CN1177938C - 选择性连接及扩增方法 - Google Patents

选择性连接及扩增方法 Download PDF

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Publication number
CN1177938C
CN1177938C CNB98804157XA CN98804157A CN1177938C CN 1177938 C CN1177938 C CN 1177938C CN B98804157X A CNB98804157X A CN B98804157XA CN 98804157 A CN98804157 A CN 98804157A CN 1177938 C CN1177938 C CN 1177938C
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primer
segment
sequence
reaction mixture
substantially complementary
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Chinese (zh)
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CN1252104A (zh
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����ɭ��Τ�������е�
艾利森·韦利安·托德
卡罗琳·简·富尔瑞
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Johnson and Johnson Research Pty Ltd
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Johnson and Johnson Research Pty Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB98804157XA 1997-02-21 1998-02-23 选择性连接及扩增方法 Expired - Fee Related CN1177938C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPO5248 1997-02-21
AUPO5248A AUPO524897A0 (en) 1997-02-21 1997-02-21 Method of amplifying specific nucleic acid target sequences

Publications (2)

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CN1252104A CN1252104A (zh) 2000-05-03
CN1177938C true CN1177938C (zh) 2004-12-01

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Family Applications (1)

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CNB98804157XA Expired - Fee Related CN1177938C (zh) 1997-02-21 1998-02-23 选择性连接及扩增方法

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Country Link
US (1) US6245505B1 (https=)
EP (1) EP1002124A4 (https=)
JP (1) JP2001517936A (https=)
KR (1) KR20000075538A (https=)
CN (1) CN1177938C (https=)
AU (1) AUPO524897A0 (https=)
BR (1) BR9807253A (https=)
CA (1) CA2282733A1 (https=)
WO (1) WO1998037230A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104372093A (zh) * 2014-11-10 2015-02-25 博奥生物集团有限公司 一种基于高通量测序的snp检测方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
MXPA01009361A (es) * 1999-03-19 2002-06-04 Genencor Int Placa de pruebas de multiples agujeros pasantes para filtracion de alto rendimiento.
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
EP1313880A2 (en) * 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
AU2003303395A1 (en) * 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
JP2007515956A (ja) * 2003-12-10 2007-06-21 バイオトローブ， インコーポレイテッド 改良された選択的ライゲーションおよび増幅アッセイ
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
WO2005089945A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
WO2005118862A2 (en) * 2004-04-30 2005-12-15 Applera Corporation Compositions, methods, and kits for (mis)ligating oligonucleotides
US7544793B2 (en) * 2005-03-10 2009-06-09 Xialoian Gao Making nucleic acid sequences in parallel and use
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020124A (en) 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
JPH08508636A (ja) * 1993-01-27 1996-09-17 オンコール インコーポレーテッド 核酸配列の増幅
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
IL105894A (en) 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
JP3102800B2 (ja) * 1994-08-19 2000-10-23 パーキン−エルマー コーポレイション 増幅及び連結反応の共役法
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
EP0832280A2 (en) 1995-06-07 1998-04-01 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
CA2199213C (en) * 1996-03-11 2007-04-17 John Wesley Backus Amplifying and detecting target nucleic acids using a post amplification incubation step

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104372093A (zh) * 2014-11-10 2015-02-25 博奥生物集团有限公司 一种基于高通量测序的snp检测方法
CN104372093B (zh) * 2014-11-10 2016-09-21 博奥生物集团有限公司 一种基于高通量测序的snp检测方法
US10704092B2 (en) 2014-11-10 2020-07-07 Capitalbio Corporation Detection methods based on sequencing

Also Published As

Publication number Publication date
JP2001517936A (ja) 2001-10-09
KR20000075538A (ko) 2000-12-15
CA2282733A1 (en) 1998-08-27
US6245505B1 (en) 2001-06-12
WO1998037230A1 (en) 1998-08-27
BR9807253A (pt) 2001-08-28
CN1252104A (zh) 2000-05-03
AUPO524897A0 (en) 1997-03-20
EP1002124A4 (en) 2004-04-14
EP1002124A1 (en) 2000-05-24

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