CN119998441A - 使用温度响应性微载体的细胞培养方法及温度响应性细胞培养用微珠 - Google Patents
使用温度响应性微载体的细胞培养方法及温度响应性细胞培养用微珠 Download PDFInfo
- Publication number
- CN119998441A CN119998441A CN202380070479.3A CN202380070479A CN119998441A CN 119998441 A CN119998441 A CN 119998441A CN 202380070479 A CN202380070479 A CN 202380070479A CN 119998441 A CN119998441 A CN 119998441A
- Authority
- CN
- China
- Prior art keywords
- temperature
- cells
- responsive
- culture
- polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/04—Coating
- C08J7/0427—Coating with only one layer of a composition containing a polymer binder
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
- C08J2325/02—Homopolymers or copolymers of hydrocarbons
- C08J2325/04—Homopolymers or copolymers of styrene
- C08J2325/06—Polystyrene
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2433/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
- C08J2433/06—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing only carbon, hydrogen, and oxygen, the oxygen atom being present only as part of the carboxyl radical
- C08J2433/08—Homopolymers or copolymers of acrylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2433/24—Homopolymers or copolymers of amides or imides
- C08J2433/26—Homopolymers or copolymers of acrylamide or methacrylamide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2539/00—Supports and/or coatings for cell culture characterised by properties
- C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Rheumatology (AREA)
- Developmental Biology & Embryology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Thermal Sciences (AREA)
- Physics & Mathematics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022160143 | 2022-10-04 | ||
| JP2022-160143 | 2022-10-04 | ||
| JP2022160144 | 2022-10-04 | ||
| JP2022-160144 | 2022-10-04 | ||
| JP2023-039008 | 2023-03-13 | ||
| JP2023039008 | 2023-03-13 | ||
| JP2023087922 | 2023-05-29 | ||
| JP2023-087922 | 2023-05-29 | ||
| PCT/JP2023/036049 WO2024075722A1 (ja) | 2022-10-04 | 2023-10-03 | 温度応答性マイクロキャリアを用いた細胞培養方法及び温度応答性細胞培養用ビーズ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN119998441A true CN119998441A (zh) | 2025-05-13 |
Family
ID=90608118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202380070479.3A Pending CN119998441A (zh) | 2022-10-04 | 2023-10-03 | 使用温度响应性微载体的细胞培养方法及温度响应性细胞培养用微珠 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP4600345A1 (https=) |
| JP (1) | JPWO2024075722A1 (https=) |
| KR (1) | KR20250057032A (https=) |
| CN (1) | CN119998441A (https=) |
| WO (1) | WO2024075722A1 (https=) |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012029877A1 (ja) | 2010-08-31 | 2012-03-08 | 株式会社セルシード | 温度応答性細胞培養用ビーズ及びその製造方法 |
| EP2612902B1 (en) | 2010-08-31 | 2016-10-26 | Tokyo Women's Medical University | Temperature-responsive substrate for cell culture and method for producing same |
| JP7127330B2 (ja) | 2017-04-12 | 2022-08-30 | 東ソー株式会社 | ブロック共重合体およびそれを用いた表面処理剤 |
| JP6954047B2 (ja) | 2017-11-22 | 2021-10-27 | 東ソー株式会社 | ブロック共重合体コートビーズおよびその製造方法 |
| JP2019126326A (ja) * | 2018-01-26 | 2019-08-01 | 東ソー株式会社 | 多能性幹細胞の剥離方法 |
| JP2020110140A (ja) * | 2019-01-11 | 2020-07-27 | 東ソー株式会社 | 細胞培養方法 |
| JP2021106543A (ja) | 2019-12-27 | 2021-07-29 | ダイキン工業株式会社 | 温度応答性細胞培養用マイクロキャリア |
| JP7647047B2 (ja) * | 2020-01-21 | 2025-03-18 | 東ソー株式会社 | 低組織因子活性細胞、およびその製法 |
| JP2021159049A (ja) * | 2020-04-03 | 2021-10-11 | ダイキン工業株式会社 | 接着性細胞を増殖させる方法、及びその方法のために用いられるマイクロキャリア |
-
2023
- 2023-10-03 CN CN202380070479.3A patent/CN119998441A/zh active Pending
- 2023-10-03 WO PCT/JP2023/036049 patent/WO2024075722A1/ja not_active Ceased
- 2023-10-03 KR KR1020257010846A patent/KR20250057032A/ko active Pending
- 2023-10-03 EP EP23874849.5A patent/EP4600345A1/en active Pending
- 2023-10-03 JP JP2024555804A patent/JPWO2024075722A1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4600345A1 (en) | 2025-08-13 |
| KR20250057032A (ko) | 2025-04-28 |
| JPWO2024075722A1 (https=) | 2024-04-11 |
| WO2024075722A1 (ja) | 2024-04-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI744586B (zh) | 幹細胞培養用支架材料及使用其之幹細胞培養方法 | |
| Mingyu et al. | Surface modification and characterization of chitosan film blended with poly-L-lysine | |
| US10131874B2 (en) | Cell culture support and associated method for cell growth and release | |
| CN112778543B (zh) | 一种用于肌肉干细胞培养的交联水凝胶的制备方法及应用 | |
| JP2024539956A (ja) | フィーダー細胞としておよび合成抗原提示細胞としてのヒドロゲル粒子 | |
| CN112778544B (zh) | 用于肌肉干细胞培养的交联水凝胶及其制备方法和应用 | |
| Wissemann et al. | Pure gelatin microcarriers: synthesis and use in cell attachment and growth of fibroblast and endothelial cells | |
| Darge et al. | Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing | |
| JP7416185B2 (ja) | 立体的細胞構造体の製造方法 | |
| JP2022126087A (ja) | 細胞培養用担体、及び細胞培養用担体の製造方法 | |
| WO2021085317A1 (ja) | 浮遊培養用培地添加剤、培地組成物及び培養方法 | |
| CN119998441A (zh) | 使用温度响应性微载体的细胞培养方法及温度响应性细胞培养用微珠 | |
| KR20180115531A (ko) | 세포 배양용 셀룰로오스 나노섬유 3차원 구조체의 제조방법 및 그에 따라 제조된 세포 배양용 셀룰로오스 나노섬유 3차원 구조체 | |
| Peters et al. | A new approach for adipose tissue regeneration based on human mesenchymal stem cells in contact to hydrogels—An in vitro study | |
| CN101701207A (zh) | 一种温度敏感角膜上皮细胞培养材料的制备方法与应用 | |
| JP7250248B2 (ja) | ブロック共重合体、それを含む表面処理剤及び膜、並びに、それを用いた細胞培養用器材及び細胞培養方法 | |
| JP2018201463A (ja) | 幹細胞を調製するための方法およびキット | |
| JPWO2024075722A5 (https=) | ||
| JP2025127417A (ja) | 温度応答性マイクロキャリアを用いた拡大培養方法 | |
| RU2800523C2 (ru) | Макроноситель | |
| WO2024253069A1 (ja) | 温度応答性マイクロキャリア | |
| Salazar-García et al. | Hydrogels Synthesis of Alginic Acid as Potential Tissue Regeneration Scaffolds | |
| Sempere et al. | María Noel Tamaño-Machiavello1, Juan Carlos Marín Payá1, Silvia Flores1, Lourdes Cordón2, 3 | |
| KR20250172953A (ko) | 조성물 | |
| CN118359827A (zh) | 一种用于构建体外3d肿瘤类器官模型的水凝胶及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |