CN117731581A

CN117731581A - Hair loss preventing composition with scalp care function, application thereof, hair washing product and preparation method of hair washing product

Info

Publication number: CN117731581A
Application number: CN202311761556.8A
Authority: CN
Inventors: 周勤; 肖瑞光; 许桂萍
Original assignee: Zhaoqing Dicai Daily Chemical Science And Technology Co ltd
Current assignee: Zhaoqing Dicai Daily Chemical Science And Technology Co ltd
Priority date: 2023-12-19
Filing date: 2023-12-19
Publication date: 2024-03-22

Abstract

The application relates to the field of cosmetics, in particular to a composition with scalp care and hair loss prevention functions, application of the composition, a shampoo product and a preparation method of the shampoo product. An anti-hair loss composition with scalp care, comprising, in mass percent: 1.0 to 35 percent of ginger juice, 0.01 to 2.0 percent of allantoin, 0.1 to 10.0 percent of panthenol, 0.1 to 5 percent of cacumen biotae extract, 0.005 to 0.1 percent of hydrolyzed chitin and 0.01 to 0.5 percent of salicylic acid; the balance being water. The anti-hair loss composition for scalp care provided by the application realizes the synergistic effect by the synergistic cooperation of the components; the effects of the components are maximized, scalp is nursed from various paths of scalp hair follicles and scalp inflammations and multiple dimensions, so that the scalp is nourished and healthier, the optimal ideal state of anti-alopecia effect is achieved, and the anti-alopecia effect is finally achieved.

Description

Hair loss preventing composition with scalp care function, application thereof, hair washing product and preparation method of hair washing product

Technical Field

The application relates to the field of cosmetics, in particular to a composition with scalp care and hair loss prevention functions, application of the composition, a shampoo product and a preparation method of the shampoo product.

Background

At present, as the pressure of life is increased, the problems of scalp and hair loss are increased, a plurality of hair washing products are available on the market, but the hair washing products are mainly used for cleaning and caring hair, and a plurality of hair loss prevention hair washing products are available, but the hair is smooth, the pulling force is reduced during combing, so that the hair is less in hair loss, and the appearance phenomenon is all that is.

In order to prevent alopecia, the method of surgical treatment or drug treatment is also adopted, but the cost is high, the safety is poor, and the alopecia can be caused again if the nursing is inappropriately stimulated.

Disclosure of Invention

An object of embodiments of the present application is to provide a composition for scalp care and hair loss prevention, an application thereof, a shampoo product and a preparation method thereof.

The scalp, unlike the skin in other parts of the body, has a large number of large hair follicles, each with large sebaceous glands, which are capable of producing thick and long hair fibers. In addition, hair follicles are closely connected with the nerve network and the vascular network which are complicated. There are many causes of alopecia, firstly, the sebaceous glands of hair follicles can store considerable amounts of sebum, and in normal individuals, the sebaceous glands present a complex secretory rhythm, and the pores rich in sebum give lipophilic microorganisms and parasites, such as propionibacteria, staphylococcus epidermidis, malassezia and helminths, an aerobic environment, which can lead to the generation of scalp inflammation and also to alopecia; secondly, if blood circulation is not smooth, namely the hair follicle is not nutritional enough, the hair follicle can also cause alopecia; third, hair loss can also result when the scalp is inflamed.

In view of this, the application mainly carries out daily care and repair to the scalp through shampoo products from various target points of the alopecia mechanism in multiple dimensions, and the scalp is healthier, so that the purpose of preventing alopecia is achieved.

In a first aspect, the present application provides a composition for scalp care and hair loss prevention comprising, in mass percent:

1.0 to 35 percent of ginger juice, 0.01 to 2.0 percent of allantoin, 0.1 to 10.0 percent of panthenol, 0.1 to 5 percent of cacumen biotae extract, 0.005 to 0.1 percent of hydrolyzed chitin and 0.01 to 0.5 percent of salicylic acid; the balance being water.

The anti-hair loss composition for scalp care provided by the application realizes the synergistic effect by the synergistic cooperation of the components; the effects of the components are maximized, scalp is nursed from various paths of scalp hair follicles and scalp inflammations and multiple dimensions, so that the scalp is nourished and healthier, the optimal ideal state of anti-alopecia effect is achieved, and the anti-alopecia effect is finally achieved.

The hair care and hair loss prevention composition can be applied to a shampoo product, so that the shampoo product can achieve the hair care and hair loss prevention effect. Thereby being capable of replacing the conventional anti-alopecia chemical treatment medicaments and medical treatment means and showing the ideas of convenience, health and safety.

In other embodiments of the present application, the method of using the above composition with scalp care and hair loss prevention comprises: the composition with scalp care and hair loss prevention functions is directly compounded into a shampoo product for use, and is used for daily shampoo; is convenient, simple and quick.

In other embodiments of the present application, a composition having scalp care anti-hair loss comprises, in mass percent:

5 to 20 percent of ginger juice, 0.05 to 1.0 percent of allantoin, 0.2 to 5 percent of panthenol, 0.3 to 3 percent of cacumen biotae extract, 0.01 to 0.08 percent of hydrolyzed chitin and 0.05 to 0.3 percent of salicylic acid; the balance being water.

12% of ginger juice, 0.3% of allantoin, 1.0% of panthenol, 0.9% of cacumen biotae extract, 0.016% of hydrolyzed chitin, 0.1% of salicylic acid and the balance of water.

In other embodiments of the present application, the active ingredients in the ginger juice include: gingerols, paragingerols, gingerols, and gingerols.

In other embodiments of the present application, the active ingredients in the cacumen Platycladi extract include: hinokitiol, neocedar biflavone Huang, amentoflavone, quercetin, quercitrin, myricetin, and kaempferol.

In other embodiments of the present application, the anti-hair loss composition with scalp care is free of purslane extract.

In a second aspect, the present application provides the use of the composition for scalp care and hair loss prevention provided in the first aspect in a shampoo product.

In a third aspect, the present application provides a shampoo product comprising the scalp care anti-hair loss composition provided in the first aspect.

In other embodiments of the present application, a shampoo product comprises:

10 to 15 parts of a composition for scalp care and hair loss prevention;

10-18 parts of ammonium laureth sulfate;

1-5 parts of ammonium lauryl sulfate;

1-6 parts of cocamidopropyl betaine;

1-5 parts of polydimethylsiloxane;

0.8 to 1.5 parts of cocoamide MEA;

0.8-2.0 parts of glycol distearate;

0.1 to 0.4 part of guar hydroxypropyl trimethyl ammonium chloride;

0.1 to 0.5 part of sodium chloride;

0.05 to 0.2 part of EDTA disodium.

In a fourth aspect, the present application provides a method of preparing a shampoo product comprising:

adding uniformly dispersed guar hydroxypropyl trimethyl ammonium chloride, EDTA disodium and citric acid into a water bath kettle at 80-85 ℃;

adding ammonium laureth sulfate, ammonium laureth sulfate and cocamidopropyl betaine into the water bath kettle, and uniformly dissolving;

then adding cocoamide MEA and glycol distearate into the water bath kettle, and preserving heat for 15-25min;

then cooling to below 45 ℃, and adding polydimethylsiloxane, sodium chloride and the composition for scalp care and hair loss prevention into a water bath kettle; stirring until the mixture is dissolved uniformly.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate certain embodiments of the present application and therefore should not be considered limiting of the scope.

FIG. 1 shows a histogram of the results of a relative width test of the dorsal aortic blood vessel (DA) of a zebra fish embryo;

FIG. 2 shows a bar graph of the soothing efficacy test results;

FIG. 3 shows a bar graph of repair efficacy test results;

FIG. 4 shows a histogram of the relative expression levels of zebra fish 5α -reductase genes.

Detailed Description

For the purposes of making the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments.

Thus, the following detailed description of the embodiments of the present application is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application. The shampoo materials, reagents and the like used in the present application are materials and reagents commercially available from conventional markets and the like unless otherwise specified.

Embodiments of the present application provide a composition for scalp care and hair loss prevention, comprising, in mass percent:

Further, in some embodiments of the present application, the anti-hair loss composition with scalp care comprises, in mass percent:

Further, in some embodiments of the present application, the active ingredients in the ginger juice include: gingerols, paragingerols, gingerols, and gingerols.

In some embodiments of the present application, the ginger juice may be derived from ginger.

Ginger is fresh rhizome of ginger. Gingerol is an effective component in ginger, and mainly comprises gingerols, paragingerols, gingerols, and the like, and chemical components of gingerols comprise 3-methoxy-4-hydroxyphenyl functional groups. Ginger has anti-inflammatory and antifungal effects, and can be used for treating infections caused by Trichophyton mentagrophytes, pityrosporum ovale, etc. According to the scheme, ginger juice is compounded with other components and is used for seborrhea and dandruff, so that a synergistic effect is achieved, alopecia can be effectively prevented, and scalp itching is inhibited.

Further, in some embodiments of the present application, allantoin is an amphoteric compound, can combine a plurality of substances to form a double salt, has effects of light protection, sterilization, corrosion prevention, pain relief, and oxidation resistance, can keep skin moisture, moisturize and soften, is a specific additive for cosmetics such as cosmetology and hairdressing, and is widely used as an additive for freckle cream, acne liquid, shampoo, soap, toothpaste, shaving lotion, hair conditioner, astringent, antiperspirant deodorant, and the like. The cosmetic containing allantoin has effects in protecting tissue, hydrophilizing, absorbing water, and preventing water emission. The allantoin-added lipstick and facial cream can make skin and lips soft and elastic, and have beautiful luster. Allantoin promotes tissue growth, cellular metabolism, and softens the stratum corneum proteins. For example, the functions of maccare, moisturizing and exfoliating the lips are mainly the role of allantoin.

The allantoin is creatively compounded with other components and is used for shampoo products such as hair cream, hair cream and shampoo, the synergistic effect is achieved, the protection effect on hair can be achieved, and the hair can be prevented from being forked and continuously broken.

Further, in some embodiments of the present application, panthenol acts as a moisturizing agent, which increases hydration of the stratum corneum, reduces TEWL, improves skin roughness and relieves skin after irritation. In addition, it can activate fibroblast proliferation and accelerate epithelial cell regeneration, so it can help wound healing.

The proposal creatively compounds panthenol with other components, is used for washing and caring hair, realizes the synergistic effect, can well strengthen the toughness of hair and reduce the damage caused by mechanical process (such as hair combing); the moisture content of the hair is maintained, so that the hair is not dry and frizzy.

Further, in some embodiments of the present application, the active ingredients in the cacumen Platycladi extract include: hinokitiol, neocedar biflavone Huang, amentoflavone, quercetin, quercitrin, myricetin, and kaempferol.

Further, in some embodiments of the present application, the main components of the cacumen Platycladi are tannins and flavonoids containing hinokitiol, neocedar bis Huang, amentoflavone, quercetin, myricetin, kaempferol, etc., wherein the content of quercetin is highest, the mesoporosity tannin is tannic acid, the leaves of the cacumen Platycladi also contain the volatile oil component of the carpenter, the highest content is 2, 2-tetramethyl-3, 7-cyclodecadin-1-methanol and cedrol, and further contain cypress brain, thujaone, a-pinene, β -pinene, pine acetate grease, isopsoralene, α -caryophyllene, etc.

Further, in some embodiments of the present application, the extract of biota orientalis has an inhibitory effect on staphylococcus aureus, escherichia coli, catarrhal coccus, streptococcus a, pneumococcus, bacillus dysenteriae, typhoid bacillus and the like, and also has an inhibitory effect on the growth of bacillus tuberculosis, and phenolic substances in the extract of biota orientalis have an effect of resisting human pathogenic fungi, so that the extract of biota orientalis can better inhibit the activity of candida. The arborvitae extract has anti-inflammatory effect on inhibition test of toe edema of rats, inhibition of urocanic acid enzyme and the like.

The arborvitae extract is creatively compounded with other components and used for washing and caring hair products, the synergistic effect is achieved, and the hair follicle and scalp inflammation can be improved.

Further, in some embodiments of the present application, the hydrolyzed chitin is a natural organic matter that can be completely biodegradable, is a deacetylated product of chitin, has a certain antibacterial effect, is a relatively ideal antibacterial raw material, and is particularly excellent in inhibition ability to malassezia, and meanwhile, the aqueous solution is cationic, has high affinity to hair and scalp, has a certain conditioning effect, and has no accumulation effect.

The proposal creatively compounds the hydrolyzed chitin with other components, is used for washing and caring hair products, realizes the synergistic effect and can improve the inflammation of hair follicles and scalp.

Further, in some embodiments of the present application, salicylic acid can solubilize the intercellular substances of the horny layer, thereby allowing the horny layer to be exfoliated, so that the accumulated horny layer can be removed, metabolism can be promoted, and cell viability is enhanced if the epidermal cells are all fresh and full of living tender cells. Salicylic acid is fat-soluble, can permeate deep layer of pores along sebaceous glands secreting grease, is favorable for dissolving old and piled horny layer in pores, and improves blocking situation of pores, so that acne can be blocked, and enlarged pores can be reduced. Salicylic acid acts on hair follicle wall cells, can help to clear blocked hair follicle, correct abnormal cell shedding, can prevent pore blocking for slight acne, is most effective for blackhead acne, can reduce abnormal hair follicle wall shedding phenomenon, and can prevent new focus generation.

The salicylic acid and other components are creatively compounded in the scheme of the application, and the salicylic acid and other components are used for washing and caring hair products, so that the synergistic effect is realized, hair follicles can be improved, hair follicle growth is promoted, and hair follicle shedding is reduced.

Further, illustratively, in some embodiments of the present application, having a scalp care anti-hair loss composition comprises, in mass percent:

5%, 6%, 8%, 10%, 15%, 18%, 20% ginger juice or a range between any two of the foregoing values;

allantoin 0.05%, 0.06%, 0.08%, 0.1%, 0.5%, 0.6%, 0.8%, 1.0%, or a range between any two of the foregoing;

panthenol 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5% or a range between any two of the foregoing values;

0.3%, 0.5%, 0.8%, 1%, 1.5%, 1.8%, 2%, 2.5%, 2.8%, 3% of cacumen Platycladi extract or a range between any two of the foregoing values;

0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08% or a range between any two of the foregoing;

salicylic acid 0.05%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.26%, 0.28%, 0.3% or a range between any two of the foregoing;

the balance being water.

Further, in some embodiments of the present application, the anti-hair loss composition with scalp care is free of purslane extract.

In some embodiments of the present application, the method for preparing the composition for scalp care and hair loss prevention includes: the components are mixed and stirred uniformly, namely the hair loss prevention composition for scalp care.

Further, in some embodiments of the present application, there is provided the use of any one of the embodiments provided above in a shampoo product with a scalp care anti-hair loss composition.

Some embodiments of the present application provide a shampoo product comprising the hair loss prevention composition with scalp care provided by any one of the preceding embodiments.

Further, in some embodiments of the present application, a shampoo product comprises:

10 to 15 parts of a composition for scalp care and hair loss prevention;

10-18 parts of ammonium laureth sulfate;

1-5 parts of ammonium lauryl sulfate;

1-6 parts of cocamidopropyl betaine;

1-5 parts of polydimethylsiloxane;

0.8 to 1.5 parts of cocoamide MEA;

0.8-2.0 parts of glycol distearate;

0.1 to 0.4 part of guar hydroxypropyl trimethyl ammonium chloride;

0.1 to 0.5 part of sodium chloride;

0.05 to 0.2 part of EDTA disodium.

Illustratively, in some embodiments of the present application, a shampoo product comprises:

10. 11, 12, 13, 14 or 15 parts of a hair loss preventing composition having scalp care;

10. 11, 12, 13, 14, 15, 16, 17 or 18 parts of ammonium laureth sulfate;

1.2, 3, 4 or 5 parts of ammonium lauryl sulfate;

1.2, 3, 4, 5 or 6 parts of cocamidopropyl betaine;

1.2, 3, 4 or 5 parts of polydimethylsiloxane;

0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 parts cocoamide MEA;

0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.6, 1.8, or 2.0 parts ethylene glycol distearate;

0.1, 0.2, 0.3, or 0.4 parts guar hydroxypropyl trimonium chloride;

0.1, 0.2, 0.3, 0.4 or 0.5 parts of sodium chloride;

0.05, 0.06, 0.08, 0.1, 0.12, 0.15, 0.18 or 0.2 parts disodium EDTA.

In other optional embodiments of the present application, the shampoo product further includes: 0.08 to 0.1 weight portion of preservative, 0.01 to 0.05 weight portion of citric acid and 0.2 to 0.5 weight portion of essence; and the balance water (make up to 100 parts by weight with water).

Some embodiments of the present application provide a method for preparing a shampoo product, comprising:

Further, in some embodiments of the present application, a method of preparing a shampoo product comprises the steps of:

step S1, adding uniformly dispersed guar hydroxypropyl trimethyl ammonium chloride, EDTA disodium and citric acid into a water bath kettle at 80-85 ℃.

Further, in some embodiments of the present application, uniformly dispersed guar hydroxypropyl trimethylammonium chloride, disodium EDTA, citric acid are added to a water bath at 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, or 85 ℃.

Illustratively, in some embodiments of the present application, water is heated to 80-85 ℃ in a water bath, then the water is kept constant, and then guar hydroxypropyl trimonium chloride, disodium EDTA, citric acid, which are pre-dispersed with water, are added.

And S2, adding ammonium laureth sulfate, ammonium laurylsulfate and cocamidopropyl betaine into the water bath kettle obtained in the step S1, and uniformly dissolving.

Illustratively, in some embodiments of the present application, ammonium laureth sulfate, ammonium laurylsulfate, cocamidopropyl betaine are added to the water bath obtained in step S1 to completely dissolve.

And step S3, adding cocoamide MEA and glycol distearate into the water bath kettle obtained in the step S2, and preserving heat for 15-25min.

Illustratively, in some embodiments of the present application, cocamide MEA, ethylene glycol distearate, and/or other additives are added to the water bath obtained in step S2 for 15min, 16min, 17min, 18min, 19min, 20min, 21min, 22min, 23min, 24min, or 25min.

Step S4, cooling the water bath kettle obtained in the step S3 to below 45 ℃, and adding polydimethylsiloxane, sodium chloride and the scalp care anti-hair loss composition into the water bath kettle; stirring until the mixture is dissolved uniformly.

Illustratively, in some embodiments of the present application, the water bath obtained in step S3 is cooled to below 45 ℃, the rest of the polydimethylsiloxane, sodium chloride, preservative and essence are added, and finally the scalp care anti-hair loss composition is added, and stirring is continued for 15 minutes.

The application adds an anti-hair loss composition for scalp care into a shampoo product. The hair loss prevention composition for scalp care of the invention has the advantages that scalp is healthier by caring scalp, so that the effects of preventing hair loss or reducing hair loss are achieved.

The following examples are set forth to better illustrate the present application.

Examples

Provided is a composition for scalp care and hair loss prevention, the specific composition of which is shown in Table 1.

TABLE 1

Comparative example

A composition is provided with specific compositions as shown in table 2.

TABLE 2

The properties of the compositions provided by each example or each comparative example were tested.

Experimental example

The compositions provided in examples 1 to 7 and comparative examples 1 to 7 were tested in four dimensions of repair promotion rate, neutrophil aggregation inhibition rate, 5. Alpha. Reductase gene expression inhibition rate, and vasodilation rate using a zebra fish test model.

1. Zebra fish embryo tail fin repair promotion test: the test was performed using the zebra fish embryo tail fin excision model. The 24 tail fin cut fish embryos are exposed to 0.01g/L test sample solution, a blank control group, a positive control group and a model control group are simultaneously arranged, the fish embryos are photographed under a microscope after 48 hours of exposure, and the tail fin regeneration length is measured and is subjected to statistical analysis.

Wherein: s-average value of tail fin length of fish embryo treated by test substance;

average value of tail fin length of fish embryo of M-model control group;

average value of tail fin length of fish embryo in B-blank control group.

The mean and standard error of each test group were calculated and the statistical treatment results were expressed as mean ± standard error. And performing variance analysis on the data by using statistical software, and performing double-tail T test on the tail fin length between the tested object group and the model control group to obtain a p value. p <0.05 indicates a significant difference.

The higher the repair promoting rate, the better the repair promoting effect of the composition.

2. Zebra fish 5α -reductase gene expression test: 3 groups of 36-tail 5-day large zebra fish are exposed to 0.05g/L test sample solution, a blank control group is arranged at the same time, RNA extraction, cDNA synthesis and real-time PCR amplification are carried out on the zebra fish after 24 hours of exposure, beta-actin is taken as a housekeeping gene, ct is taken as an amplification result, and the relative expression quantity of 5 alpha reductase genes is calculated and is subjected to statistical analysis.

Wherein: 2 ^-ΔΔCt Relative expression level of genes.

The mean and standard error of each test group were calculated and the statistical treatment results were expressed as mean ± standard error. And performing analysis of variance on the data by using statistical software, and performing double-tail T test on the relative expression quantity of the target genes between the tested object group and the blank control group to obtain a p value. p <0.05 indicates a significant difference.

The larger the 5 alpha reductase gene expression inhibition value is, the better the androgen inhibition performance of the composition is.

3. Zebra fish embryo neutrophil aggregation inhibition assay: and (3) testing by using a model of neutrophil aggregation caused by copper sulfate-induced nerve dome cell injury in the embryonic side line region of the zebra fish. 24 fish embryos are exposed to 0.16mg/L copper sulfate pentahydrate and 0.05g/L test sample solution, a blank control group, a positive control group and a model control group are simultaneously arranged, the fish embryos are fixed and sudan black stained after 40min exposure, and the number of neutrophils in a lateral line area is counted and subjected to statistical analysis.

Wherein:

s-average number of neutrophils in the group fish embryos treated with the test substance in units of pellet per tail (pellet/tail);

average number of neutrophils in the fish embryo of the M-model control group in units of granulocyte/tail (granulocyte/tail);

average number of neutrophils in fish embryos of B-blank in units of pellet per tail (pellet/tail).

The mean and standard error of each test group were calculated and the statistical treatment results were expressed as mean ± standard error. And performing analysis of variance on the data by using statistical software, and performing double-tail T test on the number of neutrophils between the tested object group and the model control group to obtain a p value. p <0.05 indicates a significant difference.

The higher the neutrophil aggregation rejection rate, the better the soothing properties of the composition.

4. Zebra fish embryo vasodilation efficacy test: 12-tail 3-day-old Fli1 EGFP transgenic zebra fish embryos are exposed to 0.01g/L test sample solution, a blank control group is arranged at the same time, and after exposure for 3 hours, each endpoint is photographed under a microscope for width measurement, average value is obtained and statistical analysis is carried out. The tail zebra fish embryo is the dorsal aortic vessel. The 5 non-width measurements of the dorsal aortic blood vessels of each fish embryo were then averaged and statistically analyzed using software.

Wherein:

s-average value of the width of blood vessels of dorsal aorta of the fish embryo treated by the test substance;

b-average value of the width of the dorsal aortic blood vessels of fish embryos of the blank group;

the mean and standard error of each test group were calculated and the statistical treatment results were expressed as mean ± standard error. And performing analysis of variance on the data by using statistical software, and performing double-tail T test on the width of the back aorta blood vessel between the test object group and the blank control group to obtain a p value. p <0.05 indicates a significant difference.

The higher the vasodilation rate, the better the vasodilation performance of the composition.

The test results of the zebra fish in 4 dimensions are shown in Table 3.

TABLE 3 Table 3

The hair-loss prevention effect is evaluated by comprehensively evaluating the scalp care condition through the test results of the zebra fish in 4 dimensions.

As is clear from the test in Table 3, examples 1 to 7 have a certain promotion effect in four dimensions of the maintenance promotion rate, the neutrophil aggregation inhibition rate, the 5. Alpha. Reductase gene expression inhibition rate and the vasodilation rate. Therefore, the scalp can be repaired from multiple dimensions by the aid of the scheme, the scalp is more nutritious, the scalp is healthier, the optimal ideal state of the alopecia preventing effect is achieved, and the alopecia preventing effect is finally achieved.

The comparison with the test results of comparative examples 1 to 7 shows that the components of the examples act synergistically to repair the scalp from multiple dimensions, so that the scalp is more nutritious and healthier, the optimal ideal state of the alopecia preventing effect is achieved, and the alopecia preventing effect is finally achieved.

Compared with example 4, the values of four dimensions of the repair promotion rate, the neutrophil aggregation rejection rate, the 5 alpha reductase gene expression inhibition rate and the vasodilation rate of each comparative example are all significantly lower than those of example 4; it can be seen that the anti-hair loss effect of the comparative example is significantly lower than that of the examples. Therefore, the components of the composition are synergistically matched, a synergistic effect is generated, the scalp is repaired from multiple dimensions, the scalp is nourished, the scalp is healthier, the optimal ideal state of the alopecia preventing effect is achieved, and the alopecia preventing effect is finally achieved. Further, comparison of comparative example 7 with example 4 of the present application shows that when other ingredients, such as purslane extract, etc., are added to the composition of the present application, the effect of the composition of the present application is rather inhibited.

Thus, the synergistic combination of the individual components of the compositions of the present application does not allow for the optional addition or reduction of components that might otherwise bring about inhibition.

[ application example ]

The composition provided in example 4 was applied to a shampoo product as a sample treatment group to examine the hair loss preventing effect of the shampoo product.

The shampoo formulations of the compositions provided in example 4 are shown in table 4 below.

TABLE 4 Table 4

Water and its preparation method	To 100g
		Ammonium laureth sulfate	11.0g
Ammonium lauryl sulfate	8.0g
		Cocamidopropyl betaine	5.0g
Polydimethylsiloxane	4.0g
		Cocoamide MEA	1.2g
Ethylene glycol distearate	1.5g
		Guar hydroxypropyl trimethyl ammonium chloride	0.3g
Sodium chloride	0.3g
		EDTA disodium salt	0.1g
Preservative agent	0.1g
		Citric acid	0.05g
Essence	0.5g
		Example 4 provides a composition	14.516g

A shampoo formulation without the above-described hair loss prevention composition with scalp care was used as a blank comparative example. The specific composition is shown in Table 5.

TABLE 5

Water and its preparation method	To 100g
		Ammonium laureth sulfate	11.0g
Ammonium lauryl sulfate	8.0g
		Cocamidopropyl betaine	5.0g
Polydimethylsiloxane	4.0g
		Cocoamide MEA	1.2g
Ethylene glycol distearate	1.5g
		Guar hydroxypropyl trimethyl ammonium chloride	0.3g
Sodium chloride	0.3g
		EDTA disodium salt	0.1g
Preservative agent	0.1g
		Citric acid	0.05g
Essence	0.5g

The preparation method of the shampoo product comprises the following steps:

heating water to 80-85 ℃ in a water bath kettle, keeping the temperature, adding guar hydroxypropyl trimethyl ammonium chloride, EDTA disodium and citric acid which are dispersed in water in advance, adding laureth ammonium sulfate, laureth ammonium sulfate and cocamidopropyl betaine, adding cocamide MEA and ethylene glycol distearate after the mixture is completely dissolved, keeping the temperature for 20 minutes, cooling to below 45 ℃, adding the rest polydimethylsiloxane, sodium chloride, preservative and essence, finally adding the composition of the example 4 (the application comparative example is not added), and continuously stirring for 15 minutes.

And detecting the performance of the shampoo product.

1. The test anti-hair loss effect was scored by the evaluation method by testing 20 volunteers (10 persons per formula, age 25-60 years, with dispersibility, non-health related hair loss and thin, no air hair) using the samples of example 4 and the blank comparative example, respectively, and the final results were averaged. The shampoo is used for shampoo twice a week for 12 weeks, and the anti-hair loss effects before and after 12 weeks of use are respectively compared, and the follow-up test is carried out to examine the hair loss improvement condition of each volunteer.

Test scoring criteria: the scores were rated as five grades 1, 2, 3, 4 and 5, wherein 1 is that the hair loss situation is not improved at all, 2 is that the hair loss situation is not improved obviously, 3 is that the hair loss situation is slightly improved, 4 is that the hair loss situation is improved well, 5 is that the hair loss situation is improved obviously, the improvement situation is evaluated at intervals of 0.1, and finally the score average value of 10 persons in each group is integrated after trial for 12 weeks, and the calculation results are shown in the test score results of table 6.

Table 6 formulation and test results of shampoo provided in example 4 and blank comparative example

As is clear from table 6, the scalp care anti-hair loss composition of example 4 can achieve the same effect in shampoo, so that the scalp is healthier, the improvement of the hair loss condition is ideal, and the anti-hair loss effect is finally achieved.

While the anti-hair loss effect of the blank comparative example was significantly lower than that of the example 4 regimen.

2. Example 4 sample treatment group and blank comparative example were tested by zebra fish multi-dimension.

(1) Relative width test of zebra fish embryo dorsal aortic vessel (DA): 12-tail 3-day-old Fli1 EGFP transgenic zebra fish embryos are exposed to 0.01g/L test sample solution, a blank control group is arranged at the same time, and after exposure for 3 hours, each endpoint is photographed under a microscope for width measurement, average value is obtained and statistical analysis is carried out. The tail zebra fish embryo is the dorsal aortic vessel. The 5 non-line width measurements of the dorsal aortic blood vessels of each fish embryo were then averaged and statistically analyzed using software.

The test results are shown in figure 1 of the specification.

As can be seen from fig. 1: the vasodilation rate of the sample treatment group reaches 14 percent and p is less than 0.05, which shows that the blood vessel of the zebra fish embryo aorta can be obviously dilated, and the sample group has the effect of dilating the blood vessel.

(2) Test of soothing efficacy: characterization by neutrophil number: zebra fish embryo neutrophil aggregation inhibition assay: and (3) testing by using a model of neutrophil aggregation caused by copper sulfate-induced nerve dome cell injury in the embryonic side line region of the zebra fish. 24 fish embryos are exposed to 0.16mg/L copper sulfate pentahydrate and 0.05g/L test sample solution, a blank control group, a positive control group and a model control group are simultaneously arranged, the fish embryos are fixed and sudan black stained after 40min exposure, and the number of neutrophils in a lateral line area is counted and subjected to statistical analysis.

The test results are shown in figure 2 of the specification.

As can be seen from fig. 2: the aggregation inhibition rate of the sample treatment group is reduced by 23% compared with that of a blank control group, and p is less than 0.05, and the sample treatment group can obviously inhibit the aggregation of the zebra fish embryo neutrophils, so that the sample group has a relieving effect.

(3) And (3) repairing efficacy test: characterization of zebra fish tail fin relative length: the test was performed using the zebra fish embryo tail fin excision model. The 24 tail fin cut fish embryos are exposed to 0.01g/L test sample solution, a blank control group, a positive control group and a model control group are simultaneously arranged, the fish embryos are photographed under a microscope after 48 hours of exposure, and the tail fin regeneration length is measured and is subjected to statistical analysis.

The test results are shown in figure 3 of the specification.

As can be seen from fig. 3: the repair promotion rate of the zebra fish embryo tail fin of the sample treatment group is 30%, and p is less than 0.05, and the sample treatment group can obviously promote the regeneration of the zebra fish embryo tail fin, so that the sample has repair performance.

(4) Zebra fish 5α -reductase gene expression test: 3 groups of 36-tail 5-day large zebra fish are exposed to 0.05g/L test sample solution, a blank control group is arranged at the same time, RNA extraction, cDNA synthesis and real-time PCR amplification are carried out on the zebra fish after 24 hours of exposure, beta-actin is taken as a housekeeping gene, ct is taken as an amplification result, and the relative expression quantity of 5 alpha-reductase genes is calculated and is subjected to statistical analysis.

The test results are shown in figure 4 of the specification.

As can be seen from fig. 4: the lower gene expression level of the sample treatment groups in srd a1 and srd a2b shows that the inhibition rate of the expression of the zebra fish 5 alpha-reductase I type gene (srd 5a 1) and the inhibition rate of the expression of the zebra fish 5 alpha-reductase II type gene (srd 5a2 b) are higher than those of the blank control group, and the sample has the performance of inhibiting the androgen to relieve the emasculation.

In summary, the performances of the sample in example 4 of the present application are better than those of the blank control group, so that it can be demonstrated that the anti-hair loss composition for scalp care of the present application can achieve the same effect as the composition when applied in shampoo, so that the scalp is healthier, the hair loss situation is improved ideally, and the anti-hair loss effect is finally achieved.

The embodiments described above are some, but not all, of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application.

Claims

1. A composition for preventing hair loss with scalp care, characterized in that the composition for preventing hair loss with scalp care comprises, in mass percent:

2. The composition for scalp care and hair loss prevention according to claim 1, wherein the composition for scalp care and hair loss prevention comprises, in mass percent:

3. The composition for scalp care and hair loss prevention according to claim 1, wherein the composition for scalp care and hair loss prevention comprises, in mass percent:

4. A composition having scalp care and hair loss prevention as claimed in any one of claims 1 to 3,

the effective components in the ginger juice comprise: gingerols, paragingerols, gingerols, and gingerols.

5. A composition having scalp care and hair loss prevention as claimed in any one of claims 1 to 3,

the active ingredients in the biota orientalis leaf extract comprise: hinokitiol, neocedar biflavone Huang, amentoflavone, quercetin, quercitrin, myricetin, and kaempferol.

6. A composition having scalp care and hair loss prevention as claimed in any one of claims 1 to 3,

the composition for scalp care and hair loss prevention does not contain purslane extract.

7. Use of a composition having scalp care and hair loss prevention as claimed in any one of claims 1 to 6 in a shampoo product.

8. A shampoo product comprising a composition having scalp care and hair loss prevention as claimed in any one of claims 1 to 6.

9. The shampoo product according to claim 8, wherein the hair conditioning composition comprises, in parts by weight,

the shampoo product comprises:

10-15 parts of the composition for scalp care and hair loss prevention;

10-18 parts of ammonium laureth sulfate;

1-5 parts of ammonium lauryl sulfate;

1-6 parts of cocamidopropyl betaine;

1-5 parts of polydimethylsiloxane;

0.8 to 1.5 parts of cocoamide MEA;

0.8-2.0 parts of glycol distearate;

0.1 to 0.4 part of guar hydroxypropyl trimethyl ammonium chloride;

0.1 to 0.5 part of sodium chloride;

0.05 to 0.2 part of EDTA disodium.

10. A method of preparing a shampoo product according to claim 9, comprising:

then cooling to below 45 ℃, and adding polydimethylsiloxane, sodium chloride and the scalp care anti-hair loss composition into the water bath kettle; stirring until the mixture is dissolved uniformly.