CN1220426C - Method for reproducing North Ameria tulip trees by using transplanting cuttings - Google Patents
Method for reproducing North Ameria tulip trees by using transplanting cuttings Download PDFInfo
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- CN1220426C CN1220426C CN 200410006339 CN200410006339A CN1220426C CN 1220426 C CN1220426 C CN 1220426C CN 200410006339 CN200410006339 CN 200410006339 CN 200410006339 A CN200410006339 A CN 200410006339A CN 1220426 C CN1220426 C CN 1220426C
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Abstract
The present invention belongs to a combination technique for the propagation of forest trees by using transplanting cuttings, which provides a method particularly to for the propagation of northern American tulip trees by using transplanting cuttings, and an antiseptic root-growing paste membrane. The present invention is suitable for the propagation of China liriodendron. The present invention solves the defects of long cutting rooting time for northern American tulip trees, putrescibility of spikes, etc. existing in the prior art by the reasonable combination of spike selecting, culture field configuration, antiseptic root-growing paste membrane recipe and spraying moisture management. The present invention provides a recipe and a propagation method which can prevent cutting spikes from decaying and accelerate rooting, and the survival rate for repeated cutting woods is more than 85%.
Description
[technical field]
The invention belongs to the combination technique that is propagated from cuttings of forest, specifically provide a kind of yellow poplar method that is propagated from cuttings, provide the anti-saprophytic root of a kind of supporting otch to stick with paste film simultaneously.The present invention also is applicable to the breeding of Chinese mandarin jacket wood.
[background technology]
Yellow poplar (Liriodendron tulipifera L.) natural distribution is in the Eastern section of the U.S and the Canadian southeast, and mainly concentrate on the A Balaqiya mountain area, become East Asia with the Chinese mandarin jacket wood that belongs to together (L.chinense (Hemsl.) sarg) and two kinds that distribute are interrupted in North America.Equally, yellow poplar (Liriodendron) material is good, blade profile is peculiar, spend attractive in appearance greatly, the tree height is big, strong stress resistance is that warm type weather epoch Oligocene epoch origin back develops and the ancient type plant that comes, and unique at aspects such as afforestation, industrial cut stock, improvements of the ecological environment have a higher value.China's mandarin jacket wood is Chinese Second Class Key Protected Plant, and the present is distributed in the middle and south of China, and yellow poplar then only is distributed in each state, the central and east of the U.S., and natural resources slightly enrich degree and are higher than Chinese mandarin jacket wood.
The inventor scale is arranged first introduces a fine variety 5 yellow poplar south provenances in nineteen ninety, and 5 provinces have carried out the multiple spot afforestation experiment that comprises 15 Chinese mandarin jacket wood provenances in the middle and lower reach of Yangtze River.1994, introduce 4 yellow poplar central and north provenances, carried out growing seedlings and afforestation experiment in 3 places such as Beijing.1999, under the support of " 948 " project, go to U.S.A. in person fact-finding and seed collection, successively gathered the experiment material of 14 central and north provenances altogether, and in Beijing, Dalian, grow seedlings and afforest in ground such as Zhengzhou, by the amount of growth research and appraisal, the storage rate research and appraisal, wood property is analyzed and the RAPD evaluation of markers is analyzed, be China different regions, particularly to have selected adaptability strong in the North China, amount of growth is big, material is good, fine provenance with genetic characteristics is further bred for it and to be promoted and the apolegamy of hybrid strain etc. provides scientific basis.
Yellow poplar seed cost an arm and a leg (market price general 20 yuan/gram), germination rate very low (general 3～5%), influence quick breeding of these seeds and applying of improved seeds, restrict it and introduced a fine variety the development in place, at present therefore, research and solve the fine provenance of these seeds and select and the cuttage root-taking technology, have important production practices meaning and value.Studies show that both at home and abroad, Chinese tulip tree vegetative propagation difficulty, belong to the woody plant that difficulty is taken root, famous vegetative propagation expert Wang Tao (Wang Tao, 1989, plant cuttage propagation technique, Beijing science tech publishing house) with homemade root-inducing powder to Chinese tulip tree do also to conclude after the test the hard branch of Chinese tulip tree take root extremely difficult.Though the not high small plot experiment report of a small amount of cuttage root-taking is arranged, as (Chen Shiqun and Yang Yonglan etc., 1993, Chongqing forestry science and technology, 36 (1～4):, only obtain 3% root survival such as Chen Shiqun 30～33) through test for several times.Mostly be the result of the test of indoors artificial environment wherein, more fail the formation scale, do not see the asexual fast numerous report that puts into production and utilize, so production practices have little significance.
In the U.S., though see the report (MerkleS.A. that the full mature embryo that toos many or too much for use carries out tissue-culturing rapid propagation, et al., Plant Cell, Tissue and Organ Culture, 1993,34:192～198), but utilize incomplete mature embryo to carry out the nursery stock that tissue-culturing rapid propagation goes out, exist genetic background unclear, can't guarantee merit and cost expensive and must etc. the long period to blossoming and bearing fruit shortcomings such as just can obtaining propagating materials.Therefore, yellow poplar vegetative propagation difficulty has become to restrict these seeds and the elite germplasm material is bred the technical bottleneck of applying in a large number in China.
The inventor tests accumulation and other people relevant report according to the cuttage of multiple years in the past, long at the yellow poplar cuttage root-taking time, characteristics such as the fringe bar is perishable, pass through repetition test, found out a cover and can prevent that plugged ear is rotten, prescription that acceleration is taken root and treatment measures, and under outdoor environmental conditions, obtained breakthrough success.Of the present inventionly successfully provide service for a large amount of vegetative propagations of yellow poplar improved seeds and application aborning thereof.
[summary of the invention]
[technical problem that will solve]
Shortcomings such as it is long to the invention solves the yellow poplar cuttage root-taking time that exists in the prior art, and the fringe bar is perishable provide a cover can prevent that plugged ear from rotting, quickening the prescription and the propagation method of taking root.
[technical scheme]
The invention provides a kind of economical and practical yellow poplar method that is propagated from cuttings, stick with paste the incision of film formulation parcel cuttage branch in the described method with anti-saprophytic root.This method specifically is the reasonable combination that the fringe bar is selected, training ground disposes, anti-saprophytic root is stuck with paste film parcel and spraying and moisturizing management.
Wherein said fringe bar is selected 1～3 year living sprout.Be preferably 1～3 year living sprout and have the sprout of terminal bud.
Matrix in the wherein said training ground configuration is selected fresh pine sawdust or is mixed 15% perlite peat soil.
Wherein said spraying and moisturizing management was sprayed water general 1 minute for fine per about 1 hour, and 1 water of per 2～3 hours of cloudy day spray stops water spray night.
It is to contain in 1 kilogram of anti-saprophytic root paste film formulation that the employed anti-saprophytic root of the method that is propagated from cuttings provided by the invention is stuck with paste the film formulation component: 400～600 gram talcum powder; 0.4～0.6 gram root-inducing powder; 20～30 gram carbendazim; Surplus is a water.
The method that is propagated from cuttings of the present invention realizes by following step:
1) prepares matrix, sprayer unit, slotting machine;
2) prepare fringe bar: 10～15cm, truncation or horseshoe incision, band terminal bud;
3) prepare the anti-saprophytic root of otch and stick with paste the film formulation prescription, can use the small container packing after preparing;
4) cuttage: the first step, with small wooden perforating on slotting machine, the degree of depth 4～8cm, the eye column pitch is from 7-10cm * 7-10cm; Second step, get and shear good fringe bar, otch is put into anticorrosion pastel prescription, allow apart from the about 2～5cm section of notch all be wrapped by pastel; In the 3rd step, the fringe bar that wraps notch is put into eye position on the slotting machine, the degree of depth 3～5cm gently; The 4th step, the matrix around the compacting fringe bar; In the 5th step, start sprayer unit.
The above-mentioned method that is propagated from cuttings, it is applicable to being propagated from cuttings of Chinese mandarin jacket wood and hybrid thereof.
The present invention also provides the anti-saprophytic root of a kind of otch to stick with paste film, and its component is to contain in 1 kilogram of anti-saprophytic root paste film formulation: 400～600 gram talcum powder; 0.4～0.6 gram root-inducing powder; 20～30 gram carbendazim; Surplus is a water.Above-mentioned anti-saprophytic root is stuck with paste film, and it is applicable to that yellow poplar, Chinese mandarin jacket wood are propagated from cuttings.
[beneficial effect]
Grope research to what yellow poplar cuttage test had been carried out reaching surplus in the of 10 year, summed up a branch cuttage combination technique, comprise that the fringe bar is selected, the configuration of training ground, anti-saprophytic root stick with paste film parcel and spraying and moisturizing management etc.Calendar year 2001 has obtained in batches first, and cottage propagation surpasses 85% root survival.2002 3 times repeated test shows, after having adopted the method that is propagated from cuttings provided by the invention, the asexual cuttage of Chinese tulip tree no longer becomes a difficult problem, it is very high to be inserted into motility rate (taking root+callus) its summer before the spray, the highest sub-district can reach 100%, combination average can reach more than 93% preferably, and the cuttage test also can reach about 88%, near 90% in batches.The fringe bar age the analysis showed that, the fringe bar of giving birth to children's seedling in age in 1～3 year is better than the fringe bar of gathering in life in 4 years or the more above the average age for marriage one-tenth strain.Test finds, yellow poplar has that hole skin is taken root and callus is taken root two types, and the amount of taking root of two types is similar.The field-transplanting survival rate of yellow poplar cuttage root-taking seedling is higher, reaches more than 85%.
Data of the present invention show that the asexual cottage propagation of yellow poplar has obtained the novelty breakthrough, for the expanding propagation and the utilization and extention of this seeds fine provenance provides powerful technical support, have important production practices meaning.
Simultaneously, the matrix that general cottage propagation uses all needs sterilization processing, guarantees that branch is not subjected to bacterial infection; Anti-saprophytic root provided by the invention is stuck with paste film parcel cuttage branch, guarantee that branch is not subjected to bacterial infection, thereby matrix of the present invention does not need sterilization processing, has saved a large amount of costs like this in production application, and is also easy in the operation.According to a large amount of experiment contrasts, preferred fresh pine sawdust of the present invention or mixed 15% perlite peat soil.
[embodiment]
The preparation 1 that embodiment 1, the anti-saprophytic root of otch are stuck with paste film:
Take by weighing 400 and restrain talcum powder, 0.4 gram root-inducing powder, 20 gram carbendazim, stir, it is 1 kilogram that injection water makes its gross weight, stirs and can obtain the anti-saprophytic root paste of pasty state otch film.
The preparation 2 that embodiment 2, the anti-saprophytic root of otch are stuck with paste film:
Take by weighing 600 and restrain talcum powder, 0.6 gram root-inducing powder, 30 gram carbendazim, stir, it is 1 kilogram that injection water makes its gross weight, stirs and can obtain the anti-saprophytic root paste of pasty state otch film.
The preparation 3 that embodiment 3, the anti-saprophytic root of otch are stuck with paste film:
Take by weighing 500 and restrain talcum powder, 0.5 gram root-inducing powder, 25 gram carbendazim, stir, it is 1 kilogram that injection water makes its gross weight, stirs and can obtain the anti-saprophytic root paste of pasty state otch film.
Embodiment 4, the yellow poplar method that is propagated from cuttings
1) prepares fresh pine sawdust matrix, sprayer unit, slotting machine;
2) prepare fringe bar (mid-July): 10cm, truncation or horseshoe incision;
3) the anti-saprophytic root of otch of preparing embodiment 1 is stuck with paste film, can use the small container packing after preparing;
4) cuttage: the first step, with small wooden perforating on slotting machine, degree of depth 4cm, the eye column pitch is from 7cm * 7cm; Second step, get and shear good fringe bar, otch is put into the anti-saprophytic root of otch stick with paste film, allow apart from the about 2cm section of notch all be wrapped by pastel; In the 3rd step, the fringe bar that wraps notch is put into eye position on the slotting machine, degree of depth 3cm gently; The 4th step, the matrix around the compacting fringe bar; In the 5th step, start sprayer unit.
Embodiment 5, the yellow poplar method that is propagated from cuttings
1) prepares to mix 15% perlite peat soil matrix, sprayer unit, slotting machine;
2) prepare fringe bar (mid-August): 15cm, truncation or horseshoe incision, band terminal bud;
3) the anti-saprophytic root of otch of preparing embodiment 2 is stuck with paste film, can use the small container packing after preparing;
4) cuttage: the first step, with small wooden perforating on slotting machine, degree of depth 6cm, the eye column pitch is from 10cm * 10cm; Second step, get and shear good fringe bar, otch is put into the anti-saprophytic root of otch stick with paste film, allow apart from the about 5cm section of notch all be wrapped by pastel; In the 3rd step, the fringe bar that wraps notch is put into eye position on the slotting machine, degree of depth 5cm gently; The 4th step, the matrix around the compacting fringe bar; In the 5th step, start sprayer unit.
Management after embodiment 6, yellow poplar are transplanted a cutting
Management after the cuttage is that fine day was sprayed water general 1 minute in per about 1 hour, and 1 water of per 2～3 hours of cloudy day spray stops water spray night.
Experimental example 1: scale test
Early August calendar year 2001 Beijing middle forest the earth nursery, cut 2 years living seedling side shoots of yellow poplar, 1000 of cuttages, 893 of (taking root+callus) seedlings (accounting for 89.3%), but the equal transplant survival of take root seedling and callus seedling.
Experimental example 2: industrialization test 1
In mid-August, 2002 Beijing middle forest the earth nursery, cut 3 years living nursery stock side shoots of yellow poplar, 4505 of cuttages, must take root seedling 2742 (accounting for 60.9%), 1205 of callus seedlings (accounting for 26.7%), two add up to 3948 (87.6%), but take root seedling and the equal transplant survival of callus seedling.Two of part sub-districts amount to 100%.
Experimental example 3: industrialization test 2
In mid-July, 2002, the Chinese mandarin jacket wood of life in 2 years seedling side shoot was cut in Beijing middle forest the earth nursery, and 200 of cuttages get 173 of (taking root+callus) seedlings (accounting for 86.5%), and survival rate and yellow poplar are suitable.
Experimental example 4: industrialization test 3
In mid-July, 2002, the Chinese mandarin jacket wood of life in 2 years hybrid seedling side shoot was cut in Beijing middle forest the earth nursery, and 200 of cuttages get 173 of (taking root+callus) seedlings (accounting for 86.5%), and survival rate and yellow poplar are suitable.
Experimental example 5, the statistical analysis of cuttage root-taking time
After 1 week of cuttage, begin the situation of taking root of observation experiment example 2,, do not repeat to extract 10 at every turn, repeat to extract 8 weeks of Continuous Observation 3 times in the observation district of taking root.According to the observational record analysis, 2 years living fringe bars of yellow poplar mix the rootage duration in perlite and two kinds of matrix of pine sawdust and the basically identical of taking root at peat.Callus formed since the 2nd week, the 3rd week began to have a spot of outstanding existing, really sending out roots is that (root is grown 2～5mm since the 4th week, can obviously recognize), the 5th week, the amount of the taking root surge of the 6th week, from the 7th week, the 8th week, the amount of taking root begins to become slow increase.Therefore, the yellow poplar epicormic branch cuttage was taken root since the 4th week, and only a few can advance to for the 3rd week, and the 5th, the 6th week was to take root period in a large number.
Experimental example 6: cuttage root-taking seedling and callus transplantation of seedlings survival rate
On the basis of calendar year 2001 experimental example 1 cuttage success, the Spring Equinox in 2002 other to the cuttage root-taking seedling and produced callus but not the seedling of long root (be called for short callus seedling) transplant respectively.Transplant altogether totally 945,810 of the seedlings of wherein taking root, 135 of callus seedlings, autumn in 2002 was investigated the transplant survival seedling, totally survived 826 of seedlings, and the seedling of wherein taking root survives 764, and the callus seedling survives 62 (table 1).The survival rate of seedling of taking root is 94.3%, is higher than the survival rate 45.9% of callus seedling.Though the survival rate of callus seedling is lower, but nearly 50% have only callus and do not have the cuttage seeding of root system arranged still, in the transplanting in 1 year, survive, the vitality that the Chinese tulip tree cuttage seeding is described is stronger, this application in practice for Chinese tulip tree cuttage root-taking and transplanting provide have important value exploration and foundation.
Table 1 yellow poplar cuttage seeding transplanting survival rate application form
| The cuttage root-taking seedling | Cuttage callus seedling | Summation | |
| Transplant a number and survive a number transplanting survival rate | 810 764 94.3％ | 135 62 45.9％ | 945 826 87.4％ |
Experimental example 7: the mensuration of reproduction coefficient
In order to grasp the reproduction speed of yellow poplar cottage propagation, investigation statistics in 2003 2 years living seedling of experimental example 1 gained the quantity of effective plugged ear is provided at 1 growth Ji Zhongke.According to investigation result, average every living seedling of 2a can provide 6.7 of effective fringe bars season 1 growth.Then reproduction coefficient=fringe bar number (n) * (rooting rate * transplantation of seedlings survival rate of taking root+callus rate * callus transplantation of seedlings survival rate)=6.7 * (66.3% * 94.7%+27.5% * 45.9)=5.05. is the asexual cottage propagation of 1 strain 2a seedling by 1 growth season, can realize on average that the quantity more than 5 times increases, if cutting the fringe bar again with the cottage propagation seedling reuses, then two growths will increase more than 25 times in season, and 4 growths will be expected to be increased to more than 625 times in season.
Claims (6)
1, a kind of yellow poplar method that is propagated from cuttings, it comprises the steps:
(1) the fringe bar is selected;
(2) training ground configuration; Matrix in the described training ground configuration is selected fresh pine sawdust or is mixed 15% perlite peat soil;
(3) stick with paste the film parcel in fringe bar incision with anti-saprophytic root, described anti-saprophytic root is stuck with paste in every kilogram of the film and is contained: 400～600 gram talcum powder; 0.4～0.6 gram root-inducing powder; 20～30 gram carbendazim; Surplus is a water;
(4) spraying and moisturizing management.
2, the method that is propagated from cuttings according to claim 1, wherein said fringe bar is selected 1～3 year living sprout.
3, the method that is propagated from cuttings according to claim 2, wherein said fringe bar selects to have 1～3 year living sprout of terminal bud.
4, the method that is propagated from cuttings according to claim 1, wherein said spraying and moisturizing management was sprayed water general 1 minute for fine per about 1 hour; 1 water of per 2～3 hours of cloudy day spray; Stop water spray night.
5,, it is characterized in that realizing by following step according to the method that is propagated from cuttings of claim 1:
(1) prepares matrix, sprayer unit, slotting machine;
(2) prepare fringe bar: 10～15cm, truncation or horseshoe incision, band terminal bud;
(3) prepare the anti-saprophytic root of otch and stick with paste film, prepare back small container packing;
(4) cuttage: the first step, with small wooden perforating on slotting machine, the degree of depth 4～8cm, the eye column pitch is from 7-10cm * 7-10cm; Second step, get and shear good fringe bar, otch is put into anticorrosion pastel prescription, allow apart from the about 2～5cm section of notch all be wrapped by pastel; In the 3rd step, the fringe bar that wraps notch is put into eye position on the slotting machine, the degree of depth 3～5cm gently; The 4th step, the matrix around the compacting fringe bar; In the 5th step, start sprayer unit.
6, a kind of anti-saprophytic root that is used for the described method that is propagated from cuttings of claim 1 is stuck with paste film, it is characterized in that, described anti-saprophytic root is stuck with paste in every kilogram of the film and contained: 400～600 gram talcum powder; 0.4～0.6 gram root-inducing powder; 20～30 gram carbendazim; Surplus is a water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410006339 CN1220426C (en) | 2004-02-26 | 2004-02-26 | Method for reproducing North Ameria tulip trees by using transplanting cuttings |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410006339 CN1220426C (en) | 2004-02-26 | 2004-02-26 | Method for reproducing North Ameria tulip trees by using transplanting cuttings |
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| CN1220426C true CN1220426C (en) | 2005-09-28 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112178B (en) * | 2007-09-07 | 2011-03-09 | 南京林业大学 | Crossbred Chinese tulip tree clone trophozoite suspension cell line inducement method for the generating and regenerating of somatic embryo |
| CN103947673B (en) * | 2014-04-11 | 2015-10-28 | 山东省潍坊市农业科学院 | Antibacterial root-growing agent of a kind of slowly-releasing and preparation method thereof |
| CN104025854A (en) * | 2014-06-06 | 2014-09-10 | 上海应用技术学院 | Drought resistant cultivation method for tulip tree in gravel soil |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN105993522A (en) * | 2016-05-31 | 2016-10-12 | 宜宾云辰乔木园林有限责任公司 | Method for improving tulip tree cutting rooting survival rate |
| CN105875338B (en) * | 2016-06-05 | 2018-11-30 | 江西省科学院生物资源研究所 | A kind of sub- U.S. Liriodendron chinense method for stunting of potting |
| CN107087493A (en) * | 2017-06-15 | 2017-08-25 | 潍坊职业学院 | A kind of golden-rimmed Liriodendron hardwood cuttage method in U.S. |
| CN109463144A (en) * | 2018-12-27 | 2019-03-15 | 南京八月果农业科技开发有限公司 | A kind of Three Akebia Decne Species cuttage rapid propagation method |
| CN118147343B (en) * | 2024-03-19 | 2024-09-13 | 中国林业科学研究院林业研究所 | Method for high-throughput identification of tulip tree varieties and special KASP primer combination thereof |
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