CO2024002241A2 - Modificación de linfocitos t gamma delta y composiciones de estos - Google Patents

Modificación de linfocitos t gamma delta y composiciones de estos

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Publication number
CO2024002241A2
CO2024002241A2 CONC2024/0002241A CO2024002241A CO2024002241A2 CO 2024002241 A2 CO2024002241 A2 CO 2024002241A2 CO 2024002241 A CO2024002241 A CO 2024002241A CO 2024002241 A2 CO2024002241 A2 CO 2024002241A2
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Colombia
Prior art keywords
lymphocytes
compositions
modification
gamma delta
cells
Prior art date
Application number
CONC2024/0002241A
Other languages
English (en)
Inventor
Istvan Kovacs
Original Assignee
Gammadelta Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gammadelta Therapeutics Ltd filed Critical Gammadelta Therapeutics Ltd
Publication of CO2024002241A2 publication Critical patent/CO2024002241A2/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4631—Chimeric Antigen Receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464411—Immunoglobulin superfamily
    • A61K39/464412—CD19 or B4
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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    • C12N2510/00—Genetically modified cells
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/12011—Betaretrovirus, e.g. mouse mammary tumour virus
    • C12N2740/12041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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Abstract

La presente invención proporciona métodos para modificar linfocitos T γδ (por ejemplo, linfocitos T vδ1 y linfocitos T vδ2) mediante transducción con un vector viral (por ejemplo, un vector viral con un pseudotipo betaretroviral y una estructura principal de vector viral de la familia Retroviridae 5). Se proporcionan además composiciones de linfocitos T γδ modificados y método de uso de estos.
CONC2024/0002241A 2021-08-03 2024-02-26 Modificación de linfocitos t gamma delta y composiciones de estos CO2024002241A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163228972P 2021-08-03 2021-08-03
PCT/GB2022/052039 WO2023012475A1 (en) 2021-08-03 2022-08-03 Engineering of gamma delta t cells and compositions thereof

Publications (1)

Publication Number Publication Date
CO2024002241A2 true CO2024002241A2 (es) 2024-03-07

Family

ID=83005850

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2024/0002241A CO2024002241A2 (es) 2021-08-03 2024-02-26 Modificación de linfocitos t gamma delta y composiciones de estos

Country Status (9)

Country Link
EP (1) EP4381075A1 (es)
KR (1) KR20240040799A (es)
CN (1) CN117795083A (es)
AR (1) AR126690A1 (es)
AU (1) AU2022322052A1 (es)
CA (1) CA3228168A1 (es)
CO (1) CO2024002241A2 (es)
TW (1) TW202328434A (es)
WO (1) WO2023012475A1 (es)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US6136597A (en) 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
EP1777294A1 (en) 2005-10-20 2007-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins
ES2643387T3 (es) 2011-05-19 2017-11-22 Instituto De Medicina Molecular Estirpe celular de linfocitos que comprende células gama-delta, composición y método de producción de la misma
EP3307875B1 (en) 2015-06-09 2021-12-15 Lymphact - Lymphocyte Activation Technologies, S.A. Methods for the production of tcr gamma delta+ t cells
MY198084A (en) 2015-10-30 2023-07-31 Cancer Research Tech Ltd Expansion of non-haematopoietic tissue-resident ()()t cells and uses of these cells
DE102017127984B4 (de) * 2017-11-27 2019-12-05 Immatics US, Inc. Verfahren für die Vermehrung und Aktivierung von γδ-T-Zellen
KR20210111746A (ko) 2018-11-08 2021-09-13 감마델타 테라퓨틱스 리미티드 세포를 단리 및 증식하는 방법
GB201818243D0 (en) 2018-11-08 2018-12-26 Gammadelta Therapeutics Ltd Methods for isolating and expanding cells

Also Published As

Publication number Publication date
EP4381075A1 (en) 2024-06-12
WO2023012475A1 (en) 2023-02-09
TW202328434A (zh) 2023-07-16
AU2022322052A1 (en) 2024-02-29
AR126690A1 (es) 2023-11-01
CA3228168A1 (en) 2023-02-09
KR20240040799A (ko) 2024-03-28
CN117795083A (zh) 2024-03-29

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