CN117751875B

CN117751875B - Land-based ecological breeding method for Babylonia and application thereof

Info

Publication number: CN117751875B
Application number: CN202311720563.3A
Authority: CN
Inventors: 刘晓坤; 吕文刚; 张元�; 朱晓闻; 邓岳文; 岳晨阳; 周嘉乐; 常伟成; 潘尚锋; 陈景雷
Original assignee: Hainan Kangguan Biotechnology Co ltd; Guangdong Ocean University
Current assignee: Hainan Kangguan Biotechnology Co ltd; Guangdong Ocean University
Filing date: 2023-12-13
Publication date: 2024-06-28
Anticipated expiration: 2043-12-13

Abstract

The invention discloses a land-based ecological breeding method for Babylonia. The method comprises the following steps: s1, sea water treatment: dropwise adding hydrogen peroxide into the culture pond at a speed of 1 ml to 2 ml per minute; s2, preparing an eastern conch culture pond: the probiotics are more fully mixed between sand layers and at the bottom of the culture pond; s3, throwing in the spiral shell seedling: the anti-stress product is sprayed outside before seedling placement; s4, feeding baits: soaking the bait with the insect-inhibiting essential oil; sprinkling a probiotic; simultaneously feeding aquatic compound vitamins and Li Changkang every day; s5, periodically checking; s6, preparing fermentation nano particles; s7, daily management: and (3) feeding water into the upper layer of the culture pond, draining water below the sand layer, splashing fermentation nano particles for 10-15 days, and supplementing a probiotic agent in the water body. The breeding method obviously improves the number and survival rate of the Babylonia seeds, effectively avoids outbreaks of diseases and can obtain a large number of good-quality and healthy Babylonia seeds.

Description

Land-based ecological breeding method for Babylonia and application thereof

Technical Field

The invention belongs to the technical field of aquaculture, and particularly relates to a land-based ecological aquaculture method for Babylonia and application thereof.

Background

Babylonia is commonly called as a conch, a yellow conch, belongs to the phylum Mollusca (Mollusca), gastropoda (Gastropoda), a front gill subclass (Prosobranchia), a new gastropoda (Neogastropoda), a moth spiroid (Buccinidae) and Babylonia (Babylonia), is distributed in tropical and subtropical areas, is a grazing shallow seafloor perch shellfish, has delicious taste, and is a high-protein and low-fat rare seafood meeting the modern nutritional requirements.

The traditional 11 types of the Babylonia are edible, china is the most dominant country for breeding the Babylonia in the world, and the current breeding of the Babylonia in China is mainly distributed in the coastal areas of the south of Fujian, wherein the Hainan is the most suitable area for breeding and breeding the Babylonia seedlings of China due to the unique geographic environment and climate condition advantages.

At present, the Babylonia is cultivated in a culture pond or a seawater body. If the literature is ' Zhenhanceo ' is a new technology analyse [ J ]. Rural economy and technology, 2020,31 (18): 51-52. ' when the young is enough and a straw are raised, the life habit is converted from floating to attachment after the young starts to be deformed, the feeding habit is also converted from botanic property to animal property, at the moment, the feeding of plankton is stopped, fishes, shrimps, crab meat and the like are fed, the tooth tongue of the just deformed young is just formed, the development is crisp and tender, fresh trash fishes, shrimps and crab meat are fed, the trash fishes are filtered by a 30-mesh screen, the trash fishes are fed with juice, the shrimps and the crabs are fed after being shelled and crushed, 1.0-2.0k parts of animal baits are fed every 100 ten thousand young snails, the feeding amount is gradually increased along with the growth of the young snails, after 2h of feeding, the water quality is kept, and the situation of the young snails can be observed conveniently, and the young snails can be cleaned. (2) The water quality management is carried out once a day, the water exchange amount is 1/3-2/3, the water exchange amount is gradually increased along with the growth of young snails, the bottom sand is required to be cleaned, the sand at the bottom of the pool is required to be gradually supplemented along with the growth of the young snails, and the thickness of the sand layer is preferably consistent with the height of the young snails. (3) When the larvae are deformed into the larvae in daily management, the larvae begin to have wall climbing habit, the larvae below 0.5cm cannot fall into the pond by themselves after climbing, and in order to prevent the larvae from dying after climbing, gauze for soaking pond water should be attached to the inner wall of the larvae cultivation pond, and the larvae on the gauze are flushed into the cultivation pond by pond water before daily feeding.

However, with the increase of the cultivation scale in recent years, the demand of the snail seedlings is larger and larger, the mother snail on the market is updated more slowly, the germplasm is degraded, the quality of the snail seedlings is gradually reduced, the disease resistance is weakened, after the cultivation density and the scale are enlarged, the quality of cultivation water bodies and baits is difficult to ensure, various factors lead the survival rate of the eastern snail standard seedlings in recent years to be very low, the intermediate cultivation and cultivation are more difficult, and the prior cultivation technology cannot achieve the ideal cultivation effect. Therefore, a more mature method for cultivating Babylonia is urgently needed.

Disclosure of Invention

Aiming at the prior art problems, the primary aim of the invention is to provide a land-based ecological breeding method for the Babylonia, which obviously improves the large-specification number and survival rate of the Babylonia seeds, can increase the breeding yield of finished products of the Babylonia, effectively avoids outbreaks of diseases, can obtain a large number of high-quality and healthy Babylonia seeds, and lays a foundation for breeding the Babylonia.

The second object of the invention is to provide an application of the land-based ecological breeding method of the Babylonia in intermediate cultivation of the Babylonia.

In order to achieve the above object, the present invention is realized by the following technical scheme:

A land-based ecological breeding method of Babylonia comprises the following steps:

s1, sea water treatment: pumping the pretreated seawater into a Babylonia culture pond, and dropwise adding hydrogen peroxide at a speed of 1 ml to 2 ml per minute;

S2, preparing an eastern conch culture pond: putting the probiotics with the usage amount of 20-40 g/pond into the Babylonia culture pond for soaking, and draining water within 4-6 hours after the probiotics are just put into the pond, so that the probiotics are more fully mixed between sand layers of the culture pond and the bottom of the pond;

S3, throwing in the spiral shell seedling: the anti-stress product with the dosage of 5 to 10ppm is sprayed outside before the seedling is put in, the Babylonia spiral shell seedling and the shrimp seedling for cleaning the residual bait are put in, and the anti-stress product is sprayed outside continuously for 2 days after the seedling is put in, twice a day;

S4, feeding baits: before bait feeding, the bait is soaked by the insect-inhibiting essential oil according to the dosage of 0.2-1.0 mL/5 jin of bait; after ingestion is completed, increasing the drainage amount, and splashing the water with the probiotic agent with the usage amount of 3-8 ppm after the water becomes clear; meanwhile, 1-5 g/jin of aquatic compound vitamin bait and 2-5 g/jin of Lichangkang bait are fed every day;

S5, periodically checking: periodically detecting, namely knowing the water quality condition and performing spot check on the Babylonia and the water quality;

s6, preparing fermentation nano particles: fermenting the porous nano-particles mixed with EM bacteria to obtain fermented nano-particles;

S7, daily management: the upper layer of the culture pond continuously feeds water for 24 hours, the sand layer is drained, dirty water is drained completely during feeding, the daily feeding and draining are kept to be 20%, the old water is kept to be 80%, 10-20 g of fermentation nano particles are uniformly sprayed on every square meter for 7-15 days, and meanwhile, the water body is supplemented with the probiotics with the dosage of 0.1-0.5 ppm, so that the Babylonia is cultivated.

The land-based ecological breeding method for the Babylonia has the advantages that the number and the survival rate of the Babylonia seedlings are remarkably improved, the outbreak of diseases is effectively avoided, and a large number of high-quality and healthy Babylonia seedlings can be obtained. Specifically:

According to the invention, the seawater is treated by the hydrogen peroxide, so that the oxygen deficiency symptom in rainy weather can be effectively relieved by the hydrogen peroxide, active oxygen is generated after the water is dissolved, the dissolved oxygen of the water is increased, the oxygen deficiency of Babylonia is prevented, and the effect of improving the water quality is achieved; in addition, the hydrogen peroxide can degrade harmful substances such as algae toxins, ammonia nitrogen, nitrous acid, hydrogen sulfide and the like, relieve the phenomena of whitening, stinking and the like of the water body, have the effects of running water and refreshing water, and create good growth and living conditions for the Babylonia. And the hydrogen peroxide can not form toxic byproducts in the water body, so that the safety is extremely high. In addition, the inventor finds that when the dropping speed of the hydrogen peroxide is high, the subsequent cultivation of the Babylonia seedlings can be influenced; when the dropping speed of the hydrogen peroxide is low, the above-mentioned accelerating effect is hardly achieved. And when the dropping speed of the hydrogen peroxide is 1 ml to 2 ml per minute, the promotion effect can be well maintained, and the subsequent cultivation of the Babylonia seedlings can not be influenced.

The culture method provided by the invention discharges pool bottom water (namely, the water is discharged from the lower part of the sand layer) when the water is specifically discharged, so that the beneficial bacteria content of the sand layer of the pool is increased and durable, the number of harmful bacteria in the water body is greatly reduced, the sand layer can be kept clean for a long time, the problems that the sand layer is easy to blacken and smell, the eastern snail is easy to attack and the like are reduced, the prevention measures are in place, and the probability of occurrence of the eastern snail seedlings is reduced.

In addition, the invention adopts porous nanoparticle mixed EM bacteria for fermentation, thus obtaining unique fermentation nanoparticles, and compared with the traditional full-tank direct splashing, the method has the advantages that the retention time of beneficial bacteria in sand is longer, and the indexes of ammonia nitrogen, nitrous acid and the like of the water body are always kept in a lower range.

The cultivation method provided by the invention keeps water inflow and drainage of 20% every day, keeps old water of 80%, can keep the stability of water, creates a more stable water environment for the Babylonia, can reduce uncertainty such as stress and the like, and reduces the incidence rate of Babylonia seedlings.

According to the invention, the probiotics, the anti-stress product, the aquatic compound vitamin, the Lichangkang and the like are applied to cooperate with each other in the process of breeding the Babylonia, so that the physique of the Babylonia seedling is enhanced, the enteritis is reduced, the outbreak of diseases is effectively avoided, and the health of the Babylonia seedling is ensured.

Preferably, in step S1, the pretreatment operation of the pretreated seawater is:

(1) Sea area selection: the water quality of the sea area must meet the water quality standard of the first type or the second type of the national culture sea water;

(2) Well digging and water pumping: the deep water well under the coast sand layer is constructed, so that the deep water well is lower than the minimum tide level in the whole year, the deep water well is guaranteed to be always submerged by seawater, and water can be pumped for 24 hours in an all-weather manner;

(3) And (3) sedimentation in an external pond: pumping up seawater to precipitate in the external pond for 12-36 hours, and pumping only upper water;

(4) Primary filtering: the bottom layer of the pond is fully paved with sand, a pipe is buried under the sand layer, and large particles in the natural seawater are filtered by the sand;

(5) Secondary filtration: an activated carbon filter cotton capable of removing particulate matters with the particle size larger than 1mm in the seawater is arranged for further removing tiny particulate matters, protozoa and the like;

(6) Sterilizing seawater: sterilizing by ultraviolet rays to obtain qualified water for cultivating the snails;

(7) Storage of seawater: pumping the treated seawater into a water storage tank, wherein the storage time is not longer than 3 days;

preferably, in the step S2, the soaking time of the probiotic is 12-48 hours.

Preferably, in the step S2, the drainage lasts for 5 to 10 seconds. Drainage is carried out for 4-6 hours after the probiotic agent is put in, and the drainage is controlled to last for 5-10 seconds, so that the probiotic agent is more fully fused between sand layers and at the bottom of the pool.

Preferably, in the step S2, the probiotic includes bacillus complex and vibrio phagocytosis, and the bacillus complex includes bacillus subtilis and bacillus licheniformis. More specifically, the total number of effective bacteria of the bacillus compoundus is more than or equal to 5.0X10 ⁸ cfu/ml, and the total number of effective bacteria of vibrio phagostimulans is more than or equal to 4.7X10 ¹⁰ pfu/ml. In the present invention, bacillus subtilis and bacillus licheniformis may be combined in any ratio.

Preferably, in the step S3, the anti-stress product is sprayed out 10-60 min before seedling placement.

Preferably, in the step S3, the anti-stress product is selected from one or more of astaxanthin, glucose, and vitamin C.

Preferably, in the step S4, the insect-inhibiting essential oil includes, in parts by weight: 4-5 parts of chinaberry essential oil, 3-4 parts of eucalyptus essential oil, 1-2 parts of lavender essential oil, 1-2 parts of marigold essential oil and 1-2 parts of mint essential oil.

Preferably, in the step S4, the aquatic compound vitamin comprises 1.5-10 parts by weight of vitamin B1, 2-12 parts by weight of vitamin B2, 1-8 parts by weight of vitamin B6, 0.3-1 part by weight of vitamin B12, 3-15 parts by weight of D-calcium pantothenate, 6-20 parts by weight of nicotinamide, 0.4-2 parts by weight of folic acid, 1.5-10 parts by weight of vitamin A, 1-3 parts by weight of vitamin D3, 1.5-10 parts by weight of vitamin E, 1.2-5 parts by weight of vitamin K3, 4-10 parts by weight of vitamin C, 0.1-1.5 parts by weight of D-biotin, 10-30 parts by weight of dibutyl hydroxytoluene (BHT) and 20-40 parts by weight of steady state powder.

Preferably, in the step S4, the li chang kang includes, in parts by weight: 4-5 parts of tannic acid, 2-3 parts of lactobacillus casei, 2-3 parts of oligosaccharide and 3-4 parts of enterococcus faecium.

Preferably, in the step S4, the bait includes artemia nauplii, oysters and crabs, squid and trash fish, etc.

Preferably, in the step S4, artemia nauplii are fed with 0.3-0.5 g/m ² in the first four days of initial young snail larvae. Oyster starts to be added on the fifth day, and artemia nauplii are calculated according to parts by weight: oyster from day five to day eight was 8: 2. 6: 4. 4: 6. 2:8. all the larvae are changed into oysters on the ninth day, the oysters last for 20 to 40 days, and after the intermediate cultivation stage is finished, the larvae reach the larvae from the larvae, and the larvae are changed into the larvae for feeding the trash fish, the squid, the crabs and the like.

Preferably, in the step S6, the porous nanoparticle is a calcium carbonate nanoparticle. The particle size of the porous nano particles is 1-3 mm.

Preferably, in the step S6, the mass ratio of the porous calcium carbonate particles to the EM bacteria is 2-4: 1.

Preferably, the Babylonia seedlings are young snails which fall to the bottom and are deformed for 2-7 days, have good health and activity, are not damaged and have uniform size, and the seedling density is controlled to be 0.8-1.3 ten thousand per m ²; or the young snail with the density of more than 0.5cm after intermediate cultivation is controlled to be 0.1-0.2 ten thousand per m ².

Preferably, the shrimp larvae are larvae of 3 to 7 days, the shrimp larvae are SPF first-generation or second-generation shrimp larvae which are good in reverse water property and free of carrying viruses and vibrios, and the stocking density is 10 to 100 tails/m ².

Furthermore, the invention also discloses application of the land-based ecological breeding method of the Babylonia in intermediate cultivation of the Babylonia.

Compared with the prior art, the invention has the following beneficial effects: the invention provides a land-based ecological breeding method for Babylonia, which can be well applied to intermediate cultivation of Babylonia. The land-based ecological breeding method can remarkably improve the growth rate (the amount of the snails) and the survival rate of the Babylonia seeds, effectively avoid outbreaks of diseases, obtain a large number of high-quality and healthy Babylonia seeds, and lay a foundation for breeding the Babylonia.

Drawings

FIG. 1 is a schematic diagram of the suspension hydrogen peroxide of the present invention.

Fig. 2 is a schematic illustration of a laid base plate and base net.

Fig. 3 is a schematic view of a conventional net hanging form.

Fig. 4 is a schematic view of an improved screen form.

Fig. 5 is a front view of the sprinkler.

Fig. 6 is a side view of the spray device.

FIG. 7 is a schematic view of blackening sand in a screw pond during cultivation.

FIG. 8 is a schematic view of normal sand in a pond during cultivation.

FIG. 9 is a schematic representation of harvesting commodity snails in example 1.

FIG. 10 is a schematic diagram of a spiro Chi Luomiao during an intermediate cultivation.

FIG. 11 is a schematic diagram of intermediate-cultivated seedlings harvested in example 2.

FIG. 12 is a schematic diagram of Vibrio detection; wherein, the left side is a diagram of unused culture dishes (containing culture medium), the middle is a diagram of Vibrio aquaticus content in comparative example 7, and the right side is a diagram of Vibrio aquaticus content in example 2.

Detailed Description

The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.

Example 1 land-based ecological cultivation method of Babylonia

S1, sea water treatment

(3) And (3) sedimentation in an external pond: pumping up seawater to precipitate in the external pond for 24 hours, and pumping only upper water;

(8) Feeding water into a spiral pool: pumping seawater in the water storage tank into the spiral pool, hanging a bottle of hydrogen peroxide (shown in figure 1) at the water outlet of the water storage tank, dropwise adding 1 ml to 2 ml per minute into the spiral pool, and dropwise adding for 24 hours.

S2, pool preparation

As shown in fig. 2, 3 and 4, the bottom plate and the gauze are paved on the bottom of the spiral pool, and then the hanging net and the matrix are paved. The hanging net is 60 meshes, the intersection with the water surface is provided with escape-preventing burrs, and the ratio of the purse net to the water surface is 2:8, reserving water around as a buffer zone; the matrix is gravel and shell powder with the thickness of 1-2 mm and the thickness of 5 cm. As shown in fig. 5 and 6, the sprinkler includes a water inlet ball valve, a structural support, and a sprinkler structure; the spraying structure consists of water pipes around the pond and 2-3 water pipes in the middle, the water pipes are provided with a plurality of tiny water holes, and the diameter of the water pipes is 1-3 cm; the structural support is a U-shaped buckle and is formed by connecting one or more of the U-shaped buckles side by side; the spraying structure is hung on a structural support frame on the side of the pond or on the side of the cultivation pond.

10Ppm povidone iodine is used for disinfecting sand, a hanging net and a spiral pool one week before seedling placement, water is drained after soaking for 2 days, and water is added again for cleaning. 2 days before seedling placement, the water level is reduced to be 10 centimeters away from the substrate, a probiotic is put in (the use amount of the probiotic is 40 grams per pond, the probiotic comprises composite bacillus and vibrio, the composite bacillus comprises bacillus subtilis and bacillus licheniformis, wherein the total number of effective bacteria of the composite bacillus is more than or equal to 5.0 multiplied by 10 ⁸ cfu/ml, the total number of effective bacteria of the vibrio is more than or equal to 4.7 multiplied by 10 ¹⁰ pfu/ml), the probiotic is soaked for 48 hours, and water is drained for 6 seconds after the probiotic is just put in, so that the probiotic is more fully fused between sand layers and at the bottom of the pond.

S3, seedling throwing

Carefully selecting the snail seedlings, strictly executing a quarantine system, and putting the seedlings after the snail seedlings are qualified. The young snails with 0.5 to 1.5 cm of good health and activity, no damage and uniform size are selected from the young snails; quarantine system is that the snail seedlings do not carry sporidium and vibrio; the seedling density is controlled to be 2.3 ten thousand per 16m ².

8Ppm of anti-stress product (product name: activated, guangzhou gold aquatic animal health care products Co., ltd.) is sprayed out 30min before seedling placement, the dosage is 5-10 ppm, the quality of water in a spiral pool is taken as a reference, the following is referred to as ppm and the like), and the product is sprayed out for 2 days after seedling placement, twice a day. Meanwhile, P3-P7 stage shrimp larvae (the shrimp larvae are SPF first-generation or second-generation shrimp larvae which are good in reverse water property and do not carry viruses and vibrios, the stocking density is 50 tails/m ²) are put in, the shrimp larvae are used for cleaning residual baits, and the snail larvae and the shrimp larvae are all cleaned after being soaked in 6ppm hydrogen peroxide solution for 10 seconds before being put in and then are put in a pool.

S4, feeding baits

During the cultivation period, the daily feeding amount accounts for 5% -15% of the total weight of the snails, the feeding is completed within 1.5 hours, the early specification is smaller than 100 heads/jin, the feeding is stopped for 1 day after 2 days, the feeding is performed once in two days when the later specification is larger than 100 heads/jin, the feeding is selected in the evening, the bait is mainly mixed fish, the feeding amount is increased along with the growth of the snails, and the water is stopped during feeding and the residual bait collecting. Before feeding the bait, the bait is soaked for 10-20 minutes by using insect-inhibiting essential oil (the insect-inhibiting essential oil consists of 5 parts by weight of chinaberry essential oil, 4 parts by weight of eucalyptus essential oil, 2 parts by weight of lavender essential oil, 1 part by weight of marigold essential oil and 1.5 parts by weight of peppermint essential oil) according to 0.5ml/5 jin of the bait. Before bait feeding, 5-7 ppm hydrogen peroxide solution is used for cleaning and soaking for 10-15 seconds, so that the carrying amount of harmful heterotrophic bacteria is reduced. After the ingestion is completed, the water discharge is increased, and 3-8 ppm of the probiotic is used for sprinkling until the water becomes clear for 5-8 hours. Meanwhile, 1-5 g/jin of aquatic compound vitamin bait and 2-5 g/jin of Lichangkang are added every day for feeding, the constitution of the spiral seedlings is enhanced, enteritis is reduced, and 0.1-0.4 egg white is used for wrapping the medicine for each kilogram of bait during medicine mixing;

Wherein, the aquatic compound vitamin comprises 5 parts of vitamin B1,7 parts of vitamin B2,5 parts of vitamin B6,0.5 part of vitamin B12, 10 parts of D-calcium pantothenate, 16 parts of nicotinamide, 1.5 parts of folic acid, 4 parts of vitamin A,2 parts of vitamin D3,6 parts of vitamin E,3 parts of vitamin K3,6 parts of vitamin C,0.4 part of D-biotin, 20 parts of dibutyl hydroxy toluene (BHT) and 30 parts of handrail powder according to parts by weight.

The Lichangkang comprises 5 parts of tannic acid, 3 parts of lactobacillus casei, 2 parts of oligosaccharide and 3 parts of enterococcus faecium in parts by mass.

S5, periodically detecting

Periodically detecting, knowing the water quality condition, carrying out spot check on the Babylonia and the water body once for 2-5 days, detecting whether parasites (such as sporozoons, wheel worms, ciliates, dactylogyrus, ichthyophthirius, hawk-bone and the like) exist on the surfaces and in the bodies of the Babylonia, detecting the content of vibrio (such as vibrio parahaemolyticus, vibrio harveyi, vibrio alginolyticus and the like) in the water body, and carrying out targeted control according to the detection result.

S6, fermenting nano particles

Porous calcium carbonate particles with the particle size of 1-3 mm and EM bacteria are mixed according to the mass ratio of 2:1, mixing and fermenting to obtain the fermented nano-particles.

S7, daily management

The upper layer of the culture pond continuously feeds water for 24 hours, the sand layer is drained, the daily feeding and draining are kept at 20%, the old water is kept at 80%, in addition, the dirty water in feeding is completely drained, 10-20 g of fermentation nano particles are uniformly sprayed every square meter every 10-15 days, and meanwhile, 0.1-0.5 ppm of probiotics is supplemented in the water body.

S8, problem processing

(1) For ponds with turbid water body, serious blackening of sand (shown in figure 7) and no sand drilling of Babylonia, black sand silt is dug manually, 40 ml/pond of hydrogen peroxide is splashed every 4 hours, the sand can be obviously whitened after two days of continuous use, and the water body is not turbid any more (shown in figure 8);

(2) The content of vibrio exceeds the standard, and after 5ppm povidone iodine is cleaned and disinfected for 2 hours, the water is increased to change the disinfectant;

(3) The parasite on the spiral shell exceeds standard, after closing running water, first using hydrogen peroxide 0.5-0.7 g/m3 to synergize, medicated bath for 2 hours, then using insecticide 1-3 times (trichlorfon/copper sulfate, the dosage is 0.1-3.0 ppm, using 1-2 days later the whole pond is sprayed with organic acid to detoxify), can effectively inhibit and kill cysts and parasites in the spiral shell pond, the water temperature is not more than 30 ℃, and the effect is affected;

(4) The material is controlled in rainy days, and the material is not fed or is fed only once a day, in particular to a pool without rainproof measures, and the material is not fed.

S9, cultivating

Cultivated to commodity snail for 6 months (as shown in fig. 9).

Example 2

The difference between this embodiment and embodiment 1 is that: and step S4, feeding the feed for 20-40 g/ten thousand in the early stage and 180-200 g/ten thousand in the later stage, wherein the feed is suitable for 2 hours, and the aeration and the flowing water are stopped during the feeding period. Feeding artemia nauplii in the first four days of seedling placement, 0.3-0.5 g/m ², adding oyster in the fifth day, and counting the mass parts of artemia nauplii: oyster from day five to day eight was 8: 2. 6: 4. 4: 6. 2:8, starting from the ninth day, all the larvae are changed into oysters, the feeding artemia nauplii are evenly sprayed after being extracted by using a reciprocating stainless steel spray rod, and the oysters are fed after being crushed by a manual crusher, and are fed once in the morning and at the evening. In the step S9, the medium cultivation is carried out for 25 days (as shown in fig. 10 and 11), the S3 snail seedlings are young snails which are in ground metamorphosis for about 5 days and 1mm, and the seedling placing density is controlled to be 1.1 ten thousand per m ².

Example 3

The difference between this embodiment and embodiment 1 is that:

the insect-inhibiting essential oil consists of, by mass, 4 parts of chinaberry essential oil, 3 parts of eucalyptus essential oil, 1 part of lavender essential oil, 2 parts of marigold essential oil and 1 part of mint essential oil.

The aquatic compound vitamin comprises, by mass, 1.5 parts of vitamin B1, 12 parts of vitamin B2,1 part of vitamin B6,1 part of vitamin B12,3 parts of D-calcium pantothenate, 6 parts of nicotinamide, 2 parts of folic acid, 1.5 parts of vitamin A,3 parts of vitamin D3, 10 parts of vitamin E,1.2 parts of vitamin K3, 10 parts of vitamin C,0.1 part of D-biotin, 10 parts of dibutylhydroxytoluene (BHT) and 40 parts of handrail powder.

The Lichangkang comprises 4 parts of tannic acid, 2 parts of lactobacillus casei, 3 parts of oligosaccharide and 4 parts of enterococcus faecium in parts by mass.

Example 4

The difference between this embodiment and embodiment 1 is that:

The insect-inhibiting essential oil consists of 4.5 parts of chinaberry essential oil, 3.5 parts of eucalyptus essential oil, 1.5 parts of lavender essential oil, 1 part of marigold essential oil and 2 parts of mint essential oil according to parts by weight.

The aquatic compound vitamin comprises, by mass, 10 parts of vitamin B1,2 parts of vitamin B2,8 parts of vitamin B6,0.3 part of vitamin B12, 15 parts of D-calcium pantothenate, 20 parts of nicotinamide, 0.4 part of folic acid, 10 parts of vitamin A,1 part of vitamin D3,1.5 parts of vitamin E,5 parts of vitamin K3,4 parts of vitamin C,1.5 parts of D-biotin, 30 parts of dibutylhydroxytoluene (BHT) and 20 parts of handrail powder.

Comparative example 1

The difference between this comparative example and example 1 is that: in the step S1 of seawater treatment (8), hydrogen peroxide is not added.

Comparative example 2

The difference between this comparative example and example 1 is that: in the step S4, bait feeding is carried out without adding insect-inhibiting essential oil.

Comparative example 3

The difference between this comparative example and example 1 is that: in the step S4, bait is fed without adding the Lichangkang.

Comparative example 4

The difference between this comparative example and example 1 is that: in the step S6, the same amount of EM bacteria is directly splashed into the culture pond without adding fermentation nano particles in daily management.

Comparative example 5

The difference between this comparative example and example 1 is that: in step S7, no probiotic is added for daily management.

Comparative example 6

The difference between this comparative example and example 1 is that: the conventional cultivation is that hydrogen peroxide, insect-inhibiting essential oil, lichangkang, fermentation nano-particles and probiotics are not added.

Comparative example 7

The difference between this comparative example and example 2 is that: in the step S9, the medium cultivation is carried out for 25 days, and the conventional cultivation is carried out without adding hydrogen peroxide, insect-inhibiting essential oil, lichangkang, fermentation nano-particles and probiotics.

Test example 1

The above examples and comparative examples were tested using the following test methods:

(1) The ammonia nitrogen content, nitrous acid content and pH value were tested using a kit from Guangzhou gold aquatic animal health products Co.

(2) Spore worm detection: 5 Babylonia are randomly extracted from the pool, the shell of the Babylonia is broken, the intestinal tract is taken and put on a glass slide, and the observation is carried out under a microscope.

(3) Average specification: randomly weighing 3 times of 5 jin of Babylonia, and calculating the average quantity per jin; the calculation method comprises the following steps: average specification= (first number of threads+second number of threads+third number of threads)/(3/5) (head/jin).

(4) Survival rate: the percentage of the number of the surviving snails and the number of the Babylonia seedlings in the whole culture period; the calculation method comprises the following steps: survival = (number of seedlings released-number of death)/number of seedlings released x 100%.

(5) The screw with bad state such as turning over, swelling of the anastomat tube, swelling of the gastropod, hardening of the gastropod, etc. and the screw touching the motionless gastropod and the screw with empty shell should be picked up in time, and all the screw should be counted as death.

(6) Vibrio detection test of water: the culture medium used for vibrio detection is thiosulfate citrate bile salt sucrose agar culture medium, namely TCBS agar culture medium. The vibrio detection steps are as follows: ① taking a water sample. Firstly, 200 milliliters of water sample to be detected is taken and placed in a shade, and if the water sample is not detected temporarily, the water sample is stored in the shade for no more than 4 hours. ② Pour agar plates. One conical flask was taken and 72 grams of TCBS agar was added per 800 milliliters of water. Heating the conical flask on an electric furnace, quickly taking up the conical flask with cloth after the conical flask is heated to be boiled, putting the conical flask on the electric furnace after foam sinks, and repeatedly performing the actions for 3 times to turn off the electric furnace and putting the conical flask on an ultra-clean workbench for cooling. After cooling for 20 minutes, the dish was left in half empty, an alcohol lamp was placed under it, and the plate was poured over the flame of the alcohol lamp (to maintain a sterile ring). The agar from the conical flask is poured into a plate (preferably, the agar just spreads over the plate, and the thickness of the plate is moderate). After the flat plate is poured, the culture dish is covered, and the contamination of the mixed bacteria is prevented. And (5) coating a water sample after the flat plate is completely cooled and hardened. ③ The water sample was applied by pipetting the water sample with a pipette and 0.1 ml was applied to the plate of the petri dish and the water sample was evenly applied to the plate with an applicator rod. After the coating, the dishes are placed in an incubator (temperature 32 ℃) for culturing for 12 to 16 hours, and if the incubator is not used, a culturing time of 18 to 24 hours is required at room temperature. The number of yellow-green bacteria can be counted. ④ The yellow-green bacteria colonies on the flat plate are counted by counting and the yellow-green bacteria content, and scribing counting can be adopted when the number is large.

FIG. 12 is a schematic diagram of Vibrio detection; wherein, the left side is a diagram of unused culture dishes (containing culture medium), the middle is a diagram of Vibrio aquaticus content in comparative example 7, and the right side is a diagram of Vibrio aquaticus content in example 2. As shown in FIG. 12, the number of vibrios in the water body treated by the method of the invention is remarkably reduced.

The final test data of the above examples and comparative examples are shown in table 1 below.

TABLE 1

As shown in the above Table 1, the Babylonia in example 1 has the best cultivation effect, the average specification is 45-50, the yield reaches 418 jin, and the survival rate reaches 91.2%. The three physiological indexes of ammonia nitrogen, nitrous acid and pH value are kept at low concentration, and no sporozoon exists in the intestinal tract. In contrast, in the Babylonia cultures of comparative examples 1 to 7, the ammonia nitrogen content, the nitrous acid content and the pH value are all high, and the Babylonia cultures are unstable and have large fluctuation.

The invention adopts the hydrogen peroxide to effectively relieve the anoxic symptom in rainy weather, generates active oxygen after dissolving the water body, increases the dissolved oxygen of the water body, prevents the oxygen deficiency of the Babylonia and plays a role in improving the water quality; in addition, the hydrogen peroxide can degrade harmful substances such as algae toxins, ammonia nitrogen, nitrous acid, hydrogen sulfide and the like, relieve the phenomena of whitening, stinking and the like of the water body, have the effects of running water and refreshing water, and create good growth living conditions for aquatic animals. And the hydrogen peroxide can not form toxic byproducts in the water body, so that the safety is extremely high. As can be seen from the examples 1 and 1, the average specification and the screw quantity of the final cultivated Babylonia are obviously improved and the survival rate of the Babylonia is obviously improved after the seawater is treated by hydrogen peroxide.

As can be seen from the examples 1, 2 and 3, the adoption of the insect-inhibiting essential oil and the Lichangkang in the process of breeding the Babylonia can obviously improve the average specification and the screw quantity of the Babylonia, and the survival rate is also increased to a certain extent. The insect-inhibiting essential oil and the Lichangkang can effectively prevent enteritis and sporozoosis, can efficiently optimize intestinal flora of the Babylonia, enhance digestion and absorption of the Babylonia, promote ingestion, promote intestinal peristalsis and improve intestinal health level of the Babylonia larvae. In addition, the growth of the Babylonia can be promoted, the immunity of the Babylonia can be improved, and the morbidity can be reduced.

As can be seen from example 1 and comparative example 4, the present invention uses unique fermented nanoparticles to allow the beneficial bacteria to remain in the sand for a longer period of time than the traditional full pool direct splashing of the beneficial bacteria. The probiotics can supplement beneficial bacteria in the water body, so that the ecological position of harmful bacteria is preempted, the propagation and growth of the harmful bacteria can be directly or indirectly inhibited, and the cultivation cost is reduced. The invention adopts the fermentation nano particles, so that the average specification and the screw quantity of the final cultivated Babylonia are obviously improved, and the survival rate of the Babylonia is obviously improved.

The conventional cultivation method is adopted in the comparative example 6, hydrogen peroxide, insect-inhibiting essential oil, lichangkang, fermentation nano particles and probiotics are not added, the cultivation effect is the worst, the indexes such as survival rate and yield are the worst, the insect-inhibiting essential oil is not used in the comparative example 2 and the comparative example 6, and the Babylonia grows in the intestinal tract of the Babylonia.

Example 2 and comparative example 7 are intermediate cultures of Babylonia (incubation time 25 days). The survival rate of the Babylonia in the example 2 reaches 94.6 percent which is remarkably higher than that of the Babylonia in the comparative example 7 by 53.8 percent, the large-scale yield difference is large, the Babylonia in the example 2 has 134 jin of weight in 1200-1500 scale, which is 3.5 times of the yield of the Babylonia in the comparative example 7, and ammonia nitrogen, nitrous acid and the like can also keep low concentration. This shows that the land-based ecological breeding method of the Babylonia is very suitable for the intermediate breeding of the Babylonia.

The water can be fed and discharged 20% per day, the old water can be kept 80%, and the stability of the water body can be kept. Under the combined action of the hydrogen peroxide, the insect-inhibiting essential oil, the Lichangkang, the fermentation nano particles, the probiotics and the like, a more stable and safer water environment is created for the Babylonia, physiological indexes such as ammonia nitrogen, nitrous acid and the like of the water body and the sand layer are ensured, the normal range is maintained, the uncertainty of stress and the like possibly caused by new water is reduced, the morbidity is reduced, and the healthy and sustainable development of the Babylonia breeding industry is promoted.

The foregoing examples are illustrative only and serve to explain some features of the method of the invention. The claims that follow are intended to claim the broadest possible scope as conceivable and the embodiments presented herein are demonstrated for the applicant's true test results. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.

Claims

1. The land-based ecological breeding method for the Babylonia is characterized by comprising the following steps of:

s7, daily management: continuously feeding water into the upper layer of the culture pond for 24 hours, draining water below the sand layer, simultaneously, fully draining dirty water during feeding, keeping 20% of water fed and drained every day, keeping 80% of old water, uniformly splashing 10-20 g of fermentation nano particles per square meter for 7-15 days, and simultaneously supplementing a probiotic agent with the dosage of 0.1-0.5 ppm into a water body to cultivate and obtain Babylonia;

the probiotics comprise composite bacillus and vibrio phagocytosis, and the composite bacillus comprises bacillus subtilis and bacillus licheniformis;

The insect-inhibiting essential oil comprises the following components in parts by weight: 4-5 parts of chinaberry essential oil, 3-4 parts of eucalyptus essential oil, 1-2 parts of lavender essential oil, 1-2 parts of marigold essential oil and 1-2 parts of mint essential oil;

The Lichangkang comprises the following components in parts by weight: 4-5 parts of tannic acid, 2-3 parts of lactobacillus casei, 2-3 parts of oligosaccharide and 3-4 parts of enterococcus faecium.

2. The land-based ecological breeding method of Babylonia according to claim 1, wherein the anti-stress product is one or more selected from astaxanthin, glucose and vitamin C.

3. The land-based ecological breeding method of Babylonia according to claim 1, wherein in the step S4, the aquatic compound vitamin comprises 1.5-10 parts by weight of vitamin B1, 2-12 parts by weight of vitamin B2, 1-8 parts by weight of vitamin B6, 0.3-1 part by weight of vitamin B12, 3-15 parts by weight of D-calcium pantothenate, 6-20 parts by weight of nicotinamide, 0.4-2 parts by weight of folic acid, 1.5-10 parts by weight of vitamin A, 1-3 parts by weight of vitamin D3, 1.5-10 parts by weight of vitamin E, 1.2-5 parts by weight of vitamin K3, 4-10 parts by weight of vitamin C, 0.1-1.5 parts by weight of D-biotin, 10-30 parts by weight of dibutylhydroxytoluene and 20-40 parts by weight of supporting powder.

4. The land-based ecological breeding method of Babylonia according to claim 1, wherein in the step S4, the bait comprises artemia nauplii, oysters, crabs, squid and trash fish.

5. The land-based ecological breeding method of the Babylonia according to claim 1, wherein the Babylonia seedlings are young snails which fall to the bottom and are abnormal for 2-7 days, are healthy and active, have no damage and have uniform sizes.

6. The land-based ecological breeding method of Babylonia according to claim 1, wherein the young shrimp is young shrimp of 3-7 days, and the young shrimp is young shrimp of SPF generation or second generation with good reverse water property and no carrying virus and vibrio.

7. Use of the land-based ecological breeding method of Babylonia according to any one of claims 1 to 6 in intermediate cultivation of Babylonia.