CN115633696B - Use of tannins as attractant component for stable flies - Google Patents
Use of tannins as attractant component for stable flies Download PDFInfo
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- CN115633696B CN115633696B CN202211243480.5A CN202211243480A CN115633696B CN 115633696 B CN115633696 B CN 115633696B CN 202211243480 A CN202211243480 A CN 202211243480A CN 115633696 B CN115633696 B CN 115633696B
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- 239000005667 attractant Substances 0.000 title claims abstract description 46
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 32
- 241000257226 Muscidae Species 0.000 title description 9
- 229920001864 tannin Polymers 0.000 title description 5
- 239000001648 tannin Substances 0.000 title description 5
- 235000018553 tannin Nutrition 0.000 title description 5
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000001263 FEMA 3042 Substances 0.000 claims abstract description 36
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims abstract description 36
- 229940033123 tannic acid Drugs 0.000 claims abstract description 36
- 235000015523 tannic acid Nutrition 0.000 claims abstract description 36
- 229920002258 tannic acid Polymers 0.000 claims abstract description 36
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims abstract 4
- 241001494115 Stomoxys calcitrans Species 0.000 claims description 3
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 abstract description 38
- 230000000694 effects Effects 0.000 abstract description 24
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 abstract description 19
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 abstract description 19
- 229930007744 linalool Natural products 0.000 abstract description 19
- 239000002131 composite material Substances 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 9
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 40
- LRBQNJMCXXYXIU-QWKBTXIPSA-N gallotannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@H]2[C@@H]([C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-QWKBTXIPSA-N 0.000 description 32
- 229960002446 octanoic acid Drugs 0.000 description 20
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 241000255925 Diptera Species 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- YCCILVSKPBXVIP-UHFFFAOYSA-N 2-(4-hydroxyphenyl)ethanol Chemical compound OCCC1=CC=C(O)C=C1 YCCILVSKPBXVIP-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000004429 Bacillary Dysentery Diseases 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010017915 Gastroenteritis shigella Diseases 0.000 description 1
- DBLDQZASZZMNSL-QMMMGPOBSA-N L-tyrosinol Natural products OC[C@@H](N)CC1=CC=C(O)C=C1 DBLDQZASZZMNSL-QMMMGPOBSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002418 insect attractant Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 210000000196 olfactory nerve Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 201000005113 shigellosis Diseases 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 235000004330 tyrosol Nutrition 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of tannic acid as a stable-rot fly attractant component, and provides a plurality of composite attractants, and simultaneously screens out the composite attractants with good tannic acid and linalool and good stable-rot fly attracting effect, and the two compounds are selected to be prepared in optimal proportioning concentration, and the stable-rot fly is attracted through the combined action of the two substances, so that the optimal effect is achieved.
Description
Technical Field
The invention relates to application of tannic acid as a component of a stable-rot fly attractant, in particular to preparation of a fly attractant, and belongs to the technical field of biology.
Background
Flies are one of important sanitary pests, are various, and are harmful to harassment animals and carry pathogen-transmitted diseases including infectious diseases such as cholera, typhoid fever, bacillary dysentery, anthrax and suppurative coccus infection. Some kinds of larvae can enter human bodies or livestock bodies to cause fly maggots and harm the health of people and livestock. The activity area and the activity mode of the stable rot fly are relatively close to the life of human beings, and the pathogen carried by the stable rot fly is easy to transmit to the human beings, so the stable rot fly is particularly important to control, the fly is mainly controlled by chemical control at present, and although the chemical pesticide has the advantages of quick response, convenient use, suitability for large-scale application and the like, the chemical components in the stable rot fly not only can produce environmental pollution, but also can be unsafe and even toxic to human beings and livestock, and the fly is easy to produce drug resistance to the pesticide. Therefore, finding a simple, safe and environment-friendly fly control technology is a development direction.
The results of the study show that odorants are important environmental information for flies to find spouse, food and spawning sites. The smell substances in the air flow can cause the olfactory nerve cells to generate electrophysiological reaction, and various smell substances have certain attracting effect on flies.
Disclosure of Invention
According to the invention, the environment-friendly chemical substance combination with the attracting effect on the stable flies is screened, the on-site effect evaluation of the attractants is carried out, the attractant formula combination with the attracting effect on the stable flies is screened, and the stable flies comprehensive prevention and control way taking the environment-friendly attractants as the main body is explored.
In order to solve the technical problems, the invention adopts the following technical scheme:
firstly, the invention provides a new application of tannic acid as a component of the fly attractant, and the tannic acid is compounded with other various components to obtain the attractant with better attracting effect.
The efficacy component of the fly attractant provided by the invention is a single component of tannic acid. The single component of tannic acid can be used as an attractant. Tannic acid generally refers to tannic acid, and has chemical formula C 76 H 52 O 46 Is a tannin obtained from Galla chinensis, and is yellow or light brown light amorphous powder or flake; odorless, slightly special smell, extremely astringent, soluble in water and ethanol, easily soluble in glycerol, and almost insoluble in diethyl ether, chloroform or benzene.
To further verify its effectiveness, the present invention was tested with stable flies and provides an environmentally friendly fly attractant characterized in that the efficacy component is a tannic acid component.
Further, the environment-friendly fly attractant comprises a tannic acid component. The attractant is a solution containing the efficacy component, and the solvent of the solution is a volatile solvent. The solvent is ethanol or water.
Further, the concentration of tannic acid is 0.01-100 mug/mug. The relation between the concentration of tannic acid and the attracting effect is obvious, and the tannic acid has good effect in the interval of 0.01-100 mug/mug.
Furthermore, in order to select the attractant with better preparation effect, the invention also selects and tests a plurality of composite attractants, and the invention selects linalool and octanoic acid for the composite attractant test.
The use of linalool for use as an attractant for stable flies is not disclosed. The invention discovers that linalool has very obvious attraction effect on stable rot flies,
octanoic acid is a well known attractant component in the art and several patents have reported the use of octanoic acid on attractants for flies. Chinese CN201110341476.8 reports "tyrosol attractant", which contains caprylic acid component. Because the types of flies are more, each fly has different sensitivity to different components, the invention also tests the attraction experiment of octanoic acid to stable rot flies,
furthermore, the invention provides an environment-friendly stable fly attractant which comprises one or 2 components of linalool and octanoic acid.
The invention has the beneficial effects that:
the application of tannic acid as a stable-rot fly attractant component has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance, high attracting efficiency and the like, 2 composite attractants are provided, two compounds with good effects on stable-rot fly attraction are screened out, the two compounds are selected for configuration, the optimal proportioning concentration is printed, and stable-rot flies are attracted through the combined action of the two substances of linalool and octanoic acid, so that the optimal effect is achieved.
Detailed Description
The present invention provides the use of tannic acid as a component of a fly attractant, wherein the efficacy component of the attractant comprises tannic acid. The efficacy component refers to a component which plays an attractive role, and is relative to auxiliary materials, solvents, other non-interactive attractive components and the like which have no attractive role. The components of the attractant can be conventional commercial products. The use comprises: use of tannic acid for the preparation of a fly attractant product, which may be an attractant or an attracting device; the use of tannins for insect attractants may be effected by direct use of the tannins as a single volatile, or as a combination, or by a volatilizing device. In short, various direct or indirect uses of the function of attracting flies by tannic acid are all the technical proposal to be protected by the invention.
Example 1: single-component attractant effect test of tannic acid, linalool and octanoic acid
1. Preparation of attractants
Linalool, octanoic acid, tannic acid powder of purchase are with absolute ethyl alcohol as the solvent, and preparation concentration is 0.01 mu g/mu l, 0.1 mu g/mu l, 1 mu g/mu l, 10 mu g/mu l respectively, fully mix the back, adorn and preserve in inclosed cryopreservation pipe.
Tannic acid manufacturer: shanghai Yuan Ye Biotech Co., ltd; specification of: s30156-250 g.
2. Attracting experiment by Y-type olfactometer
Setting up four experimental groups and blank control (absolute ethyl alcohol) groups with the concentration of 0.01 mu g/mu l, 0.1 mu g/mu l, 1 mu g/mu l and 10 mu g/mu l respectively, putting the four experimental groups and blank control (absolute ethyl alcohol) groups into odor source emission bottles of 5 smell instruments, putting 25 stable flies into the smell instruments, and testing various attractants and optimal concentrations.
3. Selecting the concentration with the best attracting effect
The results were: the attracting effect of octanoic acid at the concentration of 0.1 mu g/mu l is optimal and is 12 (average value of three times of repetition)
The optimal induction effect of linalool at the concentration of 10 mug/mu l is 17 (average value of three repetitions)
The optimal attraction effect of tannic acid at the concentration of 1 mug/mu l is 15 tannic acid (average value of three times of repetition)
Example 2: composite attractant effect test
1. Preparation of attractants
The purchased linalool, octanoic acid and tannic acid powder are prepared into a composite solution by using absolute ethyl alcohol as a solvent according to the following components:
octanoic acid + tannic acid combination, each component concentration: octanoic acid 0.1 mug/mug, tannic acid 1 mug/mug.
Linalool+tannic acid, concentration of each component: 10 mug/mug of linalool and 1 mug/mug of tannic acid.
Linalool + octanoic acid + tannic acid, each component concentration: octanoic acid 0.1 [ mu ] g/[ mu ] L, linalool 10 [ mu ] g/[ mu ] L, tannic acid 1 [ mu ] g/[ mu ] L.
Octanoic acid + linalool, each component concentration: octanoic acid 0.1 mug/mug, linalool 10 mug/mug.
2. Attracting experiment by Y-type olfactometer
Four experimental groups and a blank control (absolute ethyl alcohol) group are respectively established, the four experimental groups and the blank control (absolute ethyl alcohol) group are placed into odor source emission bottles of 5 olfactory instruments, 25 stable flies are placed into the olfactory instruments, and various attractants and proper concentrations thereof are tested.
The results were:
the best attractant effect of linalool and tannic acid is as follows: 21 (average of three replicates).
Octanoic acid + tannic acid: 10 (average of three replicates).
Linalool + octanoic acid + tannic acid: 13 (average of three replicates).
Octanoic acid + linalool: 7 (average of three replicates).
In terms of effect, the attraction effect of a specific composite attractant is higher than that of a single attractant, and some composite attractants are not as good as the single attractants, probably because the attractants mutually influence each other, and the volatilization of components and the change of smell are influenced.
It should be understood that the above description is not intended to limit the invention to the particular embodiments disclosed, but to limit the invention to the particular embodiments disclosed, and that various changes, modifications, additions and substitutions can be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (2)
1. Use of tannic acid as a component of a stable fly attractant.
2. The use according to claim 1, characterized in that the efficacy component of the stable fly attractant is a single component of tannic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243480.5A CN115633696B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as attractant component for stable flies |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211243480.5A CN115633696B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as attractant component for stable flies |
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| Publication Number | Publication Date |
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| CN115633696A CN115633696A (en) | 2023-01-24 |
| CN115633696B true CN115633696B (en) | 2024-03-29 |
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