KR20080049176A - Food for beauty to improve moisture of skin - Google Patents

Abstract

A cosmetic food composition with skin moisturization effect containing elastin as an active ingredient is provided to be applied to a health food for safe skin cosmetic purpose without worry of adverse reactions or poisoning while preventing skin damage by ultraviolet rays and providing moisturization effect. A cosmetic food composition with skin moisturization effect contains 10 to 100% by weight of elastin comprising peptide with a molecular weight of less than 5,000 Daltons. The cosmetic food composition is formulated into a pill, tablet, soft gelatin capsule, granule or drink. For an example, 30% by weight of elastin is mixed with 30% by weight of corn starch, 20% by weight of glycerin and 20% by weight of sorbitol powder and forming into a pill.

Description

Beauty food composition with skin moisturizing effect {Food for beauty to improve moisture of skin}

1 is a graph showing the molecular weight distribution of elastin according to the present invention.

Figure 2 is a graph showing the results of measuring the transdermal water loss (Trans-Epidermal Water Loss, TEWL) after ingesting the elastin according to the present invention in a hairless mouse (Hairless mouse) that damaged the skin by irradiation with ultraviolet rays .

Figure 3 is the result of analyzing the hydroxyproline (hydroxyproline) content present in the skin tissue of the hairless mice ingested elastin according to the present invention (% by weight).

Figure 4 shows the results of H & E staining and Masson-Trichrome staining of the skin tissue of the elastin-ingested hairless mice according to the present invention as an enlarged photograph through an optical microscope.

The present invention relates to a cosmetic food composition for improving skin moisturizing containing elastin as an active ingredient, and more particularly, contains elastin and helps in the production of skin constituents by ingesting it, and the skin barrier caused by ultraviolet rays. It relates to a cosmetic food composition having a moisturizing effect to prevent damage to maintain the moisture of the skin epidermis.

For the beauty of the skin, there are two options, external and oral, as a means of supplying the active ingredients necessary for the skin. Since skin is an organ that is in contact with the outside world, most of the external methods of applying cosmetics or medicines to the surface of the skin to transfer useful ingredients through the surface are taken up. However, the skin is also less than expected to be absorbed through such barriers, even though it is a beneficial component because the skin surface also acts as a kind of barrier against invasion of foreign substances. Therefore, it is effective not only to reach the skin through the digestive tract through the digestive tract through the oral intake of the skin but also to apply the external and oral intake together to solve the underlying problem of the skin. It is considered reasonable. Therefore, as the importance of skin beauty by oral ingestion is recently recognized, both demand and supply of skin beauty foods are increasing, and skin beauty products using both cosmetic use and oral intake of food are being released. It is called a two-way product.

Human skin is degraded by external factors such as ultraviolet rays, environmental pollution, and internal factors such as age increase and mental stress, and the normal function of the skin is degraded. Especially, ultraviolet rays are the biggest cause of skin aging and damage. It is known as a factor. Skin exposed to UV rays may cause aging such as wrinkle formation, loss of elasticity and pigmentation, as well as a decrease in skin moisture due to damage to the skin barrier. One of the areas of greatest interest in skin aging and cosmetic research is to prevent such skin damage by ultraviolet light. When the skin surface moisture content decreases due to the skin damage caused by ultraviolet rays, as a result of this, the flexibility of the stratum corneum of the skin surface is lost, resulting in dry or rough skin. Therefore, in order to maintain healthy skin, it is very important to maintain the moisture of the skin, that is, the skin moisturizing power.

It is a natural moisturizing factor (NMF) that exists in the stratum corneum of the skin surface to play an important role in maintaining the skin moisturizing power. The main component of NMF is free amino acid, which accounts for 40% of NMF, and contains many amino acids such as serine, glycine, alanine, and citrulline. The amount of free amino acids in the stratum corneum correlates with the moisture content of the epidermis, and studies have shown that the amino acid composition in the skin has changed in skin with reduced moisture. Therefore, it is important to maintain the amount of amino acids in the NMF in order to increase the moisture retention of the skin surface.

Amino acids are not only constituents of NMF, but also most of the components except moisture in the whole skin tissue. In order to maintain the skin's moisturizing power and to maintain the health of the skin, it is possible to think of a method of ingesting a direct intake of amino acids, ingesting a component rich in amino acids, or ingesting a component that can help synthesize these amino acids in vivo.

Considering the importance of amino acids in skin care, a method of externally adding amino acid to cosmetics has been used in the past. However, these products have the disadvantage of acting only on the application site, and to compensate for this disadvantage, a systemic supply of amino acids through food is required. The supply may be through amino acid supplements, but in the case of beauty foods for skin health, more attention is focused on peptides that combine various amino acids and protein components that combine these peptides. The reason is that peptides and protein components sometimes show better efficacy than consuming free amino acids. One of the most representative examples is collagen peptide. It is known that ingesting collagen peptides is more useful to the human body than eating a mixture of amino acids in the same free form as the amino acid composition of collagen peptides. Like collagen peptides, peptide-type components that hydrolyze proteins to facilitate digestion and absorption It is attracting attention as a skin beauty food material.

Therefore, the present inventors introduced elastin (or elastin peptide) having a molecular weight lower than 5000 Daltons or less into food to facilitate digestion and absorption by allowing the polymer protein to exist in the form of peptide through hydrolysis. The present invention finds that it is possible to prepare a cosmetic food composition having a moisturizing effect of supplying raw materials of protein and protein, helping their production in the body, preventing skin damage caused by ultraviolet rays and ultimately maintaining moisture of the skin. Completed.

Accordingly, it is an object of the present invention to provide a cosmetic food composition containing elastin to help the production of skin constituents, and to provide a moisturizing effect so that the skin can retain moisture by preventing skin damage caused by ultraviolet rays.

In order to achieve the above object, the present invention provides a cosmetic food composition having a skin moisturizing effect, comprising elastin as an active ingredient.

In the present invention, the elastin has a molecular weight of 5000 Daltons or less.

In addition, the elastin in the present invention is characterized in that it contains 10 to 100% by weight.

The cosmetic food composition according to the present invention is applicable to the food of various formulations, such as tablets, capsules, pills, granules, drinks.

Hereinafter, the present invention will be described in more detail.

In the present invention, the active ingredient used to provide a skin moisturizing effect is elastin. Elastin used in the present invention is known as a component of the constituent proteins in the body, which together with collagen make up the intercellular matrix of animal connective tissue. Elastin is widely present in elastic tissues such as blood vessel walls, lungs, and skin. The elastin used in the present invention is made of prolastin (Marine Elastin Powder) manufactured by CTPP (France). Prolastin® is a raw material prepared by extracting elastin from fish skin through enzymatic hydrolysis and filtering, deodorizing and powdering process. As can be seen in FIG. It does not exceed Daltons, and consists of peptide components with an average molecular weight of about 1400 Daltons. Therefore, elastin used in the present invention can also be referred to as elastin peptide.

The amino acid composition of elastin is 21.8% glycine, 10.7% glutamic acid, 10.2% proline, 7.9% alanine, 7.8% arginine, 6.7% serine, 6.6% aspartic acid, 5.3% hydroxyproline and the like. This raw material has a moisture content of less than 8% and a total nitrogen content of 12% or more.

The composition of the present invention contains 10 to 100% by weight of such elastin. When the elastin content is less than 10% by weight it is low to show the efficacy to include more than 10% by weight, the content of elastin 100% by weight of the formulation is prepared in the form of powder or granules and contains other auxiliary ingredients If not, it means.

The composition of the present invention can be used as a health food by properly formulating auxiliary ingredients generally used in the art, in addition to the above-mentioned ingredients, in the form of tablets, hard capsules, soft capsules, pills, granules, drinks, and the like. have.

The present invention will be described in more detail with reference to the following Examples. However, these examples are intended to illustrate the present invention, it is apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

[Test Example 1]

In order to evaluate the effect of the composition of the present invention on the skin damage and skin moisturizing caused by UV light, 14 weeks of testing was conducted on hairless mice. Seven female 8-week-old female hairless mice (SKh: HR-1) per group, control group (untreated group), UV / control group (UV control group), collagen peptide group (positive control group), elastin (Elastin) The test was performed by dividing the group into groups.

As a positive control, collagen peptides, which are well known as skin care materials, were taken and the test results were compared with the elastin intake groups.

During the first 7 weeks after the start of the test, the sample was not administered, and only ultraviolet rays were irradiated three times a week through an ultraviolet lamp (Waldmann UV800, Germany). Through this, after causing skin damage, the samples were orally administered with UV irradiation for the remaining seven weeks, and the dosage of each sample is shown in Table 1. The dose of this test was determined to be the optimal dose after deriving a dosage range that showed useful effects through previous experiments. During the test period, the hairless mice were given free food and water.

Test Group Composition and Sample Dosage Test group name UV irradiation Dosage The number of animals Control group X Saline 10 ml / kg B.W. 7 UV / Control group O Saline 10 ml / kg B.W. 7 Collagen Peptides O 1000 mg / kg B.W. 7 Elastin O 1000 mg / kg B.W. 7

(B.W. = Body weight)

The control group and the UV / Control group were orally administered saline 5 times a week for 7 weeks using a mouse antagonist at a dose of 10 ml per unit weight (kg). The collagen peptide group and the elastin group were dissolved orally in saline (10 ml per unit weight (kg)) of the doses listed in Table 1 and administered orally five times a week in the same manner as the control group. The total UV dose during the test period was irradiated to 7095 mJ / cm ² .

14 weeks after the start of the test, trans-Epidermal Water Loss (TEWL) was measured using VapoMeter® on the back skin of hairless mice of each test group, and the results are shown in FIG. 2. The environmental conditions at the time of measurement were 60 ± 5% relative humidity and 25.5 ± 0.5 ° C.

Transdermal moisture loss is an indicator used to determine skin barrier function, recovery after skin barrier damage, and skin protection against stimuli such as ultraviolet light. The epidermal moisture loss, or TEWL value, is the amount of water emanating from the skin, and the higher this value, the less moisturizing the skin has, and at the same time, the intrinsic barrier function of the skin is impaired.

From the graph of Figure 2 it was confirmed that the skin moisturizing effect to prevent skin moisture loss caused by ultraviolet rays. It can be seen that the TEWL value is the highest in the UV / Control group, indicating that the UV barrier damages the skin barrier and impairs skin moisturizing function. In contrast, the control group with normal skin without UV irradiation had the lowest TEWL, indicating that skin moisture retention is higher than that of the other groups. The * notation on the graph indicates that the TEWL of the elastin group was measured statistically significantly at the p <0.05 level compared to UV / Control, indicating that the intake of elastin is effective in maintaining skin moisture. TEWL of the collagen peptide group was also lower than that of UV / Control, but there was no significant difference.

[Test Example 2]

To determine the effect of ingestion of elastin on the skin constituents, skin tissue of the back region of hairless mice belonging to each test group obtained in Test Example 1 was collected, and then the amount of hydroxyproline present in the skin was measured. It was. Hydroxyproline is a type of amino acid that is a constituent of skin proteins. In the body, hydroxylase synthesizes this amino acid by attaching -OH group to proline. In the test method of Test Example 2, the skin tissue was hydrolyzed with 6N hydrochloric acid solution, filtered, diluted and derivatized, and then hydroxyproline was quantified using HPLC.

The results are shown in FIG. 3, where the hydroxyproline content is expressed as% of skin tissue weight. The * notation on the graph means that there is a statistically significant difference at p <0.05 compared to the UV / Control group. As can be seen in Figure 3 compared to the other test group in the elastin (Elastin) group the content of hydroxyproline in the skin tissue was significantly increased. From the test results, two possible mechanisms can be expected for the increase in hydroxyproline content by ingesting elastin. The first is to feed elastin composed of various amino acids into the body, and the second is to lower the molecular weight of peptide breakdown products produced by digestion after ingesting elastin. It is a possibility that it can act as a factor to promote the production of skin constituents such as oxyproline. Either way, ingestion of elastin helped the production of constituent amino acids and prevented the loss of moisture caused by skin damage caused by UV rays, which helped maintain normal skin condition.

In the group ingested collagen peptide, the hydroxyproline content was significantly increased compared to the UV / Control group, but the effect was lower than that of elastin.

[Test Example 3]

In order to evaluate the effect of elastin ingestion on skin damage by ultraviolet rays in more detail through tissue staining, staining was performed on the back skin tissue of the hairless mice obtained in Test Example 1 above. The animal was sacrificed to prepare a tissue sample, and then the back skin was harvested to obtain 2 X 2 cm ² Attached to a sized filter paper (fixed paper), fixed in 10% neutral formalin and subjected to a conventional tissue treatment, paraffin embedded, cut into 4 ㎛ sections and subjected to H & E (Hematoxylin & Eosin) staining and Masson-Trichrome staining.

The staining results are shown in the optical microscope magnification of FIG. 4. Compared to the normal skin condition of the control group, the UV / Control group showed abnormally increased epidermal thickness and clear staining of the upper dermal layer. In contrast, the elastin group showed little damage to the dermal layer and had a similar cross-section to the control group. The test also shows that elastin intake is useful to prevent damage to the skin barrier caused by UV light.

Next, the food composition prepared by containing the elastin according to the present invention will be described in more detail by way of example. However, the present invention is not limited only to these formulation examples.

Formulation Example 1 Soft Capsule

Elastin 30% by weight, soybean oil 32.8% by weight, 2.2% by weight of sulfur, 2% by weight of lecithin, 1% by weight of vitamin C was mixed, and the contents were filled in 400mg of 32% by weight of gelatin capsules to prepare a soft capsule.

Formulation Example 2 Preparation of Pill

Elastin 30% by weight, corn starch 30% by weight, glycerin 20% by weight, sorbitol powder 20% by weight were mixed, and the ring was prepared using a stirrer. The final weight of the contents was 3.5 g.

Formulation Example 3 Tablet

30% by weight elastin, 20.5% by weight lactose, 20% by weight dextrin, 20% by weight maltitol powder, 7% by weight xylitol powder was granulated using a fluidized bed dryer and then 2.5% by weight sugar ester It was added and tableted with a tableting machine. The final weight of the content was 2 g.

Formulation Example 4 Granules

30% by weight of elastin, 5% by weight of xylitol and 65% by weight of isomalt were mixed and molded into granules using a fluid bed granulator before filling into the fabric. The final weight of the content was 2 g.

Formulation Example 5 Drinking Agent

5% by weight of elastin, 5% by weight of maltitol syrup, and 0.1% by weight of citric acid were mixed, and then 1200ml of purified water was added thereto so as to fill each bottle with 120ml. After filling the bottle was sterilized for 2-3 hours at 90 ℃ to prepare a beverage.

As described above, the cosmetic food composition according to the present invention assists in the production of skin constituents, prevents skin damage caused by ultraviolet rays, and provides a moisturizing effect to keep the skin hydrated.These pills, capsules, tablets, Formulated with granules, drinks, etc., it can be applied as a health food for safe skin beauty without side effects or poisoning.

Claims (6)

A cosmetic food composition comprising elastin as an active ingredient. The cosmetic food composition of claim 1, wherein the elastin is composed of peptides having a molecular weight of 5000 Daltons or less. The cosmetic food composition of claim 1, wherein the elastin is contained in an amount of 10 to 100% by weight. According to claim 1, wherein the composition is a cosmetic food composition, characterized in that to help the production of skin constituent amino acids and prevent skin damage by ultraviolet light. The cosmetic food composition of claim 1, wherein the composition has a function of improving skin moisturizing ability. The cosmetic food composition of claim 1 wherein the composition is formulated in a formulation of pills, tablets, hard capsules, soft capsules, granules or drinks.

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