CN118000318A - Premixed feed for dairy cows in lactation period and preparation method and application thereof - Google Patents
Premixed feed for dairy cows in lactation period and preparation method and application thereof Download PDFInfo
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- CN118000318A CN118000318A CN202410246140.0A CN202410246140A CN118000318A CN 118000318 A CN118000318 A CN 118000318A CN 202410246140 A CN202410246140 A CN 202410246140A CN 118000318 A CN118000318 A CN 118000318A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
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- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention belongs to the technical field of animal feeds, and particularly relates to a premix feed for dairy cows in lactation period and a preparation method and application thereof. The feed composition of the invention comprises the following components: carrier, mineral, probiotics, allicin and aspergillus oryzae fermentation complex enzyme. The mineral substances are added into the premix feed composition for the cows for lactation, so that the somatic cell number in milk can be reduced, and the risk of diseases such as cow mastitis can be effectively reduced; the probiotics can regulate ecological conditions of rumen microorganisms and promote production performance of the rumen microorganisms; the allicin can promote the growth of cows, improve milk yield and milk fat percentage, and reduce feeding cost; the aspergillus oryzae fermentation complex enzyme can relieve heat stress of cows, has scientific and reasonable formula design and comprehensive and balanced nutrition, can provide balanced nutrition for cows in lactation period, is beneficial to improving the resistance and immune function of the cows in lactation period, improves the quality of milk and improves the economic benefit of farmers.
Description
Technical Field
The invention belongs to the technical field of animal feeds, and particularly relates to a premix feed for dairy cows in lactation period and a preparation method and application thereof.
Background
The feeding management of the lactating cows is directly related to the delivery condition, health condition and reproduction level of the cows, the milk yield and milk quality in the whole milk production period are also core work of the cattle farm, the economic benefit is directly influenced, and the continuous development of the cattle farm is ensured. The proportion of coarse and fine feed of the dairy cow feed is very important for the dairy cows, excessive fine feed can reduce the pH of rumen, the generation of propionic acid is the most increased, the generation of acetic acid is the least, the nutrient supply is increased, the milk yield is increased, but the efficiency of synthesizing milk fat is low, the milk fat rate is reduced, in addition, excessive feeding of the concentrate can cause diseases such as rumen poisoning, food intake laminitis, difficult pregnancy and the like. Research proves that the nutrition negative balance of the high-yield dairy cows needs to be reduced as much as possible, and the normal exertion of rumen functions is ensured, so that the health of the dairy cows can be maintained, and the stable yield and the high yield are ensured.
The Chinese patent CN103229898A discloses a dairy cow feed and a preparation method thereof, wherein the dairy cow feed comprises the following raw materials in parts by weight: 40-50 parts of rape straw, 20-30 parts of milk vetch, 15-25 parts of sweet potato leaves, 15-20 parts of cotton seed hulls, 10-15 parts of peanut vines, 15-20 parts of flour, 10-15 parts of corn grits, 1-2 parts of leavening agent, 40-45 parts of oat flour, 20-25 parts of high Liang Kang parts of fried rice flour, 3-5 parts of soft candy additives, 4-5 parts of rice bran, 5-8 parts of clay sepiolite, 3-4 parts of palm oil, 2-3 parts of sodium alginate, 1-2 parts of calcium carbonate, 1-1.5 parts of sodium hydrophosphate and 0.2-0.4 part of salt.
The Chinese patent CN104585531A discloses a milk cow feed for promoting the growth and lactation of milk cows in lactation period, which comprises the following raw materials in parts by weight: 30-33 parts of corn flour, 30-33 parts of sorghum flour, 14-17 parts of prefabricated active materials, 12-15 parts of konjak, 8-12 parts of wheat flour, 7-10 parts of barley bran, 5-8 parts of straw powder, 3-5 parts of dry beet pulp, 5-8 parts of carrot, 6-9 parts of fishbone powder, 6-9 parts of whey powder, 3.5-5 parts of soybean oil, 2-4 parts of salt, 2-2.5 parts of brown sugar, 1-2 parts of calcium hydrophosphate, 0.1-0.3 part of lysine, 0.2-0.5 part of choline, 0.1-0.2 part of cod liver oil and 0.06-0.1 part of magnesium sulfate.
However, the dairy cow feed formula has certain limitations such as purpose, adaptation and the like, and can not completely meet the growth needs of dairy cows. Therefore, in order to solve a series of problems such as milk cow feed intake reduction, energy negative balance and immune function reduction, the field is urgent to develop a feed which has reasonable formula and can improve the milk cow resistance and immune function, and has important significance for improving milk quality and economic benefit of farmers.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a pre-mixed feed for dairy cows in lactation period and a preparation method and application thereof. The invention reduces the influence of physiological factors on organisms to the greatest extent, adds nutrient components such as mineral elements, water-soluble allicin, probiotics and the like, supplements balanced nutrition for cows, assists digestion and absorption of cows, recovers bodies, promotes growth and development of fetuses, improves resistance and immune functions of cows, maintains the health condition of cows, and lays a foundation for perinatal period and even lactation period.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a feed composition comprising the following components: carrier, probiotics, minerals, allicin and aspergillus oryzae fermentation complex enzyme.
Preferably, the carrier is rice chaff powder, and the mineral is one or more selected from calcium carbonate, calcium hydrophosphate, magnesium sulfate, sodium chloride, ferrous sulfate heptahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, manganese sulfate pentahydrate, selenium-enriched yeast and cobalt chloride hexahydrate.
Preferably, the probiotics are selected from one or more of bacillus paralicheniformis, bacillus amyloliquefaciens and bacillus subtilis.
More preferably, the probiotics are a mixture of bacillus paratlicheniformis and bacillus amyloliquefaciens in a weight ratio of 1-1.5:0.5-2.
Preferably, the composition further comprises lysine, methionine, saccharomyces cerevisiae, antioxidants and chromium propionate.
Preferably, the antioxidant is ethoxyline quinolin, dibutyl hydroxy toluene or propyl gallate.
Preferably, the composition comprises the following components in percentage by weight: 1-5% of carrier, 0.01-0.05% of antioxidant, 0.01-0.05% of probiotics, 0.1-0.5% of allicin, 70-80% of mineral substances, 8-12% of lysine, 1-5% of methionine, 1-5% of saccharomyces cerevisiae, 1-5% of aspergillus oryzae fermentation complex enzyme and 0.01-0.05% of chromium propionate.
More preferably, the composition comprises the following components in percentage by weight: 4-5% of carrier, 0.01-0.02% of antioxidant, 0.03-0.05% of probiotics, 0.1-0.2% of allicin, 70-75% of mineral substances, 9-11% of lysine, 4-5% of methionine, 2-4% of saccharomyces cerevisiae, 3-5% of aspergillus oryzae fermentation complex enzyme and 0.04-0.05% of chromium propionate.
The invention also provides a preparation method of the feed composition, which comprises the following steps: weighing mineral, carrier, antioxidant, probiotics, garlicin, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate, mixing for 20-40min, packaging, and inspecting.
The invention also provides a premix feed for the dairy cows in the lactation period, which comprises the feed composition.
The invention also provides application of the feed composition or the premix feed for the dairy cows in the lactation period in the aspect of raising the dairy cows.
Compared with the prior art, the invention has the following beneficial effects:
(1) The premix feed composition for the lactating cows has more scientific and reasonable formula design and balanced nutrition, can provide balanced nutrition for the cows in the lactation period, is beneficial to improving the resistance and immune function of the lactating cows, is beneficial to improving the quality of milk and improves the economic benefit of farmers.
(2) The mineral substances are added into the feed, so that the feed is safe and easy to absorb, can reduce the number of somatic cells in milk, and effectively reduces the risks of diseases such as cow mastitis; the garlicin is a plant broad-spectrum antibiotic, has the characteristics of low residue, low cost, strong food calling property, strong flavoring property and the like, has no drug resistance, can promote the growth of dairy cows, improves milk yield and milk fat percentage, prevents and treats related diseases, and reduces feeding cost; the probiotics can regulate ecological conditions of rumen microorganisms, promote production performance of the rumen microorganisms, improve feed utilization rate and improve health of ruminants; the coated lysine and methionine can increase milk yield of the dairy cows, improve metabolic disturbance and improve reproductive performance; saccharomyces cerevisiae can enhance the production performance of cows; aspergillus oryzae fermentation complex enzyme and chromium propionate relieve heat stress of cows, and improve the problem of milk consumption and milk yield reduction caused by heat stress.
(3) Animal experiments prove that the mixed feed composition for the dairy cows in the lactation period has obvious improvement effect, and the premixed feed for the dairy cows in the lactation period is used for feeding the dairy cows, is beneficial to rapid absorption of various special energy substances, can increase milk yield and improve milk quality, and can effectively increase the content of milk fat, milk protein, lactose and milk dry matter in milk.
Detailed Description
The following non-limiting examples will enable those of ordinary skill in the art to more fully understand the invention and are not intended to limit the invention in any way. The following is merely exemplary of the scope of the invention as claimed and many variations and modifications of the invention will be apparent to those skilled in the art in light of the disclosure, which are intended to be within the scope of the invention as claimed. Unless otherwise indicated, materials or reagents provided in the examples are all commonly commercially available products, and are exemplified below.
The Bacillus licheniformis is a Bacillus licheniformis SN-6 strain, and the Bacillus amyloliquefaciens is a Bacillus amyloliquefaciens SN-C strain, which are prepared by referring to the example 1 of the invention patent CN 111996139A; aspergillus niger Aspergillus niger, CICC 40048, purchased from China center for type culture Collection; allicin, 98% pure, purchased from ataxia luya biotechnology limited; lysine is L-lysine hydrochloride with purity of 99%, and is purchased from Wohan Baixing biotechnology Co., ltd; methionine is DL-methionine with purity of 99% and is purchased from Ying Chuang industrial group; saccharomyces cerevisiae is a culture of Saccharomyces cerevisiae available from Oriental veterinary medicine Inc., cangzhou; aspergillus oryzae fermenting complex enzyme with purity of 95% purchased from Shandong and Zhong kang Source biotechnology Co., ltd; chromium propionate, 98% pure, was purchased from cistans biotechnology limited.
Example 1
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 0.4g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1:1), 1.0g of garlicin, 740.2g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged products are qualified through inspection.
Example 2
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 50g of rice chaff powder, 0.5g of propyl gallate, 0.5g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1:1.5), 5.0g of allicin, 693.5g of magnesium sulfate, 120g of lysine, 50g of methionine, 30g of saccharomyces cerevisiae, 50g of aspergillus oryzae fermentation complex enzyme and 0.5g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, propyl gallate, probiotics, allicin, magnesium sulfate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, propyl gallate, probiotics, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank together, fully mixing for 20min, subpackaging after mixing, and warehousing after the packaged products are qualified after inspection.
Example 3
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 20g of rice chaff powder, 0.1g of ethoxyquin, 0.1g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1.5:1), 1.0g of allicin, 800g of zinc sulfate heptahydrate, 80g of lysine, 28.7g of methionine, 50g of saccharomyces cerevisiae, 20g of aspergillus oryzae fermentation complex enzyme and 0.1g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, ethoxyquin, probiotics, allicin, zinc sulfate heptahydrate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, ethoxyquin, probiotics, allicin, zinc sulfate heptahydrate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 40min, subpackaging after mixing, and warehousing after the packaged products are qualified after inspection.
Example 4
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 40g of rice chaff powder, 0.3g of ethoxyquin, 0.3g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1:2), 3.0g of garlicin, 729g of cobalt chloride hexahydrate, 112g of lysine, 45g of methionine, 30g of saccharomyces cerevisiae, 40g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, ethoxyquin, probiotics, garlicin, cobalt chloride hexahydrate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, ethoxyquin, probiotics, garlicin, cobalt chloride hexahydrate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 40min, subpackaging after mixing, and warehousing after the packaged products are qualified after inspection.
Comparative example 1
The only difference compared to example 1 is the substitution of allicin for the antibacterial peptide.
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 0.4g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1:1), 1.0g of antibacterial peptide, 740.2g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, probiotics, antibacterial peptide, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, the dibutyl hydroxy toluene, the probiotics, the antibacterial peptide, the calcium carbonate, the lysine, the methionine, the saccharomyces cerevisiae, the aspergillus oryzae fermentation complex enzyme and the chromium propionate into a mixing tank together, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged products are qualified through inspection.
Comparative example 2
The only difference compared to example 1 is that no probiotics are contained.
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 1.0g of allicin, 740.6g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, allicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting into a mixing tank, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged product is qualified after inspection.
Comparative example 3
The only difference compared to example 1 is the substitution of the Bacillus paratlicheniformis SN-6 strain and the Bacillus amyloliquefaciens SN-C strain with Aspergillus niger.
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 0.4g of probiotics (aspergillus niger), 1.0g of garlicin, 740.2g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged products are qualified through inspection.
Comparative example 4
The difference compared to example 1 is only that the weight ratio of Bacillus paratlicheniformis SN-6 to Bacillus amyloliquefaciens SN-C is 1:3.
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 0.4g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 1:3), 1.0g of garlicin, 740.2g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged products are qualified through inspection.
Comparative example 5
The difference compared to example 1 is only that the weight ratio of Bacillus paratlicheniformis SN-6 to Bacillus amyloliquefaciens SN-C is 3:1.
The premixed feed for the lactation period of the dairy cows is prepared from the following components in 1000g by weight: 48.3g of rice chaff powder, 0.1g of dibutyl hydroxy toluene, 0.4g of probiotics (the weight ratio of the auxiliary bacillus licheniformis SN-6 strain to the starch bacillus SN-C strain is 3:1), 1.0g of garlicin, 740.2g of calcium carbonate, 101.0g of lysine, 40.4g of methionine, 28.4g of saccharomyces cerevisiae, 39.8g of aspergillus oryzae fermentation complex enzyme and 0.4g of chromium propionate.
A preparation method of a premix feed for dairy cows in lactation period comprises the following steps:
Weighing rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate according to the corresponding weight, putting the rice chaff powder, dibutyl hydroxy toluene, probiotics, garlicin, calcium carbonate, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate into a mixing tank, fully mixing for 30min, subpackaging after mixing, and warehousing after the packaged products are qualified through inspection.
Effect example
1. Experimental materials and methods
(1) Time and place of experiment
Cow tests were completed in 2022 at the cattle farm of the livestock technology development limited company in martial arts Jin Xu in Hubei province.
(2) Laboratory animals and groups
Test 110 Holstein cows with similar ages, weights, fetuses and lactation days are selected, 10 healthy cows with the number of somatic cells less than 20 ten thousand/mL are set as healthy groups, 100 clinical mastitis cows with the number of somatic cells greater than 50 ten thousand/mL are divided into 10 groups according to the principle of random distribution, and the mastitis groups and experimental groups (the pre-mixed feed for the lactation period of the cows prepared in examples 1-4 and comparative examples 1-5). The healthy group cows and the mastitis group cows are fed with basic daily ration (the basic daily ration is prepared by referring to NY/T34-2004 cow feeding standard), the experimental group cows are added with the pre-mixed feed for the lactation period of the cows prepared in examples 1-4 and comparative examples 1-5 according to the proportion of 0.05% on the basis of feeding the basic daily ration every day, and the test period is 40 days in total.
(3) Sample collection and preservation
Cow blood is collected by adopting a tail root vein blood collection mode when the cows do not ingest in the test period on the 0 th day and the 40 th morning, 10mL of blood is collected by each cow, and the blood is slowly pumped out by a syringe and placed in a 15mL centrifuge tube in the blood collection process, so that hemolysis is avoided. Centrifuging the centrifugal tube for 15min at a speed of 3000r/min, sucking out upper serum, placing into the centrifugal tube, placing the obtained serum into 2 tubes on average after centrifuging each cow, and storing all serum samples in a refrigerator at-80 ℃. At day 0, 20 and 40, milk samples were collected with 50mL centrifuge tubes at early and late milking, following the following: mixing at a volume ratio of late=4:6, adding 0.05g of potassium dichromate powder into 50mL of milk, shaking uniformly, and storing in a refrigerator at 4 ℃ for testing.
2. Test index and detection method
2.1 Determination of milk yield and milk quality of cows
All test cows were milked twice daily (05:30 in the morning and 17:30 in the afternoon), the cows automatically recorded the milk yield when using the fishbone milking platform, and the milking workers recorded the milk yield of all cows during milking, and the sum of the milk yields of the two times in the morning and evening was the milk yield of each cow on the day. The collected milk samples were sent to the Hubei cow productivity measuring center (Hubei, wuhan) within 48 hours, and milk proteins, milk fat, lactose, non-milk fat solids and total solids were detected in the milk using a milk component analyzer (FT 600, foss Electric, denmark), and the milk samples were subjected to somatic counting at the same time as the measurement of milk was performed conventionally using a somatic counting machine (Somacout).
2.2 Serum Biochemical index
The detection of prolactin, luteinizing hormone, immunoglobulin A and progesterone in blood all used the corresponding bovine ELISA kit from enzyme-immune biosystems.
2.3 Cow antioxidant index
Superoxide dismutase (Superoxide dismutase, SOD), glutathione peroxidase (Glutathione peroxidase, GSH-Px), total antioxidant capacity (Total cholesterol, T-AOC) and malondialdehyde (Malondialdehyde, MDA) were detected by using an enzyme-labeled detector (USCNK, SMR 60047) according to the method provided by Nanjing's build company kit.
3. Experimental results and analysis
3.1 Effect of premix feed on the production Performance of mastitis cows
As can be seen from Table 1, the daily milk yield of the healthy and experimental groups of cows is significantly higher than that of the Yu Ruxian inflammatory group, wherein the daily milk yield of the pre-mixed feed prepared in examples 1-4 in the experimental group is higher than that of the comparative examples 1-5, and the pre-mixed feed provided by the invention can improve the daily milk yield of the mastitis cows.
As can be seen from Table 2, the healthy group had significantly higher milk quality in Yu Ruxian inflammatory groups of cows, and the experimental groups showed significantly higher milk fat, milk protein and lactose levels than the mastitis groups of examples 1-4.
TABLE 1 Effect of the premix feed of the invention on daily milk yield and milk somatic cell count in mastitis cows
Note that: the above table is marked with different letters to indicate that the difference is significant.
TABLE 2 Effect of the premix feed of the invention on milk quality
Note that: the above table is marked with different letters to indicate that the difference is significant.
3.2 Influence of premixed feed on cow serum biochemical index by mastitis
From tables 3-4, it can be seen that from day 0 to day 40, the healthy and experimental groups of cows had significantly higher prolactin, progesterone and immunoglobulin a than the Yu Ruxian inflammatory group of cows, wherein the premix feed prepared in examples 1-2 in the experimental group had higher luteinizing hormone, prolactin, progesterone and immunoglobulin a for the cows than comparative examples 1, 3 and 4.
TABLE 3 Effect of the premix feed of the invention on luteinizing hormone and prolactin levels in serum of mastitis cows
TABLE 4 Effect of the premix feed of the invention on the progesterone and immunoglobulin A levels in the serum of mastitis cows
Note that: the above table is marked with different letters to indicate that the difference is significant.
3.3 Effect of premix feed on antioxidant function in serum of mastitis cows
From tables 5 to 6, it is understood that the superoxide dismutase, catalase and malondialdehyde contents of the cows in the mastitis group and the experimental group were not different from those of the cows in the healthy group from day 0 to day 40, and the total antioxidant capacity of the cows in the mastitis group and the experimental group was lower than that of the cows in the healthy group at day 0. On day 40, the total antioxidant capacity of the dairy cows of the experimental group is obviously higher than that of the group Yu Ruxian, wherein the pre-mixed feed prepared in the experimental group examples 1, 3 and 4 has higher superoxide dismutase, catalase content and total antioxidant capacity than those of the experimental group comparative examples 1, 3 and 5 in serum of the dairy cows with mastitis.
TABLE 5 Effect of the premix feed of the invention on superoxide dismutase, glutathione peroxidase and catalase levels in serum of mastitis cows
TABLE 6 influence of the premix feed of the invention on the total antioxidant capacity and propylene glycol content in serum of mastitis cows
Note that: the above table is marked with different letters to indicate that the difference is significant.
4. Conclusion of the experiment
After the dairy cows suffer from mastitis, the milk yield, the milk protein rate, the milk fat rate, the lactose rate and the non-milk fat solid rate are reduced to different degrees, the immunity and the antioxidation capability of the body are inhibited, the total antioxidation capability in serum is obviously reduced, and after the pre-mixed feed provided by the invention is fed to the mastitis dairy cows, the production performance, the immunity and the antioxidation level are obviously improved, and the mastitis of the dairy cows is improved.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. A feed composition comprising the following components: carrier, probiotics, minerals, allicin and aspergillus oryzae fermentation complex enzyme.
2. The feed composition of claim 1, wherein the carrier is rice chaff powder and the mineral is selected from one or more of calcium carbonate, calcium hydrogen phosphate, magnesium sulfate, sodium chloride, ferrous sulfate heptahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, manganese sulfate pentahydrate, selenium-enriched yeast, and cobalt chloride hexahydrate.
3. The feed composition of claim 1, wherein the probiotic is selected from one or more of the group consisting of bacillus paralicheniformis, bacillus amyloliquefaciens and bacillus subtilis.
4. A feed composition according to claim 3, wherein the probiotic is a mixture of bacillus paratlicheniformis and bacillus amyloliquefaciens in a weight ratio of 1-1.5:0.5-2.
5. The feed composition of claim 1, further comprising lysine, methionine, saccharomyces cerevisiae, an antioxidant and chromium propionate, the antioxidant being ethoxyquin, dibutylhydroxytoluene, or propyl gallate.
6. The feed composition of claim 5, comprising the following components in weight percent: 1-5% of carrier, 0.01-0.05% of antioxidant, 0.01-0.05% of probiotics, 0.1-0.5% of allicin, 70-80% of mineral substances, 8-12% of lysine, 1-5% of methionine, 1-5% of saccharomyces cerevisiae, 1-5% of aspergillus oryzae fermentation complex enzyme and 0.01-0.05% of chromium propionate.
7. The feed composition of claim 6, comprising the following components in weight percent: 4-5% of carrier, 0.01-0.02% of antioxidant, 0.03-0.05% of probiotics, 0.1-0.2% of allicin, 70-75% of mineral substances, 9-11% of lysine, 4-5% of methionine, 2-4% of saccharomyces cerevisiae, 3-5% of aspergillus oryzae fermentation complex enzyme and 0.04-0.05% of chromium propionate.
8. A process for the preparation of a feed composition as claimed in any one of claims 5 to 7, comprising the steps of: weighing mineral, carrier, antioxidant, probiotics, garlicin, lysine, methionine, saccharomyces cerevisiae, aspergillus oryzae fermentation complex enzyme and chromium propionate, mixing for 20-40min, packaging, and inspecting.
9. A premix feed for dairy cows during lactation, comprising a feed composition as claimed in any of claims 1 to 7.
10. Use of a premix feed according to claim 9 or a feed composition prepared by a method according to claim 8 for feeding cows.
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