CN117902934A - Preparation and application methods of high-nutrient high-activity chemical fertilizer biological compound fertilizer for rice - Google Patents
Preparation and application methods of high-nutrient high-activity chemical fertilizer biological compound fertilizer for rice Download PDFInfo
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 170
- 150000001875 compounds Chemical class 0.000 title claims abstract description 94
- 239000000126 substance Substances 0.000 title claims abstract description 45
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 44
- 235000009566 rice Nutrition 0.000 title claims abstract description 44
- 230000000694 effects Effects 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 240000007594 Oryza sativa Species 0.000 title claims 3
- 230000000813 microbial effect Effects 0.000 claims abstract description 99
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 43
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 39
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 39
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 27
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 23
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 23
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 17
- 229940072056 alginate Drugs 0.000 claims abstract description 11
- -1 alginate oligosaccharides Chemical class 0.000 claims abstract description 11
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 11
- 229920000615 alginic acid Polymers 0.000 claims abstract description 11
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 11
- OTPDWCMLUKMQNO-UHFFFAOYSA-N 1,2,3,4-tetrahydropyrimidine Chemical compound C1NCC=CN1 OTPDWCMLUKMQNO-UHFFFAOYSA-N 0.000 claims abstract description 9
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 9
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims abstract description 9
- 239000000618 nitrogen fertilizer Substances 0.000 claims abstract description 9
- 239000002686 phosphate fertilizer Substances 0.000 claims abstract description 9
- 229940072033 potash Drugs 0.000 claims abstract description 9
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims abstract description 9
- 235000015320 potassium carbonate Nutrition 0.000 claims abstract description 9
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 7
- 239000008158 vegetable oil Substances 0.000 claims abstract description 7
- 239000003223 protective agent Substances 0.000 claims abstract description 4
- 241000194107 Bacillus megaterium Species 0.000 claims description 16
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000004115 Sodium Silicate Substances 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 8
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 239000006012 monoammonium phosphate Substances 0.000 claims description 8
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 8
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 229960001763 zinc sulfate Drugs 0.000 claims description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 8
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 6
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 6
- 235000011151 potassium sulphates Nutrition 0.000 claims description 6
- 235000012424 soybean oil Nutrition 0.000 claims description 5
- 239000003549 soybean oil Substances 0.000 claims description 5
- 235000019438 castor oil Nutrition 0.000 claims description 4
- 239000004359 castor oil Substances 0.000 claims description 4
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- XZMCDFZZKTWFGF-UHFFFAOYSA-N Cyanamide Chemical compound NC#N XZMCDFZZKTWFGF-UHFFFAOYSA-N 0.000 claims description 2
- 239000001166 ammonium sulphate Substances 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- MYFXBBAEXORJNB-UHFFFAOYSA-N calcium cyanamide Chemical compound [Ca+2].[N-]=C=[N-] MYFXBBAEXORJNB-UHFFFAOYSA-N 0.000 claims description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 2
- 235000013877 carbamide Nutrition 0.000 claims description 2
- 239000002367 phosphate rock Substances 0.000 claims description 2
- 239000002426 superphosphate Substances 0.000 claims description 2
- 241000209094 Oryza Species 0.000 abstract description 41
- 239000002689 soil Substances 0.000 abstract description 27
- 241000196324 Embryophyta Species 0.000 abstract description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 9
- 238000011161 development Methods 0.000 abstract description 6
- 208000035240 Disease Resistance Diseases 0.000 abstract description 5
- 230000006872 improvement Effects 0.000 abstract description 5
- 230000006806 disease prevention Effects 0.000 abstract description 3
- 230000007102 metabolic function Effects 0.000 abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 29
- 230000000052 comparative effect Effects 0.000 description 27
- 238000011282 treatment Methods 0.000 description 23
- 229910052757 nitrogen Inorganic materials 0.000 description 16
- 229910052698 phosphorus Inorganic materials 0.000 description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 13
- 235000021049 nutrient content Nutrition 0.000 description 13
- 239000011574 phosphorus Substances 0.000 description 13
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 12
- 239000011591 potassium Substances 0.000 description 12
- 229910052700 potassium Inorganic materials 0.000 description 12
- 230000008569 process Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
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- 238000011835 investigation Methods 0.000 description 2
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- 240000001987 Pyrus communis Species 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
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- 230000014759 maintenance of location Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
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Abstract
The invention relates to a preparation and application method of a high-nutrient high-activity chemical fertilizer biological compound fertilizer for paddy rice, which belongs to the technical field of fertilizers, and the compound fertilizer provided by the invention comprises the following components: inorganic nitrogen fertilizer, inorganic phosphate fertilizer, inorganic potash fertilizer, microbial agent and microbial protective agent; the microbial agent comprises two or more than two of bacillus subtilis, bacillus mucilaginosus and trichoderma harzianum; the microbial protectant comprises brown alginate oligosaccharides, vegetable oil, tetrahydropyrimidine and citric acid; the special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice maintains the activity of microorganisms, increases the supply of available nutrients, enhances the metabolic function and disease resistance and disease prevention capability of plants, promotes the growth and development of rice, realizes the improvement of yield and quality, and can effectively improve soil and prevent soil hardening.
Description
Technical Field
The invention relates to a preparation and application method of a high-nutrient high-activity chemical fertilizer biological compound fertilizer for rice, belonging to the technical field of fertilizers.
Background
Chemical fertilizer is one of important substance inputs in agricultural production, and according to the statistics of grain and agricultural organization (FAO) of united nations, the contribution of the chemical fertilizer to the yield increase of crops is more than half. The application of chemical fertilizers plays a significant role in ensuring the effective supply of agricultural products. Application of fertilizers has been one of the most effective agricultural product yield increasing measures in the past, present and future. However, chemical fertilizers are easy to decompose and run off, cannot be absorbed and utilized by crops in time, have low utilization rate, and with the importance of ecological protection and green agriculture, the problem of farmland pollution caused by chemical fertilizer use is more and more concerned, microorganisms have remarkable effects on soil improvement, microbial agents and organic matters are combined to prepare biological organic fertilizers which are widely applied in production, and the biological organic fertilizers participate in fixation of nitrogen in soil, conversion of substances and energy and decomposition and release of nutrients in organic matters through vital movements of the microorganisms in the soil, so that the fertility of the soil is improved, and the effect of improving the soil is achieved. But the inorganic nutrient content in the bio-organic fertilizer is low, and the requirements of crops on nitrogen, phosphorus and potassium are difficult to meet. If the microbial agent can be combined with high-concentration fertilizer and the stable microbial activity is maintained, the requirements of crops on inorganic nutrients can be met, and the improvement of microorganisms on soil can be realized. Therefore, development of a biological compound fertilizer with high microbial activity and stability, long retention time and high chemical nutrient content is needed.
The existing fertilizer prepared by compounding the functional microbial agent and the nutrient substances has low nitrogen, phosphorus and potassium nutrient content, the total nutrient content of nitrogen (N), phosphorus (P 2O5) and potassium (K 2 O) is lower than 25%, the salt index of the fertilizer can be necessarily increased to improve the nutrient content of the fertilizer, and if the microbial agent is protected by no related measures, the activity of the microbial agent can be reduced or even completely inactivated. Therefore, the uniform mixing of the fertilizer with high salt index and the living microorganism to prepare the compound fertilizer containing high-activity microorganism and high-nitrogen, phosphorus and potassium nutrients is a technical problem in the industry. The long-term application of chemical fertilizer in rice production improves the yield, but causes the problems of soil hardening, quality degradation, water pollution and the like, the nitrogen and phosphorus nutrients lost by the field runoff of the rice field become the main sources of agricultural non-point source pollution, and the application of microbial fertilizer can improve the soil environment, improve the fertilizer utilization rate and reduce the environmental pollution, but can increase the fertilization cost and the labor investment, so the development of the high-activity high-concentration chemical fertilizer biological compound fertilizer suitable for rice production is needed to realize the investment of high-activity microbial inoculant and high nutrient, and avoid the increase of the fertilization cost and the labor investment.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation and application method of a high-nutrient high-activity chemical fertilizer biological compound fertilizer for rice.
Solves the problems of complex process, high cost, unstable effective viable count and poor application effect of the microbial agent added in the existing fertilizer production.
The nutrient content of nitrogen, phosphorus and potassium in the invention refers to the content of N, P 2O5 and K 2 O.
The technical scheme of the invention is as follows:
A special high-activity high-concentration chemical fertilizer biological compound fertilizer for paddy rice comprises the following components: inorganic nitrogen fertilizer, inorganic phosphate fertilizer, inorganic potash fertilizer, microbial agent and microbial protective agent;
the microbial agent comprises the following components: one or more than two of bacillus subtilis, bacillus mucilaginosus and trichoderma harzianum;
the microbial protectant comprises: brown alginate oligosaccharides, vegetable oil, tetrahydropyrimidine and citric acid;
the inorganic nitrogen fertilizer comprises: ammonium chloride, ammonium sulphate, urea and/or lime nitrogen [ cyanamide based calcium (CaCN 2) ];
The inorganic phosphate fertilizer comprises: monoammonium phosphate, superphosphate and/or ground phosphate rock;
the inorganic potash fertilizer comprises: potassium chloride and/or potassium sulfate.
The brown alginate oligosaccharides can be prepared according to the existing method, and can be referred to patent document CN 112342255A or CN 112941089A, and are also commercially available.
The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice has the nutrient content of nitrogen, phosphorus and potassium of more than or equal to 40 percent.
According to the preferred microbial agent of the invention, the bacillus subtilis is one or more of bacillus subtilis ACCC60364, bacillus subtilis ACCC19743 or bacillus subtilis CGMCC No. 29499;
The bacillus mucilaginosus is bacillus mucilaginosus CGMCC No.17376 and/or bacillus mucilaginosus ACCC10095;
The Trichoderma harzianum is Trichoderma harzianum ACCC33109 and/or Trichoderma harzianum ACCC32072.
According to the invention, preferably, the microbial agent further comprises bacillus megatherium.
Further preferably, the bacillus megaterium is bacillus megaterium CGMCC No.29498.
According to the invention, the components of the compound fertilizer also comprise sodium silicate and zinc sulfate.
According to the invention, the compound fertilizer preferably comprises the following components in parts by weight:
15-40 parts of inorganic nitrogen fertilizer, 20-50 parts of inorganic phosphate fertilizer and 10-20 parts of inorganic potash fertilizer;
The microbial agent comprises the following components: the ratio of the number of the viable bacteria of the bacillus subtilis, the bacillus mucilaginosus and the trichoderma harzianum is (35-55): 1 (1-3);
the viable count of the microorganism bacteria in the compound fertilizer is more than 2 hundred million cfu/g;
The mass ratio of the microbial protectant to the microbial inoculant in the compound fertilizer is more than or equal to 1;
the microbial protectant comprises the following components in parts by weight: 65-85 parts of brown alginate oligosaccharides, 5-10 parts of vegetable oil, 5-8 parts of tetrahydropyrimidine and 6-10 parts of citric acid.
Further preferably, the microbial agent comprises: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2.
Further preferably, the mass ratio of the microbial protectant to the microbial inoculum in the compound fertilizer is (1-100): 1.
Further preferably, the vegetable oil comprises: soybean oil and/or castor oil.
Further preferably, the effective viable count of the microbial agent is more than 1000 hundred million cfu/gram.
Further preferably, the inorganic nitrogen fertilizer is urea, ammonium sulfate and/or ammonium chloride; the inorganic phosphate fertilizer is monoammonium phosphate; the inorganic potash fertilizer is potassium chloride and/or potassium sulfate.
Further preferably, the compound fertilizer further comprises, in parts by weight: 1-2 parts of sodium silicate and 1-2 parts of zinc sulfate.
The preparation method of the special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice comprises the following steps:
And uniformly mixing the microbial agent and the microbial protectant, and uniformly mixing with other components to prepare the compound fertilizer.
Further preferably, the microbial agent and the microbial protectant are uniformly mixed, then uniformly mixed with other components, and granulated to obtain the compound fertilizer.
Advantageous effects
1. After the compound fertilizer provided by the invention is applied, the microbial community of the rhizosphere of rice can be improved, the disease resistance and stress resistance of the rice are enhanced, the yield is increased, and the quality is improved.
2. The compound fertilizer provided by the invention is effectively combined with the microbial agent, and the prepared fertilizer biological compound fertilizer contains high-concentration active microorganisms, so that the fertilizer can still have a certain amount of microbial activity within a period of time after being applied to soil.
3. The fertilizer has low preparation and production cost and is easy to popularize and use.
Detailed Description
Embodiments of the present invention are described in further detail below with reference to examples.
The following examples are illustrative of the invention but are not intended to limit the scope of the invention.
All not described in detail in the examples are prior art.
Brown alginate oligosaccharides, soybean oil, castor oil, tetrahydropyrimidine and citric acid are all commercial products and can also be prepared by a conventional method.
In the present invention, the brown alginate oligosaccharides can be prepared according to the existing method, and refer to patent document CN 112342255A or CN 112941089A, and are also commercially available.
The microbial agents described in the examples were all manufactured by Shandong Li Source bioengineering Co., ltd, and commercially available products were also purchased.
Bacillus subtilis (Bacillus subtilis), deposited in China general microbiological culture Collection center, address: the preservation number of the Qingyang area beichen Xili No. 1 and 3 of Beijing is CGMCC No.29499.
Bacillus megaterium (Bacillus megaterium), deposited in China general microbiological culture Collection center, address: the preservation number of the Qingyang area beichen Xili No.1 and 3 of Beijing is CGMCC No.29498.
Example 1
The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice comprises the following components in parts by weight:
21 parts of urea, 15 parts of ammonium sulfate, 50 parts of monoammonium phosphate, 10 parts of potassium chloride, 1 part of sodium silicate and 1 part of zinc sulfate;
the microbial agent comprises: bacillus subtilis ACCC60364, bacillus mucilaginosus CGMCC No.17376, trichoderma harzianum ACCC33109;
The microbial protectant comprises the following components in parts by weight: 75 parts of brown alginate oligosaccharides, 8 parts of soybean oil, 8 parts of tetrahydropyrimidine and 9 parts of citric acid;
The microbial agent comprises the following components: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2;
The effective viable count of the microbial agent is 1000 hundred million cfu/g.
In the compound fertilizer, the mass ratio of the microbial protectant to the microbial inoculant is 2:1.
The microbial viable count in the compound fertilizer is 2 hundred million cfu/g, and the nitrogen, phosphorus and potassium nutrient content is more than 40%.
The preparation method of the compound fertilizer comprises the following steps:
And uniformly mixing the microbial agent and the microbial protectant, and uniformly mixing with other components to prepare the compound fertilizer. The viable count of the microorganism in the compound fertilizer is 2 hundred million cfu/g.
Example 2
The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice comprises the following components in parts by weight:
20 parts of urea, 14 parts of ammonium chloride, 50 parts of monoammonium phosphate, 12 parts of potassium sulfate, 1 part of sodium silicate and 1 part of zinc sulfate;
the microbial agent comprises: bacillus subtilis ACCC60364, bacillus mucilaginosus CGMCC No.17376, trichoderma harzianum ACCC33109;
the microbial protectant comprises the following components in parts by weight: 78 parts of brown alginate oligosaccharides, 6 parts of castor oil, 8 parts of tetrahydropyrimidine and 8 parts of citric acid;
The microbial agent comprises the following components: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2;
The effective viable count of the microbial agent is 1500 hundred million cfu/g.
In the compound fertilizer, the mass ratio of the microbial protectant to the microbial inoculant is 3:1.
The viable count of the microorganism in the compound fertilizer is 2.5 hundred million cfu/g.
The preparation method of the compound fertilizer is the same as that of the example 1, wherein the microbial viable count in the compound fertilizer is 2.5 hundred million cfu/g, and the nutrient content of nitrogen, phosphorus and potassium is more than 40%.
Example 3
The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice comprises the following components in parts by weight:
21 parts of urea, 15 parts of ammonium sulfate, 50 parts of monoammonium phosphate, 10 parts of potassium chloride, 1 part of sodium silicate and 1 part of zinc sulfate;
The microbial agent comprises: bacillus subtilis ACCC19743, bacillus mucilaginosus ACCC10095, trichoderma harzianum ACCC32072;
The microbial protectant comprises the following components in parts by weight: 75 parts of brown alginate oligosaccharides, 8 parts of soybean oil, 8 parts of tetrahydropyrimidine and 9 parts of citric acid;
The microbial agent comprises the following components: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2;
The effective viable count of the microbial agent is 1000 hundred million cfu/g.
In the compound fertilizer, the mass ratio of the microbial protectant to the microbial inoculant is 2:1.
The viable count of the microorganism in the compound fertilizer is 2 hundred million cfu/g.
The preparation method of the compound fertilizer is the same as that of the example 1, wherein the microbial viable count in the compound fertilizer is 2 hundred million cfu/g, and the nutrient content of nitrogen, phosphorus and potassium is more than 40%.
Example 4
The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice comprises the following components in parts by weight:
20 parts of urea, 14 parts of ammonium chloride, 50 parts of monoammonium phosphate, 12 parts of potassium sulfate, 1 part of sodium silicate and 1 part of zinc sulfate;
The microbial agent comprises: bacillus subtilis ACCC19743, bacillus mucilaginosus ACCC10095, trichoderma harzianum ACCC32072;
The microbial agent comprises the following components: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2;
The effective viable count of the microbial agent is 1500 hundred million cfu/g.
In the compound fertilizer, the mass ratio of the microbial protectant to the microbial inoculant is 3:1.
The viable count of the microorganism in the compound fertilizer is 2.5 hundred million cfu/g.
The preparation method of the compound fertilizer is the same as that of the example 1, wherein the microbial viable count in the compound fertilizer is 2.5 hundred million cfu/g, and the nutrient content of nitrogen, phosphorus and potassium is more than 40%.
Comparative example 1
Biological compound fertilizer for chemical fertilizer
The difference from example 1 is that no microbial protectant is added, all other things being equal.
Comparative example 2
Biological compound fertilizer for chemical fertilizer
The difference from example 2 is that no microbial protectant is added, all other things being equal.
Comparative example 3
Biological compound fertilizer for chemical fertilizer
The difference from example 3 is that no microbial protectant is added, all other things being equal.
Comparative example 4
Biological compound fertilizer for chemical fertilizer
The difference from example 4 is that no microbial protectant is added, all other things being equal.
Example 5
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 1 is that the Bacillus subtilis ACCC60364 is replaced with Bacillus subtilis CGMCC No.29499, all other things being equal.
Example 6
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 2 is that the Bacillus subtilis ACCC60364 is replaced with Bacillus subtilis CGMCC No.29499, all other things being equal.
Example 7
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 1 is that Bacillus subtilis ACCC60364 is replaced with Bacillus megaterium CGMCC No.29498, all the other things being equal.
Example 8
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 2 is that Bacillus subtilis ACCC60364 is replaced with Bacillus megaterium CGMCC No.29498, all the other things being equal.
Example 9
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 1 is that the bacillus subtilis ACCC60364 is replaced with bacillus subtilis CGMCC No.29499 and bacillus megaterium CGMCC No.29498; the microbial agent comprises the following components: bacillus subtilis: bacillus megaterium: paenibacillus mucilaginosus: the ratio of the viable count of Trichoderma harzianum is 20:20:1:2, and all the other viable counts are the same.
Example 10
Special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice
The difference from example 2 is that the bacillus subtilis ACCC60364 is replaced with bacillus subtilis CGMCC No.29499 and bacillus megaterium CGMCC No.29498; the microbial agent comprises the following components: bacillus subtilis: bacillus megaterium: paenibacillus mucilaginosus: the ratio of the viable count of Trichoderma harzianum is 20:20:1:2, and all the other viable counts are the same.
Comparative example 5
Biological compound fertilizer for chemical fertilizer
The difference from example 5 is that no microbial protectant is added, all other things being equal.
Comparative example 6
Biological compound fertilizer for chemical fertilizer
The difference from example 6 is that no microbial protectant is added, all other things being equal.
Comparative example 7
Biological compound fertilizer for chemical fertilizer
The difference from example 7 is that no microbial protectant is added, all other things being equal.
Comparative example 8
Biological compound fertilizer for chemical fertilizer
The difference from example 8 is that no microbial protectant is added, all other things being equal.
Comparative example 9
Biological compound fertilizer for chemical fertilizer
The difference from example 9 is that no microbial protectant is added, all other things being equal.
Comparative example 10
Biological compound fertilizer for chemical fertilizer
The difference from example 10 is that no microbial protectant is added, all other things being equal.
Effect example 1
The high-activity high-concentration chemical fertilizer biological compound fertilizer special for paddy rice prepared in the examples 1-10 is placed for a period of time according to a conventional fertilizer storage mode (normal temperature and dry environment), and the bacterial activity number is changed.
The compound fertilizers prepared in comparative examples 1-10 have viable counts of more than 2 hundred million cfu/g according to the addition amount of microbial agents, but the viable counts of the compound fertilizers prepared in comparative examples 1-10 are less than 0.1 hundred million cfu/g after 3 months.
Table 1 units: hundred million cfu/g
As can be seen from table 1, after a period of time, the number of viable bacteria in the compound fertilizer gradually decreases, the compound fertilizers prepared in example 9 and example 10 decrease the number of viable bacteria most slowly, and the strain stability in the compound fertilizer is higher, probably because bacillus subtilis CGMCC No.29499 and bacillus megaterium CGMCC29498 match to improve the tolerance of all other bacteria to high-concentration fertilizers.
Effect example 2
Examples 1-10 and comparative examples 1-4 for use in rice planting
The application experiments are for example as follows:
to verify the effect of the technology of the present invention, the fertilizers of examples 1 to 10 and comparative examples 1 to 4 of the present invention were subjected to field experiments with commercial fertilizers having common nutrient contents.
Experimental example 1: to verify the effect of the application of the products of examples 1-10 to rice as compared with the comparative examples 1-4, a field test was designed to compare the effects. The test plot is located in a large forest village of pear tree county of Jilin province, the soil is clay salty rice soil, the test result of taking a plough layer soil sample before the test is that the organic matter content is 21.3 g/kg, total nitrogen is 0.97 g/kg, alkaline hydrolysis nitrogen is 120.3 mg/kg, available phosphorus is 37.1 mg/kg, quick-acting potassium is 184 mg/kg, pH value is 7.4, and volume weight is 1.47g/cm 3. The local climate belongs to a semi-humid monsoon climate zone with a warm temperate zone of Liu Xing, and the annual average temperature is 6.6 ℃.
The test set up 15 treatments in total, with treatment 1: common commercial compound fertilizer (nitrogen-phosphorus-potassium content 18-22-6), treatment 2: comparative example 1 product, treatment 3: comparative example 2 product, treatment 4: comparative example 3 product, treatment 5: comparative example 4 product. Treatment 6: example 1 product, treatment 7: example 2 product, process 8: example 3 product, process 9: example 4 product, process 10: example 5 product, process 11: example 6 product, process 12: example 7 product, process 13: example 8 product, process 14: example 9 product, process 15: example 10 product, using cell test, cell area 30m 2, 3 replicates, random block arrangement. Ridge cutting is performed between communities, protection rows are arranged, single-row single irrigation is implemented, and fertilizer stringing between communities is prevented. The test is carried out to compare the fertilizer to be used as the base fertilizer, the dosage is 40kg/667m 2, and other agricultural investment and agricultural operation are consistent except that the comparison fertilizer variety is different among treatments. The rice variety is Ji Hong, the seedling is transplanted in 2021 in 5 months and 12 days, the density is 30 cm multiplied by 14 cm, and 3-4 plants are planted in each hole. Adopts a shallow-wet-shallow intermittent water-saving irrigation method. Harvesting and threshing in cells on 14 days of 10 months. And (3) observing and recording the characteristics of each growth period of the rice in the test period, weighing harvested seeds in cells in the mature period, and collecting the indexes of soil analysis organic matters, nitrogen, phosphorus, potassium nutrient contents, volume weight and the like of the cultivated layer.
The number of tiller and ear forming rate of each treated rice in different growth periods are shown in Table 2.
TABLE 2 Rice tiller number and ear formation Rate for each treatment at different growth periods
As shown by investigation results of the tiller numbers of the treated rice in different growth periods, the difference between the treatments from the transplanting period to the ear alignment period is not large, the tendency of the ear numbers of the mu treated in examples 1-10 to the mature period is higher than that of the mu treated by the comparative example and the common compound fertilizer, the ear numbers of the mu treated in examples are 26.45-28.72 ten thousand plants/667 m 2, the ear numbers of the mu treated in comparative example and the mu treated by the common compound fertilizer are 24.08-25.16 ten thousand plants/667 m 2, the ear forming rate of the mu treated in examples 1-10 is 75.2% -77.1%, the ear forming rate of the common compound fertilizer is 73.55%, and the ear forming rate of the mu treated in examples 1-4 is 70.2% -72.4%. The products of examples 1-10 contain high-activity microbial fertilizer, which can improve rhizosphere soil environment, inhibit the activity of harmful microorganisms, promote early growth and rapid development of seedling root systems, increase effective tillering, promote nutrient absorption of rice, enhance disease resistance and stress resistance of plants, and finally improve the ear forming rate. The fertilizer products of examples 9 and 10 perform relatively well in view of the individual treatment effects of examples 1-10.
The rice yield composition index and yield results in the harvest period are shown in Table 3.
TABLE 3 Rice yield composition index and yield results at harvest stage
From the results of the maturity investigation, the plant height difference between the treatments is not obvious, and the ear length of the treatments in examples 1-10 is higher than that of the treatments in comparative examples 1-4 and the common compound fertilizer. The difference rule among the treatments of the total glume quantity is not obvious, but the spike grain grains show the trend that the treatments of the examples 1-10 are higher than the treatments of the comparative examples 1-4 and the common compound fertilizer, and from the calculation result of the setting rate, the setting rate of the examples 1-10 is 89.4% -92.3%, the setting rate of the comparative examples 1-4 is 78.3% -83.9%, and the setting rate of the common compound fertilizer is 82.3%. The thousand grain weight of each treated rice is not much different and is 24.9-25.5 g. From the final yield results, the rice treated in example 10 gave the highest yield of 706.2kg/667m 2, followed by the treatment in example 9, yields of 703.4kg/667m 2, and the treatments in example 8 and example 7, 696.5kg/667m 2、695.7kg/667m2, respectively. The highest yield of comparative examples 1-4 is 630.9kg/667m 2, the average yield of comparative examples 616.9kg/667m 2 is 626.7kg/667m 2, the yield is close to that of common compound fertilizer treatment, and the yield increase ranges of examples 1-10 are respectively 10.6%, 10.9%, 9.3%, 7.5%, 11.0%, 11.1%, 11.5%, 11.6%, 12.7% and 13.2% by taking the common compound fertilizer treatment as a control, and the statistical analysis results show that the yield increase reaches the significance level.
Results of physical and chemical property assays of each layer of plough soil after harvest are shown in Table 4.
TABLE 4 results of physical and chemical property assays for each layer of plough soil after harvesting
From the soil results, the treatments of examples 1-10 increased the organic matter content of the soil, reduced the volume weight of the soil, and improved the soil environment.
The microbial agent protective agent is added in the preparation process of the products in the examples 1-10, so that the activity of microorganisms is maintained, the metabolic pathway and the metabolic action of ecological flora after the application of soil can improve the plant rhizosphere microecological environment, accelerate the decomposition and conversion of soil organic matters, increase the supply of available nutrients, enhance the metabolic function and disease resistance and disease prevention capability of plants, promote the growth and development of rice, and realize the improvement of yield and quality. The products of examples 9-10 adopt bacillus subtilis CGMCC No.29499 and bacillus megaterium CGMCC29498 to match, can improve the tolerance of live bacteria of microorganisms to high-concentration fertilizers, have higher microbial activity and show relatively better effect in field verification experiments. The products of comparative examples 1-4 have reduced microbial activity without the use of a microbial protectant, and are substantially identical to conventional compound fertilizers in field effect.
The special high-activity high-concentration fertilizer biological compound fertilizer for rice maintains the activity of microorganisms, can improve the plant rhizosphere microecological environment by the metabolic way and the metabolic action of ecological flora after the application of soil, accelerates the decomposition and transformation of soil organic matters, increases the supply of available nutrients, enhances the metabolic function and disease resistance and disease prevention capability of plants, promotes the growth and development of rice, realizes the improvement of yield and quality, and can effectively improve soil and prevent soil hardening.
The above examples of the present invention are merely illustrative of the present invention and are not intended to limit the embodiments of the present invention. Other variations or modifications of the above description will be apparent to those of skill in the art. Not all embodiments are exhaustive. All obvious changes or modifications which come within the spirit of the invention are desired to be protected.
Claims (10)
1. The special high-activity high-concentration chemical fertilizer biological compound fertilizer for rice is characterized by comprising the following components: inorganic nitrogen fertilizer, inorganic phosphate fertilizer, inorganic potash fertilizer, microbial agent and microbial protective agent;
The microbial agent comprises the following components: two or more than two of bacillus subtilis, bacillus mucilaginosus and trichoderma harzianum;
the microbial protectant comprises: brown alginate oligosaccharides, vegetable oil, tetrahydropyrimidine and citric acid;
the inorganic nitrogen fertilizer comprises: ammonium chloride, ammonium sulphate, urea and/or lime nitrogen [ cyanamide based calcium (CaCN 2) ];
The inorganic phosphate fertilizer comprises: monoammonium phosphate, superphosphate and/or ground phosphate rock;
the inorganic potash fertilizer comprises: potassium chloride and/or potassium sulfate.
2. The compound fertilizer of claim 1, wherein the bacillus subtilis is one or more of bacillus subtilis ACCC60364, bacillus subtilis ACCC19743 or bacillus subtilis CGMCC No. 29499;
The bacillus mucilaginosus is bacillus mucilaginosus CGMCC No.17376 and/or bacillus mucilaginosus ACCC10095;
The Trichoderma harzianum is Trichoderma harzianum ACCC33109 and/or Trichoderma harzianum ACCC32072;
preferably, the microbial agent further comprises bacillus megatherium;
preferably, the bacillus megaterium is bacillus megaterium CGMCC No.29498.
3. The compound fertilizer of claim 1, wherein the components of the compound fertilizer further comprise sodium silicate and zinc sulfate.
4. The compound fertilizer of claim 1, which comprises the following components in parts by weight:
15-40 parts of inorganic nitrogen fertilizer, 20-50 parts of inorganic phosphate fertilizer and 10-20 parts of inorganic potash fertilizer;
The microbial agent comprises the following components: the ratio of the number of the viable bacteria of the bacillus subtilis, the bacillus mucilaginosus and the trichoderma harzianum is (35-55): 1 (1-3);
the viable count of the microorganism bacteria in the compound fertilizer is more than 2 hundred million cfu/g;
The mass ratio of the microbial protectant to the microbial inoculant in the compound fertilizer is more than or equal to 1;
the microbial protectant comprises the following components in parts by weight: 65-85 parts of brown alginate oligosaccharides, 5-10 parts of vegetable oil, 5-8 parts of tetrahydropyrimidine and 6-10 parts of citric acid.
5. The compound fertilizer of claim 4, wherein, in the microbial inoculant: the ratio of the viable count of the bacillus subtilis to the viable count of the bacillus mucilaginosus to the viable count of the trichoderma harzianum is 40:1:2;
Preferably, the mass ratio of the microbial protectant to the microbial inoculant in the compound fertilizer is (1-100): 1.
6. The compound fertilizer of claim 4, wherein said vegetable oil comprises: soybean oil and/or castor oil.
7. The compound fertilizer of claim 4, wherein the microbial agent has an effective viable count of greater than 1000 hundred million cfu/gram.
8. The compound fertilizer of claim 4, wherein the inorganic nitrogen fertilizer is urea, ammonium sulfate and/or ammonium chloride; the inorganic phosphate fertilizer is monoammonium phosphate; the inorganic potash fertilizer is potassium chloride and/or potassium sulfate;
Preferably, the compound fertilizer further comprises, by weight: 1-2 parts of sodium silicate and 1-2 parts of zinc sulfate.
9. The method for preparing the special high-activity high-concentration chemical fertilizer biological compound fertilizer for paddy rice according to any one of claims 1 to 8, which is characterized by comprising the following steps:
And uniformly mixing the microbial agent and the microbial protectant, and uniformly mixing with other components to prepare the compound fertilizer.
10. The preparation method of claim 9, wherein the microbial agent and the microbial protectant are uniformly mixed, and then uniformly mixed with other components, and the mixture is granulated to obtain the compound fertilizer.
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