CN117598301A - Preparation for influencing leafhopper trend behavior and application of preparation in leafhopper attraction or avoidance - Google Patents
Preparation for influencing leafhopper trend behavior and application of preparation in leafhopper attraction or avoidance Download PDFInfo
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- CN117598301A CN117598301A CN202311611756.5A CN202311611756A CN117598301A CN 117598301 A CN117598301 A CN 117598301A CN 202311611756 A CN202311611756 A CN 202311611756A CN 117598301 A CN117598301 A CN 117598301A
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- Prior art keywords
- leafhoppers
- sabinene
- acetophenone
- eucalyptol
- leafhopper
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- 241001414720 Cicadellidae Species 0.000 title claims abstract description 135
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- NDVASEGYNIMXJL-UHFFFAOYSA-N sabinene Chemical compound C=C1CCC2(C(C)C)C1C2 NDVASEGYNIMXJL-UHFFFAOYSA-N 0.000 claims abstract description 128
- KWOLFJPFCHCOCG-UHFFFAOYSA-N Acetophenone Chemical compound CC(=O)C1=CC=CC=C1 KWOLFJPFCHCOCG-UHFFFAOYSA-N 0.000 claims abstract description 124
- NDVASEGYNIMXJL-NXEZZACHSA-N (+)-sabinene Natural products C=C1CC[C@@]2(C(C)C)[C@@H]1C2 NDVASEGYNIMXJL-NXEZZACHSA-N 0.000 claims abstract description 64
- KQAZVFVOEIRWHN-UHFFFAOYSA-N alpha-thujene Natural products CC1=CCC2(C(C)C)C1C2 KQAZVFVOEIRWHN-UHFFFAOYSA-N 0.000 claims abstract description 64
- 229930006696 sabinene Natural products 0.000 claims abstract description 64
- WEEGYLXZBRQIMU-UHFFFAOYSA-N 1,8-cineole Natural products C1CC2CCC1(C)OC2(C)C WEEGYLXZBRQIMU-UHFFFAOYSA-N 0.000 claims abstract description 50
- WEEGYLXZBRQIMU-WAAGHKOSSA-N Eucalyptol Chemical compound C1C[C@H]2CC[C@]1(C)OC2(C)C WEEGYLXZBRQIMU-WAAGHKOSSA-N 0.000 claims abstract description 50
- 229960005233 cineole Drugs 0.000 claims abstract description 50
- 239000005667 attractant Substances 0.000 claims abstract description 35
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 claims abstract description 34
- NDJKXXJCMXVBJW-UHFFFAOYSA-N heptadecane Chemical compound CCCCCCCCCCCCCCCCC NDJKXXJCMXVBJW-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000031902 chemoattractant activity Effects 0.000 claims abstract description 26
- HFPZCAJZSCWRBC-UHFFFAOYSA-N p-cymene Chemical compound CC(C)C1=CC=C(C)C=C1 HFPZCAJZSCWRBC-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 17
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 17
- 229930007744 linalool Natural products 0.000 claims abstract description 17
- GRWFGVWFFZKLTI-UHFFFAOYSA-N α-pinene Chemical compound CC1=CCC2C(C)(C)C1C2 GRWFGVWFFZKLTI-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000004480 active ingredient Substances 0.000 claims abstract description 15
- 229930006978 terpinene Natural products 0.000 claims abstract description 15
- 150000003507 terpinene derivatives Chemical class 0.000 claims abstract description 15
- 239000001278 2-(5-ethenyl-5-methyloxolan-2-yl)propan-2-ol Substances 0.000 claims abstract description 13
- BRHDDEIRQPDPMG-UHFFFAOYSA-N Linalyl oxide Chemical compound CC(C)(O)C1CCC(C)(C=C)O1 BRHDDEIRQPDPMG-UHFFFAOYSA-N 0.000 claims abstract description 13
- GRWFGVWFFZKLTI-IUCAKERBSA-N 1S,5S-(-)-alpha-Pinene Natural products CC1=CC[C@@H]2C(C)(C)[C@H]1C2 GRWFGVWFFZKLTI-IUCAKERBSA-N 0.000 claims abstract description 11
- MVNCAPSFBDBCGF-UHFFFAOYSA-N alpha-pinene Natural products CC1=CCC23C1CC2C3(C)C MVNCAPSFBDBCGF-UHFFFAOYSA-N 0.000 claims abstract description 9
- 150000007823 ocimene derivatives Chemical class 0.000 claims abstract description 8
- XJPBRODHZKDRCB-UHFFFAOYSA-N trans-alpha-ocimene Natural products CC(=C)CCC=C(C)C=C XJPBRODHZKDRCB-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims description 10
- 229940044949 eucalyptus oil Drugs 0.000 claims description 8
- 239000010642 eucalyptus oil Substances 0.000 claims description 8
- 239000005871 repellent Substances 0.000 claims description 6
- 230000002940 repellent Effects 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 2
- 241000086608 Empoasca vitis Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 241000607479 Yersinia pestis Species 0.000 abstract description 5
- 230000006399 behavior Effects 0.000 description 22
- 238000012360 testing method Methods 0.000 description 21
- 238000011282 treatment Methods 0.000 description 21
- 150000001875 compounds Chemical class 0.000 description 19
- 241001122767 Theaceae Species 0.000 description 17
- 235000013616 tea Nutrition 0.000 description 17
- 241000238631 Hexapoda Species 0.000 description 10
- 229940057995 liquid paraffin Drugs 0.000 description 10
- 239000011550 stock solution Substances 0.000 description 8
- 240000001548 Camellia japonica Species 0.000 description 7
- 235000018597 common camellia Nutrition 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 244000001582 Canarium odontophyllum Species 0.000 description 6
- 235000016299 Canarium odontophyllum Nutrition 0.000 description 6
- UAHWPYUMFXYFJY-UHFFFAOYSA-N beta-myrcene Chemical compound CC(C)=CCCC(=C)C=C UAHWPYUMFXYFJY-UHFFFAOYSA-N 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000000546 chi-square test Methods 0.000 description 6
- IIYFAKIEWZDVMP-UHFFFAOYSA-N tridecane Chemical compound CCCCCCCCCCCCC IIYFAKIEWZDVMP-UHFFFAOYSA-N 0.000 description 6
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 5
- 240000003553 Leptospermum scoparium Species 0.000 description 5
- 235000015459 Lycium barbarum Nutrition 0.000 description 5
- 241001477931 Mythimna unipuncta Species 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 239000003039 volatile agent Substances 0.000 description 4
- VYBREYKSZAROCT-UHFFFAOYSA-N alpha-myrcene Natural products CC(=C)CCCC(=C)C=C VYBREYKSZAROCT-UHFFFAOYSA-N 0.000 description 3
- 229930007927 cymene Natural products 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 230000030786 positive chemotaxis Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 240000001238 Gaultheria procumbens Species 0.000 description 2
- 235000007297 Gaultheria procumbens Nutrition 0.000 description 2
- 244000082204 Phyllostachys viridis Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 241000255969 Pieris brassicae Species 0.000 description 2
- 235000010394 Solidago odora Nutrition 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 235000020334 white tea Nutrition 0.000 description 2
- WAPNOHKVXSQRPX-UHFFFAOYSA-N 1-phenylethanol Chemical compound CC(O)C1=CC=CC=C1 WAPNOHKVXSQRPX-UHFFFAOYSA-N 0.000 description 1
- 101100394003 Butyrivibrio fibrisolvens end1 gene Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000995023 Empoasca Species 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 231100000703 Maximum Residue Limit Toxicity 0.000 description 1
- 241000192701 Microcystis Species 0.000 description 1
- 241000409991 Mythimna separata Species 0.000 description 1
- 244000155788 Strobilanthes moschifera Species 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 208000009091 myxoma Diseases 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P17/00—Pest repellants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparation for influencing the trending behavior of leafhoppers and application thereof in the attraction or avoidance of leafhoppers, and belongs to the technical field of pest control. The invention relates to a preparation for influencing the trending behavior of leafhoppers, and the active ingredients comprise at least one of acetophenone, terpinene, linalool, ocimene, linalool oxide, alpha-pinene, sabinene, eucalyptol, heptadecane and p-cymene. The combined and screened attractant has obvious attracting effect on leafhoppers, and can be applied to leafhopper attracting in a field after being prepared into a preparation, so that the effect is obvious.
Description
Technical Field
The invention belongs to the technical field of pest control, and particularly relates to a preparation for influencing the trending behavior of leafhoppers and application of the preparation in the attraction or the avoidance of the leafhoppers.
Background
The sucking mouthparts pests such as leafhoppers are dominant species of tea garden pests, and in recent years, the outbreak frequency of the sucking mouthparts pests in tea gardens is increased, so that the development of tea industry is seriously affected. The current control measures are mainly chemical control, however, the use of excessive pesticides not only leads to high levels of insect resistance to various pesticides, but also causes serious environmental pollution, so that the pesticide residue level of tea leaves is extremely easy to exceed the maximum residue limit of tea products. Therefore, a new way for preventing and treating leafhoppers with high efficiency, safety and harmlessness is urgently needed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a preparation for influencing the trending behavior of leafhoppers and application thereof in the attraction or avoidance of leafhoppers.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a preparation for influencing leafhopper trend comprises at least one of acetophenone, terpinene, linalool, ocimene, linalool oxide, alpha-pinene, sabinene, eucalyptol, heptadecane and p-cymene as effective components; preferably, the active ingredient comprises at least one of acetophenone, terpinene, linalool, sabinene, eucalyptol, heptadecane and p-cymene; still more preferably, the active ingredient is at least two of acetophenone, sabinene and eucalyptol; the effect on the leafhopper trend behavior is that the leafhopper is subjected to trapping or evasion.
Based on the scheme, the concentration of the active ingredient in the preparation for influencing the leafhopper trend behavior is 0.001
μL/mL～1000μL/mL。
The application of the preparation affecting the leafhopper trend behavior in preparing leafhopper attractants or leafhopper repellents.
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is as follows: 2:1:1, 10:1:1 or 60:1:1, the working concentration is 0.1 mu L/mL when the ratio is 2:1:1, the working concentration is 0.1 mu L/mL to 1000 mu L/mL when the ratio is 10:1:1, and the working concentration is 1000 mu L/mL when the ratio is 60:1:1;
an leafhopper attractant contains acetophenone and sabinene as effective components; wherein, the volume ratio of the acetophenone sabinene is as follows: 1:1, working concentration was 1000. Mu.L/mL.
An leafhopper attractant contains eucalyptol and sabinene as effective components; wherein, the volume ratio of the eucalyptol to the sabinene is as follows: 1:1, the working concentration is 0.001 mu L/mL-1000 mu L/mL.
The leafhopper repellent comprises the following active ingredients of acetophenone, eucalyptol and sabinene in a volume ratio: 2:1:1 or 60:1:1, when the volume ratio of acetophenone, eucalyptol and sabinene is: at a ratio of 2:1:1, the working concentration is 1000. Mu.L/mL, and when the volume ratio of acetophenone, eucalyptol and sabinene is: at 60:1:1, the working concentration was 0.001. Mu.L/mL.
The application of the attractant or the repellent in the control of leafhoppers, wherein the leafhoppers are small-tubular leafhoppers.
The beneficial effects of the invention are as follows:
the invention provides a preparation for influencing the trending behavior of leafhoppers, which comprises at least two of acetophenone, eucalyptol and sabinene as active ingredients, has obvious attraction or avoidance effect on the leafhoppers, and can be applied to leafhopper attraction or avoidance in fields after being prepared into the preparation, and has obvious effect. The active ingredients of the preparation are derived from plants, so that the use of pesticides can be reduced, and the preparation has better ecological benefit.
Drawings
FIG. 1 is a total ion flow chromatogram of the volatile components of 'Fuding Dabai' camellia;
FIG. 2 is a behavioral response of adult small leafhoppers to single component volatile diluted solutions (x represents a significant difference in the number of insects tending to the treatment and control (P <0.05; χ2-test); x represents a significant difference in the number of insects tending to the treatment and control (P <0.01; χ2-test));
FIG. 3 shows the field control effect of 13 components in the tea volatiles on tea garden microcystis minor leafhoppers (wherein, the abscissas 1-14 represent acetophenone, terpinene, phenethyl alcohol, linalool, ocimene, linalool oxide, myrcene, alpha-pinene, sabinene, eucalyptol, tridecane, heptadecane, P-cymene, liquid paraffin respectively; error bars represent standard errors between replicates, the same letters represent no significant difference between the means (P > 0.05), and the different letters represent significant differences between the means (P <0.05, one-way ANOVA));
FIG. 4 is a plot of the behavioral response of the leafhoppers to the volatile stock of the 10 mixture components (x represents a significant difference in the number of insects tending to the treatment and control (P <0.05; χ2-test); x represents a significant difference in the number of insects tending to the treatment and control (P <0.01; χ2-test));
FIG. 5 is a plot of the behavioral response of the small leafhoppers to the diluted solution of the 4 mixed component volatiles (x represents a significant difference in the number of insects tending to the treatment and control (P <0.05; χ2-test); x represents a significant difference in the number of insects tending to the treatment and control (P <0.01; χ2-test));
fig. 6 shows the field control effect of 10 mixed formulations on tea garden leafhoppers (error bars indicate standard errors between replicates, identical letters indicate no significant differences between means (P > 0.05), different letters indicate significant differences between means (P <0.05, one-way ANOVA)).
Detailed Description
Other terms used in the present invention, unless otherwise indicated, generally have meanings commonly understood by those of ordinary skill in the art. The invention will be described in further detail below in connection with specific embodiments and with reference to the data. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
1. Determination of composition and release amount of 'Fuding Dabai' camellia volatile matter
The volatile matter of the large white tea flower of the Fuding is collected by adopting a dynamic headspace absorption method, and the collected volatile matter of the large white tea flower of the Fuding is analyzed by GC-MS (figure 1), and finally 13 main volatile compound components (alpha-pinene, sabinene, myrcene, p-cymene, eucalyptol, ocimene, terpinene, alpha-phenethyl alcohol, linalool, acetophenone, linalool oxide, tridecane, heptadecane and the like) are identified, wherein the types of the compounds are alcohol, alkane, ketone and terpene. Wherein acetophenone is highest in tea flower volatile, and the release amount is 2437.86 + -320.24 ng/hr (Table 1).
TABLE 1 main ingredients and relative content of Fuding Dabai tea flower volatile
2. Different camellia volatile matters have attractive effect on leafhoppers (indoor behavior test)
Indoor behavioral trephine assay to determine behavioral response of Empoasca parviflora to 3 concentration levels of 13 camellia volatile components
An "Y" olfactometer was used to determine the trendy of 3 concentrations of adult leafhoppers to 13 compounds. Each compound was diluted at 10, 0.1 and 0.001 μl/mL in liquid paraffin, respectively. In each test, 100. Mu.L of the test compound was dropped onto filter paper as the source of the treatment odor, and 100. Mu.L of liquid paraffin was used as a control.
The test was carried out in a dark laboratory at 25.+ -. 2 ℃ and humidity 70.+ -. 5%. A glass "Y" type tube olfactometer (inner diameter 3cm, diameter length 14cm, arm length 10cm, inner angle 120 ℃) was placed in a dark observation chamber (80X 50X 60 cm) 3 ) Is a kind of medium. An 18W cold white fluorescent tube was positioned above to provide uniform illumination. One side arm was connected to a glass flavor source bottle containing the test compound and the other side arm was connected to a control flavor source bottle containing liquid paraffin. An electric vacuum pump is connected to two side arms of the Y-shaped olfactometerAnd (3) upper part. The inlet air is filtered by activated carbon, the flow meter controls the flow and humidified with a water-absorbing cotton ball. All parts are connected by teflon tubing. The air flow through each arm was maintained at 300mL/min. Before each test, a photometer (TES
1332a, tes electronics, taiwan) measures the light intensity on each arm to be consistent (about 100 lux) and adjusts the "Y" tube until the intensity of both arms and inlet are similar. 1/4 (4.5X4.5) pi cm before introducing leafhoppers 2 100. Mu.L of test compound or control solution (liquid paraffin) was placed in a glass flavor source bottle connecting the arms. Leafhoppers were starved for 2h prior to olfactory testing. Adult leafhoppers per head were used only once in one trial. The behaviour of the leafhoppers was then observed and the choice of smell of the arm of the "Y" type olfactometer was recorded. When the leafhoppers did not reach the mark point within 5min, it was recorded as "no reaction". The leafhoppers were tested, and the number of leafhoppers tested per compound was 30. The odor source was swapped after testing 5 leafhoppers to eliminate the effects of unpredictable asymmetry in the "Y" tube setup. Each compound odor required 30 leafhoppers to successfully make the selection. After one compound was tested, the "Y" olfactometer and glass container were rinsed with 98% acetone and then dried in a 100℃ oven for 2 hours. Activated carbon is re-activated for 4 hours at 100 ℃ and then is reused.
The results are shown in figure 2, with the results that the small leafhoppers had positive chemotaxis to sabinene, heptadecane, linalool, eucalyptol, terpinene, acetophenone and cymene, while there was no apparent trend reaction to alpha-pinene, linalool oxide, myrcene, phenethyl alcohol, ocimene and tridecane. Among the 7 tea volatile components with positive chemotaxis of the small leafhoppers, 3 compounds of sabinene, heptadecane and linalool are the most attractive. Among the 3 concentration levels determined, leafhoppers showed a significant or very significant positive chemotaxis for 2 concentration levels of sabinene, heptadecane and linalool, respectively, i.e., sabinene was selected by the leafhoppers of the small tubular (0.1. Mu.L/mL: χ) 2 ＝10.221,df＝1,P<0.01；10μL/mL：χ 2 ＝18.484,df＝1,P<0.01 Heptadecane (0.001 μl/mL): x-shaped articles 2 ＝10.221,df＝1,P<0.01；10μL/mL：χ 2 ＝10.221,df＝1,P<0.01 Linalool (0.1 μl/mL): x-shaped articles 2 ＝7.521,df＝1,P<0.01 Very significantly higher than the control); the leafhoppers selected 0.001. Mu.L/mL linalool amounts significantly higher than the control (χ 2 =5.272, df=1, p=0.022). Wherein, the highest reaction rate of the leafhoppers to 10 mu L/mL sabinene can reach 80 percent. In addition, ealia minor A.parvifolia was treated with eucalyptol (0.001. Mu.L/mL: χ 2 =5.272, df=1, p=0.022), terpinene (0.1 μl/mL: x-shaped articles 2 =5.272, df=1, p=0.022), acetophenone (0.1 μl/mL: x-shaped articles 2 =5.272, df=1, p=0.022) and p-cymene (0.1 μl/mL: x-shaped articles 2 =5.272, df=1, p=0.022) significantly tended to test compound compared to control. The regulating activity concentration of different volatile compounds on the small leafhoppers is different, the behavior selection concentration of the small leafhoppers on terpinene, acetophenone and cymene is 0.1 mu L/mL, and the eucalyptol with the lowest concentration (0.001 mu L/mL) shows remarkable behavior regulating activity on the small leafhoppers. The small-tubular leafhoppers show a certain negative trend towards 10 mu L/mL alpha-pinene and 0.001 mu L/mL linalool oxide, the reaction rate is 63%, but the difference is not obvious compared with the control (χ 2 ＝2.93,df＝1,P>0.05)。
As can be seen from the above test of the trend behavior of different compounds against leafhoppers, the leafhoppers tend to have a certain concentration threshold for tea volatiles with regulatory action against leafhoppers than the control, such as sabinene (10, 0.1. Mu.L/mL), heptadecane (10, 0.001. Mu.L/mL), linalool (0.1, 0.001. Mu.L/mL), eucalyptol (0.001. Mu.L/mL), terpinene (0.1. Mu.L/mL), acetophenone (0.1. Mu.L/mL) and cymene (0.1. Mu.L/mL).
3. Regulating and controlling effect of different tea volatile matters on tea leafhoppers in tea garden (field trapping test)
The 13 compounds were diluted with liquid paraffin and then formulated into four concentrations of 1000, 10, 0.1, 0.001. Mu.L/mL for testing. At each test, 750 μl of each solution was pipetted into a 2mL capacity slow release bottle as a treatment odor source and 750 μl liquid paraffin as a control. The experiment is carried out in Shandong Taishan mountain tea valley agriculture development limited company, and the tea tree variety is Fuding Dabai'. Yellow adhesive plates (25 cm. Times.20 cm) for field trap experiments were purchased from Engell agriculture technologies Inc. of Zhangzhou. In the field test, the slow release bottle was attached to the upper edge center of a double-sided yellow adhesive plate by a filament, and each adhesive plate was repeated 3 times, using a completely random design. These yellow plates were hung on bamboo poles with the bottom just above the tea tree crown, with a 3m interval between treatments, and a blank control was set. The number of small through leafhoppers on yellow plates was counted after 3d and 7 d.
As shown in fig. 3, the investigation result of 3d after the treatment shows that the number of small through leafhoppers on the sticky plates of 1000 μl/mL ocimene, sabinene and eucalyptol lures is significantly different from that of the control (f=2.209, df=13, p < 0.05), and the number of insect mouths is 88.33±4.91 heads/plate, 94±16.86 heads/plate and 79.67±6.36 heads/plate in sequence; at a concentration level of 10 μl/mL, the number of compounds with attractant activity on small leafhoppers was increased, and the number of leafhoppers on the armyworms of acetophenone, linalool oxide, α -pinene, eucalyptol, heptadecane and p-cymene lures was significantly higher than that of the control (f=3.184, df=13, p < 0.05); the number of leafhoppers on the 4-compound induced core mythic fungus plates with the concentration of 0.1 mu L/mL concentration level, such as acetophenone, terpinene, linalool oxide and the like is obviously higher than that of a control (F=4.001, df=13, P < 0.05), wherein the number of leafhoppers trapped on the linalool induced core mythic fungus plates is the greatest, and the number of leafhoppers on the mythic fungus plates is up to 107+/-8.72 heads/plate, which is increased compared with the number of leafhoppers on the mythic fungus plates treated by the other three-concentration compounds. The number of leafhoppers on the armyworm plates of the linalool and linalool oxide lures was significantly higher than the control (f=2.440, df=13, p < 0.05) at a concentration level of 0.001 μl/mL. To sum up, after 3d treatment, the number of attractants attracting small leafhoppers on the mythic plates of the lure cores of acetophenone (10, 0.1. Mu.L/mL), terpinene (0.1. Mu.L/mL), linalool (0.1. Mu.L/mL), ocimene (1000. Mu.L/mL), linalool oxide (0.001. Mu.L/mL), sabinene (1000. Mu.L/mL), eucalyptol (1000, 10. Mu.L/mL) and heptadecane (10. Mu.L/mL) was greatly different from the control, with the number of attractants attracting small leafhoppers at most 107.+ -. 8.71 head/plate, and the minimum 80.67.+ -. 6.94 head/plate.
Over time, the compounds that were attractive to leafhoppers at 7d were generally high concentrations. When the concentration is 1000 mul/mL, the compound species acting on the small leafhoppers is more than that under other concentration conditions, namely acetophenone, linalool oxide, alpha-pinene, sabinene and eucalyptol lured the number of leafhoppers on the mythic plates is significantly higher than that of the control (f=4.859, df=13, p < 0.05); at a concentration of 10 μl/mL, the number of leafhoppers on acetophenone and eucalyptol-induced core mythimna separata plates was significantly different from the control (f=1.875, df=13, p < 0.05); at a concentration of 0.1 μl/mL, the number of small through leafhoppers on the armyworm plates of the acetophenone, terpinene, linalool and linalool oxide lures was significantly higher than the control (f=1.956, df=13, p < 0.05); at a concentration of 0.001 μl/mL, the number of small leafhoppers on the 13 camellia volatile components lured armyworm plates was not significant compared to the control (f=0.894, df=13, p > 0.05). In summary, after 7d of treatment, the compounds with higher attracting effect on the lesser leafhoppers are sabinene (1000 mu L/mL), acetophenone (10 mu L/mL), eucalyptol (10 mu L/mL) and terpinene (0.1 mu L/mL), and the number of leafhoppers on the armyworm plates can reach 246.67 +/-44.83 heads/plate at most.
The sources of the small through leafhoppers in the following examples are tea stream cereal agriculture development limited company in mountain east Taishan, the tea tree variety is Fuding Dabai, and the adopted reagents acetophenone, eucalyptol and sabinene are purchased from Shanghai microphone Lin Shenghua limited company.
Example 1
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is 60:1:1.
Example 2
An leafhopper attractant contains acetophenone and eucalyptol as effective components; wherein the volume ratio of acetophenone to eucalyptol is 60:1.
Example 3
An leafhopper attractant contains acetophenone and sabinene as effective components; wherein the volume ratio of acetophenone to sabinene is 60:1.
Example 4
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is 1:1:1.
Example 5
An leafhopper attractant contains acetophenone and eucalyptol as effective components; wherein the volume ratio of acetophenone to eucalyptol is 1:1.
Example 6
An leafhopper attractant contains acetophenone and sabinene as effective components; wherein the volume ratio of acetophenone to sabinene is 1:1.
Example 7
An leafhopper attractant contains eucalyptol and sabinene as effective components; wherein, the volume ratio of the eucalyptol to the sabinene is 1:1.
Example 8
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is 2:1:1.
Example 9
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is 3:1:1.
Example 10
An leafhopper attractant contains acetophenone, eucalyptol and sabinene as effective components; wherein, the volume ratio of acetophenone, eucalyptus oil and sabinene is 10:1:1.
Influence of different attractants on the response of the trending behavior in leafhoppers
10 leafhopper attractant stock solutions of example 1-example 10 (namely, stock solutions of acetophenone, eucalyptol and sabinene are mixed according to a certain volume ratio) are respectively prepared and used for measuring the influence of the attractant stock solution on the indoor trend behavior of the leafhoppers. The method for determining the attractive effect (indoor behavior test) of different camellia volatile matters on leafhoppers is described in the following steps.
The results are shown in FIG. 4, wherein blend1 to blend10 refer to the leafhopper attractants of examples 1 to 10, respectively. As can be seen from FIG. 4, blend8 (χ 2 ＝7.521,df＝1,P<0.01 Has extremely remarkable evasion effect on the lesser leafhoppers,blend1(χ 2 ＝5.272,df＝1,P<0.05)、blend 7(χ 2 ＝5.272,df＝1,P<0.05)、blend 10(χ 2 ＝5.272,df＝1,P<0.05 Has remarkable attracting effect on the lesser leafhoppers.
Based on the above results, the stock solutions of the attractants of the blend1, blend7, blend8 and blend10 with obvious selection effect of the lesser leafhoppers are diluted by liquid paraffin to the concentration of 10 mu L/mL, 0.1 mu L/mL and 0.001 mu L/mL, and the response of the diluted solutions with different concentrations of the attractants to the trend behavior in the lesser leafhoppers is measured, wherein 1000 mu L/mL refers to the stock solution of the attractants. 100. Mu.L of each solution was dropped onto filter paper and used as an odor source for the "Y" tube test, and liquid paraffin was used as a control. The method for determining the attractive effect (indoor behavior test) of different camellia volatile matters on leafhoppers is described in the following steps.
As shown in FIG. 5, the smaller leafhoppers tended to significantly trend toward 1000. Mu.L/mL blend1 (χ 2 ＝5.272,df＝1,P<
0.05 For 0.001. Mu.L/mL of blend1 (χ) 2 ＝5.272,df＝1,P<0.05 Shows a significant evasive response. Pacific Eyew 1000. Mu.L/mL blend7 (χ) 2 ＝5.272,df＝1,P<0.05)、0.1μL/mL blend7(χ 2 ＝5.272,df＝1,P<0.05 Shows a pronounced trend towards selection and is specific to 0.001. Mu.L/mL blend7 (χ) 2 ＝7.521,df＝1,P<0.01 A very pronounced trending reaction. The blue 8 has extremely remarkable evasion effect (χ) on leafhoppers at high concentration 2 ＝7521,df＝1,P<0.01 But as the concentration decreases, the evasion gradually decreases. At 0.1. Mu.L/mL (χ 2 ＝10.221,df＝1,P<0.01 Extremely significantly attracting the lesser leafhoppers, but too low a concentration cannot affect the lesser leafhoppers. The attractant blend10 attracts leafhoppers at four concentrations but is predominantly 1000. Mu.L/mL (χ 2 ＝5.272,df＝1,P<0.05 0.1. Mu.L/mL (χ) 2 ＝5.272,df＝1,P<0.05 Is used for obviously attracting the small-penetration leafhoppers.
Influence of different attractants on leafhopper field trend behavior reaction
10 leafhopper attractant stock solutions of example 1-example 10 were separately prepared and the effect of the different attractant stock solutions on the leafhopper field trend response was determined.
In the field test, 300 mu L of the attractant stock solution is filled into a 2mL slow-release bottle, and then the slow-release bottle is fixed at the center of a double-sided yellow sticky board to perform the field regulation test of the mixed volatile formula on the leafhoppers in the tea garden, and 300 mu L of liquid paraffin is used as a control.
The experiment is carried out in Shandong Taishan mountain tea valley agriculture development limited company, and the tea tree variety is Fuding Dabai'. Yellow adhesive plates (25 cm. Times.20 cm) for field trap experiments were purchased from Engell agriculture technologies Inc. of Zhangzhou. In field test, a double-sided yellow sticky plate is arranged to process single component and mixed component volatile substances, a slow release bottle is attached to the center of the upper edge of the sticky plate through filaments, each sticky plate is repeated 3 times, and a completely random design is adopted. These yellow plates were hung on bamboo poles with the bottom just above the tea tree crown, with a 3m interval between treatments, and a blank control was set. The number of small through leafhoppers on yellow plates was counted after 3d and 7 d.
As a result, as shown in fig. 6, after 3d treatment, the number of leafhoppers trapped on the end1 core-trapping myxoma plate formed by mixing acetophenone, eucalyptol and sabinene in a ratio of 60:1:1 was significantly different from that of the control (f=2.682, df=10, p < 0.05), and the number of leafhoppers can reach 206.33 ±28.83 heads/plate. In the results of the two investigation, the number of the leafhoppers trapped on the myxophore plate of the blend6 trapping core formed by mixing acetophenone and sabinene in a ratio of 1:1 is obviously higher than that of the control myxophore plate (3 d: F=2.682, df=10, P <0.05;7d: F=3.030, df=10, P < 0.05), the number of the leafhoppers trapped after 3d treatment can reach 276+/-24.11 heads/plate, and the number of the leafhoppers trapped after 7d treatment is 437.67 +/-44.73 heads/plate. The number of the leaf hoppers induced by the core 8 treated sticky insect plates formed by mixing acetophenone, eucalyptol and sabinene in a ratio of 2:1:1 is significantly different from that of the control after 3d treatment and 7d treatment (3 d: F=2.682, df=10, P <0.05;7d: F=3.030, df=10, P < 0.05), the number of the leaf hoppers on the sticky insect plates is 213.67 +/-43.97 heads/plate when 3d treatment and the number of the leaf hoppers is 427+/-51.45 heads/plate when 7d treatment. The attraction of the blue 7 consisting of the eucalyptol and sabinene mixed in a ratio of 1:1 to the leafhoppers is enhanced when the treatment is carried out for 7d, and the attraction is remarkably different from that of the control (F=3.030, df=10 and P < 0.05). In the results of the two surveys, none of the other 5 mixed formulas had significant regulatory effects on the leafhoppers.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. A preparation for influencing the trending behavior of leafhoppers, characterized in that the active ingredient comprises at least one of acetophenone, terpinene, linalool, ocimene, linalool oxide, α -pinene, sabinene, eucalyptol, heptadecane and p-cymene.
2. The preparation for influencing the trending behavior of leafhoppers according to claim 1, wherein the active ingredient comprises at least one of acetophenone, terpinene, linalool, sabinene, eucalyptol, heptadecane and p-cymene.
3. The preparation for influencing the trending behavior of leafhoppers according to claim 1 or 2, wherein the concentration of the active ingredient in the preparation is 0.001 to 1000 μl/mL.
4. A formulation affecting the trending behavior of leafhoppers according to claim 3, wherein said active ingredients are at least two of acetophenone, sabinene and eucalyptol.
5. The use of the preparation for influencing leafhopper trending behavior according to claim 4 for the preparation of an leafhopper attractant or leafhopper repellent.
6. An leafhopper attractant is characterized in that the effective components are any one of the following compositions i to iii:
i. acetophenone, eucalyptol and sabinene;
acetophenone and sabinene;
eucalyptol and sabinene.
7. The leafhopper attractant according to claim 6, wherein when the active ingredients are acetophenone, eucalyptol and sabinene composition, the volume ratio of acetophenone, eucalyptol and sabinene is: 2:1:1, 10:1:1 or 60:1:1, the working concentration is 0.1 mu L/mL when the ratio is 2:1:1, the working concentration is 0.1 mu L/mL to 1000 mu L/mL when the ratio is 10:1:1, and the working concentration is 1000 mu L/mL when the ratio is 60:1:1;
when the active ingredients are acetophenone and sabinene compositions, the volume ratio of the acetophenone to sabinene is as follows: 1:1, working concentration is 1000 mu L/mL;
when the active ingredients are the eucalyptus oil and sabinene composition, the volume ratio of the eucalyptus oil to the sabinene is as follows: 1:1, the working concentration is 0.001 mu L/mL-1000 mu L/mL.
8. The leafhopper repellent is characterized in that the active ingredients are acetophenone, eucalyptol and sabinene, and the volume ratio of the acetophenone, the eucalyptol and the sabinene is as follows: 2:1:1 or 60:1:1, when the volume ratio of acetophenone, eucalyptol and sabinene is: at a ratio of 2:1:1, the working concentration is 1000. Mu.L/mL, and when the volume ratio of acetophenone, eucalyptol and sabinene is: at 60:1:1, the working concentration was 0.001. Mu.L/mL.
9. Use of an attractant according to claim 7 or a repellent according to claim 8 for the control of leafhoppers.
10. The use according to claim 6, wherein the leafhoppers are small green leafhoppers.
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