CN115633691A - Use of tannic acid as component of mosquito attractant - Google Patents
Use of tannic acid as component of mosquito attractant Download PDFInfo
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- CN115633691A CN115633691A CN202211243479.2A CN202211243479A CN115633691A CN 115633691 A CN115633691 A CN 115633691A CN 202211243479 A CN202211243479 A CN 202211243479A CN 115633691 A CN115633691 A CN 115633691A
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- China
- Prior art keywords
- attractant
- tannic acid
- component
- aedes albopictus
- acid
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- 239000005667 attractant Substances 0.000 title claims abstract description 47
- 229920002258 tannic acid Polymers 0.000 title claims abstract description 43
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 239000001263 FEMA 3042 Substances 0.000 title claims abstract description 42
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 title claims abstract description 42
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 title claims abstract description 42
- 229940033123 tannic acid Drugs 0.000 title claims abstract description 42
- 235000015523 tannic acid Nutrition 0.000 title claims abstract description 42
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 39
- 241000255925 Diptera Species 0.000 title claims description 30
- 241000256173 Aedes albopictus Species 0.000 claims abstract description 22
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 26
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 24
- 239000004310 lactic acid Substances 0.000 claims description 13
- 235000014655 lactic acid Nutrition 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 229960002446 octanoic acid Drugs 0.000 claims description 12
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229920001864 tannin Polymers 0.000 claims description 2
- 235000018553 tannin Nutrition 0.000 claims description 2
- 239000001648 tannin Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 27
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 abstract description 18
- KWKAKUADMBZCLK-UHFFFAOYSA-N 1-octene Chemical compound CCCCCCC=C KWKAKUADMBZCLK-UHFFFAOYSA-N 0.000 abstract description 11
- 150000001875 compounds Chemical class 0.000 abstract description 6
- 239000002131 composite material Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000011550 stock solution Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 241001502567 Chikungunya virus Species 0.000 description 1
- 241000256059 Culex pipiens Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 229960000448 lactic acid Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- TVMXDCGIABBOFY-UHFFFAOYSA-N n-Octanol Natural products CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of tannic acid as an aedes albopictus attractant component, which has the characteristics of simple and convenient operation, wide effective concentration range, high fault tolerance rate, high attraction efficiency and the like, and simultaneously provides 2 composite attractants, two compounds with better attraction effect on aedes albopictus, namely L-lactic acid and octene, are screened out, the two compounds are printed with the mixture ratio with the best effect for configuration, and the aedes albopictus is attracted by the combined action of the L-lactic acid and the octene to achieve the best effect.
Description
Technical Field
The invention relates to the field of biotechnology, in particular to preparation of a mosquito attractant, and particularly relates to application of tannic acid as a mosquito attractant component.
Background
The aedes albopictus, also called Asian tiger mosquito, is a common mosquito species in south Liaoning China, is a highly aggressive mosquito and an important vector organism, and can transmit various pathogens, including viruses, chikungunya viruses and the like. Aedes albopictus is one of the 100 species which have the fastest diffusion rate globally over the last 20 years, and is one of the main mosquito species for killing mosquitoes in cities.
Female mosquito blood-sucking mosquito species are widely distributed, and the carbon dioxide mosquito trapping lamp is commonly used in mosquito monitoring in China, has a good trapping effect on hemophilus mosquitoes, has good universality, and is a practical device for monitoring hemophilus mosquito population. However, the method does not consider the specificity emitted by different hosts, is difficult to realize the selective trapping of mosquitoes preferred by different hosts, and is not beneficial to accurately reflecting the host preference of the field mosquitoes. The main factor for sucking blood before attracting mosquitoes is odorous substances emitted by hosts, and the difference of the types and the contents of the odorous substances of the hosts is one of the important reasons for the preference of the mosquito hosts.
Disclosure of Invention
According to the invention, the environment-friendly chemical substance combination which has an attraction effect on the Aedes albopictus is screened indoors, the field effect evaluation of the attractant is carried out, the attractant formula combination which has an attraction effect on the Aedes albopictus is screened out, and a comprehensive control approach of the Aedes albopictus taking the environment-friendly attractant as a main body is explored.
In order to solve the technical problems, the invention adopts the technical scheme that:
in a first aspect, the present invention provides the use of tannic acid as a component of a mosquito attractant.
The efficacy component of the mosquito attractant provided by the invention is a single component of tannic acid. A single component of tannic acid can be used as an attractant. Tannic acid generally refers to tannic acid, with the chemical formula C76H52O46, a tannin obtained from gallnut. Is yellow or light brown light amorphous powder or flake; no odor, slight special odor, extremely astringent taste, solubility in water and ethanol, solubility in glycerol, and insolubility in diethyl ether, chloroform or benzene.
In order to further verify the effect, the invention utilizes Aedes albopictus to carry out experiments and provides an environment-friendly mosquito attractant which is characterized in that the efficacy component is a tannic acid component.
Further, the environmentally friendly mosquito attractant comprises a tannic acid component. The attractant is a solution containing the efficacy component, and the solvent of the solution is a volatile solvent. The solvent is ethanol or water.
Further, the concentration of the tannic acid is 0.01-100 mug/muL. The relationship between the concentration of tannic acid and the luring effect is obvious, the good effect is achieved in the interval of 0.01-100 mug/mu L, and the effect is not obvious when the concentration is too low or too high.
Furthermore, in order to select an attractant with a better preparation effect, the invention also selects and tests a plurality of compound attractants, the invention selects the caprylic acid and the lactic acid to carry out the compound attractant test, and a plurality of reports about the mosquito attractants of the caprylic acid and the lactic acid are reported, and the mosquito attractants are known in the field.
The invention provides an environment-friendly aedes albopictus attractant which is characterized by comprising tannic acid and caprylic acid.
The invention provides an environment-friendly aedes albopictus attractant which is characterized by comprising tannic acid and lactic acid.
The invention has the beneficial effects that:
the application of tannic acid as the component of the aedes albopictus attractant has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance rate, high attraction efficiency and the like, 2 composite attractants are provided, two compounds with good attraction effect on the aedes albopictus, namely L-lactic acid and octene, are screened out, the two compounds are printed with the mixture ratio with the best effect to be prepared, and the aedes albopictus is attracted through the combined action of the L-lactic acid and the octene to achieve the best effect.
Detailed Description
The invention provides the use of tannic acid as a component of a mosquito attractant, wherein the efficacy component of the attractant comprises tannic acid. The effective component is a component which plays an attraction effect, and is relative to auxiliary materials, solvents and other attraction components which do not interact with each other and the like without the attraction effect. Each component of the attractant can be conventional commercial products. The use comprises the following steps: use of tannic acid for the preparation of a mosquito attractant product, which may be an attractant or an attractant device; the application of the tannic acid as the mosquito attractant can directly utilize a single volatile matter of the tannic acid to play a role, can also be utilized in a composition form, and can also be utilized through a volatilization device. In short, various direct or indirect uses which only utilize the function of the attractive action of the tannic acid on the mosquitoes belong to the technical scheme to be protected by the invention.
Example 1: single-component attraction effect test of tannic acid, caprylic acid and L-lactic acid
1. Preparation of attractant
The purchased octanoic acid stock solution, L-lactic acid stock solution and tannic acid powder are prepared into solutions with different concentrations by using absolute ethyl alcohol as solvents, the test effects are tested, the concentrations are respectively 0.01 mug/muL, 0.1 mug/muL, 1 mug/muL and 10 mug/muL, and the solutions are placed in a closed freezing storage tube for storage after being fully mixed.
Tannic acid manufacturer: shanghai-sourced leaf Biotechnology, inc.; specification: s30456-250g.
2. Inducing experiment by Y-shaped olfactometer
Four experimental groups with the concentration of 0.01 mug/muL, 0.1 mug/muL, 1 mug/muL and 10 mug/muL and a blank control (absolute ethyl alcohol) group are respectively set up, the experimental groups and the blank control group are placed into odor source emission bottles of 5 olfactometers, 35 aedes albopictus are placed into the olfactometer, and various attractants and appropriate concentrations of the attractants are tested.
3. Selecting the concentration of L-lactic acid with the best attracting effect
The results were: the best attracting effect of the octanoic acid is 21 octanoic acid at the concentration of 1 mug/muL (average value of three times of repetition).
The attracting effect of the L-lactic acid at the concentration of 1 mug/muL is the best, and the attracting effect is 11 (the average value of three times of repetition).
The attracting effect of tannic acid at the concentration of 1 mug/muL is the best, and the attracting effect is 18 tannic acid (the average value of three times of repetition). Wherein the content of the first and second substances,
the attracting effect of tannic acid at 0.01 mug/muL, 0.1 mug/muL and 10 mug/muL is respectively 10, 13 and 16.
Example 2: composite attractant effect test
1. Preparation of attractant
The purchased L-lactic acid stock solution, 1-octene stock solution and tannic acid powder are prepared into a composite solution by using absolute ethyl alcohol as a solvent according to the following components:
caprylic acid + tannic acid combination, concentration of each component: 0.1 mug/muL of octene and 1 mug/muL of tannic acid.
The combination of lactic acid and tannic acid, the concentration of each component is as follows: lactic acid 0.1 mug/muL, tannic acid 1 mug/muL.
The combination of octene + lactic acid, the concentration of each component: 0.1 mug/muL of octene, 1 mug/muL of lactic acid.
The combination of lactic acid, caprylic acid and tannic acid has the following concentration: 0.1 mug/mug L of octene, 0.1 mug/mug L of lactic acid and 1 mug/mug L of tannic acid.
2. Inducing experiment by Y-shaped olfactometer
Four experimental groups and a blank control group (absolute ethyl alcohol) are respectively set up and put into smell source emitting bottles of 5 olfactometers, 35 aedes albopictus are put into the olfactometer, and various attractants and appropriate concentrations thereof are tested.
The results were:
the attracting effect of the caprylic acid and the tannic acid is best as follows: 28 (average repeated three times).
Lactic acid + tannic acid: 10 (average repeated three times).
Octene + lactic acid: 3 (average repeated three times).
Lactic acid + caprylic acid + tannic acid: 7 (average value repeated three times).
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and those skilled in the art should understand that they can make various changes, modifications, additions and substitutions within the spirit and scope of the present invention.
Claims (8)
1. Use of tannic acid as a component of an attractant for mosquitoes.
2. The use as set forth in claim 1 wherein the efficacy component of the mosquito attractant is a tannic acid single component.
3. An environment-friendly mosquito attractant is characterized in that the efficacy component is a tannic acid component.
4. The environmentally friendly aedes albopictus attractant of claim 3, wherein the attractant comprises a tannin component.
5. The environmentally friendly aedes albopictus attractant of claim 3, wherein the attractant is a solution containing the efficacy component, and the solvent of the solution is a volatile solvent.
6. The environmentally friendly aedes albopictus attractant as claimed in claim 5, wherein the solvent is ethanol or water.
7. The environment-friendly Aedes albopictus attractant as claimed in claim 5, wherein the concentration of tannic acid is 0.01-100 μ g/μ L.
8. The environmentally friendly aedes albopictus attractant as claimed in claim 3, further comprising one or 2 components selected from caprylic acid and lactic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243479.2A CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243479.2A CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115633691A true CN115633691A (en) | 2023-01-24 |
| CN115633691B CN115633691B (en) | 2024-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211243479.2A Active CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
Country Status (1)
| Country | Link |
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| CN (1) | CN115633691B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116076511A (en) * | 2023-02-17 | 2023-05-09 | 山东农业大学 | Application of tannic acid in inducing plant to resist insects and preparation for inducing plant to resist insects |
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|---|---|---|---|---|
| CN1345586A (en) * | 2000-09-26 | 2002-04-24 | 肖景东 | Medicine for relieving itching and detumescence |
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN113863004A (en) * | 2021-09-28 | 2021-12-31 | 衣拉拉集团股份有限公司 | Mosquito-repelling antibacterial plant traditional Chinese medicine essential oil composition and preparation method of cotton knitted fabric with mosquito-repelling antibacterial function |
| CN115029925A (en) * | 2022-05-11 | 2022-09-09 | 江苏康溢臣生命科技有限公司 | Plant traditional Chinese medicine composition and preparation method of mosquito-repelling antibacterial mildew-proof cotton fiber thereof |
-
2022
- 2022-10-12 CN CN202211243479.2A patent/CN115633691B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1345586A (en) * | 2000-09-26 | 2002-04-24 | 肖景东 | Medicine for relieving itching and detumescence |
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN113863004A (en) * | 2021-09-28 | 2021-12-31 | 衣拉拉集团股份有限公司 | Mosquito-repelling antibacterial plant traditional Chinese medicine essential oil composition and preparation method of cotton knitted fabric with mosquito-repelling antibacterial function |
| CN115029925A (en) * | 2022-05-11 | 2022-09-09 | 江苏康溢臣生命科技有限公司 | Plant traditional Chinese medicine composition and preparation method of mosquito-repelling antibacterial mildew-proof cotton fiber thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116076511A (en) * | 2023-02-17 | 2023-05-09 | 山东农业大学 | Application of tannic acid in inducing plant to resist insects and preparation for inducing plant to resist insects |
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| Publication number | Publication date |
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| CN115633691B (en) | 2024-03-29 |
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