of cigarette seedling; Also can eliminate seed institute disease carrying germ simultaneously, improve seed vitality, transfer seed self anti-epidemic system by the pre-treatment of coat pelleted seeds.The present invention especially is fit to the floating seedlings mode and uses, and has broad application prospects.

Description of drawings

Fig. 1 is that different germination times are handled potentiality of seed variation schematic diagram;

Fig. 2 is that different germination times are handled percentage of seedgermination variation schematic diagram;

Fig. 3 is vernalization pelleted seed and the conventional pelleted seed schematic diagram that influences to emergence rate;

Fig. 4 is vernalization pelleted seed and conventional pelleted seed leaf fresh weight change curve;

Fig. 5 is vernalization pelleted seed and conventional pelleted seed leaf dry weight change curve;

Fig. 6 is vernalization pelleted seed and conventional pelleted seed root fresh weight change curve;

Fig. 7 is vernalization pelleted seed and conventional pelleted seed root dry weight change curve;

Fig. 8 is vernalization pelleted seed and the conventional pelleted seed schematic diagram to the root system effect of vigor;

Fig. 9 is the situation of emerging of the 14th day vernalization pelleted seed after planting;

Figure 10 is after planting the 14th day conventional pelleted seed situation of emerging;

Figure 11 is the situation of emerging of the 14th day vernalization cloud and mist 85 pelleted seeds after planting;

Figure 12 is the situation of emerging of the 14th day conventional cloud and mist 85 pelleted seeds after planting.

Embodiment

By specific embodiment given below and typical Application Example, can further be well understood to the present invention.But they are not limitation of the invention.

Embodiment 1

The elite seed quality reaches seed 4000 gram of one-level breeding standard, is distributed into several bags, puts into 0.5% copper sulfate solution and soaks 15～20 minutes.It is clean with flushing with clean water to take out the back, dries standby; Seed with having sterilized is sub-packed in the distilled water bottle, and adds running water; The distilled water bottle that seed and water are housed is put into phytotron, and ventilate in bottle with air compressor immediately, at last delivery port is encased with double-deck clean gauze, prevent that extraneous bacterium from entering, pollute seed.Every bottle of throughput is the 20L/ branch.Germination temperature is 25 ℃～28 ℃, and the phytotron light application time is light/dark replacing respectively 12 hours during vernalization.Experimental cultivar is flue-cured tobacco K326 one-level breeding (producing Yuxi, the place of production in 1999).Seed is at first with 0.5% copper sulphate sterilization 15min, with the distilled water flushing several, again respectively at

vernalization

15h, 20h, 25h, 30h, 35h, 40h, 44h, 46h, 48h under the room temperature, with the contrast of doing of not vernalization.Dressing again behind the seed Hui Gan.Each is handled selected 100 pelleted seeds and puts into the culture dish (diameter 10cm) that is lined with double-deck filter paper, with distilled water moistening filter paper, with 10 * 10 at checkrow planting flue-cured tobacco pelleted seed, insert and do sprouting test in the climatic cabinate, temperature is 28 ℃～30 ℃.3 repetitions are established in each processing.

Vitality test: add up germination rate day by day, added up germination vigor and germination rate respectively in 7 days, 14 days, and calculate germination index.Carry out statistical disposition at last.

Subnumber * 100% is planted experimentally in normal germinative number/confession in the germination vigor (%)=7 day

Subnumber * 100% is planted experimentally in normal germinative number/confession in the germination rate (%)=14 day

Germination index=∑ Gt/Dt (Gt is the germinative number in the t time, and Dt is the germination fate of corresponding fate);

Result and analysis:

(1) variance analysis of the capsuled seed vigor of different germination times processing: the tobacco scientific research statistical analysis software that test data is used the exploitation of Kunming mesa scientific and technological development company carries out variance analysis.Each handles the 7th day germination vigor arithmetic equal value between 84.0～94.0%, and the mean value of germination rate is distributed between 84.0～94.0%.Carry out ANOVA showed significant with germination time as single-factor, all there was the significant difference on 1% and 5% level in day germination grain number of the capsuled seed of different disposal from the 2nd day to the 14th day of test between contrast (the vernalization processing time is 0 hour) and different time processing.And the significance of difference between different disposal between different germination fates in some variations, at the 3rd day, except that processing in 15 hours and all the other processing are slightly variant, all the other 8 processing do not have significant difference on 1% level, the 5th day to 6 days between, the significance of difference then shows as, each is all variant between handling and contrasting, there is not difference on 1% level between 5 processing between 15h～35h, there is not difference on 1% level between 4 processing between 40h～48h yet, but has the significant difference of 1% level between the processing of two of 5 processing between 15h～35h and 40h～44h.And after the 7th day, there were significant differences in performance between 44h processing and all the other each processing.By the result of variance analysis as can be known, tobacco seed is carried out vernalization handle and obviously to improve seed germination rate, particularly improved potentiality of seed.Between handling, different germination times has certain difference.Different germination times are handled a day germination rate variance analysis and are seen Table 1.

The different germination times of table 1 are handled a day germination rate variance analysis table

Project		Handle
		Handle	0h 15h 20h 25h 30h 35h 40h 44h
		46h	0h 15h 20h 25h 30h 35h 40h 44h
48h		46h
48h	2 days	Remarkable 5% level 1% of mean value	0.00 2.00 1.33 0.00 0.00 6.33 6.33 2.33 4.00 1.66 c bc bc c a ab ab bc abc bc B AB AB B A AB AB AB AB AB
3 days	2 days	Remarkable 5% level 1% of mean value		Remarkable 5% level 1% of mean value	65.33 75.00 78.67 77.33 80.33 81.67 82.00 87.00 81.66 84.00 c b ab b ab ab ab a ab ab B AB A A A A A A A A
3 days	4 days	Remarkable 5% level 1% of mean value	76.67 81.33 81.00 84.67 85.00 86.00 88.67 92.33 88.00 88.33 c bc bc abc abc ab ab a ab ab B AB AB AB AB AB AB A AB AB	Remarkable 5% level 1% of mean value
	4 days	Remarkable 5% level 1% of mean value		Mean value	82.00 83.00 86.67 86.33 89.67 89.00 92.00 93.33 91.33 89.67

5 days	Remarkable 5% level 1%	c B	c B	bc AB	bc AB	ab AB	ab AB	ab A	a A	ab A	ab AB
5 days	Remarkable 5% level 1%	c B	c B	bc AB	bc AB	ab AB	ab AB	ab A	a A	ab A	ab AB	6 days	Remarkable 5% level 1% of mean value	82.33 c B	86.33 bc AB	87.67 ab AB	88.00 abc AB	89.00 ab AB	90.00 ab AB	92.00 ab A	94.00 a A	91.33 ab A	90.33 ab A
7 days	Remarkable 5% level 1% of mean value	84.00 d B	87.00 cd AB	88.00 bcd AB	89.67 abc AB	90.67 abc AB	90.00 abc AB	92.00 abc A	94.00 a A	91.33 abc AB	90.33 ab AB	6 days	Remarkable 5% level 1% of mean value	82.33 c B	86.33 bc AB	87.67 ab AB	88.00 abc AB	89.00 ab AB	90.00 ab AB	92.00 ab A	94.00 a A	91.33 ab A	90.33 ab A
7 days	Remarkable 5% level 1% of mean value	84.00 d B	87.00 cd AB	88.00 bcd AB	89.67 abc AB	90.67 abc AB	90.00 abc AB	92.00 abc A	94.00 a A	91.33 abc AB	90.33 ab AB	8 days	Remarkable 5% level 1% of mean value	84.33 c B	87.33 bc AB	88.33 abc AB	89.67 abc AB	90.67 ab AB	90.33 ab AB	92.00 ab AB	94.00 a A	91.33 ab AB	90.33 a AB
9 days	Remarkable 5% level 1% of mean value	84.33 c B	87.33 bc AB	88.33 abc AB	89.67 abc AB	90.67 ab AB	90.33 ab AB	92.00 ab AB	94.00 a A	91.33 ab AB	90.33 a AB	8 days	Remarkable 5% level 1% of mean value	84.33 c B	87.33 bc AB	88.33 abc AB	89.67 abc AB	90.67 ab AB	90.33 ab AB	92.00 ab AB	94.00 a A	91.33 ab AB	90.33 a AB
9 days	Remarkable 5% level 1% of mean value	84.33 c B	87.33 bc AB	88.33 abc AB	89.67 abc AB	90.67 ab AB	90.33 ab AB	92.00 ab AB	94.00 a A	91.33 ab AB	90.33 a AB	10 days	Remarkable 5% level 1% of mean value	84.33 c B	87.33 bc AB	88.33 abc AB	89.67 abc AB	90.67 ab AB	90.33 ab AB	92.00 ab AB	94.00 a A	91.33 ab AB	90.33 a AB

Illustrate: the result was identical with the 10th day in the 11st to 14 day, no longer listed.

Embodiment 2

Plant the preparation of clothing agent: 30 kilograms of talcum powder are added in the mixers, add 30 kilograms of mineral clays then, 1 kilogram of 98% carbendazim original powder, 0.5 kilogram of 9,0%0,000 clever former powder, 3.5 the kilogram tobacco compound fertilizer adds remaining 35 kilograms of talcum powder in the mixer at last.Start the machine, mix after 20 minutes, shut down, be required kind clothing agent.

Embodiment 3

Preparation before the dressing:

A. before the seed pelleting, check earlier whether dressing machine inwall is clean, and whether power supply is connected, and whether normally moves; Check whether operations such as Other related equipment such as drying plant, sprayer, packing machine are normal.

B. the preparation of nutrient solution is as shown in table 2:

Table 2.

Stock solution	1 liter of nutrient solution contains the milliliter number of stock solution
Stock solution		1M nitrate of lime	5 * 2
1M potassium nitrate	5 * 2	1M nitrate of lime	5 * 2
1M potassium nitrate	5 * 2	1M magnesium sulfate	2 * 2
The 1M potassium dihydrogen phosphate	1 * 2	1M magnesium sulfate	2 * 2
The 1M potassium dihydrogen phosphate	1 * 2	Fe-EDTA	1 * 2
Micro-nutrient	1 * 2	Fe-EDTA	1 * 2
Micro-nutrient	1 * 2	Water	970
Fe-EDTA: the FeSO4.7H2O of 0.56g is dissolved in the water of 20ml, and the Na-EDTA of 0.75g is dissolved in the hot water of 20ml, behind two kinds of solution mixings, is settled in the volumetric flask of 100ml.Micro-nutrient: the gram number that contains in 1 liter of stock solution: the pH values of 1.81 gram manganese chlorides, 0.11 gram zinc chloride, 0.05 gram copper chloride, 0.25 gram molybdenum sodium, 2.86 gram boric acid nutrient solutions are adjusted between the 5.5-6.5.		Water	970

C. the tobacco seed of vernalization technology coatings Pelleting routinely: will through the tobacco seed of vernalization routinely technology through nucleation, Pelleting, its coating method hockets for spraying nutrient solution and dressing auxiliary material; Typing, polishing, dyeing and oven dry promptly obtain required tobacco vernalization type coat pelleted seeds.

Embodiment 4

Polishing.Tobacco vernalization type coat pelleted seeds after sieving is put into dressing machine branch and is added 8 kilograms of talcum powder three times, carries out polishing in 5～10 minutes at every turn.

Embodiment 5

Dyeing.With the tobacco vernalization type coat pelleted seeds after the polishing, add 100～400 gram dyestuffs, dye in the disposable adding dressing machine.

Embodiment 6

With the tobacco vernalization type coat pelleted seeds after the dyeing, to put into roasting despot immediately and dry, temperature is controlled in 45 ℃, can take out after the oven dry.Tobacco vernalization type coat pelleted seeds after the oven dry carries out classification with the sieve of different meshes by certain specification, obtains required tobacco vernalization type coat pelleted seeds.With the coat pelleted seeds quantitative package of minute crossing level, the vanning of packing back, warehouse-in.

Application Example 1

Germination time and potentiality of seed.As can be seen from Figure 1, the germination vigor of each vernalization processing is all compared according to high.Seed is described after vernalization is handled, seed vitality is improved.The growth potentiality of seed that the relation of germination vigor and germination time shows as generally along with germination time is also increasing, a little peak value appearred in 30 hours in vernalization, but the peak is not obvious, and peak-peak appears in vernalization 44h germination vigor, and germination vigor then reduces along with the prolongation of germination time subsequently.By the result as can be seen, for tobacco seed, vernalization processing time scope can both improve the germination vigor of seed greatly between 30h～46h.

Application Example 2

The germination rate of the tobacco seed that different germination times are handled changes sees Fig. 2.The germination rate that each vernalization is handled is all compared according to high.The growth percentage of seedgermination that the relation of germination rate and germination time shows as generally along with germination time is also increasing, and the peak value of germination rate appears at the processing of vernalization 44h, and germination rate then reduces along with the prolongation of germination time subsequently.As seen from Figure 2, for tobacco seed, vernalization processing time scope can both improve seed germination rate between 30h～46h.It is basic identical with the variation tendency of the variation tendency of germination time and germination vigor to manage germination rate everywhere.

Application Example 3

Germination time and germination index.Put down in writing the chitting piece number every day behind the seed sprouting, and the summation of germinative number every day after divided by corresponding germination fate is germination index, its reflection be the early stage germination number of seed.In general, strong vigor seed is sprouted rapidly and neat, and the germination index of seed becomes positive correlation with the vigor of this seed.As can be seen from Table 3, the seed sprouting index that each vernalization is handled all is higher than contrast, illustrates that vernalization can obviously improve the sprouting speed of tobacco seed.Germination index is up to 44h and handles, and secondly comparison is two processing of 35h, 40h according to exceeding 19.29.Each handles the germination index explanation of tobacco seed, and germination time can obtain higher germination index in 35～44h scope, and the vigor of seed is significantly improved.

The germination index that each vernalization of table 3 is handled

Application Example 4

Tested in 2000 and carry out in Yunnan Inst. of Tobacco Sciences agricultural institute base.Seed is carried out two kinds of processing, and control treatment is that seed is carried out conventional dressing, and another processing is to dry after 35 hours 25 ℃～28 ℃ following vernalization, carries out conventional dressing again.Four parts of pelleted seeds are sowed at respectively in the floating dish, and three repetitions are established in one of every hole sowing.Floating pond specification is 133 * 445cm, and floating dish specification is 66.5 * 34cm, and the nursery pond height is 20cm, depth of water 10cm, and 2 layers of polyethylene films are spread in the bottom, and matrix is for being raw material matrix prepared in proportion with the peat composed of rotten mosses, corn stalk, expanded perlite, raise seedling in greenhouse.Test fertilization time, method and management are carried out according to a conventional method.Each processing of testing index and method is added up seedling-growing time respectively (by the tobacco business standard YC/T 142-1998 of the People's Republic of China (PRC), overall 50% seedling cotyledon is the open and flat date fully), emergence rate, the respectively sub-balance weighing of electricity consumption root system, blade dry weight and fresh weight of plant seedlings at after planting 35 days, 50 days, 65 days, the root system vigor adopts the red tetrazolium method.Measured with ruler that plant height, the stem of cigarette seedling is long, root is long respectively in the 50th day, 65 days and the 5th leaf and leaf is long, leaf is wide.Vernalization pelleted seed and conventional pelleted seed are to the influence of the floating seedling cultivation of fire-cured tobacco emergence rate and the fate of emerging.As can be seen from Figure 3, the preferably whole emergence rate of the vernalization pelleted seed of flue-cured tobacco cloud and mist 85, K326 is higher than conventional pelleted seed in floating seedlings.The early stage (11d) vernalization pelleted seed emergence rate apparently higher than conventional pelleted seed, the emergence rate of the vernalization pelleted seed of cloud and mist 85 is 81.7%, the emergence rate of conventional pelleted seed is 24.8%, and the emergence rate of the vernalization pelleted seed of cloud and mist 85 is 3.3 times of conventional pelleted seed; The emergence rate of K326 vernalization pelleted seed is 54.2%, and the emergence rate of conventional pelleted seed is 20.7%, and the emergence rate of K326 vernalization pelleted seed is 2.6 times of conventional pelleted seed; The emergence rate of two kinds is slightly high with cloud and mist 85, and this may be relevant with the seed vitality genotypic difference of each kind, but two kinds have all obviously improved speed of emergence after vernalization is handled; During to 18d, the emergence rate of vernalization pelleted seed and conventional pelleted seed all reaches higher level; The emergence rate of two types of pelleted seeds changes less afterwards.Final emergence rate remains the vernalization pelleted seed and is higher than conventional pelleted seed.From the whole variation process of emergence rate, the germination period of vernalization pelleted seed mainly concentrates in the 11d-15d, and conventional sprouting of planting pelleted seed then mainly concentrates in the 15d-18d.At the 11st day, the emergence rate of vernalization cloud and mist 85 accounted for 86.6% of final emergence rate, and conventional pelleted seed only accounts for 28.8% of its final emergence rate.This explanation vernalization pelleted seed can significantly improve the emergence rate of floating seedlings cigarette seedling, and to improving speed and the regularity that the flue-cured tobacco seedling emerges, there is effect preferably aspects such as shortening seedling-growing time especially.Seeding stage to the floating seedlings of each material is investigated, and the fate of emerging of vernalization K326 is 10 days, and the conventional pelleted seed K326 fate of emerging is 13 days; The fate of emerging of vernalization cloud and mist 85 is 9 days, and the fate of emerging of conventional pelleted seed is 12 days.The conventional pelleted seed of vernalization pelleted seed average specific was emerged in about 3 days in advance.

Application Example 5

Vernalization pelleted seed and conventional pelleted seed are to becoming the influence of cigarette seedling botany proterties in seedling stage.When 65 days seedling ages, the botany proterties that plant height, stem are long, leaf is long, leaf is wide of vernalization pelleted seed cigarette seedling and conventional pelleted seed cigarette seedling under the mensuration floating seedlings mode.As shown in Table 4, vernalization pelleted seed and conventional pelleted seed become seedling stage botany proterties that certain influence is arranged to floating seedling cultivation of fire-cured tobacco.The cigarette seedling of two flue-cured tobacco cultivars vernalization of cloud and mist 85 and K326 pelleted seed all is higher than conventional pelleted seed in several proterties such as becoming the plant height in seedling stage, stem length, root length, leaf length, Ye Kuan.This explanation vernalization pelleted seed can increase the plant height of cigarette seedling, makes the stem elongation, promotes growing of root system.

Table 4 vernalization pelleted seed and conventional pelleted seed are to becoming the influence (unit: cm) of cigarette seedling botany proterties in seedling stage

Handle	Plant height	Stem is long	Root is long	Leaf is long	Leaf is wide
Handle	Plant height	Stem is long	Root is long	Leaf is long	Leaf is wide	Vernalization K326	33.10	14.95	7.48	21.21	6.47
Conventional K326	32.15	14.33	7.33	20.65	6.42	Vernalization K326	33.10	14.95	7.48	21.21	6.47
Conventional K326	32.15	14.33	7.33	20.65	6.42	Vernalization cloud and mist 85	37.82	20.97	8.22	20.62	6.58
Conventional cloud and mist 85	35.22	19.90	7.63	20.33	6.2	Vernalization cloud and mist 85	37.82	20.97	8.22	20.62	6.58

Application Example 6

Vernalization pelleted seed and conventional pelleted seed are to the tired influence of material knot in the tobacco seedling growth process.By Fig. 4, Fig. 5 as can be seen, the leaf fresh weight of cloud and mist 85 and two kind cigarettes of K326 seedling, leaf dry weight are in whole seedling growth process, all show as the vernalization pelleted seed and be higher than conventional pelleted seed, cigarette seedling aerial growth speed is described, the vernalization pelleted seed is faster than conventional pelleted seed; From Fig. 6, Fig. 7 as can be seen, two kind cigarette shoot roots system be grown in incipient stage vernalization pelleted seed and conventional pelleted seed does not have obvious difference, but after emerging back 35 days, the root aquatic foods of vernalization pelleted seed, dry weight are apparently higher than conventional pelleted seed, and cigarette seedling underground part growth rate vernalization pelleted seed is faster than conventional pelleted seed.No matter be the substance accumulation amount of overground part or underground part, the cigarette seedling of vernalization pelleted seed is all apparently higher than the cigarette seedling of conventional pelleted seed.

Application Example 7

The vernalization pelleted seed is the influence of vigor to floating seedling cultivation of fire-cured tobacco cigarette shoot root.Root system is the vitals that tobacco absorbs moisture, nutrient, is again synthetic position such as amino acid and nicotine.The growing state of root system and activity level directly influence the g and D of cigarette strain acrial part, and then influence the quality of tobacco leaf.As seen from Figure 8, after emerging 28 days, 35 days, the root system vigor of cloud and mist 85, two kind vernalization of K326 pelleted seed is a little less than conventional pelleted seed, after 42 days after emerge, the cigarette shoot root of two kind vernalization pelleted seeds is that vigor rises rapidly, obviously surpasses the root system vigor of conventional pelleted seed.Illustrate that the vernalization pelleted seed has tangible increase effect to the root system of floating seedlings cigarette seedling.

Claims

1. a production method that grows tobacco vernalization type coat pelleted seeds, this method may further comprise the steps:

(1) sterilization: the elite seed quality reaches the tobacco seed of one-level breeding standard, soaks in 0.5% the copper sulfate solution 15～20 minutes, and is clean with flushing with clean water after taking out, and dries standby;

(2) vernalization is prepared: the tobacco seed that will sterilize, put into the distilled water bottle that running water is housed, be placed in the phytotron then, and ventilate in bottle with air compressor immediately, throughput is 16L/ branch～20L/ branch, and delivery port encases with double-deck clean gauze;

(3) vernalization: germination temperature is 25 ℃～28 ℃, and the phytotron light application time is light/dark replacing respectively 12 hours during vernalization; Germination time is 30～40 hours; When seeing most of seed kind skins when just broken, be about to seed and from distilled water bottle, take out and dry, bake out temperature is 28 ℃～30 ℃, and is standby;

(4) the dressing Pelleting of seed: use kind of clothing agent with the tobacco seed of vernalization routinely technology promptly obtain required tobacco vernalization type coat pelleted seeds through nucleation, Pelleting, typing, polishing, dyeing and oven dry.

2. the production method of tobacco vernalization type coat pelleted seeds according to claim 1, wherein the raw materials in part by weight of used kind of clothing agent is in step (4): mineral clay 26～32.5, talcum powder 60～70,98% carbendazim original powder 0.5～1.5,9,0%0,000 clever former powder 0.5～1.0, tobacco compound fertilizer 3～5.