KR20250057032A - 온도응답성 마이크로캐리어를 이용한 세포배양방법 및 온도응답성 세포배양용 비드 - Google Patents

온도응답성 마이크로캐리어를 이용한 세포배양방법 및 온도응답성 세포배양용 비드 Download PDF

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KR20250057032A
KR20250057032A KR1020257010846A KR20257010846A KR20250057032A KR 20250057032 A KR20250057032 A KR 20250057032A KR 1020257010846 A KR1020257010846 A KR 1020257010846A KR 20257010846 A KR20257010846 A KR 20257010846A KR 20250057032 A KR20250057032 A KR 20250057032A
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temperature
cells
responsive
culture
polymer
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Korean (ko)
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쇼야 히라토코
유스케 네즈
신야 이마토미
히로유키 이토
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토소가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/0427—Coating with only one layer of a composition containing a polymer binder
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
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    • C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
    • C08J2325/02—Homopolymers or copolymers of hydrocarbons
    • C08J2325/04—Homopolymers or copolymers of styrene
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    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2433/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
    • C08J2433/06—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing only carbon, hydrogen, and oxygen, the oxygen atom being present only as part of the carboxyl radical
    • C08J2433/08—Homopolymers or copolymers of acrylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2433/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2433/24—Homopolymers or copolymers of amides or imides
    • C08J2433/26—Homopolymers or copolymers of acrylamide or methacrylamide
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    • C12N2523/00—Culture process characterised by temperature
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2531/00—Microcarriers
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
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    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Sustainable Development (AREA)
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  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Thermal Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020257010846A 2022-10-04 2023-10-03 온도응답성 마이크로캐리어를 이용한 세포배양방법 및 온도응답성 세포배양용 비드 Pending KR20250057032A (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
JPJP-P-2022-160143 2022-10-04
JP2022160143 2022-10-04
JPJP-P-2022-160144 2022-10-04
JP2022160144 2022-10-04
JP2023039008 2023-03-13
JPJP-P-2023-039008 2023-03-13
JP2023087922 2023-05-29
JPJP-P-2023-087922 2023-05-29
PCT/JP2023/036049 WO2024075722A1 (ja) 2022-10-04 2023-10-03 温度応答性マイクロキャリアを用いた細胞培養方法及び温度応答性細胞培養用ビーズ

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KR20250057032A true KR20250057032A (ko) 2025-04-28

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EP (1) EP4600345A1 (https=)
JP (1) JPWO2024075722A1 (https=)
KR (1) KR20250057032A (https=)
CN (1) CN119998441A (https=)
WO (1) WO2024075722A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6313822B2 (ja) 2010-08-31 2018-04-18 株式会社セルシード 温度応答性細胞培養用ビーズ及びその製造方法
JP2021106543A (ja) 2019-12-27 2021-07-29 ダイキン工業株式会社 温度応答性細胞培養用マイクロキャリア
JP6954047B2 (ja) 2017-11-22 2021-10-27 東ソー株式会社 ブロック共重合体コートビーズおよびその製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012029882A1 (ja) 2010-08-31 2012-03-08 学校法人東京女子医科大学 細胞培養用温度応答性基材及びその製造方法
JP7127330B2 (ja) 2017-04-12 2022-08-30 東ソー株式会社 ブロック共重合体およびそれを用いた表面処理剤
JP2019126326A (ja) * 2018-01-26 2019-08-01 東ソー株式会社 多能性幹細胞の剥離方法
JP2020110140A (ja) * 2019-01-11 2020-07-27 東ソー株式会社 細胞培養方法
JP7647047B2 (ja) * 2020-01-21 2025-03-18 東ソー株式会社 低組織因子活性細胞、およびその製法
JP2021159049A (ja) * 2020-04-03 2021-10-11 ダイキン工業株式会社 接着性細胞を増殖させる方法、及びその方法のために用いられるマイクロキャリア

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6313822B2 (ja) 2010-08-31 2018-04-18 株式会社セルシード 温度応答性細胞培養用ビーズ及びその製造方法
JP6954047B2 (ja) 2017-11-22 2021-10-27 東ソー株式会社 ブロック共重合体コートビーズおよびその製造方法
JP2021106543A (ja) 2019-12-27 2021-07-29 ダイキン工業株式会社 温度応答性細胞培養用マイクロキャリア

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EP4600345A1 (en) 2025-08-13
JPWO2024075722A1 (https=) 2024-04-11
CN119998441A (zh) 2025-05-13

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