CN117694549A - Moisturizing, freckle-removing and whitening composition, preparation method and application - Google Patents
Moisturizing, freckle-removing and whitening composition, preparation method and application Download PDFInfo
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- CN117694549A CN117694549A CN202410161698.9A CN202410161698A CN117694549A CN 117694549 A CN117694549 A CN 117694549A CN 202410161698 A CN202410161698 A CN 202410161698A CN 117694549 A CN117694549 A CN 117694549A
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Abstract
The invention belongs to the technical field of functional foods, and particularly relates to a moisturizing, freckle-removing and whitening composition, a preparation method and application. The raw materials of the composition comprise: elastin peptide, tremella, aronia melanocarpa fruit powder, guan Shanying pollen and lactobacillus plantarum S58. The preparation method comprises pulping Tremella to obtain pulp; mixing and extracting Aronia melanocarpa fruit powder and flos Rosae Laevigatae powder to obtain extractive solution; mixing the extract with the beating solution to obtain a solution, adding lactobacillus plantarum S58, and mixing to obtain a mixed solution; finally, the elastin peptide and the mixed solution are mixed or added with the flavoring agent for mixing, thus obtaining the medicine. The composition prepared by the invention has the effects of enhancing skin metabolism, regulating and treating chloasma on the whole, promoting collagen regeneration, preserving moisture and improving skin elasticity.
Description
Technical Field
The invention belongs to the technical field of foods, and particularly relates to a moisturizing, freckle-removing and whitening composition, a preparation method and application thereof.
Background
Fat and muscle are the greatest supporting force of skin, and subcutaneous fat loss, muscle relaxation, relaxation sagging, stain deposition and skin aging are caused by various reasons such as human aging, weight loss, malnutrition, lack of exercise and the like.
The most important cause of skin relaxation is due to loss of elasticity of dermal tissue of the skin. Collagen and elastic tissue fibers exist in dermis, which changes with age, and reduces texture and content.
The existing methods for improving skin relaxation mainly comprise the following steps: supplementing collagen, but as for collagen, which is decomposed in the gastrointestinal tract as a macromolecule, whether collagen can be synthesized again in vivo has been controversial, and its improving effect is not obvious; secondly, cosmetic care is employed to stimulate cellular metabolism and blood circulation. The method has certain irritation to organism, and has obvious short-term effect, but is not suitable for long-term use.
Chloasma is a common acquired hyperpigmentation skin disease occurring in the face, and recently, it has been found that the occurrence of this disease is related to microecological balance, metabolic abnormality and poor quality cosmetics. At present, the treatment of chloasma mainly comprises sun protection, comprehensive treatment of oral and external medicines and laser treatment is assisted. The medicine treatment mainly comprises 3% hydroquinone cream and 0.05% -0.1% tretinoin cream which are used for external application alternately or in a mixed mode; vitamin C and some traditional Chinese medicines are orally taken, and in addition, 20% -70% of glycollic acid is commonly used for treatment of fruit acid. In addition, the laser surgery treatment is suitable for patients with refractory chloasma, such as YAG laser and Q-switch ruby laser, and has a certain clinical curative effect, but needs to be vigilant for inflammation, pigment increase or scar formation, and even keloids appear.
In addition, the strong pulse light treatment is beneficial to controlling inflammatory response of skin lesions, has a certain effect on some chloasma, but has more adverse reactions.
The dietotherapy has high safety coefficient, can be taken for a long time and has reasonable compatibility. Can regulate the unbalance of the organism on the whole and is more and more favored in the aspects of improving the skin and the skin tightness, improving chloasma and the like.
Chinese patent application CN104146266a discloses a total nutrient formula food for patients with chloasma, a specific total nutrient formula food or a non-total nutrient formula food. The formula is developed according to the requirements of general rule of formula food for special medical use and the constitution characteristics of chloasma patients and the essence of traditional Chinese medicine for eliminating the middle warmer, tonifying and balancing.
Another Chinese patent application CN108813637a discloses a glutathione oral liquid and a preparation method thereof, which comprises the following raw materials in parts by weight: 15-30 parts of yeast extract, 10-30 parts of collagen, 5-10 parts of stachyose and vitamin C
0.3-0.6 part, nicotinic acid 0.01-0.03 part, water 40-50 parts and sucralose 0.05-0.15 part, wherein the mass fraction of glutathione in the yeast extract is 10-90%. The oral liquid is prepared by adopting an isotonic isobaric technology, the osmotic pressure of the oral liquid is similar to that of a human body, the permeable membrane absorption can be realized, the possibility of denaturation in the digestion process is avoided, the absorption rate of the human body to glutathione is improved, and the oral liquid is easier to be absorbed by the human body.
The Chinese further patent application CN104286288A discloses a chamomile violet toxin-expelling and freckle-removing tea which is mainly prepared from the following raw materials: 5-10 g of chamomile dried product, 5-10 g of violet dried product, 3-6 g of cassia seed, 10-15 g of medlar, 5-10 g of liquorice, 10-15 g of agilawood, 5-15 g of honeysuckle and 10-20 g of rock candy. Has the effects of whitening skin, removing freckle, promoting skin metabolism, promoting endocrine circulation, delaying cell aging, improving immunity, expelling toxin and caring skin.
The prior art has explored the special medical formula of chloasma, dietary therapy of health products and treatment of traditional Chinese medicines, but the traditional Chinese medicine formula has a plurality of raw materials, which is unfavorable for elucidation of the drug effect substance basis, but the whole regulation of the organism by pure foods, whitening peptide molecules and vitamin C is weaker, so that the problems of skin relaxation and chloasma can not be fundamentally improved.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides a moisturizing, freckle-removing and whitening composition, a preparation method and application. The composition prepared by the invention has the effects of enhancing skin metabolism, regulating and treating chloasma on the whole, promoting collagen regeneration, preserving moisture and improving skin elasticity.
In order to achieve the purpose of the invention, the technical scheme adopted is as follows:
a composition for moisturizing, removing freckles and whitening skin comprises the following raw materials: elastin peptide, tremella, aronia melanocarpa fruit powder, guan Shanying pollen and lactobacillus plantarum S58.
Preferably, the raw materials of the composition comprise the following components in parts by weight: 20-50 parts of elastin peptide, 1-50 parts of tremella, 0.1-5 parts of Aronia melanocarpa fruit powder, 1-10 parts of Guan Shanying pollen and 0.1-5 parts of lactobacillus plantarum S58.
Further preferably, the raw materials of the composition comprise the following components in parts by weight: 30-40 parts of elastin peptide, 10-20 parts of tremella, 0.5-2 parts of Aronia melanocarpa fruit powder, 5-10 parts of Guan Shanying pollen and 0.5-2 parts of lactobacillus plantarum S58.
Preferably, the lactobacillus plantarum S58 is an inactivated lactobacillus, and the concentration of the strain before inactivation is 4000 hundred million CFU/g.
Preferably, the composition further comprises a flavouring agent; the correctant is one or two selected from citric acid, sucralose and mogroside.
It is still another object of the present invention to provide a method for preparing the above composition, comprising the steps of:
(1) Pulping tremella to obtain pulp;
(2) Mixing and extracting Aronia melanocarpa fruit powder and flos Rosae Laevigatae powder to obtain extractive solution;
(3) Mixing the extract with the beating solution to obtain a solution, then adding lactobacillus plantarum S58, and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution or adding correctant and mixing.
Preferably, the beating in the step (1) is carried out by adding 3-5 times of water and beating for 3-5min.
Preferably, the extraction in the step (2) is a heating reflux extraction, and the extraction time is 1-2h.
Preferably, the step (2) is carried out by performing enzymolysis, filtering to obtain enzymolysis liquid and residue, adding aqueous solution containing 0.5-1% lecithin into residue, extracting to obtain feed liquid, and adding into enzymolysis liquid to obtain extract.
Preferably, the enzyme adopted by the enzymolysis comprises laccase, 1398 neutral protease and soybean peptide extracting enzyme in a mass ratio of 1:0.5-1:0.5-1; the enzymolysis pH is 5-7, the enzymolysis temperature is 30-50 ℃, and the enzymolysis time is 1-3h.
It is still another object of the present invention to provide the use of the above composition or the composition prepared by the preparation method for preparing a product for promoting collagen regeneration, moisturizing, improving skin elasticity and/or improving skin chloasma.
The elastin peptide has the functions of moisturizing and whitening skin, avoiding outflow of water and keeping the skin moist and tender; the skin color-changing agent has a plurality of hydroxyl groups, so that the skin color-changing agent not only can improve the skin color, but also has the effects of removing yellow and whitening; secondly, senile plaques can be desalted, and the peptide can reasonably inhibit the activity of tryptophan enzymes, so that the pigment is reduced; in addition, the skin-care product can also prevent aging, repair broken and embrittled elastic fiber net, repair the ductility of the skin, and enable the skin to be more ductile due to the softness and the flushing. The collagen peptide can enter the dermis layer of the skin immediately after being supplemented, and the skin chemical fiber tissue is recombined to promote basic metabolism of somatic cells.
Tremella, the fruiting body of fungus of tremella, the nutrient composition of tremella is quite abundant, contain protein, fat and multiple amino acid, mineral matter and polysaccharide in tremella; most of nutrients in the tremella after pulping are dissolved out, particularly the dissolution amount of tremella polysaccharide is greatly increased, the activity is greatly enhanced, and the tremella fuciformis has good effects of strengthening essence, tonifying kidney, tonifying qi, strengthening heart, beautifying, tendering skin and prolonging life.
Aronia melanocarpa (academic name: aronia melanocarpa (Michx.) Elliott) is a shrub of Aronia genus of Rosaceae, and its fruit and extract have special therapeutic effects on cardiovascular and cerebrovascular diseases such as heart disease and hypertension, and can be widely used in medicine and functional food industry in European and American regions. Anthocyanin, flavone (the content of fresh fruits is up to 0.25% -0.35%), polyphenol, multiple vitamins and mineral elements in the fruits. Polyphenols are very important substances that improve the structure and function of capillaries and blood vessels, and also contribute to stimulation and improvement of the circulatory system. Anthocyanin and flavone can maintain heart and organism health of human.
Guan Shanying pollen is a flower of Prunus Guan Shanying (Cerasus serrulate 'Sekiyama') of Rosaceae Li Yake. The variety of sakura is numerous and can reach hundreds. Of all sakura varieties, only sakura in Guanshan China is currently available as a new food raw material. Toxicity studies have shown to be safe. The total flavone content of the Guanshan cherry dried product is up to 11.25%, and the main components are sakura glycoside and sakura extract, so that the effect of removing nitrite is good. Other components include polysaccharide, organic acid, natural vitamin A, vitamin B, vitamin E, etc.
Lactobacillus plantarum S58 is separated from Chongqing pickle, and researches show that the probiotics can improve the occurrence and development of obesity and play a role in relieving obesity.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention adopts compatibility, adopts lactobacillus plantarum S58 to ferment the extracting solution, and the active components and the metabolites of the tremella, the Aronia melanocarpa fruit powder and the sakura powder plant extracts are obtained to synergistically improve the chloasma problem locally appearing on the skin.
(2) The invention has the advantages of fine formula and definite efficacy, and the elastin peptide can improve skin elasticity, activate fibroblasts and promote regeneration of collagen; the tremella serum, the black fruit Aronia melanocarpa fruit powder and the cherokee rose flower powder are subjected to enzymolysis to generate a large amount of polysaccharide, micromolecular multi-protein peptide and the like, which can activate fibroblasts and promote regeneration of elastin peptide and collagen; the components cooperate to moisturize and improve skin elasticity, and human body test feeding experiments prove that the components cooperate to promote skin metabolism, promote collagen regeneration, improve the color of facial chloasma and reduce the area of chloasma.
Detailed Description
The invention is further described in connection with the following detailed description. The following raw materials were commercially available conventional raw materials. Wherein laccase, 1398 neutral protease and soybean peptide extraction enzyme are available from Nanning Donghenghua biological technology Limited liability company; the tremella are dry tremella; lactobacillus plantarum S58 is supplied by Puenkang Biotechnology (Suzhou) Co., ltd, and is inactivated Lactobacillus plantarum S58, and the strain concentration before inactivation is 4000 hundred million CFU/g.
Example 1
The moisturizing and freckle-removing composition comprises the following raw materials in parts by weight: 30 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 8 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Example 2
The moisturizing and freckle-removing composition comprises the following raw materials in parts by weight: 40 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 5 parts of Guan Shanying pollen and 0.5 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 5 times of water into tremella, pulping for 5min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:0.5:0.5) by stirring, mixing, performing enzymolysis at 50deg.C and pH7.0 for 1 hr, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of water solution containing 0.5% lecithin into residue, reflux extracting for 1 hr, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.04:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Example 3
The moisturizing and freckle-removing composition comprises the following raw materials in parts by weight: 20 parts of elastin peptide, 15 parts of tremella, 0.5 part of Aronia melanocarpa fruit powder, 10 parts of Guan Shanying pollen and 2 parts of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 30deg.C and pH7.0 for 3h, boiling for 2min for inactivation, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.06:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Example 4
The moisturizing and freckle-removing composition comprises the following raw materials in parts by weight: 50 parts of elastin peptide, 5 parts of tremella, 0.1 part of Aronia melanocarpa fruit powder, guan Shanying pollen 3 and 5 parts of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 5min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1), stirring and mixing, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 0.5% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Example 5
The moisturizing and freckle-removing composition comprises the following raw materials in parts by weight: 20 parts of elastin peptide, 50 parts of tremella, 5 parts of Aronia melanocarpa fruit powder, 1 part of Guan Shanying pollen and 0.5 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 5min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1), stirring and mixing, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivation, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 1
This comparative example composition differs from example 1 in probiotics. The specific raw materials and the preparation method are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 8 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum LP-115. Among them, lactobacillus plantarum LP-115 was supplied by Danish (China) Inc.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain solution, adding lactobacillus plantarum LP-115 (the mass ratio of lactobacillus plantarum LP-115 to the solution is 0.05:1), and mixing to obtain mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 2
The comparative example composition differs from example 1 in the extraction process, and the specific raw materials and preparation are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 8 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and fructus Rosae Laevigatae powder, adding 1 times of water, adding 1% of mixed enzyme (laccase and 1398 neutral protease with mass ratio of 1:1) by stirring, mixing, performing enzymolysis at 45deg.C and pH7.0 for 2 hr, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution, reflux extracting for 1 hr, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 3
The moisturizing and freckle-removing composition of the comparative example is different from the composition of the example 1 in the extraction process, and the specific raw materials and preparation are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 8 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (1398 neutral protease and soybean peptide extraction enzyme with mass ratio of 1:1), stirring, mixing, performing enzymolysis at 45deg.C and pH7.0 for 2 hr, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1 hr, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 4
The moisturizing and freckle-removing composition of the comparative example is different from the composition of the example 1 in the extraction process, and the specific raw materials and preparation are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 20 parts of tremella, 2 parts of Aronia melanocarpa fruit powder, 8 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivation, filtering to obtain enzymolysis liquid and residue, adding 75% ethanol water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, recovering ethanol from feed liquid, and adding enzymolysis liquid to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 5
The composition of this comparative example differs from that of example 1 in the composition of the raw materials. The specific raw materials and the preparation method are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 30 parts of tremella, and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Comparative example 6
The composition of this comparative example differs from that of example 1 in the composition of the raw materials. The specific raw materials and the preparation method are as follows:
the raw materials are composed of the following components in parts by weight: 30 parts of elastin peptide, 6 parts of Aronia melanocarpa fruit powder, 24 parts of Guan Shanying pollen and 1 part of lactobacillus plantarum S58.
The preparation method comprises the following steps:
(1) Adding 3 times of water into tremella, pulping for 3min to obtain a pulping liquid;
(2) Mixing Aronia melanocarpa fruit powder and sakura powder, adding 1 times of water, adding 1% of mixed enzyme (laccase, 1398 neutral protease and soybean peptide extraction enzyme with the mass ratio of 1:1:1) by stirring, performing enzymolysis at 45deg.C and pH7.0 for 2h, boiling for 2min for inactivating, filtering to obtain enzymolysis solution and residue, adding 3 times of 1% lecithin-containing water solution into residue, reflux extracting for 1h, filtering to obtain feed liquid, and adding enzymolysis solution to obtain extractive solution;
(3) Mixing the extractive solution and the beating solution to obtain a solution, adding lactobacillus plantarum S58 (the mass ratio of lactobacillus plantarum S58 to the solution is 0.05:1), and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution, and homogenizing.
Test I, freckle removing and whitening human body test feeding test
Human body test food test is carried out on the product according to the health food inspection and evaluation criterion
1.1 the subject met the following criteria:
1) Pale brown to dark brown spots on the face, well-defined patches, no inflammatory manifestations;
2) The illness state is seasonal, and the summer is heavy and the winter is light;
3) There is no obvious pigmentation caused by other diseases except endocrine.
1.2 test group: 135 volunteers satisfying the above conditions were aged 32-45 years. When the facial chloasma area, the depth and the like of the facial chloasma are considered as much as possible during grouping, and the outdoor activity conditions, the ages, the sexes, the fertility and the like of each volunteer affecting the results are checked for balance, so that no significant difference (P is more than 0.05) between groups is ensured.
1.3 dose and administration method: each group of volunteers took orally the compositions prepared in examples 1-3 and comparative examples 1-6, respectively, 20mL each time, twice a day, for 2 months in succession, without changing the original eating habits during the trial period.
1.4 evaluation method of effect:
(1) Scale for detecting the area of facial chloasma measures the area (mm) of the whole facial chloasma before and after the test 2 ）；
(2) The detection of the color depth of the facial chloasma is developed according to the design of the geographic research institute of China academy of sciences, and the brown (Y+M+BK, namely yellow+magenta+black color stack) color card in the practical standard color card (first edition) published by the mapping publishing society in 1992 is a judgment standard of the color depth of the chloasma: the degree I (15, 20, 5), the degree II (30, 40, 10), the degree III (40, 60, 15) and the degree I, the degree II and the degree III of the color card are respectively recorded as 1 minute, 2 minutes and 3 minutes, and the color integral and the area change rate of chloasma before and after test feeding are analyzed.
1.5 analysis of results: self-control data were paired t-test.
(1) Safety evaluation: the physiological indexes of the subject are not different from those before trial eating.
(2) Chloasma area reduction condition of volunteers in each experimental group
TABLE 1
(1) Chloasma color change comparison (integral)
TABLE 2
Note that: in contrast to the self-control, * P＜0.01， # P＜0.05。
test two, human body test food skin moisture-preserving elasticity test
110 female volunteers 30-50 years old were randomly selected, and the skin of the volunteers was healthy and free from chronic skin diseases.
Subject exclusion criteria: 1 pregnant or lactating women, allergic constitution and allergy to the health product. 2 are combined with cardiovascular and cerebrovascular diseases, liver diseases, kidney diseases, hematopoietic diseases and mental medical history. 3 taking items related to the tested function for a short period of time affects the judgment of the result. 4. The test sample is not tried according to the experimental requirement, and the efficacy or data inadequacy cannot be judged to affect the efficacy or safety judgment.
Grouping: two control designs, self and inter-group, were used. The skin moisture conditions of the subjects were randomly divided into a group of example 1, a group of example 2, a group of example 3, a group of example 4, a group of example 5, a group of comparative example 1, a group of comparative example 2, a group of comparative example 3, a group of comparative example 4, a group of comparative example 5, a group of comparative example 6, 10 persons each, and examples 1 to 5 were test groups, and the number of subjects was 50. Comparative examples 1-6 were control groups, and the number of subjects in the control groups was 60. As much as possible, the main factors affecting the results, such as age, etc., are considered for the balance check to ensure comparability between groups.
The administration method comprises the following steps: the samples prepared in examples 1-5 and comparative examples 1-6 of the corresponding group were taken once a day, 20mL each time, for 45 days. The subject had to take other items that retain skin moisture during the test period and had no effect on the cosmetic product that affected the outcome determination. The original eating habit is not changed during the test period, and the diet is normal.
Test: the skin between the forehead was tested for moisture. The measurement environment is carried out in a detection room with wide space environment, good ventilation condition, stable temperature, humidity and the like. In a quiet state, the measured part is cleaned by dipping a clean cotton ball in distilled water, the water content is measured 15 minutes after the cleaning, and the measurement work before and after the test is performed by the same instrument and the same person.
Skin moisture and skin elasticity before and after 45 days of test feeding were tested using an MLB-D0028 intelligent skin detector, and then the skin moisture increase% and elasticity increase% were calculated and averaged. As in table 3.
TABLE 3 Table 3
The foregoing detailed description is directed to one of the possible embodiments of the present invention, which is not intended to limit the scope of the invention, but is to be accorded the full scope of all such equivalents and modifications so as not to depart from the scope of the invention.
Claims (10)
1. The composition is characterized by comprising the following raw materials: elastin peptide, tremella, aronia melanocarpa fruit powder, guan Shanying pollen and lactobacillus plantarum S58.
2. The composition according to claim 1, wherein the raw materials of the composition comprise the following components in parts by weight: 20-50 parts of elastin peptide, 1-50 parts of tremella, 0.1-5 parts of Aronia melanocarpa fruit powder, 1-10 parts of Guan Shanying pollen and 0.1-5 parts of lactobacillus plantarum S58.
3. The composition according to claim 2, wherein the raw materials of the composition comprise the following components in parts by weight: 30-40 parts of elastin peptide, 10-20 parts of tremella, 0.5-2 parts of Aronia melanocarpa fruit powder, 5-10 parts of Guan Shanying pollen and 0.5-2 parts of lactobacillus plantarum S58.
4. The composition of claim 1, wherein lactobacillus plantarum S58 is an inactivated lactobacillus with a strain concentration of 4000 hundred million CFU/g before inactivation.
5. The composition of claim 1, wherein the composition further comprises a flavoring agent; the correctant is one or two selected from citric acid, sucralose and mogroside.
6. A process for the preparation of a composition as claimed in any one of claims 1 to 5, comprising the steps of:
(1) Pulping tremella to obtain pulp;
(2) Mixing and extracting Aronia melanocarpa fruit powder and flos Rosae Laevigatae powder to obtain extractive solution;
(3) Mixing the extract with the beating solution to obtain a solution, then adding lactobacillus plantarum S58, and mixing to obtain a mixed solution;
(4) Mixing elastin peptide and the mixed solution or adding correctant and mixing.
7. The method according to claim 6, wherein in the step (1), the beating is carried out by adding 3-5 times of water and beating for 3-5min; in the step (2), the extraction is heating reflux extraction, and the extraction time is 1-2h.
8. The method according to claim 6, wherein in the step (2), the step of extracting is carried out by performing enzymolysis, filtering to obtain an enzymolysis liquid and a residue, adding an aqueous solution containing 0.5-1% lecithin into the residue, extracting to obtain a feed liquid, and adding the enzymolysis liquid to obtain an extract.
9. The method according to claim 8, wherein the enzyme used for the enzymolysis comprises laccase, 1398 neutral protease and soybean peptide extracting enzyme in a mass ratio of 1:0.5-1:0.5-1; the enzymolysis pH is 5-7, the enzymolysis temperature is 30-50 ℃, and the enzymolysis time is 1-3h.
10. Use of a composition according to any one of claims 1 to 5 or a composition according to any one of claims 6 to 9 in the preparation of a product for promoting collagen regeneration, moisturizing, improving skin elasticity and/or improving skin chloasma.
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