CN117795083A - γδT细胞的工程化及其组合物 - Google Patents

γδT细胞的工程化及其组合物 Download PDF

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Publication number
CN117795083A
CN117795083A CN202280053433.6A CN202280053433A CN117795083A CN 117795083 A CN117795083 A CN 117795083A CN 202280053433 A CN202280053433 A CN 202280053433A CN 117795083 A CN117795083 A CN 117795083A
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cells
population
cell
days
viral vector
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Chinese (zh)
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I·科瓦奇
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Gamade Therapy Co ltd
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Gamade Therapy Co ltd
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K40/41—Vertebrate antigens
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CN202280053433.6A 2021-08-03 2022-08-03 γδT细胞的工程化及其组合物 Pending CN117795083A (zh)

Applications Claiming Priority (3)

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US202163228972P 2021-08-03 2021-08-03
US63/228,972 2021-08-03
PCT/GB2022/052039 WO2023012475A1 (en) 2021-08-03 2022-08-03 Engineering of gamma delta t cells and compositions thereof

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CN117795083A true CN117795083A (zh) 2024-03-29

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US (1) US20240344024A1 (https=)
EP (1) EP4381075A1 (https=)
JP (1) JP2024529015A (https=)
KR (1) KR20240040799A (https=)
CN (1) CN117795083A (https=)
AR (1) AR126690A1 (https=)
AU (1) AU2022322052A1 (https=)
CA (1) CA3228168A1 (https=)
CO (1) CO2024002241A2 (https=)
MX (1) MX2024001657A (https=)
TW (1) TW202328434A (https=)
WO (1) WO2023012475A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119020418A (zh) * 2024-08-17 2024-11-26 广东君厚生物医药有限公司 BaEV逆转录病毒载体的包装方法及其包装细胞系

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4504247A1 (en) 2022-04-04 2025-02-12 Gammadelta Therapeutics Ltd Novel gene armoring

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US6136597A (en) 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
EP1777294A1 (en) 2005-10-20 2007-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins
PT2710123T (pt) 2011-05-19 2017-10-13 Inst De Medicina Molecular Linha celular de linfócitos compreendendo células t gama-delta, método de composição e produção resultante dos mesmos
AU2016274633B2 (en) 2015-06-09 2022-04-21 Gammadelta Therapeutics Ltd Methods for the production of TCR gamma delta+ T cells
WO2017072367A1 (en) 2015-10-30 2017-05-04 Cancer Research Technology Limited EXPANSION OF NON-HAEMATOPOIETIC TISSUE-RESIDENT γδ T CELLS AND USES OF THESE CELLS
DE102017127984B4 (de) * 2017-11-27 2019-12-05 Immatics US, Inc. Verfahren für die Vermehrung und Aktivierung von γδ-T-Zellen
CN113423820A (zh) 2018-11-08 2021-09-21 伽马三角洲疗法有限公司 分离和扩增细胞的方法
GB201818243D0 (en) 2018-11-08 2018-12-26 Gammadelta Therapeutics Ltd Methods for isolating and expanding cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119020418A (zh) * 2024-08-17 2024-11-26 广东君厚生物医药有限公司 BaEV逆转录病毒载体的包装方法及其包装细胞系

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US20240344024A1 (en) 2024-10-17
AU2022322052A1 (en) 2024-02-29
EP4381075A1 (en) 2024-06-12
MX2024001657A (es) 2024-04-19
JP2024529015A (ja) 2024-08-01
TW202328434A (zh) 2023-07-16
CA3228168A1 (en) 2023-02-09
CO2024002241A2 (es) 2024-03-07
AR126690A1 (es) 2023-11-01
KR20240040799A (ko) 2024-03-28

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